This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C1_0_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C1_192_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C1_0_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C1_192_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.32 s (246 us/read; 0.24 M reads/minute). === Summary === Total read pairs processed: 1,302 Read 1 with adapter: 815 (62.6%) Read 2 with adapter: 806 (61.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,302 (100.0%) Total basepairs processed: 582,138 bp Read 1: 290,789 bp Read 2: 291,349 bp Total written (filtered): 541,950 bp (93.1%) Read 1: 270,567 bp Read 2: 271,383 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 314 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.9% C: 2.2% G: 1.9% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 5 20.3 0 5 24 2 0.0 2 2 25 307 0.0 2 306 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 501 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 20.3 0 1 23 2 0.0 2 1 0 1 24 17 0.0 2 2 15 25 479 0.0 2 453 24 2 26 2 0.0 2 1 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 295 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 1.7% C: 0.0% G: 84.7% T: 13.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 294 0.0 2 291 1 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 511 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 6 20.3 0 6 4 2 5.1 0 2 24 18 0.0 2 3 14 1 25 484 0.0 2 471 13 34 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C10_1_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C10_193_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C10_1_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C10_193_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 6.07 s (140 us/read; 0.43 M reads/minute). === Summary === Total read pairs processed: 43,405 Read 1 with adapter: 42,352 (97.6%) Read 2 with adapter: 42,341 (97.5%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 43,405 (100.0%) Total basepairs processed: 21,537,190 bp Read 1: 10,768,917 bp Read 2: 10,768,273 bp Total written (filtered): 19,424,493 bp (90.2%) Read 1: 9,712,716 bp Read 2: 9,711,777 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 499 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 87.2% C: 8.6% G: 4.2% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 16 678.2 0 16 23 1 0.0 2 1 24 6 0.0 2 4 2 25 474 0.0 2 469 5 26 1 0.0 2 1 113 1 0.0 2 0 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 41853 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 678.2 0 3 4 1 169.6 0 1 5 1 42.4 0 1 6 1 10.6 0 1 14 2 0.0 1 0 2 17 1 0.0 1 0 1 18 1 0.0 1 1 19 4 0.0 1 4 20 2 0.0 2 0 0 2 21 8 0.0 2 4 4 22 16 0.0 2 9 2 5 23 139 0.0 2 16 38 85 24 1827 0.0 2 288 1495 44 25 39799 0.0 2 38070 1682 47 26 44 0.0 2 28 15 1 27 3 0.0 2 0 0 3 29 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 468 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 3.0% C: 4.7% G: 68.8% T: 23.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 16 1 0.0 1 1 17 3 0.0 1 2 1 23 1 0.0 2 0 0 1 24 6 0.0 2 4 2 25 455 0.0 2 436 16 3 63 1 0.0 2 1 64 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 41873 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 25 678.2 0 25 4 1 169.6 0 1 5 2 42.4 0 2 15 1 0.0 1 0 1 19 2 0.0 1 2 20 4 0.0 2 3 1 21 8 0.0 2 6 0 2 22 14 0.0 2 7 1 6 23 84 0.0 2 12 11 61 24 1242 0.0 2 213 997 32 25 40443 0.0 2 39148 1270 25 26 41 0.0 2 27 13 1 27 2 0.0 2 1 0 1 28 1 0.0 2 0 0 1 29 1 0.0 2 1 31 1 0.0 2 1 39 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C103_2_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C103_194_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C103_2_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C103_194_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 7.52 s (144 us/read; 0.42 M reads/minute). === Summary === Total read pairs processed: 52,254 Read 1 with adapter: 51,714 (99.0%) Read 2 with adapter: 51,733 (99.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 52,254 (100.0%) Total basepairs processed: 25,966,391 bp Read 1: 13,001,656 bp Read 2: 12,964,735 bp Total written (filtered): 23,385,077 bp (90.1%) Read 1: 11,711,680 bp Read 2: 11,673,397 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 286 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.2% C: 14.7% G: 8.4% T: 0.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 8 816.5 0 8 20 1 0.0 2 1 23 2 0.0 2 2 24 4 0.0 2 0 3 1 25 268 0.0 2 262 6 26 2 0.0 2 2 87 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 51428 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 816.5 0 4 4 1 204.1 0 1 16 1 0.0 1 0 1 19 1 0.0 1 1 20 3 0.0 2 1 1 1 21 10 0.0 2 5 1 4 22 22 0.0 2 7 8 7 23 169 0.0 2 15 29 125 24 2254 0.0 2 362 1781 111 25 48889 0.0 2 46183 2643 63 26 70 0.0 2 35 34 1 27 3 0.0 2 0 0 3 37 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 239 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.7% C: 3.3% G: 54.4% T: 35.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 23 2 0.0 2 1 0 1 24 3 0.0 2 2 1 25 234 0.0 2 216 14 4 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 51494 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 9 816.5 0 9 5 2 51.0 0 2 15 1 0.0 1 1 16 1 0.0 1 0 1 18 1 0.0 1 0 1 19 2 0.0 1 1 1 20 2 0.0 2 1 1 21 3 0.0 2 2 1 22 17 0.0 2 6 4 7 23 107 0.0 2 20 8 79 24 1521 0.0 2 260 1222 39 25 49771 0.0 2 48159 1574 38 26 50 0.0 2 23 26 1 27 1 0.0 2 0 0 1 28 1 0.0 2 1 29 2 0.0 2 2 38 1 0.0 2 0 1 39 2 0.0 2 2 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C104_3_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C104_195_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C104_3_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C104_195_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 4.67 s (152 us/read; 0.40 M reads/minute). === Summary === Total read pairs processed: 30,756 Read 1 with adapter: 28,405 (92.4%) Read 2 with adapter: 28,103 (91.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 30,756 (100.0%) Total basepairs processed: 14,471,460 bp Read 1: 7,213,675 bp Read 2: 7,257,785 bp Total written (filtered): 13,063,171 bp (90.3%) Read 1: 6,505,449 bp Read 2: 6,557,722 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 3427 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 91.9% C: 3.9% G: 3.9% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 25 480.6 0 25 4 2 120.1 0 2 19 1 0.0 1 0 1 23 1 0.0 2 1 24 23 0.0 2 15 8 25 3369 0.0 2 3344 25 26 2 0.0 2 1 1 112 2 0.0 2 2 113 2 0.0 2 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 24978 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 18 480.6 0 18 4 3 120.1 0 3 7 1 1.9 0 1 16 2 0.0 1 0 2 19 3 0.0 1 1 2 21 5 0.0 2 4 0 1 22 11 0.0 2 3 2 6 23 88 0.0 2 10 12 66 24 1042 0.0 2 150 829 63 25 23658 0.0 2 22481 1140 37 26 138 0.0 2 7 120 11 27 9 0.0 2 1 0 8 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 3286 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 3.3% C: 1.5% G: 82.2% T: 13.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 480.6 0 1 4 3 120.1 0 3 11 1 0.0 1 0 1 12 1 0.0 1 1 19 5 0.0 1 3 2 21 10 0.0 2 4 5 1 22 18 0.0 2 7 6 5 23 12 0.0 2 6 0 6 24 48 0.0 2 29 15 4 25 3185 0.0 2 3042 128 15 26 2 0.0 2 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 24817 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 62 480.6 0 62 4 4 120.1 0 4 18 1 0.0 1 0 1 19 1 0.0 1 0 1 20 3 0.0 2 1 1 1 21 2 0.0 2 1 0 1 22 6 0.0 2 1 1 4 23 55 0.0 2 9 9 37 24 683 0.0 2 99 567 17 25 23922 0.0 2 23137 767 18 26 75 0.0 2 14 61 27 1 0.0 2 1 28 1 0.0 2 1 34 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C105_4_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C105_196_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C105_4_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C105_196_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 16.46 s (146 us/read; 0.41 M reads/minute). === Summary === Total read pairs processed: 112,482 Read 1 with adapter: 110,645 (98.4%) Read 2 with adapter: 110,443 (98.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 112,482 (100.0%) Total basepairs processed: 55,648,353 bp Read 1: 27,809,622 bp Read 2: 27,838,731 bp Total written (filtered): 50,133,306 bp (90.1%) Read 1: 25,051,039 bp Read 2: 25,082,267 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 2208 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.0% C: 1.6% G: 2.2% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 64 1757.5 0 64 4 2 439.4 0 2 18 1 0.0 1 1 21 1 0.0 2 1 23 2 0.0 2 1 1 24 18 0.0 2 10 8 25 2117 0.0 2 2107 10 26 2 0.0 2 2 112 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 108437 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 18 1757.5 0 18 4 1 439.4 0 1 5 2 109.8 0 2 7 2 6.9 0 2 15 3 0.0 1 1 2 16 1 0.0 1 1 17 3 0.0 1 2 1 18 3 0.0 1 1 2 19 9 0.0 1 8 1 20 11 0.0 2 7 2 2 21 22 0.0 2 14 3 5 22 45 0.0 2 16 9 20 23 425 0.0 2 48 86 291 24 4595 0.0 2 681 3594 320 25 103094 0.0 2 92790 10023 281 26 202 0.0 2 78 122 2 29 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 1981 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 3.1% C: 0.7% G: 91.2% T: 5.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 1757.5 0 1 4 1 439.4 0 1 11 1 0.0 1 1 13 1 0.0 1 1 16 1 0.0 1 0 1 17 1 0.0 1 1 19 2 0.0 1 2 22 3 0.0 2 3 23 3 0.0 2 2 1 24 29 0.0 2 18 3 8 25 1928 0.0 2 1849 67 12 26 4 0.0 2 4 29 1 0.0 2 1 39 2 0.0 2 1 1 53 1 0.0 2 0 1 74 1 0.0 2 1 113 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 108462 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 44 1757.5 0 44 4 8 439.4 0 8 5 1 109.8 0 1 7 1 6.9 0 1 16 1 0.0 1 1 20 2 0.0 2 0 1 1 21 8 0.0 2 4 2 2 22 29 0.0 2 16 4 9 23 225 0.0 2 29 38 158 24 3162 0.0 2 552 2538 72 25 104747 0.0 2 101575 3110 62 26 230 0.0 2 69 157 4 28 2 0.0 2 2 29 1 0.0 2 0 1 39 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C110_5_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C110_197_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C110_5_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C110_197_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.35 s (171 us/read; 0.35 M reads/minute). === Summary === Total read pairs processed: 2,036 Read 1 with adapter: 1,828 (89.8%) Read 2 with adapter: 1,782 (87.5%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 2,036 (100.0%) Total basepairs processed: 917,836 bp Read 1: 458,563 bp Read 2: 459,273 bp Total written (filtered): 827,864 bp (90.2%) Read 1: 413,015 bp Read 2: 414,849 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 521 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 98.1% C: 1.2% G: 0.4% T: 0.4% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 3 31.8 0 3 24 7 0.0 2 5 2 25 511 0.0 2 506 5 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 1307 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 19 1 0.0 1 1 23 5 0.0 2 1 0 4 24 68 0.0 2 11 55 2 25 1229 0.0 2 1157 68 4 26 3 0.0 2 2 1 27 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 512 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.1% C: 1.2% G: 92.8% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 0 0 1 23 1 0.0 2 1 24 5 0.0 2 3 2 25 501 0.0 2 487 12 2 26 1 0.0 2 1 31 1 0.0 2 1 43 1 0.0 2 1 74 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 1270 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 7 31.8 0 7 23 3 0.0 2 0 0 3 24 31 0.0 2 3 27 1 25 1227 0.0 2 1186 40 1 26 2 0.0 2 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C12_6_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C12_198_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C12_6_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C12_198_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.88 s (178 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 4,977 Read 1 with adapter: 4,696 (94.4%) Read 2 with adapter: 4,693 (94.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 4,977 (100.0%) Total basepairs processed: 2,452,099 bp Read 1: 1,225,797 bp Read 2: 1,226,302 bp Total written (filtered): 2,217,776 bp (90.4%) Read 1: 1,108,566 bp Read 2: 1,109,210 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 76 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.3% C: 21.1% G: 2.6% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 77.8 0 1 23 1 0.0 2 0 0 1 24 1 0.0 2 0 1 25 72 0.0 2 71 1 112 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 4620 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 77.8 0 1 20 2 0.0 2 1 1 22 3 0.0 2 1 0 2 23 20 0.0 2 2 5 13 24 185 0.0 2 20 153 12 25 4375 0.0 2 3788 576 11 26 33 0.0 2 4 27 2 27 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 62 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 12.9% C: 1.6% G: 46.8% T: 38.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 0 1 25 60 0.0 2 54 4 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 4631 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 77.8 0 2 4 1 19.4 0 1 22 1 0.0 2 1 23 16 0.0 2 0 4 12 24 139 0.0 2 25 111 3 25 4464 0.0 2 4325 138 1 26 8 0.0 2 3 5 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C13_7_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C13_199_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C13_7_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C13_199_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 12.20 s (156 us/read; 0.38 M reads/minute). === Summary === Total read pairs processed: 78,122 Read 1 with adapter: 77,061 (98.6%) Read 2 with adapter: 77,094 (98.7%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 78,122 (100.0%) Total basepairs processed: 38,829,039 bp Read 1: 19,420,528 bp Read 2: 19,408,511 bp Total written (filtered): 34,982,753 bp (90.1%) Read 1: 17,498,638 bp Read 2: 17,484,115 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 429 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.1% C: 8.2% G: 12.8% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 19 1220.7 0 19 4 2 305.2 0 2 5 1 76.3 0 1 23 1 0.0 2 0 0 1 24 5 0.0 2 3 2 25 401 0.0 2 397 4 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 76632 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 8 1220.7 0 8 9 1 0.3 0 1 14 1 0.0 1 1 15 1 0.0 1 1 18 2 0.0 1 1 1 19 10 0.0 1 8 0 2 20 11 0.0 2 6 3 2 21 13 0.0 2 8 2 3 22 32 0.0 2 13 5 14 23 247 0.0 2 18 43 186 24 3263 0.0 2 452 2601 210 25 72947 0.0 2 65540 7149 258 26 95 0.0 2 43 47 5 29 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 463 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 13.0% C: 0.4% G: 75.4% T: 11.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 9 0.0 2 3 3 3 25 453 0.0 2 408 39 6 219 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 76631 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 22 1220.7 0 22 4 1 305.2 0 1 5 1 76.3 0 1 18 1 0.0 1 1 19 3 0.0 1 2 0 1 20 6 0.0 2 5 0 1 21 10 0.0 2 4 5 1 22 22 0.0 2 14 3 5 23 165 0.0 2 17 30 118 24 2227 0.0 2 339 1827 61 25 74075 0.0 2 71792 2243 40 26 94 0.0 2 52 37 5 27 3 0.0 2 1 0 2 29 1 0.0 2 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C14_8_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C14_200_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C14_8_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C14_200_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.20 s (185 us/read; 0.32 M reads/minute). === Summary === Total read pairs processed: 1,085 Read 1 with adapter: 632 (58.2%) Read 2 with adapter: 630 (58.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,085 (100.0%) Total basepairs processed: 451,647 bp Read 1: 225,564 bp Read 2: 226,083 bp Total written (filtered): 420,541 bp (93.1%) Read 1: 209,865 bp Read 2: 210,676 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 226 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.1% C: 19.0% G: 4.9% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 17.0 0 3 24 3 0.0 2 2 1 25 220 0.0 2 218 1 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 406 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 36 0.0 2 4 14 18 25 367 0.0 2 341 22 4 26 2 0.0 2 1 1 27 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 203 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.8% C: 1.0% G: 57.6% T: 29.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 17.0 0 1 21 1 0.0 2 0 0 1 22 3 0.0 2 2 0 1 23 2 0.0 2 2 24 3 0.0 2 0 1 2 25 193 0.0 2 173 18 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 427 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 12 17.0 0 12 5 1 1.1 0 1 23 1 0.0 2 1 24 16 0.0 2 3 12 1 25 396 0.0 2 380 15 1 26 1 0.0 2 0 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C19_9_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C19_201_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C19_9_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C19_201_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 5.25 s (166 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 31,578 Read 1 with adapter: 24,623 (78.0%) Read 2 with adapter: 24,241 (76.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 31,578 (100.0%) Total basepairs processed: 14,704,375 bp Read 1: 7,358,419 bp Read 2: 7,345,956 bp Total written (filtered): 13,491,459 bp (91.8%) Read 1: 6,747,600 bp Read 2: 6,743,859 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 4949 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 92.1% C: 4.3% G: 3.4% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 133 493.4 0 133 4 4 123.4 0 4 17 1 0.0 1 1 22 2 0.0 2 2 23 2 0.0 2 2 24 37 0.0 2 30 7 25 4764 0.0 2 4747 17 34 3 0.0 2 3 112 1 0.0 2 1 113 2 0.0 2 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 19674 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 40 493.4 0 40 4 8 123.4 0 8 5 1 30.8 0 1 18 2 0.0 1 2 19 1 0.0 1 1 20 3 0.0 2 2 1 21 2 0.0 2 1 0 1 22 5 0.0 2 0 1 4 23 61 0.0 2 4 12 45 24 779 0.0 2 132 616 31 25 18727 0.0 2 17862 820 45 26 44 0.0 2 12 30 2 27 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 4742 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.2% C: 3.1% G: 87.1% T: 5.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 3 493.4 0 3 4 2 123.4 0 2 13 2 0.0 1 2 16 6 0.0 1 4 2 17 3 0.0 1 3 19 1 0.0 1 0 1 20 3 0.0 2 1 2 22 1 0.0 2 1 23 7 0.0 2 5 2 24 73 0.0 2 44 12 17 25 4611 0.0 2 4382 200 29 26 5 0.0 2 5 32 3 0.0 2 3 34 17 0.0 2 17 46 2 0.0 2 0 2 54 1 0.0 2 0 0 1 61 1 0.0 2 1 113 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 19499 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 125 493.4 0 125 4 23 123.4 0 23 5 6 30.8 0 6 19 1 0.0 1 0 0 1 21 1 0.0 2 0 0 1 22 4 0.0 2 2 1 1 23 42 0.0 2 5 8 29 24 552 0.0 2 104 428 20 25 18722 0.0 2 18173 540 9 26 22 0.0 2 11 11 33 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C20_10_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C20_202_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C20_10_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C20_202_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.12 s (229 us/read; 0.26 M reads/minute). === Summary === Total read pairs processed: 517 Read 1 with adapter: 47 (9.1%) Read 2 with adapter: 48 (9.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 517 (100.0%) Total basepairs processed: 246,498 bp Read 1: 123,230 bp Read 2: 123,268 bp Total written (filtered): 244,539 bp (99.2%) Read 1: 122,253 bp Read 2: 122,286 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 32 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 65.6% C: 12.5% G: 21.9% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 8 8.1 0 8 4 1 2.0 0 1 25 23 0.0 2 20 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 15 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 14 0.0 2 11 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 24 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.7% C: 0.0% G: 4.2% T: 79.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 5 1 0.5 0 1 25 23 0.0 2 19 3 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 24 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 9 8.1 0 9 25 15 0.0 2 14 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C21_11_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C21_203_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C21_11_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C21_203_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.46 s (176 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 2,600 Read 1 with adapter: 1,400 (53.8%) Read 2 with adapter: 1,357 (52.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 2,600 (100.0%) Total basepairs processed: 1,165,279 bp Read 1: 582,362 bp Read 2: 582,917 bp Total written (filtered): 1,096,713 bp (94.1%) Read 1: 547,528 bp Read 2: 549,185 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 478 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 89.3% C: 8.2% G: 2.5% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 4 40.6 0 4 25 473 0.0 2 469 4 26 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 922 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 40.6 0 2 23 2 0.0 2 0 0 2 24 32 0.0 2 9 21 2 25 885 0.0 2 827 56 2 26 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 461 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 8.7% C: 0.9% G: 80.3% T: 10.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 17 1 0.0 1 1 24 9 0.0 2 6 1 2 25 450 0.0 2 430 18 2 26 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 896 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 6 40.6 0 6 4 1 10.2 0 1 24 27 0.0 2 3 24 25 859 0.0 2 843 15 1 26 3 0.0 2 0 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C22_12_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C22_204_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C22_12_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C22_204_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 35.68 s (154 us/read; 0.39 M reads/minute). === Summary === Total read pairs processed: 231,632 Read 1 with adapter: 225,583 (97.4%) Read 2 with adapter: 225,692 (97.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 231,632 (100.0%) Total basepairs processed: 114,679,210 bp Read 1: 57,070,489 bp Read 2: 57,608,721 bp Total written (filtered): 103,423,004 bp (90.2%) Read 1: 51,445,531 bp Read 2: 51,977,473 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 1743 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 83.1% C: 7.2% G: 9.1% T: 0.6% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 128 3619.2 0 128 4 2 904.8 0 2 24 17 0.0 2 12 5 25 1591 0.0 2 1569 20 2 26 2 0.0 2 2 113 2 0.0 2 2 126 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 223840 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 32 3619.2 0 32 4 6 904.8 0 6 8 1 3.5 0 1 12 1 0.0 1 1 13 1 0.0 1 1 14 2 0.0 1 2 15 5 0.0 1 2 3 16 2 0.0 1 0 2 17 5 0.0 1 2 3 18 3 0.0 1 1 2 19 26 0.0 1 21 4 1 20 23 0.0 2 13 4 6 21 43 0.0 2 23 8 12 22 83 0.0 2 32 11 40 23 796 0.0 2 106 147 543 24 9057 0.0 2 1341 7326 390 25 213437 0.0 2 201140 12011 286 26 307 0.0 2 192 110 5 27 3 0.0 2 1 0 2 28 2 0.0 2 1 1 29 4 0.0 2 4 33 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 1632 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.5% C: 0.9% G: 73.3% T: 20.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 3619.2 0 5 4 1 904.8 0 1 16 3 0.0 1 3 21 2 0.0 2 1 1 22 2 0.0 2 0 2 23 1 0.0 2 0 1 24 22 0.0 2 12 6 4 25 1595 0.0 2 1502 80 13 113 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 224060 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 115 3619.2 0 115 4 23 904.8 0 23 5 5 226.2 0 5 6 1 56.6 0 1 7 1 14.1 0 1 13 1 0.0 1 1 15 1 0.0 1 1 18 4 0.0 1 2 2 19 1 0.0 1 1 20 13 0.0 2 7 5 1 21 27 0.0 2 13 8 6 22 75 0.0 2 26 17 32 23 481 0.0 2 65 81 335 24 6731 0.0 2 1124 5445 162 25 216289 0.0 2 209791 6344 154 26 274 0.0 2 167 101 6 27 5 0.0 2 1 0 4 28 5 0.0 2 3 2 29 5 0.0 2 4 1 31 1 0.0 2 1 39 2 0.0 2 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C23_13_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C23_205_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C23_13_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C23_205_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.05 s (220 us/read; 0.27 M reads/minute). === Summary === Total read pairs processed: 213 Read 1 with adapter: 133 (62.4%) Read 2 with adapter: 126 (59.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 213 (100.0%) Total basepairs processed: 90,894 bp Read 1: 45,485 bp Read 2: 45,409 bp Total written (filtered): 84,446 bp (92.9%) Read 1: 42,163 bp Read 2: 42,283 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 45 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 82.2% C: 11.1% G: 4.4% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 3 3.3 0 3 25 41 0.0 2 41 113 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 88 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 3.3 0 1 24 3 0.0 2 0 3 25 84 0.0 2 75 9 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 46 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 13.0% C: 0.0% G: 76.1% T: 10.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 1 25 45 0.0 2 38 6 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 80 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 3.3 0 1 25 79 0.0 2 78 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C30_14_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C30_206_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C30_14_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C30_206_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.06 s (219 us/read; 0.27 M reads/minute). === Summary === Total read pairs processed: 272 Read 1 with adapter: 87 (32.0%) Read 2 with adapter: 87 (32.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 272 (100.0%) Total basepairs processed: 131,007 bp Read 1: 65,491 bp Read 2: 65,516 bp Total written (filtered): 126,888 bp (96.9%) Read 1: 63,431 bp Read 2: 63,457 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 5 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 40.0% G: 60.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 4.2 0 3 25 2 0.0 2 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 82 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 4.2 0 2 22 1 0.0 2 1 24 2 0.0 2 1 1 25 77 0.0 2 66 9 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 8 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 8 0.0 2 0 6 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 79 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 4.2 0 4 4 1 1.1 0 1 24 7 0.0 2 2 5 25 67 0.0 2 65 2 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C31_15_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C31_207_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C31_15_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C31_207_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.15 s (170 us/read; 0.35 M reads/minute). === Summary === Total read pairs processed: 868 Read 1 with adapter: 776 (89.4%) Read 2 with adapter: 773 (89.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 868 (100.0%) Total basepairs processed: 411,757 bp Read 1: 205,950 bp Read 2: 205,807 bp Total written (filtered): 372,976 bp (90.6%) Read 1: 186,419 bp Read 2: 186,557 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 70 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 98.6% C: 0.0% G: 1.4% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 67 0.0 2 67 112 1 0.0 2 1 113 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 706 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 4 0.0 2 1 1 2 24 35 0.0 2 10 23 2 25 667 0.0 2 636 30 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 74 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 10.8% C: 1.4% G: 87.8% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 73 0.0 2 64 8 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 699 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 13.6 0 2 22 1 0.0 2 0 1 23 2 0.0 2 0 0 2 24 23 0.0 2 4 17 2 25 671 0.0 2 647 22 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C33_16_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C33_208_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C33_16_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C33_208_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.06 s (185 us/read; 0.32 M reads/minute). === Summary === Total read pairs processed: 350 Read 1 with adapter: 261 (74.6%) Read 2 with adapter: 259 (74.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 350 (100.0%) Total basepairs processed: 148,235 bp Read 1: 74,067 bp Read 2: 74,168 bp Total written (filtered): 135,225 bp (91.2%) Read 1: 67,582 bp Read 2: 67,643 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 81 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 79.0% C: 18.5% G: 2.5% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 5.5 0 1 21 1 0.0 2 1 24 2 0.0 2 1 1 25 77 0.0 2 76 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 180 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 12 0.0 2 3 8 1 25 168 0.0 2 161 7 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 63 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 1.6% C: 0.0% G: 77.8% T: 20.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 16 1 0.0 1 1 24 1 0.0 2 0 1 25 60 0.0 2 58 2 113 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 196 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 5.5 0 1 21 1 0.0 2 1 23 1 0.0 2 0 1 24 2 0.0 2 1 1 25 190 0.0 2 186 4 27 1 0.0 2 0 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C34_17_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C34_209_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C34_17_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C34_209_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.15 s (170 us/read; 0.35 M reads/minute). === Summary === Total read pairs processed: 909 Read 1 with adapter: 697 (76.7%) Read 2 with adapter: 690 (75.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 909 (100.0%) Total basepairs processed: 419,190 bp Read 1: 209,562 bp Read 2: 209,628 bp Total written (filtered): 384,609 bp (91.8%) Read 1: 192,160 bp Read 2: 192,449 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 153 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 94.1% C: 5.2% G: 0.7% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 22 1 0.0 2 1 24 1 0.0 2 1 25 150 0.0 2 148 2 28 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 544 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 22 0.0 2 3 18 1 25 522 0.0 2 491 25 6 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 136 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.8% C: 0.7% G: 75.0% T: 12.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 0 1 25 134 0.0 2 129 3 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 554 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 14.2 0 2 22 1 0.0 2 1 23 3 0.0 2 1 2 24 17 0.0 2 4 13 25 530 0.0 2 521 9 26 1 0.0 2 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C35_18_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C35_210_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C35_18_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C35_210_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 2.13 s (142 us/read; 0.42 M reads/minute). === Summary === Total read pairs processed: 14,989 Read 1 with adapter: 12,015 (80.2%) Read 2 with adapter: 11,973 (79.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 14,989 (100.0%) Total basepairs processed: 7,209,444 bp Read 1: 3,600,675 bp Read 2: 3,608,769 bp Total written (filtered): 6,613,147 bp (91.7%) Read 1: 3,302,047 bp Read 2: 3,311,100 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 1117 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 89.3% C: 5.1% G: 4.3% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 47 234.2 0 47 4 2 58.6 0 2 24 7 0.0 2 6 1 25 1057 0.0 2 1053 4 26 2 0.0 2 2 111 1 0.0 2 1 112 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 10898 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 13 234.2 0 13 4 2 58.6 0 2 20 1 0.0 2 0 0 1 21 3 0.0 2 2 0 1 22 7 0.0 2 2 2 3 23 34 0.0 2 1 6 27 24 420 0.0 2 69 329 22 25 10403 0.0 2 9878 520 5 26 15 0.0 2 6 9 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 1015 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 3.7% C: 0.2% G: 79.3% T: 16.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 234.2 0 2 4 3 58.6 0 3 16 1 0.0 1 1 17 3 0.0 1 1 2 19 2 0.0 1 0 2 24 16 0.0 2 9 1 6 25 984 0.0 2 949 35 26 1 0.0 2 1 52 1 0.0 2 0 0 1 74 1 0.0 2 1 113 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 10958 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 48 234.2 0 48 4 7 58.6 0 7 5 4 14.6 0 4 17 1 0.0 1 0 1 21 1 0.0 2 1 22 4 0.0 2 1 0 3 23 20 0.0 2 1 4 15 24 324 0.0 2 51 264 9 25 10539 0.0 2 10239 288 12 26 9 0.0 2 2 7 34 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C36_19_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C36_211_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C36_19_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C36_211_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.53 s (137 us/read; 0.44 M reads/minute). === Summary === Total read pairs processed: 11,208 Read 1 with adapter: 9,781 (87.3%) Read 2 with adapter: 9,699 (86.5%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 11,208 (100.0%) Total basepairs processed: 5,427,408 bp Read 1: 2,716,939 bp Read 2: 2,710,469 bp Total written (filtered): 4,942,475 bp (91.1%) Read 1: 2,473,516 bp Read 2: 2,468,959 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 595 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 88.9% C: 3.2% G: 7.7% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 28 175.1 0 28 22 1 0.0 2 1 24 4 0.0 2 3 1 25 560 0.0 2 554 6 26 1 0.0 2 1 112 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 9186 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 7 175.1 0 7 20 2 0.0 2 1 0 1 21 1 0.0 2 1 22 1 0.0 2 0 1 23 28 0.0 2 3 7 18 24 358 0.0 2 60 288 10 25 8771 0.0 2 8334 425 12 26 18 0.0 2 9 8 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 606 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 8.3% C: 1.0% G: 87.3% T: 3.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 22 3 0.0 2 2 1 24 15 0.0 2 7 6 2 25 584 0.0 2 555 26 3 26 2 0.0 2 2 39 2 0.0 2 1 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 9093 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 26 175.1 0 26 4 4 43.8 0 4 5 1 10.9 0 1 19 1 0.0 1 0 1 22 3 0.0 2 1 1 1 23 22 0.0 2 2 4 16 24 255 0.0 2 63 188 4 25 8762 0.0 2 8514 246 2 26 19 0.0 2 10 9 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C37_20_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C37_212_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C37_20_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C37_212_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.65 s (147 us/read; 0.41 M reads/minute). === Summary === Total read pairs processed: 4,442 Read 1 with adapter: 3,767 (84.8%) Read 2 with adapter: 3,753 (84.5%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 4,442 (100.0%) Total basepairs processed: 2,113,608 bp Read 1: 1,056,216 bp Read 2: 1,057,392 bp Total written (filtered): 1,926,657 bp (91.2%) Read 1: 962,564 bp Read 2: 964,093 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 376 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 88.0% C: 5.6% G: 6.4% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 10 69.4 0 10 24 3 0.0 2 0 2 1 25 362 0.0 2 358 4 26 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 3391 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 5 69.4 0 5 4 1 17.4 0 1 19 1 0.0 1 0 1 22 1 0.0 2 1 23 14 0.0 2 3 2 9 24 138 0.0 2 17 116 5 25 3226 0.0 2 3085 135 6 26 5 0.0 2 0 5 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 320 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.0% C: 0.6% G: 80.0% T: 14.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 5 0.0 2 2 3 25 315 0.0 2 298 15 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 3433 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 13 69.4 0 13 4 6 17.4 0 6 20 1 0.0 2 0 1 22 1 0.0 2 1 23 5 0.0 2 0 1 4 24 94 0.0 2 12 79 3 25 3311 0.0 2 3217 91 3 26 1 0.0 2 0 1 27 1 0.0 2 0 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C4_21_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C4_213_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C4_21_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C4_213_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.50 s (149 us/read; 0.40 M reads/minute). === Summary === Total read pairs processed: 3,330 Read 1 with adapter: 3,063 (92.0%) Read 2 with adapter: 3,055 (91.7%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 3,330 (100.0%) Total basepairs processed: 1,533,072 bp Read 1: 766,591 bp Read 2: 766,481 bp Total written (filtered): 1,380,421 bp (90.0%) Read 1: 690,275 bp Read 2: 690,146 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 346 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 83.5% C: 11.6% G: 4.6% T: 0.3% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 4 52.0 0 4 24 3 0.0 2 3 25 338 0.0 2 334 4 26 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 2717 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 4 1 13.0 0 1 17 1 0.0 1 1 21 1 0.0 2 0 1 22 3 0.0 2 1 1 1 23 9 0.0 2 2 3 4 24 114 0.0 2 12 86 16 25 2583 0.0 2 2131 431 21 26 5 0.0 2 3 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 338 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.2% C: 0.9% G: 68.9% T: 21.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 22 1 0.0 2 0 1 24 8 0.0 2 2 4 2 25 327 0.0 2 296 27 4 26 1 0.0 2 1 113 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 2717 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 52.0 0 1 20 1 0.0 2 0 1 21 1 0.0 2 0 1 22 3 0.0 2 2 1 23 6 0.0 2 1 1 4 24 75 0.0 2 12 63 25 2622 0.0 2 2544 77 1 26 7 0.0 2 3 4 27 1 0.0 2 0 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C41_22_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C41_214_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C41_22_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C41_214_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.22 s (192 us/read; 0.31 M reads/minute). === Summary === Total read pairs processed: 1,137 Read 1 with adapter: 1,047 (92.1%) Read 2 with adapter: 1,039 (91.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,137 (100.0%) Total basepairs processed: 554,819 bp Read 1: 277,513 bp Read 2: 277,306 bp Total written (filtered): 502,824 bp (90.6%) Read 1: 251,442 bp Read 2: 251,382 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 43 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 83.7% C: 11.6% G: 4.7% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 2 17.8 0 2 25 41 0.0 2 40 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 1004 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 1 23 6 0.0 2 0 0 6 24 44 0.0 2 7 31 6 25 953 0.0 2 841 110 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 36 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 8.3% C: 0.0% G: 69.4% T: 22.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 0 0 1 24 1 0.0 2 0 0 1 25 34 0.0 2 33 0 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 1003 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 0 1 23 7 0.0 2 1 1 5 24 29 0.0 2 7 22 25 965 0.0 2 937 28 26 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C43_23_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C43_215_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C43_23_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C43_215_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (301 us/read; 0.20 M reads/minute). === Summary === Total read pairs processed: 93 Read 1 with adapter: 54 (58.1%) Read 2 with adapter: 53 (57.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 93 (100.0%) Total basepairs processed: 42,090 bp Read 1: 21,049 bp Read 2: 21,041 bp Total written (filtered): 39,445 bp (93.7%) Read 1: 19,707 bp Read 2: 19,738 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 20 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.0% C: 5.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 18 1 0.0 1 1 25 19 0.0 2 19 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 34 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 33 0.0 2 31 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 17 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 17 0.0 2 17 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 36 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1.5 0 1 25 35 0.0 2 35 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C44_24_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C44_216_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C44_24_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C44_216_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.24 s (165 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 1,424 Read 1 with adapter: 822 (57.7%) Read 2 with adapter: 816 (57.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,424 (100.0%) Total basepairs processed: 690,472 bp Read 1: 345,072 bp Read 2: 345,400 bp Total written (filtered): 649,973 bp (94.1%) Read 1: 324,783 bp Read 2: 325,190 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 47 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 63.8% C: 19.1% G: 14.9% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 22.2 0 5 4 1 5.6 0 1 25 41 0.0 2 39 1 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 775 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 22.2 0 4 23 3 0.0 2 0 1 2 24 36 0.0 2 8 25 3 25 732 0.0 2 699 32 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 48 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.7% C: 4.2% G: 64.6% T: 14.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 1 24 2 0.0 2 0 0 2 25 44 0.0 2 38 4 2 46 1 0.0 2 0 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 768 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 8 22.2 0 8 22 1 0.0 2 1 23 3 0.0 2 0 0 3 24 20 0.0 2 3 16 1 25 736 0.0 2 711 25 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C48_25_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C48_217_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C48_25_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C48_217_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.06 s (237 us/read; 0.25 M reads/minute). === Summary === Total read pairs processed: 256 Read 1 with adapter: 196 (76.6%) Read 2 with adapter: 196 (76.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 256 (100.0%) Total basepairs processed: 112,192 bp Read 1: 56,037 bp Read 2: 56,155 bp Total written (filtered): 102,450 bp (91.3%) Read 1: 51,149 bp Read 2: 51,301 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 52 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 88.5% C: 7.7% G: 1.9% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 18 1 0.0 1 1 24 1 0.0 2 0 1 25 50 0.0 2 50 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 144 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 5 0.0 2 1 4 25 138 0.0 2 133 5 26 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 51 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 2.0% C: 2.0% G: 84.3% T: 11.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 25 51 0.0 2 50 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 145 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 4.0 0 1 4 1 1.0 0 1 24 3 0.0 2 0 3 25 140 0.0 2 138 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C51_26_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C51_218_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C51_26_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C51_218_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.38 s (141 us/read; 0.43 M reads/minute). === Summary === Total read pairs processed: 2,690 Read 1 with adapter: 2,629 (97.7%) Read 2 with adapter: 2,631 (97.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 2,690 (100.0%) Total basepairs processed: 1,312,075 bp Read 1: 653,078 bp Read 2: 658,997 bp Total written (filtered): 1,180,802 bp (90.0%) Read 1: 587,501 bp Read 2: 593,301 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 104 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 96.2% C: 1.0% G: 1.0% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 42.0 0 1 25 103 0.0 2 103 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 2525 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 1 23 9 0.0 2 1 2 6 24 107 0.0 2 7 93 7 25 2405 0.0 2 2210 187 8 26 3 0.0 2 1 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 99 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.1% C: 1.0% G: 81.8% T: 11.