INFO(140086093551360)2019-08-09 14:08:58,798:*************************
INFO(140086093551360)2019-08-09 14:08:58,798:deblurring started
WARNING(140086093551360)2019-08-09 14:08:58,798:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data
WARNING(140086093551360)2019-08-09 14:08:58,799:parameters: {'mean_error': 0.005, 'indel_max': 3, 'seqs_fp': '/tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data', 'overwrite': True, 'neg_ref_db_fp': (), 'indel_prob': 0.01, 'neg_ref_fp': (), 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'min_reads': 10, 'logger': <logging.Logger object at 0x7f68389b6c50>, 'pos_ref_db_fp': (), 'trim_length': 280, 'log_level': 2, 'min_size': 2, 'is_worker_thread': None, 'threads_per_sample': 1, 'left_trim_length': 0, 'pos_ref_fp': (), 'jobs_to_start': 1, 'output_dir': '/tmp/tmpefmj40f_', 'log_file': '/home/faizansaleem/Desktop/faizan/agha_khan/qiime2/deblur.log', 'keep_tmp_files': True}
INFO(140086093551360)2019-08-09 14:08:58,799:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005]
INFO(140086093551360)2019-08-09 14:08:58,799:deblur main program started
INFO(140086093551360)2019-08-09 14:08:58,805:processing directory /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data
INFO(140086093551360)2019-08-09 14:08:58,817:building negative db sortmerna index files
INFO(140086093551360)2019-08-09 14:08:58,817:build_index_sortmerna files ['/home/faizansaleem/miniconda3/envs/qiime2-2018.11/lib/python3.5/site-packages/deblur/support_files/artifacts.fa'] to dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:09:00,235:building positive db sortmerna index files
INFO(140086093551360)2019-08-09 14:09:00,235:build_index_sortmerna files ['/home/faizansaleem/miniconda3/envs/qiime2-2018.11/lib/python3.5/site-packages/deblur/support_files/88_otus.fasta'] to dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:11:10,836:processing per sample fasta files
INFO(140086093551360)2019-08-09 14:11:10,836:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:11:10,837:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/421-20-69_102_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:11:38,322:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-20-69_102_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:11:38,576:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-20-69_102_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:11:40,944:total sequences 2690, passing sequences 2690, failing sequences 0
INFO(140086093551360)2019-08-09 14:11:40,945:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-20-69_102_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:11:58,650:deblurring 2690 sequences
INFO(140086093551360)2019-08-09 14:12:04,465:876 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:12:04,474:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-20-69_102_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:12:05,672:finished processing file
INFO(140086093551360)2019-08-09 14:12:05,674:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:12:05,674:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/420-44-04_100_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:12:29,067:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-44-04_100_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:12:29,710:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-44-04_100_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:12:31,728:total sequences 2216, passing sequences 2216, failing sequences 0
INFO(140086093551360)2019-08-09 14:12:31,728:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-44-04_100_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:12:44,042:deblurring 2216 sequences
INFO(140086093551360)2019-08-09 14:12:47,647:590 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:12:47,656:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-44-04_100_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:12:48,428:finished processing file
INFO(140086093551360)2019-08-09 14:12:48,430:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:12:48,430:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/419-01-23_82_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:13:03,488:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-01-23_82_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:13:03,570:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-01-23_82_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:13:05,433:total sequences 1707, passing sequences 1707, failing sequences 0
INFO(140086093551360)2019-08-09 14:13:05,434:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-01-23_82_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:13:13,248:deblurring 1707 sequences
INFO(140086093551360)2019-08-09 14:13:16,852:879 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:13:16,860:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-01-23_82_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:13:18,064:finished processing file
INFO(140086093551360)2019-08-09 14:13:18,066:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:13:18,066:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/028-28-99_1_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:13:44,520:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/028-28-99_1_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:13:44,645:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/028-28-99_1_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:13:46,665:total sequences 2803, passing sequences 2803, failing sequences 0
INFO(140086093551360)2019-08-09 14:13:46,665:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/028-28-99_1_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:14:04,119:deblurring 2803 sequences
INFO(140086093551360)2019-08-09 14:14:11,784:1120 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:14:11,796:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/028-28-99_1_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:14:13,397:finished processing file
INFO(140086093551360)2019-08-09 14:14:13,400:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:14:13,400:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/182_77_30D_1_22_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:14:44,585:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_77_30D_1_22_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:14:44,723:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_77_30D_1_22_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:14:46,659:total sequences 2128, passing sequences 2128, failing sequences 0
INFO(140086093551360)2019-08-09 14:14:46,659:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_77_30D_1_22_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:14:58,207:deblurring 2128 sequences
INFO(140086093551360)2019-08-09 14:15:01,075:595 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:15:01,081:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_77_30D_1_22_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:15:01,909:finished processing file
INFO(140086093551360)2019-08-09 14:15:01,911:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:15:01,911:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/192-24-63_27_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:15:19,149:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/192-24-63_27_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:15:19,220:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/192-24-63_27_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:15:21,089:total sequences 1809, passing sequences 1809, failing sequences 0
INFO(140086093551360)2019-08-09 14:15:21,090:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/192-24-63_27_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:15:29,905:deblurring 1809 sequences
INFO(140086093551360)2019-08-09 14:15:32,036:412 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:15:32,042:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/192-24-63_27_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:15:32,579:finished processing file
INFO(140086093551360)2019-08-09 14:15:32,580:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:15:32,580:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/212-37-55_31_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:15:45,343:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/212-37-55_31_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:15:45,401:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/212-37-55_31_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:15:47,171:total sequences 1275, passing sequences 1275, failing sequences 0
INFO(140086093551360)2019-08-09 14:15:47,172:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/212-37-55_31_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:15:52,262:deblurring 1275 sequences
INFO(140086093551360)2019-08-09 14:15:53,592:378 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:15:53,596:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/212-37-55_31_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:15:54,080:finished processing file
INFO(140086093551360)2019-08-09 14:15:54,081:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:15:54,081:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/419-01-51_83_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:16:25,560:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-01-51_83_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:16:25,695:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-01-51_83_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:16:27,789:total sequences 3365, passing sequences 3365, failing sequences 0
INFO(140086093551360)2019-08-09 14:16:27,789:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-01-51_83_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:16:51,844:deblurring 3365 sequences
INFO(140086093551360)2019-08-09 14:16:54,978:285 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:16:54,982:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-01-51_83_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:16:55,356:finished processing file
INFO(140086093551360)2019-08-09 14:16:55,357:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:16:55,357:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/410-86-22_59_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:17:35,193:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/410-86-22_59_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:17:35,358:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/410-86-22_59_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:17:37,683:total sequences 4787, passing sequences 4787, failing sequences 0
INFO(140086093551360)2019-08-09 14:17:37,683:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/410-86-22_59_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:18:24,756:deblurring 4787 sequences
INFO(140086093551360)2019-08-09 14:18:32,800:576 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:18:32,809:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/410-86-22_59_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:18:33,584:finished processing file
INFO(140086093551360)2019-08-09 14:18:33,587:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:18:33,587:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/294-37-27_53_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:19:16,055:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/294-37-27_53_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:19:16,228:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/294-37-27_53_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:19:18,598:total sequences 5122, passing sequences 5122, failing sequences 0
INFO(140086093551360)2019-08-09 14:19:18,598:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/294-37-27_53_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:20:12,438:deblurring 5122 sequences
INFO(140086093551360)2019-08-09 14:20:20,539:539 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:20:20,547:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/294-37-27_53_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:20:21,283:finished processing file
INFO(140086093551360)2019-08-09 14:20:21,286:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:20:21,286:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/283-04-52_50_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:20:22,438:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/283-04-52_50_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:20:22,448:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/283-04-52_50_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:20:24,040:total sequences 132, passing sequences 132, failing sequences 0
INFO(140086093551360)2019-08-09 14:20:24,040:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/283-04-52_50_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:20:35,571:deblurring 132 sequences
INFO(140086093551360)2019-08-09 14:20:35,608:101 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:20:35,609:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/283-04-52_50_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:20:35,719:finished processing file
INFO(140086093551360)2019-08-09 14:20:35,720:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:20:35,720:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/290-41-61_52_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:21:05,979:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/290-41-61_52_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:21:06,111:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/290-41-61_52_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:21:08,189:total sequences 3281, passing sequences 3281, failing sequences 0
INFO(140086093551360)2019-08-09 14:21:08,189:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/290-41-61_52_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:21:31,071:deblurring 3281 sequences
INFO(140086093551360)2019-08-09 14:21:40,885:1209 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:21:40,899:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/290-41-61_52_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:21:42,592:finished processing file
INFO(140086093551360)2019-08-09 14:21:42,595:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:21:42,595:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/252-63-40_42_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:22:09,011:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/252-63-40_42_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:22:09,128:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/252-63-40_42_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:22:11,118:total sequences 2691, passing sequences 2691, failing sequences 0
INFO(140086093551360)2019-08-09 14:22:11,119:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/252-63-40_42_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:22:27,559:deblurring 2691 sequences
INFO(140086093551360)2019-08-09 14:22:31,246:517 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:22:31,252:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/252-63-40_42_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:22:31,908:finished processing file
INFO(140086093551360)2019-08-09 14:22:31,910:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:22:31,910:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/207-11-27_30_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:23:02,287:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/207-11-27_30_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:23:02,421:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/207-11-27_30_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:23:04,496:total sequences 3210, passing sequences 3210, failing sequences 0
INFO(140086093551360)2019-08-09 14:23:04,496:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/207-11-27_30_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:23:25,984:deblurring 3210 sequences
INFO(140086093551360)2019-08-09 14:23:35,256:1176 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:23:35,268:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/207-11-27_30_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:23:36,927:finished processing file
INFO(140086093551360)2019-08-09 14:23:36,929:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:23:36,930:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/257-09-59_44_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:24:09,591:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/257-09-59_44_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:24:09,733:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/257-09-59_44_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:24:11,823:total sequences 3353, passing sequences 3353, failing sequences 0
INFO(140086093551360)2019-08-09 14:24:11,823:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/257-09-59_44_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:24:35,593:deblurring 3353 sequences
INFO(140086093551360)2019-08-09 14:24:46,552:1174 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:24:46,569:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/257-09-59_44_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:24:48,264:finished processing file
INFO(140086093551360)2019-08-09 14:24:48,267:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:24:48,268:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/418-55-87_75_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:25:45,238:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-55-87_75_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:25:45,513:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-55-87_75_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:25:48,125:total sequences 6629, passing sequences 6629, failing sequences 0
INFO(140086093551360)2019-08-09 14:25:48,125:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-55-87_75_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:27:14,327:deblurring 6629 sequences
INFO(140086093551360)2019-08-09 14:27:32,907:1104 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:27:32,920:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-55-87_75_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:27:34,493:finished processing file
INFO(140086093551360)2019-08-09 14:27:34,497:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:27:34,497:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/419-53-37_88_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:27:57,241:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-53-37_88_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:27:57,335:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-53-37_88_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:27:59,302:total sequences 2486, passing sequences 2486, failing sequences 0
INFO(140086093551360)2019-08-09 14:27:59,303:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-53-37_88_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:28:13,391:deblurring 2486 sequences
INFO(140086093551360)2019-08-09 14:28:19,475:973 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:28:19,485:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-53-37_88_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:28:20,838:finished processing file
INFO(140086093551360)2019-08-09 14:28:20,840:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:28:20,840:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/242_73_43_1_40_L001_R1_001.fastq.gz
WARNING(140086093551360)2019-08-09 14:28:54,138:Vast majority of sequences (62340 / 62340) are shorter than the trim length (280). Are you using the correct -t trim length?
