Running external command line application. This may print messages to stdout and/or stderr. The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist. Command: vsearch --usearch_global /var/folders/y5/2wrthn7s40l3q1f43wfbtz100000gn/T/qiime2-archive-1o9qbfgx/e1b2b1aa-ee63-4dc2-86fc-1873aa55fb2d/data/dna-sequences.fasta --id 0.8 --query_cov 0.8 --strand both --maxaccepts 10 --maxrejects 0 --db /var/folders/y5/2wrthn7s40l3q1f43wfbtz100000gn/T/qiime2-archive-1wymcwaz/2519539a-9f74-4ca6-af8c-31b05cf3be3b/data/dna-sequences.fasta --threads 1 --output_no_hits --blast6out /var/folders/y5/2wrthn7s40l3q1f43wfbtz100000gn/T/tmpdwo6u290 vsearch v2.7.0_macos_x86_64, 8.0GB RAM, 4 cores https://github.com/torognes/vsearch Reading file /var/folders/y5/2wrthn7s40l3q1f43wfbtz100000gn/T/qiime2-archive-1wymcwaz/2519539a-9f74-4ca6-af8c-31b05cf3be3b/data/dna-sequences.fasta 100% 258151422 nt in 178793 seqs, min 500, max 7859, avg 1444 Masking 100% Counting k-mers 100% Creating k-mer index 100% Searching 100% Matching query sequences: 1074 of 1112 (96.58%) Traceback (most recent call last): File "/Users/inga/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/pandas/core/indexes/base.py", line 2898, in get_loc return self._engine.get_loc(casted_key) File "pandas/_libs/index.pyx", line 70, in pandas._libs.index.IndexEngine.get_loc File "pandas/_libs/index.pyx", line 101, in pandas._libs.index.IndexEngine.get_loc File "pandas/_libs/hashtable_class_helper.pxi", line 1675, in pandas._libs.hashtable.PyObjectHashTable.get_item File "pandas/_libs/hashtable_class_helper.pxi", line 1683, in pandas._libs.hashtable.PyObjectHashTable.get_item KeyError: 'GU823154.1.1395_U' The above exception was the direct cause of the following exception: Traceback (most recent call last): File "/Users/inga/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/q2_feature_classifier/_consensus_assignment.py", line 105, in _import_blast_format_assignments t = ref_taxa[id_] File "/Users/inga/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/pandas/core/series.py", line 882, in __getitem__ return self._get_value(key) File "/Users/inga/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/pandas/core/series.py", line 990, in _get_value loc = self.index.get_loc(label) File "/Users/inga/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/pandas/core/indexes/base.py", line 2900, in get_loc raise KeyError(key) from err KeyError: 'GU823154.1.1395_U' During handling of the above exception, another exception occurred: Traceback (most recent call last): File "/Users/inga/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/q2cli/commands.py", line 329, in __call__ results = action(**arguments) File "", line 2, in classify_consensus_vsearch File "/Users/inga/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/qiime2/sdk/action.py", line 245, in bound_callable output_types, provenance) File "/Users/inga/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/qiime2/sdk/action.py", line 390, in _callable_executor_ output_views = self._callable(**view_args) File "/Users/inga/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/q2_feature_classifier/_vsearch.py", line 64, in classify_consensus_vsearch unassignable_label=unassignable_label) File "/Users/inga/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/q2_feature_classifier/_consensus_assignment.py", line 30, in _consensus_assignments output.name, ref_taxa, unassignable_label=unassignable_label) File "/Users/inga/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/q2_feature_classifier/_consensus_assignment.py", line 110, in _import_blast_format_assignments 'taxonomy.').format(str(id_))) KeyError: 'Identifier GU823154.1.1395_U was reported in taxonomic search results, but was not present in the reference taxonomy.'