This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_01_0_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_01_227_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_01_0_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_01_227_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.17 s (11127 µs/read; 0.01 M reads/minute). === Summary === Total read pairs processed: 15 Read 1 with adapter: 14 (93.3%) Read 2 with adapter: 13 (86.7%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 2 (13.3%) Pairs written (passing filters): 13 (86.7%) Total basepairs processed: 5,278 bp Read 1: 3,036 bp Read 2: 2,242 bp Total written (filtered): 793 bp (15.0%) Read 1: 403 bp Read 2: 390 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 14 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 92.9% G: 0.0% T: 7.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 20 2 0.0 6 2 134 1 0.0 6 1 154 1 0.0 6 0 0 0 0 0 0 1 178 1 0.0 6 1 187 1 0.0 6 1 190 1 0.0 6 1 201 1 0.0 6 1 205 1 0.0 6 1 207 1 0.0 6 1 209 1 0.0 6 1 215 1 0.0 6 1 240 1 0.0 6 1 265 1 0.0 6 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 0 times === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 13 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 20 3 0.0 6 3 61 2 0.0 6 2 125 1 0.0 6 1 132 1 0.0 6 1 150 1 0.0 6 1 155 1 0.0 6 1 168 1 0.0 6 1 190 1 0.0 6 1 199 1 0.0 6 1 228 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 0 times This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_02_1_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_02_228_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_02_1_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_02_228_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.66 s (37 µs/read; 1.61 M reads/minute). === Summary === Total read pairs processed: 71,111 Read 1 with adapter: 71,102 (100.0%) Read 2 with adapter: 71,111 (100.0%) == Read fate breakdown == Pairs that were too short: 42 (0.1%) Pairs discarded as untrimmed: 9 (0.0%) Pairs written (passing filters): 71,060 (99.9%) Total basepairs processed: 30,443,699 bp Read 1: 17,144,022 bp Read 2: 13,299,677 bp Total written (filtered): 4,505,065 bp (14.8%) Read 1: 2,274,236 bp Read 2: 2,230,829 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 69486 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 1111.1 0 0 3 4 2 277.8 1 1 1 5 1 69.4 1 1 6 2 17.4 1 0 0 2 7 3 4.3 2 2 0 1 9 4 0.3 2 4 15 2 0.0 4 2 16 5 0.0 4 3 2 17 4 0.0 5 4 18 1 0.0 5 1 19 3 0.0 5 2 1 20 92 0.0 6 89 3 21 42 0.0 6 41 1 22 55 0.0 6 52 3 23 39 0.0 6 38 1 24 36 0.0 6 34 2 25 21 0.0 6 20 1 26 19 0.0 6 17 2 27 6 0.0 6 6 28 7 0.0 6 7 29 6 0.0 6 6 30 10 0.0 6 10 31 11 0.0 6 11 32 8 0.0 6 8 33 5 0.0 6 5 34 8 0.0 6 8 35 2 0.0 6 2 36 7 0.0 6 7 37 6 0.0 6 6 38 6 0.0 6 6 39 7 0.0 6 7 40 5 0.0 6 5 41 13 0.0 6 13 42 6 0.0 6 6 43 10 0.0 6 9 1 44 7 0.0 6 7 45 6 0.0 6 6 46 5 0.0 6 5 47 4 0.0 6 4 48 5 0.0 6 4 1 49 11 0.0 6 9 2 50 8 0.0 6 6 2 51 10 0.0 6 10 52 8 0.0 6 7 1 53 9 0.0 6 9 54 3 0.0 6 2 1 55 6 0.0 6 6 57 4 0.0 6 4 58 2 0.0 6 2 59 3 0.0 6 2 1 60 11 0.0 6 9 1 1 61 4 0.0 6 4 62 4 0.0 6 4 63 10 0.0 6 10 64 6 0.0 6 6 65 2 0.0 6 2 66 5 0.0 6 5 67 7 0.0 6 7 68 10 0.0 6 10 69 9 0.0 6 8 1 70 7 0.0 6 7 71 16 0.0 6 16 72 14 0.0 6 14 73 12 0.0 6 12 74 10 0.0 6 9 0 1 75 10 0.0 6 9 1 76 12 0.0 6 12 77 10 0.0 6 9 1 78 9 0.0 6 9 79 10 0.0 6 10 80 12 0.0 6 11 0 1 81 7 0.0 6 7 82 10 0.0 6 10 83 10 0.0 6 9 1 84 13 0.0 6 11 1 1 85 13 0.0 6 13 86 16 0.0 6 16 87 13 0.0 6 12 1 88 14 0.0 6 14 89 8 0.0 6 8 90 15 0.0 6 14 1 91 12 0.0 6 10 1 1 92 9 0.0 6 7 2 93 6 0.0 6 6 94 10 0.0 6 10 95 12 0.0 6 11 1 96 11 0.0 6 11 97 5 0.0 6 5 98 14 0.0 6 14 99 9 0.0 6 9 100 13 0.0 6 13 101 13 0.0 6 12 1 102 18 0.0 6 17 1 103 21 0.0 6 20 1 104 19 0.0 6 17 1 1 105 19 0.0 6 18 1 106 20 0.0 6 17 2 1 107 18 0.0 6 18 108 22 0.0 6 21 1 109 18 0.0 6 18 110 24 0.0 6 24 111 22 0.0 6 22 112 19 0.0 6 18 1 113 18 0.0 6 17 0 0 0 0 1 114 26 0.0 6 25 1 115 16 0.0 6 15 1 116 26 0.0 6 25 0 0 0 0 1 117 20 0.0 6 19 1 118 19 0.0 6 18 1 119 29 0.0 6 28 1 120 29 0.0 6 28 0 0 1 121 32 0.0 6 30 1 1 122 20 0.0 6 19 1 123 27 0.0 6 25 2 124 29 0.0 6 28 1 125 25 0.0 6 24 0 1 126 29 0.0 6 27 2 127 31 0.0 6 30 0 0 0 0 0 1 128 32 0.0 6 31 0 0 0 0 0 1 129 39 0.0 6 39 130 39 0.0 6 39 131 37 0.0 6 36 0 1 132 33 0.0 6 32 1 133 33 0.0 6 31 2 134 47 0.0 6 46 1 135 27 0.0 6 27 136 44 0.0 6 43 1 137 37 0.0 6 37 138 48 0.0 6 47 1 139 52 0.0 6 51 0 0 0 0 1 140 57 0.0 6 56 1 141 60 0.0 6 59 1 142 177 0.0 6 177 143 289 0.0 6 289 144 326 0.0 6 323 3 145 300 0.0 6 299 1 146 287 0.0 6 286 1 147 397 0.0 6 394 3 148 356 0.0 6 356 149 396 0.0 6 394 2 150 401 0.0 6 401 151 364 0.0 6 364 152 474 0.0 6 471 3 153 307 0.0 6 305 2 154 221 0.0 6 217 3 0 1 155 251 0.0 6 251 156 253 0.0 6 250 2 0 0 0 1 157 236 0.0 6 236 158 183 0.0 6 182 1 159 263 0.0 6 261 1 1 160 252 0.0 6 252 161 271 0.0 6 269 2 162 269 0.0 6 265 4 163 267 0.0 6 264 2 1 164 276 0.0 6 275 0 0 0 1 165 267 0.0 6 265 0 0 1 1 166 231 0.0 6 230 0 1 167 241 0.0 6 238 2 1 168 264 0.0 6 262 1 1 169 280 0.0 6 278 2 170 246 0.0 6 244 2 171 272 0.0 6 269 2 0 0 0 1 172 269 0.0 6 268 1 173 249 0.0 6 247 2 174 323 0.0 6 320 2 1 175 275 0.0 6 274 1 176 327 0.0 6 326 1 177 348 0.0 6 343 3 1 1 178 257 0.0 6 256 1 179 313 0.0 6 312 1 180 705 0.0 6 702 3 181 366 0.0 6 363 2 0 0 1 182 331 0.0 6 331 183 381 0.0 6 379 1 0 0 1 184 339 0.0 6 335 2 0 1 1 185 349 0.0 6 346 2 1 186 369 0.0 6 366 2 0 0 1 187 456 0.0 6 451 5 188 398 0.0 6 397 1 189 360 0.0 6 358 2 190 373 0.0 6 369 4 191 370 0.0 6 365 4 0 1 192 489 0.0 6 485 4 193 474 0.0 6 472 1 0 1 194 547 0.0 6 544 3 195 597 0.0 6 593 4 196 651 0.0 6 642 8 0 0 1 197 745 0.0 6 739 6 198 873 0.0 6 866 6 0 0 0 1 199 897 0.0 6 892 4 0 0 0 0 1 200 714 0.0 6 707 5 0 1 0 1 201 685 0.0 6 681 4 202 685 0.0 6 684 1 203 624 0.0 6 615 7 0 1 0 0 1 204 884 0.0 6 877 7 205 719 0.0 6 715 4 206 786 0.0 6 782 4 207 1008 0.0 6 998 8 0 1 1 208 707 0.0 6 700 6 1 209 866 0.0 6 856 8 0 1 0 0 1 210 727 0.0 6 721 5 0 0 0 0 1 211 768 0.0 6 762 6 212 875 0.0 6 871 4 213 949 0.0 6 940 8 1 214 906 0.0 6 900 5 0 0 0 0 1 215 986 0.0 6 983 3 216 1241 0.0 6 1233 7 0 0 0 1 217 916 0.0 6 914 2 218 953 0.0 6 949 4 219 1012 0.0 6 1007 5 220 889 0.0 6 886 3 221 743 0.0 6 738 4 0 1 222 782 0.0 6 778 3 0 0 0 0 1 223 730 0.0 6 726 4 224 807 0.0 6 804 3 225 822 0.0 6 820 2 226 964 0.0 6 961 2 0 1 227 931 0.0 6 922 9 228 770 0.0 6 768 1 0 1 229 689 0.0 6 685 4 230 712 0.0 6 709 2 0 1 231 746 0.0 6 744 2 232 742 0.0 6 739 3 233 724 0.0 6 720 4 234 776 0.0 6 772 4 235 637 0.0 6 635 1 1 236 539 0.0 6 537 2 237 592 0.0 6 587 3 2 238 568 0.0 6 565 2 0 0 0 0 1 239 573 0.0 6 566 7 240 616 0.0 6 615 1 241 555 0.0 6 550 4 1 242 538 0.0 6 537 1 243 566 0.0 6 563 3 244 491 0.0 6 489 2 245 400 0.0 6 397 3 246 397 0.0 6 394 2 1 247 352 0.0 6 351 1 248 361 0.0 6 361 249 381 0.0 6 379 2 250 357 0.0 6 355 1 0 0 0 0 1 251 309 0.0 6 308 0 0 1 252 321 0.0 6 320 1 253 259 0.0 6 256 2 0 0 0 1 254 308 0.0 6 308 255 298 0.0 6 298 256 242 0.0 6 241 1 257 243 0.0 6 243 258 269 0.0 6 267 1 0 0 0 1 259 260 0.0 6 258 2 260 320 0.0 6 320 261 232 0.0 6 231 1 262 206 0.0 6 205 1 263 199 0.0 6 197 2 264 209 0.0 6 209 265 198 0.0 6 198 266 176 0.0 6 176 267 190 0.0 6 188 2 268 131 0.0 6 130 0 1 269 173 0.0 6 172 1 270 190 0.0 6 190 271 146 0.0 6 145 1 272 139 0.0 6 137 1 1 273 132 0.0 6 131 1 274 163 0.0 6 159 4 275 162 0.0 6 161 0 0 0 0 0 1 276 225 0.0 6 225 277 276 0.0 6 275 0 0 0 0 0 1 278 387 0.0 6 383 4 279 1120 0.0 6 1107 12 1 280 2030 0.0 6 2011 17 1 0 0 1 281 7 0.0 6 7 282 4 0.0 6 3 1 289 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1616 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 4 0.0 6 0 4 62 113 0.0 6 111 2 64 3 0.0 6 2 1 69 2 0.0 6 2 70 2 0.0 6 2 71 3 0.0 6 3 72 11 0.0 6 11 73 5 0.0 6 5 74 9 0.0 6 8 1 75 14 0.0 6 14 76 12 0.0 6 12 77 6 0.0 6 6 78 13 0.0 6 13 79 8 0.0 6 8 80 12 0.0 6 12 81 17 0.0 6 17 82 6 0.0 6 6 83 12 0.0 6 12 84 5 0.0 6 5 85 3 0.0 6 3 86 3 0.0 6 3 87 9 0.0 6 9 88 6 0.0 6 6 89 4 0.0 6 4 90 12 0.0 6 12 91 4 0.0 6 4 92 5 0.0 6 5 93 7 0.0 6 7 94 3 0.0 6 3 95 2 0.0 6 2 96 9 0.0 6 9 97 4 0.0 6 4 98 8 0.0 6 8 99 5 0.0 6 5 100 5 0.0 6 5 101 8 0.0 6 8 102 9 0.0 6 9 103 4 0.0 6 4 104 5 0.0 6 5 105 7 0.0 6 7 106 4 0.0 6 4 107 7 0.0 6 7 108 5 0.0 6 5 109 22 0.0 6 22 110 6 0.0 6 6 111 4 0.0 6 4 112 4 0.0 6 4 113 2 0.0 6 2 114 6 0.0 6 6 115 5 0.0 6 5 116 7 0.0 6 7 117 7 0.0 6 7 118 6 0.0 6 6 119 6 0.0 6 6 120 3 0.0 6 3 121 4 0.0 6 4 122 5 0.0 6 5 123 5 0.0 6 5 124 11 0.0 6 11 125 7 0.0 6 7 126 6 0.0 6 6 127 11 0.0 6 11 128 2 0.0 6 2 129 2 0.0 6 2 130 6 0.0 6 6 131 5 0.0 6 5 132 11 0.0 6 11 133 10 0.0 6 10 134 3 0.0 6 3 135 5 0.0 6 5 136 9 0.0 6 9 137 7 0.0 6 7 138 7 0.0 6 7 139 7 0.0 6 7 140 6 0.0 6 6 141 8 0.0 6 8 142 5 0.0 6 4 1 143 13 0.0 6 13 144 7 0.0 6 7 145 7 0.0 6 7 146 4 0.0 6 4 147 10 0.0 6 10 148 8 0.0 6 8 149 14 0.0 6 14 150 7 0.0 6 7 151 11 0.0 6 11 152 6 0.0 6 6 153 7 0.0 6 7 154 8 0.0 6 8 155 8 0.0 6 8 156 13 0.0 6 13 157 4 0.0 6 4 158 1 0.0 6 1 159 7 0.0 6 7 160 10 0.0 6 10 161 12 0.0 6 12 162 17 0.0 6 17 163 19 0.0 6 19 164 11 0.0 6 11 165 7 0.0 6 7 166 9 0.0 6 9 167 10 0.0 6 10 168 13 0.0 6 13 169 13 0.0 6 13 170 7 0.0 6 7 171 6 0.0 6 6 172 8 0.0 6 8 173 12 0.0 6 12 174 9 0.0 6 9 175 12 0.0 6 12 176 13 0.0 6 13 177 10 0.0 6 10 178 11 0.0 6 11 179 7 0.0 6 7 180 5 0.0 6 5 181 13 0.0 6 13 182 6 0.0 6 6 183 10 0.0 6 10 184 12 0.0 6 12 185 10 0.0 6 10 186 9 0.0 6 9 187 15 0.0 6 15 188 11 0.0 6 11 189 12 0.0 6 12 190 9 0.0 6 9 191 11 0.0 6 11 192 10 0.0 6 10 193 11 0.0 6 11 194 13 0.0 6 13 195 4 0.0 6 4 196 11 0.0 6 11 197 11 0.0 6 11 198 13 0.0 6 13 199 10 0.0 6 10 200 19 0.0 6 19 201 11 0.0 6 11 202 16 0.0 6 16 203 7 0.0 6 7 204 9 0.0 6 9 205 7 0.0 6 7 206 7 0.0 6 7 207 10 0.0 6 10 208 6 0.0 6 6 209 5 0.0 6 5 210 8 0.0 6 8 211 11 0.0 6 11 212 5 0.0 6 5 213 8 0.0 6 8 214 7 0.0 6 7 215 6 0.0 6 6 216 9 0.0 6 9 217 10 0.0 6 10 218 9 0.0 6 9 219 4 0.0 6 4 220 5 0.0 6 5 221 7 0.0 6 7 222 2 0.0 6 2 223 10 0.0 6 10 224 5 0.0 6 5 225 7 0.0 6 7 226 14 0.0 6 14 227 9 0.0 6 9 228 8 0.0 6 8 229 9 0.0 6 9 230 10 0.0 6 10 231 5 0.0 6 5 232 4 0.0 6 4 233 8 0.0 6 8 234 6 0.0 6 6 235 5 0.0 6 5 236 7 0.0 6 7 237 6 0.0 6 6 238 5 0.0 6 5 239 6 0.0 6 6 240 3 0.0 6 3 241 11 0.0 6 11 242 5 0.0 6 5 243 4 0.0 6 4 244 7 0.0 6 7 245 4 0.0 6 4 246 7 0.0 6 7 247 8 0.0 6 8 248 3 0.0 6 3 249 2 0.0 6 2 250 3 0.0 6 3 251 4 0.0 6 4 252 8 0.0 6 8 253 5 0.0 6 5 254 2 0.0 6 2 255 1 0.0 6 1 256 3 0.0 6 3 257 2 0.0 6 2 258 1 0.0 6 1 259 7 0.0 6 7 260 5 0.0 6 5 261 2 0.0 6 2 262 2 0.0 6 2 263 3 0.0 6 3 264 2 0.0 6 2 265 1 0.0 6 1 267 1 0.0 6 1 268 3 0.0 6 3 269 1 0.0 6 1 270 1 0.0 6 1 274 1 0.0 6 1 275 2 0.0 6 2 278 1 0.0 6 1 279 2 0.0 6 2 280 2 0.0 6 2 281 1 0.0 6 1 283 1 0.0 6 1 285 1 0.0 6 1 286 1 0.0 6 1 288 1 0.0 6 1 289 1 0.0 6 1 290 2 0.0 6 2 292 1 0.0 6 1 294 1 0.0 6 1 297 2 0.0 6 2 299 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 69872 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 277.8 1 1 6 1 17.4 1 1 10 8 0.1 3 8 11 3 0.0 3 3 12 4 0.0 3 4 13 7 0.0 3 6 1 14 9 0.0 4 8 1 15 5 0.0 4 3 1 1 16 8 0.0 4 7 1 17 56 0.0 5 52 3 1 18 24 0.0 5 23 1 19 22 0.0 5 19 2 1 20 80 0.0 6 77 3 21 44 0.0 6 43 1 22 28 0.0 6 27 1 23 14 0.0 6 14 24 16 0.0 6 15 1 25 20 0.0 6 19 1 26 15 0.0 6 14 1 27 13 0.0 6 11 2 28 15 0.0 6 15 29 8 0.0 6 8 30 20 0.0 6 19 1 31 19 0.0 6 16 3 32 21 0.0 6 20 1 33 16 0.0 6 14 2 34 15 0.0 6 15 35 15 0.0 6 11 4 36 15 0.0 6 14 0 1 37 9 0.0 6 9 38 16 0.0 6 12 3 0 0 1 39 8 0.0 6 6 2 40 14 0.0 6 14 41 17 0.0 6 16 1 42 10 0.0 6 10 43 17 0.0 6 15 2 44 17 0.0 6 14 3 45 15 0.0 6 14 1 46 28 0.0 6 26 2 47 14 0.0 6 13 1 48 19 0.0 6 17 1 0 0 1 49 19 0.0 6 17 2 50 24 0.0 6 24 51 20 0.0 6 20 52 33 0.0 6 31 1 1 53 34 0.0 6 30 4 54 22 0.0 6 18 4 55 33 0.0 6 31 2 56 21 0.0 6 19 2 57 24 0.0 6 24 58 34 0.0 6 31 3 59 38 0.0 6 34 4 60 34 0.0 6 30 0 2 1 1 61 27 0.0 6 23 3 0 0 0 1 62 42 0.0 6 41 0 1 63 25 0.0 6 23 0 1 0 0 0 1 64 47 0.0 6 43 3 1 65 40 0.0 6 38 2 66 43 0.0 6 43 67 68 0.0 6 63 3 1 1 68 137 0.0 6 130 4 0 1 1 0 1 69 69 0.0 6 65 4 70 76 0.0 6 72 4 71 40 0.0 6 35 2 2 0 0 1 72 81 0.0 6 74 6 0 0 0 0 1 73 36 0.0 6 36 74 56 0.0 6 52 4 75 42 0.0 6 38 4 76 34 0.0 6 33 0 0 1 77 34 0.0 6 32 2 78 42 0.0 6 35 5 1 0 0 0 1 79 50 0.0 6 46 2 1 0 1 80 49 0.0 6 47 2 81 72 0.0 6 69 2 0 0 1 82 58 0.0 6 56 2 83 74 0.0 6 69 3 0 1 0 1 84 89 0.0 6 86 2 0 0 0 0 1 85 54 0.0 6 52 2 86 59 0.0 6 58 0 0 0 0 1 87 47 0.0 6 44 2 0 0 0 1 88 69 0.0 6 64 4 1 89 60 0.0 6 56 4 90 66 0.0 6 64 2 91 90 0.0 6 86 4 92 60 0.0 6 59 1 93 73 0.0 6 69 4 94 91 0.0 6 84 6 0 0 1 95 93 0.0 6 90 3 96 93 0.0 6 92 1 97 102 0.0 6 95 7 98 97 0.0 6 97 99 75 0.0 6 72 3 100 71 0.0 6 68 3 101 108 0.0 6 103 2 1 0 2 102 104 0.0 6 100 4 103 111 0.0 6 110 1 104 130 0.0 6 122 5 0 1 0 2 105 210 0.0 6 204 5 0 0 0 0 1 106 146 0.0 6 140 6 107 109 0.0 6 102 5 1 1 108 131 0.0 6 126 5 109 121 0.0 6 114 4 1 0 2 110 145 0.0 6 142 2 0 0 1 111 160 0.0 6 156 3 0 1 112 152 0.0 6 145 6 0 1 113 192 0.0 6 188 3 0 0 0 0 1 114 169 0.0 6 166 2 0 0 0 1 115 178 0.0 6 171 7 116 187 0.0 6 186 1 117 199 0.0 6 190 6 0 0 0 0 3 118 219 0.0 6 214 5 119 212 0.0 6 207 4 0 0 0 0 1 120 193 0.0 6 189 3 0 0 0 1 121 205 0.0 6 197 7 0 1 122 210 0.0 6 204 5 0 0 0 0 1 123 222 0.0 6 208 10 0 1 0 1 2 124 266 0.0 6 262 3 1 125 260 0.0 6 253 4 0 3 126 321 0.0 6 313 5 0 1 1 0 1 127 339 0.0 6 332 4 1 1 1 128 351 0.0 6 338 10 1 1 0 1 129 396 0.0 6 380 12 1 1 1 1 130 447 0.0 6 436 9 0 0 1 1 131 437 0.0 6 424 13 132 447 0.0 6 439 7 1 133 498 0.0 6 487 7 1 0 0 2 1 134 509 0.0 6 495 12 0 1 1 135 537 0.0 6 521 13 0 2 0 0 1 136 561 0.0 6 546 10 0 0 2 1 2 137 503 0.0 6 491 11 1 138 486 0.0 6 481 5 139 616 0.0 6 604 8 1 1 1 1 140 748 0.0 6 741 5 0 0 1 1 141 792 0.0 6 784 6 0 0 0 2 142 815 0.0 6 803 11 0 0 1 143 898 0.0 6 884 12 0 0 1 0 1 144 838 0.0 6 820 12 1 2 1 1 1 145 1034 0.0 6 1020 12 1 1 146 1026 0.0 6 1010 12 0 1 2 1 147 1100 0.0 6 1090 9 1 148 1177 0.0 6 1151 22 0 2 0 1 1 149 1330 0.0 6 1309 15 1 2 1 0 2 150 1325 0.0 6 1301 17 1 1 2 0 3 151 1586 0.0 6 1558 23 3 1 0 0 1 152 1273 0.0 6 1252 19 0 0 1 1 153 1471 0.0 6 1446 19 1 0 1 4 154 1407 0.0 6 1389 17 0 1 155 1184 0.0 6 1159 19 1 2 1 2 156 1225 0.0 6 1212 11 2 157 1192 0.0 6 1175 15 0 0 1 1 158 1299 0.0 6 1280 16 1 1 0 1 159 1194 0.0 6 1175 19 160 1115 0.0 6 1098 13 1 2 0 1 161 1040 0.0 6 1022 15 0 1 0 1 1 162 981 0.0 6 972 6 0 0 0 1 2 163 858 0.0 6 848 8 0 1 0 1 164 946 0.0 6 935 10 0 0 0 1 165 935 0.0 6 927 8 166 866 0.0 6 853 11 0 0 0 0 2 167 1067 0.0 6 1046 14 3 0 4 168 850 0.0 6 839 7 2 0 1 0 1 169 1043 0.0 6 1027 12 3 0 0 0 1 170 903 0.0 6 891 10 0 0 1 0 1 171 920 0.0 6 908 9 0 1 1 0 1 172 992 0.0 6 984 7 1 173 1006 0.0 6 993 11 0 0 1 0 1 174 992 0.0 6 984 4 1 1 0 0 2 175 904 0.0 6 893 9 0 0 1 0 1 176 924 0.0 6 911 10 2 0 0 0 1 177 1071 0.0 6 1063 6 1 0 0 0 1 178 722 0.0 6 714 6 0 1 0 1 179 754 0.0 6 749 4 0 1 180 626 0.0 6 618 7 1 181 584 0.0 6 577 7 182 547 0.0 6 538 7 2 183 551 0.0 6 545 6 184 809 0.0 6 803 5 0 0 0 1 185 966 0.0 6 958 7 0 1 186 845 0.0 6 839 4 1 0 0 0 1 187 546 0.0 6 541 3 1 0 0 0 1 188 566 0.0 6 558 7 0 0 1 189 551 0.0 6 543 7 0 1 190 513 0.0 6 508 4 0 0 0 1 191 475 0.0 6 472 3 192 501 0.0 6 497 4 193 357 0.0 6 352 3 1 1 194 331 0.0 6 323 6 1 1 195 355 0.0 6 351 3 1 196 293 0.0 6 293 197 370 0.0 6 365 5 198 314 0.0 6 312 2 199 299 0.0 6 296 3 200 245 0.0 6 243 0 0 0 0 2 201 247 0.0 6 245 2 202 188 0.0 6 187 1 203 191 0.0 6 190 1 204 187 0.0 6 186 1 205 154 0.0 6 153 1 206 149 0.0 6 149 207 146 0.0 6 144 2 208 148 0.0 6 147 1 209 121 0.0 6 120 1 210 125 0.0 6 124 1 211 81 0.0 6 81 212 104 0.0 6 104 213 117 0.0 6 117 214 86 0.0 6 85 1 215 128 0.0 6 127 1 216 109 0.0 6 108 1 217 69 0.0 6 66 2 0 1 218 61 0.0 6 61 219 56 0.0 6 56 220 41 0.0 6 41 221 29 0.0 6 28 1 222 62 0.0 6 61 1 223 51 0.0 6 51 224 34 0.0 6 34 225 31 0.0 6 31 226 20 0.0 6 19 1 227 23 0.0 6 22 1 228 22 0.0 6 21 1 229 29 0.0 6 29 230 18 0.0 6 18 231 14 0.0 6 14 232 17 0.0 6 17 233 18 0.0 6 17 1 234 11 0.0 6 11 235 9 0.0 6 9 236 4 0.0 6 4 237 2 0.0 6 2 238 2 0.0 6 2 239 3 0.0 6 3 240 5 0.0 6 5 241 3 0.0 6 3 242 8 0.0 6 8 243 3 0.0 6 3 244 4 0.0 6 4 245 3 0.0 6 3 246 1 0.0 6 1 247 1 0.0 6 1 248 2 0.0 6 2 249 2 0.0 6 2 252 1 0.0 6 1 254 1 0.0 6 1 277 1 0.0 6 1 278 6 0.0 6 6 279 11 0.0 6 11 280 106 0.0 6 105 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1239 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 3 73 1111.1 0 73 50 1 0.0 6 0 1 52 1 0.0 6 1 54 1 0.0 6 1 55 2 0.0 6 2 57 2 0.0 6 2 58 2 0.0 6 2 59 1 0.0 6 1 60 1 0.0 6 1 62 4 0.0 6 4 66 1 0.0 6 1 67 1 0.0 6 1 68 2 0.0 6 2 69 2 0.0 6 2 70 1 0.0 6 1 71 12 0.0 6 12 72 8 0.0 6 8 73 6 0.0 6 6 74 7 0.0 6 7 75 11 0.0 6 11 76 16 0.0 6 16 77 12 0.0 6 12 78 10 0.0 6 10 79 19 0.0 6 18 1 80 18 0.0 6 18 81 18 0.0 6 18 82 7 0.0 6 7 83 3 0.0 6 3 84 6 0.0 6 6 85 6 0.0 6 6 86 10 0.0 6 10 87 4 0.0 6 4 88 7 0.0 6 7 89 7 0.0 6 7 90 3 0.0 6 3 91 9 0.0 6 9 92 10 0.0 6 10 93 3 0.0 6 3 94 6 0.0 6 6 95 8 0.0 6 8 96 5 0.0 6 5 97 3 0.0 6 3 98 6 0.0 6 6 99 9 0.0 6 9 100 4 0.0 6 4 101 2 0.0 6 2 102 4 0.0 6 4 103 10 0.0 6 10 104 3 0.0 6 3 105 4 0.0 6 4 106 6 0.0 6 6 107 7 0.0 6 7 108 3 0.0 6 2 0 0 0 1 109 23 0.0 6 23 110 6 0.0 6 6 111 5 0.0 6 5 112 10 0.0 6 10 113 3 0.0 6 3 114 5 0.0 6 5 115 7 0.0 6 7 116 10 0.0 6 10 117 2 0.0 6 2 118 11 0.0 6 10 0 0 0 1 119 4 0.0 6 4 120 3 0.0 6 3 121 4 0.0 6 4 122 10 0.0 6 10 123 2 0.0 6 2 124 13 0.0 6 13 125 7 0.0 6 7 126 5 0.0 6 5 127 7 0.0 6 7 128 4 0.0 6 4 129 4 0.0 6 3 0 0 0 1 130 12 0.0 6 12 131 6 0.0 6 6 132 9 0.0 6 9 133 17 0.0 6 17 134 6 0.0 6 6 135 4 0.0 6 4 136 11 0.0 6 11 137 7 0.0 6 7 138 16 0.0 6 16 139 8 0.0 6 8 140 9 0.0 6 9 141 7 0.0 6 7 142 7 0.0 6 7 143 14 0.0 6 14 144 7 0.0 6 7 145 10 0.0 6 10 146 6 0.0 6 6 147 10 0.0 6 10 148 8 0.0 6 8 149 9 0.0 6 9 150 13 0.0 6 13 151 17 0.0 6 17 152 9 0.0 6 9 153 10 0.0 6 10 154 7 0.0 6 7 155 4 0.0 6 4 156 7 0.0 6 7 157 14 0.0 6 14 158 8 0.0 6 8 159 5 0.0 6 5 160 9 0.0 6 9 161 14 0.0 6 13 0 0 0 1 162 21 0.0 6 21 163 12 0.0 6 12 164 4 0.0 6 4 165 12 0.0 6 12 166 21 0.0 6 21 167 10 0.0 6 10 168 6 0.0 6 6 169 14 0.0 6 14 170 11 0.0 6 11 171 10 0.0 6 9 0 0 1 172 13 0.0 6 13 173 6 0.0 6 6 174 14 0.0 6 14 175 9 0.0 6 9 176 12 0.0 6 12 177 9 0.0 6 9 178 10 0.0 6 10 179 5 0.0 6 4 1 180 6 0.0 6 6 181 9 0.0 6 9 182 9 0.0 6 9 183 8 0.0 6 8 184 9 0.0 6 9 185 4 0.0 6 4 186 4 0.0 6 4 187 13 0.0 6 13 188 4 0.0 6 4 189 4 0.0 6 4 190 9 0.0 6 9 191 8 0.0 6 8 192 9 0.0 6 8 1 193 10 0.0 6 10 194 3 0.0 6 3 195 5 0.0 6 5 196 6 0.0 6 6 197 3 0.0 6 3 199 5 0.0 6 5 200 5 0.0 6 5 201 4 0.0 6 4 202 6 0.0 6 6 203 5 0.0 6 5 204 5 0.0 6 5 205 6 0.0 6 6 206 2 0.0 6 2 207 1 0.0 6 1 208 5 0.0 6 5 209 4 0.0 6 4 210 4 0.0 6 4 211 2 0.0 6 2 212 3 0.0 6 3 213 1 0.0 6 1 214 2 0.0 6 2 215 2 0.0 6 2 217 4 0.0 6 4 218 5 0.0 6 5 220 2 0.0 6 2 222 2 0.0 6 2 223 1 0.0 6 1 225 1 0.0 6 1 227 3 0.0 6 3 231 1 0.0 6 1 233 1 0.0 6 1 236 1 0.0 6 1 237 1 0.0 6 1 243 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_03_2_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_03_229_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_03_2_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_03_229_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.54 s (35 µs/read; 1.72 M reads/minute). === Summary === Total read pairs processed: 130,446 Read 1 with adapter: 130,435 (100.0%) Read 2 with adapter: 130,349 (99.9%) == Read fate breakdown == Pairs that were too short: 4 (0.0%) Pairs discarded as untrimmed: 108 (0.1%) Pairs written (passing filters): 130,334 (99.9%) Total basepairs processed: 65,852,588 bp Read 1: 37,426,971 bp Read 2: 28,425,617 bp Total written (filtered): 8,226,710 bp (12.5%) Read 1: 4,050,905 bp Read 2: 4,175,805 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 130224 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 2038.2 0 1 1 4 3 509.6 1 0 3 5 1 127.4 1 1 6 3 31.8 1 0 0 3 7 2 8.0 2 0 0 2 9 2 0.5 2 2 13 1 0.0 3 1 15 2 0.0 4 2 16 1 0.0 4 1 20 36 0.0 6 36 21 30 0.0 6 29 1 22 17 0.0 6 16 0 1 23 29 0.0 6 29 24 21 0.0 6 21 25 3 0.0 6 3 26 28 0.0 6 27 1 27 4 0.0 6 4 28 17 0.0 6 16 0 1 29 8 0.0 6 8 30 7 0.0 6 7 31 2 0.0 6 2 33 12 0.0 6 8 3 0 1 34 5 0.0 6 5 35 5 0.0 6 4 1 36 2 0.0 6 2 37 2 0.0 6 2 38 4 0.0 6 4 39 12 0.0 6 12 40 10 0.0 6 9 1 41 5 0.0 6 4 1 42 3 0.0 6 2 1 43 2 0.0 6 1 1 44 18 0.0 6 18 45 5 0.0 6 5 46 3 0.0 6 3 47 4 0.0 6 3 1 48 4 0.0 6 4 49 9 0.0 6 8 1 50 6 0.0 6 6 52 5 0.0 6 5 53 12 0.0 6 10 2 54 7 0.0 6 6 1 55 5 0.0 6 5 56 12 0.0 6 12 57 4 0.0 6 4 58 1 0.0 6 1 59 7 0.0 6 7 60 5 0.0 6 5 61 4 0.0 6 4 62 14 0.0 6 13 1 63 2 0.0 6 2 64 1 0.0 6 1 65 6 0.0 6 6 66 2 0.0 6 2 67 9 0.0 6 9 68 3 0.0 6 3 69 5 0.0 6 4 1 70 4 0.0 6 4 71 2 0.0 6 1 1 72 2 0.0 6 2 73 1 0.0 6 1 74 2 0.0 6 2 75 4 0.0 6 4 76 10 0.0 6 10 77 7 0.0 6 7 78 5 0.0 6 5 80 6 0.0 6 6 81 7 0.0 6 7 82 4 0.0 6 4 83 3 0.0 6 3 84 6 0.0 6 5 1 85 5 0.0 6 4 1 86 10 0.0 6 10 87 9 0.0 6 8 1 88 3 0.0 6 2 1 89 11 0.0 6 10 1 90 10 0.0 6 8 1 1 91 5 0.0 6 5 92 12 0.0 6 11 1 93 10 0.0 6 10 94 5 0.0 6 4 1 95 6 0.0 6 6 96 3 0.0 6 2 1 97 4 0.0 6 4 98 6 0.0 6 6 99 4 0.0 6 4 100 17 0.0 6 16 1 101 16 0.0 6 14 2 102 10 0.0 6 9 1 103 8 0.0 6 8 104 10 0.0 6 9 0 0 0 0 0 1 105 7 0.0 6 7 106 28 0.0 6 26 2 107 9 0.0 6 8 0 0 1 108 16 0.0 6 16 109 23 0.0 6 23 110 9 0.0 6 9 111 16 0.0 6 16 112 28 0.0 6 27 1 113 14 0.0 6 14 114 8 0.0 6 6 2 115 10 0.0 6 9 1 116 12 0.0 6 11 0 0 0 0 1 117 7 0.0 6 7 118 7 0.0 6 6 1 119 16 0.0 6 15 1 120 9 0.0 6 9 121 23 0.0 6 22 1 122 13 0.0 6 10 3 123 16 0.0 6 16 124 22 0.0 6 19 1 1 1 125 23 0.0 6 20 2 1 126 26 0.0 6 24 2 127 26 0.0 6 25 1 128 33 0.0 6 32 1 129 24 0.0 6 24 130 32 0.0 6 30 2 131 24 0.0 6 24 132 23 0.0 6 22 1 133 16 0.0 6 15 1 134 23 0.0 6 22 1 135 38 0.0 6 36 2 136 13 0.0 6 12 1 137 19 0.0 6 16 3 138 36 0.0 6 35 0 0 1 139 30 0.0 6 28 2 140 28 0.0 6 27 1 141 32 0.0 6 31 1 142 38 0.0 6 35 3 143 133 0.0 6 132 1 144 160 0.0 6 160 145 231 0.0 6 228 2 1 146 131 0.0 6 131 147 133 0.0 6 132 1 148 137 0.0 6 132 5 149 219 0.0 6 218 1 150 112 0.0 6 112 151 101 0.0 6 97 4 152 116 0.0 6 112 4 153 123 0.0 6 119 4 154 73 0.0 6 69 3 1 155 89 0.0 6 89 156 87 0.0 6 85 2 157 81 0.0 6 81 158 85 0.0 6 85 159 98 0.0 6 98 160 112 0.0 6 109 3 161 119 0.0 6 116 3 162 89 0.0 6 88 1 163 109 0.0 6 108 1 164 81 0.0 6 79 2 165 100 0.0 6 100 166 80 0.0 6 77 3 167 102 0.0 6 101 1 168 63 0.0 6 63 169 119 0.0 6 115 4 170 101 0.0 6 101 171 86 0.0 6 86 172 97 0.0 6 95 2 173 114 0.0 6 106 6 1 1 174 85 0.0 6 84 1 175 99 0.0 6 97 2 176 112 0.0 6 112 177 156 0.0 6 152 3 1 178 121 0.0 6 118 3 179 91 0.0 6 90 1 180 98 0.0 6 98 181 189 0.0 6 185 4 182 128 0.0 6 124 4 183 81 0.0 6 77 4 184 116 0.0 6 113 3 185 120 0.0 6 118 2 186 130 0.0 6 127 2 0 0 0 1 187 103 0.0 6 100 2 1 188 177 0.0 6 174 3 189 187 0.0 6 181 6 190 168 0.0 6 163 5 191 185 0.0 6 182 3 192 189 0.0 6 186 3 193 240 0.0 6 238 2 194 221 0.0 6 216 3 1 1 195 314 0.0 6 305 9 196 297 0.0 6 290 6 1 197 370 0.0 6 361 9 198 333 0.0 6 327 6 199 334 0.0 6 330 4 200 281 0.0 6 271 9 1 201 247 0.0 6 241 6 202 227 0.0 6 222 5 203 284 0.0 6 276 8 204 267 0.0 6 261 4 1 1 205 332 0.0 6 326 5 0 0 1 206 329 0.0 6 321 8 207 393 0.0 6 385 8 208 356 0.0 6 349 7 209 445 0.0 6 432 13 210 377 0.0 6 365 12 211 584 0.0 6 568 15 1 212 1106 0.0 6 1084 22 213 778 0.0 6 765 13 214 599 0.0 6 584 15 215 875 0.0 6 855 20 216 992 0.0 6 974 16 2 217 622 0.0 6 610 12 218 490 0.0 6 478 12 219 409 0.0 6 400 9 220 306 0.0 6 294 12 221 297 0.0 6 287 10 222 371 0.0 6 359 12 223 441 0.0 6 434 7 224 382 0.0 6 377 5 225 418 0.0 6 411 6 1 226 666 0.0 6 655 11 227 610 0.0 6 594 14 1 1 228 576 0.0 6 565 11 229 534 0.0 6 518 15 1 230 354 0.0 6 344 10 231 381 0.0 6 374 7 232 361 0.0 6 358 3 233 576 0.0 6 564 12 234 473 0.0 6 466 7 235 368 0.0 6 357 11 236 440 0.0 6 428 12 237 736 0.0 6 722 14 238 457 0.0 6 440 16 1 239 475 0.0 6 457 18 240 565 0.0 6 555 10 241 477 0.0 6 466 10 1 242 842 0.0 6 821 20 1 243 563 0.0 6 553 10 244 693 0.0 6 685 7 1 245 600 0.0 6 585 14 0 0 0 1 246 509 0.0 6 491 18 247 393 0.0 6 383 9 0 0 1 248 452 0.0 6 443 8 0 0 1 249 369 0.0 6 356 10 2 1 250 784 0.0 6 774 10 251 920 0.0 6 899 21 252 738 0.0 6 725 13 253 551 0.0 6 533 18 254 1346 0.0 6 1315 29 2 255 880 0.0 6 851 27 2 256 2296 0.0 6 2259 36 1 257 921 0.0 6 899 22 258 709 0.0 6 691 16 1 1 259 1341 0.0 6 1306 35 260 1900 0.0 6 1858 41 0 1 261 2229 0.0 6 2179 50 262 1463 0.0 6 1423 39 1 263 2077 0.0 6 2044 33 264 1079 0.0 6 1049 29 1 265 1847 0.0 6 1799 47 0 0 0 1 266 1172 0.0 6 1134 38 267 2713 0.0 6 2664 49 268 1127 0.0 6 1097 30 269 916 0.0 6 884 31 1 270 1452 0.0 6 1421 30 1 271 1852 0.0 6 1806 44 0 2 272 1076 0.0 6 1041 35 273 2326 0.0 6 2270 51 4 1 274 3018 0.0 6 2961 54 3 275 2516 0.0 6 2470 45 0 1 276 2120 0.0 6 2067 51 1 0 1 277 2819 0.0 6 2750 65 2 1 0 1 278 4164 0.0 6 4056 104 3 1 279 21678 0.0 6 21089 566 18 3 1 1 280 25738 0.0 6 24956 747 33 2 281 163 0.0 6 143 19 1 282 33 0.0 6 22 5 4 1 0 1 283 12 0.0 6 9 1 1 1 284 4 0.0 6 4 287 2 0.0 6 2 288 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 211 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 53 1 0.0 6 1 62 1 0.0 6 1 71 3 0.0 6 3 72 2 0.0 6 2 73 9 0.0 6 9 74 2 0.0 6 2 75 5 0.0 6 5 76 5 0.0 6 5 77 5 0.0 6 5 78 1 0.0 6 1 79 2 0.0 6 2 80 3 0.0 6 3 81 1 0.0 6 1 82 1 0.0 6 1 83 1 0.0 6 1 84 1 0.0 6 1 85 1 0.0 6 1 88 3 0.0 6 3 89 1 0.0 6 1 90 1 0.0 6 1 91 1 0.0 6 1 93 2 0.0 6 1 1 95 1 0.0 6 0 0 0 1 96 1 0.0 6 1 97 1 0.0 6 1 98 1 0.0 6 1 99 1 0.0 6 1 101 1 0.0 6 1 102 2 0.0 6 2 103 1 0.0 6 1 104 3 0.0 6 3 105 1 0.0 6 1 106 2 0.0 6 2 107 2 0.0 6 2 108 3 0.0 6 3 109 2 0.0 6 2 110 2 0.0 6 2 111 1 0.0 6 1 113 3 0.0 6 3 115 3 0.0 6 3 116 2 0.0 6 2 117 1 0.0 6 1 119 1 0.0 6 1 120 1 0.0 6 1 121 2 0.0 6 2 122 1 0.0 6 1 123 1 0.0 6 1 124 1 0.0 6 1 125 2 0.0 6 2 126 4 0.0 6 4 127 1 0.0 6 1 129 2 0.0 6 2 130 1 0.0 6 1 131 1 0.0 6 1 132 1 0.0 6 1 133 1 0.0 6 1 134 2 0.0 6 2 135 1 0.0 6 1 136 2 0.0 6 2 137 1 0.0 6 1 139 1 0.0 6 1 140 1 0.0 6 1 141 1 0.0 6 1 143 1 0.0 6 1 145 1 0.0 6 1 146 2 0.0 6 2 148 3 0.0 6 3 149 1 0.0 6 1 150 1 0.0 6 1 153 2 0.0 6 2 154 1 0.0 6 1 155 2 0.0 6 2 156 1 0.0 6 1 157 3 0.0 6 3 158 1 0.0 6 1 159 2 0.0 6 2 161 2 0.0 6 2 162 1 0.0 6 1 163 6 0.0 6 6 165 1 0.0 6 1 166 2 0.0 6 2 167 1 0.0 6 1 168 2 0.0 6 2 169 1 0.0 6 1 170 1 0.0 6 1 171 1 0.0 6 1 172 1 0.0 6 1 173 1 0.0 6 1 175 1 0.0 6 1 176 3 0.0 6 3 179 1 0.0 6 1 180 2 0.0 6 2 183 1 0.0 6 1 184 1 0.0 6 1 188 6 0.0 6 6 189 1 0.0 6 1 191 1 0.0 6 1 192 3 0.0 6 3 193 1 0.0 6 1 194 1 0.0 6 1 195 2 0.0 6 2 196 1 0.0 6 1 197 1 0.0 6 1 199 2 0.0 6 2 201 1 0.0 6 1 202 1 0.0 6 1 204 1 0.0 6 1 206 1 0.0 6 1 208 1 0.0 6 1 214 2 0.0 6 2 215 1 0.0 6 1 216 1 0.0 6 1 221 1 0.0 6 1 222 1 0.0 6 1 229 1 0.0 6 1 235 1 0.0 6 1 237 1 0.0 6 1 240 1 0.0 6 1 244 1 0.0 6 1 251 2 0.0 6 2 258 1 0.0 6 1 260 1 0.0 6 1 262 1 0.0 6 1 267 1 0.0 6 1 268 1 0.0 6 1 270 1 0.0 6 1 280 1 0.0 6 1 281 1 0.0 6 1 287 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 130139 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 2038.2 0 1 1 9 3 0.5 2 3 10 9 0.1 3 9 11 9 0.0 3 8 1 12 1 0.0 3 1 13 12 0.0 3 10 2 14 12 0.0 4 12 15 2 0.0 4 1 0 0 0 1 16 9 0.0 4 7 1 1 17 28 0.0 5 23 4 0 1 18 15 0.0 5 11 4 19 14 0.0 5 11 3 20 89 0.0 6 87 2 21 69 0.0 6 68 1 22 43 0.0 6 40 3 23 40 0.0 6 39 1 24 30 0.0 6 28 2 25 41 0.0 6 35 6 26 24 0.0 6 23 1 27 31 0.0 6 28 3 28 14 0.0 6 12 2 29 31 0.0 6 28 3 30 22 0.0 6 21 1 31 22 0.0 6 20 2 32 21 0.0 6 20 0 0 1 33 12 0.0 6 10 2 34 21 0.0 6 19 2 35 12 0.0 6 11 1 36 12 0.0 6 9 1 1 0 0 1 37 16 0.0 6 13 2 0 1 38 8 0.0 6 8 39 6 0.0 6 6 40 12 0.0 6 11 0 1 41 15 0.0 6 12 2 0 0 1 42 17 0.0 6 15 2 43 20 0.0 6 16 4 44 28 0.0 6 26 2 45 17 0.0 6 15 1 0 0 1 46 26 0.0 6 22 3 0 0 1 47 40 0.0 6 37 2 1 48 13 0.0 6 11 2 49 25 0.0 6 24 1 50 37 0.0 6 32 2 3 51 21 0.0 6 19 1 1 52 41 0.0 6 35 5 0 0 1 53 42 0.0 6 36 6 54 36 0.0 6 33 3 55 27 0.0 6 23 4 56 36 0.0 6 30 5 1 57 37 0.0 6 32 5 58 33 0.0 6 28 5 59 23 0.0 6 23 60 33 0.0 6 32 1 61 24 0.0 6 19 3 1 0 1 62 23 0.0 6 21 2 63 25 0.0 6 22 3 64 25 0.0 6 25 65 17 0.0 6 14 2 0 0 0 1 66 53 0.0 6 45 6 1 1 67 239 0.0 6 224 12 2 0 1 68 45 0.0 6 42 2 1 69 98 0.0 6 91 6 1 70 27 0.0 6 23 4 71 77 0.0 6 70 6 1 72 75 0.0 6 68 6 1 73 20 0.0 6 18 2 74 17 0.0 6 14 2 0 1 75 33 0.0 6 32 0 0 0 1 76 63 0.0 6 60 3 77 32 0.0 6 30 2 78 109 0.0 6 100 9 79 52 0.0 6 48 2 1 0 1 80 30 0.0 6 26 4 81 40 0.0 6 36 4 82 85 0.0 6 80 5 83 96 0.0 6 91 3 2 84 66 0.0 6 62 2 1 0 1 85 28 0.0 6 24 3 1 86 25 0.0 6 21 4 87 48 0.0 6 43 5 88 27 0.0 6 22 4 1 89 54 0.0 6 49 5 90 44 0.0 6 41 2 0 0 0 1 91 62 0.0 6 60 2 92 63 0.0 6 57 3 2 1 93 84 0.0 6 75 8 0 0 1 94 105 0.0 6 98 7 95 66 0.0 6 63 2 1 96 83 0.0 6 75 8 97 169 0.0 6 160 9 98 83 0.0 6 73 9 1 99 122 0.0 6 116 5 1 100 125 0.0 6 116 9 101 109 0.0 6 102 6 1 102 113 0.0 6 109 4 103 127 0.0 6 119 7 1 104 191 0.0 6 171 20 105 156 0.0 6 150 5 1 106 203 0.0 6 182 16 2 1 1 1 107 92 0.0 6 84 8 108 147 0.0 6 139 7 1 109 82 0.0 6 77 4 0 0 1 110 72 0.0 6 65 6 1 111 76 0.0 6 71 5 112 84 0.0 6 80 4 113 77 0.0 6 74 2 0 0 0 0 1 114 98 0.0 6 97 1 115 113 0.0 6 107 5 1 116 120 0.0 6 112 6 2 117 92 0.0 6 89 3 118 135 0.0 6 127 8 119 109 0.0 6 102 6 1 120 126 0.0 6 117 7 2 121 117 0.0 6 109 8 122 145 0.0 6 138 6 1 123 108 0.0 6 106 2 124 130 0.0 6 125 3 1 1 125 196 0.0 6 185 8 2 0 1 126 206 0.0 6 198 7 1 127 183 0.0 6 176 6 1 128 161 0.0 6 154 6 0 0 1 129 146 0.0 6 139 5 0 0 1 1 130 208 0.0 6 202 6 131 193 0.0 6 188 5 132 198 0.0 6 192 6 133 186 0.0 6 180 6 134 271 0.0 6 261 10 135 284 0.0 6 278 6 136 173 0.0 6 168 4 0 0 0 1 137 352 0.0 6 344 8 138 192 0.0 6 186 5 0 0 1 139 379 0.0 6 369 9 0 0 0 1 140 484 0.0 6 469 13 1 0 1 141 514 0.0 6 497 16 0 0 1 142 557 0.0 6 537 16 3 0 0 0 1 143 577 0.0 6 562 12 1 0 1 1 144 541 0.0 6 525 15 0 1 145 659 0.0 6 636 21 1 0 1 146 554 0.0 6 540 13 0 0 1 147 443 0.0 6 430 12 1 148 746 0.0 6 718 25 2 0 1 149 792 0.0 6 764 25 1 0 2 150 961 0.0 6 946 12 3 151 490 0.0 6 473 15 2 152 967 0.0 6 929 37 1 153 1038 0.0 6 1012 25 0 1 154 1646 0.0 6 1600 44 1 0 1 155 1036 0.0 6 1008 26 1 1 156 735 0.0 6 714 21 157 1223 0.0 6 1195 25 2 1 158 852 0.0 6 830 21 1 159 1093 0.0 6 1062 28 1 1 1 160 754 0.0 6 730 21 1 1 1 161 633 0.0 6 607 24 1 0 1 162 1166 0.0 6 1139 24 2 0 1 163 638 0.0 6 614 21 2 1 164 916 0.0 6 878 36 1 0 1 165 628 0.0 6 610 16 1 0 0 1 166 779 0.0 6 750 26 0 0 2 0 1 167 581 0.0 6 564 17 168 1010 0.0 6 977 32 0 0 1 169 709 0.0 6 691 18 170 972 0.0 6 938 32 2 171 909 0.0 6 880 28 1 172 1172 0.0 6 1139 31 2 173 712 0.0 6 687 21 1 2 1 174 1011 0.0 6 975 35 0 0 1 175 1590 0.0 6 1548 39 2 0 1 176 1163 0.0 6 1126 35 1 0 1 177 893 0.0 6 861 30 1 0 1 178 1143 0.0 6 1107 35 0 0 1 179 1367 0.0 6 1319 41 7 180 921 0.0 6 893 26 0 1 1 181 1673 0.0 6 1633 36 3 0 1 182 984 0.0 6 948 32 2 0 2 183 1830 0.0 6 1789 39 0 0 1 0 1 184 1306 0.0 6 1272 31 0 1 2 185 1375 0.0 6 1336 37 1 1 186 1529 0.0 6 1479 43 6 1 187 1483 0.0 6 1441 41 0 1 188 1910 0.0 6 1844 62 3 0 1 189 1550 0.0 6 1478 70 0 1 1 190 2721 0.0 6 2646 71 3 0 1 191 2830 0.0 6 2750 76 1 0 2 1 192 1630 0.0 6 1573 53 0 2 1 1 193 1496 0.0 6 1435 54 1 2 2 2 194 2227 0.0 6 2162 61 2 1 1 195 1902 0.0 6 1824 75 2 1 196 3275 0.0 6 3176 95 3 1 197 1618 0.0 6 1573 42 3 198 2122 0.0 6 2049 69 3 0 1 199 2768 0.0 6 2700 63 2 0 2 0 1 200 2340 0.0 6 2265 68 4 1 2 201 2344 0.0 6 2286 55 2 0 1 202 2386 0.0 6 2310 73 2 0 1 203 1510 0.0 6 1469 39 2 204 2358 0.0 6 2286 68 2 0 2 205 1256 0.0 6 1215 39 0 1 1 206 1161 0.0 6 1133 26 1 0 1 207 920 0.0 6 870 50 208 2757 0.0 6 2679 76 1 1 209 805 0.0 6 773 28 3 1 210 1000 0.0 6 974 25 1 211 849 0.0 6 827 19 1 2 212 841 0.0 6 816 25 213 615 0.0 6 593 20 2 214 1587 0.0 6 1558 29 215 1281 0.0 6 1248 28 1 0 3 1 216 1199 0.0 6 1164 31 1 2 1 217 1304 0.0 6 1277 26 1 218 554 0.0 6 532 20 2 219 782 0.0 6 753 24 3 0 2 220 1624 0.0 6 1570 50 2 0 2 221 976 0.0 6 942 32 1 0 0 1 222 690 0.0 6 668 20 1 0 1 223 1097 0.0 6 1063 30 1 1 2 224 508 0.0 6 501 6 0 1 225 455 0.0 6 439 16 226 469 0.0 6 446 18 5 227 735 0.0 6 710 21 2 2 228 1722 0.0 6 1675 46 0 0 1 229 886 0.0 6 855 27 3 1 230 823 0.0 6 786 35 2 231 4941 0.0 6 4806 130 3 1 1 232 712 0.0 6 683 26 3 233 594 0.0 6 573 19 1 0 1 234 274 0.0 6 263 10 1 235 242 0.0 6 232 10 236 125 0.0 6 122 2 0 0 0 1 237 105 0.0 6 99 5 1 238 474 0.0 6 454 19 0 0 1 239 605 0.0 6 587 17 0 0 1 240 90 0.0 6 88 2 241 229 0.0 6 218 10 1 242 253 0.0 6 247 6 243 172 0.0 6 169 3 244 112 0.0 6 111 1 245 179 0.0 6 177 2 246 291 0.0 6 282 9 247 143 0.0 6 142 1 248 381 0.0 6 368 12 0 0 1 249 52 0.0 6 51 1 250 60 0.0 6 60 251 84 0.0 6 79 4 0 1 252 53 0.0 6 49 3 1 253 116 0.0 6 113 3 254 5 0.0 6 5 255 7 0.0 6 6 1 256 1 0.0 6 1 259 1 0.0 6 1 260 2 0.0 6 2 262 1 0.0 6 1 263 1 0.0 6 1 270 1 0.0 6 1 274 1 0.0 6 1 276 1 0.0 6 1 277 2 0.0 6 2 278 105 0.0 6 102 3 279 117 0.0 6 111 5 0 0 0 1 280 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 210 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 58 1 0.0 6 1 71 1 0.0 6 1 72 4 0.0 6 4 73 6 0.0 6 6 74 6 0.0 6 6 75 2 0.0 6 2 76 5 0.0 6 5 77 6 0.0 6 6 78 6 0.0 6 6 79 3 0.0 6 3 80 4 0.0 6 4 81 3 0.0 6 3 82 3 0.0 6 3 85 1 0.0 6 1 87 1 0.0 6 1 89 1 0.0 6 1 90 2 0.0 6 2 91 1 0.0 6 1 92 3 0.0 6 3 93 1 0.0 6 1 94 3 0.0 6 3 95 2 0.0 6 2 96 2 0.0 6 2 97 2 0.0 6 2 98 1 0.0 6 1 100 1 0.0 6 1 101 2 0.0 6 2 103 2 0.0 6 2 105 4 0.0 6 4 106 2 0.0 6 2 107 2 0.0 6 2 108 1 0.0 6 1 109 2 0.0 6 2 110 2 0.0 6 2 111 2 0.0 6 1 1 112 1 0.0 6 1 113 4 0.0 6 4 116 1 0.0 6 1 117 1 0.0 6 1 119 1 0.0 6 1 120 1 0.0 6 1 121 2 0.0 6 2 122 1 0.0 6 1 123 2 0.0 6 2 124 1 0.0 6 1 125 1 0.0 6 0 0 0 0 0 1 127 1 0.0 6 1 128 1 0.0 6 1 129 1 0.0 6 1 130 1 0.0 6 1 131 1 0.0 6 1 133 1 0.0 6 1 134 4 0.0 6 4 135 1 0.0 6 1 136 3 0.0 6 3 137 1 0.0 6 1 138 2 0.0 6 2 140 1 0.0 6 1 141 3 0.0 6 3 142 1 0.0 6 1 143 1 0.0 6 1 144 1 0.0 6 1 146 3 0.0 6 3 147 4 0.0 6 4 148 1 0.0 6 1 149 1 0.0 6 1 151 2 0.0 6 2 152 1 0.0 6 1 153 2 0.0 6 2 155 1 0.0 6 1 156 1 0.0 6 1 158 1 0.0 6 1 159 2 0.0 6 2 160 1 0.0 6 1 162 1 0.0 6 1 163 1 0.0 6 1 164 8 0.0 6 8 165 1 0.0 6 1 167 7 0.0 6 7 168 2 0.0 6 2 169 4 0.0 6 4 170 2 0.0 6 2 172 2 0.0 6 2 173 2 0.0 6 2 174 2 0.0 6 2 176 1 0.0 6 1 179 2 0.0 6 2 180 1 0.0 6 1 181 3 0.0 6 3 182 2 0.0 6 2 183 1 0.0 6 1 185 1 0.0 6 1 186 1 0.0 6 1 187 1 0.0 6 1 188 1 0.0 6 1 189 2 0.0 6 2 190 1 0.0 6 1 192 2 0.0 6 2 195 1 0.0 6 1 196 1 0.0 6 1 201 1 0.0 6 1 207 1 0.0 6 1 217 1 0.0 6 1 219 1 0.0 6 1 226 1 0.0 6 1 230 1 0.0 6 1 239 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_04_3_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_04_230_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_04_3_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_04_230_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.72 s (81 µs/read; 0.74 M reads/minute). === Summary === Total read pairs processed: 21,209 Read 1 with adapter: 21,208 (100.0%) Read 2 with adapter: 21,202 (100.0%) == Read fate breakdown == Pairs that were too short: 2 (0.0%) Pairs discarded as untrimmed: 7 (0.0%) Pairs written (passing filters): 21,200 (100.0%) Total basepairs processed: 10,219,000 bp Read 1: 5,739,076 bp Read 2: 4,479,924 bp Total written (filtered): 1,290,968 bp (12.6%) Read 1: 671,411 bp Read 2: 619,557 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 20918 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 331.4 0 1 6 1 5.2 1 0 0 1 7 1 1.3 2 1 9 2 0.1 2 1 1 15 2 0.0 4 2 16 2 0.0 4 1 1 17 1 0.0 5 1 20 8 0.0 6 8 21 10 0.0 6 10 22 6 0.0 6 5 1 23 4 0.0 6 4 24 1 0.0 6 1 25 2 0.0 6 2 26 2 0.0 6 2 28 4 0.0 6 3 1 30 1 0.0 6 1 31 2 0.0 6 1 1 34 2 0.0 6 1 1 35 1 0.0 6 1 36 1 0.0 6 1 39 1 0.0 6 1 40 3 0.0 6 3 43 2 0.0 6 2 45 2 0.0 6 2 50 1 0.0 6 1 51 1 0.0 6 1 52 2 0.0 6 2 53 1 0.0 6 1 55 1 0.0 6 1 57 1 0.0 6 1 58 2 0.0 6 2 59 2 0.0 6 2 61 2 0.0 6 2 62 1 0.0 6 1 64 1 0.0 6 1 65 3 0.0 6 3 66 1 0.0 6 0 1 68 3 0.0 6 3 69 1 0.0 6 1 71 2 0.0 6 2 73 2 0.0 6 2 74 3 0.0 6 3 75 1 0.0 6 1 76 1 0.0 6 1 77 1 0.0 6 1 78 1 0.0 6 1 79 4 0.0 6 3 1 80 1 0.0 6 1 81 1 0.0 6 1 82 1 0.0 6 1 84 4 0.0 6 3 1 85 3 0.0 6 3 86 2 0.0 6 2 87 3 0.0 6 2 1 88 2 0.0 6 2 89 1 0.0 6 1 90 2 0.0 6 2 91 2 0.0 6 2 93 1 0.0 6 1 94 2 0.0 6 2 99 1 0.0 6 1 100 3 0.0 6 2 1 101 3 0.0 6 2 1 102 5 0.0 6 5 103 1 0.0 6 1 104 1 0.0 6 1 106 2 0.0 6 2 107 5 0.0 6 5 108 3 0.0 6 3 109 3 0.0 6 3 110 1 0.0 6 1 111 1 0.0 6 1 112 2 0.0 6 2 113 1 0.0 6 1 114 2 0.0 6 2 115 2 0.0 6 2 117 2 0.0 6 2 118 3 0.0 6 2 1 119 1 0.0 6 1 120 1 0.0 6 1 121 3 0.0 6 3 122 4 0.0 6 3 1 123 4 0.0 6 3 1 124 1 0.0 6 1 125 5 0.0 6 5 126 4 0.0 6 3 1 127 2 0.0 6 2 128 7 0.0 6 7 129 5 0.0 6 5 130 9 0.0 6 9 131 3 0.0 6 3 132 6 0.0 6 5 1 133 8 0.0 6 8 134 9 0.0 6 8 1 135 4 0.0 6 4 136 10 0.0 6 9 1 137 7 0.0 6 7 138 4 0.0 6 4 139 9 0.0 6 8 1 140 3 0.0 6 3 141 5 0.0 6 4 1 142 8 0.0 6 7 1 143 5 0.0 6 5 144 12 0.0 6 12 145 20 0.0 6 19 1 146 7 0.0 6 7 147 9 0.0 6 8 1 148 8 0.0 6 8 149 4 0.0 6 4 150 9 0.0 6 9 151 16 0.0 6 16 152 12 0.0 6 11 1 153 12 0.0 6 12 154 1 0.0 6 1 155 7 0.0 6 6 1 156 18 0.0 6 16 2 157 13 0.0 6 13 158 13 0.0 6 13 159 12 0.0 6 12 160 23 0.0 6 21 2 161 16 0.0 6 16 162 31 0.0 6 30 1 163 166 0.0 6 165 1 164 48 0.0 6 47 1 165 18 0.0 6 18 166 16 0.0 6 16 167 13 0.0 6 13 168 11 0.0 6 10 1 169 24 0.0 6 24 170 30 0.0 6 30 171 44 0.0 6 44 172 19 0.0 6 17 2 173 38 0.0 6 36 2 174 46 0.0 6 46 175 42 0.0 6 41 1 176 22 0.0 6 21 1 177 48 0.0 6 48 178 54 0.0 6 53 1 179 33 0.0 6 33 180 33 0.0 6 32 1 181 18 0.0 6 18 182 28 0.0 6 26 2 183 44 0.0 6 43 1 184 21 0.0 6 17 4 185 41 0.0 6 40 1 186 61 0.0 6 58 3 187 80 0.0 6 79 1 188 63 0.0 6 62 1 189 62 0.0 6 61 1 190 301 0.0 6 301 191 79 0.0 6 78 1 192 35 0.0 6 35 193 38 0.0 6 38 194 50 0.0 6 50 195 97 0.0 6 96 1 196 130 0.0 6 128 2 197 106 0.0 6 105 1 198 97 0.0 6 95 2 199 107 0.0 6 106 1 200 115 0.0 6 114 0 1 201 105 0.0 6 103 2 202 119 0.0 6 118 1 203 118 0.0 6 114 4 204 120 0.0 6 119 1 205 163 0.0 6 163 206 177 0.0 6 176 1 207 607 0.0 6 603 4 208 265 0.0 6 261 4 209 408 0.0 6 404 4 210 111 0.0 6 110 1 211 164 0.0 6 161 3 212 122 0.0 6 119 2 0 0 1 213 123 0.0 6 122 1 214 216 0.0 6 212 3 1 215 204 0.0 6 202 2 216 463 0.0 6 461 2 217 91 0.0 6 90 1 218 101 0.0 6 100 1 219 107 0.0 6 106 0 0 0 0 0 1 220 194 0.0 6 193 1 221 100 0.0 6 98 2 222 80 0.0 6 79 1 223 105 0.0 6 103 2 224 125 0.0 6 123 2 225 82 0.0 6 80 2 226 182 0.0 6 180 2 227 131 0.0 6 129 2 228 121 0.0 6 121 229 117 0.0 6 116 1 230 147 0.0 6 144 3 231 117 0.0 6 116 1 232 147 0.0 6 146 1 233 91 0.0 6 90 1 234 110 0.0 6 107 3 235 81 0.0 6 81 236 104 0.0 6 103 1 237 173 0.0 6 169 4 238 133 0.0 6 131 2 239 141 0.0 6 138 3 240 210 0.0 6 206 4 241 185 0.0 6 180 4 1 242 351 0.0 6 347 4 243 92 0.0 6 90 2 244 91 0.0 6 88 3 245 100 0.0 6 96 4 246 76 0.0 6 74 2 247 158 0.0 6 151 7 248 118 0.0 6 112 6 249 202 0.0 6 198 4 250 153 0.0 6 145 8 251 104 0.0 6 102 2 252 310 0.0 6 297 13 253 130 0.0 6 124 5 0 0 1 254 128 0.0 6 125 3 255 145 0.0 6 138 7 256 221 0.0 6 215 6 257 178 0.0 6 175 3 258 247 0.0 6 240 7 259 342 0.0 6 333 8 0 1 260 220 0.0 6 218 2 261 327 0.0 6 315 12 262 121 0.0 6 114 5 1 1 263 258 0.0 6 246 12 264 126 0.0 6 121 5 265 86 0.0 6 81 5 266 170 0.0 6 167 3 267 158 0.0 6 152 6 268 102 0.0 6 98 4 269 193 0.0 6 189 4 270 223 0.0 6 220 3 271 77 0.0 6 76 1 272 90 0.0 6 85 5 273 114 0.0 6 108 5 1 274 129 0.0 6 123 6 275 176 0.0 6 171 5 276 427 0.0 6 411 15 1 277 354 0.0 6 342 12 278 475 0.0 6 457 18 279 3137 0.0 6 3036 101 280 1307 0.0 6 1252 54 1 281 22 0.0 6 17 4 1 282 2 0.0 6 2 283 2 0.0 6 2 285 1 0.0 6 1 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 290 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 52 1 0.0 6 1 75 1 0.0 6 1 90 1 0.0 6 1 93 4 0.0 6 4 94 5 0.0 6 5 106 1 0.0 6 1 108 1 0.0 6 1 109 3 0.0 6 3 120 4 0.0 6 4 121 14 0.0 6 14 122 1 0.0 6 1 126 1 0.0 6 1 127 5 0.0 6 5 133 1 0.0 6 1 134 1 0.0 6 1 135 2 0.0 6 2 136 3 0.0 6 3 137 9 0.0 6 9 138 34 0.0 6 34 139 1 0.0 6 1 143 1 0.0 6 1 146 4 0.0 6 4 147 12 0.0 6 12 150 2 0.0 6 2 151 10 0.0 6 10 154 1 0.0 6 1 168 1 0.0 6 1 169 3 0.0 6 3 170 2 0.0 6 2 171 2 0.0 6 2 172 17 0.0 6 17 173 67 0.0 6 67 178 1 0.0 6 1 179 11 0.0 6 11 180 28 0.0 6 28 181 1 0.0 6 1 196 8 0.0 6 8 197 1 0.0 6 1 209 4 0.0 6 4 223 1 0.0 6 1 235 1 0.0 6 1 238 3 0.0 6 3 239 1 0.0 6 1 241 1 0.0 6 1 242 1 0.0 6 1 245 3 0.0 6 3 246 7 0.0 6 7 247 1 0.0 6 1 252 1 0.0 6 1 299 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 20962 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.8% G: 0.1% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 12 1 0.0 3 1 14 4 0.0 4 4 16 1 0.0 4 1 17 4 0.0 5 4 18 3 0.0 5 1 2 19 15 0.0 5 14 1 20 9 0.0 6 9 21 8 0.0 6 7 1 22 6 0.0 6 6 23 7 0.0 6 6 1 24 4 0.0 6 4 25 3 0.0 6 3 26 3 0.0 6 3 27 2 0.0 6 2 28 4 0.0 6 4 29 5 0.0 6 3 1 0 0 0 0 1 30 3 0.0 6 3 31 1 0.0 6 1 32 3 0.0 6 3 33 3 0.0 6 3 34 3 0.0 6 3 35 1 0.0 6 1 36 1 0.0 6 1 37 1 0.0 6 1 38 3 0.0 6 2 1 39 3 0.0 6 3 40 2 0.0 6 1 1 41 3 0.0 6 2 0 1 42 2 0.0 6 2 43 4 0.0 6 3 0 0 1 44 3 0.0 6 3 45 4 0.0 6 4 46 2 0.0 6 2 47 5 0.0 6 4 1 48 5 0.0 6 5 49 3 0.0 6 3 50 2 0.0 6 2 51 5 0.0 6 2 3 52 7 0.0 6 6 0 0 0 1 53 1 0.0 6 1 54 5 0.0 6 5 55 5 0.0 6 3 2 56 13 0.0 6 12 1 57 3 0.0 6 3 58 4 0.0 6 4 59 7 0.0 6 6 0 1 60 2 0.0 6 2 61 4 0.0 6 4 62 4 0.0 6 4 63 6 0.0 6 6 64 3 0.0 6 3 65 3 0.0 6 3 66 4 0.0 6 4 67 2 0.0 6 2 68 9 0.0 6 6 3 69 9 0.0 6 9 70 38 0.0 6 35 3 71 12 0.0 6 11 1 72 21 0.0 6 17 4 73 6 0.0 6 6 74 9 0.0 6 9 75 12 0.0 6 12 76 10 0.0 6 9 1 77 11 0.0 6 10 0 0 0 1 78 8 0.0 6 7 1 79 8 0.0 6 8 80 4 0.0 6 4 81 7 0.0 6 7 82 7 0.0 6 7 83 5 0.0 6 5 84 13 0.0 6 10 3 85 23 0.0 6 18 5 86 15 0.0 6 15 87 10 0.0 6 9 1 88 10 0.0 6 9 1 89 10 0.0 6 9 1 90 7 0.0 6 7 91 4 0.0 6 4 92 7 0.0 6 5 1 1 93 15 0.0 6 13 1 1 94 7 0.0 6 7 95 13 0.0 6 13 96 9 0.0 6 8 1 97 12 0.0 6 9 3 98 9 0.0 6 9 99 14 0.0 6 14 100 21 0.0 6 21 101 13 0.0 6 13 102 20 0.0 6 19 1 103 31 0.0 6 26 5 104 17 0.0 6 16 1 105 17 0.0 6 16 1 106 12 0.0 6 9 3 107 19 0.0 6 18 1 108 22 0.0 6 21 0 1 109 11 0.0 6 11 110 18 0.0 6 15 2 0 0 0 0 1 111 12 0.0 6 11 1 112 18 0.0 6 16 2 113 13 0.0 6 12 0 0 0 1 114 15 0.0 6 15 115 20 0.0 6 19 1 116 26 0.0 6 24 2 117 19 0.0 6 18 1 118 26 0.0 6 25 0 0 0 0 1 119 31 0.0 6 30 1 120 41 0.0 6 40 1 121 25 0.0 6 24 1 122 22 0.0 6 22 123 28 0.0 6 27 1 124 24 0.0 6 23 1 125 28 0.0 6 27 1 126 22 0.0 6 22 127 30 0.0 6 27 3 128 51 0.0 6 50 1 129 37 0.0 6 37 130 47 0.0 6 45 2 131 51 0.0 6 50 1 132 38 0.0 6 37 1 133 66 0.0 6 63 3 134 55 0.0 6 54 1 135 90 0.0 6 87 3 136 68 0.0 6 67 1 137 98 0.0 6 91 7 138 73 0.0 6 68 5 139 75 0.0 6 71 3 0 0 1 140 100 0.0 6 94 6 141 95 0.0 6 94 1 142 120 0.0 6 113 5 2 143 105 0.0 6 101 3 0 0 1 144 139 0.0 6 136 3 145 116 0.0 6 113 2 1 146 127 0.0 6 123 2 1 0 0 0 1 147 108 0.0 6 104 4 148 117 0.0 6 114 3 149 186 0.0 6 179 7 150 130 0.0 6 126 4 151 159 0.0 6 156 2 1 152 225 0.0 6 218 6 0 0 0 0 1 153 189 0.0 6 184 5 154 147 0.0 6 145 2 155 237 0.0 6 232 5 156 166 0.0 6 163 2 0 0 1 157 167 0.0 6 162 5 158 212 0.0 6 204 8 159 176 0.0 6 172 4 160 188 0.0 6 182 6 161 184 0.0 6 179 4 1 162 260 0.0 6 258 2 163 176 0.0 6 173 2 0 0 0 1 164 187 0.0 6 182 5 165 238 0.0 6 236 2 166 234 0.0 6 230 4 167 178 0.0 6 174 4 168 266 0.0 6 259 6 0 0 1 169 163 0.0 6 162 1 170 121 0.0 6 120 1 171 200 0.0 6 195 5 172 175 0.0 6 171 3 0 0 0 0 1 173 170 0.0 6 164 6 174 159 0.0 6 155 4 175 261 0.0 6 254 7 176 228 0.0 6 224 4 177 239 0.0 6 237 1 0 0 1 178 158 0.0 6 148 10 179 227 0.0 6 221 6 180 191 0.0 6 183 7 0 0 0 0 1 181 293 0.0 6 285 7 0 1 182 160 0.0 6 158 2 183 206 0.0 6 203 3 184 120 0.0 6 115 5 185 127 0.0 6 124 3 186 272 0.0 6 270 2 187 407 0.0 6 399 7 1 188 408 0.0 6 391 14 1 0 2 189 363 0.0 6 353 10 190 352 0.0 6 340 10 1 0 1 191 276 0.0 6 267 9 192 242 0.0 6 236 5 1 193 281 0.0 6 272 8 0 0 1 194 272 0.0 6 265 7 195 225 0.0 6 220 5 196 373 0.0 6 364 9 197 189 0.0 6 186 3 198 397 0.0 6 385 12 199 319 0.0 6 305 14 200 253 0.0 6 244 9 201 345 0.0 6 334 10 0 0 1 202 272 0.0 6 258 12 1 0 1 203 169 0.0 6 164 5 204 229 0.0 6 224 5 205 210 0.0 6 208 2 206 116 0.0 6 107 8 1 207 131 0.0 6 127 4 208 264 0.0 6 254 8 1 0 1 209 80 0.0 6 77 2 0 1 210 120 0.0 6 117 3 211 230 0.0 6 220 10 212 174 0.0 6 170 4 213 77 0.0 6 75 2 214 170 0.0 6 167 3 215 185 0.0 6 176 9 216 149 0.0 6 144 4 0 0 1 217 