INFO(139958420399936)2022-06-30 08:58:05,867:************************* INFO(139958420399936)2022-06-30 08:58:05,867:deblurring started WARNING(139958420399936)2022-06-30 08:58:05,867:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-o2kqvhck/8fbef049-d032-4b6b-9175-29c7309e2b58/data WARNING(139958420399936)2022-06-30 08:58:05,867:parameters: {'seqs_fp': '/tmp/qiime2-archive-o2kqvhck/8fbef049-d032-4b6b-9175-29c7309e2b58/data', 'output_dir': '/tmp/tmp1of4dpa9', 'pos_ref_fp': (), 'pos_ref_db_fp': (), 'neg_ref_fp': (), 'neg_ref_db_fp': (), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': False, 'logger': } INFO(139958420399936)2022-06-30 08:58:05,867:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139958420399936)2022-06-30 08:58:05,867:deblur main program started INFO(139958420399936)2022-06-30 08:58:05,867:processing directory /tmp/qiime2-archive-o2kqvhck/8fbef049-d032-4b6b-9175-29c7309e2b58/data INFO(139958420399936)2022-06-30 08:58:05,868:building negative db sortmerna index files INFO(139958420399936)2022-06-30 08:58:05,868:build_index_sortmerna files ['/home/ubuntu/Software/miniconda3/envs/qiime2-2022.2/lib/python3.8/site-packages/deblur/support_files/artifacts.fa'] to dir /tmp/tmp1of4dpa9/deblur_working_dir INFO(139958420399936)2022-06-30 08:58:06,899:building positive db sortmerna index files INFO(139958420399936)2022-06-30 08:58:06,899:build_index_sortmerna files ['/home/ubuntu/Software/miniconda3/envs/qiime2-2022.2/lib/python3.8/site-packages/deblur/support_files/88_otus.fasta'] to dir /tmp/tmp1of4dpa9/deblur_working_dir INFO(139958420399936)2022-06-30 08:58:39,835:processing per sample fasta files INFO(139958420399936)2022-06-30 08:58:39,835:-------------------------------------------------------- INFO(139958420399936)2022-06-30 08:58:39,835:launch_workflow for file /tmp/qiime2-archive-o2kqvhck/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-11_10_L001_R1_001.fastq.gz INFO(139958420399936)2022-06-30 09:47:02,138:dereplicate seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim INFO(139958420399936)2022-06-30 09:47:26,906:remove_artifacts_seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim.derep INFO(139958420399936)2022-06-30 09:48:04,722:total sequences 1565145, passing sequences 1565145, failing sequences 0 INFO(139958420399936)2022-06-30 09:48:04,722:multiple_sequence_alignment seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139958420399936)2022-06-30 15:39:42,907:msa failed for file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139958420399936)2022-06-30 15:39:45,518:msa failed. aborting WARNING(139958420399936)2022-06-30 15:39:45,518:deblurring failed for file /tmp/qiime2-archive-o2kqvhck/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-11_10_L001_R1_001.fastq.gz INFO(139958420399936)2022-06-30 15:39:46,131:-------------------------------------------------------- INFO(139958420399936)2022-06-30 15:39:46,131:launch_workflow for file /tmp/qiime2-archive-o2kqvhck/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-33_32_L001_R1_001.fastq.gz INFO(139958420399936)2022-06-30 16:30:44,669:dereplicate seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim INFO(139958420399936)2022-06-30 16:31:12,192:remove_artifacts_seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim.derep INFO(139958420399936)2022-06-30 16:31:59,212:total sequences 1820594, passing sequences 1820594, failing sequences 0 INFO(139958420399936)2022-06-30 16:31:59,212:multiple_sequence_alignment seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139958420399936)2022-06-30 23:20:55,626:msa failed for file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139958420399936)2022-06-30 23:20:56,827:msa failed. aborting WARNING(139958420399936)2022-06-30 23:20:56,827:deblurring failed for file /tmp/qiime2-archive-o2kqvhck/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-33_32_L001_R1_001.fastq.gz INFO(139958420399936)2022-06-30 23:20:57,258:-------------------------------------------------------- INFO(139958420399936)2022-06-30 23:20:57,258:launch_workflow for file /tmp/qiime2-archive-o2kqvhck/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-13_12_L001_R1_001.fastq.gz INFO(139958420399936)2022-07-01 00:06:16,424:dereplicate seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim INFO(139958420399936)2022-07-01 00:06:40,337:remove_artifacts_seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim.derep INFO(139958420399936)2022-07-01 00:07:16,362:total sequences 1509786, passing sequences 1509786, failing sequences 0 INFO(139958420399936)2022-07-01 00:07:16,362:multiple_sequence_alignment seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139958420399936)2022-07-01 05:31:30,672:msa failed for file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139958420399936)2022-07-01 05:31:30,734:msa failed. aborting WARNING(139958420399936)2022-07-01 05:31:30,734:deblurring failed for file /tmp/qiime2-archive-o2kqvhck/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-13_12_L001_R1_001.fastq.gz INFO(139958420399936)2022-07-01 05:31:30,856:-------------------------------------------------------- INFO(139958420399936)2022-07-01 05:31:30,856:launch_workflow for file /tmp/qiime2-archive-o2kqvhck/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-09_8_L001_R1_001.fastq.gz INFO(139958420399936)2022-07-01 06:12:03,360:dereplicate seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim INFO(139958420399936)2022-07-01 06:12:22,059:remove_artifacts_seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim.derep INFO(139958420399936)2022-07-01 06:12:54,949:total sequences 1383381, passing sequences 1383381, failing sequences 0 INFO(139958420399936)2022-07-01 06:12:54,949:multiple_sequence_alignment seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139958420399936)2022-07-01 11:22:37,030:msa failed for file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139958420399936)2022-07-01 11:22:37,323:msa failed. aborting WARNING(139958420399936)2022-07-01 11:22:37,324:deblurring failed for file /tmp/qiime2-archive-o2kqvhck/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-09_8_L001_R1_001.fastq.gz INFO(139958420399936)2022-07-01 11:22:37,590:-------------------------------------------------------- INFO(139958420399936)2022-07-01 11:22:37,590:launch_workflow for file /tmp/qiime2-archive-o2kqvhck/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-31_30_L001_R1_001.fastq.gz INFO(139958420399936)2022-07-01 12:15:25,085:dereplicate seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim INFO(139958420399936)2022-07-01 12:16:03,737:remove_artifacts_seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim.derep INFO(139958420399936)2022-07-01 12:16:52,965:total sequences 1271888, passing sequences 1271888, failing sequences 0 INFO(139958420399936)2022-07-01 12:16:52,965:multiple_sequence_alignment seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139958420399936)2022-07-01 17:17:33,432:msa failed for file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139958420399936)2022-07-01 17:17:34,648:msa failed. aborting WARNING(139958420399936)2022-07-01 17:17:34,648:deblurring failed for file /tmp/qiime2-archive-o2kqvhck/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-31_30_L001_R1_001.fastq.gz INFO(139958420399936)2022-07-01 17:17:35,141:-------------------------------------------------------- INFO(139958420399936)2022-07-01 17:17:35,141:launch_workflow for file /tmp/qiime2-archive-o2kqvhck/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-01_0_L001_R1_001.fastq.gz INFO(139958420399936)2022-07-01 17:59:24,495:dereplicate seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim INFO(139958420399936)2022-07-01 17:59:46,670:remove_artifacts_seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim.derep INFO(139958420399936)2022-07-01 18:00:14,994:total sequences 1193992, passing sequences 1193992, failing sequences 0 INFO(139958420399936)2022-07-01 18:00:14,994:multiple_sequence_alignment seqs file /tmp/tmp1of4dpa9/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139958420399936)2022-07-01 23:50:59,593:deblurring 1193992 sequences INFO(139792277886784)2022-07-06 10:58:58,405:************************* INFO(139792277886784)2022-07-06 10:58:58,430:deblurring started WARNING(139792277886784)2022-07-06 10:58:58,430:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data WARNING(139792277886784)2022-07-06 10:58:58,430:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': (), 'neg_ref_fp': (), 'neg_ref_db_fp': (), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 10, 'is_worker_thread': False, 'logger': } INFO(139792277886784)2022-07-06 10:58:58,430:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139792277886784)2022-07-06 10:58:58,430:deblur main program started INFO(139792277886784)2022-07-06 10:58:58,430:processing directory /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data INFO(139792277886784)2022-07-06 10:58:58,431:building negative db sortmerna index files INFO(139792277886784)2022-07-06 10:58:58,431:build_index_sortmerna files ['/home/ubuntu/Software/miniconda3/envs/qiime2-2022.2/lib/python3.8/site-packages/deblur/support_files/artifacts.fa'] to dir /tmp/tmpns9tipf_/deblur_working_dir INFO(139792277886784)2022-07-06 10:58:58,500:building positive db sortmerna index files INFO(139792277886784)2022-07-06 10:58:58,500:build_index_sortmerna files ['/home/ubuntu/Software/miniconda3/envs/qiime2-2022.2/lib/python3.8/site-packages/deblur/support_files/88_otus.fasta'] to dir /tmp/tmpns9tipf_/deblur_working_dir INFO(139792277886784)2022-07-06 10:59:28,051:parallel processing per sample fasta files INFO(139792277886784)2022-07-06 10:59:28,051:parallel deblur started for 36 inputs INFO(140010671400768)2022-07-06 10:59:35,771:************************* INFO(139918081791808)2022-07-06 10:59:35,771:************************* INFO(140010671400768)2022-07-06 10:59:35,771:deblurring started INFO(139918081791808)2022-07-06 10:59:35,771:deblurring started WARNING(140010671400768)2022-07-06 10:59:35,771:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-09_8_L001_R1_001.fastq.gz WARNING(139918081791808)2022-07-06 10:59:35,771:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-33_32_L001_R1_001.fastq.gz WARNING(140010671400768)2022-07-06 10:59:35,771:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-09_8_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } WARNING(139918081791808)2022-07-06 10:59:35,771:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-33_32_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140010671400768)2022-07-06 10:59:35,771:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139918081791808)2022-07-06 10:59:35,771:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140010671400768)2022-07-06 10:59:35,772:deblur worker thread started INFO(139918081791808)2022-07-06 10:59:35,772:deblur worker thread started INFO(140010671400768)2022-07-06 10:59:35,772:processing per sample fasta files INFO(139918081791808)2022-07-06 10:59:35,772:processing per sample fasta files INFO(140010671400768)2022-07-06 10:59:35,772:-------------------------------------------------------- INFO(139918081791808)2022-07-06 10:59:35,772:-------------------------------------------------------- INFO(140010671400768)2022-07-06 10:59:35,772:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-09_8_L001_R1_001.fastq.gz INFO(139918081791808)2022-07-06 10:59:35,772:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-33_32_L001_R1_001.fastq.gz INFO(140603917907776)2022-07-06 10:59:35,772:************************* INFO(140065511339840)2022-07-06 10:59:35,772:************************* INFO(140426519250752)2022-07-06 10:59:35,772:************************* INFO(140603917907776)2022-07-06 10:59:35,772:deblurring started INFO(140272526374720)2022-07-06 10:59:35,772:************************* INFO(140597540349760)2022-07-06 10:59:35,772:************************* INFO(140463565297472)2022-07-06 10:59:35,772:************************* INFO(140426519250752)2022-07-06 10:59:35,772:deblurring started INFO(140065511339840)2022-07-06 10:59:35,772:deblurring started INFO(140098394359616)2022-07-06 10:59:35,772:************************* INFO(139798669473600)2022-07-06 10:59:35,772:************************* INFO(140272526374720)2022-07-06 10:59:35,772:deblurring started INFO(140597540349760)2022-07-06 10:59:35,772:deblurring started INFO(140463565297472)2022-07-06 10:59:35,772:deblurring started INFO(140098394359616)2022-07-06 10:59:35,772:deblurring started INFO(139798669473600)2022-07-06 10:59:35,772:deblurring started WARNING(140603917907776)2022-07-06 10:59:35,773:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-13_12_L001_R1_001.fastq.gz WARNING(140065511339840)2022-07-06 10:59:35,773:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-01_0_L001_R1_001.fastq.gz WARNING(140426519250752)2022-07-06 10:59:35,773:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-35_34_L001_R1_001.fastq.gz WARNING(140597540349760)2022-07-06 10:59:35,773:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-34_33_L001_R1_001.fastq.gz WARNING(140272526374720)2022-07-06 10:59:35,773:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-04_3_L001_R1_001.fastq.gz WARNING(140463565297472)2022-07-06 10:59:35,773:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-31_30_L001_R1_001.fastq.gz WARNING(140603917907776)2022-07-06 10:59:35,773:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-13_12_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } WARNING(140098394359616)2022-07-06 10:59:35,773:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-11_10_L001_R1_001.fastq.gz WARNING(139798669473600)2022-07-06 10:59:35,773:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-24_23_L001_R1_001.fastq.gz WARNING(140065511339840)2022-07-06 10:59:35,773:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-01_0_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } WARNING(140426519250752)2022-07-06 10:59:35,773:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-35_34_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140603917907776)2022-07-06 10:59:35,773:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] WARNING(140272526374720)2022-07-06 10:59:35,773:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-04_3_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } WARNING(140597540349760)2022-07-06 10:59:35,773:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-34_33_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } WARNING(140463565297472)2022-07-06 10:59:35,773:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-31_30_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140065511339840)2022-07-06 10:59:35,773:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140426519250752)2022-07-06 10:59:35,773:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140603917907776)2022-07-06 10:59:35,773:deblur worker thread started WARNING(140098394359616)2022-07-06 10:59:35,773:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-11_10_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } WARNING(139798669473600)2022-07-06 10:59:35,773:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-24_23_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140272526374720)2022-07-06 10:59:35,773:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140597540349760)2022-07-06 10:59:35,773:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140426519250752)2022-07-06 10:59:35,773:deblur worker thread started INFO(140065511339840)2022-07-06 10:59:35,773:deblur worker thread started INFO(140463565297472)2022-07-06 10:59:35,773:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140603917907776)2022-07-06 10:59:35,773:processing per sample fasta files INFO(139798669473600)2022-07-06 10:59:35,773:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140098394359616)2022-07-06 10:59:35,773:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140597540349760)2022-07-06 10:59:35,773:deblur worker thread started INFO(140272526374720)2022-07-06 10:59:35,773:deblur worker thread started INFO(140065511339840)2022-07-06 10:59:35,773:processing per sample fasta files INFO(140426519250752)2022-07-06 10:59:35,773:processing per sample fasta files INFO(140463565297472)2022-07-06 10:59:35,773:deblur worker thread started INFO(140603917907776)2022-07-06 10:59:35,773:-------------------------------------------------------- INFO(140272526374720)2022-07-06 10:59:35,773:processing per sample fasta files INFO(140597540349760)2022-07-06 10:59:35,773:processing per sample fasta files INFO(139798669473600)2022-07-06 10:59:35,773:deblur worker thread started INFO(140098394359616)2022-07-06 10:59:35,773:deblur worker thread started INFO(140426519250752)2022-07-06 10:59:35,773:-------------------------------------------------------- INFO(140065511339840)2022-07-06 10:59:35,773:-------------------------------------------------------- INFO(140603917907776)2022-07-06 10:59:35,773:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-13_12_L001_R1_001.fastq.gz INFO(140463565297472)2022-07-06 10:59:35,773:processing per sample fasta files INFO(139798669473600)2022-07-06 10:59:35,773:processing per sample fasta files INFO(140098394359616)2022-07-06 10:59:35,773:processing per sample fasta files INFO(140272526374720)2022-07-06 10:59:35,773:-------------------------------------------------------- INFO(140597540349760)2022-07-06 10:59:35,773:-------------------------------------------------------- INFO(140065511339840)2022-07-06 10:59:35,773:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-01_0_L001_R1_001.fastq.gz INFO(140426519250752)2022-07-06 10:59:35,773:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-35_34_L001_R1_001.fastq.gz INFO(140463565297472)2022-07-06 10:59:35,773:-------------------------------------------------------- INFO(140272526374720)2022-07-06 10:59:35,773:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-04_3_L001_R1_001.fastq.gz INFO(140597540349760)2022-07-06 10:59:35,773:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-34_33_L001_R1_001.fastq.gz INFO(140098394359616)2022-07-06 10:59:35,773:-------------------------------------------------------- INFO(139798669473600)2022-07-06 10:59:35,773:-------------------------------------------------------- INFO(140463565297472)2022-07-06 10:59:35,773:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-31_30_L001_R1_001.fastq.gz INFO(139798669473600)2022-07-06 10:59:35,773:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-24_23_L001_R1_001.fastq.gz INFO(140098394359616)2022-07-06 10:59:35,773:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-11_10_L001_R1_001.fastq.gz INFO(140597540349760)2022-07-06 11:36:53,458:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-34_33_L001_R1_001.fastq.gz.trim INFO(140597540349760)2022-07-06 11:37:08,183:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-34_33_L001_R1_001.fastq.gz.trim.derep INFO(140597540349760)2022-07-06 11:37:33,064:total sequences 622887, passing sequences 622887, failing sequences 0 INFO(140597540349760)2022-07-06 11:37:33,064:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-34_33_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140272526374720)2022-07-06 11:47:56,160:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-04_3_L001_R1_001.fastq.gz.trim INFO(140272526374720)2022-07-06 11:48:14,777:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-04_3_L001_R1_001.fastq.gz.trim.derep INFO(140272526374720)2022-07-06 11:48:57,943:total sequences 1001392, passing sequences 1001391, failing sequences 1 INFO(140272526374720)2022-07-06 11:48:57,943:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-04_3_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139798669473600)2022-07-06 11:54:43,613:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-24_23_L001_R1_001.fastq.gz.trim INFO(139798669473600)2022-07-06 11:55:03,644:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-24_23_L001_R1_001.fastq.gz.trim.derep INFO(139798669473600)2022-07-06 11:55:50,659:total sequences 1207179, passing sequences 1207179, failing sequences 0 INFO(139798669473600)2022-07-06 11:55:50,660:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-24_23_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140010671400768)2022-07-06 11:58:54,167:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim INFO(140065511339840)2022-07-06 11:59:10,576:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim INFO(140010671400768)2022-07-06 11:59:16,313:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim.derep INFO(140065511339840)2022-07-06 11:59:44,520:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim.derep INFO(140010671400768)2022-07-06 12:00:14,957:total sequences 1383381, passing sequences 1383381, failing sequences 0 INFO(140010671400768)2022-07-06 12:00:14,958:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140065511339840)2022-07-06 12:00:28,185:total sequences 1193992, passing sequences 1193992, failing sequences 0 INFO(140065511339840)2022-07-06 12:00:28,185:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140463565297472)2022-07-06 12:01:01,146:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim INFO(140463565297472)2022-07-06 12:01:30,227:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim.derep INFO(140463565297472)2022-07-06 12:02:15,969:total sequences 1271888, passing sequences 1271888, failing sequences 0 INFO(140463565297472)2022-07-06 12:02:15,969:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140603917907776)2022-07-06 12:03:50,544:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim INFO(140603917907776)2022-07-06 12:04:27,077:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim.derep INFO(140603917907776)2022-07-06 12:05:19,009:total sequences 1509786, passing sequences 1509786, failing sequences 0 INFO(140603917907776)2022-07-06 12:05:19,009:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140098394359616)2022-07-06 12:09:18,760:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim INFO(140098394359616)2022-07-06 12:09:56,867:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim.derep INFO(140098394359616)2022-07-06 12:10:51,006:total sequences 1565145, passing sequences 1565145, failing sequences 0 INFO(140098394359616)2022-07-06 12:10:51,007:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139918081791808)2022-07-06 12:14:19,642:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim INFO(139918081791808)2022-07-06 12:14:59,070:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim.derep INFO(139918081791808)2022-07-06 12:16:00,898:total sequences 1820594, passing sequences 1820594, failing sequences 0 INFO(139918081791808)2022-07-06 12:16:00,898:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140426519250752)2022-07-06 12:19:39,351:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-35_34_L001_R1_001.fastq.gz.trim INFO(140426519250752)2022-07-06 12:20:28,445:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-35_34_L001_R1_001.fastq.gz.trim.derep INFO(140426519250752)2022-07-06 12:21:20,520:total sequences 1521846, passing sequences 1521846, failing sequences 0 INFO(140426519250752)2022-07-06 12:21:20,520:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-35_34_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140010671400768)2022-07-06 15:37:31,556:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140010671400768)2022-07-06 15:37:35,332:msa failed. aborting WARNING(140010671400768)2022-07-06 15:37:35,332:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-09_8_L001_R1_001.fastq.gz INFO(140010671400768)2022-07-06 15:37:35,332:finished processing per sample fasta files INFO(140010671400768)2022-07-06 15:37:35,332:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-09_8_L001_R1_001.fastq.gz finished INFO(140100837398336)2022-07-06 15:39:27,814:************************* INFO(140100837398336)2022-07-06 15:39:27,815:deblurring started WARNING(140100837398336)2022-07-06 15:39:27,815:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-30_29_L001_R1_001.fastq.gz WARNING(140100837398336)2022-07-06 15:39:27,815:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-30_29_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140100837398336)2022-07-06 15:39:27,815:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140100837398336)2022-07-06 15:39:27,815:deblur worker thread started INFO(140100837398336)2022-07-06 15:39:27,815:processing per sample fasta files INFO(140100837398336)2022-07-06 15:39:27,815:-------------------------------------------------------- INFO(140100837398336)2022-07-06 15:39:27,815:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-30_29_L001_R1_001.fastq.gz INFO(140463565297472)2022-07-06 15:51:09,641:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140463565297472)2022-07-06 15:51:11,316:msa failed. aborting WARNING(140463565297472)2022-07-06 15:51:11,352:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-31_30_L001_R1_001.fastq.gz INFO(140463565297472)2022-07-06 15:51:11,352:finished processing per sample fasta files INFO(140463565297472)2022-07-06 15:51:11,352:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-31_30_L001_R1_001.fastq.gz finished INFO(140119843374912)2022-07-06 15:52:22,609:************************* INFO(140119843374912)2022-07-06 15:52:22,609:deblurring started WARNING(140119843374912)2022-07-06 15:52:22,609:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-28_27_L001_R1_001.fastq.gz WARNING(140119843374912)2022-07-06 15:52:22,609:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-28_27_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140119843374912)2022-07-06 15:52:22,610:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140119843374912)2022-07-06 15:52:22,610:deblur worker thread started INFO(140119843374912)2022-07-06 15:52:22,610:processing per sample fasta files INFO(140119843374912)2022-07-06 15:52:22,610:-------------------------------------------------------- INFO(140119843374912)2022-07-06 15:52:22,610:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-28_27_L001_R1_001.fastq.gz INFO(140597540349760)2022-07-06 16:08:04,097:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-34_33_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140597540349760)2022-07-06 16:08:06,886:msa failed. aborting WARNING(140597540349760)2022-07-06 16:08:06,940:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-34_33_L001_R1_001.fastq.gz INFO(140597540349760)2022-07-06 16:08:06,940:finished processing per sample fasta files INFO(140597540349760)2022-07-06 16:08:06,940:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-34_33_L001_R1_001.fastq.gz finished INFO(139811369133888)2022-07-06 16:09:02,307:************************* INFO(139811369133888)2022-07-06 16:09:02,308:deblurring started WARNING(139811369133888)2022-07-06 16:09:02,308:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-10_9_L001_R1_001.fastq.gz WARNING(139811369133888)2022-07-06 16:09:02,308:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-10_9_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139811369133888)2022-07-06 16:09:02,308:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139811369133888)2022-07-06 16:09:02,308:deblur worker thread started INFO(139811369133888)2022-07-06 16:09:02,308:processing per sample fasta files INFO(139811369133888)2022-07-06 16:09:02,308:-------------------------------------------------------- INFO(139811369133888)2022-07-06 16:09:02,308:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-10_9_L001_R1_001.fastq.gz INFO(140065511339840)2022-07-06 16:34:43,573:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140065511339840)2022-07-06 16:34:45,139:msa failed. aborting WARNING(140065511339840)2022-07-06 16:34:45,203:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-01_0_L001_R1_001.fastq.gz INFO(140065511339840)2022-07-06 16:34:45,204:finished processing per sample fasta files INFO(140065511339840)2022-07-06 16:34:45,204:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-01_0_L001_R1_001.fastq.gz finished INFO(140119843374912)2022-07-06 16:35:06,619:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-28_27_L001_R1_001.fastq.gz.trim INFO(140401463691072)2022-07-06 16:35:58,434:************************* INFO(140401463691072)2022-07-06 16:35:58,434:deblurring started WARNING(140401463691072)2022-07-06 16:35:58,435:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-29_28_L001_R1_001.fastq.gz WARNING(140401463691072)2022-07-06 16:35:58,435:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-29_28_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140401463691072)2022-07-06 16:35:58,435:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140401463691072)2022-07-06 16:35:58,435:deblur worker thread started INFO(140401463691072)2022-07-06 16:35:58,435:processing per sample fasta files INFO(140401463691072)2022-07-06 16:35:58,435:-------------------------------------------------------- INFO(140401463691072)2022-07-06 16:35:58,435:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-29_28_L001_R1_001.fastq.gz INFO(140119843374912)2022-07-06 