aligment map loaded
/home/andres/miniconda3/envs/qiime2/lib/python3.8/site-packages/distributed/worker.py:2845: UserWarning: Large object of size 7.10 MiB detected in task graph: 
  ('DataFrame-35f3aa34-ec98-48d3-beac-aa90cf498728', ...  dtype='<U28'))
Consider scattering large objects ahead of time
with client.scatter to reduce scheduler burden and 
keep data on workers

    future = client.submit(func, big_data)    # bad

    big_future = client.scatter(big_data)     # good
    future = client.submit(func, big_future)  # good
  warnings.warn(
2023-04-21 13:16:00,954 - distributed.worker_memory - WARNING - Worker tcp://127.0.0.1:42675 (pid=18172) exceeded 95% memory budget. Restarting...
2023-04-21 13:16:01,072 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.86 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:01,073 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.86 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:01,100 - distributed.nanny - WARNING - Restarting worker
2023-04-21 13:16:01,175 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.90 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:01,256 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:01,272 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:01,274 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.94 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:01,376 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.86 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:01,472 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.91 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:01,571 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.95 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:01,670 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.99 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:01,723 - distributed.worker_memory - WARNING - Worker tcp://127.0.0.1:40821 (pid=18184) exceeded 95% memory budget. Restarting...
2023-04-21 13:16:01,906 - distributed.nanny - WARNING - Restarting worker
2023-04-21 13:16:02,223 - distributed.worker_memory - WARNING - Worker is at 80% memory usage. Pausing worker.  Process memory: 2.04 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:02,223 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.04 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:02,249 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.05 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:02,271 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.06 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:02,387 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.13 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:02,470 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.16 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:02,572 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.19 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:02,670 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.23 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:02,770 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.28 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:02,876 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.34 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:02,970 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.39 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:03,071 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.45 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:03,104 - distributed.worker_memory - WARNING - Worker tcp://127.0.0.1:40753 (pid=18173) exceeded 95% memory budget. Restarting...
2023-04-21 13:16:03,254 - distributed.nanny - WARNING - Restarting worker
2023-04-21 13:16:05,802 - distributed.worker_memory - WARNING - Worker tcp://127.0.0.1:41827 (pid=18177) exceeded 95% memory budget. Restarting...
2023-04-21 13:16:05,937 - distributed.nanny - WARNING - Restarting worker
2023-04-21 13:16:06,837 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.81 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:06,855 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:06,954 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.87 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:07,053 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.91 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:07,154 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.96 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:07,471 - distributed.worker_memory - WARNING - Worker tcp://127.0.0.1:40003 (pid=18186) exceeded 95% memory budget. Restarting...
2023-04-21 13:16:07,621 - distributed.nanny - WARNING - Restarting worker
2023-04-21 13:16:08,764 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:08,765 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:08,835 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:08,938 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:09,035 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:09,136 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:09,235 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:09,336 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:09,438 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:09,536 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:09,636 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:09,735 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:09,835 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:09,938 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:10,036 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:10,136 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:10,235 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:10,335 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:10,439 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:10,536 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:10,686 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:10,818 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:10,820 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.82 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:10,917 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.85 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:11,016 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.88 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:11,118 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.91 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:11,221 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.97 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:11,316 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.99 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:11,716 - distributed.worker_memory - WARNING - Worker is at 80% memory usage. Pausing worker.  Process memory: 2.05 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:11,719 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.05 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:11,739 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.06 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:11,826 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.08 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:11,916 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.13 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:12,020 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.17 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:12,116 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.23 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:12,225 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.29 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:12,317 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.34 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:12,417 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.39 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:12,518 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.44 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:12,523 - distributed.worker_memory - WARNING - Worker tcp://127.0.0.1:45259 (pid=18263) exceeded 95% memory budget. Restarting...
2023-04-21 13:16:12,680 - distributed.nanny - WARNING - Restarting worker
2023-04-21 13:16:12,954 - distributed.worker_memory - WARNING - Worker tcp://127.0.0.1:45007 (pid=18255) exceeded 95% memory budget. Restarting...
2023-04-21 13:16:13,129 - distributed.nanny - WARNING - Restarting worker
2023-04-21 13:16:14,660 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.83 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:14,691 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.84 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:14,796 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.90 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:14,890 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.96 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:15,203 - distributed.worker_memory - WARNING - Worker tcp://127.0.0.1:43507 (pid=18288) exceeded 95% memory budget. Restarting...