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 97 0.0 2 93 3 1 53 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 2532 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 42.0 0 1 19 1 0.0 1 0 1 22 2 0.0 2 1 0 1 23 4 0.0 2 0 0 4 24 66 0.0 2 11 54 1 25 2456 0.0 2 2375 79 2 26 2 0.0 2 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C59_27_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C59_219_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C59_27_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C59_219_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.10 s (181 us/read; 0.33 M reads/minute). === Summary === Total read pairs processed: 560 Read 1 with adapter: 372 (66.4%) Read 2 with adapter: 357 (63.7%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 560 (100.0%) Total basepairs processed: 245,299 bp Read 1: 122,732 bp Read 2: 122,567 bp Total written (filtered): 227,333 bp (92.7%) Read 1: 113,586 bp Read 2: 113,747 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 170 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.3% C: 1.8% G: 2.9% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 5 8.8 0 5 24 3 0.0 2 0 3 25 162 0.0 2 162 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 202 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 4 1 2.2 0 1 19 1 0.0 1 0 1 24 15 0.0 2 0 15 25 184 0.0 2 176 7 1 26 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 166 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.6% C: 2.4% G: 94.6% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 5 0.0 2 2 3 25 160 0.0 2 157 2 1 39 1 0.0 2 0 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 191 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 5 8.8 0 5 24 4 0.0 2 0 4 25 182 0.0 2 179 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C6_28_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C6_220_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C6_28_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C6_220_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.42 s (142 us/read; 0.42 M reads/minute). === Summary === Total read pairs processed: 10,033 Read 1 with adapter: 9,722 (96.9%) Read 2 with adapter: 9,720 (96.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 10,033 (100.0%) Total basepairs processed: 4,969,609 bp Read 1: 2,482,076 bp Read 2: 2,487,533 bp Total written (filtered): 4,484,740 bp (90.2%) Read 1: 2,239,730 bp Read 2: 2,245,010 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 132 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 83.3% C: 4.5% G: 8.3% T: 3.8% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 9 156.8 0 9 24 1 0.0 2 1 25 122 0.0 2 121 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 9590 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 156.8 0 1 19 2 0.0 1 2 20 4 0.0 2 1 2 1 21 1 0.0 2 1 22 3 0.0 2 2 1 23 27 0.0 2 5 7 15 24 399 0.0 2 72 312 15 25 9138 0.0 2 8650 464 24 26 15 0.0 2 8 7 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 112 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.2% C: 0.0% G: 87.5% T: 6.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 0 1 25 110 0.0 2 104 5 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 9608 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 5 156.8 0 5 5 2 9.8 0 2 20 2 0.0 2 1 1 21 2 0.0 2 1 1 22 6 0.0 2 1 1 4 23 12 0.0 2 0 3 9 24 277 0.0 2 39 232 6 25 9291 0.0 2 9016 268 7 26 10 0.0 2 6 3 1 27 1 0.0 2 0 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C61_29_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C61_221_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C61_29_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C61_221_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.43 s (147 us/read; 0.41 M reads/minute). === Summary === Total read pairs processed: 9,750 Read 1 with adapter: 7,613 (78.1%) Read 2 with adapter: 7,559 (77.5%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 9,750 (100.0%) Total basepairs processed: 4,573,439 bp Read 1: 2,285,635 bp Read 2: 2,287,804 bp Total written (filtered): 4,196,890 bp (91.8%) Read 1: 2,096,779 bp Read 2: 2,100,111 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 931 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 79.3% C: 11.9% G: 8.4% T: 0.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 42 152.3 0 42 4 1 38.1 0 1 23 4 0.0 2 2 1 1 24 14 0.0 2 11 3 25 868 0.0 2 854 14 26 1 0.0 2 1 113 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 6682 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 10 152.3 0 10 4 3 38.1 0 3 11 1 0.0 1 0 1 21 1 0.0 2 0 0 1 22 3 0.0 2 1 1 1 23 13 0.0 2 1 3 9 24 263 0.0 2 27 203 33 25 6380 0.0 2 5435 917 28 26 8 0.0 2 3 5 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 831 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.9% C: 0.4% G: 79.9% T: 12.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 152.3 0 1 23 2 0.0 2 2 24 11 0.0 2 7 2 2 25 814 0.0 2 758 50 6 39 1 0.0 2 0 1 53 1 0.0 2 1 54 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 6728 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 42 152.3 0 42 4 9 38.1 0 9 22 1 0.0 2 0 0 1 23 17 0.0 2 1 2 14 24 183 0.0 2 37 141 5 25 6461 0.0 2 6275 183 3 26 13 0.0 2 2 11 27 2 0.0 2 0 0 2 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C66_30_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C66_222_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C66_30_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C66_222_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.23 s (143 us/read; 0.42 M reads/minute). === Summary === Total read pairs processed: 8,592 Read 1 with adapter: 5,859 (68.2%) Read 2 with adapter: 5,847 (68.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 8,592 (100.0%) Total basepairs processed: 4,244,938 bp Read 1: 2,121,679 bp Read 2: 2,123,259 bp Total written (filtered): 3,953,044 bp (93.1%) Read 1: 1,975,697 bp Read 2: 1,977,347 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 113 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.9% C: 12.4% G: 9.7% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 9 134.2 0 9 23 1 0.0 2 1 24 2 0.0 2 2 25 100 0.0 2 99 1 26 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 5746 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 134.2 0 1 19 1 0.0 1 0 1 20 1 0.0 2 0 0 1 22 4 0.0 2 3 1 23 15 0.0 2 1 1 13 24 239 0.0 2 34 185 20 25 5468 0.0 2 5023 426 19 26 16 0.0 2 11 5 31 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 99 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.1% C: 3.0% G: 70.7% T: 17.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 0 1 25 96 0.0 2 86 8 2 31 1 0.0 2 0 1 67 1 0.0 2 0 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 5748 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 134.2 0 3 4 1 33.6 0 1 22 4 0.0 2 3 1 23 16 0.0 2 1 3 12 24 192 0.0 2 37 150 5 25 5522 0.0 2 5340 180 2 26 9 0.0 2 4 5 29 1 0.0 2 0 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C70_31_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C70_223_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C70_31_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C70_223_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.32 s (143 us/read; 0.42 M reads/minute). === Summary === Total read pairs processed: 9,226 Read 1 with adapter: 8,987 (97.4%) Read 2 with adapter: 8,978 (97.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 9,226 (100.0%) Total basepairs processed: 4,579,791 bp Read 1: 2,290,219 bp Read 2: 2,289,572 bp Total written (filtered): 4,131,724 bp (90.2%) Read 1: 2,066,178 bp Read 2: 2,065,546 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 65 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 72.3% C: 15.4% G: 12.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6 144.2 0 6 25 59 0.0 2 56 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 8922 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 144.2 0 1 19 1 0.0 1 1 20 1 0.0 2 1 21 1 0.0 2 0 1 22 3 0.0 2 2 0 1 23 34 0.0 2 3 4 27 24 400 0.0 2 63 319 18 25 8469 0.0 2 8081 373 15 26 12 0.0 2 5 6 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 50 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.0% C: 2.0% G: 56.0% T: 26.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 23 2 0.0 2 0 1 1 25 48 0.0 2 42 5 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 8928 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 5 144.2 0 5 5 1 9.0 0 1 19 1 0.0 1 0 1 22 6 0.0 2 3 2 1 23 16 0.0 2 0 3 13 24 259 0.0 2 43 209 7 25 8632 0.0 2 8356 270 6 26 7 0.0 2 4 3 43 1 0.0 2 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C71_32_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C71_224_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C71_32_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C71_224_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.12 s (198 us/read; 0.30 M reads/minute). === Summary === Total read pairs processed: 593 Read 1 with adapter: 19 (3.2%) Read 2 with adapter: 17 (2.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 593 (100.0%) Total basepairs processed: 292,864 bp Read 1: 146,341 bp Read 2: 146,523 bp Total written (filtered): 292,248 bp (99.8%) Read 1: 146,018 bp Read 2: 146,230 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 5 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 9.3 0 4 25 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 14 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 9.3 0 2 5 1 0.6 0 1 25 11 0.0 2 7 3 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 4 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.0% C: 0.0% G: 25.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 4 0.0 2 1 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 13 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 6 9.3 0 6 25 7 0.0 2 5 2 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C72_33_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C72_225_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C72_33_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C72_225_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.25 s (163 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 1,553 Read 1 with adapter: 1,411 (90.9%) Read 2 with adapter: 1,410 (90.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,553 (100.0%) Total basepairs processed: 746,392 bp Read 1: 373,157 bp Read 2: 373,235 bp Total written (filtered): 676,088 bp (90.6%) Read 1: 337,984 bp Read 2: 338,104 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 96 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 82.3% C: 6.2% G: 8.3% T: 3.1% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 4 24.3 0 4 23 1 0.0 2 1 25 90 0.0 2 89 1 26 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 1315 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 24.3 0 1 19 1 0.0 1 1 23 3 0.0 2 1 1 1 24 57 0.0 2 6 39 12 25 1175 0.0 2 935 227 13 26 78 0.0 2 0 76 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 87 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 13.8% C: 0.0% G: 77.0% T: 9.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 3 0.0 2 2 0 1 25 84 0.0 2 73 8 3 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 1323 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 24.3 0 3 23 3 0.0 2 0 2 1 24 46 0.0 2 7 37 2 25 1269 0.0 2 1223 45 1 26 2 0.0 2 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C73_34_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C73_226_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C73_34_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C73_226_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.18 s (166 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 1,082 Read 1 with adapter: 652 (60.3%) Read 2 with adapter: 643 (59.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,082 (100.0%) Total basepairs processed: 472,689 bp Read 1: 236,170 bp Read 2: 236,519 bp Total written (filtered): 440,876 bp (93.3%) Read 1: 220,149 bp Read 2: 220,727 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 248 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 81.0% C: 10.9% G: 6.5% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 9 16.9 0 9 24 4 0.0 2 3 1 25 235 0.0 2 232 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 404 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 16.9 0 2 4 1 4.2 0 1 24 15 0.0 2 0 14 1 25 383 0.0 2 369 12 2 26 3 0.0 2 1 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 190 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 3.2% C: 0.0% G: 56.3% T: 40.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 16.9 0 1 24 2 0.0 2 0 1 1 25 183 0.0 2 177 5 1 26 1 0.0 2 1 28 3 0.0 2 3 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 453 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 11 16.9 0 11 4 1 4.2 0 1 24 8 0.0 2 3 5 25 431 0.0 2 420 11 26 2 0.0 2 0 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C74_35_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C74_227_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C74_35_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C74_227_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 2.45 s (176 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 13,906 Read 1 with adapter: 13,084 (94.1%) Read 2 with adapter: 13,098 (94.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 13,906 (100.0%) Total basepairs processed: 6,799,618 bp Read 1: 3,399,777 bp Read 2: 3,399,841 bp Total written (filtered): 6,146,936 bp (90.4%) Read 1: 3,073,677 bp Read 2: 3,073,259 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 513 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 92.0% C: 4.5% G: 2.1% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 11 217.3 0 11 24 2 0.0 2 1 1 25 500 0.0 2 489 11 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 12571 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 5 217.3 0 5 4 1 54.3 0 1 19 2 0.0 1 0 2 21 2 0.0 2 1 0 1 22 7 0.0 2 3 2 2 23 40 0.0 2 1 12 27 24 519 0.0 2 72 341 106 25 11980 0.0 2 8717 3164 99 26 15 0.0 2 9 6 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 477 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 7.3% C: 0.4% G: 88.1% T: 4.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 1 24 12 0.0 2 6 1 5 25 463 0.0 2 431 28 4 52 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 12621 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 21 217.3 0 21 4 1 54.3 0 1 5 1 13.6 0 1 19 1 0.0 1 1 20 1 0.0 2 1 22 2 0.0 2 0 1 1 23 20 0.0 2 3 2 15 24 366 0.0 2 71 283 12 25 12183 0.0 2 11801 372 10 26 16 0.0 2 11 5 27 8 0.0 2 0 0 8 38 1 0.0 2 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C75_36_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C75_228_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C75_36_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C75_228_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.12 s (210 us/read; 0.29 M reads/minute). === Summary === Total read pairs processed: 582 Read 1 with adapter: 366 (62.9%) Read 2 with adapter: 355 (61.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 582 (100.0%) Total basepairs processed: 254,021 bp Read 1: 127,015 bp Read 2: 127,006 bp Total written (filtered): 236,208 bp (93.0%) Read 1: 117,981 bp Read 2: 118,227 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 143 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 80.4% C: 3.5% G: 4.2% T: 11.9% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 4 9.1 0 4 24 2 0.0 2 1 1 25 137 0.0 2 134 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 223 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 9.1 0 1 24 5 0.0 2 0 5 25 216 0.0 2 198 16 2 26 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 144 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.4% C: 0.7% G: 66.7% T: 15.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 1 24 2 0.0 2 2 25 140 0.0 2 129 9 2 87 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 211 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 6 9.1 0 6 4 1 2.3 0 1 24 3 0.0 2 0 3 25 199 0.0 2 190 9 26 2 0.0 2 0 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C76_37_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C76_229_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C76_37_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C76_229_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.18 s (161 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 7,329 Read 1 with adapter: 6,847 (93.4%) Read 2 with adapter: 6,829 (93.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 7,329 (100.0%) Total basepairs processed: 3,550,568 bp Read 1: 1,775,549 bp Read 2: 1,775,019 bp Total written (filtered): 3,209,506 bp (90.4%) Read 1: 1,604,872 bp Read 2: 1,604,634 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 305 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 84.6% C: 6.9% G: 8.5% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 3 114.5 0 3 4 1 28.6 0 1 25 300 0.0 2 288 12 26 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 6542 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 114.5 0 2 5 1 7.2 0 1 19 1 0.0 1 1 21 3 0.0 2 2 0 1 22 5 0.0 2 2 1 2 23 36 0.0 2 3 13 20 24 252 0.0 2 28 216 8 25 6233 0.0 2 5993 235 5 26 9 0.0 2 2 7 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 290 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.6% C: 1.4% G: 75.5% T: 16.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 289 0.0 2 264 21 4 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 6539 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 6 114.5 0 6 21 1 0.0 2 1 22 3 0.0 2 0 1 2 23 10 0.0 2 0 3 7 24 199 0.0 2 31 165 3 25 6298 0.0 2 6120 174 4 26 19 0.0 2 4 15 27 3 0.0 2 0 0 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C77_38_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C77_230_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C77_38_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C77_230_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.43 s (172 us/read; 0.35 M reads/minute). === Summary === Total read pairs processed: 2,512 Read 1 with adapter: 2,008 (79.9%) Read 2 with adapter: 1,988 (79.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 2,512 (100.0%) Total basepairs processed: 1,152,541 bp Read 1: 576,400 bp Read 2: 576,141 bp Total written (filtered): 1,052,969 bp (91.4%) Read 1: 526,399 bp Read 2: 526,570 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 358 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.5% C: 3.1% G: 1.4% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 3 39.2 0 3 24 1 0.0 2 1 25 352 0.0 2 350 2 26 1 0.0 2 1 75 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 1650 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 39.2 0 4 4 1 9.8 0 1 19 1 0.0 1 1 23 5 0.0 2 0 1 4 24 69 0.0 2 11 51 7 25 1559 0.0 2 1366 184 9 26 11 0.0 2 0 11 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 352 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.5% C: 1.1% G: 84.9% T: 7.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 0 1 22 1 0.0 2 0 1 23 2 0.0 2 0 2 24 9 0.0 2 0 7 2 25 338 0.0 2 321 15 2 74 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 1636 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 39.2 0 2 4 3 9.8 0 3 22 1 0.0 2 0 1 23 3 0.0 2 0 2 1 24 52 0.0 2 14 37 1 25 1566 0.0 2 1516 50 26 8 0.0 2 4 4 27 1 0.0 2 0 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C79_39_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C79_231_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C79_39_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C79_231_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.18 s (182 us/read; 0.33 M reads/minute). === Summary === Total read pairs processed: 1,007 Read 1 with adapter: 725 (72.0%) Read 2 with adapter: 714 (70.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,007 (100.0%) Total basepairs processed: 475,376 bp Read 1: 237,755 bp Read 2: 237,621 bp Total written (filtered): 439,570 bp (92.5%) Read 1: 219,756 bp Read 2: 219,814 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 58 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 81.0% C: 10.3% G: 8.6% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 2 15.7 0 2 4 1 3.9 0 1 23 1 0.0 2 1 25 53 0.0 2 50 3 26 1 0.0 2 0 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 667 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 15.7 0 1 4 1 3.9 0 1 23 2 0.0 2 2 24 18 0.0 2 0 14 4 25 640 0.0 2 597 39 4 26 5 0.0 2 1 3 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 68 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.5% C: 8.8% G: 51.5% T: 16.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 0 1 25 67 0.0 2 55 10 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 646 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 15.7 0 1 23 4 0.0 2 1 1 2 24 14 0.0 2 1 13 25 625 0.0 2 601 24 26 2 0.0 2 0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C80_40_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C80_232_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C80_40_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C80_232_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.80 s (167 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 4,785 Read 1 with adapter: 4,593 (96.0%) Read 2 with adapter: 4,587 (95.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 4,785 (100.0%) Total basepairs processed: 2,362,194 bp Read 1: 1,181,429 bp Read 2: 1,180,765 bp Total written (filtered): 2,133,278 bp (90.3%) Read 1: 1,066,947 bp Read 2: 1,066,331 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 58 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 67.2% C: 24.1% G: 6.9% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 74.8 0 2 21 1 0.0 2 1 22 1 0.0 2 1 25 54 0.0 2 52 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 4535 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 74.8 0 1 4 1 18.7 0 1 18 1 0.0 1 1 21 1 0.0 2 1 23 19 0.0 2 2 4 13 24 207 0.0 2 29 160 18 25 4298 0.0 2 3950 342 6 26 7 0.0 2 1 5 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 54 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.5% C: 7.4% G: 42.6% T: 31.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 5 1 4.7 0 1 24 1 0.0 2 0 0 1 25 51 0.0 2 43 6 2 62 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 4533 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 5 74.8 0 5 4 1 18.7 0 1 17 1 0.0 1 0 1 22 1 0.0 2 1 23 7 0.0 2 1 2 4 24 113 0.0 2 12 97 4 25 4396 0.0 2 4264 126 6 26 8 0.0 2 4 4 29 1 0.0 2 0 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C84_41_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C84_233_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C84_41_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C84_233_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.91 s (149 us/read; 0.40 M reads/minute). === Summary === Total read pairs processed: 6,103 Read 1 with adapter: 4,875 (79.9%) Read 2 with adapter: 4,860 (79.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 6,103 (100.0%) Total basepairs processed: 2,947,663 bp Read 1: 1,473,166 bp Read 2: 1,474,497 bp Total written (filtered): 2,704,995 bp (91.8%) Read 1: 1,351,703 bp Read 2: 1,353,292 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 291 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 89.3% C: 5.5% G: 5.2% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 7 95.4 0 7 24 5 0.0 2 4 1 25 278 0.0 2 275 3 26 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 4584 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 1 23 25 0.0 2 4 5 16 24 206 0.0 2 24 179 3 25 4346 0.0 2 4144 200 2 26 6 0.0 2 4 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 282 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 8.9% C: 1.1% G: 79.8% T: 10.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 14 1 0.0 1 1 23 1 0.0 2 0 0 1 24 3 0.0 2 2 1 25 277 0.0 2 257 18 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 4578 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 5 95.4 0 5 4 1 23.8 0 1 22 3 0.0 2 2 0 1 23 10 0.0 2 1 1 8 24 129 0.0 2 20 106 3 25 4420 0.0 2 4299 118 3 26 10 0.0 2 5 4 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C85_42_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C85_234_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C85_42_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C85_234_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.10 s (178 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 568 Read 1 with adapter: 430 (75.7%) Read 2 with adapter: 426 (75.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 568 (100.0%) Total basepairs processed: 232,927 bp Read 1: 116,460 bp Read 2: 116,467 bp Total written (filtered): 211,713 bp (90.9%) Read 1: 105,769 bp Read 2: 105,944 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 156 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 90.4% C: 7.1% G: 2.6% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 155 0.0 2 154 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 274 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 8.9 0 1 4 1 2.2 0 1 19 1 0.0 1 0 1 24 11 0.0 2 1 8 2 25 259 0.0 2 237 19 3 27 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 157 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 7.6% C: 1.9% G: 79.0% T: 11.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 1 0.0 1 0 1 22 1 0.0 2 0 0 1 24 1 0.0 2 1 25 154 0.0 2 146 7 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 269 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 5 8.9 0 5 24 10 0.0 2 2 8 25 252 0.0 2 243 8 1 26 1 0.0 2 0 1 27 1 0.0 2 0 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C86_43_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C86_235_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C86_43_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C86_235_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.28 s (152 us/read; 0.40 M reads/minute). === Summary === Total read pairs processed: 1,819 Read 1 with adapter: 944 (51.9%) Read 2 with adapter: 889 (48.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,819 (100.0%) Total basepairs processed: 702,554 bp Read 1: 351,278 bp Read 2: 351,276 bp Total written (filtered): 657,457 bp (93.6%) Read 1: 327,989 bp Read 2: 329,468 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 714 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 91.2% C: 7.0% G: 1.8% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 11 28.4 0 11 5 1 1.8 0 1 20 2 0.0 2 2 22 1 0.0 2 1 23 3 0.0 2 3 24 5 0.0 2 2 3 25 690 0.0 2 685 5 113 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 230 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 28.4 0 3 4 2 7.1 0 2 24 8 0.0 2 1 4 3 25 214 0.0 2 170 39 5 26 3 0.0 2 1 1 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 672 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 8.8% C: 1.3% G: 79.8% T: 10.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 28.4 0 1 17 1 0.0 1 1 21 3 0.0 2 2 1 22 3 0.0 2 1 1 1 24 7 0.0 2 2 2 3 25 655 0.0 2 609 41 5 26 1 0.0 2 1 39 1 0.0 2 0 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 217 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 17 28.4 0 17 24 5 0.0 2 1 3 1 25 191 0.0 2 186 5 26 3 0.0 2 0 3 27 1 0.0 2 0 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C90_44_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C90_236_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C90_44_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C90_236_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 12.28 s (154 us/read; 0.39 M reads/minute). === Summary === Total read pairs processed: 79,818 Read 1 with adapter: 78,412 (98.2%) Read 2 with adapter: 78,518 (98.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 79,818 (100.0%) Total basepairs processed: 39,608,829 bp Read 1: 19,841,301 bp Read 2: 19,767,528 bp Total written (filtered): 35,693,187 bp (90.1%) Read 1: 17,885,359 bp Read 2: 17,807,828 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 481 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 79.0% C: 16.6% G: 3.7% T: 0.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 20 1247.2 0 20 4 1 311.8 0 1 18 1 0.0 1 1 24 7 0.0 2 1 6 25 451 0.0 2 444 7 26 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 77931 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 10 1247.2 0 10 4 4 311.8 0 4 14 1 0.0 1 1 15 1 0.0 1 0 1 16 2 0.0 1 0 2 18 1 0.0 1 0 1 19 14 0.0 1 6 6 2 20 4 0.0 2 2 1 1 21 10 0.0 2 2 5 3 22 20 0.0 2 8 6 6 23 241 0.0 2 26 60 155 24 3254 0.0 2 355 2030 869 25 73972 0.0 2 48957 24200 815 26 392 0.0 2 36 340 16 27 3 0.0 2 0 1 2 29 2 0.0 2 1 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 422 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 10.4% C: 2.4% G: 58.8% T: 28.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 1247.2 0 1 4 1 311.8 0 1 16 1 0.0 1 1 19 1 0.0 1 0 1 21 1 0.0 2 1 23 3 0.0 2 1 1 1 24 8 0.0 2 4 2 2 25 404 0.0 2 355 42 7 54 1 0.0 2 0 0 1 74 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 78096 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 20 1247.2 0 20 4 5 311.8 0 5 6 1 19.5 0 1 18 2 0.0 1 2 19 1 0.0 1 0 1 20 6 0.0 2 3 2 1 21 12 0.0 2 12 22 22 0.0 2 12 2 8 23 168 0.0 2 28 24 116 24 2298 0.0 2 340 1901 57 25 75467 0.0 2 72999 2417 51 26 91 0.0 2 55 33 3 27 1 0.0 2 0 1 28 1 0.0 2 0 1 39 1 0.0 2 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C92_45_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C92_237_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C92_45_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C92_237_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.24 s (153 us/read; 0.39 M reads/minute). === Summary === Total read pairs processed: 8,073 Read 1 with adapter: 4,345 (53.8%) Read 2 with adapter: 4,190 (51.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 8,073 (100.0%) Total basepairs processed: 3,544,413 bp Read 1: 1,771,804 bp Read 2: 1,772,609 bp Total written (filtered): 3,335,026 bp (94.1%) Read 1: 1,664,967 bp Read 2: 1,670,059 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 1736 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 91.9% C: 1.8% G: 6.2% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 56 126.1 0 56 4 1 31.5 0 1 6 1 2.0 0 1 23 1 0.0 2 0 0 1 24 14 0.0 2 8 5 1 25 1662 0.0 2 1650 11 1 26 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 2609 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 16 126.1 0 16 4 2 31.5 0 2 22 1 0.0 2 1 23 5 0.0 2 1 3 1 24 109 0.0 2 17 77 15 25 2461 0.0 2 2268 185 8 26 15 0.0 2 2 12 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 1882 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.6% C: 7.8% G: 84.7% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 4 2 31.5 0 2 6 1 2.0 0 1 20 1 0.0 2 1 21 1 0.0 2 1 22 1 0.0 2 1 23 2 0.0 2 1 1 24 40 0.0 2 18 13 9 25 1827 0.0 2 1710 108 9 26 2 0.0 2 1 1 46 1 0.0 2 0 0 1 51 1 0.0 2 0 1 53 1 0.0 2 0 1 68 1 0.0 2 1 95 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 2308 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 90 126.1 0 90 4 10 31.5 0 10 5 1 7.9 0 1 6 1 2.0 0 1 23 7 0.0 2 0 2 5 24 43 0.0 2 10 33 25 2143 0.0 2 2082 60 1 26 13 0.0 2 3 10 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C93_46_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C93_238_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C93_46_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C93_238_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.83 s (147 us/read; 0.41 M reads/minute). === Summary === Total read pairs processed: 5,661 Read 1 with adapter: 4,858 (85.8%) Read 2 with adapter: 4,761 (84.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 5,661 (100.0%) Total basepairs processed: 2,681,554 bp Read 1: 1,340,842 bp Read 2: 1,340,712 bp Total written (filtered): 2,442,225 bp (91.1%) Read 1: 1,219,966 bp Read 2: 1,222,259 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 337 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 81.0% C: 12.2% G: 6.5% T: 0.3% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 8 88.5 0 8 4 2 22.1 0 2 22 1 0.0 2 1 23 1 0.0 2 1 24 3 0.0 2 1 1 1 25 322 0.0 2 317 5 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 4521 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 88.5 0 4 4 1 22.1 0 1 5 1 5.5 0 1 20 1 0.0 2 0 1 22 1 0.0 2 1 23 10 0.0 2 1 2 7 24 206 0.0 2 28 130 48 25 4284 0.0 2 3551 702 31 26 11 0.0 2 5 4 2 27 2 0.0 2 0 0 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 408 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.1% C: 3.2% G: 50.2% T: 25.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 2 22.1 0 2 5 1 5.5 0 1 18 1 0.0 1 0 1 19 2 0.0 1 1 1 21 2 0.0 2 0 1 1 22 2 0.0 2 2 23 1 0.0 2 1 24 7 0.0 2 2 1 4 25 387 0.0 2 330 41 16 26 1 0.0 2 1 45 1 0.0 2 0 1 54 1 0.0 2 0 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 4353 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 14 88.5 0 14 4 3 22.1 0 3 21 1 0.0 2 0 1 22 2 0.0 2 1 0 1 23 6 0.0 2 2 0 4 24 131 0.0 2 19 108 4 25 4190 0.0 2 4057 133 26 6 0.0 2 2 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C95_47_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C95_239_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C95_47_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C95_239_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.19 s (137 us/read; 0.44 M reads/minute). === Summary === Total read pairs processed: 8,653 Read 1 with adapter: 8,447 (97.6%) Read 2 with adapter: 8,442 (97.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 8,653 (100.0%) Total basepairs processed: 4,285,930 bp Read 1: 2,142,754 bp Read 2: 2,143,176 bp Total written (filtered): 3,864,499 bp (90.2%) Read 1: 1,932,122 bp Read 2: 1,932,377 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 94 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 93.6% C: 1.1% G: 4.3% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 4 135.2 0 4 23 1 0.0 2 1 25 89 0.0 2 89 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 8353 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 135.2 0 1 14 1 0.0 1 1 19 2 0.0 1 2 21 1 0.0 2 0 1 22 6 0.0 2 0 0 6 23 34 0.0 2 4 7 23 24 357 0.0 2 53 292 12 25 7914 0.0 2 7511 387 16 26 36 0.0 2 5 31 28 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 97 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 7.2% C: 0.0% G: 74.2% T: 18.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 1 25 93 0.0 2 86 7 26 1 0.0 2 0 1 65 1 0.0 2 0 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 8345 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 18 1 0.0 1 0 1 19 1 0.0 1 1 22 2 0.0 2 2 23 18 0.0 2 4 2 12 24 246 0.0 2 43 195 8 25 8066 0.0 2 7831 233 2 26 11 0.0 2 6 5 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C96_48_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C96_240_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C96_48_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C96_240_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.07 s (214 us/read; 0.28 M reads/minute). === Summary === Total read pairs processed: 316 Read 1 with adapter: 153 (48.4%) Read 2 with adapter: 153 (48.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 316 (100.0%) Total basepairs processed: 150,701 bp Read 1: 75,315 bp Read 2: 75,386 bp Total written (filtered): 143,155 bp (95.0%) Read 1: 71,545 bp Read 2: 71,610 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 6 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.3% C: 50.0% G: 16.7% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 4.9 0 2 25 4 0.0 2 4 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 147 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 1 24 10 0.0 2 2 7 1 25 135 0.0 2 120 15 26 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 12 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 58.3% C: 0.0% G: 8.3% T: 33.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 4.9 0 2 24 2 0.0 2 1 0 1 25 8 0.0 2 4 4 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 141 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 0 1 24 1 0.0 2 0 1 25 139 0.0 2 136 3 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C97_49_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C97_241_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C97_49_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C97_241_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 2.55 s (162 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 15,805 Read 1 with adapter: 14,778 (93.5%) Read 2 with adapter: 14,802 (93.7%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 15,805 (100.0%) Total basepairs processed: 7,799,106 bp Read 1: 3,901,436 bp Read 2: 3,897,670 bp Total written (filtered): 7,060,948 bp (90.5%) Read 1: 3,532,762 bp Read 2: 3,528,186 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 233 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 85.0% C: 10.7% G: 4.3% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 3 247.0 0 3 4 1 61.7 0 1 24 2 0.0 2 0 2 25 227 0.0 2 223 4 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 14545 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 247.0 0 2 18 1 0.0 1 1 19 2 0.0 1 0 2 20 1 0.0 2 0 1 21 2 0.0 2 1 1 22 5 0.0 2 3 2 23 44 0.0 2 7 5 32 24 612 0.0 2 57 330 225 25 13773 0.0 2 7707 5882 184 26 102 0.0 2 7 88 7 27 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 211 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 7.6% C: 0.5% G: 67.8% T: 24.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 1 24 4 0.0 2 1 1 2 25 206 0.0 2 191 14 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 14591 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 247.0 0 2 5 1 15.4 0 1 18 1 0.0 1 1 21 3 0.0 2 1 1 1 22 2 0.0 2 1 0 1 23 37 0.0 2 4 7 26 24 429 0.0 2 67 354 8 25 14088 0.0 2 13660 412 16 26 25 0.0 2 16 8 1 27 2 0.0 2 1 0 1 28 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep1_50_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep1_242_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep1_50_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep1_242_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (287 us/read; 0.21 M reads/minute). === Summary === Total read pairs processed: 112 Read 1 with adapter: 104 (92.9%) Read 2 with adapter: 103 (92.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 112 (100.0%) Total basepairs processed: 55,108 bp Read 1: 27,545 bp Read 2: 27,563 bp Total written (filtered): 49,966 bp (90.7%) Read 1: 24,973 bp Read 2: 24,993 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 3 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 3 0.0 2 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 101 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1.8 0 1 24 6 0.0 2 2 4 25 94 0.0 2 89 5 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 3 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 3 0.0 2 3 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 100 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 0 1 24 3 0.0 2 1 2 25 96 0.0 2 92 4 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep10_51_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep10_243_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep10_51_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep10_243_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.04 s (293 us/read; 0.21 M reads/minute). === Summary === Total read pairs processed: 132 Read 1 with adapter: 118 (89.4%) Read 2 with adapter: 117 (88.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 132 (100.0%) Total basepairs processed: 63,216 bp Read 1: 31,608 bp Read 2: 31,608 bp Total written (filtered): 57,377 bp (90.8%) Read 1: 28,686 bp Read 2: 28,691 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 12 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 83.3% C: 0.0% G: 16.7% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 12 0.0 2 12 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 106 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 2.1 0 1 23 1 0.0 2 0 0 1 24 4 0.0 2 0 4 25 100 0.0 2 94 6 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 9 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 8 0.0 2 8 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 108 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 1 24 5 0.0 2 0 5 25 102 0.0 2 100 2 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep105_52_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep105_244_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep105_52_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep105_244_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.09 s (222 us/read; 0.27 M reads/minute). === Summary === Total read pairs processed: 395 Read 1 with adapter: 333 (84.3%) Read 2 with adapter: 338 (85.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 395 (100.0%) Total basepairs processed: 188,977 bp Read 1: 94,529 bp Read 2: 94,448 bp Total written (filtered): 172,371 bp (91.2%) Read 1: 86,232 bp Read 2: 86,139 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 16 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 68.8% C: 18.8% G: 6.2% T: 6.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 6.2 0 1 25 15 0.0 2 14 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 317 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 6 0.0 2 1 5 25 311 0.0 2 296 15 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 16 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.8% C: 0.0% G: 68.8% T: 12.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 16 0.0 2 13 3 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 322 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 5 6.2 0 5 5 1 0.4 0 1 23 1 0.0 2 0 0 1 24 9 0.0 2 1 6 2 25 306 0.0 2 294 12 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep110_53_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep110_245_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep110_53_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep110_245_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.08 s (213 us/read; 0.28 M reads/minute). === Summary === Total read pairs processed: 360 Read 1 with adapter: 335 (93.1%) Read 2 with adapter: 334 (92.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 360 (100.0%) Total basepairs processed: 170,871 bp Read 1: 85,397 bp Read 2: 85,474 bp Total written (filtered): 154,203 bp (90.2%) Read 1: 77,062 bp Read 2: 77,141 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 9 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.7% C: 11.1% G: 22.2% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 9 0.0 2 9 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 326 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 5.6 0 1 23 2 0.0 2 0 0 2 24 15 0.0 2 1 11 3 25 307 0.0 2 293 10 4 26 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 20 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.0% C: 0.0% G: 15.0% T: 30.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 3 0.0 2 0 0 3 25 17 0.0 2 8 5 4 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 314 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 22 1 0.0 2 0 0 1 23 1 0.0 2 0 0 1 24 9 0.0 2 0 9 25 303 0.0 2 295 8 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep12_54_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep12_246_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep12_54_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep12_246_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.11 s (214 us/read; 0.28 M reads/minute). === Summary === Total read pairs processed: 501 Read 1 with adapter: 386 (77.0%) Read 2 with adapter: 381 (76.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 501 (100.0%) Total basepairs processed: 222,228 bp Read 1: 111,088 bp Read 2: 111,140 bp Total written (filtered): 203,172 bp (91.4%) Read 1: 101,523 bp Read 2: 101,649 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 54 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 59.3% C: 20.4% G: 14.8% T: 5.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 7.8 0 2 25 52 0.0 2 50 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 332 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 4 1 2.0 0 1 23 1 0.0 2 1 24 20 0.0 2 2 18 25 308 0.0 2 281 27 26 2 0.0 2 0 1 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 63 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.8% C: 19.0% G: 49.2% T: 27.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 63 0.0 2 49 14 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 318 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 7.8 0 1 18 1 0.0 1 1 24 6 0.0 2 1 5 25 309 0.0 2 299 10 26 1 0.0 2 0 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep14_55_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep14_247_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep14_55_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep14_247_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.12 s (176 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 676 Read 1 with adapter: 637 (94.2%) Read 2 with adapter: 636 (94.