16:36:21,779:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-28_27_L001_R1_001.fastq.gz.trim.derep INFO(140119843374912)2022-07-06 16:36:51,997:total sequences 806843, passing sequences 806843, failing sequences 0 INFO(140119843374912)2022-07-06 16:36:51,998:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-28_27_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140100837398336)2022-07-06 16:41:59,725:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-30_29_L001_R1_001.fastq.gz.trim INFO(140100837398336)2022-07-06 16:42:45,153:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-30_29_L001_R1_001.fastq.gz.trim.derep INFO(140100837398336)2022-07-06 16:43:30,067:total sequences 1159774, passing sequences 1159774, failing sequences 0 INFO(140100837398336)2022-07-06 16:43:30,067:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-30_29_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139918081791808)2022-07-06 16:45:45,580:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139918081791808)2022-07-06 16:45:47,443:msa failed. aborting WARNING(139918081791808)2022-07-06 16:45:47,501:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-33_32_L001_R1_001.fastq.gz INFO(139918081791808)2022-07-06 16:45:47,504:finished processing per sample fasta files INFO(139918081791808)2022-07-06 16:45:47,504:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-33_32_L001_R1_001.fastq.gz finished INFO(140439722145600)2022-07-06 16:46:23,555:************************* INFO(140439722145600)2022-07-06 16:46:23,555:deblurring started WARNING(140439722145600)2022-07-06 16:46:23,555:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-23_22_L001_R1_001.fastq.gz WARNING(140439722145600)2022-07-06 16:46:23,555:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-23_22_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140439722145600)2022-07-06 16:46:23,555:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140439722145600)2022-07-06 16:46:23,555:deblur worker thread started INFO(140439722145600)2022-07-06 16:46:23,555:processing per sample fasta files INFO(140439722145600)2022-07-06 16:46:23,555:-------------------------------------------------------- INFO(140439722145600)2022-07-06 16:46:23,555:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-23_22_L001_R1_001.fastq.gz INFO(139811369133888)2022-07-06 17:03:23,309:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-10_9_L001_R1_001.fastq.gz.trim INFO(139811369133888)2022-07-06 17:04:15,490:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-10_9_L001_R1_001.fastq.gz.trim.derep INFO(139811369133888)2022-07-06 17:05:05,240:total sequences 1318258, passing sequences 1318256, failing sequences 2 INFO(139811369133888)2022-07-06 17:05:05,241:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-10_9_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140401463691072)2022-07-06 17:21:10,310:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-29_28_L001_R1_001.fastq.gz.trim INFO(140401463691072)2022-07-06 17:21:46,612:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-29_28_L001_R1_001.fastq.gz.trim.derep INFO(140401463691072)2022-07-06 17:22:36,487:total sequences 1311396, passing sequences 1311396, failing sequences 0 INFO(140401463691072)2022-07-06 17:22:36,487:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-29_28_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140603917907776)2022-07-06 17:24:08,737:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140603917907776)2022-07-06 17:24:09,816:msa failed. aborting WARNING(140603917907776)2022-07-06 17:24:09,835:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-13_12_L001_R1_001.fastq.gz INFO(140603917907776)2022-07-06 17:24:09,842:finished processing per sample fasta files INFO(140603917907776)2022-07-06 17:24:09,842:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-13_12_L001_R1_001.fastq.gz finished INFO(139945971238720)2022-07-06 17:24:43,858:************************* INFO(139945971238720)2022-07-06 17:24:43,858:deblurring started WARNING(139945971238720)2022-07-06 17:24:43,858:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-21_20_L001_R1_001.fastq.gz WARNING(139945971238720)2022-07-06 17:24:43,858:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-21_20_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139945971238720)2022-07-06 17:24:43,858:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139945971238720)2022-07-06 17:24:43,858:deblur worker thread started INFO(139945971238720)2022-07-06 17:24:43,858:processing per sample fasta files INFO(139945971238720)2022-07-06 17:24:43,858:-------------------------------------------------------- INFO(139945971238720)2022-07-06 17:24:43,858:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-21_20_L001_R1_001.fastq.gz INFO(140439722145600)2022-07-06 17:31:53,904:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-23_22_L001_R1_001.fastq.gz.trim INFO(140439722145600)2022-07-06 17:32:26,135:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-23_22_L001_R1_001.fastq.gz.trim.derep INFO(140439722145600)2022-07-06 17:32:53,188:total sequences 778345, passing sequences 778345, failing sequences 0 INFO(140439722145600)2022-07-06 17:32:53,188:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-23_22_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140272526374720)2022-07-06 17:59:56,888:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-04_3_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140272526374720)2022-07-06 17:59:58,657:msa failed. aborting WARNING(140272526374720)2022-07-06 17:59:58,690:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-04_3_L001_R1_001.fastq.gz INFO(140272526374720)2022-07-06 17:59:58,690:finished processing per sample fasta files INFO(140272526374720)2022-07-06 17:59:58,690:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-04_3_L001_R1_001.fastq.gz finished INFO(139754469713728)2022-07-06 18:00:28,500:************************* INFO(139754469713728)2022-07-06 18:00:28,500:deblurring started WARNING(139754469713728)2022-07-06 18:00:28,500:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-05_4_L001_R1_001.fastq.gz WARNING(139754469713728)2022-07-06 18:00:28,500:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-05_4_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139754469713728)2022-07-06 18:00:28,500:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139754469713728)2022-07-06 18:00:28,500:deblur worker thread started INFO(139754469713728)2022-07-06 18:00:28,500:processing per sample fasta files INFO(139754469713728)2022-07-06 18:00:28,500:-------------------------------------------------------- INFO(139754469713728)2022-07-06 18:00:28,500:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-05_4_L001_R1_001.fastq.gz INFO(139945971238720)2022-07-06 18:25:33,597:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-21_20_L001_R1_001.fastq.gz.trim INFO(139945971238720)2022-07-06 18:26:13,947:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-21_20_L001_R1_001.fastq.gz.trim.derep INFO(139945971238720)2022-07-06 18:27:11,364:total sequences 1568236, passing sequences 1568236, failing sequences 0 INFO(139945971238720)2022-07-06 18:27:11,364:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-21_20_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140426519250752)2022-07-06 18:45:32,864:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-35_34_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140426519250752)2022-07-06 18:45:34,161:msa failed. aborting WARNING(140426519250752)2022-07-06 18:45:34,205:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-35_34_L001_R1_001.fastq.gz INFO(140426519250752)2022-07-06 18:45:34,218:finished processing per sample fasta files INFO(140426519250752)2022-07-06 18:45:34,218:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-35_34_L001_R1_001.fastq.gz finished INFO(139793360508736)2022-07-06 18:46:03,208:************************* INFO(139793360508736)2022-07-06 18:46:03,208:deblurring started WARNING(139793360508736)2022-07-06 18:46:03,208:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-36_35_L001_R1_001.fastq.gz WARNING(139793360508736)2022-07-06 18:46:03,208:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-36_35_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139793360508736)2022-07-06 18:46:03,208:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139793360508736)2022-07-06 18:46:03,208:deblur worker thread started INFO(139793360508736)2022-07-06 18:46:03,208:processing per sample fasta files INFO(139793360508736)2022-07-06 18:46:03,208:-------------------------------------------------------- INFO(139793360508736)2022-07-06 18:46:03,208:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-36_35_L001_R1_001.fastq.gz INFO(139754469713728)2022-07-06 19:07:10,543:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-05_4_L001_R1_001.fastq.gz.trim INFO(139754469713728)2022-07-06 19:08:02,074:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-05_4_L001_R1_001.fastq.gz.trim.derep INFO(139754469713728)2022-07-06 19:08:58,944:total sequences 1547581, passing sequences 1547581, failing sequences 0 INFO(139754469713728)2022-07-06 19:08:58,944:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-05_4_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139798669473600)2022-07-06 19:11:42,487:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-24_23_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139798669473600)2022-07-06 19:11:43,324:msa failed. aborting WARNING(139798669473600)2022-07-06 19:11:43,443:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-24_23_L001_R1_001.fastq.gz INFO(139798669473600)2022-07-06 19:11:43,452:finished processing per sample fasta files INFO(139798669473600)2022-07-06 19:11:43,452:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-24_23_L001_R1_001.fastq.gz finished INFO(139874910902080)2022-07-06 19:12:12,504:************************* INFO(139874910902080)2022-07-06 19:12:12,504:deblurring started WARNING(139874910902080)2022-07-06 19:12:12,505:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-02_1_L001_R1_001.fastq.gz WARNING(139874910902080)2022-07-06 19:12:12,505:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-02_1_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139874910902080)2022-07-06 19:12:12,505:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139874910902080)2022-07-06 19:12:12,505:deblur worker thread started INFO(139874910902080)2022-07-06 19:12:12,505:processing per sample fasta files INFO(139874910902080)2022-07-06 19:12:12,505:-------------------------------------------------------- INFO(139874910902080)2022-07-06 19:12:12,505:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-02_1_L001_R1_001.fastq.gz INFO(139793360508736)2022-07-06 19:27:43,426:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-36_35_L001_R1_001.fastq.gz.trim INFO(139793360508736)2022-07-06 19:28:15,531:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-36_35_L001_R1_001.fastq.gz.trim.derep INFO(139793360508736)2022-07-06 19:28:41,732:total sequences 675275, passing sequences 675262, failing sequences 13 INFO(139793360508736)2022-07-06 19:28:41,732:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-36_35_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139874910902080)2022-07-06 20:16:47,719:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-02_1_L001_R1_001.fastq.gz.trim INFO(139874910902080)2022-07-06 20:17:35,319:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-02_1_L001_R1_001.fastq.gz.trim.derep INFO(139874910902080)2022-07-06 20:18:32,055:total sequences 1481607, passing sequences 1481607, failing sequences 0 INFO(139874910902080)2022-07-06 20:18:32,055:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-02_1_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140119843374912)2022-07-06 20:20:44,743:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-28_27_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140119843374912)2022-07-06 20:20:45,568:msa failed. aborting WARNING(140119843374912)2022-07-06 20:20:45,671:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-28_27_L001_R1_001.fastq.gz INFO(140119843374912)2022-07-06 20:20:45,671:finished processing per sample fasta files INFO(140119843374912)2022-07-06 20:20:45,671:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-28_27_L001_R1_001.fastq.gz finished INFO(140271312578368)2022-07-06 20:21:08,654:************************* INFO(140271312578368)2022-07-06 20:21:08,654:deblurring started WARNING(140271312578368)2022-07-06 20:21:08,654:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-08_7_L001_R1_001.fastq.gz WARNING(140271312578368)2022-07-06 20:21:08,654:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-08_7_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140271312578368)2022-07-06 20:21:08,654:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140271312578368)2022-07-06 20:21:08,654:deblur worker thread started INFO(140271312578368)2022-07-06 20:21:08,654:processing per sample fasta files INFO(140271312578368)2022-07-06 20:21:08,654:-------------------------------------------------------- INFO(140271312578368)2022-07-06 20:21:08,654:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-08_7_L001_R1_001.fastq.gz INFO(140401463691072)2022-07-06 20:41:48,011:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-29_28_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140401463691072)2022-07-06 20:41:52,712:msa failed. aborting WARNING(140401463691072)2022-07-06 20:41:53,340:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-29_28_L001_R1_001.fastq.gz INFO(140401463691072)2022-07-06 20:41:53,340:finished processing per sample fasta files INFO(140401463691072)2022-07-06 20:41:53,340:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-29_28_L001_R1_001.fastq.gz finished INFO(140351539808064)2022-07-06 20:42:31,615:************************* INFO(140351539808064)2022-07-06 20:42:31,615:deblurring started WARNING(140351539808064)2022-07-06 20:42:31,616:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-25_24_L001_R1_001.fastq.gz WARNING(140351539808064)2022-07-06 20:42:31,616:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-25_24_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140351539808064)2022-07-06 20:42:31,616:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140351539808064)2022-07-06 20:42:31,616:deblur worker thread started INFO(140351539808064)2022-07-06 20:42:31,616:processing per sample fasta files INFO(140351539808064)2022-07-06 20:42:31,616:-------------------------------------------------------- INFO(140351539808064)2022-07-06 20:42:31,616:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-25_24_L001_R1_001.fastq.gz INFO(139811369133888)2022-07-06 21:01:12,342:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-10_9_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139811369133888)2022-07-06 21:01:13,083:msa failed. aborting WARNING(139811369133888)2022-07-06 21:01:13,083:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-10_9_L001_R1_001.fastq.gz INFO(139811369133888)2022-07-06 21:01:13,083:finished processing per sample fasta files INFO(139811369133888)2022-07-06 21:01:13,083:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-10_9_L001_R1_001.fastq.gz finished INFO(139876271486784)2022-07-06 21:01:26,857:************************* INFO(139876271486784)2022-07-06 21:01:26,857:deblurring started WARNING(139876271486784)2022-07-06 21:01:26,857:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-27_26_L001_R1_001.fastq.gz WARNING(139876271486784)2022-07-06 21:01:26,857:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-27_26_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139876271486784)2022-07-06 21:01:26,857:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139876271486784)2022-07-06 21:01:26,857:deblur worker thread started INFO(139876271486784)2022-07-06 21:01:26,857:processing per sample fasta files INFO(139876271486784)2022-07-06 21:01:26,857:-------------------------------------------------------- INFO(139876271486784)2022-07-06 21:01:26,857:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-27_26_L001_R1_001.fastq.gz INFO(140439722145600)2022-07-06 21:06:54,922:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-23_22_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140439722145600)2022-07-06 21:06:55,602:msa failed. aborting WARNING(140439722145600)2022-07-06 21:06:55,604:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-23_22_L001_R1_001.fastq.gz INFO(140439722145600)2022-07-06 21:06:55,604:finished processing per sample fasta files INFO(140439722145600)2022-07-06 21:06:55,604:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-23_22_L001_R1_001.fastq.gz finished INFO(140231828834112)2022-07-06 21:07:15,757:************************* INFO(140231828834112)2022-07-06 21:07:15,757:deblurring started WARNING(140231828834112)2022-07-06 21:07:15,757:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-18_17_L001_R1_001.fastq.gz WARNING(140231828834112)2022-07-06 21:07:15,757:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-18_17_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140231828834112)2022-07-06 21:07:15,757:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140231828834112)2022-07-06 21:07:15,757:deblur worker thread started INFO(140231828834112)2022-07-06 21:07:15,757:processing per sample fasta files INFO(140231828834112)2022-07-06 21:07:15,757:-------------------------------------------------------- INFO(140231828834112)2022-07-06 21:07:15,758:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-18_17_L001_R1_001.fastq.gz INFO(140098394359616)2022-07-06 21:34:28,692:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140098394359616)2022-07-06 21:34:29,537:msa failed. aborting WARNING(140098394359616)2022-07-06 21:34:29,594:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-11_10_L001_R1_001.fastq.gz INFO(140098394359616)2022-07-06 21:34:29,599:finished processing per sample fasta files INFO(140098394359616)2022-07-06 21:34:29,599:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-11_10_L001_R1_001.fastq.gz finished INFO(140026115233600)2022-07-06 21:35:02,110:************************* INFO(140026115233600)2022-07-06 21:35:02,111:deblurring started WARNING(140026115233600)2022-07-06 21:35:02,111:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-20_19_L001_R1_001.fastq.gz WARNING(140026115233600)2022-07-06 21:35:02,111:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-20_19_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140026115233600)2022-07-06 21:35:02,111:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140026115233600)2022-07-06 21:35:02,111:deblur worker thread started INFO(140026115233600)2022-07-06 21:35:02,111:processing per sample fasta files INFO(140026115233600)2022-07-06 21:35:02,111:-------------------------------------------------------- INFO(140026115233600)2022-07-06 21:35:02,111:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-20_19_L001_R1_001.fastq.gz INFO(140271312578368)2022-07-06 21:37:18,139:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-08_7_L001_R1_001.fastq.gz.trim INFO(140271312578368)2022-07-06 21:38:14,878:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-08_7_L001_R1_001.fastq.gz.trim.derep INFO(140271312578368)2022-07-06 21:39:32,273:total sequences 1885582, passing sequences 1885582, failing sequences 0 INFO(140271312578368)2022-07-06 21:39:32,273:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-08_7_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140351539808064)2022-07-06 21:41:06,468:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-25_24_L001_R1_001.fastq.gz.trim INFO(140351539808064)2022-07-06 21:41:45,779:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-25_24_L001_R1_001.fastq.gz.trim.derep INFO(140351539808064)2022-07-06 21:42:38,758:total sequences 1312262, passing sequences 1312262, failing sequences 0 INFO(140351539808064)2022-07-06 21:42:38,758:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-25_24_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139876271486784)2022-07-06 21:50:39,482:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-27_26_L001_R1_001.fastq.gz.trim INFO(139876271486784)2022-07-06 21:51:10,897:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-27_26_L001_R1_001.fastq.gz.trim.derep INFO(139876271486784)2022-07-06 21:51:45,711:total sequences 966403, passing sequences 966403, failing sequences 0 INFO(139876271486784)2022-07-06 21:51:45,711:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-27_26_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140026115233600)2022-07-06 22:12:01,489:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-20_19_L001_R1_001.fastq.gz.trim INFO(140026115233600)2022-07-06 22:12:17,275:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-20_19_L001_R1_001.fastq.gz.trim.derep INFO(140231828834112)2022-07-06 22:12:37,645:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-18_17_L001_R1_001.fastq.gz.trim INFO(140026115233600)2022-07-06 22:12:42,109:total sequences 896674, passing sequences 896674, failing sequences 0 INFO(140026115233600)2022-07-06 22:12:42,109:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-20_19_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140231828834112)2022-07-06 22:13:36,957:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-18_17_L001_R1_001.fastq.gz.trim.derep INFO(140231828834112)2022-07-06 22:14:39,122:total sequences 1736821, passing sequences 1736821, failing sequences 0 INFO(140231828834112)2022-07-06 22:14:39,122:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-18_17_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140100837398336)2022-07-06 22:29:56,049:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-30_29_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140100837398336)2022-07-06 22:29:56,515:msa failed. aborting WARNING(140100837398336)2022-07-06 22:29:56,543:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-30_29_L001_R1_001.fastq.gz INFO(140100837398336)2022-07-06 22:29:56,543:finished processing per sample fasta files INFO(140100837398336)2022-07-06 22:29:56,543:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-30_29_L001_R1_001.fastq.gz finished INFO(140533242980160)2022-07-06 22:30:13,760:************************* INFO(140533242980160)2022-07-06 22:30:13,760:deblurring started WARNING(140533242980160)2022-07-06 22:30:13,760:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-12_11_L001_R1_001.fastq.gz WARNING(140533242980160)2022-07-06 22:30:13,760:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-12_11_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140533242980160)2022-07-06 22:30:13,760:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140533242980160)2022-07-06 22:30:13,760:deblur worker thread started INFO(140533242980160)2022-07-06 22:30:13,760:processing per sample fasta files INFO(140533242980160)2022-07-06 22:30:13,760:-------------------------------------------------------- INFO(140533242980160)2022-07-06 22:30:13,760:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-12_11_L001_R1_001.fastq.gz INFO(139793360508736)2022-07-06 23:02:38,788:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-36_35_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139793360508736)2022-07-06 23:02:39,752:msa failed. aborting WARNING(139793360508736)2022-07-06 23:02:39,752:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-36_35_L001_R1_001.fastq.gz INFO(139793360508736)2022-07-06 23:02:39,752:finished processing per sample fasta files INFO(139793360508736)2022-07-06 23:02:39,752:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-36_35_L001_R1_001.fastq.gz finished INFO(139743586051904)2022-07-06 23:03:00,070:************************* INFO(139743586051904)2022-07-06 23:03:00,070:deblurring started WARNING(139743586051904)2022-07-06 23:03:00,070:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-15_14_L001_R1_001.fastq.gz WARNING(139743586051904)2022-07-06 23:03:00,070:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-15_14_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139743586051904)2022-07-06 23:03:00,070:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139743586051904)2022-07-06 23:03:00,070:deblur worker thread started INFO(139743586051904)2022-07-06 23:03:00,070:processing per sample fasta files INFO(139743586051904)2022-07-06 23:03:00,070:-------------------------------------------------------- INFO(139743586051904)2022-07-06 23:03:00,070:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-15_14_L001_R1_001.fastq.gz INFO(140533242980160)2022-07-06 23:23:32,105:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-12_11_L001_R1_001.fastq.gz.trim INFO(140533242980160)2022-07-06 23:24:09,313:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-12_11_L001_R1_001.fastq.gz.trim.derep INFO(140533242980160)2022-07-06 23:24:59,365:total sequences 1322040, passing sequences 1322040, failing sequences 0 INFO(140533242980160)2022-07-06 23:24:59,365:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-12_11_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139743586051904)2022-07-06 23:48:05,361:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-15_14_L001_R1_001.fastq.gz.trim INFO(139743586051904)2022-07-06 23:48:39,380:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-15_14_L001_R1_001.fastq.gz.trim.derep INFO(139743586051904)2022-07-06 23:49:17,668:total sequences 1005882, passing sequences 1005882, failing sequences 0 INFO(139743586051904)2022-07-06 23:49:17,668:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-15_14_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139754469713728)2022-07-06 23:54:07,777:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-05_4_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139754469713728)2022-07-06 23:54:08,158:msa failed. aborting WARNING(139754469713728)2022-07-06 23:54:08,158:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-05_4_L001_R1_001.fastq.gz INFO(139754469713728)2022-07-06 23:54:08,158:finished processing per sample fasta files INFO(139754469713728)2022-07-06 23:54:08,158:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-05_4_L001_R1_001.fastq.gz finished INFO(139729478514496)2022-07-06 23:54:22,213:************************* INFO(139729478514496)2022-07-06 23:54:22,213:deblurring started WARNING(139729478514496)2022-07-06 23:54:22,213:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-14_13_L001_R1_001.fastq.gz WARNING(139729478514496)2022-07-06 23:54:22,213:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-14_13_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139729478514496)2022-07-06 23:54:22,213:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139729478514496)2022-07-06 23:54:22,213:deblur worker thread started INFO(139729478514496)2022-07-06 23:54:22,213:processing per sample fasta files INFO(139729478514496)2022-07-06 23:54:22,213:-------------------------------------------------------- INFO(139729478514496)2022-07-06 23:54:22,213:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-14_13_L001_R1_001.fastq.gz INFO(139874910902080)2022-07-07 00:33:48,619:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-02_1_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139874910902080)2022-07-07 00:33:49,073:msa failed. aborting WARNING(139874910902080)2022-07-07 00:33:49,073:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-02_1_L001_R1_001.fastq.gz INFO(139874910902080)2022-07-07 00:33:49,073:finished processing per sample fasta files INFO(139874910902080)2022-07-07 00:33:49,073:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-02_1_L001_R1_001.fastq.gz finished INFO(139674534016832)2022-07-07 00:34:05,366:************************* INFO(139674534016832)2022-07-07 00:34:05,366:deblurring started WARNING(139674534016832)2022-07-07 00:34:05,366:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-26_25_L001_R1_001.fastq.gz WARNING(139674534016832)2022-07-07 00:34:05,366:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-26_25_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139674534016832)2022-07-07 00:34:05,366:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139674534016832)2022-07-07 00:34:05,366:deblur worker thread started INFO(139674534016832)2022-07-07 00:34:05,366:processing per sample fasta files INFO(139674534016832)2022-07-07 00:34:05,366:-------------------------------------------------------- INFO(139674534016832)2022-07-07 00:34:05,366:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-26_25_L001_R1_001.fastq.gz INFO(139729478514496)2022-07-07 00:52:36,007:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-14_13_L001_R1_001.fastq.gz.trim INFO(139729478514496)2022-07-07 00:53:19,714:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-14_13_L001_R1_001.fastq.gz.trim.derep INFO(139729478514496)2022-07-07 00:54:18,608:total sequences 1563911, passing sequences 1563911, failing sequences 0 INFO(139729478514496)2022-07-07 