Running external command line application(s). This may print messages to stdout and/or stderr.
The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist.

Command: run_dada_paired.R /tmp/tmp6_o57z58/forward /tmp/tmp6_o57z58/reverse /tmp/tmp6_o57z58/output.tsv.biom /tmp/tmp6_o57z58/track.tsv /tmp/tmp6_o57z58/filt_f /tmp/tmp6_o57z58/filt_r 247 247 11 10 2.0 2.0 2 consensus 1.0 1 1000000

R version 3.5.1 (2018-07-02) 
Loading required package: Rcpp
DADA2: 1.10.0 / Rcpp: 1.0.3 / RcppParallel: 4.4.4 
1) Filtering ..............................................................................................................................................................................................................................
2) Learning Error Rates
273957296 total bases in 1160836 reads from 5 samples will be used for learning the error rates.
275118132 total bases in 1160836 reads from 5 samples will be used for learning the error rates.
3) Denoise remaining samples ......................Error in dada_uniques(names(derep[[i]]$uniques), unname(derep[[i]]$uniques),  : 
  Memory allocation failed.
Calls: dada -> dada_uniques
Execution halted
Warning message:
system call failed: Cannot allocate memory 
Traceback (most recent call last):
  File "/home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 257, in denoise_paired
    run_commands([cmd])
  File "/home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 36, in run_commands
    subprocess.run(cmd, check=True)
  File "/home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/subprocess.py", line 418, in run
    output=stdout, stderr=stderr)
subprocess.CalledProcessError: Command '['run_dada_paired.R', '/tmp/tmp6_o57z58/forward', '/tmp/tmp6_o57z58/reverse', '/tmp/tmp6_o57z58/output.tsv.biom', '/tmp/tmp6_o57z58/track.tsv', '/tmp/tmp6_o57z58/filt_f', '/tmp/tmp6_o57z58/filt_r', '247', '247', '11', '10', '2.0', '2.0', '2', 'consensus', '1.0', '1', '1000000']' returned non-zero exit status 1.

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/site-packages/q2cli/commands.py", line 328, in __call__
    results = action(**arguments)
  File "</home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/site-packages/decorator.py:decorator-gen-455>", line 2, in denoise_paired
  File "/home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/site-packages/qiime2/sdk/action.py", line 245, in bound_callable
    output_types, provenance)
  File "/home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/site-packages/qiime2/sdk/action.py", line 390, in _callable_executor_
    output_views = self._callable(**view_args)
  File "/home/qiime2/miniconda/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 272, in denoise_paired
    " and stderr to learn more." % e.returncode)
Exception: An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.