Running external command line application. This may print messages to stdout and/or stderr. The commands to be run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist. Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM1_0_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM1_16_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM1_0_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM1_16_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM1_0_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM1_16_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM1_0_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM1_16_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8<----------] 00:00:01 39,482 reads @ 28.1 µs/read; 2.13 M reads/minute Finished in 1.13 s (29 µs/read; 2.10 M reads/minute). === Summary === Total read pairs processed: 39,482 Read 1 with adapter: 36,870 (93.4%) Read 2 with adapter: 37,970 (96.2%) Pairs that were too short: 38,181 (96.7%) Pairs written (passing filters): 1,301 (3.3%) Total basepairs processed: 23,768,164 bp Read 1: 11,884,082 bp Read 2: 11,884,082 bp Total written (filtered): 778,719 bp (3.3%) Read 1: 387,294 bp Read 2: 391,425 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 36870 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.1% G: 0.1% T: 0.0% none/other: 99.7% Overview of removed sequences length count expect max.err error counts 3 19 616.9 0 19 4 7 154.2 0 7 5 4 38.6 0 4 10 2 0.0 1 0 2 127 14 0.0 1 12 2 131 2 0.0 1 2 132 6 0.0 1 6 133 1 0.0 1 1 149 2 0.0 1 1 1 151 3 0.0 1 3 152 6 0.0 1 5 1 293 1 0.0 1 1 299 10 0.0 1 0 10 300 33 0.0 1 20 13 301 36760 0.0 1 30455 6305 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 37970 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.1% G: 0.0% T: 0.1% none/other: 99.8% Overview of removed sequences length count expect max.err error counts 3 4 616.9 0 4 4 6 154.2 0 6 5 11 38.6 0 11 6 1 9.6 0 1 9 1 0.2 0 1 10 3 0.0 1 2 1 11 3 0.0 1 1 2 12 2 0.0 1 1 1 15 1 0.0 1 0 1 299 1 0.0 2 0 0 1 300 40 0.0 2 29 11 301 37897 0.0 2 35964 1756 177 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM10_9_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM10_25_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM10_9_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM10_25_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM10_9_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM10_25_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM10_9_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM10_25_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8=----------] 00:00:01 47,664 reads @ 27.8 µs/read; 2.16 M reads/minute Finished in 1.34 s (28 µs/read; 2.13 M reads/minute). === Summary === Total read pairs processed: 47,664 Read 1 with adapter: 45,147 (94.7%) Read 2 with adapter: 46,648 (97.9%) Pairs that were too short: 46,751 (98.1%) Pairs written (passing filters): 913 (1.9%) Total basepairs processed: 28,693,728 bp Read 1: 14,346,864 bp Read 2: 14,346,864 bp Total written (filtered): 545,325 bp (1.9%) Read 1: 271,823 bp Read 2: 273,502 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 45147 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.1% G: 0.1% T: 0.0% none/other: 99.8% Overview of removed sequences length count expect max.err error counts 3 15 744.8 0 15 4 9 186.2 0 9 5 1 46.5 0 1 6 1 11.6 0 1 126 1 0.0 1 1 127 9 0.0 1 6 3 131 2 0.0 1 2 132 7 0.0 1 6 1 133 1 0.0 1 1 149 2 0.0 1 1 1 151 1 0.0 1 1 152 7 0.0 1 5 2 264 1 0.0 1 1 282 1 0.0 1 1 299 17 0.0 1 0 17 300 37 0.0 1 28 9 301 45035 0.0 1 37567 7468 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 46648 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.1% G: 0.0% T: 0.0% none/other: 99.8% Overview of removed sequences length count expect max.err error counts 3 3 744.8 0 3 4 16 186.2 0 16 5 3 46.5 0 3 6 2 11.6 0 2 11 1 0.0 1 0 1 13 1 0.0 1 1 14 1 0.0 1 0 1 269 2 0.0 2 0 0 2 270 1 0.0 2 0 1 300 45 0.0 2 33 11 1 301 46573 0.0 2 44285 2103 185 