00:54:18,608:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-14_13_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140351539808064)2022-07-07 01:14:33,365:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-25_24_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140351539808064)2022-07-07 01:14:33,578:msa failed. aborting WARNING(140351539808064)2022-07-07 01:14:33,578:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-25_24_L001_R1_001.fastq.gz INFO(140351539808064)2022-07-07 01:14:33,578:finished processing per sample fasta files INFO(140351539808064)2022-07-07 01:14:33,578:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-25_24_L001_R1_001.fastq.gz finished INFO(140134444861248)2022-07-07 01:14:45,737:************************* INFO(140134444861248)2022-07-07 01:14:45,737:deblurring started WARNING(140134444861248)2022-07-07 01:14:45,737:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-16_15_L001_R1_001.fastq.gz WARNING(140134444861248)2022-07-07 01:14:45,737:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-16_15_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140134444861248)2022-07-07 01:14:45,737:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140134444861248)2022-07-07 01:14:45,737:deblur worker thread started INFO(140134444861248)2022-07-07 01:14:45,737:processing per sample fasta files INFO(140134444861248)2022-07-07 01:14:45,737:-------------------------------------------------------- INFO(140134444861248)2022-07-07 01:14:45,737:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-16_15_L001_R1_001.fastq.gz INFO(139674534016832)2022-07-07 01:43:46,874:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-26_25_L001_R1_001.fastq.gz.trim INFO(139674534016832)2022-07-07 01:44:44,017:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-26_25_L001_R1_001.fastq.gz.trim.derep INFO(139674534016832)2022-07-07 01:45:40,063:total sequences 1528285, passing sequences 1528285, failing sequences 0 INFO(139674534016832)2022-07-07 01:45:40,063:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-26_25_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139945971238720)2022-07-07 01:53:06,107:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-21_20_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139945971238720)2022-07-07 01:53:07,887:msa failed. aborting WARNING(139945971238720)2022-07-07 01:53:07,970:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-21_20_L001_R1_001.fastq.gz INFO(139945971238720)2022-07-07 01:53:07,970:finished processing per sample fasta files INFO(139945971238720)2022-07-07 01:53:07,970:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-21_20_L001_R1_001.fastq.gz finished INFO(140042835203904)2022-07-07 01:53:25,141:************************* INFO(140042835203904)2022-07-07 01:53:25,141:deblurring started WARNING(140042835203904)2022-07-07 01:53:25,142:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-19_18_L001_R1_001.fastq.gz WARNING(140042835203904)2022-07-07 01:53:25,142:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-19_18_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140042835203904)2022-07-07 01:53:25,142:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140042835203904)2022-07-07 01:53:25,142:deblur worker thread started INFO(140042835203904)2022-07-07 01:53:25,142:processing per sample fasta files INFO(140042835203904)2022-07-07 01:53:25,142:-------------------------------------------------------- INFO(140042835203904)2022-07-07 01:53:25,142:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-19_18_L001_R1_001.fastq.gz INFO(140134444861248)2022-07-07 02:08:21,151:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-16_15_L001_R1_001.fastq.gz.trim INFO(140134444861248)2022-07-07 02:08:56,307:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-16_15_L001_R1_001.fastq.gz.trim.derep INFO(140134444861248)2022-07-07 02:09:41,506:total sequences 1190115, passing sequences 1190113, failing sequences 2 INFO(140134444861248)2022-07-07 02:09:41,506:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-16_15_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140271312578368)2022-07-07 02:33:39,138:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-08_7_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140271312578368)2022-07-07 02:33:39,632:msa failed. aborting WARNING(140271312578368)2022-07-07 02:33:39,632:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-08_7_L001_R1_001.fastq.gz INFO(140271312578368)2022-07-07 02:33:39,632:finished processing per sample fasta files INFO(140271312578368)2022-07-07 02:33:39,633:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-08_7_L001_R1_001.fastq.gz finished INFO(139888969811776)2022-07-07 02:33:53,095:************************* INFO(139888969811776)2022-07-07 02:33:53,095:deblurring started WARNING(139888969811776)2022-07-07 02:33:53,095:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-03_2_L001_R1_001.fastq.gz WARNING(139888969811776)2022-07-07 02:33:53,096:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-03_2_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139888969811776)2022-07-07 02:33:53,096:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139888969811776)2022-07-07 02:33:53,096:deblur worker thread started INFO(139888969811776)2022-07-07 02:33:53,096:processing per sample fasta files INFO(139888969811776)2022-07-07 02:33:53,096:-------------------------------------------------------- INFO(139888969811776)2022-07-07 02:33:53,096:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-03_2_L001_R1_001.fastq.gz INFO(139876271486784)2022-07-07 02:41:22,989:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-27_26_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139876271486784)2022-07-07 02:41:23,499:msa failed. aborting WARNING(139876271486784)2022-07-07 02:41:23,500:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-27_26_L001_R1_001.fastq.gz INFO(139876271486784)2022-07-07 02:41:23,500:finished processing per sample fasta files INFO(139876271486784)2022-07-07 02:41:23,500:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-27_26_L001_R1_001.fastq.gz finished INFO(139980882188096)2022-07-07 02:41:36,941:************************* INFO(139980882188096)2022-07-07 02:41:36,941:deblurring started WARNING(139980882188096)2022-07-07 02:41:36,942:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-22_21_L001_R1_001.fastq.gz WARNING(139980882188096)2022-07-07 02:41:36,942:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-22_21_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139980882188096)2022-07-07 02:41:36,942:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139980882188096)2022-07-07 02:41:36,942:deblur worker thread started INFO(139980882188096)2022-07-07 02:41:36,942:processing per sample fasta files INFO(139980882188096)2022-07-07 02:41:36,942:-------------------------------------------------------- INFO(139980882188096)2022-07-07 02:41:36,942:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-22_21_L001_R1_001.fastq.gz INFO(140026115233600)2022-07-07 02:51:15,654:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-20_19_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140026115233600)2022-07-07 02:51:16,076:msa failed. aborting WARNING(140026115233600)2022-07-07 02:51:16,077:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-20_19_L001_R1_001.fastq.gz INFO(140026115233600)2022-07-07 02:51:16,077:finished processing per sample fasta files INFO(140026115233600)2022-07-07 02:51:16,077:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-20_19_L001_R1_001.fastq.gz finished INFO(139983740086080)2022-07-07 02:51:29,806:************************* INFO(139983740086080)2022-07-07 02:51:29,806:deblurring started WARNING(139983740086080)2022-07-07 02:51:29,806:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-06_5_L001_R1_001.fastq.gz WARNING(139983740086080)2022-07-07 02:51:29,806:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-06_5_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139983740086080)2022-07-07 02:51:29,806:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139983740086080)2022-07-07 02:51:29,806:deblur worker thread started INFO(139983740086080)2022-07-07 02:51:29,806:processing per sample fasta files INFO(139983740086080)2022-07-07 02:51:29,806:-------------------------------------------------------- INFO(139983740086080)2022-07-07 02:51:29,806:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-06_5_L001_R1_001.fastq.gz INFO(140042835203904)2022-07-07 02:52:03,069:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-19_18_L001_R1_001.fastq.gz.trim INFO(140042835203904)2022-07-07 02:52:50,229:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-19_18_L001_R1_001.fastq.gz.trim.derep INFO(140042835203904)2022-07-07 02:53:43,711:total sequences 1442551, passing sequences 1442551, failing sequences 0 INFO(140042835203904)2022-07-07 02:53:43,711:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-19_18_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139888969811776)2022-07-07 03:22:08,307:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-03_2_L001_R1_001.fastq.gz.trim INFO(139888969811776)2022-07-07 03:22:39,663:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-03_2_L001_R1_001.fastq.gz.trim.derep INFO(139888969811776)2022-07-07 03:23:22,580:total sequences 1051530, passing sequences 1051527, failing sequences 3 INFO(139888969811776)2022-07-07 03:23:22,629:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-03_2_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140231828834112)2022-07-07 03:36:43,622:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-18_17_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140231828834112)2022-07-07 03:36:44,088:msa failed. aborting WARNING(140231828834112)2022-07-07 03:36:44,089:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-18_17_L001_R1_001.fastq.gz INFO(140231828834112)2022-07-07 03:36:44,089:finished processing per sample fasta files INFO(140231828834112)2022-07-07 03:36:44,089:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-18_17_L001_R1_001.fastq.gz finished INFO(140569253181248)2022-07-07 03:36:57,784:************************* INFO(140569253181248)2022-07-07 03:36:57,784:deblurring started WARNING(140569253181248)2022-07-07 03:36:57,785:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-07_6_L001_R1_001.fastq.gz WARNING(140569253181248)2022-07-07 03:36:57,785:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-07_6_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140569253181248)2022-07-07 03:36:57,785:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140569253181248)2022-07-07 03:36:57,785:deblur worker thread started INFO(140569253181248)2022-07-07 03:36:57,785:processing per sample fasta files INFO(140569253181248)2022-07-07 03:36:57,785:-------------------------------------------------------- INFO(140569253181248)2022-07-07 03:36:57,785:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-07_6_L001_R1_001.fastq.gz INFO(139980882188096)2022-07-07 03:47:12,336:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-22_21_L001_R1_001.fastq.gz.trim INFO(139980882188096)2022-07-07 03:48:01,147:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-22_21_L001_R1_001.fastq.gz.trim.derep INFO(139980882188096)2022-07-07 03:48:52,354:total sequences 1393553, passing sequences 1393553, failing sequences 0 INFO(139980882188096)2022-07-07 03:48:52,354:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-22_21_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139983740086080)2022-07-07 03:51:15,749:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-06_5_L001_R1_001.fastq.gz.trim INFO(139983740086080)2022-07-07 03:51:59,040:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-06_5_L001_R1_001.fastq.gz.trim.derep INFO(139983740086080)2022-07-07 03:52:43,810:total sequences 1174920, passing sequences 1174920, failing sequences 0 INFO(139983740086080)2022-07-07 03:52:43,811:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-06_5_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139729478514496)2022-07-07 04:13:31,244:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-14_13_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139729478514496)2022-07-07 04:13:31,701:msa failed. aborting WARNING(139729478514496)2022-07-07 04:13:31,701:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-14_13_L001_R1_001.fastq.gz INFO(139729478514496)2022-07-07 04:13:31,701:finished processing per sample fasta files INFO(139729478514496)2022-07-07 04:13:31,701:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-14_13_L001_R1_001.fastq.gz finished INFO(140299450910528)2022-07-07 04:13:47,595:************************* INFO(140299450910528)2022-07-07 04:13:47,596:deblurring started WARNING(140299450910528)2022-07-07 04:13:47,596:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-32_31_L001_R1_001.fastq.gz WARNING(140299450910528)2022-07-07 04:13:47,596:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-32_31_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140299450910528)2022-07-07 04:13:47,596:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140299450910528)2022-07-07 04:13:47,596:deblur worker thread started INFO(140299450910528)2022-07-07 04:13:47,596:processing per sample fasta files INFO(140299450910528)2022-07-07 04:13:47,596:-------------------------------------------------------- INFO(140299450910528)2022-07-07 04:13:47,596:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-32_31_L001_R1_001.fastq.gz INFO(140569253181248)2022-07-07 04:38:29,932:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-07_6_L001_R1_001.fastq.gz.trim INFO(140569253181248)2022-07-07 04:39:17,615:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-07_6_L001_R1_001.fastq.gz.trim.derep INFO(140569253181248)2022-07-07 04:40:24,933:total sequences 1546561, passing sequences 1546561, failing sequences 0 INFO(140569253181248)2022-07-07 04:40:24,933:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-07_6_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139674534016832)2022-07-07 04:43:42,070:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-26_25_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139674534016832)2022-07-07 04:43:42,253:msa failed. aborting WARNING(139674534016832)2022-07-07 04:43:42,253:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-26_25_L001_R1_001.fastq.gz INFO(139674534016832)2022-07-07 04:43:42,253:finished processing per sample fasta files INFO(139674534016832)2022-07-07 04:43:42,253:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-26_25_L001_R1_001.fastq.gz finished INFO(140670405642048)2022-07-07 04:43:53,760:************************* INFO(140670405642048)2022-07-07 04:43:53,760:deblurring started WARNING(140670405642048)2022-07-07 04:43:53,760:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-17_16_L001_R1_001.fastq.gz WARNING(140670405642048)2022-07-07 04:43:53,760:parameters: {'seqs_fp': '/tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-17_16_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmpns9tipf_', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmpns9tipf_/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': -1, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140670405642048)2022-07-07 04:43:53,760:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140670405642048)2022-07-07 04:43:53,761:deblur worker thread started INFO(140670405642048)2022-07-07 04:43:53,761:processing per sample fasta files INFO(140670405642048)2022-07-07 04:43:53,761:-------------------------------------------------------- INFO(140670405642048)2022-07-07 04:43:53,761:launch_workflow for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-17_16_L001_R1_001.fastq.gz INFO(139743586051904)2022-07-07 04:55:06,103:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-15_14_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139743586051904)2022-07-07 04:55:06,437:msa failed. aborting WARNING(139743586051904)2022-07-07 04:55:06,437:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-15_14_L001_R1_001.fastq.gz INFO(139743586051904)2022-07-07 04:55:06,437:finished processing per sample fasta files INFO(139743586051904)2022-07-07 04:55:06,437:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-15_14_L001_R1_001.fastq.gz finished INFO(140299450910528)2022-07-07 05:09:56,910:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-32_31_L001_R1_001.fastq.gz.trim INFO(140299450910528)2022-07-07 05:10:35,269:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-32_31_L001_R1_001.fastq.gz.trim.derep INFO(140299450910528)2022-07-07 05:11:29,730:total sequences 1398058, passing sequences 1398058, failing sequences 0 INFO(140299450910528)2022-07-07 05:11:29,730:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-32_31_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140533242980160)2022-07-07 05:44:47,790:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-12_11_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140533242980160)2022-07-07 05:44:48,024:msa failed. aborting WARNING(140533242980160)2022-07-07 05:44:48,024:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-12_11_L001_R1_001.fastq.gz INFO(140533242980160)2022-07-07 05:44:48,024:finished processing per sample fasta files INFO(140533242980160)2022-07-07 05:44:48,024:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-12_11_L001_R1_001.fastq.gz finished INFO(140670405642048)2022-07-07 05:48:04,789:dereplicate seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-17_16_L001_R1_001.fastq.gz.trim INFO(140670405642048)2022-07-07 05:48:53,126:remove_artifacts_seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-17_16_L001_R1_001.fastq.gz.trim.derep INFO(140670405642048)2022-07-07 05:49:50,612:total sequences 1559327, passing sequences 1559327, failing sequences 0 INFO(140670405642048)2022-07-07 05:49:50,612:multiple_sequence_alignment seqs file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-17_16_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139980882188096)2022-07-07 07:04:00,980:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-22_21_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139980882188096)2022-07-07 07:04:01,967:msa failed. aborting WARNING(139980882188096)2022-07-07 07:04:01,967:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-22_21_L001_R1_001.fastq.gz INFO(139980882188096)2022-07-07 07:04:01,967:finished processing per sample fasta files INFO(139980882188096)2022-07-07 07:04:01,967:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-22_21_L001_R1_001.fastq.gz finished INFO(140134444861248)2022-07-07 07:31:32,597:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-16_15_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140134444861248)2022-07-07 07:31:33,240:msa failed. aborting WARNING(140134444861248)2022-07-07 07:31:33,240:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-16_15_L001_R1_001.fastq.gz INFO(140134444861248)2022-07-07 07:31:33,240:finished processing per sample fasta files INFO(140134444861248)2022-07-07 07:31:33,240:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-16_15_L001_R1_001.fastq.gz finished INFO(140299450910528)2022-07-07 08:04:33,504:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-32_31_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140299450910528)2022-07-07 08:04:34,380:msa failed. aborting WARNING(140299450910528)2022-07-07 08:04:34,380:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-32_31_L001_R1_001.fastq.gz INFO(140299450910528)2022-07-07 08:04:34,380:finished processing per sample fasta files INFO(140299450910528)2022-07-07 08:04:34,380:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-32_31_L001_R1_001.fastq.gz finished INFO(139888969811776)2022-07-07 08:16:41,964:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-03_2_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139888969811776)2022-07-07 08:16:42,430:msa failed. aborting WARNING(139888969811776)2022-07-07 08:16:42,430:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-03_2_L001_R1_001.fastq.gz INFO(139888969811776)2022-07-07 08:16:42,430:finished processing per sample fasta files INFO(139888969811776)2022-07-07 08:16:42,430:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-03_2_L001_R1_001.fastq.gz finished INFO(140042835203904)2022-07-07 08:41:40,771:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-19_18_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140042835203904)2022-07-07 08:41:41,182:msa failed. aborting WARNING(140042835203904)2022-07-07 08:41:41,182:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-19_18_L001_R1_001.fastq.gz INFO(140042835203904)2022-07-07 08:41:41,182:finished processing per sample fasta files INFO(140042835203904)2022-07-07 08:41:41,182:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-19_18_L001_R1_001.fastq.gz finished INFO(139983740086080)2022-07-07 09:03:36,964:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-06_5_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139983740086080)2022-07-07 09:03:37,595:msa failed. aborting WARNING(139983740086080)2022-07-07 09:03:37,595:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-06_5_L001_R1_001.fastq.gz INFO(139983740086080)2022-07-07 09:03:37,595:finished processing per sample fasta files INFO(139983740086080)2022-07-07 09:03:37,595:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-06_5_L001_R1_001.fastq.gz finished INFO(140569253181248)2022-07-07 11:01:47,641:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-07_6_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140569253181248)2022-07-07 11:01:49,625:msa failed. aborting WARNING(140569253181248)2022-07-07 11:01:49,625:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-07_6_L001_R1_001.fastq.gz INFO(140569253181248)2022-07-07 11:01:49,625:finished processing per sample fasta files INFO(140569253181248)2022-07-07 11:01:49,625:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-07_6_L001_R1_001.fastq.gz finished INFO(140670405642048)2022-07-07 12:34:42,389:msa failed for file /tmp/tmpns9tipf_/deblur_working_dir/MICRO-8855-17_16_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140670405642048)2022-07-07 12:34:42,669:msa failed. aborting WARNING(140670405642048)2022-07-07 12:34:42,669:deblurring failed for file /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-17_16_L001_R1_001.fastq.gz INFO(140670405642048)2022-07-07 12:34:42,669:finished processing per sample fasta files INFO(140670405642048)2022-07-07 12:34:42,669:worker thread for /tmp/qiime2-archive-_8i_gimy/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-17_16_L001_R1_001.fastq.gz finished INFO(139792277886784)2022-07-07 12:34:48,053:create_otu_table for 0 samples, into output table /tmp/tmpns9tipf_/all.biom INFO(139792277886784)2022-07-07 12:34:48,070:for output biom table loaded 0 samples, 0 unique sequences INFO(139792277886784)2022-07-07 12:34:48,089:keeping 0 (out of 0 sequences) with >=10 reads INFO(139792277886784)2022-07-07 12:34:48,234:saved to biom file /tmp/tmpns9tipf_/all.biom INFO(139792277886784)2022-07-07 12:34:48,234:saved sequence fasta file to /tmp/tmpns9tipf_/all.seqs.fa INFO(139792277886784)2022-07-07 12:34:48,234:getting 16s sequences from the biom table INFO(139792277886784)2022-07-07 12:34:48,234:remove_artifacts_seqs file /tmp/tmpns9tipf_/all.seqs.fa WARNING(139792277886784)2022-07-07 12:34:48,234:file /tmp/tmpns9tipf_/all.seqs.fa has size 0, continuing WARNING(139792277886784)2022-07-07 12:34:48,234:No clean sequences in /tmp/tmpns9tipf_/all.seqs.fa INFO(139792277886784)2022-07-07 12:34:48,234:Keeping temp files INFO(139792277886784)2022-07-07 12:34:48,234:deblur workflow finished INFO(139792277886784)2022-07-07 12:34:48,234:output saved to /tmp/tmpns9tipf_/all.biom INFO(139792277886784)2022-07-07 12:34:48,234:------------------ INFO(139987148605248)2022-07-07 16:14:36,470:************************* INFO(139987148605248)2022-07-07 16:14:36,470:deblurring started WARNING(139987148605248)2022-07-07 16:14:36,470:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data WARNING(139987148605248)2022-07-07 16:14:36,470:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': (), 'neg_ref_fp': (), 'neg_ref_db_fp': (), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 8, 'is_worker_thread': False, 'logger': } INFO(139987148605248)2022-07-07 16:14:36,470:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139987148605248)2022-07-07 16:14:36,470:deblur main program started INFO(139987148605248)2022-07-07 16:14:36,470:processing directory /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data INFO(139987148605248)2022-07-07 16:14:36,471:building negative db sortmerna index files INFO(139987148605248)2022-07-07 16:14:36,471:build_index_sortmerna files ['/home/ubuntu/Software/miniconda3/envs/qiime2-2022.2/lib/python3.8/site-packages/deblur/support_files/artifacts.fa'] to dir /tmp/tmp_yz2xtet/deblur_working_dir INFO(139987148605248)2022-07-07 16:14:36,532:building positive db sortmerna index files INFO(139987148605248)2022-07-07 16:14:36,532:build_index_sortmerna files ['/home/ubuntu/Software/miniconda3/envs/qiime2-2022.2/lib/python3.8/site-packages/deblur/support_files/88_otus.fasta'] to dir /tmp/tmp_yz2xtet/deblur_working_dir INFO(139987148605248)2022-07-07 16:15:05,860:parallel processing per sample fasta files INFO(139987148605248)2022-07-07 16:15:05,860:parallel deblur started for 36 inputs INFO(139863851616064)2022-07-07 16:15:06,665:************************* INFO(139863851616064)2022-07-07 16:15:06,665:deblurring started WARNING(139863851616064)2022-07-07 16:15:06,665:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-10_9_L001_R1_001.fastq.gz WARNING(139863851616064)2022-07-07 16:15:06,666:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-10_9_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139863851616064)2022-07-07 16:15:06,666:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139863851616064)2022-07-07 16:15:06,666:deblur worker thread started INFO(139863851616064)2022-07-07 16:15:06,666:processing per sample fasta files INFO(139863851616064)2022-07-07 16:15:06,666:-------------------------------------------------------- INFO(139863851616064)2022-07-07 16:15:06,666:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-10_9_L001_R1_001.fastq.gz INFO(140058815760192)2022-07-07 16:15:06,675:************************* INFO(140058815760192)2022-07-07 16:15:06,675:deblurring started WARNING(140058815760192)2022-07-07 16:15:06,675:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-13_12_L001_R1_001.fastq.gz WARNING(140058815760192)2022-07-07 16:15:06,675:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-13_12_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140058815760192)2022-07-07 16:15:06,675:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140058815760192)2022-07-07 16:15:06,675:deblur worker thread started INFO(140058815760192)2022-07-07 16:15:06,675:processing per sample fasta files INFO(140058815760192)2022-07-07 16:15:06,675:-------------------------------------------------------- INFO(140058815760192)2022-07-07 16:15:06,675:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-13_12_L001_R1_001.fastq.gz INFO(140476423567168)2022-07-07 16:15:06,952:************************* INFO(139731653822272)2022-07-07 16:15:06,952:************************* INFO(140476423567168)2022-07-07 16:15:06,952:deblurring started INFO(139731653822272)2022-07-07 16:15:06,952:deblurring started WARNING(140476423567168)2022-07-07 16:15:06,952:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-23_22_L001_R1_001.fastq.gz WARNING(139731653822272)2022-07-07 16:15:06,952:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-04_3_L001_R1_001.fastq.gz WARNING(140476423567168)2022-07-07 16:15:06,953:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-23_22_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } WARNING(139731653822272)2022-07-07 16:15:06,953:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-04_3_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140476423567168)2022-07-07 16:15:06,953:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140476423567168)2022-07-07 16:15:06,953:deblur worker thread started INFO(139731653822272)2022-07-07 16:15:06,953:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140476423567168)2022-07-07 16:15:06,953:processing per sample fasta files INFO(139731653822272)2022-07-07 16:15:06,953:deblur worker thread started INFO(139731653822272)2022-07-07 16:15:06,953:processing per sample fasta files INFO(140476423567168)2022-07-07 16:15:06,953:-------------------------------------------------------- INFO(140476423567168)2022-07-07 16:15:06,953:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-23_22_L001_R1_001.fastq.gz INFO(139731653822272)2022-07-07 16:15:06,953:-------------------------------------------------------- INFO(139731653822272)2022-07-07 16:15:06,953:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-04_3_L001_R1_001.fastq.gz INFO(139781058893632)2022-07-07 16:15:06,979:************************* INFO(139781058893632)2022-07-07 16:15:06,979:deblurring started