INFO(140086093551360)2019-08-09 14:28:54,158:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/242_73_43_1_40_L001_R1_001.fastq.gz.trim
ERROR(140086093551360)2019-08-09 14:28:54,165:Problem running vsearch dereplication on file /tmp/tmpefmj40f_/deblur_working_dir/242_73_43_1_40_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:28:54,165:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/242_73_43_1_40_L001_R1_001.fastq.gz.trim.derep
WARNING(140086093551360)2019-08-09 14:28:54,165:file /tmp/tmpefmj40f_/deblur_working_dir/242_73_43_1_40_L001_R1_001.fastq.gz.trim.derep has size 0, continuing
WARNING(140086093551360)2019-08-09 14:28:54,165:remove artifacts failed, aborting
WARNING(140086093551360)2019-08-09 14:28:54,166:deblurring failed for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/242_73_43_1_40_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:28:54,166:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:28:54,166:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/298-73-74_56_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:29:46,937:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/298-73-74_56_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:29:47,179:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/298-73-74_56_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:29:49,515:total sequences 4939, passing sequences 4939, failing sequences 0
INFO(140086093551360)2019-08-09 14:29:49,515:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/298-73-74_56_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:30:37,953:deblurring 4939 sequences
INFO(140086093551360)2019-08-09 14:30:49,286:802 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:30:49,296:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/298-73-74_56_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:30:50,398:finished processing file
INFO(140086093551360)2019-08-09 14:30:50,401:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:30:50,401:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/101_71_34_1_10_L001_R1_001.fastq.gz
WARNING(140086093551360)2019-08-09 14:31:23,415:Vast majority of sequences (61760 / 61760) are shorter than the trim length (280). Are you using the correct -t trim length?
INFO(140086093551360)2019-08-09 14:31:23,415:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/101_71_34_1_10_L001_R1_001.fastq.gz.trim
ERROR(140086093551360)2019-08-09 14:31:23,423:Problem running vsearch dereplication on file /tmp/tmpefmj40f_/deblur_working_dir/101_71_34_1_10_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:31:23,424:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/101_71_34_1_10_L001_R1_001.fastq.gz.trim.derep
WARNING(140086093551360)2019-08-09 14:31:23,424:file /tmp/tmpefmj40f_/deblur_working_dir/101_71_34_1_10_L001_R1_001.fastq.gz.trim.derep has size 0, continuing
WARNING(140086093551360)2019-08-09 14:31:23,424:remove artifacts failed, aborting
WARNING(140086093551360)2019-08-09 14:31:23,424:deblurring failed for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/101_71_34_1_10_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:31:23,424:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:31:23,424:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/419-24-11_85_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:32:04,399:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-24-11_85_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:32:04,570:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-24-11_85_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:32:06,823:total sequences 4315, passing sequences 4315, failing sequences 0
INFO(140086093551360)2019-08-09 14:32:06,823:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-24-11_85_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:32:43,479:deblurring 4315 sequences
INFO(140086093551360)2019-08-09 14:32:59,603:1566 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:32:59,619:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-24-11_85_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:33:01,865:finished processing file
INFO(140086093551360)2019-08-09 14:33:01,869:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:33:01,869:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/410_03_91_1_62_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:33:33,693:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_03_91_1_62_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:33:33,821:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_03_91_1_62_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:33:35,775:total sequences 2374, passing sequences 2374, failing sequences 0
INFO(140086093551360)2019-08-09 14:33:35,775:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_03_91_1_62_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:33:49,288:deblurring 2374 sequences
INFO(140086093551360)2019-08-09 14:33:53,318:755 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:33:53,326:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_03_91_1_62_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:33:54,364:finished processing file
INFO(140086093551360)2019-08-09 14:33:54,366:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:33:54,366:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/805-57-66_110_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:34:21,526:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/805-57-66_110_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:34:21,646:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/805-57-66_110_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:34:23,640:total sequences 2632, passing sequences 2632, failing sequences 0
INFO(140086093551360)2019-08-09 14:34:23,640:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/805-57-66_110_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:34:39,476:deblurring 2632 sequences
INFO(140086093551360)2019-08-09 14:34:43,746:596 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:34:43,753:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/805-57-66_110_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:34:44,553:finished processing file
INFO(140086093551360)2019-08-09 14:34:44,555:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:34:44,555:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/224-16-31_37_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:35:02,050:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/224-16-31_37_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:35:02,123:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/224-16-31_37_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:35:03,966:total sequences 1712, passing sequences 1712, failing sequences 0
INFO(140086093551360)2019-08-09 14:35:03,966:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/224-16-31_37_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:35:11,947:deblurring 1712 sequences
INFO(140086093551360)2019-08-09 14:35:13,600:348 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:35:13,605:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/224-16-31_37_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:35:14,058:finished processing file
INFO(140086093551360)2019-08-09 14:35:14,059:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:35:14,059:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/418-82-21_81_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:35:29,404:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-82-21_81_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:35:29,471:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-82-21_81_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:35:31,326:total sequences 1570, passing sequences 1570, failing sequences 0
INFO(140086093551360)2019-08-09 14:35:31,326:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-82-21_81_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:35:38,123:deblurring 1570 sequences
INFO(140086093551360)2019-08-09 14:35:41,162:801 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:35:41,170:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-82-21_81_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:35:42,249:finished processing file
INFO(140086093551360)2019-08-09 14:35:42,251:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:35:42,251:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/131-29-35_12_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:36:26,390:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/131-29-35_12_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:36:26,599:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/131-29-35_12_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:36:28,908:total sequences 4608, passing sequences 4608, failing sequences 0
INFO(140086093551360)2019-08-09 14:36:28,908:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/131-29-35_12_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:37:10,558:deblurring 4608 sequences
INFO(140086093551360)2019-08-09 14:37:28,375:1555 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:37:28,396:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/131-29-35_12_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:37:30,608:finished processing file
INFO(140086093551360)2019-08-09 14:37:30,612:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:37:30,612:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/421-21-74_103_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:38:10,325:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-21-74_103_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:38:10,496:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-21-74_103_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:38:12,729:total sequences 4100, passing sequences 4100, failing sequences 0
INFO(140086093551360)2019-08-09 14:38:12,729:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-21-74_103_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:38:46,822:deblurring 4100 sequences
INFO(140086093551360)2019-08-09 14:38:57,583:1037 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:38:57,595:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-21-74_103_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:38:59,056:finished processing file
INFO(140086093551360)2019-08-09 14:38:59,059:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:38:59,059:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/206-70-38_29_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:39:41,398:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/206-70-38_29_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:39:41,580:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/206-70-38_29_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:39:43,796:total sequences 4107, passing sequences 4107, failing sequences 0
INFO(140086093551360)2019-08-09 14:39:43,796:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/206-70-38_29_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:40:18,604:deblurring 4107 sequences
INFO(140086093551360)2019-08-09 14:40:28,198:921 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:40:28,211:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/206-70-38_29_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:40:29,454:finished processing file
INFO(140086093551360)2019-08-09 14:40:29,457:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:40:29,457:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/185-69-58_26_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:40:56,611:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/185-69-58_26_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:40:56,748:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/185-69-58_26_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:40:58,785:total sequences 2845, passing sequences 2845, failing sequences 0
INFO(140086093551360)2019-08-09 14:40:58,785:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/185-69-58_26_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:41:17,184:deblurring 2845 sequences
INFO(140086093551360)2019-08-09 14:41:24,842:999 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:41:24,857:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/185-69-58_26_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:41:26,206:finished processing file