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM11_10_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM11_26_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM11_10_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM11_26_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM11_10_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM11_26_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM11_10_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM11_26_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8<----------] 00:00:00 2,364 reads @ 32.1 µs/read; 1.87 M reads/minute Finished in 0.08 s (36 µs/read; 1.68 M reads/minute). === Summary === Total read pairs processed: 2,364 Read 1 with adapter: 2,172 (91.9%) Read 2 with adapter: 2,253 (95.3%) Pairs that were too short: 2,264 (95.8%) Pairs written (passing filters): 100 (4.2%) Total basepairs processed: 1,423,128 bp Read 1: 711,564 bp Read 2: 711,564 bp Total written (filtered): 59,095 bp (4.2%) Read 1: 29,004 bp Read 2: 30,091 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 2172 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.4% G: 0.0% T: 0.1% none/other: 99.4% Overview of removed sequences length count expect max.err error counts 3 2 36.9 0 2 4 1 9.2 0 1 127 4 0.0 1 4 132 1 0.0 1 1 149 1 0.0 1 0 1 152 2 0.0 1 2 299 2 0.0 1 0 2 301 2159 0.0 1 1788 371 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 2253 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 0.0% T: 0.0% none/other: 99.9% Overview of removed sequences length count expect max.err error counts 4 1 9.2 0 1 5 1 2.3 0 1 301 2251 0.0 2 2045 189 17 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM2_1_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM2_17_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM2_1_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM2_17_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM2_1_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM2_17_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM2_1_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM2_17_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8<----------] 00:00:00 35,446 reads @ 27.7 µs/read; 2.16 M reads/minute Finished in 1.00 s (28 µs/read; 2.13 M reads/minute). === Summary === Total read pairs processed: 35,446 Read 1 with adapter: 33,137 (93.5%) Read 2 with adapter: 34,363 (96.9%) Pairs that were too short: 34,403 (97.1%) Pairs written (passing filters): 1,043 (2.9%) Total basepairs processed: 21,338,492 bp Read 1: 10,669,246 bp Read 2: 10,669,246 bp Total written (filtered): 624,594 bp (2.9%) Read 1: 310,763 bp Read 2: 313,831 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 33137 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.1% G: 0.1% T: 0.0% none/other: 99.7% Overview of removed sequences length count expect max.err error counts 3 19 553.8 0 19 4 6 138.5 0 6 5 2 34.6 0 2 6 2 8.7 0 2 10 1 0.0 1 0 1 127 10 0.0 1 9 1 128 1 0.0 1 1 131 1 0.0 1 1 132 6 0.0 1 6 133 1 0.0 1 1 152 5 0.0 1 3 2 282 1 0.0 1 1 299 6 0.0 1 0 6 300 26 0.0 1 19 7 301 33050 0.0 1 27207 5843 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 34363 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.1% G: 0.0% T: 0.0% none/other: 99.9% Overview of removed sequences length count expect max.err error counts 3 2 553.8 0 2 4 9 138.5 0 9 5 3 34.6 0 3 6 1 8.7 0 1 8 2 0.5 0 2 9 3 0.1 0 2 1 10 1 0.0 1 1 11 1 0.0 1 1 300 24 0.0 2 14 10 301 34317 0.0 2 32647 1501 169 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM3_2_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM3_18_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM3_2_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM3_18_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM3_2_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM3_18_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM3_2_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM3_18_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8<----------] 00:00:00 33,674 reads @ 27.9 µs/read; 2.15 M reads/minute Finished in 0.96 s (28 µs/read; 2.11 M reads/minute). === Summary === Total read pairs processed: 33,674 Read 1 with adapter: 31,672 (94.1%) Read 2 with adapter: 32,704 (97.1%) Pairs that were too short: 32,782 (97.4%) Pairs written (passing filters): 892 (2.6%) Total basepairs processed: 20,271,748 bp Read 1: 10,135,874 bp Read 2: 10,135,874 bp Total written (filtered): 532,569 bp (2.6%) Read 1: 264,739 bp Read 2: 267,830 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 