WARNING(139781058893632)2022-07-07 16:15:06,979:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-34_33_L001_R1_001.fastq.gz WARNING(139781058893632)2022-07-07 16:15:06,979:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-34_33_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139781058893632)2022-07-07 16:15:06,980:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139781058893632)2022-07-07 16:15:06,980:deblur worker thread started INFO(139781058893632)2022-07-07 16:15:06,980:processing per sample fasta files INFO(139781058893632)2022-07-07 16:15:06,980:-------------------------------------------------------- INFO(139781058893632)2022-07-07 16:15:06,980:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-34_33_L001_R1_001.fastq.gz INFO(140116986238784)2022-07-07 16:15:06,987:************************* INFO(140116986238784)2022-07-07 16:15:06,987:deblurring started WARNING(140116986238784)2022-07-07 16:15:06,988:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-31_30_L001_R1_001.fastq.gz WARNING(140116986238784)2022-07-07 16:15:06,988:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-31_30_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140116986238784)2022-07-07 16:15:06,988:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140116986238784)2022-07-07 16:15:06,988:deblur worker thread started INFO(140116986238784)2022-07-07 16:15:06,988:processing per sample fasta files INFO(140116986238784)2022-07-07 16:15:06,988:-------------------------------------------------------- INFO(140116986238784)2022-07-07 16:15:06,988:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-31_30_L001_R1_001.fastq.gz INFO(140472148764480)2022-07-07 16:15:06,993:************************* INFO(140472148764480)2022-07-07 16:15:06,993:deblurring started WARNING(140472148764480)2022-07-07 16:15:06,993:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-11_10_L001_R1_001.fastq.gz WARNING(140472148764480)2022-07-07 16:15:06,993:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-11_10_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140472148764480)2022-07-07 16:15:06,993:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140472148764480)2022-07-07 16:15:06,993:deblur worker thread started INFO(140472148764480)2022-07-07 16:15:06,993:processing per sample fasta files INFO(140472148764480)2022-07-07 16:15:06,993:-------------------------------------------------------- INFO(140472148764480)2022-07-07 16:15:06,993:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-11_10_L001_R1_001.fastq.gz INFO(140229051279168)2022-07-07 16:15:06,994:************************* INFO(140229051279168)2022-07-07 16:15:06,994:deblurring started WARNING(140229051279168)2022-07-07 16:15:06,994:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-30_29_L001_R1_001.fastq.gz WARNING(140229051279168)2022-07-07 16:15:06,994:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-30_29_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140229051279168)2022-07-07 16:15:06,994:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140229051279168)2022-07-07 16:15:06,994:deblur worker thread started INFO(140229051279168)2022-07-07 16:15:06,994:processing per sample fasta files INFO(140229051279168)2022-07-07 16:15:06,994:-------------------------------------------------------- INFO(140229051279168)2022-07-07 16:15:06,994:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-30_29_L001_R1_001.fastq.gz INFO(139781058893632)2022-07-07 16:46:44,563:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-34_33_L001_R1_001.fastq.gz.trim INFO(139781058893632)2022-07-07 16:47:04,902:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-34_33_L001_R1_001.fastq.gz.trim.derep INFO(139781058893632)2022-07-07 16:47:32,063:total sequences 626379, passing sequences 626379, failing sequences 0 INFO(139781058893632)2022-07-07 16:47:32,064:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-34_33_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139731653822272)2022-07-07 16:57:59,851:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-04_3_L001_R1_001.fastq.gz.trim INFO(139731653822272)2022-07-07 16:58:15,953:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-04_3_L001_R1_001.fastq.gz.trim.derep INFO(139731653822272)2022-07-07 16:58:42,553:total sequences 1007136, passing sequences 1007135, failing sequences 1 INFO(139731653822272)2022-07-07 16:58:42,553:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-04_3_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140476423567168)2022-07-07 17:00:15,646:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-23_22_L001_R1_001.fastq.gz.trim INFO(140476423567168)2022-07-07 17:00:41,805:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-23_22_L001_R1_001.fastq.gz.trim.derep INFO(140476423567168)2022-07-07 17:01:14,688:total sequences 782809, passing sequences 782809, failing sequences 0 INFO(140476423567168)2022-07-07 17:01:14,688:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-23_22_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140116986238784)2022-07-07 17:05:35,309:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim INFO(140116986238784)2022-07-07 17:06:04,080:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim.derep INFO(139863851616064)2022-07-07 17:06:16,364:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-10_9_L001_R1_001.fastq.gz.trim INFO(139863851616064)2022-07-07 17:06:42,910:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-10_9_L001_R1_001.fastq.gz.trim.derep INFO(140116986238784)2022-07-07 17:06:55,565:total sequences 1277990, passing sequences 1277990, failing sequences 0 INFO(140116986238784)2022-07-07 17:06:55,565:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139863851616064)2022-07-07 17:07:29,843:total sequences 1327746, passing sequences 1327744, failing sequences 2 INFO(139863851616064)2022-07-07 17:07:29,843:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-10_9_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140229051279168)2022-07-07 17:10:00,348:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-30_29_L001_R1_001.fastq.gz.trim INFO(140229051279168)2022-07-07 17:10:29,230:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-30_29_L001_R1_001.fastq.gz.trim.derep INFO(140229051279168)2022-07-07 17:11:11,138:total sequences 1167541, passing sequences 1167541, failing sequences 0 INFO(140229051279168)2022-07-07 17:11:11,138:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-30_29_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140058815760192)2022-07-07 17:15:09,522:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim INFO(140058815760192)2022-07-07 17:15:45,556:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim.derep INFO(140472148764480)2022-07-07 17:16:38,037:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim INFO(140058815760192)2022-07-07 17:16:41,260:total sequences 1519158, passing sequences 1519158, failing sequences 0 INFO(140058815760192)2022-07-07 17:16:41,260:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140472148764480)2022-07-07 17:17:15,410:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim.derep INFO(140472148764480)2022-07-07 17:18:08,367:total sequences 1575108, passing sequences 1575108, failing sequences 0 INFO(140472148764480)2022-07-07 17:18:08,367:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139781058893632)2022-07-07 19:15:06,693:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-34_33_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139781058893632)2022-07-07 19:15:07,514:msa failed. aborting WARNING(139781058893632)2022-07-07 19:15:07,514:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-34_33_L001_R1_001.fastq.gz INFO(139781058893632)2022-07-07 19:15:07,514:finished processing per sample fasta files INFO(139781058893632)2022-07-07 19:15:07,514:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-34_33_L001_R1_001.fastq.gz finished INFO(140709137512256)2022-07-07 19:15:27,221:************************* INFO(140709137512256)2022-07-07 19:15:27,221:deblurring started WARNING(140709137512256)2022-07-07 19:15:27,221:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-24_23_L001_R1_001.fastq.gz WARNING(140709137512256)2022-07-07 19:15:27,221:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-24_23_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140709137512256)2022-07-07 19:15:27,221:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140709137512256)2022-07-07 19:15:27,221:deblur worker thread started INFO(140709137512256)2022-07-07 19:15:27,221:processing per sample fasta files INFO(140709137512256)2022-07-07 19:15:27,221:-------------------------------------------------------- INFO(140709137512256)2022-07-07 19:15:27,221:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-24_23_L001_R1_001.fastq.gz INFO(140709137512256)2022-07-07 20:11:37,417:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-24_23_L001_R1_001.fastq.gz.trim INFO(140476423567168)2022-07-07 20:11:44,972:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-23_22_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140476423567168)2022-07-07 20:11:46,832:msa failed. aborting WARNING(140476423567168)2022-07-07 20:11:46,835:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-23_22_L001_R1_001.fastq.gz INFO(140476423567168)2022-07-07 20:11:46,835:finished processing per sample fasta files INFO(140476423567168)2022-07-07 20:11:46,835:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-23_22_L001_R1_001.fastq.gz finished INFO(139892731242304)2022-07-07 20:12:24,138:************************* INFO(139892731242304)2022-07-07 20:12:24,138:deblurring started WARNING(139892731242304)2022-07-07 20:12:24,138:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-21_20_L001_R1_001.fastq.gz WARNING(139892731242304)2022-07-07 20:12:24,138:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-21_20_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139892731242304)2022-07-07 20:12:24,138:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139892731242304)2022-07-07 20:12:24,138:deblur worker thread started INFO(139892731242304)2022-07-07 20:12:24,138:processing per sample fasta files INFO(139892731242304)2022-07-07 20:12:24,138:-------------------------------------------------------- INFO(139892731242304)2022-07-07 20:12:24,138:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-21_20_L001_R1_001.fastq.gz INFO(140709137512256)2022-07-07 20:13:05,696:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-24_23_L001_R1_001.fastq.gz.trim.derep INFO(140709137512256)2022-07-07 20:13:50,810:total sequences 1214700, passing sequences 1214700, failing sequences 0 INFO(140709137512256)2022-07-07 20:13:50,810:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-24_23_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139863851616064)2022-07-07 20:40:52,579:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-10_9_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139863851616064)2022-07-07 20:40:53,023:msa failed. aborting WARNING(139863851616064)2022-07-07 20:40:53,024:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-10_9_L001_R1_001.fastq.gz INFO(139863851616064)2022-07-07 20:40:53,024:finished processing per sample fasta files INFO(139863851616064)2022-07-07 20:40:53,024:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-10_9_L001_R1_001.fastq.gz finished INFO(139794542896960)2022-07-07 20:41:13,836:************************* INFO(139794542896960)2022-07-07 20:41:13,836:deblurring started WARNING(139794542896960)2022-07-07 20:41:13,836:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-29_28_L001_R1_001.fastq.gz WARNING(139794542896960)2022-07-07 20:41:13,836:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-29_28_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139794542896960)2022-07-07 20:41:13,837:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139794542896960)2022-07-07 20:41:13,837:deblur worker thread started INFO(139794542896960)2022-07-07 20:41:13,837:processing per sample fasta files INFO(139794542896960)2022-07-07 20:41:13,837:-------------------------------------------------------- INFO(139794542896960)2022-07-07 20:41:13,837:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-29_28_L001_R1_001.fastq.gz INFO(139731653822272)2022-07-07 20:49:13,908:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-04_3_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139731653822272)2022-07-07 20:49:15,032:msa failed. aborting WARNING(139731653822272)2022-07-07 20:49:15,078:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-04_3_L001_R1_001.fastq.gz INFO(139731653822272)2022-07-07 20:49:15,078:finished processing per sample fasta files INFO(139731653822272)2022-07-07 20:49:15,078:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-04_3_L001_R1_001.fastq.gz finished INFO(139664027010880)2022-07-07 20:49:36,570:************************* INFO(139664027010880)2022-07-07 20:49:36,571:deblurring started WARNING(139664027010880)2022-07-07 20:49:36,571:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-35_34_L001_R1_001.fastq.gz WARNING(139664027010880)2022-07-07 20:49:36,571:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-35_34_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139664027010880)2022-07-07 20:49:36,571:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139664027010880)2022-07-07 20:49:36,571:deblur worker thread started INFO(139664027010880)2022-07-07 20:49:36,571:processing per sample fasta files INFO(139664027010880)2022-07-07 20:49:36,571:-------------------------------------------------------- INFO(139664027010880)2022-07-07 20:49:36,571:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-35_34_L001_R1_001.fastq.gz INFO(139892731242304)2022-07-07 21:11:34,075:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-21_20_L001_R1_001.fastq.gz.trim INFO(139892731242304)2022-07-07 21:12:24,688:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-21_20_L001_R1_001.fastq.gz.trim.derep INFO(139892731242304)2022-07-07 21:13:23,901:total sequences 1577463, passing sequences 1577463, failing sequences 0 INFO(139892731242304)2022-07-07 21:13:23,901:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-21_20_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139794542896960)2022-07-07 21:28:28,182:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-29_28_L001_R1_001.fastq.gz.trim INFO(139794542896960)2022-07-07 21:28:55,993:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-29_28_L001_R1_001.fastq.gz.trim.derep INFO(139794542896960)2022-07-07 21:29:43,472:total sequences 1319875, passing sequences 1319875, failing sequences 0 INFO(139794542896960)2022-07-07 21:29:43,472:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-29_28_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139664027010880)2022-07-07 22:10:09,946:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-35_34_L001_R1_001.fastq.gz.trim INFO(140472148764480)2022-07-07 22:11:28,208:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) INFO(139664027010880)2022-07-07 22:11:29,690:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-35_34_L001_R1_001.fastq.gz.trim.derep WARNING(140472148764480)2022-07-07 22:11:30,359:msa failed. aborting WARNING(140472148764480)2022-07-07 22:11:30,359:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-11_10_L001_R1_001.fastq.gz INFO(140472148764480)2022-07-07 22:11:30,359:finished processing per sample fasta files INFO(140472148764480)2022-07-07 22:11:30,359:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-11_10_L001_R1_001.fastq.gz finished INFO(140583031539520)2022-07-07 22:11:54,085:************************* INFO(140583031539520)2022-07-07 22:11:54,086:deblurring started WARNING(140583031539520)2022-07-07 22:11:54,086:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-33_32_L001_R1_001.fastq.gz WARNING(140583031539520)2022-07-07 22:11:54,086:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-33_32_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140583031539520)2022-07-07 22:11:54,086:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140583031539520)2022-07-07 22:11:54,086:deblur worker thread started INFO(140583031539520)2022-07-07 22:11:54,086:processing per sample fasta files INFO(140583031539520)2022-07-07 22:11:54,086:-------------------------------------------------------- INFO(140583031539520)2022-07-07 22:11:54,086:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-33_32_L001_R1_001.fastq.gz INFO(139664027010880)2022-07-07 22:12:24,127:total sequences 1531538, passing sequences 1531538, failing sequences 0 INFO(139664027010880)2022-07-07 22:12:24,127:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-35_34_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140116986238784)2022-07-07 22:17:59,023:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140116986238784)2022-07-07 22:17:59,702:msa failed. aborting WARNING(140116986238784)2022-07-07 22:17:59,702:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-31_30_L001_R1_001.fastq.gz INFO(140116986238784)2022-07-07 22:17:59,702:finished processing per sample fasta files INFO(140116986238784)2022-07-07 22:17:59,702:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-31_30_L001_R1_001.fastq.gz finished INFO(139626868979520)2022-07-07 22:18:21,748:************************* INFO(139626868979520)2022-07-07 22:18:21,748:deblurring started WARNING(139626868979520)2022-07-07 22:18:21,749:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-01_0_L001_R1_001.fastq.gz WARNING(139626868979520)2022-07-07 22:18:21,749:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-01_0_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139626868979520)2022-07-07 22:18:21,749:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139626868979520)2022-07-07 22:18:21,749:deblur worker thread started INFO(139626868979520)2022-07-07 22:18:21,749:processing per sample fasta files INFO(139626868979520)2022-07-07 22:18:21,749:-------------------------------------------------------- INFO(139626868979520)2022-07-07 22:18:21,749:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-01_0_L001_R1_001.fastq.gz INFO(140229051279168)2022-07-07 22:30:15,632:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-30_29_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140229051279168)2022-07-07 22:30:17,105:msa failed. aborting WARNING(140229051279168)2022-07-07 22:30:17,164:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-30_29_L001_R1_001.fastq.gz INFO(140229051279168)2022-07-07 22:30:17,164:finished processing per sample fasta files INFO(140229051279168)2022-07-07 22:30:17,164:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-30_29_L001_R1_001.fastq.gz finished INFO(140305306642240)2022-07-07 22:30:39,516:************************* INFO(140305306642240)2022-07-07 22:30:39,516:deblurring started WARNING(140305306642240)2022-07-07 22:30:39,516:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-28_27_L001_R1_001.fastq.gz WARNING(140305306642240)2022-07-07 22:30:39,516:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-28_27_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140305306642240)2022-07-07 22:30:39,516:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140305306642240)2022-07-07 22:30:39,516:deblur worker thread started INFO(140305306642240)2022-07-07 22:30:39,516:processing per sample fasta files INFO(140305306642240)2022-07-07 22:30:39,517:-------------------------------------------------------- INFO(140305306642240)2022-07-07 22:30:39,517:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-28_27_L001_R1_001.fastq.gz INFO(140305306642240)2022-07-07 23:03:21,394:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-28_27_L001_R1_001.fastq.gz.trim INFO(140305306642240)2022-07-07 23:03:39,479:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-28_27_L001_R1_001.fastq.gz.trim.derep INFO(140305306642240)2022-07-07 23:04:09,131:total sequences 812894, passing sequences 812894, failing sequences 0 INFO(140305306642240)2022-07-07 23:04:09,131:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-28_27_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139626868979520)2022-07-07 23:16:32,710:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim INFO(139626868979520)2022-07-07 23:17:12,666:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim.derep INFO(139626868979520)2022-07-07 23:17:56,629:total sequences 1200846, passing sequences 1200846, failing sequences 0 INFO(139626868979520)2022-07-07 23:17:56,629:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140583031539520)2022-07-07 23:22:40,707:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim INFO(140583031539520)2022-07-07 23:23:28,512:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim.derep INFO(140583031539520)2022-07-07 23:24:35,808:total sequences 1834312, passing sequences 1834312, failing sequences 0 INFO(140583031539520)2022-07-07 23:24:35,809:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140058815760192)2022-07-08 00:05:14,134:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140058815760192)2022-07-08 00:05:15,480:msa failed. aborting WARNING(140058815760192)2022-07-08 00:05:15,490:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-13_12_L001_R1_001.fastq.gz INFO(140058815760192)2022-07-08 00:05:15,490:finished processing per sample fasta files INFO(140058815760192)2022-07-08 00:05:15,490:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-13_12_L001_R1_001.fastq.gz finished INFO(139798731749184)2022-07-08 00:05:36,297:************************* INFO(139798731749184)2022-07-08 00:05:36,297:deblurring started WARNING(139798731749184)2022-07-08 00:05:36,297:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-09_8_L001_R1_001.fastq.gz WARNING(139798731749184)2022-07-08 00:05:36,297:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-09_8_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139798731749184)2022-07-08 00:05:36,297:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139798731749184)2022-07-08 00:05:36,298:deblur worker thread started INFO(139798731749184)2022-07-08 00:05:36,298:processing per sample fasta files INFO(139798731749184)2022-07-08 00:05:36,298:-------------------------------------------------------- INFO(139798731749184)2022-07-08 00:05:36,298:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-09_8_L001_R1_001.fastq.gz INFO(139798731749184)2022-07-08 00:57:25,407:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim INFO(139798731749184)2022-07-08 00:57:44,155:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim.derep INFO(139798731749184)2022-07-08 00:58:18,113:total sequences 1393401, passing sequences 1393401, failing sequences 0 INFO(139798731749184)2022-07-08 00:58:18,113:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140709137512256)2022-07-08 01:04:21,661:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-24_23_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140709137512256)2022-07-08 01:04:22,105:msa failed. aborting WARNING(140709137512256)2022-07-08 01:04:22,106:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-24_23_L001_R1_001.fastq.gz INFO(140709137512256)2022-07-08 01:04:22,106:finished processing per sample fasta files INFO(140709137512256)2022-07-08 01:04:22,106:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-24_23_L001_R1_001.fastq.gz finished INFO(139722544256832)2022-07-08 01:04:42,257:************************* INFO(139722544256832)2022-07-08 01:04:42,257:deblurring started WARNING(139722544256832)2022-07-08 01:04:42,257:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-05_4_L001_R1_001.fastq.gz WARNING(139722544256832)2022-07-08 01:04:42,257:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-05_4_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139722544256832)2022-07-08 01:04:42,257:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139722544256832)2022-07-08 01:04:42,257:deblur worker thread started INFO(139722544256832)2022-07-08 01:04:42,257:processing per sample fasta files INFO(139722544256832)2022-07-08 01:04:42,257:-------------------------------------------------------- INFO(139722544256832)2022-07-08 01:04:42,257:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-05_4_L001_R1_001.fastq.gz INFO(139892731242304)2022-07-08 01:50:20,419:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-21_20_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139892731242304)2022-07-08 01:50:21,349:msa failed. aborting WARNING(139892731242304)2022-07-08 01:50:21,349:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-21_20_L001_R1_001.fastq.gz INFO(139892731242304)2022-07-08 01:50:21,349:finished processing per sample fasta files INFO(139892731242304)2022-07-08 01:50:21,349:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-21_20_L001_R1_001.fastq.gz finished INFO(139669948643136)2022-07-08 01:50:41,330:************************* INFO(139669948643136)2022-07-08 01:50:41,330:deblurring started WARNING(139669948643136)2022-07-08 01:50:41,330:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-02_1_L001_R1_001.fastq.gz WARNING(139669948643136)2022-07-08 01:50:41,331:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-02_1_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139669948643136)2022-07-08 01:50:41,331:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139669948643136)2022-07-08 01:50:41,331:deblur worker thread started INFO(139669948643136)2022-07-08 01:50:41,331:processing per sample fasta files INFO(139669948643136)2022-07-08 01:50:41,331:-------------------------------------------------------- INFO(139669948643136)2022-07-08 01:50:41,331:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-02_1_L001_R1_001.fastq.gz INFO(139722544256832)2022-07-08 02:09:58,582:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-05_4_L001_R1_001.fastq.gz.trim INFO(139722544256832)2022-07-08 02:10:56,808:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-05_4_L001_R1_001.fastq.gz.trim.derep INFO(139722544256832)2022-07-08 02:11:59,684:total sequences 1556632, passing sequences 1556632, failing sequences 0 INFO(139722544256832)2022-07-08 02:11:59,684:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-05_4_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139794542896960)2022-07-08 02:19:24,944:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-29_28_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139794542896960)2022-07-08 02:19:26,129:msa failed. aborting WARNING(139794542896960)2022-07-08 02:19:26,129:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-29_28_L001_R1_001.fastq.gz INFO(139794542896960)2022-07-08 02:19:26,129:finished processing per sample fasta files INFO(139794542896960)2022-07-08 02:19:26,129:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-29_28_L001_R1_001.fastq.gz finished INFO(140601095825216)2022-07-08 02:19:44,242:************************* INFO(140601095825216)2022-07-08 02:19:44,243:deblurring started WARNING(140601095825216)2022-07-08 02:19:44,243:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-25_24_L001_R1_001.fastq.gz WARNING(140601095825216)2022-07-08 02:19:44,243:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-25_24_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140601095825216)2022-07-08 02:19:44,243:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140601095825216)2022-07-08 02:19:44,243:deblur worker thread started INFO(140601095825216)2022-07-08 02:19:44,243:processing per sample fasta files INFO(140601095825216)2022-07-08 02:19:44,243:-------------------------------------------------------- INFO(140601095825216)2022-07-08 02:19:44,243:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-25_24_L001_R1_001.fastq.gz INFO(140305306642240)2022-07-08 02:34:09,292:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-28_27_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140305306642240)2022-07-08 02:34:09,571:msa failed. aborting WARNING(140305306642240)2022-07-08 02:34:09,571:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-28_27_L001_R1_001.fastq.gz INFO(140305306642240)2022-07-08 02:34:09,571:finished processing per sample fasta files INFO(140305306642240)2022-07-08 02:34:09,571:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-28_27_L001_R1_001.fastq.gz finished INFO(140160771610432)2022-07-08 02:34:28,933:************************* INFO(140160771610432)2022-07-08 02:34:28,933:deblurring started WARNING(140160771610432)2022-07-08 02:34:28,933:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-18_17_L001_R1_001.fastq.gz WARNING(140160771610432)2022-07-08 02:34:28,933:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-18_17_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140160771610432)2022-07-08 02:34:28,933:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140160771610432)2022-07-08 02:34:28,933:deblur worker thread started INFO(140160771610432)2022-07-08 02:34:28,934:processing per sample fasta files INFO(140160771610432)2022-07-08 02:34:28,934:-------------------------------------------------------- INFO(140160771610432)2022-07-08 02:34:28,934:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-18_17_L001_R1_001.fastq.gz