INFO(140086093551360)2019-08-09 14:41:26,209:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:41:26,209:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/417-81-47_73_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:41:49,708:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-81-47_73_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:41:49,802:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-81-47_73_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:41:51,778:total sequences 2449, passing sequences 2449, failing sequences 0
INFO(140086093551360)2019-08-09 14:41:51,778:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-81-47_73_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:42:06,140:deblurring 2449 sequences
INFO(140086093551360)2019-08-09 14:42:09,047:427 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:42:09,055:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-81-47_73_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:42:09,610:finished processing file
INFO(140086093551360)2019-08-09 14:42:09,611:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:42:09,612:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/410-97-05_60_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:42:27,257:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/410-97-05_60_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:42:27,334:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/410-97-05_60_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:42:29,201:total sequences 1816, passing sequences 1816, failing sequences 0
INFO(140086093551360)2019-08-09 14:42:29,201:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/410-97-05_60_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:42:37,814:deblurring 1816 sequences
INFO(140086093551360)2019-08-09 14:42:41,386:729 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:42:41,393:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/410-97-05_60_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:42:42,386:finished processing file
INFO(140086093551360)2019-08-09 14:42:42,388:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:42:42,388:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/155-36-47_15_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:43:18,877:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/155-36-47_15_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:43:19,031:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/155-36-47_15_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:43:21,222:total sequences 3740, passing sequences 3740, failing sequences 0
INFO(140086093551360)2019-08-09 14:43:21,223:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/155-36-47_15_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:43:49,669:deblurring 3740 sequences
INFO(140086093551360)2019-08-09 14:44:03,219:1544 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:44:03,235:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/155-36-47_15_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:44:05,405:finished processing file
INFO(140086093551360)2019-08-09 14:44:05,408:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:44:05,408:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/420-05-51_95_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:44:32,427:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-05-51_95_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:44:32,552:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-05-51_95_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:44:34,622:total sequences 2976, passing sequences 2976, failing sequences 0
INFO(140086093551360)2019-08-09 14:44:34,622:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-05-51_95_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:44:53,520:deblurring 2976 sequences
INFO(140086093551360)2019-08-09 14:45:01,743:1147 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:45:01,754:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-05-51_95_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:45:03,360:finished processing file
INFO(140086093551360)2019-08-09 14:45:03,362:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:45:03,363:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/265-00-60_46_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:45:19,676:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/265-00-60_46_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:45:19,745:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/265-00-60_46_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:45:21,581:total sequences 1649, passing sequences 1649, failing sequences 0
INFO(140086093551360)2019-08-09 14:45:21,581:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/265-00-60_46_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:45:28,947:deblurring 1649 sequences
INFO(140086093551360)2019-08-09 14:45:30,234:251 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:45:30,237:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/265-00-60_46_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:45:30,536:finished processing file
INFO(140086093551360)2019-08-09 14:45:30,538:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:45:30,538:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/183-91-76_24_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:46:11,335:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/183-91-76_24_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:46:11,506:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/183-91-76_24_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:46:13,724:total sequences 3900, passing sequences 3900, failing sequences 0
INFO(140086093551360)2019-08-09 14:46:13,724:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/183-91-76_24_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:46:45,449:deblurring 3900 sequences
INFO(140086093551360)2019-08-09 14:46:54,397:858 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:46:54,408:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/183-91-76_24_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:46:55,589:finished processing file
INFO(140086093551360)2019-08-09 14:46:55,591:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:46:55,592:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/418-05-41_74_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:47:11,150:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-05-41_74_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:47:11,244:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-05-41_74_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:47:13,083:total sequences 1506, passing sequences 1506, failing sequences 0
INFO(140086093551360)2019-08-09 14:47:13,083:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-05-41_74_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:47:19,582:deblurring 1506 sequences
INFO(140086093551360)2019-08-09 14:47:21,645:531 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:47:21,652:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-05-41_74_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:47:22,351:finished processing file
INFO(140086093551360)2019-08-09 14:47:22,353:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:47:22,353:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/419-24-40_86_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:47:49,892:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-24-40_86_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:47:50,018:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-24-40_86_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:47:52,110:total sequences 2862, passing sequences 2862, failing sequences 0
INFO(140086093551360)2019-08-09 14:47:52,110:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-24-40_86_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:48:10,438:deblurring 2862 sequences
INFO(140086093551360)2019-08-09 14:48:18,097:1084 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:48:18,112:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-24-40_86_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:48:19,596:finished processing file
INFO(140086093551360)2019-08-09 14:48:19,598:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:48:19,599:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/418-57-61_76_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:49:17,443:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-57-61_76_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:49:17,711:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-57-61_76_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:49:20,163:total sequences 5466, passing sequences 5466, failing sequences 0
INFO(140086093551360)2019-08-09 14:49:20,163:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-57-61_76_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:50:19,233:deblurring 5466 sequences
INFO(140086093551360)2019-08-09 14:50:44,739:1943 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:50:44,765:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-57-61_76_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:50:47,523:finished processing file
INFO(140086093551360)2019-08-09 14:50:47,529:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:50:47,530:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/418-74-21_78_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:51:20,245:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-74-21_78_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:51:20,404:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-74-21_78_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:51:22,507:total sequences 3288, passing sequences 3288, failing sequences 0
INFO(140086093551360)2019-08-09 14:51:22,507:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-74-21_78_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:51:45,954:deblurring 3288 sequences
INFO(140086093551360)2019-08-09 14:51:52,629:808 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:51:52,641:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-74-21_78_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:51:53,712:finished processing file
INFO(140086093551360)2019-08-09 14:51:53,715:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:51:53,715:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/420-26-77_98_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:52:11,542:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-26-77_98_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:52:11,616:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-26-77_98_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:52:13,482:total sequences 1682, passing sequences 1682, failing sequences 0
INFO(140086093551360)2019-08-09 14:52:13,483:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-26-77_98_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:52:20,936:deblurring 1682 sequences
INFO(140086093551360)2019-08-09 14:52:22,873:383 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:52:22,877:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-26-77_98_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:52:23,423:finished processing file
INFO(140086093551360)2019-08-09 14:52:23,424:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:52:23,425:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/608-30-99_107_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:53:06,328:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/608-30-99_107_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:53:06,550:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/608-30-99_107_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:53:08,818:total sequences 4377, passing sequences 4377, failing sequences 0
INFO(140086093551360)2019-08-09 14:53:08,818:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/608-30-99_107_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:53:50,911:deblurring 4377 sequences
INFO(140086093551360)2019-08-09 14:54:04,328:1274 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:54:04,343:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/608-30-99_107_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:54:06,148:finished processing file
INFO(140086093551360)2019-08-09 14:54:06,151:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:54:06,151:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/411_21_54_1_67_L001_R1_001.fastq.gz
WARNING(140086093551360)2019-08-09 14:54:39,821:Vast majority of sequences (62340 / 62340) are shorter than the trim length (280). Are you using the correct -t trim length?
