qiime2-q2cli-err-unjycmoj.log Running external command line application(s). This may print messages to stdout and/or stderr. The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist. Command: run_dada_single.R /tmp/qiime2-archive-ulqqlkf4/565da43f-946f-49dd-9350-117cdadf0943/data /tmp/tmpag6kzr0y/output.tsv.biom /tmp/tmpag6kzr0y/track.tsv /tmp/tmpag6kzr0y 150 0 2.0 2 Inf consensus 1.0 1 1000000 NULL 16 Error: 1:1: unexpected input 1: ? ^ Execution halted Traceback (most recent call last): File "/seqdata/anaconda2/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 177, in _denoise_single run_commands([cmd]) File "/seqdata/anaconda2/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 36, in run_commands subprocess.run(cmd, check=True) File "/seqdata/anaconda2/envs/qiime2-2020.2/lib/python3.6/subprocess.py", line 418, in run output=stdout, stderr=stderr) subprocess.CalledProcessError: Command '['run_dada_single.R', '/tmp/qiime2-archive-ulqqlkf4/565da43f-946f-49dd-9350-117cdadf0943/data', '/tmp/tmpag6kzr0y/output.tsv.biom', '/tmp/tmpag6kzr0y/track.tsv', '/tmp/tmpag6kzr0y', '150', '0', '2.0', '2', 'Inf', 'consensus', '1.0', '1', '1000000', 'NULL', '16']' returned non-zero exit status 1. During handling of the above exception, another exception occurred: Traceback (most recent call last): File "/seqdata/anaconda2/envs/qiime2-2020.2/lib/python3.6/site-packages/q2cli/commands.py", line 328, in __call__ results = action(**arguments) File "", line 2, in denoise_single File "/seqdata/anaconda2/envs/qiime2-2020.2/lib/python3.6/site-packages/qiime2/sdk/action.py", line 245, in bound_callable output_types, provenance) File "/seqdata/anaconda2/envs/qiime2-2020.2/lib/python3.6/site-packages/qiime2/sdk/action.py", line 390, in _callable_executor_ output_views = self._callable(**view_args) File "/seqdata/anaconda2/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 212, in denoise_single band_size='16') File "/seqdata/anaconda2/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 188, in _denoise_single " and stderr to learn more." % e.returncode) Exception: An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.