106 0.0 6 101 5 218 75 0.0 6 72 3 219 87 0.0 6 83 4 220 62 0.0 6 62 221 82 0.0 6 82 222 58 0.0 6 56 2 223 87 0.0 6 83 4 224 105 0.0 6 103 2 225 85 0.0 6 79 5 0 0 1 226 118 0.0 6 115 3 227 300 0.0 6 286 12 1 0 1 228 128 0.0 6 121 6 0 1 229 197 0.0 6 192 5 230 292 0.0 6 282 10 231 145 0.0 6 140 5 232 283 0.0 6 273 10 233 53 0.0 6 52 0 0 0 1 234 109 0.0 6 101 8 235 84 0.0 6 82 1 0 0 1 236 52 0.0 6 52 237 61 0.0 6 59 2 238 44 0.0 6 43 1 239 17 0.0 6 17 240 22 0.0 6 21 1 241 35 0.0 6 34 1 242 46 0.0 6 46 243 22 0.0 6 22 244 27 0.0 6 26 1 245 83 0.0 6 79 4 246 51 0.0 6 47 3 1 247 39 0.0 6 39 248 34 0.0 6 30 4 249 43 0.0 6 41 2 250 28 0.0 6 27 1 251 82 0.0 6 78 4 252 38 0.0 6 37 1 253 16 0.0 6 14 2 254 75 0.0 6 69 6 255 12 0.0 6 12 256 11 0.0 6 11 257 4 0.0 6 4 258 2 0.0 6 2 265 1 0.0 6 1 269 2 0.0 6 2 279 2 0.0 6 2 280 1 0.0 6 1 281 17 0.0 6 15 2 282 41 0.0 6 40 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 240 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 91 2 0.0 6 2 92 10 0.0 6 10 96 1 0.0 6 0 0 0 0 1 103 1 0.0 6 1 105 1 0.0 6 1 106 1 0.0 6 1 107 2 0.0 6 2 116 1 0.0 6 1 118 5 0.0 6 5 119 23 0.0 6 23 123 1 0.0 6 0 0 0 0 1 124 1 0.0 6 1 125 6 0.0 6 6 131 2 0.0 6 2 133 3 0.0 6 3 134 4 0.0 6 4 135 7 0.0 6 7 136 24 0.0 6 24 137 1 0.0 6 1 144 4 0.0 6 4 145 17 0.0 6 17 146 1 0.0 6 1 148 2 0.0 6 2 149 17 0.0 6 17 152 1 0.0 6 1 155 1 0.0 6 1 167 1 0.0 6 1 168 2 0.0 6 2 170 7 0.0 6 7 171 56 0.0 6 56 175 1 0.0 6 1 177 2 0.0 6 2 178 20 0.0 6 20 182 1 0.0 6 0 0 0 0 1 193 1 0.0 6 1 194 8 0.0 6 8 206 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_05_4_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_05_231_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_05_4_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_05_231_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.27 s (28 µs/read; 2.13 M reads/minute). === Summary === Total read pairs processed: 80,426 Read 1 with adapter: 80,424 (100.0%) Read 2 with adapter: 80,373 (99.9%) == Read fate breakdown == Pairs that were too short: 25 (0.0%) Pairs discarded as untrimmed: 55 (0.1%) Pairs written (passing filters): 80,346 (99.9%) Total basepairs processed: 34,312,282 bp Read 1: 19,258,579 bp Read 2: 15,053,703 bp Total written (filtered): 5,043,424 bp (14.7%) Read 1: 2,581,229 bp Read 2: 2,462,195 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 78578 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 1256.7 0 2 1 4 1 314.2 1 0 1 6 2 19.6 1 0 0 2 7 1 4.9 2 0 0 1 8 2 1.2 2 2 9 2 0.3 2 2 10 2 0.1 3 2 15 3 0.0 4 2 1 16 7 0.0 4 5 2 17 2 0.0 5 2 20 135 0.0 6 134 1 21 67 0.0 6 66 1 22 60 0.0 6 60 23 49 0.0 6 48 1 24 23 0.0 6 23 25 25 0.0 6 25 26 20 0.0 6 17 2 1 27 11 0.0 6 11 28 6 0.0 6 6 29 12 0.0 6 12 30 9 0.0 6 9 31 6 0.0 6 6 32 7 0.0 6 6 0 0 1 33 5 0.0 6 5 34 4 0.0 6 4 35 9 0.0 6 9 36 4 0.0 6 4 37 8 0.0 6 8 38 9 0.0 6 9 39 11 0.0 6 11 40 5 0.0 6 5 41 5 0.0 6 5 42 5 0.0 6 5 43 6 0.0 6 6 44 5 0.0 6 5 45 8 0.0 6 7 1 46 4 0.0 6 4 47 8 0.0 6 8 48 8 0.0 6 7 1 49 6 0.0 6 5 0 0 0 1 50 4 0.0 6 4 51 9 0.0 6 9 52 6 0.0 6 6 53 10 0.0 6 10 54 4 0.0 6 3 1 55 3 0.0 6 3 56 4 0.0 6 4 57 8 0.0 6 8 58 3 0.0 6 2 1 59 1 0.0 6 1 60 3 0.0 6 3 61 7 0.0 6 7 62 6 0.0 6 6 63 6 0.0 6 6 64 5 0.0 6 5 65 7 0.0 6 7 66 12 0.0 6 12 67 15 0.0 6 14 1 68 10 0.0 6 8 2 69 9 0.0 6 9 70 11 0.0 6 11 71 18 0.0 6 16 2 72 9 0.0 6 8 1 73 13 0.0 6 12 1 74 9 0.0 6 9 75 12 0.0 6 12 76 14 0.0 6 13 1 77 9 0.0 6 9 78 8 0.0 6 8 79 5 0.0 6 5 80 7 0.0 6 7 81 11 0.0 6 11 82 10 0.0 6 9 1 83 6 0.0 6 6 84 18 0.0 6 16 1 0 0 0 1 85 15 0.0 6 14 1 86 14 0.0 6 14 87 11 0.0 6 11 88 11 0.0 6 10 0 0 0 0 1 89 6 0.0 6 6 90 15 0.0 6 15 91 13 0.0 6 13 92 7 0.0 6 7 93 7 0.0 6 7 94 8 0.0 6 8 95 7 0.0 6 7 96 7 0.0 6 7 97 12 0.0 6 10 2 98 13 0.0 6 13 99 12 0.0 6 12 100 10 0.0 6 9 0 1 101 10 0.0 6 10 102 9 0.0 6 8 1 103 12 0.0 6 12 104 23 0.0 6 22 1 105 22 0.0 6 21 1 106 22 0.0 6 22 107 21 0.0 6 21 108 17 0.0 6 16 1 109 21 0.0 6 21 110 14 0.0 6 14 111 25 0.0 6 25 112 22 0.0 6 21 1 113 27 0.0 6 26 0 1 114 29 0.0 6 29 115 34 0.0 6 33 0 0 1 116 36 0.0 6 36 117 21 0.0 6 21 118 26 0.0 6 24 1 0 0 0 1 119 23 0.0 6 22 1 120 29 0.0 6 28 1 121 21 0.0 6 17 2 1 0 0 0 1 122 30 0.0 6 30 123 36 0.0 6 34 2 124 18 0.0 6 18 125 35 0.0 6 34 1 126 32 0.0 6 32 127 21 0.0 6 20 1 128 21 0.0 6 21 129 44 0.0 6 43 1 130 42 0.0 6 40 2 131 31 0.0 6 31 132 36 0.0 6 35 1 133 45 0.0 6 43 2 134 57 0.0 6 56 1 135 68 0.0 6 68 136 41 0.0 6 41 137 40 0.0 6 39 1 138 55 0.0 6 55 139 56 0.0 6 55 1 140 84 0.0 6 83 1 141 221 0.0 6 221 142 365 0.0 6 364 1 143 464 0.0 6 464 144 447 0.0 6 445 2 145 481 0.0 6 481 146 531 0.0 6 531 147 507 0.0 6 507 148 638 0.0 6 637 1 149 602 0.0 6 600 2 150 654 0.0 6 652 2 151 789 0.0 6 789 152 504 0.0 6 502 1 1 153 388 0.0 6 386 2 154 405 0.0 6 402 3 155 378 0.0 6 376 2 156 389 0.0 6 388 1 157 369 0.0 6 368 1 158 427 0.0 6 427 159 389 0.0 6 389 160 387 0.0 6 387 161 441 0.0 6 439 2 162 402 0.0 6 401 0 0 0 0 1 163 392 0.0 6 392 164 401 0.0 6 398 3 165 340 0.0 6 337 1 0 0 1 0 1 166 296 0.0 6 295 0 0 0 0 1 167 344 0.0 6 341 3 168 341 0.0 6 340 1 169 350 0.0 6 347 1 0 0 1 0 1 170 362 0.0 6 360 2 171 394 0.0 6 393 1 172 394 0.0 6 394 173 436 0.0 6 435 1 174 395 0.0 6 394 1 175 324 0.0 6 324 176 396 0.0 6 395 1 177 350 0.0 6 347 3 178 374 0.0 6 369 3 0 0 0 1 1 179 532 0.0 6 530 2 180 413 0.0 6 411 1 0 0 0 0 1 181 314 0.0 6 310 3 0 0 1 182 370 0.0 6 369 1 183 415 0.0 6 413 2 184 388 0.0 6 384 4 185 426 0.0 6 420 6 186 418 0.0 6 414 4 187 366 0.0 6 365 1 188 409 0.0 6 404 5 189 399 0.0 6 396 2 1 190 377 0.0 6 371 6 191 430 0.0 6 429 1 192 520 0.0 6 517 3 193 552 0.0 6 547 4 0 0 0 0 1 194 506 0.0 6 501 5 195 558 0.0 6 554 3 0 0 0 1 196 656 0.0 6 653 3 197 743 0.0 6 740 2 0 0 0 0 1 198 801 0.0 6 795 5 0 0 0 0 1 199 908 0.0 6 901 6 0 0 1 200 749 0.0 6 745 3 0 0 0 1 201 642 0.0 6 637 4 1 202 681 0.0 6 671 9 0 1 203 672 0.0 6 669 3 204 690 0.0 6 687 2 0 0 0 1 205 672 0.0 6 668 3 0 0 0 1 206 733 0.0 6 724 8 1 207 941 0.0 6 936 4 0 0 0 1 208 726 0.0 6 725 1 209 874 0.0 6 869 4 1 210 645 0.0 6 642 3 211 677 0.0 6 672 5 212 829 0.0 6 822 6 0 0 0 0 1 213 873 0.0 6 869 3 1 214 840 0.0 6 831 8 0 1 215 1045 0.0 6 1030 13 2 216 1181 0.0 6 1177 4 217 847 0.0 6 840 6 0 0 0 0 1 218 954 0.0 6 949 5 219 962 0.0 6 955 6 0 0 0 1 220 971 0.0 6 966 4 0 0 0 1 221 831 0.0 6 824 6 0 0 0 1 222 721 0.0 6 718 3 223 762 0.0 6 759 3 224 817 0.0 6 808 8 0 0 0 1 225 902 0.0 6 895 7 226 972 0.0 6 966 4 0 1 0 0 1 227 943 0.0 6 935 8 228 776 0.0 6 772 3 1 229 721 0.0 6 718 2 0 0 0 0 1 230 811 0.0 6 808 2 0 0 1 231 749 0.0 6 745 3 0 1 232 788 0.0 6 785 3 233 794 0.0 6 790 4 234 768 0.0 6 759 7 2 235 692 0.0 6 686 6 236 691 0.0 6 688 3 237 763 0.0 6 759 2 0 0 1 1 238 649 0.0 6 644 5 239 644 0.0 6 643 0 1 240 570 0.0 6 568 1 1 241 588 0.0 6 585 3 242 604 0.0 6 601 3 243 670 0.0 6 666 4 244 557 0.0 6 555 1 0 0 0 0 1 245 540 0.0 6 539 1 246 475 0.0 6 474 1 247 418 0.0 6 416 2 248 406 0.0 6 404 1 0 0 0 0 1 249 454 0.0 6 452 1 1 250 385 0.0 6 383 1 0 1 251 429 0.0 6 426 3 252 408 0.0 6 405 3 253 374 0.0 6 371 3 254 398 0.0 6 394 4 255 347 0.0 6 347 256 333 0.0 6 328 5 257 331 0.0 6 329 2 258 372 0.0 6 369 3 259 334 0.0 6 332 2 260 378 0.0 6 377 1 261 355 0.0 6 351 3 1 262 256 0.0 6 253 2 1 263 284 0.0 6 283 1 264 262 0.0 6 257 4 0 0 1 265 261 0.0 6 260 1 266 244 0.0 6 242 2 267 214 0.0 6 213 1 268 216 0.0 6 216 269 223 0.0 6 220 2 1 270 310 0.0 6 309 1 271 227 0.0 6 227 272 186 0.0 6 185 1 273 182 0.0 6 178 2 1 0 1 274 228 0.0 6 227 1 275 249 0.0 6 248 1 276 304 0.0 6 302 2 277 342 0.0 6 342 278 455 0.0 6 453 1 0 1 279 1405 0.0 6 1400 4 0 1 280 2135 0.0 6 2114 19 1 0 0 0 1 281 17 0.0 6 17 282 2 0.0 6 2 283 2 0.0 6 2 286 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1846 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 3 0.0 6 1 0 2 61 5 0.0 6 2 2 1 62 151 0.0 6 147 4 63 4 0.0 6 4 64 4 0.0 6 4 65 1 0.0 6 1 66 1 0.0 6 1 68 1 0.0 6 1 70 4 0.0 6 4 71 4 0.0 6 4 72 13 0.0 6 13 73 18 0.0 6 18 74 11 0.0 6 11 75 6 0.0 6 6 76 12 0.0 6 12 77 15 0.0 6 15 78 19 0.0 6 19 79 23 0.0 6 23 80 18 0.0 6 18 81 24 0.0 6 24 82 10 0.0 6 10 83 9 0.0 6 9 84 9 0.0 6 9 85 8 0.0 6 8 86 16 0.0 6 16 87 11 0.0 6 11 88 8 0.0 6 7 0 0 0 0 0 1 89 6 0.0 6 6 90 10 0.0 6 10 91 11 0.0 6 11 92 6 0.0 6 6 93 5 0.0 6 5 94 10 0.0 6 10 95 6 0.0 6 6 96 5 0.0 6 5 97 9 0.0 6 9 98 9 0.0 6 9 99 9 0.0 6 9 100 8 0.0 6 8 101 13 0.0 6 13 102 6 0.0 6 6 103 9 0.0 6 9 104 11 0.0 6 11 105 9 0.0 6 9 106 8 0.0 6 8 107 6 0.0 6 6 108 12 0.0 6 12 109 22 0.0 6 22 110 5 0.0 6 5 111 5 0.0 6 5 112 9 0.0 6 9 113 10 0.0 6 10 114 10 0.0 6 10 115 3 0.0 6 3 116 8 0.0 6 8 117 16 0.0 6 16 118 6 0.0 6 6 119 6 0.0 6 6 120 6 0.0 6 6 121 12 0.0 6 12 122 4 0.0 6 4 123 5 0.0 6 5 124 5 0.0 6 5 125 8 0.0 6 8 126 4 0.0 6 4 127 10 0.0 6 10 128 5 0.0 6 5 129 5 0.0 6 5 130 11 0.0 6 11 131 6 0.0 6 6 132 7 0.0 6 7 133 11 0.0 6 11 134 10 0.0 6 10 135 12 0.0 6 12 136 9 0.0 6 9 137 8 0.0 6 8 138 11 0.0 6 11 139 9 0.0 6 9 140 14 0.0 6 14 141 5 0.0 6 5 142 7 0.0 6 7 143 7 0.0 6 7 144 15 0.0 6 15 145 7 0.0 6 7 146 9 0.0 6 9 147 5 0.0 6 5 148 5 0.0 6 5 149 6 0.0 6 6 150 10 0.0 6 10 151 13 0.0 6 13 152 7 0.0 6 7 153 12 0.0 6 12 154 4 0.0 6 4 155 12 0.0 6 12 156 11 0.0 6 11 157 14 0.0 6 14 158 6 0.0 6 6 159 10 0.0 6 10 160 10 0.0 6 10 161 7 0.0 6 7 162 6 0.0 6 6 163 5 0.0 6 5 164 6 0.0 6 6 165 15 0.0 6 15 166 11 0.0 6 11 167 12 0.0 6 12 168 17 0.0 6 17 169 6 0.0 6 6 170 4 0.0 6 4 171 8 0.0 6 8 172 5 0.0 6 5 173 6 0.0 6 6 174 13 0.0 6 13 175 9 0.0 6 9 176 9 0.0 6 9 177 10 0.0 6 10 178 8 0.0 6 8 179 8 0.0 6 8 180 11 0.0 6 11 181 13 0.0 6 13 182 4 0.0 6 4 183 14 0.0 6 14 184 11 0.0 6 11 185 12 0.0 6 12 186 10 0.0 6 10 187 15 0.0 6 15 188 13 0.0 6 13 189 15 0.0 6 15 190 13 0.0 6 13 191 8 0.0 6 8 192 10 0.0 6 10 193 5 0.0 6 5 194 7 0.0 6 7 195 5 0.0 6 5 196 11 0.0 6 11 197 7 0.0 6 7 198 8 0.0 6 8 199 9 0.0 6 9 200 5 0.0 6 5 201 10 0.0 6 10 202 21 0.0 6 21 203 7 0.0 6 7 204 7 0.0 6 7 205 5 0.0 6 5 206 6 0.0 6 6 207 7 0.0 6 7 208 10 0.0 6 10 209 5 0.0 6 5 210 9 0.0 6 9 211 15 0.0 6 14 0 0 0 0 0 1 212 12 0.0 6 12 213 8 0.0 6 8 214 7 0.0 6 7 215 6 0.0 6 6 216 10 0.0 6 10 217 9 0.0 6 9 218 6 0.0 6 6 219 7 0.0 6 7 220 8 0.0 6 8 221 7 0.0 6 7 222 8 0.0 6 8 223 7 0.0 6 7 224 11 0.0 6 11 225 10 0.0 6 10 226 8 0.0 6 8 227 8 0.0 6 8 228 6 0.0 6 6 229 10 0.0 6 10 230 11 0.0 6 11 231 9 0.0 6 9 232 7 0.0 6 7 233 5 0.0 6 5 234 5 0.0 6 5 235 11 0.0 6 11 236 10 0.0 6 10 237 3 0.0 6 3 238 3 0.0 6 3 239 3 0.0 6 3 240 8 0.0 6 8 241 1 0.0 6 1 242 7 0.0 6 7 243 5 0.0 6 5 244 4 0.0 6 4 245 2 0.0 6 2 246 6 0.0 6 6 247 2 0.0 6 2 248 2 0.0 6 2 249 2 0.0 6 2 250 4 0.0 6 4 251 4 0.0 6 4 252 3 0.0 6 3 253 7 0.0 6 7 254 1 0.0 6 1 255 3 0.0 6 3 256 3 0.0 6 3 257 7 0.0 6 7 258 2 0.0 6 2 259 3 0.0 6 3 260 3 0.0 6 3 261 4 0.0 6 4 262 1 0.0 6 1 263 2 0.0 6 2 264 3 0.0 6 3 266 3 0.0 6 3 267 1 0.0 6 1 269 3 0.0 6 3 270 3 0.0 6 3 271 1 0.0 6 1 273 5 0.0 6 5 274 4 0.0 6 4 276 1 0.0 6 1 277 1 0.0 6 1 278 3 0.0 6 3 279 3 0.0 6 3 280 1 0.0 6 1 281 1 0.0 6 1 282 1 0.0 6 1 284 1 0.0 6 1 285 1 0.0 6 1 286 1 0.0 6 1 289 1 0.0 6 1 291 1 0.0 6 1 292 1 0.0 6 1 294 2 0.0 6 2 295 3 0.0 6 3 296 1 0.0 6 1 298 1 0.0 6 1 308 1 0.0 6 1 309 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 78856 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 314.2 1 1 6 1 19.6 1 0 1 7 1 4.9 2 1 9 1 0.3 2 1 11 1 0.0 3 1 12 6 0.0 3 5 0 0 1 13 16 0.0 3 15 1 14 9 0.0 4 8 0 0 1 15 3 0.0 4 3 16 20 0.0 4 17 3 17 33 0.0 5 32 0 1 18 13 0.0 5 11 2 19 17 0.0 5 15 2 20 152 0.0 6 149 2 1 21 70 0.0 6 66 4 22 44 0.0 6 41 3 23 31 0.0 6 30 0 0 1 24 18 0.0 6 17 1 25 26 0.0 6 26 26 26 0.0 6 25 1 27 17 0.0 6 16 1 28 31 0.0 6 29 2 29 14 0.0 6 12 1 1 30 18 0.0 6 17 1 31 20 0.0 6 19 0 1 32 14 0.0 6 13 1 33 23 0.0 6 23 34 15 0.0 6 14 1 35 18 0.0 6 18 36 18 0.0 6 17 1 37 10 0.0 6 9 1 38 19 0.0 6 19 39 12 0.0 6 9 3 40 19 0.0 6 18 1 41 17 0.0 6 14 1 1 0 0 1 42 16 0.0 6 13 2 0 0 0 0 1 43 18 0.0 6 18 44 18 0.0 6 17 1 45 21 0.0 6 21 46 17 0.0 6 14 3 47 25 0.0 6 19 5 0 1 48 17 0.0 6 16 1 49 31 0.0 6 31 50 26 0.0 6 22 3 1 51 25 0.0 6 23 2 52 25 0.0 6 22 3 53 35 0.0 6 33 2 54 24 0.0 6 24 55 29 0.0 6 28 0 1 56 19 0.0 6 18 1 57 28 0.0 6 25 3 58 26 0.0 6 24 2 59 32 0.0 6 29 3 60 30 0.0 6 27 3 61 30 0.0 6 28 2 62 24 0.0 6 24 63 35 0.0 6 33 2 64 32 0.0 6 30 1 1 65 36 0.0 6 35 1 66 34 0.0 6 32 1 0 1 67 34 0.0 6 34 68 57 0.0 6 51 2 0 1 1 1 1 69 167 0.0 6 161 5 1 70 76 0.0 6 72 3 0 1 71 87 0.0 6 79 4 2 0 2 72 52 0.0 6 49 2 0 1 73 62 0.0 6 60 2 74 55 0.0 6 52 3 75 48 0.0 6 46 1 1 76 62 0.0 6 62 77 39 0.0 6 35 3 0 0 1 78 51 0.0 6 50 0 1 79 50 0.0 6 48 0 1 0 0 1 80 49 0.0 6 47 1 0 0 0 0 1 81 42 0.0 6 40 2 82 62 0.0 6 59 3 83 66 0.0 6 62 4 84 74 0.0 6 72 1 0 0 0 0 1 85 88 0.0 6 85 3 86 50 0.0 6 48 1 0 1 87 48 0.0 6 45 2 0 0 0 1 88 62 0.0 6 62 89 57 0.0 6 57 90 62 0.0 6 59 3 91 60 0.0 6 57 2 0 0 0 1 92 98 0.0 6 94 2 0 0 0 1 1 93 72 0.0 6 68 4 94 99 0.0 6 94 3 2 95 105 0.0 6 99 4 1 1 96 85 0.0 6 83 2 97 87 0.0 6 85 1 0 0 0 1 98 120 0.0 6 118 1 0 1 99 109 0.0 6 106 3 100 102 0.0 6 98 4 101 92 0.0 6 89 1 0 2 102 99 0.0 6 97 2 103 130 0.0 6 125 5 104 116 0.0 6 114 1 0 0 0 0 1 105 145 0.0 6 142 3 106 200 0.0 6 196 4 107 135 0.0 6 130 4 0 0 0 0 1 108 117 0.0 6 114 2 0 0 1 109 147 0.0 6 143 4 110 146 0.0 6 140 4 1 0 0 1 111 130 0.0 6 128 2 112 160 0.0 6 152 6 2 113 161 0.0 6 152 7 1 0 0 1 114 203 0.0 6 195 5 1 0 1 1 115 170 0.0 6 163 7 116 195 0.0 6 191 3 0 0 0 1 117 192 0.0 6 189 3 118 201 0.0 6 194 4 1 0 1 0 1 119 215 0.0 6 211 3 1 120 202 0.0 6 198 4 121 212 0.0 6 205 7 122 209 0.0 6 202 6 0 0 1 123 226 0.0 6 223 3 124 229 0.0 6 221 6 0 1 0 0 1 125 264 0.0 6 258 6 126 291 0.0 6 283 6 0 1 0 0 1 127 362 0.0 6 350 10 1 0 0 0 1 128 312 0.0 6 300 9 3 129 363 0.0 6 358 4 0 0 0 0 1 130 497 0.0 6 489 5 0 1 2 131 428 0.0 6 426 1 0 0 0 0 1 132 456 0.0 6 448 5 1 1 1 133 493 0.0 6 479 11 1 0 0 2 134 476 0.0 6 466 9 1 135 496 0.0 6 488 7 0 1 136 603 0.0 6 593 9 0 0 0 0 1 137 577 0.0 6 565 8 1 1 0 0 2 138 583 0.0 6 573 8 0 0 0 0 2 139 695 0.0 6 684 10 0 0 0 1 140 760 0.0 6 752 7 0 0 0 1 141 901 0.0 6 894 6 0 0 0 1 142 967 0.0 6 953 13 0 1 143 1020 0.0 6 1010 8 1 0 0 1 144 1152 0.0 6 1138 11 0 2 0 0 1 145 1145 0.0 6 1128 14 0 2 0 1 146 1316 0.0 6 1295 16 3 1 0 0 1 147 1215 0.0 6 1200 14 1 148 1383 0.0 6 1367 11 2 1 0 0 2 149 1506 0.0 6 1492 9 1 0 2 1 1 150 1379 0.0 6 1363 12 0 2 1 1 151 1333 0.0 6 1313 17 1 1 0 1 152 1868 0.0 6 1832 29 2 1 0 1 3 153 1448 0.0 6 1421 22 1 1 0 0 3 154 1716 0.0 6 1686 25 1 1 1 1 1 155 1588 0.0 6 1564 19 3 0 0 1 1 156 1321 0.0 6 1310 10 0 1 157 1493 0.0 6 1471 19 0 1 1 1 158 1305 0.0 6 1289 13 0 0 0 1 2 159 1355 0.0 6 1329 21 1 2 1 0 1 160 1366 0.0 6 1352 12 0 2 161 1222 0.0 6 1206 13 0 1 1 1 162 1188 0.0 6 1168 13 2 1 2 1 1 163 1104 0.0 6 1086 14 1 0 1 0 2 164 980 0.0 6 963 12 2 0 0 2 1 165 1120 0.0 6 1106 11 0 1 1 0 1 166 968 0.0 6 950 16 1 1 167 1008 0.0 6 1001 4 1 0 1 0 1 168 1112 0.0 6 1096 14 0 0 1 0 1 169 982 0.0 6 971 10 0 1 170 1213 0.0 6 1201 8 1 0 1 1 1 171 1016 0.0 6 995 19 0 0 2 172 1066 0.0 6 1050 12 0 2 0 2 173 1122 0.0 6 1111 9 1 1 174 1131 0.0 6 1122 7 0 1 0 0 1 175 1065 0.0 6 1053 10 0 0 0 2 176 1019 0.0 6 1008 8 0 0 0 3 177 1007 0.0 6 995 10 1 0 1 178 987 0.0 6 975 11 0 0 0 1 179 871 0.0 6 860 10 0 0 0 0 1 180 857 0.0 6 850 4 0 2 0 1 181 743 0.0 6 736 5 0 1 0 0 1 182 657 0.0 6 650 7 183 633 0.0 6 629 4 184 602 0.0 6 592 8 0 1 0 1 185 880 0.0 6 867 11 0 0 1 1 186 1054 0.0 6 1047 7 187 919 0.0 6 912 7 188 689 0.0 6 678 9 2 189 589 0.0 6 586 3 190 609 0.0 6 605 4 191 584 0.0 6 576 7 0 0 1 192 524 0.0 6 516 7 0 0 1 193 557 0.0 6 550 6 0 0 0 1 194 428 0.0 6 424 4 195 412 0.0 6 403 8 1 196 351 0.0 6 349 2 197 356 0.0 6 353 3 198 461 0.0 6 452 8 1 199 345 0.0 6 343 2 200 300 0.0 6 300 201 302 0.0 6 297 1 0 1 1 2 202 304 0.0 6 299 2 1 2 203 234 0.0 6 231 3 204 256 0.0 6 255 0 0 0 1 205 219 0.0 6 215 4 206 179 0.0 6 177 2 207 162 0.0 6 161 1 208 174 0.0 6 173 1 209 177 0.0 6 175 2 210 149 0.0 6 149 211 136 0.0 6 135 1 212 110 0.0 6 108 1 0 0 0 1 213 102 0.0 6 101 1 214 118 0.0 6 118 215 111 0.0 6 110 1 216 142 0.0 6 141 1 217 74 0.0 6 74 218 80 0.0 6 79 1 219 81 0.0 6 81 220 75 0.0 6 73 2 221 54 0.0 6 54 222 45 0.0 6 45 223 60 0.0 6 57 3 224 42 0.0 6 40 2 225 38 0.0 6 38 226 34 0.0 6 33 1 227 31 0.0 6 31 228 24 0.0 6 24 229 20 0.0 6 20 230 24 0.0 6 24 231 12 0.0 6 12 232 13 0.0 6 13 233 14 0.0 6 13 1 234 15 0.0 6 15 235 15 0.0 6 15 236 12 0.0 6 12 237 3 0.0 6 2 1 238 4 0.0 6 4 239 4 0.0 6 4 240 3 0.0 6 3 241 3 0.0 6 3 242 5 0.0 6 5 243 5 0.0 6 5 244 3 0.0 6 3 245 3 0.0 6 3 246 1 0.0 6 1 247 1 0.0 6 1 248 1 0.0 6 1 249 1 0.0 6 1 254 1 0.0 6 1 277 2 0.0 6 2 279 4 0.0 6 4 280 12 0.0 6 11 1 281 121 0.0 6 120 1 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1517 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 47 1 0.0 6 0 1 51 2 0.0 6 2 52 4 0.0 6 4 53 3 0.0 6 3 54 1 0.0 6 1 59 3 0.0 6 3 61 4 0.0 6 4 62 3 0.0 6 2 1 63 21 0.0 6 21 64 3 0.0 6 3 65 1 0.0 6 0 0 0 0 1 66 1 0.0 6 1 68 4 0.0 6 4 69 24 0.0 6 23 1 70 15 0.0 6 15 71 9 0.0 6 9 72 15 0.0 6 15 73 11 0.0 6 11 74 9 0.0 6 9 75 28 0.0 6 28 76 11 0.0 6 11 77 25 0.0 6 25 78 18 0.0 6 18 79 35 0.0 6 34 1 80 31 0.0 6 31 81 22 0.0 6 22 82 22 0.0 6 22 83 15 0.0 6 15 84 10 0.0 6 10 85 10 0.0 6 10 86 8 0.0 6 8 87 21 0.0 6 21 88 15 0.0 6 15 89 10 0.0 6 10 90 20 0.0 6 20 91 15 0.0 6 15 92 8 0.0 6 8 93 12 0.0 6 12 94 7 0.0 6 7 95 8 0.0 6 8 96 10 0.0 6 10 97 12 0.0 6 12 98 12 0.0 6 12 99 9 0.0 6 9 100 15 0.0 6 15 101 15 0.0 6 14 1 102 18 0.0 6 17 0 0 0 1 103 11 0.0 6 11 104 9 0.0 6 9 105 12 0.0 6 12 106 6 0.0 6 6 107 12 0.0 6 12 108 19 0.0 6 19 109 7 0.0 6 7 110 3 0.0 6 3 111 11 0.0 6 11 112 9 0.0 6 9 113 6 0.0 6 6 114 8 0.0 6 8 115 7 0.0 6 7 116 5 0.0 6 5 117 11 0.0 6 11 118 8 0.0 6 8 119 6 0.0 6 6 120 8 0.0 6 8 121 9 0.0 6 9 122 7 0.0 6 7 123 7 0.0 6 7 124 9 0.0 6 8 0 0 0 1 125 7 0.0 6 7 126 10 0.0 6 10 127 9 0.0 6 7 0 1 0 1 128 8 0.0 6 8 129 7 0.0 6 7 130 10 0.0 6 10 131 17 0.0 6 17 132 13 0.0 6 13 133 10 0.0 6 10 134 9 0.0 6 9 135 7 0.0 6 7 136 5 0.0 6 5 137 8 0.0 6 8 138 10 0.0 6 10 139 13 0.0 6 13 140 4 0.0 6 4 141 6 0.0 6 6 142 7 0.0 6 7 143 9 0.0 6 8 1 144 9 0.0 6 9 145 9 0.0 6 9 146 2 0.0 6 2 147 9 0.0 6 9 148 9 0.0 6 9 149 9 0.0 6 9 150 18 0.0 6 18 151 13 0.0 6 13 152 7 0.0 6 7 153 7 0.0 6 7 154 13 0.0 6 13 155 4 0.0 6 4 156 12 0.0 6 11 1 157 7 0.0 6 7 158 17 0.0 6 17 159 6 0.0 6 6 160 10 0.0 6 10 161 13 0.0 6 13 162 12 0.0 6 12 163 8 0.0 6 8 164 15 0.0 6 14 1 165 25 0.0 6 25 166 14 0.0 6 14 167 5 0.0 6 5 168 13 0.0 6 13 169 13 0.0 6 13 170 5 0.0 6 5 171 10 0.0 6 10 172 8 0.0 6 8 173 5 0.0 6 5 174 8 0.0 6 8 175 10 0.0 6 10 176 6 0.0 6 6 177 9 0.0 6 9 178 9 0.0 6 9 179 10 0.0 6 10 180 5 0.0 6 5 181 6 0.0 6 6 182 9 0.0 6 9 183 8 0.0 6 8 184 7 0.0 6 7 185 2 0.0 6 2 186 15 0.0 6 15 187 6 0.0 6 6 188 6 0.0 6 6 189 10 0.0 6 10 190 6 0.0 6 6 191 6 0.0 6 6 192 10 0.0 6 10 193 9 0.0 6 9 194 3 0.0 6 3 195 5 0.0 6 5 196 4 0.0 6 4 197 6 0.0 6 6 198 4 0.0 6 4 199 14 0.0 6 14 200 5 0.0 6 5 201 8 0.0 6 8 202 6 0.0 6 6 203 5 0.0 6 4 1 204 4 0.0 6 4 205 3 0.0 6 3 206 3 0.0 6 3 207 2 0.0 6 2 208 4 0.0 6 4 209 2 0.0 6 2 210 2 0.0 6 2 211 3 0.0 6 3 212 2 0.0 6 1 1 213 1 0.0 6 1 214 1 0.0 6 1 215 4 0.0 6 4 216 3 0.0 6 3 217 3 0.0 6 3 218 2 0.0 6 2 219 3 0.0 6 3 221 2 0.0 6 2 222 2 0.0 6 2 223 1 0.0 6 1 226 2 0.0 6 2 229 1 0.0 6 1 230 2 0.0 6 2 231 2 0.0 6 2 232 1 0.0 6 1 233 2 0.0 6 2 241 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_06_5_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_06_232_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_06_5_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_06_232_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.24 s (23 µs/read; 2.59 M reads/minute). === Summary === Total read pairs processed: 96,918 Read 1 with adapter: 96,909 (100.0%) Read 2 with adapter: 96,875 (100.0%) == Read fate breakdown == Pairs that were too short: 6 (0.0%) Pairs discarded as untrimmed: 52 (0.1%) Pairs written (passing filters): 96,860 (99.9%) Total basepairs processed: 45,745,767 bp Read 1: 26,121,642 bp Read 2: 19,624,125 bp Total written (filtered): 5,862,392 bp (12.8%) Read 1: 3,034,019 bp Read 2: 2,828,373 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 96229 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 1514.3 0 1 2 4 1 378.6 1 1 6 1 23.7 1 0 0 1 7 2 5.9 2 1 0 1 14 1 0.0 4 1 15 1 0.0 4 1 16 7 0.0 4 5 2 17 3 0.0 5 3 18 1 0.0 5 1 19 1 0.0 5 1 20 74 0.0 6 71 3 21 39 0.0 6 38 1 22 24 0.0 6 24 23 23 0.0 6 22 1 24 18 0.0 6 16 2 25 19 0.0 6 19 26 10 0.0 6 10 27 3 0.0 6 3 28 5 0.0 6 5 29 11 0.0 6 11 30 2 0.0 6 2 31 2 0.0 6 2 32 3 0.0 6 2 0 0 0 0 0 1 33 4 0.0 6 4 34 2 0.0 6 2 35 3 0.0 6 3 36 3 0.0 6 3 37 2 0.0 6 2 38 7 0.0 6 7 39 4 0.0 6 4 40 6 0.0 6 6 41 4 0.0 6 4 42 2 0.0 6 2 43 7 0.0 6 6 1 44 7 0.0 6 6 1 45 1 0.0 6 1 46 1 0.0 6 1 47 4 0.0 6 4 48 4 0.0 6 4 49 3 0.0 6 2 1 50 5 0.0 6 4 1 51 4 0.0 6 4 52 8 0.0 6 7 1 53 3 0.0 6 3 54 5 0.0 6 5 55 4 0.0 6 4 56 3 0.0 6 3 57 3 0.0 6 3 58 4 0.0 6 4 59 6 0.0 6 4 2 60 6 0.0 6 5 1 62 5 0.0 6 5 64 3 0.0 6 3 65 5 0.0 6 4 1 66 2 0.0 6 2 67 6 0.0 6 6 68 6 0.0 6 6 69 2 0.0 6 2 70 3 0.0 6 3 71 3 0.0 6 3 72 5 0.0 6 5 73 1 0.0 6 1 74 11 0.0 6 10 0 1 75 6 0.0 6 5 1 76 3 0.0 6 3 77 7 0.0 6 7 78 7 0.0 6 7 79 4 0.0 6 4 80 5 0.0 6 5 81 8 0.0 6 8 82 4 0.0 6 4 83 6 0.0 6 5 1 84 6 0.0 6 4 2 85 9 0.0 6 8 1 86 12 0.0 6 12 87 12 0.0 6 10 1 1 88 5 0.0 6 5 89 11 0.0 6 11 90 11 0.0 6 10 1 91 4 0.0 6 4 92 10 0.0 6 9 0 1 93 7 0.0 6 6 1 95 5 0.0 6 5 96 2 0.0 6 2 97 9 0.0 6 9 98 14 0.0 6 14 99 10 0.0 6 8 2 100 9 0.0 6 9 101 10 0.0 6 10 102 10 0.0 6 10 103 9 0.0 6 8 1 104 13 0.0 6 12 1 105 25 0.0 6 23 2 106 18 0.0 6 17 1 107 22 0.0 6 21 1 108 16 0.0 6 16 109 22 0.0 6 22 110 10 0.0 6 10 111 16 0.0 6 16 112 23 0.0 6 23 113 14 0.0 6 14 114 17 0.0 6 17 115 20 0.0 6 20 116 11 0.0 6 11 117 10 0.0 6 9 1 118 19 0.0 6 19 119 16 0.0 6 16 120 16 0.0 6 16 121 25 0.0 6 22 2 1 122 23 0.0 6 22 1 123 21 0.0 6 19 2 124 21 0.0 6 21 125 30 0.0 6 29 1 126 25 0.0 6 23 2 127 19 0.0 6 18 0 1 128 29 0.0 6 29 129 24 0.0 6 24 130 36 0.0 6 32 3 0 1 131 30 0.0 6 30 132 16 0.0 6 16 133 29 0.0 6 28 1 134 28 0.0 6 28 135 25 0.0 6 24 1 136 33 0.0 6 31 2 137 50 0.0 6 50 138 60 0.0 6 58 1 0 0 0 0 1 139 51 0.0 6 50 1 140 143 0.0 6 142 1 141 300 0.0 6 298 0 1 0 0 1 142 371 0.0 6 370 1 143 309 0.0 6 309 144 321 0.0 6 319 2 145 404 0.0 6 404 146 320 0.0 6 320 147 343 0.0 6 343 148 336 0.0 6 331 5 149 318 0.0 6 317 1 150 450 0.0 6 448 2 151 274 0.0 6 272 1 0 1 152 203 0.0 6 203 153 215 0.0 6 214 1 154 222 0.0 6 222 155 165 0.0 6 164 1 156 192 0.0 6 191 1 157 223 0.0 6 222 1 158 240 0.0 6 239 1 159 193 0.0 6 191 2 160 236 0.0 6 234 2 161 279 0.0 6 277 2 162 176 0.0 6 176 163 264 0.0 6 259 4 1 164 205 0.0 6 201 3 0 1 165 203 0.0 6 203 166 199 0.0 6 197 2 167 186 0.0 6 185 1 168 200 0.0 6 199 1 169 210 0.0 6 208 2 170 198 0.0 6 193 5 171 175 0.0 6 172 3 172 224 0.0 6 221 3 173 233 0.0 6 228 5 174 204 0.0 6 203 1 175 283 0.0 6 280 3 176 229 0.0 6 226 3 177 304 0.0 6 301 3 178 359 0.0 6 357 2 179 252 0.0 6 249 3 180 183 0.0 6 182 1 181 199 0.0 6 197 1 1 182 192 0.0 6 190 2 183 265 0.0 6 262 3 184 277 0.0 6 272 5 185 339 0.0 6 335 4 186 214 0.0 6 210 3 0 0 0 0 1 187 257 0.0 6 253 4 188 309 0.0 6 303 5 1 189 334 0.0 6 330 4 190 293 0.0 6 288 5 191 423 0.0 6 416 6 1 192 400 0.0 6 396 4 193 541 0.0 6 533 6 2 194 494 0.0 6 484 9 0 0 0 0 1 195 499 0.0 6 491 8 196 698 0.0 6 690 7 1 197 500 0.0 6 491 7 1 1 198 567 0.0 6 559 7 1 199 537 0.0 6 532 4 1 200 675 0.0 6 666 9 201 542 0.0 6 534 7 0 0 0 1 202 650 0.0 6 634 15 1 203 662 0.0 6 651 10 1 204 565 0.0 6 555 10 205 649 0.0 6 641 8 206 530 0.0 6 516 13 1 207 767 0.0 6 762 5 208 519 0.0 6 515 4 209 633 0.0 6 625 8 210 457 0.0 6 450 7 211 453 0.0 6 451 2 212 446 0.0 6 440 4 1 0 1 213 590 0.0 6 584 6 214 610 0.0 6 603 6 1 215 597 0.0 6 590 7 216 697 0.0 6 688 8 0 1 217 805 0.0 6 796 8 1 218 624 0.0 6 612 10 1 1 219 578 0.0 6 573 5 220 491 0.0 6 480 7 4 221 452 0.0 6 441 11 222 562 0.0 6 554 8 223 416 0.0 6 409 6 1 224 399 0.0 6 395 3 1 225 526 0.0 6 520 6 226 878 0.0 6 853 24 0 1 227 826 0.0 6 814 11 1 228 672 0.0 6 656 14 2 229 626 0.0 6 614 9 3 230 623 0.0 6 612 11 231 556 0.0 6 546 9 1 232 670 0.0 6 659 11 233 668 0.0 6 660 8 234 701 0.0 6 690 11 235 583 0.0 6 579 3 1 236 406 0.0 6 393 13 237 427 0.0 6 420 7 238 464 0.0 6 456 8 239 532 0.0 6 526 4 1 0 0 1 240 537 0.0 6 521 14 2 241 709 0.0 6 700 9 242 619 0.0 6 608 10 0 0 1 243 541 0.0 6 529 12 244 504 0.0 6 493 10 0 1 245 667 0.0 6 659 7 1 246 317 0.0 6 311 6 247 400 0.0 6 394 6 248 232 0.0 6 224 7 1 249 539 0.0 6 524 14 0 1 250 503 0.0 6 491 11 1 251 400 0.0 6 393 7 252 447 0.0 6 438 8 1 253 335 0.0 6 328 7 254 346 0.0 6 344 2 255 226 0.0 6 225 1 256 228 0.0 6 223 5 257 272 0.0 6 270 2 258 350 0.0 6 342 8 259 425 0.0 6 414 9 2 260 523 0.0 6 516 7 261 231 0.0 6 221 8 1 1 262 307 0.0 6 300 7 263 702 0.0 6 692 10 264 287 0.0 6 280 7 265 304 0.0 6 303 1 266 188 0.0 6 179 8 1 267 333 0.0 6 327 5 1 268 280 0.0 6 273 7 269 388 0.0 6 382 5 1 270 174 0.0 6 171 2 1 271 320 0.0 6 310 10 272 584 0.0 6 572 12 273 278 0.0 6 272 6 274 427 0.0 6 418 9 275 242 0.0 6 235 7 276 733 0.0 6 715 17 1 277 1264 0.0 6 1233 30 1 278 627 0.0 6 602 24 1 279 721 0.0 6 703 14 3 1 280 35832 0.0 6 35064 758 9 1 281 169 0.0 6 155 14 282 4 0.0 6 3 0 0 1 283 2 0.0 6 1 0 0 1 286 1 0.0 6 1 287 1 0.0 6 1 288 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 680 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 21 0.0 6 20 1 70 2 0.0 6 2 71 4 0.0 6 4 72 9 0.0 6 9 73 5 0.0 6 5 74 8 0.0 6 8 75 14 0.0 6 13 1 76 7 0.0 6 7 77 6 0.0 6 6 78 10 0.0 6 10 79 2 0.0 6 2 80 5 0.0 6 5 81 9 0.0 6 9 82 6 0.0 6 6 83 8 0.0 6 8 84 5 0.0 6 5 85 5 0.0 6 5 86 2 0.0 6 2 87 5 0.0 6 5 88 5 0.0 6 5 89 3 0.0 6 3 90 5 0.0 6 5 91 4 0.0 6 4 92 5 0.0 6 5 93 2 0.0 6 2 94 6 0.0 6 6 95 3 0.0 6 3 96 4 0.0 6 4 97 3 0.0 6 3 98 5 0.0 6 5 99 2 0.0 6 2 100 6 0.0 6 6 101 4 0.0 6 4 102 3 0.0 6 3 103 1 0.0 6 1 104 4 0.0 6 3 1 105 2 0.0 6 2 106 4 0.0 6 4 107 3 0.0 6 3 108 9 0.0 6 9 109 6 0.0 6 6 110 2 0.0 6 2 111 4 0.0 6 4 112 1 0.0 6 1 113 5 0.0 6 5 114 3 0.0 6 3 115 3 0.0 6 3 116 1 0.0 6 1 117 2 0.0 6 2 118 1 0.0 6 1 120 2 0.0 6 2 121 3 0.0 6 3 122 6 0.0 6 6 123 6 0.0 6 6 124 3 0.0 6 3 125 1 0.0 6 1 126 6 0.0 6 6 127 3 0.0 6 3 128 2 0.0 6 2 129 2 0.0 6 2 130 2 0.0 6 2 131 2 0.0 6 2 132 1 0.0 6 1 133 3 0.0 6 3 134 4 0.0 6 4 135 4 0.0 6 3 1 136 1 0.0 6 1 137 3 0.0 6 3 138 4 0.0 6 4 139 4 0.0 6 4 140 2 0.0 6 2 141 10 0.0 6 10 143 3 0.0 6 3 144 5 0.0 6 5 145 2 0.0 6 2 146 1 0.0 6 1 147 7 0.0 6 7 148 3 0.0 6 3 149 2 0.0 6 2 150 2 0.0 6 2 151 5 0.0 6 5 152 4 0.0 6 4 153 1 0.0 6 1 154 2 0.0 6 2 155 2 0.0 6 2 156 2 0.0 6 2 157 5 0.0 6 5 158 2 0.0 6 2 159 3 0.0 6 3 160 4 0.0 6 4 161 1 0.0 6 1 162 4 0.0 6 4 163 5 0.0 6 5 164 4 0.0 6 4 165 3 0.0 6 3 166 3 0.0 6 3 167 4 0.0 6 4 168 4 0.0 6 4 169 5 0.0 6 5 170 4 0.0 6 4 171 2 0.0 6 2 172 3 0.0 6 3 173 1 0.0 6 1 175 2 0.0 6 2 176 4 0.0 6 4 177 3 0.0 6 3 178 6 0.0 6 6 179 1 0.0 6 1 180 3 0.0 6 3 181 4 0.0 6 4 182 2 0.0 6 2 183 3 0.0 6 3 184 8 0.0 6 8 185 7 0.0 6 7 186 8 0.0 6 8 187 4 0.0 6 4 188 7 0.0 6 7 189 5 0.0 6 5 190 6 0.0 6 6 191 3 0.0 6 2 0 0 0 0 0 1 192 3 0.0 6 3 193 5 0.0 6 5 194 2 0.0 6 2 195 3 0.0 6 3 196 2 0.0 6 2 197 2 0.0 6 2 199 4 0.0 6 4 200 6 0.0 6 6 201 5 0.0 6 5 202 1 0.0 6 1 203 3 0.0 6 3 204 5 0.0 6 5 205 4 0.0 6 4 206 4 0.0 6 4 207 3 0.0 6 3 208 2 0.0 6 2 209 1 0.0 6 1 210 7 0.0 6 7 212 1 0.0 6 1 213 2 0.0 6 2 215 4 0.0 6 4 217 5 0.0 6 5 218 3 0.0 6 3 219 4 0.0 6 4 220 4 0.0 6 4 221 3 0.0 6 3 222 6 0.0 6 6 223 5 0.0 6 5 225 3 0.0 6 3 226 4 0.0 6 4 228 4 0.0 6 4 229 5 0.0 6 5 230 1 0.0 6 1 231 3 0.0 6 3 232 2 0.0 6 2 233 3 0.0 6 3 234 6 0.0 6 6 236 5 0.0 6 5 237 2 0.0 6 2 238 3 0.0 6 3 239 2 0.0 6 2 240 1 0.0 6 1 241 3 0.0 6 3 242 1 0.0 6 1 243 3 0.0 6 3 244 1 0.0 6 1 246 4 0.0 6 4 247 3 0.0 6 3 248 3 0.0 6 3 249 1 0.0 6 1 250 2 0.0 6 2 252 3 0.0 6 3 253 2 0.0 6 2 256 2 0.0 6 2 257 1 0.0 6 1 259 1 0.0 6 1 264 1 0.0 6 1 265 1 0.0 6 1 271 2 0.0 6 2 272 1 0.0 6 1 274 1 0.0 6 1 280 1 0.0 6 1 281 1 0.0 6 1 282 2 0.0 6 2 287 1 0.0 6 1 291 2 0.0 6 2 293 1 0.0 6 1 302 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 96310 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 23.7 1 1 7 1 5.9 2 0 1 8 1 1.5 2 1 9 1 0.4 2 1 10 1 0.1 3 1 11 7 0.0 3 6 1 12 4 0.0 3 4 13 6 0.0 3 3 3 14 8 0.0 4 7 0 1 15 1 0.0 4 1 16 8 0.0 4 6 2 17 24 0.0 5 22 1 1 18 5 0.0 5 5 19 43 0.0 5 37 5 1 20 127 0.0 6 123 3 1 21 84 0.0 6 81 3 22 58 0.0 6 54 4 23 38 0.0 6 35 3 24 27 0.0 6 24 3 25 17 0.0 6 16 1 26 29 0.0 6 26 3 27 21 0.0 6 20 1 28 14 0.0 6 14 29 13 0.0 6 12 1 30 15 0.0 6 14 0 1 31 15 0.0 6 13 2 32 15 0.0 6 14 0 0 0 0 0 1 33 18 0.0 6 16 1 1 34 18 0.0 6 14 3 1 35 12 0.0 6 10 2 36 8 0.0 6 7 1 37 15 0.0 6 14 1 38 14 0.0 6 14 39 17 0.0 6 13 3 1 40 8 0.0 6 5 2 0 0 1 41 13 0.0 6 10 2 0 0 1 42 16 0.0 6 15 1 43 7 0.0 6 6 1 44 18 0.0 6 17 1 45 12 0.0 6 10 2 46 18 0.0 6 18 47 18 0.0 6 17 1 48 17 0.0 6 15 2 49 24 0.0 6 21 2 1 50 17 0.0 6 16 1 51 26 0.0 6 22 4 52 22 0.0 6 18 4 53 22 0.0 6 21 1 54 22 0.0 6 20 2 55 27 0.0 6 25 0 1 1 56 47 0.0 6 44 3 57 28 0.0 6 25 1 2 58 22 0.0 6 19 3 59 26 0.0 6 22 4 60 21 0.0 6 15 6 61 23 0.0 6 22 1 62 22 0.0 6 16 5 0 0 0 0 1 63 20 0.0 6 17 2 1 64 17 0.0 6 16 1 65 13 0.0 6 13 66 24 0.0 6 22 1 0 0 1 67 34 0.0 6 28 5 1 68 21 0.0 6 19 1 1 69 58 0.0 6 56 0 2 70 181 0.0 6 169 11 0 0 1 71 62 0.0 6 59 1 1 0 1 72 100 0.0 6 94 5 1 73 46 0.0 6 42 4 74 73 0.0 6 70 2 0 1 75 28 0.0 6 24 3 0 0 1 76 29 0.0 6 27 2 77 47 0.0 6 47 78 41 0.0 6 37 4 79 38 0.0 6 37 1 80 45 0.0 6 44 1 81 42 0.0 6 39 2 0 0 1 82 30 0.0 6 28 1 1 83 46 0.0 6 44 2 84 44 0.0 6 41 2 1 85 46 0.0 6 44 2 86 63 0.0 6 60 2 0 0 1 87 38 0.0 6 35 3 88 42 0.0 6 40 2 89 50 0.0 6 46 2 2 90 77 0.0 6 68 9 91 58 0.0 6 56 1 1 92 47 0.0 6 44 2 0 0 1 93 65 0.0 6 60 4 0 0 1 94 110 0.0 6 98 10 2 95 57 0.0 6 54 2 0 0 0 1 96 79 0.0 6 74 5 97 62 0.0 6 60 2 98 61 0.0 6 57 2 0 0 2 99 97 0.0 6 92 5 100 75 0.0 6 71 4 101 81 0.0 6 77 4 102 100 0.0 6 95 4 0 0 1 103 127 0.0 6 121 4 1 0 1 104 120 0.0 6 113 7 105 123 0.0 6 116 6 0 0 1 106 81 0.0 6 77 4 107 173 0.0 6 163 8 1 1 108 88 0.0 6 84 3 0 0 1 109 85 0.0 6 82 2 1 110 89 0.0 6 85 4 111 85 0.0 6 81 2 1 0 1 112 110 0.0 6 109 1 113 122 0.0 6 115 5 1 0 1 114 109 0.0 6 105 4 115 135 0.0 6 131 3 1 116 125 0.0 6 119 6 117 84 0.0 6 83 1 118 130 0.0 6 121 8 1 119 152 0.0 6 148 3 0 1 120 160 0.0 6 157 3 121 152 0.0 6 145 7 122 184 0.0 6 178 6 123 167 0.0 6 161 5 0 0 1 124 183 0.0 6 179 3 1 125 154 0.0 6 144 9 1 126 140 0.0 6 137 0 2 0 0 0 1 127 192 0.0 6 189 2 0 0 1 128 198 0.0 6 193 4 0 0 1 129 206 0.0 6 196 6 3 1 130 231 0.0 6 226 4 0 0 1 131 240 0.0 6 232 6 1 0 0 1 132 270 0.0 6 263 7 133 262 0.0 6 260 1 1 134 281 0.0 6 276 5 135 477 0.0 6 460 17 136 398 0.0 6 389 7 1 0 1 137 510 0.0 6 492 16 0 0 2 138 433 0.0 6 422 9 1 0 1 139 490 0.0 6 475 12 1 1 1 140 627 0.0 6 616 11 141 709 0.0 6 702 6 0 0 0 1 142 790 0.0 6 775 14 1 143 789 0.0 6 772 15 0 0 1 0 1 144 905 0.0 6 887 16 1 1 145 761 0.0 6 750 9 0 1 1 146 709 0.0 6 701 6 0 1 0 1 147 670 0.0 6 666 3 1 148 698 0.0 6 680 15 3 149 918 0.0 6 897 19 1 0 0 0 1 150 949 0.0 6 919 25 3 1 0 1 151 764 0.0 6 749 13 1 1 152 1029 0.0 6 1002 20 3 1 2 1 153 1187 0.0 6 1156 26 0 1 2 1 1 154 1041 0.0 6 1019 22 155 1231 0.0 6 1203 24 2 1 1 156 1153 0.0 6 1139 13 0 0 1 157 1216 0.0 6 1181 32 0 1 1 0 1 158 1428 0.0 6 1386 39 1 0 1 1 159 1052 0.0 6 1026 24 1 1 160 1360 0.0 6 1332 26 2 161 883 0.0 6 870 11 0 1 1 162 905 0.0 6 888 14 2 0 1 163 950 0.0 6 928 20 0 1 1 164 820 0.0 6 801 15 1 0 3 165 993 0.0 6 978 14 1 166 1060 0.0 6 1038 21 1 167 745 0.0 6 731 14 168 1133 0.0 6 1105 26 0 0 2 169 697 0.0 6 686 10 1 170 693 0.0 6 681 12 171 1075 0.0 6 1055 18 1 1 172 666 0.0 6 648 16 2 173 708 0.0 6 694 8 5 0 0 1 174 1004 0.0 6 976 27 0 0 0 1 175 1418 0.0 6 1393 23 2 176 972 0.0 6 942 27 2 0 1 177 1503 0.0 6 1465 34 1 1 2 178 931 0.0 6 901 27 1 0 0 0 2 179 1224 0.0 6 1192 28 4 180 1039 0.0 6 1009 27 1 2 181 1483 0.0 6 1446 34 0 1 2 182 600 0.0 6 580 17 1 2 183 920 0.0 6 901 18 0 0 0 0 1 184 459 0.0 6 449 10 185 503 0.0 6 482 18 3 186 1378 0.0 6 1343 30 2 0 3 187 1805 0.0 6 1752 51 1 0 1 188 1740 0.0 6 1679 59 2 189 1510 0.0 6 1468 40 0 0 0 1 1 190 1967 0.0 6 1922 43 1 0 1 191 1540 0.0 6 1498 37 1 1 1 0 2 192 1351 0.0 6 1315 31 2 1 2 193 1006 0.0 6 979 25 0 1 1 194 1635 0.0 6 1598 36 1 195 1218 0.0 6 1179 37 1 1 196 3054 0.0 6 2966 84 2 0 2 197 1189 0.0 6 1144 41 3 0 1 198 1889 0.0 6 1815 71 2 0 1 199 3771 0.0 6 3663 102 3 0 3 200 1109 0.0 6 1070 37 1 0 1 201 1904 0.0 6 1865 38 0 0 0 1 202 946 0.0 6 920 23 1 1 1 203 798 0.0 6 774 23 1 204 1399 0.0 6 1374 25 205 481 0.0 6 465 14 1 0 1 206 581 0.0 6 559 21 1 207 688 0.0 6 668 18 1 0 1 208 666 0.0 6 660 6 209 467 0.0 6 455 11 0 1 210 365 0.0 6 355 10 211 375 0.0 6 365 10 212 494 0.0 6 481 12 1 213 209 0.0 6 201 8 214 846 0.0 6 828 17 1 215 103 0.0 6 98 5 216 301 0.0 6 292 9 217 489 0.0 6 474 14 0 0 1 218 227 0.0 6 220 6 0 1 219 266 0.0 6 258 7 1 220 251 0.0 6 247 4 221 496 0.0 6 482 14 222 122 0.0 6 118 4 223 275 0.0 6 266 8 1 224 198 0.0 6 192 6 225 359 0.0 6 350 9 226 340 0.0 6 329 11 227 213 0.0 6 203 9 0 0 1 228 120 0.0 6 118 1 0 0 1 229 234 0.0 6 230 4 230 124 0.0 6 122 2 231 122 0.0 6 117 4 1 232 182 0.0 6 179 3 233 209 0.0 6 200 9 234 81 0.0 6 79 2 235 112 0.0 6 110 2 236 36 0.0 6 33 3 237 59 0.0 6 54 5 238 19 0.0 6 19 239 31 0.0 6 30 1 240 68 0.0 6 66 2 241 105 0.0 6 103 2 242 163 0.0 6 163 243 87 0.0 6 85 1 1 244 222 0.0 6 216 6 245 23 0.0 6 22 1 246 28 0.0 6 27 1 247 14 0.0 6 14 248 22 0.0 6 21 1 249 21 0.0 6 21 250 19 0.0 6 19 251 27 0.0 6 27 252 11 0.0 6 11 253 5 0.0 6 5 254 14 0.0 6 14 255 4 0.0 6 4 256 4 0.0 6 4 257 4 0.0 6 4 258 4 0.0 6 4 259 2 0.0 6 2 279 1 0.0 6 1 280 2 0.0 6 2 281 9 0.0 6 9 282 54 0.0 6 51 2 1 283 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 565 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 49 2 0.0 6 2 62 1 0.0 6 1 64 2 0.0 6 2 69 2 0.0 6 2 70 8 0.0 6 8 71 8 0.0 6 8 72 6 0.0 6 6 73 8 0.0 6 8 74 17 0.0 6 17 75 6 0.0 6 6 76 6 0.0 6 6 77 6 0.0 6 6 78 6 0.0 6 6 79 13 0.0 6 12 0 1 80 10 0.0 6 10 81 4 0.0 6 4 82 7 0.0 6 7 83 7 0.0 6 7 84 3 0.0 6 3 85 4 0.0 6 4 86 6 0.0 6 6 87 5 0.0 6 5 88 6 0.0 6 6 89 6 0.0 6 6 90 2 0.0 6 2 91 5 0.0 6 5 92 6 0.0 6 6 93 5 0.0 6 4 0 0 0 1 94 5 0.0 6 5 95 5 0.0 6 5 96 7 0.0 6 7 97 1 0.0 6 1 98 2 0.0 6 2 99 6 0.0 6 6 100 4 0.0 6 4 101 5 0.0 6 5 102 2 0.0 6 2 103 5 0.0 6 5 104 2 0.0 6 2 105 5 0.0 6 5 106 6 0.0 6 6 107 11 0.0 6 11 108 2 0.0 6 2 109 4 0.0 6 4 110 4 0.0 6 4 111 2 0.0 6 2 112 3 0.0 6 3 113 4 0.0 6 4 114 3 0.0 6 3 116 2 0.0 6 2 117 4 0.0 6 4 118 3 0.0 6 3 119 2 0.0 6 2 120 5 0.0 6 5 121 4 0.0 6 4 122 5 0.0 6 5 123 1 0.0 6 1 124 7 0.0 6 6 0 0 0 1 125 8 0.0 6 8 126 4 0.0 6 4 127 1 0.0 6 1 128 4 0.0 6 4 129 3 0.0 6 3 130 3 0.0 6 3 131 4 0.0 6 4 132 1 0.0 6 1 133 3 0.0 6 3 134 13 0.0 6 13 135 3 0.0 6 3 136 2 0.0 6 2 137 4 0.0 6 4 138 6 0.0 6 6 139 5 0.0 6 5 140 4 0.0 6 4 141 4 0.0 6 4 142 5 0.0 6 5 143 2 0.0 6 2 144 5 0.0 6 5 145 2 0.0 6 2 146 7 0.0 6 7 147 1 0.0 6 1 148 6 0.0 6 6 149 5 0.0 6 5 150 4 0.0 6 4 151 1 0.0 6 1 152 1 0.0 6 1 153 4 0.0 6 4 155 2 0.0 6 2 156 5 0.0 6 5 157 3 0.0 6 3 158 1 0.0 6 1 159 3 0.0 6 3 160 3 0.0 6 3 161 5 0.0 6 5 162 4 0.0 6 3 1 163 2 0.0 6 2 164 9 0.0 6 9 165 5 0.0 6 5 167 4 0.0 6 4 168 2 0.0 6 2 169 2 0.0 6 2 170 7 0.0 6 7 171 3 0.0 6 3 172 7 0.0 6 7 173 5 0.0 6 4 1 174 1 0.0 6 1 175 2 0.0 6 2 176 5 0.0 6 5 177 5 0.0 6 5 178 6 0.0 6 6 179 2 0.0 6 2 180 6 0.0 6 6 181 5 0.0 6 5 182 2 0.0 6 2 184 5 0.0 6 5 185 2 0.0 6 2 186 2 0.0 6 2 187 2 0.0 6 2 188 1 0.0 6 1 189 3 0.0 6 3 190 4 0.0 6 4 191 4 0.0 6 4 192 2 0.0 6 2 193 1 0.0 6 1 194 2 0.0 6 2 195 1 0.0 6 1 196 2 0.0 6 2 197 3 0.0 6 3 198 1 0.0 6 1 200 1 0.0 6 1 201 2 0.0 6 2 203 2 0.0 6 2 207 2 0.0 6 2 208 1 0.0 6 1 210 1 0.0 6 1 211 2 0.0 6 2 214 1 0.0 6 1 215 1 0.0 6 1 217 1 0.0 6 1 219 1 0.0 6 1 222 1 0.0 6 1 224 1 0.0 6 1 227 1 0.0 6 1 229 1 0.0 6 1 231 1 0.0 6 1 242 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_15_6_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_15_233_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_15_6_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_15_233_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.78 s (53 µs/read; 1.12 M reads/minute). === Summary === Total read pairs processed: 33,287 Read 1 with adapter: 33,283 (100.0%) Read 2 with adapter: 33,268 (99.9%) == Read fate breakdown == Pairs that were too short: 24 (0.1%) Pairs discarded as untrimmed: 23 (0.1%) Pairs written (passing filters): 33,240 (99.9%) Total basepairs processed: 14,486,489 bp Read 1: 8,115,596 bp Read 2: 6,370,893 bp Total written (filtered): 2,156,795 bp (14.9%) Read 1: 1,074,389 bp Read 2: 1,082,406 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 32368 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 520.1 0 1 4 2 130.0 1 1 1 5 1 32.5 1 1 6 2 8.1 1 1 0 1 7 1 2.0 2 0 0 1 8 1 0.5 2 1 9 2 0.1 2 2 14 1 0.0 4 1 16 1 0.0 4 0 1 17 2 0.0 5 2 19 3 0.0 5 2 1 20 69 0.0 6 67 2 21 41 0.0 6 39 2 22 23 0.0 6 22 1 23 15 0.0 6 15 24 13 0.0 6 13 25 9 0.0 6 9 26 7 0.0 6 7 27 3 0.0 6 3 28 6 0.0 6 6 29 5 0.0 6 5 30 3 0.0 6 3 31 8 0.0 6 8 32 3 0.0 6 3 33 2 0.0 6 2 34 2 0.0 6 2 35 2 0.0 6 2 36 5 0.0 6 5 37 3 0.0 6 3 38 5 0.0 6 5 39 3 0.0 6 3 40 1 0.0 6 1 41 5 0.0 6 5 42 1 0.0 6 1 43 3 0.0 6 3 44 3 0.0 6 3 45 3 0.0 6 3 46 6 0.0 6 6 47 5 0.0 6 5 48 3 0.0 6 3 49 5 0.0 6 5 50 3 0.0 6 3 51 4 0.0 6 3 1 52 4 0.0 6 4 53 1 0.0 6 1 54 3 0.0 6 3 55 2 0.0 6 2 56 3 0.0 6 3 57 2 0.0 6 2 58 1 0.0 6 1 59 1 0.0 6 1 60 1 0.0 6 1 61 2 0.0 6 2 62 2 0.0 6 2 63 2 0.0 6 2 64 2 0.0 6 2 65 2 0.0 6 2 66 5 0.0 6 5 67 4 0.0 6 3 1 68 2 0.0 6 2 69 7 0.0 6 7 70 4 0.0 6 4 71 5 0.0 6 5 72 2 0.0 6 2 73 2 0.0 6 2 74 3 0.0 6 3 75 2 0.0 6 2 76 4 0.0 6 4 77 4 0.0 6 4 78 3 0.0 6 3 79 3 0.0 6 3 80 5 0.0 6 5 81 3 0.0 6 3 82 4 0.0 6 3 1 83 6 0.0 6 6 84 6 0.0 6 6 85 7 0.0 6 7 86 3 0.0 6 2 1 87 2 0.0 6 2 88 5 0.0 6 5 89 6 0.0 6 6 90 7 0.0 6 6 1 91 5 0.0 6 4 1 92 3 0.0 6 2 1 93 2 0.0 6 2 94 3 0.0 6 3 95 8 0.0 6 8 96 4 0.0 6 4 97 3 0.0 6 3 98 8 0.0 6 8 99 3 0.0 6 3 100 5 0.0 6 5 101 5 0.0 6 5 102 5 0.0 6 5 103 3 0.0 6 3 104 10 0.0 6 10 105 3 0.0 6 3 106 8 0.0 6 8 107 2 0.0 6 2 108 6 0.0 6 6 109 2 0.0 6 2 110 4 0.0 6 4 111 7 0.0 6 7 112 2 0.0 6 1 1 113 6 0.0 6 5 1 114 6 0.0 6 6 115 8 0.0 6 8 116 9 0.0 6 9 117 10 0.0 6 10 118 8 0.0 6 8 119 8 0.0 6 7 1 120 12 0.0 6 12 121 10 0.0 6 7 2 0 1 122 7 0.0 6 7 123 10 0.0 6 9 1 124 11 0.0 6 10 1 125 9 0.0 6 9 126 10 0.0 6 9 1 127 11 0.0 6 11 128 9 0.0 6 8 1 129 17 0.0 6 16 1 130 7 0.0 6 6 0 1 131 19 0.0 6 19 132 7 0.0 6 7 133 8 0.0 6 8 134 17 0.0 6 17 135 13 0.0 6 12 1 136 9 0.0 6 8 1 137 11 0.0 6 11 138 18 0.0 6 16 1 1 139 17 0.0 6 17 140 14 0.0 6 13 1 141 19 0.0 6 17 1 0 0 0 1 142 18 0.0 6 18 143 51 0.0 6 51 144 82 0.0 6 81 1 145 107 0.0 6 105 1 0 0 0 0 1 146 74 0.0 6 74 147 109 0.0 6 109 148 156 0.0 6 155 1 149 64 0.0 6 64 150 67 0.0 6 66 1 151 65 0.0 6 65 152 115 0.0 6 115 153 135 0.0 6 135 154 97 0.0 6 96 1 155 120 0.0 6 117 3 156 48 0.0 6 47 1 157 76 0.0 6 76 158 88 0.0 6 87 1 159 93 0.0 6 93 160 68 0.0 6 67 1 161 95 0.0 6 94 1 162 121 0.0 6 121 163 111 0.0 6 111 164 170 0.0 6 170 165 167 0.0 6 165 1 1 166 110 0.0 6 110 167 93 0.0 6 92 1 168 85 0.0 6 85 169 92 0.0 6 91 1 170 125 0.0 6 122 3 171 204 0.0 6 203 1 172 120 0.0 6 118 1 0 0 0 0 1 173 101 0.0 6 101 174 104 0.0 6 103 1 175 124 0.0 6 124 176 138 0.0 6 138 177 141 0.0 6 139 2 178 137 0.0 6 135 2 179 103 0.0 6 102 1 180 176 0.0 6 175 1 181 314 0.0 6 313 1 182 167 0.0 6 166 1 183 130 0.0 6 128 2 184 127 0.0 6 127 185 108 0.0 6 106 2 186 121 0.0 6 120 1 187 215 0.0 6 215 188 315 0.0 6 311 4 189 212 0.0 6 211 1 190 253 0.0 6 252 0 1 191 234 0.0 6 233 1 192 291 0.0 6 291 193 682 0.0 6 680 1 0 0 1 194 638 0.0 6 634 3 0 0 0 1 195 243 0.0 6 240 1 0 1 0 1 196 265 0.0 6 260 4 0 0 0 0 1 197 249 0.0 6 249 198 453 0.0 6 448 5 199 408 0.0 6 403 4 1 200 323 0.0 6 322 0 0 0 1 201 229 0.0 6 229 202 364 0.0 6 359 5 203 236 0.0 6 236 204 290 0.0 6 286 4 205 558 0.0 6 558 206 271 0.0 6 270 1 207 406 0.0 6 397 7 1 0 1 208 293 0.0 6 291 2 209 425 0.0 6 422 3 210 311 0.0 6 310 1 211 339 0.0 6 336 2 0 0 0 1 212 390 0.0 6 387 2 0 0 0 0 1 213 392 0.0 6 389 1 0 0 0 1 1 214 366 0.0 6 360 6 215 465 0.0 6 461 4 216 534 0.0 6 532 2 217 491 0.0 6 488 2 0 1 218 467 0.0 6 462 5 219 440 0.0 6 439 1 220 425 0.0 6 421 4 221 361 0.0 6 360 0 0 0 0 0 1 222 367 0.0 6 366 1 223 408 0.0 6 406 2 224 264 0.0 6 261 2 0 0 0 1 225 355 0.0 6 351 2 0 0 0 2 226 408 0.0 6 407 0 0 0 0 0 1 227 440 0.0 6 434 5 0 0 0 0 1 228 350 0.0 6 349 1 229 436 0.0 6 432 3 0 1 230 294 0.0 6 289 5 231 325 0.0 6 324 1 232 277 0.0 6 274 2 1 233 332 0.0 6 330 1 0 0 1 234 347 0.0 6 346 1 235 391 0.0 6 389 1 1 236 354 0.0 6 354 237 331 0.0 6 329 2 238 359 0.0 6 356 2 0 0 0 1 239 246 0.0 6 243 3 240 251 0.0 6 249 2 241 340 0.0 6 338 2 242 293 0.0 6 290 1 1 0 1 243 256 0.0 6 256 244 290 0.0 6 289 0 1 245 203 0.0 6 202 1 246 221 0.0 6 217 3 0 0 0 1 247 204 0.0 6 203 1 248 189 0.0 6 188 1 249 159 0.0 6 159 250 232 0.0 6 230 1 1 251 213 0.0 6 212 1 252 192 0.0 6 192 253 168 0.0 6 168 254 167 0.0 6 167 255 175 0.0 6 175 256 138 0.0 6 137 1 257 181 0.0 6 180 0 0 0 0 1 258 142 0.0 6 142 259 184 0.0 6 183 1 260 245 0.0 6 244 1 261 108 0.0 6 106 2 262 88 0.0 6 88 263 81 0.0 6 81 264 103 0.0 6 101 2 265 93 0.0 6 93 266 86 0.0 6 86 267 96 0.0 6 95 1 268 102 0.0 6 101 1 269 85 0.0 6 85 270 82 0.0 6 82 271 83 0.0 6 82 1 272 62 0.0 6 61 1 273 75 0.0 6 74 1 274 92 0.0 6 91 1 275 72 0.0 6 72 276 100 0.0 6 100 277 126 0.0 6 126 278 154 0.0 6 153 1 279 550 0.0 6 547 3 280 604 0.0 6 599 5 281 5 0.0 6 5 282 2 0.0 6 2 284 1 0.0 6 1 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 915 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 57 1 0.0 6 1 62 1 0.0 6 1 70 2 0.0 6 2 71 3 0.0 6 3 72 4 0.0 6 4 73 3 0.0 6 3 74 2 0.0 6 2 75 2 0.0 6 2 76 5 0.0 6 5 77 1 0.0 6 1 79 1 0.0 6 1 80 1 0.0 6 1 81 3 0.0 6 3 82 6 0.0 6 6 83 3 0.0 6 3 84 1 0.0 6 1 85 2 0.0 6 2 87 2 0.0 6 2 89 1 0.0 6 1 90 2 0.0 6 2 91 4 0.0 6 4 92 3 0.0 6 3 93 7 0.0 6 7 94 2 0.0 6 2 95 1 0.0 6 1 96 1 0.0 6 1 97 2 0.0 6 2 98 3 0.0 6 2 0 0 0 0 0 1 99 6 0.0 6 6 100 1 0.0 6 1 101 1 0.0 6 1 102 1 0.0 6 1 103 3 0.0 6 3 104 3 0.0 6 3 105 2 0.0 6 2 106 4 0.0 6 4 107 3 0.0 6 3 108 6 0.0 6 6 109 8 0.0 6 8 110 5 0.0 6 5 111 2 0.0 6 2 112 2 0.0 6 2 113 4 0.0 6 4 114 1 0.0 6 1 115 7 0.0 6 7 116 8 0.0 6 8 117 2 0.0 6 2 118 8 0.0 6 8 119 3 0.0 6 3 120 15 0.0 6 14 1 121 21 0.0 6 21 122 27 0.0 6 27 123 3 0.0 6 3 124 3 0.0 6 3 125 4 0.0 6 4 126 6 0.0 6 6 127 7 0.0 6 7 128 5 0.0 6 5 129 2 0.0 6 2 130 2 0.0 6 2 131 2 0.0 6 2 132 6 0.0 6 6 133 20 0.0 6 20 134 5 0.0 6 5 135 3 0.0 6 3 136 3 0.0 6 3 137 6 0.0 6 6 138 3 0.0 6 3 139 5 0.0 6 5 140 1 0.0 6 1 141 5 0.0 6 5 142 5 0.0 6 5 143 6 0.0 6 6 144 3 0.0 6 3 145 5 0.0 6 5 146 5 0.0 6 5 147 3 0.0 6 3 148 7 0.0 6 7 149 20 0.0 6 20 150 15 0.0 6 15 151 7 0.0 6 7 152 6 0.0 6 6 153 5 0.0 6 5 154 3 0.0 6 3 155 1 0.0 6 1 156 5 0.0 6 5 157 9 0.0 6 9 158 2 0.0 6 2 159 7 0.0 6 7 160 1 0.0 6 1 161 6 0.0 6 6 162 6 0.0 6 6 163 8 0.0 6 8 164 11 0.0 6 11 165 8 0.0 6 8 166 17 0.0 6 17 167 7 0.0 6 6 1 168 7 0.0 6 7 169 7 0.0 6 7 170 7 0.0 6 7 171 8 0.0 6 8 172 4 0.0 6 4 173 8 0.0 6 8 174 4 0.0 6 4 175 10 0.0 6 10 176 6 0.0 6 6 177 3 0.0 6 3 178 16 0.0 6 16 179 14 0.0 6 14 180 3 0.0 6 3 181 7 0.0 6 7 182 1 0.0 6 1 183 6 0.0 6 6 184 19 0.0 6 19 185 2 0.0 6 1 1 186 2 0.0 6 2 187 8 0.0 6 8 188 15 0.0 6 15 189 8 0.0 6 8 190 13 0.0 6 13 191 4 0.0 6 4 192 3 0.0 6 3 194 2 0.0 6 2 195 1 0.0 6 1 196 9 0.0 6 9 197 11 0.0 6 11 198 6 0.0 6 6 199 2 0.0 6 2 200 4 0.0 6 4 201 2 0.0 6 2 202 12 0.0 6 12 203 6 0.0 6 6 204 4 0.0 6 4 205 5 0.0 6 5 206 3 0.0 6 3 207 2 0.0 6 2 208 2 0.0 6 2 209 1 0.0 6 1 210 3 0.0 6 3 211 4 0.0 6 4 212 4 0.0 6 4 213 1 0.0 6 1 214 1 0.0 6 1 215 3 0.0 6 3 216 3 0.0 6 3 217 6 0.0 6 6 218 8 0.0 6 8 219 2 0.0 6 2 220 11 0.0 6 11 221 22 0.0 6 22 222 3 0.0 6 3 223 3 0.0 6 3 224 2 0.0 6 2 225 2 0.0 6 2 226 4 0.0 6 4 227 6 0.0 6 6 228 4 0.0 6 4 229 7 0.0 6 7 230 1 0.0 6 1 231 5 0.0 6 5 232 5 0.0 6 5 233 1 0.0 6 1 234 10 0.0 6 