2023-04-21 13:16:15,366 - distributed.nanny - WARNING - Restarting worker
2023-04-21 13:16:15,704 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.85 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:15,723 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.85 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:15,750 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.86 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:15,851 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 1.89 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:16,278 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.01 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:16,356 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.03 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:16,805 - distributed.worker_memory - WARNING - Worker is at 80% memory usage. Pausing worker.  Process memory: 2.05 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:16,806 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.05 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:16,808 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.05 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:16,837 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.05 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:16,967 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.05 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:17,038 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.05 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:17,137 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.05 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:17,242 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.05 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:17,344 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.05 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:17,449 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.05 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:17,538 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.09 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:17,636 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.13 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:17,735 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.18 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:17,835 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.24 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:17,944 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.30 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:18,043 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.35 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:18,135 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.40 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:18,235 - distributed.worker_memory - WARNING - Unmanaged memory use is high. This may indicate a memory leak or the memory may not be released to the OS; see https://distributed.dask.org/en/latest/worker-memory.html#memory-not-released-back-to-the-os for more information. -- Unmanaged memory: 2.45 GiB -- Worker memory limit: 2.55 GiB
2023-04-21 13:16:18,272 - distributed.worker_memory - WARNING - Worker tcp://127.0.0.1:39045 (pid=18181) exceeded 95% memory budget. Restarting...
2023-04-21 13:16:18,459 - distributed.nanny - WARNING - Restarting worker
2023-04-21 13:16:19,772 - distributed.worker_memory - WARNING - Worker tcp://127.0.0.1:43699 (pid=18302) exceeded 95% memory budget. Restarting...
2023-04-21 13:16:20,029 - distributed.nanny - WARNING - Restarting worker
Traceback (most recent call last):
  File "/home/andres/miniconda3/envs/qiime2/lib/python3.8/site-packages/q2cli/commands.py", line 339, in __call__
    results = action(**arguments)
  File "<decorator-gen-522>", line 2, in reconstruct_database
  File "/home/andres/miniconda3/envs/qiime2/lib/python3.8/site-packages/qiime2/sdk/action.py", line 245, in bound_callable
    outputs = self._callable_executor_(scope, callable_args,
  File "/home/andres/miniconda3/envs/qiime2/lib/python3.8/site-packages/qiime2/sdk/action.py", line 391, in _callable_executor_
    output_views = self._callable(**view_args)
  File "/home/andres/miniconda3/envs/qiime2/lib/python3.8/site-packages/q2_sidle/_build_database.py", line 82, in reconstruct_database
    db_seqs = dask.compute(*[_pull_unique(df) for df in kmer_alignments])
  File "/home/andres/miniconda3/envs/qiime2/lib/python3.8/site-packages/dask/base.py", line 603, in compute
    results = schedule(dsk, keys, **kwargs)
  File "/home/andres/miniconda3/envs/qiime2/lib/python3.8/site-packages/distributed/client.py", line 3000, in get
    results = self.gather(packed, asynchronous=asynchronous, direct=direct)
  File "/home/andres/miniconda3/envs/qiime2/lib/python3.8/site-packages/distributed/client.py", line 2174, in gather
    return self.sync(
  File "/home/andres/miniconda3/envs/qiime2/lib/python3.8/site-packages/distributed/utils.py", line 338, in sync
    return sync(
  File "/home/andres/miniconda3/envs/qiime2/lib/python3.8/site-packages/distributed/utils.py", line 405, in sync
    raise exc.with_traceback(tb)
  File "/home/andres/miniconda3/envs/qiime2/lib/python3.8/site-packages/distributed/utils.py", line 378, in f
    result = yield future
  File "/home/andres/miniconda3/envs/qiime2/lib/python3.8/site-packages/tornado/gen.py", line 762, in run
    value = future.result()
  File "/home/andres/miniconda3/envs/qiime2/lib/python3.8/site-packages/distributed/client.py", line 2037, in _gather
    raise exception.with_traceback(traceback)
distributed.scheduler.KilledWorker: ('_pull_unique-bfacf729-1937-4f33-9bc8-98e8cd6da9a5', <WorkerState 'tcp://127.0.0.1:43699', name: 1, status: closed, memory: 0, processing: 1>)