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 676 (100.0%) Total basepairs processed: 311,840 bp Read 1: 155,700 bp Read 2: 156,140 bp Total written (filtered): 280,098 bp (89.8%) Read 1: 139,841 bp Read 2: 140,257 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 51 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 72.5% C: 19.6% G: 7.8% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 10.6 0 2 25 49 0.0 2 49 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 586 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 0 1 24 18 0.0 2 9 9 25 567 0.0 2 526 41 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 56 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.9% C: 1.8% G: 46.4% T: 33.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 56 0.0 2 45 11 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 580 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 0 1 24 16 0.0 2 4 12 25 562 0.0 2 543 18 1 26 1 0.0 2 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep16_56_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep16_248_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep16_56_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep16_248_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.05 s (247 us/read; 0.24 M reads/minute). === Summary === Total read pairs processed: 199 Read 1 with adapter: 22 (11.1%) Read 2 with adapter: 19 (9.5%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 199 (100.0%) Total basepairs processed: 94,385 bp Read 1: 47,156 bp Read 2: 47,229 bp Total written (filtered): 93,427 bp (99.0%) Read 1: 46,672 bp Read 2: 46,755 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 12 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.0% C: 50.0% G: 25.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 3.1 0 2 4 1 0.8 0 1 25 9 0.0 2 8 0 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 10 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 9 0.0 2 7 1 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 5 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.0% C: 0.0% G: 40.0% T: 40.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 5 0.0 2 4 0 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 14 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 0 1 25 12 0.0 2 11 1 26 1 0.0 2 0 0 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep19_57_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep19_249_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep19_57_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep19_249_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.02 s (434 us/read; 0.14 M reads/minute). === Summary === Total read pairs processed: 50 Read 1 with adapter: 28 (56.0%) Read 2 with adapter: 27 (54.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 50 (100.0%) Total basepairs processed: 18,223 bp Read 1: 9,113 bp Read 2: 9,110 bp Total written (filtered): 16,919 bp (92.8%) Read 1: 8,457 bp Read 2: 8,462 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 12 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.0% C: 16.7% G: 8.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 0.8 0 1 25 11 0.0 2 11 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 16 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 0.8 0 1 25 15 0.0 2 12 3 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 13 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.1% C: 0.0% G: 15.4% T: 61.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 0.8 0 1 25 12 0.0 2 9 3 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 14 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 0 1 24 3 0.0 2 1 2 25 10 0.0 2 10 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep20_58_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep20_250_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep20_58_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep20_250_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (227 us/read; 0.26 M reads/minute). === Summary === Total read pairs processed: 136 Read 1 with adapter: 84 (61.8%) Read 2 with adapter: 84 (61.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 136 (100.0%) Total basepairs processed: 65,439 bp Read 1: 32,688 bp Read 2: 32,751 bp Total written (filtered): 61,246 bp (93.6%) Read 1: 30,590 bp Read 2: 30,656 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 0 times === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 84 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 0 1 25 82 0.0 2 78 4 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 4 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 0 1 25 3 0.0 2 0 3 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 80 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 4 0.0 2 0 4 25 76 0.0 2 75 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep22_59_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep22_251_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep22_59_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep22_251_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.54 s (137 us/read; 0.44 M reads/minute). === Summary === Total read pairs processed: 3,929 Read 1 with adapter: 3,639 (92.6%) Read 2 with adapter: 3,640 (92.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 3,929 (100.0%) Total basepairs processed: 1,759,145 bp Read 1: 879,558 bp Read 2: 879,587 bp Total written (filtered): 1,577,700 bp (89.7%) Read 1: 788,912 bp Read 2: 788,788 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 241 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.3% C: 15.8% G: 7.5% T: 0.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 61.4 0 5 4 1 15.3 0 1 23 1 0.0 2 0 0 1 24 3 0.0 2 2 1 25 231 0.0 2 228 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 3398 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 61.4 0 2 21 2 0.0 2 1 1 23 11 0.0 2 1 3 7 24 126 0.0 2 19 80 27 25 3250 0.0 2 2454 758 38 26 7 0.0 2 4 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 320 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.7% C: 0.3% G: 8.4% T: 61.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 6 1 1.0 0 1 24 12 0.0 2 2 1 9 25 307 0.0 2 215 75 17 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 3320 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 61.4 0 2 21 2 0.0 2 2 23 7 0.0 2 0 3 4 24 112 0.0 2 15 95 2 25 3192 0.0 2 3104 87 1 26 4 0.0 2 2 2 29 1 0.0 2 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep23_60_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep23_252_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep23_60_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep23_252_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.02 s (730 us/read; 0.08 M reads/minute). === Summary === Total read pairs processed: 32 Read 1 with adapter: 22 (68.8%) Read 2 with adapter: 22 (68.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 32 (100.0%) Total basepairs processed: 10,323 bp Read 1: 5,161 bp Read 2: 5,162 bp Total written (filtered): 9,224 bp (89.4%) Read 1: 4,611 bp Read 2: 4,613 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 8 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 62.5% C: 37.5% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 8 0.0 2 8 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 14 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 25 14 0.0 2 11 3 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 9 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 22.2% C: 0.0% G: 22.2% T: 55.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 9 0.0 2 7 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 13 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 12 0.0 2 12 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep30_61_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep30_253_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep30_61_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep30_253_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (311 us/read; 0.19 M reads/minute). === Summary === Total read pairs processed: 86 Read 1 with adapter: 69 (80.2%) Read 2 with adapter: 70 (81.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 86 (100.0%) Total basepairs processed: 34,899 bp Read 1: 17,412 bp Read 2: 17,487 bp Total written (filtered): 31,429 bp (90.1%) Read 1: 15,689 bp Read 2: 15,740 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 15 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.0% C: 33.3% G: 6.7% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 15 0.0 2 15 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 54 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 3 0.0 2 1 2 25 50 0.0 2 44 6 26 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 15 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.0% C: 0.0% G: 53.3% T: 26.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 13 0.0 2 10 3 26 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 55 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 3 0.0 2 0 2 1 25 52 0.0 2 52 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep31_62_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep31_254_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep31_62_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep31_254_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.06 s (232 us/read; 0.26 M reads/minute). === Summary === Total read pairs processed: 251 Read 1 with adapter: 140 (55.8%) Read 2 with adapter: 141 (56.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 251 (100.0%) Total basepairs processed: 121,086 bp Read 1: 60,542 bp Read 2: 60,544 bp Total written (filtered): 114,118 bp (94.2%) Read 1: 57,072 bp Read 2: 57,046 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 11 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 0 1 25 10 0.0 2 10 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 129 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 3.9 0 1 23 1 0.0 2 0 0 1 24 5 0.0 2 2 3 25 122 0.0 2 115 7 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 14 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.3% C: 0.0% G: 64.3% T: 21.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 0 1 25 13 0.0 2 12 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 127 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 3.9 0 1 24 3 0.0 2 0 3 25 123 0.0 2 117 6 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep33_63_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep33_255_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep33_63_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep33_255_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 2.83 s (168 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 16,818 Read 1 with adapter: 4,730 (28.1%) Read 2 with adapter: 4,701 (28.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 16,818 (100.0%) Total basepairs processed: 8,222,974 bp Read 1: 4,107,950 bp Read 2: 4,115,024 bp Total written (filtered): 7,987,982 bp (97.1%) Read 1: 3,990,463 bp Read 2: 3,997,519 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 639 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.3% C: 1.6% G: 3.0% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 24 262.8 0 24 4 2 65.7 0 2 24 3 0.0 2 3 25 608 0.0 2 603 5 75 1 0.0 2 1 76 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 4091 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 262.8 0 2 4 3 65.7 0 3 21 1 0.0 2 0 0 1 22 1 0.0 2 0 1 23 13 0.0 2 1 2 10 24 165 0.0 2 13 143 9 25 3892 0.0 2 3717 169 6 26 14 0.0 2 3 11 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 640 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.2% C: 2.0% G: 90.2% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 2 262.8 0 2 4 3 65.7 0 3 24 13 0.0 2 5 5 3 25 617 0.0 2 567 46 4 26 1 0.0 2 1 30 1 0.0 2 1 92 1 0.0 2 0 1 113 2 0.0 2 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 4061 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 262.8 0 2 4 1 65.7 0 1 23 11 0.0 2 1 2 8 24 114 0.0 2 24 85 5 25 3921 0.0 2 3798 122 1 26 7 0.0 2 3 4 34 5 0.0 2 4 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep34_64_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep34_256_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep34_64_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep34_256_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.08 s (186 us/read; 0.32 M reads/minute). === Summary === Total read pairs processed: 434 Read 1 with adapter: 417 (96.1%) Read 2 with adapter: 417 (96.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 434 (100.0%) Total basepairs processed: 195,611 bp Read 1: 97,926 bp Read 2: 97,685 bp Total written (filtered): 174,784 bp (89.4%) Read 1: 87,516 bp Read 2: 87,268 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 48 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 68.8% C: 16.7% G: 14.6% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 47 0.0 2 46 1 26 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 369 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 1 24 14 0.0 2 1 12 1 25 354 0.0 2 344 10 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 45 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.1% C: 0.0% G: 64.4% T: 24.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 44 0.0 2 38 6 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 372 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 9 0.0 2 4 4 1 25 361 0.0 2 347 14 26 2 0.0 2 1 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep35_65_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep35_257_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep35_65_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep35_257_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (370 us/read; 0.16 M reads/minute). === Summary === Total read pairs processed: 71 Read 1 with adapter: 16 (22.5%) Read 2 with adapter: 16 (22.5%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 71 (100.0%) Total basepairs processed: 33,455 bp Read 1: 16,723 bp Read 2: 16,732 bp Total written (filtered): 32,743 bp (97.9%) Read 1: 16,367 bp Read 2: 16,376 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 2 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 0.0% G: 50.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 1.1 0 1 25 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 14 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1.1 0 1 25 13 0.0 2 10 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 4 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.0% C: 0.0% G: 0.0% T: 25.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 4 0.0 2 1 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 12 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 1.1 0 2 25 10 0.0 2 10 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep36_66_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep36_258_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep36_66_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep36_258_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.02 s (638 us/read; 0.09 M reads/minute). === Summary === Total read pairs processed: 36 Read 1 with adapter: 27 (75.0%) Read 2 with adapter: 27 (75.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 36 (100.0%) Total basepairs processed: 12,450 bp Read 1: 6,225 bp Read 2: 6,225 bp Total written (filtered): 11,100 bp (89.2%) Read 1: 5,550 bp Read 2: 5,550 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 4 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.0% C: 50.0% G: 0.0% T: 25.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 4 0.0 2 3 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 23 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 25 23 0.0 2 16 6 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 7 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 85.7% C: 0.0% G: 0.0% T: 14.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 7 0.0 2 1 5 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 20 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 1 25 17 0.0 2 16 1 27 1 0.0 2 0 0 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep4_67_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep4_259_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep4_67_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep4_259_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.02 s (524 us/read; 0.11 M reads/minute). === Summary === Total read pairs processed: 33 Read 1 with adapter: 13 (39.4%) Read 2 with adapter: 13 (39.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 33 (100.0%) Total basepairs processed: 9,241 bp Read 1: 4,621 bp Read 2: 4,620 bp Total written (filtered): 8,591 bp (93.0%) Read 1: 4,296 bp Read 2: 4,295 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 3 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.3% C: 33.3% G: 33.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 3 0.0 2 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 10 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 25 10 0.0 2 2 5 3 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 11 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 63.6% C: 0.0% G: 27.3% T: 9.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 11 0.0 2 3 5 3 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 2 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 25 2 0.0 2 2 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep41_68_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep41_260_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep41_68_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep41_260_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (233 us/read; 0.26 M reads/minute). === Summary === Total read pairs processed: 147 Read 1 with adapter: 133 (90.5%) Read 2 with adapter: 132 (89.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 147 (100.0%) Total basepairs processed: 63,481 bp Read 1: 31,720 bp Read 2: 31,761 bp Total written (filtered): 56,888 bp (89.6%) Read 1: 28,419 bp Read 2: 28,469 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 26 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 92.3% C: 7.7% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 5 1 0.1 0 1 25 25 0.0 2 25 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 107 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 4 0.0 2 0 4 25 103 0.0 2 98 3 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 28 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 10.7% C: 0.0% G: 67.9% T: 21.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 28 0.0 2 24 2 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 104 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 1 24 4 0.0 2 1 3 25 99 0.0 2 95 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep44_69_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep44_261_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep44_69_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep44_261_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.04 s (343 us/read; 0.17 M reads/minute). === Summary === Total read pairs processed: 118 Read 1 with adapter: 45 (38.1%) Read 2 with adapter: 45 (38.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 118 (100.0%) Total basepairs processed: 57,197 bp Read 1: 28,574 bp Read 2: 28,623 bp Total written (filtered): 54,952 bp (96.1%) Read 1: 27,451 bp Read 2: 27,501 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 2 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 2 0.0 2 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 43 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 1 25 41 0.0 2 38 3 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 2 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 0.0% T: 100.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 2 0.0 2 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 43 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 1 24 1 0.0 2 0 1 25 41 0.0 2 39 2 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep48_70_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep48_262_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep48_70_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep48_262_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.02 s (2073 us/read; 0.03 M reads/minute). === Summary === Total read pairs processed: 10 Read 1 with adapter: 9 (90.0%) Read 2 with adapter: 9 (90.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 10 (100.0%) Total basepairs processed: 4,981 bp Read 1: 2,492 bp Read 2: 2,489 bp Total written (filtered): 4,531 bp (91.0%) Read 1: 2,267 bp Read 2: 2,264 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 2 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 2 0.0 2 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 7 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 25 7 0.0 2 6 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 2 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 2 0.0 2 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 7 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 25 7 0.0 2 7 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep51_71_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep51_263_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep51_71_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep51_263_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (431 us/read; 0.14 M reads/minute). === Summary === Total read pairs processed: 70 Read 1 with adapter: 48 (68.6%) Read 2 with adapter: 46 (65.7%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 70 (100.0%) Total basepairs processed: 25,819 bp Read 1: 12,909 bp Read 2: 12,910 bp Total written (filtered): 23,557 bp (91.2%) Read 1: 11,754 bp Read 2: 11,803 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 11 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 36.4% C: 18.2% G: 36.4% T: 9.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 11 0.0 2 9 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 37 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 1.1 0 2 24 1 0.0 2 0 1 25 34 0.0 2 30 3 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 15 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.7% C: 0.0% G: 66.7% T: 6.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 1.1 0 2 25 13 0.0 2 9 3 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 31 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 25 30 0.0 2 30 26 1 0.0 2 0 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep59_72_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep59_264_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep59_72_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep59_264_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.33 s (152 us/read; 0.39 M reads/minute). === Summary === Total read pairs processed: 8,710 Read 1 with adapter: 8,639 (99.2%) Read 2 with adapter: 8,638 (99.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 8,710 (100.0%) Total basepairs processed: 4,321,219 bp Read 1: 2,160,177 bp Read 2: 2,161,042 bp Total written (filtered): 3,890,146 bp (90.0%) Read 1: 1,944,706 bp Read 2: 1,945,440 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 100 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 88.0% C: 10.0% G: 1.0% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 4 1 34.0 0 1 25 99 0.0 2 98 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 8539 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 136.1 0 1 16 1 0.0 1 0 1 19 2 0.0 1 2 20 1 0.0 2 1 21 1 0.0 2 1 22 2 0.0 2 0 1 1 23 38 0.0 2 5 9 24 24 359 0.0 2 55 293 11 25 8124 0.0 2 7776 342 6 26 10 0.0 2 5 5 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 109 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.5% C: 2.8% G: 76.1% T: 15.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 136.1 0 1 24 2 0.0 2 1 0 1 25 105 0.0 2 94 8 3 26 1 0.0 2 0 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 8529 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 4 1 34.0 0 1 21 1 0.0 2 1 22 1 0.0 2 0 1 23 25 0.0 2 1 5 19 24 259 0.0 2 43 206 10 25 8230 0.0 2 7961 264 5 26 12 0.0 2 9 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep6_73_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep6_265_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep6_73_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep6_265_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (310 us/read; 0.19 M reads/minute). === Summary === Total read pairs processed: 100 Read 1 with adapter: 59 (59.0%) Read 2 with adapter: 60 (60.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 100 (100.0%) Total basepairs processed: 29,691 bp Read 1: 14,843 bp Read 2: 14,848 bp Total written (filtered): 26,785 bp (90.2%) Read 1: 13,392 bp Read 2: 13,393 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 36 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.8% C: 13.9% G: 5.6% T: 2.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 1 0.4 0 1 25 35 0.0 2 35 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 23 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 3 0.0 2 1 1 1 25 20 0.0 2 13 6 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 38 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.1% C: 0.0% G: 5.3% T: 73.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 0 1 25 37 0.0 2 30 6 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 22 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1.6 0 1 4 1 0.4 0 1 24 1 0.0 2 0 1 25 19 0.0 2 19 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep61_74_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep61_266_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep61_74_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep61_266_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.06 s (228 us/read; 0.26 M reads/minute). === Summary === Total read pairs processed: 251 Read 1 with adapter: 191 (76.1%) Read 2 with adapter: 192 (76.5%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 251 (100.0%) Total basepairs processed: 112,495 bp Read 1: 56,229 bp Read 2: 56,266 bp Total written (filtered): 102,937 bp (91.5%) Read 1: 51,461 bp Read 2: 51,476 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 32 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.1% C: 18.8% G: 3.1% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 31 0.0 2 30 0 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 159 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 0 1 24 4 0.0 2 1 3 25 154 0.0 2 149 5 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 23 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.3% C: 0.0% G: 39.1% T: 56.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 20 1 0.0 2 1 25 22 0.0 2 20 1 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 169 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 5 0.0 2 0 5 25 164 0.0 2 160 3 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep74_75_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep74_267_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep74_75_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep74_267_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.10 s (171 us/read; 0.35 M reads/minute). === Summary === Total read pairs processed: 586 Read 1 with adapter: 571 (97.4%) Read 2 with adapter: 571 (97.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 586 (100.0%) Total basepairs processed: 288,699 bp Read 1: 144,382 bp Read 2: 144,317 bp Total written (filtered): 260,367 bp (90.2%) Read 1: 130,285 bp Read 2: 130,082 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 35 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 97.1% C: 0.0% G: 2.9% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 20 29 0.0 2 29 22 1 0.0 2 1 23 1 0.0 2 1 25 4 0.0 2 4 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 536 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 5 0.0 2 2 1 2 24 19 0.0 2 0 19 25 511 0.0 2 490 20 1 26 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 11 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.3% C: 36.4% G: 18.2% T: 18.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 1 0.0 1 1 20 3 0.0 2 2 1 25 7 0.0 2 4 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 560 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 19 0.0 2 2 16 1 25 541 0.0 2 522 19 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep75_76_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep75_268_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep75_76_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep75_268_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 14.11 s (126 us/read; 0.48 M reads/minute). === Summary === Total read pairs processed: 112,412 Read 1 with adapter: 112,183 (99.8%) Read 2 with adapter: 112,232 (99.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 112,412 (100.0%) Total basepairs processed: 56,112,793 bp Read 1: 28,058,363 bp Read 2: 28,054,430 bp Total written (filtered): 50,512,391 bp (90.0%) Read 1: 25,259,711 bp Read 2: 25,252,680 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 251 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 88.4% C: 8.4% G: 2.0% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 3 1756.4 0 3 24 2 0.0 2 1 1 25 246 0.0 2 241 5 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 111932 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 1756.4 0 2 11 1 0.0 1 1 12 1 0.0 1 1 15 1 0.0 1 1 17 3 0.0 1 1 2 18 3 0.0 1 2 1 19 11 0.0 1 7 4 20 7 0.0 2 3 4 21 19 0.0 2 13 4 2 22 54 0.0 2 16 13 25 23 402 0.0 2 32 91 279 24 4732 0.0 2 737 3870 125 25 106570 0.0 2 102641 3844 85 26 123 0.0 2 71 51 1 29 2 0.0 2 2 40 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 232 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.3% C: 0.4% G: 75.0% T: 20.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 1756.4 0 1 16 1 0.0 1 1 24 1 0.0 2 0 0 1 25 228 0.0 2 218 8 2 27 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 112000 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1756.4 0 1 4 1 439.1 0 1 5 1 109.8 0 1 17 1 0.0 1 1 18 4 0.0 1 3 1 19 2 0.0 1 2 20 8 0.0 2 4 3 1 21 21 0.0 2 12 5 4 22 31 0.0 2 19 4 8 23 247 0.0 2 34 39 174 24 3346 0.0 2 561 2698 87 25 108207 0.0 2 104942 3200 65 26 125 0.0 2 70 53 2 27 1 0.0 2 1 28 2 0.0 2 2 29 1 0.0 2 0 1 36 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep77_77_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep77_269_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep77_77_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep77_269_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.11 s (195 us/read; 0.31 M reads/minute). === Summary === Total read pairs processed: 554 Read 1 with adapter: 515 (93.0%) Read 2 with adapter: 517 (93.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 554 (100.0%) Total basepairs processed: 270,088 bp Read 1: 134,897 bp Read 2: 135,191 bp Total written (filtered): 244,356 bp (90.5%) Read 1: 122,052 bp Read 2: 122,304 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 19 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.9% C: 15.8% G: 5.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 19 0.0 2 19 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 496 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 3 0.0 2 0 0 3 24 26 0.0 2 7 18 1 25 465 0.0 2 452 13 26 2 0.0 2 0 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 16 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 0 2 25 14 0.0 2 14 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 501 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 4 1 2.2 0 1 23 1 0.0 2 0 0 1 24 13 0.0 2 1 12 25 486 0.0 2 470 16 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep79_78_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep79_270_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep79_78_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep79_270_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.05 s (278 us/read; 0.22 M reads/minute). === Summary === Total read pairs processed: 181 Read 1 with adapter: 42 (23.2%) Read 2 with adapter: 42 (23.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 181 (100.0%) Total basepairs processed: 89,158 bp Read 1: 44,558 bp Read 2: 44,600 bp Total written (filtered): 87,062 bp (97.6%) Read 1: 43,511 bp Read 2: 43,551 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 1 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 41 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 0 2 25 39 0.0 2 35 4 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 2 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 50.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 2 0.0 2 1 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 40 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 39 0.0 2 38 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep80_79_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep80_271_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep80_79_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep80_271_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (467 us/read; 0.13 M reads/minute). === Summary === Total read pairs processed: 54 Read 1 with adapter: 11 (20.4%) Read 2 with adapter: 11 (20.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 54 (100.0%) Total basepairs processed: 26,161 bp Read 1: 13,077 bp Read 2: 13,084 bp Total written (filtered): 25,613 bp (97.9%) Read 1: 12,803 bp Read 2: 12,810 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 1 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 10 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 9 0.0 2 9 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 0 times === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 11 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 10 0.0 2 10 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep84_80_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep84_272_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep84_80_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep84_272_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (460 us/read; 0.13 M reads/minute). === Summary === Total read pairs processed: 64 Read 1 with adapter: 45 (70.3%) Read 2 with adapter: 46 (71.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 64 (100.0%) Total basepairs processed: 23,398 bp Read 1: 11,705 bp Read 2: 11,693 bp Total written (filtered): 21,148 bp (90.4%) Read 1: 10,583 bp Read 2: 10,565 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 7 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.9% C: 42.9% G: 14.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 7 0.0 2 5 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 38 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 1 24 1 0.0 2 0 1 25 34 0.0 2 31 3 26 2 0.0 2 0 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 15 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.7% C: 0.0% G: 53.3% T: 20.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 14 0.0 2 9 5 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 31 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1.0 0 1 25 29 0.0 2 29 26 1 0.0 2 0 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep86_81_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep86_273_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep86_81_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep86_273_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (258 us/read; 0.23 M reads/minute). === Summary === Total read pairs processed: 135 Read 1 with adapter: 79 (58.5%) Read 2 with adapter: 78 (57.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 135 (100.0%) Total basepairs processed: 58,176 bp Read 1: 29,083 bp Read 2: 29,093 bp Total written (filtered): 54,255 bp (93.3%) Read 1: 27,111 bp Read 2: 27,144 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 16 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 87.5% C: 12.5% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 15 0.0 2 15 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 63 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 1 25 62 0.0 2 56 6 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 17 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.9% C: 0.0% G: 5.9% T: 88.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 17 0.0 2 16 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 61 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 60 0.0 2 59 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep90_82_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep90_274_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep90_82_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep90_274_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.04 s (300 us/read; 0.20 M reads/minute). === Summary === Total read pairs processed: 136 Read 1 with adapter: 113 (83.1%) Read 2 with adapter: 115 (84.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 136 (100.0%) Total basepairs processed: 56,815 bp Read 1: 28,404 bp Read 2: 28,411 bp Total written (filtered): 51,142 bp (90.0%) Read 1: 25,580 bp Read 2: 25,562 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 12 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.0% C: 16.7% G: 8.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 12 0.0 2 12 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 101 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 100 0.0 2 79 20 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 23 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.2% C: 0.0% G: 13.0% T: 34.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 23 0.0 2 11 12 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 92 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 2.1 0 1 24 4 0.0 2 0 4 25 87 0.0 2 85 2 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep92_83_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep92_275_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep92_83_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep92_275_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.17 s (192 us/read; 0.31 M reads/minute). === Summary === Total read pairs processed: 875 Read 1 with adapter: 764 (87.3%) Read 2 with adapter: 764 (87.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 875 (100.0%) Total basepairs processed: 415,575 bp Read 1: 207,805 bp Read 2: 207,770 bp Total written (filtered): 377,556 bp (90.9%) Read 1: 188,783 bp Read 2: 188,773 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 50 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 86.0% C: 14.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 25 50 0.0 2 50 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 714 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 13.7 0 1 4 1 3.4 0 1 21 1 0.0 2 0 0 1 23 5 0.0 2 0 2 3 24 23 0.0 2 5 15 3 25 681 0.0 2 653 28 26 2 0.0 2 0 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 56 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 3.6% C: 3.6% G: 75.0% T: 17.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 23 2 0.0 2 1 1 24 2 0.0 2 1 0 1 25 52 0.0 2 51 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 708 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 13.7 0 2 4 1 3.4 0 1 23 1 0.0 2 1 24 31 0.0 2 6 25 25 672 0.0 2 652 20 26 1 0.0 2 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep93_84_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep93_276_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep93_84_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep93_276_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 2.91 s (145 us/read; 0.41 M reads/minute). === Summary === Total read pairs processed: 20,085 Read 1 with adapter: 19,911 (99.1%) Read 2 with adapter: 19,925 (99.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 20,085 (100.0%) Total basepairs processed: 9,971,879 bp Read 1: 4,981,694 bp Read 2: 4,990,185 bp Total written (filtered): 8,977,744 bp (90.0%) Read 1: 4,485,052 bp Read 2: 4,492,692 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 78 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.7% C: 25.6% G: 7.7% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 313.8 0 4 24 2 0.0 2 0 2 25 72 0.0 2 70 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 19833 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 313.8 0 1 15 1 0.0 1 1 16 2 0.0 1 2 19 1 0.0 1 0 1 20 1 0.0 2 1 21 3 0.0 2 2 0 1 22 9 0.0 2 1 2 6 23 75 0.0 2 9 12 54 24 819 0.0 2 142 647 30 25 18895 0.0 2 18248 636 11 26 26 0.0 2 16 10 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 84 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 32.1% C: 0.0% G: 8.3% T: 59.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 2 25 82 0.0 2 56 22 4 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 19841 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 19 1 0.0 1 1 21 3 0.0 2 2 1 22 4 0.0 2 1 3 23 31 0.0 2 4 5 22 24 587 0.0 2 115 464 8 25 19190 0.0 2 18676 500 14 26 22 0.0 2 13 8 1 34 3 0.0 2 3 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep95_85_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep95_277_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep95_85_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep95_277_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.49 s (130 us/read; 0.46 M reads/minute). === Summary === Total read pairs processed: 11,417 Read 1 with adapter: 11,344 (99.4%) Read 2 with adapter: 11,349 (99.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 11,417 (100.0%) Total basepairs processed: 5,657,281 bp Read 1: 2,828,740 bp Read 2: 2,828,541 bp Total written (filtered): 5,090,892 bp (90.0%) Read 1: 2,545,695 bp Read 2: 2,545,197 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 100 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 84.0% C: 16.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 99 0.0 2 99 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 11244 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 178.4 0 2 16 1 0.0 1 0 1 20 1 0.0 2 1 21 4 0.0 2 3 0 1 22 3 0.0 2 0 2 1 23 33 0.0 2 3 11 19 24 418 0.0 2 67 334 17 25 10769 0.0 2 10398 363 8 26 13 0.0 2 9 4 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 103 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.4% C: 0.0% G: 26.2% T: 55.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 4 0.0 2 2 0 2 25 99 0.0 2 83 15 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 11246 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 4 1 44.6 0 1 21 2 0.0 2 2 22 6 0.0 2 2 3 1 23 16 0.0 2 2 1 13 24 309 0.0 2 59 244 6 25 10901 0.0 2 10590 306 5 26 11 0.0 2 9 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep96_86_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep96_278_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep96_86_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep96_278_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 3.07 s (136 us/read; 0.44 M reads/minute). === Summary === Total read pairs processed: 22,482 Read 1 with adapter: 21,339 (94.9%) Read 2 with adapter: 21,334 (94.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 22,482 (100.0%) Total basepairs processed: 11,092,106 bp Read 1: 5,546,212 bp Read 2: 5,545,894 bp Total written (filtered): 10,028,462 bp (90.4%) Read 1: 5,014,697 bp Read 2: 5,013,765 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 451 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 86.5% C: 6.4% G: 6.7% T: 0.4% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 28 351.3 0 28 4 2 87.8 0 2 19 1 0.0 1 1 24 2 0.0 2 1 1 25 416 0.0 2 413 3 26 2 0.0 2 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 20888 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 351.3 0 4 4 1 87.8 0 1 6 4 5.5 0 4 15 1 0.0 1 1 18 1 0.0 1 0 1 19 2 0.0 1 1 1 20 2 0.0 2 2 21 3 0.0 2 1 0 2 22 12 0.0 2 5 5 2 23 79 0.0 2 5 14 60 24 903 0.0 2 133 748 22 25 19839 0.0 2 19128 696 15 26 37 0.0 2 17 20 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 397 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.0% C: 1.3% G: 60.2% T: 34.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 1 87.8 0 1 24 2 0.0 2 1 1 25 393 0.0 2 377 11 5 28 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 20937 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 17 351.3 0 17 4 3 87.8 0 3 5 2 22.0 0 2 19 1 0.0 1 0 1 20 1 0.0 2 1 22 4 0.0 2 1 2 1 23 62 0.0 2 8 11 43 24 614 0.0 2 105 492 17 25 20207 0.0 2 19668 527 12 26 22 0.0 2 15 7 28 2 0.0 2 2 29 1 0.0 2 1 30 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep97_87_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep97_279_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep97_87_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep97_279_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (331 us/read; 0.18 M reads/minute). === Summary === Total read pairs processed: 78 Read 1 with adapter: 57 (73.1%) Read 2 with adapter: 57 (73.