10 235 3 0.0 6 3 236 1 0.0 6 1 237 3 0.0 6 3 239 2 0.0 6 2 240 3 0.0 6 3 241 1 0.0 6 1 242 4 0.0 6 4 243 1 0.0 6 1 244 1 0.0 6 1 245 1 0.0 6 1 247 3 0.0 6 3 248 1 0.0 6 1 249 2 0.0 6 2 250 2 0.0 6 2 251 4 0.0 6 4 253 1 0.0 6 1 256 2 0.0 6 2 258 1 0.0 6 1 262 1 0.0 6 1 263 1 0.0 6 1 265 2 0.0 6 2 266 1 0.0 6 1 267 1 0.0 6 1 268 1 0.0 6 1 269 1 0.0 6 1 270 1 0.0 6 1 272 1 0.0 6 1 274 1 0.0 6 1 278 2 0.0 6 2 279 1 0.0 6 1 280 1 0.0 6 1 283 1 0.0 6 1 286 1 0.0 6 1 293 1 0.0 6 1 301 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 32457 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 2.0 2 1 8 1 0.5 2 0 1 11 1 0.0 3 1 12 1 0.0 3 0 0 0 1 13 4 0.0 3 4 14 6 0.0 4 6 15 3 0.0 4 2 1 16 5 0.0 4 3 2 17 18 0.0 5 16 2 18 8 0.0 5 8 19 14 0.0 5 13 1 20 66 0.0 6 66 21 22 0.0 6 21 1 22 15 0.0 6 12 3 23 15 0.0 6 14 1 24 12 0.0 6 11 1 25 6 0.0 6 6 26 8 0.0 6 7 1 27 7 0.0 6 6 1 28 10 0.0 6 8 1 0 1 29 11 0.0 6 9 2 30 7 0.0 6 5 2 31 5 0.0 6 5 32 8 0.0 6 7 0 0 0 0 0 1 33 8 0.0 6 8 34 8 0.0 6 6 2 35 4 0.0 6 4 36 13 0.0 6 13 37 4 0.0 6 4 38 16 0.0 6 15 0 0 1 39 7 0.0 6 6 1 40 7 0.0 6 6 1 41 10 0.0 6 10 42 7 0.0 6 7 43 8 0.0 6 7 1 44 6 0.0 6 5 0 0 0 0 1 45 6 0.0 6 6 46 3 0.0 6 3 47 3 0.0 6 3 48 17 0.0 6 16 1 49 8 0.0 6 8 50 2 0.0 6 2 51 13 0.0 6 13 52 13 0.0 6 12 1 53 8 0.0 6 8 54 11 0.0 6 9 2 55 11 0.0 6 10 1 56 12 0.0 6 9 3 57 10 0.0 6 9 1 58 11 0.0 6 10 0 0 1 59 12 0.0 6 11 1 60 7 0.0 6 6 1 61 17 0.0 6 17 62 12 0.0 6 12 63 14 0.0 6 13 1 64 7 0.0 6 7 65 16 0.0 6 14 2 66 13 0.0 6 12 1 67 62 0.0 6 60 1 0 0 0 1 68 29 0.0 6 22 7 69 29 0.0 6 27 1 0 1 70 26 0.0 6 25 1 71 24 0.0 6 24 72 17 0.0 6 15 2 73 15 0.0 6 13 1 1 74 20 0.0 6 20 75 20 0.0 6 20 76 21 0.0 6 21 77 33 0.0 6 29 3 0 1 78 18 0.0 6 17 1 79 25 0.0 6 22 2 0 0 1 80 26 0.0 6 24 1 0 1 81 24 0.0 6 24 82 21 0.0 6 20 0 0 0 0 0 1 83 35 0.0 6 34 0 0 1 84 18 0.0 6 16 1 0 0 0 1 85 19 0.0 6 17 1 0 1 86 19 0.0 6 18 1 87 18 0.0 6 17 1 88 16 0.0 6 15 1 89 22 0.0 6 19 1 1 1 90 25 0.0 6 25 91 43 0.0 6 42 1 92 22 0.0 6 21 1 93 22 0.0 6 20 1 1 94 32 0.0 6 32 95 32 0.0 6 32 96 37 0.0 6 33 2 1 1 97 26 0.0 6 22 2 0 1 1 98 32 0.0 6 32 99 23 0.0 6 19 3 1 100 38 0.0 6 36 2 101 41 0.0 6 40 1 102 46 0.0 6 44 2 103 70 0.0 6 65 5 104 98 0.0 6 95 3 105 45 0.0 6 42 3 106 49 0.0 6 46 0 1 0 0 0 2 107 66 0.0 6 66 108 64 0.0 6 60 3 1 109 55 0.0 6 52 2 0 1 110 62 0.0 6 59 2 0 1 111 58 0.0 6 53 4 0 1 112 72 0.0 6 69 3 113 59 0.0 6 56 3 114 76 0.0 6 72 3 0 0 1 115 81 0.0 6 79 2 116 83 0.0 6 83 117 74 0.0 6 71 2 0 0 0 1 118 77 0.0 6 76 1 119 88 0.0 6 84 4 120 79 0.0 6 78 1 121 68 0.0 6 65 2 1 122 97 0.0 6 96 0 0 1 123 86 0.0 6 83 3 124 101 0.0 6 94 6 0 1 125 142 0.0 6 137 5 126 101 0.0 6 99 1 1 127 113 0.0 6 107 4 1 1 128 152 0.0 6 145 5 1 0 0 1 129 150 0.0 6 148 1 1 130 146 0.0 6 143 2 0 0 0 0 1 131 173 0.0 6 169 3 1 132 158 0.0 6 155 2 0 0 0 1 133 211 0.0 6 204 6 1 134 218 0.0 6 208 9 0 1 135 201 0.0 6 192 5 0 1 0 1 2 136 211 0.0 6 205 5 0 0 0 1 137 173 0.0 6 167 5 0 1 138 217 0.0 6 213 4 139 219 0.0 6 212 6 0 0 0 0 1 140 303 0.0 6 296 5 0 0 0 0 2 141 319 0.0 6 311 5 3 142 364 0.0 6 355 9 143 332 0.0 6 326 6 144 383 0.0 6 378 3 1 0 0 1 145 396 0.0 6 389 7 146 409 0.0 6 394 11 0 2 0 1 1 147 399 0.0 6 392 6 0 0 1 148 484 0.0 6 470 12 0 0 0 1 1 149 494 0.0 6 480 12 1 0 0 1 150 722 0.0 6 706 15 0 1 151 525 0.0 6 513 7 2 2 1 152 612 0.0 6 601 7 1 2 0 0 1 153 562 0.0 6 548 12 1 0 1 154 507 0.0 6 499 7 0 0 0 0 1 155 525 0.0 6 516 8 0 0 0 1 156 489 0.0 6 486 3 157 531 0.0 6 521 9 0 0 1 158 508 0.0 6 499 8 0 1 159 531 0.0 6 516 12 2 0 1 160 554 0.0 6 549 5 161 462 0.0 6 454 7 0 0 1 162 421 0.0 6 414 5 2 163 442 0.0 6 435 6 0 0 0 1 164 408 0.0 6 400 8 165 392 0.0 6 387 5 166 526 0.0 6 514 7 0 1 1 2 1 167 499 0.0 6 490 8 0 0 1 168 503 0.0 6 496 5 1 0 0 0 1 169 487 0.0 6 480 6 0 0 1 170 406 0.0 6 394 9 0 0 1 1 1 171 500 0.0 6 489 8 2 0 0 1 172 513 0.0 6 506 7 173 521 0.0 6 518 3 174 451 0.0 6 448 3 175 421 0.0 6 416 5 176 502 0.0 6 495 5 1 0 0 1 177 393 0.0 6 390 3 178 431 0.0 6 428 2 1 179 366 0.0 6 362 3 0 1 180 312 0.0 6 308 4 181 346 0.0 6 343 2 0 1 182 278 0.0 6 271 5 0 0 0 1 1 183 427 0.0 6 426 1 184 627 0.0 6 619 6 0 1 0 0 1 185 550 0.0 6 539 10 1 186 391 0.0 6 386 4 0 1 187 418 0.0 6 417 1 188 433 0.0 6 430 3 189 438 0.0 6 436 2 190 289 0.0 6 287 1 0 1 191 280 0.0 6 277 3 192 226 0.0 6 226 193 210 0.0 6 210 194 200 0.0 6 194 6 195 154 0.0 6 153 0 0 1 196 211 0.0 6 210 0 0 0 0 0 1 197 176 0.0 6 174 2 198 179 0.0 6 176 2 0 0 0 0 1 199 152 0.0 6 151 1 200 144 0.0 6 143 1 201 128 0.0 6 126 2 202 101 0.0 6 99 2 203 98 0.0 6 97 1 204 104 0.0 6 104 205 87 0.0 6 87 206 94 0.0 6 92 1 0 0 1 207 94 0.0 6 94 208 69 0.0 6 68 1 209 70 0.0 6 70 210 54 0.0 6 53 1 211 66 0.0 6 64 2 212 62 0.0 6 61 1 213 55 0.0 6 55 214 59 0.0 6 59 215 58 0.0 6 58 216 65 0.0 6 65 217 51 0.0 6 51 218 35 0.0 6 35 219 34 0.0 6 32 2 220 25 0.0 6 25 221 33 0.0 6 33 222 24 0.0 6 24 223 30 0.0 6 29 1 224 15 0.0 6 14 1 225 8 0.0 6 8 226 14 0.0 6 14 227 10 0.0 6 10 228 8 0.0 6 8 229 12 0.0 6 11 1 230 4 0.0 6 4 231 11 0.0 6 11 232 10 0.0 6 10 233 6 0.0 6 6 234 8 0.0 6 8 235 2 0.0 6 2 236 5 0.0 6 5 237 2 0.0 6 2 238 4 0.0 6 4 239 6 0.0 6 5 1 240 1 0.0 6 1 241 4 0.0 6 4 242 1 0.0 6 1 243 1 0.0 6 1 244 3 0.0 6 2 1 245 3 0.0 6 3 246 2 0.0 6 2 247 1 0.0 6 1 251 1 0.0 6 1 253 1 0.0 6 1 275 1 0.0 6 1 278 3 0.0 6 3 279 32 0.0 6 32 280 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 811 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 1 0.0 6 1 72 2 0.0 6 2 73 3 0.0 6 3 74 6 0.0 6 6 75 2 0.0 6 2 76 3 0.0 6 3 77 5 0.0 6 5 78 1 0.0 6 1 79 2 0.0 6 2 80 31 0.0 6 31 81 3 0.0 6 3 82 12 0.0 6 12 83 4 0.0 6 4 84 1 0.0 6 1 85 1 0.0 6 1 86 1 0.0 6 1 87 2 0.0 6 2 88 4 0.0 6 4 89 4 0.0 6 4 90 6 0.0 6 6 91 4 0.0 6 4 92 3 0.0 6 3 93 7 0.0 6 7 94 5 0.0 6 5 95 1 0.0 6 1 96 1 0.0 6 1 97 3 0.0 6 3 98 4 0.0 6 4 99 2 0.0 6 2 100 8 0.0 6 8 102 2 0.0 6 2 104 4 0.0 6 3 0 0 0 1 105 3 0.0 6 3 106 4 0.0 6 4 107 6 0.0 6 6 108 3 0.0 6 3 109 6 0.0 6 6 110 8 0.0 6 7 1 111 4 0.0 6 4 112 4 0.0 6 4 114 1 0.0 6 1 115 4 0.0 6 4 116 8 0.0 6 8 117 12 0.0 6 12 118 5 0.0 6 5 119 11 0.0 6 11 120 6 0.0 6 6 121 14 0.0 6 14 122 25 0.0 6 25 123 22 0.0 6 22 125 2 0.0 6 2 126 5 0.0 6 5 127 11 0.0 6 11 128 6 0.0 6 6 129 8 0.0 6 8 130 4 0.0 6 4 131 8 0.0 6 8 132 7 0.0 6 6 1 133 5 0.0 6 5 134 16 0.0 6 16 136 6 0.0 6 6 137 4 0.0 6 4 138 4 0.0 6 4 139 6 0.0 6 6 140 3 0.0 6 3 141 5 0.0 6 5 142 4 0.0 6 4 143 4 0.0 6 4 144 5 0.0 6 5 145 4 0.0 6 4 146 7 0.0 6 7 147 6 0.0 6 6 148 5 0.0 6 5 149 13 0.0 6 13 150 10 0.0 6 10 151 12 0.0 6 12 152 11 0.0 6 11 153 3 0.0 6 3 154 7 0.0 6 7 155 5 0.0 6 5 156 2 0.0 6 2 157 4 0.0 6 4 158 8 0.0 6 8 159 8 0.0 6 8 160 6 0.0 6 6 161 6 0.0 6 6 162 2 0.0 6 2 163 2 0.0 6 2 164 3 0.0 6 3 165 17 0.0 6 17 166 6 0.0 6 6 167 21 0.0 6 20 0 0 0 1 168 10 0.0 6 10 169 4 0.0 6 4 170 7 0.0 6 7 171 9 0.0 6 9 172 1 0.0 6 1 173 10 0.0 6 10 174 6 0.0 6 6 175 3 0.0 6 2 0 0 1 176 11 0.0 6 11 177 6 0.0 6 6 178 8 0.0 6 8 179 15 0.0 6 15 180 7 0.0 6 7 181 6 0.0 6 6 182 6 0.0 6 6 183 6 0.0 6 6 184 4 0.0 6 4 185 13 0.0 6 13 186 3 0.0 6 3 187 1 0.0 6 1 188 12 0.0 6 12 189 15 0.0 6 15 190 4 0.0 6 4 191 8 0.0 6 8 194 1 0.0 6 1 196 3 0.0 6 2 0 0 0 1 197 8 0.0 6 8 198 4 0.0 6 3 1 199 3 0.0 6 3 200 2 0.0 6 2 203 7 0.0 6 7 204 3 0.0 6 3 205 5 0.0 6 5 206 4 0.0 6 4 207 1 0.0 6 1 208 1 0.0 6 1 210 3 0.0 6 3 211 1 0.0 6 1 212 3 0.0 6 3 216 1 0.0 6 1 218 3 0.0 6 3 220 1 0.0 6 1 221 1 0.0 6 1 222 4 0.0 6 4 223 1 0.0 6 1 224 1 0.0 6 1 227 1 0.0 6 1 229 3 0.0 6 3 230 1 0.0 6 1 231 2 0.0 6 2 235 2 0.0 6 2 248 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_16_7_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_16_234_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_16_7_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_16_234_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.91 s (25 µs/read; 2.44 M reads/minute). === Summary === Total read pairs processed: 77,443 Read 1 with adapter: 77,434 (100.0%) Read 2 with adapter: 77,410 (100.0%) == Read fate breakdown == Pairs that were too short: 32 (0.0%) Pairs discarded as untrimmed: 41 (0.1%) Pairs written (passing filters): 77,370 (99.9%) Total basepairs processed: 32,970,677 bp Read 1: 17,974,937 bp Read 2: 14,995,740 bp Total written (filtered): 4,920,818 bp (14.9%) Read 1: 2,526,735 bp Read 2: 2,394,083 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 75089 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1210.0 0 0 2 4 2 302.5 1 0 2 6 1 18.9 1 0 0 1 7 4 4.7 2 1 1 2 9 2 0.3 2 2 14 1 0.0 4 0 1 15 1 0.0 4 1 16 3 0.0 4 3 17 2 0.0 5 2 19 5 0.0 5 5 20 131 0.0 6 129 2 21 86 0.0 6 85 1 22 57 0.0 6 57 23 53 0.0 6 48 4 0 1 24 32 0.0 6 32 25 31 0.0 6 30 1 26 14 0.0 6 12 1 0 0 0 1 27 17 0.0 6 17 28 8 0.0 6 8 29 15 0.0 6 15 30 2 0.0 6 2 31 10 0.0 6 9 1 32 10 0.0 6 10 33 10 0.0 6 7 3 34 5 0.0 6 5 35 8 0.0 6 8 36 10 0.0 6 8 2 37 3 0.0 6 3 38 6 0.0 6 6 39 4 0.0 6 4 40 4 0.0 6 4 41 5 0.0 6 5 42 7 0.0 6 7 43 6 0.0 6 6 44 2 0.0 6 2 45 4 0.0 6 2 2 46 2 0.0 6 1 1 47 4 0.0 6 4 48 9 0.0 6 8 0 0 0 0 1 49 6 0.0 6 6 50 11 0.0 6 11 51 6 0.0 6 5 1 52 7 0.0 6 6 1 53 5 0.0 6 4 0 0 0 0 0 1 54 10 0.0 6 9 0 0 0 0 1 55 8 0.0 6 8 56 3 0.0 6 3 57 4 0.0 6 3 0 0 0 1 58 6 0.0 6 6 59 4 0.0 6 3 0 0 0 0 0 1 60 2 0.0 6 2 61 3 0.0 6 3 62 5 0.0 6 5 63 6 0.0 6 6 64 12 0.0 6 12 65 6 0.0 6 5 1 66 7 0.0 6 6 1 67 3 0.0 6 3 68 8 0.0 6 8 69 7 0.0 6 7 70 4 0.0 6 4 71 8 0.0 6 8 72 12 0.0 6 12 73 10 0.0 6 9 1 74 9 0.0 6 8 0 0 0 0 1 75 11 0.0 6 10 0 1 76 5 0.0 6 5 77 6 0.0 6 5 1 78 2 0.0 6 1 1 79 14 0.0 6 14 80 7 0.0 6 6 1 81 10 0.0 6 9 1 82 10 0.0 6 10 83 8 0.0 6 8 84 10 0.0 6 10 85 9 0.0 6 9 86 9 0.0 6 8 1 87 8 0.0 6 8 88 9 0.0 6 9 89 9 0.0 6 8 1 90 7 0.0 6 6 1 91 4 0.0 6 4 92 8 0.0 6 6 2 93 10 0.0 6 10 94 5 0.0 6 4 1 95 6 0.0 6 6 96 8 0.0 6 8 97 8 0.0 6 7 0 1 98 8 0.0 6 8 99 7 0.0 6 7 100 6 0.0 6 4 2 101 11 0.0 6 11 102 15 0.0 6 15 103 14 0.0 6 14 104 10 0.0 6 10 105 16 0.0 6 16 106 17 0.0 6 17 107 17 0.0 6 16 0 0 0 1 108 20 0.0 6 20 109 9 0.0 6 7 2 110 13 0.0 6 12 1 111 22 0.0 6 21 0 0 0 0 0 1 112 24 0.0 6 24 113 25 0.0 6 24 1 114 21 0.0 6 20 1 115 29 0.0 6 28 1 116 15 0.0 6 15 117 26 0.0 6 25 1 118 20 0.0 6 20 119 17 0.0 6 16 1 120 20 0.0 6 19 1 121 20 0.0 6 20 122 29 0.0 6 27 2 123 23 0.0 6 22 0 0 0 0 0 1 124 18 0.0 6 17 0 1 125 25 0.0 6 25 126 26 0.0 6 25 1 127 20 0.0 6 20 128 27 0.0 6 27 129 36 0.0 6 36 130 16 0.0 6 16 131 43 0.0 6 43 132 34 0.0 6 34 133 37 0.0 6 35 2 134 38 0.0 6 37 0 0 0 0 1 135 41 0.0 6 40 1 136 41 0.0 6 40 1 137 30 0.0 6 30 138 43 0.0 6 43 139 44 0.0 6 43 1 140 54 0.0 6 54 141 184 0.0 6 183 1 142 282 0.0 6 282 143 389 0.0 6 389 144 299 0.0 6 299 145 352 0.0 6 351 1 146 443 0.0 6 442 1 147 514 0.0 6 514 148 732 0.0 6 732 149 475 0.0 6 474 1 150 478 0.0 6 477 1 151 507 0.0 6 505 2 152 540 0.0 6 538 2 153 318 0.0 6 318 154 354 0.0 6 353 1 155 365 0.0 6 363 2 156 495 0.0 6 494 1 157 548 0.0 6 548 158 452 0.0 6 452 159 364 0.0 6 364 160 427 0.0 6 425 2 161 604 0.0 6 602 1 0 0 0 0 1 162 408 0.0 6 408 163 509 0.0 6 508 0 1 164 381 0.0 6 379 1 0 1 165 381 0.0 6 380 1 166 360 0.0 6 359 1 167 479 0.0 6 478 1 168 735 0.0 6 733 2 169 719 0.0 6 717 1 0 0 1 170 496 0.0 6 495 1 171 458 0.0 6 456 2 172 383 0.0 6 382 1 173 778 0.0 6 777 1 174 399 0.0 6 397 2 175 257 0.0 6 257 176 516 0.0 6 513 2 0 0 0 0 1 177 655 0.0 6 654 1 178 407 0.0 6 404 2 0 0 0 1 179 749 0.0 6 749 180 573 0.0 6 573 181 561 0.0 6 561 182 410 0.0 6 410 183 589 0.0 6 587 2 184 1147 0.0 6 1141 6 185 829 0.0 6 826 2 0 0 0 1 186 482 0.0 6 482 187 566 0.0 6 566 188 462 0.0 6 461 1 189 677 0.0 6 675 1 1 190 434 0.0 6 433 1 191 436 0.0 6 435 1 192 897 0.0 6 894 3 193 572 0.0 6 571 1 194 922 0.0 6 919 2 0 1 195 958 0.0 6 956 1 0 0 0 0 1 196 761 0.0 6 757 4 197 832 0.0 6 831 1 198 910 0.0 6 906 1 1 0 0 1 1 199 782 0.0 6 779 3 200 900 0.0 6 898 2 201 889 0.0 6 886 3 202 910 0.0 6 910 203 901 0.0 6 900 1 204 655 0.0 6 652 3 205 778 0.0 6 776 2 206 937 0.0 6 933 4 207 1061 0.0 6 1057 3 0 0 0 0 1 208 912 0.0 6 911 1 209 967 0.0 6 967 210 618 0.0 6 616 1 0 0 1 211 704 0.0 6 700 3 1 212 888 0.0 6 886 2 213 906 0.0 6 905 1 214 827 0.0 6 826 1 215 1260 0.0 6 1258 2 216 1287 0.0 6 1280 6 0 0 0 0 1 217 926 0.0 6 923 3 218 914 0.0 6 912 2 219 776 0.0 6 772 4 220 782 0.0 6 778 3 0 0 0 1 221 643 0.0 6 641 2 222 626 0.0 6 624 2 223 763 0.0 6 761 2 224 761 0.0 6 757 3 0 0 1 225 660 0.0 6 657 3 226 682 0.0 6 679 3 227 744 0.0 6 743 1 228 653 0.0 6 650 2 0 0 0 0 1 229 587 0.0 6 587 230 627 0.0 6 626 1 231 690 0.0 6 690 232 678 0.0 6 675 3 233 630 0.0 6 627 3 234 617 0.0 6 613 4 235 538 0.0 6 534 2 0 1 0 0 1 236 519 0.0 6 517 2 237 590 0.0 6 589 1 238 550 0.0 6 547 3 239 501 0.0 6 501 240 505 0.0 6 504 1 241 381 0.0 6 381 242 416 0.0 6 416 243 401 0.0 6 399 2 244 378 0.0 6 375 3 245 281 0.0 6 279 2 246 264 0.0 6 261 3 247 281 0.0 6 281 248 271 0.0 6 270 1 249 254 0.0 6 253 0 0 1 250 234 0.0 6 233 0 1 251 237 0.0 6 232 4 0 0 0 0 1 252 228 0.0 6 227 1 253 219 0.0 6 218 1 254 220 0.0 6 220 255 171 0.0 6 169 1 0 0 0 0 1 256 192 0.0 6 191 0 0 0 1 257 185 0.0 6 183 2 258 184 0.0 6 184 259 220 0.0 6 218 1 0 1 260 201 0.0 6 200 1 261 178 0.0 6 174 4 262 150 0.0 6 148 2 263 139 0.0 6 139 264 157 0.0 6 157 265 142 0.0 6 141 1 266 136 0.0 6 133 2 0 0 0 0 1 267 134 0.0 6 133 1 268 137 0.0 6 137 269 132 0.0 6 130 1 1 270 160 0.0 6 160 271 104 0.0 6 102 2 272 116 0.0 6 115 1 273 88 0.0 6 87 1 274 137 0.0 6 137 275 183 0.0 6 181 2 276 179 0.0 6 177 1 0 0 1 277 211 0.0 6 210 0 0 0 0 1 278 218 0.0 6 215 3 279 747 0.0 6 739 7 0 0 0 0 1 280 1094 0.0 6 1085 8 0 0 1 281 12 0.0 6 12 282 1 0.0 6 1 283 1 0.0 6 1 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2345 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 55 3 0.0 6 3 60 1 0.0 6 0 1 61 45 0.0 6 0 44 1 62 54 0.0 6 53 1 70 1 0.0 6 1 71 11 0.0 6 11 72 6 0.0 6 6 73 14 0.0 6 14 74 11 0.0 6 11 75 17 0.0 6 17 76 12 0.0 6 12 77 18 0.0 6 18 78 18 0.0 6 18 79 16 0.0 6 16 80 12 0.0 6 12 81 19 0.0 6 19 82 16 0.0 6 16 83 7 0.0 6 7 84 13 0.0 6 13 85 9 0.0 6 9 86 18 0.0 6 18 87 12 0.0 6 12 88 12 0.0 6 12 89 10 0.0 6 10 90 12 0.0 6 12 91 15 0.0 6 15 92 13 0.0 6 13 93 4 0.0 6 4 94 12 0.0 6 12 95 11 0.0 6 11 96 12 0.0 6 12 97 24 0.0 6 24 98 26 0.0 6 26 99 23 0.0 6 23 100 11 0.0 6 11 101 15 0.0 6 15 102 25 0.0 6 25 103 15 0.0 6 15 104 12 0.0 6 12 105 5 0.0 6 5 106 16 0.0 6 16 107 22 0.0 6 22 108 10 0.0 6 10 109 21 0.0 6 21 110 17 0.0 6 17 111 14 0.0 6 14 112 10 0.0 6 10 113 23 0.0 6 23 114 41 0.0 6 41 115 30 0.0 6 30 116 15 0.0 6 15 117 10 0.0 6 10 118 16 0.0 6 15 1 119 17 0.0 6 17 120 9 0.0 6 9 121 14 0.0 6 14 122 14 0.0 6 14 123 4 0.0 6 4 124 20 0.0 6 20 125 15 0.0 6 15 126 16 0.0 6 16 127 9 0.0 6 9 128 11 0.0 6 11 129 17 0.0 6 17 130 12 0.0 6 12 131 25 0.0 6 25 132 24 0.0 6 24 133 25 0.0 6 25 134 5 0.0 6 5 135 16 0.0 6 16 136 21 0.0 6 21 137 9 0.0 6 9 138 12 0.0 6 12 139 18 0.0 6 18 140 15 0.0 6 15 141 13 0.0 6 13 142 22 0.0 6 22 143 26 0.0 6 26 144 15 0.0 6 15 145 35 0.0 6 35 146 42 0.0 6 42 147 24 0.0 6 24 148 12 0.0 6 12 149 17 0.0 6 17 150 14 0.0 6 14 151 9 0.0 6 9 152 14 0.0 6 14 153 21 0.0 6 21 154 23 0.0 6 23 155 18 0.0 6 18 156 8 0.0 6 8 157 12 0.0 6 12 158 7 0.0 6 7 159 12 0.0 6 12 160 21 0.0 6 21 161 28 0.0 6 28 162 27 0.0 6 27 163 18 0.0 6 18 164 13 0.0 6 13 165 19 0.0 6 19 166 24 0.0 6 24 167 22 0.0 6 22 168 14 0.0 6 14 169 15 0.0 6 15 170 23 0.0 6 23 171 14 0.0 6 14 172 25 0.0 6 25 173 11 0.0 6 11 174 22 0.0 6 22 175 11 0.0 6 11 176 12 0.0 6 12 177 20 0.0 6 20 178 25 0.0 6 25 179 14 0.0 6 14 180 8 0.0 6 7 0 0 0 0 0 1 181 6 0.0 6 6 182 10 0.0 6 10 183 8 0.0 6 8 184 5 0.0 6 5 185 12 0.0 6 12 186 16 0.0 6 16 187 10 0.0 6 10 188 10 0.0 6 10 189 10 0.0 6 10 190 10 0.0 6 10 191 11 0.0 6 11 192 5 0.0 6 5 193 14 0.0 6 14 194 7 0.0 6 7 195 8 0.0 6 8 196 12 0.0 6 12 197 6 0.0 6 6 198 4 0.0 6 4 199 9 0.0 6 9 200 9 0.0 6 9 201 8 0.0 6 8 202 17 0.0 6 17 203 4 0.0 6 4 204 6 0.0 6 6 205 6 0.0 6 6 206 4 0.0 6 4 207 2 0.0 6 2 208 5 0.0 6 5 209 3 0.0 6 3 210 1 0.0 6 1 211 4 0.0 6 4 212 3 0.0 6 3 213 5 0.0 6 5 214 3 0.0 6 3 215 2 0.0 6 2 216 1 0.0 6 1 217 15 0.0 6 15 218 2 0.0 6 2 219 5 0.0 6 5 220 6 0.0 6 6 221 5 0.0 6 5 222 10 0.0 6 10 223 7 0.0 6 7 224 4 0.0 6 4 225 4 0.0 6 4 226 4 0.0 6 4 228 5 0.0 6 5 229 8 0.0 6 8 230 4 0.0 6 4 231 7 0.0 6 7 232 3 0.0 6 3 233 5 0.0 6 5 234 3 0.0 6 3 235 4 0.0 6 4 236 5 0.0 6 5 237 3 0.0 6 3 238 4 0.0 6 4 239 3 0.0 6 3 240 5 0.0 6 5 241 2 0.0 6 2 242 1 0.0 6 1 243 2 0.0 6 2 244 4 0.0 6 4 245 4 0.0 6 4 246 1 0.0 6 1 247 1 0.0 6 1 248 1 0.0 6 1 249 5 0.0 6 5 250 5 0.0 6 5 252 3 0.0 6 3 253 2 0.0 6 2 254 1 0.0 6 1 255 1 0.0 6 1 256 1 0.0 6 1 258 1 0.0 6 1 259 1 0.0 6 1 260 1 0.0 6 1 261 1 0.0 6 1 262 2 0.0 6 2 263 2 0.0 6 2 265 1 0.0 6 1 266 2 0.0 6 2 267 2 0.0 6 2 268 2 0.0 6 2 269 1 0.0 6 1 270 1 0.0 6 1 271 4 0.0 6 4 272 3 0.0 6 3 273 1 0.0 6 1 274 1 0.0 6 1 275 2 0.0 6 2 277 1 0.0 6 1 280 1 0.0 6 1 284 1 0.0 6 1 285 3 0.0 6 3 286 2 0.0 6 2 288 2 0.0 6 2 298 1 0.0 6 1 302 1 0.0 6 1 303 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 75257 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 9 1 0.3 2 1 12 1 0.0 3 1 13 8 0.0 3 8 14 5 0.0 4 3 1 0 1 16 7 0.0 4 7 17 18 0.0 5 17 1 18 15 0.0 5 13 2 19 9 0.0 5 8 1 20 110 0.0 6 105 4 1 21 62 0.0 6 61 0 1 22 45 0.0 6 43 2 23 34 0.0 6 33 1 24 18 0.0 6 16 2 25 17 0.0 6 15 1 1 26 14 0.0 6 12 2 27 9 0.0 6 8 1 28 16 0.0 6 15 1 29 11 0.0 6 11 30 10 0.0 6 9 1 31 11 0.0 6 11 32 11 0.0 6 8 3 33 10 0.0 6 8 2 34 15 0.0 6 14 1 35 8 0.0 6 7 1 36 17 0.0 6 17 37 12 0.0 6 10 2 38 3 0.0 6 3 39 7 0.0 6 6 1 40 5 0.0 6 5 41 6 0.0 6 6 42 17 0.0 6 17 43 8 0.0 6 7 1 44 6 0.0 6 3 2 0 0 0 0 1 45 11 0.0 6 10 1 46 9 0.0 6 8 1 47 10 0.0 6 8 2 48 8 0.0 6 7 1 49 9 0.0 6 9 50 18 0.0 6 17 1 51 17 0.0 6 14 3 52 17 0.0 6 17 53 19 0.0 6 18 1 54 16 0.0 6 15 1 55 17 0.0 6 17 56 10 0.0 6 9 1 57 14 0.0 6 14 58 14 0.0 6 13 1 59 20 0.0 6 17 2 0 1 60 18 0.0 6 15 3 61 24 0.0 6 22 2 62 20 0.0 6 20 63 17 0.0 6 16 1 64 18 0.0 6 18 65 24 0.0 6 24 66 26 0.0 6 24 2 67 27 0.0 6 25 2 68 31 0.0 6 30 1 69 156 0.0 6 148 5 2 0 1 70 48 0.0 6 45 3 71 61 0.0 6 59 2 72 38 0.0 6 37 1 73 49 0.0 6 47 1 1 74 29 0.0 6 28 1 75 27 0.0 6 26 0 1 76 26 0.0 6 24 2 77 38 0.0 6 36 1 0 0 1 78 21 0.0 6 21 79 28 0.0 6 28 80 32 0.0 6 32 81 38 0.0 6 37 1 82 37 0.0 6 36 1 83 35 0.0 6 35 84 39 0.0 6 37 2 85 66 0.0 6 65 1 86 21 0.0 6 21 87 40 0.0 6 38 1 1 88 34 0.0 6 33 1 89 29 0.0 6 29 90 33 0.0 6 32 1 91 49 0.0 6 48 1 92 46 0.0 6 46 93 51 0.0 6 49 2 94 62 0.0 6 60 2 95 50 0.0 6 46 4 96 46 0.0 6 46 97 54 0.0 6 53 1 98 62 0.0 6 60 1 1 99 56 0.0 6 56 100 59 0.0 6 57 2 101 48 0.0 6 47 1 102 56 0.0 6 55 1 103 64 0.0 6 63 1 104 68 0.0 6 67 0 0 0 1 105 84 0.0 6 80 4 106 130 0.0 6 129 1 107 68 0.0 6 65 3 108 68 0.0 6 66 2 109 91 0.0 6 87 2 0 1 1 110 81 0.0 6 78 2 1 111 71 0.0 6 69 2 112 84 0.0 6 84 113 102 0.0 6 101 0 0 0 1 114 115 0.0 6 113 1 1 115 95 0.0 6 91 4 116 127 0.0 6 124 3 117 134 0.0 6 130 3 1 118 102 0.0 6 100 2 119 131 0.0 6 128 2 0 0 1 120 140 0.0 6 137 3 121 128 0.0 6 124 2 0 1 0 1 122 175 0.0 6 170 5 123 152 0.0 6 145 7 124 131 0.0 6 129 1 0 0 1 125 172 0.0 6 168 4 126 173 0.0 6 169 4 127 163 0.0 6 160 2 1 128 202 0.0 6 199 3 129 269 0.0 6 235 32 1 1 130 308 0.0 6 298 9 0 0 0 1 131 280 0.0 6 275 2 1 1 0 1 132 290 0.0 6 287 2 1 133 325 0.0 6 321 4 134 333 0.0 6 329 4 135 332 0.0 6 326 4 1 0 0 0 1 136 406 0.0 6 401 3 0 0 0 1 1 137 409 0.0 6 408 1 138 429 0.0 6 427 2 139 515 0.0 6 510 5 140 584 0.0 6 578 6 141 681 0.0 6 677 2 0 0 0 1 1 142 633 0.0 6 630 2 0 1 143 764 0.0 6 761 3 144 888 0.0 6 880 8 145 932 0.0 6 922 8 0 1 1 146 1123 0.0 6 1113 9 0 0 1 147 914 0.0 6 905 6 2 0 0 0 1 148 964 0.0 6 955 8 0 0 0 0 1 149 1111 0.0 6 1103 6 0 0 1 0 1 150 1213 0.0 6 1205 8 151 1031 0.0 6 1020 10 0 1 152 1476 0.0 6 1460 11 2 1 2 153 1171 0.0 6 1157 10 0 2 2 154 1455 0.0 6 1440 12 0 0 1 1 1 155 1519 0.0 6 1506 11 0 1 0 0 1 156 1257 0.0 6 1248 8 1 157 1281 0.0 6 1267 11 1 1 1 158 1166 0.0 6 1159 5 0 0 2 159 1381 0.0 6 1371 7 1 0 0 2 160 1364 0.0 6 1354 9 0 1 161 1166 0.0 6 1156 9 1 162 1220 0.0 6 1212 6 1 0 1 163 1069 0.0 6 1062 6 0 0 0 1 164 1042 0.0 6 1035 6 0 0 0 0 1 165 1176 0.0 6 1173 2 0 1 166 1180 0.0 6 1171 7 0 1 0 0 1 167 1118 0.0 6 1115 3 168 1171 0.0 6 1162 7 0 0 1 0 1 169 1025 0.0 6 1019 5 0 0 0 1 170 1309 0.0 6 1300 8 1 171 1263 0.0 6 1249 13 0 1 172 1066 0.0 6 1059 5 0 2 173 1142 0.0 6 1128 13 0 0 0 0 1 174 1257 0.0 6 1250 7 175 1423 0.0 6 1412 9 2 176 1189 0.0 6 1181 5 0 1 1 0 1 177 1167 0.0 6 1159 8 178 1238 0.0 6 1234 3 0 1 179 1009 0.0 6 997 11 0 1 180 998 0.0 6 992 5 1 181 896 0.0 6 892 3 0 0 1 182 822 0.0 6 819 2 1 183 816 0.0 6 810 5 0 0 0 0 1 184 724 0.0 6 718 5 0 0 0 0 1 185 959 0.0 6 955 3 0 0 0 0 1 186 1227 0.0 6 1222 2 0 2 0 1 187 1202 0.0 6 1188 13 0 0 1 188 836 0.0 6 827 5 0 1 2 0 1 189 859 0.0 6 858 1 190 802 0.0 6 799 3 191 758 0.0 6 754 2 1 0 1 192 722 0.0 6 721 1 193 745 0.0 6 738 5 0 0 1 1 194 508 0.0 6 507 1 195 506 0.0 6 503 3 196 490 0.0 6 485 5 197 399 0.0 6 395 3 0 0 0 0 1 198 517 0.0 6 515 2 199 463 0.0 6 461 2 200 407 0.0 6 406 0 1 201 296 0.0 6 294 2 202 355 0.0 6 352 3 203 268 0.0 6 261 6 0 0 0 1 204 323 0.0 6 322 1 205 301 0.0 6 299 2 206 256 0.0 6 255 1 207 221 0.0 6 217 3 0 0 0 1 208 252 0.0 6 252 209 219 0.0 6 217 2 210 189 0.0 6 188 1 211 198 0.0 6 198 212 142 0.0 6 140 2 213 166 0.0 6 165 1 214 165 0.0 6 162 2 0 0 0 1 215 157 0.0 6 156 0 0 1 216 173 0.0 6 169 3 0 0 0 0 1 217 115 0.0 6 115 218 126 0.0 6 125 1 219 107 0.0 6 107 220 95 0.0 6 95 221 73 0.0 6 73 222 67 0.0 6 66 0 0 1 223 84 0.0 6 81 2 0 0 0 1 224 64 0.0 6 63 1 225 73 0.0 6 73 226 67 0.0 6 67 227 51 0.0 6 51 228 48 0.0 6 47 0 0 1 229 32 0.0 6 32 230 42 0.0 6 40 1 0 0 1 231 21 0.0 6 20 1 232 22 0.0 6 22 233 21 0.0 6 21 234 23 0.0 6 21 2 235 17 0.0 6 17 236 11 0.0 6 10 1 237 6 0.0 6 6 238 11 0.0 6 11 239 5 0.0 6 5 240 5 0.0 6 5 241 7 0.0 6 7 242 12 0.0 6 12 243 13 0.0 6 13 244 2 0.0 6 2 245 6 0.0 6 6 246 1 0.0 6 1 247 2 0.0 6 2 248 1 0.0 6 1 249 9 0.0 6 7 1 1 250 2 0.0 6 2 251 1 0.0 6 1 252 2 0.0 6 2 253 3 0.0 6 3 254 1 0.0 6 1 277 1 0.0 6 1 278 1 0.0 6 1 280 5 0.0 6 5 281 55 0.0 6 53 1 0 1 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2153 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 57 1 0.0 6 1 59 1 0.0 6 1 65 1 0.0 6 0 1 69 3 0.0 6 3 70 3 0.0 6 3 71 11 0.0 6 11 72 16 0.0 6 16 73 9 0.0 6 9 74 13 0.0 6 13 75 12 0.0 6 12 76 25 0.0 6 25 77 34 0.0 6 34 78 14 0.0 6 14 79 14 0.0 6 14 80 19 0.0 6 19 81 33 0.0 6 33 82 8 0.0 6 8 83 15 0.0 6 14 1 84 17 0.0 6 16 1 85 14 0.0 6 14 86 17 0.0 6 17 87 10 0.0 6 10 88 14 0.0 6 14 89 17 0.0 6 17 90 22 0.0 6 22 91 18 0.0 6 17 1 92 16 0.0 6 16 93 5 0.0 6 4 0 0 0 1 94 10 0.0 6 10 95 8 0.0 6 8 96 26 0.0 6 26 97 24 0.0 6 24 98 24 0.0 6 24 99 19 0.0 6 19 100 7 0.0 6 7 101 18 0.0 6 17 0 0 0 1 102 34 0.0 6 34 103 12 0.0 6 12 104 7 0.0 6 7 105 13 0.0 6 13 106 21 0.0 6 21 107 8 0.0 6 8 108 27 0.0 6 27 109 11 0.0 6 11 110 15 0.0 6 15 111 13 0.0 6 13 112 24 0.0 6 24 113 34 0.0 6 34 114 27 0.0 6 27 115 12 0.0 6 12 116 18 0.0 6 18 117 13 0.0 6 11 0 0 0 2 118 19 0.0 6 19 119 8 0.0 6 8 120 12 0.0 6 12 121 25 0.0 6 25 122 15 0.0 6 15 123 36 0.0 6 36 124 23 0.0 6 23 125 13 0.0 6 13 126 12 0.0 6 12 127 13 0.0 6 13 128 5 0.0 6 5 129 32 0.0 6 32 130 29 0.0 6 29 131 32 0.0 6 32 132 21 0.0 6 21 133 10 0.0 6 10 134 15 0.0 6 15 135 22 0.0 6 21 1 136 12 0.0 6 12 137 14 0.0 6 14 138 20 0.0 6 20 139 8 0.0 6 8 140 17 0.0 6 17 141 22 0.0 6 22 142 19 0.0 6 19 143 19 0.0 6 19 144 38 0.0 6 38 145 35 0.0 6 35 146 13 0.0 6 13 147 17 0.0 6 17 148 15 0.0 6 15 149 17 0.0 6 17 150 14 0.0 6 12 2 151 17 0.0 6 17 152 28 0.0 6 28 153 23 0.0 6 23 154 11 0.0 6 11 155 11 0.0 6 10 0 0 1 156 17 0.0 6 17 157 12 0.0 6 11 0 0 0 1 158 14 0.0 6 14 159 24 0.0 6 24 160 26 0.0 6 26 161 28 0.0 6 28 162 12 0.0 6 11 1 163 18 0.0 6 18 164 10 0.0 6 10 165 34 0.0 6 34 166 25 0.0 6 24 1 167 27 0.0 6 27 168 20 0.0 6 20 169 29 0.0 6 29 170 11 0.0 6 11 171 12 0.0 6 12 172 18 0.0 6 18 173 15 0.0 6 15 174 7 0.0 6 7 175 13 0.0 6 13 176 12 0.0 6 12 177 25 0.0 6 25 178 14 0.0 6 14 179 6 0.0 6 5 0 0 0 1 180 5 0.0 6 5 181 4 0.0 6 4 182 10 0.0 6 10 183 9 0.0 6 9 184 9 0.0 6 9 185 11 0.0 6 11 186 11 0.0 6 11 187 6 0.0 6 6 188 4 0.0 6 4 189 4 0.0 6 4 190 14 0.0 6 13 0 0 0 1 191 5 0.0 6 5 192 8 0.0 6 8 193 7 0.0 6 7 194 2 0.0 6 2 195 6 0.0 6 6 196 5 0.0 6 5 198 2 0.0 6 2 199 14 0.0 6 14 200 2 0.0 6 2 201 4 0.0 6 4 202 3 0.0 6 3 203 2 0.0 6 2 204 5 0.0 6 5 205 3 0.0 6 3 206 3 0.0 6 3 207 3 0.0 6 3 208 4 0.0 6 4 209 3 0.0 6 3 211 1 0.0 6 1 212 3 0.0 6 3 213 3 0.0 6 3 214 5 0.0 6 4 0 0 0 1 215 3 0.0 6 3 216 4 0.0 6 4 217 2 0.0 6 2 218 1 0.0 6 1 219 2 0.0 6 2 220 1 0.0 6 1 221 1 0.0 6 1 222 1 0.0 6 1 223 2 0.0 6 2 226 1 0.0 6 1 227 1 0.0 6 1 228 2 0.0 6 2 229 1 0.0 6 1 237 1 0.0 6 1 245 1 0.0 6 1 261 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_17_8_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_17_235_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_17_8_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_17_235_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.16 s (24 µs/read; 2.48 M reads/minute). === Summary === Total read pairs processed: 89,461 Read 1 with adapter: 89,452 (100.0%) Read 2 with adapter: 89,438 (100.0%) == Read fate breakdown == Pairs that were too short: 12 (0.0%) Pairs discarded as untrimmed: 32 (0.0%) Pairs written (passing filters): 89,417 (100.0%) Total basepairs processed: 42,469,050 bp Read 1: 23,972,003 bp Read 2: 18,497,047 bp Total written (filtered): 5,495,732 bp (12.9%) Read 1: 2,842,015 bp Read 2: 2,653,717 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 88259 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 3 349.5 1 1 2 6 2 21.8 1 0 0 2 7 1 5.5 2 1 9 2 0.3 2 1 1 15 4 0.0 4 4 16 3 0.0 4 2 1 17 4 0.0 5 4 19 2 0.0 5 2 20 51 0.0 6 49 2 21 41 0.0 6 38 3 22 29 0.0 6 28 1 23 22 0.0 6 22 24 16 0.0 6 16 25 24 0.0 6 21 3 26 11 0.0 6 11 27 10 0.0 6 10 28 10 0.0 6 9 0 0 0 1 29 9 0.0 6 9 30 5 0.0 6 4 1 31 2 0.0 6 2 32 4 0.0 6 4 33 9 0.0 6 9 34 5 0.0 6 3 1 0 1 35 6 0.0 6 5 1 36 3 0.0 6 3 37 3 0.0 6 3 38 4 0.0 6 4 39 1 0.0 6 1 40 4 0.0 6 4 41 5 0.0 6 5 42 2 0.0 6 2 43 6 0.0 6 5 0 1 44 6 0.0 6 6 45 4 0.0 6 4 46 2 0.0 6 2 47 4 0.0 6 2 2 48 5 0.0 6 5 49 2 0.0 6 1 1 50 7 0.0 6 6 1 51 5 0.0 6 5 52 2 0.0 6 2 53 4 0.0 6 3 1 54 4 0.0 6 4 55 3 0.0 6 3 56 4 0.0 6 4 57 6 0.0 6 6 58 4 0.0 6 4 59 3 0.0 6 3 60 1 0.0 6 1 62 6 0.0 6 6 63 4 0.0 6 3 1 64 8 0.0 6 8 65 1 0.0 6 1 67 2 0.0 6 2 68 6 0.0 6 5 1 69 8 0.0 6 8 70 2 0.0 6 2 71 7 0.0 6 7 72 10 0.0 6 10 73 4 0.0 6 4 74 6 0.0 6 6 75 5 0.0 6 5 76 12 0.0 6 11 1 77 7 0.0 6 7 78 7 0.0 6 5 2 79 4 0.0 6 4 80 5 0.0 6 5 81 9 0.0 6 9 82 6 0.0 6 6 83 8 0.0 6 8 84 10 0.0 6 10 85 6 0.0 6 6 86 7 0.0 6 7 87 5 0.0 6 5 88 5 0.0 6 5 89 3 0.0 6 3 90 4 0.0 6 3 1 91 6 0.0 6 5 0 1 92 11 0.0 6 11 93 3 0.0 6 3 95 6 0.0 6 4 0 1 0 0 0 1 96 2 0.0 6 2 97 8 0.0 6 7 1 98 10 0.0 6 10 99 7 0.0 6 7 100 11 0.0 6 10 0 0 1 101 15 0.0 6 15 102 19 0.0 6 18 1 103 7 0.0 6 7 104 10 0.0 6 9 1 105 10 0.0 6 10 106 21 0.0 6 20 0 1 107 23 0.0 6 22 1 108 20 0.0 6 19 1 109 10 0.0 6 10 110 2 0.0 6 2 111 11 0.0 6 11 112 9 0.0 6 9 113 15 0.0 6 15 114 18 0.0 6 16 2 115 21 0.0 6 21 116 13 0.0 6 13 117 21 0.0 6 19 2 118 18 0.0 6 18 119 17 0.0 6 16 1 120 23 0.0 6 23 121 33 0.0 6 31 1 1 122 41 0.0 6 41 123 39 0.0 6 37 0 2 124 19 0.0 6 19 125 20 0.0 6 20 126 19 0.0 6 16 3 127 18 0.0 6 17 1 128 26 0.0 6 24 1 0 0 1 129 40 0.0 6 39 1 130 28 0.0 6 28 131 36 0.0 6 36 132 65 0.0 6 64 1 133 32 0.0 6 31 0 1 134 30 0.0 6 29 1 135 29 0.0 6 28 1 136 41 0.0 6 40 1 137 41 0.0 6 41 138 51 0.0 6 50 1 139 120 0.0 6 118 2 140 519 0.0 6 518 1 141 1433 0.0 6 1430 3 142 571 0.0 6 570 1 143 113 0.0 6 113 144 53 0.0 6 52 1 145 91 0.0 6 90 1 146 299 0.0 6 298 1 147 186 0.0 6 186 148 866 0.0 6 863 1 0 0 1 0 1 149 576 0.0 6 573 3 150 142 0.0 6 140 2 151 88 0.0 6 86 2 152 112 0.0 6 110 2 153 177 0.0 6 177 154 1144 0.0 6 1142 2 155 1231 0.0 6 1226 3 1 0 1 156 1270 0.0 6 1267 3 157 199 0.0 6 199 158 53 0.0 6 53 159 64 0.0 6 63 1 160 98 0.0 6 94 4 161 310 0.0 6 309 1 162 152 0.0 6 150 2 163 937 0.0 6 930 7 164 258 0.0 6 255 2 0 0 0 0 1 165 1743 0.0 6 1742 1 166 309 0.0 6 309 167 71 0.0 6 70 1 168 64 0.0 6 63 1 169 83 0.0 6 82 1 170 142 0.0 6 141 1 171 251 0.0 6 251 172 2475 0.0 6 2473 2 173 384 0.0 6 382 2 174 1733 0.0 6 1730 3 175 187 0.0 6 186 1 176 56 0.0 6 54 1 1 177 67 0.0 6 65 1 0 0 0 1 178 42 0.0 6 38 4 179 37 0.0 6 36 1 180 50 0.0 6 49 1 181 53 0.0 6 52 1 182 72 0.0 6 70 2 183 75 0.0 6 74 1 184 101 0.0 6 93 8 185 114 0.0 6 111 2 1 186 128 0.0 6 119 9 187 252 0.0 6 250 2 188 1245 0.0 6 1241 4 189 198 0.0 6 194 4 190 422 0.0 6 419 3 191 152 0.0 6 145 5 2 192 91 0.0 6 85 6 193 109 0.0 6 101 8 194 126 0.0 6 122 3 1 195 163 0.0 6 155 8 196 214 0.0 6 208 5 0 0 0 1 197 226 0.0 6 217 9 198 329 0.0 6 320 8 1 199 909 0.0 6 905 3 0 1 200 197 0.0 6 191 5 1 201 154 0.0 6 148 6 202 198 0.0 6 192 6 203 185 0.0 6 178 7 204 218 0.0 6 209 9 205 217 0.0 6 213 4 206 287 0.0 6 279 8 207 259 0.0 6 249 9 1 208 254 0.0 6 245 9 209 266 0.0 6 263 3 210 180 0.0 6 177 2 1 211 156 0.0 6 152 4 212 140 0.0 6 139 1 213 160 0.0 6 152 8 214 158 0.0 6 152 5 0 1 215 181 0.0 6 172 8 0 0 0 0 1 216 439 0.0 6 432 7 217 501 0.0 6 494 6 0 0 0 0 1 218 184 0.0 6 178 6 219 160 0.0 6 156 4 220 164 0.0 6 158 6 221 160 0.0 6 155 4 0 0 0 0 1 222 123 0.0 6 119 4 223 89 0.0 6 84 5 224 125 0.0 6 120 4 1 225 194 0.0 6 186 6 2 226 257 0.0 6 249 8 227 245 0.0 6 236 9 228 218 0.0 6 209 9 229 247 0.0 6 240 7 230 148 0.0 6 147 1 231 150 0.0 6 145 4 1 232 198 0.0 6 194 4 233 189 0.0 6 187 2 234 195 0.0 6 192 3 235 160 0.0 6 157 3 236 155 0.0 6 149 5 1 237 187 0.0 6 182 4 1 238 220 0.0 6 214 6 239 312 0.0 6 300 12 240 427 0.0 6 410 14 1 1 1 241 434 0.0 6 425 9 242 361 0.0 6 350 11 243 349 0.0 6 337 12 244 371 0.0 6 358 12 0 0 1 245 334 0.0 6 314 18 2 246 196 0.0 6 194 1 0 1 247 201 0.0 6 193 8 248 313 0.0 6 298 15 249 463 0.0 6 448 13 1 1 250 462 0.0 6 450 12 251 246 0.0 6 234 12 252 193 0.0 6 190 2 1 253 219 0.0 6 203 16 254 259 0.0 6 246 13 255 194 0.0 6 183 11 256 162 0.0 6 155 7 257 163 0.0 6 157 6 258 233 0.0 6 224 9 259 241 0.0 6 234 7 260 256 0.0 6 243 13 261 180 0.0 6 173 7 262 228 0.0 6 218 8 2 263 326 0.0 6 316 9 1 264 160 0.0 6 153 7 265 159 0.0 6 150 9 266 108 0.0 6 101 7 267 154 0.0 6 143 10 0 1 268 181 0.0 6 170 11 269 155 0.0 6 148 5 1 1 270 157 0.0 6 146 9 1 1 271 338 0.0 6 325 12 1 272 435 0.0 6 415 16 3 1 273 280 0.0 6 267 11 1 1 274 419 0.0 6 406 12 0 0 0 0 1 275 340 0.0 6 306 31 3 276 1137 0.0 6 1090 45 1 0 1 277 1312 0.0 6 1277 33 0 1 0 0 1 278 777 0.0 6 740 34 1 2 279 729 0.0 6 635 78 11 3 1 1 280 40980 0.0 6 39758 1204 14 0 1 2 1 281 135 0.0 6 117 15 2 0 0 1 282 7 0.0 6 6 1 283 3 0.0 6 1 0 0 1 1 284 3 0.0 6 3 285 1 0.0 6 1 286 1 0.0 6 1 288 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1193 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 5 0.0 6 5 71 36 0.0 6 36 72 52 0.0 6 52 73 14 0.0 6 14 75 1 0.0 6 1 76 4 0.0 6 4 77 14 0.0 6 14 78 17 0.0 6 17 79 34 0.0 6 34 80 17 0.0 6 17 81 1 0.0 6 1 83 2 0.0 6 2 84 19 0.0 6 19 85 50 0.0 6 50 86 60 0.0 6 60 87 52 0.0 6 51 1 88 2 0.0 6 2 91 4 0.0 6 4 92 3 0.0 6 3 93 8 0.0 6 8 94 30 0.0 6 30 95 24 0.0 6 24 96 83 0.0 6 83 97 4 0.0 6 4 100 1 0.0 6 0 1 101 5 0.0 6 5 102 39 0.0 6 39 103 100 0.0 6 100 104 34 0.0 6 34 105 77 0.0 6 77 106 2 0.0 6 2 117 2 0.0 6 1 1 118 18 0.0 6 18 119 47 0.0 6 46 1 120 14 0.0 6 14 121 17 0.0 6 17 122 1 0.0 6 1 126 1 0.0 6 1 127 1 0.0 6 1 129 16 0.0 6 16 130 44 0.0 6 44 131 5 0.0 6 5 137 1 0.0 6 1 167 3 0.0 6 3 168 3 0.0 6 3 169 9 0.0 6 9 170 26 0.0 6 26 172 5 0.0 6 5 173 11 0.0 6 11 177 1 0.0 6 0 1 180 17 0.0 6 17 181 50 0.0 6 50 182 2 0.0 6 2 187 1 0.0 6 1 188 1 0.0 6 1 191 1 0.0 6 1 192 2 0.0 6 2 218 1 0.0 6 1 219 3 0.0 6 3 230 1 0.0 6 1 238 2 0.0 6 2 246 1 0.0 6 1 248 3 0.0 6 3 249 22 0.0 6 22 250 53 0.0 6 53 251 2 0.0 6 2 271 1 0.0 6 1 272 4 0.0 6 4 273 1 0.0 6 1 299 1 0.0 6 1 300 5 0.0 6 5 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 88259 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 349.5 1 1 5 1 87.4 1 1 7 2 5.5 2 1 1 10 1 0.1 3 1 11 6 0.0 3 4 1 0 1 12 10 0.0 3 10 13 8 0.0 3 8 14 11 0.0 4 10 1 15 2 0.0 4 2 16 6 0.0 4 4 2 17 8 0.0 5 5 2 1 18 3 0.0 5 2 0 1 19 31 0.0 5 31 20 90 0.0 6 85 5 21 66 0.0 6 65 1 22 34 0.0 6 30 4 23 36 0.0 6 31 5 24 22 0.0 6 20 2 25 16 0.0 6 15 1 26 21 0.0 6 20 1 27 27 0.0 6 25 2 28 14 0.0 6 11 3 29 7 0.0 6 7 30 14 0.0 6 13 1 31 23 0.0 6 22 1 32 19 0.0 6 17 1 1 33 11 0.0 6 10 0 0 0 0 1 34 13 0.0 6 9 2 2 35 11 0.0 6 11 36 8 0.0 6 7 0 0 0 0 0 1 37 11 0.0 6 9 2 38 17 0.0 6 15 1 0 0 1 39 12 0.0 6 12 40 18 0.0 6 15 1 1 1 41 6 0.0 6 6 42 13 0.0 6 10 1 2 43 16 0.0 6 15 0 1 44 21 0.0 6 19 2 45 17 0.0 6 14 3 46 11 0.0 6 10 1 47 13 0.0 6 12 1 48 15 0.0 6 15 49 30 0.0 6 27 2 0 1 50 25 0.0 6 22 3 51 25 0.0 6 22 3 52 25 0.0 6 21 2 1 0 1 53 15 0.0 6 12 3 54 10 0.0 6 8 2 55 26 0.0 6 25 1 56 29 0.0 6 26 0 0 0 3 57 27 0.0 6 23 4 58 18 0.0 6 16 2 59 20 0.0 6 19 1 60 28 0.0 6 27 0 0 0 1 61 13 0.0 6 12 0 1 62 16 0.0 6 15 1 63 16 0.0 6 15 1 64 19 0.0 6 17 1 0 0 0 0 1 65 9 0.0 6 9 66 16 0.0 6 14 2 67 25 0.0 6 21 4 68 31 0.0 6 26 4 0 0 1 69 44 0.0 6 40 3 1 70 146 0.0 6 138 7 1 71 43 0.0 6 38 5 72 94 0.0 6 88 6 73 32 0.0 6 28 4 74 39 0.0 6 36 3 75 34 0.0 6 32 2 76 25 0.0 6 20 3 2 77 25 0.0 6 24 1 78 31 0.0 6 30 1 79 20 0.0 6 18 2 80 35 0.0 6 33 2 81 45 0.0 6 40 4 1 82 34 0.0 6 29 4 1 83 45 0.0 6 43 2 84 48 0.0 6 46 2 85 41 0.0 6 36 4 0 0 1 86 54 0.0 6 52 2 87 40 0.0 6 36 3 0 0 1 88 32 0.0 6 29 2 0 0 1 89 36 0.0 6 34 2 90 51 0.0 6 48 2 1 91 35 0.0 6 31 3 1 92 30 0.0 6 29 1 93 51 0.0 6 50 1 94 103 0.0 6 92 10 1 95 38 0.0 6 35 2 0 0 1 96 70 0.0 6 67 3 97 44 0.0 6 38 5 1 98 47 0.0 6 43 3 0 0 0 1 99 77 0.0 6 71 3 3 100 58 0.0 6 55 3 101 70 0.0 6 65 5 102 101 0.0 6 95 4 2 103 91 0.0 6 88 3 104 75 0.0 6 69 6 105 84 0.0 6 78 5 0 0 1 106 73 0.0 6 70 3 107 104 0.0 6 100 3 0 0 0 1 108 77 0.0 6 75 2 109 62 0.0 6 59 3 110 78 0.0 6 73 3 1 0 0 1 111 67 0.0 6 62 5 112 70 0.0 6 63 7 113 70 0.0 6 67 2 1 114 89 0.0 6 83 5 1 115 93 0.0 6 86 7 116 72 0.0 6 70 2 117 70 0.0 6 66 4 118 79 0.0 6 75 4 119 113 0.0 6 109 2 1 0 1 120 127 0.0 6 117 9 0 0 1 121 96 0.0 6 93 3 122 111 0.0 6 107 4 123 121 0.0 6 119 1 0 0 1 124 142 0.0 6 138 4 125 91 0.0 6 87 3 1 126 81 0.0 6 77 4 127 112 0.0 6 107 5 128 146 0.0 6 146 129 129 0.0 6 124 5 130 127 0.0 6 126 1 131 181 0.0 6 178 3 132 161 0.0 6 160 1 133 152 0.0 6 147 4 0 1 134 219 0.0 6 212 5 1 0 1 135 364 0.0 6 349 15 136 298 0.0 6 292 6 137 437 0.0 6 431 6 138 466 0.0 6 459 6 1 139 792 0.0 6 784 8 140 1295 0.0 6 1285 10 141 597 0.0 6 591 6 142 468 0.0 6 449 17 0 0 0 1 1 143 352 0.0 6 346 4 0 0 1 1 144 524 0.0 6 509 14 0 0 1 145 612 0.0 6 606 5 0 0 1 146 487 0.0 6 479 8 147 924 0.0 6 913 10 0 1 148 684 0.0 6 664 17 2 0 0 1 149 499 0.0 6 480 18 0 0 1 150 565 0.0 6 547 16 1 0 1 151 570 0.0 6 560 7 0 0 1 2 152 834 0.0 6 812 22 153 1847 0.0 6 1823 22 1 0 1 154 1410 0.0 6 1394 15 0 1 155 1417 0.0 6 1399 14 2 1 1 156 646 0.0 6 634 12 157 508 0.0 6 489 15 1 0 2 0 1 158 722 0.0 6 700 20 0 0 2 159 471 0.0 6 457 14 160 897 0.0 6 878 19 161 708 0.0 6 697 11 162 933 0.0 6 910 21 1 1 163 835 0.0 6 818 16 1 164 1222 0.0 6 1201 19 1 0 1 165 701 0.0 6 674 26 0 1 166 778 0.0 6 757 20 0 1 167 518 0.0 6 510 7 0 0 1 168 791 0.0 6 772 18 1 169 644 0.0 6 634 9 0 1 170 683 0.0 6 672 8 1 1 0 0 1 171 1724 0.0 6 1705 17 0 0 2 172 553 0.0 6 550 3 173 1045 0.0 6 1025 16 2 1 0 1 174 583 0.0 6 553 28 2 175 1067 0.0 6 1029 36 0 0 2 176 784 0.0 6 748 31 2 0 1 2 177 641 0.0 6 611 26 4 178 616 0.0 6 585 29 1 1 179 743 0.0 6 711 27 2 0 1 1 1 180 637 0.0 6 599 36 0 2 181 970 0.0 6 935 29 3 2 1 182 556 0.0 6 537 18 0 0 1 183 1031 0.0 6 998 31 2 184 330 0.0 6 320 7 3 185 280 0.0 6 260 18 1 0 1 186 1012 0.0 6 966 37 5 2 2 187 1773 0.0 6 1705 63 1 0 2 2 188 1751 0.0 6 1680 69 1 0 0 1 189 1172 0.0 6 1122 47 0 1 1 1 190 1827 0.0 6 1761 59 5 0 1 1 191 1631 0.0 6 1585 42 1 0 3 192 1186 0.0 6 1132 52 0 0 1 1 193 875 0.0 6 836 35 3 0 1 194 1303 0.0 6 1251 46 3 0 2 1 195 1246 0.0 6 1187 53 2 1 3 196 2876 0.0 6 2757 112 3 0 4 197 946 0.0 6 894 50 1 0 1 198 1527 0.0 6 1439 84 2 1 1 199 3395 0.0 6 3269 120 2 0 3 1 200 1075 0.0 6 1023 47 3 1 0 1 201 1714 0.0 6 1643 65 2 0 2 2 202 926 0.0 6 892 31 3 203 659 0.0 6 631 26 2 204 951 0.0 6 919 32 205 367 0.0 6 355 11 0 0 0 1 206 504 0.0 6 486 17 0 0 1 207 292 0.0 6 281 11 208 432 0.0 6 417 12 1 1 1 209 508 0.0 6 493 14 0 0 0 1 210 479 0.0 6 455 23 1 211 707 0.0 6 671 34 1 0 1 212 896 0.0 6 866 29 0 0 1 213 258 0.0 6 247 11 214 649 0.0 6 622 25 1 0 0 1 215 424 0.0 6 407 16 0 0 1 216 562 0.0 6 530 27 2 1 2 217 1101 0.0 6 1070 31 218 499 0.0 6 477 21 1 219 645 0.0 6 625 18 0 1 1 220 356 0.0 6 339 16 1 221 707 0.0 6 681 25 1 222 276 0.0 6 270 6 223 449 0.0 6 440 8 0 1 224 383 0.0 6 367 14 2 225 586 0.0 6 559 25 0 1 1 226 579 0.0 6 554 23 1 1 227 418 0.0 6 400 17 0 0 1 228 227 0.0 6 215 12 229 548 0.0 6 523 25 230 221 0.0 6 215 6 231 330 0.0 6 316 11 2 0 1 232 291 0.0 6 282 9 233 398 0.0 6 383 15 234 178 0.0 6 173 5 235 180 0.0 6 173 7 236 65 0.0 6 58 7 237 97 0.0 6 94 3 238 30 0.0 6 27 3 239 34 0.0 6 30 3 1 240 76 0.0 6 75 1 241 153 0.0 6 149 4 242 217 0.0 6 204 12 1 243 253 0.0 6 240 10 1 0 0 2 244 270 0.0 6 258 12 245 97 0.0 6 96 1 246 84 0.0 6 81 3 247 28 0.0 6 28 248 51 0.0 6 47 4 249 33 0.0 6 33 250 27 0.0 6 27 251 52 0.0 6 48 4 252 8 0.0 6 8 253 15 0.0 6 15 254 48 0.0 6 47 1 255 6 0.0 6 6 256 6 0.0 6 6 257 2 0.0 6 2 258 2 0.0 6 2 259 1 0.0 6 1 260 2 0.0 6 2 261 2 0.0 6 2 262 1 0.0 6 1 264 1 0.0 6 1 278 1 0.0 6 1 280 3 0.0 6 3 281 17 0.0 6 15 2 282 27 0.0 6 25 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1179 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 50 2 0.0 6 2 65 1 0.0 6 1 67 1 0.0 6 1 68 4 0.0 6 4 69 19 0.0 6 19 70 82 0.0 6 82 71 13 0.0 6 13 72 1 0.0 6 1 74 3 0.0 6 3 75 12 0.0 6 12 76 7 0.0 6 7 77 53 0.0 6 53 78 16 0.0 6 16 79 2 0.0 6 1 0 0 0 1 80 1 0.0 6 1 81 4 0.0 6 2 0 0 0 2 82 10 0.0 6 10 83 67 0.0 6 67 84 80 0.0 6 80 85 58 0.0 6 58 88 1 0.0 6 0 0 0 0 1 89 4 0.0 6 4 90 9 0.0 6 9 91 11 0.0 6 11 92 41 0.0 6 41 93 16 0.0 6 16 94 83 0.0 6 83 95 4 0.0 6 3 0 0 0 1 98 1 0.0 6 1 99 2 0.0 6 2 100 23 0.0 6 23 101 129 0.0 6 129 102 16 0.0 6 16 103 84 0.0 6 84 104 1 0.0 6 1 106 1 0.0 6 1 116 10 0.0 6 10 117 55 0.0 6 55 118 11 0.0 6 11 119 23 0.0 6 23 124 2 0.0 6 2 125 3 0.0 6 3 126 1 0.0 6 1 127 8 0.0 6 8 128 44 0.0 6 44 145 1 0.0 6 1 160 1 0.0 6 1 165 1 0.0 6 1 166 6 0.0 6 6 167 7 0.0 6 7 168 23 0.0 6 23 169 1 0.0 6 1 170 6 0.0 6 6 171 8 0.0 6 8 172 1 0.0 6 1 176 2 0.0 6 2 177 2 0.0 6 2 178 14 0.0 6 14 179 71 0.0 6 71 180 1 0.0 6 1 182 4 0.0 6 3 0 0 1 183 1 0.0 6 0 0 0 0 1 185 1 0.0 6 1 186 1 0.0 6 1 190 2 0.0 6 2 191 1 0.0 6 1 217 3 0.0 6 3 246 1 0.0 6 1 248 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_18_9_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_18_236_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_18_9_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_18_236_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.43 s (25 µs/read; 2.43 M reads/minute). === Summary === Total read pairs processed: 138,845 Read 1 with adapter: 138,837 (100.0%) Read 2 with adapter: 138,760 (99.9%) == Read fate breakdown == Pairs that were too short: 47 (0.0%) Pairs discarded as untrimmed: 93 (0.1%) Pairs written (passing filters): 138,705 (99.9%) Total basepairs processed: 58,610,793 bp Read 1: 32,219,293 bp Read 2: 26,391,500 bp Total written (filtered): 9,166,606 bp (15.6%) Read 1: 4,579,284 bp Read 2: 4,587,322 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 133869 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 2169.5 0 1 1 4 6 542.4 1 5 1 5 2 135.6 1 2 6 1 33.9 1 0 0 1 7 3 8.5 2 3 8 2 2.1 2 2 9 3 0.5 2 3 10 2 0.1 3 2 12 1 0.0 3 1 13 1 0.0 3 1 14 1 0.0 4 0 1 15 5 0.0 4 3 1 1 16 5 0.0 4 3 2 17 5 0.0 5 5 18 3 0.0 5 1 0 2 19 6 0.0 5 4 1 0 1 20 329 0.0 6 325 4 21 137 0.0 6 134 3 22 117 0.0 6 113 3 1 23 93 0.0 6 92 1 24 48 0.0 6 47 1 25 59 0.0 6 56 3 26 51 0.0 6 49 2 27 28 0.0 6 28 28 32 0.0 6 32 29 27 0.0 6 27 30 17 0.0 6 17 31 16 0.0 6 14 1 1 32 23 0.0 6 19 4 33 14 0.0 6 13 0 1 34 14 0.0 6 13 1 35 18 0.0 6 18 36 11 0.0 6 11 37 10 0.0 6 10 38 15 0.0 6 13 2 39 34 0.0 6 32 2 40 24 0.0 6 21 2 1 41 17 0.0 6 17 42 8 0.0 6 7 1 43 18 0.0 6 17 1 44 24 0.0 6 22 1 0 0 0 1 45 16 0.0 6 12 2 1 1 46 15 0.0 6 14 1 47 15 0.0 6 14 1 48 10 0.0 6 10 49 14 0.0 6 13 1 50 14 0.0 6 13 1 51 20 0.0 6 19 1 52 13 0.0 6 13 53 17 0.0 6 15 0 0 0 1 0 1 54 22 0.0 6 21 1 55 12 0.0 6 12 56 5 0.0 6 5 57 10 0.0 6 8 2 58 9 0.0 6 7 1 0 0 0 0 1 59 11 0.0 6 10 0 0 0 1 60 5 0.0 6 4 1 61 14 0.0 6 13 1 62 11 0.0 6 11 63 16 0.0 6 15 1 64 13 0.0 6 12 1 65 11 0.0 6 10 1 66 15 0.0 6 14 1 67 18 0.0 6 18 68 15 0.0 6 14 1 69 12 0.0 6 12 70 17 0.0 6 16 1 71 17 0.0 6 16 1 72 16 0.0 6 16 73 14 0.0 6 14 74 16 0.0 6 14 1 1 75 15 0.0 6 15 76 19 0.0 6 19 77 21 0.0 6 19 1 0 0 0 1 78 18 0.0 6 16 2 79 24 0.0 6 24 80 14 0.0 6 13 0 0 0 0 1 81 25 0.0 6 24 1 82 18 0.0 6 18 83 19 0.0 6 19 84 33 0.0 6 32 0 0 0 1 85 27 0.0 6 27 86 20 0.0 6 20 87 26 0.0 6 26 88 21 0.0 6 19 2 89 19 0.0 6 18 1 90 20 0.0 6 19 0 0 0 1 91 17 0.0 6 17 92 14 0.0 6 13 0 0 0 0 0 1 93 26 0.0 6 24 2 94 10 0.0 6 10 95 17 0.0 6 15 2 96 22 0.0 6 21 1 97 18 0.0 6 17 1 98 17 0.0 6 16 1 99 18 0.0 6 16 0 0 1 0 1 100 21 0.0 6 20 1 101 19 0.0 6 19 102 28 0.0 6 28 103 29 0.0 6 29 104 27 0.0 6 25 0 0 0 0 0 2 105 44 0.0 6 42 2 106 40 0.0 6 40 107 33 0.0 6 32 1 108 22 0.0 6 22 109 30 0.0 6 28 1 0 0 1 110 39 0.0 6 39 111 48 0.0 6 47 1 112 47 0.0 6 46 1 113 45 0.0 6 43 1 0 0 0 0 1 114 47 0.0 6 47 115 29 0.0 6 27 2 116 43 0.0 6 42 1 117 47 0.0 6 47 118 47 0.0 6 46 0 0 1 119 48 0.0 6 46 1 0 0 0 1 120 52 0.0 6 51 1 121 44 0.0 6 42 1 1 122 53 0.0 6 50 3 123 47 0.0 6 46 0 1 124 57 0.0 6 54 3 125 56 0.0 6 54 1 1 126 56 0.0 6 55 0 1 127 49 0.0 6 48 1 128 59 0.0 6 57 1 0 0 0 0 1 129 65 0.0 6 64 1 130 65 0.0 6 65 131 74 0.0 6 72 2 132 57 0.0 6 54 3 133 74 0.0 6 74 134 70 0.0 6 70 135 82 0.0 6 81 0 1 136 77 0.0 6 77 137 77 0.0 6 77 138 96 0.0 6 96 139 57 0.0 6 55 2 140 89 0.0 6 87 2 141 120 0.0 6 118 2 142 143 0.0 6 140 1 1 0 0 1 143 334 0.0 6 333 1 144 729 0.0 6 723 5 0 1 145 664 0.0 6 659 4 0 0 0 0 1 146 534 0.0 6 529 4 1 147 672 0.0 6 670 2 148 983 0.0 6 980 3 149 847 0.0 6 844 3 150 1169 0.0 6 1165 3 0 0 0 1 151 899 0.0 6 899 152 1001 0.0 6 994 6 0 0 0 1 153 1413 0.0 6 1409 3 0 0 0 1 154 890 0.0 6 888 2 155 682 0.0 6 681 1 156 880 0.0 6 876 3 0 0 1 157 619 0.0 6 618 1 158 679 0.0 6 674 3 1 0 0 0 1 159 930 0.0 6 928 2 160 722 0.0 6 718 2 0 1 1 161 844 0.0 6 841 3 162 829 0.0 6 827 2 163 1034 0.0 6 1033 1 164 993 0.0 6 990 3 165 869 0.0 6 864 5 166 685 0.0 6 682 3 167 673 0.0 6 670 3 168 727 0.0 6 723 1 1 0 0 0 2 169 611 0.0 6 607 3 0 0 0 0 1 170 734 0.0 6 729 3 0 0 0 1 1 171 838 0.0 6 838 172 915 0.0 6 914 1 173 844 0.0 6 840 2 0 0 2 174 865 0.0 6 861 4 175 989 0.0 6 984 4 0 0 1 176 875 0.0 6 873 2 177 840 0.0 6 839 1 178 674 0.0 6 671 1 0 1 0 1 179 596 0.0 6 595 1 180 765 0.0 6 764 1 181 1490 0.0 6 1482 8 182 862 0.0 6 860 2 183 838 0.0 6 831 6 0 0 0 0 1 184 1010 0.0 6 1003 6 0 0 0 0 1 185 966 0.0 6 963 3 186 863 0.0 6 859 3 1 187 889 0.0 6 886 2 0 0 0 0 1 188 857 0.0 6 856 1 189 983 0.0 6 977 5 0 0 0 1 190 1059 0.0 6 1056 3 191 893 0.0 6 890 3 192 1224 0.0 6 1220 4 193 1668 0.0 6 1660 7 1 194 1409 0.0 6 1402 4 0 0 2 0 1 195 1303 0.0 6 1298 4 0 0 1 196 1669 0.0 6 1662 6 0 1 197 1787 0.0 6 1780 6 1 198 1935 0.0 6 1921 10 1 0 2 1 199 2015 0.0 6 2005 8 0 0 1 1 200 1522 0.0 6 1515 5 1 0 1 201 1309 0.0 6 1304 4 0 1 202 1502 0.0 6 1491 8 1 1 0 1 203 1544 0.0 6 1538 6 204 1636 0.0 6 1628 8 205 1605 0.0 6 1598 7 206 1570 0.0 6 1562 8 207 1860 0.0 6 1850 8 1 1 208 1613 0.0 6 1609 4 209 1824 0.0 6 1811 11 0 0 0 2 210 1478 0.0 6 1469 9 211 1435 0.0 6 1434 1 212 1582 0.0 6 1576 5 0 0 0 1 213 1600 0.0 6 1594 5 0 1 214 1359 0.0 6 1349 8 1 0 0 1 215 1636 0.0 6 1618 16 1 0 0 1 216 2022 0.0 6 2011 10 1 217 1485 0.0 6 1477 7 0 0 0 1 218 1751 0.0 6 1743 7 0 0 0 0 1 219 1702 0.0 6 1691 10 0 1 220 1627 0.0 6 1617 8 0 0 0 0 2 221 1238 0.0 6 1230 5 0 2 0 1 222 1347 0.0 6 1340 6 1 223 1484 0.0 6 1481 3 224 