INFO(140086093551360)2019-08-09 14:54:39,821:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/411_21_54_1_67_L001_R1_001.fastq.gz.trim
ERROR(140086093551360)2019-08-09 14:54:39,829:Problem running vsearch dereplication on file /tmp/tmpefmj40f_/deblur_working_dir/411_21_54_1_67_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:54:39,829:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/411_21_54_1_67_L001_R1_001.fastq.gz.trim.derep
WARNING(140086093551360)2019-08-09 14:54:39,829:file /tmp/tmpefmj40f_/deblur_working_dir/411_21_54_1_67_L001_R1_001.fastq.gz.trim.derep has size 0, continuing
WARNING(140086093551360)2019-08-09 14:54:39,830:remove artifacts failed, aborting
WARNING(140086093551360)2019-08-09 14:54:39,830:deblurring failed for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/411_21_54_1_67_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:54:39,830:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:54:39,830:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/163-01-94_16_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:55:07,313:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/163-01-94_16_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:55:07,438:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/163-01-94_16_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:55:09,520:total sequences 2957, passing sequences 2957, failing sequences 0
INFO(140086093551360)2019-08-09 14:55:09,520:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/163-01-94_16_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:55:29,618:deblurring 2957 sequences
INFO(140086093551360)2019-08-09 14:55:34,527:605 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:55:34,536:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/163-01-94_16_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:55:35,310:finished processing file
INFO(140086093551360)2019-08-09 14:55:35,313:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:55:35,313:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/417-65-73_71_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:56:09,926:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-65-73_71_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:56:10,095:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-65-73_71_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:56:12,279:total sequences 3717, passing sequences 3717, failing sequences 0
INFO(140086093551360)2019-08-09 14:56:12,279:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-65-73_71_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:56:43,450:deblurring 3717 sequences
INFO(140086093551360)2019-08-09 14:56:50,561:753 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:56:50,570:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-65-73_71_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:56:51,719:finished processing file
INFO(140086093551360)2019-08-09 14:56:51,722:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:56:51,722:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/802-64-35_109_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:57:21,293:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/802-64-35_109_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:57:21,428:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/802-64-35_109_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:57:23,549:total sequences 3046, passing sequences 3046, failing sequences 0
INFO(140086093551360)2019-08-09 14:57:23,550:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/802-64-35_109_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:57:44,732:deblurring 3046 sequences
INFO(140086093551360)2019-08-09 14:57:52,642:1047 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:57:52,654:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/802-64-35_109_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:57:54,139:finished processing file
INFO(140086093551360)2019-08-09 14:57:54,141:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:57:54,141:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/182_01_01D_1_20_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:58:26,202:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_01_01D_1_20_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:58:26,328:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_01_01D_1_20_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:58:28,230:total sequences 2022, passing sequences 2022, failing sequences 0
INFO(140086093551360)2019-08-09 14:58:28,230:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_01_01D_1_20_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:58:39,105:deblurring 2022 sequences
INFO(140086093551360)2019-08-09 14:58:41,843:598 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:58:41,849:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_01_01D_1_20_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:58:42,671:finished processing file
INFO(140086093551360)2019-08-09 14:58:42,672:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:58:42,673:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/182_01_01_1_21_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 14:59:14,481:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_01_01_1_21_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 14:59:14,606:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_01_01_1_21_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 14:59:16,653:total sequences 1858, passing sequences 1858, failing sequences 0
INFO(140086093551360)2019-08-09 14:59:16,654:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_01_01_1_21_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 14:59:26,204:deblurring 1858 sequences
INFO(140086093551360)2019-08-09 14:59:28,816:624 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 14:59:28,822:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_01_01_1_21_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 14:59:29,698:finished processing file
INFO(140086093551360)2019-08-09 14:59:29,699:--------------------------------------------------------
INFO(140086093551360)2019-08-09 14:59:29,699:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/420-40-58_99_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:00:15,925:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-40-58_99_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:00:16,145:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-40-58_99_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:00:18,783:total sequences 4626, passing sequences 4626, failing sequences 0
INFO(140086093551360)2019-08-09 15:00:18,783:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-40-58_99_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:01:08,187:deblurring 4626 sequences
INFO(140086093551360)2019-08-09 15:01:27,444:1766 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:01:27,467:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-40-58_99_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:01:30,045:finished processing file
INFO(140086093551360)2019-08-09 15:01:30,050:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:01:30,050:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/296-66-86_54_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:01:48,898:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/296-66-86_54_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:01:48,988:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/296-66-86_54_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:01:50,879:total sequences 1982, passing sequences 1982, failing sequences 0
INFO(140086093551360)2019-08-09 15:01:50,880:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/296-66-86_54_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:02:00,922:deblurring 1982 sequences
INFO(140086093551360)2019-08-09 15:02:03,092:360 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:02:03,096:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/296-66-86_54_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:02:03,557:finished processing file
INFO(140086093551360)2019-08-09 15:02:03,559:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:02:03,559:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/418-77-98_80_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:02:23,466:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-77-98_80_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:02:23,561:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-77-98_80_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:02:25,474:total sequences 2046, passing sequences 2046, failing sequences 0
INFO(140086093551360)2019-08-09 15:02:25,474:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-77-98_80_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:02:36,083:deblurring 2046 sequences
INFO(140086093551360)2019-08-09 15:02:39,013:564 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:02:39,019:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-77-98_80_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:02:39,795:finished processing file
INFO(140086093551360)2019-08-09 15:02:39,796:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:02:39,796:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/182_77_30_1_23_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:03:03,486:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_77_30_1_23_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:03:03,583:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_77_30_1_23_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:03:05,485:total sequences 2103, passing sequences 2103, failing sequences 0
INFO(140086093551360)2019-08-09 15:03:05,485:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_77_30_1_23_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:03:17,092:deblurring 2103 sequences
INFO(140086093551360)2019-08-09 15:03:18,954:392 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:03:18,959:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/182_77_30_1_23_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:03:19,481:finished processing file
INFO(140086093551360)2019-08-09 15:03:19,482:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:03:19,482:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/213-91-44_32_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:04:14,409:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/213-91-44_32_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:04:15,244:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/213-91-44_32_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:04:17,862:total sequences 6065, passing sequences 6065, failing sequences 0
INFO(140086093551360)2019-08-09 15:04:17,862:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/213-91-44_32_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:05:37,314:deblurring 6065 sequences
INFO(140086093551360)2019-08-09 15:06:04,377:1864 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:06:04,403:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/213-91-44_32_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:06:07,250:finished processing file
INFO(140086093551360)2019-08-09 15:06:07,256:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:06:07,256:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/420-68-05_101_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:06:31,525:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-68-05_101_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:06:31,635:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-68-05_101_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:06:33,625:total sequences 2644, passing sequences 2644, failing sequences 0
INFO(140086093551360)2019-08-09 15:06:33,626:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-68-05_101_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:06:49,901:deblurring 2644 sequences
INFO(140086093551360)2019-08-09 15:06:53,186:462 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:06:53,192:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-68-05_101_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:06:53,789:finished processing file
INFO(140086093551360)2019-08-09 15:06:53,792:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:06:53,792:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/218-83-55_34_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:07:05,181:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/218-83-55_34_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:07:05,229:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/218-83-55_34_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:07:06,992:total sequences 1229, passing sequences 1229, failing sequences 0
INFO(140086093551360)2019-08-09 15:07:06,992:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/218-83-55_34_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:07:11,808:deblurring 1229 sequences
INFO(140086093551360)2019-08-09 15:07:13,423:488 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:07:13,428:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/218-83-55_34_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:07:14,093:finished processing file
INFO(140086093551360)2019-08-09 15:07:14,095:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:07:14,095:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/421-25-37_104_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:07:36,868:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-25-37_104_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:07:36,958:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-25-37_104_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:07:38,895:total sequences 2296, passing sequences 2296, failing sequences 0
INFO(140086093551360)2019-08-09 15:07:38,895:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-25-37_104_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:07:51,632:deblurring 2296 sequences
INFO(140086093551360)2019-08-09 15:07:54,574:479 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:07:54,579:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-25-37_104_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:07:55,200:finished processing file
INFO(140086093551360)2019-08-09 15:07:55,202:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:07:55,202:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/177-86-50_18_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:08:24,890:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/177-86-50_18_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:08:25,029:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/177-86-50_18_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:08:27,099:total sequences 3059, passing sequences 3059, failing sequences 0
INFO(140086093551360)2019-08-09 15:08:27,099:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/177-86-50_18_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:08:47,394:deblurring 3059 sequences
INFO(140086093551360)2019-08-09 15:08:54,813:898 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:08:54,824:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/177-86-50_18_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:08:56,091:finished processing file
INFO(140086093551360)2019-08-09 15:08:56,093:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:08:56,094:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/135_77_01_1_14_L001_R1_001.fastq.gz
WARNING(140086093551360)2019-08-09 15:09:29,781:Vast majority of sequences (62940 / 62940) are shorter than the trim length (280). Are you using the correct -t trim length?
INFO(140086093551360)2019-08-09 15:09:29,781:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/135_77_01_1_14_L001_R1_001.fastq.gz.trim
ERROR(140086093551360)2019-08-09 15:09:29,789:Problem running vsearch dereplication on file /tmp/tmpefmj40f_/deblur_working_dir/135_77_01_1_14_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:09:29,790:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/135_77_01_1_14_L001_R1_001.fastq.gz.trim.derep
WARNING(140086093551360)2019-08-09 15:09:29,790:file /tmp/tmpefmj40f_/deblur_working_dir/135_77_01_1_14_L001_R1_001.fastq.gz.trim.derep has size 0, continuing
WARNING(140086093551360)2019-08-09 15:09:29,790:remove artifacts failed, aborting
WARNING(140086093551360)2019-08-09 15:09:29,790:deblurring failed for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/135_77_01_1_14_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:09:29,790:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:09:29,790:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/128_78_28_1_11_L001_R1_001.fastq.gz
WARNING(140086093551360)2019-08-09 15:10:02,797:Vast majority of sequences (61700 / 61700) are shorter than the trim length (280). Are you using the correct -t trim length?