31672 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.1% G: 0.1% T: 0.0% none/other: 99.8% Overview of removed sequences length count expect max.err error counts 3 10 526.2 0 10 4 5 131.5 0 5 127 8 0.0 1 8 131 1 0.0 1 1 132 8 0.0 1 8 133 1 0.0 1 1 134 1 0.0 1 0 1 149 2 0.0 1 2 152 6 0.0 1 4 2 299 6 0.0 1 0 6 300 23 0.0 1 14 9 301 31601 0.0 1 26127 5474 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 32704 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.1% G: 0.0% T: 0.0% none/other: 99.9% Overview of removed sequences length count expect max.err error counts 3 2 526.2 0 2 4 11 131.5 0 11 5 4 32.9 0 4 13 1 0.0 1 1 300 28 0.0 2 18 10 301 32658 0.0 2 31131 1397 130 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM4_3_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM4_19_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM4_3_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM4_19_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM4_3_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM4_19_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM4_3_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM4_19_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8=----------] 00:00:01 41,128 reads @ 28.0 µs/read; 2.15 M reads/minute Finished in 1.17 s (28 µs/read; 2.11 M reads/minute). === Summary === Total read pairs processed: 41,128 Read 1 with adapter: 38,374 (93.3%) Read 2 with adapter: 39,787 (96.7%) Pairs that were too short: 39,880 (97.0%) Pairs written (passing filters): 1,248 (3.0%) Total basepairs processed: 24,759,056 bp Read 1: 12,379,528 bp Read 2: 12,379,528 bp Total written (filtered): 747,561 bp (3.0%) Read 1: 372,675 bp Read 2: 374,886 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 38374 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.1% G: 0.1% T: 0.0% none/other: 99.7% Overview of removed sequences length count expect max.err error counts 3 26 642.6 0 26 4 3 160.7 0 3 8 1 0.6 0 1 9 1 0.2 0 0 1 10 2 0.0 1 0 2 126 3 0.0 1 3 127 9 0.0 1 9 131 1 0.0 1 0 1 132 6 0.0 1 6 133 1 0.0 1 0 1 149 1 0.0 1 1 150 1 0.0 1 1 151 2 0.0 1 2 152 4 0.0 1 2 2 278 2 0.0 1 2 282 2 0.0 1 2 299 13 0.0 1 0 13 300 36 0.0 1 26 10 301 38260 0.0 1 31443 6817 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 39787 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.1% G: 0.0% T: 0.1% none/other: 99.8% Overview of removed sequences length count expect max.err error counts 3 8 642.6 0 8 4 13 160.7 0 13 5 9 40.2 0 9 7 3 2.5 0 3 8 1 0.6 0 1 10 1 0.0 1 1 13 1 0.0 1 1 17 1 0.0 1 0 1 300 38 0.0 2 28 10 301 39712 0.0 2 37449 2064 199 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM5_4_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM5_20_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM5_4_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM5_20_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM5_4_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM5_20_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM5_4_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM5_20_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8=----------] 00:00:01 46,485 reads @ 28.2 µs/read; 2.13 M reads/minute Finished in 1.33 s (29 µs/read; 2.10 M reads/minute). === Summary === Total read pairs processed: 46,485 Read 1 with adapter: 43,124 (92.8%) Read 2 with adapter: 44,797 (96.4%) Pairs that were too short: 44,820 (96.4%) Pairs written (passing filters): 1,665 (3.6%) Total basepairs processed: 27,983,970 bp Read 1: 13,991,985 bp Read 2: 13,991,985 bp Total written (filtered): 997,068 bp (3.6%) Read 1: 497,267 bp Read 2: 499,801 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 43124 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.1% G: 0.1% T: 0.1% none/other: 99.7% Overview of removed sequences length count expect max.err error counts 3 64 726.3 0 64 4 13 181.6 0 13 5 3 45.4 0 3 6 1 11.3 0 1 126 2 0.0 1 1 1 127 12 0.0 1 8 4 131 2 0.0 1 1 1 132 5 0.0 1 4 1 133 1 0.0 1 1 150 1 0.0 1 1 151 1 0.0 1 1 152 7 0.0 1 7 299 11 0.0 1 0 11 300 23 0.0 1 16 7 301 42978 0.0 1 35349 7629 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 44797 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.1% G: 0.0% T: 0.3% none/other: 