1466 0.0 6 1462 4 225 1521 0.0 6 1511 10 226 1599 0.0 6 1591 7 0 0 1 227 1460 0.0 6 1454 5 0 1 228 1208 0.0 6 1204 4 229 1180 0.0 6 1176 4 230 1126 0.0 6 1117 8 0 0 0 0 1 231 1219 0.0 6 1217 2 232 1079 0.0 6 1077 2 233 1079 0.0 6 1074 4 0 0 0 1 234 1163 0.0 6 1158 4 0 0 1 235 1058 0.0 6 1055 2 1 236 898 0.0 6 896 2 237 842 0.0 6 838 3 0 0 0 1 238 987 0.0 6 982 5 239 908 0.0 6 905 2 0 1 240 786 0.0 6 784 2 241 856 0.0 6 854 2 242 785 0.0 6 783 2 243 838 0.0 6 831 7 244 760 0.0 6 754 3 1 1 1 245 572 0.0 6 566 6 246 623 0.0 6 618 2 0 2 0 1 247 479 0.0 6 476 3 248 390 0.0 6 387 3 249 452 0.0 6 450 2 250 457 0.0 6 455 2 251 437 0.0 6 435 1 0 1 252 397 0.0 6 395 2 253 359 0.0 6 355 3 0 1 254 353 0.0 6 351 2 255 358 0.0 6 355 3 256 336 0.0 6 334 2 257 331 0.0 6 327 3 0 0 0 0 1 258 333 0.0 6 330 3 259 371 0.0 6 368 3 260 365 0.0 6 363 1 0 0 0 0 1 261 294 0.0 6 290 3 0 0 1 262 189 0.0 6 189 263 213 0.0 6 211 2 264 232 0.0 6 230 2 265 240 0.0 6 240 266 215 0.0 6 214 0 0 0 0 0 1 267 184 0.0 6 184 268 196 0.0 6 194 2 269 204 0.0 6 201 3 270 191 0.0 6 189 1 0 0 0 1 271 163 0.0 6 159 3 1 272 147 0.0 6 146 1 273 142 0.0 6 142 274 164 0.0 6 162 2 275 217 0.0 6 216 1 276 225 0.0 6 223 1 1 277 282 0.0 6 280 2 278 380 0.0 6 377 3 279 1404 0.0 6 1393 10 1 280 2348 0.0 6 2339 7 0 0 2 281 12 0.0 6 11 0 1 282 1 0.0 6 1 283 1 0.0 6 1 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 4968 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 54 34 0.0 6 32 2 57 1 0.0 6 0 0 0 0 1 58 1 0.0 6 0 0 0 0 1 60 1 0.0 6 0 1 61 90 0.0 6 7 82 1 62 480 0.0 6 461 19 63 3 0.0 6 3 64 5 0.0 6 5 69 1 0.0 6 1 70 3 0.0 6 3 71 17 0.0 6 17 72 26 0.0 6 26 73 26 0.0 6 26 74 22 0.0 6 21 1 75 23 0.0 6 23 76 44 0.0 6 44 77 37 0.0 6 37 78 49 0.0 6 48 1 79 36 0.0 6 36 80 55 0.0 6 55 81 52 0.0 6 52 82 37 0.0 6 37 83 29 0.0 6 28 1 84 33 0.0 6 32 1 85 25 0.0 6 25 86 19 0.0 6 19 87 29 0.0 6 29 88 17 0.0 6 17 89 30 0.0 6 30 90 23 0.0 6 21 2 91 33 0.0 6 33 92 37 0.0 6 37 93 27 0.0 6 27 94 23 0.0 6 22 1 95 48 0.0 6 48 96 19 0.0 6 19 97 21 0.0 6 21 98 29 0.0 6 29 99 29 0.0 6 29 100 36 0.0 6 36 101 16 0.0 6 16 102 58 0.0 6 58 103 48 0.0 6 48 104 16 0.0 6 16 105 32 0.0 6 31 1 106 21 0.0 6 21 107 17 0.0 6 17 108 31 0.0 6 31 109 68 0.0 6 68 110 17 0.0 6 17 111 26 0.0 6 25 1 112 30 0.0 6 30 113 35 0.0 6 35 114 16 0.0 6 16 115 18 0.0 6 18 116 24 0.0 6 24 117 18 0.0 6 18 118 20 0.0 6 20 119 18 0.0 6 18 120 34 0.0 6 34 121 38 0.0 6 37 1 122 38 0.0 6 38 123 21 0.0 6 21 124 30 0.0 6 30 125 24 0.0 6 24 126 29 0.0 6 29 127 36 0.0 6 36 128 20 0.0 6 20 129 21 0.0 6 21 130 29 0.0 6 28 1 131 48 0.0 6 48 132 39 0.0 6 39 133 38 0.0 6 38 134 36 0.0 6 36 135 30 0.0 6 29 1 136 34 0.0 6 34 137 32 0.0 6 32 138 43 0.0 6 43 139 36 0.0 6 36 140 25 0.0 6 25 141 24 0.0 6 24 142 15 0.0 6 15 143 20 0.0 6 20 144 31 0.0 6 31 145 17 0.0 6 17 146 21 0.0 6 21 147 35 0.0 6 35 148 37 0.0 6 37 149 34 0.0 6 33 0 0 0 0 0 1 150 36 0.0 6 35 0 0 0 0 0 1 151 30 0.0 6 30 152 30 0.0 6 30 153 37 0.0 6 37 154 29 0.0 6 29 155 28 0.0 6 28 156 70 0.0 6 68 2 157 39 0.0 6 39 158 21 0.0 6 21 159 40 0.0 6 40 160 19 0.0 6 19 161 31 0.0 6 31 162 47 0.0 6 47 163 36 0.0 6 36 164 25 0.0 6 25 165 26 0.0 6 26 166 33 0.0 6 33 167 43 0.0 6 43 168 20 0.0 6 20 169 34 0.0 6 34 170 31 0.0 6 31 171 43 0.0 6 42 1 172 39 0.0 6 39 173 20 0.0 6 20 174 34 0.0 6 34 175 24 0.0 6 24 176 18 0.0 6 18 177 9 0.0 6 9 178 18 0.0 6 18 179 31 0.0 6 31 180 12 0.0 6 12 181 20 0.0 6 20 182 13 0.0 6 13 183 27 0.0 6 26 1 184 42 0.0 6 42 185 36 0.0 6 36 186 16 0.0 6 16 187 33 0.0 6 33 188 25 0.0 6 25 189 17 0.0 6 17 190 19 0.0 6 19 191 32 0.0 6 32 192 36 0.0 6 36 193 17 0.0 6 17 194 12 0.0 6 12 195 13 0.0 6 13 196 15 0.0 6 15 197 17 0.0 6 17 198 23 0.0 6 23 199 12 0.0 6 12 200 18 0.0 6 18 201 17 0.0 6 17 202 18 0.0 6 18 203 24 0.0 6 24 204 11 0.0 6 11 205 26 0.0 6 26 206 6 0.0 6 6 207 13 0.0 6 13 208 18 0.0 6 18 209 10 0.0 6 10 210 10 0.0 6 10 211 29 0.0 6 29 212 16 0.0 6 16 213 5 0.0 6 5 214 17 0.0 6 17 215 18 0.0 6 18 216 15 0.0 6 15 217 15 0.0 6 15 218 13 0.0 6 12 1 219 10 0.0 6 10 220 12 0.0 6 12 221 11 0.0 6 11 222 6 0.0 6 6 223 9 0.0 6 9 224 8 0.0 6 8 225 12 0.0 6 12 226 10 0.0 6 10 227 10 0.0 6 10 228 9 0.0 6 9 229 14 0.0 6 14 230 11 0.0 6 11 231 7 0.0 6 7 232 7 0.0 6 7 233 1 0.0 6 1 234 7 0.0 6 7 235 11 0.0 6 11 236 5 0.0 6 5 237 4 0.0 6 4 238 1 0.0 6 1 239 6 0.0 6 6 240 4 0.0 6 4 241 7 0.0 6 7 242 3 0.0 6 3 243 4 0.0 6 4 244 3 0.0 6 3 245 3 0.0 6 3 246 4 0.0 6 4 247 7 0.0 6 7 248 5 0.0 6 5 249 6 0.0 6 6 250 5 0.0 6 5 251 7 0.0 6 7 252 8 0.0 6 8 253 6 0.0 6 6 254 2 0.0 6 2 255 1 0.0 6 1 256 1 0.0 6 1 257 3 0.0 6 3 259 4 0.0 6 4 260 1 0.0 6 1 261 4 0.0 6 4 262 2 0.0 6 2 264 4 0.0 6 4 265 3 0.0 6 2 1 266 3 0.0 6 3 267 1 0.0 6 1 269 2 0.0 6 2 270 2 0.0 6 2 271 2 0.0 6 2 272 4 0.0 6 4 273 1 0.0 6 1 275 1 0.0 6 1 276 2 0.0 6 2 277 1 0.0 6 1 278 1 0.0 6 1 279 2 0.0 6 2 280 1 0.0 6 1 283 1 0.0 6 1 285 2 0.0 6 2 287 1 0.0 6 1 291 1 0.0 6 1 298 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 134275 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 2169.5 0 2 2 6 2 33.9 1 2 7 1 8.5 2 0 1 9 2 0.5 2 1 0 1 10 5 0.1 3 5 11 6 0.0 3 6 12 2 0.0 3 2 13 17 0.0 3 14 2 0 1 14 12 0.0 4 9 3 15 3 0.0 4 1 2 16 29 0.0 4 24 5 17 40 0.0 5 35 4 0 1 18 45 0.0 5 44 1 19 37 0.0 5 33 2 2 20 251 0.0 6 243 7 1 21 129 0.0 6 127 2 22 67 0.0 6 65 2 23 58 0.0 6 56 0 1 0 1 24 54 0.0 6 50 4 25 48 0.0 6 46 2 26 34 0.0 6 33 1 27 19 0.0 6 16 2 0 0 1 28 37 0.0 6 36 1 29 34 0.0 6 30 4 30 32 0.0 6 31 0 1 31 31 0.0 6 28 3 32 19 0.0 6 17 2 33 21 0.0 6 17 3 0 0 0 0 1 34 22 0.0 6 21 1 35 23 0.0 6 19 2 1 0 1 36 27 0.0 6 24 3 37 23 0.0 6 21 1 1 38 17 0.0 6 17 39 18 0.0 6 13 4 0 1 40 32 0.0 6 29 3 41 18 0.0 6 17 0 1 42 27 0.0 6 23 2 1 0 1 43 30 0.0 6 26 2 0 0 0 0 2 44 20 0.0 6 19 1 45 22 0.0 6 21 1 46 28 0.0 6 27 1 47 28 0.0 6 24 4 48 28 0.0 6 26 2 49 36 0.0 6 32 4 50 31 0.0 6 26 5 51 38 0.0 6 36 2 52 28 0.0 6 24 2 2 53 40 0.0 6 39 1 54 28 0.0 6 26 2 55 41 0.0 6 37 3 1 56 44 0.0 6 38 6 57 45 0.0 6 39 6 58 42 0.0 6 38 4 59 37 0.0 6 36 0 0 1 60 33 0.0 6 30 2 1 61 34 0.0 6 30 4 62 40 0.0 6 36 4 63 55 0.0 6 47 6 1 0 0 1 64 63 0.0 6 56 4 1 1 0 0 1 65 60 0.0 6 53 6 1 66 71 0.0 6 68 3 67 253 0.0 6 243 6 0 2 0 1 1 68 101 0.0 6 88 11 1 0 1 69 140 0.0 6 131 7 0 0 0 1 1 70 62 0.0 6 58 3 0 0 0 0 1 71 93 0.0 6 85 7 0 0 0 0 1 72 58 0.0 6 55 2 0 0 0 0 1 73 59 0.0 6 54 3 1 1 74 69 0.0 6 64 3 0 0 0 1 1 75 73 0.0 6 68 5 76 64 0.0 6 61 2 0 1 77 62 0.0 6 55 7 78 72 0.0 6 62 8 1 1 79 81 0.0 6 76 3 0 0 1 0 1 80 77 0.0 6 74 3 81 79 0.0 6 74 3 2 82 91 0.0 6 89 2 83 122 0.0 6 115 5 2 84 80 0.0 6 77 2 1 85 89 0.0 6 83 4 2 86 81 0.0 6 78 3 87 81 0.0 6 76 3 1 1 88 74 0.0 6 69 5 89 85 0.0 6 81 3 0 0 0 0 1 90 83 0.0 6 79 4 91 109 0.0 6 102 6 0 0 1 92 107 0.0 6 102 5 93 136 0.0 6 131 4 1 94 110 0.0 6 104 6 95 164 0.0 6 154 9 1 96 137 0.0 6 129 6 1 0 0 0 1 97 136 0.0 6 126 8 0 0 0 1 1 98 114 0.0 6 110 2 1 1 99 119 0.0 6 116 3 100 123 0.0 6 121 2 101 142 0.0 6 129 11 1 0 0 0 1 102 132 0.0 6 120 10 2 103 176 0.0 6 173 2 0 0 0 0 1 104 303 0.0 6 291 12 105 171 0.0 6 168 2 0 0 0 1 106 160 0.0 6 152 7 1 107 149 0.0 6 145 2 1 0 1 108 168 0.0 6 164 3 1 109 166 0.0 6 163 2 1 110 199 0.0 6 192 6 0 0 1 111 206 0.0 6 203 2 0 0 0 0 1 112 252 0.0 6 245 6 1 113 236 0.0 6 229 5 0 0 1 1 114 237 0.0 6 229 8 115 282 0.0 6 272 10 116 248 0.0 6 244 3 0 1 117 268 0.0 6 260 8 118 313 0.0 6 306 7 119 262 0.0 6 259 2 0 0 0 1 120 287 0.0 6 278 9 121 293 0.0 6 286 7 122 327 0.0 6 316 11 123 345 0.0 6 336 9 124 387 0.0 6 381 4 0 1 0 0 1 125 440 0.0 6 432 7 1 126 451 0.0 6 441 9 0 0 0 1 127 496 0.0 6 483 12 0 0 0 0 1 128 553 0.0 6 535 16 0 2 129 639 0.0 6 574 57 2 1 1 2 2 130 907 0.0 6 870 36 0 0 0 1 131 753 0.0 6 740 12 1 132 695 0.0 6 679 15 0 1 133 767 0.0 6 753 13 0 1 134 829 0.0 6 815 13 0 0 0 1 135 785 0.0 6 766 14 0 2 2 0 1 136 766 0.0 6 749 15 0 0 1 0 1 137 864 0.0 6 845 17 0 0 1 0 1 138 811 0.0 6 799 10 0 0 1 0 1 139 1141 0.0 6 1118 22 0 0 1 140 1479 0.0 6 1462 9 1 1 1 2 3 141 1544 0.0 6 1529 11 1 0 2 1 142 1671 0.0 6 1654 14 1 0 1 0 1 143 1596 0.0 6 1580 13 0 1 0 1 1 144 1933 0.0 6 1917 14 0 0 0 1 1 145 1844 0.0 6 1821 20 0 3 146 2215 0.0 6 2187 19 4 1 1 2 1 147 2022 0.0 6 1993 22 2 2 0 1 2 148 2531 0.0 6 2500 27 0 1 0 1 2 149 2770 0.0 6 2735 30 2 0 1 1 1 150 3214 0.0 6 3169 39 1 0 2 2 1 151 2380 0.0 6 2351 24 2 0 1 1 1 152 2886 0.0 6 2839 38 6 1 0 1 1 153 2620 0.0 6 2590 26 2 0 2 154 2309 0.0 6 2271 34 1 2 0 0 1 155 2558 0.0 6 2530 22 1 1 1 2 1 156 2184 0.0 6 2163 15 1 3 1 0 1 157 2423 0.0 6 2394 23 3 1 0 1 1 158 2557 0.0 6 2524 30 0 2 1 159 2275 0.0 6 2254 16 3 2 160 2372 0.0 6 2349 18 2 0 0 0 3 161 2179 0.0 6 2170 6 1 0 1 1 162 1833 0.0 6 1803 25 0 2 0 2 1 163 1912 0.0 6 1892 19 0 0 0 1 164 1864 0.0 6 1847 14 0 3 165 1727 0.0 6 1707 16 0 0 2 2 166 2336 0.0 6 2310 20 1 2 0 2 1 167 1980 0.0 6 1955 21 2 0 2 168 2433 0.0 6 2410 21 1 0 0 1 169 2086 0.0 6 2065 17 1 1 1 0 1 170 2082 0.0 6 2061 14 1 1 2 0 3 171 2070 0.0 6 2049 17 1 1 0 0 2 172 2227 0.0 6 2211 12 0 1 2 0 1 173 2074 0.0 6 2057 14 1 0 0 2 174 1901 0.0 6 1879 18 1 0 0 0 3 175 1930 0.0 6 1910 17 0 1 1 0 1 176 2120 0.0 6 2107 11 1 1 177 1674 0.0 6 1668 5 0 0 1 178 1671 0.0 6 1655 9 0 2 1 2 2 179 1363 0.0 6 1352 9 0 1 0 0 1 180 1225 0.0 6 1219 5 1 181 1306 0.0 6 1300 6 182 1197 0.0 6 1194 1 1 0 0 1 183 1696 0.0 6 1678 15 1 0 1 0 1 184 2051 0.0 6 2039 10 0 0 0 0 2 185 1858 0.0 6 1846 10 1 0 1 186 1389 0.0 6 1378 10 0 0 0 1 187 1292 0.0 6 1283 7 0 0 2 188 1184 0.0 6 1174 9 0 0 1 189 1159 0.0 6 1154 5 190 1032 0.0 6 1028 3 0 0 0 1 191 966 0.0 6 958 6 0 0 1 0 1 192 846 0.0 6 836 7 0 0 1 0 2 193 712 0.0 6 705 7 194 685 0.0 6 683 0 1 0 0 1 195 618 0.0 6 615 3 196 762 0.0 6 757 2 0 1 2 197 614 0.0 6 608 5 0 0 0 1 198 594 0.0 6 593 1 199 540 0.0 6 538 1 0 0 0 1 200 437 0.0 6 435 2 201 395 0.0 6 392 3 202 415 0.0 6 409 6 203 379 0.0 6 371 6 0 2 204 336 0.0 6 334 2 205 270 0.0 6 270 206 254 0.0 6 254 207 294 0.0 6 292 2 208 248 0.0 6 245 3 209 212 0.0 6 211 1 210 155 0.0 6 154 1 211 174 0.0 6 170 3 1 212 186 0.0 6 185 1 213 148 0.0 6 146 1 0 0 0 1 214 201 0.0 6 200 1 215 173 0.0 6 173 216 135 0.0 6 134 1 217 123 0.0 6 122 1 218 104 0.0 6 104 219 63 0.0 6 62 1 220 74 0.0 6 74 221 94 0.0 6 93 1 222 68 0.0 6 68 223 69 0.0 6 66 3 224 59 0.0 6 59 225 42 0.0 6 42 226 50 0.0 6 50 227 36 0.0 6 36 228 34 0.0 6 34 229 19 0.0 6 19 230 12 0.0 6 12 231 26 0.0 6 26 232 22 0.0 6 22 233 11 0.0 6 11 234 7 0.0 6 7 235 7 0.0 6 7 236 6 0.0 6 6 237 6 0.0 6 6 238 4 0.0 6 4 239 6 0.0 6 6 240 3 0.0 6 3 241 15 0.0 6 15 242 9 0.0 6 9 243 6 0.0 6 6 244 2 0.0 6 2 245 4 0.0 6 4 246 2 0.0 6 2 247 4 0.0 6 4 248 3 0.0 6 3 250 3 0.0 6 3 251 1 0.0 6 1 276 1 0.0 6 1 277 2 0.0 6 2 278 15 0.0 6 15 279 120 0.0 6 118 2 280 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 4485 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 53 1 0.0 6 1 58 1 0.0 6 1 60 4 0.0 6 4 62 2 0.0 6 2 63 1 0.0 6 1 71 4 0.0 6 4 72 19 0.0 6 19 73 56 0.0 6 56 74 30 0.0 6 30 75 22 0.0 6 22 76 33 0.0 6 33 77 41 0.0 6 40 1 78 39 0.0 6 39 79 46 0.0 6 46 80 49 0.0 6 48 0 0 1 81 54 0.0 6 54 82 70 0.0 6 70 83 32 0.0 6 32 84 32 0.0 6 32 85 38 0.0 6 37 0 0 0 1 86 39 0.0 6 38 0 0 0 1 87 77 0.0 6 75 2 88 52 0.0 6 52 89 36 0.0 6 35 0 0 0 1 90 33 0.0 6 33 91 42 0.0 6 41 1 92 69 0.0 6 68 0 0 0 1 93 44 0.0 6 44 94 52 0.0 6 52 95 20 0.0 6 19 1 96 17 0.0 6 17 97 53 0.0 6 53 98 11 0.0 6 11 99 32 0.0 6 31 0 0 0 1 100 42 0.0 6 42 101 34 0.0 6 34 102 20 0.0 6 20 103 31 0.0 6 31 104 146 0.0 6 146 105 24 0.0 6 24 106 23 0.0 6 23 107 32 0.0 6 32 108 16 0.0 6 16 109 40 0.0 6 40 110 64 0.0 6 64 111 22 0.0 6 22 112 37 0.0 6 37 113 33 0.0 6 33 114 30 0.0 6 30 115 27 0.0 6 27 116 13 0.0 6 13 117 15 0.0 6 14 0 0 0 1 118 42 0.0 6 42 119 24 0.0 6 23 0 0 0 1 120 26 0.0 6 25 0 0 0 1 121 28 0.0 6 28 122 165 0.0 6 163 2 123 39 0.0 6 39 124 39 0.0 6 38 1 125 38 0.0 6 37 1 126 44 0.0 6 44 127 42 0.0 6 42 128 45 0.0 6 45 129 14 0.0 6 13 0 0 0 1 130 37 0.0 6 37 131 27 0.0 6 27 132 53 0.0 6 53 133 35 0.0 6 35 134 46 0.0 6 46 135 30 0.0 6 30 136 24 0.0 6 24 137 35 0.0 6 34 1 138 34 0.0 6 34 139 49 0.0 6 49 140 46 0.0 6 46 141 32 0.0 6 32 142 36 0.0 6 36 143 26 0.0 6 25 1 144 58 0.0 6 57 0 0 1 145 36 0.0 6 35 1 146 16 0.0 6 16 147 26 0.0 6 26 148 88 0.0 6 86 2 149 44 0.0 6 44 150 28 0.0 6 28 151 24 0.0 6 24 152 26 0.0 6 25 0 0 0 1 153 26 0.0 6 25 0 0 0 1 154 48 0.0 6 48 155 21 0.0 6 21 156 34 0.0 6 34 157 38 0.0 6 38 158 53 0.0 6 53 159 31 0.0 6 31 160 31 0.0 6 31 161 23 0.0 6 23 162 23 0.0 6 22 0 0 0 1 163 44 0.0 6 44 164 34 0.0 6 34 165 27 0.0 6 27 166 24 0.0 6 24 167 45 0.0 6 45 168 44 0.0 6 44 169 21 0.0 6 21 170 37 0.0 6 36 0 1 171 42 0.0 6 41 1 172 45 0.0 6 45 173 33 0.0 6 32 1 174 23 0.0 6 22 0 0 0 1 175 28 0.0 6 28 176 17 0.0 6 17 177 15 0.0 6 14 1 178 12 0.0 6 12 179 13 0.0 6 13 180 24 0.0 6 24 181 9 0.0 6 9 182 21 0.0 6 21 183 3 0.0 6 2 0 0 0 1 184 12 0.0 6 12 185 31 0.0 6 31 186 27 0.0 6 27 187 15 0.0 6 15 188 42 0.0 6 42 189 14 0.0 6 14 190 5 0.0 6 3 1 0 0 1 191 11 0.0 6 11 192 16 0.0 6 16 193 17 0.0 6 17 194 15 0.0 6 15 195 4 0.0 6 4 196 6 0.0 6 6 197 10 0.0 6 10 198 10 0.0 6 10 199 9 0.0 6 9 200 10 0.0 6 10 201 12 0.0 6 12 202 5 0.0 6 5 203 8 0.0 6 8 204 5 0.0 6 5 205 7 0.0 6 7 206 4 0.0 6 4 207 6 0.0 6 5 0 0 0 1 208 2 0.0 6 2 209 5 0.0 6 5 210 2 0.0 6 2 211 4 0.0 6 4 212 10 0.0 6 10 213 6 0.0 6 6 214 7 0.0 6 7 215 3 0.0 6 3 216 5 0.0 6 5 217 3 0.0 6 3 218 3 0.0 6 3 219 5 0.0 6 5 220 1 0.0 6 1 221 2 0.0 6 2 222 1 0.0 6 1 223 1 0.0 6 1 225 1 0.0 6 1 226 1 0.0 6 1 227 2 0.0 6 2 228 2 0.0 6 2 229 2 0.0 6 2 230 4 0.0 6 4 231 3 0.0 6 3 232 3 0.0 6 3 234 1 0.0 6 1 235 2 0.0 6 2 236 1 0.0 6 1 238 1 0.0 6 1 240 2 0.0 6 1 0 0 1 241 2 0.0 6 2 244 1 0.0 6 1 247 1 0.0 6 1 248 1 0.0 6 1 250 1 0.0 6 1 255 1 0.0 6 0 0 1 262 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_19_10_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_19_237_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_19_10_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_19_237_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.09 s (25 µs/read; 2.42 M reads/minute). === Summary === Total read pairs processed: 84,239 Read 1 with adapter: 84,236 (100.0%) Read 2 with adapter: 84,207 (100.0%) == Read fate breakdown == Pairs that were too short: 1 (0.0%) Pairs discarded as untrimmed: 35 (0.0%) Pairs written (passing filters): 84,203 (100.0%) Total basepairs processed: 43,325,363 bp Read 1: 24,379,101 bp Read 2: 18,946,262 bp Total written (filtered): 5,140,100 bp (11.9%) Read 1: 2,612,465 bp Read 2: 2,527,635 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 84193 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 82.3 1 1 6 3 20.6 1 0 0 3 7 1 5.1 2 1 14 2 0.0 4 2 15 1 0.0 4 1 16 2 0.0 4 2 20 19 0.0 6 19 21 6 0.0 6 4 2 22 7 0.0 6 7 23 1 0.0 6 1 24 2 0.0 6 1 1 25 17 0.0 6 16 1 26 5 0.0 6 5 27 3 0.0 6 2 1 28 4 0.0 6 2 2 29 3 0.0 6 2 1 30 4 0.0 6 4 31 2 0.0 6 2 32 2 0.0 6 2 33 2 0.0 6 2 34 3 0.0 6 3 35 3 0.0 6 3 36 1 0.0 6 1 37 1 0.0 6 1 38 3 0.0 6 2 0 1 39 6 0.0 6 6 40 1 0.0 6 1 41 4 0.0 6 3 1 42 3 0.0 6 3 43 1 0.0 6 1 44 4 0.0 6 4 45 5 0.0 6 5 46 2 0.0 6 2 47 2 0.0 6 2 48 1 0.0 6 1 49 2 0.0 6 2 50 4 0.0 6 4 51 1 0.0 6 1 52 1 0.0 6 1 53 3 0.0 6 3 54 6 0.0 6 5 1 55 1 0.0 6 1 57 7 0.0 6 7 59 2 0.0 6 2 61 1 0.0 6 1 62 5 0.0 6 5 63 1 0.0 6 1 64 1 0.0 6 1 65 3 0.0 6 3 66 3 0.0 6 2 1 67 2 0.0 6 2 68 1 0.0 6 0 1 69 1 0.0 6 1 70 1 0.0 6 0 0 1 71 1 0.0 6 1 74 2 0.0 6 2 75 1 0.0 6 1 76 2 0.0 6 2 77 4 0.0 6 4 78 5 0.0 6 5 79 2 0.0 6 2 80 1 0.0 6 1 81 1 0.0 6 1 82 3 0.0 6 3 83 1 0.0 6 1 84 1 0.0 6 1 85 1 0.0 6 1 86 7 0.0 6 7 87 6 0.0 6 6 89 3 0.0 6 3 90 1 0.0 6 1 91 1 0.0 6 1 92 6 0.0 6 6 97 7 0.0 6 7 98 7 0.0 6 7 99 1 0.0 6 1 100 9 0.0 6 9 101 13 0.0 6 12 1 102 8 0.0 6 7 1 103 7 0.0 6 7 104 3 0.0 6 3 105 1 0.0 6 1 106 1 0.0 6 1 107 10 0.0 6 10 108 13 0.0 6 13 109 5 0.0 6 5 110 1 0.0 6 1 111 4 0.0 6 4 112 19 0.0 6 17 2 113 8 0.0 6 6 2 114 12 0.0 6 12 115 8 0.0 6 8 116 4 0.0 6 4 117 4 0.0 6 4 118 2 0.0 6 2 119 3 0.0 6 3 120 6 0.0 6 5 1 121 5 0.0 6 3 1 1 122 5 0.0 6 5 123 13 0.0 6 13 124 7 0.0 6 7 125 8 0.0 6 8 126 6 0.0 6 6 127 4 0.0 6 4 128 19 0.0 6 19 129 9 0.0 6 9 130 12 0.0 6 12 131 10 0.0 6 10 132 23 0.0 6 21 2 133 18 0.0 6 17 1 134 33 0.0 6 31 2 135 34 0.0 6 30 4 136 23 0.0 6 22 1 137 31 0.0 6 30 1 138 20 0.0 6 19 1 139 13 0.0 6 12 1 140 14 0.0 6 14 141 17 0.0 6 16 1 142 24 0.0 6 23 1 143 47 0.0 6 47 144 59 0.0 6 57 2 145 39 0.0 6 39 146 47 0.0 6 46 1 147 44 0.0 6 44 148 46 0.0 6 46 149 61 0.0 6 61 150 57 0.0 6 57 151 66 0.0 6 65 0 1 152 68 0.0 6 66 2 153 35 0.0 6 35 154 67 0.0 6 67 155 31 0.0 6 30 0 1 156 45 0.0 6 45 157 22 0.0 6 22 158 37 0.0 6 37 159 14 0.0 6 14 160 54 0.0 6 53 1 161 38 0.0 6 38 162 46 0.0 6 46 163 47 0.0 6 46 1 164 44 0.0 6 40 4 165 39 0.0 6 38 0 1 166 22 0.0 6 21 1 167 37 0.0 6 35 2 168 51 0.0 6 51 169 50 0.0 6 48 2 170 68 0.0 6 64 4 171 67 0.0 6 66 1 172 30 0.0 6 30 173 51 0.0 6 50 1 174 56 0.0 6 56 175 27 0.0 6 27 176 38 0.0 6 38 177 36 0.0 6 36 178 33 0.0 6 33 179 52 0.0 6 51 0 1 180 48 0.0 6 47 1 181 37 0.0 6 37 182 64 0.0 6 63 0 0 0 1 183 93 0.0 6 92 1 184 45 0.0 6 43 2 185 49 0.0 6 49 186 47 0.0 6 44 2 1 187 59 0.0 6 58 1 188 69 0.0 6 68 1 189 46 0.0 6 43 3 190 30 0.0 6 30 191 51 0.0 6 50 0 1 192 79 0.0 6 77 2 193 112 0.0 6 111 1 194 145 0.0 6 142 3 195 124 0.0 6 120 4 196 118 0.0 6 115 2 1 197 137 0.0 6 132 5 198 73 0.0 6 72 1 199 130 0.0 6 127 3 200 62 0.0 6 60 2 201 53 0.0 6 53 202 59 0.0 6 58 1 203 89 0.0 6 86 3 204 157 0.0 6 154 3 205 127 0.0 6 125 2 206 148 0.0 6 142 5 1 207 165 0.0 6 157 7 1 208 252 0.0 6 248 4 209 341 0.0 6 338 2 1 210 366 0.0 6 349 15 2 211 605 0.0 6 593 12 212 313 0.0 6 304 9 213 284 0.0 6 277 7 214 660 0.0 6 642 18 215 131 0.0 6 126 5 216 652 0.0 6 639 13 217 151 0.0 6 146 5 218 227 0.0 6 221 5 0 0 0 1 219 180 0.0 6 178 2 220 286 0.0 6 281 5 221 122 0.0 6 117 5 222 289 0.0 6 281 8 223 179 0.0 6 177 2 224 528 0.0 6 522 6 225 193 0.0 6 184 9 226 1169 0.0 6 1146 23 227 559 0.0 6 545 13 1 228 427 0.0 6 416 10 0 0 0 1 229 570 0.0 6 559 11 230 344 0.0 6 338 6 231 281 0.0 6 269 12 232 229 0.0 6 216 12 1 233 196 0.0 6 187 9 234 221 0.0 6 217 4 235 145 0.0 6 141 4 236 168 0.0 6 163 2 1 1 1 237 252 0.0 6 246 5 1 238 181 0.0 6 176 5 239 307 0.0 6 294 13 240 1037 0.0 6 1027 10 241 164 0.0 6 158 6 242 221 0.0 6 215 5 1 243 159 0.0 6 151 8 244 177 0.0 6 176 1 245 174 0.0 6 168 6 246 290 0.0 6 274 16 247 746 0.0 6 735 11 248 273 0.0 6 268 4 0 1 249 276 0.0 6 270 6 250 699 0.0 6 682 17 251 522 0.0 6 499 22 1 252 2018 0.0 6 1991 27 253 610 0.0 6 602 8 254 385 0.0 6 369 16 255 696 0.0 6 683 13 256 824 0.0 6 797 25 1 1 257 1448 0.0 6 1417 31 258 1214 0.0 6 1171 40 1 2 259 2551 0.0 6 2505 45 1 260 1027 0.0 6 987 37 3 261 2335 0.0 6 2297 38 262 934 0.0 6 905 29 263 2048 0.0 6 2010 36 2 264 641 0.0 6 629 11 1 265 461 0.0 6 452 8 1 266 1036 0.0 6 1016 20 267 1236 0.0 6 1220 16 268 687 0.0 6 664 22 1 269 1251 0.0 6 1220 30 1 270 882 0.0 6 851 30 1 271 888 0.0 6 871 17 272 368 0.0 6 354 14 273 513 0.0 6 504 9 274 502 0.0 6 485 15 1 0 1 275 847 0.0 6 827 20 276 2906 0.0 6 2846 59 1 277 2263 0.0 6 2198 62 3 278 2840 0.0 6 2739 98 3 279 24301 0.0 6 23863 417 18 2 1 280 6073 0.0 6 5884 185 3 1 281 43 0.0 6 35 5 2 1 282 2 0.0 6 2 283 2 0.0 6 2 284 1 0.0 6 0 0 0 0 0 1 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 43 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 73 1 0.0 6 1 74 1 0.0 6 1 75 1 0.0 6 1 76 2 0.0 6 2 77 1 0.0 6 1 78 1 0.0 6 1 87 1 0.0 6 1 88 2 0.0 6 2 92 1 0.0 6 1 94 2 0.0 6 2 97 1 0.0 6 1 99 2 0.0 6 2 101 1 0.0 6 1 102 1 0.0 6 1 111 1 0.0 6 1 116 1 0.0 6 1 117 1 0.0 6 1 118 1 0.0 6 1 123 2 0.0 6 1 1 124 1 0.0 6 1 127 1 0.0 6 1 137 1 0.0 6 1 147 1 0.0 6 1 151 1 0.0 6 1 153 1 0.0 6 1 155 1 0.0 6 1 160 1 0.0 6 1 166 1 0.0 6 1 167 1 0.0 6 1 170 1 0.0 6 1 171 1 0.0 6 1 172 1 0.0 6 1 174 1 0.0 6 1 178 1 0.0 6 1 183 1 0.0 6 1 188 1 0.0 6 1 203 1 0.0 6 1 208 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 84158 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 2 20.6 1 2 11 1 0.0 3 1 12 2 0.0 3 2 13 7 0.0 3 7 14 5 0.0 4 5 15 1 0.0 4 1 16 6 0.0 4 6 17 5 0.0 5 4 1 18 5 0.0 5 4 1 19 13 0.0 5 11 2 20 30 0.0 6 28 2 21 32 0.0 6 30 2 22 41 0.0 6 39 2 23 31 0.0 6 26 5 24 16 0.0 6 16 25 11 0.0 6 11 26 12 0.0 6 11 0 0 0 1 27 8 0.0 6 8 28 7 0.0 6 7 29 6 0.0 6 6 30 12 0.0 6 10 2 31 5 0.0 6 4 0 1 32 6 0.0 6 4 2 33 6 0.0 6 5 1 34 3 0.0 6 3 35 6 0.0 6 6 36 3 0.0 6 3 37 13 0.0 6 10 2 1 38 6 0.0 6 5 1 39 3 0.0 6 3 40 8 0.0 6 7 1 41 8 0.0 6 7 1 42 5 0.0 6 4 1 43 11 0.0 6 10 1 44 17 0.0 6 17 45 9 0.0 6 7 2 46 17 0.0 6 17 47 17 0.0 6 17 48 8 0.0 6 7 1 49 20 0.0 6 17 3 50 16 0.0 6 16 51 7 0.0 6 7 52 8 0.0 6 8 53 4 0.0 6 4 54 19 0.0 6 19 55 21 0.0 6 21 56 18 0.0 6 16 2 57 13 0.0 6 13 58 9 0.0 6 8 1 59 18 0.0 6 15 1 1 0 0 1 60 17 0.0 6 15 2 61 10 0.0 6 8 2 62 10 0.0 6 10 63 5 0.0 6 5 64 18 0.0 6 13 4 1 65 10 0.0 6 8 1 1 66 16 0.0 6 14 1 0 0 1 67 16 0.0 6 12 4 68 22 0.0 6 19 1 2 69 123 0.0 6 119 4 70 18 0.0 6 16 1 1 71 79 0.0 6 78 1 72 32 0.0 6 30 2 73 26 0.0 6 23 3 74 15 0.0 6 14 1 75 16 0.0 6 16 76 25 0.0 6 23 2 77 22 0.0 6 20 2 78 29 0.0 6 28 1 79 21 0.0 6 20 0 1 80 11 0.0 6 11 81 17 0.0 6 17 82 32 0.0 6 31 1 83 33 0.0 6 31 2 84 38 0.0 6 38 85 49 0.0 6 47 1 0 0 1 86 21 0.0 6 21 87 38 0.0 6 36 2 88 11 0.0 6 10 1 89 21 0.0 6 18 2 1 90 36 0.0 6 33 3 91 45 0.0 6 41 4 92 21 0.0 6 17 3 1 93 53 0.0 6 48 4 1 94 83 0.0 6 76 5 2 95 27 0.0 6 26 1 96 31 0.0 6 30 1 97 30 0.0 6 27 3 98 37 0.0 6 36 1 99 33 0.0 6 30 2 1 100 31 0.0 6 30 1 101 34 0.0 6 30 4 102 62 0.0 6 54 7 0 1 103 75 0.0 6 72 2 1 104 68 0.0 6 65 2 1 105 80 0.0 6 76 4 106 40 0.0 6 35 4 1 107 58 0.0 6 56 1 1 108 58 0.0 6 55 3 109 51 0.0 6 47 2 2 110 40 0.0 6 38 1 1 111 52 0.0 6 51 0 1 112 41 0.0 6 38 3 113 41 0.0 6 37 3 1 114 56 0.0 6 51 4 0 0 0 0 1 115 49 0.0 6 48 1 116 40 0.0 6 38 2 117 34 0.0 6 32 2 118 45 0.0 6 44 1 119 66 0.0 6 58 8 120 87 0.0 6 84 3 121 40 0.0 6 37 2 1 122 62 0.0 6 58 3 1 123 79 0.0 6 74 4 1 124 73 0.0 6 69 3 1 125 44 0.0 6 43 1 126 29 0.0 6 28 1 127 63 0.0 6 58 4 0 0 1 128 58 0.0 6 55 2 1 129 55 0.0 6 55 130 48 0.0 6 44 3 0 0 1 131 106 0.0 6 103 3 132 123 0.0 6 120 2 1 133 56 0.0 6 56 134 133 0.0 6 124 9 135 229 0.0 6 222 7 136 129 0.0 6 121 7 0 0 0 1 137 169 0.0 6 161 8 138 75 0.0 6 72 3 139 176 0.0 6 171 4 1 140 231 0.0 6 222 8 0 0 1 141 219 0.0 6 213 4 1 0 1 142 309 0.0 6 300 8 1 143 291 0.0 6 280 11 144 353 0.0 6 347 5 1 145 250 0.0 6 243 7 146 242 0.0 6 233 9 147 159 0.0 6 154 5 148 229 0.0 6 223 4 1 1 149 346 0.0 6 330 14 0 0 1 1 150 424 0.0 6 408 16 151 316 0.0 6 303 13 152 539 0.0 6 521 17 0 1 153 320 0.0 6 312 8 154 350 0.0 6 341 8 0 0 1 155 234 0.0 6 228 6 156 282 0.0 6 273 8 1 157 388 0.0 6 379 8 1 158 462 0.0 6 444 18 159 203 0.0 6 189 10 2 1 1 160 490 0.0 6 478 10 0 2 161 275 0.0 6 271 3 0 1 162 222 0.0 6 214 7 1 163 439 0.0 6 422 14 1 0 2 164 368 0.0 6 356 12 165 522 0.0 6 503 18 0 0 1 166 660 0.0 6 642 18 167 448 0.0 6 431 15 2 168 733 0.0 6 716 16 1 169 372 0.0 6 363 9 170 498 0.0 6 483 13 1 0 1 171 609 0.0 6 592 16 0 0 1 172 285 0.0 6 272 10 1 1 1 173 394 0.0 6 385 8 1 174 504 0.0 6 487 14 2 0 1 175 775 0.0 6 752 22 0 0 1 176 534 0.0 6 519 15 177 990 0.0 6 963 24 0 1 2 178 620 0.0 6 605 14 1 179 627 0.0 6 611 12 4 180 831 0.0 6 810 17 3 0 0 1 181 657 0.0 6 643 13 0 0 1 182 255 0.0 6 244 11 183 640 0.0 6 622 18 184 261 0.0 6 254 5 0 0 2 185 382 0.0 6 368 13 0 0 1 186 1443 0.0 6 1406 36 0 1 187 1061 0.0 6 1024 36 0 0 1 188 1263 0.0 6 1232 31 189 1344 0.0 6 1306 37 1 190 1742 0.0 6 1686 53 3 191 1681 0.0 6 1629 49 3 192 951 0.0 6 930 19 0 0 2 193 1419 0.0 6 1390 27 1 1 194 917 0.0 6 899 17 0 0 1 195 1106 0.0 6 1065 41 196 1944 0.0 6 1912 32 197 466 0.0 6 447 19 198 2063 0.0 6 2013 46 2 0 2 199 779 0.0 6 753 23 2 0 1 200 1184 0.0 6 1143 38 2 0 1 201 1712 0.0 6 1676 35 0 1 202 1820 0.0 6 1770 45 3 0 2 203 923 0.0 6 903 18 1 0 1 204 1523 0.0 6 1477 43 1 1 1 205 1148 0.0 6 1109 38 0 0 1 206 1089 0.0 6 1057 29 1 0 2 207 1062 0.0 6 1028 34 208 1648 0.0 6 1616 31 0 0 1 209 231 0.0 6 215 13 1 0 1 1 210 711 0.0 6 690 20 0 0 1 211 1548 0.0 6 1499 47 0 0 2 212 931 0.0 6 892 36 1 1 1 213 255 0.0 6 244 8 2 1 214 898 0.0 6 874 24 215 1262 0.0 6 1225 36 0 0 1 216 1860 0.0 6 1804 51 2 1 2 217 851 0.0 6 832 19 218 672 0.0 6 661 10 0 0 1 219 643 0.0 6 626 16 0 1 220 418 0.0 6 402 16 221 551 0.0 6 542 7 0 0 2 222 700 0.0 6 681 18 0 0 1 223 551 0.0 6 543 7 0 0 1 224 338 0.0 6 329 8 0 0 1 225 279 0.0 6 269 7 2 1 226 642 0.0 6 618 22 0 1 1 227 1073 0.0 6 1035 37 0 0 1 228 799 0.0 6 772 25 1 0 1 229 1527 0.0 6 1478 46 1 0 2 230 3952 0.0 6 3854 92 4 0 2 231 762 0.0 6 731 31 232 1613 0.0 6 1578 33 0 1 1 233 245 0.0 6 237 8 234 1067 0.0 6 1030 35 0 0 2 235 446 0.0 6 430 15 0 1 236 509 0.0 6 497 11 1 237 544 0.0 6 529 15 238 287 0.0 6 278 9 239 48 0.0 6 47 0 0 0 0 1 240 142 0.0 6 138 4 241 249 0.0 6 244 5 242 150 0.0 6 147 3 243 118 0.0 6 114 4 244 162 0.0 6 155 7 245 465 0.0 6 462 3 246 328 0.0 6 318 8 0 0 2 247 203 0.0 6 198 5 248 156 0.0 6 154 2 249 285 0.0 6 275 10 250 137 0.0 6 133 4 251 441 0.0 6 433 8 252 207 0.0 6 198 9 253 232 0.0 6 216 16 254 64 0.0 6 61 3 255 48 0.0 6 47 1 256 45 0.0 6 45 257 2 0.0 6 2 258 1 0.0 6 0 1 260 2 0.0 6 2 264 2 0.0 6 2 266 1 0.0 6 1 267 1 0.0 6 1 279 5 0.0 6 4 0 0 0 0 1 280 37 0.0 6 36 1 281 25 0.0 6 21 4 282 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 49 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 73 1 0.0 6 1 75 1 0.0 6 1 76 1 0.0 6 1 77 1 0.0 6 1 78 3 0.0 6 3 79 1 0.0 6 1 81 1 0.0 6 1 82 1 0.0 6 1 84 1 0.0 6 1 86 1 0.0 6 1 87 2 0.0 6 2 88 2 0.0 6 2 89 1 0.0 6 1 90 2 0.0 6 2 93 1 0.0 6 1 94 1 0.0 6 1 96 1 0.0 6 1 98 1 0.0 6 1 102 1 0.0 6 1 111 2 0.0 6 2 113 1 0.0 6 1 116 1 0.0 6 1 123 1 0.0 6 1 125 2 0.0 6 1 0 0 1 126 1 0.0 6 1 139 1 0.0 6 1 140 1 0.0 6 1 143 1 0.0 6 1 148 1 0.0 6 1 149 1 0.0 6 1 151 1 0.0 6 1 153 1 0.0 6 1 163 1 0.0 6 1 167 1 0.0 6 1 168 1 0.0 6 1 173 1 0.0 6 1 175 1 0.0 6 1 178 3 0.0 6 3 184 1 0.0 6 1 211 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_20_11_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_20_238_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_20_11_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_20_238_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.24 s (47888 µs/read; 0.00 M reads/minute). === Summary === Total read pairs processed: 5 Read 1 with adapter: 5 (100.0%) Read 2 with adapter: 5 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 0 (0.0%) Pairs written (passing filters): 5 (100.0%) Total basepairs processed: 2,019 bp Read 1: 1,145 bp Read 2: 874 bp Total written (filtered): 314 bp (15.6%) Read 1: 154 bp Read 2: 160 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 5 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 111 1 0.0 6 0 1 183 1 0.0 6 1 214 1 0.0 6 1 241 1 0.0 6 1 242 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 0 times === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 5 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 110 1 0.0 6 1 111 1 0.0 6 1 133 1 0.0 6 1 146 1 0.0 6 1 214 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 0 times Running external command line application. This may print messages to stdout and/or stderr. The commands to be run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist. Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_01_0_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_01_227_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_01_0_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_01_227_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_02_1_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_02_228_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_02_1_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_02_228_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_03_2_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_03_229_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_03_2_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_03_229_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_04_3_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_04_230_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_04_3_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_04_230_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_05_4_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_05_231_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_05_4_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_05_231_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_06_5_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_06_232_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_06_5_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_06_232_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_15_6_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_15_233_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_15_6_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_15_233_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_16_7_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_16_234_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_16_7_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_16_234_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_17_8_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_17_235_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_17_8_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_17_235_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_18_9_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_18_236_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_18_9_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_18_236_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_19_10_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_19_237_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_19_10_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_19_237_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_20_11_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_01-Rev_20_238_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_20_11_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_01-Rev_20_238_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_01_12_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_01_239_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_01_12_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_01_239_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.93 s (28 µs/read; 2.12 M reads/minute). === Summary === Total read pairs processed: 68,219 Read 1 with adapter: 68,216 (100.0%) Read 2 with adapter: 68,183 (99.9%) == Read fate breakdown == Pairs that were too short: 4 (0.0%) Pairs discarded as untrimmed: 39 (0.1%) Pairs written (passing filters): 68,176 (99.9%) Total basepairs processed: 33,329,625 bp Read 1: 19,024,022 bp Read 2: 14,305,603 bp Total written (filtered): 4,239,454 bp (12.7%) Read 1: 2,189,573 bp Read 2: 2,049,881 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 68033 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1065.9 0 1 4 1 266.5 1 0 1 6 2 16.7 1 1 0 1 7 4 4.2 2 3 0 1 9 1 0.3 2 1 12 1 0.0 3 1 15 1 0.0 4 1 16 2 0.0 4 1 1 20 31 0.0 6 29 2 21 16 0.0 6 15 1 22 7 0.0 6 5 2 23 6 0.0 6 5 1 24 5 0.0 6 5 25 8 0.0 6 8 26 1 0.0 6 1 27 4 0.0 6 4 28 7 0.0 6 7 29 1 0.0 6 1 30 3 0.0 6 2 1 31 1 0.0 6 1 32 2 0.0 6 2 34 3 0.0 6 3 35 1 0.0 6 1 38 2 0.0 6 2 39 4 0.0 6 3 0 1 40 8 0.0 6 8 41 1 0.0 6 1 42 2 0.0 6 2 43 1 0.0 6 1 44 6 0.0 6 6 45 5 0.0 6 5 46 2 0.0 6 2 47 1 0.0 6 1 49 2 0.0 6 2 50 3 0.0 6 2 1 52 1 0.0 6 1 53 3 0.0 6 2 1 54 5 0.0 6 4 1 56 7 0.0 6 6 0 0 0 0 1 57 3 0.0 6 2 1 58 2 0.0 6 2 59 3 0.0 6 3 60 2 0.0 6 2 61 1 0.0 6 0 1 62 2 0.0 6 2 63 2 0.0 6 2 64 3 0.0 6 3 65 6 0.0 6 6 66 2 0.0 6 2 67 1 0.0 6 0 1 68 3 0.0 6 3 69 4 0.0 6 4 70 2 0.0 6 2 71 1 0.0 6 1 72 2 0.0 6 2 75 3 0.0 6 2 1 77 1 0.0 6 1 78 4 0.0 6 3 1 79 3 0.0 6 2 1 80 5 0.0 6 5 81 1 0.0 6 1 82 3 0.0 6 2 1 83 3 0.0 6 3 84 2 0.0 6 2 85 4 0.0 6 4 86 9 0.0 6 9 87 4 0.0 6 3 1 88 2 0.0 6 2 89 6 0.0 6 6 90 6 0.0 6 6 91 4 0.0 6 4 92 3 0.0 6 2 0 1 93 4 0.0 6 3 0 0 1 94 3 0.0 6 3 95 3 0.0 6 3 96 1 0.0 6 1 97 3 0.0 6 3 98 6 0.0 6 3 3 99 2 0.0 6 2 100 5 0.0 6 5 101 8 0.0 6 6 1 1 102 7 0.0 6 7 103 4 0.0 6 3 1 104 4 0.0 6 3 1 105 8 0.0 6 8 106 3 0.0 6 3 107 6 0.0 6 6 108 17 0.0 6 16 1 109 8 0.0 6 7 1 110 3 0.0 6 3 111 6 0.0 6 5 1 112 11 0.0 6 11 113 8 0.0 6 8 114 2 0.0 6 2 115 6 0.0 6 4 1 0 0 0 1 116 5 0.0 6 5 117 4 0.0 6 4 118 4 0.0 6 4 119 12 0.0 6 11 1 120 9 0.0 6 8 0 1 121 16 0.0 6 13 3 122 17 0.0 6 14 3 123 12 0.0 6 11 1 124 12 0.0 6 11 1 125 17 0.0 6 16 0 1 126 23 0.0 6 21 2 127 20 0.0 6 18 2 128 16 0.0 6 14 2 129 9 0.0 6 8 1 130 13 0.0 6 13 131 14 0.0 6 14 132 13 0.0 6 13 133 16 0.0 6 15 1 134 24 0.0 6 22 2 135 34 0.0 6 33 1 136 24 0.0 6 22 2 137 29 0.0 6 28 1 138 26 0.0 6 26 139 25 0.0 6 24 1 140 43 0.0 6 42 1 141 85 0.0 6 84 1 142 154 0.0 6 153 1 143 188 0.0 6 187 1 144 104 0.0 6 104 145 137 0.0 6 136 1 146 149 0.0 6 148 1 147 124 0.0 6 123 1 148 124 0.0 6 123 1 149 111 0.0 6 111 150 91 0.0 6 88 3 151 110 0.0 6 109 1 152 91 0.0 6 90 1 153 64 0.0 6 63 1 154 81 0.0 6 80 1 155 103 0.0 6 102 1 156 73 0.0 6 73 157 60 0.0 6 57 2 1 158 89 0.0 6 89 159 73 0.0 6 73 160 100 0.0 6 100 161 82 0.0 6 81 1 162 75 0.0 6 73 2 163 76 0.0 6 74 2 164 92 0.0 6 92 165 79 0.0 6 79 166 77 0.0 6 76 1 167 67 0.0 6 66 1 168 57 0.0 6 56 1 169 86 0.0 6 85 0 0 1 170 99 0.0 6 97 2 171 90 0.0 6 87 3 172 102 0.0 6 100 2 173 113 0.0 6 112 1 174 94 0.0 6 92 2 175 96 0.0 6 93 3 176 120 0.0 6 118 2 177 89 0.0 6 89 178 94 0.0 6 93 0 0 1 179 260 0.0 6 256 4 180 121 0.0 6 120 1 181 105 0.0 6 104 1 182 127 0.0 6 125 2 183 98 0.0 6 96 1 0 0 0 1 184 112 0.0 6 110 2 185 155 0.0 6 151 4 186 155 0.0 6 153 2 187 122 0.0 6 121 1 188 133 0.0 6 128 5 189 127 0.0 6 121 6 190 142 0.0 6 139 3 191 145 0.0 6 142 3 192 162 0.0 6 156 6 193 227 0.0 6 221 6 194 213 0.0 6 208 5 195 272 0.0 6 264 8 196 279 0.0 6 270 9 197 291 0.0 6 282 9 198 326 0.0 6 315 10 1 199 262 0.0 6 254 8 200 281 0.0 6 271 10 201 270 0.0 6 260 10 202 252 0.0 6 246 4 1 0 0 0 1 203 311 0.0 6 305 6 204 214 0.0 6 208 6 205 319 0.0 6 313 6 206 362 0.0 6 353 8 0 1 207 315 0.0 6 311 4 208 301 0.0 6 298 3 209 267 0.0 6 261 6 210 246 0.0 6 241 5 211 242 0.0 6 239 3 212 278 0.0 6 267 11 213 303 0.0 6 298 4 1 214 436 0.0 6 431 5 215 360 0.0 6 347 11 0 1 1 216 344 0.0 6 338 6 217 310 0.0 6 307 3 218 346 0.0 6 338 8 219 331 0.0 6 321 10 220 269 0.0 6 261 7 0 1 221 229 0.0 6 226 3 222 370 0.0 6 356 14 223 429 0.0 6 420 9 224 370 0.0 6 359 11 225 467 0.0 6 455 11 0 0 1 226 458 0.0 6 449 9 227 443 0.0 6 437 6 228 348 0.0 6 342 4 2 229 297 0.0 6 292 5 230 329 0.0 6 321 8 231 352 0.0 6 341 11 232 328 0.0 6 326 1 1 233 378 0.0 6 369 9 234 324 0.0 6 316 6 1 0 1 235 281 0.0 6 279 2 236 292 0.0 6 286 6 237 366 0.0 6 358 8 238 571 0.0 6 562 9 239 568 0.0 6 550 17 1 240 614 0.0 6 603 11 241 512 0.0 6 494 18 242 689 0.0 6 676 13 243 429 0.0 6 415 14 244 435 0.0 6 423 11 1 245 442 0.0 6 428 13 1 246 428 0.0 6 417 9 2 247 331 0.0 6 322 9 248 356 0.0 6 343 12 1 249 498 0.0 6 489 9 250 373 0.0 6 366 6 1 251 565 0.0 6 554 11 252 427 0.0 6 420 6 1 253 380 0.0 6 370 9 1 254 565 0.0 6 554 9 1 1 255 478 0.0 6 465 13 256 554 0.0 6 539 15 257 773 0.0 6 762 11 258 583 0.0 6 566 16 1 259 796 0.0 6 773 23 260 1177 0.0 6 1156 20 1 261 446 0.0 6 435 11 262 485 0.0 6 476 7 1 1 263 619 0.0 6 608 9 2 264 400 0.0 6 392 8 265 541 0.0 6 522 17 2 266 732 0.0 6 716 16 267 644 0.0 6 635 9 268 559 0.0 6 537 21 1 269 1492 0.0 6 1456 36 270 816 0.0 6 795 21 271 752 0.0 6 735 16 1 272 945 0.0 6 931 14 273 1169 0.0 6 1146 23 274 1980 0.0 6 1945 35 275 1156 0.0 6 1124 31 1 276 1123 0.0 6 1083 40 277 2154 0.0 6 2101 52 1 278 8507 0.0 6 8270 230 6 1 279 10107 0.0 6 9857 245 4 0 0 0 1 280 58 0.0 6 49 8 1 281 21 0.0 6 18 3 282 1 0.0 6 0 1 283 1 0.0 6 1 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 183 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 4 0.0 6 4 71 2 0.0 6 2 73 3 0.0 6 3 74 5 0.0 6 5 75 2 0.0 6 2 76 1 0.0 6 1 77 5 0.0 6 5 78 6 0.0 6 6 80 1 0.0 6 1 81 3 0.0 6 3 82 1 0.0 6 1 84 1 0.0 6 1 85 1 0.0 6 1 86 1 0.0 6 1 88 1 0.0 6 1 92 1 0.0 6 1 94 4 0.0 6 4 95 1 0.0 6 1 96 1 0.0 6 1 97 2 0.0 6 2 100 6 0.0 6 6 101 1 0.0 6 1 103 2 0.0 6 2 104 3 0.0 6 3 105 1 0.0 6 1 106 1 0.0 6 1 107 1 0.0 6 1 108 1 0.0 6 1 109 3 0.0 6 3 110 1 0.0 6 1 112 1 0.0 6 1 113 1 0.0 6 1 114 2 0.0 6 2 115 1 0.0 6 1 116 1 0.0 6 1 118 2 0.0 6 2 120 1 0.0 6 1 127 2 0.0 6 2 128 3 0.0 6 3 129 3 0.0 6 3 135 1 0.0 6 1 136 1 0.0 6 1 138 1 0.0 6 1 141 4 0.0 6 4 142 2 0.0 6 2 143 1 0.0 6 1 144 2 0.0 6 2 145 2 0.0 6 2 146 1 0.0 6 1 148 1 0.0 6 1 149 2 0.0 6 2 150 2 0.0 6 2 151 1 0.0 6 1 158 1 0.0 6 1 159 1 0.0 6 1 162 1 0.0 6 1 167 2 0.0 6 2 168 1 0.0 6 1 169 1 0.0 6 1 171 2 0.0 6 2 173 1 0.0 6 1 174 2 0.0 6 2 177 2 0.0 6 2 179 2 0.0 6 2 180 2 0.0 6 2 182 1 0.0 6 1 185 2 0.0 6 2 186 3 0.0 6 3 187 1 0.0 6 1 188 2 0.0 6 2 189 1 0.0 6 1 190 1 0.0 6 1 192 1 0.0 6 1 193 2 0.0 6 2 194 1 0.0 6 1 195 2 0.0 6 2 196 3 0.0 6 3 200 2 0.0 6 2 201 1 0.0 6 1 204 1 0.0 6 1 206 1 0.0 6 1 208 1 0.0 6 1 210 2 0.0 6 2 212 1 0.0 6 1 214 1 0.0 6 1 215 3 0.0 6 3 216 1 0.0 6 1 217 1 0.0 6 1 218 1 0.0 6 1 219 2 0.0 6 2 221 1 0.0 6 1 223 2 0.0 6 2 224 2 0.0 6 2 227 1 0.0 6 1 228 1 0.0 6 1 229 1 0.0 6 1 230 1 0.0 6 1 231 1 0.0 6 1 232 1 0.0 6 1 235 1 0.0 6 1 241 3 0.0 6 3 244 1 0.0 6 1 245 2 0.0 6 2 251 1 0.0 6 1 270 1 0.0 6 1 276 1 0.0 6 1 281 1 0.0 6 1 292 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 68044 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 4.2 2 1 11 3 0.0 3 3 12 6 0.0 3 6 13 15 0.0 3 13 2 14 6 0.0 4 6 15 2 0.0 4 1 1 16 9 0.0 4 8 0 1 17 8 0.0 5 6 2 18 6 0.0 5 5 1 19 9 0.0 5 8 1 20 50 0.0 6 48 2 21 59 0.0 6 59 22 32 0.0 6 32 23 40 0.0 6 37 3 24 32 0.0 6 30 2 25 29 0.0 6 27 2 26 9 0.0 6 9 27 9 0.0 6 8 1 28 11 0.0 6 10 1 29 19 0.0 6 19 30 9 0.0 6 8 1 31 12 0.0 6 11 1 32 16 0.0 6 13 2 0 0 1 33 9 0.0 6 9 34 16 0.0 6 16 35 8 0.0 6 8 36 7 0.0 6 7 37 13 0.0 6 12 1 38 13 0.0 6 13 39 6 0.0 6 6 40 12 0.0 6 11 0 0 0 1 41 6 0.0 6 5 0 1 42 8 0.0 6 6 2 43 8 0.0 6 7 1 44 12 0.0 6 12 45 9 0.0 6 8 1 46 10 0.0 6 7 3 47 16 0.0 6 15 1 48 12 0.0 6 10 2 49 19 0.0 6 18 1 50 20 0.0 6 19 1 51 9 0.0 6 9 52 20 0.0 6 19 1 53 11 0.0 6 8 3 54 25 0.0 6 23 1 0 0 1 55 28 0.0 6 25 3 56 25 0.0 6 19 6 57 16 0.0 6 15 1 58 13 0.0 6 12 1 59 18 0.0 6 15 2 1 60 21 0.0 6 20 1 61 11 0.0 6 10 1 62 16 0.0 6 14 2 63 15 0.0 6 12 2 0 1 64 16 0.0 6 15 1 65 13 0.0 6 10 3 66 23 0.0 6 23 67 28 0.0 6 22 5 1 68 29 0.0 6 26 2 1 69 135 0.0 6 134 1 70 29 0.0 6 25 4 71 78 0.0 6 76 2 72 41 0.0 6 37 4 73 21 0.0 6 19 2 74 15 0.0 6 15 75 14 0.0 6 14 76 27 0.0 6 25 2 77 27 0.0 6 26 1 78 20 0.0 6 15 5 79 24 0.0 6 23 1 80 26 0.0 6 25 0 0 0 1 81 21 0.0 6 18 3 82 34 0.0 6 31 2 0 1 83 51 0.0 6 46 4 0 0 0 1 84 51 0.0 6 45 6 85 34 0.0 6 29 3 1 0 1 86 29 0.0 6 26 2 0 0 1 87 51 0.0 6 48 3 88 13 0.0 6 13 89 39 0.0 6 34 3 2 90 56 0.0 6 53 3 91 27 0.0 6 26 1 92 38 0.0 6 34 4 93 68 0.0 6 62 3 1 1 1 94 86 0.0 6 81 4 1 95 46 0.0 6 42 4 96 57 0.0 6 55 2 97 50 0.0 6 48 1 1 98 57 0.0 6 55 2 99 60 0.0 6 57 3 100 56 0.0 6 54 1 1 101 45 0.0 6 41 3 0 0 0 0 1 102 88 0.0 6 83 5 103 93 0.0 6 90 3 104 73 0.0 6 71 2 105 99 0.0 6 97 2 106 73 0.0 6 70 2 1 107 68 0.0 6 64 4 108 73 0.0 6 70 3 109 72 0.0 6 69 3 110 64 0.0 6 62 1 1 111 74 0.0 6 70 3 0 0 0 1 112 86 0.0 6 77 8 0 0 0 1 113 65 0.0 6 61 3 1 114 104 0.0 6 99 4 0 0 1 115 86 0.0 6 83 3 116 69 0.0 6 65 4 117 56 0.0 6 52 4 118 83 0.0 6 79 3 0 0 1 119 88 0.0 6 84 4 120 135 0.0 6 133 1 1 121 88 0.0 6 83 4 1 122 107 0.0 6 99 6 2 123 111 0.0 6 107 4 124 124 0.0 6 117 7 125 102 0.0 6 97 4 0 0 1 126 92 0.0 6 90 1 1 127 135 0.0 6 129 6 128 121 0.0 6 119 2 129 79 0.0 6 76 2 1 130 98 0.0 6 94 2 1 0 0 1 131 199 0.0 6 196 2 0 0 1 132 235 0.0 6 225 7 0 0 2 0 1 133 212 0.0 6 207 4 0 0 1 134 248 0.0 6 245 2 0 1 135 382 0.0 6 366 15 0 0 1 136 250 0.0 6 236 13 1 137 304 0.0 6 297 5 2 138 206 0.0 6 205 1 139 255 0.0 6 249 3 1 1 1 140 314 0.0 6 305 9 141 375 0.0 6 371 4 142 494 0.0 6 479 13 1 0 0 0 1 143 381 0.0 6 372 8 0 0 1 144 567 0.0 6 554 12 1 145 364 0.0 6 352 8 2 1 1 146 334 0.0 6 329 3 0 1 0 0 1 147 259 0.0 6 251 8 148 378 0.0 6 369 7 2 149 477 0.0 6 464 10 1 0 2 150 623 0.0 6 610 11 1 0 1 151 389 0.0 6 378 11 152 878 0.0 6 859 19 153 488 0.0 6 477 11 154 621 0.0 6 613 8 155 560 0.0 6 547 11 2 156 433 0.0 6 422 10 1 157 628 0.0 6 613 15 158 613 0.0 6 587 24 2 159 379 0.0 6 366 10 1 0 1 1 160 598 0.0 6 583 15 161 382 0.0 6 370 11 0 0 0 1 162 383 0.0 6 375 7 0 0 1 163 520 0.0 6 507 11 1 0 1 164 376 0.0 6 365 10 1 165 493 0.0 6 483 9 0 0 1 166 564 0.0 6 553 11 167 297 0.0 6 292 4 0 1 168 773 0.0 6 756 10 1 1 4 0 1 169 288 0.0 6 280 8 170 415 0.0 6 410 5 171 620 0.0 6 605 15 172 274 0.0 6 266 7 0 0 0 0 1 173 418 0.0 6 402 15 1 174 610 0.0 6 582 27 1 175 1016 0.0 6 984 29 3 176 660 0.0 6 637 21 2 177 684 0.0 6 670 14 178 751 0.0 6 730 20 1 179 678 0.0 6 660 17 0 0 0 1 180 619 0.0 6 600 18 0 0 1 181 685 0.0 6 662 19 3 0 1 182 283 0.0 6 272 11 183 628 0.0 6 604 24 184 201 0.0 6 196 2 1 0 2 185 371 0.0 6 363 7 0 1 186 1448 0.0 6 1416 27 4 1 187 1290 0.0 6 1249 39 1 1 188 1422 0.0 6 1371 47 2 0 2 189 1321 0.0 6 1282 39 190 1807 0.0 6 1774 30 2 1 191 1308 0.0 6 1270 36 1 0 1 192 790 0.0 6 764 25 1 193 679 0.0 6 659 19 0 1 194 1116 0.0 6 1075 39 2 195 1827 0.0 6 1759 63 3 0 2 196 2075 0.0 6 2022 52 1 197 401 0.0 6 377 23 0 0 0 1 198 1910 0.0 6 1851 55 3 1 199 2899 0.0 6 2841 56 1 0 1 200 1066 0.0 6 1034 32 201 1106 0.0 6 1087 16 1 0 2 202 861 0.0 6 832 27 1 0 1 203 705 0.0 6 681 24 204 856 0.0 6 832 23 0 0 1 205 212 0.0 6 206 6 206 297 0.0 6 288 9 207 143 0.0 6 140 3 208 380 0.0 6 367 11 1 1 209 337 0.0 6 329 8 210 286 0.0 6 279 5 1 1 211 384 0.0 6 364 18 1 1 212 321 0.0 6 317 4 213 120 0.0 6 117 3 214 330 0.0 6 322 7 0 0 1 215 411 0.0 6 405 5 1 216 1053 0.0 6 1014 38 0 0 1 217 698 0.0 6 682 16 218 618 0.0 6 599 17 0 0 1 1 219 540 0.0 6 520 20 220 401 0.0 6 385 15 1 221 701 0.0 6 676 25 222 274 0.0 6 265 8 1 223 871 0.0 6 846 24 1 224 349 0.0 6 341 7 0 0 1 225 500 0.0 6 485 15 226 349 0.0 6 341 8 227 241 0.0 6 238 3 228 128 0.0 6 127 1 229 236 0.0 6 231 3 0 0 2 230 173 0.0 6 170 3 231 200 0.0 6 192 8 232 247 0.0 6 239 6 0 1 0 0 1 233 402 0.0 6 388 13 1 234 173 0.0 6 169 3 1 235 77 0.0 6 75 2 236 51 0.0 6 47 4 237 59 0.0 6 56 3 238 15 0.0 6 14 1 239 12 0.0 6 12 240 114 0.0 6 109 5 241 89 0.0 6 86 3 242 149 0.0 6 148 1 243 204 0.0 6 198 5 1 244 121 0.0 6 117 4 245 64 0.0 6 60 2 0 0 2 246 58 0.0 6 55 3 247 23 0.0 6 23 248 40 0.0 6 39 1 249 41 0.0 6 39 2 250 27 0.0 6 26 1 251 18 0.0 6 17 1 252 5 0.0 6 5 253 28 0.0 6 27 1 254 8 0.0 6 8 255 5 0.0 6 5 257 1 0.0 6 1 258 1 0.0 6 0 1 277 1 0.0 6 1 278 3 0.0 6 3 279 5 0.0 6 5 280 25 0.0 6 25 281 139 0.0 6 136 3 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 139 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 52 1 0.0 6 1 59 1 0.0 6 1 63 1 0.0 6 1 70 1 0.0 6 1 71 1 0.0 6 1 72 1 0.0 6 1 73 1 0.0 6 1 74 5 0.0 6 5 75 4 0.0 6 4 76 1 0.0 6 1 77 6 0.0 6 6 78 1 0.0 6 1 79 1 0.0 6 1 80 2 0.0 6 2 81 1 0.0 6 1 82 1 0.0 6 1 83 1 0.0 6 1 84 1 0.0 6 1 86 1 0.0 6 1 87 4 0.0 6 4 89 2 0.0 6 2 90 1 0.0 6 1 91 3 0.0 6 3 92 1 0.0 6 1 93 1 0.0 6 1 94 4 0.0 6 4 95 2 0.0 6 2 96 2 0.0 6 2 98 2 0.0 6 2 99 2 0.0 6 2 100 2 0.0 6 2 102 1 0.0 6 1 105 4 0.0 6 3 1 106 1 0.0 6 1 107 3 0.0 6 3 108 5 0.0 6 5 109 2 0.0 6 2 112 4 0.0 6 3 0 0 0 1 114 1 0.0 6 1 119 1 0.0 6 1 120 1 0.0 6 1 122 1 0.0 6 1 123 3 0.0 6 3 127 2 0.0 6 2 132 2 0.0 6 2 137 1 0.0 6 1 138 2 0.0 6 2 139 1 0.0 6 1 140 1 0.0 6 1 146 1 0.0 6 1 147 3 0.0 6 3 149 1 0.0 6 1 150 3 0.0 6 3 155 1 0.0 6 1 156 2 0.0 6 2 157 3 0.0 6 3 158 1 0.0 6 1 159 1 0.0 6 1 160 2 0.0 6 2 162 3 0.0 6 3 165 2 0.0 6 2 171 1 0.0 6 1 173 1 0.0 6 1 174 1 0.0 6 0 1 175 1 0.0 6 1 177 1 0.0 6 1 178 1 0.0 6 1 179 1 0.0 6 1 180 1 0.0 6 1 182 2 0.0 6 2 184 1 0.0 6 1 187 1 0.0 6 1 190 1 0.0 6 1 191 1 0.0 6 1 192 2 0.0 6 2 197 2 0.0 6 2 198 1 0.0 6 1 199 1 0.0 6 1 210 1 0.0 6 1 216 