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 78 (100.0%) Total basepairs processed: 35,708 bp Read 1: 17,855 bp Read 2: 17,853 bp Total written (filtered): 32,862 bp (92.0%) Read 1: 16,430 bp Read 2: 16,432 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 7 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 85.7% C: 0.0% G: 14.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 7 0.0 2 7 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 50 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 25 50 0.0 2 46 4 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 5 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 60.0% T: 40.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 5 0.0 2 5 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 52 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 4 0.0 2 0 4 25 48 0.0 2 46 2 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm1_88_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm1_280_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm1_88_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm1_280_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 2.22 s (161 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 13,810 Read 1 with adapter: 13,723 (99.4%) Read 2 with adapter: 13,702 (99.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 13,810 (100.0%) Total basepairs processed: 6,765,814 bp Read 1: 3,380,036 bp Read 2: 3,385,778 bp Total written (filtered): 6,081,125 bp (89.9%) Read 1: 3,037,481 bp Read 2: 3,043,644 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 548 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 99.3% C: 0.4% G: 0.4% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 5 0.0 2 4 1 25 540 0.0 2 539 1 26 2 0.0 2 2 112 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 13175 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 21 3 0.0 2 2 1 22 1 0.0 2 1 23 39 0.0 2 3 10 26 24 530 0.0 2 81 430 19 25 12583 0.0 2 12053 521 9 26 19 0.0 2 9 10 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 509 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 2.2% C: 0.6% G: 91.9% T: 5.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 22 1 0.0 2 1 24 5 0.0 2 4 0 1 25 502 0.0 2 494 8 110 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 13193 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 215.8 0 1 15 1 0.0 1 0 1 20 2 0.0 2 1 1 21 3 0.0 2 1 1 1 22 5 0.0 2 3 1 1 23 29 0.0 2 5 4 20 24 385 0.0 2 68 310 7 25 12748 0.0 2 12366 367 15 26 19 0.0 2 13 6 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm103_89_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm103_281_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm103_89_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm103_281_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.09 s (219 us/read; 0.27 M reads/minute). === Summary === Total read pairs processed: 416 Read 1 with adapter: 187 (45.0%) Read 2 with adapter: 183 (44.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 416 (100.0%) Total basepairs processed: 189,538 bp Read 1: 94,735 bp Read 2: 94,803 bp Total written (filtered): 180,422 bp (95.2%) Read 1: 90,102 bp Read 2: 90,320 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 68 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.6% C: 1.5% G: 2.9% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 6.5 0 1 25 67 0.0 2 67 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 119 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 6.5 0 1 25 117 0.0 2 115 1 1 27 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 66 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 1.5% C: 1.5% G: 92.4% T: 4.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 1 24 1 0.0 2 1 25 64 0.0 2 63 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 117 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 6.5 0 4 24 2 0.0 2 0 2 25 110 0.0 2 107 3 26 1 0.0 2 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm104_90_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm104_282_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm104_90_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm104_282_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.04 s (242 us/read; 0.25 M reads/minute). === Summary === Total read pairs processed: 177 Read 1 with adapter: 167 (94.4%) Read 2 with adapter: 162 (91.5%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 177 (100.0%) Total basepairs processed: 81,419 bp Read 1: 40,717 bp Read 2: 40,702 bp Total written (filtered): 73,231 bp (89.9%) Read 1: 36,570 bp Read 2: 36,661 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 21 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 25 21 0.0 2 21 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 146 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 2.8 0 1 24 6 0.0 2 1 5 25 139 0.0 2 134 5 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 24 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 8.3% C: 4.2% G: 70.8% T: 16.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 24 0.0 2 23 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 138 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 0 1 24 7 0.0 2 0 6 1 25 130 0.0 2 123 7 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm105_91_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm105_283_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm105_91_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm105_283_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.98 s (178 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 5,497 Read 1 with adapter: 3,377 (61.4%) Read 2 with adapter: 3,349 (60.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 5,497 (100.0%) Total basepairs processed: 2,588,217 bp Read 1: 1,293,695 bp Read 2: 1,294,522 bp Total written (filtered): 2,421,772 bp (93.6%) Read 1: 1,209,671 bp Read 2: 1,212,101 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 799 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 98.4% C: 0.6% G: 0.8% T: 0.3% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 11 85.9 0 11 21 1 0.0 2 1 24 2 0.0 2 1 1 25 783 0.0 2 778 5 26 1 0.0 2 1 113 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 2578 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 5 85.9 0 5 4 1 21.5 0 1 21 1 0.0 2 1 23 11 0.0 2 2 4 5 24 100 0.0 2 10 86 4 25 2446 0.0 2 2334 92 20 26 13 0.0 2 7 4 2 27 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 750 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.8% C: 0.7% G: 94.0% T: 0.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 4 1 21.5 0 1 7 1 0.3 0 1 21 1 0.0 2 1 22 1 0.0 2 1 24 7 0.0 2 4 3 25 738 0.0 2 724 12 2 46 1 0.0 2 0 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 2599 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 48 85.9 0 48 4 4 21.5 0 4 5 2 5.4 0 2 22 2 0.0 2 1 0 1 23 4 0.0 2 0 0 4 24 80 0.0 2 14 63 3 25 2457 0.0 2 2380 74 3 26 2 0.0 2 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm110_92_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm110_284_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm110_92_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm110_284_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.49 s (152 us/read; 0.39 M reads/minute). === Summary === Total read pairs processed: 3,237 Read 1 with adapter: 2,632 (81.3%) Read 2 with adapter: 2,512 (77.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 3,237 (100.0%) Total basepairs processed: 1,280,552 bp Read 1: 640,267 bp Read 2: 640,285 bp Total written (filtered): 1,152,708 bp (90.0%) Read 1: 574,875 bp Read 2: 577,833 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 1168 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 93.5% C: 4.7% G: 1.8% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 13 50.6 0 13 4 1 12.6 0 1 23 1 0.0 2 1 24 9 0.0 2 7 2 25 1141 0.0 2 1134 6 1 26 2 0.0 2 2 112 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 1464 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 50.6 0 2 4 2 12.6 0 2 5 1 3.2 0 1 21 2 0.0 2 2 22 3 0.0 2 1 0 2 23 8 0.0 2 0 2 6 24 63 0.0 2 11 44 8 25 1361 0.0 2 1250 96 15 26 21 0.0 2 0 18 3 27 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 1072 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.5% C: 0.6% G: 79.6% T: 13.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 1 12.6 0 1 16 1 0.0 1 1 17 1 0.0 1 1 23 2 0.0 2 0 0 2 24 13 0.0 2 4 3 6 25 1053 0.0 2 983 58 12 112 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 1440 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 14 50.6 0 14 4 2 12.6 0 2 21 2 0.0 2 1 0 1 23 2 0.0 2 0 0 2 24 37 0.0 2 12 25 25 1376 0.0 2 1347 29 26 5 0.0 2 3 2 30 1 0.0 2 1 34 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm12_93_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm12_285_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm12_93_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm12_285_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.20 s (149 us/read; 0.40 M reads/minute). === Summary === Total read pairs processed: 1,348 Read 1 with adapter: 1,293 (95.9%) Read 2 with adapter: 1,277 (94.7%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,348 (100.0%) Total basepairs processed: 612,991 bp Read 1: 306,372 bp Read 2: 306,619 bp Total written (filtered): 548,914 bp (89.5%) Read 1: 274,099 bp Read 2: 274,815 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 259 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 96.1% C: 3.5% G: 0.4% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 5 0.0 2 4 1 25 254 0.0 2 253 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 1034 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 22 1 0.0 2 1 23 2 0.0 2 0 0 2 24 43 0.0 2 13 28 2 25 985 0.0 2 951 33 1 26 3 0.0 2 1 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 234 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.7% C: 0.9% G: 88.5% T: 6.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 4 1 5.3 0 1 6 2 0.3 0 2 24 2 0.0 2 2 25 227 0.0 2 223 3 1 26 1 0.0 2 0 1 39 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 1043 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 21.1 0 2 23 4 0.0 2 0 0 4 24 24 0.0 2 3 20 1 25 1012 0.0 2 978 34 26 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm13_94_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm13_286_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm13_94_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm13_286_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.34 s (177 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 1,897 Read 1 with adapter: 1,841 (97.0%) Read 2 with adapter: 1,834 (96.7%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,897 (100.0%) Total basepairs processed: 925,051 bp Read 1: 462,025 bp Read 2: 463,026 bp Total written (filtered): 833,469 bp (90.1%) Read 1: 416,110 bp Read 2: 417,359 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 68 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 89.7% C: 10.3% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 25 68 0.0 2 68 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 1773 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 29.6 0 1 22 1 0.0 2 1 23 8 0.0 2 1 3 4 24 70 0.0 2 6 61 3 25 1692 0.0 2 1638 53 1 26 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 61 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.6% C: 0.0% G: 65.6% T: 27.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 61 0.0 2 57 3 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 1773 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 5 29.6 0 5 22 2 0.0 2 0 0 2 23 3 0.0 2 0 1 2 24 62 0.0 2 14 47 1 25 1700 0.0 2 1628 70 2 26 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm19_95_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm19_287_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm19_95_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm19_287_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.29 s (162 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 7,981 Read 1 with adapter: 7,443 (93.3%) Read 2 with adapter: 7,346 (92.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 7,981 (100.0%) Total basepairs processed: 3,766,037 bp Read 1: 1,883,719 bp Read 2: 1,882,318 bp Total written (filtered): 3,397,170 bp (90.2%) Read 1: 1,698,169 bp Read 2: 1,699,001 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 1073 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 97.9% C: 1.1% G: 0.7% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 6 124.7 0 6 24 6 0.0 2 5 1 25 1059 0.0 2 1055 4 26 2 0.0 2 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 6370 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 124.7 0 2 20 2 0.0 2 1 1 21 1 0.0 2 1 22 3 0.0 2 2 1 23 29 0.0 2 2 7 20 24 273 0.0 2 35 223 15 25 6051 0.0 2 5818 226 7 26 9 0.0 2 4 5 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 1010 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 1.8% C: 1.2% G: 92.1% T: 5.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 2 124.7 0 2 16 2 0.0 1 2 19 1 0.0 1 0 1 23 1 0.0 2 1 24 15 0.0 2 10 4 1 25 987 0.0 2 965 19 3 26 2 0.0 2 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 6336 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 124.7 0 3 21 3 0.0 2 0 2 1 22 1 0.0 2 1 23 13 0.0 2 1 1 11 24 170 0.0 2 31 137 2 25 6132 0.0 2 5948 182 2 26 8 0.0 2 5 3 27 5 0.0 2 0 0 5 34 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm20_96_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm20_288_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm20_96_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm20_288_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 2.16 s (146 us/read; 0.41 M reads/minute). === Summary === Total read pairs processed: 14,827 Read 1 with adapter: 12,436 (83.9%) Read 2 with adapter: 12,168 (82.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 14,827 (100.0%) Total basepairs processed: 6,805,147 bp Read 1: 3,400,840 bp Read 2: 3,404,307 bp Total written (filtered): 6,191,291 bp (91.0%) Read 1: 3,090,850 bp Read 2: 3,100,441 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 2811 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.7% C: 2.6% G: 1.5% T: 0.3% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 16 231.7 0 16 5 1 14.5 0 1 18 1 0.0 1 0 1 22 2 0.0 2 2 23 3 0.0 2 2 0 1 24 19 0.0 2 11 7 1 25 2768 0.0 2 2759 9 26 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 9625 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 231.7 0 1 20 2 0.0 2 1 1 21 1 0.0 2 1 22 6 0.0 2 0 2 4 23 45 0.0 2 3 14 28 24 399 0.0 2 66 317 16 25 9132 0.0 2 8656 458 18 26 36 0.0 2 9 19 8 27 3 0.0 2 0 0 3 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 2730 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.5% C: 0.8% G: 90.8% T: 3.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 231.7 0 1 5 1 14.5 0 1 11 3 0.0 1 0 3 20 1 0.0 2 0 0 1 21 6 0.0 2 5 1 22 20 0.0 2 8 9 3 23 5 0.0 2 5 24 27 0.0 2 14 9 4 25 2657 0.0 2 2567 81 9 26 3 0.0 2 2 1 54 1 0.0 2 1 66 1 0.0 2 1 112 1 0.0 2 1 113 2 0.0 2 2 114 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 9438 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 10 231.7 0 10 4 1 57.9 0 1 22 2 0.0 2 0 0 2 23 25 0.0 2 2 4 19 24 273 0.0 2 48 219 6 25 9109 0.0 2 8794 310 5 26 16 0.0 2 10 6 27 1 0.0 2 0 0 1 28 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm21_97_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm21_289_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm21_97_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm21_289_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.25 s (127 us/read; 0.47 M reads/minute). === Summary === Total read pairs processed: 1,957 Read 1 with adapter: 1,805 (92.2%) Read 2 with adapter: 1,747 (89.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,957 (100.0%) Total basepairs processed: 661,793 bp Read 1: 331,069 bp Read 2: 330,724 bp Total written (filtered): 573,016 bp (86.6%) Read 1: 285,832 bp Read 2: 287,184 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 945 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 83.1% C: 15.1% G: 1.5% T: 0.3% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 0 1 24 14 0.0 2 10 2 2 25 928 0.0 2 923 5 113 2 0.0 2 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 860 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 2 0.0 2 0 0 2 24 48 0.0 2 7 32 9 25 806 0.0 2 727 73 6 26 4 0.0 2 0 4 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 802 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.6% C: 1.2% G: 68.3% T: 25.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 17 1 0.0 1 1 23 1 0.0 2 1 24 16 0.0 2 12 1 3 25 784 0.0 2 747 31 6 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 945 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 30.6 0 3 4 1 7.6 0 1 23 2 0.0 2 0 0 2 24 24 0.0 2 5 19 25 911 0.0 2 890 21 26 2 0.0 2 1 1 27 2 0.0 2 0 0 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm22_98_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm22_290_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm22_98_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm22_290_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.39 s (137 us/read; 0.44 M reads/minute). === Summary === Total read pairs processed: 2,836 Read 1 with adapter: 2,705 (95.4%) Read 2 with adapter: 2,674 (94.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 2,836 (100.0%) Total basepairs processed: 1,283,926 bp Read 1: 642,774 bp Read 2: 641,152 bp Total written (filtered): 1,149,707 bp (89.5%) Read 1: 575,278 bp Read 2: 574,429 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 545 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 87.0% C: 1.7% G: 0.7% T: 10.6% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 44.3 0 1 24 4 0.0 2 4 25 540 0.0 2 538 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 2160 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 22 1 0.0 2 0 0 1 23 15 0.0 2 3 2 10 24 77 0.0 2 17 57 3 25 2060 0.0 2 1963 95 2 26 7 0.0 2 0 6 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 538 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 13.0% C: 3.7% G: 76.0% T: 7.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 1 11.1 0 1 21 1 0.0 2 0 1 23 1 0.0 2 0 0 1 24 5 0.0 2 4 0 1 25 529 0.0 2 508 17 4 26 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 2136 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 44.3 0 1 23 4 0.0 2 1 0 3 24 76 0.0 2 9 64 3 25 2048 0.0 2 1984 64 26 4 0.0 2 3 1 27 3 0.0 2 0 0 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm23_99_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm23_291_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm23_99_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm23_291_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.21 s (172 us/read; 0.35 M reads/minute). === Summary === Total read pairs processed: 1,225 Read 1 with adapter: 965 (78.8%) Read 2 with adapter: 913 (74.5%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,225 (100.0%) Total basepairs processed: 503,215 bp Read 1: 251,572 bp Read 2: 251,643 bp Total written (filtered): 456,510 bp (90.7%) Read 1: 227,531 bp Read 2: 228,979 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 296 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 81.8% C: 17.2% G: 1.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 2 19.1 0 2 25 294 0.0 2 291 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 669 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 19.1 0 1 23 3 0.0 2 0 1 2 24 22 0.0 2 2 19 1 25 635 0.0 2 580 48 7 26 6 0.0 2 2 4 27 2 0.0 2 0 0 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 284 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.2% C: 1.1% G: 62.7% T: 27.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 19.1 0 1 4 1 4.8 0 1 5 1 1.2 0 1 16 2 0.0 1 2 17 1 0.0 1 1 21 2 0.0 2 2 22 4 0.0 2 3 1 23 1 0.0 2 0 1 24 5 0.0 2 3 2 25 264 0.0 2 244 16 4 26 1 0.0 2 1 66 1 0.0 2 0 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 629 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 19.1 0 2 4 1 4.8 0 1 23 1 0.0 2 1 24 21 0.0 2 0 19 2 25 603 0.0 2 590 13 26 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm30_100_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm30_292_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm30_100_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm30_292_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (254 us/read; 0.24 M reads/minute). === Summary === Total read pairs processed: 109 Read 1 with adapter: 85 (78.0%) Read 2 with adapter: 85 (78.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 109 (100.0%) Total basepairs processed: 41,450 bp Read 1: 20,704 bp Read 2: 20,746 bp Total written (filtered): 37,206 bp (89.8%) Read 1: 18,582 bp Read 2: 18,624 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 40 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.0% C: 5.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 39 0.0 2 39 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 45 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 3 0.0 2 1 2 25 41 0.0 2 39 2 26 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 37 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.4% C: 2.7% G: 81.1% T: 10.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 36 0.0 2 36 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 48 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 1 25 46 0.0 2 45 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm31_101_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm31_293_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm31_101_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm31_293_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.08 s (148 us/read; 0.40 M reads/minute). === Summary === Total read pairs processed: 559 Read 1 with adapter: 535 (95.7%) Read 2 with adapter: 530 (94.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 559 (100.0%) Total basepairs processed: 239,378 bp Read 1: 119,586 bp Read 2: 119,792 bp Total written (filtered): 212,709 bp (88.9%) Read 1: 106,152 bp Read 2: 106,557 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 140 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 93.6% C: 5.7% G: 0.7% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 3 0.0 2 1 2 25 136 0.0 2 135 1 112 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 395 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 3 0.0 2 0 0 3 24 20 0.0 2 1 18 1 25 371 0.0 2 351 17 3 26 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 140 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.7% C: 0.0% G: 85.7% T: 8.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 25 140 0.0 2 133 5 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 390 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 0 1 24 15 0.0 2 4 10 1 25 372 0.0 2 359 13 26 2 0.0 2 0 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm33_102_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm33_294_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm33_102_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm33_294_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (366 us/read; 0.16 M reads/minute). === Summary === Total read pairs processed: 87 Read 1 with adapter: 75 (86.2%) Read 2 with adapter: 73 (83.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 87 (100.0%) Total basepairs processed: 33,774 bp Read 1: 16,880 bp Read 2: 16,894 bp Total written (filtered): 30,103 bp (89.1%) Read 1: 15,031 bp Read 2: 15,072 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 31 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 90.3% C: 9.7% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 25 31 0.0 2 31 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 44 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1.4 0 1 24 4 0.0 2 2 1 1 25 39 0.0 2 36 3 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 30 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 3.3% C: 0.0% G: 86.7% T: 10.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 29 0.0 2 27 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 43 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 0 2 25 41 0.0 2 41 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm34_103_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm34_295_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm34_103_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm34_295_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (232 us/read; 0.26 M reads/minute). === Summary === Total read pairs processed: 115 Read 1 with adapter: 93 (80.9%) Read 2 with adapter: 89 (77.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 115 (100.0%) Total basepairs processed: 39,498 bp Read 1: 19,731 bp Read 2: 19,767 bp Total written (filtered): 34,950 bp (88.5%) Read 1: 17,409 bp Read 2: 17,541 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 57 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 86.0% C: 14.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 25 57 0.0 2 57 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 36 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 0 1 24 1 0.0 2 0 1 25 34 0.0 2 30 4 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 47 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 2.1% C: 0.0% G: 72.3% T: 25.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 47 0.0 2 46 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 42 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 25 41 0.0 2 40 1 26 1 0.0 2 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm35_104_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm35_296_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm35_104_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm35_296_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (511 us/read; 0.12 M reads/minute). === Summary === Total read pairs processed: 54 Read 1 with adapter: 43 (79.6%) Read 2 with adapter: 42 (77.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 54 (100.0%) Total basepairs processed: 20,105 bp Read 1: 10,054 bp Read 2: 10,051 bp Total written (filtered): 18,003 bp (89.5%) Read 1: 8,980 bp Read 2: 9,023 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 24 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.8% C: 4.2% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 25 24 0.0 2 24 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 19 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 18 0.0 2 17 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 26 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.5% C: 0.0% G: 80.8% T: 7.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 25 0.0 2 25 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 16 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 4 1 0.2 0 1 25 15 0.0 2 15 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm36_105_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm36_297_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm36_105_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm36_297_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.05 s (235 us/read; 0.25 M reads/minute). === Summary === Total read pairs processed: 203 Read 1 with adapter: 192 (94.6%) Read 2 with adapter: 184 (90.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 203 (100.0%) Total basepairs processed: 77,077 bp Read 1: 38,532 bp Read 2: 38,545 bp Total written (filtered): 67,682 bp (87.8%) Read 1: 33,737 bp Read 2: 33,945 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 71 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 83.1% C: 11.3% G: 5.6% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 70 0.0 2 69 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 121 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 1 24 5 0.0 2 0 5 25 113 0.0 2 99 14 26 1 0.0 2 1 27 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 59 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.9% C: 0.0% G: 64.4% T: 23.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 59 0.0 2 52 6 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 125 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 1 25 121 0.0 2 113 7 1 26 2 0.0 2 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm37_106_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm37_298_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm37_106_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm37_298_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.45 s (144 us/read; 0.42 M reads/minute). === Summary === Total read pairs processed: 3,148 Read 1 with adapter: 2,660 (84.5%) Read 2 with adapter: 2,637 (83.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 3,148 (100.0%) Total basepairs processed: 1,372,127 bp Read 1: 685,934 bp Read 2: 686,193 bp Total written (filtered): 1,240,090 bp (90.4%) Read 1: 619,547 bp Read 2: 620,543 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 597 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 88.8% C: 10.1% G: 1.2% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 1 22 2 0.0 2 2 23 1 0.0 2 1 24 6 0.0 2 3 3 25 587 0.0 2 579 7 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 2063 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 4 1 12.3 0 1 21 1 0.0 2 0 1 23 4 0.0 2 0 0 4 24 68 0.0 2 1 25 42 25 1984 0.0 2 552 1378 54 26 4 0.0 2 1 3 27 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 567 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 7.1% C: 0.7% G: 78.7% T: 13.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 1 12.3 0 1 8 1 0.0 0 1 16 1 0.0 1 1 21 1 0.0 2 1 22 1 0.0 2 1 24 9 0.0 2 6 1 2 25 553 0.0 2 521 25 7 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 2070 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 6 49.2 0 6 22 3 0.0 2 1 1 1 23 7 0.0 2 2 0 5 24 61 0.0 2 15 44 2 25 1989 0.0 2 1920 68 1 26 4 0.0 2 2 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm4_107_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm4_299_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm4_107_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm4_299_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 2.90 s (164 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 17,703 Read 1 with adapter: 17,614 (99.5%) Read 2 with adapter: 17,632 (99.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 17,703 (100.0%) Total basepairs processed: 8,625,637 bp Read 1: 4,309,145 bp Read 2: 4,316,492 bp Total written (filtered): 7,745,978 bp (89.8%) Read 1: 3,869,641 bp Read 2: 3,876,337 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 451 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 86.3% C: 13.3% G: 0.4% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 276.6 0 1 24 3 0.0 2 2 1 25 447 0.0 2 442 5 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 17163 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 19 3 0.0 1 2 1 20 3 0.0 2 0 2 1 21 1 0.0 2 1 22 5 0.0 2 2 1 2 23 60 0.0 2 7 10 43 24 680 0.0 2 121 544 15 25 16380 0.0 2 15788 580 12 26 31 0.0 2 19 12 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 409 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.6% C: 1.0% G: 1.5% T: 92.9% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 3 0.0 2 3 25 406 0.0 2 384 17 5 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 17223 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 4 1 69.2 0 1 16 1 0.0 1 0 1 18 1 0.0 1 1 20 1 0.0 2 1 21 4 0.0 2 2 1 1 22 5 0.0 2 2 1 2 23 40 0.0 2 7 6 27 24 516 0.0 2 88 410 18 25 16632 0.0 2 16128 493 11 26 21 0.0 2 16 5 31 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm41_108_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm41_300_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm41_108_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm41_300_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.25 s (174 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 1,416 Read 1 with adapter: 290 (20.5%) Read 2 with adapter: 285 (20.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,416 (100.0%) Total basepairs processed: 622,468 bp Read 1: 311,014 bp Read 2: 311,454 bp Total written (filtered): 608,141 bp (97.7%) Read 1: 303,789 bp Read 2: 304,352 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 180 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 68.9% C: 27.8% G: 3.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 22.1 0 1 24 1 0.0 2 0 1 25 178 0.0 2 175 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 110 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 0 1 1 25 108 0.0 2 91 14 3 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 156 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.0% C: 1.9% G: 43.6% T: 45.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 0 1 25 153 0.0 2 139 11 3 26 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 129 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 5 1 1.4 0 1 24 2 0.0 2 1 1 25 126 0.0 2 123 3 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm44_109_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm44_301_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm44_109_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm44_301_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.07 s (183 us/read; 0.33 M reads/minute). === Summary === Total read pairs processed: 409 Read 1 with adapter: 329 (80.4%) Read 2 with adapter: 318 (77.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 409 (100.0%) Total basepairs processed: 161,455 bp Read 1: 80,794 bp Read 2: 80,661 bp Total written (filtered): 145,429 bp (90.1%) Read 1: 72,623 bp Read 2: 72,806 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 87 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 80.5% C: 16.1% G: 3.4% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 2 6.4 0 2 25 85 0.0 2 83 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 242 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 12 0.0 2 2 7 3 25 229 0.0 2 210 18 1 27 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 79 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 8.9% C: 0.0% G: 54.4% T: 36.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 5 0.0 2 2 0 3 25 74 0.0 2 68 5 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 239 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 6.4 0 4 24 3 0.0 2 1 2 25 231 0.0 2 222 9 26 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm48_110_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm48_302_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm48_110_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm48_302_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.16 s (163 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 983 Read 1 with adapter: 926 (94.2%) Read 2 with adapter: 907 (92.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 983 (100.0%) Total basepairs processed: 394,610 bp Read 1: 197,202 bp Read 2: 197,408 bp Total written (filtered): 349,245 bp (88.5%) Read 1: 174,445 bp Read 2: 174,800 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 342 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 87.1% C: 12.3% G: 0.6% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 20 69 0.0 2 69 21 1 0.0 2 1 22 1 0.0 2 1 23 2 0.0 2 2 24 7 0.0 2 2 4 1 25 262 0.0 2 261 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 584 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 22 1 0.0 2 1 23 2 0.0 2 0 1 1 24 24 0.0 2 6 13 5 25 556 0.0 2 508 44 4 26 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 242 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.0% C: 3.7% G: 67.4% T: 24.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 3 0.0 1 2 1 20 2 0.0 2 2 21 3 0.0 2 3 24 2 0.0 2 1 0 1 25 232 0.0 2 221 8 3 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 665 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 22 1 0.0 2 0 0 1 24 22 0.0 2 6 15 1 25 642 0.0 2 633 9 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm51_111_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm51_303_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm51_111_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm51_303_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.03 s (314 us/read; 0.19 M reads/minute). === Summary === Total read pairs processed: 84 Read 1 with adapter: 78 (92.9%) Read 2 with adapter: 77 (91.7%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 84 (100.0%) Total basepairs processed: 35,424 bp Read 1: 17,706 bp Read 2: 17,718 bp Total written (filtered): 31,553 bp (89.1%) Read 1: 15,757 bp Read 2: 15,796 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 19 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 89.5% C: 5.3% G: 5.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 19 0.0 2 19 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 59 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 1 25 56 0.0 2 51 4 1 26 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 18 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 83.3% T: 16.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 17 0.0 2 16 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 59 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 0 2 25 57 0.0 2 55 2 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm59_112_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm59_304_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm59_112_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm59_304_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.16 s (198 us/read; 0.30 M reads/minute). === Summary === Total read pairs processed: 783 Read 1 with adapter: 545 (69.6%) Read 2 with adapter: 536 (68.5%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 783 (100.0%) Total basepairs processed: 349,983 bp Read 1: 175,000 bp Read 2: 174,983 bp Total written (filtered): 323,015 bp (92.3%) Read 1: 161,360 bp Read 2: 161,655 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 135 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 85.9% C: 12.6% G: 0.7% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 2 12.2 0 2 24 2 0.0 2 2 25 130 0.0 2 129 1 112 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 410 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 22 1 0.0 2 0 1 23 2 0.0 2 2 24 20 0.0 2 1 17 2 25 386 0.0 2 362 22 2 26 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 114 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.4% C: 6.1% G: 57.9% T: 31.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 22 1 0.0 2 1 23 1 0.0 2 1 25 111 0.0 2 106 3 2 34 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 422 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 12.2 0 2 5 1 0.8 0 1 23 1 0.0 2 0 0 1 24 10 0.0 2 3 7 25 408 0.0 2 398 10 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm6_113_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm6_305_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm6_113_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm6_305_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 10.66 s (157 us/read; 0.38 M reads/minute). === Summary === Total read pairs processed: 68,078 Read 1 with adapter: 67,768 (99.5%) Read 2 with adapter: 67,751 (99.5%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 68,078 (100.0%) Total basepairs processed: 33,383,438 bp Read 1: 16,686,114 bp Read 2: 16,697,324 bp Total written (filtered): 30,001,502 bp (89.9%) Read 1: 14,995,534 bp Read 2: 15,005,968 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 1961 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 84.8% C: 13.2% G: 2.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 4 1063.7 0 4 4 1 265.9 0 1 18 1 0.0 1 1 23 3 0.0 2 2 0 1 24 19 0.0 2 8 10 1 25 1929 0.0 2 1912 17 26 4 0.0 2 4 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 65807 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 13 1 0.0 1 1 14 1 0.0 1 0 1 15 3 0.0 1 1 2 16 1 0.0 1 0 1 18 2 0.0 1 1 1 19 6 0.0 1 5 0 1 20 3 0.0 2 1 2 21 17 0.0 2 15 1 1 22 24 0.0 2 9 3 12 23 286 0.0 2 22 65 199 24 2737 0.0 2 416 2243 78 25 62636 0.0 2 59794 2766 76 26 87 0.0 2 43 44 27 3 0.0 2 1 1 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 1688 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.8% C: 0.4% G: 69.7% T: 23.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 8 1 1.0 0 1 16 1 0.0 1 1 22 1 0.0 2 1 23 1 0.0 2 0 1 24 21 0.0 2 13 3 5 25 1661 0.0 2 1598 55 8 26 2 0.0 2 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 66063 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 1063.7 0 2 4 1 265.9 0 1 18 1 0.0 1 1 19 2 0.0 1 2 21 5 0.0 2 3 2 22 15 0.0 2 8 2 5 23 140 0.0 2 18 22 100 24 2004 0.0 2 293 1659 52 25 63831 0.0 2 61821 1957 53 26 61 0.0 2 38 22 1 28 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm61_114_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm61_306_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm61_114_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm61_306_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.12 s (177 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 665 Read 1 with adapter: 591 (88.9%) Read 2 with adapter: 588 (88.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 665 (100.0%) Total basepairs processed: 288,666 bp Read 1: 144,589 bp Read 2: 144,077 bp Total written (filtered): 259,133 bp (89.8%) Read 1: 129,775 bp Read 2: 129,358 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 147 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 93.9% C: 4.8% G: 1.4% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 10.4 0 1 24 3 0.0 2 2 1 25 142 0.0 2 140 2 113 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 444 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 0 0 1 23 1 0.0 2 0 0 1 24 20 0.0 2 4 14 2 25 420 0.0 2 397 20 3 26 2 0.0 2 0 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 138 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.8% C: 0.7% G: 84.1% T: 9.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 0 1 24 4 0.0 2 3 0 1 25 131 0.0 2 119 8 4 26 1 0.0 2 1 113 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 450 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 10.4 0 2 23 1 0.0 2 0 0 1 24 16 0.0 2 5 11 25 431 0.0 2 419 12 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm66_115_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm66_307_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm66_115_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm66_307_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 2.22 s (164 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 13,547 Read 1 with adapter: 13,450 (99.3%) Read 2 with adapter: 13,452 (99.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 13,547 (100.0%) Total basepairs processed: 6,676,870 bp Read 1: 3,337,774 bp Read 2: 3,339,096 bp Total written (filtered): 6,005,490 bp (89.9%) Read 1: 3,002,153 bp Read 2: 3,003,337 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 227 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 81.1% C: 16.7% G: 2.2% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 6 0.0 2 1 5 25 221 0.0 2 218 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 13223 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 14 1 0.0 1 1 20 2 0.0 2 1 1 21 1 0.0 2 0 1 22 4 0.0 2 2 0 2 23 42 0.0 2 7 9 26 24 531 0.0 2 65 455 11 25 12615 0.0 2 12131 475 9 26 25 0.0 2 17 8 27 2 0.0 2 0 0 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 195 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.7% C: 0.5% G: 61.5% T: 31.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 3 0.0 2 1 2 25 192 0.0 2 178 12 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 13257 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 4 2 52.9 0 2 21 3 0.0 2 2 0 1 22 5 0.0 2 3 1 1 23 33 0.0 2 3 8 22 24 419 0.0 2 68 342 9 25 12781 0.0 2 12334 438 9 26 13 0.0 2 8 5 28 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm74_116_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm74_308_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm74_116_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm74_308_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.07 s (209 us/read; 0.29 M reads/minute). === Summary === Total read pairs processed: 342 Read 1 with adapter: 296 (86.5%) Read 2 with adapter: 290 (84.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 342 (100.0%) Total basepairs processed: 138,202 bp Read 1: 69,104 bp Read 2: 69,098 bp Total written (filtered): 123,594 bp (89.4%) Read 1: 61,718 bp Read 2: 61,876 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 86 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 90.7% C: 4.7% G: 1.2% T: 3.5% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 1 25 85 0.0 2 82 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 210 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 0 1 24 10 0.0 2 1 7 2 25 197 0.0 2 174 21 2 26 2 0.0 2 1 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 92 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.1% C: 0.0% G: 78.3% T: 7.