99.6% Overview of removed sequences length count expect max.err error counts 3 4 726.3 0 4 4 18 181.6 0 18 5 19 45.4 0 19 6 1 11.3 0 1 7 6 2.8 0 6 8 17 0.7 0 17 9 10 0.2 0 10 10 16 0.0 1 13 3 11 22 0.0 1 12 10 12 9 0.0 1 7 2 13 6 0.0 1 6 14 1 0.0 1 0 1 15 3 0.0 1 0 3 300 28 0.0 2 23 5 301 44637 0.0 2 42318 2117 202 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM6_5_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM6_21_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM6_5_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM6_21_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM6_5_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM6_21_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM6_5_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM6_21_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8=----------] 00:00:01 48,408 reads @ 27.8 µs/read; 2.16 M reads/minute Finished in 1.36 s (28 µs/read; 2.13 M reads/minute). === Summary === Total read pairs processed: 48,408 Read 1 with adapter: 45,376 (93.7%) Read 2 with adapter: 46,973 (97.0%) Pairs that were too short: 47,112 (97.3%) Pairs written (passing filters): 1,296 (2.7%) Total basepairs processed: 29,141,616 bp Read 1: 14,570,808 bp Read 2: 14,570,808 bp Total written (filtered): 775,542 bp (2.7%) Read 1: 386,174 bp Read 2: 389,368 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 45376 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.1% G: 0.0% T: 0.0% none/other: 99.7% Overview of removed sequences length count expect max.err error counts 3 28 756.4 0 28 4 4 189.1 0 4 5 3 47.3 0 3 10 1 0.0 1 0 1 126 3 0.0 1 3 127 14 0.0 1 13 1 131 1 0.0 1 1 132 9 0.0 1 8 1 151 2 0.0 1 2 152 3 0.0 1 1 2 282 1 0.0 1 1 299 11 0.0 1 0 11 300 35 0.0 1 27 8 301 45261 0.0 1 37444 7817 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 46973 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.1% G: 0.0% T: 0.0% none/other: 99.9% Overview of removed sequences length count expect max.err error counts 3 8 756.4 0 8 4 18 189.1 0 18 5 5 47.3 0 5 6 1 11.8 0 1 8 1 0.7 0 1 10 1 0.0 1 1 13 1 0.0 1 0 1 300 31 0.0 2 20 10 1 301 46907 0.0 2 44766 1943 198 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM7_6_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM7_22_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM7_6_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM7_22_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM7_6_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM7_22_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM7_6_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM7_22_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8=----------] 00:00:01 40,817 reads @ 28.7 µs/read; 2.09 M reads/minute Finished in 1.19 s (29 µs/read; 2.06 M reads/minute). === Summary === Total read pairs processed: 40,817 Read 1 with adapter: 35,829 (87.8%) Read 2 with adapter: 36,939 (90.5%) Pairs that were too short: 37,025 (90.7%) Pairs written (passing filters): 3,792 (9.3%) Total basepairs processed: 24,571,834 bp Read 1: 12,285,917 bp Read 2: 12,285,917 bp Total written (filtered): 2,276,797 bp (9.3%) Read 1: 1,136,206 bp Read 2: 1,140,591 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 35829 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.2% C: 0.1% G: 0.1% T: 0.1% none/other: 99.5% Overview of removed sequences length count expect max.err error counts 3 78 637.8 0 78 4 29 159.4 0 29 5 6 39.9 0 6 6 6 10.0 0 6 127 12 0.0 1 10 2 131 5 0.0 1 5 132 10 0.0 1 6 4 149 3 0.0 1 2 1 151 1 0.0 1 1 152 7 0.0 1 4 3 282 1 0.0 1 1 299 12 0.0 1 0 12 300 26 0.0 1 17 9 301 35633 0.0 1 29485 6148 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 36939 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.1% G: 0.0% T: 0.1% none/other: 99.7% Overview of removed sequences length count expect max.err error counts 3 10 637.8 0 10 4 23 159.4 0 23 5 13 39.9 0 13 7 4 2.5 0 4 8 9 0.6 0 9 10 3 0.0 1 1 2 11 7 0.0 1 4 3 12 5 0.0 1 1 4 13 3 0.0 1 3 14 1 0.0 1 1 23 1 0.0 2 1 269 1 0.0 2 0 0 1 300 29 0.0 2 18 11 301 36830 0.0 2 34871 1798 161 