INFO(139669948643136)2022-07-08 02:53:25,877:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-02_1_L001_R1_001.fastq.gz.trim INFO(139669948643136)2022-07-08 02:54:11,897:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-02_1_L001_R1_001.fastq.gz.trim.derep INFO(139669948643136)2022-07-08 02:54:58,945:total sequences 1492100, passing sequences 1492100, failing sequences 0 INFO(139669948643136)2022-07-08 02:54:58,945:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-02_1_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140583031539520)2022-07-08 03:06:37,513:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140583031539520)2022-07-08 03:06:37,825:msa failed. aborting WARNING(140583031539520)2022-07-08 03:06:37,825:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-33_32_L001_R1_001.fastq.gz INFO(140583031539520)2022-07-08 03:06:37,825:finished processing per sample fasta files INFO(140583031539520)2022-07-08 03:06:37,825:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-33_32_L001_R1_001.fastq.gz finished INFO(140489964242752)2022-07-08 03:06:52,953:************************* INFO(140489964242752)2022-07-08 03:06:52,954:deblurring started WARNING(140489964242752)2022-07-08 03:06:52,954:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-12_11_L001_R1_001.fastq.gz WARNING(140489964242752)2022-07-08 03:06:52,954:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-12_11_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140489964242752)2022-07-08 03:06:52,954:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140489964242752)2022-07-08 03:06:52,954:deblur worker thread started INFO(140489964242752)2022-07-08 03:06:52,954:processing per sample fasta files INFO(140489964242752)2022-07-08 03:06:52,954:-------------------------------------------------------- INFO(140489964242752)2022-07-08 03:06:52,954:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-12_11_L001_R1_001.fastq.gz INFO(140601095825216)2022-07-08 03:17:20,095:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-25_24_L001_R1_001.fastq.gz.trim INFO(140601095825216)2022-07-08 03:18:06,865:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-25_24_L001_R1_001.fastq.gz.trim.derep INFO(140601095825216)2022-07-08 03:18:53,299:total sequences 1319603, passing sequences 1319603, failing sequences 0 INFO(140601095825216)2022-07-08 03:18:53,299:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-25_24_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140160771610432)2022-07-08 03:41:22,946:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-18_17_L001_R1_001.fastq.gz.trim INFO(140160771610432)2022-07-08 03:42:08,275:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-18_17_L001_R1_001.fastq.gz.trim.derep INFO(140160771610432)2022-07-08 03:43:20,855:total sequences 1748380, passing sequences 1748380, failing sequences 0 INFO(140160771610432)2022-07-08 03:43:20,855:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-18_17_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140489964242752)2022-07-08 04:03:02,381:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-12_11_L001_R1_001.fastq.gz.trim INFO(140489964242752)2022-07-08 04:03:41,465:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-12_11_L001_R1_001.fastq.gz.trim.derep INFO(140489964242752)2022-07-08 04:04:29,370:total sequences 1331919, passing sequences 1331919, failing sequences 0 INFO(140489964242752)2022-07-08 04:04:29,370:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-12_11_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139664027010880)2022-07-08 04:10:55,290:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-35_34_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139664027010880)2022-07-08 04:10:55,921:msa failed. aborting WARNING(139664027010880)2022-07-08 04:10:55,921:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-35_34_L001_R1_001.fastq.gz INFO(139664027010880)2022-07-08 04:10:55,921:finished processing per sample fasta files INFO(139664027010880)2022-07-08 04:10:55,921:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-35_34_L001_R1_001.fastq.gz finished INFO(140375054292800)2022-07-08 04:11:13,243:************************* INFO(140375054292800)2022-07-08 04:11:13,243:deblurring started WARNING(140375054292800)2022-07-08 04:11:13,243:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-14_13_L001_R1_001.fastq.gz WARNING(140375054292800)2022-07-08 04:11:13,243:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-14_13_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140375054292800)2022-07-08 04:11:13,243:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140375054292800)2022-07-08 04:11:13,243:deblur worker thread started INFO(140375054292800)2022-07-08 04:11:13,243:processing per sample fasta files INFO(140375054292800)2022-07-08 04:11:13,243:-------------------------------------------------------- INFO(140375054292800)2022-07-08 04:11:13,243:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-14_13_L001_R1_001.fastq.gz INFO(139626868979520)2022-07-08 04:20:57,084:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139626868979520)2022-07-08 04:20:58,070:msa failed. aborting WARNING(139626868979520)2022-07-08 04:20:58,070:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-01_0_L001_R1_001.fastq.gz INFO(139626868979520)2022-07-08 04:20:58,071:finished processing per sample fasta files INFO(139626868979520)2022-07-08 04:20:58,071:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-01_0_L001_R1_001.fastq.gz finished INFO(140268624230208)2022-07-08 04:21:13,498:************************* INFO(140268624230208)2022-07-08 04:21:13,498:deblurring started WARNING(140268624230208)2022-07-08 04:21:13,498:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-16_15_L001_R1_001.fastq.gz WARNING(140268624230208)2022-07-08 04:21:13,498:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-16_15_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140268624230208)2022-07-08 04:21:13,498:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140268624230208)2022-07-08 04:21:13,498:deblur worker thread started INFO(140268624230208)2022-07-08 04:21:13,498:processing per sample fasta files INFO(140268624230208)2022-07-08 04:21:13,498:-------------------------------------------------------- INFO(140268624230208)2022-07-08 04:21:13,498:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-16_15_L001_R1_001.fastq.gz INFO(140375054292800)2022-07-08 05:12:22,327:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-14_13_L001_R1_001.fastq.gz.trim INFO(140375054292800)2022-07-08 05:12:59,482:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-14_13_L001_R1_001.fastq.gz.trim.derep INFO(140268624230208)2022-07-08 05:13:35,377:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-16_15_L001_R1_001.fastq.gz.trim INFO(140375054292800)2022-07-08 05:13:59,144:total sequences 1575569, passing sequences 1575569, failing sequences 0 INFO(140375054292800)2022-07-08 05:13:59,144:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-14_13_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140268624230208)2022-07-08 05:14:03,403:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-16_15_L001_R1_001.fastq.gz.trim.derep INFO(140268624230208)2022-07-08 05:14:50,277:total sequences 1197336, passing sequences 1197334, failing sequences 2 INFO(140268624230208)2022-07-08 05:14:50,277:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-16_15_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139722544256832)2022-07-08 05:26:31,764:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-05_4_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139722544256832)2022-07-08 05:26:32,241:msa failed. aborting WARNING(139722544256832)2022-07-08 05:26:32,242:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-05_4_L001_R1_001.fastq.gz INFO(139722544256832)2022-07-08 05:26:32,242:finished processing per sample fasta files INFO(139722544256832)2022-07-08 05:26:32,242:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-05_4_L001_R1_001.fastq.gz finished INFO(139900063876928)2022-07-08 05:26:46,269:************************* INFO(139900063876928)2022-07-08 05:26:46,269:deblurring started WARNING(139900063876928)2022-07-08 05:26:46,269:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-36_35_L001_R1_001.fastq.gz WARNING(139900063876928)2022-07-08 05:26:46,270:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-36_35_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139900063876928)2022-07-08 05:26:46,270:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139900063876928)2022-07-08 05:26:46,270:deblur worker thread started INFO(139900063876928)2022-07-08 05:26:46,270:processing per sample fasta files INFO(139900063876928)2022-07-08 05:26:46,270:-------------------------------------------------------- INFO(139900063876928)2022-07-08 05:26:46,270:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-36_35_L001_R1_001.fastq.gz INFO(140601095825216)2022-07-08 05:52:32,527:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-25_24_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140601095825216)2022-07-08 05:52:32,853:msa failed. aborting WARNING(140601095825216)2022-07-08 05:52:32,853:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-25_24_L001_R1_001.fastq.gz INFO(140601095825216)2022-07-08 05:52:32,853:finished processing per sample fasta files INFO(140601095825216)2022-07-08 05:52:32,853:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-25_24_L001_R1_001.fastq.gz finished INFO(139705194006336)2022-07-08 05:52:45,988:************************* INFO(139705194006336)2022-07-08 05:52:45,989:deblurring started WARNING(139705194006336)2022-07-08 05:52:45,989:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-27_26_L001_R1_001.fastq.gz WARNING(139705194006336)2022-07-08 05:52:45,989:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-27_26_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139705194006336)2022-07-08 05:52:45,989:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139705194006336)2022-07-08 05:52:45,989:deblur worker thread started INFO(139705194006336)2022-07-08 05:52:45,989:processing per sample fasta files INFO(139705194006336)2022-07-08 05:52:45,989:-------------------------------------------------------- INFO(139705194006336)2022-07-08 05:52:45,989:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-27_26_L001_R1_001.fastq.gz INFO(139900063876928)2022-07-08 06:09:34,260:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-36_35_L001_R1_001.fastq.gz.trim INFO(140160771610432)2022-07-08 06:14:02,856:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-18_17_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140160771610432)2022-07-08 06:14:03,400:msa failed. aborting WARNING(140160771610432)2022-07-08 06:14:03,400:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-18_17_L001_R1_001.fastq.gz INFO(140160771610432)2022-07-08 06:14:03,400:finished processing per sample fasta files INFO(140160771610432)2022-07-08 06:14:03,400:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-18_17_L001_R1_001.fastq.gz finished INFO(139900063876928)2022-07-08 06:14:07,193:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-36_35_L001_R1_001.fastq.gz.trim.derep INFO(140242557867840)2022-07-08 06:14:28,047:************************* INFO(140242557867840)2022-07-08 06:14:28,047:deblurring started WARNING(140242557867840)2022-07-08 06:14:28,047:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-20_19_L001_R1_001.fastq.gz WARNING(140242557867840)2022-07-08 06:14:28,047:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-20_19_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140242557867840)2022-07-08 06:14:28,047:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140242557867840)2022-07-08 06:14:28,047:deblur worker thread started INFO(140242557867840)2022-07-08 06:14:28,047:processing per sample fasta files INFO(140242557867840)2022-07-08 06:14:28,047:-------------------------------------------------------- INFO(140242557867840)2022-07-08 06:14:28,047:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-20_19_L001_R1_001.fastq.gz INFO(139900063876928)2022-07-08 06:14:34,119:total sequences 679422, passing sequences 679409, failing sequences 13 INFO(139900063876928)2022-07-08 06:14:34,119:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-36_35_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139798731749184)2022-07-08 06:42:20,159:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139798731749184)2022-07-08 06:42:20,482:msa failed. aborting WARNING(139798731749184)2022-07-08 06:42:20,482:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-09_8_L001_R1_001.fastq.gz INFO(139798731749184)2022-07-08 06:42:20,482:finished processing per sample fasta files INFO(139798731749184)2022-07-08 06:42:20,482:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-09_8_L001_R1_001.fastq.gz finished INFO(140403482928960)2022-07-08 06:42:34,129:************************* INFO(140403482928960)2022-07-08 06:42:34,129:deblurring started WARNING(140403482928960)2022-07-08 06:42:34,129:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-03_2_L001_R1_001.fastq.gz WARNING(140403482928960)2022-07-08 06:42:34,129:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-03_2_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140403482928960)2022-07-08 06:42:34,129:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140403482928960)2022-07-08 06:42:34,130:deblur worker thread started INFO(140403482928960)2022-07-08 06:42:34,130:processing per sample fasta files INFO(140403482928960)2022-07-08 06:42:34,130:-------------------------------------------------------- INFO(140403482928960)2022-07-08 06:42:34,130:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-03_2_L001_R1_001.fastq.gz INFO(139705194006336)2022-07-08 06:45:47,704:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-27_26_L001_R1_001.fastq.gz.trim INFO(139705194006336)2022-07-08 06:46:25,174:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-27_26_L001_R1_001.fastq.gz.trim.derep INFO(139705194006336)2022-07-08 06:47:00,525:total sequences 971514, passing sequences 971514, failing sequences 0 INFO(139705194006336)2022-07-08 06:47:00,525:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-27_26_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140242557867840)2022-07-08 06:53:45,320:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-20_19_L001_R1_001.fastq.gz.trim INFO(140242557867840)2022-07-08 06:54:15,367:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-20_19_L001_R1_001.fastq.gz.trim.derep INFO(140242557867840)2022-07-08 06:54:46,971:total sequences 901993, passing sequences 901993, failing sequences 0 INFO(140242557867840)2022-07-08 06:54:46,971:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-20_19_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140403482928960)2022-07-08 07:29:35,365:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-03_2_L001_R1_001.fastq.gz.trim INFO(140403482928960)2022-07-08 07:29:59,154:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-03_2_L001_R1_001.fastq.gz.trim.derep INFO(140403482928960)2022-07-08 07:30:35,428:total sequences 1058129, passing sequences 1058126, failing sequences 3 INFO(140403482928960)2022-07-08 07:30:35,429:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-03_2_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140375054292800)2022-07-08 08:17:33,952:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-14_13_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140375054292800)2022-07-08 08:17:34,862:msa failed. aborting WARNING(140375054292800)2022-07-08 08:17:34,862:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-14_13_L001_R1_001.fastq.gz INFO(140375054292800)2022-07-08 08:17:34,862:finished processing per sample fasta files INFO(140375054292800)2022-07-08 08:17:34,862:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-14_13_L001_R1_001.fastq.gz finished INFO(139650494113600)2022-07-08 08:17:48,379:************************* INFO(139650494113600)2022-07-08 08:17:48,379:deblurring started WARNING(139650494113600)2022-07-08 08:17:48,379:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-26_25_L001_R1_001.fastq.gz WARNING(139650494113600)2022-07-08 08:17:48,379:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-26_25_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139650494113600)2022-07-08 08:17:48,379:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139650494113600)2022-07-08 08:17:48,379:deblur worker thread started INFO(139650494113600)2022-07-08 08:17:48,379:processing per sample fasta files INFO(139650494113600)2022-07-08 08:17:48,379:-------------------------------------------------------- INFO(139650494113600)2022-07-08 08:17:48,380:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-26_25_L001_R1_001.fastq.gz INFO(139669948643136)2022-07-08 08:51:45,482:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-02_1_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139669948643136)2022-07-08 08:51:46,701:msa failed. aborting WARNING(139669948643136)2022-07-08 08:51:46,701:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-02_1_L001_R1_001.fastq.gz INFO(139669948643136)2022-07-08 08:51:46,701:finished processing per sample fasta files INFO(139669948643136)2022-07-08 08:51:46,701:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-02_1_L001_R1_001.fastq.gz finished INFO(139809728182080)2022-07-08 08:52:08,683:************************* INFO(139809728182080)2022-07-08 08:52:08,683:deblurring started WARNING(139809728182080)2022-07-08 08:52:08,683:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-08_7_L001_R1_001.fastq.gz WARNING(139809728182080)2022-07-08 08:52:08,683:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-08_7_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139809728182080)2022-07-08 08:52:08,683:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139809728182080)2022-07-08 08:52:08,683:deblur worker thread started INFO(139809728182080)2022-07-08 08:52:08,683:processing per sample fasta files INFO(139809728182080)2022-07-08 08:52:08,683:-------------------------------------------------------- INFO(139809728182080)2022-07-08 08:52:08,683:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-08_7_L001_R1_001.fastq.gz INFO(139900063876928)2022-07-08 09:21:01,359:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-36_35_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139900063876928)2022-07-08 09:21:01,932:msa failed. aborting WARNING(139900063876928)2022-07-08 09:21:01,932:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-36_35_L001_R1_001.fastq.gz INFO(139900063876928)2022-07-08 09:21:01,933:finished processing per sample fasta files INFO(139900063876928)2022-07-08 09:21:01,933:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-36_35_L001_R1_001.fastq.gz finished INFO(140350464132928)2022-07-08 09:21:17,302:************************* INFO(140350464132928)2022-07-08 09:21:17,302:deblurring started WARNING(140350464132928)2022-07-08 09:21:17,302:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-06_5_L001_R1_001.fastq.gz WARNING(140350464132928)2022-07-08 09:21:17,302:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-06_5_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140350464132928)2022-07-08 09:21:17,302:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140350464132928)2022-07-08 09:21:17,302:deblur worker thread started INFO(140350464132928)2022-07-08 09:21:17,302:processing per sample fasta files INFO(140350464132928)2022-07-08 09:21:17,302:-------------------------------------------------------- INFO(140350464132928)2022-07-08 09:21:17,302:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-06_5_L001_R1_001.fastq.gz INFO(140489964242752)2022-07-08 09:34:15,544:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-12_11_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140489964242752)2022-07-08 09:34:16,045:msa failed. aborting WARNING(140489964242752)2022-07-08 09:34:16,045:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-12_11_L001_R1_001.fastq.gz INFO(140489964242752)2022-07-08 09:34:16,045:finished processing per sample fasta files INFO(140489964242752)2022-07-08 09:34:16,045:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-12_11_L001_R1_001.fastq.gz finished INFO(140479951865664)2022-07-08 09:34:28,498:************************* INFO(140479951865664)2022-07-08 09:34:28,498:deblurring started WARNING(140479951865664)2022-07-08 09:34:28,498:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-15_14_L001_R1_001.fastq.gz WARNING(140479951865664)2022-07-08 09:34:28,498:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-15_14_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140479951865664)2022-07-08 09:34:28,498:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140479951865664)2022-07-08 09:34:28,498:deblur worker thread started INFO(140479951865664)2022-07-08 09:34:28,498:processing per sample fasta files INFO(140479951865664)2022-07-08 09:34:28,498:-------------------------------------------------------- INFO(140479951865664)2022-07-08 09:34:28,498:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-15_14_L001_R1_001.fastq.gz INFO(139650494113600)2022-07-08 09:38:03,585:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-26_25_L001_R1_001.fastq.gz.trim INFO(139650494113600)2022-07-08 09:39:09,549:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-26_25_L001_R1_001.fastq.gz.trim.derep INFO(139650494113600)2022-07-08 09:39:50,709:total sequences 1541056, passing sequences 1541056, failing sequences 0 INFO(139650494113600)2022-07-08 09:39:50,709:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-26_25_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139809728182080)2022-07-08 10:03:27,899:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-08_7_L001_R1_001.fastq.gz.trim INFO(139809728182080)2022-07-08 10:04:32,068:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-08_7_L001_R1_001.fastq.gz.trim.derep INFO(139809728182080)2022-07-08 10:05:49,810:total sequences 1897628, passing sequences 1897628, failing sequences 0 INFO(139809728182080)2022-07-08 10:05:49,810:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-08_7_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140479951865664)2022-07-08 10:14:30,397:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-15_14_L001_R1_001.fastq.gz.trim INFO(140268624230208)2022-07-08 10:16:04,370:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-16_15_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140268624230208)2022-07-08 10:16:05,078:msa failed. aborting WARNING(140268624230208)2022-07-08 10:16:05,129:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-16_15_L001_R1_001.fastq.gz INFO(140268624230208)2022-07-08 10:16:05,129:finished processing per sample fasta files INFO(140268624230208)2022-07-08 10:16:05,129:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-16_15_L001_R1_001.fastq.gz finished INFO(139839098042176)2022-07-08 10:16:40,519:************************* INFO(139839098042176)2022-07-08 10:16:40,519:deblurring started WARNING(139839098042176)2022-07-08 10:16:40,519:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-19_18_L001_R1_001.fastq.gz WARNING(139839098042176)2022-07-08 10:16:40,519:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-19_18_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139839098042176)2022-07-08 10:16:40,519:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139839098042176)2022-07-08 10:16:40,519:deblur worker thread started INFO(139839098042176)2022-07-08 10:16:40,519:processing per sample fasta files INFO(139839098042176)2022-07-08 10:16:40,519:-------------------------------------------------------- INFO(139839098042176)2022-07-08 10:16:40,519:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-19_18_L001_R1_001.fastq.gz INFO(140479951865664)2022-07-08 10:17:15,884:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-15_14_L001_R1_001.fastq.gz.trim.derep INFO(140479951865664)2022-07-08 10:17:52,072:total sequences 1012700, passing sequences 1012700, failing sequences 0 INFO(140479951865664)2022-07-08 10:17:52,072:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-15_14_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140350464132928)2022-07-08 10:20:36,959:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-06_5_L001_R1_001.fastq.gz.trim INFO(140350464132928)2022-07-08 10:21:26,170:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-06_5_L001_R1_001.fastq.gz.trim.derep INFO(140350464132928)2022-07-08 10:22:13,833:total sequences 1183901, passing sequences 1183901, failing sequences 0 INFO(140350464132928)2022-07-08 10:22:13,833:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-06_5_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140242557867840)2022-07-08 10:59:27,263:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-20_19_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140242557867840)2022-07-08 10:59:28,405:msa failed. aborting WARNING(140242557867840)2022-07-08 10:59:28,405:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-20_19_L001_R1_001.fastq.gz INFO(140242557867840)2022-07-08 10:59:28,405:finished processing per sample fasta files INFO(140242557867840)2022-07-08 10:59:28,405:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-20_19_L001_R1_001.fastq.gz finished INFO(139936758077248)2022-07-08 10:59:46,068:************************* INFO(139936758077248)2022-07-08 10:59:46,068:deblurring started WARNING(139936758077248)2022-07-08 10:59:46,068:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-32_31_L001_R1_001.fastq.gz WARNING(139936758077248)2022-07-08 10:59:46,068:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-32_31_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139936758077248)2022-07-08 10:59:46,068:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139936758077248)2022-07-08 10:59:46,068:deblur worker thread started INFO(139936758077248)2022-07-08 10:59:46,068:processing per sample fasta files INFO(139936758077248)2022-07-08 10:59:46,068:-------------------------------------------------------- INFO(139936758077248)2022-07-08 10:59:46,068:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-32_31_L001_R1_001.fastq.gz INFO(139839098042176)2022-07-08 11:11:40,210:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-19_18_L001_R1_001.fastq.gz.trim INFO(139839098042176)2022-07-08 11:12:23,258:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-19_18_L001_R1_001.fastq.gz.trim.derep INFO(139839098042176)2022-07-08 11:13:23,926:total sequences 1452248, passing sequences 1452248, failing sequences 0 INFO(139839098042176)2022-07-08 11:13:23,926:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-19_18_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139705194006336)2022-07-08 11:28:03,480:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-27_26_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139705194006336)2022-07-08 11:28:04,446:msa failed. aborting WARNING(139705194006336)2022-07-08 11:28:04,447:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-27_26_L001_R1_001.fastq.gz INFO(139705194006336)2022-07-08 11:28:04,447:finished processing per sample fasta files INFO(139705194006336)2022-07-08 11:28:04,447:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-27_26_L001_R1_001.fastq.gz finished INFO(139936758077248)2022-07-08 11:57:31,057:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-32_31_L001_R1_001.fastq.gz.trim INFO(139936758077248)2022-07-08 11:58:11,623:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-32_31_L001_R1_001.fastq.gz.trim.derep INFO(139936758077248)2022-07-08 11:59:07,176:total sequences 1407036, passing sequences 1407036, failing sequences 0 INFO(139936758077248)2022-07-08 11:59:07,176:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-32_31_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140403482928960)2022-07-08 12:12:49,126:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-03_2_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140403482928960)2022-07-08 12:12:49,681:msa failed. aborting WARNING(140403482928960)2022-07-08 12:12:49,681:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-03_2_L001_R1_001.fastq.gz INFO(140403482928960)2022-07-08 12:12:49,681:finished processing per sample fasta files INFO(140403482928960)2022-07-08 12:12:49,682:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-03_2_L001_R1_001.fastq.gz finished INFO(140079994595136)2022-07-08 12:13:07,477:************************* INFO(140079994595136)2022-07-08 12:13:07,477:deblurring started WARNING(140079994595136)2022-07-08 12:13:07,477:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-22_21_L001_R1_001.fastq.gz WARNING(140079994595136)2022-07-08 12:13:07,477:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-22_21_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140079994595136)2022-07-08 12:13:07,477:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140079994595136)2022-07-08 12:13:07,477:deblur worker thread started