Running external command line application(s). This may print messages to stdout and/or stderr.
The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist.

Command: run_aldex2.R /tmp/tmp13arye_q/input.tsv.biom /tmp/tmp13arye_q/input.map.txt UV 128 glm all /tmp/tmp13arye_q/output.summary.txt

R version 4.0.2 (2020-06-22) 
Lade nötiges Paket: zCompositions
Lade nötiges Paket: MASS
Lade nötiges Paket: NADA
Lade nötiges Paket: survival

Attache Paket: ‘NADA’

The following object is masked from ‘package:stats’:

    cor

Lade nötiges Paket: truncnorm
aldex.clr: generating Monte-Carlo instances and clr values
operating in serial mode
computing center with all features
aldex.glm: doing glm test based on a model matrix
Fehler in lr2glm(mci_lr, conditions, ...) : 
  Please define the aldex.clr object for a model.matrix 'conditions'.
Ruft auf: aldex -> aldex.glm -> lr2glm
Ausführung angehalten
Traceback (most recent call last):
  File "/home/bt140047/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/q2_aldex2/_method.py", line 59, in aldex2
    run_commands([cmd])
  File "/home/bt140047/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/q2_aldex2/_method.py", line 21, in run_commands
    subprocess.run(cmd, check=True)
  File "/home/bt140047/miniconda3/envs/qiime2-2021.2/lib/python3.6/subprocess.py", line 438, in run
    output=stdout, stderr=stderr)
subprocess.CalledProcessError: Command '['run_aldex2.R', '/tmp/tmp13arye_q/input.tsv.biom', '/tmp/tmp13arye_q/input.map.txt', 'UV', '128', 'glm', 'all', '/tmp/tmp13arye_q/output.summary.txt']' returned non-zero exit status 1.

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/home/bt140047/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/q2cli/commands.py", line 329, in __call__
    results = action(**arguments)
  File "<decorator-gen-556>", line 2, in aldex2
  File "/home/bt140047/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/qiime2/sdk/action.py", line 245, in bound_callable
    output_types, provenance)
  File "/home/bt140047/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/qiime2/sdk/action.py", line 390, in _callable_executor_
    output_views = self._callable(**view_args)
  File "/home/bt140047/miniconda3/envs/qiime2-2021.2/lib/python3.6/site-packages/q2_aldex2/_method.py", line 63, in aldex2
    " and stderr to learn more." % e.returncode)
Exception: An error was encountered while running ALDEx2 in R (return code 1), please inspect stdout and stderr to learn more.