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM8_7_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM8_23_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM8_7_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM8_23_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM8_7_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM8_23_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM8_7_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM8_23_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8=----------] 00:00:01 43,688 reads @ 28.2 µs/read; 2.13 M reads/minute Finished in 1.25 s (29 µs/read; 2.10 M reads/minute). === Summary === Total read pairs processed: 43,688 Read 1 with adapter: 40,938 (93.7%) Read 2 with adapter: 42,330 (96.9%) Pairs that were too short: 42,415 (97.1%) Pairs written (passing filters): 1,273 (2.9%) Total basepairs processed: 26,300,176 bp Read 1: 13,150,088 bp Read 2: 13,150,088 bp Total written (filtered): 762,090 bp (2.9%) Read 1: 379,356 bp Read 2: 382,734 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 40938 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.1% G: 0.1% T: 0.0% none/other: 99.7% Overview of removed sequences length count expect max.err error counts 3 24 682.6 0 24 4 6 170.7 0 6 9 1 0.2 0 0 1 126 2 0.0 1 1 1 127 10 0.0 1 9 1 131 1 0.0 1 1 132 15 0.0 1 15 146 1 0.0 1 1 151 1 0.0 1 1 152 4 0.0 1 4 282 1 0.0 1 1 283 1 0.0 1 1 299 17 0.0 1 0 17 300 41 0.0 1 34 7 301 40813 0.0 1 33828 6985 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 42330 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.1% G: 0.0% T: 0.0% none/other: 99.9% Overview of removed sequences length count expect max.err error counts 3 8 682.6 0 8 4 13 170.7 0 13 5 9 42.7 0 9 9 1 0.2 0 1 10 4 0.0 1 2 2 12 1 0.0 1 0 1 300 25 0.0 2 13 11 1 301 42269 0.0 2 39483 2594 192 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM9_8_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM9_24_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM9_8_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM9_24_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM9_8_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/GM9_24_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM9_8_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/GM9_24_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8<----------] 00:00:01 34,823 reads @ 29.0 µs/read; 2.07 M reads/minute Finished in 1.03 s (30 µs/read; 2.03 M reads/minute). === Summary === Total read pairs processed: 34,823 Read 1 with adapter: 32,820 (94.2%) Read 2 with adapter: 33,935 (97.4%) Pairs that were too short: 34,060 (97.8%) Pairs written (passing filters): 763 (2.2%) Total basepairs processed: 20,963,446 bp Read 1: 10,481,723 bp Read 2: 10,481,723 bp Total written (filtered): 457,521 bp (2.2%) Read 1: 227,931 bp Read 2: 229,590 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 32820 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.1% G: 0.0% T: 0.0% none/other: 99.8% Overview of removed sequences length count expect max.err error counts 3 12 544.1 0 12 4 9 136.0 0 9 6 1 8.5 0 1 9 2 0.1 0 1 1 126 1 0.0 1 1 127 4 0.0 1 3 1 131 1 0.0 1 1 132 5 0.0 1 5 151 1 0.0 1 1 152 4 0.0 1 4 153 1 0.0 1 1 282 1 0.0 1 1 299 8 0.0 1 0 8 300 27 0.0 1 18 9 301 32743 0.0 1 26153 6590 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 33935 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 0.0% T: 0.0% none/other: 99.9% Overview of removed sequences length count expect max.err error counts 3 1 544.1 0 1 4 6 136.0 0 6 5 6 34.0 0 6 7 1 2.1 0 1 10 1 0.0 1 1 12 1 0.0 1 0 1 268 1 0.0 2 0 0 1 300 17 0.0 2 10 7 301 33901 0.0 2 30777 2921 203 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/NC1_14_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/NC1_30_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/NC1_14_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/NC1_30_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/NC1_14_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/NC1_30_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/NC1_14_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/NC1_30_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8<----------] 00:00:00 78 reads @ 144.1 µs/read; 0.42 M reads/minute Finished in 0.02 s (228 µs/read; 0.26 M reads/minute). === Summary === Total read pairs processed: 78 Read 1 with adapter: 4 (5.1%) Read 2 with adapter: 