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_02_13_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_02_240_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_02_13_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_02_240_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.73 s (28 µs/read; 2.17 M reads/minute). === Summary === Total read pairs processed: 62,806 Read 1 with adapter: 62,797 (100.0%) Read 2 with adapter: 62,806 (100.0%) == Read fate breakdown == Pairs that were too short: 30 (0.0%) Pairs discarded as untrimmed: 9 (0.0%) Pairs written (passing filters): 62,767 (99.9%) Total basepairs processed: 26,930,552 bp Read 1: 15,238,905 bp Read 2: 11,691,647 bp Total written (filtered): 4,040,546 bp (15.0%) Read 1: 2,070,290 bp Read 2: 1,970,256 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 61323 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 981.3 0 0 2 4 3 245.3 1 1 2 6 2 15.3 1 0 0 2 7 4 3.8 2 3 0 1 8 1 1.0 2 0 1 9 1 0.2 2 0 1 13 1 0.0 3 1 15 3 0.0 4 2 1 16 2 0.0 4 1 1 17 5 0.0 5 5 18 1 0.0 5 1 19 3 0.0 5 2 1 20 65 0.0 6 63 1 1 21 33 0.0 6 32 1 22 25 0.0 6 24 1 23 13 0.0 6 13 24 20 0.0 6 16 3 0 1 25 21 0.0 6 19 2 26 14 0.0 6 12 1 1 27 13 0.0 6 10 2 1 28 6 0.0 6 6 29 7 0.0 6 6 1 30 7 0.0 6 2 2 1 0 0 1 1 31 6 0.0 6 5 0 1 32 5 0.0 6 4 0 0 1 33 4 0.0 6 3 1 34 3 0.0 6 3 35 2 0.0 6 2 36 6 0.0 6 6 37 5 0.0 6 5 38 8 0.0 6 7 1 39 6 0.0 6 6 40 6 0.0 6 6 41 6 0.0 6 6 42 6 0.0 6 6 43 6 0.0 6 6 44 8 0.0 6 8 45 4 0.0 6 4 46 6 0.0 6 6 47 4 0.0 6 4 48 7 0.0 6 7 49 4 0.0 6 4 50 9 0.0 6 9 51 6 0.0 6 5 1 52 4 0.0 6 4 53 4 0.0 6 4 54 3 0.0 6 2 1 55 5 0.0 6 5 56 2 0.0 6 2 57 8 0.0 6 8 58 3 0.0 6 3 59 6 0.0 6 4 2 60 3 0.0 6 2 1 61 7 0.0 6 7 62 6 0.0 6 5 1 63 7 0.0 6 7 64 4 0.0 6 4 65 7 0.0 6 7 66 6 0.0 6 4 1 0 1 67 13 0.0 6 12 1 68 7 0.0 6 7 69 4 0.0 6 3 1 70 7 0.0 6 7 71 10 0.0 6 10 72 15 0.0 6 13 2 73 7 0.0 6 5 2 74 8 0.0 6 7 1 75 12 0.0 6 12 76 6 0.0 6 6 77 6 0.0 6 6 78 3 0.0 6 3 79 6 0.0 6 5 1 80 6 0.0 6 5 1 81 8 0.0 6 8 82 9 0.0 6 9 83 15 0.0 6 15 84 10 0.0 6 10 85 8 0.0 6 8 86 7 0.0 6 7 87 7 0.0 6 6 1 88 8 0.0 6 8 89 10 0.0 6 10 90 5 0.0 6 5 91 6 0.0 6 6 92 7 0.0 6 7 93 5 0.0 6 5 94 17 0.0 6 17 95 12 0.0 6 11 1 96 6 0.0 6 6 97 12 0.0 6 12 98 11 0.0 6 11 99 15 0.0 6 15 100 11 0.0 6 9 2 101 9 0.0 6 8 1 102 12 0.0 6 11 1 103 21 0.0 6 20 1 104 18 0.0 6 18 105 10 0.0 6 9 1 106 12 0.0 6 11 1 107 8 0.0 6 8 108 19 0.0 6 19 109 14 0.0 6 14 110 21 0.0 6 21 111 13 0.0 6 13 112 21 0.0 6 21 113 19 0.0 6 19 114 15 0.0 6 14 1 115 32 0.0 6 30 2 116 14 0.0 6 14 117 16 0.0 6 15 1 118 26 0.0 6 25 1 119 27 0.0 6 26 1 120 17 0.0 6 17 121 21 0.0 6 20 1 122 29 0.0 6 28 1 123 22 0.0 6 20 2 124 29 0.0 6 28 1 125 24 0.0 6 21 3 126 27 0.0 6 26 0 0 0 0 1 127 25 0.0 6 25 128 29 0.0 6 29 129 37 0.0 6 36 1 130 38 0.0 6 36 2 131 34 0.0 6 32 2 132 47 0.0 6 47 133 27 0.0 6 27 134 41 0.0 6 41 135 41 0.0 6 41 136 44 0.0 6 43 1 137 34 0.0 6 34 138 51 0.0 6 51 139 45 0.0 6 45 140 80 0.0 6 76 3 0 1 141 57 0.0 6 57 142 149 0.0 6 147 2 143 265 0.0 6 263 2 144 272 0.0 6 272 145 276 0.0 6 274 2 146 257 0.0 6 257 147 337 0.0 6 337 148 312 0.0 6 310 2 149 346 0.0 6 345 1 150 324 0.0 6 322 1 0 1 151 246 0.0 6 242 3 1 152 331 0.0 6 330 0 0 0 0 0 1 153 234 0.0 6 233 1 154 192 0.0 6 192 155 227 0.0 6 223 3 0 0 1 156 229 0.0 6 227 2 157 201 0.0 6 201 158 196 0.0 6 191 4 0 0 0 1 159 247 0.0 6 246 1 160 220 0.0 6 219 1 161 230 0.0 6 229 1 162 230 0.0 6 230 163 271 0.0 6 271 164 236 0.0 6 232 4 165 280 0.0 6 277 2 0 0 0 1 166 212 0.0 6 210 2 167 213 0.0 6 211 2 168 252 0.0 6 249 3 169 220 0.0 6 220 170 236 0.0 6 232 0 0 0 1 2 1 171 264 0.0 6 261 3 172 263 0.0 6 259 4 173 234 0.0 6 234 174 282 0.0 6 281 1 175 277 0.0 6 274 3 176 237 0.0 6 233 4 177 242 0.0 6 240 1 0 0 1 178 246 0.0 6 241 4 0 0 0 1 179 294 0.0 6 292 2 180 538 0.0 6 537 1 181 294 0.0 6 292 2 182 280 0.0 6 278 1 0 0 0 1 183 315 0.0 6 312 3 184 317 0.0 6 315 1 0 1 185 317 0.0 6 313 3 1 186 358 0.0 6 356 2 187 342 0.0 6 338 4 188 328 0.0 6 327 1 189 354 0.0 6 350 2 0 1 0 1 190 340 0.0 6 339 1 191 382 0.0 6 377 4 1 192 488 0.0 6 484 3 0 0 0 1 193 454 0.0 6 450 3 1 194 532 0.0 6 525 5 0 0 0 1 1 195 600 0.0 6 597 3 196 666 0.0 6 664 2 197 745 0.0 6 740 4 0 0 0 1 198 910 0.0 6 899 11 199 649 0.0 6 643 6 200 574 0.0 6 563 9 0 1 0 1 201 645 0.0 6 641 3 0 0 0 1 202 525 0.0 6 521 2 1 0 0 0 1 203 602 0.0 6 597 5 204 717 0.0 6 714 3 205 673 0.0 6 664 8 1 206 832 0.0 6 822 9 1 207 694 0.0 6 683 8 2 0 0 0 1 208 716 0.0 6 712 4 209 601 0.0 6 599 2 210 631 0.0 6 628 3 211 796 0.0 6 789 6 0 1 212 758 0.0 6 750 6 1 0 0 1 213 815 0.0 6 808 6 0 0 0 1 214 941 0.0 6 934 7 215 1010 0.0 6 998 9 0 1 1 0 1 216 821 0.0 6 811 10 217 819 0.0 6 814 4 0 0 0 0 1 218 837 0.0 6 830 5 0 1 0 0 1 219 794 0.0 6 791 1 1 0 0 1 220 633 0.0 6 627 6 221 691 0.0 6 689 2 222 625 0.0 6 622 3 223 659 0.0 6 653 6 224 670 0.0 6 668 2 225 869 0.0 6 858 11 226 765 0.0 6 759 6 227 643 0.0 6 643 228 669 0.0 6 664 4 0 0 0 1 229 618 0.0 6 613 5 230 662 0.0 6 658 4 231 644 0.0 6 638 6 232 669 0.0 6 665 3 1 233 653 0.0 6 652 1 234 575 0.0 6 572 2 1 235 522 0.0 6 521 0 0 0 0 0 1 236 499 0.0 6 495 1 0 0 1 0 2 237 598 0.0 6 593 3 1 0 0 0 1 238 586 0.0 6 580 6 239 501 0.0 6 498 2 0 0 0 1 240 541 0.0 6 541 241 437 0.0 6 436 1 242 460 0.0 6 458 2 243 483 0.0 6 483 244 396 0.0 6 394 2 245 355 0.0 6 352 3 246 319 0.0 6 316 3 247 347 0.0 6 346 1 248 310 0.0 6 310 249 303 0.0 6 301 2 250 321 0.0 6 317 3 0 0 1 251 338 0.0 6 336 2 252 279 0.0 6 274 5 253 296 0.0 6 295 0 1 254 268 0.0 6 267 1 255 261 0.0 6 259 2 256 236 0.0 6 234 2 257 250 0.0 6 248 2 258 218 0.0 6 215 3 259 257 0.0 6 252 5 260 225 0.0 6 224 1 261 187 0.0 6 187 262 163 0.0 6 163 263 190 0.0 6 190 264 180 0.0 6 179 1 265 168 0.0 6 167 1 266 178 0.0 6 177 1 267 166 0.0 6 165 1 268 162 0.0 6 161 1 269 172 0.0 6 172 270 144 0.0 6 143 1 271 116 0.0 6 115 1 272 131 0.0 6 130 1 273 169 0.0 6 166 2 0 0 0 0 1 274 221 0.0 6 220 1 275 225 0.0 6 223 2 276 279 0.0 6 277 2 277 360 0.0 6 358 2 278 1322 0.0 6 1307 14 0 0 1 279 1664 0.0 6 1647 15 0 1 1 280 11 0.0 6 11 281 1 0.0 6 1 282 2 0.0 6 2 283 1 0.0 6 1 284 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1474 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 4 0.0 6 0 0 2 2 62 8 0.0 6 2 6 63 72 0.0 6 68 4 64 1 0.0 6 1 65 1 0.0 6 1 66 1 0.0 6 1 70 2 0.0 6 2 71 5 0.0 6 5 72 8 0.0 6 8 73 6 0.0 6 6 74 13 0.0 6 13 75 11 0.0 6 11 76 9 0.0 6 9 77 9 0.0 6 9 78 12 0.0 6 12 79 9 0.0 6 9 80 13 0.0 6 13 81 13 0.0 6 13 82 6 0.0 6 6 83 7 0.0 6 7 84 1 0.0 6 1 85 3 0.0 6 3 86 7 0.0 6 7 87 7 0.0 6 7 88 4 0.0 6 4 89 6 0.0 6 6 90 7 0.0 6 7 91 6 0.0 6 6 92 6 0.0 6 6 93 8 0.0 6 8 94 1 0.0 6 1 95 8 0.0 6 8 96 4 0.0 6 4 97 6 0.0 6 6 98 6 0.0 6 6 99 1 0.0 6 1 100 3 0.0 6 3 101 3 0.0 6 3 102 9 0.0 6 9 103 9 0.0 6 9 104 3 0.0 6 3 105 6 0.0 6 6 106 3 0.0 6 3 107 6 0.0 6 6 108 8 0.0 6 8 109 7 0.0 6 7 110 12 0.0 6 12 111 3 0.0 6 3 112 4 0.0 6 4 113 3 0.0 6 3 114 1 0.0 6 1 115 2 0.0 6 2 116 6 0.0 6 6 117 5 0.0 6 5 118 1 0.0 6 1 119 4 0.0 6 4 120 7 0.0 6 7 121 5 0.0 6 5 122 5 0.0 6 5 123 1 0.0 6 1 124 7 0.0 6 7 125 5 0.0 6 5 126 2 0.0 6 2 127 7 0.0 6 7 128 9 0.0 6 9 129 3 0.0 6 3 130 5 0.0 6 5 131 5 0.0 6 5 132 6 0.0 6 6 133 9 0.0 6 9 134 8 0.0 6 8 135 6 0.0 6 6 136 6 0.0 6 6 137 8 0.0 6 8 138 3 0.0 6 3 139 9 0.0 6 9 140 8 0.0 6 8 141 7 0.0 6 7 142 9 0.0 6 9 143 5 0.0 6 5 144 12 0.0 6 12 145 4 0.0 6 4 146 4 0.0 6 4 147 9 0.0 6 9 148 3 0.0 6 3 149 8 0.0 6 8 150 10 0.0 6 10 151 10 0.0 6 10 152 6 0.0 6 6 153 1 0.0 6 1 154 3 0.0 6 3 155 5 0.0 6 5 156 6 0.0 6 6 157 7 0.0 6 7 158 8 0.0 6 8 159 9 0.0 6 9 160 4 0.0 6 4 161 6 0.0 6 6 162 10 0.0 6 10 163 12 0.0 6 12 164 13 0.0 6 13 165 10 0.0 6 10 166 10 0.0 6 10 167 7 0.0 6 7 168 5 0.0 6 5 169 14 0.0 6 14 170 7 0.0 6 7 171 12 0.0 6 12 172 6 0.0 6 6 173 10 0.0 6 9 1 174 13 0.0 6 13 175 6 0.0 6 6 176 8 0.0 6 8 177 6 0.0 6 6 178 8 0.0 6 8 179 14 0.0 6 14 180 3 0.0 6 3 181 8 0.0 6 8 182 13 0.0 6 13 183 9 0.0 6 9 184 9 0.0 6 9 185 13 0.0 6 13 186 9 0.0 6 9 187 13 0.0 6 13 188 17 0.0 6 17 189 14 0.0 6 14 190 8 0.0 6 8 191 7 0.0 6 7 192 13 0.0 6 13 193 14 0.0 6 14 194 9 0.0 6 9 195 10 0.0 6 10 196 7 0.0 6 7 197 12 0.0 6 12 198 10 0.0 6 10 199 9 0.0 6 9 200 12 0.0 6 12 201 10 0.0 6 10 202 11 0.0 6 11 203 9 0.0 6 9 204 12 0.0 6 12 205 9 0.0 6 9 206 13 0.0 6 13 207 9 0.0 6 9 208 10 0.0 6 10 209 5 0.0 6 5 210 7 0.0 6 7 211 4 0.0 6 4 212 11 0.0 6 11 213 7 0.0 6 7 214 10 0.0 6 10 215 11 0.0 6 11 216 5 0.0 6 5 217 9 0.0 6 9 218 7 0.0 6 7 219 6 0.0 6 6 220 10 0.0 6 10 221 10 0.0 6 10 222 10 0.0 6 10 223 6 0.0 6 6 224 9 0.0 6 9 225 7 0.0 6 7 226 6 0.0 6 6 227 6 0.0 6 6 228 11 0.0 6 11 229 3 0.0 6 3 230 10 0.0 6 10 231 5 0.0 6 5 232 5 0.0 6 5 233 3 0.0 6 3 234 10 0.0 6 10 235 4 0.0 6 4 236 11 0.0 6 11 237 6 0.0 6 6 238 6 0.0 6 6 239 10 0.0 6 10 240 3 0.0 6 3 241 8 0.0 6 8 242 5 0.0 6 5 243 2 0.0 6 2 244 6 0.0 6 6 245 6 0.0 6 6 246 2 0.0 6 2 247 3 0.0 6 3 248 10 0.0 6 10 249 5 0.0 6 5 250 5 0.0 6 5 251 5 0.0 6 5 252 3 0.0 6 3 253 1 0.0 6 1 254 1 0.0 6 1 255 3 0.0 6 3 256 2 0.0 6 2 257 2 0.0 6 2 259 2 0.0 6 2 261 5 0.0 6 5 262 2 0.0 6 2 263 2 0.0 6 2 265 2 0.0 6 2 266 4 0.0 6 4 267 4 0.0 6 4 268 2 0.0 6 2 269 1 0.0 6 1 270 3 0.0 6 3 271 4 0.0 6 4 272 3 0.0 6 3 273 1 0.0 6 1 274 1 0.0 6 1 275 1 0.0 6 1 276 1 0.0 6 1 277 2 0.0 6 2 280 1 0.0 6 1 283 1 0.0 6 1 288 1 0.0 6 1 290 1 0.0 6 1 292 2 0.0 6 2 293 1 0.0 6 1 297 1 0.0 6 1 300 1 0.0 6 1 306 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 61738 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 245.3 1 1 10 3 0.1 3 2 1 11 5 0.0 3 4 1 12 11 0.0 3 11 13 10 0.0 3 8 2 14 15 0.0 4 13 1 1 15 6 0.0 4 6 16 6 0.0 4 5 1 17 45 0.0 5 41 4 18 31 0.0 5 28 3 19 18 0.0 5 14 4 20 66 0.0 6 62 4 21 30 0.0 6 28 2 22 35 0.0 6 34 1 23 20 0.0 6 17 3 24 13 0.0 6 13 25 16 0.0 6 14 2 26 18 0.0 6 18 27 11 0.0 6 10 0 0 0 0 1 28 14 0.0 6 13 1 29 15 0.0 6 14 1 30 12 0.0 6 11 1 31 16 0.0 6 15 1 32 11 0.0 6 9 2 33 14 0.0 6 12 2 34 9 0.0 6 7 1 0 0 0 1 35 15 0.0 6 12 2 0 0 0 1 36 12 0.0 6 11 1 37 14 0.0 6 12 2 38 10 0.0 6 9 1 39 11 0.0 6 11 40 7 0.0 6 6 0 1 41 11 0.0 6 11 42 13 0.0 6 12 1 43 10 0.0 6 9 1 44 15 0.0 6 14 1 45 18 0.0 6 17 1 46 17 0.0 6 17 47 20 0.0 6 17 2 0 0 1 48 19 0.0 6 19 49 11 0.0 6 10 0 1 50 21 0.0 6 19 2 51 20 0.0 6 18 2 52 40 0.0 6 36 3 1 53 29 0.0 6 25 2 1 1 54 15 0.0 6 13 2 55 29 0.0 6 27 2 56 31 0.0 6 28 3 57 31 0.0 6 31 58 23 0.0 6 19 3 0 0 1 59 30 0.0 6 27 3 60 20 0.0 6 19 1 61 25 0.0 6 22 2 0 0 0 1 62 25 0.0 6 23 2 63 37 0.0 6 33 3 0 0 1 64 46 0.0 6 45 0 1 65 32 0.0 6 30 2 66 35 0.0 6 31 4 67 53 0.0 6 52 1 68 139 0.0 6 133 4 1 0 0 1 69 65 0.0 6 62 3 70 54 0.0 6 51 3 71 40 0.0 6 38 2 72 57 0.0 6 48 7 2 73 38 0.0 6 37 1 74 40 0.0 6 36 4 75 47 0.0 6 44 1 2 76 45 0.0 6 44 0 0 0 1 77 39 0.0 6 37 1 0 0 0 0 1 78 51 0.0 6 48 3 79 48 0.0 6 46 2 80 48 0.0 6 46 1 0 1 81 67 0.0 6 63 1 1 1 1 82 71 0.0 6 68 3 83 89 0.0 6 78 9 0 0 1 0 1 84 76 0.0 6 75 1 85 54 0.0 6 49 4 0 0 1 86 51 0.0 6 48 3 87 43 0.0 6 43 88 40 0.0 6 36 3 1 89 62 0.0 6 56 5 0 0 0 0 1 90 59 0.0 6 58 0 0 0 0 0 1 91 84 0.0 6 79 3 2 92 72 0.0 6 68 3 1 93 81 0.0 6 80 1 94 88 0.0 6 86 2 95 78 0.0 6 77 1 96 92 0.0 6 87 3 1 0 1 97 94 0.0 6 89 4 0 0 1 98 78 0.0 6 75 3 99 82 0.0 6 78 3 0 0 0 0 1 100 104 0.0 6 99 3 0 0 1 1 101 91 0.0 6 88 3 102 101 0.0 6 95 6 103 97 0.0 6 90 4 2 0 1 104 112 0.0 6 106 5 1 105 194 0.0 6 189 4 0 1 106 131 0.0 6 124 6 0 1 107 116 0.0 6 111 4 0 0 1 108 108 0.0 6 104 4 109 128 0.0 6 125 1 0 0 1 0 1 110 138 0.0 6 132 4 1 0 0 1 111 140 0.0 6 136 2 0 1 0 0 1 112 174 0.0 6 172 2 113 188 0.0 6 180 6 1 0 1 114 170 0.0 6 164 3 3 115 175 0.0 6 171 4 116 167 0.0 6 160 5 2 117 163 0.0 6 159 3 0 0 0 1 118 175 0.0 6 172 2 0 0 0 0 1 119 210 0.0 6 206 3 0 0 1 120 183 0.0 6 177 5 0 1 121 197 0.0 6 189 6 1 0 0 1 122 192 0.0 6 183 7 1 0 0 1 123 209 0.0 6 199 7 1 0 1 0 1 124 240 0.0 6 234 4 1 1 125 280 0.0 6 275 3 1 1 126 281 0.0 6 273 7 0 0 0 0 1 127 352 0.0 6 343 6 1 0 1 1 128 332 0.0 6 320 8 0 1 0 2 1 129 333 0.0 6 323 7 0 0 2 1 130 417 0.0 6 400 16 0 0 0 0 1 131 414 0.0 6 396 15 1 1 1 132 436 0.0 6 427 7 0 0 1 1 133 445 0.0 6 438 6 0 0 0 1 134 496 0.0 6 481 11 0 0 0 2 2 135 493 0.0 6 479 13 1 136 509 0.0 6 495 10 0 2 1 1 137 466 0.0 6 455 8 1 1 1 138 448 0.0 6 438 9 0 0 0 0 1 139 487 0.0 6 476 10 0 0 0 0 1 140 633 0.0 6 622 11 141 687 0.0 6 671 14 0 2 142 802 0.0 6 792 8 1 0 1 143 817 0.0 6 804 9 1 0 2 1 144 813 0.0 6 799 11 2 0 1 145 894 0.0 6 885 7 0 0 0 0 2 146 970 0.0 6 948 13 3 1 4 1 147 966 0.0 6 948 17 0 1 148 948 0.0 6 933 13 1 0 1 149 1065 0.0 6 1052 12 0 0 0 0 1 150 1244 0.0 6 1219 24 0 1 151 1505 0.0 6 1469 31 0 1 1 1 2 152 1172 0.0 6 1153 19 153 1313 0.0 6 1285 24 1 0 1 2 154 1264 0.0 6 1237 22 2 1 0 1 1 155 1056 0.0 6 1035 15 1 2 1 1 1 156 1132 0.0 6 1119 11 1 0 0 1 157 969 0.0 6 950 19 158 1092 0.0 6 1076 16 159 1076 0.0 6 1054 18 2 0 0 0 2 160 975 0.0 6 958 13 1 0 1 0 2 161 945 0.0 6 931 11 2 0 0 0 1 162 877 0.0 6 864 11 1 0 0 0 1 163 813 0.0 6 800 11 0 1 0 0 1 164 796 0.0 6 784 10 0 0 1 1 165 757 0.0 6 745 11 0 1 166 778 0.0 6 767 10 1 167 920 0.0 6 907 10 0 0 1 1 1 168 779 0.0 6 773 6 169 938 0.0 6 926 12 170 724 0.0 6 713 7 1 0 0 2 1 171 823 0.0 6 816 4 0 1 1 0 1 172 855 0.0 6 844 9 0 2 173 872 0.0 6 861 10 0 0 1 174 798 0.0 6 787 8 2 0 0 1 175 762 0.0 6 750 12 176 760 0.0 6 753 4 2 1 177 807 0.0 6 799 7 0 0 0 0 1 178 651 0.0 6 645 4 1 0 1 179 600 0.0 6 589 9 1 1 180 525 0.0 6 516 9 181 494 0.0 6 487 5 0 1 0 1 182 463 0.0 6 461 1 0 1 183 454 0.0 6 449 4 1 184 664 0.0 6 655 6 2 0 0 0 1 185 788 0.0 6 783 4 0 0 1 186 687 0.0 6 681 2 1 1 0 0 2 187 481 0.0 6 475 6 188 509 0.0 6 498 10 1 189 441 0.0 6 435 6 190 434 0.0 6 432 2 191 368 0.0 6 363 4 0 0 0 0 1 192 418 0.0 6 410 7 0 0 0 1 193 281 0.0 6 276 4 0 0 0 0 1 194 302 0.0 6 298 3 0 0 1 195 288 0.0 6 287 0 0 0 0 1 196 276 0.0 6 274 2 197 319 0.0 6 318 1 198 302 0.0 6 296 5 1 199 281 0.0 6 275 6 200 208 0.0 6 203 5 201 219 0.0 6 215 4 202 175 0.0 6 171 3 0 0 1 203 181 0.0 6 178 3 204 161 0.0 6 158 3 205 135 0.0 6 134 0 0 0 0 1 206 147 0.0 6 145 2 207 122 0.0 6 121 1 208 105 0.0 6 102 2 1 209 106 0.0 6 103 3 210 107 0.0 6 104 3 211 87 0.0 6 85 2 212 78 0.0 6 78 213 75 0.0 6 74 1 214 84 0.0 6 82 2 215 106 0.0 6 105 1 216 78 0.0 6 77 1 217 60 0.0 6 59 1 218 74 0.0 6 74 219 50 0.0 6 48 1 0 1 220 43 0.0 6 43 221 43 0.0 6 43 222 46 0.0 6 45 0 1 223 51 0.0 6 51 224 40 0.0 6 40 225 20 0.0 6 19 1 226 24 0.0 6 22 1 1 227 28 0.0 6 27 1 228 19 0.0 6 19 229 22 0.0 6 22 230 23 0.0 6 22 1 231 21 0.0 6 21 232 11 0.0 6 11 233 23 0.0 6 23 234 6 0.0 6 6 235 7 0.0 6 7 236 3 0.0 6 3 237 6 0.0 6 6 238 2 0.0 6 2 239 4 0.0 6 3 1 240 5 0.0 6 5 241 5 0.0 6 5 242 8 0.0 6 8 243 3 0.0 6 3 244 1 0.0 6 1 245 4 0.0 6 4 246 4 0.0 6 4 247 2 0.0 6 2 248 3 0.0 6 1 2 249 2 0.0 6 2 250 1 0.0 6 1 251 1 0.0 6 1 252 4 0.0 6 4 253 2 0.0 6 1 1 278 2 0.0 6 2 279 12 0.0 6 12 280 94 0.0 6 93 0 1 281 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1068 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 3 76 981.3 0 76 51 1 0.0 6 1 52 5 0.0 6 4 1 53 13 0.0 6 13 57 2 0.0 6 2 61 3 0.0 6 3 65 1 0.0 6 1 66 1 0.0 6 1 67 11 0.0 6 11 68 2 0.0 6 2 69 2 0.0 6 2 70 5 0.0 6 5 71 5 0.0 6 5 72 5 0.0 6 4 1 73 11 0.0 6 11 74 10 0.0 6 10 75 5 0.0 6 5 76 9 0.0 6 9 77 16 0.0 6 16 78 13 0.0 6 12 0 0 0 1 79 12 0.0 6 12 80 7 0.0 6 7 81 15 0.0 6 15 82 11 0.0 6 11 83 4 0.0 6 4 84 2 0.0 6 2 85 6 0.0 6 6 86 3 0.0 6 3 87 6 0.0 6 6 88 6 0.0 6 6 89 6 0.0 6 6 90 4 0.0 6 4 91 4 0.0 6 4 92 8 0.0 6 8 93 8 0.0 6 8 94 7 0.0 6 7 95 8 0.0 6 8 96 6 0.0 6 6 97 1 0.0 6 1 98 4 0.0 6 4 99 2 0.0 6 2 100 4 0.0 6 4 101 6 0.0 6 6 102 6 0.0 6 6 103 4 0.0 6 4 104 8 0.0 6 7 1 105 5 0.0 6 5 106 4 0.0 6 4 107 5 0.0 6 5 108 6 0.0 6 6 109 22 0.0 6 22 110 5 0.0 6 5 111 3 0.0 6 3 112 7 0.0 6 7 113 8 0.0 6 8 114 3 0.0 6 3 115 5 0.0 6 5 116 4 0.0 6 4 117 5 0.0 6 5 118 6 0.0 6 6 119 1 0.0 6 1 120 5 0.0 6 5 121 5 0.0 6 5 122 7 0.0 6 7 123 10 0.0 6 10 124 6 0.0 6 6 125 8 0.0 6 8 126 4 0.0 6 4 127 12 0.0 6 12 128 7 0.0 6 7 129 6 0.0 6 6 130 6 0.0 6 6 131 2 0.0 6 1 0 0 0 1 132 9 0.0 6 9 133 6 0.0 6 6 134 3 0.0 6 3 135 7 0.0 6 7 136 6 0.0 6 6 137 2 0.0 6 2 138 8 0.0 6 8 139 7 0.0 6 7 140 5 0.0 6 4 0 0 0 0 0 1 141 3 0.0 6 3 142 13 0.0 6 13 143 9 0.0 6 9 144 7 0.0 6 7 145 3 0.0 6 3 146 11 0.0 6 11 147 3 0.0 6 3 148 6 0.0 6 6 149 10 0.0 6 10 150 7 0.0 6 7 151 6 0.0 6 6 152 9 0.0 6 8 1 153 10 0.0 6 10 154 4 0.0 6 4 155 10 0.0 6 10 156 8 0.0 6 8 157 10 0.0 6 10 158 5 0.0 6 5 159 6 0.0 6 6 160 4 0.0 6 4 161 13 0.0 6 13 162 6 0.0 6 6 163 11 0.0 6 11 164 11 0.0 6 11 165 12 0.0 6 12 166 12 0.0 6 12 167 6 0.0 6 6 168 11 0.0 6 11 169 12 0.0 6 12 170 5 0.0 6 4 1 171 6 0.0 6 5 1 172 10 0.0 6 10 173 9 0.0 6 9 174 10 0.0 6 10 175 4 0.0 6 4 176 10 0.0 6 10 177 7 0.0 6 7 178 3 0.0 6 3 179 4 0.0 6 4 180 11 0.0 6 11 181 7 0.0 6 7 182 7 0.0 6 7 183 5 0.0 6 5 184 10 0.0 6 10 185 7 0.0 6 7 186 5 0.0 6 5 187 13 0.0 6 13 188 7 0.0 6 7 189 2 0.0 6 2 190 4 0.0 6 4 191 7 0.0 6 7 192 7 0.0 6 7 193 3 0.0 6 2 1 194 1 0.0 6 1 195 5 0.0 6 4 1 196 10 0.0 6 10 197 1 0.0 6 1 198 4 0.0 6 4 199 8 0.0 6 8 200 5 0.0 6 5 201 1 0.0 6 1 202 8 0.0 6 8 203 1 0.0 6 1 204 2 0.0 6 2 205 4 0.0 6 4 206 3 0.0 6 3 207 5 0.0 6 5 208 4 0.0 6 4 209 3 0.0 6 3 210 1 0.0 6 1 211 3 0.0 6 3 212 2 0.0 6 2 213 2 0.0 6 2 214 1 0.0 6 1 216 1 0.0 6 1 217 1 0.0 6 1 218 1 0.0 6 1 219 3 0.0 6 3 221 1 0.0 6 1 224 1 0.0 6 1 225 1 0.0 6 1 227 1 0.0 6 1 228 1 0.0 6 1 230 1 0.0 6 0 1 231 1 0.0 6 1 233 1 0.0 6 1 235 1 0.0 6 1 238 1 0.0 6 1 239 1 0.0 6 1 241 1 0.0 6 1 244 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_03_14_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_03_241_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_03_14_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_03_241_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.66 s (21 µs/read; 2.81 M reads/minute). === Summary === Total read pairs processed: 77,994 Read 1 with adapter: 77,988 (100.0%) Read 2 with adapter: 77,915 (99.9%) == Read fate breakdown == Pairs that were too short: 45 (0.1%) Pairs discarded as untrimmed: 85 (0.1%) Pairs written (passing filters): 77,864 (99.8%) Total basepairs processed: 34,110,758 bp Read 1: 19,359,991 bp Read 2: 14,750,767 bp Total written (filtered): 5,103,294 bp (15.0%) Read 1: 2,583,544 bp Read 2: 2,519,750 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 75972 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 1218.7 0 1 3 4 2 304.7 1 0 2 5 2 76.2 1 2 6 1 19.0 1 0 0 1 7 4 4.8 2 1 0 3 8 1 1.2 2 1 11 2 0.0 3 2 14 2 0.0 4 2 15 1 0.0 4 0 1 17 4 0.0 5 4 18 1 0.0 5 0 0 1 19 6 0.0 5 4 2 20 100 0.0 6 97 3 21 55 0.0 6 54 1 22 53 0.0 6 53 23 28 0.0 6 26 2 24 28 0.0 6 28 25 18 0.0 6 16 0 0 2 26 19 0.0 6 19 27 20 0.0 6 18 1 0 0 0 1 28 14 0.0 6 13 0 1 29 12 0.0 6 10 2 30 7 0.0 6 7 31 8 0.0 6 7 0 0 1 32 5 0.0 6 4 1 33 8 0.0 6 8 34 6 0.0 6 5 1 35 2 0.0 6 2 36 3 0.0 6 3 37 9 0.0 6 8 1 38 6 0.0 6 6 39 9 0.0 6 8 1 40 10 0.0 6 9 0 0 0 0 0 1 41 4 0.0 6 4 42 7 0.0 6 7 43 8 0.0 6 8 44 2 0.0 6 1 1 45 5 0.0 6 5 46 10 0.0 6 8 1 0 0 0 1 48 4 0.0 6 2 1 1 49 7 0.0 6 7 50 2 0.0 6 1 1 51 6 0.0 6 6 52 5 0.0 6 4 1 53 4 0.0 6 4 54 6 0.0 6 5 1 55 7 0.0 6 6 1 56 8 0.0 6 8 57 5 0.0 6 5 58 7 0.0 6 5 1 1 59 4 0.0 6 3 0 0 0 0 0 1 60 6 0.0 6 5 1 61 6 0.0 6 6 62 6 0.0 6 6 63 7 0.0 6 7 64 6 0.0 6 5 1 65 9 0.0 6 9 66 14 0.0 6 13 0 0 0 0 0 1 67 3 0.0 6 3 68 5 0.0 6 5 69 8 0.0 6 7 1 70 7 0.0 6 7 71 11 0.0 6 10 0 0 0 0 1 72 12 0.0 6 12 73 8 0.0 6 8 74 16 0.0 6 16 75 8 0.0 6 8 76 7 0.0 6 7 77 11 0.0 6 11 78 8 0.0 6 8 79 13 0.0 6 13 80 12 0.0 6 12 81 11 0.0 6 11 82 13 0.0 6 12 1 83 12 0.0 6 11 1 84 14 0.0 6 13 1 85 16 0.0 6 16 86 11 0.0 6 11 87 10 0.0 6 9 1 88 10 0.0 6 10 89 12 0.0 6 12 90 11 0.0 6 9 1 0 0 0 0 1 91 7 0.0 6 7 92 8 0.0 6 7 1 93 12 0.0 6 11 1 94 12 0.0 6 12 95 13 0.0 6 12 1 96 9 0.0 6 9 97 12 0.0 6 12 98 6 0.0 6 6 99 13 0.0 6 10 3 100 13 0.0 6 12 1 101 11 0.0 6 10 1 102 16 0.0 6 14 2 103 11 0.0 6 11 104 18 0.0 6 16 2 105 27 0.0 6 26 1 106 14 0.0 6 14 107 17 0.0 6 15 2 108 11 0.0 6 11 109 16 0.0 6 15 1 110 11 0.0 6 9 1 0 0 0 1 111 16 0.0 6 16 112 11 0.0 6 11 113 26 0.0 6 24 1 0 0 0 0 1 114 24 0.0 6 23 1 115 25 0.0 6 22 3 116 12 0.0 6 12 117 19 0.0 6 18 1 118 29 0.0 6 29 119 22 0.0 6 20 1 0 0 0 0 1 120 30 0.0 6 28 0 0 0 0 2 121 36 0.0 6 33 1 0 0 0 2 122 22 0.0 6 22 123 26 0.0 6 25 1 124 23 0.0 6 22 0 0 0 0 0 1 125 23 0.0 6 23 126 17 0.0 6 17 127 21 0.0 6 21 128 26 0.0 6 26 129 33 0.0 6 33 130 44 0.0 6 43 1 131 35 0.0 6 35 132 26 0.0 6 26 133 31 0.0 6 30 1 134 35 0.0 6 35 135 41 0.0 6 40 1 136 25 0.0 6 24 1 137 32 0.0 6 32 138 40 0.0 6 38 2 139 46 0.0 6 45 1 140 54 0.0 6 51 3 141 43 0.0 6 41 2 142 76 0.0 6 76 143 176 0.0 6 174 2 144 234 0.0 6 234 145 263 0.0 6 262 1 146 261 0.0 6 260 1 147 294 0.0 6 294 148 397 0.0 6 396 1 149 331 0.0 6 329 1 1 150 348 0.0 6 346 2 151 334 0.0 6 331 3 152 306 0.0 6 306 153 389 0.0 6 387 2 154 266 0.0 6 265 1 155 234 0.0 6 233 1 156 249 0.0 6 247 1 1 157 232 0.0 6 229 3 158 206 0.0 6 206 159 187 0.0 6 187 160 230 0.0 6 229 1 161 213 0.0 6 211 1 0 0 0 1 162 269 0.0 6 268 1 163 265 0.0 6 263 2 164 283 0.0 6 283 165 257 0.0 6 253 4 166 263 0.0 6 261 2 167 240 0.0 6 237 3 168 259 0.0 6 256 3 169 257 0.0 6 253 4 170 231 0.0 6 227 4 171 240 0.0 6 238 2 172 267 0.0 6 264 3 173 255 0.0 6 251 2 2 174 312 0.0 6 310 2 175 353 0.0 6 348 4 0 0 0 1 176 298 0.0 6 296 2 177 261 0.0 6 260 1 178 253 0.0 6 248 3 0 0 0 1 1 179 325 0.0 6 320 5 180 268 0.0 6 266 2 181 619 0.0 6 615 4 182 381 0.0 6 377 4 183 371 0.0 6 368 3 184 413 0.0 6 412 1 185 367 0.0 6 365 2 186 302 0.0 6 298 3 1 187 331 0.0 6 326 5 188 324 0.0 6 323 1 189 354 0.0 6 352 2 190 376 0.0 6 376 191 328 0.0 6 324 3 0 0 0 1 192 400 0.0 6 397 2 0 0 0 1 193 452 0.0 6 448 3 1 194 488 0.0 6 484 2 0 0 0 1 1 195 655 0.0 6 647 8 196 711 0.0 6 707 3 0 1 197 762 0.0 6 755 6 0 0 1 198 751 0.0 6 744 7 199 684 0.0 6 676 8 200 623 0.0 6 616 5 1 1 201 637 0.0 6 628 8 1 202 619 0.0 6 612 7 203 673 0.0 6 667 5 0 0 0 1 204 685 0.0 6 679 6 205 998 0.0 6 991 7 206 910 0.0 6 898 10 0 0 0 2 207 822 0.0 6 815 6 0 1 208 879 0.0 6 876 3 209 670 0.0 6 664 5 1 210 739 0.0 6 735 3 0 0 0 1 211 918 0.0 6 908 9 0 1 212 973 0.0 6 967 5 0 0 0 1 213 914 0.0 6 908 5 0 0 1 214 984 0.0 6 978 5 0 0 0 0 1 215 1292 0.0 6 1281 10 0 0 1 216 1011 0.0 6 1008 3 217 1002 0.0 6 992 10 218 1020 0.0 6 1011 7 0 0 0 1 1 219 930 0.0 6 927 2 0 0 1 220 823 0.0 6 817 5 0 0 1 221 739 0.0 6 736 3 222 764 0.0 6 762 2 223 878 0.0 6 874 4 224 789 0.0 6 785 4 225 978 0.0 6 970 8 226 955 0.0 6 949 6 227 910 0.0 6 903 6 0 0 0 1 228 834 0.0 6 831 3 229 840 0.0 6 837 3 230 820 0.0 6 820 231 857 0.0 6 845 9 2 0 0 0 1 232 843 0.0 6 838 3 0 0 1 1 233 839 0.0 6 835 3 1 234 796 0.0 6 790 6 235 687 0.0 6 682 5 236 704 0.0 6 700 3 0 0 1 237 708 0.0 6 704 3 0 1 238 745 0.0 6 741 4 239 743 0.0 6 738 3 1 0 0 1 240 737 0.0 6 735 1 0 0 1 241 761 0.0 6 760 1 242 626 0.0 6 622 3 0 0 0 1 243 669 0.0 6 664 5 244 586 0.0 6 581 4 0 0 0 1 245 536 0.0 6 534 2 246 491 0.0 6 485 4 1 0 1 247 496 0.0 6 495 1 248 462 0.0 6 461 1 249 470 0.0 6 469 1 250 432 0.0 6 427 4 1 251 473 0.0 6 473 252 402 0.0 6 400 2 253 415 0.0 6 415 254 379 0.0 6 379 255 365 0.0 6 362 3 256 386 0.0 6 383 3 257 377 0.0 6 376 1 258 363 0.0 6 360 3 259 470 0.0 6 466 4 260 390 0.0 6 386 4 261 277 0.0 6 275 0 0 0 0 1 1 262 307 0.0 6 305 2 263 335 0.0 6 335 264 290 0.0 6 289 1 265 260 0.0 6 259 1 266 268 0.0 6 267 1 267 284 0.0 6 283 1 268 260 0.0 6 258 2 269 345 0.0 6 342 3 270 241 0.0 6 241 271 242 0.0 6 241 1 272 234 0.0 6 232 2 273 334 0.0 6 334 274 402 0.0 6 397 4 1 275 414 0.0 6 410 4 276 573 0.0 6 570 3 277 635 0.0 6 633 0 0 0 0 2 278 2209 0.0 6 2192 15 2 279 2744 0.0 6 2720 23 0 0 0 1 280 21 0.0 6 21 281 3 0.0 6 3 283 1 0.0 6 1 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2016 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 3 0.0 6 0 2 1 63 108 0.0 6 106 2 64 1 0.0 6 1 65 1 0.0 6 0 1 71 1 0.0 6 1 72 6 0.0 6 6 73 12 0.0 6 12 74 11 0.0 6 11 75 11 0.0 6 11 76 9 0.0 6 9 77 12 0.0 6 12 78 6 0.0 6 5 1 79 9 0.0 6 9 80 8 0.0 6 8 81 18 0.0 6 18 82 15 0.0 6 15 83 4 0.0 6 4 84 5 0.0 6 5 85 6 0.0 6 6 86 4 0.0 6 4 87 2 0.0 6 2 88 2 0.0 6 2 89 5 0.0 6 5 90 5 0.0 6 5 91 7 0.0 6 7 92 4 0.0 6 4 93 9 0.0 6 9 94 2 0.0 6 2 95 3 0.0 6 3 96 4 0.0 6 4 97 7 0.0 6 7 98 4 0.0 6 4 99 4 0.0 6 4 100 2 0.0 6 2 101 6 0.0 6 6 102 14 0.0 6 14 103 4 0.0 6 4 104 6 0.0 6 6 105 2 0.0 6 2 106 5 0.0 6 5 107 3 0.0 6 3 108 7 0.0 6 7 109 14 0.0 6 14 110 17 0.0 6 17 111 6 0.0 6 6 112 6 0.0 6 6 113 7 0.0 6 6 1 114 17 0.0 6 16 1 115 6 0.0 6 6 116 6 0.0 6 6 117 6 0.0 6 6 118 5 0.0 6 5 119 4 0.0 6 4 120 5 0.0 6 5 121 4 0.0 6 4 122 3 0.0 6 3 123 4 0.0 6 4 124 5 0.0 6 5 125 7 0.0 6 6 1 126 11 0.0 6 11 127 12 0.0 6 12 128 9 0.0 6 9 129 8 0.0 6 8 130 8 0.0 6 8 131 8 0.0 6 8 132 4 0.0 6 4 133 14 0.0 6 14 134 11 0.0 6 11 135 5 0.0 6 5 136 7 0.0 6 7 137 7 0.0 6 7 138 10 0.0 6 10 139 13 0.0 6 13 140 12 0.0 6 12 141 10 0.0 6 9 1 142 11 0.0 6 11 143 10 0.0 6 10 144 12 0.0 6 12 145 7 0.0 6 7 146 10 0.0 6 10 147 12 0.0 6 12 148 9 0.0 6 9 149 9 0.0 6 9 150 14 0.0 6 14 151 11 0.0 6 11 152 18 0.0 6 18 153 7 0.0 6 7 154 13 0.0 6 13 155 9 0.0 6 8 1 156 5 0.0 6 5 157 14 0.0 6 14 158 27 0.0 6 27 159 11 0.0 6 11 160 5 0.0 6 5 161 11 0.0 6 11 162 14 0.0 6 14 163 12 0.0 6 12 164 15 0.0 6 15 165 11 0.0 6 11 166 17 0.0 6 17 167 28 0.0 6 28 168 19 0.0 6 19 169 15 0.0 6 15 170 18 0.0 6 18 171 19 0.0 6 19 172 14 0.0 6 14 173 18 0.0 6 18 174 21 0.0 6 21 175 11 0.0 6 11 176 10 0.0 6 10 177 16 0.0 6 16 178 14 0.0 6 14 179 15 0.0 6 15 180 16 0.0 6 16 181 14 0.0 6 14 182 15 0.0 6 15 183 12 0.0 6 12 184 15 0.0 6 14 1 185 8 0.0 6 8 186 9 0.0 6 9 187 12 0.0 6 12 188 19 0.0 6 19 189 19 0.0 6 19 190 15 0.0 6 15 191 17 0.0 6 17 192 19 0.0 6 19 193 12 0.0 6 12 194 13 0.0 6 13 195 13 0.0 6 13 196 16 0.0 6 16 197 12 0.0 6 12 198 15 0.0 6 15 199 9 0.0 6 9 200 15 0.0 6 15 201 22 0.0 6 22 202 12 0.0 6 12 203 26 0.0 6 26 204 8 0.0 6 8 205 12 0.0 6 12 206 10 0.0 6 10 207 7 0.0 6 7 208 6 0.0 6 6 209 17 0.0 6 17 210 5 0.0 6 5 211 11 0.0 6 11 212 20 0.0 6 20 213 14 0.0 6 14 214 10 0.0 6 10 215 7 0.0 6 7 216 11 0.0 6 11 217 14 0.0 6 14 218 17 0.0 6 17 219 6 0.0 6 6 220 8 0.0 6 8 221 8 0.0 6 8 222 9 0.0 6 9 223 12 0.0 6 12 224 12 0.0 6 12 225 9 0.0 6 9 226 10 0.0 6 10 227 4 0.0 6 4 228 5 0.0 6 5 229 8 0.0 6 8 230 10 0.0 6 10 231 4 0.0 6 4 232 5 0.0 6 5 233 7 0.0 6 7 234 8 0.0 6 8 235 11 0.0 6 11 236 8 0.0 6 8 237 12 0.0 6 12 238 7 0.0 6 7 239 7 0.0 6 7 240 8 0.0 6 8 241 9 0.0 6 9 242 8 0.0 6 8 243 4 0.0 6 4 244 6 0.0 6 6 245 4 0.0 6 4 246 6 0.0 6 6 247 9 0.0 6 9 248 10 0.0 6 10 249 14 0.0 6 14 250 7 0.0 6 7 251 2 0.0 6 2 252 4 0.0 6 4 253 8 0.0 6 8 254 6 0.0 6 6 255 1 0.0 6 1 256 2 0.0 6 2 257 2 0.0 6 2 258 4 0.0 6 4 259 3 0.0 6 3 260 5 0.0 6 5 261 3 0.0 6 3 262 7 0.0 6 7 263 1 0.0 6 1 264 3 0.0 6 3 265 2 0.0 6 2 266 2 0.0 6 2 267 3 0.0 6 3 268 4 0.0 6 4 269 1 0.0 6 1 272 3 0.0 6 3 273 1 0.0 6 1 274 1 0.0 6 1 275 1 0.0 6 1 276 3 0.0 6 3 277 4 0.0 6 4 278 2 0.0 6 2 280 2 0.0 6 2 281 4 0.0 6 4 282 1 0.0 6 1 283 1 0.0 6 1 286 1 0.0 6 1 287 1 0.0 6 1 288 1 0.0 6 1 292 3 0.0 6 3 295 1 0.0 6 0 0 0 0 0 0 1 296 1 0.0 6 1 298 1 0.0 6 1 299 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 76563 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1218.7 0 1 1 8 1 1.2 2 1 10 11 0.1 3 9 2 11 5 0.0 3 3 1 1 12 1 0.0 3 0 1 13 10 0.0 3 10 14 10 0.0 4 8 2 15 2 0.0 4 1 1 16 14 0.0 4 13 1 17 33 0.0 5 30 3 18 26 0.0 5 24 2 19 24 0.0 5 22 2 20 140 0.0 6 140 21 100 0.0 6 95 5 22 59 0.0 6 59 23 39 0.0 6 39 24 31 0.0 6 29 0 1 0 1 25 30 0.0 6 26 2 2 26 22 0.0 6 20 2 27 16 0.0 6 16 28 33 0.0 6 30 2 1 29 18 0.0 6 17 1 30 29 0.0 6 29 31 18 0.0 6 16 2 32 13 0.0 6 11 2 33 13 0.0 6 13 34 18 0.0 6 16 2 35 13 0.0 6 13 36 29 0.0 6 26 3 37 12 0.0 6 11 1 38 18 0.0 6 16 2 39 11 0.0 6 11 40 20 0.0 6 19 1 41 16 0.0 6 14 2 42 23 0.0 6 21 2 43 16 0.0 6 14 2 44 25 0.0 6 23 2 45 25 0.0 6 22 3 46 27 0.0 6 24 1 1 0 0 1 47 16 0.0 6 15 0 0 0 0 0 1 48 26 0.0 6 23 2 1 49 25 0.0 6 24 1 50 17 0.0 6 16 1 51 30 0.0 6 30 52 22 0.0 6 19 3 53 22 0.0 6 20 2 54 22 0.0 6 21 1 55 20 0.0 6 19 0 1 56 35 0.0 6 33 2 57 26 0.0 6 23 3 58 32 0.0 6 28 2 2 59 24 0.0 6 22 1 0 0 1 60 40 0.0 6 38 2 61 28 0.0 6 27 1 62 31 0.0 6 29 2 63 46 0.0 6 39 6 0 0 0 0 1 64 44 0.0 6 40 4 65 35 0.0 6 32 2 1 66 57 0.0 6 51 5 0 1 67 149 0.0 6 140 4 4 0 1 68 45 0.0 6 36 8 1 69 82 0.0 6 74 8 70 67 0.0 6 60 5 1 0 1 71 60 0.0 6 56 4 72 31 0.0 6 26 4 1 73 38 0.0 6 34 3 0 0 0 0 1 74 42 0.0 6 39 2 0 1 75 38 0.0 6 35 3 76 48 0.0 6 47 0 1 77 48 0.0 6 47 1 78 54 0.0 6 50 4 79 63 0.0 6 61 2 80 77 0.0 6 74 3 81 65 0.0 6 61 3 1 82 62 0.0 6 59 3 83 89 0.0 6 86 3 84 61 0.0 6 60 0 0 0 0 1 85 41 0.0 6 38 3 86 48 0.0 6 46 2 87 74 0.0 6 70 4 88 58 0.0 6 56 2 89 64 0.0 6 61 2 0 0 1 90 71 0.0 6 64 5 1 0 1 91 81 0.0 6 76 4 0 1 92 80 0.0 6 77 2 1 93 93 0.0 6 89 3 1 94 91 0.0 6 89 1 0 1 95 91 0.0 6 87 4 96 98 0.0 6 89 7 1 0 0 0 1 97 88 0.0 6 82 5 0 0 0 0 1 98 89 0.0 6 84 4 1 99 79 0.0 6 76 1 2 100 94 0.0 6 90 4 101 119 0.0 6 112 6 0 0 0 0 1 102 80 0.0 6 77 3 103 130 0.0 6 122 6 2 104 208 0.0 6 202 6 105 129 0.0 6 124 5 106 115 0.0 6 111 4 107 138 0.0 6 135 2 0 0 1 108 110 0.0 6 106 2 0 0 2 109 136 0.0 6 132 4 110 140 0.0 6 133 7 111 162 0.0 6 158 2 0 0 1 0 1 112 184 0.0 6 183 1 113 149 0.0 6 145 4 114 155 0.0 6 149 3 1 0 0 0 2 115 186 0.0 6 179 6 0 1 116 192 0.0 6 188 4 117 236 0.0 6 232 3 1 118 237 0.0 6 230 7 119 209 0.0 6 204 5 120 201 0.0 6 193 7 0 0 0 0 1 121 226 0.0 6 223 2 1 122 210 0.0 6 204 5 0 1 123 226 0.0 6 219 6 0 1 124 291 0.0 6 281 10 125 310 0.0 6 301 6 1 0 2 126 307 0.0 6 298 8 1 127 326 0.0 6 316 9 0 0 0 1 128 384 0.0 6 377 6 0 1 129 372 0.0 6 352 19 0 0 0 1 130 442 0.0 6 431 8 1 1 1 131 521 0.0 6 512 6 0 0 1 0 2 132 414 0.0 6 402 10 0 2 133 533 0.0 6 524 7 0 0 1 0 1 134 528 0.0 6 514 13 0 0 1 135 533 0.0 6 517 12 1 1 0 1 1 136 495 0.0 6 487 7 0 0 0 0 1 137 508 0.0 6 493 12 2 0 1 138 511 0.0 6 501 9 0 1 139 562 0.0 6 548 7 5 2 140 725 0.0 6 709 14 1 0 0 1 141 801 0.0 6 784 15 0 0 0 0 2 142 945 0.0 6 926 12 1 1 1 1 3 143 917 0.0 6 907 8 0 0 0 1 1 144 1020 0.0 6 1004 13 1 0 0 0 2 145 1088 0.0 6 1063 19 2 1 1 0 2 146 1091 0.0 6 1076 11 2 1 1 147 1120 0.0 6 1105 13 0 0 0 1 1 148 1353 0.0 6 1331 18 2 0 0 1 1 149 1344 0.0 6 1318 22 1 1 0 1 1 150 1736 0.0 6 1700 25 1 3 5 1 1 151 1389 0.0 6 1375 12 1 0 1 152 1564 0.0 6 1547 15 0 1 0 0 1 153 1536 0.0 6 1513 19 1 1 1 0 1 154 1271 0.0 6 1250 17 1 2 0 1 155 1334 0.0 6 1306 17 4 1 2 4 156 1224 0.0 6 1207 13 2 1 0 0 1 157 1405 0.0 6 1377 25 2 0 1 158 1308 0.0 6 1286 19 1 0 0 1 1 159 1262 0.0 6 1240 19 1 1 0 0 1 160 1211 0.0 6 1194 10 2 1 1 1 2 161 1108 0.0 6 1091 14 2 0 0 0 1 162 1005 0.0 6 990 12 1 0 0 2 163 1061 0.0 6 1043 14 1 0 1 2 164 1046 0.0 6 1025 19 0 0 0 1 1 165 993 0.0 6 984 7 0 0 0 1 1 166 1215 0.0 6 1194 16 1 1 0 2 1 167 939 0.0 6 934 3 0 0 2 168 1158 0.0 6 1143 12 0 2 0 0 1 169 998 0.0 6 987 7 0 2 0 1 1 170 1108 0.0 6 1087 19 0 1 0 0 1 171 1125 0.0 6 1118 7 172 1176 0.0 6 1155 19 0 1 0 1 173 1070 0.0 6 1061 8 1 174 989 0.0 6 983 6 175 980 0.0 6 963 13 1 1 1 0 1 176 1057 0.0 6 1050 7 177 923 0.0 6 906 16 0 0 0 1 178 872 0.0 6 866 5 0 1 179 689 0.0 6 685 4 180 691 0.0 6 684 6 0 0 0 1 181 673 0.0 6 667 2 0 1 0 3 182 650 0.0 6 643 4 2 1 183 921 0.0 6 910 10 0 0 0 0 1 184 1205 0.0 6 1193 11 0 0 1 185 1041 0.0 6 1036 4 0 0 0 1 186 730 0.0 6 721 7 0 0 1 1 187 647 0.0 6 640 6 0 0 0 1 188 632 0.0 6 624 8 189 581 0.0 6 573 8 190 583 0.0 6 576 6 0 0 0 1 191 626 0.0 6 618 7 1 192 432 0.0 6 430 2 193 416 0.0 6 411 4 0 0 0 1 194 445 0.0 6 441 3 1 195 360 0.0 6 356 2 1 1 196 531 0.0 6 522 8 1 197 390 0.0 6 384 5 0 0 0 0 1 198 369 0.0 6 362 7 199 377 0.0 6 373 4 200 320 0.0 6 310 9 0 0 0 1 201 257 0.0 6 256 1 202 276 0.0 6 270 4 1 0 1 203 236 0.0 6 234 1 0 0 1 204 217 0.0 6 213 4 205 195 0.0 6 190 3 0 1 1 206 194 0.0 6 191 3 207 191 0.0 6 186 4 1 208 180 0.0 6 179 1 209 163 0.0 6 162 1 210 125 0.0 6 125 211 109 0.0 6 109 212 152 0.0 6 147 5 213 132 0.0 6 132 214 143 0.0 6 143 215 108 0.0 6 106 2 216 121 0.0 6 119 2 217 92 0.0 6 90 2 218 96 0.0 6 94 1 0 1 219 90 0.0 6 87 3 220 35 0.0 6 35 221 84 0.0 6 83 0 1 222 69 0.0 6 69 223 81 0.0 6 78 3 224 56 0.0 6 56 225 46 0.0 6 45 1 226 45 0.0 6 45 227 34 0.0 6 34 228 31 0.0 6 31 229 31 0.0 6 30 1 230 27 0.0 6 26 1 231 27 0.0 6 27 232 21 0.0 6 21 233 19 0.0 6 19 234 7 0.0 6 7 235 11 0.0 6 10 1 236 8 0.0 6 8 237 2 0.0 6 2 238 8 0.0 6 8 239 7 0.0 6 7 240 7 0.0 6 7 241 14 0.0 6 14 242 15 0.0 6 15 243 10 0.0 6 9 1 244 11 0.0 6 11 245 4 0.0 6 4 246 8 0.0 6 8 247 4 0.0 6 3 1 248 8 0.0 6 8 249 2 0.0 6 2 250 1 0.0 6 1 251 2 0.0 6 2 252 1 0.0 6 1 253 1 0.0 6 1 277 5 0.0 6 5 278 18 0.0 6 18 279 125 0.0 6 122 2 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1352 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 49 4 0.0 6 0 4 52 1 0.0 6 1 54 1 0.0 6 1 57 1 0.0 6 1 59 2 0.0 6 2 61 1 0.0 6 1 63 1 0.0 6 1 69 2 0.0 6 2 71 2 0.0 6 2 72 4 0.0 6 4 73 7 0.0 6 7 74 10 0.0 6 10 75 10 0.0 6 10 76 6 0.0 6 6 77 19 0.0 6 19 78 18 0.0 6 18 79 12 0.0 6 12 80 18 0.0 6 18 81 9 0.0 6 9 82 20 0.0 6 20 83 7 0.0 6 7 84 10 0.0 6 10 85 6 0.0 6 6 86 14 0.0 6 14 87 7 0.0 6 7 88 3 0.0 6 3 89 6 0.0 6 6 90 8 0.0 6 8 91 5 0.0 6 5 92 15 0.0 6 15 93 5 0.0 6 5 94 8 0.0 6 8 95 8 0.0 6 8 96 6 0.0 6 6 97 10 0.0 6 10 98 3 0.0 6 3 99 5 0.0 6 5 100 4 0.0 6 4 101 5 0.0 6 5 102 6 0.0 6 5 0 0 0 1 103 11 0.0 6 11 104 8 0.0 6 8 105 14 0.0 6 14 106 4 0.0 6 4 107 3 0.0 6 3 108 2 0.0 6 2 109 7 0.0 6 7 110 24 0.0 6 24 111 6 0.0 6 6 112 11 0.0 6 11 113 13 0.0 6 13 114 8 0.0 6 8 115 7 0.0 6 7 116 6 0.0 6 5 0 0 1 117 5 0.0 6 5 118 3 0.0 6 3 119 3 0.0 6 3 120 3 0.0 6 3 121 2 0.0 6 2 122 4 0.0 6 4 123 5 0.0 6 5 124 6 0.0 6 6 125 10 0.0 6 10 126 12 0.0 6 12 127 6 0.0 6 6 128 7 0.0 6 7 129 11 0.0 6 11 130 6 0.0 6 6 131 16 0.0 6 16 132 2 0.0 6 2 133 10 0.0 6 10 134 19 0.0 6 19 135 9 0.0 6 9 136 4 0.0 6 4 137 11 0.0 6 10 1 138 10 0.0 6 10 139 9 0.0 6 9 140 6 0.0 6 6 141 5 0.0 6 5 142 8 0.0 6 7 1 143 13 0.0 6 13 144 10 0.0 6 10 145 10 0.0 6 9 1 146 7 0.0 6 7 147 12 0.0 6 12 148 11 0.0 6 11 149 9 0.0 6 9 150 9 0.0 6 8 0 0 0 1 151 18 0.0 6 18 152 11 0.0 6 11 153 12 0.0 6 12 154 16 0.0 6 16 155 14 0.0 6 14 156 19 0.0 6 19 157 15 0.0 6 15 158 22 0.0 6 22 159 10 0.0 6 10 160 8 0.0 6 8 161 9 0.0 6 8 1 162 13 0.0 6 13 163 15 0.0 6 15 164 21 0.0 6 21 165 29 0.0 6 29 166 16 0.0 6 16 167 16 0.0 6 16 168 14 0.0 6 14 169 10 0.0 6 10 170 19 0.0 6 19 171 14 0.0 6 14 172 4 0.0 6 4 173 14 0.0 6 13 1 174 14 0.0 6 14 175 14 0.0 6 14 176 11 0.0 6 11 177 8 0.0 6 8 178 12 0.0 6 12 179 10 0.0 6 10 180 17 0.0 6 17 181 10 0.0 6 10 182 11 0.0 6 11 183 8 0.0 6 8 184 5 0.0 6 5 185 5 0.0 6 5 186 8 0.0 6 8 187 8 0.0 6 8 188 8 0.0 6 8 189 9 0.0 6 9 190 11 0.0 6 11 191 10 0.0 6 10 192 13 0.0 6 13 193 9 0.0 6 9 194 3 0.0 6 3 195 9 0.0 6 9 196 9 0.0 6 9 197 3 0.0 6 3 198 6 0.0 6 5 1 199 5 0.0 6 5 200 6 0.0 6 6 201 7 0.0 6 7 202 3 0.0 6 3 203 9 0.0 6 9 204 4 0.0 6 4 205 6 0.0 6 6 206 4 0.0 6 4 207 2 0.0 6 2 209 3 0.0 6 3 210 4 0.0 6 4 211 4 0.0 6 4 212 1 0.0 6 1 213 2 0.0 6 2 214 4 0.0 6 4 215 2 0.0 6 1 0 0 0 1 218 2 0.0 6 2 219 1 0.0 6 1 220 1 0.0 6 1 221 2 0.0 6 2 222 3 0.0 6 3 223 3 0.0 6 3 227 1 0.0 6 0 1 228 1 0.0 6 1 229 3 0.0 6 3 230 2 0.0 6 2 236 2 0.0 6 2 237 1 0.0 6 1 239 2 0.0 6 2 248 1 0.0 6 1 249 2 0.0 6 2 250 2 0.0 6 2 253 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_04_15_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_04_242_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_04_15_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_04_242_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.97 s (155 µs/read; 0.39 M reads/minute). === Summary === Total read pairs processed: 6,246 Read 1 with adapter: 6,245 (100.0%) Read 2 with adapter: 6,241 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 5 (0.1%) Pairs written (passing filters): 6,241 (99.9%) Total basepairs processed: 3,126,654 bp Read 1: 1,762,497 bp Read 2: 1,364,157 bp Total written (filtered): 381,671 bp (12.2%) Read 1: 200,026 bp Read 2: 181,645 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 6239 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.8% G: 0.0% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 36 1 0.0 6 1 40 1 0.0 6 1 50 1 0.0 6 1 63 1 0.0 6 1 72 1 0.0 6 1 73 1 0.0 6 1 77 1 0.0 6 1 78 1 0.0 6 1 86 2 0.0 6 2 92 1 0.0 6 1 94 1 0.0 6 1 98 2 0.0 6 2 104 1 0.0 6 1 106 2 0.0 6 2 108 1 0.0 6 1 109 2 0.0 6 2 110 1 0.0 6 1 111 1 0.0 6 1 112 1 0.0 6 1 113 4 0.0 6 4 114 1 0.0 6 1 115 1 0.0 6 1 116 1 0.0 6 1 117 1 0.0 6 1 119 1 0.0 6 1 124 3 0.0 6 1 2 125 1 0.0 6 1 126 1 0.0 6 1 127 1 0.0 6 0 1 128 1 0.0 6 1 130 3 0.0 6 3 132 1 0.0 6 1 134 4 0.0 6 3 1 135 2 0.0 6 2 137 3 0.0 6 3 138 3 0.0 6 3 139 2 0.0 6 2 140 4 0.0 6 4 141 1 0.0 6 1 142 3 0.0 6 3 143 3 0.0 6 3 144 5 0.0 6 5 145 9 0.0 6 9 146 155 0.0 6 155 147 17 0.0 6 17 148 2 0.0 6 2 149 2 0.0 6 2 150 5 0.0 6 5 151 1 0.0 6 1 152 3 0.0 6 2 1 154 1 0.0 6 1 155 1 0.0 6 1 157 2 0.0 6 1 1 158 2 0.0 6 2 160 7 0.0 6 7 161 3 0.0 6 3 162 2 0.0 6 1 1 163 1 0.0 6 1 164 5 0.0 6 5 165 2 0.0 6 1 1 166 1 0.0 6 1 167 6 0.0 6 6 168 7 0.0 6 7 169 1 0.0 6 1 170 2 0.0 6 2 171 7 0.0 6 7 172 3 0.0 6 3 173 1 0.0 6 0 1 174 2 0.0 6 1 1 175 3 0.0 6 3 176 4 0.0 6 3 1 177 4 0.0 6 4 178 2 0.0 6 2 179 5 0.0 6 5 180 5 0.0 6 5 181 6 0.0 6 5 1 182 6 0.0 6 6 183 3 0.0 6 3 184 8 0.0 6 7 1 185 5 0.0 6 4 1 186 18 0.0 6 18 187 14 0.0 6 14 188 4 0.0 6 4 189 4 0.0 6 3 1 190 6 0.0 6 6 191 11 0.0 6 10 1 192 11 0.0 6 11 193 14 0.0 6 13 1 194 11 0.0 6 10 1 195 25 0.0 6 23 1 1 196 16 0.0 6 16 197 8 0.0 6 7 1 198 20 0.0 6 20 199 17 0.0 6 17 200 19 0.0 6 18 1 201 22 0.0 6 21 1 202 11 0.0 6 10 1 203 12 0.0 6 12 204 19 0.0 6 18 0 1 205 16 0.0 6 16 206 23 0.0 6 22 1 207 22 0.0 6 22 208 32 0.0 6 32 209 26 0.0 6 26 210 18 0.0 6 18 211 39 0.0 6 39 212 9 0.0 6 8 1 213 38 0.0 6 33 5 214 21 0.0 6 20 1 215 14 0.0 6 14 216 14 0.0 6 12 2 217 26 0.0 6 25 1 218 17 0.0 6 17 219 20 0.0 6 20 220 17 0.0 6 16 1 221 29 0.0 6 27 2 222 29 0.0 6 29 223 38 0.0 6 36 2 224 28 0.0 6 27 1 225 42 0.0 6 41 1 226 54 0.0 6 54 227 35 0.0 6 32 3 228 33 0.0 6 33 229 31 0.0 6 29 2 230 39 0.0 6 37 2 231 23 0.0 6 23 232 19 0.0 6 19 233 31 0.0 6 29 2 234 29 0.0 6 29 235 21 0.0 6 21 236 22 0.0 6 22 237 55 0.0 6 54 1 238 29 0.0 6 29 239 33 0.0 6 32 1 240 40 0.0 6 36 3 1 241 35 0.0 6 34 1 242 20 0.0 6 20 243 56 0.0 6 54 2 244 57 0.0 6 55 2 245 51 0.0 6 49 2 246 17 0.0 6 17 247 32 0.0 6 29 1 2 248 37 0.0 6 35 1 1 249 114 0.0 6 106 8 250 54 0.0 6 48 6 251 45 0.0 6 45 252 77 0.0 6 76 1 253 82 0.0 6 78 4 254 98 0.0 6 93 5 255 65 0.0 6 62 3 256 142 0.0 6 139 3 257 66 0.0 6 59 6 1 258 172 0.0 6 168 4 259 113 0.0 6 108 5 260 198 0.0 6 188 9 1 261 35 0.0 6 34 1 262 42 0.0 6 39 3 263 74 0.0 6 73 1 264 68 0.0 6 67 1 265 34 0.0 6 32 2 266 78 0.0 6 75 3 267 57 0.0 6 54 2 1 268 79 0.0 6 77 2 269 75 0.0 6 71 4 270 38 0.0 6 37 0 1 271 64 0.0 6 63 1 272 82 0.0 6 80 2 273 132 0.0 6 128 4 274 116 0.0 6 112 3 1 275 221 0.0 6 218 3 276 270 0.0 6 262 8 277 291 0.0 6 285 5 1 278 1020 0.0 6 991 27 2 279 275 0.0 6 262 13 280 2 0.0 6 1 1 281 3 0.0 6 2 0 0 1 282 2 0.0 6 2 287 1 0.0 6 1 289 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 6 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 77 1 0.0 6 1 78 5 0.0 6 5 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 6229 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.8% G: 0.1% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 16 1 0.0 4 1 17 2 0.0 5 2 19 3 0.0 5 3 20 3 0.0 6 3 21 4 0.0 6 3 1 22 3 0.0 6 3 23 1 0.0 6 1 24 3 0.0 6 3 26 1 0.0 6 0 0 0 0 0 1 27 1 0.0 6 0 0 0 0 1 28 1 0.0 6 1 29 2 0.0 6 2 31 1 0.0 6 1 39 2 0.0 6 2 41 2 0.0 6 2 44 1 0.0 6 1 45 1 0.0 6 1 47 1 0.0 6 1 48 4 0.0 6 3 1 49 2 0.0 6 2 50 1 0.0 6 1 51 2 0.0 6 2 52 3 0.0 6 2 1 53 2 0.0 6 1 1 54 1 0.0 6 1 55 1 0.0 6 1 56 4 0.0 6 4 57 2 0.0 6 2 58 1 0.0 6 1 60 2 0.0 6 1 1 62 1 0.0 6 1 63 2 0.0 6 2 64 1 0.0 6 1 66 3 0.0 6 3 68 3 0.0 6 2 1 69 5 0.0 6 5 70 18 0.0 6 16 2 71 4 0.0 6 4 72 7 0.0 6 7 73 1 0.0 6 1 74 1 0.0 6 1 75 1 0.0 6 1 76 2 0.0 6 2 77 2 0.0 6 1 0 0 0 1 78 1 0.0 6 1 80 1 0.0 6 1 82 2 0.0 6 2 83 4 0.0 6 4 84 3 0.0 6 3 85 3 0.0 6 3 86 3 0.0 6 3 87 3 0.0 6 2 1 88 2 0.0 6 1 1 89 3 0.0 6 3 90 1 0.0 6 1 91 2 0.0 6 2 92 2 0.0 6 2 93 1 0.0 6 1 94 2 0.0 6 1 1 95 3 0.0 6 2 1 96 3 0.0 6 3 97 4 0.0 6 2 1 1 98 2 0.0 6 2 99 7 0.0 6 7 100 5 0.0 6 4 1 101 3 0.0 6 3 102 3 0.0 6 2 1 103 5 0.0 6 4 1 104 4 0.0 6 4 105 3 0.0 6 3 106 4 0.0 6 4 107 8 0.0 6 8 108 4 0.0 6 4 109 2 0.0 6 2 110 5 0.0 6 4 0 0 0 1 111 5 0.0 6 5 112 6 0.0 6 4 2 113 5 0.0 6 4 1 114 3 0.0 6 2 1 115 5 0.0 6 5 116 1 0.0 6 1 117 3 0.0 6 2 1 118 3 0.0 6 3 119 3 0.0 6 3 120 14 0.0 6 14 121 6 0.0 6 5 1 122 5 0.0 6 4 1 123 4 0.0 6 4 124 10 0.0 6 10 125 7 0.0 6 7 126 1 0.0 6 1 127 8 0.0 6 8 128 4 0.0 6 4 129 8 0.0 6 8 130 9 0.0 6 9 131 6 0.0 6 6 132 12 0.0 6 12 133 8 0.0 6 8 134 5 0.0 6 5 135 29 0.0 6 25 4 136 11 0.0 6 11 137 17 0.0 6 15 1 1 138 7 0.0 6 7 139 12 0.0 6 12 140 12 0.0 6 11 1 141 17 0.0 6 14 3 142 19 0.0 6 19 143 22 0.0 6 22 144 47 0.0 6 44 3 145 46 0.0 6 45 1 146 117 0.0 6 116 1 147 21 0.0 6 20 1 148 17 0.0 6 17 149 17 0.0 6 16 1 150 30 0.0 6 27 3 151 14 0.0 6 13 1 152 39 0.0 6 39 153 29 0.0 6 28 1 154 25 0.0 6 25 155 25 0.0 6 24 1 156 27 0.0 6 26 1 157 26 0.0 6 26 158 39 0.0 6 38 1 159 8 0.0 6 7 0 1 160 41 0.0 6 38 3 161 28 0.0 6 27 1 162 16 0.0 6 16 163 33 0.0 6 32 1 164 34 0.0 6 33 1 165 35 0.0 6 34 1 166 48 0.0 6 45 3 167 19 0.0 6 17 2 168 60 0.0 6 55 5 169 19 0.0 6 19 170 14 0.0 6 14 171 46 0.0 6 44 2 172 14 0.0 6 13 1 173 27 0.0 6 24 2 1 174 28 0.0 6 27 1 175 81 0.0 6 80 1 176 52 0.0 6 50 2 177 68 0.0 6 67 1 178 43 0.0 6 40 3 179 48 0.0 6 46 2 180 55 0.0 6 52 2 1 181 82 0.0 6 78 4 182 25 0.0 6 23 2 183 69 0.0 6 63 6 184 21 0.0 6 20 1 185 20 0.0 6 19 1 186 84 0.0 6 79 4 0 1 187 149 0.0 6 143 6 188 130 0.0 6 129 1 189 106 0.0 6 104 2 190 134 0.0 6 133 1 191 122 0.0 6 116 6 192 73 0.0 6 71 2 193 97 0.0 6 93 4 194 52 0.0 6 51 0 0 0 1 195 131 0.0 6 123 8 196 178 0.0 6 174 4 197 78 0.0 6 74 4 198 192 0.0 6 185 7 199 259 0.0 6 249 9 1 200 68 0.0 6 66 1 1 201 148 0.0 6 145 3 202 80 0.0 6 77 3 203 74 0.0 6 73 1 204 82 0.0 6 77 5 205 88 0.0 6 87 1 206 27 0.0 6 24 3 207 36 0.0 6 35 0 0 0 1 208 79 0.0 6 77 1 0 0 1 209 60 0.0 6 60 210 21 0.0 6 20 1 211 65 0.0 6 63 2 212 66 0.0 6 63 2 0 0 1 213 49 0.0 6 48 1 214 38 0.0 6 38 215 32 0.0 6 32 216 57 0.0 6 57 217 85 0.0 6 83 2 218 49 0.0 6 47 2 219 37 0.0 6 37 220 37 0.0 6 36 1 221 34 0.0 6 33 1 222 40 0.0 6 40 223 75 0.0 6 71 4 224 112 0.0 6 106 6 225 76 0.0 6 74 2 226 46 0.0 6 45 1 227 151 0.0 6 149 2 228 29 0.0 6 27 2 229 105 0.0 6 101 4 230 19 0.0 6 19 231 24 0.0 6 23 1 232 39 0.0 6 37 2 233 37 0.0 6 37 234 45 0.0 6 42 3 235 35 0.0 6 31 4 236 7 0.0 6 6 1 237 19 0.0 6 19 238 9 0.0 6 9 239 5 0.0 6 3 2 240 6 0.0 6 6 241 5 0.0 6 5 242 33 0.0 6 31 2 243 8 0.0 6 8 244 26 0.0 6 23 3 245 10 0.0 6 10 246 6 0.0 6 6 247 11 0.0 6 10 1 248 6 0.0 6 6 249 16 0.0 6 16 250 5 0.0 6 5 251 23 0.0 6 22 1 252 13 0.0 6 13 253 4 0.0 6 4 254 11 0.0 6 11 255 3 0.0 6 3 259 1 0.0 6 1 279 1 0.0 6 1 280 2 0.0 6 2 281 18 0.0 6 18 282 4 0.0 6 4 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 12 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 55 1 0.0 6 0 1 59 1 0.0 6 1 73 1 0.0 6 1 74 3 0.0 6 3 75 6 0.0 6 6 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_05_16_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_05_243_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_05_16_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_05_243_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.96 s (29 µs/read; 2.09 M reads/minute). === Summary === Total read pairs processed: 68,211 Read 1 with adapter: 68,204 (100.0%) Read 2 with adapter: 68,173 (99.9%) == Read fate breakdown == Pairs that were too short: 28 (0.0%) Pairs discarded as untrimmed: 45 (0.1%) Pairs written (passing filters): 68,138 (99.9%) Total basepairs processed: 29,267,943 bp Read 1: 16,413,474 bp Read 2: 12,854,469 bp Total written (filtered): 4,358,311 bp (14.9%) Read 1: 2,265,321 bp Read 2: 2,092,990 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 66518 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1065.8 0 0 1 