INFO(140086093551360)2019-08-09 15:10:02,797:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/128_78_28_1_11_L001_R1_001.fastq.gz.trim
ERROR(140086093551360)2019-08-09 15:10:02,804:Problem running vsearch dereplication on file /tmp/tmpefmj40f_/deblur_working_dir/128_78_28_1_11_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:10:02,804:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/128_78_28_1_11_L001_R1_001.fastq.gz.trim.derep
WARNING(140086093551360)2019-08-09 15:10:02,805:file /tmp/tmpefmj40f_/deblur_working_dir/128_78_28_1_11_L001_R1_001.fastq.gz.trim.derep has size 0, continuing
WARNING(140086093551360)2019-08-09 15:10:02,805:remove artifacts failed, aborting
WARNING(140086093551360)2019-08-09 15:10:02,805:deblurring failed for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/128_78_28_1_11_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:10:02,805:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:10:02,805:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/418-60-22_77_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:10:38,292:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-60-22_77_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:10:38,436:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-60-22_77_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:10:40,520:total sequences 3120, passing sequences 3120, failing sequences 0
INFO(140086093551360)2019-08-09 15:10:40,520:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-60-22_77_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:11:02,220:deblurring 3120 sequences
INFO(140086093551360)2019-08-09 15:11:10,277:1089 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:11:10,289:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-60-22_77_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:11:11,775:finished processing file
INFO(140086093551360)2019-08-09 15:11:11,777:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:11:11,777:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/245-17-65_41_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:11:34,374:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/245-17-65_41_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:11:34,464:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/245-17-65_41_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:11:36,470:total sequences 2352, passing sequences 2352, failing sequences 0
INFO(140086093551360)2019-08-09 15:11:36,470:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/245-17-65_41_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:11:49,919:deblurring 2352 sequences
INFO(140086093551360)2019-08-09 15:11:53,495:598 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:11:53,502:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/245-17-65_41_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:11:54,267:finished processing file
INFO(140086093551360)2019-08-09 15:11:54,269:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:11:54,269:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/420-11-29_96_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:12:03,567:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-11-29_96_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:12:03,640:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-11-29_96_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:12:05,397:total sequences 829, passing sequences 829, failing sequences 0
INFO(140086093551360)2019-08-09 15:12:05,397:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-11-29_96_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:12:08,353:deblurring 829 sequences
INFO(140086093551360)2019-08-09 15:12:08,910:225 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:12:08,914:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-11-29_96_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:12:09,218:finished processing file
INFO(140086093551360)2019-08-09 15:12:09,219:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:12:09,219:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/297-54-08_55_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:13:01,094:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/297-54-08_55_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:13:01,425:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/297-54-08_55_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:13:03,758:total sequences 4253, passing sequences 4253, failing sequences 0
INFO(140086093551360)2019-08-09 15:13:03,758:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/297-54-08_55_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:13:46,410:deblurring 4253 sequences
INFO(140086093551360)2019-08-09 15:13:53,893:651 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:13:53,903:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/297-54-08_55_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:13:54,753:finished processing file
INFO(140086093551360)2019-08-09 15:13:54,756:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:13:54,756:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/417-50-76_70_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:14:12,122:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-50-76_70_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:14:12,195:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-50-76_70_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:14:14,051:total sequences 1727, passing sequences 1727, failing sequences 0
INFO(140086093551360)2019-08-09 15:14:14,051:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-50-76_70_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:14:21,956:deblurring 1727 sequences
INFO(140086093551360)2019-08-09 15:14:24,748:624 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:14:24,756:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-50-76_70_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:14:25,573:finished processing file
INFO(140086093551360)2019-08-09 15:14:25,575:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:14:25,575:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/167-40-79_17_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:14:50,230:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/167-40-79_17_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:14:50,357:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/167-40-79_17_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:14:52,425:total sequences 2690, passing sequences 2690, failing sequences 0
INFO(140086093551360)2019-08-09 15:14:52,425:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/167-40-79_17_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:15:08,735:deblurring 2690 sequences
INFO(140086093551360)2019-08-09 15:15:14,385:813 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:15:14,396:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/167-40-79_17_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:15:15,539:finished processing file
INFO(140086093551360)2019-08-09 15:15:15,541:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:15:15,541:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/090-22-86_9_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:15:29,265:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/090-22-86_9_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:15:29,522:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/090-22-86_9_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:15:31,747:total sequences 1452, passing sequences 1452, failing sequences 0
INFO(140086093551360)2019-08-09 15:15:31,747:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/090-22-86_9_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:15:37,859:deblurring 1452 sequences
INFO(140086093551360)2019-08-09 15:15:39,907:544 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:15:39,912:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/090-22-86_9_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:15:40,647:finished processing file
INFO(140086093551360)2019-08-09 15:15:40,649:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:15:40,649:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/419-53-46_89_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:16:50,370:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-53-46_89_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:16:50,939:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-53-46_89_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:16:53,952:total sequences 8353, passing sequences 8353, failing sequences 0
INFO(140086093551360)2019-08-09 15:16:53,952:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-53-46_89_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:19:24,400:deblurring 8353 sequences
INFO(140086093551360)2019-08-09 15:19:41,679:765 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:19:41,692:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-53-46_89_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:19:42,729:finished processing file
INFO(140086093551360)2019-08-09 15:19:42,733:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:19:42,733:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/066-18-88_5_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:20:00,030:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/066-18-88_5_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:20:00,117:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/066-18-88_5_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:20:01,983:total sequences 1772, passing sequences 1772, failing sequences 0
INFO(140086093551360)2019-08-09 15:20:01,983:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/066-18-88_5_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:20:10,293:deblurring 1772 sequences
INFO(140086093551360)2019-08-09 15:20:12,683:497 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:20:12,689:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/066-18-88_5_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:20:13,327:finished processing file
INFO(140086093551360)2019-08-09 15:20:13,329:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:20:13,329:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/242-30-76_39_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:20:59,312:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/242-30-76_39_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:20:59,546:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/242-30-76_39_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:21:02,389:total sequences 4510, passing sequences 4510, failing sequences 0
INFO(140086093551360)2019-08-09 15:21:02,389:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/242-30-76_39_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:21:42,542:deblurring 4510 sequences
INFO(140086093551360)2019-08-09 15:21:59,584:1605 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:21:59,600:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/242-30-76_39_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:22:01,815:finished processing file
INFO(140086093551360)2019-08-09 15:22:01,819:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:22:01,819:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/420-04-07_94_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:22:34,577:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-04-07_94_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:22:34,719:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-04-07_94_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:22:36,827:total sequences 3389, passing sequences 3389, failing sequences 0
INFO(140086093551360)2019-08-09 15:22:36,827:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-04-07_94_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:23:01,575:deblurring 3389 sequences
INFO(140086093551360)2019-08-09 15:23:09,583:836 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:23:09,596:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-04-07_94_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:23:10,733:finished processing file
INFO(140086093551360)2019-08-09 15:23:10,736:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:23:10,736:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/030-14-82_3_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:23:19,767:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/030-14-82_3_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:23:19,807:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/030-14-82_3_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:23:21,537:total sequences 920, passing sequences 920, failing sequences 0
INFO(140086093551360)2019-08-09 15:23:21,538:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/030-14-82_3_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:23:24,676:deblurring 920 sequences
INFO(140086093551360)2019-08-09 15:23:25,723:421 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:23:25,727:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/030-14-82_3_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:23:26,268:finished processing file
INFO(140086093551360)2019-08-09 15:23:26,269:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:23:26,269:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/002-28-38_0_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:24:17,181:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/002-28-38_0_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:24:17,419:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/002-28-38_0_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:24:19,881:total sequences 5660, passing sequences 5660, failing sequences 0
INFO(140086093551360)2019-08-09 15:24:19,881:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/002-28-38_0_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:25:24,461:deblurring 5660 sequences
INFO(140086093551360)2019-08-09 15:25:33,050:417 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:25:33,059:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/002-28-38_0_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:25:33,635:finished processing file
INFO(140086093551360)2019-08-09 15:25:33,637:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:25:33,638:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/410_06_12_1_63_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:25:54,487:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_06_12_1_63_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:25:54,566:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_06_12_1_63_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:25:56,356:total sequences 1263, passing sequences 1263, failing sequences 0
INFO(140086093551360)2019-08-09 15:25:56,357:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_06_12_1_63_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:26:01,887:deblurring 1263 sequences
INFO(140086093551360)2019-08-09 15:26:03,196:467 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:26:03,203:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_06_12_1_63_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:26:03,815:finished processing file
INFO(140086093551360)2019-08-09 15:26:03,816:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:26:03,816:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/410_61_82_1_66_L001_R1_001.fastq.gz
WARNING(140086093551360)2019-08-09 15:26:36,796:Vast majority of sequences (61740 / 61740) are shorter than the trim length (280). Are you using the correct -t trim length?