INFO(140079994595136)2022-07-08 12:13:07,477:processing per sample fasta files INFO(140079994595136)2022-07-08 12:13:07,477:-------------------------------------------------------- INFO(140079994595136)2022-07-08 12:13:07,477:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-22_21_L001_R1_001.fastq.gz INFO(140079994595136)2022-07-08 13:17:36,315:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-22_21_L001_R1_001.fastq.gz.trim INFO(139650494113600)2022-07-08 13:43:22,161:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-26_25_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139650494113600)2022-07-08 13:43:24,004:msa failed. aborting WARNING(139650494113600)2022-07-08 13:43:24,004:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-26_25_L001_R1_001.fastq.gz INFO(139650494113600)2022-07-08 13:43:24,004:finished processing per sample fasta files INFO(139650494113600)2022-07-08 13:43:24,004:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-26_25_L001_R1_001.fastq.gz finished INFO(140079994595136)2022-07-08 13:44:36,693:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-22_21_L001_R1_001.fastq.gz.trim.derep INFO(140079994595136)2022-07-08 13:45:15,971:total sequences 1403252, passing sequences 1403252, failing sequences 0 INFO(140079994595136)2022-07-08 13:45:15,971:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-22_21_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139809728182080)2022-07-08 13:55:05,660:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-08_7_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139809728182080)2022-07-08 13:55:06,385:msa failed. aborting WARNING(139809728182080)2022-07-08 13:55:06,385:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-08_7_L001_R1_001.fastq.gz INFO(139809728182080)2022-07-08 13:55:06,385:finished processing per sample fasta files INFO(139809728182080)2022-07-08 13:55:06,386:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-08_7_L001_R1_001.fastq.gz finished INFO(140479951865664)2022-07-08 14:37:26,310:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-15_14_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140479951865664)2022-07-08 14:37:28,011:msa failed. aborting WARNING(140479951865664)2022-07-08 14:37:28,011:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-15_14_L001_R1_001.fastq.gz INFO(140479951865664)2022-07-08 14:37:28,011:finished processing per sample fasta files INFO(140479951865664)2022-07-08 14:37:28,011:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-15_14_L001_R1_001.fastq.gz finished INFO(140350464132928)2022-07-08 15:16:14,429:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-06_5_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140350464132928)2022-07-08 15:16:18,373:msa failed. aborting WARNING(140350464132928)2022-07-08 15:16:18,432:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-06_5_L001_R1_001.fastq.gz INFO(140350464132928)2022-07-08 15:16:18,441:finished processing per sample fasta files INFO(140350464132928)2022-07-08 15:16:18,441:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-06_5_L001_R1_001.fastq.gz finished INFO(140096826976064)2022-07-08 15:16:47,012:************************* INFO(140096826976064)2022-07-08 15:16:47,012:deblurring started WARNING(140096826976064)2022-07-08 15:16:47,012:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-07_6_L001_R1_001.fastq.gz WARNING(140096826976064)2022-07-08 15:16:47,012:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-07_6_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140096826976064)2022-07-08 15:16:47,012:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140096826976064)2022-07-08 15:16:47,012:deblur worker thread started INFO(140096826976064)2022-07-08 15:16:47,012:processing per sample fasta files INFO(140096826976064)2022-07-08 15:16:47,012:-------------------------------------------------------- INFO(140096826976064)2022-07-08 15:16:47,012:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-07_6_L001_R1_001.fastq.gz INFO(140096826976064)2022-07-08 16:02:10,227:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-07_6_L001_R1_001.fastq.gz.trim INFO(140096826976064)2022-07-08 16:02:35,104:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-07_6_L001_R1_001.fastq.gz.trim.derep INFO(140096826976064)2022-07-08 16:03:15,957:total sequences 1556273, passing sequences 1556273, failing sequences 0 INFO(140096826976064)2022-07-08 16:03:15,957:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-07_6_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139839098042176)2022-07-08 16:47:10,358:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-19_18_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139839098042176)2022-07-08 16:47:11,808:msa failed. aborting WARNING(139839098042176)2022-07-08 16:47:11,808:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-19_18_L001_R1_001.fastq.gz INFO(139839098042176)2022-07-08 16:47:11,808:finished processing per sample fasta files INFO(139839098042176)2022-07-08 16:47:11,808:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-19_18_L001_R1_001.fastq.gz finished INFO(139936758077248)2022-07-08 17:33:07,617:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-32_31_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(139936758077248)2022-07-08 17:33:09,987:msa failed. aborting WARNING(139936758077248)2022-07-08 17:33:10,073:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-32_31_L001_R1_001.fastq.gz INFO(139936758077248)2022-07-08 17:33:10,083:finished processing per sample fasta files INFO(139936758077248)2022-07-08 17:33:10,083:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-32_31_L001_R1_001.fastq.gz finished INFO(140019243595584)2022-07-08 17:33:29,349:************************* INFO(140019243595584)2022-07-08 17:33:29,349:deblurring started WARNING(140019243595584)2022-07-08 17:33:29,349:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-17_16_L001_R1_001.fastq.gz WARNING(140019243595584)2022-07-08 17:33:29,350:parameters: {'seqs_fp': '/tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-17_16_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp_yz2xtet', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp_yz2xtet/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 150, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140019243595584)2022-07-08 17:33:29,350:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140019243595584)2022-07-08 17:33:29,350:deblur worker thread started INFO(140019243595584)2022-07-08 17:33:29,350:processing per sample fasta files INFO(140019243595584)2022-07-08 17:33:29,350:-------------------------------------------------------- INFO(140019243595584)2022-07-08 17:33:29,350:launch_workflow for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-17_16_L001_R1_001.fastq.gz INFO(140019243595584)2022-07-08 18:18:01,318:dereplicate seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-17_16_L001_R1_001.fastq.gz.trim INFO(140019243595584)2022-07-08 18:18:24,533:remove_artifacts_seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-17_16_L001_R1_001.fastq.gz.trim.derep INFO(140019243595584)2022-07-08 18:19:02,089:total sequences 1569591, passing sequences 1569591, failing sequences 0 INFO(140019243595584)2022-07-08 18:19:02,089:multiple_sequence_alignment seqs file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-17_16_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140079994595136)2022-07-08 19:02:30,866:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-22_21_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140079994595136)2022-07-08 19:02:31,840:msa failed. aborting WARNING(140079994595136)2022-07-08 19:02:31,874:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-22_21_L001_R1_001.fastq.gz INFO(140079994595136)2022-07-08 19:02:31,874:finished processing per sample fasta files INFO(140079994595136)2022-07-08 19:02:31,874:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-22_21_L001_R1_001.fastq.gz finished INFO(140096826976064)2022-07-08 21:29:48,338:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-07_6_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140096826976064)2022-07-08 21:29:48,894:msa failed. aborting WARNING(140096826976064)2022-07-08 21:29:48,894:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-07_6_L001_R1_001.fastq.gz INFO(140096826976064)2022-07-08 21:29:48,894:finished processing per sample fasta files INFO(140096826976064)2022-07-08 21:29:48,894:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-07_6_L001_R1_001.fastq.gz finished INFO(140019243595584)2022-07-09 00:15:37,897:msa failed for file /tmp/tmp_yz2xtet/deblur_working_dir/MICRO-8855-17_16_L001_R1_001.fastq.gz.trim.derep.no_artifacts (maybe only 1 read?) WARNING(140019243595584)2022-07-09 00:15:38,594:msa failed. aborting WARNING(140019243595584)2022-07-09 00:15:38,594:deblurring failed for file /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-17_16_L001_R1_001.fastq.gz INFO(140019243595584)2022-07-09 00:15:38,594:finished processing per sample fasta files INFO(140019243595584)2022-07-09 00:15:38,594:worker thread for /tmp/qiime2-archive-irjixbbr/8fbef049-d032-4b6b-9175-29c7309e2b58/data/MICRO-8855-17_16_L001_R1_001.fastq.gz finished INFO(139987148605248)2022-07-09 00:15:44,739:create_otu_table for 0 samples, into output table /tmp/tmp_yz2xtet/all.biom INFO(139987148605248)2022-07-09 00:15:45,060:for output biom table loaded 0 samples, 0 unique sequences INFO(139987148605248)2022-07-09 00:15:45,295:keeping 0 (out of 0 sequences) with >=10 reads INFO(139987148605248)2022-07-09 00:15:47,279:saved to biom file /tmp/tmp_yz2xtet/all.biom INFO(139987148605248)2022-07-09 00:15:47,279:saved sequence fasta file to /tmp/tmp_yz2xtet/all.seqs.fa INFO(139987148605248)2022-07-09 00:15:47,279:getting 16s sequences from the biom table INFO(139987148605248)2022-07-09 00:15:47,279:remove_artifacts_seqs file /tmp/tmp_yz2xtet/all.seqs.fa WARNING(139987148605248)2022-07-09 00:15:47,279:file /tmp/tmp_yz2xtet/all.seqs.fa has size 0, continuing WARNING(139987148605248)2022-07-09 00:15:47,279:No clean sequences in /tmp/tmp_yz2xtet/all.seqs.fa INFO(139987148605248)2022-07-09 00:15:47,279:Keeping temp files INFO(139987148605248)2022-07-09 00:15:47,279:deblur workflow finished INFO(139987148605248)2022-07-09 00:15:47,279:output saved to /tmp/tmp_yz2xtet/all.biom INFO(139987148605248)2022-07-09 00:15:47,279:------------------ INFO(140267207894848)2022-07-12 10:11:52,043:************************* INFO(140267207894848)2022-07-12 10:11:52,088:deblurring started WARNING(140267207894848)2022-07-12 10:11:52,088:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data WARNING(140267207894848)2022-07-12 10:11:52,088:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': (), 'neg_ref_fp': (), 'neg_ref_db_fp': (), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 6, 'is_worker_thread': False, 'logger': } INFO(140267207894848)2022-07-12 10:11:52,088:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140267207894848)2022-07-12 10:11:52,088:deblur main program started INFO(140267207894848)2022-07-12 10:11:52,088:processing directory /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data INFO(140267207894848)2022-07-12 10:11:52,089:building negative db sortmerna index files INFO(140267207894848)2022-07-12 10:11:52,089:build_index_sortmerna files ['/home/ubuntu/Software/miniconda3/envs/qiime2-2022.2/lib/python3.8/site-packages/deblur/support_files/artifacts.fa'] to dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140267207894848)2022-07-12 10:11:52,291:building positive db sortmerna index files INFO(140267207894848)2022-07-12 10:11:52,291:build_index_sortmerna files ['/home/ubuntu/Software/miniconda3/envs/qiime2-2022.2/lib/python3.8/site-packages/deblur/support_files/88_otus.fasta'] to dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140267207894848)2022-07-12 10:12:23,896:parallel processing per sample fasta files INFO(140267207894848)2022-07-12 10:12:23,896:parallel deblur started for 36 inputs INFO(139650693134144)2022-07-12 10:12:24,624:************************* INFO(140121797142336)2022-07-12 10:12:24,624:************************* INFO(139650693134144)2022-07-12 10:12:24,624:deblurring started INFO(140121797142336)2022-07-12 10:12:24,624:deblurring started WARNING(140121797142336)2022-07-12 10:12:24,624:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-34_33_L001_R1_001.fastq.gz WARNING(139650693134144)2022-07-12 10:12:24,624:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-13_12_L001_R1_001.fastq.gz WARNING(140121797142336)2022-07-12 10:12:24,624:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-34_33_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } WARNING(139650693134144)2022-07-12 10:12:24,624:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-13_12_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140121797142336)2022-07-12 10:12:24,624:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139650693134144)2022-07-12 10:12:24,624:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140121797142336)2022-07-12 10:12:24,624:deblur worker thread started INFO(139650693134144)2022-07-12 10:12:24,624:deblur worker thread started INFO(140121797142336)2022-07-12 10:12:24,624:processing per sample fasta files INFO(139650693134144)2022-07-12 10:12:24,624:processing per sample fasta files INFO(140121797142336)2022-07-12 10:12:24,624:-------------------------------------------------------- INFO(139650693134144)2022-07-12 10:12:24,624:-------------------------------------------------------- INFO(140121797142336)2022-07-12 10:12:24,624:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-34_33_L001_R1_001.fastq.gz INFO(139650693134144)2022-07-12 10:12:24,624:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-13_12_L001_R1_001.fastq.gz INFO(140571933890368)2022-07-12 10:12:24,920:************************* INFO(140571933890368)2022-07-12 10:12:24,920:deblurring started WARNING(140571933890368)2022-07-12 10:12:24,920:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-31_30_L001_R1_001.fastq.gz WARNING(140571933890368)2022-07-12 10:12:24,920:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-31_30_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140571933890368)2022-07-12 10:12:24,920:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140571933890368)2022-07-12 10:12:24,920:deblur worker thread started INFO(140571933890368)2022-07-12 10:12:24,920:processing per sample fasta files INFO(140571933890368)2022-07-12 10:12:24,920:-------------------------------------------------------- INFO(140571933890368)2022-07-12 10:12:24,920:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-31_30_L001_R1_001.fastq.gz INFO(140162685421376)2022-07-12 10:12:24,930:************************* INFO(140162685421376)2022-07-12 10:12:24,930:deblurring started WARNING(140162685421376)2022-07-12 10:12:24,930:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-11_10_L001_R1_001.fastq.gz WARNING(140162685421376)2022-07-12 10:12:24,930:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-11_10_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140162685421376)2022-07-12 10:12:24,930:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140162685421376)2022-07-12 10:12:24,930:deblur worker thread started INFO(140162685421376)2022-07-12 10:12:24,930:processing per sample fasta files INFO(140162685421376)2022-07-12 10:12:24,930:-------------------------------------------------------- INFO(140162685421376)2022-07-12 10:12:24,930:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-11_10_L001_R1_001.fastq.gz INFO(140267757008704)2022-07-12 10:12:24,959:************************* INFO(140471184009024)2022-07-12 10:12:24,959:************************* INFO(140471184009024)2022-07-12 10:12:24,959:deblurring started INFO(140267757008704)2022-07-12 10:12:24,959:deblurring started WARNING(140267757008704)2022-07-12 10:12:24,959:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-30_29_L001_R1_001.fastq.gz WARNING(140471184009024)2022-07-12 10:12:24,959:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-04_3_L001_R1_001.fastq.gz WARNING(140267757008704)2022-07-12 10:12:24,959:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-30_29_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } WARNING(140471184009024)2022-07-12 10:12:24,959:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-04_3_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140267757008704)2022-07-12 10:12:24,959:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140471184009024)2022-07-12 10:12:24,959:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140267757008704)2022-07-12 10:12:24,959:deblur worker thread started INFO(140471184009024)2022-07-12 10:12:24,959:deblur worker thread started INFO(140471184009024)2022-07-12 10:12:24,959:processing per sample fasta files INFO(140267757008704)2022-07-12 10:12:24,959:processing per sample fasta files INFO(140471184009024)2022-07-12 10:12:24,959:-------------------------------------------------------- INFO(140267757008704)2022-07-12 10:12:24,959:-------------------------------------------------------- INFO(140471184009024)2022-07-12 10:12:24,959:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-04_3_L001_R1_001.fastq.gz INFO(140267757008704)2022-07-12 10:12:24,959:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-30_29_L001_R1_001.fastq.gz INFO(140121797142336)2022-07-12 10:24:05,564:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-34_33_L001_R1_001.fastq.gz.trim INFO(140121797142336)2022-07-12 10:24:05,891:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-34_33_L001_R1_001.fastq.gz.trim.derep INFO(140121797142336)2022-07-12 10:24:06,865:total sequences 18245, passing sequences 18245, failing sequences 0 INFO(140121797142336)2022-07-12 10:24:06,865:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-34_33_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140471184009024)2022-07-12 10:26:42,670:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-04_3_L001_R1_001.fastq.gz.trim INFO(140471184009024)2022-07-12 10:26:43,174:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-04_3_L001_R1_001.fastq.gz.trim.derep INFO(140471184009024)2022-07-12 10:26:44,503:total sequences 28314, passing sequences 28314, failing sequences 0 INFO(140471184009024)2022-07-12 10:26:44,503:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-04_3_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140267757008704)2022-07-12 10:32:56,514:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-30_29_L001_R1_001.fastq.gz.trim INFO(140267757008704)2022-07-12 10:32:57,039:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-30_29_L001_R1_001.fastq.gz.trim.derep INFO(140267757008704)2022-07-12 10:32:58,510:total sequences 30112, passing sequences 30112, failing sequences 0 INFO(140267757008704)2022-07-12 10:32:58,510:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-30_29_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140121797142336)2022-07-12 10:33:05,032:deblurring 18245 sequences INFO(140571933890368)2022-07-12 10:33:50,575:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim INFO(140571933890368)2022-07-12 10:33:51,242:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim.derep INFO(140571933890368)2022-07-12 10:33:52,905:total sequences 37350, passing sequences 37350, failing sequences 0 INFO(140571933890368)2022-07-12 10:33:52,905:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139650693134144)2022-07-12 10:34:11,713:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim INFO(139650693134144)2022-07-12 10:34:12,374:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim.derep INFO(139650693134144)2022-07-12 10:34:13,903:total sequences 33870, passing sequences 33870, failing sequences 0 INFO(139650693134144)2022-07-12 10:34:13,903:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140162685421376)2022-07-12 10:35:48,140:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim INFO(140162685421376)2022-07-12 10:35:48,907:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim.derep INFO(140162685421376)2022-07-12 10:35:50,511:total sequences 37223, passing sequences 37223, failing sequences 0 INFO(140162685421376)2022-07-12 10:35:50,511:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140121797142336)2022-07-12 10:45:14,316:18245 unique sequences left following deblurring INFO(140121797142336)2022-07-12 10:45:15,032:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-34_33_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140121797142336)2022-07-12 10:45:16,906:finished processing file INFO(140121797142336)2022-07-12 10:45:16,932:finished processing per sample fasta files INFO(140121797142336)2022-07-12 10:45:16,932:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-34_33_L001_R1_001.fastq.gz finished INFO(139882144737088)2022-07-12 10:45:20,291:************************* INFO(139882144737088)2022-07-12 10:45:20,291:deblurring started WARNING(139882144737088)2022-07-12 10:45:20,291:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-24_23_L001_R1_001.fastq.gz WARNING(139882144737088)2022-07-12 10:45:20,291:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-24_23_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139882144737088)2022-07-12 10:45:20,291:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139882144737088)2022-07-12 10:45:20,291:deblur worker thread started INFO(139882144737088)2022-07-12 10:45:20,291:processing per sample fasta files INFO(139882144737088)2022-07-12 10:45:20,291:-------------------------------------------------------- INFO(139882144737088)2022-07-12 10:45:20,291:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-24_23_L001_R1_001.fastq.gz INFO(140471184009024)2022-07-12 10:51:10,844:deblurring 28314 sequences INFO(140267757008704)2022-07-12 10:58:28,252:deblurring 30112 sequences INFO(139882144737088)2022-07-12 11:05:07,069:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-24_23_L001_R1_001.fastq.gz.trim INFO(139882144737088)2022-07-12 11:05:07,559:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-24_23_L001_R1_001.fastq.gz.trim.derep INFO(139882144737088)2022-07-12 11:05:08,905:total sequences 27677, passing sequences 27677, failing sequences 0 INFO(139882144737088)2022-07-12 11:05:08,905:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-24_23_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139650693134144)2022-07-12 11:09:29,329:deblurring 33870 sequences INFO(140571933890368)2022-07-12 11:16:19,081:deblurring 37350 sequences INFO(140162685421376)2022-07-12 11:16:33,976:deblurring 37223 sequences INFO(140471184009024)2022-07-12 11:22:09,272:28314 unique sequences left following deblurring INFO(140471184009024)2022-07-12 11:22:10,664:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-04_3_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140471184009024)2022-07-12 11:22:14,063:finished processing file INFO(140471184009024)2022-07-12 11:22:14,107:finished processing per sample fasta files INFO(140471184009024)2022-07-12 11:22:14,107:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-04_3_L001_R1_001.fastq.gz finished INFO(140290155607872)2022-07-12 11:22:15,071:************************* INFO(140290155607872)2022-07-12 11:22:15,071:deblurring started WARNING(140290155607872)2022-07-12 11:22:15,071:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-35_34_L001_R1_001.fastq.gz WARNING(140290155607872)2022-07-12 11:22:15,071:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-35_34_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140290155607872)2022-07-12 11:22:15,071:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140290155607872)2022-07-12 11:22:15,071:deblur worker thread started INFO(140290155607872)2022-07-12 11:22:15,071:processing per sample fasta files INFO(140290155607872)2022-07-12 11:22:15,071:-------------------------------------------------------- INFO(140290155607872)2022-07-12 11:22:15,071:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-35_34_L001_R1_001.fastq.gz INFO(139882144737088)2022-07-12 11:28:36,420:deblurring 27677 sequences INFO(140267757008704)2022-07-12 11:35:23,305:30112 unique sequences left following deblurring INFO(140267757008704)2022-07-12 11:35:24,843:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-30_29_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140267757008704)2022-07-12 11:35:28,927:finished processing file INFO(140267757008704)2022-07-12 11:35:28,974:finished processing per sample fasta files INFO(140267757008704)2022-07-12 11:35:28,974:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-30_29_L001_R1_001.fastq.gz finished INFO(140054270203712)2022-07-12 11:35:29,866:************************* INFO(140054270203712)2022-07-12 11:35:29,866:deblurring started WARNING(140054270203712)2022-07-12 11:35:29,866:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-28_27_L001_R1_001.fastq.gz WARNING(140054270203712)2022-07-12 11:35:29,866:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-28_27_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140054270203712)2022-07-12 11:35:29,866:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140054270203712)2022-07-12 11:35:29,866:deblur worker thread started INFO(140054270203712)2022-07-12 11:35:29,866:processing per sample fasta files INFO(140054270203712)2022-07-12 11:35:29,866:-------------------------------------------------------- INFO(140054270203712)2022-07-12 11:35:29,866:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-28_27_L001_R1_001.fastq.gz INFO(140054270203712)2022-07-12 11:46:03,916:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-28_27_L001_R1_001.fastq.gz.trim INFO(140054270203712)2022-07-12 11:46:04,223:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-28_27_L001_R1_001.fastq.gz.trim.derep INFO(140054270203712)2022-07-12 11:46:05,549:total sequences 22191, passing sequences 22191, failing sequences 0 INFO(140054270203712)2022-07-12 11:46:05,549:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-28_27_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140290155607872)2022-07-12 11:49:06,399:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-35_34_L001_R1_001.fastq.gz.trim INFO(140290155607872)2022-07-12 11:49:07,322:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-35_34_L001_R1_001.fastq.gz.trim.derep INFO(140290155607872)2022-07-12 11:49:09,351:total sequences 39890, passing sequences 39890, failing sequences 0 INFO(140290155607872)2022-07-12 11:49:09,352:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-35_34_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139650693134144)2022-07-12 11:58:08,034:33870 unique sequences left following deblurring INFO(139650693134144)2022-07-12 11:58:10,032:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-13_12_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139650693134144)2022-07-12 11:58:14,529:finished processing file INFO(139650693134144)2022-07-12 11:58:14,570:finished processing per sample fasta files INFO(139650693134144)2022-07-12 11:58:14,570:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-13_12_L001_R1_001.fastq.gz finished INFO(140694010607424)2022-07-12 11:58:15,694:************************* INFO(140694010607424)2022-07-12 11:58:15,694:deblurring started WARNING(140694010607424)2022-07-12 11:58:15,694:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-09_8_L001_R1_001.fastq.gz WARNING(140694010607424)2022-07-12 11:58:15,694:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-09_8_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140694010607424)2022-07-12 11:58:15,694:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140694010607424)2022-07-12 11:58:15,694:deblur worker thread started INFO(140694010607424)2022-07-12 11:58:15,694:processing per sample fasta files INFO(140694010607424)2022-07-12 11:58:15,694:-------------------------------------------------------- INFO(140694010607424)2022-07-12 11:58:15,694:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-09_8_L001_R1_001.fastq.gz INFO(139882144737088)2022-07-12 12:00:40,859:27677 unique sequences left following deblurring INFO(139882144737088)2022-07-12 12:00:42,299:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-24_23_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139882144737088)2022-07-12 12:00:45,645:finished processing file INFO(139882144737088)2022-07-12 12:00:45,687:finished processing per sample fasta files INFO(139882144737088)2022-07-12 12:00:45,687:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-24_23_L001_R1_001.fastq.gz finished INFO(140595890231104)2022-07-12 12:00:46,625:************************* INFO(140595890231104)2022-07-12 12:00:46,625:deblurring started WARNING(140595890231104)2022-07-12 12:00:46,625:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-10_9_L001_R1_001.fastq.gz WARNING(140595890231104)2022-07-12 12:00:46,625:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-10_9_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140595890231104)2022-07-12 12:00:46,625:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140595890231104)2022-07-12 12:00:46,625:deblur worker