4 4 266.4 1 2 2 5 1 66.6 1 1 6 1 16.7 1 0 0 1 7 6 4.2 2 6 8 2 1.0 2 2 10 1 0.1 3 1 15 4 0.0 4 2 2 16 1 0.0 4 1 17 1 0.0 5 1 19 2 0.0 5 1 1 20 73 0.0 6 71 2 21 45 0.0 6 44 0 0 1 22 42 0.0 6 41 1 23 31 0.0 6 30 1 24 14 0.0 6 14 25 16 0.0 6 16 26 8 0.0 6 7 1 27 9 0.0 6 8 1 28 8 0.0 6 7 1 29 17 0.0 6 17 30 3 0.0 6 3 31 5 0.0 6 5 32 5 0.0 6 5 33 3 0.0 6 3 34 5 0.0 6 4 1 36 9 0.0 6 8 0 1 37 5 0.0 6 5 38 1 0.0 6 1 39 11 0.0 6 11 40 6 0.0 6 5 0 0 1 41 5 0.0 6 4 1 42 5 0.0 6 5 43 2 0.0 6 2 44 7 0.0 6 6 1 45 7 0.0 6 6 0 0 0 0 1 46 4 0.0 6 4 47 7 0.0 6 7 48 5 0.0 6 4 0 1 49 6 0.0 6 5 1 50 4 0.0 6 3 1 51 7 0.0 6 7 52 3 0.0 6 3 53 9 0.0 6 9 54 5 0.0 6 5 55 2 0.0 6 2 56 1 0.0 6 1 58 2 0.0 6 2 59 4 0.0 6 4 60 2 0.0 6 2 61 5 0.0 6 5 62 5 0.0 6 3 2 63 5 0.0 6 4 1 64 9 0.0 6 7 1 0 0 0 1 65 11 0.0 6 11 66 6 0.0 6 6 67 4 0.0 6 4 68 12 0.0 6 12 69 11 0.0 6 11 70 7 0.0 6 7 71 16 0.0 6 16 72 5 0.0 6 5 73 6 0.0 6 6 74 15 0.0 6 14 1 75 5 0.0 6 5 76 5 0.0 6 5 77 4 0.0 6 4 78 6 0.0 6 6 79 3 0.0 6 3 80 8 0.0 6 8 81 8 0.0 6 8 82 6 0.0 6 6 83 7 0.0 6 7 84 11 0.0 6 11 85 7 0.0 6 6 1 86 8 0.0 6 8 87 8 0.0 6 8 88 7 0.0 6 7 89 7 0.0 6 7 90 8 0.0 6 8 91 7 0.0 6 6 1 92 11 0.0 6 10 1 93 5 0.0 6 4 1 94 6 0.0 6 5 1 95 8 0.0 6 8 96 10 0.0 6 10 97 3 0.0 6 3 98 9 0.0 6 9 99 6 0.0 6 6 100 9 0.0 6 9 101 13 0.0 6 12 1 102 12 0.0 6 12 103 16 0.0 6 16 104 22 0.0 6 22 105 13 0.0 6 13 106 15 0.0 6 15 107 14 0.0 6 13 1 108 16 0.0 6 16 109 22 0.0 6 22 110 20 0.0 6 20 111 27 0.0 6 27 112 23 0.0 6 23 113 25 0.0 6 25 114 25 0.0 6 25 115 18 0.0 6 18 116 25 0.0 6 25 117 14 0.0 6 14 118 21 0.0 6 21 119 24 0.0 6 23 1 120 15 0.0 6 14 0 0 0 0 1 121 29 0.0 6 29 122 22 0.0 6 22 123 23 0.0 6 22 1 124 24 0.0 6 23 1 125 26 0.0 6 24 1 0 0 0 0 1 126 32 0.0 6 32 127 21 0.0 6 21 128 27 0.0 6 27 129 49 0.0 6 48 1 130 31 0.0 6 31 131 36 0.0 6 35 1 132 28 0.0 6 28 133 35 0.0 6 34 1 134 48 0.0 6 47 1 135 41 0.0 6 38 2 0 0 0 0 1 136 47 0.0 6 47 137 37 0.0 6 37 138 45 0.0 6 45 139 56 0.0 6 55 1 140 93 0.0 6 92 1 141 247 0.0 6 245 2 142 409 0.0 6 406 2 1 143 520 0.0 6 518 2 144 445 0.0 6 443 2 145 482 0.0 6 479 3 146 640 0.0 6 637 2 1 147 624 0.0 6 622 2 148 777 0.0 6 772 5 149 608 0.0 6 604 4 150 630 0.0 6 629 1 151 978 0.0 6 976 2 152 522 0.0 6 522 153 336 0.0 6 334 2 154 355 0.0 6 355 155 355 0.0 6 354 1 156 320 0.0 6 320 157 294 0.0 6 293 1 158 285 0.0 6 284 1 159 367 0.0 6 367 160 430 0.0 6 428 2 161 384 0.0 6 383 1 162 397 0.0 6 396 1 163 321 0.0 6 320 1 164 359 0.0 6 358 0 0 0 0 0 1 165 252 0.0 6 250 1 1 166 233 0.0 6 229 3 1 167 307 0.0 6 305 2 168 310 0.0 6 309 1 169 302 0.0 6 299 3 170 327 0.0 6 324 3 171 351 0.0 6 348 2 1 172 357 0.0 6 356 1 173 350 0.0 6 347 3 174 380 0.0 6 378 2 175 257 0.0 6 255 2 176 285 0.0 6 282 2 1 177 238 0.0 6 234 4 178 257 0.0 6 257 179 515 0.0 6 510 3 0 1 1 180 307 0.0 6 307 181 319 0.0 6 314 4 1 182 306 0.0 6 305 1 183 329 0.0 6 323 5 0 0 0 1 184 280 0.0 6 276 3 0 0 0 1 185 309 0.0 6 307 2 186 347 0.0 6 344 3 187 345 0.0 6 343 2 188 329 0.0 6 328 1 189 312 0.0 6 311 1 190 317 0.0 6 316 0 0 0 0 1 191 372 0.0 6 367 5 192 364 0.0 6 361 3 193 392 0.0 6 386 5 0 0 1 194 461 0.0 6 457 4 195 507 0.0 6 501 6 196 597 0.0 6 589 6 0 0 0 2 197 653 0.0 6 648 4 1 198 675 0.0 6 673 2 199 530 0.0 6 527 3 200 493 0.0 6 492 1 201 537 0.0 6 536 1 202 481 0.0 6 479 2 203 583 0.0 6 575 7 0 0 0 0 1 204 553 0.0 6 547 5 0 0 1 205 544 0.0 6 542 2 206 758 0.0 6 749 8 1 207 636 0.0 6 630 5 0 1 208 633 0.0 6 628 4 0 0 1 209 443 0.0 6 441 1 1 210 496 0.0 6 491 4 0 0 0 1 211 610 0.0 6 607 3 212 692 0.0 6 685 5 1 0 1 213 652 0.0 6 651 1 214 736 0.0 6 724 12 215 875 0.0 6 868 6 1 216 679 0.0 6 676 3 217 612 0.0 6 603 8 0 0 1 218 678 0.0 6 671 7 219 671 0.0 6 668 3 220 524 0.0 6 518 6 221 549 0.0 6 546 2 0 1 222 554 0.0 6 550 3 1 223 651 0.0 6 648 3 224 651 0.0 6 645 6 225 735 0.0 6 730 5 226 705 0.0 6 704 1 227 604 0.0 6 603 1 228 546 0.0 6 542 4 229 467 0.0 6 464 3 230 566 0.0 6 561 5 231 551 0.0 6 548 3 232 570 0.0 6 564 6 233 503 0.0 6 501 2 234 534 0.0 6 529 4 0 0 0 1 235 422 0.0 6 419 3 236 473 0.0 6 467 6 237 491 0.0 6 480 10 0 0 1 238 510 0.0 6 504 5 1 239 591 0.0 6 588 2 0 1 240 499 0.0 6 494 4 0 0 0 1 241 522 0.0 6 517 5 242 528 0.0 6 523 4 1 243 397 0.0 6 395 2 244 374 0.0 6 371 2 0 1 245 380 0.0 6 377 3 246 382 0.0 6 376 5 0 0 0 0 1 247 334 0.0 6 332 2 248 363 0.0 6 358 4 1 249 362 0.0 6 360 2 250 349 0.0 6 346 2 1 251 388 0.0 6 385 3 252 279 0.0 6 277 2 253 310 0.0 6 306 4 254 291 0.0 6 286 5 255 301 0.0 6 299 2 256 294 0.0 6 291 3 257 328 0.0 6 325 3 258 278 0.0 6 278 259 358 0.0 6 355 3 260 392 0.0 6 389 3 261 229 0.0 6 226 3 262 247 0.0 6 246 1 263 233 0.0 6 232 0 0 1 264 207 0.0 6 207 265 221 0.0 6 216 5 266 264 0.0 6 257 6 1 267 214 0.0 6 212 2 268 227 0.0 6 224 3 269 334 0.0 6 328 6 270 234 0.0 6 232 2 271 233 0.0 6 231 2 272 167 0.0 6 166 1 273 272 0.0 6 272 274 426 0.0 6 419 7 275 302 0.0 6 298 4 276 325 0.0 6 318 7 277 515 0.0 6 506 8 0 0 1 278 2878 0.0 6 2828 47 1 0 0 0 2 279 1979 0.0 6 1941 37 0 1 280 18 0.0 6 15 3 281 7 0.0 6 5 0 2 282 2 0.0 6 2 283 2 0.0 6 2 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1686 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 51 1 0.0 6 0 1 54 1 0.0 6 1 62 11 0.0 6 2 9 63 193 0.0 6 188 5 64 1 0.0 6 1 65 5 0.0 6 5 67 1 0.0 6 1 71 8 0.0 6 8 72 11 0.0 6 11 73 19 0.0 6 19 74 13 0.0 6 13 75 19 0.0 6 19 76 15 0.0 6 15 77 16 0.0 6 16 78 20 0.0 6 20 79 28 0.0 6 28 80 17 0.0 6 17 81 32 0.0 6 32 82 27 0.0 6 27 83 16 0.0 6 16 84 7 0.0 6 7 85 5 0.0 6 5 86 13 0.0 6 13 87 7 0.0 6 7 88 7 0.0 6 6 1 89 9 0.0 6 9 90 14 0.0 6 14 91 17 0.0 6 17 92 14 0.0 6 14 93 9 0.0 6 9 94 8 0.0 6 8 95 6 0.0 6 6 96 3 0.0 6 3 97 11 0.0 6 11 98 9 0.0 6 9 99 10 0.0 6 10 100 6 0.0 6 6 101 11 0.0 6 11 102 9 0.0 6 9 103 16 0.0 6 16 104 12 0.0 6 12 105 5 0.0 6 5 106 5 0.0 6 5 107 10 0.0 6 10 108 5 0.0 6 5 109 8 0.0 6 8 110 17 0.0 6 17 111 11 0.0 6 11 112 10 0.0 6 10 113 5 0.0 6 5 114 4 0.0 6 4 115 4 0.0 6 4 116 1 0.0 6 1 117 6 0.0 6 6 118 5 0.0 6 5 119 11 0.0 6 11 120 4 0.0 6 4 121 6 0.0 6 6 122 1 0.0 6 1 123 2 0.0 6 2 124 6 0.0 6 6 125 4 0.0 6 4 126 8 0.0 6 8 127 13 0.0 6 13 128 11 0.0 6 11 129 3 0.0 6 3 130 4 0.0 6 4 131 11 0.0 6 11 132 5 0.0 6 5 133 9 0.0 6 9 134 6 0.0 6 6 135 6 0.0 6 6 136 8 0.0 6 8 137 6 0.0 6 6 138 9 0.0 6 9 139 8 0.0 6 8 140 3 0.0 6 3 141 3 0.0 6 3 142 6 0.0 6 6 143 3 0.0 6 3 144 7 0.0 6 7 145 6 0.0 6 6 146 2 0.0 6 2 147 12 0.0 6 12 148 3 0.0 6 3 149 5 0.0 6 5 150 10 0.0 6 10 151 14 0.0 6 14 152 10 0.0 6 9 0 1 153 8 0.0 6 8 154 4 0.0 6 4 155 6 0.0 6 6 156 5 0.0 6 5 157 8 0.0 6 8 158 6 0.0 6 6 159 8 0.0 6 8 160 5 0.0 6 5 161 6 0.0 6 6 162 9 0.0 6 9 163 3 0.0 6 3 164 12 0.0 6 12 165 4 0.0 6 4 166 9 0.0 6 9 167 7 0.0 6 7 168 9 0.0 6 9 169 11 0.0 6 11 170 5 0.0 6 5 171 5 0.0 6 5 172 4 0.0 6 4 173 7 0.0 6 7 174 7 0.0 6 7 175 11 0.0 6 11 176 8 0.0 6 8 177 6 0.0 6 6 178 9 0.0 6 9 179 4 0.0 6 4 180 4 0.0 6 4 181 1 0.0 6 1 182 2 0.0 6 2 183 3 0.0 6 3 184 3 0.0 6 3 185 11 0.0 6 11 186 9 0.0 6 9 187 6 0.0 6 6 188 12 0.0 6 12 189 8 0.0 6 8 190 8 0.0 6 8 191 7 0.0 6 7 192 10 0.0 6 10 193 11 0.0 6 11 194 9 0.0 6 9 195 5 0.0 6 5 196 4 0.0 6 4 197 5 0.0 6 5 198 9 0.0 6 9 199 11 0.0 6 11 200 9 0.0 6 9 201 10 0.0 6 10 202 10 0.0 6 10 203 11 0.0 6 11 204 8 0.0 6 8 205 3 0.0 6 3 206 4 0.0 6 4 207 3 0.0 6 3 208 7 0.0 6 7 209 8 0.0 6 8 210 7 0.0 6 7 211 6 0.0 6 6 212 12 0.0 6 12 213 7 0.0 6 7 214 8 0.0 6 8 215 5 0.0 6 5 216 5 0.0 6 5 217 6 0.0 6 6 218 6 0.0 6 6 219 2 0.0 6 2 220 11 0.0 6 11 221 11 0.0 6 11 222 4 0.0 6 4 223 6 0.0 6 6 224 8 0.0 6 8 225 8 0.0 6 8 226 10 0.0 6 10 227 10 0.0 6 10 228 4 0.0 6 4 229 9 0.0 6 9 230 4 0.0 6 4 231 4 0.0 6 4 232 5 0.0 6 5 233 4 0.0 6 4 234 7 0.0 6 7 235 5 0.0 6 5 236 6 0.0 6 6 237 7 0.0 6 7 238 5 0.0 6 5 239 5 0.0 6 5 240 6 0.0 6 6 241 4 0.0 6 4 242 4 0.0 6 4 243 3 0.0 6 3 244 4 0.0 6 4 245 3 0.0 6 3 246 4 0.0 6 4 247 6 0.0 6 6 248 6 0.0 6 6 249 6 0.0 6 6 250 5 0.0 6 5 251 3 0.0 6 3 252 1 0.0 6 1 253 1 0.0 6 1 254 4 0.0 6 4 255 4 0.0 6 4 256 4 0.0 6 4 257 2 0.0 6 2 258 1 0.0 6 1 259 3 0.0 6 3 260 3 0.0 6 3 261 3 0.0 6 3 265 1 0.0 6 1 266 1 0.0 6 1 267 2 0.0 6 2 269 2 0.0 6 2 270 2 0.0 6 2 273 4 0.0 6 4 276 1 0.0 6 1 277 1 0.0 6 1 278 1 0.0 6 1 279 3 0.0 6 3 281 1 0.0 6 1 282 1 0.0 6 1 283 1 0.0 6 1 284 3 0.0 6 3 285 1 0.0 6 1 286 3 0.0 6 3 288 1 0.0 6 1 290 1 0.0 6 1 293 1 0.0 6 1 296 1 0.0 6 1 300 1 0.0 6 1 311 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 66867 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 16.7 1 1 9 2 0.3 2 2 10 1 0.1 3 1 11 2 0.0 3 2 12 1 0.0 3 1 13 11 0.0 3 9 2 14 7 0.0 4 5 2 15 2 0.0 4 2 16 14 0.0 4 14 17 21 0.0 5 20 1 18 5 0.0 5 5 19 17 0.0 5 14 2 1 20 116 0.0 6 114 2 21 62 0.0 6 61 1 22 39 0.0 6 38 0 0 1 23 34 0.0 6 34 24 11 0.0 6 11 25 23 0.0 6 23 26 21 0.0 6 20 0 0 0 1 27 27 0.0 6 25 2 28 13 0.0 6 12 1 29 16 0.0 6 11 3 0 1 1 30 16 0.0 6 14 2 31 16 0.0 6 16 32 11 0.0 6 10 1 33 15 0.0 6 14 0 1 34 15 0.0 6 14 1 35 10 0.0 6 10 36 15 0.0 6 15 37 19 0.0 6 15 3 0 1 38 12 0.0 6 12 39 9 0.0 6 8 1 40 13 0.0 6 12 1 41 10 0.0 6 8 2 42 13 0.0 6 13 43 11 0.0 6 10 1 44 17 0.0 6 15 1 0 1 45 15 0.0 6 15 46 16 0.0 6 14 2 47 22 0.0 6 19 3 48 17 0.0 6 14 2 0 0 0 0 1 49 15 0.0 6 15 50 16 0.0 6 15 1 51 22 0.0 6 18 4 52 17 0.0 6 17 53 25 0.0 6 24 0 0 1 54 21 0.0 6 20 1 55 14 0.0 6 14 56 19 0.0 6 15 2 0 1 1 57 16 0.0 6 15 0 0 1 58 24 0.0 6 23 0 1 59 24 0.0 6 22 2 60 20 0.0 6 18 1 0 0 1 61 33 0.0 6 30 1 0 1 1 62 28 0.0 6 26 2 63 27 0.0 6 25 2 64 34 0.0 6 33 1 65 37 0.0 6 34 3 66 42 0.0 6 40 1 0 0 0 0 1 67 29 0.0 6 27 2 68 44 0.0 6 39 3 1 1 69 153 0.0 6 149 4 70 55 0.0 6 53 2 71 60 0.0 6 58 2 72 41 0.0 6 37 3 0 0 1 73 56 0.0 6 55 1 74 52 0.0 6 47 5 75 43 0.0 6 41 2 76 50 0.0 6 47 3 77 48 0.0 6 46 1 1 78 36 0.0 6 35 1 79 33 0.0 6 30 3 80 59 0.0 6 57 2 81 48 0.0 6 46 2 82 53 0.0 6 51 2 83 63 0.0 6 61 2 84 51 0.0 6 50 1 85 84 0.0 6 78 3 1 1 1 86 47 0.0 6 46 1 87 39 0.0 6 39 88 50 0.0 6 47 2 0 1 89 43 0.0 6 41 2 90 53 0.0 6 50 3 91 61 0.0 6 58 3 92 74 0.0 6 73 1 93 65 0.0 6 63 1 1 94 65 0.0 6 64 0 0 0 1 95 84 0.0 6 81 2 0 1 96 59 0.0 6 56 2 0 0 0 1 97 80 0.0 6 80 98 103 0.0 6 102 1 99 94 0.0 6 89 5 100 93 0.0 6 92 1 101 83 0.0 6 80 2 0 0 0 0 1 102 70 0.0 6 68 1 0 0 0 1 103 95 0.0 6 92 2 0 0 1 104 126 0.0 6 123 3 105 124 0.0 6 119 5 106 183 0.0 6 177 6 107 116 0.0 6 115 1 108 101 0.0 6 97 4 109 112 0.0 6 106 6 110 111 0.0 6 106 5 111 118 0.0 6 114 4 112 143 0.0 6 141 2 113 156 0.0 6 151 4 0 0 0 0 1 114 161 0.0 6 159 2 115 142 0.0 6 140 2 116 139 0.0 6 131 7 1 117 152 0.0 6 151 1 118 175 0.0 6 170 3 0 1 1 119 173 0.0 6 166 7 120 206 0.0 6 202 4 121 177 0.0 6 173 3 1 122 186 0.0 6 182 3 0 1 123 205 0.0 6 205 124 191 0.0 6 187 2 0 0 1 0 1 125 224 0.0 6 218 4 0 0 0 0 2 126 239 0.0 6 237 2 127 281 0.0 6 276 4 1 128 283 0.0 6 281 1 1 129 311 0.0 6 303 7 1 130 368 0.0 6 352 15 1 131 485 0.0 6 474 9 0 0 0 1 1 132 364 0.0 6 358 6 133 397 0.0 6 390 6 0 0 0 0 1 134 385 0.0 6 377 7 0 0 1 135 457 0.0 6 443 13 0 1 136 478 0.0 6 465 11 0 1 0 1 137 463 0.0 6 456 3 1 1 1 0 1 138 480 0.0 6 468 6 0 0 1 3 2 139 442 0.0 6 435 5 0 1 0 0 1 140 636 0.0 6 619 13 1 3 141 759 0.0 6 754 3 1 0 0 1 142 953 0.0 6 946 6 0 1 143 915 0.0 6 904 10 0 1 144 1034 0.0 6 1024 7 1 1 0 1 145 1000 0.0 6 987 11 1 0 1 146 1100 0.0 6 1085 13 0 0 1 1 147 1150 0.0 6 1135 13 1 0 0 0 1 148 1117 0.0 6 1104 10 1 0 0 1 1 149 1221 0.0 6 1202 16 0 2 1 150 1616 0.0 6 1597 16 1 1 0 0 1 151 1274 0.0 6 1256 12 2 1 0 2 1 152 1416 0.0 6 1396 15 2 0 1 1 1 153 1174 0.0 6 1145 25 0 0 0 2 2 154 1322 0.0 6 1304 15 1 0 0 2 155 1279 0.0 6 1261 16 0 1 1 156 984 0.0 6 975 5 1 0 0 1 2 157 1121 0.0 6 1102 17 0 1 0 0 1 158 1040 0.0 6 1026 8 2 1 0 0 3 159 1117 0.0 6 1103 13 0 1 160 1109 0.0 6 1091 16 0 1 0 1 161 999 0.0 6 989 10 162 1000 0.0 6 987 10 0 0 0 1 2 163 897 0.0 6 885 10 1 0 0 0 1 164 762 0.0 6 754 7 0 1 165 836 0.0 6 826 7 0 0 0 0 3 166 829 0.0 6 821 7 0 0 0 1 167 713 0.0 6 703 6 1 2 0 0 1 168 970 0.0 6 954 13 1 0 2 169 801 0.0 6 793 5 2 0 0 0 1 170 878 0.0 6 867 9 1 0 0 1 171 823 0.0 6 811 10 1 0 0 0 1 172 746 0.0 6 736 6 1 0 1 1 1 173 878 0.0 6 872 5 0 1 174 816 0.0 6 807 8 1 175 838 0.0 6 822 12 2 0 0 1 1 176 779 0.0 6 760 11 3 4 1 177 766 0.0 6 754 9 1 0 0 2 178 793 0.0 6 782 9 0 1 0 1 179 670 0.0 6 660 7 2 0 1 180 639 0.0 6 633 5 1 181 553 0.0 6 550 3 182 447 0.0 6 443 3 0 0 0 1 183 502 0.0 6 496 6 184 407 0.0 6 405 2 185 637 0.0 6 631 5 0 0 0 1 186 833 0.0 6 815 13 3 0 0 1 1 187 704 0.0 6 695 9 188 657 0.0 6 651 5 1 189 535 0.0 6 523 10 1 0 0 1 190 554 0.0 6 542 12 191 528 0.0 6 519 6 1 0 1 1 192 389 0.0 6 382 7 193 428 0.0 6 420 8 194 393 0.0 6 391 2 195 447 0.0 6 437 9 1 196 540 0.0 6 530 10 197 244 0.0 6 241 3 198 505 0.0 6 496 7 0 0 1 1 199 551 0.0 6 536 15 200 325 0.0 6 317 6 1 0 0 0 1 201 353 0.0 6 348 4 0 1 202 248 0.0 6 243 5 203 232 0.0 6 226 6 204 275 0.0 6 271 3 0 0 0 0 1 205 158 0.0 6 158 206 151 0.0 6 148 3 207 139 0.0 6 138 1 208 152 0.0 6 150 2 209 137 0.0 6 135 2 210 128 0.0 6 125 3 211 151 0.0 6 151 212 129 0.0 6 127 2 213 87 0.0 6 86 1 214 124 0.0 6 122 2 215 138 0.0 6 136 2 216 164 0.0 6 160 4 217 136 0.0 6 133 3 218 113 0.0 6 109 4 219 140 0.0 6 138 2 220 84 0.0 6 81 2 0 0 1 221 94 0.0 6 92 2 222 46 0.0 6 45 1 223 97 0.0 6 97 224 109 0.0 6 105 4 225 125 0.0 6 123 2 226 101 0.0 6 100 1 227 64 0.0 6 62 2 228 33 0.0 6 31 2 229 67 0.0 6 66 1 230 49 0.0 6 45 4 231 41 0.0 6 41 232 50 0.0 6 49 1 233 71 0.0 6 65 5 1 234 49 0.0 6 49 235 21 0.0 6 21 236 12 0.0 6 12 237 16 0.0 6 16 238 9 0.0 6 9 239 5 0.0 6 5 240 14 0.0 6 14 241 16 0.0 6 16 242 32 0.0 6 32 243 31 0.0 6 31 244 20 0.0 6 19 1 245 10 0.0 6 10 246 10 0.0 6 10 247 8 0.0 6 8 248 14 0.0 6 14 249 6 0.0 6 6 250 5 0.0 6 4 1 251 2 0.0 6 2 252 2 0.0 6 2 253 5 0.0 6 5 255 1 0.0 6 1 256 1 0.0 6 1 278 1 0.0 6 1 279 3 0.0 6 3 280 12 0.0 6 12 281 104 0.0 6 102 1 0 0 1 282 1 0.0 6 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1306 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 47 1 0.0 6 0 1 51 1 0.0 6 1 54 1 0.0 6 1 57 1 0.0 6 1 58 8 0.0 6 7 1 61 2 0.0 6 2 62 1 0.0 6 1 63 5 0.0 6 5 64 1 0.0 6 1 65 2 0.0 6 2 66 1 0.0 6 1 67 1 0.0 6 1 68 3 0.0 6 3 69 14 0.0 6 13 1 70 22 0.0 6 22 71 18 0.0 6 18 72 11 0.0 6 10 1 73 12 0.0 6 12 74 17 0.0 6 17 75 30 0.0 6 30 76 34 0.0 6 34 77 31 0.0 6 31 78 24 0.0 6 24 79 17 0.0 6 17 80 37 0.0 6 37 81 7 0.0 6 7 82 12 0.0 6 11 0 0 0 1 83 12 0.0 6 12 84 10 0.0 6 10 85 9 0.0 6 9 86 6 0.0 6 6 87 12 0.0 6 12 88 11 0.0 6 11 89 14 0.0 6 14 90 17 0.0 6 17 91 10 0.0 6 9 1 92 9 0.0 6 9 93 7 0.0 6 7 94 7 0.0 6 7 95 9 0.0 6 9 96 10 0.0 6 10 97 5 0.0 6 5 98 9 0.0 6 9 99 12 0.0 6 12 100 17 0.0 6 17 101 14 0.0 6 14 102 12 0.0 6 12 103 13 0.0 6 13 104 5 0.0 6 5 105 10 0.0 6 10 106 12 0.0 6 10 2 107 10 0.0 6 10 108 40 0.0 6 40 109 11 0.0 6 11 110 11 0.0 6 11 111 11 0.0 6 11 112 10 0.0 6 10 113 5 0.0 6 5 114 5 0.0 6 5 115 3 0.0 6 3 116 8 0.0 6 8 117 7 0.0 6 7 118 7 0.0 6 7 119 6 0.0 6 6 120 10 0.0 6 9 1 121 3 0.0 6 3 122 3 0.0 6 3 123 13 0.0 6 13 124 8 0.0 6 8 125 9 0.0 6 9 126 15 0.0 6 15 127 9 0.0 6 9 128 1 0.0 6 1 129 6 0.0 6 6 130 4 0.0 6 4 131 14 0.0 6 14 132 15 0.0 6 15 133 6 0.0 6 6 134 9 0.0 6 9 135 13 0.0 6 12 0 0 1 136 10 0.0 6 10 137 6 0.0 6 6 138 7 0.0 6 7 139 5 0.0 6 5 140 11 0.0 6 11 141 7 0.0 6 7 142 9 0.0 6 9 143 6 0.0 6 6 144 4 0.0 6 4 145 6 0.0 6 6 146 7 0.0 6 7 147 10 0.0 6 10 148 6 0.0 6 6 149 9 0.0 6 9 150 4 0.0 6 4 151 6 0.0 6 6 152 7 0.0 6 7 153 11 0.0 6 11 154 3 0.0 6 3 155 7 0.0 6 7 156 12 0.0 6 12 157 11 0.0 6 10 0 0 0 1 158 3 0.0 6 3 159 2 0.0 6 2 160 7 0.0 6 7 161 9 0.0 6 9 162 9 0.0 6 9 163 5 0.0 6 5 164 4 0.0 6 4 165 5 0.0 6 5 166 7 0.0 6 7 167 10 0.0 6 10 168 5 0.0 6 5 169 17 0.0 6 17 170 6 0.0 6 6 171 5 0.0 6 4 0 0 0 1 172 3 0.0 6 3 173 6 0.0 6 6 174 5 0.0 6 5 175 16 0.0 6 16 176 5 0.0 6 5 177 4 0.0 6 4 178 4 0.0 6 4 179 5 0.0 6 5 180 10 0.0 6 10 181 7 0.0 6 7 182 5 0.0 6 5 183 10 0.0 6 10 184 6 0.0 6 6 185 4 0.0 6 4 186 5 0.0 6 5 187 5 0.0 6 4 1 188 4 0.0 6 4 189 13 0.0 6 13 190 6 0.0 6 6 191 5 0.0 6 4 0 1 192 4 0.0 6 4 193 3 0.0 6 3 194 4 0.0 6 4 195 7 0.0 6 7 196 4 0.0 6 4 197 2 0.0 6 2 198 2 0.0 6 2 199 1 0.0 6 1 200 4 0.0 6 4 201 1 0.0 6 1 202 4 0.0 6 4 203 1 0.0 6 1 204 2 0.0 6 2 205 2 0.0 6 2 206 2 0.0 6 2 207 3 0.0 6 3 208 5 0.0 6 5 209 3 0.0 6 3 211 1 0.0 6 1 212 1 0.0 6 1 213 1 0.0 6 1 215 1 0.0 6 1 216 5 0.0 6 5 217 4 0.0 6 4 218 1 0.0 6 1 219 1 0.0 6 1 220 1 0.0 6 1 221 1 0.0 6 1 222 1 0.0 6 1 223 1 0.0 6 1 224 1 0.0 6 1 230 1 0.0 6 1 231 1 0.0 6 1 232 1 0.0 6 1 237 1 0.0 6 1 239 1 0.0 6 1 242 1 0.0 6 1 250 1 0.0 6 1 257 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_06_17_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_06_244_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_06_17_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_06_244_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.06 s (31 µs/read; 1.92 M reads/minute). === Summary === Total read pairs processed: 66,025 Read 1 with adapter: 66,019 (100.0%) Read 2 with adapter: 65,994 (100.0%) == Read fate breakdown == Pairs that were too short: 25 (0.0%) Pairs discarded as untrimmed: 37 (0.1%) Pairs written (passing filters): 65,963 (99.9%) Total basepairs processed: 29,134,184 bp Read 1: 16,498,016 bp Read 2: 12,636,168 bp Total written (filtered): 4,091,554 bp (14.0%) Read 1: 2,155,426 bp Read 2: 1,936,128 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 64969 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1031.6 0 0 1 4 1 257.9 1 1 6 2 16.1 1 0 0 2 7 3 4.0 2 1 2 8 1 1.0 2 1 9 2 0.3 2 1 1 16 1 0.0 4 0 1 17 5 0.0 5 5 18 2 0.0 5 2 19 5 0.0 5 5 20 54 0.0 6 53 1 21 33 0.0 6 33 22 30 0.0 6 28 2 23 25 0.0 6 24 1 24 16 0.0 6 15 1 25 12 0.0 6 10 2 26 10 0.0 6 8 2 27 12 0.0 6 9 3 28 6 0.0 6 6 29 8 0.0 6 7 0 0 0 0 1 30 5 0.0 6 5 31 8 0.0 6 8 32 5 0.0 6 5 33 4 0.0 6 4 34 4 0.0 6 2 0 0 0 0 1 1 35 4 0.0 6 3 1 36 5 0.0 6 5 37 5 0.0 6 5 38 1 0.0 6 1 39 5 0.0 6 5 40 4 0.0 6 4 41 10 0.0 6 10 42 6 0.0 6 6 43 6 0.0 6 6 44 6 0.0 6 6 45 6 0.0 6 5 1 46 6 0.0 6 6 47 8 0.0 6 8 48 4 0.0 6 4 49 7 0.0 6 7 50 6 0.0 6 6 51 3 0.0 6 3 52 8 0.0 6 8 53 2 0.0 6 1 1 54 1 0.0 6 0 1 55 2 0.0 6 2 56 3 0.0 6 3 57 3 0.0 6 2 0 0 1 58 4 0.0 6 4 59 2 0.0 6 2 60 3 0.0 6 3 61 6 0.0 6 6 62 2 0.0 6 2 63 7 0.0 6 7 64 7 0.0 6 7 65 6 0.0 6 5 0 1 66 9 0.0 6 9 67 6 0.0 6 5 1 68 2 0.0 6 2 69 6 0.0 6 6 70 7 0.0 6 5 2 71 8 0.0 6 8 72 3 0.0 6 3 73 8 0.0 6 6 1 1 74 9 0.0 6 9 75 7 0.0 6 7 76 5 0.0 6 4 1 77 7 0.0 6 7 78 10 0.0 6 10 79 2 0.0 6 2 80 10 0.0 6 10 81 12 0.0 6 11 1 82 7 0.0 6 7 83 13 0.0 6 12 1 84 9 0.0 6 8 1 85 6 0.0 6 6 86 6 0.0 6 5 1 87 9 0.0 6 9 88 7 0.0 6 7 89 10 0.0 6 10 90 10 0.0 6 10 91 6 0.0 6 6 92 12 0.0 6 12 93 7 0.0 6 6 1 94 5 0.0 6 5 95 4 0.0 6 4 96 6 0.0 6 6 97 10 0.0 6 10 98 10 0.0 6 9 1 99 11 0.0 6 11 100 18 0.0 6 18 101 11 0.0 6 11 102 7 0.0 6 7 103 20 0.0 6 20 104 15 0.0 6 15 105 15 0.0 6 15 106 14 0.0 6 12 1 0 1 107 12 0.0 6 11 1 108 12 0.0 6 12 109 12 0.0 6 12 110 7 0.0 6 7 111 19 0.0 6 17 2 112 16 0.0 6 16 113 11 0.0 6 11 114 20 0.0 6 19 1 115 19 0.0 6 19 116 16 0.0 6 15 0 1 117 18 0.0 6 16 1 0 0 0 1 118 15 0.0 6 14 1 119 20 0.0 6 20 120 14 0.0 6 14 121 19 0.0 6 19 122 17 0.0 6 17 123 27 0.0 6 26 1 124 25 0.0 6 23 2 125 22 0.0 6 22 126 20 0.0 6 19 1 127 20 0.0 6 20 128 25 0.0 6 25 129 24 0.0 6 24 130 32 0.0 6 30 2 131 21 0.0 6 21 132 30 0.0 6 30 133 22 0.0 6 22 134 27 0.0 6 27 135 40 0.0 6 38 1 0 0 0 0 1 136 30 0.0 6 29 1 137 37 0.0 6 37 138 37 0.0 6 35 2 139 57 0.0 6 57 140 156 0.0 6 155 1 141 258 0.0 6 258 142 385 0.0 6 385 143 261 0.0 6 260 0 0 1 144 291 0.0 6 291 145 351 0.0 6 351 146 309 0.0 6 307 2 147 336 0.0 6 336 148 321 0.0 6 319 2 149 345 0.0 6 344 1 150 350 0.0 6 350 151 303 0.0 6 302 1 152 235 0.0 6 233 2 153 188 0.0 6 188 154 223 0.0 6 222 1 155 197 0.0 6 196 1 156 214 0.0 6 214 157 223 0.0 6 223 158 226 0.0 6 226 159 225 0.0 6 224 1 160 237 0.0 6 234 1 1 0 1 161 245 0.0 6 243 2 162 249 0.0 6 249 163 207 0.0 6 206 1 164 195 0.0 6 194 1 165 216 0.0 6 215 1 166 207 0.0 6 207 167 224 0.0 6 224 168 201 0.0 6 201 169 226 0.0 6 222 4 170 213 0.0 6 209 4 171 244 0.0 6 243 1 172 279 0.0 6 277 2 173 246 0.0 6 245 1 174 232 0.0 6 230 1 1 175 251 0.0 6 251 176 246 0.0 6 246 177 243 0.0 6 239 4 178 465 0.0 6 461 4 179 286 0.0 6 285 1 180 230 0.0 6 225 5 181 269 0.0 6 267 2 182 261 0.0 6 261 183 276 0.0 6 274 2 184 289 0.0 6 287 2 185 304 0.0 6 302 2 186 272 0.0 6 269 3 187 305 0.0 6 302 3 188 250 0.0 6 248 2 189 277 0.0 6 276 1 190 323 0.0 6 320 3 191 344 0.0 6 341 3 192 402 0.0 6 397 5 193 458 0.0 6 456 2 194 407 0.0 6 404 2 0 0 0 0 1 195 510 0.0 6 505 4 0 0 0 0 1 196 558 0.0 6 552 6 197 615 0.0 6 609 6 198 674 0.0 6 668 5 0 0 0 1 199 574 0.0 6 570 3 1 200 497 0.0 6 491 6 201 497 0.0 6 492 5 202 595 0.0 6 589 6 203 499 0.0 6 492 5 1 0 1 204 579 0.0 6 574 3 2 205 569 0.0 6 567 2 206 732 0.0 6 726 6 207 593 0.0 6 592 1 208 637 0.0 6 633 3 0 0 0 1 209 500 0.0 6 495 5 210 518 0.0 6 509 7 1 0 0 0 1 211 628 0.0 6 621 6 0 0 0 1 212 666 0.0 6 658 7 0 1 213 688 0.0 6 682 5 0 0 0 0 1 214 822 0.0 6 817 5 215 906 0.0 6 897 9 216 630 0.0 6 626 3 0 1 217 721 0.0 6 716 4 0 1 218 740 0.0 6 731 6 1 0 0 2 219 702 0.0 6 697 5 220 584 0.0 6 579 5 221 553 0.0 6 551 0 2 222 593 0.0 6 589 4 223 632 0.0 6 627 4 0 1 224 578 0.0 6 573 5 225 770 0.0 6 764 6 226 697 0.0 6 691 3 1 1 0 1 227 639 0.0 6 637 2 228 534 0.0 6 531 3 229 549 0.0 6 543 5 0 0 0 0 1 230 552 0.0 6 550 1 0 1 231 577 0.0 6 571 6 232 583 0.0 6 579 4 233 576 0.0 6 570 6 234 530 0.0 6 528 2 235 482 0.0 6 474 6 0 0 1 0 1 236 525 0.0 6 521 4 237 551 0.0 6 545 6 238 616 0.0 6 607 9 239 498 0.0 6 494 4 240 542 0.0 6 533 8 0 0 0 1 241 538 0.0 6 536 2 242 588 0.0 6 580 6 1 0 0 0 1 243 469 0.0 6 466 3 244 487 0.0 6 480 6 0 0 0 0 1 245 410 0.0 6 406 2 2 246 405 0.0 6 397 8 247 347 0.0 6 345 2 248 405 0.0 6 399 6 249 382 0.0 6 377 5 250 377 0.0 6 373 4 251 400 0.0 6 396 4 252 349 0.0 6 348 1 253 347 0.0 6 342 5 254 367 0.0 6 366 1 255 328 0.0 6 327 1 256 357 0.0 6 352 5 257 440 0.0 6 434 6 258 337 0.0 6 333 3 1 259 457 0.0 6 450 7 260 466 0.0 6 462 4 261 275 0.0 6 273 2 262 295 0.0 6 292 3 263 266 0.0 6 264 2 264 253 0.0 6 250 3 265 249 0.0 6 246 3 266 406 0.0 6 398 8 267 228 0.0 6 225 3 268 283 0.0 6 278 5 269 545 0.0 6 538 7 270 254 0.0 6 247 6 1 271 326 0.0 6 322 3 1 272 236 0.0 6 231 5 273 347 0.0 6 344 3 274 705 0.0 6 691 12 2 275 384 0.0 6 379 5 276 534 0.0 6 523 10 1 277 784 0.0 6 775 9 278 3419 0.0 6 3362 56 1 279 2600 0.0 6 2555 41 2 1 0 0 1 280 22 0.0 6 16 5 1 281 7 0.0 6 6 1 282 1 0.0 6 1 284 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1050 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 1 0.0 6 0 1 63 43 0.0 6 41 2 64 1 0.0 6 1 71 2 0.0 6 2 72 7 0.0 6 7 73 8 0.0 6 8 74 12 0.0 6 12 75 4 0.0 6 4 76 14 0.0 6 14 77 5 0.0 6 5 78 7 0.0 6 7 79 10 0.0 6 10 80 12 0.0 6 12 81 9 0.0 6 9 82 5 0.0 6 5 83 5 0.0 6 5 84 5 0.0 6 5 85 9 0.0 6 9 86 7 0.0 6 7 87 3 0.0 6 3 88 3 0.0 6 3 89 4 0.0 6 4 90 5 0.0 6 5 91 6 0.0 6 6 92 7 0.0 6 7 93 6 0.0 6 6 94 3 0.0 6 3 95 3 0.0 6 3 96 5 0.0 6 5 97 3 0.0 6 3 98 4 0.0 6 4 99 3 0.0 6 3 100 2 0.0 6 2 101 4 0.0 6 4 102 8 0.0 6 8 103 4 0.0 6 4 104 2 0.0 6 2 105 2 0.0 6 2 106 3 0.0 6 3 107 2 0.0 6 2 108 3 0.0 6 3 109 7 0.0 6 7 110 5 0.0 6 5 111 2 0.0 6 2 112 3 0.0 6 3 113 7 0.0 6 7 114 4 0.0 6 4 115 3 0.0 6 3 116 2 0.0 6 2 117 2 0.0 6 2 118 5 0.0 6 5 119 3 0.0 6 3 120 4 0.0 6 4 122 6 0.0 6 6 123 1 0.0 6 1 124 1 0.0 6 1 125 6 0.0 6 6 126 2 0.0 6 2 127 5 0.0 6 5 128 7 0.0 6 7 129 6 0.0 6 6 130 5 0.0 6 5 131 5 0.0 6 5 132 9 0.0 6 9 133 5 0.0 6 5 134 7 0.0 6 7 135 3 0.0 6 3 136 5 0.0 6 5 137 3 0.0 6 3 138 6 0.0 6 6 139 7 0.0 6 7 140 2 0.0 6 2 141 7 0.0 6 7 142 4 0.0 6 4 143 3 0.0 6 3 144 4 0.0 6 4 145 4 0.0 6 4 146 5 0.0 6 5 147 4 0.0 6 4 148 7 0.0 6 7 149 3 0.0 6 3 150 5 0.0 6 5 151 7 0.0 6 7 152 6 0.0 6 6 153 6 0.0 6 6 154 9 0.0 6 9 155 8 0.0 6 8 156 5 0.0 6 5 157 6 0.0 6 6 158 8 0.0 6 8 159 1 0.0 6 1 160 9 0.0 6 9 161 8 0.0 6 8 162 4 0.0 6 4 163 11 0.0 6 11 164 5 0.0 6 5 165 6 0.0 6 5 1 166 8 0.0 6 8 167 5 0.0 6 5 168 4 0.0 6 4 169 5 0.0 6 5 170 4 0.0 6 4 171 7 0.0 6 7 172 11 0.0 6 11 173 3 0.0 6 3 174 7 0.0 6 7 175 5 0.0 6 5 176 6 0.0 6 6 177 8 0.0 6 8 178 3 0.0 6 3 179 5 0.0 6 5 180 1 0.0 6 1 181 1 0.0 6 1 182 9 0.0 6 9 183 5 0.0 6 4 1 184 7 0.0 6 7 185 6 0.0 6 6 186 4 0.0 6 4 187 11 0.0 6 11 188 4 0.0 6 4 189 5 0.0 6 5 190 3 0.0 6 3 191 5 0.0 6 5 192 5 0.0 6 5 193 3 0.0 6 3 194 12 0.0 6 12 195 5 0.0 6 5 196 2 0.0 6 2 197 4 0.0 6 4 198 4 0.0 6 4 199 11 0.0 6 11 200 9 0.0 6 9 201 5 0.0 6 5 202 5 0.0 6 5 203 8 0.0 6 8 204 8 0.0 6 8 205 3 0.0 6 3 206 6 0.0 6 6 207 9 0.0 6 9 208 3 0.0 6 3 209 8 0.0 6 8 210 3 0.0 6 3 211 7 0.0 6 6 1 212 7 0.0 6 7 213 7 0.0 6 7 214 7 0.0 6 6 1 215 7 0.0 6 7 216 7 0.0 6 7 217 3 0.0 6 3 218 5 0.0 6 5 220 10 0.0 6 10 221 5 0.0 6 5 222 6 0.0 6 6 223 5 0.0 6 5 224 5 0.0 6 5 225 6 0.0 6 6 226 7 0.0 6 7 227 8 0.0 6 8 228 5 0.0 6 5 229 7 0.0 6 7 230 7 0.0 6 7 231 6 0.0 6 6 232 3 0.0 6 3 233 2 0.0 6 2 234 1 0.0 6 1 235 6 0.0 6 6 236 9 0.0 6 9 237 2 0.0 6 2 238 4 0.0 6 4 239 6 0.0 6 6 240 2 0.0 6 2 241 6 0.0 6 6 242 1 0.0 6 1 243 1 0.0 6 1 244 3 0.0 6 3 245 6 0.0 6 6 246 5 0.0 6 5 247 2 0.0 6 2 248 3 0.0 6 3 249 4 0.0 6 3 1 250 3 0.0 6 3 251 4 0.0 6 4 252 2 0.0 6 2 253 2 0.0 6 2 254 4 0.0 6 4 255 4 0.0 6 4 256 3 0.0 6 3 257 3 0.0 6 3 258 3 0.0 6 3 259 1 0.0 6 1 260 2 0.0 6 2 261 1 0.0 6 1 262 2 0.0 6 2 263 2 0.0 6 2 264 3 0.0 6 3 266 2 0.0 6 2 268 2 0.0 6 2 269 1 0.0 6 1 270 2 0.0 6 1 1 272 1 0.0 6 1 273 2 0.0 6 2 274 1 0.0 6 1 276 1 0.0 6 1 278 1 0.0 6 1 279 2 0.0 6 2 280 4 0.0 6 4 282 1 0.0 6 1 284 3 0.0 6 3 288 1 0.0 6 1 290 2 0.0 6 2 294 1 0.0 6 0 1 297 1 0.0 6 1 299 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 65192 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 257.9 1 1 6 1 16.1 1 1 7 1 4.0 2 1 9 1 0.3 2 1 11 5 0.0 3 3 2 12 7 0.0 3 6 1 13 5 0.0 3 5 14 7 0.0 4 6 1 15 4 0.0 4 4 16 8 0.0 4 8 17 21 0.0 5 19 2 18 3 0.0 5 2 1 19 53 0.0 5 48 5 20 92 0.0 6 90 2 21 64 0.0 6 60 4 22 44 0.0 6 40 4 23 19 0.0 6 19 24 26 0.0 6 25 0 0 1 25 17 0.0 6 16 0 1 26 13 0.0 6 13 27 15 0.0 6 15 28 13 0.0 6 13 29 29 0.0 6 28 1 30 6 0.0 6 6 31 16 0.0 6 13 3 32 14 0.0 6 11 3 33 9 0.0 6 8 1 34 21 0.0 6 20 1 35 6 0.0 6 5 1 36 14 0.0 6 13 0 0 0 0 0 1 37 18 0.0 6 17 1 38 14 0.0 6 14 39 12 0.0 6 12 40 13 0.0 6 11 1 0 1 41 20 0.0 6 19 1 42 15 0.0 6 11 3 1 43 11 0.0 6 10 1 44 11 0.0 6 9 2 45 22 0.0 6 22 46 16 0.0 6 14 2 47 13 0.0 6 11 1 1 48 17 0.0 6 16 1 49 17 0.0 6 16 1 50 25 0.0 6 23 2 51 22 0.0 6 20 1 0 0 1 52 23 0.0 6 22 1 53 16 0.0 6 14 1 0 1 54 22 0.0 6 19 2 1 55 17 0.0 6 15 1 0 1 56 32 0.0 6 28 4 57 18 0.0 6 12 3 2 1 58 24 0.0 6 22 2 59 19 0.0 6 16 3 60 20 0.0 6 19 1 61 18 0.0 6 17 1 62 27 0.0 6 26 1 63 26 0.0 6 26 64 17 0.0 6 17 65 23 0.0 6 21 1 0 0 0 1 66 38 0.0 6 38 67 29 0.0 6 28 1 68 36 0.0 6 34 2 69 45 0.0 6 42 3 70 120 0.0 6 116 3 1 71 54 0.0 6 54 72 69 0.0 6 67 1 1 73 35 0.0 6 32 2 1 74 53 0.0 6 50 2 0 0 1 75 42 0.0 6 41 1 76 21 0.0 6 21 77 33 0.0 6 33 78 47 0.0 6 45 1 1 79 45 0.0 6 44 1 80 34 0.0 6 32 2 81 42 0.0 6 38 2 2 82 59 0.0 6 55 2 1 1 83 49 0.0 6 43 4 0 1 0 0 1 84 61 0.0 6 56 4 0 0 0 1 85 51 0.0 6 51 86 80 0.0 6 78 1 1 87 67 0.0 6 66 1 88 53 0.0 6 50 2 1 89 45 0.0 6 42 3 90 64 0.0 6 62 2 91 62 0.0 6 55 4 2 0 0 1 92 51 0.0 6 48 3 93 70 0.0 6 67 3 94 76 0.0 6 73 0 2 1 95 60 0.0 6 59 1 96 96 0.0 6 90 4 1 0 1 97 68 0.0 6 65 2 1 98 70 0.0 6 69 1 99 90 0.0 6 86 3 0 0 1 100 87 0.0 6 85 2 101 75 0.0 6 73 2 102 85 0.0 6 82 0 0 2 1 103 91 0.0 6 85 5 0 0 1 104 89 0.0 6 88 1 105 92 0.0 6 88 4 106 102 0.0 6 98 3 0 0 0 0 1 107 181 0.0 6 174 7 108 116 0.0 6 113 2 0 0 0 1 109 101 0.0 6 96 5 110 93 0.0 6 92 1 111 83 0.0 6 80 3 112 113 0.0 6 111 2 113 114 0.0 6 108 3 2 1 114 129 0.0 6 124 5 115 161 0.0 6 156 4 0 0 1 116 128 0.0 6 122 5 1 117 128 0.0 6 126 2 118 166 0.0 6 160 5 1 119 151 0.0 6 147 4 120 182 0.0 6 175 6 0 0 0 0 1 121 165 0.0 6 157 5 1 2 122 155 0.0 6 150 5 123 190 0.0 6 188 2 124 191 0.0 6 184 6 1 125 174 0.0 6 171 1 1 0 0 0 1 126 193 0.0 6 188 3 1 0 1 127 254 0.0 6 247 7 128 249 0.0 6 238 10 1 129 244 0.0 6 239 4 0 0 0 0 1 130 272 0.0 6 264 8 131 318 0.0 6 311 7 132 295 0.0 6 292 2 0 1 133 310 0.0 6 300 8 0 1 0 0 1 134 370 0.0 6 358 11 1 135 388 0.0 6 374 11 1 1 0 1 136 401 0.0 6 394 2 0 1 4 137 433 0.0 6 417 14 0 0 0 1 1 138 469 0.0 6 462 6 1 139 396 0.0 6 387 9 140 524 0.0 6 513 9 1 1 141 606 0.0 6 600 3 1 1 0 0 1 142 739 0.0 6 724 12 0 1 0 0 2 143 668 0.0 6 659 6 1 0 0 0 2 144 780 0.0 6 766 13 1 145 831 0.0 6 823 7 0 0 0 1 146 739 0.0 6 729 9 1 147 824 0.0 6 818 6 148 860 0.0 6 848 9 1 0 0 2 149 880 0.0 6 868 10 0 0 0 0 2 150 933 0.0 6 920 12 0 0 1 151 1041 0.0 6 1025 13 1 1 0 1 152 1098 0.0 6 1076 20 0 0 1 0 1 153 1323 0.0 6 1300 20 1 0 0 1 1 154 1026 0.0 6 1011 14 0 0 0 1 155 1221 0.0 6 1207 12 0 0 2 156 1236 0.0 6 1218 11 3 4 157 1055 0.0 6 1043 11 0 0 1 158 1086 0.0 6 1070 13 1 0 0 2 159 957 0.0 6 940 15 1 0 0 0 1 160 1087 0.0 6 1074 12 0 0 0 1 161 1030 0.0 6 1020 8 1 0 0 0 1 162 917 0.0 6 903 11 1 0 1 0 1 163 930 0.0 6 916 11 2 0 1 164 798 0.0 6 787 6 1 0 2 0 2 165 766 0.0 6 758 6 0 1 0 0 1 166 903 0.0 6 894 9 167 715 0.0 6 706 7 0 1 1 168 802 0.0 6 787 15 169 880 0.0 6 868 8 1 1 0 0 2 170 660 0.0 6 653 7 171 947 0.0 6 929 15 1 0 1 1 172 764 0.0 6 756 7 0 0 1 173 736 0.0 6 730 6 174 808 0.0 6 794 12 0 1 0 0 1 175 995 0.0 6 984 10 0 0 0 0 1 176 849 0.0 6 836 12 0 1 177 745 0.0 6 735 8 1 0 0 1 178 773 0.0 6 757 13 2 0 1 179 764 0.0 6 753 8 1 1 0 1 180 622 0.0 6 611 10 0 0 0 1 181 727 0.0 6 714 12 0 0 1 182 532 0.0 6 528 4 183 594 0.0 6 585 6 1 2 184 410 0.0 6 406 4 185 391 0.0 6 382 8 0 0 1 186 734 0.0 6 720 13 0 1 187 860 0.0 6 849 10 0 0 1 188 844 0.0 6 829 13 1 0 1 189 597 0.0 6 590 6 1 190 751 0.0 6 738 12 0 0 0 1 191 629 0.0 6 619 9 0 0 0 0 1 192 523 0.0 6 516 6 1 193 496 0.0 6 491 2 0 2 1 194 594 0.0 6 587 6 1 195 510 0.0 6 497 13 196 852 0.0 6 839 12 1 197 331 0.0 6 327 4 198 484 0.0 6 474 10 199 931 0.0 6 913 17 0 0 1 200 405 0.0 6 397 8 201 470 0.0 6 461 8 1 202 335 0.0 6 329 6 203 308 0.0 6 302 5 1 204 280 0.0 6 275 5 205 227 0.0 6 225 2 206 186 0.0 6 182 4 207 145 0.0 6 139 4 1 0 1 208 172 0.0 6 170 2 209 179 0.0 6 176 3 210 146 0.0 6 146 211 170 0.0 6 167 3 212 195 0.0 6 187 7 0 0 0 1 213 78 0.0 6 74 4 214 106 0.0 6 105 1 215 105 0.0 6 103 2 216 191 0.0 6 185 5 1 217 312 0.0 6 308 4 218 156 0.0 6 153 3 219 199 0.0 6 195 4 220 139 0.0 6 138 1 221 272 0.0 6 266 6 222 101 0.0 6 99 2 223 181 0.0 6 176 5 224 104 0.0 6 103 1 225 136 0.0 6 135 1 226 120 0.0 6 119 1 227 101 0.0 6 98 3 228 53 0.0 6 53 229 116 0.0 6 112 4 230 47 0.0 6 45 2 231 70 0.0 6 67 3 232 75 0.0 6 73 2 233 100 0.0 6 97 2 1 234 38 0.0 6 38 235 36 0.0 6 35 1 236 13 0.0 6 13 237 23 0.0 6 22 1 238 6 0.0 6 6 239 13 0.0 6 13 240 17 0.0 6 17 241 43 0.0 6 42 0 1 242 54 0.0 6 52 2 243 21 0.0 6 21 244 68 0.0 6 68 245 35 0.0 6 33 2 246 31 0.0 6 30 1 247 11 0.0 6 11 248 18 0.0 6 17 1 249 9 0.0 6 9 250 10 0.0 6 10 251 20 0.0 6 20 252 3 0.0 6 3 253 3 0.0 6 3 254 10 0.0 6 10 255 1 0.0 6 1 256 3 0.0 6 3 279 1 0.0 6 1 280 1 0.0 6 1 281 11 0.0 6 10 1 282 73 0.0 6 73 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 802 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 1 0.0 6 0 1 52 2 0.0 6 2 56 1 0.0 6 1 57 1 0.0 6 1 60 1 0.0 6 1 63 2 0.0 6 2 66 2 0.0 6 2 68 7 0.0 6 7 69 4 0.0 6 4 70 4 0.0 6 4 71 13 0.0 6 13 72 6 0.0 6 6 73 9 0.0 6 9 74 5 0.0 6 5 75 13 0.0 6 13 76 13 0.0 6 13 77 8 0.0 6 8 78 12 0.0 6 11 1 79 9 0.0 6 9 80 8 0.0 6 8 81 7 0.0 6 7 82 4 0.0 6 4 83 5 0.0 6 5 84 2 0.0 6 2 85 3 0.0 6 3 86 11 0.0 6 11 87 5 0.0 6 5 88 3 0.0 6 3 89 3 0.0 6 3 90 6 0.0 6 5 1 91 2 0.0 6 2 92 2 0.0 6 2 93 5 0.0 6 5 94 2 0.0 6 2 95 4 0.0 6 4 96 4 0.0 6 4 97 3 0.0 6 3 98 4 0.0 6 4 99 5 0.0 6 5 100 7 0.0 6 7 101 3 0.0 6 3 102 6 0.0 6 6 103 3 0.0 6 3 104 5 0.0 6 5 105 6 0.0 6 6 106 8 0.0 6 8 107 21 0.0 6 21 108 6 0.0 6 6 109 2 0.0 6 2 110 5 0.0 6 5 111 6 0.0 6 6 112 3 0.0 6 3 113 6 0.0 6 6 114 6 0.0 6 6 115 2 0.0 6 1 0 0 0 1 116 4 0.0 6 4 117 2 0.0 6 2 118 8 0.0 6 8 119 4 0.0 6 4 120 2 0.0 6 2 121 4 0.0 6 4 122 4 0.0 6 4 123 7 0.0 6 7 124 7 0.0 6 7 125 5 0.0 6 5 126 3 0.0 6 3 127 2 0.0 6 2 128 10 0.0 6 10 129 4 0.0 6 4 130 3 0.0 6 3 131 17 0.0 6 16 0 0 0 1 132 5 0.0 6 5 133 7 0.0 6 7 134 6 0.0 6 6 135 5 0.0 6 5 136 2 0.0 6 1 1 137 5 0.0 6 5 138 5 0.0 6 5 139 6 0.0 6 5 1 140 2 0.0 6 2 141 3 0.0 6 3 142 7 0.0 6 7 143 8 0.0 6 8 144 10 0.0 6 10 145 3 0.0 6 3 146 8 0.0 6 8 147 3 0.0 6 3 148 7 0.0 6 7 149 7 0.0 6 7 150 2 0.0 6 2 151 8 0.0 6 8 152 6 0.0 6 6 153 13 0.0 6 13 154 3 0.0 6 3 155 7 0.0 6 7 156 8 0.0 6 8 157 6 0.0 6 6 158 5 0.0 6 5 159 5 0.0 6 5 160 6 0.0 6 6 161 5 0.0 6 5 162 8 0.0 6 8 163 6 0.0 6 6 164 14 0.0 6 14 165 2 0.0 6 2 166 6 0.0 6 6 167 9 0.0 6 9 168 4 0.0 6 4 169 7 0.0 6 7 170 4 0.0 6 4 171 11 0.0 6 11 172 5 0.0 6 5 173 2 0.0 6 2 174 6 0.0 6 6 175 4 0.0 6 4 176 8 0.0 6 8 177 7 0.0 6 7 178 3 0.0 6 3 179 7 0.0 6 7 180 3 0.0 6 3 181 8 0.0 6 8 182 9 0.0 6 9 183 5 0.0 6 5 184 6 0.0 6 6 185 6 0.0 6 6 186 3 0.0 6 3 187 7 0.0 6 7 188 6 0.0 6 6 189 4 0.0 6 4 190 3 0.0 6 3 191 10 0.0 6 9 0 0 0 1 192 2 0.0 6 2 193 2 0.0 6 2 194 2 0.0 6 2 195 5 0.0 6 5 196 3 0.0 6 3 197 5 0.0 6 5 198 2 0.0 6 2 199 1 0.0 6 1 200 5 0.0 6 5 201 1 0.0 6 1 202 2 0.0 6 2 203 2 0.0 6 2 205 3 0.0 6 3 207 1 0.0 6 1 208 4 0.0 6 4 209 1 0.0 6 1 210 3 0.0 6 3 211 1 0.0 6 1 212 3 0.0 6 3 213 1 0.0 6 1 214 3 0.0 6 3 216 1 0.0 6 1 222 1 0.0 6 1 225 1 0.0 6 1 226 1 0.0 6 1 228 1 0.0 6 1 229 2 0.0 6 2 230 1 0.0 6 1 231 1 0.0 6 0 1 240 1 0.0 6 1 251 1 0.0 6 1 260 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_15_18_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_15_245_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_15_18_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_15_245_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.58 s (21 µs/read; 2.88 M reads/minute). === Summary === Total read pairs processed: 75,678 Read 1 with adapter: 75,667 (100.0%) Read 2 with adapter: 75,631 (99.9%) == Read fate breakdown == Pairs that were too short: 45 (0.1%) Pairs discarded as untrimmed: 57 (0.1%) Pairs written (passing filters): 75,576 (99.9%) Total basepairs processed: 32,650,517 bp Read 1: 18,138,276 bp Read 2: 14,512,241 bp Total written (filtered): 5,030,813 bp (15.4%) Read 1: 2,540,726 bp Read 2: 2,490,087 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 73298 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1182.5 0 0 1 4 6 295.6 1 4 2 6 2 18.5 1 1 0 1 9 2 0.3 2 1 1 11 2 0.0 3 2 13 1 0.0 3 1 17 3 0.0 5 2 1 18 2 0.0 5 1 0 1 20 96 0.0 6 89 7 21 72 0.0 6 69 3 22 37 0.0 6 35 2 23 25 0.0 6 25 24 23 0.0 6 22 1 25 19 0.0 6 19 26 11 0.0 6 11 27 13 0.0 6 12 1 28 11 0.0 6 9 1 0 0 1 29 9 0.0 6 9 30 9 0.0 6 6 3 31 6 0.0 6 6 32 10 0.0 6 10 33 4 0.0 6 4 34 8 0.0 6 8 35 5 0.0 6 5 36 5 0.0 6 5 37 9 0.0 6 8 1 38 5 0.0 6 4 1 39 5 0.0 6 5 40 9 0.0 6 8 1 41 9 0.0 6 9 42 5 0.0 6 5 43 7 0.0 6 7 44 6 0.0 6 5 1 45 6 0.0 6 6 46 6 0.0 6 6 47 5 0.0 6 5 48 9 0.0 6 8 1 49 8 0.0 6 8 50 3 0.0 6 2 1 51 5 0.0 6 5 52 6 0.0 6 6 53 8 0.0 6 8 54 6 0.0 6 6 55 4 0.0 6 4 56 2 0.0 6 2 57 5 0.0 6 4 0 0 0 0 0 1 58 5 0.0 6 5 59 1 0.0 6 1 60 2 0.0 6 2 61 6 0.0 6 6 62 3 0.0 6 3 63 5 0.0 6 5 64 5 0.0 6 4 0 1 65 10 0.0 6 10 66 7 0.0 6 7 67 5 0.0 6 4 1 68 11 0.0 6 10 1 69 6 0.0 6 5 1 70 11 0.0 6 11 71 9 0.0 6 9 72 6 0.0 6 4 1 1 73 8 0.0 6 7 0 0 0 0 0 1 74 10 0.0 6 9 1 75 10 0.0 6 9 1 76 9 0.0 6 8 1 77 7 0.0 6 6 1 78 5 0.0 6 5 79 8 0.0 6 7 0 0 0 0 0 1 80 11 0.0 6 11 81 7 0.0 6 7 82 8 0.0 6 6 1 0 0 1 83 13 0.0 6 13 84 7 0.0 6 7 85 8 0.0 6 8 86 9 0.0 6 8 0 1 87 12 0.0 6 12 88 7 0.0 6 7 89 11 0.0 6 11 90 16 0.0 6 16 91 6 0.0 6 5 1 92 7 0.0 6 7 93 10 0.0 6 8 1 0 0 0 0 1 94 6 0.0 6 6 95 12 0.0 6 10 2 96 11 0.0 6 11 97 5 0.0 6 5 98 9 0.0 6 9 99 6 0.0 6 6 100 14 0.0 6 13 1 101 10 0.0 6 10 102 19 0.0 6 19 103 17 0.0 6 16 1 104 13 0.0 6 12 1 105 19 0.0 6 17 0 1 1 106 16 0.0 6 15 1 107 11 0.0 6 11 108 14 0.0 6 13 1 109 13 0.0 6 12 1 110 21 0.0 6 19 1 0 0 0 0 1 111 7 0.0 6 7 112 20 0.0 6 19 1 113 18 0.0 6 17 1 114 18 0.0 6 18 115 19 0.0 6 17 2 116 21 0.0 6 20 1 117 24 0.0 6 24 118 17 0.0 6 16 1 119 19 0.0 6 18 1 120 18 0.0 6 17 1 121 9 0.0 6 9 122 19 0.0 6 19 123 19 0.0 6 18 0 0 0 1 124 29 0.0 6 27 2 125 26 0.0 6 26 126 101 0.0 6 98 1 0 0 0 0 2 127 32 0.0 6 32 128 24 0.0 6 24 129 28 0.0 6 26 1 0 1 130 37 0.0 6 36 1 131 23 0.0 6 23 132 23 0.0 6 23 133 29 0.0 6 29 134 35 0.0 6 34 1 135 32 0.0 6 32 136 31 0.0 6 30 1 137 37 0.0 6 36 1 138 32 0.0 6 32 139 31 0.0 6 31 140 60 0.0 6 60 141 44 0.0 6 43 1 142 75 0.0 6 74 1 143 190 0.0 6 190 144 265 0.0 6 263 2 145 314 0.0 6 314 146 381 0.0 6 379 2 147 297 0.0 6 296 1 148 439 0.0 6 437 2 149 443 0.0 6 441 2 150 361 0.0 6 359 2 151 271 0.0 6 270 1 152 306 0.0 6 301 5 153 440 0.0 6 439 0 0 0 0 1 154 212 0.0 6 212 155 309 0.0 6 307 2 156 360 0.0 6 359 1 157 274 0.0 6 272 2 158 329 0.0 6 326 3 159 221 0.0 6 218 2 0 1 160 240 0.0 6 239 0 1 161 268 0.0 6 264 3 1 162 464 0.0 6 458 3 1 2 163 475 0.0 6 472 3 164 468 0.0 6 465 3 165 316 0.0 6 316 166 464 0.0 6 463 1 167 339 0.0 6 337 1 0 1 168 332 0.0 6 331 1 169 476 0.0 6 474 2 170 267 0.0 6 263 4 171 318 0.0 6 318 172 406 0.0 6 406 173 395 0.0 6 395 174 305 0.0 6 305 175 490 0.0 6 486 3 1 176 492 0.0 6 492 177 323 0.0 6 320 2 0 0 0 0 1 178 442 0.0 6 441 1 179 454 0.0 6 450 4 180 324 0.0 6 321 3 181 762 0.0 6 760 0 0 0 0 2 182 483 0.0 6 481 1 0 1 183 448 0.0 6 443 5 184 423 0.0 6 421 2 185 437 0.0 6 433 4 186 411 0.0 6 408 2 0 1 187 531 0.0 6 529 1 0 1 188 445 0.0 6 443 0 1 1 189 394 0.0 6 388 6 190 531 0.0 6 529 2 191 596 0.0 6 594 2 192 478 0.0 6 474 4 193 568 0.0 6 565 3 194 642 0.0 6 638 3 0 0 0 0 1 195 821 0.0 6 818 3 196 983 0.0 6 979 4 197 1062 0.0 6 1057 5 198 971 0.0 6 968 2 0 0 1 199 919 0.0 6 916 3 200 698 0.0 6 694 2 1 1 201 800 0.0 6 799 1 202 846 0.0 6 845 1 203 728 0.0 6 722 6 204 677 0.0 6 673 4 205 931 0.0 6 928 3 206 958 0.0 6 946 10 1 0 0 0 1 207 947 0.0 6 943 4 208 999 0.0 6 992 6 0 1 209 672 0.0 6 669 3 210 723 0.0 6 720 3 211 1051 0.0 6 1049 1 0 0 0 0 1 212 1235 0.0 6 1227 7 0 0 1 213 941 0.0 6 934 5 1 0 0 1 214 1072 0.0 6 1067 5 215 1207 0.0 6 1201 5 1 216 934 0.0 6 928 5 0 1 217 935 0.0 6 931 4 218 991 0.0 6 982 7 2 219 837 0.0 6 829 8 220 765 0.0 6 763 2 221 696 0.0 6 695 1 222 699 0.0 6 695 4 223 847 0.0 6 840 7 224 915 0.0 6 912 2 0 1 225 927 0.0 6 919 7 0 0 0 0 1 226 955 0.0 6 951 4 227 844 0.0 6 840 4 228 722 0.0 6 716 6 229 774 0.0 6 771 3 230 815 0.0 6 807 8 231 692 0.0 6 687 5 232 681 0.0 6 674 7 233 701 0.0 6 697 3 1 234 619 0.0 6 610 7 0 1 0 0 1 235 542 0.0 6 541 1 236 575 0.0 6 571 3 1 237 581 0.0 6 580 1 238 549 0.0 6 546 2 0 1 239 539 0.0 6 534 4 0 0 0 0 1 240 519 0.0 6 515 3 0 0 0 0 1 241 555 0.0 6 554 1 242 479 0.0 6 478 1 243 509 0.0 6 508 1 244 442 0.0 6 441 0 0 1 245 367 0.0 6 364 3 246 386 0.0 6 382 3 1 247 428 0.0 6 426 1 0 0 0 0 1 248 366 0.0 6 365 0 0 0 1 249 327 0.0 6 326 1 250 325 0.0 6 323 2 251 339 0.0 6 338 1 252 293 0.0 6 292 0 0 0 0 1 253 294 0.0 6 290 3 0 0 0 0 1 254 254 0.0 6 253 0 0 0 1 255 243 0.0 6 238 5 256 250 0.0 6 248 2 257 268 0.0 6 265 2 1 258 257 0.0 6 255 2 259 267 0.0 6 266 1 260 236 0.0 6 233 3 261 167 0.0 6 165 2 262 205 0.0 6 201 4 263 178 0.0 6 177 1 264 207 0.0 6 206 1 265 164 0.0 6 163 1 266 156 0.0 6 155 1 267 173 0.0 6 172 1 268 169 0.0 6 168 1 269 234 0.0 6 232 2 270 192 0.0 6 187 3 1 0 1 271 159 0.0 6 158 1 272 143 0.0 6 141 2 273 201 0.0 6 200 1 274 248 0.0 6 247 1 275 230 0.0 6 227 3 276 294 0.0 6 291 3 277 320 0.0 6 319 1 278 1040 0.0 6 1025 14 0 0 0 1 279 1439 0.0 6 1425 11 1 0 1 1 280 9 0.0 6 9 281 2 0.0 6 2 282 1 0.0 6 1 283 1 0.0 6 1 287 1 0.0 6 1 289 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2369 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 1 0.0 6 0 1 63 96 0.0 6 92 4 69 1 0.0 6 1 71 6 0.0 6 6 72 11 0.0 6 11 73 7 0.0 6 7 74 9 0.0 6 9 75 10 0.0 6 10 76 9 0.0 6 9 77 12 0.0 6 12 78 17 0.0 6 17 79 12 0.0 6 12 80 12 0.0 6 12 81 10 0.0 6 10 82 8 0.0 6 8 83 10 0.0 6 10 84 8 0.0 6 8 85 6 0.0 6 6 86 13 0.0 6 13 87 10 0.0 6 10 88 11 0.0 6 11 89 10 0.0 6 10 90 8 0.0 6 8 91 5 0.0 6 5 92 9 0.0 6 9 93 20 0.0 6 20 94 10 0.0 6 9 1 95 13 0.0 6 13 96 3 0.0 6 3 97 10 0.0 6 10 98 14 0.0 6 14 99 9 0.0 6 9 100 11 0.0 6 11 101 5 0.0 6 5 102 9 0.0 6 9 103 5 0.0 6 5 104 21 0.0 6 21 105 10 0.0 6 10 106 14 0.0 6 14 107 10 0.0 6 10 108 13 0.0 6 13 109 19 0.0 6 19 110 22 0.0 6 22 111 10 0.0 6 10 112 15 0.0 6 15 113 12 0.0 6 12 114 3 0.0 6 3 115 6 0.0 6 6 116 11 0.0 6 11 117 14 0.0 6 14 118 12 0.0 6 12 119 14 0.0 6 14 120 12 0.0 6 12 121 9 0.0 6 9 122 10 0.0 6 10 123 18 0.0 6 18 124 21 0.0 6 21 125 15 0.0 6 15 126 24 0.0 6 24 127 17 0.0 6 17 128 21 0.0 6 21 129 16 0.0 6 16 130 19 0.0 6 19 131 23 0.0 6 23 132 14 0.0 6 14 133 18 0.0 6 18 134 14 0.0 6 14 135 6 0.0 6 6 136 19 0.0 6 19 137 25 0.0 6 25 138 7 0.0 6 7 139 26 0.0 6 26 140 36 0.0 6 36 141 47 0.0 6 47 142 17 0.0 6 17 143 14 0.0 6 14 144 22 0.0 6 22 145 16 0.0 6 16 146 19 0.0 6 19 147 14 0.0 6 14 148 11 0.0 6 11 149 17 0.0 6 17 150 20 0.0 6 20 151 20 0.0 6 20 152 8 0.0 6 8 153 14 0.0 6 14 154 18 0.0 6 18 155 18 0.0 6 18 156 18 0.0 6 17 1 157 18 0.0 6 18 158 17 0.0 6 17 159 27 0.0 6 27 160 21 0.0 6 21 161 21 0.0 6 21 162 8 0.0 6 8 163 14 0.0 6 14 164 22 0.0 6 22 165 17 0.0 6 16 1 166 12 0.0 6 11 0 0 0 0 0 1 167 19 0.0 6 19 168 11 0.0 6 11 169 17 0.0 6 17 170 15 0.0 6 15 171 12 0.0 6 12 172 16 0.0 6 16 173 22 0.0 6 21 0 0 0 0 0 1 174 10 0.0 6 10 175 17 0.0 6 17 176 20 0.0 6 20 177 12 0.0 6 12 178 20 0.0 6 20 179 12 0.0 6 12 180 11 0.0 6 11 181 12 0.0 6 11 1 182 11 0.0 6 11 183 8 0.0 6 8 184 15 0.0 6 15 185 18 0.0 6 18 186 20 0.0 6 20 187 5 0.0 6 5 188 21 0.0 6 21 189 27 0.0 6 27 190 11 0.0 6 11 191 20 0.0 6 20 192 23 0.0 6 23 193 12 0.0 6 12 194 5 0.0 6 5 195 8 0.0 6 8 196 14 0.0 6 14 197 13 0.0 6 13 198 12 0.0 6 12 199 10 0.0 6 10 200 14 0.0 6 14 201 16 0.0 6 16 202 9 0.0 6 9 203 10 0.0 6 10 204 4 0.0 6 4 205 15 0.0 6 15 206 12 0.0 6 12 207 5 0.0 6 5 208 11 0.0 6 11 209 5 0.0 6 5 210 2 0.0 6 2 211 4 0.0 6 4 212 12 0.0 6 12 213 14 0.0 6 14 214 8 0.0 6 8 215 4 0.0 6 4 216 5 0.0 6 5 217 7 0.0 6 7 218 5 0.0 6 5 219 3 0.0 6 3 220 4 0.0 6 4 221 9 0.0 6 9 222 8 0.0 6 8 223 10 0.0 6 10 224 7 0.0 6 7 225 9 0.0 6 9 226 4 0.0 6 4 227 5 0.0 6 5 228 5 0.0 6 5 229 4 0.0 6 4 230 5 0.0 6 5 231 6 0.0 6 6 232 8 0.0 6 8 233 9 0.0 6 9 234 12 0.0 6 12 235 9 0.0 6 9 236 9 0.0 6 9 237 2 0.0 6 2 238 3 0.0 6 3 239 4 0.0 6 4 240 8 0.0 6 8 241 8 0.0 6 8 242 7 0.0 6 7 243 2 0.0 6 2 244 3 0.0 6 3 245 6 0.0 6 5 1 246 4 0.0 6 4 247 6 0.0 6 6 248 3 0.0 6 3 249 1 0.0 6 1 250 1 0.0 6 1 251 4 0.0 6 4 252 4 0.0 6 4 253 6 0.0 6 6 254 8 0.0 6 8 255 4 0.0 6 4 256 1 0.0 6 1 257 4 0.0 6 4 258 3 0.0 6 3 259 1 0.0 6 1 260 2 0.0 6 2 261 3 0.0 6 3 262 2 0.0 6 2 263 1 0.0 6 1 264 1 0.0 6 1 265 1 0.0 6 1 267 3 0.0 6 3 268 1 0.0 6 1 269 1 0.0 6 1 270 2 0.0 6 2 271 4 0.0 6 4 272 3 0.0 6 3 273 2 0.0 6 2 276 2 0.0 6 2 280 2 0.0 6 2 281 1 0.0 6 1 285 1 0.0 6 1 288 2 0.0 6 2 289 3 0.0 6 3 292 1 0.0 6 1 294 1 0.0 6 1 295 1 0.0 6 1 301 2 0.0 6 2 302 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 73223 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1182.5 0 0 1 6 1 18.5 1 1 7 1 4.6 2 1 10 5 0.1 3 4 1 11 2 0.0 3 2 12 1 0.0 3 1 13 6 0.0 3 6 14 8 0.0 4 7 1 15 1 0.0 4 0 1 16 11 0.0 4 9 2 17 26 0.0 5 22 4 18 27 0.0 5 21 6 19 13 0.0 5 10 1 2 20 113 0.0 6 109 3 1 21 60 0.0 6 57 3 22 34 0.0 6 30 4 23 38 0.0 6 36 2 24 29 0.0 6 27 1 1 25 21 0.0 6 21 26 21 0.0 6 17 3 0 1 27 14 0.0 6 13 1 28 24 0.0 6 23 0 1 29 21 0.0 6 21 30 18 0.0 6 14 4 31 20 0.0 6 19 1 32 16 0.0 6 16 33 12 0.0 6 12 34 12 0.0 6 9 3 35 18 0.0 6 17 1 36 10 0.0 6 9 0 0 0 1 37 13 0.0 6 13 38 17 0.0 6 16 1 39 15 0.0 6 14 1 40 16 0.0 6 13 3 41 19 0.0 6 17 2 42 15 0.0 6 14 1 43 11 0.0 6 9 1 1 44 19 0.0 6 17 2 45 22 0.0 6 20 2 46 15 0.0 6 11 4 47 17 0.0 6 16 1 48 19 0.0 6 19 49 14 0.0 6 13 1 50 22 0.0 6 22 51 16 0.0 6 11 3 2 52 27 0.0 6 26 1 53 30 0.0 6 27 2 0 0 1 54 10 0.0 6 8 2 55 19 0.0 6 15 4 56 15 0.0 6 14 1 57 18 0.0 6 18 58 18 0.0 6 18 59 15 0.0 6 15 60 25 0.0 6 25 61 17 0.0 6 16 1 62 21 0.0 6 19 2 63 37 0.0 6 34 3 64 30 0.0 6 28 1 1 65 36 0.0 6 33 3 66 33 0.0 6 31 2 67 134 0.0 6 127 5 2 68 58 0.0 6 55 3 69 68 0.0 6 