INFO(140086093551360)2019-08-09 15:26:36,796:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_61_82_1_66_L001_R1_001.fastq.gz.trim
ERROR(140086093551360)2019-08-09 15:26:36,804:Problem running vsearch dereplication on file /tmp/tmpefmj40f_/deblur_working_dir/410_61_82_1_66_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:26:36,804:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_61_82_1_66_L001_R1_001.fastq.gz.trim.derep
WARNING(140086093551360)2019-08-09 15:26:36,804:file /tmp/tmpefmj40f_/deblur_working_dir/410_61_82_1_66_L001_R1_001.fastq.gz.trim.derep has size 0, continuing
WARNING(140086093551360)2019-08-09 15:26:36,804:remove artifacts failed, aborting
WARNING(140086093551360)2019-08-09 15:26:36,804:deblurring failed for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/410_61_82_1_66_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:26:36,804:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:26:36,804:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/214-28-92_33_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:27:25,666:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/214-28-92_33_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:27:25,904:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/214-28-92_33_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:27:28,219:total sequences 4792, passing sequences 4792, failing sequences 0
INFO(140086093551360)2019-08-09 15:27:28,219:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/214-28-92_33_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:28:14,224:deblurring 4792 sequences
INFO(140086093551360)2019-08-09 15:28:30,859:1329 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:28:30,878:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/214-28-92_33_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:28:32,776:finished processing file
INFO(140086093551360)2019-08-09 15:28:32,780:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:28:32,780:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/807-59-97_111_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:28:54,782:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/807-59-97_111_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:28:54,872:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/807-59-97_111_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:28:56,812:total sequences 2304, passing sequences 2304, failing sequences 0
INFO(140086093551360)2019-08-09 15:28:56,812:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/807-59-97_111_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:29:09,738:deblurring 2304 sequences
INFO(140086093551360)2019-08-09 15:29:12,891:521 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:29:12,898:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/807-59-97_111_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:29:13,562:finished processing file
INFO(140086093551360)2019-08-09 15:29:13,563:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:29:13,564:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/417-78-16_72_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:29:51,732:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-78-16_72_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:29:51,891:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-78-16_72_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:29:54,118:total sequences 4014, passing sequences 4014, failing sequences 0
INFO(140086093551360)2019-08-09 15:29:54,119:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-78-16_72_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:30:28,288:deblurring 4014 sequences
INFO(140086093551360)2019-08-09 15:30:38,730:1058 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:30:38,746:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/417-78-16_72_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:30:40,217:finished processing file
INFO(140086093551360)2019-08-09 15:30:40,220:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:30:40,221:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/419-85-52_90_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:31:20,990:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-85-52_90_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:31:21,174:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-85-52_90_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:31:23,478:total sequences 3888, passing sequences 3888, failing sequences 0
INFO(140086093551360)2019-08-09 15:31:23,479:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-85-52_90_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:31:55,947:deblurring 3888 sequences
INFO(140086093551360)2019-08-09 15:32:06,975:1038 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:32:06,990:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-85-52_90_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:32:08,415:finished processing file
INFO(140086093551360)2019-08-09 15:32:08,419:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:32:08,419:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/299-40-50_57_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:32:41,684:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/299-40-50_57_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:32:41,858:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/299-40-50_57_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:32:43,954:total sequences 3254, passing sequences 3254, failing sequences 0
INFO(140086093551360)2019-08-09 15:32:43,954:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/299-40-50_57_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:33:07,067:deblurring 3254 sequences
INFO(140086093551360)2019-08-09 15:33:13,398:758 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:33:13,431:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/299-40-50_57_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:33:14,452:finished processing file
INFO(140086093551360)2019-08-09 15:33:14,454:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:33:14,455:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/611-63-23_108_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:33:23,139:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/611-63-23_108_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:33:23,192:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/611-63-23_108_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:33:24,924:total sequences 910, passing sequences 910, failing sequences 0
INFO(140086093551360)2019-08-09 15:33:24,925:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/611-63-23_108_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:33:28,180:deblurring 910 sequences
INFO(140086093551360)2019-08-09 15:33:29,180:430 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:33:29,184:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/611-63-23_108_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:33:29,797:finished processing file
INFO(140086093551360)2019-08-09 15:33:29,798:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:33:29,798:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/418-77-62_79_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:34:10,841:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-77-62_79_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:34:11,122:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-77-62_79_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:34:13,524:total sequences 4632, passing sequences 4632, failing sequences 0
INFO(140086093551360)2019-08-09 15:34:13,524:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-77-62_79_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:35:02,401:deblurring 4632 sequences
INFO(140086093551360)2019-08-09 15:35:12,908:803 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:35:12,922:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/418-77-62_79_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:35:14,014:finished processing file
INFO(140086093551360)2019-08-09 15:35:14,017:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:35:14,018:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/075-05-46_7_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:36:10,237:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/075-05-46_7_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:36:10,487:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/075-05-46_7_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:36:13,013:total sequences 5941, passing sequences 5941, failing sequences 0
INFO(140086093551360)2019-08-09 15:36:13,014:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/075-05-46_7_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:37:30,357:deblurring 5941 sequences
INFO(140086093551360)2019-08-09 15:37:45,329:921 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:37:45,346:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/075-05-46_7_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:37:46,547:finished processing file
INFO(140086093551360)2019-08-09 15:37:46,551:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:37:46,551:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/419-94-91_93_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:38:20,378:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-94-91_93_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:38:20,523:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-94-91_93_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:38:22,669:total sequences 3625, passing sequences 3625, failing sequences 0
INFO(140086093551360)2019-08-09 15:38:22,669:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-94-91_93_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:38:49,702:deblurring 3625 sequences
INFO(140086093551360)2019-08-09 15:39:01,031:1267 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:39:01,046:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-94-91_93_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:39:02,854:finished processing file
INFO(140086093551360)2019-08-09 15:39:02,858:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:39:02,858:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/253-71-10_43_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:39:24,354:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/253-71-10_43_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:39:24,442:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/253-71-10_43_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:39:26,421:total sequences 2356, passing sequences 2356, failing sequences 0
INFO(140086093551360)2019-08-09 15:39:26,422:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/253-71-10_43_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:39:39,342:deblurring 2356 sequences
INFO(140086093551360)2019-08-09 15:39:44,185:779 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:39:44,192:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/253-71-10_43_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:39:45,304:finished processing file
INFO(140086093551360)2019-08-09 15:39:45,306:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:39:45,306:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/416-36-10_69_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:39:58,832:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/416-36-10_69_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:39:58,894:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/416-36-10_69_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:40:00,692:total sequences 1464, passing sequences 1464, failing sequences 0
INFO(140086093551360)2019-08-09 15:40:00,693:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/416-36-10_69_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:40:06,835:deblurring 1464 sequences
INFO(140086093551360)2019-08-09 15:40:09,178:607 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:40:09,185:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/416-36-10_69_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:40:09,989:finished processing file
INFO(140086093551360)2019-08-09 15:40:09,990:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:40:09,991:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/419-32-62_87_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:40:44,598:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-32-62_87_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:40:44,783:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-32-62_87_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:40:46,918:total sequences 3497, passing sequences 3497, failing sequences 0
INFO(140086093551360)2019-08-09 15:40:46,918:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-32-62_87_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:41:12,708:deblurring 3497 sequences
INFO(140086093551360)2019-08-09 15:41:20,652:871 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:41:20,664:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-32-62_87_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:41:21,916:finished processing file
INFO(140086093551360)2019-08-09 15:41:21,919:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:41:21,919:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/050-21-99_4_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:41:38,721:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/050-21-99_4_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:41:38,793:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/050-21-99_4_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:41:40,638:total sequences 1758, passing sequences 1758, failing sequences 0
INFO(140086093551360)2019-08-09 15:41:40,638:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/050-21-99_4_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:41:48,809:deblurring 1758 sequences
INFO(140086093551360)2019-08-09 15:41:52,277:695 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:41:52,286:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/050-21-99_4_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:41:53,249:finished processing file
INFO(140086093551360)2019-08-09 15:41:53,250:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:41:53,251:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/420-25-00_97_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:42:25,917:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-25-00_97_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:42:26,057:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-25-00_97_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:42:28,223:total sequences 3808, passing sequences 3808, failing sequences 0
INFO(140086093551360)2019-08-09 15:42:28,223:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-25-00_97_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:43:00,740:deblurring 3808 sequences
INFO(140086093551360)2019-08-09 15:43:05,472:404 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:43:05,478:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/420-25-00_97_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:43:06,010:finished processing file
INFO(140086093551360)2019-08-09 15:43:06,013:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:43:06,013:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/182-28-22_19_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:43:33,317:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/182-28-22_19_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:43:33,796:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/182-28-22_19_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:43:35,839:total sequences 2807, passing sequences 2807, failing sequences 0
INFO(140086093551360)2019-08-09 15:43:35,839:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/182-28-22_19_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:43:53,397:deblurring 2807 sequences
INFO(140086093551360)2019-08-09 15:43:59,576:852 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:43:59,587:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/182-28-22_19_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:44:00,748:finished processing file
INFO(140086093551360)2019-08-09 15:44:00,751:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:44:00,751:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/559-67-83_106_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:44:38,580:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/559-67-83_106_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:44:38,750:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/559-67-83_106_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:44:41,029:total sequences 4052, passing sequences 4052, failing sequences 0
INFO(140086093551360)2019-08-09 15:44:41,029:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/559-67-83_106_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:45:16,562:deblurring 4052 sequences
INFO(140086093551360)2019-08-09 15:45:34,577:1528 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:45:34,626:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/559-67-83_106_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:45:36,851:finished processing file
INFO(140086093551360)2019-08-09 15:45:36,855:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:45:36,855:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/421-62-50_105_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:45:51,923:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-62-50_105_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:45:52,024:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-62-50_105_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:45:54,261:total sequences 1535, passing sequences 1535, failing sequences 0
INFO(140086093551360)2019-08-09 15:45:54,262:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-62-50_105_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:46:01,038:deblurring 1535 sequences
INFO(140086093551360)2019-08-09 15:46:03,057:507 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:46:03,063:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/421-62-50_105_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:46:03,705:finished processing file
INFO(140086093551360)2019-08-09 15:46:03,707:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:46:03,707:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/068_71_13_1_6_L001_R1_001.fastq.gz
WARNING(140086093551360)2019-08-09 15:46:38,634:Vast majority of sequences (62560 / 62560) are shorter than the trim length (280). Are you using the correct -t trim length?