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 0 1 25 90 0.0 2 77 11 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 198 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 5.3 0 1 22 1 0.0 2 1 24 3 0.0 2 0 3 25 192 0.0 2 185 7 27 1 0.0 2 0 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm75_117_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm75_309_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm75_117_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm75_309_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.05 s (230 us/read; 0.26 M reads/minute). === Summary === Total read pairs processed: 214 Read 1 with adapter: 197 (92.1%) Read 2 with adapter: 192 (89.7%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 214 (100.0%) Total basepairs processed: 77,901 bp Read 1: 38,945 bp Read 2: 38,956 bp Total written (filtered): 68,197 bp (87.5%) Read 1: 34,033 bp Read 2: 34,164 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 78 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.9% C: 23.1% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 1 25 76 0.0 2 74 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 119 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 0 1 24 9 0.0 2 0 9 25 109 0.0 2 103 4 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 67 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.5% C: 0.0% G: 55.2% T: 40.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 66 0.0 2 63 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 125 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 7 0.0 2 0 7 25 118 0.0 2 114 4 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm76_118_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm76_310_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm76_118_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm76_310_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.06 s (340 us/read; 0.18 M reads/minute). === Summary === Total read pairs processed: 185 Read 1 with adapter: 105 (56.8%) Read 2 with adapter: 100 (54.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 185 (100.0%) Total basepairs processed: 84,915 bp Read 1: 42,430 bp Read 2: 42,485 bp Total written (filtered): 79,820 bp (94.0%) Read 1: 39,834 bp Read 2: 39,986 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 25 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 88.0% C: 8.0% G: 4.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 25 25 0.0 2 24 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 80 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 2.9 0 1 24 7 0.0 2 0 6 1 25 72 0.0 2 69 3 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 26 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 7.7% C: 3.8% G: 73.1% T: 15.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 0 1 25 25 0.0 2 25 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 74 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 25 74 0.0 2 72 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm77_119_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm77_311_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm77_119_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm77_311_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.05 s (198 us/read; 0.30 M reads/minute). === Summary === Total read pairs processed: 260 Read 1 with adapter: 134 (51.5%) Read 2 with adapter: 132 (50.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 260 (100.0%) Total basepairs processed: 112,882 bp Read 1: 56,419 bp Read 2: 56,463 bp Total written (filtered): 106,235 bp (94.1%) Read 1: 53,071 bp Read 2: 53,164 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 50 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 90.0% C: 8.0% G: 2.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 25 50 0.0 2 49 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 84 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 3 0.0 2 0 3 25 80 0.0 2 75 5 26 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 44 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 77.3% T: 22.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 44 0.0 2 44 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 88 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 0 0 1 24 2 0.0 2 1 1 25 82 0.0 2 80 1 1 26 3 0.0 2 0 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm79_120_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm79_312_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm79_120_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm79_312_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.02 s (438 us/read; 0.14 M reads/minute). === Summary === Total read pairs processed: 47 Read 1 with adapter: 21 (44.7%) Read 2 with adapter: 20 (42.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 47 (100.0%) Total basepairs processed: 21,010 bp Read 1: 10,499 bp Read 2: 10,511 bp Total written (filtered): 19,985 bp (95.1%) Read 1: 9,973 bp Read 2: 10,012 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 8 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 8 0.0 2 8 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 13 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 25 12 0.0 2 11 1 26 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 8 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 12.5% C: 0.0% G: 75.0% T: 12.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 7 0.0 2 7 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 12 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 25 12 0.0 2 10 2 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm80_121_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm80_313_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm80_121_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm80_313_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.04 s (245 us/read; 0.25 M reads/minute). === Summary === Total read pairs processed: 170 Read 1 with adapter: 90 (52.9%) Read 2 with adapter: 80 (47.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 170 (100.0%) Total basepairs processed: 75,811 bp Read 1: 37,866 bp Read 2: 37,945 bp Total written (filtered): 71,613 bp (94.5%) Read 1: 35,621 bp Read 2: 35,992 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 31 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.4% C: 16.1% G: 6.5% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 0 2 25 29 0.0 2 26 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 59 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 3 0.0 2 1 2 25 56 0.0 2 47 7 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 34 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.6% C: 0.0% G: 67.6% T: 11.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 34 0.0 2 27 5 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 46 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 2.7 0 2 24 3 0.0 2 1 2 25 41 0.0 2 39 2 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm84_122_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm84_314_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm84_122_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm84_314_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.02 s (409 us/read; 0.15 M reads/minute). === Summary === Total read pairs processed: 42 Read 1 with adapter: 38 (90.5%) Read 2 with adapter: 38 (90.5%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 42 (100.0%) Total basepairs processed: 17,473 bp Read 1: 8,735 bp Read 2: 8,738 bp Total written (filtered): 15,572 bp (89.1%) Read 1: 7,785 bp Read 2: 7,787 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 10 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 10 0.0 2 10 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 28 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 1 25 25 0.0 2 24 1 27 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 11 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 9.1% G: 81.8% T: 9.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 9 0.0 2 9 26 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 27 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 25 26 0.0 2 24 2 26 1 0.0 2 0 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm85_123_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm85_315_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm85_123_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm85_315_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.06 s (224 us/read; 0.27 M reads/minute). === Summary === Total read pairs processed: 275 Read 1 with adapter: 262 (95.3%) Read 2 with adapter: 259 (94.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 275 (100.0%) Total basepairs processed: 120,763 bp Read 1: 60,288 bp Read 2: 60,475 bp Total written (filtered): 107,685 bp (89.2%) Read 1: 53,743 bp Read 2: 53,942 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 50 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 94.0% C: 6.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 25 50 0.0 2 50 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 212 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 7 0.0 2 2 5 25 203 0.0 2 193 9 1 26 2 0.0 2 0 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 58 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.9% C: 3.4% G: 70.7% T: 19.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 56 0.0 2 52 3 1 113 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 201 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 4.3 0 1 23 1 0.0 2 0 0 1 24 6 0.0 2 3 3 25 192 0.0 2 186 6 26 1 0.0 2 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm86_124_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm86_316_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm86_124_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm86_316_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.09 s (175 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 534 Read 1 with adapter: 482 (90.3%) Read 2 with adapter: 470 (88.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 534 (100.0%) Total basepairs processed: 215,983 bp Read 1: 107,926 bp Read 2: 108,057 bp Total written (filtered): 192,232 bp (89.0%) Read 1: 95,935 bp Read 2: 96,297 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 158 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 90.5% C: 7.6% G: 1.3% T: 0.6% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 1 24 1 0.0 2 1 25 156 0.0 2 155 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 324 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 8.3 0 1 17 1 0.0 1 1 23 1 0.0 2 0 1 24 26 0.0 2 5 12 9 25 291 0.0 2 272 18 1 26 4 0.0 2 0 3 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 156 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 7.7% C: 0.6% G: 84.0% T: 7.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 1 24 2 0.0 2 1 0 1 25 151 0.0 2 140 10 1 26 1 0.0 2 1 54 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 314 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 15 0.0 2 2 13 25 298 0.0 2 285 12 1 26 1 0.0 2 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm90_125_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm90_317_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm90_125_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm90_317_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.14 s (168 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 841 Read 1 with adapter: 274 (32.6%) Read 2 with adapter: 264 (31.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 841 (100.0%) Total basepairs processed: 397,265 bp Read 1: 198,520 bp Read 2: 198,745 bp Total written (filtered): 383,901 bp (96.6%) Read 1: 191,707 bp Read 2: 192,194 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 66 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 81.8% C: 18.2% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 13.1 0 1 24 2 0.0 2 1 1 25 63 0.0 2 63 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 208 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 23 2 0.0 2 1 0 1 24 9 0.0 2 1 7 1 25 197 0.0 2 180 15 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 59 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.8% C: 5.1% G: 27.1% T: 61.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 1 3.3 0 1 24 1 0.0 2 0 1 25 57 0.0 2 54 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 205 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 13.1 0 1 23 1 0.0 2 0 0 1 24 4 0.0 2 0 4 25 198 0.0 2 195 3 26 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm92_126_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm92_318_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm92_126_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm92_318_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.06 s (299 us/read; 0.20 M reads/minute). === Summary === Total read pairs processed: 197 Read 1 with adapter: 187 (94.9%) Read 2 with adapter: 183 (92.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 197 (100.0%) Total basepairs processed: 88,470 bp Read 1: 44,305 bp Read 2: 44,165 bp Total written (filtered): 79,263 bp (89.6%) Read 1: 39,664 bp Read 2: 39,599 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 27 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 96.3% C: 0.0% G: 3.7% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 2 25 25 0.0 2 25 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 160 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 3.1 0 1 23 1 0.0 2 0 0 1 24 9 0.0 2 0 9 25 148 0.0 2 140 8 26 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 31 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.1% C: 3.2% G: 80.6% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 22 1 0.0 2 0 1 24 1 0.0 2 0 1 25 29 0.0 2 27 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 152 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 5 0.0 2 1 4 25 147 0.0 2 139 8 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm95_127_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm95_319_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm95_127_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm95_319_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.11 s (178 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 595 Read 1 with adapter: 262 (44.0%) Read 2 with adapter: 257 (43.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 595 (100.0%) Total basepairs processed: 271,841 bp Read 1: 135,873 bp Read 2: 135,968 bp Total written (filtered): 259,038 bp (95.3%) Read 1: 129,398 bp Read 2: 129,640 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 59 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 74.6% C: 25.4% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 58 0.0 2 57 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 203 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 9.3 0 3 23 2 0.0 2 0 0 2 24 5 0.0 2 0 5 25 192 0.0 2 174 15 3 26 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 48 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 12.5% C: 0.0% G: 52.1% T: 35.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 48 0.0 2 40 7 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 209 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 9.3 0 4 24 9 0.0 2 2 7 25 196 0.0 2 190 6 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm96_128_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm96_320_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm96_128_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm96_320_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.02 s (324 us/read; 0.19 M reads/minute). === Summary === Total read pairs processed: 71 Read 1 with adapter: 22 (31.0%) Read 2 with adapter: 22 (31.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 71 (100.0%) Total basepairs processed: 31,826 bp Read 1: 15,903 bp Read 2: 15,923 bp Total written (filtered): 30,750 bp (96.6%) Read 1: 15,354 bp Read 2: 15,396 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 7 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 85.7% C: 0.0% G: 14.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 7 0.0 2 6 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 15 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 14 0.0 2 14 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 5 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.0% C: 0.0% G: 80.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 5 0.0 2 5 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 17 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1.1 0 1 24 2 0.0 2 0 2 25 13 0.0 2 13 26 1 0.0 2 0 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O10_129_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O10_321_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O10_129_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O10_321_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 37.09 s (153 us/read; 0.39 M reads/minute). === Summary === Total read pairs processed: 243,135 Read 1 with adapter: 224,634 (92.4%) Read 2 with adapter: 224,605 (92.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 243,135 (100.0%) Total basepairs processed: 120,036,986 bp Read 1: 59,967,145 bp Read 2: 60,069,841 bp Total written (filtered): 108,850,890 bp (90.7%) Read 1: 54,376,628 bp Read 2: 54,474,262 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 5721 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 87.9% C: 2.3% G: 9.5% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 489 3799.0 0 489 4 3 949.7 0 3 18 1 0.0 1 1 19 309 0.0 1 301 8 20 457 0.0 2 442 14 1 21 24 0.0 2 23 1 22 5 0.0 2 5 23 2 0.0 2 1 1 24 43 0.0 2 28 15 25 4381 0.0 2 4366 15 26 1 0.0 2 1 88 1 0.0 2 1 89 4 0.0 2 4 227 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 218913 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 102 3799.0 0 102 4 7 949.7 0 7 5 3 237.4 0 3 6 1 59.4 0 1 11 1 0.1 1 1 14 1 0.0 1 0 1 15 2 0.0 1 0 2 16 2 0.0 1 1 1 17 1 0.0 1 1 18 2 0.0 1 1 1 19 25 0.0 1 17 7 1 20 19 0.0 2 11 4 4 21 27 0.0 2 11 8 8 22 66 0.0 2 33 11 22 23 594 0.0 2 81 142 371 24 6699 0.0 2 1304 5262 133 25 211112 0.0 2 205107 5901 104 26 243 0.0 2 152 91 27 2 0.0 2 1 0 1 28 1 0.0 2 1 29 2 0.0 2 2 30 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 4882 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.4% C: 0.5% G: 48.6% T: 44.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 3799.0 0 2 19 408 0.0 1 405 3 20 18 0.0 2 12 6 21 11 0.0 2 7 3 1 22 5 0.0 2 3 2 23 5 0.0 2 2 3 24 66 0.0 2 39 14 13 25 4354 0.0 2 4051 265 38 26 1 0.0 2 1 46 1 0.0 2 1 51 2 0.0 2 2 88 3 0.0 2 3 89 6 0.0 2 6 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 219723 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 458 3799.0 0 458 4 46 949.7 0 46 5 22 237.4 0 22 18 1 0.0 1 0 1 19 5 0.0 1 3 1 1 20 8 0.0 2 6 1 1 21 25 0.0 2 16 8 1 22 34 0.0 2 23 4 7 23 372 0.0 2 61 70 241 24 5245 0.0 2 1116 4032 97 25 213274 0.0 2 208470 4732 72 26 227 0.0 2 147 79 1 27 1 0.0 2 0 0 1 28 1 0.0 2 1 29 1 0.0 2 1 30 1 0.0 2 1 39 1 0.0 2 1 44 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O103_130_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O103_322_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O103_130_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O103_322_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.91 s (148 us/read; 0.41 M reads/minute). === Summary === Total read pairs processed: 6,168 Read 1 with adapter: 2,739 (44.4%) Read 2 with adapter: 2,740 (44.4%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 6,168 (100.0%) Total basepairs processed: 2,493,922 bp Read 1: 1,246,777 bp Read 2: 1,247,145 bp Total written (filtered): 2,360,692 bp (94.7%) Read 1: 1,180,261 bp Read 2: 1,180,431 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 1097 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 83.4% C: 8.6% G: 7.7% T: 0.4% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 63 96.4 0 63 4 1 24.1 0 1 24 10 0.0 2 6 4 25 1023 0.0 2 1007 16 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 1642 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 16 96.4 0 16 4 3 24.1 0 3 5 2 6.0 0 2 19 1 0.0 1 1 23 5 0.0 2 0 0 5 24 74 0.0 2 5 47 22 25 1538 0.0 2 1275 238 25 26 3 0.0 2 0 3 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 1227 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.9% C: 0.3% G: 8.1% T: 74.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 1 24.1 0 1 23 3 0.0 2 0 1 2 24 31 0.0 2 9 3 19 25 1191 0.0 2 993 173 25 26 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 1513 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 67 96.4 0 67 4 8 24.1 0 8 5 3 6.0 0 3 23 3 0.0 2 0 0 3 24 31 0.0 2 2 27 2 25 1392 0.0 2 1347 44 1 26 8 0.0 2 2 6 27 1 0.0 2 0 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O105_131_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O105_323_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O105_131_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O105_323_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.06 s (238 us/read; 0.25 M reads/minute). === Summary === Total read pairs processed: 255 Read 1 with adapter: 23 (9.0%) Read 2 with adapter: 22 (8.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 255 (100.0%) Total basepairs processed: 123,061 bp Read 1: 61,521 bp Read 2: 61,540 bp Total written (filtered): 122,220 bp (99.3%) Read 1: 61,100 bp Read 2: 61,120 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 7 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.3% C: 0.0% G: 85.7% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6 4.0 0 6 25 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 16 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 4.0 0 1 25 15 0.0 2 5 8 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 9 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.8% C: 0.0% G: 11.1% T: 11.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 9 0.0 2 1 6 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 13 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 4.0 0 4 4 1 1.0 0 1 5 1 0.2 0 1 24 1 0.0 2 0 1 25 6 0.0 2 6 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O12_132_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O12_324_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O12_132_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O12_324_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 19.25 s (163 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 117,750 Read 1 with adapter: 116,917 (99.3%) Read 2 with adapter: 116,932 (99.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 117,750 (100.0%) Total basepairs processed: 58,691,604 bp Read 1: 29,319,640 bp Read 2: 29,371,964 bp Total written (filtered): 52,855,954 bp (90.1%) Read 1: 26,402,840 bp Read 2: 26,453,114 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 411 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 92.9% C: 2.9% G: 3.4% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 15 1839.8 0 15 16 1 0.0 1 1 19 33 0.0 1 32 1 20 40 0.0 2 36 4 21 6 0.0 2 6 22 2 0.0 2 2 23 1 0.0 2 1 24 4 0.0 2 3 1 25 309 0.0 2 309 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 116506 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 1839.8 0 3 14 1 0.0 1 1 16 1 0.0 1 0 1 17 1 0.0 1 1 18 2 0.0 1 0 1 1 19 16 0.0 1 13 3 20 11 0.0 2 7 3 1 21 30 0.0 2 18 6 6 22 35 0.0 2 13 9 13 23 375 0.0 2 41 86 248 24 4258 0.0 2 739 3411 108 25 111655 0.0 2 107711 3874 70 26 113 0.0 2 72 41 27 1 0.0 2 1 29 2 0.0 2 2 30 1 0.0 2 1 37 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 356 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 8.7% C: 2.8% G: 37.6% T: 50.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 11 3 0.0 1 2 1 19 36 0.0 1 34 2 20 1 0.0 2 0 1 21 6 0.0 2 5 0 1 22 1 0.0 2 1 24 6 0.0 2 4 1 1 25 302 0.0 2 269 30 3 42 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 116576 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 17 1839.8 0 17 4 7 460.0 0 7 5 2 115.0 0 2 13 1 0.0 1 1 16 2 0.0 1 2 18 2 0.0 1 2 20 2 0.0 2 1 0 1 21 7 0.0 2 5 1 1 22 23 0.0 2 10 4 9 23 223 0.0 2 38 44 141 24 3175 0.0 2 568 2520 87 25 112988 0.0 2 109747 3162 79 26 118 0.0 2 74 42 2 27 1 0.0 2 0 1 28 2 0.0 2 2 29 4 0.0 2 3 1 31 1 0.0 2 1 39 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O13_133_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O13_325_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O13_133_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O13_325_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 21.15 s (155 us/read; 0.39 M reads/minute). === Summary === Total read pairs processed: 136,761 Read 1 with adapter: 136,633 (99.9%) Read 2 with adapter: 136,663 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 136,761 (100.0%) Total basepairs processed: 68,230,871 bp Read 1: 34,077,064 bp Read 2: 34,153,807 bp Total written (filtered): 61,414,866 bp (90.0%) Read 1: 30,671,001 bp Read 2: 30,743,865 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 778 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 98.8% C: 0.6% G: 0.3% T: 0.3% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 5 2136.9 0 5 19 245 0.0 1 234 11 20 408 0.0 2 390 17 1 21 57 0.0 2 56 1 22 5 0.0 2 5 23 1 0.0 2 0 1 25 57 0.0 2 57 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 135855 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 2136.9 0 3 4 1 534.2 0 1 11 1 0.0 1 1 14 1 0.0 1 0 1 16 2 0.0 1 2 18 1 0.0 1 0 1 19 19 0.0 1 9 10 20 11 0.0 2 5 5 1 21 15 0.0 2 10 3 2 22 56 0.0 2 29 9 18 23 394 0.0 2 43 95 256 24 4770 0.0 2 814 3862 94 25 130386 0.0 2 126561 3767 58 26 195 0.0 2 125 69 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 458 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.7% C: 7.4% G: 3.7% T: 88.2% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 19 355 0.0 1 345 10 20 32 0.0 2 20 10 2 21 14 0.0 2 12 2 22 1 0.0 2 1 25 54 0.0 2 51 2 1 38 1 0.0 2 1 42 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 136205 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 2136.9 0 2 17 1 0.0 1 0 1 18 2 0.0 1 1 1 20 7 0.0 2 2 4 1 21 15 0.0 2 9 3 3 22 25 0.0 2 12 5 8 23 261 0.0 2 30 44 187 24 3745 0.0 2 660 2981 104 25 131990 0.0 2 128048 3823 119 26 149 0.0 2 80 69 28 3 0.0 2 2 1 29 3 0.0 2 2 1 31 1 0.0 2 1 38 1 0.0 2 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O14_134_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O14_326_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O14_134_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O14_326_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 15.51 s (163 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 94,969 Read 1 with adapter: 94,850 (99.9%) Read 2 with adapter: 94,892 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 94,969 (100.0%) Total basepairs processed: 47,411,983 bp Read 1: 23,695,872 bp Read 2: 23,716,111 bp Total written (filtered): 42,677,666 bp (90.0%) Read 1: 21,330,130 bp Read 2: 21,347,536 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 117 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 94.9% C: 0.9% G: 2.6% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 1483.9 0 1 19 23 0.0 1 19 4 20 41 0.0 2 40 1 21 8 0.0 2 8 22 1 0.0 2 1 24 1 0.0 2 1 25 42 0.0 2 41 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 94733 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1483.9 0 1 11 1 0.0 1 0 1 13 2 0.0 1 2 15 1 0.0 1 1 16 1 0.0 1 1 18 2 0.0 1 1 1 19 6 0.0 1 3 3 20 14 0.0 2 7 3 4 21 19 0.0 2 11 5 3 22 32 0.0 2 10 8 14 23 382 0.0 2 46 81 255 24 4114 0.0 2 624 3365 125 25 90021 0.0 2 86234 3715 72 26 135 0.0 2 72 60 3 28 1 0.0 2 1 29 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 78 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 2.6% C: 9.0% G: 15.4% T: 73.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 35 0.0 1 35 20 4 0.0 2 4 21 3 0.0 2 3 24 2 0.0 2 2 25 32 0.0 2 30 2 26 2 0.0 2 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 94814 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1483.9 0 1 18 4 0.0 1 0 4 19 3 0.0 1 2 1 20 2 0.0 2 2 21 12 0.0 2 8 0 4 22 28 0.0 2 8 9 11 23 217 0.0 2 14 39 164 24 2983 0.0 2 473 2404 106 25 91455 0.0 2 88325 3027 103 26 102 0.0 2 75 26 1 27 2 0.0 2 1 1 28 2 0.0 2 2 29 1 0.0 2 1 39 2 0.0 2 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O16_135_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O16_327_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O16_135_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O16_327_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 22.97 s (139 us/read; 0.43 M reads/minute). === Summary === Total read pairs processed: 165,052 Read 1 with adapter: 164,843 (99.9%) Read 2 with adapter: 164,927 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 165,052 (100.0%) Total basepairs processed: 82,445,665 bp Read 1: 41,214,261 bp Read 2: 41,231,404 bp Total written (filtered): 74,216,859 bp (90.0%) Read 1: 37,102,453 bp Read 2: 37,114,406 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 72 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.8% C: 1.4% G: 0.0% T: 2.8% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 2 2578.9 0 2 19 9 0.0 1 9 20 18 0.0 2 16 2 21 8 0.0 2 8 22 2 0.0 2 2 24 1 0.0 2 1 25 31 0.0 2 31 26 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 164771 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 2578.9 0 1 9 1 0.6 0 1 11 1 0.0 1 1 12 1 0.0 1 0 1 14 2 0.0 1 2 15 2 0.0 1 2 16 2 0.0 1 0 2 17 1 0.0 1 1 18 6 0.0 1 4 2 19 18 0.0 1 14 4 20 23 0.0 2 10 9 4 21 45 0.0 2 27 7 11 22 62 0.0 2 22 17 23 23 659 0.0 2 81 144 434 24 7167 0.0 2 983 5965 219 25 156582 0.0 2 150360 6082 140 26 195 0.0 2 116 72 7 29 2 0.0 2 2 30 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 66 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 3.0% C: 31.8% G: 13.6% T: 51.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 15 0.0 1 14 1 20 8 0.0 2 6 2 21 7 0.0 2 7 22 1 0.0 2 1 25 34 0.0 2 32 2 42 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 164861 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 2578.9 0 2 4 1 644.7 0 1 16 1 0.0 1 1 17 2 0.0 1 2 18 1 0.0 1 1 19 5 0.0 1 0 5 20 6 0.0 2 5 0 1 21 20 0.0 2 14 5 1 22 44 0.0 2 17 9 18 23 395 0.0 2 47 69 279 24 5120 0.0 2 797 4155 168 25 159067 0.0 2 153370 5508 189 26 182 0.0 2 112 64 6 27 4 0.0 2 1 2 1 28 4 0.0 2 4 29 5 0.0 2 5 34 1 0.0 2 1 40 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O19_136_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O19_328_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O19_136_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O19_328_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 24.02 s (150 us/read; 0.40 M reads/minute). === Summary === Total read pairs processed: 160,187 Read 1 with adapter: 160,035 (99.9%) Read 2 with adapter: 160,073 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 160,187 (100.0%) Total basepairs processed: 79,958,629 bp Read 1: 39,968,529 bp Read 2: 39,990,100 bp Total written (filtered): 71,969,432 bp (90.0%) Read 1: 35,975,919 bp Read 2: 35,993,513 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 206 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.1% C: 2.4% G: 0.5% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 3 2502.9 0 3 19 1 0.0 1 1 20 5 0.0 2 5 21 2 0.0 2 2 23 2 0.0 2 2 24 3 0.0 2 2 1 25 190 0.0 2 190 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 159829 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 2502.9 0 2 6 1 39.1 0 1 8 2 2.4 0 2 14 3 0.0 1 1 2 15 3 0.0 1 2 1 16 3 0.0 1 1 2 17 3 0.0 1 3 18 6 0.0 1 6 19 12 0.0 1 10 1 1 20 17 0.0 2 9 6 2 21 27 0.0 2 15 9 3 22 66 0.0 2 22 14 30 23 584 0.0 2 65 105 414 24 6517 0.0 2 994 5351 172 25 152365 0.0 2 147073 5225 67 26 211 0.0 2 129 81 1 27 1 0.0 2 1 29 3 0.0 2 3 31 1 0.0 2 1 32 1 0.0 2 1 35 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 156 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 3.2% C: 13.5% G: 17.9% T: 65.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 1 0.0 1 1 20 1 0.0 2 1 22 1 0.0 2 1 25 136 0.0 2 131 3 2 28 14 0.0 2 14 44 1 0.0 2 1 45 2 0.0 2 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 159917 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 2502.9 0 2 4 1 625.7 0 1 15 1 0.0 1 1 18 1 0.0 1 1 19 2 0.0 1 2 20 3 0.0 2 2 1 21 19 0.0 2 9 7 3 22 41 0.0 2 17 11 13 23 352 0.0 2 47 65 240 24 4601 0.0 2 768 3710 123 25 154696 0.0 2 149594 4985 117 26 181 0.0 2 117 63 1 27 3 0.0 2 2 1 28 5 0.0 2 4 1 29 4 0.0 2 4 38 2 0.0 2 2 39 2 0.0 2 2 41 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O20_137_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O20_329_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O20_137_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O20_329_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 15.30 s (160 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 95,565 Read 1 with adapter: 95,426 (99.9%) Read 2 with adapter: 95,491 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 95,565 (100.0%) Total basepairs processed: 47,690,737 bp Read 1: 23,828,909 bp Read 2: 23,861,828 bp Total written (filtered): 42,926,790 bp (90.0%) Read 1: 21,448,544 bp Read 2: 21,478,246 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 101 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 97.0% C: 1.0% G: 0.0% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 3 1493.2 0 3 19 9 0.0 1 8 1 20 8 0.0 2 8 21 3 0.0 2 3 24 1 0.0 2 1 25 77 0.0 2 76 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 95325 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 12 1 0.0 1 1 14 1 0.0 1 1 15 1 0.0 1 1 17 5 0.0 1 3 2 18 2 0.0 1 1 1 19 4 0.0 1 4 20 7 0.0 2 5 2 21 24 0.0 2 14 4 6 22 55 0.0 2 16 18 21 23 384 0.0 2 36 73 275 24 4103 0.0 2 568 3373 162 25 90578 0.0 2 85245 5206 127 26 157 0.0 2 76 75 6 27 1 0.0 2 1 29 2 0.0 2 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 88 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 1.1% C: 10.2% G: 36.4% T: 52.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 9 0.0 1 9 20 4 0.0 2 3 1 21 2 0.0 2 2 24 2 0.0 2 2 25 69 0.0 2 67 2 28 1 0.0 2 1 44 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 95403 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1493.2 0 1 17 1 0.0 1 1 18 2 0.0 1 1 1 20 6 0.0 2 2 0 4 21 15 0.0 2 10 5 22 24 0.0 2 10 3 11 23 226 0.0 2 22 41 163 24 3093 0.0 2 485 2515 93 25 91924 0.0 2 88783 3031 110 26 107 0.0 2 59 45 3 27 1 0.0 2 1 28 1 0.0 2 1 29 2 0.0 2 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O21_138_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O21_330_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O21_138_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O21_330_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.53 s (193 us/read; 0.31 M reads/minute). === Summary === Total read pairs processed: 2,730 Read 1 with adapter: 1,011 (37.0%) Read 2 with adapter: 1,007 (36.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 2,730 (100.0%) Total basepairs processed: 1,332,265 bp Read 1: 665,687 bp Read 2: 666,578 bp Total written (filtered): 1,283,763 bp (96.4%) Read 1: 641,474 bp Read 2: 642,289 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 65 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.5% C: 7.7% G: 70.8% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 37 42.7 0 37 25 28 0.0 2 25 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 946 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 9 42.7 0 9 4 1 10.7 0 1 23 3 0.0 2 0 1 2 24 25 0.0 2 4 17 4 25 906 0.0 2 839 62 5 26 2 0.0 2 1 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 58 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 51.7% C: 0.0% G: 31.0% T: 17.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 42.7 0 1 24 3 0.0 2 0 0 3 25 51 0.0 2 29 16 6 26 1 0.0 2 0 0 1 28 1 0.0 2 1 74 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 949 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 36 42.7 0 36 4 3 10.7 0 3 5 2 2.7 0 2 22 1 0.0 2 0 0 1 23 2 0.0 2 1 1 24 19 0.0 2 4 15 25 880 0.0 2 858 22 26 5 0.0 2 1 4 27 1 0.0 2 0 0 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O22_139_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O22_331_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O22_139_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O22_331_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 30.70 s (159 us/read; 0.38 M reads/minute). === Summary === Total read pairs processed: 192,660 Read 1 with adapter: 192,412 (99.9%) Read 2 with adapter: 192,505 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 192,660 (100.0%) Total basepairs processed: 96,201,731 bp Read 1: 48,092,404 bp Read 2: 48,109,327 bp Total written (filtered): 86,596,273 bp (90.0%) Read 1: 43,292,441 bp Read 2: 43,303,832 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 41 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 87.8% C: 7.3% G: 2.4% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 3010.3 0 1 20 3 0.0 2 3 23 2 0.0 2 2 24 1 0.0 2 1 25 34 0.0 2 34 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 192371 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 3010.3 0 2 4 2 752.6 0 2 14 2 0.0 1 2 15 2 0.0 1 0 2 16 1 0.0 1 1 17 3 0.0 1 1 2 18 4 0.0 1 1 3 19 21 0.0 1 12 7 2 20 22 0.0 2 8 11 3 21 34 0.0 2 19 7 8 22 73 0.0 2 27 19 27 23 795 0.0 2 98 162 535 24 8208 0.0 2 1246 6692 270 25 182934 0.0 2 174591 8162 181 26 263 0.0 2 137 123 3 28 1 0.0 2 1 29 3 0.0 2 3 30 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 29 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 3.4% C: 31.0% G: 34.5% T: 31.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 22 1 0.0 2 1 23 3 0.0 2 3 25 24 0.0 2 23 1 43 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 192476 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 3010.3 0 2 5 1 188.1 0 1 14 1 0.0 1 1 15 3 0.0 1 2 1 17 1 0.0 1 1 18 2 0.0 1 1 1 19 3 0.0 1 1 2 20 9 0.0 2 4 5 21 18 0.0 2 12 2 4 22 70 0.0 2 32 10 28 23 431 0.0 2 49 75 307 24 6106 0.0 2 1016 4905 185 25 185578 0.0 2 178784 6592 202 26 233 0.0 2 139 87 7 27 5 0.0 2 3 2 28 4 0.0 2 4 29 8 0.0 2 8 39 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O23_140_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O23_332_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O23_140_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O23_332_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 10.53 s (162 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 64,840 Read 1 with adapter: 64,387 (99.3%) Read 2 with adapter: 64,406 (99.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 64,840 (100.0%) Total basepairs processed: 32,289,708 bp Read 1: 16,136,780 bp Read 2: 16,152,928 bp Total written (filtered): 29,075,670 bp (90.0%) Read 1: 14,530,750 bp Read 2: 14,544,920 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 935 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.4% C: 1.5% G: 1.6% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 3 1013.1 0 3 4 1 253.3 0 1 11 1 0.0 1 0 1 19 33 0.0 1 33 20 65 0.0 2 62 3 21 38 0.0 2 37 1 22 16 0.0 2 16 23 40 0.0 2 40 24 11 0.0 2 10 1 25 727 0.0 2 726 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 63452 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 1013.1 0 3 17 2 0.0 1 2 18 3 0.0 1 2 1 19 5 0.0 1 4 1 20 4 0.0 2 3 1 21 14 0.0 2 8 4 2 22 22 0.0 2 7 6 9 23 189 0.0 2 30 44 115 24 2158 0.0 2 428 1683 47 25 60971 0.0 2 59083 1858 30 26 81 0.0 2 44 33 4 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 850 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.2% C: 10.1% G: 25.5% T: 60.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 57 0.0 1 56 1 20 18 0.0 2 16 2 21 24 0.0 2 24 22 21 0.0 2 21 23 27 0.0 2 25 2 24 4 0.0 2 4 25 674 0.0 2 638 35 1 42 12 0.0 2 12 43 1 0.0 2 1 44 3 0.0 2 2 1 45 5 0.0 2 5 46 1 0.0 2 1 57 1 0.0 2 1 88 2 0.0 2 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 63556 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 8 1013.1 0 8 18 2 0.0 1 0 2 20 1 0.0 2 0 0 1 21 4 0.0 2 3 1 22 17 0.0 2 11 5 1 23 107 0.0 2 18 25 64 24 1664 0.0 2 308 1309 47 25 61674 0.0 2 59870 1758 46 26 77 0.0 2 53 24 29 1 0.0 2 1 33 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O30_141_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O30_333_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O30_141_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O30_333_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 15.77 s (179 us/read; 0.33 M reads/minute). === Summary === Total read pairs processed: 87,878 Read 1 with adapter: 87,693 (99.8%) Read 2 with adapter: 87,723 (99.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 87,878 (100.0%) Total basepairs processed: 43,767,675 bp Read 1: 21,876,886 bp Read 2: 21,890,789 bp Total written (filtered): 39,391,149 bp (90.0%) Read 1: 19,689,892 bp Read 2: 19,701,257 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 617 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 97.2% C: 1.5% G: 0.5% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 3 1373.1 0 3 19 39 0.0 1 37 2 20 56 0.0 2 56 21 3 0.0 2 3 23 2 0.0 2 2 24 2 0.0 2 2 25 511 0.0 2 510 1 89 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 87076 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1373.1 0 1 9 1 0.3 0 1 12 1 0.0 1 0 1 14 3 0.0 1 2 1 15 1 0.0 1 0 1 17 1 0.0 1 0 1 18 2 0.0 1 2 19 13 0.0 1 11 2 20 8 0.0 2 4 2 2 21 15 0.0 2 7 2 6 22 40 0.0 2 16 10 14 23 334 0.0 2 30 68 236 24 3867 0.0 2 565 3148 154 25 82646 0.0 2 78240 4299 107 26 140 0.0 2 68 69 3 27 2 0.0 2 1 0 1 33 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 544 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 1.8% C: 9.2% G: 62.9% T: 26.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 1373.1 0 1 19 52 0.0 1 52 20 2 0.0 2 2 21 4 0.0 2 3 1 23 3 0.0 2 3 24 4 0.0 2 3 0 1 25 473 0.0 2 464 7 2 29 1 0.0 2 0 1 42 1 0.0 2 1 45 2 0.0 2 2 88 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 87179 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1373.1 0 1 15 1 0.0 1 1 16 2 0.0 1 1 1 17 1 0.0 1 1 18 1 0.0 1 1 19 2 0.0 1 0 2 20 4 0.0 2 0 3 1 21 7 0.0 2 7 22 28 0.0 2 12 5 11 23 214 0.0 2 23 36 155 24 2783 0.0 2 439 2272 72 25 84033 0.0 2 80915 3028 90 26 98 0.0 2 59 39 28 1 0.0 2 0 0 1 29 2 0.0 2 1 1 39 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O34_142_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O34_334_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O34_142_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O34_334_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 13.79 s (163 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 84,366 Read 1 with adapter: 83,526 (99.0%) Read 2 with adapter: 83,545 (99.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 84,366 (100.0%) Total basepairs processed: 41,994,450 bp Read 1: 20,985,328 bp Read 2: 21,009,122 bp Total written (filtered): 37,826,476 bp (90.1%) Read 1: 18,902,373 bp Read 2: 18,924,103 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 511 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 90.8% C: 5.3% G: 3.5% T: 0.4% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 8 1318.2 0 8 19 72 0.0 1 70 2 20 151 0.0 2 144 7 21 9 0.0 2 8 1 22 2 0.0 2 2 24 4 0.0 2 3 1 25 265 0.0 2 265 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 83015 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 1318.2 0 2 15 1 0.0 1 1 16 3 0.0 1 1 2 18 1 0.0 1 1 19 8 0.0 1 6 2 20 9 0.0 2 7 1 1 21 14 0.0 2 9 1 4 22 24 0.0 2 10 3 11 23 252 0.0 2 29 53 170 24 3112 0.0 2 521 2511 80 25 79451 0.0 2 76568 2827 56 26 137 0.0 2 66 69 2 27 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 447 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 12.8% C: 0.9% G: 32.4% T: 53.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 138 0.0 1 136 2 20 5 0.0 2 2 3 21 6 0.0 2 4 2 24 9 0.0 2 0 2 7 25 289 0.0 2 244 38 7 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 83098 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 1318.2 0 4 4 3 329.6 0 3 18 1 0.0 1 1 20 3 0.0 2 2 0 1 21 4 0.0 2 3 1 22 17 0.0 2 12 4 1 23 170 0.0 2 21 41 108 24 2254 0.0 2 394 1793 67 25 80537 0.0 2 78101 2380 56 26 101 0.0 2 62 37 2 28 3 0.0 2 3 29 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O35_143_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O35_335_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O35_143_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O35_335_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 13.32 s (155 us/read; 0.39 M reads/minute). === Summary === Total read pairs processed: 85,796 Read 1 with adapter: 85,624 (99.8%) Read 2 with adapter: 85,659 (99.