4 (5.1%) Pairs that were too short: 2 (2.6%) Pairs written (passing filters): 76 (97.4%) Total basepairs processed: 46,956 bp Read 1: 23,478 bp Read 2: 23,478 bp Total written (filtered): 45,723 bp (97.4%) Read 1: 22,869 bp Read 2: 22,854 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 4 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 25.0% T: 25.0% none/other: 50.0% Overview of removed sequences length count expect max.err error counts 3 1 1.2 0 1 4 1 0.3 0 1 301 2 0.0 1 2 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 4 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 25.0% G: 0.0% T: 0.0% none/other: 25.0% Overview of removed sequences length count expect max.err error counts 6 2 0.0 0 2 10 1 0.0 1 0 1 301 1 0.0 2 1 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/NC2_15_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/NC2_31_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/NC2_15_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/NC2_31_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/NC2_15_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/NC2_31_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/NC2_15_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/NC2_31_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8<----------] 00:00:00 205 reads @ 74.6 µs/read; 0.80 M reads/minute Finished in 0.02 s (107 µs/read; 0.56 M reads/minute). === Summary === Total read pairs processed: 205 Read 1 with adapter: 141 (68.8%) Read 2 with adapter: 121 (59.0%) Pairs that were too short: 121 (59.0%) Pairs written (passing filters): 84 (41.0%) Total basepairs processed: 123,410 bp Read 1: 61,705 bp Read 2: 61,705 bp Total written (filtered): 47,410 bp (38.4%) Read 1: 22,126 bp Read 2: 25,284 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 141 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.7% C: 13.5% G: 0.0% T: 2.8% none/other: 83.0% Overview of removed sequences length count expect max.err error counts 3 1 3.2 0 1 127 7 0.0 1 6 1 131 2 0.0 1 2 132 4 0.0 1 4 133 2 0.0 1 2 151 6 0.0 1 5 1 152 2 0.0 1 2 301 117 0.0 1 104 13 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 121 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 0.0% T: 0.0% none/other: 100.0% Overview of removed sequences length count expect max.err error counts 301 121 0.0 2 116 5 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/PC1_11_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/PC1_27_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/PC1_11_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/PC1_27_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/PC1_11_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/PC1_27_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/PC1_11_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/PC1_27_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8=----------] 00:00:01 42,109 reads @ 26.7 µs/read; 2.25 M reads/minute Finished in 1.14 s (27 µs/read; 2.22 M reads/minute). === Summary === Total read pairs processed: 42,109 Read 1 with adapter: 40,303 (95.7%) Read 2 with adapter: 41,208 (97.9%) Pairs that were too short: 41,291 (98.1%) Pairs written (passing filters): 818 (1.9%) Total basepairs processed: 25,349,618 bp Read 1: 12,674,809 bp Read 2: 12,674,809 bp Total written (filtered): 490,476 bp (1.9%) Read 1: 244,366 bp Read 2: 246,110 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 40303 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.1% G: 0.0% T: 0.0% none/other: 99.8% Overview of removed sequences length count expect max.err error counts 3 17 658.0 0 17 4 6 164.5 0 6 5 2 41.1 0 2 6 1 10.3 0 1 9 1 0.2 0 1 127 4 0.0 1 3 1 131 2 0.0 1 1 1 132 5 0.0 1 4 1 149 2 0.0 1 2 152 4 0.0 1 3 1 283 1 0.0 1 1 299 16 0.0 1 0 16 300 34 0.0 1 26 8 301 40208 0.0 1 35076 5132 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 41208 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 0.0% T: 0.0% none/other: 99.9% Overview of removed sequences length count expect max.err error counts 3 4 658.0 0 4 4 11 164.5 0 11 5 4 41.1 0 4 8 1 0.6 0 1 9 1 0.2 0 0 1 10 1 0.0 1 0 1 12 1 0.0 1 0 1 271 1 0.0 2 0 0 1 300 21 0.0 2 14 7 301 41163 0.0 2 39377 1639 147 