INFO(140086093551360)2019-08-09 15:46:38,635:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/068_71_13_1_6_L001_R1_001.fastq.gz.trim
ERROR(140086093551360)2019-08-09 15:46:38,648:Problem running vsearch dereplication on file /tmp/tmpefmj40f_/deblur_working_dir/068_71_13_1_6_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:46:38,648:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/068_71_13_1_6_L001_R1_001.fastq.gz.trim.derep
WARNING(140086093551360)2019-08-09 15:46:38,648:file /tmp/tmpefmj40f_/deblur_working_dir/068_71_13_1_6_L001_R1_001.fastq.gz.trim.derep has size 0, continuing
WARNING(140086093551360)2019-08-09 15:46:38,648:remove artifacts failed, aborting
WARNING(140086093551360)2019-08-09 15:46:38,648:deblurring failed for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/068_71_13_1_6_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:46:38,649:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:46:38,649:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/288-32-30_51_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:47:31,106:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/288-32-30_51_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:47:35,847:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/288-32-30_51_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:47:42,174:total sequences 4581, passing sequences 4581, failing sequences 0
INFO(140086093551360)2019-08-09 15:47:42,174:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/288-32-30_51_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:48:42,486:deblurring 4581 sequences
INFO(140086093551360)2019-08-09 15:48:56,150:1122 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:48:56,168:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/288-32-30_51_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:48:57,835:finished processing file
INFO(140086093551360)2019-08-09 15:48:57,871:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:48:57,871:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/268-35-64_49_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:49:21,407:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/268-35-64_49_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:49:21,531:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/268-35-64_49_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:49:23,501:total sequences 1958, passing sequences 1958, failing sequences 0
INFO(140086093551360)2019-08-09 15:49:23,501:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/268-35-64_49_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:49:33,013:deblurring 1958 sequences
INFO(140086093551360)2019-08-09 15:49:36,863:771 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:49:36,871:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/268-35-64_49_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:49:37,936:finished processing file
INFO(140086093551360)2019-08-09 15:49:37,938:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:49:37,938:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/221-90-44_36_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:49:54,989:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/221-90-44_36_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:49:55,062:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/221-90-44_36_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:49:56,916:total sequences 1798, passing sequences 1798, failing sequences 0
INFO(140086093551360)2019-08-09 15:49:56,916:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/221-90-44_36_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:50:05,456:deblurring 1798 sequences
INFO(140086093551360)2019-08-09 15:50:09,281:862 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:50:09,292:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/221-90-44_36_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:50:10,509:finished processing file
INFO(140086093551360)2019-08-09 15:50:10,511:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:50:10,511:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/410_27_19_1_64_L001_R1_001.fastq.gz
WARNING(140086093551360)2019-08-09 15:51:06,427:Vast majority of sequences (61620 / 61620) are shorter than the trim length (280). Are you using the correct -t trim length?
INFO(140086093551360)2019-08-09 15:51:07,302:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_27_19_1_64_L001_R1_001.fastq.gz.trim
ERROR(140086093551360)2019-08-09 15:51:13,792:Problem running vsearch dereplication on file /tmp/tmpefmj40f_/deblur_working_dir/410_27_19_1_64_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:51:13,836:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_27_19_1_64_L001_R1_001.fastq.gz.trim.derep
WARNING(140086093551360)2019-08-09 15:51:13,872:file /tmp/tmpefmj40f_/deblur_working_dir/410_27_19_1_64_L001_R1_001.fastq.gz.trim.derep has size 0, continuing
WARNING(140086093551360)2019-08-09 15:51:13,876:remove artifacts failed, aborting
WARNING(140086093551360)2019-08-09 15:51:13,885:deblurring failed for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/410_27_19_1_64_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:51:13,885:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:51:13,885:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/419-01-94_84_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:51:45,606:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-01-94_84_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:51:46,369:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-01-94_84_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:52:00,992:total sequences 2562, passing sequences 2562, failing sequences 0
INFO(140086093551360)2019-08-09 15:52:00,992:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-01-94_84_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:52:33,261:deblurring 2562 sequences
INFO(140086093551360)2019-08-09 15:52:39,735:1012 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:52:39,747:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-01-94_84_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:52:41,211:finished processing file
INFO(140086093551360)2019-08-09 15:52:41,214:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:52:41,214:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/087-24-42_8_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:53:41,058:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/087-24-42_8_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:53:48,250:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/087-24-42_8_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:53:52,310:total sequences 6086, passing sequences 6086, failing sequences 0
INFO(140086093551360)2019-08-09 15:53:52,310:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/087-24-42_8_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:57:34,248:deblurring 6086 sequences
INFO(140086093551360)2019-08-09 15:57:57,957:1630 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:57:57,977:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/087-24-42_8_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:58:00,287:finished processing file
INFO(140086093551360)2019-08-09 15:58:00,291:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:58:00,291:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/411_58_77_1_68_L001_R1_001.fastq.gz
WARNING(140086093551360)2019-08-09 15:58:33,617:Vast majority of sequences (62400 / 62400) are shorter than the trim length (280). Are you using the correct -t trim length?
INFO(140086093551360)2019-08-09 15:58:33,618:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/411_58_77_1_68_L001_R1_001.fastq.gz.trim
ERROR(140086093551360)2019-08-09 15:58:33,626:Problem running vsearch dereplication on file /tmp/tmpefmj40f_/deblur_working_dir/411_58_77_1_68_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:58:33,626:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/411_58_77_1_68_L001_R1_001.fastq.gz.trim.derep
WARNING(140086093551360)2019-08-09 15:58:33,627:file /tmp/tmpefmj40f_/deblur_working_dir/411_58_77_1_68_L001_R1_001.fastq.gz.trim.derep has size 0, continuing
WARNING(140086093551360)2019-08-09 15:58:33,627:remove artifacts failed, aborting
WARNING(140086093551360)2019-08-09 15:58:33,627:deblurring failed for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/411_58_77_1_68_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:58:33,627:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:58:33,627:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/185-06-45_25_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:58:52,128:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/185-06-45_25_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:58:52,224:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/185-06-45_25_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:58:54,641:total sequences 1866, passing sequences 1866, failing sequences 0
INFO(140086093551360)2019-08-09 15:58:54,641:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/185-06-45_25_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 15:59:03,604:deblurring 1866 sequences
INFO(140086093551360)2019-08-09 15:59:05,076:227 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 15:59:05,079:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/185-06-45_25_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 15:59:05,371:finished processing file
INFO(140086093551360)2019-08-09 15:59:05,372:--------------------------------------------------------
INFO(140086093551360)2019-08-09 15:59:05,372:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/202-70-17_28_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 15:59:40,004:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/202-70-17_28_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 15:59:40,160:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/202-70-17_28_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 15:59:42,326:total sequences 3677, passing sequences 3677, failing sequences 0
INFO(140086093551360)2019-08-09 15:59:42,326:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/202-70-17_28_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 16:00:15,805:deblurring 3677 sequences
INFO(140086093551360)2019-08-09 16:00:26,253:1164 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 16:00:26,269:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/202-70-17_28_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 16:00:27,902:finished processing file
INFO(140086093551360)2019-08-09 16:00:27,906:--------------------------------------------------------
INFO(140086093551360)2019-08-09 16:00:27,906:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/410_34_80_1_65_L001_R1_001.fastq.gz
WARNING(140086093551360)2019-08-09 16:01:01,440:Vast majority of sequences (61860 / 61860) are shorter than the trim length (280). Are you using the correct -t trim length?