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 85,796 (100.0%) Total basepairs processed: 42,752,354 bp Read 1: 21,369,810 bp Read 2: 21,382,544 bp Total written (filtered): 38,477,954 bp (90.0%) Read 1: 19,233,780 bp Read 2: 19,244,174 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 379 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 97.9% C: 0.8% G: 0.5% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 4 1340.6 0 4 21 5 0.0 2 5 24 5 0.0 2 2 3 25 364 0.0 2 363 0 1 227 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 85245 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 4 2 335.1 0 2 11 4 0.0 1 3 1 12 1 0.0 1 0 1 15 1 0.0 1 1 16 2 0.0 1 1 1 18 3 0.0 1 3 19 7 0.0 1 5 2 20 9 0.0 2 2 3 4 21 9 0.0 2 5 2 2 22 37 0.0 2 12 11 14 23 340 0.0 2 36 71 233 24 3732 0.0 2 562 3066 104 25 80968 0.0 2 77137 3755 76 26 127 0.0 2 65 58 4 28 1 0.0 2 0 1 29 1 0.0 2 1 38 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 348 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.3% C: 8.6% G: 84.5% T: 6.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 4 1 335.1 0 1 14 1 0.0 1 1 20 2 0.0 2 1 1 21 5 0.0 2 5 24 4 0.0 2 2 2 25 332 0.0 2 326 6 57 1 0.0 2 1 88 2 0.0 2 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 85311 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 1340.6 0 3 17 1 0.0 1 0 1 18 1 0.0 1 1 19 2 0.0 1 1 1 20 4 0.0 2 1 3 21 6 0.0 2 3 1 2 22 26 0.0 2 10 4 12 23 218 0.0 2 22 33 163 24 2649 0.0 2 406 2168 75 25 82309 0.0 2 79373 2864 72 26 88 0.0 2 43 44 1 28 1 0.0 2 1 29 3 0.0 2 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O36_144_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O36_336_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O36_144_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O36_336_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.46 s (160 us/read; 0.38 M reads/minute). === Summary === Total read pairs processed: 2,867 Read 1 with adapter: 362 (12.6%) Read 2 with adapter: 365 (12.7%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 2,867 (100.0%) Total basepairs processed: 1,315,636 bp Read 1: 657,654 bp Read 2: 657,982 bp Total written (filtered): 1,300,190 bp (98.8%) Read 1: 649,941 bp Read 2: 650,249 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 262 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.0% C: 6.1% G: 17.9% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 47 44.8 0 47 4 1 11.2 0 1 25 214 0.0 2 211 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 100 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 10 44.8 0 10 4 2 11.2 0 2 6 1 0.7 0 1 24 2 0.0 2 0 1 1 25 84 0.0 2 34 45 5 26 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 246 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.7% C: 0.8% G: 1.6% T: 76.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 44.8 0 3 5 1 2.8 0 1 24 1 0.0 2 0 0 1 25 241 0.0 2 188 48 5 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 119 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 48 44.8 0 48 4 8 11.2 0 8 5 4 2.8 0 4 24 1 0.0 2 0 1 25 58 0.0 2 57 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O37_145_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O37_337_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O37_145_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O37_337_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.18 s (187 us/read; 0.32 M reads/minute). === Summary === Total read pairs processed: 953 Read 1 with adapter: 225 (23.6%) Read 2 with adapter: 210 (22.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 953 (100.0%) Total basepairs processed: 437,094 bp Read 1: 218,501 bp Read 2: 218,593 bp Total written (filtered): 427,208 bp (97.7%) Read 1: 213,538 bp Read 2: 213,670 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 105 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 73.3% C: 2.9% G: 23.8% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 25 14.9 0 25 4 1 3.7 0 1 24 1 0.0 2 1 25 78 0.0 2 78 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 120 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 14.9 0 3 4 1 3.7 0 1 24 3 0.0 2 0 3 25 113 0.0 2 90 20 3 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 83 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.6% C: 0.0% G: 2.4% T: 88.0% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 2 25 81 0.0 2 73 5 3 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 127 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 10 14.9 0 10 4 2 3.7 0 2 5 3 0.9 0 3 24 3 0.0 2 0 3 25 109 0.0 2 102 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O41_146_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O41_338_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O41_146_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O41_338_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.33 s (162 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 8,233 Read 1 with adapter: 447 (5.4%) Read 2 with adapter: 438 (5.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 8,233 (100.0%) Total basepairs processed: 4,024,219 bp Read 1: 2,011,749 bp Read 2: 2,012,470 bp Total written (filtered): 4,011,578 bp (99.7%) Read 1: 2,005,436 bp Read 2: 2,006,142 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 231 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 10.8% C: 6.1% G: 82.7% T: 0.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 176 128.6 0 176 4 1 32.2 0 1 24 1 0.0 2 1 25 53 0.0 2 50 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 216 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 38 128.6 0 38 4 5 32.2 0 5 6 1 2.0 0 1 24 8 0.0 2 0 5 3 25 164 0.0 2 67 92 5 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 172 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 61.0% C: 0.6% G: 35.5% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 128.6 0 3 4 1 32.2 0 1 6 1 2.0 0 1 24 4 0.0 2 0 2 2 25 163 0.0 2 70 87 6 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 266 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 179 128.6 0 179 4 19 32.2 0 19 5 8 8.0 0 8 9 1 0.0 0 0 1 24 1 0.0 2 0 1 25 56 0.0 2 54 2 26 2 0.0 2 0 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O43_147_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O43_339_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O43_147_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O43_339_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.97 s (160 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 6,040 Read 1 with adapter: 862 (14.3%) Read 2 with adapter: 823 (13.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 6,040 (100.0%) Total basepairs processed: 2,887,363 bp Read 1: 1,443,388 bp Read 2: 1,443,975 bp Total written (filtered): 2,852,488 bp (98.8%) Read 1: 1,425,889 bp Read 2: 1,426,599 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 288 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 24.7% C: 13.9% G: 59.4% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 152 94.4 0 152 4 3 23.6 0 3 23 1 0.0 2 0 0 1 24 3 0.0 2 1 1 1 25 129 0.0 2 126 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 574 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 26 94.4 0 26 4 1 23.6 0 1 6 1 1.5 0 1 22 1 0.0 2 1 23 1 0.0 2 0 1 24 25 0.0 2 3 19 3 25 516 0.0 2 414 88 14 26 3 0.0 2 1 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 225 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 39.1% C: 0.9% G: 28.0% T: 32.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 22 1 0.0 2 1 24 4 0.0 2 0 2 2 25 220 0.0 2 132 71 17 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 598 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 130 94.4 0 130 4 12 23.6 0 12 5 3 5.9 0 3 23 1 0.0 2 0 0 1 24 21 0.0 2 1 17 3 25 428 0.0 2 409 17 2 26 3 0.0 2 0 3 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O48_148_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O48_340_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O48_148_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O48_340_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.72 s (185 us/read; 0.32 M reads/minute). === Summary === Total read pairs processed: 3,904 Read 1 with adapter: 429 (11.0%) Read 2 with adapter: 426 (10.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 3,904 (100.0%) Total basepairs processed: 1,850,039 bp Read 1: 924,855 bp Read 2: 925,184 bp Total written (filtered): 1,832,641 bp (99.1%) Read 1: 916,156 bp Read 2: 916,485 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 241 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 59.8% C: 7.1% G: 33.2% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 80 61.0 0 80 24 3 0.0 2 2 1 25 158 0.0 2 157 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 188 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 10 61.0 0 10 4 2 15.2 0 2 24 3 0.0 2 0 2 1 25 171 0.0 2 110 58 3 26 2 0.0 2 1 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 195 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.7% C: 0.5% G: 5.1% T: 66.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 0 1 25 193 0.0 2 136 52 5 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 231 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 78 61.0 0 78 4 9 15.2 0 9 5 2 3.8 0 2 24 6 0.0 2 0 6 25 134 0.0 2 132 2 26 2 0.0 2 0 1 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O51_149_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O51_341_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O51_149_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O51_341_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.21 s (178 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 1,207 Read 1 with adapter: 150 (12.4%) Read 2 with adapter: 147 (12.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,207 (100.0%) Total basepairs processed: 564,765 bp Read 1: 282,319 bp Read 2: 282,446 bp Total written (filtered): 558,523 bp (98.9%) Read 1: 279,185 bp Read 2: 279,338 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 84 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 61.9% C: 10.7% G: 27.4% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 22 18.9 0 22 25 62 0.0 2 62 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 66 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 6 18.9 0 6 24 2 0.0 2 0 2 25 57 0.0 2 40 16 1 27 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 70 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.0% C: 0.0% G: 10.0% T: 70.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 70 0.0 2 55 15 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 77 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 23 18.9 0 23 5 3 1.2 0 3 24 1 0.0 2 1 25 50 0.0 2 47 3 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O6_150_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O6_342_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O6_150_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O6_342_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 26.46 s (147 us/read; 0.41 M reads/minute). === Summary === Total read pairs processed: 179,582 Read 1 with adapter: 179,390 (99.9%) Read 2 with adapter: 179,415 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 179,582 (100.0%) Total basepairs processed: 89,632,327 bp Read 1: 44,780,691 bp Read 2: 44,851,636 bp Total written (filtered): 80,678,833 bp (90.0%) Read 1: 40,305,952 bp Read 2: 40,372,881 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 115 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 88.7% C: 2.6% G: 5.2% T: 3.5% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 8 2806.0 0 8 19 10 0.0 1 10 20 41 0.0 2 41 21 3 0.0 2 3 22 1 0.0 2 1 25 52 0.0 2 51 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 179275 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 2806.0 0 4 7 1 11.0 0 1 8 1 2.7 0 1 10 1 0.2 1 1 12 3 0.0 1 0 3 13 1 0.0 1 0 1 14 3 0.0 1 2 1 15 1 0.0 1 0 1 16 2 0.0 1 1 1 17 4 0.0 1 3 1 18 5 0.0 1 4 1 19 29 0.0 1 20 9 20 21 0.0 2 5 8 8 21 31 0.0 2 23 5 3 22 61 0.0 2 16 15 30 23 686 0.0 2 66 151 469 24 7548 0.0 2 1150 6159 239 25 170608 0.0 2 163106 7345 157 26 263 0.0 2 158 101 4 27 1 0.0 2 0 0 1 28 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 77 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.1% C: 9.1% G: 36.4% T: 45.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 2806.0 0 1 19 22 0.0 1 21 1 20 3 0.0 2 2 1 21 3 0.0 2 2 1 23 1 0.0 2 0 0 1 24 2 0.0 2 0 1 1 25 44 0.0 2 40 4 88 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 179338 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 2806.0 0 3 4 1 701.5 0 1 5 1 175.4 0 1 15 1 0.0 1 1 16 2 0.0 1 1 1 17 1 0.0 1 1 18 1 0.0 1 0 1 19 3 0.0 1 2 0 1 20 8 0.0 2 6 2 21 21 0.0 2 12 4 5 22 52 0.0 2 34 9 9 23 432 0.0 2 57 76 299 24 5425 0.0 2 808 4425 192 25 173184 0.0 2 165900 6985 299 26 190 0.0 2 116 68 6 27 4 0.0 2 2 0 2 28 5 0.0 2 5 29 3 0.0 2 3 39 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O61_151_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O61_343_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O61_151_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O61_343_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 2.52 s (157 us/read; 0.38 M reads/minute). === Summary === Total read pairs processed: 16,081 Read 1 with adapter: 1,119 (7.0%) Read 2 with adapter: 1,100 (6.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 16,081 (100.0%) Total basepairs processed: 7,756,764 bp Read 1: 3,876,826 bp Read 2: 3,879,938 bp Total written (filtered): 7,713,040 bp (99.4%) Read 1: 3,854,951 bp Read 2: 3,858,089 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 587 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 51.3% C: 7.0% G: 41.6% T: 0.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 236 251.3 0 236 4 2 62.8 0 2 24 2 0.0 2 2 25 347 0.0 2 342 5 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 532 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 33 251.3 0 33 4 4 62.8 0 4 5 2 15.7 0 2 22 1 0.0 2 0 0 1 24 12 0.0 2 0 8 4 25 479 0.0 2 374 92 13 26 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 415 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.4% C: 0.7% G: 9.9% T: 66.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 251.3 0 2 4 1 62.8 0 1 5 2 15.7 0 2 6 1 3.9 0 1 24 6 0.0 2 1 1 4 25 403 0.0 2 309 80 14 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 685 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 221 251.3 0 221 4 24 62.8 0 24 5 7 15.7 0 7 23 2 0.0 2 1 0 1 24 15 0.0 2 1 13 1 25 412 0.0 2 401 11 26 4 0.0 2 0 4 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O66_152_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O66_344_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O66_152_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O66_344_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 9.09 s (168 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 54,241 Read 1 with adapter: 54,181 (99.9%) Read 2 with adapter: 54,186 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 54,241 (100.0%) Total basepairs processed: 27,067,546 bp Read 1: 13,533,598 bp Read 2: 13,533,948 bp Total written (filtered): 24,363,280 bp (90.0%) Read 1: 12,182,063 bp Read 2: 12,181,217 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 173 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 96.5% C: 1.2% G: 1.2% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 3 847.5 0 3 20 3 0.0 2 3 25 166 0.0 2 166 26 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 54008 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 847.5 0 1 17 1 0.0 1 1 18 2 0.0 1 2 19 5 0.0 1 3 2 20 6 0.0 2 3 2 1 21 10 0.0 2 8 0 2 22 22 0.0 2 9 6 7 23 212 0.0 2 17 44 151 24 2418 0.0 2 386 1940 92 25 51192 0.0 2 48123 2999 70 26 138 0.0 2 38 95 5 29 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 100 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 2.0% C: 7.0% G: 6.0% T: 85.0% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 1 25 91 0.0 2 86 4 1 27 1 0.0 2 1 28 7 0.0 2 7 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 54086 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 847.5 0 1 4 1 211.9 0 1 18 2 0.0 1 1 1 20 3 0.0 2 2 1 21 9 0.0 2 7 1 1 22 15 0.0 2 5 2 8 23 109 0.0 2 13 18 78 24 1632 0.0 2 248 1346 38 25 52256 0.0 2 50364 1837 55 26 56 0.0 2 37 18 1 27 1 0.0 2 1 29 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O70_153_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O70_345_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O70_153_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O70_345_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 12.01 s (175 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 68,753 Read 1 with adapter: 68,663 (99.9%) Read 2 with adapter: 68,696 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 68,753 (100.0%) Total basepairs processed: 34,305,314 bp Read 1: 17,153,000 bp Read 2: 17,152,314 bp Total written (filtered): 30,877,324 bp (90.0%) Read 1: 15,440,200 bp Read 2: 15,437,124 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 728 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 98.8% C: 0.7% G: 0.1% T: 0.4% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 3 1074.3 0 3 20 2 0.0 2 2 21 3 0.0 2 3 22 2 0.0 2 2 23 1 0.0 2 1 24 3 0.0 2 2 1 25 714 0.0 2 713 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 67935 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 11 1 0.0 1 1 12 1 0.0 1 0 1 14 1 0.0 1 1 17 2 0.0 1 1 1 19 9 0.0 1 6 3 20 7 0.0 2 7 21 9 0.0 2 4 1 4 22 30 0.0 2 9 5 16 23 282 0.0 2 25 65 192 24 3029 0.0 2 405 2468 156 25 64387 0.0 2 60012 4268 107 26 174 0.0 2 48 120 6 29 3 0.0 2 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 467 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.4% C: 1.9% G: 6.0% T: 91.6% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 20 3 0.0 2 3 21 2 0.0 2 2 24 3 0.0 2 1 2 25 371 0.0 2 361 10 26 1 0.0 2 1 27 9 0.0 2 8 1 28 70 0.0 2 69 1 29 6 0.0 2 5 1 42 1 0.0 2 1 88 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 68229 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 1074.3 0 2 13 2 0.0 1 1 1 19 2 0.0 1 1 1 20 3 0.0 2 2 1 21 5 0.0 2 5 22 20 0.0 2 12 0 8 23 134 0.0 2 8 29 97 24 2169 0.0 2 317 1778 74 25 65820 0.0 2 63210 2525 85 26 68 0.0 2 43 22 3 28 1 0.0 2 1 29 1 0.0 2 1 31 1 0.0 2 1 39 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O71_154_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O71_346_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O71_154_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O71_346_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 7.89 s (170 us/read; 0.35 M reads/minute). === Summary === Total read pairs processed: 46,442 Read 1 with adapter: 46,395 (99.9%) Read 2 with adapter: 46,408 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 46,442 (100.0%) Total basepairs processed: 23,179,545 bp Read 1: 11,591,131 bp Read 2: 11,588,414 bp Total written (filtered): 20,863,717 bp (90.0%) Read 1: 10,433,838 bp Read 2: 10,429,879 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 67 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 98.5% C: 1.5% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 21 4 0.0 2 4 24 4 0.0 2 3 1 25 58 0.0 2 58 29 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 46328 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 725.7 0 1 11 1 0.0 1 1 14 1 0.0 1 1 15 1 0.0 1 0 1 17 1 0.0 1 0 1 18 1 0.0 1 0 1 19 4 0.0 1 3 1 20 3 0.0 2 1 2 21 6 0.0 2 2 2 2 22 22 0.0 2 13 2 7 23 194 0.0 2 13 35 146 24 2065 0.0 2 283 1707 75 25 43940 0.0 2 41515 2363 62 26 88 0.0 2 35 52 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 46 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 2.2% C: 17.4% G: 4.3% T: 76.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 20 1 0.0 2 1 21 1 0.0 2 1 22 1 0.0 2 1 24 1 0.0 2 1 25 40 0.0 2 38 2 27 1 0.0 2 1 28 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 46362 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 725.7 0 1 18 1 0.0 1 1 20 3 0.0 2 1 2 21 2 0.0 2 2 22 13 0.0 2 5 4 4 23 113 0.0 2 7 18 88 24 1418 0.0 2 238 1137 43 25 44761 0.0 2 43052 1651 58 26 46 0.0 2 24 21 1 27 1 0.0 2 0 0 1 28 1 0.0 2 1 29 1 0.0 2 1 48 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O72_155_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O72_347_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O72_155_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O72_347_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 13.22 s (167 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 78,965 Read 1 with adapter: 78,850 (99.9%) Read 2 with adapter: 78,878 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 78,965 (100.0%) Total basepairs processed: 39,414,365 bp Read 1: 19,702,013 bp Read 2: 19,712,352 bp Total written (filtered): 35,478,576 bp (90.0%) Read 1: 17,735,253 bp Read 2: 17,743,323 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 331 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 97.6% C: 0.0% G: 0.3% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 7 1233.8 0 7 20 1 0.0 2 1 23 1 0.0 2 1 24 1 0.0 2 1 25 319 0.0 2 319 26 1 0.0 2 1 29 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 78519 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 1233.8 0 2 5 1 77.1 0 1 8 1 1.2 0 1 11 2 0.0 1 2 13 1 0.0 1 1 14 1 0.0 1 1 16 1 0.0 1 0 1 17 2 0.0 1 1 1 18 2 0.0 1 2 19 8 0.0 1 8 20 9 0.0 2 2 5 2 21 11 0.0 2 4 4 3 22 38 0.0 2 17 5 16 23 310 0.0 2 32 62 216 24 3426 0.0 2 511 2779 136 25 74569 0.0 2 70475 3990 104 26 135 0.0 2 49 83 3 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 279 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 3.6% G: 1.8% T: 94.6% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 23 1 0.0 2 1 25 235 0.0 2 231 3 1 26 2 0.0 2 2 27 3 0.0 2 3 28 34 0.0 2 33 1 29 4 0.0 2 2 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 78599 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 15 1 0.0 1 0 1 17 2 0.0 1 2 18 1 0.0 1 0 1 19 3 0.0 1 1 2 20 6 0.0 2 4 2 21 6 0.0 2 3 3 22 25 0.0 2 8 12 5 23 208 0.0 2 16 35 157 24 2560 0.0 2 402 2087 71 25 75705 0.0 2 73271 2393 41 26 74 0.0 2 39 35 27 1 0.0 2 1 28 2 0.0 2 2 29 4 0.0 2 4 38 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O73_156_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O73_348_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O73_156_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O73_348_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 24.16 s (165 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 146,568 Read 1 with adapter: 146,351 (99.9%) Read 2 with adapter: 146,428 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 146,568 (100.0%) Total basepairs processed: 73,170,133 bp Read 1: 36,577,794 bp Read 2: 36,592,339 bp Total written (filtered): 65,863,640 bp (90.0%) Read 1: 32,926,869 bp Read 2: 32,936,771 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 1230 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 98.5% C: 0.5% G: 0.2% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 11 2290.1 0 11 4 1 572.5 0 1 20 1 0.0 2 1 24 2 0.0 2 1 1 25 1211 0.0 2 1207 3 1 26 1 0.0 2 1 28 2 0.0 2 2 29 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 145121 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 2290.1 0 3 4 1 572.5 0 1 8 1 2.2 0 1 9 1 0.6 0 1 13 1 0.0 1 0 1 16 3 0.0 1 2 1 17 3 0.0 1 1 2 18 1 0.0 1 1 19 16 0.0 1 10 6 20 18 0.0 2 8 7 3 21 28 0.0 2 18 6 4 22 65 0.0 2 27 14 24 23 531 0.0 2 53 96 382 24 6105 0.0 2 924 4974 207 25 138102 0.0 2 131257 6698 147 26 236 0.0 2 106 125 5 28 1 0.0 2 1 29 5 0.0 2 5 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 1007 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 3.1% G: 3.3% T: 93.6% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 23 2 0.0 2 1 1 24 12 0.0 2 9 3 25 910 0.0 2 904 6 26 3 0.0 2 3 27 2 0.0 2 2 28 65 0.0 2 64 1 29 12 0.0 2 8 4 31 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 145421 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 2290.1 0 4 18 5 0.0 1 5 19 4 0.0 1 3 1 20 6 0.0 2 3 1 2 21 24 0.0 2 17 5 2 22 32 0.0 2 16 4 12 23 321 0.0 2 54 51 216 24 4627 0.0 2 739 3773 115 25 140213 0.0 2 135634 4475 104 26 174 0.0 2 101 71 2 27 3 0.0 2 3 28 2 0.0 2 2 29 1 0.0 2 1 30 1 0.0 2 1 38 1 0.0 2 1 39 2 0.0 2 2 55 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O74_157_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O74_349_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O74_157_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O74_349_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 9.72 s (175 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 55,697 Read 1 with adapter: 55,619 (99.9%) Read 2 with adapter: 55,650 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 55,697 (100.0%) Total basepairs processed: 27,809,002 bp Read 1: 13,903,124 bp Read 2: 13,905,878 bp Total written (filtered): 25,033,082 bp (90.0%) Read 1: 12,516,107 bp Read 2: 12,516,975 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 201 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 98.0% C: 0.5% G: 0.0% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 3 870.3 0 3 19 39 0.0 1 39 20 60 0.0 2 55 5 21 6 0.0 2 5 1 22 2 0.0 2 2 25 90 0.0 2 90 28 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 55418 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 4 1 217.6 0 1 8 1 0.8 0 1 14 1 0.0 1 0 1 17 1 0.0 1 1 18 1 0.0 1 0 1 19 4 0.0 1 1 3 20 4 0.0 2 2 1 1 21 7 0.0 2 6 1 22 19 0.0 2 6 4 9 23 237 0.0 2 16 45 176 24 2270 0.0 2 303 1897 70 25 52770 0.0 2 50283 2429 58 26 100 0.0 2 45 52 3 27 1 0.0 2 0 0 1 29 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 124 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 4.0% G: 4.8% T: 91.1% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 19 50 0.0 1 50 20 2 0.0 2 1 1 21 3 0.0 2 3 25 62 0.0 2 61 0 1 27 2 0.0 2 2 28 5 0.0 2 5 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 55526 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 870.3 0 2 13 1 0.0 1 0 1 18 1 0.0 1 1 20 4 0.0 2 3 0 1 21 6 0.0 2 3 3 22 12 0.0 2 4 3 5 23 130 0.0 2 14 26 90 24 1708 0.0 2 274 1396 38 25 53601 0.0 2 51962 1602 37 26 58 0.0 2 31 26 1 27 1 0.0 2 0 1 28 1 0.0 2 1 29 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O75_158_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O75_350_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O75_158_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O75_350_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.09 s (265 us/read; 0.23 M reads/minute). === Summary === Total read pairs processed: 329 Read 1 with adapter: 209 (63.5%) Read 2 with adapter: 206 (62.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 329 (100.0%) Total basepairs processed: 160,063 bp Read 1: 80,035 bp Read 2: 80,028 bp Total written (filtered): 149,919 bp (93.7%) Read 1: 74,970 bp Read 2: 74,949 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 10 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.0% C: 20.0% G: 20.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 5.1 0 2 4 1 1.3 0 1 25 7 0.0 2 7 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 199 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 5.1 0 4 23 1 0.0 2 0 0 1 24 5 0.0 2 1 4 25 189 0.0 2 173 16 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 9 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 22.2% C: 0.0% G: 11.1% T: 66.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 1 25 8 0.0 2 7 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 197 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 5.1 0 2 4 1 1.3 0 1 23 1 0.0 2 0 0 1 24 4 0.0 2 0 4 25 188 0.0 2 181 7 26 1 0.0 2 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O76_159_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O76_351_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O76_159_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O76_351_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 8.76 s (157 us/read; 0.38 M reads/minute). === Summary === Total read pairs processed: 55,703 Read 1 with adapter: 55,616 (99.8%) Read 2 with adapter: 55,623 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 55,703 (100.0%) Total basepairs processed: 27,798,744 bp Read 1: 13,891,059 bp Read 2: 13,907,685 bp Total written (filtered): 25,022,968 bp (90.0%) Read 1: 12,503,689 bp Read 2: 12,519,279 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 38 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.3% C: 2.6% G: 13.2% T: 7.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 870.4 0 4 12 1 0.0 1 1 21 1 0.0 2 1 25 32 0.0 2 32 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 55578 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 15 1 0.0 1 1 17 1 0.0 1 0 1 18 1 0.0 1 1 19 7 0.0 1 3 3 1 20 9 0.0 2 1 5 3 21 11 0.0 2 7 3 1 22 20 0.0 2 5 5 10 23 239 0.0 2 20 49 170 24 2342 0.0 2 325 1936 81 25 52840 0.0 2 50099 2672 69 26 105 0.0 2 42 62 1 27 1 0.0 2 0 0 1 29 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 26 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 7.7% C: 15.4% G: 15.4% T: 61.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 25 0.0 2 24 1 28 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 55597 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 870.4 0 2 4 1 217.6 0 1 19 2 0.0 1 2 20 3 0.0 2 2 0 1 21 4 0.0 2 3 0 1 22 11 0.0 2 5 3 3 23 128 0.0 2 9 21 98 24 1853 0.0 2 285 1521 47 25 53531 0.0 2 51775 1711 45 26 60 0.0 2 34 22 4 29 1 0.0 2 0 1 39 1 0.0 2 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O77_160_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O77_352_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O77_160_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O77_352_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.11 s (217 us/read; 0.28 M reads/minute). === Summary === Total read pairs processed: 510 Read 1 with adapter: 85 (16.7%) Read 2 with adapter: 82 (16.1%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 510 (100.0%) Total basepairs processed: 248,023 bp Read 1: 123,990 bp Read 2: 124,033 bp Total written (filtered): 244,358 bp (98.5%) Read 1: 122,155 bp Read 2: 122,203 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 13 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 7.7% C: 0.0% G: 92.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 12 8.0 0 12 25 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 72 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 8.0 0 1 24 4 0.0 2 0 3 1 25 67 0.0 2 53 10 4 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 13 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.9% C: 0.0% G: 0.0% T: 23.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 2 0 0 1 25 12 0.0 2 2 6 4 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 69 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 7 8.0 0 7 4 2 2.0 0 2 5 1 0.5 0 1 23 1 0.0 2 0 0 1 24 1 0.0 2 0 1 25 57 0.0 2 55 2 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O80_161_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O80_353_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O80_161_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O80_353_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.06 s (248 us/read; 0.24 M reads/minute). === Summary === Total read pairs processed: 244 Read 1 with adapter: 116 (47.5%) Read 2 with adapter: 108 (44.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 244 (100.0%) Total basepairs processed: 107,231 bp Read 1: 53,589 bp Read 2: 53,642 bp Total written (filtered): 101,816 bp (95.0%) Read 1: 50,872 bp Read 2: 50,944 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 30 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.7% C: 3.3% G: 20.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6 3.8 0 6 24 1 0.0 2 0 1 25 23 0.0 2 23 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 86 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 3.8 0 1 4 1 1.0 0 1 24 7 0.0 2 0 7 25 77 0.0 2 60 17 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 29 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 31.0% C: 0.0% G: 0.0% T: 69.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 29 0.0 2 20 9 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 79 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 1 25 77 0.0 2 75 2 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O84_162_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O84_354_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O84_162_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O84_354_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 16.08 s (169 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 95,222 Read 1 with adapter: 95,030 (99.8%) Read 2 with adapter: 95,105 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 95,222 (100.0%) Total basepairs processed: 47,498,612 bp Read 1: 23,738,142 bp Read 2: 23,760,470 bp Total written (filtered): 42,754,892 bp (90.0%) Read 1: 21,367,952 bp Read 2: 21,386,940 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 199 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 94.5% C: 2.0% G: 2.0% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 2 1487.8 0 2 4 1 372.0 0 1 19 69 0.0 1 67 2 20 74 0.0 2 70 4 21 8 0.0 2 8 22 1 0.0 2 1 24 1 0.0 2 0 1 25 43 0.0 2 43 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 94831 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 9 2 0.4 0 2 14 1 0.0 1 1 16 2 0.0 1 2 18 1 0.0 1 1 19 10 0.0 1 7 3 20 7 0.0 2 5 2 21 18 0.0 2 14 4 22 21 0.0 2 9 4 8 23 323 0.0 2 41 68 214 24 3851 0.0 2 625 3044 182 25 90479 0.0 2 85013 5326 140 26 114 0.0 2 61 49 4 27 1 0.0 2 0 0 1 29 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 163 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.7% C: 3.7% G: 2.5% T: 87.1% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 1487.8 0 1 19 101 0.0 1 101 20 4 0.0 2 2 2 21 3 0.0 2 3 22 1 0.0 2 1 24 2 0.0 2 0 1 1 25 51 0.0 2 39 9 3 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 94942 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1487.8 0 1 5 1 93.0 0 1 18 3 0.0 1 1 2 20 2 0.0 2 0 2 21 10 0.0 2 4 4 2 22 30 0.0 2 13 9 8 23 237 0.0 2 33 31 173 24 2878 0.0 2 460 2352 66 25 91670 0.0 2 88917 2686 67 26 105 0.0 2 67 36 2 27 2 0.0 2 1 0 1 28 2 0.0 2 2 35 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O85_163_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O85_355_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O85_163_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O85_355_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 27.21 s (160 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 169,564 Read 1 with adapter: 169,338 (99.9%) Read 2 with adapter: 169,450 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 169,564 (100.0%) Total basepairs processed: 84,592,084 bp Read 1: 42,290,074 bp Read 2: 42,302,010 bp Total written (filtered): 76,137,435 bp (90.0%) Read 1: 38,065,486 bp Read 2: 38,071,949 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 304 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.1% C: 1.0% G: 3.0% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 4 2649.4 0 4 20 1 0.0 2 1 21 3 0.0 2 3 22 1 0.0 2 1 24 4 0.0 2 2 2 25 290 0.0 2 287 3 27 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 169034 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 2649.4 0 4 4 1 662.4 0 1 12 1 0.0 1 0 1 15 1 0.0 1 1 16 3 0.0 1 2 1 17 4 0.0 1 4 18 2 0.0 1 0 2 19 13 0.0 1 9 3 1 20 20 0.0 2 12 5 3 21 29 0.0 2 17 7 5 22 84 0.0 2 33 11 40 23 628 0.0 2 58 124 446 24 7085 0.0 2 1018 5576 491 25 160825 0.0 2 146420 13978 427 26 331 0.0 2 131 193 7 27 1 0.0 2 0 0 1 28 1 0.0 2 0 1 29 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 252 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.8% C: 9.9% G: 8.7% T: 80.6% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 20 3 0.0 2 3 21 1 0.0 2 1 22 1 0.0 2 1 24 2 0.0 2 1 1 25 232 0.0 2 227 5 26 1 0.0 2 1 27 3 0.0 2 3 28 9 0.0 2 9 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 169198 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 2649.4 0 4 12 1 0.0 1 0 1 15 1 0.0 1 1 17 2 0.0 1 2 18 2 0.0 1 1 1 19 3 0.0 1 2 1 20 3 0.0 2 2 0 1 21 18 0.0 2 13 4 1 22 33 0.0 2 16 7 10 23 387 0.0 2 43 61 283 24 5325 0.0 2 851 4356 118 25 163211 0.0 2 158244 4890 77 26 197 0.0 2 115 76 6 27 4 0.0 2 4 28 2 0.0 2 2 29 3 0.0 2 3 33 1 0.0 2 1 39 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O86_164_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O86_356_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O86_164_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O86_356_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.12 s (244 us/read; 0.25 M reads/minute). === Summary === Total read pairs processed: 507 Read 1 with adapter: 189 (37.3%) Read 2 with adapter: 194 (38.3%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 507 (100.0%) Total basepairs processed: 232,923 bp Read 1: 116,369 bp Read 2: 116,554 bp Total written (filtered): 223,757 bp (96.1%) Read 1: 111,804 bp Read 2: 111,953 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 31 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 48.4% C: 32.3% G: 19.4% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 7.9 0 3 25 28 0.0 2 28 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 158 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 4 7.9 0 4 24 7 0.0 2 0 4 3 25 146 0.0 2 127 15 4 26 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 44 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.1% C: 2.3% G: 13.6% T: 50.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 7.9 0 1 24 3 0.0 2 1 0 2 25 40 0.0 2 25 12 3 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 150 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 5 7.9 0 5 4 4 2.0 0 4 5 1 0.5 0 1 23 2 0.0 2 1 1 24 6 0.0 2 0 6 25 132 0.0 2 129 3 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O90_165_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O90_357_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O90_165_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O90_357_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.25 s (175 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 1,406 Read 1 with adapter: 1,291 (91.8%) Read 2 with adapter: 1,293 (92.0%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 1,406 (100.0%) Total basepairs processed: 689,087 bp Read 1: 344,427 bp Read 2: 344,660 bp Total written (filtered): 624,751 bp (90.7%) Read 1: 312,266 bp Read 2: 312,485 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 25 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 84.0% C: 12.0% G: 4.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 22.0 0 1 4 1 5.5 0 1 25 23 0.0 2 23 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 1266 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 22.0 0 1 23 2 0.0 2 0 0 2 24 45 0.0 2 5 37 3 25 1218 0.0 2 1159 59 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 26 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.4% C: 0.0% G: 3.8% T: 80.8% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 25 25 0.0 2 21 4 42 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 1267 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 5 22.0 0 5 21 1 0.0 2 1 22 2 0.0 2 0 1 1 23 6 0.0 2 0 3 3 24 36 0.0 2 8 27 1 25 1216 0.0 2 1196 20 26 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O93_166_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O93_358_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O93_166_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O93_358_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 18.87 s (168 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 112,389 Read 1 with adapter: 112,215 (99.8%) Read 2 with adapter: 112,277 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 112,389 (100.0%) Total basepairs processed: 56,079,963 bp Read 1: 28,045,305 bp Read 2: 28,034,658 bp Total written (filtered): 50,477,117 bp (90.0%) Read 1: 25,245,439 bp Read 2: 25,231,678 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 69 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.8% C: 11.6% G: 4.3% T: 7.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 1756.1 0 2 4 1 439.0 0 1 19 2 0.0 1 2 23 1 0.0 2 1 24 1 0.0 2 0 1 25 61 0.0 2 60 1 62 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 112146 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1756.1 0 1 13 1 0.0 1 0 1 14 1 0.0 1 0 1 15 4 0.0 1 2 2 17 2 0.0 1 2 18 1 0.0 1 1 19 10 0.0 1 7 2 1 20 8 0.0 2 4 3 1 21 22 0.0 2 10 5 7 22 39 0.0 2 21 4 14 23 396 0.0 2 52 80 264 24 4446 0.0 2 697 3520 229 25 107031 0.0 2 99730 7123 178 26 183 0.0 2 87 90 6 27 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 58 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.5% C: 12.1% G: 17.2% T: 55.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 2 0.0 1 2 23 1 0.0 2 1 24 1 0.0 2 0 1 25 54 0.0 2 47 5 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 112219 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 1756.1 0 3 4 1 439.0 0 1 15 1 0.0 1 1 18 1 0.0 1 0 1 19 2 0.0 1 0 2 20 5 0.0 2 3 1 1 21 13 0.0 2 12 1 22 21 0.0 2 9 5 7 23 237 0.0 2 27 44 166 24 3353 0.0 2 516 2751 86 25 108439 0.0 2 105237 3149 53 26 140 0.0 2 86 52 2 28 1 0.0 2 1 29 1 0.0 2 1 31 1 0.0 2 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O97_167_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O97_359_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O97_167_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O97_359_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.12 s (164 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 750 Read 1 with adapter: 461 (61.5%) Read 2 with adapter: 462 (61.6%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 750 (100.0%) Total basepairs processed: 362,022 bp Read 1: 180,815 bp Read 2: 181,207 bp Total written (filtered): 339,186 bp (93.7%) Read 1: 169,388 bp Read 2: 169,798 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 12 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 41.7% C: 16.7% G: 41.7% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 11.7 0 2 25 10 0.0 2 10 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 449 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 11.7 0 2 24 11 0.0 2 2 9 25 435 0.0 2 402 30 3 26 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 25 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.0% C: 0.0% G: 16.0% T: 28.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 25 0.0 2 10 12 3 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 437 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 6 11.7 0 6 23 1 0.0 2 0 0 1 24 7 0.0 2 2 5 25 423 0.0 2 408 15 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedBP1_168_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedBP1_360_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedBP1_168_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedBP1_360_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 19.93 s (166 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 120,390 Read 1 with adapter: 120,131 (99.8%) Read 2 with adapter: 120,201 (99.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 120,390 (100.0%) Total basepairs processed: 60,077,174 bp Read 1: 30,058,765 bp Read 2: 30,018,409 bp Total written (filtered): 54,080,158 bp (90.0%) Read 1: 27,062,154 bp Read 2: 27,018,004 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 65 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 67.7% C: 6.2% G: 18.5% T: 7.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 1881.1 0 5 4 1 470.3 0 1 20 24 0.0 2 24 21 7 0.0 2 7 22 2 0.0 2 2 23 1 0.0 2 1 25 25 0.0 2 23 2 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 120066 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 1881.1 0 3 12 3 0.0 1 2 1 14 1 0.0 1 1 15 2 0.0 1 1 1 16 1 0.0 1 0 1 17 1 0.0 1 1 18 4 0.0 1 2 1 1 19 15 0.0 1 8 6 1 20 13 0.0 2 5 5 3 21 16 0.0 2 10 4 2 22 53 0.0 2 20 11 22 23 438 0.0 2 45 83 310 24 5177 0.0 2 757 4152 268 25 114129 0.0 2 105817 8096 216 26 202 0.0 2 94 104 4 27 3 0.0 2 1 0 2 28 1 0.0 2 0 1 29 3 0.0 2 3 37 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 43 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.0% C: 23.3% G: 34.9% T: 27.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 1881.1 0 1 18 1 0.0 1 1 20 2 0.0 2 2 21 5 0.0 2 5 23 1 0.0 2 1 24 1 0.0 2 1 25 31 0.0 2 28 2 1 30 1 0.0 2 0 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 120158 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 1881.1 0 3 4 1 470.3 0 1 5 2 117.6 0 2 17 1 0.0 1 0 1 18 1 0.0 1 1 19 2 0.0 1 2 20 3 0.0 2 2 0 1 21 11 0.0 2 8 2 1 22 39 0.0 2 13 11 15 23 300 0.0 2 32 42 226 24 3801 0.0 2 561 3137 103 25 115863 0.0 2 112136 3659 68 26 121 0.0 2 66 52 3 27 1 0.0 2 0 1 28 5 0.0 2 2 1 2 29 1 0.0 2 1 31 2 0.0 2 2 39 1 0.0 2 0 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedBP3_170_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedBP3_362_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedBP3_170_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedBP3_362_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 21.19 s (176 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 120,635 Read 1 with adapter: 120,384 (99.8%) Read 2 with adapter: 120,458 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 120,635 (100.0%) Total basepairs processed: 60,201,282 bp Read 1: 30,111,123 bp Read 2: 30,090,159 bp Total written (filtered): 54,191,708 bp (90.0%) Read 1: 27,108,316 bp Read 2: 27,083,392 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 61 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 67.2% C: 8.2% G: 13.1% T: 11.