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/PC2_12_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/PC2_28_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/PC2_12_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/PC2_28_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/PC2_12_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/PC2_28_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/PC2_12_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/PC2_28_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8<----------] 00:00:01 37,673 reads @ 27.3 µs/read; 2.20 M reads/minute Finished in 1.05 s (28 µs/read; 2.16 M reads/minute). === Summary === Total read pairs processed: 37,673 Read 1 with adapter: 35,923 (95.4%) Read 2 with adapter: 36,637 (97.3%) Pairs that were too short: 36,749 (97.5%) Pairs written (passing filters): 924 (2.5%) Total basepairs processed: 22,679,146 bp Read 1: 11,339,573 bp Read 2: 11,339,573 bp Total written (filtered): 553,373 bp (2.4%) Read 1: 275,726 bp Read 2: 277,647 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 35923 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.1% G: 0.0% T: 0.1% none/other: 99.7% Overview of removed sequences length count expect max.err error counts 3 25 588.6 0 25 4 4 147.2 0 4 5 4 36.8 0 4 11 1 0.0 1 0 1 127 6 0.0 1 6 132 8 0.0 1 8 150 1 0.0 1 1 152 1 0.0 1 1 266 1 0.0 1 1 282 1 0.0 1 1 299 16 0.0 1 0 16 300 27 0.0 1 21 6 301 35828 0.0 1 31271 4557 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 36637 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.1% G: 0.0% T: 0.0% none/other: 99.9% Overview of removed sequences length count expect max.err error counts 3 4 588.6 0 4 4 13 147.2 0 13 5 8 36.8 0 8 6 1 9.2 0 1 8 1 0.6 0 1 9 1 0.1 0 1 10 3 0.0 1 2 1 11 1 0.0 1 0 1 12 1 0.0 1 1 269 1 0.0 2 0 0 1 300 18 0.0 2 13 5 301 36585 0.0 2 34107 2316 162 Command: cutadapt --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/PC3_13_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/PC3_29_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/PC3_13_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/PC3_29_L001_R2_001.fastq.gz This is cutadapt 3.4 with Python 3.8.8 Command line parameters: --cores 1 --error-rate 0.1 --times 1 --overlap 3 --minimum-length 1 -o /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/PC3_13_L001_R1_001.fastq.gz -p /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-_xoa_2yh/PC3_29_L001_R2_001.fastq.gz --adapter GTGYCAGCMGCCGCGGTAA -A CCGYCAATTYMTTTRAGTTT /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/PC3_13_L001_R1_001.fastq.gz /var/folders/d7/0rpznrmn0rvfhz7qb56q0f2h0000gn/T/qiime2-archive-3meqdqz2/86da2cbf-fdcc-4a25-ae47-00378c8e08b3/data/PC3_29_L001_R2_001.fastq.gz Processing reads on 1 core in paired-end mode ... [8=----------] 00:00:01 41,863 reads @ 28.3 µs/read; 2.12 M reads/minute Finished in 1.21 s (29 µs/read; 2.08 M reads/minute). === Summary === Total read pairs processed: 41,863 Read 1 with adapter: 39,783 (95.0%) Read 2 with adapter: 40,782 (97.4%) Pairs that were too short: 40,897 (97.7%) Pairs written (passing filters): 966 (2.3%) Total basepairs processed: 25,201,526 bp Read 1: 12,600,763 bp Read 2: 12,600,763 bp Total written (filtered): 577,752 bp (2.3%) Read 1: 287,437 bp Read 2: 290,315 bp === First read: Adapter 1 === Sequence: GTGYCAGCMGCCGCGGTAA; Type: regular 3'; Length: 19; Trimmed: 39783 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.1% G: 0.1% T: 0.1% none/other: 99.6% Overview of removed sequences length count expect max.err error counts 3 30 654.1 0 30 4 16 163.5 0 16 5 1 40.9 0 1 6 2 10.2 0 2 9 2 0.2 0 0 2 10 1 0.0 1 0 1 126 3 0.0 1 3 127 17 0.0 1 15 2 131 3 0.0 1 3 132 10 0.0 1 10 149 1 0.0 1 1 151 5 0.0 1 4 1 152 8 0.0 1 7 1 282 2 0.0 1 2 299 17 0.0 1 0 17 300 29 0.0 1 23 6 301 39636 0.0 1 33539 6097 === Second read: Adapter 2 === Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 3'; Length: 20; Trimmed: 40782 times No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.1% G: 0.0% T: 0.1% none/other: 99.8% Overview of removed sequences length count expect max.err error counts 3 11 654.1 0 11 4 9 163.5 0 9 5 2 40.9 0 2 7 4 2.6 0 4 8 1 0.6 0 1 10 1 0.0 1 1 12 3 0.0 1 1 2 14 1 0.0 1 0 1 270 1 0.0 2 0 0 1 300 30 0.0 2 23 7 301 40719 0.0 2 38365 2153 201