INFO(140086093551360)2019-08-09 16:01:01,441:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_34_80_1_65_L001_R1_001.fastq.gz.trim
ERROR(140086093551360)2019-08-09 16:01:01,448:Problem running vsearch dereplication on file /tmp/tmpefmj40f_/deblur_working_dir/410_34_80_1_65_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 16:01:01,448:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_34_80_1_65_L001_R1_001.fastq.gz.trim.derep
WARNING(140086093551360)2019-08-09 16:01:01,448:file /tmp/tmpefmj40f_/deblur_working_dir/410_34_80_1_65_L001_R1_001.fastq.gz.trim.derep has size 0, continuing
WARNING(140086093551360)2019-08-09 16:01:01,449:remove artifacts failed, aborting
WARNING(140086093551360)2019-08-09 16:01:01,449:deblurring failed for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/410_34_80_1_65_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 16:01:01,449:--------------------------------------------------------
INFO(140086093551360)2019-08-09 16:01:01,449:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/419-93-34_91_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 16:01:34,868:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-93-34_91_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 16:01:35,016:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-93-34_91_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 16:01:37,126:total sequences 3404, passing sequences 3404, failing sequences 0
INFO(140086093551360)2019-08-09 16:01:37,126:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-93-34_91_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 16:02:06,012:deblurring 3404 sequences
INFO(140086093551360)2019-08-09 16:02:16,976:1320 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 16:02:16,992:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-93-34_91_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 16:02:18,831:finished processing file
INFO(140086093551360)2019-08-09 16:02:18,834:--------------------------------------------------------
INFO(140086093551360)2019-08-09 16:02:18,834:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/135-78-82_13_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 16:02:36,899:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/135-78-82_13_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 16:02:36,990:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/135-78-82_13_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 16:02:38,914:total sequences 2119, passing sequences 2119, failing sequences 0
INFO(140086093551360)2019-08-09 16:02:38,914:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/135-78-82_13_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 16:02:49,784:deblurring 2119 sequences
INFO(140086093551360)2019-08-09 16:02:54,115:809 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 16:02:54,123:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/135-78-82_13_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 16:02:55,221:finished processing file
INFO(140086093551360)2019-08-09 16:02:55,223:--------------------------------------------------------
INFO(140086093551360)2019-08-09 16:02:55,223:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/221-39-18_35_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 16:03:22,878:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/221-39-18_35_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 16:03:23,005:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/221-39-18_35_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 16:03:25,113:total sequences 3295, passing sequences 3295, failing sequences 0
INFO(140086093551360)2019-08-09 16:03:25,114:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/221-39-18_35_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 16:03:49,147:deblurring 3295 sequences
INFO(140086093551360)2019-08-09 16:03:53,836:485 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 16:03:53,843:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/221-39-18_35_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 16:03:54,482:finished processing file
INFO(140086093551360)2019-08-09 16:03:54,484:--------------------------------------------------------
INFO(140086093551360)2019-08-09 16:03:54,484:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/405-76-49_58_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 16:04:04,421:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/405-76-49_58_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 16:04:04,466:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/405-76-49_58_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 16:04:06,216:total sequences 1047, passing sequences 1047, failing sequences 0
INFO(140086093551360)2019-08-09 16:04:06,216:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/405-76-49_58_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 16:04:10,023:deblurring 1047 sequences
INFO(140086093551360)2019-08-09 16:04:11,338:468 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 16:04:11,343:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/405-76-49_58_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 16:04:11,955:finished processing file
INFO(140086093551360)2019-08-09 16:04:11,956:--------------------------------------------------------
INFO(140086093551360)2019-08-09 16:04:11,956:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/231-50-35_38_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 16:05:07,593:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/231-50-35_38_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 16:05:07,859:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/231-50-35_38_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 16:05:10,365:total sequences 5809, passing sequences 5809, failing sequences 0
INFO(140086093551360)2019-08-09 16:05:10,365:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/231-50-35_38_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 16:07:17,247:deblurring 5809 sequences
INFO(140086093551360)2019-08-09 16:07:35,618:1282 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 16:07:35,681:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/231-50-35_38_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 16:07:37,449:finished processing file
INFO(140086093551360)2019-08-09 16:07:37,453:--------------------------------------------------------
INFO(140086093551360)2019-08-09 16:07:37,453:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/263-52-54_45_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 16:08:00,794:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/263-52-54_45_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 16:08:00,890:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/263-52-54_45_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 16:08:02,996:total sequences 2243, passing sequences 2243, failing sequences 0
INFO(140086093551360)2019-08-09 16:08:02,996:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/263-52-54_45_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 16:08:15,201:deblurring 2243 sequences
INFO(140086093551360)2019-08-09 16:08:19,101:695 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 16:08:19,110:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/263-52-54_45_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 16:08:20,007:finished processing file
INFO(140086093551360)2019-08-09 16:08:20,009:--------------------------------------------------------
INFO(140086093551360)2019-08-09 16:08:20,010:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/266-09-18_47_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 16:08:34,428:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/266-09-18_47_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 16:08:34,495:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/266-09-18_47_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 16:08:36,309:total sequences 1477, passing sequences 1477, failing sequences 0
INFO(140086093551360)2019-08-09 16:08:36,309:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/266-09-18_47_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 16:08:42,609:deblurring 1477 sequences
INFO(140086093551360)2019-08-09 16:08:44,029:315 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 16:08:44,032:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/266-09-18_47_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 16:08:44,448:finished processing file
INFO(140086093551360)2019-08-09 16:08:44,449:--------------------------------------------------------
INFO(140086093551360)2019-08-09 16:08:44,449:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/410_03_84_1_61_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 16:09:07,480:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_03_84_1_61_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 16:09:07,566:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_03_84_1_61_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 16:09:09,373:total sequences 1352, passing sequences 1352, failing sequences 0
INFO(140086093551360)2019-08-09 16:09:09,373:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_03_84_1_61_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 16:09:15,690:deblurring 1352 sequences
INFO(140086093551360)2019-08-09 16:09:16,740:335 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 16:09:16,744:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/410_03_84_1_61_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 16:09:17,200:finished processing file
INFO(140086093551360)2019-08-09 16:09:17,202:--------------------------------------------------------
INFO(140086093551360)2019-08-09 16:09:17,202:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/267-75-43_48_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 16:28:59,085:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/267-75-43_48_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 16:34:24,625:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/267-75-43_48_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 16:34:41,405:total sequences 85508, passing sequences 85508, failing sequences 0
INFO(140086093551360)2019-08-09 16:34:41,405:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/267-75-43_48_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 16:59:26,350:msa failed for file /tmp/tmpefmj40f_/deblur_working_dir/267-75-43_48_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?)
WARNING(140086093551360)2019-08-09 16:59:27,044:msa failed. aborting
WARNING(140086093551360)2019-08-09 16:59:27,206:deblurring failed for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/267-75-43_48_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 16:59:27,379:--------------------------------------------------------
INFO(140086093551360)2019-08-09 16:59:27,391:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/029-34-62_2_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 16:59:44,710:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/029-34-62_2_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 16:59:45,347:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/029-34-62_2_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 16:59:47,489:total sequences 1452, passing sequences 1452, failing sequences 0
INFO(140086093551360)2019-08-09 16:59:47,489:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/029-34-62_2_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 16:59:54,669:deblurring 1452 sequences
INFO(140086093551360)2019-08-09 16:59:56,945:586 unique sequences left following deblurring
INFO(140086093551360)2019-08-09 16:59:56,971:remove_chimeras_denovo_from_seqs seqs file /tmp/tmpefmj40f_/deblur_working_dir/029-34-62_2_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmpefmj40f_/deblur_working_dir
INFO(140086093551360)2019-08-09 16:59:57,712:finished processing file
INFO(140086093551360)2019-08-09 16:59:57,714:--------------------------------------------------------
INFO(140086093551360)2019-08-09 16:59:57,714:launch_workflow for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/419-94-71_92_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 17:17:08,226:dereplicate seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-94-71_92_L001_R1_001.fastq.gz.trim
INFO(140086093551360)2019-08-09 17:17:13,796:remove_artifacts_seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-94-71_92_L001_R1_001.fastq.gz.trim.derep
INFO(140086093551360)2019-08-09 17:17:28,977:total sequences 86518, passing sequences 86518, failing sequences 0
INFO(140086093551360)2019-08-09 17:17:28,977:multiple_sequence_alignment seqs file /tmp/tmpefmj40f_/deblur_working_dir/419-94-71_92_L001_R1_001.fastq.gz.trim.derep.no_artifacts
INFO(140086093551360)2019-08-09 17:42:59,115:msa failed for file /tmp/tmpefmj40f_/deblur_working_dir/419-94-71_92_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?)
WARNING(140086093551360)2019-08-09 17:42:59,678:msa failed. aborting
WARNING(140086093551360)2019-08-09 17:42:59,761:deblurring failed for file /tmp/qiime2-archive-wy54zjkc/60fe045d-5406-4a30-8285-b7bd8359190a/data/419-94-71_92_L001_R1_001.fastq.gz
INFO(140086093551360)2019-08-09 17:42:59,778:finished processing per sample fasta files
INFO(140086093551360)2019-08-09 17:43:00,660:create_otu_table for 100 samples, into output table /tmp/tmpefmj40f_/all.biom
INFO(140086093551360)2019-08-09 17:43:20,083:for output biom table loaded 100 samples, 30651 unique sequences
INFO(140086093551360)2019-08-09 17:43:20,498:keeping 2913 (out of 30651 sequences) with >=10 reads
INFO(140086093551360)2019-08-09 17:43:26,024:saved to biom file /tmp/tmpefmj40f_/all.biom
INFO(140086093551360)2019-08-09 17:43:26,063:saved sequence fasta file to /tmp/tmpefmj40f_/all.seqs.fa
INFO(140086093551360)2019-08-09 17:43:26,093:getting 16s sequences from the biom table
INFO(140086093551360)2019-08-09 17:43:26,112:remove_artifacts_seqs file /tmp/tmpefmj40f_/all.seqs.fa
INFO(140086093551360)2019-08-09 17:44:16,497:total sequences 2913, passing sequences 2912, failing sequences 1
WARNING(140086093551360)2019-08-09 17:44:16,954:removed 99 samples with reads per sample<1
INFO(140086093551360)2019-08-09 17:44:16,966:wrote artifact only filtered biom table to /tmp/tmpefmj40f_/reference-non-hit.biom
INFO(140086093551360)2019-08-09 17:44:16,966:saved biom table sequences to fasta file /tmp/tmpefmj40f_/reference-non-hit.seqs.fa
INFO(140086093551360)2019-08-09 17:44:17,015:wrote 16s filtered biom table to /tmp/tmpefmj40f_/reference-hit.biom
INFO(140086093551360)2019-08-09 17:44:17,020:saved biom table sequences to fasta file /tmp/tmpefmj40f_/reference-hit.seqs.fa
INFO(140086093551360)2019-08-09 17:44:17,034:Keeping temp files
INFO(140086093551360)2019-08-09 17:44:17,035:deblur workflow finished
INFO(140086093551360)2019-08-09 17:44:17,035:output saved to /tmp/tmpefmj40f_/all.biom
INFO(140086093551360)2019-08-09 17:44:17,035:------------------