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 1884.9 0 5 20 7 0.0 2 7 21 3 0.0 2 3 22 1 0.0 2 1 23 5 0.0 2 5 24 3 0.0 2 2 1 25 37 0.0 2 37 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 120323 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 6 1884.9 0 6 10 1 0.1 1 1 12 1 0.0 1 0 1 15 1 0.0 1 0 1 16 1 0.0 1 1 17 3 0.0 1 2 1 18 3 0.0 1 3 19 6 0.0 1 4 1 1 20 24 0.0 2 11 6 7 21 25 0.0 2 13 4 8 22 47 0.0 2 16 10 21 23 485 0.0 2 48 98 339 24 5214 0.0 2 699 4205 310 25 114327 0.0 2 105473 8641 213 26 177 0.0 2 89 84 4 29 2 0.0 2 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 63 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.3% C: 54.0% G: 15.9% T: 15.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 1884.9 0 1 4 1 471.2 0 1 16 1 0.0 1 1 18 2 0.0 1 2 20 3 0.0 2 3 22 2 0.0 2 2 23 4 0.0 2 3 1 24 2 0.0 2 1 1 25 46 0.0 2 40 6 32 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 120395 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 1884.9 0 3 4 1 471.2 0 1 14 1 0.0 1 1 15 1 0.0 1 0 1 18 2 0.0 1 1 1 19 2 0.0 1 0 2 20 3 0.0 2 3 21 14 0.0 2 9 3 2 22 31 0.0 2 10 7 14 23 295 0.0 2 44 33 218 24 3860 0.0 2 625 3147 88 25 116044 0.0 2 112265 3705 74 26 133 0.0 2 80 48 5 27 2 0.0 2 1 0 1 28 1 0.0 2 1 29 1 0.0 2 1 36 1 0.0 2 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedBp2_169_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedBp2_361_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedBp2_169_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedBp2_361_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 20.85 s (176 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 118,484 Read 1 with adapter: 118,186 (99.7%) Read 2 with adapter: 118,271 (99.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 118,484 (100.0%) Total basepairs processed: 59,124,618 bp Read 1: 29,582,177 bp Read 2: 29,542,441 bp Total written (filtered): 53,224,200 bp (90.0%) Read 1: 26,634,206 bp Read 2: 26,589,994 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 69 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 68.1% C: 5.8% G: 7.2% T: 18.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6 1851.3 0 6 21 1 0.0 2 1 22 2 0.0 2 2 23 3 0.0 2 2 1 25 56 0.0 2 55 1 32 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 118117 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 1851.3 0 3 10 1 0.1 1 1 12 1 0.0 1 1 14 3 0.0 1 3 15 2 0.0 1 1 1 17 2 0.0 1 1 1 18 4 0.0 1 1 3 19 13 0.0 1 7 5 1 20 11 0.0 2 5 5 1 21 28 0.0 2 16 4 8 22 48 0.0 2 12 12 24 23 483 0.0 2 39 97 347 24 5217 0.0 2 729 4194 294 25 112101 0.0 2 103194 8652 255 26 192 0.0 2 92 92 8 27 2 0.0 2 0 0 2 29 3 0.0 2 2 1 30 1 0.0 2 1 31 2 0.0 2 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 66 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 3.0% C: 62.1% G: 15.2% T: 19.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 12 1 0.0 1 0 1 18 2 0.0 1 2 22 4 0.0 2 4 24 3 0.0 2 1 1 1 25 53 0.0 2 52 1 26 1 0.0 2 0 0 1 35 1 0.0 2 1 64 1 0.0 2 0 0 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 118205 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1851.3 0 1 12 1 0.0 1 1 14 1 0.0 1 1 18 2 0.0 1 2 19 1 0.0 1 0 1 20 8 0.0 2 3 5 21 18 0.0 2 11 3 4 22 37 0.0 2 11 3 23 23 267 0.0 2 29 51 187 24 3705 0.0 2 541 3074 90 25 114017 0.0 2 110159 3772 86 26 135 0.0 2 76 58 1 27 2 0.0 2 1 1 28 6 0.0 2 6 29 1 0.0 2 1 32 1 0.0 2 1 34 1 0.0 2 1 40 1 0.0 2 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedK1_171_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedK1_363_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedK1_171_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedK1_363_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.95 s (169 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 11,537 Read 1 with adapter: 11,513 (99.8%) Read 2 with adapter: 11,528 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 11,537 (100.0%) Total basepairs processed: 5,757,583 bp Read 1: 2,880,643 bp Read 2: 2,876,940 bp Total written (filtered): 5,182,633 bp (90.0%) Read 1: 2,593,512 bp Read 2: 2,589,121 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 15 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 93.3% C: 0.0% G: 6.7% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 20 7 0.0 2 7 25 8 0.0 2 8 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 11498 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 180.3 0 1 18 1 0.0 1 1 19 1 0.0 1 0 0 1 20 2 0.0 2 1 1 21 2 0.0 2 1 1 22 7 0.0 2 2 2 3 23 46 0.0 2 3 12 31 24 516 0.0 2 75 421 20 25 10899 0.0 2 10293 593 13 26 23 0.0 2 14 9 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 8 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 12.5% G: 12.5% T: 75.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 8 0.0 2 8 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 11520 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 22 3 0.0 2 0 2 1 23 23 0.0 2 1 5 17 24 340 0.0 2 51 281 8 25 11140 0.0 2 10740 388 12 26 14 0.0 2 5 8 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedK2_172_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedK2_364_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedK2_172_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedK2_364_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 4.65 s (169 us/read; 0.35 M reads/minute). === Summary === Total read pairs processed: 27,468 Read 1 with adapter: 27,409 (99.8%) Read 2 with adapter: 27,415 (99.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 27,468 (100.0%) Total basepairs processed: 13,705,231 bp Read 1: 6,856,079 bp Read 2: 6,849,152 bp Total written (filtered): 12,337,408 bp (90.0%) Read 1: 6,172,635 bp Read 2: 6,164,773 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 58 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 82.8% C: 8.6% G: 0.0% T: 8.6% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 2 429.2 0 2 19 1 0.0 1 1 20 7 0.0 2 7 22 3 0.0 2 3 23 7 0.0 2 7 25 38 0.0 2 38 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 27351 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 429.2 0 3 13 1 0.0 1 0 1 19 7 0.0 1 4 3 20 2 0.0 2 1 0 1 21 8 0.0 2 5 2 1 22 12 0.0 2 7 1 4 23 122 0.0 2 9 21 92 24 1301 0.0 2 165 1093 43 25 25839 0.0 2 24291 1506 42 26 52 0.0 2 26 23 3 27 2 0.0 2 0 2 28 1 0.0 2 0 1 36 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 46 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.3% C: 52.2% G: 15.2% T: 28.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 1 0.0 1 1 21 1 0.0 2 1 22 3 0.0 2 2 1 23 4 0.0 2 4 24 1 0.0 2 0 0 1 25 35 0.0 2 34 1 30 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 27369 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 19 2 0.0 1 1 1 21 5 0.0 2 2 3 22 10 0.0 2 3 2 5 23 67 0.0 2 14 12 41 24 801 0.0 2 111 665 25 25 26460 0.0 2 25413 1026 21 26 24 0.0 2 15 9 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedK3_173_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedK3_365_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedK3_173_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedK3_365_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.36 s (175 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 7,746 Read 1 with adapter: 7,740 (99.9%) Read 2 with adapter: 7,741 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 7,746 (100.0%) Total basepairs processed: 3,866,156 bp Read 1: 1,932,889 bp Read 2: 1,933,267 bp Total written (filtered): 3,479,938 bp (90.0%) Read 1: 1,739,890 bp Read 2: 1,740,048 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 15 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 93.3% C: 0.0% G: 0.0% T: 6.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 121.0 0 1 20 3 0.0 2 3 21 1 0.0 2 1 22 1 0.0 2 1 23 3 0.0 2 3 25 6 0.0 2 6 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 7725 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 19 1 0.0 1 0 1 20 2 0.0 2 1 1 21 1 0.0 2 1 23 43 0.0 2 3 8 32 24 357 0.0 2 60 276 21 25 7310 0.0 2 6767 527 16 26 10 0.0 2 2 7 1 27 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 14 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 7.1% C: 64.3% G: 14.3% T: 14.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 1 0.0 1 1 22 2 0.0 2 2 23 2 0.0 2 2 24 1 0.0 2 0 0 1 25 7 0.0 2 7 31 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 7727 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 21 2 0.0 2 2 22 1 0.0 2 0 0 1 23 15 0.0 2 3 2 10 24 267 0.0 2 36 226 5 25 7431 0.0 2 7183 242 6 26 10 0.0 2 7 3 28 1 0.0 2 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedL1_174_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedL1_366_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedL1_174_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedL1_366_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 22.36 s (171 us/read; 0.35 M reads/minute). === Summary === Total read pairs processed: 131,126 Read 1 with adapter: 130,924 (99.8%) Read 2 with adapter: 131,028 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 131,126 (100.0%) Total basepairs processed: 65,449,940 bp Read 1: 32,716,229 bp Read 2: 32,733,711 bp Total written (filtered): 58,913,439 bp (90.0%) Read 1: 29,450,291 bp Read 2: 29,463,148 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 136 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 86.8% C: 7.4% G: 0.7% T: 5.1% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 2048.8 0 1 20 2 0.0 2 2 21 10 0.0 2 10 22 13 0.0 2 13 23 32 0.0 2 31 1 24 2 0.0 2 2 25 76 0.0 2 75 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 130788 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 2048.8 0 1 9 1 0.5 0 1 12 1 0.0 1 1 13 1 0.0 1 0 1 14 1 0.0 1 1 16 1 0.0 1 0 1 17 3 0.0 1 1 2 18 7 0.0 1 5 2 19 10 0.0 1 8 1 1 20 12 0.0 2 7 5 21 20 0.0 2 10 5 5 22 63 0.0 2 20 19 24 23 537 0.0 2 68 123 346 24 5606 0.0 2 767 4497 342 25 124298 0.0 2 113331 10674 293 26 219 0.0 2 98 111 10 27 3 0.0 2 1 0 2 28 1 0.0 2 1 29 3 0.0 2 1 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 122 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.1% C: 50.8% G: 4.9% T: 40.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 20 5 0.0 2 5 21 6 0.0 2 6 22 8 0.0 2 8 23 30 0.0 2 30 24 3 0.0 2 1 0 2 25 66 0.0 2 63 3 30 1 0.0 2 0 1 40 1 0.0 2 1 42 2 0.0 2 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 130906 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 4 1 512.2 0 1 16 1 0.0 1 0 1 18 3 0.0 1 2 1 19 3 0.0 1 2 1 20 3 0.0 2 2 1 21 12 0.0 2 4 7 1 22 38 0.0 2 20 6 12 23 353 0.0 2 49 63 241 24 4290 0.0 2 641 3524 125 25 126056 0.0 2 121999 3985 72 26 137 0.0 2 90 45 2 27 1 0.0 2 1 28 4 0.0 2 4 29 2 0.0 2 2 39 2 0.0 2 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedL2_175_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedL2_367_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedL2_175_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedL2_367_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 32.25 s (174 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 185,304 Read 1 with adapter: 184,992 (99.8%) Read 2 with adapter: 185,111 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 185,304 (100.0%) Total basepairs processed: 92,452,011 bp Read 1: 46,216,341 bp Read 2: 46,235,670 bp Total written (filtered): 83,216,700 bp (90.0%) Read 1: 41,601,598 bp Read 2: 41,615,102 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 228 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 92.1% C: 4.4% G: 1.3% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 2 2895.4 0 2 4 2 723.8 0 2 20 7 0.0 2 7 21 16 0.0 2 16 22 37 0.0 2 36 1 23 63 0.0 2 63 24 7 0.0 2 6 1 25 94 0.0 2 94 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 184764 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 2895.4 0 1 4 1 723.8 0 1 12 1 0.0 1 1 14 1 0.0 1 1 15 3 0.0 1 2 1 17 6 0.0 1 1 5 18 7 0.0 1 4 3 19 23 0.0 1 10 12 1 20 15 0.0 2 7 6 2 21 26 0.0 2 14 7 5 22 83 0.0 2 34 16 33 23 705 0.0 2 57 128 520 24 7814 0.0 2 1099 6213 502 25 175728 0.0 2 159717 15533 478 26 347 0.0 2 120 216 11 27 1 0.0 2 0 0 1 28 1 0.0 2 0 1 29 1 0.0 2 0 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 215 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 2.3% C: 55.3% G: 7.4% T: 34.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 2895.4 0 1 20 4 0.0 2 3 1 21 14 0.0 2 14 22 27 0.0 2 27 23 62 0.0 2 60 2 24 7 0.0 2 4 1 2 25 98 0.0 2 93 3 2 42 1 0.0 2 1 44 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 184896 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 7 2895.4 0 7 4 1 723.8 0 1 13 1 0.0 1 1 14 1 0.0 1 0 1 15 1 0.0 1 1 16 1 0.0 1 0 1 19 4 0.0 1 2 2 20 6 0.0 2 3 1 2 21 21 0.0 2 10 4 7 22 55 0.0 2 25 12 18 23 422 0.0 2 57 66 299 24 5836 0.0 2 922 4762 152 25 178329 0.0 2 172389 5808 132 26 203 0.0 2 110 88 5 27 2 0.0 2 2 29 2 0.0 2 2 30 1 0.0 2 1 35 1 0.0 2 1 37 1 0.0 2 0 0 1 50 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedL3_176_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedL3_368_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedL3_176_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedL3_368_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 0.07 s (258 us/read; 0.23 M reads/minute). === Summary === Total read pairs processed: 281 Read 1 with adapter: 96 (34.2%) Read 2 with adapter: 96 (34.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 281 (100.0%) Total basepairs processed: 125,387 bp Read 1: 62,670 bp Read 2: 62,717 bp Total written (filtered): 120,687 bp (96.3%) Read 1: 60,320 bp Read 2: 60,367 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 31 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 87.1% C: 9.7% G: 3.2% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 4.4 0 1 25 30 0.0 2 30 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 65 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 4.4 0 1 24 6 0.0 2 2 3 1 25 58 0.0 2 53 5 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 30 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 13.3% C: 3.3% G: 0.0% T: 83.3% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 24 2 0.0 2 1 0 1 25 28 0.0 2 25 3 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 66 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 4.4 0 2 24 4 0.0 2 0 4 25 60 0.0 2 59 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedLU1_177_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedLU1_369_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedLU1_177_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedLU1_369_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 2.10 s (168 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 12,468 Read 1 with adapter: 12,442 (99.8%) Read 2 with adapter: 12,455 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 12,468 (100.0%) Total basepairs processed: 6,218,867 bp Read 1: 3,110,779 bp Read 2: 3,108,088 bp Total written (filtered): 5,597,682 bp (90.0%) Read 1: 2,800,455 bp Read 2: 2,797,227 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 40 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 97.5% C: 0.0% G: 2.5% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 19 1 0.0 1 1 20 8 0.0 2 8 21 3 0.0 2 3 24 1 0.0 2 0 1 25 27 0.0 2 26 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 12402 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 194.8 0 1 19 1 0.0 1 1 21 5 0.0 2 4 0 1 22 6 0.0 2 0 3 3 23 52 0.0 2 3 8 41 24 533 0.0 2 70 410 53 25 11771 0.0 2 10373 1355 43 26 32 0.0 2 9 23 29 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 25 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.0% C: 88.0% G: 4.0% T: 4.0% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 19 2 0.0 1 2 21 2 0.0 2 2 24 1 0.0 2 1 25 20 0.0 2 18 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 12430 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 0 1 22 3 0.0 2 2 0 1 23 32 0.0 2 3 5 24 24 440 0.0 2 77 349 14 25 11930 0.0 2 11544 376 10 26 24 0.0 2 14 9 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedLU2_178_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedLU2_370_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedLU2_178_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedLU2_370_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 1.25 s (161 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 7,787 Read 1 with adapter: 7,765 (99.7%) Read 2 with adapter: 7,775 (99.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 7,787 (100.0%) Total basepairs processed: 3,885,602 bp Read 1: 1,943,268 bp Read 2: 1,942,334 bp Total written (filtered): 3,497,778 bp (90.0%) Read 1: 1,749,549 bp Read 2: 1,748,229 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 17 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 121.7 0 1 21 3 0.0 2 3 25 13 0.0 2 13 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 7748 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 19 1 0.0 1 1 20 1 0.0 2 0 1 21 1 0.0 2 0 1 22 1 0.0 2 0 1 23 27 0.0 2 3 1 23 24 329 0.0 2 40 261 28 25 7360 0.0 2 6426 905 29 26 27 0.0 2 6 21 27 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 14 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 78.6% G: 0.0% T: 21.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 1 0.0 1 1 20 2 0.0 2 2 25 10 0.0 2 10 56 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 7761 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 1 22 3 0.0 2 0 1 2 23 25 0.0 2 6 3 16 24 226 0.0 2 31 187 8 25 7502 0.0 2 7248 244 10 26 4 0.0 2 2 1 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedLU3_179_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedLU3_371_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedLU3_179_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedLU3_371_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 3.29 s (161 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 20,509 Read 1 with adapter: 20,461 (99.8%) Read 2 with adapter: 20,481 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 20,509 (100.0%) Total basepairs processed: 10,231,772 bp Read 1: 5,117,013 bp Read 2: 5,114,759 bp Total written (filtered): 9,210,171 bp (90.0%) Read 1: 4,606,647 bp Read 2: 4,603,524 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 21 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.2% C: 0.0% G: 0.0% T: 4.8% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 320.5 0 1 20 1 0.0 2 1 21 1 0.0 2 1 25 18 0.0 2 18 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 20440 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 12 1 0.0 1 0 1 18 1 0.0 1 0 1 19 2 0.0 1 2 20 3 0.0 2 3 21 8 0.0 2 3 1 4 22 5 0.0 2 1 1 3 23 83 0.0 2 6 21 56 24 927 0.0 2 136 742 49 25 19351 0.0 2 17543 1754 54 26 59 0.0 2 19 38 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 26 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.5% C: 57.7% G: 23.1% T: 7.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 16 1 0.0 1 1 22 1 0.0 2 1 25 23 0.0 2 19 4 34 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 20455 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 18 2 0.0 1 2 19 1 0.0 1 1 20 2 0.0 2 2 21 2 0.0 2 2 22 7 0.0 2 4 1 2 23 35 0.0 2 6 2 27 24 680 0.0 2 109 548 23 25 19704 0.0 2 19034 656 14 26 22 0.0 2 10 12 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedS1_180_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedS1_372_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedS1_180_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedS1_372_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 10.64 s (163 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 65,423 Read 1 with adapter: 65,217 (99.7%) Read 2 with adapter: 65,263 (99.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 65,423 (100.0%) Total basepairs processed: 32,662,975 bp Read 1: 16,333,603 bp Read 2: 16,329,372 bp Total written (filtered): 29,407,660 bp (90.0%) Read 1: 14,707,378 bp Read 2: 14,700,282 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 162 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 90.1% C: 3.1% G: 6.2% T: 0.6% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 4 1022.2 0 4 4 1 255.6 0 1 20 72 0.0 2 72 21 6 0.0 2 6 22 8 0.0 2 8 23 20 0.0 2 20 25 51 0.0 2 50 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 65055 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 5 1022.2 0 5 13 1 0.0 1 0 1 14 1 0.0 1 1 16 1 0.0 1 1 17 1 0.0 1 0 1 18 2 0.0 1 0 2 19 11 0.0 1 7 3 1 20 7 0.0 2 2 3 2 21 10 0.0 2 2 3 5 22 21 0.0 2 6 7 8 23 306 0.0 2 35 64 207 24 2781 0.0 2 428 2245 108 25 61798 0.0 2 58530 3187 81 26 107 0.0 2 47 56 4 28 1 0.0 2 1 29 2 0.0 2 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 93 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.4% C: 77.4% G: 8.6% T: 8.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 2 0.0 1 2 21 3 0.0 2 3 22 8 0.0 2 8 23 21 0.0 2 20 0 1 24 1 0.0 2 0 1 25 58 0.0 2 56 1 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 65170 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 1022.2 0 2 4 1 255.6 0 1 5 1 63.9 0 1 17 1 0.0 1 1 20 3 0.0 2 1 2 21 7 0.0 2 2 4 1 22 22 0.0 2 13 3 6 23 126 0.0 2 11 23 92 24 2036 0.0 2 299 1686 51 25 62888 0.0 2 60890 1954 44 26 77 0.0 2 45 32 27 2 0.0 2 0 2 28 2 0.0 2 2 29 1 0.0 2 1 30 1 0.0 2 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedS2_181_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedS2_373_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedS2_181_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedS2_373_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 23.92 s (172 us/read; 0.35 M reads/minute). === Summary === Total read pairs processed: 139,170 Read 1 with adapter: 138,895 (99.8%) Read 2 with adapter: 138,940 (99.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 139,170 (100.0%) Total basepairs processed: 69,466,759 bp Read 1: 34,743,691 bp Read 2: 34,723,068 bp Total written (filtered): 62,533,505 bp (90.0%) Read 1: 31,278,757 bp Read 2: 31,254,748 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 894 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 97.8% C: 0.6% G: 0.3% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 2 2174.5 0 2 20 18 0.0 2 18 21 4 0.0 2 4 22 6 0.0 2 6 23 16 0.0 2 16 24 5 0.0 2 3 2 25 828 0.0 2 825 3 35 14 0.0 2 14 36 1 0.0 2 0 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 138001 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 2174.5 0 2 11 1 0.0 1 1 13 2 0.0 1 1 1 14 2 0.0 1 2 15 1 0.0 1 0 1 17 3 0.0 1 2 1 18 2 0.0 1 1 1 19 11 0.0 1 7 4 20 14 0.0 2 6 6 2 21 27 0.0 2 15 6 6 22 57 0.0 2 24 14 19 23 580 0.0 2 54 117 409 24 5994 0.0 2 852 4888 254 25 131020 0.0 2 123281 7546 193 26 280 0.0 2 127 147 6 27 2 0.0 2 0 1 1 29 2 0.0 2 2 67 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 920 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.1% C: 97.8% G: 1.2% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 2 2174.5 0 2 5 1 135.9 0 1 20 1 0.0 2 1 21 2 0.0 2 2 22 6 0.0 2 6 23 19 0.0 2 19 24 10 0.0 2 3 5 2 25 869 0.0 2 867 2 26 2 0.0 2 2 31 1 0.0 2 1 35 5 0.0 2 5 38 2 0.0 2 1 0 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 138020 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 3 2174.5 0 3 4 1 543.6 0 1 17 1 0.0 1 1 18 2 0.0 1 1 1 19 4 0.0 1 2 2 20 4 0.0 2 2 0 2 21 16 0.0 2 12 3 1 22 27 0.0 2 11 7 9 23 367 0.0 2 44 59 264 24 4333 0.0 2 700 3532 101 25 133073 0.0 2 128522 4462 89 26 176 0.0 2 98 71 7 27 8 0.0 2 0 1 7 28 1 0.0 2 1 29 1 0.0 2 1 39 2 0.0 2 2 42 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedS3_182_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedS3_374_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedS3_182_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedS3_374_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 7.12 s (149 us/read; 0.40 M reads/minute). === Summary === Total read pairs processed: 47,931 Read 1 with adapter: 47,831 (99.8%) Read 2 with adapter: 47,857 (99.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 47,931 (100.0%) Total basepairs processed: 23,930,750 bp Read 1: 11,967,613 bp Read 2: 11,963,137 bp Total written (filtered): 21,542,896 bp (90.0%) Read 1: 10,774,411 bp Read 2: 10,768,485 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 90 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 84.4% C: 8.9% G: 3.3% T: 3.3% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 748.9 0 1 19 1 0.0 1 1 20 15 0.0 2 15 22 1 0.0 2 1 23 6 0.0 2 6 24 3 0.0 2 2 1 25 61 0.0 2 60 1 35 1 0.0 2 1 89 1 0.0 2 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 47741 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 748.9 0 2 17 2 0.0 1 0 2 18 2 0.0 1 1 1 19 4 0.0 1 3 1 20 4 0.0 2 1 3 21 8 0.0 2 4 2 2 22 23 0.0 2 7 4 12 23 191 0.0 2 14 49 128 24 2010 0.0 2 282 1656 72 25 45423 0.0 2 43154 2214 55 26 69 0.0 2 40 28 1 28 1 0.0 2 1 31 1 0.0 2 1 32 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 65 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.6% C: 81.5% G: 9.2% T: 4.6% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 1 22 4 0.0 2 4 23 3 0.0 2 3 24 2 0.0 2 0 1 1 25 54 0.0 2 52 2 41 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 47792 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 2 748.9 0 2 4 1 187.2 0 1 13 1 0.0 1 0 1 19 1 0.0 1 1 20 4 0.0 2 1 3 21 5 0.0 2 3 1 1 22 10 0.0 2 5 3 2 23 101 0.0 2 14 12 75 24 1493 0.0 2 224 1232 37 25 46112 0.0 2 44559 1523 30 26 58 0.0 2 37 19 2 28 1 0.0 2 1 29 2 0.0 2 2 39 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedSU1_183_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedSU1_375_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedSU1_183_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedSU1_375_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 3.87 s (169 us/read; 0.35 M reads/minute). === Summary === Total read pairs processed: 22,853 Read 1 with adapter: 22,811 (99.8%) Read 2 with adapter: 22,829 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 22,853 (100.0%) Total basepairs processed: 11,405,885 bp Read 1: 5,702,734 bp Read 2: 5,703,151 bp Total written (filtered): 10,267,457 bp (90.0%) Read 1: 5,133,992 bp Read 2: 5,133,465 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 60 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 96.7% C: 1.7% G: 1.7% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 19 5 0.0 1 5 20 9 0.0 2 9 21 5 0.0 2 5 22 8 0.0 2 8 23 14 0.0 2 14 24 1 0.0 2 1 25 18 0.0 2 17 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 22751 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 357.1 0 1 9 1 0.1 0 1 13 1 0.0 1 0 1 15 1 0.0 1 0 1 18 1 0.0 1 1 19 2 0.0 1 1 1 20 1 0.0 2 0 0 1 21 5 0.0 2 4 0 1 22 11 0.0 2 3 0 8 23 115 0.0 2 13 21 81 24 1075 0.0 2 138 877 60 25 21480 0.0 2 19753 1683 44 26 57 0.0 2 14 43 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 54 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.6% C: 75.9% G: 1.9% T: 16.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 6 0.0 1 5 1 20 1 0.0 2 1 21 2 0.0 2 2 22 9 0.0 2 8 1 23 17 0.0 2 16 1 24 1 0.0 2 1 25 17 0.0 2 14 3 31 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 22775 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 19 2 0.0 1 1 1 20 2 0.0 2 1 1 21 2 0.0 2 1 1 22 9 0.0 2 6 2 1 23 56 0.0 2 2 9 45 24 784 0.0 2 132 636 16 25 21901 0.0 2 21125 758 18 26 19 0.0 2 14 5 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedSU2_184_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedSU2_376_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedSU2_184_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedSU2_376_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 4.14 s (167 us/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 24,819 Read 1 with adapter: 24,783 (99.9%) Read 2 with adapter: 24,799 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 24,819 (100.0%) Total basepairs processed: 12,388,453 bp Read 1: 6,193,812 bp Read 2: 6,194,641 bp Total written (filtered): 11,151,440 bp (90.0%) Read 1: 5,575,769 bp Read 2: 5,575,671 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 35 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 88.6% C: 0.0% G: 2.9% T: 8.6% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 387.8 0 1 19 4 0.0 1 3 1 20 1 0.0 2 1 21 1 0.0 2 1 22 1 0.0 2 1 23 15 0.0 2 15 24 1 0.0 2 0 1 25 11 0.0 2 11 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 24748 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 14 2 0.0 1 1 1 17 1 0.0 1 0 1 18 2 0.0 1 1 1 19 2 0.0 1 2 20 4 0.0 2 1 0 3 21 6 0.0 2 3 3 22 17 0.0 2 8 1 8 23 105 0.0 2 6 20 79 24 1151 0.0 2 151 935 65 25 23395 0.0 2 21605 1743 47 26 60 0.0 2 19 39 2 27 2 0.0 2 0 2 30 1 0.0 2 0 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 33 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.1% C: 66.7% G: 9.1% T: 15.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 19 2 0.0 1 2 20 1 0.0 2 1 22 4 0.0 2 4 23 14 0.0 2 14 25 12 0.0 2 11 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 24766 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 387.8 0 1 19 1 0.0 1 0 1 21 2 0.0 2 1 1 22 7 0.0 2 1 2 4 23 63 0.0 2 4 9 50 24 799 0.0 2 121 646 32 25 23859 0.0 2 22800 1021 38 26 34 0.0 2 19 14 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedSU3_185_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedSU3_377_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedSU3_185_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedSU3_377_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 6.09 s (173 us/read; 0.35 M reads/minute). === Summary === Total read pairs processed: 35,157 Read 1 with adapter: 35,102 (99.8%) Read 2 with adapter: 35,125 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 35,157 (100.0%) Total basepairs processed: 17,551,087 bp Read 1: 8,774,680 bp Read 2: 8,776,407 bp Total written (filtered): 15,798,840 bp (90.0%) Read 1: 7,899,183 bp Read 2: 7,899,657 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 44 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.5% C: 0.0% G: 4.5% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 3 1 549.3 0 1 4 1 137.3 0 1 20 1 0.0 2 1 21 2 0.0 2 2 22 2 0.0 2 2 23 4 0.0 2 4 25 33 0.0 2 33 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 35058 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 9 1 0.1 0 1 14 1 0.0 1 1 15 1 0.0 1 1 17 2 0.0 1 2 18 1 0.0 1 0 1 19 4 0.0 1 3 0 1 20 1 0.0 2 1 21 8 0.0 2 2 2 4 22 14 0.0 2 3 2 9 23 155 0.0 2 11 26 118 24 1583 0.0 2 234 1278 71 25 33214 0.0 2 31281 1879 54 26 73 0.0 2 32 40 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 41 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 90.2% G: 0.0% T: 9.8% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence could be incomplete at its 5' end. Overview of removed sequences length count expect max.err error counts 19 1 0.0 1 1 20 1 0.0 2 1 21 1 0.0 2 1 22 1 0.0 2 1 23 5 0.0 2 5 25 32 0.0 2 32 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 35084 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 549.3 0 1 10 1 0.0 1 1 18 1 0.0 1 1 20 3 0.0 2 2 0 1 21 3 0.0 2 2 1 22 12 0.0 2 8 3 1 23 89 0.0 2 10 14 65 24 1123 0.0 2 177 921 25 25 33802 0.0 2 32686 1097 19 26 46 0.0 2 31 14 1 27 1 0.0 2 1 28 1 0.0 2 1 35 1 0.0 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodBP_186_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodBP_378_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodBP_186_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodBP_378_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 2.89 s (157 us/read; 0.38 M reads/minute). === Summary === Total read pairs processed: 18,471 Read 1 with adapter: 18,451 (99.9%) Read 2 with adapter: 18,461 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 18,471 (100.0%) Total basepairs processed: 9,222,453 bp Read 1: 4,611,463 bp Read 2: 4,610,990 bp Total written (filtered): 8,301,396 bp (90.0%) Read 1: 4,151,243 bp Read 2: 4,150,153 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 11 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 21 3 0.0 2 3 22 1 0.0 2 1 23 1 0.0 2 1 25 6 0.0 2 6 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 18440 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 19 3 0.0 1 2 1 20 2 0.0 2 1 1 21 3 0.0 2 1 2 22 9 0.0 2 0 1 8 23 90 0.0 2 6 20 64 24 822 0.0 2 116 683 23 25 17481 0.0 2 16722 741 18 26 29 0.0 2 16 12 1 27 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 10 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 10.0% C: 50.0% G: 20.0% T: 20.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 21 2 0.0 2 2 23 1 0.0 2 1 25 7 0.0 2 6 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 18451 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 20 1 0.0 2 1 21 3 0.0 2 1 1 1 22 6 0.0 2 2 2 2 23 41 0.0 2 3 7 31 24 582 0.0 2 82 484 16 25 17800 0.0 2 17203 584 13 26 16 0.0 2 4 11 1 27 1 0.0 2 0 1 28 1 0.0 2 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodK_187_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodK_379_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodK_187_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodK_379_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 3.23 s (175 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 18,459 Read 1 with adapter: 18,418 (99.8%) Read 2 with adapter: 18,431 (99.8%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 18,459 (100.0%) Total basepairs processed: 9,212,568 bp Read 1: 4,606,369 bp Read 2: 4,606,199 bp Total written (filtered): 8,293,111 bp (90.0%) Read 1: 4,147,024 bp Read 2: 4,146,087 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 17 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.5% C: 0.0% G: 17.6% T: 5.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 288.4 0 1 21 3 0.0 2 3 22 1 0.0 2 1 23 2 0.0 2 2 25 10 0.0 2 10 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 18401 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 288.4 0 1 19 2 0.0 1 2 20 4 0.0 2 2 2 21 5 0.0 2 4 1 22 9 0.0 2 7 2 23 82 0.0 2 6 20 56 24 833 0.0 2 125 672 36 25 17434 0.0 2 16256 1153 25 26 30 0.0 2 12 16 2 29 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 15 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 53.3% G: 0.0% T: 46.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 21 1 0.0 2 1 22 2 0.0 2 2 23 1 0.0 2 1 24 1 0.0 2 1 25 10 0.0 2 10 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 18416 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 288.4 0 1 19 1 0.0 1 0 1 21 1 0.0 2 1 22 3 0.0 2 2 0 1 23 38 0.0 2 3 3 32 24 557 0.0 2 82 463 12 25 17791 0.0 2 17177 602 12 26 24 0.0 2 14 10 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodL_188_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodL_380_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodL_188_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodL_380_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 2.93 s (179 us/read; 0.33 M reads/minute). === Summary === Total read pairs processed: 16,378 Read 1 with adapter: 16,344 (99.8%) Read 2 with adapter: 16,358 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 16,378 (100.0%) Total basepairs processed: 8,175,581 bp Read 1: 4,088,190 bp Read 2: 4,087,391 bp Total written (filtered): 7,359,471 bp (90.0%) Read 1: 3,680,428 bp Read 2: 3,679,043 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 6 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 83.3% C: 0.0% G: 0.0% T: 16.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 255.9 0 1 20 2 0.0 2 2 25 3 0.0 2 3 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 16338 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 20 1 0.0 2 0 1 21 2 0.0 2 2 22 4 0.0 2 2 1 1 23 55 0.0 2 6 7 42 24 697 0.0 2 96 551 50 25 15553 0.0 2 14267 1256 30 26 26 0.0 2 12 12 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 2 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 2 0.0 2 2 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 16356 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 20 1 0.0 2 1 21 1 0.0 2 1 22 2 0.0 2 1 1 23 46 0.0 2 5 7 34 24 515 0.0 2 74 429 12 25 15771 0.0 2 15213 544 14 26 20 0.0 2 12 8 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodLU_189_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodLU_381_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodLU_189_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodLU_381_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 6.44 s (164 us/read; 0.37 M reads/minute). === Summary === Total read pairs processed: 39,323 Read 1 with adapter: 39,231 (99.8%) Read 2 with adapter: 39,275 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 39,323 (100.0%) Total basepairs processed: 19,623,596 bp Read 1: 9,809,766 bp Read 2: 9,813,830 bp Total written (filtered): 17,664,680 bp (90.0%) Read 1: 8,831,231 bp Read 2: 8,833,449 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 26 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 73.1% C: 0.0% G: 23.1% T: 3.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 614.4 0 1 24 1 0.0 2 1 25 24 0.0 2 24 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 39205 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 11 1 0.0 1 0 1 14 1 0.0 1 0 1 17 1 0.0 1 1 18 1 0.0 1 1 20 4 0.0 2 1 2 1 21 9 0.0 2 7 0 2 22 16 0.0 2 4 2 10 23 159 0.0 2 13 37 109 24 1823 0.0 2 233 1480 110 25 37122 0.0 2 33886 3155 81 26 68 0.0 2 25 39 4 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 24 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 54.2% G: 12.5% T: 33.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 20 0.0 2 20 34 1 0.0 2 1 42 2 0.0 2 2 44 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 39251 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 19 2 0.0 1 2 20 3 0.0 2 2 1 21 6 0.0 2 3 1 2 22 12 0.0 2 4 2 6 23 112 0.0 2 12 17 83 24 1305 0.0 2 186 1089 30 25 37761 0.0 2 36461 1267 33 26 45 0.0 2 23 21 1 27 1 0.0 2 1 28 3 0.0 2 3 29 1 0.0 2 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodS_190_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodS_382_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodS_190_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodS_382_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 12.43 s (184 us/read; 0.33 M reads/minute). === Summary === Total read pairs processed: 67,533 Read 1 with adapter: 66,920 (99.1%) Read 2 with adapter: 66,973 (99.2%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 67,533 (100.0%) Total basepairs processed: 33,672,702 bp Read 1: 16,839,731 bp Read 2: 16,832,971 bp Total written (filtered): 30,331,557 bp (90.1%) Read 1: 15,170,481 bp Read 2: 15,161,076 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 265 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 67.5% C: 1.5% G: 30.6% T: 0.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 1055.2 0 5 4 1 263.8 0 1 20 7 0.0 2 7 24 6 0.0 2 4 1 1 25 245 0.0 2 240 5 26 1 0.0 2 0 1 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 66655 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 1 1055.2 0 1 4 1 263.8 0 1 14 1 0.0 1 1 15 1 0.0 1 0 1 18 1 0.0 1 0 1 19 2 0.0 1 2 20 12 0.0 2 4 6 2 21 17 0.0 2 8 3 6 22 21 0.0 2 6 6 9 23 257 0.0 2 26 44 187 24 2911 0.0 2 407 2319 185 25 63345 0.0 2 57609 5571 165 26 81 0.0 2 46 32 3 27 1 0.0 2 1 28 1 0.0 2 1 37 1 0.0 2 1 47 1 0.0 2 1 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 85 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.9% C: 68.2% G: 0.0% T: 5.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 25 72 0.0 2 43 24 5 32 1 0.0 2 1 34 11 0.0 2 11 35 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 66888 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 3 8 1055.2 0 8 4 1 263.8 0 1 5 1 66.0 0 1 14 1 0.0 1 1 16 2 0.0 1 1 1 19 2 0.0 1 0 2 20 5 0.0 2 4 1 21 12 0.0 2 8 4 22 17 0.0 2 6 5 6 23 146 0.0 2 16 24 106 24 1987 0.0 2 339 1593 55 25 64604 0.0 2 62399 2155 50 26 98 0.0 2 61 37 27 1 0.0 2 0 1 28 1 0.0 2 1 29 1 0.0 2 1 33 1 0.0 2 1 This is cutadapt 3.2 with Python 3.6.13 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodSU_191_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodSU_383_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodSU_191_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodSU_383_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... Finished in 4.62 s (178 us/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 25,970 Read 1 with adapter: 25,922 (99.8%) Read 2 with adapter: 25,938 (99.9%) Pairs that were too short: 0 (0.0%) Pairs written (passing filters): 25,970 (100.0%) Total basepairs processed: 12,958,871 bp Read 1: 6,475,662 bp Read 2: 6,483,209 bp Total written (filtered): 11,664,884 bp (90.0%) Read 1: 5,829,106 bp Read 2: 5,835,778 bp === First read: Adapter 1 === Sequence: GCATATCAATAAGCGGAGGA; Type: regular 3'; Length: 20; Trimmed: 23 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.3% C: 4.3% G: 17.4% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 20 1 0.0 2 1 21 2 0.0 2 2 22 1 0.0 2 1 23 1 0.0 2 1 24 2 0.0 2 2 25 16 0.0 2 16 === First read: Adapter 2 === Sequence: GCATCGATGAAGAACGCAGC; Type: regular 5'; Length: 20; Trimmed: 25899 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 14 2 0.0 1 2 15 1 0.0 1 1 17 1 0.0 1 0 1 20 3 0.0 2 1 2 21 5 0.0 2 2 1 2 22 4 0.0 2 1 0 3 23 125 0.0 2 15 25 85 24 1186 0.0 2 155 978 53 25 24523 0.0 2 22968 1515 40 26 49 0.0 2 23 24 2 === Second read: Adapter 3 === Sequence: GCTGCGTTCTTCATCGATGC; Type: regular 3'; Length: 20; Trimmed: 14 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.3% C: 71.4% G: 7.1% T: 7.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 22 2 0.0 2 2 23 1 0.0 2 1 24 1 0.0 2 1 25 9 0.0 2 7 2 32 1 0.0 2 1 === Second read: Adapter 4 === Sequence: TCCTCCGCTTATTGATATGC; Type: regular 5'; Length: 20; Trimmed: 25924 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Overview of removed sequences length count expect max.err error counts 18 1 0.0 1 1 20 2 0.0 2 1 1 21 4 0.0 2 2 1 1 22 5 0.0 2 1 0 4 23 56 0.0 2 5 11 40 24 883 0.0 2 137 727 19 25 24947 0.0 2 24129 798 20 26 26 0.0 2 15 11 Running external command line application. This may print messages to stdout and/or stderr. The commands to be run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist. Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C1_0_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C1_192_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C1_0_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C1_192_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C10_1_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C10_193_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C10_1_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C10_193_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C103_2_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C103_194_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C103_2_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C103_194_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C104_3_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C104_195_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C104_3_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C104_195_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C105_4_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C105_196_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C105_4_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C105_196_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C110_5_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C110_197_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C110_5_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C110_197_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C12_6_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C12_198_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C12_6_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C12_198_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C13_7_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C13_199_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C13_7_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C13_199_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C14_8_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C14_200_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C14_8_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C14_200_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C19_9_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C19_201_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C19_9_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C19_201_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C20_10_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C20_202_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C20_10_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C20_202_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C21_11_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C21_203_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C21_11_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C21_203_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C22_12_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C22_204_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C22_12_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C22_204_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C23_13_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C23_205_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C23_13_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C23_205_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C30_14_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C30_206_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C30_14_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C30_206_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C31_15_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C31_207_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C31_15_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C31_207_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C33_16_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C33_208_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C33_16_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C33_208_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C34_17_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C34_209_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C34_17_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C34_209_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C35_18_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C35_210_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C35_18_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C35_210_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C36_19_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C36_211_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C36_19_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C36_211_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C37_20_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C37_212_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C37_20_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C37_212_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C4_21_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C4_213_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C4_21_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C4_213_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C41_22_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C41_214_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C41_22_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C41_214_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C43_23_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C43_215_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C43_23_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C43_215_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C44_24_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C44_216_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C44_24_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C44_216_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C48_25_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C48_217_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C48_25_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C48_217_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C51_26_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C51_218_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C51_26_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C51_218_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C59_27_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C59_219_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C59_27_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C59_219_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C6_28_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C6_220_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C6_28_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C6_220_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C61_29_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C61_221_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C61_29_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C61_221_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C66_30_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C66_222_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C66_30_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C66_222_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C70_31_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C70_223_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C70_31_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C70_223_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C71_32_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C71_224_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C71_32_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C71_224_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C72_33_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C72_225_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C72_33_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C72_225_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C73_34_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C73_226_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C73_34_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C73_226_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C74_35_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C74_227_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C74_35_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C74_227_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C75_36_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C75_228_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C75_36_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C75_228_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C76_37_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C76_229_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C76_37_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C76_229_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C77_38_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C77_230_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C77_38_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C77_230_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C79_39_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C79_231_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C79_39_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C79_231_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C80_40_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C80_232_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C80_40_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C80_232_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C84_41_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C84_233_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C84_41_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C84_233_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C85_42_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C85_234_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C85_42_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C85_234_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C86_43_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C86_235_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C86_43_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C86_235_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C90_44_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C90_236_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C90_44_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C90_236_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C92_45_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C92_237_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C92_45_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C92_237_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C93_46_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C93_238_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C93_46_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C93_238_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C95_47_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C95_239_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C95_47_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C95_239_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C96_48_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C96_240_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C96_48_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C96_240_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C97_49_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/C97_241_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C97_49_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/C97_241_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep1_50_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep1_242_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep1_50_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep1_242_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep10_51_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep10_243_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep10_51_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep10_243_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep105_52_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep105_244_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep105_52_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep105_244_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep110_53_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep110_245_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep110_53_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep110_245_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep12_54_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep12_246_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep12_54_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep12_246_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep14_55_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep14_247_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep14_55_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep14_247_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep16_56_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep16_248_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep16_56_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep16_248_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep19_57_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep19_249_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep19_57_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep19_249_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep20_58_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep20_250_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep20_58_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep20_250_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep22_59_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep22_251_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep22_59_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep22_251_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep23_60_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep23_252_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep23_60_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep23_252_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep30_61_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep30_253_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep30_61_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep30_253_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep31_62_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep31_254_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep31_62_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep31_254_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep33_63_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep33_255_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep33_63_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep33_255_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep34_64_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep34_256_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep34_64_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep34_256_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep35_65_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep35_257_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep35_65_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep35_257_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep36_66_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep36_258_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep36_66_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep36_258_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep4_67_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep4_259_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep4_67_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep4_259_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep41_68_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep41_260_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep41_68_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep41_260_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep44_69_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep44_261_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep44_69_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep44_261_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep48_70_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep48_262_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep48_70_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep48_262_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep51_71_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep51_263_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep51_71_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep51_263_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep59_72_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep59_264_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep59_72_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep59_264_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep6_73_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep6_265_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep6_73_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep6_265_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep61_74_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep61_266_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep61_74_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep61_266_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep74_75_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep74_267_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep74_75_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep74_267_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep75_76_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep75_268_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep75_76_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep75_268_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep77_77_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep77_269_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep77_77_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep77_269_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep79_78_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep79_270_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep79_78_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep79_270_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep80_79_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep80_271_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep80_79_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep80_271_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep84_80_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep84_272_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep84_80_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep84_272_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep86_81_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep86_273_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep86_81_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep86_273_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep90_82_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep90_274_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep90_82_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep90_274_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep92_83_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep92_275_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep92_83_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep92_275_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep93_84_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep93_276_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep93_84_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep93_276_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep95_85_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep95_277_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep95_85_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep95_277_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep96_86_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep96_278_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep96_86_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep96_278_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep97_87_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hep97_279_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep97_87_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hep97_279_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm1_88_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm1_280_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm1_88_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm1_280_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm103_89_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm103_281_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm103_89_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm103_281_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm104_90_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm104_282_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm104_90_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm104_282_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm105_91_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm105_283_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm105_91_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm105_283_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm110_92_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm110_284_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm110_92_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm110_284_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm12_93_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm12_285_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm12_93_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm12_285_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm13_94_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm13_286_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm13_94_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm13_286_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm19_95_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm19_287_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm19_95_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm19_287_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm20_96_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm20_288_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm20_96_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm20_288_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm21_97_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm21_289_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm21_97_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm21_289_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm22_98_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm22_290_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm22_98_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm22_290_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm23_99_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm23_291_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm23_99_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm23_291_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm30_100_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm30_292_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm30_100_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm30_292_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm31_101_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm31_293_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm31_101_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm31_293_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm33_102_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm33_294_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm33_102_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm33_294_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm34_103_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm34_295_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm34_103_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm34_295_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm35_104_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm35_296_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm35_104_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm35_296_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm36_105_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm36_297_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm36_105_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm36_297_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm37_106_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm37_298_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm37_106_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm37_298_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm4_107_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm4_299_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm4_107_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm4_299_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm41_108_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm41_300_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm41_108_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm41_300_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm44_109_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm44_301_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm44_109_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm44_301_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm48_110_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm48_302_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm48_110_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm48_302_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm51_111_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm51_303_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm51_111_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm51_303_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm59_112_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm59_304_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm59_112_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm59_304_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm6_113_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm6_305_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm6_113_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm6_305_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm61_114_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm61_306_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm61_114_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm61_306_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm66_115_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm66_307_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm66_115_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm66_307_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm74_116_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm74_308_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm74_116_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm74_308_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm75_117_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm75_309_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm75_117_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm75_309_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm76_118_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm76_310_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm76_118_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm76_310_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm77_119_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm77_311_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm77_119_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm77_311_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm79_120_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm79_312_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm79_120_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm79_312_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm80_121_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm80_313_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm80_121_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm80_313_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm84_122_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm84_314_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm84_122_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm84_314_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm85_123_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm85_315_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm85_123_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm85_315_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm86_124_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm86_316_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm86_124_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm86_316_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm90_125_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm90_317_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm90_125_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm90_317_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm92_126_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm92_318_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm92_126_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm92_318_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm95_127_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm95_319_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm95_127_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm95_319_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm96_128_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/Hm96_320_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm96_128_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/Hm96_320_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O10_129_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O10_321_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O10_129_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O10_321_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O103_130_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O103_322_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O103_130_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O103_322_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O105_131_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O105_323_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O105_131_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O105_323_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O12_132_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O12_324_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O12_132_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O12_324_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O13_133_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O13_325_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O13_133_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O13_325_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O14_134_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O14_326_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O14_134_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O14_326_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O16_135_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O16_327_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O16_135_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O16_327_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O19_136_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O19_328_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O19_136_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O19_328_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O20_137_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O20_329_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O20_137_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O20_329_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O21_138_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O21_330_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O21_138_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O21_330_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O22_139_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O22_331_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O22_139_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O22_331_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O23_140_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O23_332_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O23_140_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O23_332_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O30_141_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O30_333_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O30_141_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O30_333_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O34_142_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O34_334_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O34_142_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O34_334_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O35_143_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O35_335_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O35_143_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O35_335_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O36_144_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O36_336_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O36_144_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O36_336_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O37_145_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O37_337_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O37_145_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O37_337_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O41_146_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O41_338_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O41_146_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O41_338_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O43_147_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O43_339_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O43_147_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O43_339_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O48_148_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O48_340_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O48_148_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O48_340_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O51_149_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O51_341_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O51_149_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O51_341_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O6_150_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O6_342_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O6_150_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O6_342_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O61_151_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O61_343_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O61_151_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O61_343_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O66_152_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O66_344_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O66_152_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O66_344_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O70_153_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O70_345_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O70_153_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O70_345_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O71_154_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O71_346_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O71_154_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O71_346_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O72_155_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O72_347_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O72_155_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O72_347_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O73_156_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O73_348_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O73_156_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O73_348_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O74_157_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O74_349_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O74_157_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O74_349_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O75_158_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O75_350_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O75_158_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O75_350_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O76_159_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O76_351_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O76_159_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O76_351_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O77_160_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O77_352_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O77_160_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O77_352_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O80_161_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O80_353_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O80_161_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O80_353_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O84_162_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O84_354_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O84_162_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O84_354_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O85_163_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O85_355_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O85_163_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O85_355_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O86_164_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O86_356_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O86_164_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O86_356_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O90_165_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O90_357_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O90_165_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O90_357_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O93_166_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O93_358_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O93_166_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O93_358_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O97_167_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/O97_359_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O97_167_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/O97_359_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedBP1_168_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedBP1_360_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedBP1_168_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedBP1_360_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedBP3_170_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedBP3_362_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedBP3_170_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedBP3_362_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedBp2_169_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedBp2_361_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedBp2_169_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedBp2_361_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedK1_171_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedK1_363_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedK1_171_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedK1_363_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedK2_172_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedK2_364_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedK2_172_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedK2_364_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedK3_173_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedK3_365_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedK3_173_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedK3_365_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedL1_174_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedL1_366_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedL1_174_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedL1_366_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedL2_175_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedL2_367_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedL2_175_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedL2_367_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedL3_176_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedL3_368_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedL3_176_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedL3_368_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedLU1_177_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedLU1_369_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedLU1_177_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedLU1_369_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedLU2_178_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedLU2_370_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedLU2_178_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedLU2_370_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedLU3_179_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedLU3_371_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedLU3_179_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedLU3_371_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedS1_180_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedS1_372_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedS1_180_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedS1_372_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedS2_181_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedS2_373_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedS2_181_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedS2_373_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedS3_182_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedS3_374_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedS3_182_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedS3_374_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedSU1_183_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedSU1_375_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedSU1_183_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedSU1_375_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedSU2_184_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedSU2_376_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedSU2_184_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedSU2_376_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedSU3_185_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/sedSU3_377_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedSU3_185_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/sedSU3_377_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodBP_186_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodBP_378_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodBP_186_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodBP_378_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodK_187_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodK_379_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodK_187_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodK_379_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodL_188_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodL_380_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodL_188_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodL_380_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodLU_189_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodLU_381_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodLU_189_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodLU_381_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodS_190_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodS_382_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodS_190_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodS_382_L001_R2_001.fastq.gz Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodSU_191_L001_R1_001.fastq.gz -p /scratch/1232918.1.a16.q/q2-CasavaOneEightSingleLanePerSampleDirFmt-1wm2g8c_/vodSU_383_L001_R2_001.fastq.gz --adapter GCATATCAATAAGCGGAGGA --front GCATCGATGAAGAACGCAGC -A GCTGCGTTCTTCATCGATGC -G TCCTCCGCTTATTGATATGC /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodSU_191_L001_R1_001.fastq.gz /scratch/1232918.1.a16.q/qiime2-archive-sh8n0pdr/6499c3b8-e92c-4aab-bd9d-d71bea6db184/data/vodSU_383_L001_R2_001.fastq.gz Saved SampleData[PairedEndSequencesWithQuality] to: demux-trimmed.qza Saved Visualization to: demux-trimmed.qzv