63 4 0 1 70 44 0.0 6 43 1 71 52 0.0 6 50 2 72 21 0.0 6 20 1 73 33 0.0 6 29 3 1 74 35 0.0 6 35 75 35 0.0 6 32 3 76 36 0.0 6 36 77 40 0.0 6 36 3 1 78 36 0.0 6 34 0 1 0 0 0 1 79 64 0.0 6 62 2 80 45 0.0 6 42 3 81 54 0.0 6 53 1 82 77 0.0 6 74 3 83 85 0.0 6 77 7 1 84 41 0.0 6 41 85 38 0.0 6 36 2 86 49 0.0 6 47 2 87 47 0.0 6 45 1 0 0 1 88 53 0.0 6 51 2 89 51 0.0 6 50 1 90 58 0.0 6 50 5 1 2 91 60 0.0 6 58 1 0 1 92 67 0.0 6 65 2 93 80 0.0 6 75 4 1 94 54 0.0 6 50 2 0 0 1 1 95 93 0.0 6 88 5 96 66 0.0 6 63 3 97 62 0.0 6 54 5 3 98 77 0.0 6 70 5 1 1 99 71 0.0 6 66 5 100 69 0.0 6 66 3 101 88 0.0 6 85 3 102 80 0.0 6 79 1 103 99 0.0 6 98 1 104 135 0.0 6 133 2 105 87 0.0 6 80 6 0 1 106 86 0.0 6 81 4 0 0 1 107 95 0.0 6 92 3 108 94 0.0 6 92 2 109 90 0.0 6 84 5 1 110 92 0.0 6 85 6 0 0 1 111 116 0.0 6 109 5 1 1 112 147 0.0 6 141 6 113 131 0.0 6 126 3 0 1 0 1 114 130 0.0 6 123 6 0 0 0 0 1 115 134 0.0 6 128 5 1 116 137 0.0 6 133 3 0 1 117 153 0.0 6 147 5 0 0 1 118 156 0.0 6 155 0 0 1 119 161 0.0 6 156 3 1 0 0 0 1 120 148 0.0 6 145 2 1 121 164 0.0 6 160 2 0 0 1 0 1 122 171 0.0 6 164 7 123 198 0.0 6 196 1 0 0 0 0 1 124 227 0.0 6 219 5 0 2 0 1 125 254 0.0 6 244 10 126 226 0.0 6 219 7 127 253 0.0 6 245 7 1 128 320 0.0 6 312 7 0 0 0 0 1 129 337 0.0 6 323 12 1 0 1 130 309 0.0 6 303 5 0 1 131 366 0.0 6 351 12 0 0 1 1 1 132 395 0.0 6 383 10 0 0 0 0 2 133 421 0.0 6 410 10 0 0 1 134 453 0.0 6 444 9 135 427 0.0 6 416 8 1 1 0 0 1 136 388 0.0 6 378 8 0 1 0 1 137 445 0.0 6 436 6 1 1 0 0 1 138 476 0.0 6 465 10 0 0 0 1 139 514 0.0 6 507 7 140 694 0.0 6 678 16 141 721 0.0 6 704 13 1 1 0 1 1 142 874 0.0 6 861 12 0 0 0 1 143 821 0.0 6 812 9 144 911 0.0 6 892 14 1 2 0 2 145 1000 0.0 6 989 10 1 146 1054 0.0 6 1044 9 0 0 0 0 1 147 984 0.0 6 972 11 0 1 148 1110 0.0 6 1091 14 2 1 0 0 2 149 1183 0.0 6 1160 15 4 1 2 1 150 1625 0.0 6 1601 17 3 1 1 2 151 1195 0.0 6 1182 10 1 0 2 152 1475 0.0 6 1445 25 0 2 3 153 1374 0.0 6 1350 19 2 0 2 0 1 154 1118 0.0 6 1100 13 0 0 2 2 1 155 1261 0.0 6 1247 12 0 0 0 0 2 156 1107 0.0 6 1094 10 1 1 0 1 157 1227 0.0 6 1218 8 0 1 158 1370 0.0 6 1357 11 1 0 1 159 1141 0.0 6 1126 12 0 0 0 1 2 160 1261 0.0 6 1249 10 1 0 0 0 1 161 1201 0.0 6 1185 10 0 1 4 1 162 1048 0.0 6 1034 12 0 1 1 163 1096 0.0 6 1084 9 0 1 0 1 1 164 1049 0.0 6 1035 11 1 1 1 165 996 0.0 6 982 13 1 166 1200 0.0 6 1185 10 1 1 2 0 1 167 1015 0.0 6 1005 10 168 1237 0.0 6 1221 16 169 1053 0.0 6 1041 12 170 1059 0.0 6 1048 10 0 0 0 1 171 1234 0.0 6 1220 12 1 1 172 1212 0.0 6 1203 7 1 0 1 173 1220 0.0 6 1210 6 1 2 1 174 1071 0.0 6 1059 9 0 2 0 0 1 175 1078 0.0 6 1064 10 1 1 1 1 176 1121 0.0 6 1108 11 0 1 0 1 177 946 0.0 6 938 8 178 989 0.0 6 980 8 0 0 0 1 179 801 0.0 6 791 10 180 715 0.0 6 710 4 0 0 0 0 1 181 727 0.0 6 725 2 182 771 0.0 6 767 4 183 1038 0.0 6 1027 10 1 184 1211 0.0 6 1203 7 0 0 1 185 1028 0.0 6 1021 7 186 728 0.0 6 724 4 187 704 0.0 6 696 6 1 1 188 694 0.0 6 687 7 189 650 0.0 6 644 6 190 593 0.0 6 592 1 191 640 0.0 6 633 5 0 1 0 0 1 192 548 0.0 6 545 3 193 480 0.0 6 476 4 194 477 0.0 6 473 3 0 1 195 385 0.0 6 377 7 1 196 474 0.0 6 466 8 197 401 0.0 6 397 3 0 0 0 1 198 383 0.0 6 381 2 199 347 0.0 6 343 4 200 317 0.0 6 313 4 201 274 0.0 6 271 2 0 1 202 265 0.0 6 261 4 203 230 0.0 6 225 4 0 1 204 235 0.0 6 233 2 205 219 0.0 6 217 2 206 194 0.0 6 190 4 207 200 0.0 6 198 1 1 208 158 0.0 6 157 1 209 172 0.0 6 171 0 0 1 210 132 0.0 6 132 211 136 0.0 6 136 212 138 0.0 6 137 1 213 139 0.0 6 139 214 182 0.0 6 179 3 215 128 0.0 6 126 2 216 125 0.0 6 125 217 104 0.0 6 103 1 218 98 0.0 6 96 1 1 219 65 0.0 6 65 220 62 0.0 6 62 221 77 0.0 6 76 1 222 65 0.0 6 63 2 223 51 0.0 6 50 1 224 35 0.0 6 35 225 38 0.0 6 38 226 34 0.0 6 34 227 44 0.0 6 43 1 228 34 0.0 6 33 1 229 22 0.0 6 22 230 28 0.0 6 27 1 231 22 0.0 6 22 232 24 0.0 6 24 233 9 0.0 6 8 1 234 12 0.0 6 12 235 8 0.0 6 8 236 6 0.0 6 6 237 6 0.0 6 6 238 8 0.0 6 8 239 9 0.0 6 9 240 4 0.0 6 4 241 15 0.0 6 15 242 8 0.0 6 8 243 3 0.0 6 3 244 8 0.0 6 8 245 5 0.0 6 5 247 6 0.0 6 6 248 6 0.0 6 6 249 2 0.0 6 2 250 1 0.0 6 1 251 3 0.0 6 3 252 4 0.0 6 4 275 1 0.0 6 1 276 1 0.0 6 1 277 2 0.0 6 2 278 10 0.0 6 10 279 72 0.0 6 70 0 1 0 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2408 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 54 1 0.0 6 0 1 55 112 0.0 6 110 2 59 1 0.0 6 1 71 3 0.0 6 3 72 8 0.0 6 8 73 18 0.0 6 18 74 14 0.0 6 14 75 14 0.0 6 14 76 7 0.0 6 7 77 26 0.0 6 26 78 18 0.0 6 18 79 13 0.0 6 13 80 13 0.0 6 11 1 0 1 81 15 0.0 6 15 82 21 0.0 6 21 83 7 0.0 6 7 84 15 0.0 6 15 85 16 0.0 6 16 86 16 0.0 6 16 87 18 0.0 6 18 88 6 0.0 6 5 0 0 1 89 9 0.0 6 8 0 1 90 14 0.0 6 12 2 91 186 0.0 6 181 5 92 10 0.0 6 10 93 24 0.0 6 24 94 12 0.0 6 11 0 0 1 95 25 0.0 6 25 96 15 0.0 6 15 97 10 0.0 6 10 98 16 0.0 6 15 1 99 9 0.0 6 9 100 7 0.0 6 7 101 16 0.0 6 16 102 7 0.0 6 7 103 6 0.0 6 6 104 11 0.0 6 11 105 13 0.0 6 13 106 9 0.0 6 9 107 20 0.0 6 20 108 20 0.0 6 20 109 7 0.0 6 7 110 23 0.0 6 23 111 14 0.0 6 14 112 7 0.0 6 7 113 11 0.0 6 11 114 17 0.0 6 17 115 11 0.0 6 11 116 12 0.0 6 12 117 19 0.0 6 19 118 11 0.0 6 10 1 119 51 0.0 6 51 120 10 0.0 6 10 121 12 0.0 6 12 122 19 0.0 6 19 123 16 0.0 6 15 0 0 0 1 124 11 0.0 6 10 0 0 0 1 125 20 0.0 6 20 126 22 0.0 6 22 127 11 0.0 6 11 128 23 0.0 6 23 129 14 0.0 6 13 0 0 0 1 130 20 0.0 6 20 131 18 0.0 6 18 132 14 0.0 6 13 0 0 0 1 133 10 0.0 6 10 134 23 0.0 6 23 135 9 0.0 6 9 136 16 0.0 6 16 137 15 0.0 6 15 138 16 0.0 6 16 139 19 0.0 6 19 140 29 0.0 6 29 141 48 0.0 6 48 142 23 0.0 6 23 143 28 0.0 6 27 0 0 0 1 144 12 0.0 6 12 145 11 0.0 6 11 146 14 0.0 6 13 0 0 1 147 18 0.0 6 18 148 19 0.0 6 19 149 27 0.0 6 27 150 21 0.0 6 21 151 21 0.0 6 21 152 8 0.0 6 8 153 20 0.0 6 19 0 0 0 1 154 24 0.0 6 24 155 19 0.0 6 19 156 16 0.0 6 15 0 0 0 1 157 23 0.0 6 23 158 17 0.0 6 17 159 22 0.0 6 22 160 18 0.0 6 18 161 30 0.0 6 30 162 12 0.0 6 9 1 1 0 1 163 14 0.0 6 13 1 164 31 0.0 6 31 165 17 0.0 6 17 166 16 0.0 6 16 167 25 0.0 6 25 168 13 0.0 6 13 169 18 0.0 6 18 170 18 0.0 6 18 171 15 0.0 6 15 172 16 0.0 6 15 0 0 0 1 173 18 0.0 6 17 0 0 1 174 10 0.0 6 10 175 14 0.0 6 14 176 22 0.0 6 22 177 13 0.0 6 13 178 14 0.0 6 14 179 8 0.0 6 8 180 8 0.0 6 8 181 10 0.0 6 10 182 14 0.0 6 14 183 9 0.0 6 9 184 13 0.0 6 13 185 21 0.0 6 21 186 18 0.0 6 18 187 8 0.0 6 8 188 15 0.0 6 15 189 22 0.0 6 22 190 12 0.0 6 12 191 20 0.0 6 20 192 16 0.0 6 16 193 9 0.0 6 9 194 9 0.0 6 9 195 13 0.0 6 12 0 0 0 1 196 4 0.0 6 4 197 4 0.0 6 4 198 3 0.0 6 3 199 8 0.0 6 8 200 8 0.0 6 8 201 12 0.0 6 12 202 4 0.0 6 4 203 6 0.0 6 6 204 4 0.0 6 3 0 0 0 1 206 5 0.0 6 5 207 8 0.0 6 8 208 3 0.0 6 3 209 3 0.0 6 3 210 3 0.0 6 2 0 1 211 1 0.0 6 1 212 5 0.0 6 5 213 5 0.0 6 5 214 1 0.0 6 1 215 1 0.0 6 1 216 3 0.0 6 3 217 2 0.0 6 2 218 3 0.0 6 3 219 3 0.0 6 3 220 2 0.0 6 2 222 1 0.0 6 1 224 1 0.0 6 1 225 2 0.0 6 2 226 3 0.0 6 3 227 1 0.0 6 1 228 1 0.0 6 1 229 1 0.0 6 1 230 1 0.0 6 1 231 5 0.0 6 5 233 1 0.0 6 1 234 2 0.0 6 2 235 1 0.0 6 1 236 1 0.0 6 1 239 3 0.0 6 3 240 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_16_19_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_16_246_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_16_19_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_16_246_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.21 s (21 µs/read; 2.86 M reads/minute). === Summary === Total read pairs processed: 105,264 Read 1 with adapter: 105,255 (100.0%) Read 2 with adapter: 105,225 (100.0%) == Read fate breakdown == Pairs that were too short: 48 (0.0%) Pairs discarded as untrimmed: 46 (0.0%) Pairs written (passing filters): 105,170 (99.9%) Total basepairs processed: 44,981,032 bp Read 1: 24,618,397 bp Read 2: 20,362,635 bp Total written (filtered): 6,842,553 bp (15.2%) Read 1: 3,563,450 bp Read 2: 3,279,103 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 101532 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 8 411.2 1 5 3 5 3 102.8 1 3 6 3 25.7 1 1 0 2 7 6 6.4 2 5 0 1 8 1 1.6 2 1 9 3 0.4 2 0 3 13 1 0.0 3 1 14 2 0.0 4 2 15 3 0.0 4 3 16 3 0.0 4 3 17 3 0.0 5 2 1 18 2 0.0 5 2 19 6 0.0 5 4 2 20 151 0.0 6 149 2 21 68 0.0 6 68 22 63 0.0 6 61 2 23 44 0.0 6 42 1 1 24 28 0.0 6 26 2 25 33 0.0 6 33 26 30 0.0 6 28 2 27 15 0.0 6 14 1 28 13 0.0 6 12 1 29 7 0.0 6 6 1 30 15 0.0 6 15 31 13 0.0 6 13 32 6 0.0 6 5 1 33 7 0.0 6 7 34 7 0.0 6 7 35 8 0.0 6 8 36 4 0.0 6 4 37 3 0.0 6 3 38 9 0.0 6 9 39 7 0.0 6 7 40 3 0.0 6 3 41 11 0.0 6 10 1 42 6 0.0 6 6 43 6 0.0 6 6 44 12 0.0 6 12 45 4 0.0 6 4 46 9 0.0 6 8 1 47 6 0.0 6 6 48 13 0.0 6 13 49 5 0.0 6 3 1 0 1 50 2 0.0 6 2 51 7 0.0 6 7 52 8 0.0 6 8 53 8 0.0 6 8 54 5 0.0 6 5 55 8 0.0 6 8 56 2 0.0 6 2 57 6 0.0 6 6 58 5 0.0 6 5 59 7 0.0 6 7 60 10 0.0 6 10 61 8 0.0 6 6 2 62 11 0.0 6 10 1 63 9 0.0 6 9 64 10 0.0 6 10 65 9 0.0 6 9 66 16 0.0 6 16 67 9 0.0 6 9 68 6 0.0 6 5 1 69 14 0.0 6 13 1 70 9 0.0 6 9 71 14 0.0 6 13 1 72 14 0.0 6 13 1 73 17 0.0 6 15 1 0 0 0 0 1 74 14 0.0 6 13 1 75 15 0.0 6 13 1 1 76 10 0.0 6 10 77 7 0.0 6 7 78 6 0.0 6 6 79 4 0.0 6 4 80 7 0.0 6 7 81 9 0.0 6 8 1 82 10 0.0 6 8 1 1 83 8 0.0 6 8 84 11 0.0 6 10 0 1 85 6 0.0 6 5 1 86 17 0.0 6 15 1 0 0 0 0 1 87 20 0.0 6 18 2 88 16 0.0 6 14 1 1 89 6 0.0 6 6 90 7 0.0 6 7 91 8 0.0 6 7 1 92 10 0.0 6 7 3 93 7 0.0 6 6 0 1 94 9 0.0 6 9 95 12 0.0 6 12 96 10 0.0 6 9 1 97 10 0.0 6 9 1 98 12 0.0 6 11 0 1 99 12 0.0 6 11 1 100 14 0.0 6 14 101 22 0.0 6 21 1 102 21 0.0 6 19 2 103 26 0.0 6 26 104 25 0.0 6 23 2 105 21 0.0 6 20 0 0 0 0 0 1 106 14 0.0 6 12 1 0 0 0 0 1 107 18 0.0 6 17 1 108 21 0.0 6 21 109 31 0.0 6 30 1 110 34 0.0 6 34 111 32 0.0 6 30 2 112 29 0.0 6 29 113 29 0.0 6 28 1 114 24 0.0 6 24 115 17 0.0 6 16 1 116 30 0.0 6 29 1 117 36 0.0 6 36 118 25 0.0 6 23 1 0 0 0 1 119 22 0.0 6 22 120 26 0.0 6 25 1 121 33 0.0 6 31 1 0 0 0 0 1 122 45 0.0 6 44 1 123 38 0.0 6 36 1 0 0 0 1 124 40 0.0 6 40 125 29 0.0 6 29 126 44 0.0 6 41 3 127 40 0.0 6 40 128 49 0.0 6 48 1 129 55 0.0 6 55 130 42 0.0 6 41 1 131 44 0.0 6 44 132 56 0.0 6 55 1 133 55 0.0 6 55 134 63 0.0 6 62 1 135 53 0.0 6 51 2 136 45 0.0 6 45 137 56 0.0 6 56 138 75 0.0 6 74 0 0 0 1 139 106 0.0 6 106 140 157 0.0 6 155 2 141 711 0.0 6 708 3 142 864 0.0 6 863 1 143 1235 0.0 6 1232 3 144 711 0.0 6 709 2 145 1570 0.0 6 1563 7 146 820 0.0 6 811 8 0 0 0 0 1 147 683 0.0 6 682 1 148 633 0.0 6 631 2 149 1045 0.0 6 1038 6 0 0 0 1 150 778 0.0 6 775 3 151 849 0.0 6 846 1 2 152 482 0.0 6 480 2 153 268 0.0 6 266 2 154 630 0.0 6 626 4 155 889 0.0 6 883 5 0 0 1 156 339 0.0 6 334 3 1 0 1 157 254 0.0 6 251 2 0 0 0 1 158 393 0.0 6 391 2 159 498 0.0 6 496 2 160 543 0.0 6 541 1 0 1 161 635 0.0 6 635 162 728 0.0 6 726 1 0 0 0 0 1 163 941 0.0 6 935 4 0 1 0 0 1 164 941 0.0 6 935 5 0 1 165 924 0.0 6 920 3 0 1 166 381 0.0 6 376 4 0 1 167 1155 0.0 6 1153 1 0 1 168 518 0.0 6 516 2 169 308 0.0 6 306 2 170 352 0.0 6 352 171 475 0.0 6 472 3 172 1177 0.0 6 1174 3 173 1041 0.0 6 1035 4 1 0 0 1 174 296 0.0 6 294 2 175 577 0.0 6 574 3 176 832 0.0 6 829 3 177 602 0.0 6 600 2 178 269 0.0 6 264 4 0 0 0 1 179 729 0.0 6 726 3 180 294 0.0 6 290 4 181 550 0.0 6 546 4 182 543 0.0 6 542 1 183 472 0.0 6 469 3 184 485 0.0 6 480 5 185 640 0.0 6 637 1 1 0 0 0 1 186 901 0.0 6 895 5 0 0 0 1 187 410 0.0 6 407 2 0 0 0 1 188 529 0.0 6 525 3 0 0 0 0 1 189 1926 0.0 6 1922 3 0 0 0 1 190 1033 0.0 6 1032 0 0 1 191 339 0.0 6 336 3 192 412 0.0 6 411 0 1 193 435 0.0 6 432 3 194 991 0.0 6 986 4 1 195 3510 0.0 6 3500 7 0 0 0 2 1 196 699 0.0 6 693 3 2 0 0 0 1 197 498 0.0 6 493 4 0 0 0 0 1 198 747 0.0 6 740 5 0 0 0 0 2 199 489 0.0 6 487 2 200 551 0.0 6 546 4 0 0 0 0 1 201 932 0.0 6 926 5 0 1 202 553 0.0 6 548 5 203 656 0.0 6 651 5 204 461 0.0 6 451 5 1 0 0 1 3 205 579 0.0 6 573 6 206 1056 0.0 6 1042 11 0 0 0 2 1 207 969 0.0 6 962 6 0 0 0 1 208 977 0.0 6 971 6 209 848 0.0 6 842 6 210 1029 0.0 6 1019 9 0 0 0 1 211 1381 0.0 6 1374 7 212 1918 0.0 6 1915 3 213 2386 0.0 6 2372 12 1 0 1 214 1228 0.0 6 1220 7 0 0 0 1 215 2784 0.0 6 2770 13 1 216 1031 0.0 6 1024 7 217 717 0.0 6 712 4 0 0 1 218 807 0.0 6 795 10 1 0 0 1 219 918 0.0 6 908 9 0 0 0 1 220 638 0.0 6 634 4 221 500 0.0 6 494 5 0 0 0 1 222 767 0.0 6 763 4 223 652 0.0 6 643 9 224 828 0.0 6 821 6 0 0 1 225 1168 0.0 6 1165 2 0 0 0 1 226 966 0.0 6 958 7 0 0 1 227 946 0.0 6 940 5 0 0 0 0 1 228 1457 0.0 6 1449 8 229 844 0.0 6 841 3 230 609 0.0 6 604 5 231 696 0.0 6 694 1 1 232 665 0.0 6 659 5 0 1 233 772 0.0 6 770 2 234 1067 0.0 6 1055 10 2 235 637 0.0 6 637 236 693 0.0 6 688 4 0 0 0 1 237 843 0.0 6 838 5 238 626 0.0 6 617 8 1 239 546 0.0 6 544 1 0 0 0 0 1 240 699 0.0 6 697 2 241 715 0.0 6 713 1 1 242 1133 0.0 6 1127 6 243 1650 0.0 6 1646 4 244 445 0.0 6 443 1 1 245 576 0.0 6 573 3 246 1037 0.0 6 1034 2 0 0 0 1 247 295 0.0 6 294 1 248 332 0.0 6 330 1 0 0 0 1 249 372 0.0 6 367 4 0 0 1 250 267 0.0 6 265 1 0 0 0 0 1 251 263 0.0 6 259 3 0 0 1 252 271 0.0 6 268 2 0 1 253 293 0.0 6 290 2 0 0 0 0 1 254 259 0.0 6 259 255 337 0.0 6 335 2 256 209 0.0 6 207 1 0 0 0 1 257 257 0.0 6 256 1 258 311 0.0 6 307 4 259 267 0.0 6 265 2 260 279 0.0 6 273 6 261 209 0.0 6 206 3 262 121 0.0 6 121 263 180 0.0 6 177 2 0 0 1 264 187 0.0 6 185 2 265 154 0.0 6 153 0 0 0 0 0 1 266 193 0.0 6 192 1 267 190 0.0 6 188 1 0 0 0 1 268 197 0.0 6 196 1 269 219 0.0 6 217 2 270 202 0.0 6 201 1 271 218 0.0 6 214 4 272 181 0.0 6 180 1 273 219 0.0 6 216 2 0 0 1 274 258 0.0 6 257 1 275 384 0.0 6 381 2 0 0 0 1 276 679 0.0 6 677 2 277 458 0.0 6 457 1 278 1287 0.0 6 1272 14 0 1 279 2267 0.0 6 2257 9 0 0 0 0 1 280 10 0.0 6 10 281 1 0.0 6 1 282 1 0.0 6 1 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 3723 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 1 0.0 6 1 70 2 0.0 6 2 71 5 0.0 6 5 72 37 0.0 6 37 73 38 0.0 6 38 74 48 0.0 6 48 75 39 0.0 6 39 76 84 0.0 6 84 77 28 0.0 6 28 78 29 0.0 6 28 1 79 30 0.0 6 30 80 54 0.0 6 54 81 40 0.0 6 40 82 29 0.0 6 29 83 15 0.0 6 15 84 9 0.0 6 9 85 33 0.0 6 33 86 37 0.0 6 37 87 6 0.0 6 6 88 9 0.0 6 9 89 13 0.0 6 13 90 17 0.0 6 17 91 21 0.0 6 21 92 28 0.0 6 28 93 37 0.0 6 36 1 94 42 0.0 6 42 95 43 0.0 6 43 96 25 0.0 6 24 1 97 24 0.0 6 24 98 54 0.0 6 54 99 22 0.0 6 22 100 10 0.0 6 10 101 10 0.0 6 10 102 25 0.0 6 25 103 60 0.0 6 60 104 42 0.0 6 42 105 10 0.0 6 10 106 20 0.0 6 20 107 32 0.0 6 32 108 19 0.0 6 19 109 5 0.0 6 5 110 26 0.0 6 26 111 6 0.0 6 6 112 18 0.0 6 18 113 14 0.0 6 14 114 12 0.0 6 12 115 11 0.0 6 11 116 19 0.0 6 19 117 37 0.0 6 37 118 10 0.0 6 10 119 37 0.0 6 37 120 100 0.0 6 98 2 121 39 0.0 6 38 1 122 3 0.0 6 3 123 3 0.0 6 3 124 12 0.0 6 11 1 125 61 0.0 6 61 126 138 0.0 6 138 127 6 0.0 6 5 1 128 3 0.0 6 3 129 11 0.0 6 11 130 2 0.0 6 2 131 9 0.0 6 8 0 0 0 0 0 1 132 20 0.0 6 20 133 21 0.0 6 21 134 16 0.0 6 16 135 1 0.0 6 0 0 0 0 0 0 1 136 5 0.0 6 5 137 10 0.0 6 10 138 9 0.0 6 9 139 4 0.0 6 4 140 9 0.0 6 9 141 23 0.0 6 23 142 29 0.0 6 29 143 77 0.0 6 77 144 38 0.0 6 38 145 49 0.0 6 49 146 111 0.0 6 111 147 5 0.0 6 5 148 9 0.0 6 9 149 5 0.0 6 5 150 26 0.0 6 26 151 8 0.0 6 8 153 2 0.0 6 2 154 2 0.0 6 2 155 15 0.0 6 15 156 25 0.0 6 25 157 2 0.0 6 2 158 42 0.0 6 42 159 113 0.0 6 113 160 47 0.0 6 46 1 161 7 0.0 6 7 162 16 0.0 6 16 163 14 0.0 6 14 164 23 0.0 6 23 165 65 0.0 6 64 1 166 2 0.0 6 2 168 1 0.0 6 1 169 8 0.0 6 8 170 21 0.0 6 21 171 1 0.0 6 1 172 6 0.0 6 6 173 10 0.0 6 10 174 6 0.0 6 6 175 13 0.0 6 13 176 52 0.0 6 52 177 155 0.0 6 155 178 11 0.0 6 11 179 25 0.0 6 25 180 33 0.0 6 33 181 1 0.0 6 0 0 0 0 0 0 1 185 2 0.0 6 2 186 20 0.0 6 20 187 1 0.0 6 1 188 12 0.0 6 12 189 7 0.0 6 7 192 1 0.0 6 1 193 4 0.0 6 4 197 4 0.0 6 4 198 12 0.0 6 12 199 13 0.0 6 13 200 5 0.0 6 5 201 33 0.0 6 33 202 14 0.0 6 14 203 7 0.0 6 7 204 25 0.0 6 25 205 12 0.0 6 12 206 57 0.0 6 56 1 207 194 0.0 6 194 208 10 0.0 6 10 209 15 0.0 6 15 210 58 0.0 6 58 211 4 0.0 6 4 212 8 0.0 6 8 213 2 0.0 6 2 214 7 0.0 6 7 215 21 0.0 6 20 1 216 7 0.0 6 7 217 15 0.0 6 15 218 4 0.0 6 4 219 17 0.0 6 17 221 1 0.0 6 1 222 12 0.0 6 12 223 2 0.0 6 2 224 9 0.0 6 9 225 29 0.0 6 29 226 4 0.0 6 4 227 3 0.0 6 3 228 1 0.0 6 1 229 9 0.0 6 9 232 2 0.0 6 2 237 3 0.0 6 3 241 7 0.0 6 7 242 10 0.0 6 10 243 1 0.0 6 1 244 2 0.0 6 2 245 5 0.0 6 5 246 4 0.0 6 4 247 6 0.0 6 6 249 1 0.0 6 1 251 1 0.0 6 1 253 4 0.0 6 4 254 3 0.0 6 3 255 3 0.0 6 3 256 2 0.0 6 2 257 6 0.0 6 6 258 29 0.0 6 29 259 8 0.0 6 8 260 3 0.0 6 3 261 3 0.0 6 3 263 1 0.0 6 1 264 2 0.0 6 2 265 4 0.0 6 4 266 11 0.0 6 11 267 5 0.0 6 5 268 1 0.0 6 1 269 1 0.0 6 1 271 1 0.0 6 1 273 2 0.0 6 2 277 1 0.0 6 1 278 1 0.0 6 1 279 1 0.0 6 1 288 2 0.0 6 2 289 2 0.0 6 2 293 5 0.0 6 5 298 3 0.0 6 3 299 2 0.0 6 2 300 3 0.0 6 3 301 4 0.0 6 4 302 1 0.0 6 1 306 1 0.0 6 1 307 3 0.0 6 3 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 101662 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 6.4 2 0 1 9 1 0.4 2 1 11 6 0.0 3 5 1 12 3 0.0 3 3 13 12 0.0 3 12 14 3 0.0 4 3 15 2 0.0 4 2 16 18 0.0 4 16 2 17 15 0.0 5 13 2 18 11 0.0 5 11 19 9 0.0 5 8 1 20 125 0.0 6 121 4 21 73 0.0 6 70 3 22 56 0.0 6 53 3 23 32 0.0 6 31 1 24 22 0.0 6 20 2 25 29 0.0 6 28 1 26 18 0.0 6 16 2 27 23 0.0 6 20 2 0 0 1 28 16 0.0 6 15 1 29 19 0.0 6 18 1 30 19 0.0 6 19 31 15 0.0 6 15 32 17 0.0 6 17 33 18 0.0 6 16 1 0 1 34 10 0.0 6 8 2 35 17 0.0 6 17 36 8 0.0 6 8 37 9 0.0 6 9 38 7 0.0 6 7 39 15 0.0 6 12 2 1 40 11 0.0 6 10 1 41 17 0.0 6 17 42 15 0.0 6 14 1 43 15 0.0 6 13 2 44 19 0.0 6 16 3 45 12 0.0 6 10 2 46 11 0.0 6 11 47 12 0.0 6 12 48 22 0.0 6 19 3 49 20 0.0 6 20 50 11 0.0 6 10 1 51 12 0.0 6 10 2 52 19 0.0 6 18 1 53 19 0.0 6 19 54 22 0.0 6 19 3 55 21 0.0 6 17 2 1 1 56 24 0.0 6 21 3 57 21 0.0 6 20 1 58 23 0.0 6 21 2 59 24 0.0 6 24 60 29 0.0 6 28 1 61 23 0.0 6 23 62 26 0.0 6 25 1 63 15 0.0 6 15 64 32 0.0 6 28 2 1 0 0 0 1 65 37 0.0 6 34 2 1 66 37 0.0 6 32 5 67 28 0.0 6 24 4 68 47 0.0 6 42 5 69 207 0.0 6 197 10 70 68 0.0 6 65 3 71 92 0.0 6 87 5 72 47 0.0 6 46 1 73 64 0.0 6 59 3 1 1 74 37 0.0 6 36 1 75 30 0.0 6 29 1 76 59 0.0 6 55 4 77 33 0.0 6 32 0 1 78 44 0.0 6 41 3 79 38 0.0 6 35 3 80 36 0.0 6 35 0 1 81 56 0.0 6 55 1 82 47 0.0 6 45 2 83 45 0.0 6 43 2 84 68 0.0 6 61 5 0 1 0 1 85 87 0.0 6 84 3 86 36 0.0 6 34 2 87 47 0.0 6 44 3 88 63 0.0 6 62 1 89 58 0.0 6 57 1 90 56 0.0 6 52 3 0 1 91 61 0.0 6 56 5 92 53 0.0 6 52 1 93 56 0.0 6 55 1 94 69 0.0 6 66 2 0 0 1 95 75 0.0 6 71 3 0 1 96 58 0.0 6 56 2 97 72 0.0 6 71 1 98 93 0.0 6 92 1 99 76 0.0 6 74 2 100 88 0.0 6 83 3 2 101 72 0.0 6 70 1 1 102 71 0.0 6 68 2 0 0 0 0 1 103 91 0.0 6 88 2 0 0 0 0 1 104 86 0.0 6 80 5 1 105 106 0.0 6 100 6 106 155 0.0 6 149 4 1 0 0 1 107 120 0.0 6 115 4 0 0 0 1 108 118 0.0 6 113 3 1 1 109 123 0.0 6 114 6 1 1 1 110 111 0.0 6 106 3 1 0 1 111 110 0.0 6 109 0 0 0 0 0 1 112 151 0.0 6 147 4 113 153 0.0 6 145 8 114 168 0.0 6 166 1 0 1 115 141 0.0 6 136 2 2 0 0 0 1 116 188 0.0 6 177 9 0 1 1 117 186 0.0 6 182 3 0 1 118 164 0.0 6 161 2 0 0 0 0 1 119 193 0.0 6 188 5 120 174 0.0 6 169 4 0 0 0 1 121 179 0.0 6 173 5 1 122 212 0.0 6 209 1 0 1 1 123 167 0.0 6 166 1 124 212 0.0 6 205 7 125 250 0.0 6 244 4 1 0 0 0 1 126 239 0.0 6 231 7 0 0 0 0 1 127 314 0.0 6 307 7 128 329 0.0 6 320 9 129 310 0.0 6 303 5 1 0 0 1 130 375 0.0 6 369 5 0 0 0 1 131 351 0.0 6 345 4 0 2 132 323 0.0 6 317 5 0 0 0 0 1 133 388 0.0 6 382 6 134 483 0.0 6 476 6 1 135 517 0.0 6 507 10 136 599 0.0 6 589 8 0 1 1 137 548 0.0 6 546 2 138 629 0.0 6 621 6 0 1 0 0 1 139 694 0.0 6 684 8 1 1 140 1170 0.0 6 1158 11 1 141 1237 0.0 6 1218 17 1 0 0 1 142 1444 0.0 6 1435 9 143 1291 0.0 6 1274 14 1 0 2 144 1983 0.0 6 1967 15 0 0 1 145 1346 0.0 6 1331 14 1 146 1317 0.0 6 1297 19 0 0 0 1 147 1187 0.0 6 1171 12 0 3 0 1 148 1591 0.0 6 1580 9 1 0 0 1 149 1529 0.0 6 1501 19 1 2 5 0 1 150 1567 0.0 6 1550 15 1 1 151 1465 0.0 6 1452 12 0 0 0 0 1 152 1810 0.0 6 1779 29 1 0 0 1 153 1592 0.0 6 1573 13 1 0 3 0 2 154 1993 0.0 6 1964 28 0 1 155 1611 0.0 6 1591 16 1 0 1 1 1 156 1296 0.0 6 1273 22 0 0 0 1 157 1584 0.0 6 1560 20 2 0 1 1 158 1545 0.0 6 1521 22 0 0 2 159 1681 0.0 6 1660 18 1 1 0 1 160 1785 0.0 6 1768 15 1 1 161 1545 0.0 6 1521 17 4 2 0 0 1 162 1812 0.0 6 1796 13 1 1 1 163 1397 0.0 6 1388 7 0 0 2 164 1428 0.0 6 1407 17 2 1 0 1 165 1352 0.0 6 1331 19 1 1 166 1144 0.0 6 1134 9 0 0 1 167 1199 0.0 6 1171 24 2 0 1 1 168 1619 0.0 6 1598 17 2 0 1 1 169 1357 0.0 6 1344 13 170 1911 0.0 6 1888 21 0 1 0 0 1 171 1709 0.0 6 1697 11 0 0 0 0 1 172 1512 0.0 6 1492 18 1 0 1 173 1621 0.0 6 1592 27 1 0 0 1 174 1656 0.0 6 1640 14 0 1 1 175 1444 0.0 6 1431 9 1 0 0 2 1 176 1399 0.0 6 1387 10 0 1 0 1 177 1374 0.0 6 1366 8 178 1393 0.0 6 1384 8 1 179 1061 0.0 6 1049 9 0 1 2 180 1118 0.0 6 1109 9 181 882 0.0 6 874 8 182 873 0.0 6 865 7 0 0 0 1 183 886 0.0 6 877 8 0 1 184 827 0.0 6 818 9 185 1069 0.0 6 1064 5 186 1765 0.0 6 1749 13 2 0 1 187 1809 0.0 6 1794 12 1 0 2 188 1591 0.0 6 1580 8 0 0 2 0 1 189 1177 0.0 6 1165 10 0 2 190 1029 0.0 6 1018 8 0 0 3 191 1083 0.0 6 1077 5 0 0 1 192 968 0.0 6 959 8 0 0 1 193 1003 0.0 6 998 4 1 194 1104 0.0 6 1100 4 195 776 0.0 6 770 5 1 196 520 0.0 6 520 197 589 0.0 6 582 7 198 725 0.0 6 723 2 199 1014 0.0 6 1010 4 200 588 0.0 6 581 5 0 1 0 0 1 201 483 0.0 6 477 3 1 0 0 1 1 202 382 0.0 6 380 2 203 368 0.0 6 364 4 204 387 0.0 6 384 3 205 333 0.0 6 329 4 206 389 0.0 6 387 2 207 385 0.0 6 381 4 208 321 0.0 6 319 1 0 0 0 1 209 284 0.0 6 280 4 210 345 0.0 6 342 3 211 354 0.0 6 351 3 212 227 0.0 6 226 0 0 0 1 213 314 0.0 6 312 1 0 0 0 1 214 411 0.0 6 410 1 215 264 0.0 6 261 3 216 368 0.0 6 367 1 217 156 0.0 6 154 1 0 0 1 218 148 0.0 6 144 3 0 0 0 0 1 219 155 0.0 6 153 2 220 108 0.0 6 107 1 221 134 0.0 6 131 2 0 0 0 1 222 63 0.0 6 62 1 223 80 0.0 6 79 0 0 0 0 1 224 117 0.0 6 117 225 56 0.0 6 56 226 52 0.0 6 52 227 70 0.0 6 68 2 228 51 0.0 6 49 2 229 46 0.0 6 44 2 230 32 0.0 6 32 231 31 0.0 6 31 232 21 0.0 6 21 233 20 0.0 6 20 234 18 0.0 6 18 235 22 0.0 6 22 236 11 0.0 6 11 237 21 0.0 6 21 238 17 0.0 6 17 239 7 0.0 6 6 1 240 22 0.0 6 21 1 241 14 0.0 6 14 242 4 0.0 6 4 243 5 0.0 6 5 244 6 0.0 6 6 245 3 0.0 6 3 246 2 0.0 6 2 247 1 0.0 6 1 248 2 0.0 6 2 249 3 0.0 6 3 250 7 0.0 6 7 251 1 0.0 6 1 252 2 0.0 6 2 253 6 0.0 6 5 1 254 2 0.0 6 2 255 1 0.0 6 1 278 1 0.0 6 1 280 13 0.0 6 13 281 91 0.0 6 90 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 3563 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 47 1 0.0 6 0 1 53 1 0.0 6 1 64 1 0.0 6 0 1 66 1 0.0 6 1 69 7 0.0 6 7 70 39 0.0 6 39 71 36 0.0 6 36 72 63 0.0 6 63 73 32 0.0 6 32 74 67 0.0 6 67 75 25 0.0 6 24 1 76 40 0.0 6 40 77 25 0.0 6 25 78 47 0.0 6 47 79 35 0.0 6 35 80 43 0.0 6 43 81 14 0.0 6 13 1 82 10 0.0 6 10 83 36 0.0 6 36 84 54 0.0 6 53 0 0 0 1 85 7 0.0 6 6 1 86 13 0.0 6 13 87 21 0.0 6 20 0 0 0 1 88 13 0.0 6 13 89 13 0.0 6 13 90 24 0.0 6 24 91 33 0.0 6 32 1 92 51 0.0 6 51 93 45 0.0 6 44 1 94 36 0.0 6 36 95 21 0.0 6 21 96 59 0.0 6 58 0 0 0 1 97 20 0.0 6 20 98 3 0.0 6 3 99 10 0.0 6 10 100 28 0.0 6 28 101 68 0.0 6 68 102 40 0.0 6 39 1 103 10 0.0 6 10 104 32 0.0 6 31 0 0 0 1 105 34 0.0 6 34 106 20 0.0 6 19 0 0 0 1 107 8 0.0 6 8 108 26 0.0 6 26 109 9 0.0 6 9 110 26 0.0 6 26 111 14 0.0 6 14 112 9 0.0 6 9 113 16 0.0 6 15 0 0 1 114 27 0.0 6 27 115 41 0.0 6 41 116 3 0.0 6 3 117 27 0.0 6 26 0 0 0 1 118 107 0.0 6 107 119 29 0.0 6 29 120 1 0.0 6 1 121 1 0.0 6 1 122 11 0.0 6 11 123 50 0.0 6 49 0 0 0 1 124 190 0.0 6 190 125 3 0.0 6 3 126 4 0.0 6 4 127 17 0.0 6 17 128 1 0.0 6 0 0 0 0 1 129 6 0.0 6 6 130 26 0.0 6 26 131 10 0.0 6 10 132 15 0.0 6 15 134 3 0.0 6 2 0 0 0 1 135 13 0.0 6 13 136 13 0.0 6 13 137 8 0.0 6 8 138 8 0.0 6 8 139 27 0.0 6 27 140 32 0.0 6 31 0 0 0 1 141 80 0.0 6 80 142 54 0.0 6 54 143 35 0.0 6 34 0 0 0 1 144 155 0.0 6 154 0 0 0 1 145 3 0.0 6 3 146 5 0.0 6 4 0 0 0 1 147 5 0.0 6 5 148 29 0.0 6 29 149 21 0.0 6 21 150 2 0.0 6 2 152 2 0.0 6 2 153 23 0.0 6 23 154 31 0.0 6 31 155 2 0.0 6 2 156 24 0.0 6 24 157 166 0.0 6 166 158 64 0.0 6 62 0 0 0 2 159 4 0.0 6 4 160 26 0.0 6 23 1 0 0 1 0 1 161 16 0.0 6 15 0 0 0 1 162 21 0.0 6 21 163 84 0.0 6 84 164 5 0.0 6 5 166 2 0.0 6 2 167 7 0.0 6 7 168 18 0.0 6 18 171 7 0.0 6 6 1 172 9 0.0 6 9 173 6 0.0 6 6 174 25 0.0 6 25 175 177 0.0 6 177 176 7 0.0 6 7 177 20 0.0 6 20 178 42 0.0 6 42 179 1 0.0 6 1 180 1 0.0 6 0 0 0 1 181 1 0.0 6 1 182 1 0.0 6 1 183 4 0.0 6 4 184 23 0.0 6 23 185 3 0.0 6 3 186 10 0.0 6 10 187 15 0.0 6 15 190 1 0.0 6 1 193 1 0.0 6 1 196 10 0.0 6 10 197 3 0.0 6 3 198 1 0.0 6 1 199 18 0.0 6 18 200 12 0.0 6 12 201 2 0.0 6 2 202 12 0.0 6 12 203 10 0.0 6 10 204 35 0.0 6 35 205 162 0.0 6 162 206 2 0.0 6 2 207 6 0.0 6 6 208 21 0.0 6 21 209 1 0.0 6 1 210 6 0.0 6 5 1 212 1 0.0 6 1 213 5 0.0 6 5 214 4 0.0 6 4 215 11 0.0 6 11 216 2 0.0 6 2 217 2 0.0 6 2 220 4 0.0 6 4 222 4 0.0 6 4 223 20 0.0 6 20 224 2 0.0 6 1 1 225 4 0.0 6 4 227 3 0.0 6 3 239 1 0.0 6 1 243 1 0.0 6 1 259 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_17_20_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_17_247_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_17_20_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_17_247_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.81 s (30 µs/read; 2.00 M reads/minute). === Summary === Total read pairs processed: 60,337 Read 1 with adapter: 60,326 (100.0%) Read 2 with adapter: 60,318 (100.0%) == Read fate breakdown == Pairs that were too short: 9 (0.0%) Pairs discarded as untrimmed: 29 (0.0%) Pairs written (passing filters): 60,299 (99.9%) Total basepairs processed: 23,456,942 bp Read 1: 12,268,568 bp Read 2: 11,188,374 bp Total written (filtered): 3,974,932 bp (16.9%) Read 1: 2,083,504 bp Read 2: 1,891,428 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 57910 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 942.8 0 0 2 4 2 235.7 1 2 6 1 14.7 1 0 0 1 7 2 3.7 2 2 9 2 0.2 2 1 1 16 1 0.0 4 1 17 5 0.0 5 5 18 2 0.0 5 2 19 2 0.0 5 2 20 59 0.0 6 58 1 21 34 0.0 6 33 1 22 29 0.0 6 29 23 18 0.0 6 18 24 20 0.0 6 19 1 25 19 0.0 6 17 2 26 23 0.0 6 22 1 27 12 0.0 6 11 1 28 15 0.0 6 13 2 29 9 0.0 6 9 30 7 0.0 6 7 31 10 0.0 6 10 32 8 0.0 6 8 33 6 0.0 6 6 34 17 0.0 6 15 2 35 8 0.0 6 7 1 36 12 0.0 6 11 0 0 0 0 1 37 8 0.0 6 8 38 3 0.0 6 2 1 39 3 0.0 6 3 40 8 0.0 6 7 1 41 12 0.0 6 10 1 0 0 0 1 42 6 0.0 6 5 1 43 3 0.0 6 2 1 44 4 0.0 6 3 1 45 7 0.0 6 7 46 3 0.0 6 3 47 7 0.0 6 7 48 3 0.0 6 3 49 7 0.0 6 7 50 12 0.0 6 12 51 2 0.0 6 2 52 6 0.0 6 6 53 3 0.0 6 3 54 4 0.0 6 3 1 55 2 0.0 6 2 56 4 0.0 6 4 59 3 0.0 6 3 60 2 0.0 6 2 61 4 0.0 6 4 62 2 0.0 6 2 63 9 0.0 6 9 64 20 0.0 6 19 1 65 7 0.0 6 7 66 3 0.0 6 3 67 3 0.0 6 2 1 68 2 0.0 6 2 69 5 0.0 6 5 70 4 0.0 6 3 1 71 7 0.0 6 6 1 72 13 0.0 6 13 73 17 0.0 6 17 74 9 0.0 6 9 75 20 0.0 6 18 2 76 8 0.0 6 8 77 15 0.0 6 14 0 1 78 8 0.0 6 7 1 79 5 0.0 6 5 80 6 0.0 6 6 81 12 0.0 6 11 1 82 16 0.0 6 15 1 83 18 0.0 6 18 84 10 0.0 6 10 85 16 0.0 6 16 86 11 0.0 6 9 2 87 5 0.0 6 5 88 7 0.0 6 7 89 6 0.0 6 6 90 7 0.0 6 6 1 91 1 0.0 6 1 92 2 0.0 6 2 93 10 0.0 6 9 1 94 6 0.0 6 5 1 95 16 0.0 6 16 96 4 0.0 6 4 97 10 0.0 6 9 1 98 12 0.0 6 10 2 99 5 0.0 6 5 100 8 0.0 6 7 1 101 20 0.0 6 19 0 1 102 18 0.0 6 18 103 25 0.0 6 25 104 26 0.0 6 26 105 24 0.0 6 24 106 23 0.0 6 22 1 107 27 0.0 6 27 108 27 0.0 6 27 109 22 0.0 6 22 110 35 0.0 6 33 1 0 0 0 1 111 40 0.0 6 40 112 36 0.0 6 36 113 45 0.0 6 43 0 0 0 0 2 114 21 0.0 6 20 0 0 0 0 1 115 17 0.0 6 17 116 32 0.0 6 30 0 0 0 0 2 117 43 0.0 6 22 0 0 0 0 21 118 26 0.0 6 23 0 0 0 0 3 119 23 0.0 6 23 120 19 0.0 6 19 121 16 0.0 6 16 122 22 0.0 6 22 123 33 0.0 6 33 124 22 0.0 6 22 125 46 0.0 6 45 1 126 36 0.0 6 36 127 34 0.0 6 34 128 34 0.0 6 34 129 39 0.0 6 37 2 130 102 0.0 6 98 4 131 47 0.0 6 47 132 38 0.0 6 36 2 133 41 0.0 6 41 134 74 0.0 6 74 135 57 0.0 6 57 136 67 0.0 6 66 0 0 0 0 1 137 89 0.0 6 87 2 138 79 0.0 6 77 2 139 100 0.0 6 99 1 140 382 0.0 6 380 2 141 234 0.0 6 234 142 500 0.0 6 498 2 143 1168 0.0 6 1165 3 144 1580 0.0 6 1576 4 145 2204 0.0 6 2197 7 146 3442 0.0 6 3436 5 0 0 0 0 1 147 1135 0.0 6 1134 1 148 498 0.0 6 496 2 149 743 0.0 6 742 1 150 653 0.0 6 651 2 151 324 0.0 6 321 2 0 0 0 1 152 1931 0.0 6 1929 2 153 353 0.0 6 352 0 0 0 0 1 154 317 0.0 6 316 1 155 243 0.0 6 243 156 379 0.0 6 378 1 157 178 0.0 6 177 0 0 0 0 1 158 616 0.0 6 614 2 159 502 0.0 6 501 1 160 1410 0.0 6 1406 4 161 295 0.0 6 294 1 162 502 0.0 6 501 1 163 256 0.0 6 256 164 169 0.0 6 169 165 218 0.0 6 218 166 220 0.0 6 220 167 308 0.0 6 308 168 369 0.0 6 369 169 616 0.0 6 614 2 170 274 0.0 6 273 1 171 628 0.0 6 628 172 1137 0.0 6 1136 0 0 0 0 1 173 454 0.0 6 453 1 174 1700 0.0 6 1699 1 175 470 0.0 6 468 2 176 590 0.0 6 590 177 400 0.0 6 398 2 178 788 0.0 6 785 3 179 6992 0.0 6 6983 9 180 1045 0.0 6 1042 3 181 384 0.0 6 384 182 206 0.0 6 206 183 238 0.0 6 237 1 184 298 0.0 6 297 1 185 217 0.0 6 216 1 186 385 0.0 6 383 2 187 620 0.0 6 620 188 3010 0.0 6 3006 4 189 746 0.0 6 746 190 733 0.0 6 733 191 164 0.0 6 164 192 193 0.0 6 192 1 193 96 0.0 6 96 194 103 0.0 6 103 195 203 0.0 6 202 1 196 333 0.0 6 332 1 197 487 0.0 6 487 198 517 0.0 6 516 1 199 480 0.0 6 480 200 471 0.0 6 471 201 1136 0.0 6 1135 1 202 4002 0.0 6 3999 3 203 332 0.0 6 330 2 204 78 0.0 6 78 205 76 0.0 6 76 206 75 0.0 6 75 207 91 0.0 6 90 1 208 132 0.0 6 132 209 97 0.0 6 97 210 84 0.0 6 84 211 138 0.0 6 138 212 104 0.0 6 104 213 131 0.0 6 131 214 154 0.0 6 153 1 215 191 0.0 6 190 1 216 173 0.0 6 173 217 290 0.0 6 289 1 218 290 0.0 6 290 219 338 0.0 6 338 220 175 0.0 6 175 221 224 0.0 6 224 222 545 0.0 6 545 223 270 0.0 6 270 224 13 0.0 6 13 225 12 0.0 6 12 226 2 0.0 6 2 227 8 0.0 6 8 228 9 0.0 6 9 229 16 0.0 6 16 230 6 0.0 6 6 231 12 0.0 6 12 232 8 0.0 6 8 233 20 0.0 6 20 234 19 0.0 6 19 235 16 0.0 6 16 236 13 0.0 6 13 237 18 0.0 6 18 238 13 0.0 6 13 239 29 0.0 6 29 240 24 0.0 6 24 241 40 0.0 6 40 242 25 0.0 6 25 243 1 0.0 6 1 244 1 0.0 6 1 246 2 0.0 6 2 247 1 0.0 6 1 249 1 0.0 6 1 250 1 0.0 6 1 251 2 0.0 6 2 252 1 0.0 6 1 253 2 0.0 6 2 254 1 0.0 6 1 255 2 0.0 6 2 256 2 0.0 6 2 257 2 0.0 6 2 258 1 0.0 6 1 259 1 0.0 6 1 262 1 0.0 6 1 263 1 0.0 6 1 265 2 0.0 6 2 268 2 0.0 6 2 269 1 0.0 6 1 270 2 0.0 6 2 271 3 0.0 6 3 273 1 0.0 6 1 274 1 0.0 6 1 275 1 0.0 6 1 276 2 0.0 6 2 277 1 0.0 6 1 278 3 0.0 6 3 279 21 0.0 6 21 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2416 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 2 0.0 6 1 0 0 1 62 2 0.0 6 1 1 63 214 0.0 6 201 13 64 3 0.0 6 2 1 70 1 0.0 6 1 71 1 0.0 6 1 72 7 0.0 6 7 73 6 0.0 6 6 74 20 0.0 6 20 75 62 0.0 6 62 76 76 0.0 6 75 1 77 119 0.0 6 119 78 146 0.0 6 145 1 79 48 0.0 6 48 80 23 0.0 6 23 81 32 0.0 6 32 82 28 0.0 6 28 83 27 0.0 6 27 84 77 0.0 6 77 85 10 0.0 6 10 86 10 0.0 6 10 87 9 0.0 6 9 88 9 0.0 6 9 89 3 0.0 6 3 90 22 0.0 6 21 1 91 32 0.0 6 32 92 63 0.0 6 63 93 13 0.0 6 13 94 15 0.0 6 15 95 2 0.0 6 2 96 2 0.0 6 2 97 8 0.0 6 8 98 2 0.0 6 2 99 5 0.0 6 4 1 100 10 0.0 6 10 101 10 0.0 6 10 102 10 0.0 6 10 103 29 0.0 6 29 104 42 0.0 6 42 105 22 0.0 6 22 106 74 0.0 6 74 107 9 0.0 6 9 108 9 0.0 6 9 109 7 0.0 6 7 110 105 0.0 6 105 111 286 0.0 6 286 112 14 0.0 6 14 113 6 0.0 6 6 114 4 0.0 6 4 115 7 0.0 6 7 116 5 0.0 6 5 117 3 0.0 6 3 118 5 0.0 6 5 119 40 0.0 6 40 120 116 0.0 6 116 121 25 0.0 6 25 122 25 0.0 6 25 123 3 0.0 6 3 124 4 0.0 6 4 125 1 0.0 6 1 126 1 0.0 6 1 127 2 0.0 6 2 128 3 0.0 6 2 1 129 1 0.0 6 0 1 131 1 0.0 6 1 132 11 0.0 6 10 1 133 69 0.0 6 69 134 207 0.0 6 207 135 7 0.0 6 7 136 1 0.0 6 1 137 1 0.0 6 1 139 4 0.0 6 4 140 1 0.0 6 1 142 2 0.0 6 2 143 1 0.0 6 1 144 1 0.0 6 1 145 3 0.0 6 3 146 1 0.0 6 1 148 1 0.0 6 1 149 1 0.0 6 1 150 5 0.0 6 5 151 8 0.0 6 8 152 7 0.0 6 7 153 16 0.0 6 16 154 52 0.0 6 52 155 20 0.0 6 20 156 2 0.0 6 2 160 3 0.0 6 3 165 1 0.0 6 1 170 1 0.0 6 1 173 6 0.0 6 6 178 1 0.0 6 0 0 0 0 0 0 1 188 1 0.0 6 1 200 1 0.0 6 1 203 1 0.0 6 1 206 1 0.0 6 1 208 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 57625 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 8 1 0.9 2 1 11 3 0.0 3 3 12 2 0.0 3 2 13 3 0.0 3 2 1 14 4 0.0 4 4 15 1 0.0 4 1 16 4 0.0 4 3 0 1 17 4 0.0 5 4 18 1 0.0 5 1 19 10 0.0 5 8 2 20 34 0.0 6 32 2 21 18 0.0 6 15 3 22 25 0.0 6 25 23 13 0.0 6 13 24 8 0.0 6 8 25 10 0.0 6 9 1 26 7 0.0 6 7 27 7 0.0 6 6 1 28 10 0.0 6 10 29 8 0.0 6 8 30 11 0.0 6 11 31 2 0.0 6 2 32 7 0.0 6 6 1 33 4 0.0 6 4 34 4 0.0 6 3 0 0 0 0 1 35 4 0.0 6 3 1 36 9 0.0 6 6 1 2 37 2 0.0 6 2 38 6 0.0 6 4 2 39 5 0.0 6 4 1 40 8 0.0 6 8 41 2 0.0 6 2 42 10 0.0 6 9 0 0 0 0 1 43 7 0.0 6 7 44 7 0.0 6 7 45 9 0.0 6 9 46 17 0.0 6 17 47 10 0.0 6 9 1 48 14 0.0 6 13 1 49 10 0.0 6 9 1 50 4 0.0 6 4 51 10 0.0 6 10 52 9 0.0 6 9 53 7 0.0 6 7 54 14 0.0 6 14 55 13 0.0 6 12 0 1 56 14 0.0 6 13 1 57 14 0.0 6 12 2 58 13 0.0 6 12 0 0 1 59 9 0.0 6 8 1 60 9 0.0 6 8 1 61 11 0.0 6 11 62 10 0.0 6 10 63 15 0.0 6 13 2 64 14 0.0 6 13 1 65 23 0.0 6 23 66 22 0.0 6 22 67 16 0.0 6 16 68 19 0.0 6 18 1 69 34 0.0 6 33 0 1 70 107 0.0 6 105 2 71 42 0.0 6 37 4 0 0 0 1 72 40 0.0 6 39 1 73 21 0.0 6 21 74 34 0.0 6 31 3 75 16 0.0 6 15 1 76 18 0.0 6 16 2 77 27 0.0 6 23 3 1 78 27 0.0 6 26 0 1 79 21 0.0 6 21 80 21 0.0 6 21 81 27 0.0 6 27 82 35 0.0 6 35 83 44 0.0 6 43 1 84 24 0.0 6 23 1 85 41 0.0 6 39 1 1 86 61 0.0 6 59 2 87 36 0.0 6 35 1 88 24 0.0 6 24 89 25 0.0 6 24 1 90 28 0.0 6 28 91 34 0.0 6 34 92 37 0.0 6 37 93 27 0.0 6 27 94 46 0.0 6 46 95 37 0.0 6 35 2 96 69 0.0 6 68 1 97 33 0.0 6 32 1 98 47 0.0 6 47 99 44 0.0 6 43 1 100 42 0.0 6 41 1 101 45 0.0 6 43 2 102 41 0.0 6 41 103 82 0.0 6 82 104 63 0.0 6 63 105 52 0.0 6 52 106 66 0.0 6 66 107 108 0.0 6 108 108 62 0.0 6 62 109 77 0.0 6 77 110 62 0.0 6 61 1 111 77 0.0 6 77 112 86 0.0 6 86 113 105 0.0 6 104 0 1 114 118 0.0 6 118 115 113 0.0 6 112 1 116 92 0.0 6 92 117 87 0.0 6 87 118 123 0.0 6 122 1 119 128 0.0 6 128 120 160 0.0 6 160 121 161 0.0 6 161 122 128 0.0 6 128 123 131 0.0 6 130 1 124 129 0.0 6 129 125 187 0.0 6 186 1 126 189 0.0 6 189 127 185 0.0 6 183 2 128 275 0.0 6 274 1 129 251 0.0 6 251 130 236 0.0 6 232 4 131 287 0.0 6 280 7 132 207 0.0 6 204 3 133 195 0.0 6 195 134 260 0.0 6 260 135 316 0.0 6 316 136 321 0.0 6 320 1 137 400 0.0 6 399 1 138 358 0.0 6 355 2 0 0 1 139 464 0.0 6 463 1 140 506 0.0 6 505 1 141 511 0.0 6 510 1 142 760 0.0 6 759 1 143 1389 0.0 6 1386 3 144 1729 0.0 6 1724 5 145 2350 0.0 6 2341 9 146 2536 0.0 6 2533 2 0 0 0 0 1 147 1315 0.0 6 1313 2 148 989 0.0 6 986 3 149 933 0.0 6 932 1 150 943 0.0 6 943 151 925 0.0 6 923 1 0 0 1 152 1738 0.0 6 1735 3 153 1157 0.0 6 1155 2 154 891 0.0 6 891 155 948 0.0 6 947 1 156 1008 0.0 6 1006 2 157 828 0.0 6 826 2 158 1034 0.0 6 1032 1 0 0 0 0 1 159 961 0.0 6 958 2 0 1 160 1218 0.0 6 1217 1 161 701 0.0 6 700 1 162 1261 0.0 6 1258 2 1 163 638 0.0 6 637 0 1 164 682 0.0 6 682 165 472 0.0 6 471 1 166 521 0.0 6 521 167 549 0.0 6 546 3 168 822 0.0 6 822 169 804 0.0 6 803 1 170 641 0.0 6 639 2 171 1242 0.0 6 1241 1 172 1014 0.0 6 1014 173 747 0.0 6 747 174 1028 0.0 6 1028 175 800 0.0 6 799 0 0 0 0 1 176 1131 0.0 6 1128 2 0 0 1 177 1027 0.0 6 1027 178 1101 0.0 6 1098 2 1 179 1849 0.0 6 1848 1 180 329 0.0 6 327 1 0 0 0 1 181 407 0.0 6 407 182 411 0.0 6 410 0 0 0 0 0 1 183 359 0.0 6 359 184 256 0.0 6 256 185 599 0.0 6 599 186 476 0.0 6 476 187 620 0.0 6 620 188 885 0.0 6 884 1 189 241 0.0 6 241 190 314 0.0 6 314 191 165 0.0 6 165 192 164 0.0 6 164 193 144 0.0 6 144 194 104 0.0 6 104 195 176 0.0 6 176 196 161 0.0 6 160 1 197 142 0.0 6 141 1 198 110 0.0 6 110 199 302 0.0 6 302 200 229 0.0 6 229 201 266 0.0 6 266 202 399 0.0 6 399 203 51 0.0 6 51 204 47 0.0 6 47 205 58 0.0 6 57 1 206 32 0.0 6 32 207 29 0.0 6 29 208 13 0.0 6 13 209 10 0.0 6 10 210 7 0.0 6 7 211 11 0.0 6 11 212 9 0.0 6 9 213 4 0.0 6 4 214 6 0.0 6 6 215 5 0.0 6 5 216 14 0.0 6 14 217 22 0.0 6 22 218 33 0.0 6 33 219 34 0.0 6 34 220 24 0.0 6 24 221 22 0.0 6 22 222 31 0.0 6 31 223 23 0.0 6 23 224 4 0.0 6 4 227 2 0.0 6 2 232 2 0.0 6 2 233 1 0.0 6 1 235 1 0.0 6 1 237 1 0.0 6 1 238 1 0.0 6 1 243 1 0.0 6 1 251 1 0.0 6 1 280 1 0.0 6 1 281 1 0.0 6 0 0 1 282 3 0.0 6 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2693 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 29 0.0 6 0 29 58 2 0.0 6 0 2 59 74 0.0 6 69 5 60 1 0.0 6 1 62 1 0.0 6 0 0 0 1 63 5 0.0 6 4 0 0 0 1 68 2 0.0 6 2 69 21 0.0 6 21 70 7 0.0 6 7 71 19 0.0 6 18 1 72 56 0.0 6 55 1 73 91 0.0 6 91 74 121 0.0 6 119 1 0 1 75 203 0.0 6 203 76 44 0.0 6 43 0 0 0 1 77 21 0.0 6 20 0 0 0 1 78 45 0.0 6 44 0 0 1 79 46 0.0 6 44 0 0 0 2 80 25 0.0 6 25 81 111 0.0 6 111 82 10 0.0 6 10 83 9 0.0 6 9 84 9 0.0 6 8 0 0 0 1 85 15 0.0 6 15 86 11 0.0 6 11 87 29 0.0 6 29 88 34 0.0 6 34 89 76 0.0 6 76 90 11 0.0 6 10 1 91 13 0.0 6 13 92 7 0.0 6 7 93 2 0.0 6 2 94 6 0.0 6 6 95 5 0.0 6 5 96 3 0.0 6 3 97 10 0.0 6 10 98 13 0.0 6 13 99 8 0.0 6 8 100 33 0.0 6 32 1 101 54 0.0 6 53 1 102 15 0.0 6 15 103 78 0.0 6 78 104 4 0.0 6 4 105 7 0.0 6 7 106 8 0.0 6 8 107 61 0.0 6 61 108 407 0.0 6 407 109 4 0.0 6 4 110 14 0.0 6 13 0 0 1 111 5 0.0 6 3 0 0 1 1 112 4 0.0 6 2 0 0 0 2 113 3 0.0 6 3 114 2 0.0 6 1 1 115 9 0.0 6 9 116 30 0.0 6 30 117 151 0.0 6 151 118 28 0.0 6 28 119 23 0.0 6 23 120 3 0.0 6 3 121 1 0.0 6 1 122 1 0.0 6 1 123 1 0.0 6 1 124 4 0.0 6 4 125 9 0.0 6 9 126 6 0.0 6 6 127 1 0.0 6 1 128 3 0.0 6 3 129 4 0.0 6 4 130 36 0.0 6 36 131 324 0.0 6 324 132 6 0.0 6 5 1 133 1 0.0 6 1 134 1 0.0 6 1 138 6 0.0 6 6 139 1 0.0 6 1 140 7 0.0 6 7 142 5 0.0 6 5 145 1 0.0 6 1 146 1 0.0 6 1 147 4 0.0 6 4 148 17 0.0 6 17 149 2 0.0 6 1 1 150 11 0.0 6 11 151 60 0.0 6 60 152 24 0.0 6 24 153 2 0.0 6 2 154 1 0.0 6 1 163 2 0.0 6 2 169 4 0.0 6 4 170 4 0.0 6 4 171 1 0.0 6 1 175 1 0.0 6 1 185 1 0.0 6 1 201 1 0.0 6 1 203 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_18_21_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_18_248_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_18_21_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_18_248_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.49 s (20 µs/read; 2.96 M reads/minute). === Summary === Total read pairs processed: 73,365 Read 1 with adapter: 73,351 (100.0%) Read 2 with adapter: 73,317 (99.9%) == Read fate breakdown == Pairs that were too short: 24 (0.0%) Pairs discarded as untrimmed: 62 (0.1%) Pairs written (passing filters): 73,279 (99.9%) Total basepairs processed: 30,558,339 bp Read 1: 16,879,124 bp Read 2: 13,679,215 bp Total written (filtered): 4,859,644 bp (15.9%) Read 1: 2,439,516 bp Read 2: 2,420,128 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 71559 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1146.3 0 0 2 4 3 286.6 1 2 1 5 2 71.6 1 2 6 1 17.9 1 0 0 1 7 4 4.5 2 3 0 1 11 1 0.0 3 1 12 1 0.0 3 1 14 2 0.0 4 1 1 15 1 0.0 4 1 16 3 0.0 4 2 1 17 7 0.0 5 7 19 1 0.0 5 1 20 107 0.0 6 105 2 21 54 0.0 6 50 4 22 47 0.0 6 45 2 23 43 0.0 6 41 2 24 26 0.0 6 26 25 20 0.0 6 18 2 26 29 0.0 6 27 2 27 9 0.0 6 8 1 28 21 0.0 6 18 2 0 0 1 29 10 0.0 6 10 30 13 0.0 6 13 31 8 0.0 6 8 32 8 0.0 6 8 33 9 0.0 6 8 1 34 10 0.0 6 9 1 35 6 0.0 6 6 36 5 0.0 6 5 37 5 0.0 6 5 38 5 0.0 6 5 39 19 0.0 6 18 0 0 1 40 10 0.0 6 10 41 5 0.0 6 5 42 4 0.0 6 4 43 12 0.0 6 12 44 9 0.0 6 9 45 6 0.0 6 6 46 3 0.0 6 3 47 5 0.0 6 5 48 5 0.0 6 5 49 7 0.0 6 7 50 11 0.0 6 10 0 0 0 0 1 51 10 0.0 6 10 52 5 0.0 6 5 53 5 0.0 6 5 54 6 0.0 6 5 0 0 1 55 12 0.0 6 12 56 7 0.0 6 7 57 5 0.0 6 5 58 7 0.0 6 6 0 0 1 59 8 0.0 6 7 1 60 9 0.0 6 9 61 5 0.0 6 5 62 6 0.0 6 6 63 6 0.0 6 5 0 0 0 1 64 5 0.0 6 4 1 65 14 0.0 6 12 2 66 9 0.0 6 8 1 67 4 0.0 6 4 68 5 0.0 6 5 69 4 0.0 6 4 70 7 0.0 6 6 1 71 6 0.0 6 6 72 8 0.0 6 7 1 73 18 0.0 6 17 1 74 11 0.0 6 11 75 10 0.0 6 10 76 10 0.0 6 9 1 77 8 0.0 6 8 78 15 0.0 6 14 0 0 0 0 0 1 79 11 0.0 6 10 1 80 9 0.0 6 9 81 10 0.0 6 10 82 14 0.0 6 14 83 16 0.0 6 16 84 16 0.0 6 15 1 85 10 0.0 6 9 1 86 6 0.0 6 6 87 10 0.0 6 10 88 13 0.0 6 12 0 1 89 12 0.0 6 12 90 8 0.0 6 7 1 91 5 0.0 6 5 92 8 0.0 6 8 93 16 0.0 6 16 94 9 0.0 6 8 0 0 0 0 1 95 13 0.0 6 12 0 1 96 12 0.0 6 12 97 13 0.0 6 11 2 98 15 0.0 6 14 1 99 9 0.0 6 9 100 13 0.0 6 13 101 10 0.0 6 10 102 12 0.0 6 11 0 1 103 16 0.0 6 16 104 24 0.0 6 22 2 105 19 0.0 6 17 2 106 19 0.0 6 19 107 19 0.0 6 19 108 10 0.0 6 10 109 10 0.0 6 10 110 26 0.0 6 26 111 33 0.0 6 33 112 25 0.0 6 25 113 40 0.0 6 40 114 42 0.0 6 41 1 115 46 0.0 6 45 1 116 31 0.0 6 31 117 29 0.0 6 29 118 31 0.0 6 31 119 34 0.0 6 34 120 21 0.0 6 21 121 26 0.0 6 26 122 28 0.0 6 26 2 123 47 0.0 6 42 4 0 0 0 1 124 40 0.0 6 40 125 35 0.0 6 34 0 0 0 0 0 1 126 23 0.0 6 23 127 40 0.0 6 38 2 128 47 0.0 6 46 1 129 58 0.0 6 55 3 130 43 0.0 6 43 131 35 0.0 6 35 132 47 0.0 6 46 0 0 1 133 53 0.0 6 52 1 134 49 0.0 6 47 0 0 0 2 135 43 0.0 6 43 136 60 0.0 6 57 3 137 57 0.0 6 57 138 55 0.0 6 55 139 79 0.0 6 79 140 82 0.0 6 82 141 108 0.0 6 107 1 142 98 0.0 6 97 1 143 223 0.0 6 221 2 144 414 0.0 6 413 1 145 455 0.0 6 455 146 638 0.0 6 636 2 147 683 0.0 6 683 148 1066 0.0 6 1061 3 0 0 0 2 149 1002 0.0 6 1000 1 0 0 1 150 976 0.0 6 971 3 0 0 0 1 1 151 741 0.0 6 741 152 742 0.0 6 738 4 153 1040 0.0 6 1040 154 634 0.0 6 634 155 556 0.0 6 553 3 156 597 0.0 6 595 2 157 461 0.0 6 459 2 158 385 0.0 6 384 1 159 448 0.0 6 447 1 160 512 0.0 6 512 161 451 0.0 6 448 3 162 515 0.0 6 512 1 0 0 0 0 2 163 680 0.0 6 677 2 0 1 164 687 0.0 6 686 1 165 718 0.0 6 717 1 166 511 0.0 6 511 167 438 0.0 6 435 3 168 414 0.0 6 414 169 461 0.0 6 460 1 170 390 0.0 6 389 1 171 438 0.0 6 433 4 0 0 1 172 470 0.0 6 468 2 173 486 0.0 6 484 2 174 464 0.0 6 462 1 0 0 1 175 455 0.0 6 453 2 176 390 0.0 6 388 2 177 456 0.0 6 455 1 178 388 0.0 6 386 2 179 379 0.0 6 377 1 0 1 180 423 0.0 6 421 2 181 749 0.0 6 746 1 2 182 462 0.0 6 459 2 0 0 0 1 183 453 0.0 6 453 184 493 0.0 6 493 185 431 0.0 6 431 186 431 0.0 6 430 0 0 0 1 187 423 0.0 6 420 1 0 0 0 1 1 188 494 0.0 6 492 2 189 493 0.0 6 490 1 1 0 0 1 190 513 0.0 6 512 1 191 450 0.0 6 448 2 192 442 0.0 6 438 4 193 562 0.0 6 559 2 0 1 194 592 0.0 6 590 1 0 0 1 195 772 0.0 6 767 3 2 196 782 0.0 6 777 5 197 728 0.0 6 725 3 198 826 0.0 6 823 3 199 714 0.0 6 708 5 1 200 560 0.0 6 557 3 201 628 0.0 6 621 7 202 608 0.0 6 607 1 203 689 0.0 6 687 2 204 741 0.0 6 737 4 205 885 0.0 6 880 4 0 0 0 0 1 206 885 0.0 6 882 3 207 726 0.0 6 725 1 208 753 0.0 6 749 4 209 606 0.0 6 603 3 210 734 0.0 6 732 2 211 734 0.0 6 732 1 0 0 1 212 746 0.0 6 744 1 0 1 213 711 0.0 6 705 5 0 1 214 790 0.0 6 785 5 215 1021 0.0 6 1017 3 0 0 1 216 775 0.0 6 770 5 217 771 0.0 6 767 3 0 0 0 1 218 816 0.0 6 812 3 0 0 0 0 1 219 704 0.0 6 699 5 220 574 0.0 6 571 1 0 1 0 1 221 544 0.0 6 541 1 1 1 222 598 0.0 6 595 2 0 0 0 0 1 223 718 0.0 6 711 7 224 632 0.0 6 626 5 1 225 840 0.0 6 834 6 226 760 0.0 6 758 2 227 615 0.0 6 613 2 228 644 0.0 6 643 0 1 229 624 0.0 6 622 2 230 647 0.0 6 646 0 0 0 0 0 1 231 610 0.0 6 608 1 0 0 0 0 1 232 617 0.0 6 615 2 233 537 0.0 6 535 1 1 234 551 0.0 6 548 3 235 415 0.0 6 413 2 236 466 0.0 6 465 1 237 453 0.0 6 452 1 238 507 0.0 6 505 2 239 427 0.0 6 425 2 240 435 0.0 6 435 241 464 0.0 6 462 2 242 363 0.0 6 361 2 243 377 0.0 6 373 3 0 1 244 336 0.0 6 334 2 245 337 0.0 6 336 1 246 284 0.0 6 284 247 220 0.0 6 219 1 248 243 0.0 6 243 249 278 0.0 6 275 3 250 213 0.0 6 211 2 251 215 0.0 6 215 252 223 0.0 6 221 2 253 216 0.0 6 213 3 254 194 0.0 6 193 1 255 186 0.0 6 186 256 174 0.0 6 172 2 257 170 0.0 6 169 1 258 199 0.0 6 197 1 1 259 232 0.0 6 230 2 260 188 0.0 6 187 1 261 118 0.0 6 117 1 262 146 0.0 6 145 1 263 148 0.0 6 145 3 264 144 0.0 6 143 1 265 137 0.0 6 136 1 266 119 0.0 6 118 1 267 129 0.0 6 129 268 121 0.0 6 120 1 269 138 0.0 6 136 1 1 270 100 0.0 6 99 1 271 105 0.0 6 100 5 272 108 0.0 6 107 1 273 106 0.0 6 102 4 274 146 0.0 6 142 3 0 0 0 0 1 275 170 0.0 6 167 3 276 203 0.0 6 202 1 277 285 0.0 6 283 2 278 880 0.0 6 876 3 1 279 1352 0.0 6 1342 7 2 0 1 280 10 0.0 6 10 281 3 0.0 6 3 282 1 0.0 6 1 283 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1792 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 33 0.0 6 30 3 69 1 0.0 6 1 72 13 0.0 6 13 73 13 0.0 6 13 74 13 0.0 6 13 75 20 0.0 6 20 76 33 0.0 6 33 77 37 0.0 6 37 78 26 0.0 6 26 79 34 0.0 6 34 80 21 0.0 6 21 81 26 0.0 6 26 82 30 0.0 6 30 83 15 0.0 6 15 84 21 0.0 6 21 85 17 0.0 6 17 86 8 0.0 6 7 1 87 4 0.0 6 4 88 10 0.0 6 10 89 13 0.0 6 13 90 11 0.0 6 11 91 16 0.0 6 16 92 15 0.0 6 15 93 23 0.0 6 23 94 14 0.0 6 14 95 13 0.0 6 13 96 11 0.0 6 10 1 97 8 0.0 6 8 98 12 0.0 6 12 99 7 0.0 6 7 100 13 0.0 6 13 101 10 0.0 6 10 102 13 0.0 6 13 103 12 0.0 6 12 104 8 0.0 6 8 105 12 0.0 6 12 106 14 0.0 6 14 107 8 0.0 6 7 1 108 7 0.0 6 7 109 17 0.0 6 17 110 18 0.0 6 18 111 5 0.0 6 5 112 17 0.0 6 17 113 12 0.0 6 12 114 9 0.0 6 9 115 9 0.0 6 9 116 8 0.0 6 8 117 12 0.0 6 12 118 13 0.0 6 13 119 6 0.0 6 6 120 4 0.0 6 4 121 9 0.0 6 9 122 8 0.0 6 8 123 8 0.0 6 8 124 11 0.0 6 11 125 12 