thread started INFO(140595890231104)2022-07-12 12:00:46,625:processing per sample fasta files INFO(140595890231104)2022-07-12 12:00:46,625:-------------------------------------------------------- INFO(140595890231104)2022-07-12 12:00:46,625:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-10_9_L001_R1_001.fastq.gz INFO(140054270203712)2022-07-12 12:00:50,603:deblurring 22191 sequences INFO(140571933890368)2022-07-12 12:14:16,570:37349 unique sequences left following deblurring INFO(140571933890368)2022-07-12 12:14:18,808:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-31_30_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140571933890368)2022-07-12 12:14:23,691:finished processing file INFO(140571933890368)2022-07-12 12:14:23,750:finished processing per sample fasta files INFO(140571933890368)2022-07-12 12:14:23,750:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-31_30_L001_R1_001.fastq.gz finished INFO(140449118070592)2022-07-12 12:14:24,633:************************* INFO(140449118070592)2022-07-12 12:14:24,633:deblurring started WARNING(140449118070592)2022-07-12 12:14:24,633:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-01_0_L001_R1_001.fastq.gz WARNING(140449118070592)2022-07-12 12:14:24,633:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-01_0_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140449118070592)2022-07-12 12:14:24,633:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140449118070592)2022-07-12 12:14:24,633:deblur worker thread started INFO(140449118070592)2022-07-12 12:14:24,633:processing per sample fasta files INFO(140449118070592)2022-07-12 12:14:24,633:-------------------------------------------------------- INFO(140449118070592)2022-07-12 12:14:24,633:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-01_0_L001_R1_001.fastq.gz INFO(140694010607424)2022-07-12 12:16:40,699:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim INFO(140694010607424)2022-07-12 12:16:41,198:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim.derep INFO(140694010607424)2022-07-12 12:16:42,672:total sequences 30401, passing sequences 30401, failing sequences 0 INFO(140694010607424)2022-07-12 12:16:42,672:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140162685421376)2022-07-12 12:17:10,755:37223 unique sequences left following deblurring INFO(140162685421376)2022-07-12 12:17:13,108:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-11_10_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140162685421376)2022-07-12 12:17:17,881:finished processing file INFO(140162685421376)2022-07-12 12:17:17,933:finished processing per sample fasta files INFO(140162685421376)2022-07-12 12:17:17,933:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-11_10_L001_R1_001.fastq.gz finished INFO(139955768276800)2022-07-12 12:17:18,961:************************* INFO(139955768276800)2022-07-12 12:17:18,962:deblurring started WARNING(139955768276800)2022-07-12 12:17:18,962:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-33_32_L001_R1_001.fastq.gz WARNING(139955768276800)2022-07-12 12:17:18,962:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-33_32_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139955768276800)2022-07-12 12:17:18,962:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139955768276800)2022-07-12 12:17:18,962:deblur worker thread started INFO(139955768276800)2022-07-12 12:17:18,962:processing per sample fasta files INFO(139955768276800)2022-07-12 12:17:18,962:-------------------------------------------------------- INFO(139955768276800)2022-07-12 12:17:18,962:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-33_32_L001_R1_001.fastq.gz INFO(140054270203712)2022-07-12 12:19:21,995:22191 unique sequences left following deblurring INFO(140054270203712)2022-07-12 12:19:23,024:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-28_27_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140054270203712)2022-07-12 12:19:25,422:finished processing file INFO(140054270203712)2022-07-12 12:19:25,444:finished processing per sample fasta files INFO(140054270203712)2022-07-12 12:19:25,444:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-28_27_L001_R1_001.fastq.gz finished INFO(139744972941120)2022-07-12 12:19:26,238:************************* INFO(139744972941120)2022-07-12 12:19:26,238:deblurring started WARNING(139744972941120)2022-07-12 12:19:26,238:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-23_22_L001_R1_001.fastq.gz WARNING(139744972941120)2022-07-12 12:19:26,239:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-23_22_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139744972941120)2022-07-12 12:19:26,239:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139744972941120)2022-07-12 12:19:26,239:deblur worker thread started INFO(139744972941120)2022-07-12 12:19:26,239:processing per sample fasta files INFO(139744972941120)2022-07-12 12:19:26,239:-------------------------------------------------------- INFO(139744972941120)2022-07-12 12:19:26,239:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-23_22_L001_R1_001.fastq.gz INFO(140595890231104)2022-07-12 12:20:15,858:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-10_9_L001_R1_001.fastq.gz.trim INFO(140595890231104)2022-07-12 12:20:16,359:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-10_9_L001_R1_001.fastq.gz.trim.derep INFO(140595890231104)2022-07-12 12:20:17,841:total sequences 31114, passing sequences 31114, failing sequences 0 INFO(140595890231104)2022-07-12 12:20:17,841:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-10_9_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140449118070592)2022-07-12 12:32:15,557:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim INFO(140449118070592)2022-07-12 12:32:16,161:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim.derep INFO(140449118070592)2022-07-12 12:32:17,804:total sequences 34148, passing sequences 34148, failing sequences 0 INFO(140449118070592)2022-07-12 12:32:17,804:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139744972941120)2022-07-12 12:35:21,985:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-23_22_L001_R1_001.fastq.gz.trim INFO(139744972941120)2022-07-12 12:35:22,482:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-23_22_L001_R1_001.fastq.gz.trim.derep INFO(139744972941120)2022-07-12 12:35:23,627:total sequences 21731, passing sequences 21731, failing sequences 0 INFO(139744972941120)2022-07-12 12:35:23,627:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-23_22_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140290155607872)2022-07-12 12:36:19,239:deblurring 39890 sequences INFO(140694010607424)2022-07-12 12:43:19,544:deblurring 30401 sequences INFO(139955768276800)2022-07-12 12:43:53,415:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim INFO(139955768276800)2022-07-12 12:43:54,518:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim.derep INFO(139955768276800)2022-07-12 12:43:56,219:total sequences 36340, passing sequences 36340, failing sequences 0 INFO(139955768276800)2022-07-12 12:43:56,219:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139744972941120)2022-07-12 12:49:41,141:deblurring 21731 sequences INFO(140595890231104)2022-07-12 12:49:56,432:deblurring 31114 sequences INFO(140449118070592)2022-07-12 13:07:19,223:deblurring 34148 sequences INFO(139744972941120)2022-07-12 13:07:38,285:21731 unique sequences left following deblurring INFO(139744972941120)2022-07-12 13:07:39,301:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-23_22_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139744972941120)2022-07-12 13:07:41,671:finished processing file INFO(139744972941120)2022-07-12 13:07:41,705:finished processing per sample fasta files INFO(139744972941120)2022-07-12 13:07:41,705:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-23_22_L001_R1_001.fastq.gz finished INFO(140127792658240)2022-07-12 13:07:42,590:************************* INFO(140127792658240)2022-07-12 13:07:42,590:deblurring started WARNING(140127792658240)2022-07-12 13:07:42,590:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-21_20_L001_R1_001.fastq.gz WARNING(140127792658240)2022-07-12 13:07:42,590:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-21_20_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140127792658240)2022-07-12 13:07:42,590:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140127792658240)2022-07-12 13:07:42,590:deblur worker thread started INFO(140127792658240)2022-07-12 13:07:42,590:processing per sample fasta files INFO(140127792658240)2022-07-12 13:07:42,590:-------------------------------------------------------- INFO(140127792658240)2022-07-12 13:07:42,590:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-21_20_L001_R1_001.fastq.gz INFO(140694010607424)2022-07-12 13:20:57,043:30401 unique sequences left following deblurring INFO(140694010607424)2022-07-12 13:20:58,709:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-09_8_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140694010607424)2022-07-12 13:21:02,557:finished processing file INFO(140694010607424)2022-07-12 13:21:02,589:finished processing per sample fasta files INFO(140694010607424)2022-07-12 13:21:02,590:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-09_8_L001_R1_001.fastq.gz finished INFO(140069042624320)2022-07-12 13:21:03,429:************************* INFO(140069042624320)2022-07-12 13:21:03,429:deblurring started WARNING(140069042624320)2022-07-12 13:21:03,429:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-05_4_L001_R1_001.fastq.gz WARNING(140069042624320)2022-07-12 13:21:03,429:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-05_4_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140069042624320)2022-07-12 13:21:03,429:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140069042624320)2022-07-12 13:21:03,429:deblur worker thread started INFO(140069042624320)2022-07-12 13:21:03,429:processing per sample fasta files INFO(140069042624320)2022-07-12 13:21:03,429:-------------------------------------------------------- INFO(140069042624320)2022-07-12 13:21:03,429:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-05_4_L001_R1_001.fastq.gz INFO(139955768276800)2022-07-12 13:22:29,752:deblurring 36340 sequences INFO(140127792658240)2022-07-12 13:27:40,002:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-21_20_L001_R1_001.fastq.gz.trim INFO(140127792658240)2022-07-12 13:27:40,643:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-21_20_L001_R1_001.fastq.gz.trim.derep INFO(140127792658240)2022-07-12 13:27:42,272:total sequences 34155, passing sequences 34155, failing sequences 0 INFO(140127792658240)2022-07-12 13:27:42,272:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-21_20_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140595890231104)2022-07-12 13:29:22,699:31114 unique sequences left following deblurring INFO(140595890231104)2022-07-12 13:29:24,263:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-10_9_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140595890231104)2022-07-12 13:29:28,074:finished processing file INFO(140595890231104)2022-07-12 13:29:28,106:finished processing per sample fasta files INFO(140595890231104)2022-07-12 13:29:28,106:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-10_9_L001_R1_001.fastq.gz finished INFO(140433650501440)2022-07-12 13:29:28,948:************************* INFO(140433650501440)2022-07-12 13:29:28,948:deblurring started WARNING(140433650501440)2022-07-12 13:29:28,949:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-29_28_L001_R1_001.fastq.gz WARNING(140433650501440)2022-07-12 13:29:28,949:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-29_28_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140433650501440)2022-07-12 13:29:28,949:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140433650501440)2022-07-12 13:29:28,949:deblur worker thread started INFO(140433650501440)2022-07-12 13:29:28,949:processing per sample fasta files INFO(140433650501440)2022-07-12 13:29:28,949:-------------------------------------------------------- INFO(140433650501440)2022-07-12 13:29:28,949:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-29_28_L001_R1_001.fastq.gz INFO(140290155607872)2022-07-12 13:44:00,278:39890 unique sequences left following deblurring INFO(140290155607872)2022-07-12 13:44:02,685:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-35_34_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140290155607872)2022-07-12 13:44:07,919:finished processing file INFO(140290155607872)2022-07-12 13:44:07,964:finished processing per sample fasta files INFO(140290155607872)2022-07-12 13:44:07,964:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-35_34_L001_R1_001.fastq.gz finished INFO(140266511882048)2022-07-12 13:44:08,811:************************* INFO(140266511882048)2022-07-12 13:44:08,811:deblurring started WARNING(140266511882048)2022-07-12 13:44:08,811:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-02_1_L001_R1_001.fastq.gz WARNING(140266511882048)2022-07-12 13:44:08,811:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-02_1_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140266511882048)2022-07-12 13:44:08,811:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140266511882048)2022-07-12 13:44:08,811:deblur worker thread started INFO(140266511882048)2022-07-12 13:44:08,811:processing per sample fasta files INFO(140266511882048)2022-07-12 13:44:08,811:-------------------------------------------------------- INFO(140266511882048)2022-07-12 13:44:08,811:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-02_1_L001_R1_001.fastq.gz INFO(140069042624320)2022-07-12 13:45:13,429:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-05_4_L001_R1_001.fastq.gz.trim INFO(140069042624320)2022-07-12 13:45:14,155:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-05_4_L001_R1_001.fastq.gz.trim.derep INFO(140069042624320)2022-07-12 13:45:16,042:total sequences 41239, passing sequences 41239, failing sequences 0 INFO(140069042624320)2022-07-12 13:45:16,043:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-05_4_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140433650501440)2022-07-12 13:46:38,287:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-29_28_L001_R1_001.fastq.gz.trim INFO(140433650501440)2022-07-12 13:46:38,752:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-29_28_L001_R1_001.fastq.gz.trim.derep INFO(140433650501440)2022-07-12 13:46:40,136:total sequences 28510, passing sequences 28510, failing sequences 0 INFO(140433650501440)2022-07-12 13:46:40,136:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-29_28_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140449118070592)2022-07-12 13:51:39,868:34148 unique sequences left following deblurring INFO(140449118070592)2022-07-12 13:51:41,587:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-01_0_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140449118070592)2022-07-12 13:51:46,045:finished processing file INFO(140449118070592)2022-07-12 13:51:46,082:finished processing per sample fasta files INFO(140449118070592)2022-07-12 13:51:46,082:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-01_0_L001_R1_001.fastq.gz finished INFO(139909380708160)2022-07-12 13:51:46,898:************************* INFO(139909380708160)2022-07-12 13:51:46,898:deblurring started WARNING(139909380708160)2022-07-12 13:51:46,898:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-25_24_L001_R1_001.fastq.gz WARNING(139909380708160)2022-07-12 13:51:46,898:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-25_24_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139909380708160)2022-07-12 13:51:46,898:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139909380708160)2022-07-12 13:51:46,898:deblur worker thread started INFO(139909380708160)2022-07-12 13:51:46,898:processing per sample fasta files INFO(139909380708160)2022-07-12 13:51:46,898:-------------------------------------------------------- INFO(139909380708160)2022-07-12 13:51:46,898:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-25_24_L001_R1_001.fastq.gz INFO(140127792658240)2022-07-12 14:03:02,625:deblurring 34155 sequences INFO(140266511882048)2022-07-12 14:05:58,673:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-02_1_L001_R1_001.fastq.gz.trim INFO(140266511882048)2022-07-12 14:05:59,337:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-02_1_L001_R1_001.fastq.gz.trim.derep INFO(140266511882048)2022-07-12 14:06:00,977:total sequences 33811, passing sequences 33811, failing sequences 0 INFO(140266511882048)2022-07-12 14:06:00,977:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-02_1_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140433650501440)2022-07-12 14:09:42,377:deblurring 28510 sequences INFO(139909380708160)2022-07-12 14:13:06,384:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-25_24_L001_R1_001.fastq.gz.trim INFO(139909380708160)2022-07-12 14:13:06,961:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-25_24_L001_R1_001.fastq.gz.trim.derep INFO(139909380708160)2022-07-12 14:13:08,681:total sequences 32735, passing sequences 32735, failing sequences 0 INFO(139909380708160)2022-07-12 14:13:08,681:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-25_24_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139955768276800)2022-07-12 14:14:37,974:36340 unique sequences left following deblurring INFO(139955768276800)2022-07-12 14:14:39,994:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-33_32_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139955768276800)2022-07-12 14:14:45,114:finished processing file INFO(139955768276800)2022-07-12 14:14:45,175:finished processing per sample fasta files INFO(139955768276800)2022-07-12 14:14:45,175:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-33_32_L001_R1_001.fastq.gz finished INFO(139991119292224)2022-07-12 14:14:46,168:************************* INFO(139991119292224)2022-07-12 14:14:46,168:deblurring started WARNING(139991119292224)2022-07-12 14:14:46,168:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-18_17_L001_R1_001.fastq.gz WARNING(139991119292224)2022-07-12 14:14:46,168:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-18_17_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139991119292224)2022-07-12 14:14:46,168:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139991119292224)2022-07-12 14:14:46,168:deblur worker thread started INFO(139991119292224)2022-07-12 14:14:46,168:processing per sample fasta files INFO(139991119292224)2022-07-12 14:14:46,168:-------------------------------------------------------- INFO(139991119292224)2022-07-12 14:14:46,168:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-18_17_L001_R1_001.fastq.gz INFO(140069042624320)2022-07-12 14:34:27,327:deblurring 41239 sequences INFO(139991119292224)2022-07-12 14:37:21,037:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-18_17_L001_R1_001.fastq.gz.trim INFO(139991119292224)2022-07-12 14:37:21,747:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-18_17_L001_R1_001.fastq.gz.trim.derep INFO(139991119292224)2022-07-12 14:37:23,633:total sequences 39209, passing sequences 39209, failing sequences 0 INFO(139991119292224)2022-07-12 14:37:23,634:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-18_17_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140266511882048)2022-07-12 14:40:38,858:deblurring 33811 sequences INFO(140433650501440)2022-07-12 14:42:44,268:28510 unique sequences left following deblurring INFO(140433650501440)2022-07-12 14:42:45,860:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-29_28_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140433650501440)2022-07-12 14:42:49,420:finished processing file INFO(140433650501440)2022-07-12 14:42:49,461:finished processing per sample fasta files INFO(140433650501440)2022-07-12 14:42:49,461:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-29_28_L001_R1_001.fastq.gz finished INFO(140424794478400)2022-07-12 14:42:50,331:************************* INFO(140424794478400)2022-07-12 14:42:50,331:deblurring started WARNING(140424794478400)2022-07-12 14:42:50,331:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-12_11_L001_R1_001.fastq.gz WARNING(140424794478400)2022-07-12 14:42:50,331:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-12_11_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140424794478400)2022-07-12 14:42:50,332:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140424794478400)2022-07-12 14:42:50,332:deblur worker thread started INFO(140424794478400)2022-07-12 14:42:50,332:processing per sample fasta files INFO(140424794478400)2022-07-12 14:42:50,332:-------------------------------------------------------- INFO(140424794478400)2022-07-12 14:42:50,332:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-12_11_L001_R1_001.fastq.gz INFO(139909380708160)2022-07-12 14:45:02,498:deblurring 32735 sequences INFO(140127792658240)2022-07-12 14:55:11,700:34155 unique sequences left following deblurring INFO(140127792658240)2022-07-12 14:55:13,866:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-21_20_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140127792658240)2022-07-12 14:55:18,313:finished processing file INFO(140127792658240)2022-07-12 14:55:18,357:finished processing per sample fasta files INFO(140127792658240)2022-07-12 14:55:18,357:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-21_20_L001_R1_001.fastq.gz finished INFO(140335888906048)2022-07-12 14:55:19,450:************************* INFO(140335888906048)2022-07-12 14:55:19,450:deblurring started WARNING(140335888906048)2022-07-12 14:55:19,451:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-14_13_L001_R1_001.fastq.gz WARNING(140335888906048)2022-07-12 14:55:19,451:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-14_13_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140335888906048)2022-07-12 14:55:19,451:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140335888906048)2022-07-12 14:55:19,451:deblur worker thread started INFO(140335888906048)2022-07-12 14:55:19,451:processing per sample fasta files INFO(140335888906048)2022-07-12 14:55:19,451:-------------------------------------------------------- INFO(140335888906048)2022-07-12 14:55:19,451:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-14_13_L001_R1_001.fastq.gz INFO(140424794478400)2022-07-12 15:02:02,767:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-12_11_L001_R1_001.fastq.gz.trim INFO(140424794478400)2022-07-12 15:02:03,385:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-12_11_L001_R1_001.fastq.gz.trim.derep INFO(140424794478400)2022-07-12 15:02:04,986:total sequences 32986, passing sequences 32986, failing sequences 0 INFO(140424794478400)2022-07-12 15:02:04,986:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-12_11_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140335888906048)2022-07-12 15:16:55,488:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-14_13_L001_R1_001.fastq.gz.trim INFO(140335888906048)2022-07-12 15:16:56,135:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-14_13_L001_R1_001.fastq.gz.trim.derep INFO(140335888906048)2022-07-12 15:16:57,696:total sequences 31799, passing sequences 31799, failing sequences 0 INFO(140335888906048)2022-07-12 15:16:57,696:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-14_13_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140266511882048)2022-07-12 15:25:51,613:33811 unique sequences left following deblurring INFO(140266511882048)2022-07-12 15:25:53,425:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-02_1_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140266511882048)2022-07-12 15:25:58,063:finished processing file INFO(140266511882048)2022-07-12 15:25:58,100:finished processing per sample fasta files INFO(140266511882048)2022-07-12 15:25:58,100:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-02_1_L001_R1_001.fastq.gz finished INFO(140504788260672)2022-07-12 15:25:58,989:************************* INFO(140504788260672)2022-07-12 15:25:58,989:deblurring started WARNING(140504788260672)2022-07-12 15:25:58,989:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-08_7_L001_R1_001.fastq.gz WARNING(140504788260672)2022-07-12 15:25:58,989:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-08_7_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140504788260672)2022-07-12 15:25:58,989:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140504788260672)2022-07-12 15:25:58,989:deblur worker thread started INFO(140504788260672)2022-07-12 15:25:58,989:processing per sample fasta files INFO(140504788260672)2022-07-12 15:25:58,989:-------------------------------------------------------- INFO(140504788260672)2022-07-12 15:25:58,989:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-08_7_L001_R1_001.fastq.gz INFO(139909380708160)2022-07-12 15:27:49,598:32735 unique sequences left following deblurring INFO(139909380708160)2022-07-12 15:27:51,350:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-25_24_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139909380708160)2022-07-12 15:27:55,265:finished processing file INFO(139909380708160)2022-07-12 15:27:55,299:finished processing per sample fasta files INFO(139909380708160)2022-07-12 15:27:55,299:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-25_24_L001_R1_001.fastq.gz finished INFO(139622414092096)2022-07-12 15:27:56,105:************************* INFO(139622414092096)2022-07-12 15:27:56,105:deblurring started WARNING(139622414092096)2022-07-12 15:27:56,106:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-27_26_L001_R1_001.fastq.gz WARNING(139622414092096)2022-07-12 15:27:56,106:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-27_26_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139622414092096)2022-07-12 15:27:56,106:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139622414092096)2022-07-12 15:27:56,106:deblur worker thread started INFO(139622414092096)2022-07-12 15:27:56,106:processing per sample fasta files INFO(139622414092096)2022-07-12 15:27:56,106:-------------------------------------------------------- INFO(139622414092096)2022-07-12 15:27:56,106:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-27_26_L001_R1_001.fastq.gz INFO(139991119292224)2022-07-12 15:28:12,784:deblurring 39209 sequences INFO(140424794478400)2022-07-12 15:32:47,540:deblurring 32986 sequences INFO(139622414092096)2022-07-12 15:44:24,827:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-27_26_L001_R1_001.fastq.gz.trim INFO(139622414092096)2022-07-12 15:44:25,355:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-27_26_L001_R1_001.fastq.gz.trim.derep INFO(139622414092096)2022-07-12 15:44:26,779:total sequences 27516, passing sequences 27516, failing sequences 0 INFO(139622414092096)2022-07-12 15:44:26,779:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-27_26_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140335888906048)2022-07-12 15:45:22,286:deblurring 31799 sequences INFO(140069042624320)2022-07-12 15:49:42,447:41239 unique sequences left following deblurring INFO(140069042624320)2022-07-12 15:49:44,824:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-05_4_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140069042624320)2022-07-12 15:49:50,646:finished processing file INFO(140069042624320)2022-07-12 15:49:50,710:finished processing per sample fasta files INFO(140069042624320)2022-07-12 15:49:50,710:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-05_4_L001_R1_001.fastq.gz finished INFO(140383060633408)2022-07-12 15:49:51,621:************************* INFO(140383060633408)2022-07-12 15:49:51,621:deblurring started WARNING(140383060633408)2022-07-12 15:49:51,621:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-36_35_L001_R1_001.fastq.gz WARNING(140383060633408)2022-07-12 15:49:51,621:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-36_35_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140383060633408)2022-07-12 15:49:51,621:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140383060633408)2022-07-12 15:49:51,621:deblur worker thread started INFO(140383060633408)2022-07-12 15:49:51,621:processing per sample fasta files INFO(140383060633408)2022-07-12 15:49:51,621:-------------------------------------------------------- INFO(140383060633408)2022-07-12 15:49:51,621:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-36_35_L001_R1_001.fastq.gz INFO(140504788260672)2022-07-12 15:52:18,566:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-08_7_L001_R1_001.fastq.gz.trim INFO(140504788260672)2022-07-12 15:52:19,344:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-08_7_L001_R1_001.fastq.gz.trim.derep INFO(140504788260672)2022-07-12 15:52:21,413:total sequences 44779, passing sequences 44779, failing sequences 0 INFO(140504788260672)2022-07-12 15:52:21,413:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-08_7_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140383060633408)2022-07-12 16:04:32,878:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-36_35_L001_R1_001.fastq.gz.trim