Running external command line application(s). This may print messages to stdout and/or stderr. The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist. Command: run_dada_paired.R /tmp/tmpfxkmf8do/forward /tmp/tmpfxkmf8do/reverse /tmp/tmpfxkmf8do/output.tsv.biom /tmp/tmpfxkmf8do/track.tsv /tmp/tmpfxkmf8do/filt_f /tmp/tmpfxkmf8do/filt_r 150 150 13 13 2.0 2.0 2 consensus 1.0 1 1000000 R version 3.5.1 (2018-07-02) Loading required package: Rcpp DADA2: 1.10.0 / Rcpp: 1.0.3 / RcppParallel: 4.4.4 1) Filtering The filter removed all reads: /tmp/tmpfxkmf8do/filt_f/BAQ1370.1.3_57_L001_R1_001.fastq.gz and /tmp/tmpfxkmf8do/filt_r/BAQ1370.1.3_57_L001_R2_001.fastq.gz not written. The filter removed all reads: /tmp/tmpfxkmf8do/filt_f/BAQ1370.3_71_L001_R1_001.fastq.gz and /tmp/tmpfxkmf8do/filt_r/BAQ1370.3_71_L001_R2_001.fastq.gz not written. The filter removed all reads: /tmp/tmpfxkmf8do/filt_f/BAQ1552.2_69_L001_R1_001.fastq.gz and /tmp/tmpfxkmf8do/filt_r/BAQ1552.2_69_L001_R2_001.fastq.gz not written. The filter removed all reads: /tmp/tmpfxkmf8do/filt_f/BAQ895.3_64_L001_R1_001.fastq.gz and /tmp/tmpfxkmf8do/filt_r/BAQ895.3_64_L001_R2_001.fastq.gz not written. The filter removed all reads: /tmp/tmpfxkmf8do/filt_f/YUN3008.1.2_70_L001_R1_001.fastq.gz and /tmp/tmpfxkmf8do/filt_r/YUN3008.1.2_70_L001_R2_001.fastq.gz not written. Some input samples had no reads pass the filter. xx.x..........................x............x........................... 2) Learning Error Rates 5731258 total bases in 41834 reads from 66 samples will be used for learning the error rates. Traceback (most recent call last): File "/home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 257, in denoise_paired run_commands([cmd]) File "/home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 36, in run_commands subprocess.run(cmd, check=True) File "/home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/subprocess.py", line 418, in run output=stdout, stderr=stderr) subprocess.CalledProcessError: Command '['run_dada_paired.R', '/tmp/tmpfxkmf8do/forward', '/tmp/tmpfxkmf8do/reverse', '/tmp/tmpfxkmf8do/output.tsv.biom', '/tmp/tmpfxkmf8do/track.tsv', '/tmp/tmpfxkmf8do/filt_f', '/tmp/tmpfxkmf8do/filt_r', '150', '150', '13', '13', '2.0', '2.0', '2', 'consensus', '1.0', '1', '1000000']' died with . During handling of the above exception, another exception occurred: Traceback (most recent call last): File "/home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/site-packages/q2cli/commands.py", line 328, in __call__ results = action(**arguments) File "", line 2, in denoise_paired File "/home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/site-packages/qiime2/sdk/action.py", line 245, in bound_callable output_types, provenance) File "/home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/site-packages/qiime2/sdk/action.py", line 390, in _callable_executor_ output_views = self._callable(**view_args) File "/home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 272, in denoise_paired " and stderr to learn more." % e.returncode) Exception: An error was encountered while running DADA2 in R (return code -9), please inspect stdout and stderr to learn more.