Running external command line application(s). This may print messages to stdout and/or stderr.
The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist.

Command: run_aldex2.R /var/folders/15/3dh5kykd50n5wsnyb3d4v0040000gn/T/tmp63wfeg15/input.tsv.biom /var/folders/15/3dh5kykd50n5wsnyb3d4v0040000gn/T/tmp63wfeg15/input.map.txt type 128 t all /var/folders/15/3dh5kykd50n5wsnyb3d4v0040000gn/T/tmp63wfeg15/output.summary.txt

R version 4.0.2 (2020-06-22) 
Loading required package: zCompositions
Loading required package: MASS
Error: package or namespace load failed for ‘MASS’ in library.dynam(lib, package, package.lib):
 shared object ‘MASS.dylib’ not found
Error: package ‘MASS’ could not be loaded
Execution halted
Traceback (most recent call last):
  File "/Users/lyskae/opt/anaconda3/envs/qiime2-2020.11/lib/python3.6/site-packages/q2_aldex2/_method.py", line 59, in aldex2
    run_commands([cmd])
  File "/Users/lyskae/opt/anaconda3/envs/qiime2-2020.11/lib/python3.6/site-packages/q2_aldex2/_method.py", line 21, in run_commands
    subprocess.run(cmd, check=True)
  File "/Users/lyskae/opt/anaconda3/envs/qiime2-2020.11/lib/python3.6/subprocess.py", line 438, in run
    output=stdout, stderr=stderr)
subprocess.CalledProcessError: Command '['run_aldex2.R', '/var/folders/15/3dh5kykd50n5wsnyb3d4v0040000gn/T/tmp63wfeg15/input.tsv.biom', '/var/folders/15/3dh5kykd50n5wsnyb3d4v0040000gn/T/tmp63wfeg15/input.map.txt', 'type', '128', 't', 'all', '/var/folders/15/3dh5kykd50n5wsnyb3d4v0040000gn/T/tmp63wfeg15/output.summary.txt']' returned non-zero exit status 1.

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/lyskae/opt/anaconda3/envs/qiime2-2020.11/lib/python3.6/site-packages/q2cli/commands.py", line 329, in __call__
    results = action(**arguments)
  File "<decorator-gen-542>", line 2, in aldex2
  File "/Users/lyskae/opt/anaconda3/envs/qiime2-2020.11/lib/python3.6/site-packages/qiime2/sdk/action.py", line 245, in bound_callable
    output_types, provenance)
  File "/Users/lyskae/opt/anaconda3/envs/qiime2-2020.11/lib/python3.6/site-packages/qiime2/sdk/action.py", line 390, in _callable_executor_
    output_views = self._callable(**view_args)
  File "/Users/lyskae/opt/anaconda3/envs/qiime2-2020.11/lib/python3.6/site-packages/q2_aldex2/_method.py", line 63, in aldex2
    " and stderr to learn more." % e.returncode)
Exception: An error was encountered while running ALDEx2 in R (return code 1), please inspect stdout and stderr to learn more.