INFO(140383060633408)2022-07-12 16:04:33,322:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-36_35_L001_R1_001.fastq.gz.trim.derep INFO(140383060633408)2022-07-12 16:04:34,394:total sequences 18450, passing sequences 18450, failing sequences 0 INFO(140383060633408)2022-07-12 16:04:34,394:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-36_35_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139622414092096)2022-07-12 16:05:35,605:deblurring 27516 sequences INFO(140383060633408)2022-07-12 16:13:58,633:deblurring 18450 sequences INFO(140424794478400)2022-07-12 16:18:12,046:32986 unique sequences left following deblurring INFO(140424794478400)2022-07-12 16:18:13,943:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-12_11_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140424794478400)2022-07-12 16:18:18,118:finished processing file INFO(140424794478400)2022-07-12 16:18:18,155:finished processing per sample fasta files INFO(140424794478400)2022-07-12 16:18:18,155:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-12_11_L001_R1_001.fastq.gz finished INFO(139827588650816)2022-07-12 16:18:19,552:************************* INFO(139827588650816)2022-07-12 16:18:19,553:deblurring started WARNING(139827588650816)2022-07-12 16:18:19,553:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-15_14_L001_R1_001.fastq.gz WARNING(139827588650816)2022-07-12 16:18:19,553:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-15_14_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139827588650816)2022-07-12 16:18:19,553:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139827588650816)2022-07-12 16:18:19,553:deblur worker thread started INFO(139827588650816)2022-07-12 16:18:19,553:processing per sample fasta files INFO(139827588650816)2022-07-12 16:18:19,553:-------------------------------------------------------- INFO(139827588650816)2022-07-12 16:18:19,553:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-15_14_L001_R1_001.fastq.gz INFO(140383060633408)2022-07-12 16:26:06,228:18450 unique sequences left following deblurring INFO(140383060633408)2022-07-12 16:26:06,952:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-36_35_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140383060633408)2022-07-12 16:26:08,892:finished processing file INFO(140383060633408)2022-07-12 16:26:08,913:finished processing per sample fasta files INFO(140383060633408)2022-07-12 16:26:08,913:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-36_35_L001_R1_001.fastq.gz finished INFO(139635526711104)2022-07-12 16:26:09,768:************************* INFO(139635526711104)2022-07-12 16:26:09,768:deblurring started WARNING(139635526711104)2022-07-12 16:26:09,768:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-16_15_L001_R1_001.fastq.gz WARNING(139635526711104)2022-07-12 16:26:09,768:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-16_15_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139635526711104)2022-07-12 16:26:09,768:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139635526711104)2022-07-12 16:26:09,768:deblur worker thread started INFO(139635526711104)2022-07-12 16:26:09,768:processing per sample fasta files INFO(139635526711104)2022-07-12 16:26:09,769:-------------------------------------------------------- INFO(139635526711104)2022-07-12 16:26:09,769:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-16_15_L001_R1_001.fastq.gz INFO(140335888906048)2022-07-12 16:27:06,635:31799 unique sequences left following deblurring INFO(140335888906048)2022-07-12 16:27:08,514:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-14_13_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140335888906048)2022-07-12 16:27:12,409:finished processing file INFO(140335888906048)2022-07-12 16:27:12,445:finished processing per sample fasta files INFO(140335888906048)2022-07-12 16:27:12,445:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-14_13_L001_R1_001.fastq.gz finished INFO(140443028047680)2022-07-12 16:27:13,272:************************* INFO(140443028047680)2022-07-12 16:27:13,272:deblurring started WARNING(140443028047680)2022-07-12 16:27:13,272:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-26_25_L001_R1_001.fastq.gz WARNING(140443028047680)2022-07-12 16:27:13,272:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-26_25_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140443028047680)2022-07-12 16:27:13,272:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140443028047680)2022-07-12 16:27:13,272:deblur worker thread started INFO(140443028047680)2022-07-12 16:27:13,272:processing per sample fasta files INFO(140443028047680)2022-07-12 16:27:13,272:-------------------------------------------------------- INFO(140443028047680)2022-07-12 16:27:13,272:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-26_25_L001_R1_001.fastq.gz INFO(139622414092096)2022-07-12 16:34:14,150:27516 unique sequences left following deblurring INFO(139622414092096)2022-07-12 16:34:15,463:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-27_26_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139622414092096)2022-07-12 16:34:18,653:finished processing file INFO(139622414092096)2022-07-12 16:34:18,695:finished processing per sample fasta files INFO(139622414092096)2022-07-12 16:34:18,695:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-27_26_L001_R1_001.fastq.gz finished INFO(139656134977344)2022-07-12 16:34:19,555:************************* INFO(139656134977344)2022-07-12 16:34:19,556:deblurring started WARNING(139656134977344)2022-07-12 16:34:19,556:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-03_2_L001_R1_001.fastq.gz WARNING(139656134977344)2022-07-12 16:34:19,556:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-03_2_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139656134977344)2022-07-12 16:34:19,556:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139656134977344)2022-07-12 16:34:19,556:deblur worker thread started INFO(139656134977344)2022-07-12 16:34:19,556:processing per sample fasta files INFO(139656134977344)2022-07-12 16:34:19,556:-------------------------------------------------------- INFO(139656134977344)2022-07-12 16:34:19,556:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-03_2_L001_R1_001.fastq.gz INFO(139991119292224)2022-07-12 16:35:26,481:39209 unique sequences left following deblurring INFO(139991119292224)2022-07-12 16:35:29,042:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-18_17_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139991119292224)2022-07-12 16:35:34,131:finished processing file INFO(139991119292224)2022-07-12 16:35:34,183:finished processing per sample fasta files INFO(139991119292224)2022-07-12 16:35:34,183:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-18_17_L001_R1_001.fastq.gz finished INFO(139942697998144)2022-07-12 16:35:35,006:************************* INFO(139942697998144)2022-07-12 16:35:35,007:deblurring started WARNING(139942697998144)2022-07-12 16:35:35,007:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-20_19_L001_R1_001.fastq.gz WARNING(139942697998144)2022-07-12 16:35:35,007:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-20_19_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139942697998144)2022-07-12 16:35:35,007:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139942697998144)2022-07-12 16:35:35,007:deblur worker thread started INFO(139942697998144)2022-07-12 16:35:35,007:processing per sample fasta files INFO(139942697998144)2022-07-12 16:35:35,007:-------------------------------------------------------- INFO(139942697998144)2022-07-12 16:35:35,007:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-20_19_L001_R1_001.fastq.gz INFO(139827588650816)2022-07-12 16:36:01,208:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-15_14_L001_R1_001.fastq.gz.trim INFO(139827588650816)2022-07-12 16:36:01,653:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-15_14_L001_R1_001.fastq.gz.trim.derep INFO(139827588650816)2022-07-12 16:36:02,877:total sequences 25567, passing sequences 25567, failing sequences 0 INFO(139827588650816)2022-07-12 16:36:02,877:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-15_14_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139635526711104)2022-07-12 16:41:39,906:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-16_15_L001_R1_001.fastq.gz.trim INFO(139635526711104)2022-07-12 16:41:40,391:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-16_15_L001_R1_001.fastq.gz.trim.derep INFO(139635526711104)2022-07-12 16:41:42,091:total sequences 36976, passing sequences 36976, failing sequences 0 INFO(139635526711104)2022-07-12 16:41:42,092:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-16_15_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140504788260672)2022-07-12 16:48:19,640:deblurring 44779 sequences INFO(139656134977344)2022-07-12 16:48:38,958:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-03_2_L001_R1_001.fastq.gz.trim INFO(139656134977344)2022-07-12 16:48:39,391:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-03_2_L001_R1_001.fastq.gz.trim.derep INFO(139656134977344)2022-07-12 16:48:40,777:total sequences 27314, passing sequences 27314, failing sequences 0 INFO(139656134977344)2022-07-12 16:48:40,777:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-03_2_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139942697998144)2022-07-12 16:50:50,665:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-20_19_L001_R1_001.fastq.gz.trim INFO(139942697998144)2022-07-12 16:50:51,155:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-20_19_L001_R1_001.fastq.gz.trim.derep INFO(139942697998144)2022-07-12 16:50:52,464:total sequences 24882, passing sequences 24882, failing sequences 0 INFO(139942697998144)2022-07-12 16:50:52,464:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-20_19_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140443028047680)2022-07-12 16:51:27,641:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-26_25_L001_R1_001.fastq.gz.trim INFO(140443028047680)2022-07-12 16:51:28,328:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-26_25_L001_R1_001.fastq.gz.trim.derep INFO(140443028047680)2022-07-12 16:51:29,902:total sequences 32317, passing sequences 32317, failing sequences 0 INFO(140443028047680)2022-07-12 16:51:29,902:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-26_25_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139827588650816)2022-07-12 16:54:36,067:deblurring 25567 sequences INFO(139942697998144)2022-07-12 17:09:57,433:deblurring 24882 sequences INFO(139656134977344)2022-07-12 17:11:29,500:deblurring 27314 sequences INFO(140443028047680)2022-07-12 17:21:41,271:deblurring 32317 sequences INFO(139827588650816)2022-07-12 17:21:57,738:25567 unique sequences left following deblurring INFO(139827588650816)2022-07-12 17:21:59,548:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-15_14_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139827588650816)2022-07-12 17:22:02,717:finished processing file INFO(139827588650816)2022-07-12 17:22:02,748:finished processing per sample fasta files INFO(139827588650816)2022-07-12 17:22:02,748:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-15_14_L001_R1_001.fastq.gz finished INFO(140438718146368)2022-07-12 17:22:03,633:************************* INFO(140438718146368)2022-07-12 17:22:03,633:deblurring started WARNING(140438718146368)2022-07-12 17:22:03,633:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-06_5_L001_R1_001.fastq.gz WARNING(140438718146368)2022-07-12 17:22:03,633:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-06_5_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140438718146368)2022-07-12 17:22:03,633:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140438718146368)2022-07-12 17:22:03,634:deblur worker thread started INFO(140438718146368)2022-07-12 17:22:03,634:processing per sample fasta files INFO(140438718146368)2022-07-12 17:22:03,634:-------------------------------------------------------- INFO(140438718146368)2022-07-12 17:22:03,634:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-06_5_L001_R1_001.fastq.gz INFO(139635526711104)2022-07-12 17:24:51,054:deblurring 36976 sequences INFO(139942697998144)2022-07-12 17:34:37,199:24882 unique sequences left following deblurring INFO(139942697998144)2022-07-12 17:34:38,340:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-20_19_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139942697998144)2022-07-12 17:34:41,287:finished processing file INFO(139942697998144)2022-07-12 17:34:41,314:finished processing per sample fasta files INFO(139942697998144)2022-07-12 17:34:41,314:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-20_19_L001_R1_001.fastq.gz finished INFO(139989471684416)2022-07-12 17:34:42,157:************************* INFO(139989471684416)2022-07-12 17:34:42,157:deblurring started WARNING(139989471684416)2022-07-12 17:34:42,157:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-32_31_L001_R1_001.fastq.gz WARNING(139989471684416)2022-07-12 17:34:42,157:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-32_31_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139989471684416)2022-07-12 17:34:42,157:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139989471684416)2022-07-12 17:34:42,157:deblur worker thread started INFO(139989471684416)2022-07-12 17:34:42,157:processing per sample fasta files INFO(139989471684416)2022-07-12 17:34:42,157:-------------------------------------------------------- INFO(139989471684416)2022-07-12 17:34:42,157:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-32_31_L001_R1_001.fastq.gz INFO(140438718146368)2022-07-12 17:42:24,805:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-06_5_L001_R1_001.fastq.gz.trim INFO(140438718146368)2022-07-12 17:42:25,440:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-06_5_L001_R1_001.fastq.gz.trim.derep INFO(140438718146368)2022-07-12 17:42:26,930:total sequences 29704, passing sequences 29704, failing sequences 0 INFO(140438718146368)2022-07-12 17:42:26,930:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-06_5_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139656134977344)2022-07-12 17:44:25,148:27314 unique sequences left following deblurring INFO(139656134977344)2022-07-12 17:44:26,728:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-03_2_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139656134977344)2022-07-12 17:44:30,137:finished processing file INFO(139656134977344)2022-07-12 17:44:30,177:finished processing per sample fasta files INFO(139656134977344)2022-07-12 17:44:30,177:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-03_2_L001_R1_001.fastq.gz finished INFO(140621548201792)2022-07-12 17:44:31,068:************************* INFO(140621548201792)2022-07-12 17:44:31,068:deblurring started WARNING(140621548201792)2022-07-12 17:44:31,068:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-22_21_L001_R1_001.fastq.gz WARNING(140621548201792)2022-07-12 17:44:31,068:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-22_21_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(140621548201792)2022-07-12 17:44:31,068:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(140621548201792)2022-07-12 17:44:31,068:deblur worker thread started INFO(140621548201792)2022-07-12 17:44:31,068:processing per sample fasta files INFO(140621548201792)2022-07-12 17:44:31,069:-------------------------------------------------------- INFO(140621548201792)2022-07-12 17:44:31,069:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-22_21_L001_R1_001.fastq.gz INFO(139989471684416)2022-07-12 17:54:44,705:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-32_31_L001_R1_001.fastq.gz.trim INFO(139989471684416)2022-07-12 17:54:45,339:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-32_31_L001_R1_001.fastq.gz.trim.derep INFO(139989471684416)2022-07-12 17:54:46,898:total sequences 32570, passing sequences 32570, failing sequences 0 INFO(139989471684416)2022-07-12 17:54:46,898:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-32_31_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140443028047680)2022-07-12 18:03:07,539:32317 unique sequences left following deblurring INFO(140443028047680)2022-07-12 18:03:09,322:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-26_25_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140443028047680)2022-07-12 18:03:13,293:finished processing file INFO(140443028047680)2022-07-12 18:03:13,342:finished processing per sample fasta files INFO(140443028047680)2022-07-12 18:03:13,342:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-26_25_L001_R1_001.fastq.gz finished INFO(140438718146368)2022-07-12 18:07:30,545:deblurring 29704 sequences INFO(140621548201792)2022-07-12 18:07:54,401:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-22_21_L001_R1_001.fastq.gz.trim INFO(140621548201792)2022-07-12 18:07:55,074:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-22_21_L001_R1_001.fastq.gz.trim.derep INFO(140621548201792)2022-07-12 18:07:56,600:total sequences 32948, passing sequences 32948, failing sequences 0 INFO(140621548201792)2022-07-12 18:07:56,600:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-22_21_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140504788260672)2022-07-12 18:18:53,183:44779 unique sequences left following deblurring INFO(140504788260672)2022-07-12 18:18:56,016:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-08_7_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140504788260672)2022-07-12 18:19:02,834:finished processing file INFO(140504788260672)2022-07-12 18:19:02,928:finished processing per sample fasta files INFO(140504788260672)2022-07-12 18:19:02,928:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-08_7_L001_R1_001.fastq.gz finished INFO(139635526711104)2022-07-12 18:21:34,017:36976 unique sequences left following deblurring INFO(139635526711104)2022-07-12 18:21:36,030:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-16_15_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139635526711104)2022-07-12 18:21:41,788:finished processing file INFO(139635526711104)2022-07-12 18:21:41,827:finished processing per sample fasta files INFO(139635526711104)2022-07-12 18:21:41,827:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-16_15_L001_R1_001.fastq.gz finished INFO(139913984915264)2022-07-12 18:21:42,655:************************* INFO(139913984915264)2022-07-12 18:21:42,655:deblurring started WARNING(139913984915264)2022-07-12 18:21:42,655:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-19_18_L001_R1_001.fastq.gz WARNING(139913984915264)2022-07-12 18:21:42,656:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-19_18_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139913984915264)2022-07-12 18:21:42,656:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139913984915264)2022-07-12 18:21:42,656:deblur worker thread started INFO(139913984915264)2022-07-12 18:21:42,656:processing per sample fasta files INFO(139913984915264)2022-07-12 18:21:42,656:-------------------------------------------------------- INFO(139913984915264)2022-07-12 18:21:42,656:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-19_18_L001_R1_001.fastq.gz INFO(139989471684416)2022-07-12 18:27:21,587:deblurring 32570 sequences INFO(140621548201792)2022-07-12 18:41:10,366:deblurring 32948 sequences INFO(139913984915264)2022-07-12 18:42:28,433:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-19_18_L001_R1_001.fastq.gz.trim INFO(139913984915264)2022-07-12 18:42:29,668:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-19_18_L001_R1_001.fastq.gz.trim.derep INFO(139913984915264)2022-07-12 18:42:31,278:total sequences 32773, passing sequences 32773, failing sequences 0 INFO(139913984915264)2022-07-12 18:42:31,279:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-19_18_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(140438718146368)2022-07-12 18:44:07,169:29704 unique sequences left following deblurring INFO(140438718146368)2022-07-12 18:44:08,953:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-06_5_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140438718146368)2022-07-12 18:44:12,731:finished processing file INFO(140438718146368)2022-07-12 18:44:12,765:finished processing per sample fasta files INFO(140438718146368)2022-07-12 18:44:12,765:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-06_5_L001_R1_001.fastq.gz finished INFO(139885051344704)2022-07-12 18:44:13,779:************************* INFO(139885051344704)2022-07-12 18:44:13,779:deblurring started WARNING(139885051344704)2022-07-12 18:44:13,779:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-07_6_L001_R1_001.fastq.gz WARNING(139885051344704)2022-07-12 18:44:13,779:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-07_6_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139885051344704)2022-07-12 18:44:13,779:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139885051344704)2022-07-12 18:44:13,779:deblur worker thread started INFO(139885051344704)2022-07-12 18:44:13,779:processing per sample fasta files INFO(139885051344704)2022-07-12 18:44:13,779:-------------------------------------------------------- INFO(139885051344704)2022-07-12 18:44:13,779:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-07_6_L001_R1_001.fastq.gz INFO(139885051344704)2022-07-12 19:06:39,804:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-07_6_L001_R1_001.fastq.gz.trim INFO(139885051344704)2022-07-12 19:06:40,443:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-07_6_L001_R1_001.fastq.gz.trim.derep INFO(139885051344704)2022-07-12 19:06:42,099:total sequences 35501, passing sequences 35501, failing sequences 0 INFO(139885051344704)2022-07-12 19:06:42,099:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-07_6_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139989471684416)2022-07-12 19:10:30,194:32570 unique sequences left following deblurring INFO(139989471684416)2022-07-12 19:10:32,119:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-32_31_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139989471684416)2022-07-12 19:10:36,493:finished processing file INFO(139989471684416)2022-07-12 19:10:36,531:finished processing per sample fasta files INFO(139989471684416)2022-07-12 19:10:36,531:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-32_31_L001_R1_001.fastq.gz finished INFO(139808267413312)2022-07-12 19:10:37,344:************************* INFO(139808267413312)2022-07-12 19:10:37,344:deblurring started WARNING(139808267413312)2022-07-12 19:10:37,344:deblur version 1.1.0 workflow started on /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-17_16_L001_R1_001.fastq.gz WARNING(139808267413312)2022-07-12 19:10:37,344:parameters: {'seqs_fp': '/tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-17_16_L001_R1_001.fastq.gz', 'output_dir': '/tmp/tmp3juxj7bc', 'pos_ref_fp': (), 'pos_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/88_otus',), 'neg_ref_fp': (), 'neg_ref_db_fp': ('/tmp/tmp3juxj7bc/deblur_working_dir/artifacts',), 'overwrite': True, 'mean_error': 0.005, 'error_dist': [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005], 'indel_prob': 0.01, 'indel_max': 3, 'trim_length': 280, 'left_trim_length': 0, 'min_reads': 10, 'min_size': 2, 'threads_per_sample': 1, 'keep_tmp_files': True, 'log_level': 2, 'log_file': '/home/ubuntu/C_Bioinformatica04/Proyectos/MICROBIOME/Microbiome_GuevaraCarolina/datos/deblur.log', 'jobs_to_start': 1, 'is_worker_thread': True, 'logger': } INFO(139808267413312)2022-07-12 19:10:37,344:error_dist is : [1, 0.06, 0.02, 0.02, 0.01, 0.005, 0.005, 0.005, 0.001, 0.001, 0.001, 0.0005] INFO(139808267413312)2022-07-12 19:10:37,344:deblur worker thread started INFO(139808267413312)2022-07-12 19:10:37,344:processing per sample fasta files INFO(139808267413312)2022-07-12 19:10:37,344:-------------------------------------------------------- INFO(139808267413312)2022-07-12 19:10:37,344:launch_workflow for file /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-17_16_L001_R1_001.fastq.gz INFO(139913984915264)2022-07-12 19:12:21,921:deblurring 32773 sequences INFO(140621548201792)2022-07-12 19:23:11,929:32948 unique sequences left following deblurring INFO(140621548201792)2022-07-12 19:23:13,664:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-22_21_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(140621548201792)2022-07-12 19:23:17,578:finished processing file INFO(140621548201792)2022-07-12 19:23:17,613:finished processing per sample fasta files INFO(140621548201792)2022-07-12 19:23:17,613:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-22_21_L001_R1_001.fastq.gz finished INFO(139808267413312)2022-07-12 19:31:21,766:dereplicate seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-17_16_L001_R1_001.fastq.gz.trim INFO(139808267413312)2022-07-12 19:31:22,257:remove_artifacts_seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-17_16_L001_R1_001.fastq.gz.trim.derep INFO(139808267413312)2022-07-12 19:31:23,621:total sequences 31160, passing sequences 31160, failing sequences 0 INFO(139808267413312)2022-07-12 19:31:23,621:multiple_sequence_alignment seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-17_16_L001_R1_001.fastq.gz.trim.derep.no_artifacts INFO(139885051344704)2022-07-12 19:49:19,888:deblurring 35501 sequences INFO(139913984915264)2022-07-12 19:54:01,192:32773 unique sequences left following deblurring INFO(139913984915264)2022-07-12 19:54:03,111:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-19_18_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139913984915264)2022-07-12 19:54:07,018:finished processing file INFO(139913984915264)2022-07-12 19:54:07,055:finished processing per sample fasta files INFO(139913984915264)2022-07-12 19:54:07,055:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-19_18_L001_R1_001.fastq.gz finished INFO(139808267413312)2022-07-12 19:58:21,261:deblurring 31160 sequences INFO(139808267413312)2022-07-12 20:33:01,544:31160 unique sequences left following deblurring INFO(139808267413312)2022-07-12 20:33:03,071:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-17_16_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139808267413312)2022-07-12 20:33:06,612:finished processing file INFO(139808267413312)2022-07-12 20:33:06,643:finished processing per sample fasta files INFO(139808267413312)2022-07-12 20:33:06,644:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-17_16_L001_R1_001.fastq.gz finished INFO(139885051344704)2022-07-12 20:38:33,721:35501 unique sequences left following deblurring INFO(139885051344704)2022-07-12 20:38:35,924:remove_chimeras_denovo_from_seqs seqs file /tmp/tmp3juxj7bc/deblur_working_dir/MICRO-8855-07_6_L001_R1_001.fastq.gz.trim.derep.no_artifacts.msa.deblurto working dir /tmp/tmp3juxj7bc/deblur_working_dir INFO(139885051344704)2022-07-12 20:38:40,083:finished processing file INFO(139885051344704)2022-07-12 20:38:40,119:finished processing per sample fasta files INFO(139885051344704)2022-07-12 20:38:40,119:worker thread for /tmp/qiime2-archive-2h28f3gr/5e22a39b-4b61-4916-932a-a711e4bca2ae/data/MICRO-8855-07_6_L001_R1_001.fastq.gz finished INFO(140267207894848)2022-07-12 20:38:40,252:create_otu_table for 36 samples, into output table /tmp/tmp3juxj7bc/all.biom INFO(140267207894848)2022-07-12 20:39:15,161:for output biom table loaded 36 samples, 1134790 unique sequences INFO(140267207894848)2022-07-12 20:39:15,462:keeping 7 (out of 1134790 sequences) with >=10 reads WARNING(140267207894848)2022-07-12 20:39:16,058:removed 29 samples with reads per sample<1 INFO(140267207894848)2022-07-12 20:39:16,193:saved to biom file /tmp/tmp3juxj7bc/all.biom INFO(140267207894848)2022-07-12 20:39:16,193:saved sequence fasta file to /tmp/tmp3juxj7bc/all.seqs.fa INFO(140267207894848)2022-07-12 20:39:16,260:getting 16s sequences from the biom table INFO(140267207894848)2022-07-12 20:39:16,260:remove_artifacts_seqs file /tmp/tmp3juxj7bc/all.seqs.fa INFO(140267207894848)2022-07-12 20:39:18,139:total sequences 7, passing sequences 0, failing sequences 7 WARNING(140267207894848)2022-07-12 20:39:18,139:input file /tmp/tmp3juxj7bc/all.seqs.fa.no_artifacts does not appear to be FASTA or FASTQ INFO(140267207894848)2022-07-12 20:39:18,176:wrote artifact only filtered biom table to /tmp/tmp3juxj7bc/reference-non-hit.biom INFO(140267207894848)2022-07-12 20:39:18,176:saved biom table sequences to fasta file /tmp/tmp3juxj7bc/reference-non-hit.seqs.fa WARNING(140267207894848)2022-07-12 20:39:18,176:removed 7 samples with reads per sample<1 INFO(140267207894848)2022-07-12 20:39:18,180:wrote 16s filtered biom table to /tmp/tmp3juxj7bc/reference-hit.biom INFO(140267207894848)2022-07-12 20:39:18,180:saved biom table sequences to fasta file /tmp/tmp3juxj7bc/reference-hit.seqs.fa INFO(140267207894848)2022-07-12 20:39:18,180:Keeping temp files INFO(140267207894848)2022-07-12 20:39:18,180:deblur workflow finished INFO(140267207894848)2022-07-12 20:39:18,180:output saved to /tmp/tmp3juxj7bc/all.biom INFO(140267207894848)2022-07-12 20:39:18,180:------------------