0.0 6 12 126 5 0.0 6 5 127 5 0.0 6 5 128 11 0.0 6 11 129 11 0.0 6 11 130 9 0.0 6 9 131 10 0.0 6 10 132 11 0.0 6 11 133 8 0.0 6 8 134 10 0.0 6 10 135 9 0.0 6 9 136 11 0.0 6 11 137 13 0.0 6 13 138 7 0.0 6 7 139 11 0.0 6 11 140 8 0.0 6 8 141 11 0.0 6 11 142 3 0.0 6 3 143 11 0.0 6 11 144 10 0.0 6 10 145 9 0.0 6 9 146 8 0.0 6 8 147 14 0.0 6 14 148 17 0.0 6 17 149 7 0.0 6 7 150 10 0.0 6 10 151 8 0.0 6 8 152 13 0.0 6 13 153 7 0.0 6 7 154 11 0.0 6 11 155 9 0.0 6 9 156 11 0.0 6 11 157 10 0.0 6 10 158 8 0.0 6 8 159 14 0.0 6 14 160 13 0.0 6 13 161 10 0.0 6 10 162 14 0.0 6 14 163 14 0.0 6 13 1 164 11 0.0 6 11 165 7 0.0 6 6 1 166 19 0.0 6 19 167 34 0.0 6 34 168 12 0.0 6 12 169 17 0.0 6 17 170 15 0.0 6 15 171 6 0.0 6 6 172 4 0.0 6 4 173 10 0.0 6 10 174 14 0.0 6 14 175 7 0.0 6 7 176 5 0.0 6 5 177 4 0.0 6 4 178 14 0.0 6 14 179 10 0.0 6 10 180 3 0.0 6 3 181 7 0.0 6 7 182 10 0.0 6 10 183 5 0.0 6 5 184 9 0.0 6 9 185 7 0.0 6 7 186 12 0.0 6 12 187 12 0.0 6 12 188 16 0.0 6 16 189 10 0.0 6 10 190 5 0.0 6 5 191 5 0.0 6 5 192 7 0.0 6 7 193 5 0.0 6 5 194 10 0.0 6 10 195 5 0.0 6 5 196 8 0.0 6 8 197 7 0.0 6 7 198 5 0.0 6 5 199 6 0.0 6 6 200 8 0.0 6 8 201 2 0.0 6 2 202 7 0.0 6 7 203 18 0.0 6 18 204 6 0.0 6 6 205 6 0.0 6 6 206 5 0.0 6 5 207 5 0.0 6 5 208 2 0.0 6 2 209 5 0.0 6 5 210 12 0.0 6 12 211 5 0.0 6 5 212 14 0.0 6 13 1 213 7 0.0 6 7 214 5 0.0 6 5 215 8 0.0 6 8 216 4 0.0 6 4 217 5 0.0 6 5 218 3 0.0 6 3 219 1 0.0 6 1 220 3 0.0 6 3 221 6 0.0 6 6 222 5 0.0 6 5 223 7 0.0 6 7 224 3 0.0 6 3 225 1 0.0 6 1 226 4 0.0 6 4 227 2 0.0 6 2 228 5 0.0 6 5 229 5 0.0 6 5 230 4 0.0 6 4 231 6 0.0 6 6 232 2 0.0 6 2 233 4 0.0 6 4 234 3 0.0 6 3 235 2 0.0 6 2 236 5 0.0 6 5 237 1 0.0 6 1 238 1 0.0 6 1 239 1 0.0 6 1 240 3 0.0 6 3 241 3 0.0 6 3 242 3 0.0 6 3 243 2 0.0 6 2 244 1 0.0 6 1 245 2 0.0 6 2 246 3 0.0 6 3 247 4 0.0 6 4 248 6 0.0 6 6 249 4 0.0 6 4 251 1 0.0 6 1 252 4 0.0 6 4 253 6 0.0 6 6 254 1 0.0 6 1 255 1 0.0 6 1 256 2 0.0 6 2 257 1 0.0 6 1 258 1 0.0 6 1 261 4 0.0 6 4 262 1 0.0 6 1 265 3 0.0 6 3 266 1 0.0 6 1 267 2 0.0 6 2 268 2 0.0 6 2 269 2 0.0 6 2 270 1 0.0 6 1 272 1 0.0 6 1 274 1 0.0 6 1 276 1 0.0 6 1 277 1 0.0 6 1 278 1 0.0 6 1 279 1 0.0 6 1 280 1 0.0 6 1 281 1 0.0 6 1 282 2 0.0 6 2 283 1 0.0 6 1 289 1 0.0 6 1 294 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 71311 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1146.3 0 0 2 7 1 4.5 2 0 0 1 9 1 0.3 2 1 10 3 0.1 3 3 11 2 0.0 3 2 12 2 0.0 3 1 1 13 10 0.0 3 7 3 14 7 0.0 4 7 15 4 0.0 4 3 1 16 13 0.0 4 12 1 17 23 0.0 5 20 3 18 18 0.0 5 16 2 19 15 0.0 5 10 5 20 103 0.0 6 100 3 21 70 0.0 6 68 2 22 49 0.0 6 48 1 23 38 0.0 6 36 2 24 18 0.0 6 18 25 31 0.0 6 29 2 26 11 0.0 6 10 1 27 15 0.0 6 13 2 28 20 0.0 6 19 1 29 21 0.0 6 19 2 30 17 0.0 6 16 1 31 16 0.0 6 15 1 32 13 0.0 6 13 33 14 0.0 6 14 34 13 0.0 6 13 35 14 0.0 6 12 2 36 19 0.0 6 18 0 1 37 14 0.0 6 12 0 2 38 14 0.0 6 13 1 39 19 0.0 6 17 1 1 40 14 0.0 6 11 2 1 41 11 0.0 6 9 2 42 12 0.0 6 9 2 1 43 19 0.0 6 15 3 0 0 0 1 44 17 0.0 6 15 2 45 18 0.0 6 17 1 46 18 0.0 6 16 2 47 14 0.0 6 14 48 18 0.0 6 15 3 49 29 0.0 6 24 4 1 50 11 0.0 6 10 0 0 0 1 51 17 0.0 6 17 52 21 0.0 6 19 2 53 20 0.0 6 19 0 0 0 0 0 1 54 21 0.0 6 19 2 55 25 0.0 6 24 1 56 27 0.0 6 22 5 57 27 0.0 6 24 3 58 26 0.0 6 22 4 59 23 0.0 6 23 60 28 0.0 6 26 1 1 61 25 0.0 6 24 1 62 28 0.0 6 26 2 63 34 0.0 6 33 1 64 38 0.0 6 36 1 0 0 1 65 31 0.0 6 30 1 66 47 0.0 6 42 5 67 137 0.0 6 128 7 1 0 1 68 67 0.0 6 66 1 69 84 0.0 6 80 4 70 40 0.0 6 37 2 0 1 71 63 0.0 6 59 3 1 72 38 0.0 6 34 3 0 0 1 73 34 0.0 6 33 0 0 0 1 74 50 0.0 6 44 6 75 42 0.0 6 39 3 76 39 0.0 6 38 1 77 50 0.0 6 50 78 38 0.0 6 37 1 79 52 0.0 6 49 2 1 80 41 0.0 6 38 2 1 81 62 0.0 6 60 2 82 59 0.0 6 56 2 0 1 83 72 0.0 6 66 6 84 44 0.0 6 44 85 50 0.0 6 46 3 0 0 0 0 1 86 42 0.0 6 40 1 0 0 0 1 87 53 0.0 6 49 3 1 88 53 0.0 6 52 1 89 55 0.0 6 55 90 65 0.0 6 63 1 0 0 1 91 64 0.0 6 62 1 1 92 70 0.0 6 68 2 93 71 0.0 6 70 1 94 84 0.0 6 78 5 0 0 0 1 95 78 0.0 6 75 1 1 0 0 0 1 96 92 0.0 6 90 2 97 89 0.0 6 87 1 0 0 0 0 1 98 88 0.0 6 80 8 99 79 0.0 6 74 4 0 0 0 1 100 87 0.0 6 86 1 101 100 0.0 6 97 3 102 101 0.0 6 98 2 0 0 0 0 1 103 115 0.0 6 109 4 1 0 1 104 205 0.0 6 201 3 0 0 0 1 105 115 0.0 6 112 3 106 110 0.0 6 103 7 107 126 0.0 6 123 2 0 0 1 108 102 0.0 6 99 2 0 0 0 0 1 109 117 0.0 6 114 3 110 134 0.0 6 130 2 0 0 0 1 1 111 120 0.0 6 117 3 112 148 0.0 6 143 5 113 132 0.0 6 128 4 114 135 0.0 6 134 1 115 186 0.0 6 179 5 0 0 2 116 195 0.0 6 193 1 0 0 0 0 1 117 179 0.0 6 175 3 1 118 215 0.0 6 209 3 0 1 1 0 1 119 194 0.0 6 190 4 120 187 0.0 6 184 2 0 1 121 199 0.0 6 192 6 0 0 1 122 201 0.0 6 199 2 123 215 0.0 6 210 5 124 233 0.0 6 228 4 0 0 0 1 125 309 0.0 6 305 4 126 289 0.0 6 284 5 127 314 0.0 6 308 6 128 345 0.0 6 338 6 0 0 1 129 370 0.0 6 363 6 0 0 1 130 412 0.0 6 405 6 0 0 0 0 1 131 466 0.0 6 458 7 0 0 1 132 400 0.0 6 394 6 133 483 0.0 6 477 3 0 2 0 1 134 510 0.0 6 500 6 2 0 1 0 1 135 490 0.0 6 485 4 0 0 1 136 439 0.0 6 433 5 1 137 529 0.0 6 520 5 2 1 0 1 138 513 0.0 6 504 5 2 1 1 139 569 0.0 6 563 5 1 140 710 0.0 6 700 8 0 0 1 1 141 984 0.0 6 972 8 0 2 0 2 142 1159 0.0 6 1147 9 3 143 987 0.0 6 976 10 0 0 1 144 1241 0.0 6 1225 11 0 1 1 0 3 145 1373 0.0 6 1360 9 2 0 0 0 2 146 1668 0.0 6 1650 15 1 0 1 0 1 147 1515 0.0 6 1507 6 0 0 0 1 1 148 1512 0.0 6 1497 11 2 0 1 0 1 149 1504 0.0 6 1489 8 2 2 1 1 1 150 2152 0.0 6 2123 24 3 0 2 151 1516 0.0 6 1495 18 0 0 2 1 152 1648 0.0 6 1624 17 1 1 4 1 153 1651 0.0 6 1628 20 0 1 0 1 1 154 1248 0.0 6 1228 15 2 1 1 1 155 1318 0.0 6 1296 20 0 0 0 1 1 156 1197 0.0 6 1187 9 0 0 0 0 1 157 1354 0.0 6 1342 11 0 0 1 158 1392 0.0 6 1375 16 1 159 1195 0.0 6 1179 14 0 1 0 1 160 1313 0.0 6 1299 12 1 0 1 161 1171 0.0 6 1164 5 0 1 0 0 1 162 1095 0.0 6 1083 10 1 1 163 926 0.0 6 919 4 0 2 0 0 1 164 937 0.0 6 925 10 0 1 1 165 891 0.0 6 885 4 1 0 1 166 1093 0.0 6 1085 7 0 0 0 0 1 167 892 0.0 6 884 6 1 1 168 1082 0.0 6 1077 5 169 964 0.0 6 958 6 170 925 0.0 6 916 8 1 171 992 0.0 6 986 5 1 172 971 0.0 6 961 7 1 1 0 0 1 173 934 0.0 6 926 7 0 0 0 0 1 174 874 0.0 6 864 7 0 1 1 1 175 852 0.0 6 843 6 1 1 0 0 1 176 902 0.0 6 895 6 0 0 0 0 1 177 735 0.0 6 730 5 178 742 0.0 6 737 5 179 571 0.0 6 568 3 180 535 0.0 6 530 5 181 512 0.0 6 509 3 182 511 0.0 6 507 3 0 0 1 183 687 0.0 6 686 1 184 901 0.0 6 893 7 0 0 1 185 766 0.0 6 758 7 1 186 567 0.0 6 563 4 187 540 0.0 6 536 4 188 473 0.0 6 470 3 189 448 0.0 6 444 2 0 0 0 0 2 190 416 0.0 6 415 0 0 1 191 438 0.0 6 434 4 192 327 0.0 6 326 1 193 280 0.0 6 279 1 194 302 0.0 6 298 4 195 252 0.0 6 248 4 196 349 0.0 6 344 4 0 0 1 197 274 0.0 6 271 3 198 235 0.0 6 232 3 199 219 0.0 6 215 4 200 198 0.0 6 197 1 201 179 0.0 6 178 1 202 191 0.0 6 191 203 138 0.0 6 137 1 204 139 0.0 6 137 2 205 119 0.0 6 116 3 206 119 0.0 6 118 1 207 112 0.0 6 110 2 208 113 0.0 6 112 1 209 101 0.0 6 100 1 210 58 0.0 6 58 211 65 0.0 6 65 212 82 0.0 6 81 1 213 85 0.0 6 83 2 214 99 0.0 6 93 6 215 64 0.0 6 63 0 1 216 64 0.0 6 63 0 0 0 1 217 63 0.0 6 62 1 218 47 0.0 6 47 219 36 0.0 6 36 220 33 0.0 6 33 221 49 0.0 6 49 222 37 0.0 6 37 223 33 0.0 6 32 1 224 23 0.0 6 21 2 225 23 0.0 6 23 226 16 0.0 6 16 227 10 0.0 6 10 228 17 0.0 6 17 229 24 0.0 6 24 230 9 0.0 6 9 231 10 0.0 6 10 232 15 0.0 6 13 2 233 9 0.0 6 9 234 4 0.0 6 4 235 1 0.0 6 1 236 3 0.0 6 3 238 1 0.0 6 1 239 2 0.0 6 2 240 4 0.0 6 4 241 7 0.0 6 7 242 5 0.0 6 5 243 5 0.0 6 5 245 2 0.0 6 2 246 4 0.0 6 4 247 3 0.0 6 3 248 1 0.0 6 1 251 1 0.0 6 1 252 1 0.0 6 1 277 1 0.0 6 1 278 8 0.0 6 8 279 77 0.0 6 76 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2006 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 52 4 0.0 6 4 53 12 0.0 6 12 57 17 0.0 6 17 60 1 0.0 6 1 61 2 0.0 6 1 0 0 0 1 63 2 0.0 6 2 66 3 0.0 6 3 68 21 0.0 6 21 70 22 0.0 6 21 1 71 4 0.0 6 4 72 8 0.0 6 8 73 15 0.0 6 15 74 18 0.0 6 18 75 20 0.0 6 20 76 26 0.0 6 25 1 77 160 0.0 6 158 2 78 47 0.0 6 47 79 32 0.0 6 32 80 31 0.0 6 31 81 40 0.0 6 39 0 0 0 1 82 50 0.0 6 50 83 18 0.0 6 18 84 16 0.0 6 15 1 85 27 0.0 6 27 86 17 0.0 6 17 87 8 0.0 6 8 88 25 0.0 6 25 89 18 0.0 6 18 90 19 0.0 6 19 91 17 0.0 6 17 92 43 0.0 6 43 93 27 0.0 6 27 94 46 0.0 6 46 95 14 0.0 6 14 96 10 0.0 6 10 97 8 0.0 6 8 98 8 0.0 6 8 99 6 0.0 6 6 100 9 0.0 6 9 101 14 0.0 6 13 1 102 21 0.0 6 21 103 12 0.0 6 11 1 104 15 0.0 6 15 105 9 0.0 6 8 0 0 1 106 14 0.0 6 14 107 5 0.0 6 5 108 7 0.0 6 7 109 11 0.0 6 11 110 23 0.0 6 23 111 11 0.0 6 11 112 48 0.0 6 47 1 113 38 0.0 6 38 114 10 0.0 6 10 115 8 0.0 6 8 116 5 0.0 6 5 117 12 0.0 6 12 118 13 0.0 6 13 119 8 0.0 6 8 120 37 0.0 6 35 2 121 6 0.0 6 6 122 11 0.0 6 11 123 8 0.0 6 8 124 19 0.0 6 19 125 15 0.0 6 15 126 6 0.0 6 6 127 6 0.0 6 6 128 8 0.0 6 8 129 6 0.0 6 5 1 130 10 0.0 6 10 131 13 0.0 6 13 132 5 0.0 6 5 133 8 0.0 6 8 134 20 0.0 6 20 135 11 0.0 6 11 136 14 0.0 6 13 1 137 14 0.0 6 14 138 10 0.0 6 10 139 19 0.0 6 19 140 15 0.0 6 15 141 11 0.0 6 11 142 2 0.0 6 2 143 19 0.0 6 19 144 8 0.0 6 8 145 12 0.0 6 12 146 5 0.0 6 5 147 11 0.0 6 11 148 20 0.0 6 20 149 16 0.0 6 16 150 11 0.0 6 11 151 7 0.0 6 7 152 14 0.0 6 13 1 153 4 0.0 6 4 154 13 0.0 6 13 155 5 0.0 6 5 156 6 0.0 6 6 157 8 0.0 6 8 158 23 0.0 6 23 159 11 0.0 6 11 160 12 0.0 6 12 161 12 0.0 6 12 162 11 0.0 6 11 163 15 0.0 6 15 164 12 0.0 6 12 165 15 0.0 6 15 166 13 0.0 6 13 167 17 0.0 6 17 168 13 0.0 6 13 169 15 0.0 6 15 170 14 0.0 6 14 171 7 0.0 6 7 172 8 0.0 6 8 173 11 0.0 6 11 174 18 0.0 6 18 175 10 0.0 6 10 176 8 0.0 6 8 177 11 0.0 6 10 1 178 6 0.0 6 6 179 12 0.0 6 12 180 6 0.0 6 6 181 6 0.0 6 6 182 3 0.0 6 3 183 2 0.0 6 2 184 4 0.0 6 4 185 9 0.0 6 9 186 7 0.0 6 7 187 8 0.0 6 8 188 10 0.0 6 10 189 1 0.0 6 1 190 5 0.0 6 5 191 6 0.0 6 6 192 7 0.0 6 7 193 3 0.0 6 3 194 4 0.0 6 4 195 2 0.0 6 2 196 2 0.0 6 2 197 3 0.0 6 3 198 6 0.0 6 6 199 4 0.0 6 4 200 2 0.0 6 2 201 3 0.0 6 2 0 0 1 202 3 0.0 6 3 203 2 0.0 6 2 204 2 0.0 6 2 205 3 0.0 6 3 206 4 0.0 6 4 207 2 0.0 6 2 209 1 0.0 6 1 211 1 0.0 6 1 212 1 0.0 6 1 213 2 0.0 6 2 215 1 0.0 6 1 217 2 0.0 6 2 218 3 0.0 6 3 219 1 0.0 6 1 221 1 0.0 6 1 222 1 0.0 6 1 223 1 0.0 6 1 224 1 0.0 6 1 225 1 0.0 6 1 226 1 0.0 6 1 228 1 0.0 6 1 229 1 0.0 6 1 233 1 0.0 6 1 236 1 0.0 6 1 241 1 0.0 6 1 254 1 0.0 6 1 260 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_19_22_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_19_249_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_19_22_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_19_249_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.79 s (47 µs/read; 1.29 M reads/minute). === Summary === Total read pairs processed: 38,438 Read 1 with adapter: 38,436 (100.0%) Read 2 with adapter: 38,420 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 20 (0.1%) Pairs written (passing filters): 38,418 (99.9%) Total basepairs processed: 19,548,354 bp Read 1: 11,131,442 bp Read 2: 8,416,912 bp Total written (filtered): 2,381,620 bp (12.2%) Read 1: 1,229,286 bp Read 2: 1,152,334 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 38433 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 600.6 0 0 1 7 1 2.3 2 0 0 1 15 1 0.0 4 1 20 7 0.0 6 7 21 2 0.0 6 2 22 4 0.0 6 3 1 23 1 0.0 6 1 24 1 0.0 6 1 25 3 0.0 6 3 26 2 0.0 6 2 27 3 0.0 6 3 28 2 0.0 6 2 29 2 0.0 6 2 31 1 0.0 6 1 35 2 0.0 6 0 2 37 1 0.0 6 1 40 1 0.0 6 1 41 3 0.0 6 3 42 1 0.0 6 1 44 3 0.0 6 3 46 2 0.0 6 2 47 2 0.0 6 2 50 1 0.0 6 1 53 1 0.0 6 1 54 1 0.0 6 0 1 56 3 0.0 6 2 1 57 3 0.0 6 3 58 1 0.0 6 1 59 1 0.0 6 1 60 2 0.0 6 2 62 2 0.0 6 2 63 1 0.0 6 1 64 1 0.0 6 1 65 1 0.0 6 1 67 2 0.0 6 2 68 4 0.0 6 2 1 0 1 69 1 0.0 6 1 71 1 0.0 6 1 74 1 0.0 6 1 77 4 0.0 6 4 78 1 0.0 6 1 79 1 0.0 6 1 80 1 0.0 6 1 86 4 0.0 6 4 87 1 0.0 6 1 88 1 0.0 6 0 1 89 5 0.0 6 5 90 3 0.0 6 3 91 1 0.0 6 1 92 3 0.0 6 3 93 2 0.0 6 1 1 94 1 0.0 6 1 98 4 0.0 6 3 0 1 100 6 0.0 6 6 101 3 0.0 6 2 1 102 4 0.0 6 3 1 103 1 0.0 6 1 105 2 0.0 6 1 1 106 2 0.0 6 2 107 4 0.0 6 3 1 108 9 0.0 6 9 109 3 0.0 6 2 1 111 1 0.0 6 1 112 2 0.0 6 2 113 4 0.0 6 4 114 1 0.0 6 1 115 4 0.0 6 4 117 4 0.0 6 4 119 4 0.0 6 3 1 120 1 0.0 6 1 121 3 0.0 6 3 122 12 0.0 6 12 123 4 0.0 6 4 124 5 0.0 6 5 125 3 0.0 6 3 126 5 0.0 6 5 127 2 0.0 6 2 128 13 0.0 6 13 129 3 0.0 6 3 130 2 0.0 6 1 1 132 5 0.0 6 5 133 4 0.0 6 4 134 9 0.0 6 8 1 135 5 0.0 6 5 136 8 0.0 6 7 1 137 21 0.0 6 20 1 138 7 0.0 6 7 139 6 0.0 6 6 140 14 0.0 6 13 1 141 14 0.0 6 14 142 9 0.0 6 9 143 10 0.0 6 10 144 20 0.0 6 20 145 20 0.0 6 19 1 146 11 0.0 6 11 147 13 0.0 6 13 148 11 0.0 6 11 149 10 0.0 6 10 150 9 0.0 6 8 1 151 10 0.0 6 10 152 7 0.0 6 7 153 8 0.0 6 8 154 26 0.0 6 26 155 7 0.0 6 7 156 5 0.0 6 5 157 19 0.0 6 16 2 0 0 1 158 11 0.0 6 11 159 7 0.0 6 7 160 14 0.0 6 13 1 161 13 0.0 6 13 162 16 0.0 6 15 1 163 16 0.0 6 16 164 13 0.0 6 12 1 165 12 0.0 6 10 2 166 6 0.0 6 6 167 13 0.0 6 13 168 13 0.0 6 12 1 169 22 0.0 6 21 1 170 15 0.0 6 14 1 171 15 0.0 6 15 172 24 0.0 6 24 173 17 0.0 6 16 0 1 174 28 0.0 6 27 1 175 22 0.0 6 20 1 1 176 20 0.0 6 19 1 177 21 0.0 6 21 178 21 0.0 6 19 2 179 26 0.0 6 26 180 28 0.0 6 28 181 29 0.0 6 28 1 182 38 0.0 6 38 183 42 0.0 6 40 2 184 28 0.0 6 28 185 61 0.0 6 58 2 0 0 0 0 1 186 67 0.0 6 65 2 187 52 0.0 6 50 2 188 65 0.0 6 63 2 189 42 0.0 6 40 2 190 51 0.0 6 50 1 191 50 0.0 6 48 2 192 57 0.0 6 53 4 193 88 0.0 6 86 1 1 194 78 0.0 6 75 3 195 101 0.0 6 99 2 196 111 0.0 6 106 5 197 98 0.0 6 95 3 198 97 0.0 6 93 4 199 68 0.0 6 66 2 200 124 0.0 6 124 201 90 0.0 6 88 2 202 103 0.0 6 100 3 203 99 0.0 6 95 3 0 0 0 0 1 204 88 0.0 6 87 1 205 119 0.0 6 117 2 206 131 0.0 6 124 7 207 117 0.0 6 114 3 208 119 0.0 6 114 5 209 150 0.0 6 143 7 210 123 0.0 6 115 8 211 193 0.0 6 191 2 212 110 0.0 6 105 4 1 213 151 0.0 6 148 3 214 260 0.0 6 256 4 215 138 0.0 6 135 3 216 193 0.0 6 190 3 217 144 0.0 6 141 3 218 123 0.0 6 119 4 219 135 0.0 6 131 4 220 113 0.0 6 108 5 221 97 0.0 6 96 1 222 166 0.0 6 162 3 1 223 171 0.0 6 164 7 224 178 0.0 6 175 3 225 188 0.0 6 186 1 0 1 226 297 0.0 6 289 8 227 202 0.0 6 194 8 228 173 0.0 6 168 5 229 151 0.0 6 150 1 230 125 0.0 6 122 3 231 123 0.0 6 122 1 232 117 0.0 6 113 4 233 107 0.0 6 103 4 234 115 0.0 6 112 2 1 235 87 0.0 6 84 2 1 236 114 0.0 6 107 7 237 163 0.0 6 160 3 238 238 0.0 6 233 5 239 229 0.0 6 226 3 240 363 0.0 6 357 5 1 241 256 0.0 6 252 4 242 321 0.0 6 313 8 243 173 0.0 6 171 0 1 0 1 244 187 0.0 6 185 2 245 255 0.0 6 252 3 246 229 0.0 6 228 1 247 212 0.0 6 210 2 248 169 0.0 6 167 2 249 229 0.0 6 222 7 250 246 0.0 6 242 4 251 273 0.0 6 263 10 252 389 0.0 6 378 11 253 205 0.0 6 200 4 1 254 275 0.0 6 270 5 255 300 0.0 6 295 4 0 1 256 346 0.0 6 337 9 257 437 0.0 6 432 4 1 258 339 0.0 6 329 10 259 566 0.0 6 556 9 1 260 572 0.0 6 565 7 261 312 0.0 6 304 8 262 248 0.0 6 244 4 263 400 0.0 6 396 4 264 219 0.0 6 216 3 265 284 0.0 6 277 5 2 266 382 0.0 6 373 8 0 0 0 0 1 267 317 0.0 6 312 4 1 268 392 0.0 6 382 10 269 834 0.0 6 820 14 270 273 0.0 6 269 4 271 400 0.0 6 395 5 272 425 0.0 6 415 10 273 608 0.0 6 590 18 274 902 0.0 6 881 20 0 1 275 581 0.0 6 572 7 2 276 1050 0.0 6 1030 17 3 277 1523 0.0 6 1489 34 278 5694 0.0 6 5537 152 4 1 279 9210 0.0 6 9056 149 5 280 47 0.0 6 44 3 281 5 0.0 6 4 0 1 282 2 0.0 6 2 283 2 0.0 6 2 284 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 3 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 74 1 0.0 6 1 126 1 0.0 6 1 162 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 38419 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 11 1 0.0 3 1 12 6 0.0 3 6 13 9 0.0 3 7 2 14 3 0.0 4 3 15 1 0.0 4 0 1 16 7 0.0 4 5 2 17 4 0.0 5 3 1 18 9 0.0 5 7 2 19 3 0.0 5 3 20 21 0.0 6 20 1 21 16 0.0 6 16 22 11 0.0 6 11 23 13 0.0 6 13 24 8 0.0 6 8 25 9 0.0 6 8 1 26 6 0.0 6 6 27 3 0.0 6 3 29 6 0.0 6 6 30 7 0.0 6 7 31 8 0.0 6 8 32 10 0.0 6 10 33 2 0.0 6 2 34 7 0.0 6 7 35 5 0.0 6 5 36 5 0.0 6 5 37 8 0.0 6 8 38 4 0.0 6 3 0 1 39 3 0.0 6 3 40 6 0.0 6 6 41 5 0.0 6 5 42 3 0.0 6 3 43 8 0.0 6 8 44 8 0.0 6 8 45 4 0.0 6 4 46 19 0.0 6 18 1 47 14 0.0 6 11 3 48 4 0.0 6 3 1 49 9 0.0 6 8 0 0 1 50 12 0.0 6 9 3 51 6 0.0 6 6 52 16 0.0 6 14 1 0 0 1 53 7 0.0 6 7 54 11 0.0 6 10 1 55 15 0.0 6 15 56 10 0.0 6 10 57 9 0.0 6 8 1 58 4 0.0 6 4 59 6 0.0 6 6 60 3 0.0 6 3 61 2 0.0 6 2 62 12 0.0 6 12 63 3 0.0 6 2 1 64 7 0.0 6 6 1 65 3 0.0 6 2 1 66 11 0.0 6 9 2 67 14 0.0 6 13 0 1 68 10 0.0 6 8 2 69 46 0.0 6 44 2 70 6 0.0 6 5 1 71 31 0.0 6 30 1 72 14 0.0 6 13 1 73 19 0.0 6 19 74 10 0.0 6 7 2 0 0 0 1 75 3 0.0 6 3 76 12 0.0 6 12 77 5 0.0 6 5 78 21 0.0 6 19 2 79 9 0.0 6 9 80 7 0.0 6 7 81 6 0.0 6 5 1 82 13 0.0 6 11 2 83 18 0.0 6 17 1 84 25 0.0 6 24 1 85 24 0.0 6 23 1 86 22 0.0 6 20 2 87 18 0.0 6 16 1 0 0 1 88 10 0.0 6 8 2 89 22 0.0 6 21 1 90 22 0.0 6 20 2 91 22 0.0 6 21 0 1 92 10 0.0 6 9 0 1 93 21 0.0 6 18 3 94 50 0.0 6 48 2 95 12 0.0 6 11 1 96 19 0.0 6 18 1 97 24 0.0 6 24 98 17 0.0 6 16 1 99 13 0.0 6 12 1 100 29 0.0 6 27 2 101 23 0.0 6 23 102 51 0.0 6 48 2 1 103 38 0.0 6 36 2 104 49 0.0 6 47 2 105 32 0.0 6 28 3 1 106 25 0.0 6 24 1 107 38 0.0 6 35 2 1 108 24 0.0 6 23 0 0 1 109 33 0.0 6 31 2 110 29 0.0 6 27 1 1 111 41 0.0 6 38 3 112 30 0.0 6 27 2 1 113 20 0.0 6 16 4 114 33 0.0 6 33 115 40 0.0 6 34 5 1 116 37 0.0 6 37 117 20 0.0 6 19 1 118 34 0.0 6 33 1 119 49 0.0 6 45 4 120 52 0.0 6 51 1 121 32 0.0 6 29 3 122 38 0.0 6 38 123 37 0.0 6 34 2 1 124 48 0.0 6 45 3 125 33 0.0 6 29 4 126 33 0.0 6 30 3 127 35 0.0 6 34 1 128 25 0.0 6 25 129 27 0.0 6 26 1 130 27 0.0 6 27 131 54 0.0 6 53 1 132 81 0.0 6 80 1 133 44 0.0 6 39 4 0 1 134 79 0.0 6 74 5 135 148 0.0 6 145 3 136 84 0.0 6 80 3 1 137 104 0.0 6 102 2 138 50 0.0 6 48 2 139 103 0.0 6 97 6 140 117 0.0 6 112 4 1 141 107 0.0 6 105 2 142 165 0.0 6 159 6 143 124 0.0 6 119 5 144 169 0.0 6 164 5 145 107 0.0 6 103 4 146 89 0.0 6 84 5 147 54 0.0 6 52 2 148 106 0.0 6 103 2 1 149 186 0.0 6 181 5 150 220 0.0 6 213 6 1 151 126 0.0 6 121 5 152 340 0.0 6 331 7 2 153 156 0.0 6 153 2 1 154 204 0.0 6 199 5 155 158 0.0 6 155 3 156 151 0.0 6 143 8 157 234 0.0 6 226 8 158 262 0.0 6 256 6 159 129 0.0 6 125 3 1 160 241 0.0 6 232 7 0 0 2 161 137 0.0 6 134 3 162 152 0.0 6 146 6 163 213 0.0 6 206 7 164 183 0.0 6 176 7 165 236 0.0 6 227 7 0 0 2 166 254 0.0 6 247 7 167 175 0.0 6 171 4 168 311 0.0 6 300 11 169 112 0.0 6 106 6 170 204 0.0 6 197 7 171 304 0.0 6 299 5 172 122 0.0 6 120 2 173 213 0.0 6 210 2 1 174 278 0.0 6 268 8 1 1 175 555 0.0 6 540 14 0 1 176 311 0.0 6 298 13 177 414 0.0 6 400 14 178 306 0.0 6 298 8 179 353 0.0 6 341 10 2 180 378 0.0 6 369 9 181 396 0.0 6 387 9 182 159 0.0 6 148 9 1 1 183 404 0.0 6 399 4 0 0 1 184 78 0.0 6 71 7 185 170 0.0 6 162 8 186 787 0.0 6 774 12 1 187 700 0.0 6 679 21 188 740 0.0 6 718 22 189 627 0.0 6 615 11 0 1 190 1009 0.0 6 984 25 191 821 0.0 6 803 17 1 192 493 0.0 6 483 10 193 488 0.0 6 472 15 0 0 1 194 557 0.0 6 536 19 1 0 1 195 875 0.0 6 839 35 1 196 1275 0.0 6 1244 30 0 1 197 383 0.0 6 367 14 1 0 1 198 1377 0.0 6 1340 36 0 0 0 1 199 1307 0.0 6 1274 33 200 695 0.0 6 665 29 1 201 750 0.0 6 729 20 0 1 202 531 0.0 6 513 14 2 2 203 496 0.0 6 483 12 1 204 637 0.0 6 619 18 205 314 0.0 6 305 9 206 300 0.0 6 292 8 207 243 0.0 6 232 11 208 439 0.0 6 433 6 209 186 0.0 6 182 4 210 280 0.0 6 274 6 211 387 0.0 6 370 15 2 212 331 0.0 6 324 7 213 132 0.0 6 122 9 1 214 343 0.0 6 338 5 215 371 0.0 6 365 6 216 612 0.0 6 588 24 217 357 0.0 6 342 15 218 310 0.0 6 301 8 1 219 336 0.0 6 326 10 220 236 0.0 6 224 10 1 1 221 229 0.0 6 226 3 222 223 0.0 6 217 5 0 0 0 1 223 342 0.0 6 333 8 0 0 1 224 262 0.0 6 254 8 225 301 0.0 6 293 8 226 320 0.0 6 313 6 1 227 345 0.0 6 334 11 228 269 0.0 6 255 12 2 229 408 0.0 6 402 6 230 698 0.0 6 687 11 231 252 0.0 6 242 10 232 420 0.0 6 405 13 0 1 0 1 233 195 0.0 6 193 2 234 259 0.0 6 253 6 235 107 0.0 6 105 2 236 122 0.0 6 120 2 237 108 0.0 6 105 3 238 58 0.0 6 57 1 239 25 0.0 6 25 240 74 0.0 6 70 4 241 83 0.0 6 78 5 242 95 0.0 6 95 243 112 0.0 6 108 3 0 0 0 0 1 244 79 0.0 6 78 1 245 89 0.0 6 87 1 1 246 81 0.0 6 79 2 247 57 0.0 6 54 3 248 41 0.0 6 39 1 1 249 69 0.0 6 69 250 38 0.0 6 37 1 251 89 0.0 6 85 4 252 41 0.0 6 39 1 0 0 1 253 58 0.0 6 56 1 0 0 1 254 14 0.0 6 14 255 9 0.0 6 9 256 9 0.0 6 9 260 1 0.0 6 1 262 1 0.0 6 1 279 2 0.0 6 2 280 10 0.0 6 10 281 40 0.0 6 38 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 177 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_20_23_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_20_250_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_20_23_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_20_250_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.80 s (39 µs/read; 1.52 M reads/minute). === Summary === Total read pairs processed: 45,643 Read 1 with adapter: 45,639 (100.0%) Read 2 with adapter: 45,617 (99.9%) == Read fate breakdown == Pairs that were too short: 4 (0.0%) Pairs discarded as untrimmed: 29 (0.1%) Pairs written (passing filters): 45,610 (99.9%) Total basepairs processed: 22,275,843 bp Read 1: 12,553,335 bp Read 2: 9,722,508 bp Total written (filtered): 2,935,222 bp (13.2%) Read 1: 1,470,554 bp Read 2: 1,464,668 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 45430 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 713.2 0 0 1 4 2 178.3 1 1 1 5 1 44.6 1 1 7 1 2.8 2 1 14 1 0.0 4 1 17 3 0.0 5 3 20 16 0.0 6 16 21 3 0.0 6 3 22 6 0.0 6 6 23 8 0.0 6 8 24 6 0.0 6 6 25 6 0.0 6 5 1 26 8 0.0 6 7 0 1 27 6 0.0 6 5 1 28 2 0.0 6 2 29 2 0.0 6 2 30 2 0.0 6 2 31 1 0.0 6 1 32 2 0.0 6 1 1 33 1 0.0 6 1 34 4 0.0 6 4 35 2 0.0 6 2 36 1 0.0 6 0 1 38 4 0.0 6 4 39 1 0.0 6 1 41 2 0.0 6 2 42 3 0.0 6 1 1 1 43 3 0.0 6 3 44 7 0.0 6 7 45 2 0.0 6 2 46 3 0.0 6 3 47 4 0.0 6 3 0 1 48 2 0.0 6 1 0 0 1 49 1 0.0 6 1 50 2 0.0 6 2 51 1 0.0 6 1 52 1 0.0 6 1 53 4 0.0 6 4 54 4 0.0 6 4 55 2 0.0 6 2 56 3 0.0 6 2 1 57 1 0.0 6 1 61 3 0.0 6 3 62 2 0.0 6 2 63 2 0.0 6 2 64 2 0.0 6 2 65 1 0.0 6 1 66 2 0.0 6 2 67 4 0.0 6 4 68 4 0.0 6 4 69 2 0.0 6 1 1 71 1 0.0 6 1 72 1 0.0 6 1 73 4 0.0 6 4 75 3 0.0 6 3 76 5 0.0 6 5 77 7 0.0 6 7 78 2 0.0 6 2 79 2 0.0 6 2 81 1 0.0 6 1 82 2 0.0 6 2 83 1 0.0 6 1 84 4 0.0 6 4 85 2 0.0 6 1 1 86 7 0.0 6 7 87 6 0.0 6 6 88 1 0.0 6 1 89 7 0.0 6 7 90 2 0.0 6 2 91 1 0.0 6 1 92 5 0.0 6 5 93 2 0.0 6 2 94 1 0.0 6 1 96 2 0.0 6 2 97 3 0.0 6 2 1 98 10 0.0 6 10 100 4 0.0 6 4 101 7 0.0 6 6 1 102 2 0.0 6 2 103 1 0.0 6 1 104 6 0.0 6 5 1 105 8 0.0 6 8 106 5 0.0 6 5 107 4 0.0 6 3 0 0 1 108 11 0.0 6 9 2 109 4 0.0 6 4 110 5 0.0 6 4 0 1 111 5 0.0 6 5 112 12 0.0 6 11 1 113 3 0.0 6 3 114 3 0.0 6 3 115 12 0.0 6 12 116 7 0.0 6 7 117 6 0.0 6 6 118 2 0.0 6 2 119 10 0.0 6 9 1 120 2 0.0 6 2 121 8 0.0 6 8 122 12 0.0 6 12 123 15 0.0 6 15 124 4 0.0 6 4 125 10 0.0 6 9 1 126 17 0.0 6 17 127 5 0.0 6 5 128 20 0.0 6 20 129 13 0.0 6 13 130 6 0.0 6 6 131 11 0.0 6 11 132 5 0.0 6 5 133 6 0.0 6 6 134 11 0.0 6 10 1 135 14 0.0 6 14 136 10 0.0 6 10 137 10 0.0 6 10 138 12 0.0 6 12 139 9 0.0 6 9 140 15 0.0 6 15 141 17 0.0 6 15 2 142 21 0.0 6 21 143 64 0.0 6 62 2 144 97 0.0 6 97 145 96 0.0 6 96 146 57 0.0 6 57 147 78 0.0 6 77 1 148 114 0.0 6 112 2 149 101 0.0 6 100 1 150 89 0.0 6 89 151 97 0.0 6 95 2 152 82 0.0 6 80 1 1 153 138 0.0 6 138 154 96 0.0 6 96 155 80 0.0 6 79 0 1 156 53 0.0 6 53 157 66 0.0 6 65 1 158 65 0.0 6 65 159 60 0.0 6 60 160 80 0.0 6 80 161 77 0.0 6 74 2 0 1 162 82 0.0 6 82 163 92 0.0 6 92 164 70 0.0 6 69 1 165 98 0.0 6 97 1 166 78 0.0 6 78 167 51 0.0 6 49 2 168 56 0.0 6 55 1 169 94 0.0 6 93 1 170 54 0.0 6 54 171 63 0.0 6 63 172 54 0.0 6 54 173 70 0.0 6 70 174 45 0.0 6 45 175 85 0.0 6 82 3 176 76 0.0 6 75 1 177 74 0.0 6 74 178 61 0.0 6 60 1 179 88 0.0 6 87 1 180 72 0.0 6 71 1 181 206 0.0 6 203 3 182 113 0.0 6 112 1 183 88 0.0 6 88 184 74 0.0 6 74 185 88 0.0 6 87 1 186 98 0.0 6 95 3 187 109 0.0 6 105 3 1 188 112 0.0 6 110 2 189 90 0.0 6 90 190 102 0.0 6 99 3 191 72 0.0 6 71 1 192 121 0.0 6 119 2 193 100 0.0 6 99 1 194 176 0.0 6 173 3 195 173 0.0 6 170 3 196 203 0.0 6 200 2 1 197 239 0.0 6 235 4 198 241 0.0 6 236 5 199 232 0.0 6 230 2 200 166 0.0 6 161 5 201 203 0.0 6 200 3 202 161 0.0 6 156 5 203 187 0.0 6 185 1 1 204 205 0.0 6 204 1 205 271 0.0 6 268 3 206 248 0.0 6 247 1 207 309 0.0 6 305 4 208 243 0.0 6 238 5 209 235 0.0 6 233 2 210 223 0.0 6 216 5 2 211 227 0.0 6 222 5 212 295 0.0 6 292 3 213 273 0.0 6 265 8 214 245 0.0 6 244 1 215 241 0.0 6 239 2 216 233 0.0 6 231 2 217 204 0.0 6 203 1 218 202 0.0 6 198 4 219 197 0.0 6 193 4 220 139 0.0 6 137 2 221 167 0.0 6 167 222 200 0.0 6 196 3 1 223 190 0.0 6 188 2 224 190 0.0 6 185 5 225 344 0.0 6 337 7 226 288 0.0 6 283 5 227 259 0.0 6 257 2 228 203 0.0 6 198 5 229 197 0.0 6 195 2 230 237 0.0 6 232 5 231 214 0.0 6 209 5 232 376 0.0 6 374 2 233 285 0.0 6 281 4 234 193 0.0 6 185 8 235 314 0.0 6 310 4 236 196 0.0 6 188 7 0 0 1 237 498 0.0 6 488 10 238 315 0.0 6 311 4 239 313 0.0 6 305 8 240 401 0.0 6 395 6 241 342 0.0 6 337 5 242 518 0.0 6 504 13 1 243 533 0.0 6 523 10 244 692 0.0 6 679 12 0 1 245 440 0.0 6 432 8 246 565 0.0 6 553 12 247 382 0.0 6 370 12 248 615 0.0 6 602 13 249 376 0.0 6 370 6 250 250 0.0 6 244 6 251 474 0.0 6 463 11 252 436 0.0 6 432 4 253 246 0.0 6 235 11 254 525 0.0 6 513 11 1 255 300 0.0 6 289 11 256 344 0.0 6 339 5 257 309 0.0 6 303 6 258 361 0.0 6 358 2 1 259 364 0.0 6 357 7 260 572 0.0 6 564 8 261 355 0.0 6 347 8 262 353 0.0 6 345 8 263 630 0.0 6 615 15 264 432 0.0 6 425 7 265 509 0.0 6 503 6 266 511 0.0 6 492 18 1 267 458 0.0 6 453 5 268 306 0.0 6 300 6 269 565 0.0 6 559 5 1 270 347 0.0 6 339 8 271 408 0.0 6 397 9 1 0 1 272 556 0.0 6 542 14 273 695 0.0 6 676 18 1 274 1152 0.0 6 1119 31 2 275 1869 0.0 6 1822 46 0 1 276 1667 0.0 6 1645 21 0 0 0 0 1 277 1414 0.0 6 1366 45 2 1 278 6698 0.0 6 6562 134 2 279 1540 0.0 6 1472 65 3 280 28 0.0 6 25 2 1 281 3 0.0 6 2 1 282 2 0.0 6 1 0 0 0 1 283 1 0.0 6 1 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 209 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 13 0.0 6 13 72 3 0.0 6 3 73 3 0.0 6 3 74 4 0.0 6 4 75 1 0.0 6 1 76 2 0.0 6 2 77 2 0.0 6 2 78 1 0.0 6 1 80 7 0.0 6 7 81 1 0.0 6 1 82 3 0.0 6 3 85 2 0.0 6 2 86 1 0.0 6 1 88 1 0.0 6 1 89 1 0.0 6 1 90 2 0.0 6 2 91 2 0.0 6 2 92 5 0.0 6 5 93 2 0.0 6 2 94 2 0.0 6 2 95 1 0.0 6 1 96 1 0.0 6 1 97 3 0.0 6 3 98 1 0.0 6 1 100 2 0.0 6 2 101 1 0.0 6 1 102 1 0.0 6 1 104 1 0.0 6 1 105 2 0.0 6 2 107 1 0.0 6 1 108 1 0.0 6 1 109 1 0.0 6 1 110 2 0.0 6 2 111 1 0.0 6 1 112 1 0.0 6 1 113 1 0.0 6 1 115 1 0.0 6 1 116 2 0.0 6 2 117 1 0.0 6 1 118 1 0.0 6 1 121 3 0.0 6 3 122 1 0.0 6 1 123 1 0.0 6 1 124 1 0.0 6 1 125 1 0.0 6 1 126 2 0.0 6 2 128 3 0.0 6 3 131 2 0.0 6 2 133 4 0.0 6 4 134 1 0.0 6 1 136 1 0.0 6 1 137 1 0.0 6 1 139 1 0.0 6 1 140 1 0.0 6 1 141 2 0.0 6 2 142 1 0.0 6 1 144 2 0.0 6 2 145 2 0.0 6 2 146 1 0.0 6 1 148 6 0.0 6 6 149 1 0.0 6 1 150 1 0.0 6 1 151 2 0.0 6 2 153 3 0.0 6 3 157 1 0.0 6 1 158 2 0.0 6 2 160 1 0.0 6 1 161 1 0.0 6 1 162 2 0.0 6 2 163 2 0.0 6 2 164 3 0.0 6 3 165 2 0.0 6 2 166 1 0.0 6 1 167 3 0.0 6 3 168 2 0.0 6 2 169 2 0.0 6 2 170 3 0.0 6 3 171 2 0.0 6 2 172 3 0.0 6 3 173 4 0.0 6 4 174 1 0.0 6 1 175 1 0.0 6 1 176 1 0.0 6 1 177 1 0.0 6 1 178 2 0.0 6 2 182 1 0.0 6 1 184 1 0.0 6 1 186 2 0.0 6 2 188 1 0.0 6 1 190 1 0.0 6 1 192 2 0.0 6 2 193 1 0.0 6 1 194 1 0.0 6 1 198 1 0.0 6 1 200 1 0.0 6 1 201 3 0.0 6 3 204 1 0.0 6 1 205 1 0.0 6 1 206 1 0.0 6 1 208 1 0.0 6 1 212 1 0.0 6 1 213 2 0.0 6 2 214 1 0.0 6 1 216 1 0.0 6 1 218 1 0.0 6 1 219 1 0.0 6 1 224 1 0.0 6 1 225 5 0.0 6 5 226 1 0.0 6 1 229 1 0.0 6 1 231 1 0.0 6 1 243 1 0.0 6 1 248 1 0.0 6 1 251 1 0.0 6 1 259 1 0.0 6 1 264 1 0.0 6 1 288 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 45449 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 9 1 0.2 2 1 10 1 0.0 3 0 1 11 3 0.0 3 2 1 13 6 0.0 3 5 1 14 3 0.0 4 3 15 1 0.0 4 0 1 16 7 0.0 4 6 1 17 6 0.0 5 6 18 8 0.0 5 8 19 4 0.0 5 4 20 40 0.0 6 38 2 21 34 0.0 6 33 0 1 22 12 0.0 6 12 23 15 0.0 6 14 1 24 19 0.0 6 19 25 12 0.0 6 11 0 0 1 26 11 0.0 6 8 3 27 5 0.0 6 4 1 28 6 0.0 6 6 29 12 0.0 6 11 1 30 4 0.0 6 4 31 10 0.0 6 9 1 32 13 0.0 6 13 33 2 0.0 6 1 1 34 4 0.0 6 3 0 1 35 3 0.0 6 3 36 4 0.0 6 3 1 37 7 0.0 6 7 38 2 0.0 6 2 39 4 0.0 6 4 40 3 0.0 6 3 41 6 0.0 6 4 2 42 4 0.0 6 2 1 1 43 7 0.0 6 5 1 0 0 0 0 1 44 10 0.0 6 10 45 1 0.0 6 1 46 14 0.0 6 14 47 12 0.0 6 8 4 48 3 0.0 6 3 49 14 0.0 6 12 1 1 50 12 0.0 6 12 51 6 0.0 6 6 52 19 0.0 6 17 2 53 19 0.0 6 19 54 12 0.0 6 11 1 55 10 0.0 6 8 2 56 13 0.0 6 12 1 57 9 0.0 6 8 1 58 15 0.0 6 14 1 59 5 0.0 6 5 60 12 0.0 6 12 61 14 0.0 6 13 1 62 5 0.0 6 2 3 63 6 0.0 6 6 64 12 0.0 6 12 65 9 0.0 6 6 3 66 17 0.0 6 15 2 67 72 0.0 6 71 0 0 1 68 23 0.0 6 22 0 1 69 44 0.0 6 42 2 70 6 0.0 6 5 1 71 20 0.0 6 20 72 13 0.0 6 13 73 12 0.0 6 10 1 0 0 1 74 12 0.0 6 11 1 75 8 0.0 6 8 76 13 0.0 6 12 1 77 12 0.0 6 11 0 0 0 1 78 10 0.0 6 9 1 79 15 0.0 6 14 0 1 80 17 0.0 6 15 2 81 16 0.0 6 14 2 82 22 0.0 6 22 83 49 0.0 6 45 4 84 24 0.0 6 23 1 85 12 0.0 6 12 86 22 0.0 6 19 2 1 87 24 0.0 6 21 2 0 1 88 18 0.0 6 16 1 0 0 1 89 24 0.0 6 22 2 90 25 0.0 6 21 4 91 33 0.0 6 31 2 92 40 0.0 6 37 2 1 93 37 0.0 6 28 5 4 94 52 0.0 6 48 3 1 95 32 0.0 6 29 3 96 35 0.0 6 32 3 97 40 0.0 6 36 4 98 27 0.0 6 25 0 1 0 1 99 30 0.0 6 28 2 100 36 0.0 6 33 3 101 32 0.0 6 30 2 102 42 0.0 6 42 103 52 0.0 6 49 3 104 51 0.0 6 47 3 0 1 105 46 0.0 6 44 2 106 22 0.0 6 22 107 44 0.0 6 41 3 108 28 0.0 6 27 0 1 109 37 0.0 6 36 1 110 35 0.0 6 35 111 42 0.0 6 41 1 112 37 0.0 6 35 1 0 0 0 1 113 37 0.0 6 37 114 56 0.0 6 56 115 41 0.0 6 39 2 116 59 0.0 6 57 1 1 117 34 0.0 6 31 3 118 66 0.0 6 64 2 119 38 0.0 6 37 1 120 66 0.0 6 64 1 1 121 67 0.0 6 66 1 122 61 0.0 6 57 4 123 72 0.0 6 69 2 0 0 0 1 124 69 0.0 6 67 2 125 78 0.0 6 74 4 126 78 0.0 6 74 3 0 0 1 127 77 0.0 6 74 3 128 95 0.0 6 93 2 129 56 0.0 6 51 4 0 0 0 1 130 74 0.0 6 72 1 0 0 1 131 97 0.0 6 94 3 132 115 0.0 6 112 3 133 131 0.0 6 128 2 0 0 1 134 156 0.0 6 154 2 135 193 0.0 6 186 6 0 1 136 168 0.0 6 160 6 0 0 0 1 1 137 199 0.0 6 194 5 138 119 0.0 6 113 6 139 186 0.0 6 179 7 140 222 0.0 6 216 4 2 141 262 0.0 6 254 8 142 314 0.0 6 302 12 143 268 0.0 6 260 8 144 301 0.0 6 292 8 1 145 247 0.0 6 242 4 0 0 0 1 146 244 0.0 6 240 2 0 0 1 1 147 215 0.0 6 211 4 148 289 0.0 6 283 4 0 0 1 0 1 149 410 0.0 6 399 11 150 591 0.0 6 571 19 0 0 1 151 259 0.0 6 249 9 0 1 152 458 0.0 6 442 14 0 0 1 0 1 153 374 0.0 6 371 2 0 0 0 1 154 411 0.0 6 405 5 0 1 155 386 0.0 6 374 10 2 156 330 0.0 6 322 8 157 489 0.0 6 477 10 1 0 0 0 1 158 434 0.0 6 425 8 0 0 0 0 1 159 272 0.0 6 268 4 160 363 0.0 6 362 1 161 259 0.0 6 256 2 1 162 219 0.0 6 209 10 163 320 0.0 6 311 8 0 0 1 164 396 0.0 6 393 3 165 354 0.0 6 346 6 2 166 440 0.0 6 432 6 1 0 1 167 239 0.0 6 232 6 1 168 593 0.0 6 579 14 169 271 0.0 6 265 5 1 170 294 0.0 6 287 7 171 651 0.0 6 646 5 172 285 0.0 6 278 4 1 2 173 398 0.0 6 395 2 1 174 627 0.0 6 613 14 175 713 0.0 6 695 16 2 176 476 0.0 6 468 8 177 456 0.0 6 446 9 0 0 1 178 380 0.0 6 368 11 1 179 677 0.0 6 656 17 4 180 334 0.0 6 328 6 181 462 0.0 6 453 8 1 182 124 0.0 6 121 3 183 353 0.0 6 345 8 184 388 0.0 6 378 10 185 575 0.0 6 562 11 2 186 867 0.0 6 846 20 0 0 1 187 762 0.0 6 736 23 1 1 1 188 590 0.0 6 573 16 0 0 1 189 623 0.0 6 610 13 190 765 0.0 6 744 19 1 0 1 191 844 0.0 6 822 21 0 0 1 192 557 0.0 6 540 14 3 193 535 0.0 6 518 16 1 194 372 0.0 6 364 8 195 551 0.0 6 531 19 0 0 1 196 1237 0.0 6 1205 32 197 575 0.0 6 561 12 1 0 0 0 1 198 644 0.0 6 616 26 1 0 1 199 1035 0.0 6 1009 25 0 0 1 200 466 0.0 6 450 15 1 201 761 0.0 6 740 19 1 0 0 1 202 345 0.0 6 333 11 1 203 389 0.0 6 375 13 1 204 343 0.0 6 335 8 205 232 0.0 6 226 6 206 282 0.0 6 278 4 207 235 0.0 6 230 5 208 270 0.0 6 265 5 209 198 0.0 6 191 6 1 210 286 0.0 6 274 11 1 211 574 0.0 6 564 10 212 534 0.0 6 524 9 0 1 213 135 0.0 6 131 4 214 629 0.0 6 613 15 0 0 1 215 1228 0.0 6 1205 21 0 0 2 216 389 0.0 6 375 14 217 600 0.0 6 584 14 1 1 218 255 0.0 6 249 3 3 219 229 0.0 6 223 6 220 64 0.0 6 62 2 221 285 0.0 6 277 8 222 574 0.0 6 554 20 223 596 0.0 6 579 17 224 352 0.0 6 347 5 225 298 0.0 6 289 9 226 166 0.0 6 163 3 227 176 0.0 6 172 3 0 0 1 228 73 0.0 6 70 3 229 199 0.0 6 193 4 1 1 230 257 0.0 6 248 7 0 0 2 231 216 0.0 6 210 6 232 428 0.0 6 413 14 0 0 1 233 114 0.0 6 110 3 0 0 1 234 108 0.0 6 104 4 235 114 0.0 6 111 2 1 236 65 0.0 6 64 1 237 37 0.0 6 37 238 41 0.0 6 40 1 239 126 0.0 6 124 2 240 153 0.0 6 150 3 241 222 0.0 6 220 2 242 171 0.0 6 170 1 243 97 0.0 6 93 4 244 81 0.0 6 81 245 48 0.0 6 46 2 246 34 0.0 6 33 1 247 22 0.0 6 20 2 248 63 0.0 6 60 3 249 27 0.0 6 27 250 27 0.0 6 26 1 251 25 0.0 6 25 252 12 0.0 6 11 1 253 16 0.0 6 15 1 254 4 0.0 6 4 257 1 0.0 6 1 258 1 0.0 6 1 261 1 0.0 6 1 277 1 0.0 6 1 278 12 0.0 6 10 2 279 23 0.0 6 23 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 168 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 73 2 0.0 6 2 74 2 0.0 6 2 76 3 0.0 6 3 77 4 0.0 6 4 78 1 0.0 6 1 79 4 0.0 6 3 1 80 2 0.0 6 2 82 1 0.0 6 1 83 2 0.0 6 2 84 2 0.0 6 2 85 2 0.0 6 2 86 4 0.0 6 4 87 2 0.0 6 2 88 3 0.0 6 3 89 1 0.0 6 1 90 2 0.0 6 2 91 4 0.0 6 4 92 3 0.0 6 3 93 1 0.0 6 1 94 3 0.0 6 3 95 1 0.0 6 1 97 1 0.0 6 1 102 1 0.0 6 1 104 2 0.0 6 2 105 1 0.0 6 1 106 3 0.0 6 3 107 1 0.0 6 1 108 1 0.0 6 1 109 1 0.0 6 1 110 8 0.0 6 8 111 1 0.0 6 1 113 1 0.0 6 1 115 1 0.0 6 1 116 2 0.0 6 2 117 1 0.0 6 1 119 1 0.0 6 1 120 1 0.0 6 1 121 2 0.0 6 2 122 2 0.0 6 2 123 1 0.0 6 1 125 2 0.0 6 2 126 3 0.0 6 3 127 5 0.0 6 5 128 2 0.0 6 2 130 1 0.0 6 1 132 1 0.0 6 1 133 1 0.0 6 1 134 1 0.0 6 1 136 2 0.0 6 2 137 2 0.0 6 2 139 1 0.0 6 1 141 3 0.0 6 3 143 1 0.0 6 1 144 1 0.0 6 1 145 2 0.0 6 2 146 1 0.0 6 1 147 3 0.0 6 3 150 3 0.0 6 3 152 2 0.0 6 2 156 1 0.0 6 1 157 1 0.0 6 1 158 3 0.0 6 3 159 1 0.0 6 1 160 5 0.0 6 5 161 2 0.0 6 2 162 2 0.0 6 2 163 3 0.0 6 3 165 2 0.0 6 2 167 1 0.0 6 1 168 1 0.0 6 1 170 1 0.0 6 1 171 1 0.0 6 1 172 3 0.0 6 3 173 2 0.0 6 2 174 2 0.0 6 2 180 2 0.0 6 2 183 1 0.0 6 1 186 3 0.0 6 3 188 1 0.0 6 1 190 1 0.0 6 1 192 1 0.0 6 1 194 1 0.0 6 1 202 1 0.0 6 1 204 1 0.0 6 1 205 1 0.0 6 1 208 1 0.0 6 1 225 1 0.0 6 1 226 3 0.0 6 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_01_12_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_01_239_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_01_12_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_01_239_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_02_13_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_02_240_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_02_13_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_02_240_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_03_14_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_03_241_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_03_14_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_03_241_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_04_15_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_04_242_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_04_15_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_04_242_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_05_16_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_05_243_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_05_16_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_05_243_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_06_17_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_06_244_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_06_17_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_06_244_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_15_18_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_15_245_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_15_18_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_15_245_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_16_19_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_16_246_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_16_19_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_16_246_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_17_20_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_17_247_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_17_20_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_17_247_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_18_21_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_18_248_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_18_21_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_18_248_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_19_22_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_19_249_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_19_22_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_19_249_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_20_23_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_02-Rev_20_250_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_20_23_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_02-Rev_20_250_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_01_24_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_01_251_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_01_24_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_01_251_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.77 s (24 µs/read; 2.55 M reads/minute). === Summary === Total read pairs processed: 75,211 Read 1 with adapter: 75,200 (100.0%) Read 2 with adapter: 75,143 (99.9%) == Read fate breakdown == Pairs that were too short: 46 (0.1%) Pairs discarded as untrimmed: 78 (0.1%) Pairs written (passing filters): 75,087 (99.8%) Total basepairs processed: 32,506,409 bp Read 1: 18,573,265 bp Read 2: 13,933,144 bp Total written (filtered): 4,822,178 bp (14.8%) Read 1: 2,538,655 bp Read 2: 2,283,523 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 73653 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 1175.2 0 1 3 4 7 293.8 1 1 6 7 4 4.6 2 2 0 2 15 2 0.0 4 2 16 1 0.0 4 0 1 17 2 0.0 5 2 18 1 0.0 5 1 19 3 0.0 5 1 2 20 107 0.0 6 106 1 21 59 0.0 6 58 1 22 46 0.0 6 46 23 36 0.0 6 34 2 24 21 0.0 6 20 1 25 27 0.0 6 26 1 26 14 0.0 6 12 1 0 0 0 1 27 8 0.0 6 8 28 8 0.0 6 8 29 15 0.0 6 14 1 30 7 0.0 6 7 31 3 0.0 6 2 1 32 7 0.0 6 7 33 6 0.0 6 6 34 7 0.0 6 7 35 2 0.0 6 2 36 6 0.0 6 6 37 6 0.0 6 5 1 38 6 0.0 6 4 2 39 11 0.0 6 11 40 8 0.0 6 7 1 41 15 0.0 6 15 42 6 0.0 6 5 1 43 5 0.0 6 4 1 44 10 0.0 6 10 45 3 0.0 6 3 46 5 0.0 6 5 47 4 0.0 6 4 48 8 0.0 6 7 1 49 10 0.0 6 10 50 6 0.0 6 5 1 51 8 0.0 6 8 52 4 0.0 6 4 53 6 0.0 6 5 1 54 4 0.0 6 3 0 1 55 4 0.0 6 3 0 0 1 56 4 0.0 6 3 1 57 3 0.0 6 3 58 5 0.0 6 5 59 1 0.0 6 1 60 7 0.0 6 7 61 9 0.0 6 9 62 4 0.0 6 4 63 5 0.0 6 5 64 6 0.0 6 6 65 11 0.0 6 11 66 4 0.0 6 4 67 2 0.0 6 2 68 11 0.0 6 11 69 14 0.0 6 14 70 9 0.0 6 9 71 6 0.0 6 5 1 72 13 0.0 6 13 73 9 0.0 6 9 74 13 0.0 6 13 75 8 0.0 6 8 76 4 0.0 6 4 77 4 0.0 6 3 1 78 8 0.0 6 8 79 3 0.0 6 3 80 11 0.0 6 10 1 81 6 0.0 6 6 82 14 0.0 6 14 83 14 0.0 6 13 1 84 15 0.0 6 15 85 4 0.0 6 4 86 13 0.0 6 12 0 0 0 0 1 87 8 0.0 6 8 88 12 0.0 6 12 89 10 0.0 6 10 90 4 0.0 6 4 91 10 0.0 6 9 1 92 6 0.0 6 6 93 17 0.0 6 15 1 1 94 10 0.0 6 10 95 6 0.0 6 6 96 7 0.0 6 7 97 19 0.0 6 18 1 98 13 0.0 6 12 0 0 1 99 14 0.0 6 14 100 19 0.0 6 18 1 101 11 0.0 6 10 0 0 0 0 0 1 102 15 0.0 6 14 0 0 0 1 103 24 0.0 6 23 1 104 18 0.0 6 18 105 20 0.0 6 19 1 106 14 0.0 6 13 1 107 20 0.0 6 20 108 18 0.0 6 18 109 25 0.0 6 25 110 22 0.0 6 22 111 32 0.0 6 31 1 112 20 0.0 6 19 1 113 20 0.0 6 20 114 22 0.0 6 21 1 115 27 0.0 6 25 2 116 32 0.0 6 32 117 23 0.0 6 23 118 35 0.0 6 34 0 0 0 1 119 24 0.0 6 23 1 120 28 0.0 6 27 1 121 15 0.0 6 13 2 122 30 0.0 6 30 123 33 0.0 6 32 0 1 124 27 0.0 6 27 125 22 0.0 6 21 1 126 36 0.0 6 33 2 0 0 0 0 1 127 38 0.0 6 38 128 34 0.0 6 34 129 35 0.0 6 33 2 130 39 0.0 6 38 1 131 26 0.0 6 26 132 44 0.0 6 42 2 133 46 0.0 6 44 1 1 134 42 0.0 6 41 0 0 0 0 1 135 38 0.0 6 37 1 136 32 0.0 6 31 1 137 43 0.0 6 43 138 42 0.0 6 41 0 1 139 68 0.0 6 67 1 140 88 0.0 6 88 141 282 0.0 6 280 1 0 0 1 142 421 0.0 6 420 1 143 572 0.0 6 571 1 144 275 0.0 6 272 2 0 0 0 1 145 329 0.0 6 328 1 146 464 0.0 6 464 147 390 0.0 6 388 2 148 376 0.0 6 375 1 149 361 0.0 6 358 2 0 1 150 393 0.0 6 392 1 151 305 0.0 6 305 152 291 0.0 6 290 1 153 206 0.0 6 202 4 154 270 0.0 6 268 2 155 304 0.0 6 301 2 0 1 156 232 0.0 6 232 157 225 0.0 6 224 1 158 287 0.0 6 284 2 1 159 287 0.0 6 285 2 160 233 0.0 6 233 161 310 0.0 6 303 6 0 0 0 0 1 162 297 0.0 6 294 3 163 323 0.0 6 321 1 1 164 353 0.0 6 352 1 165 211 0.0 6 206 4 1 166 217 0.0 6 216 1 167 236 0.0 6 232 3 0 0 0 1 168 233 0.0 6 232 1 169 251 0.0 6 251 170 308 0.0 6 306 2 171 277 0.0 6 275 1 0 0 0 1 172 223 0.0 6 222 1 173 275 0.0 6 272 3 174 280 0.0 6 278 1 0 0 0 1 175 301 0.0 6 295 5 1 176 311 0.0 6 305 4 0 2 177 264 0.0 6 262 2 178 329 0.0 6 326 3 179 873 0.0 6 870 3 180 370 0.0 6 367 3 181 310 0.0 6 306 3 1 182 304 0.0 6 298 6 183 325 0.0 6 321 3 0 0 0 1 184 332 0.0 6 326 4 0 0 2 185 382 0.0 6 378 2 0 0 2 186 362 0.0 6 359 3 187 322 0.0 6 317 5 188 380 0.0 6 376 4 189 315 0.0 6 313 2 190 358 0.0 6 356 2 191 445 0.0 6 436 8 0 0 0 1 192 441 0.0 6 434 6 1 193 539 0.0 6 534 2 0 0 1 2 194 661 0.0 6 656 5 195 653 0.0 6 645 8 196 726 0.0 6 720 5 0 0 0 1 197 910 0.0 6 898 10 2 198 605 0.0 6 596 8 0 1 199 541 0.0 6 534 6 0 1 200 627 0.0 6 619 6 1 1 201 525 0.0 6 515 6 2 1 0 1 202 675 0.0 6 664 10 1 203 777 0.0 6 769 7 0 0 0 1 204 706 0.0 6 700 4 1 0 0 0 1 205 936 0.0 6 921 11 2 1 0 1 206 738 0.0 6 731 7 207 781 0.0 6 770 10 0 0 0 0 1 208 687 0.0 6 678 8 1 209 667 0.0 6 661 5 0 0 0 1 210 811 0.0 6 801 8 2 211 804 0.0 6 795 9 212 787 0.0 6 778 9 213 876 0.0 6 863 13 214 1045 0.0 6 1039 6 215 839 0.0 6 830 9 216 808 0.0 6 796 10 1 0 0 0 1 217 902 0.0 6 895 6 1 218 818 0.0 6 811 5 2 219 666 0.0 6 658 7 0 0 0 1 220 675 0.0 6 668 6 0 1 221 640 0.0 6 630 9 1 222 699 0.0 6 690 8 1 223 634 0.0 6 628 5 0 1 224 805 0.0 6 800 5 225 864 0.0 6 850 13 1 226 742 0.0 6 736 5 0 1 227 714 0.0 6 706 7 1 228 730 0.0 6 722 7 0 0 1 229 651 0.0 6 647 4 230 680 0.0 6 675 5 231 684 0.0 6 673 9 1 0 1 232 690 0.0 6 682 7 1 233 664 0.0 6 654 9 1 234 566 0.0 6 564 2 235 589 0.0 6 586 2 0 0 0 0 1 236 671 0.0 6 663 7 1 237 641 0.0 6 636 5 238 584 0.0 6 580 3 0 0 0 0 1 239 660 0.0 6 656 4 240 649 0.0 6 641 6 1 0 0 1 241 588 0.0 6 579 8 1 242 570 0.0 6 564 6 243 567 0.0 6 563 4 244 493 0.0 6 490 2 1 245 413 0.0 6 410 3 246 393 0.0 6 389 4 247 435 0.0 6 426 8 0 0 1 248 426 0.0 6 419 7 249 490 0.0 6 486 4 250 477 0.0 6 471 5 1 251 377 0.0 6 375 2 252 400 0.0 6 389 10 0 0 1 253 382 0.0 6 379 3 254 407 0.0 6 402 4 1 255 381 0.0 6 379 2 256 377 0.0 6 375 2 257 391 0.0 6 388 3 258 457 0.0 6 453 3 1 259 421 0.0 6 417 3 0 0 0 1 260 266 0.0 6 265 1 261 269 0.0 6 267 0 1 1 262 305 0.0 6 302 3 263 317 0.0 6 315 2 264 298 0.0 6 295 3 265 275 0.0 6 271 3 0 1 266 289 0.0 6 288 1 267 369 0.0 6 366 3 268 350 0.0 6 346 4 269 304 0.0 6 302 2 270 320 0.0 6 317 3 271 297 0.0 6 294 3 272 358 0.0 6 350 7 0 0 0 0 1 273 369 0.0 6 367 2 274 423 0.0 6 417 5 1 275 505 0.0 6 499 6 276 677 0.0 6 669 6 2 277 2537 0.0 6 2514 17 4 0 0 1 1 278 3083 0.0 6 3026 53 1 3 279 72 0.0 6 63 9 280 7 0.0 6 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1547 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 59 1 0.0 6 1 64 9 0.0 6 9 65 1 0.0 6 1 69 1 0.0 6 1 70 1 0.0 6 0 0 0 0 0 0 1 72 5 0.0 6 5 73 9 0.0 6 8 1 74 21 0.0 6 21 75 23 0.0 6 23 76 11 0.0 6 11 77 17 0.0 6 17 78 12 0.0 6 12 79 13 0.0 6 13 80 9 0.0 6 9 81 8 0.0 6 8 82 8 0.0 6 8 83 9 0.0 6 9 84 7 0.0 6 7 85 9 0.0 6 8 0 0 0 0 0 1 86 7 0.0 6 7 87 4 0.0 6 4 88 5 0.0 6 5 89 7 0.0 6 7 90 8 0.0 6 8 91 4 0.0 6 4 92 7 0.0 6 7 93 8 0.0 6 8 94 8 0.0 6 8 95 7 0.0 6 7 96 9 0.0 6 9 97 5 0.0 6 5 98 3 0.0 6 3 99 6 0.0 6 6 100 3 0.0 6 3 101 13 0.0 6 13 102 6 0.0 6 6 103 3 0.0 6 3 104 5 0.0 6 5 105 8 0.0 6 8 106 2 0.0 6 2 107 6 0.0 6 6 108 1 0.0 6 1 109 3 0.0 6 3 110 17 0.0 6 17 111 20 0.0 6 20 112 8 0.0 6 8 113 6 0.0 6 6 114 6 0.0 6 6 115 1 0.0 6 1 116 7 0.0 6 7 117 5 0.0 6 5 118 7 0.0 6 7 119 9 0.0 6 9 120 7 0.0 6 7 121 7 0.0 6 7 122 10 0.0 6 10 123 9 0.0 6 9 124 5 0.0 6 5 125 9 0.0 6 9 126 4 0.0 6 4 127 5 0.0 6 5 128 11 0.0 6 11 129 6 0.0 6 6 130 3 0.0 6 3 131 5 0.0 6 5 132 6 0.0 6 6 133 6 0.0 6 6 134 9 0.0 6 9 135 11 0.0 6 11 136 6 0.0 6 6 137 13 0.0 6 13 138 8 0.0 6 8 139 7 0.0 6 7 140 8 0.0 6 8 141 5 0.0 6 5 142 6 0.0 6 5 1 143 3 0.0 6 3 144 8 0.0 6 8 145 3 0.0 6 3 146 6 0.0 6 6 147 7 0.0 6 7 148 3 0.0 6 3 149 8 0.0 6 8 150 7 0.0 6 7 151 6 0.0 6 6 152 9 0.0 6 9 153 3 0.0 6 3 154 12 0.0 6 11 1 155 11 0.0 6 11 156 11 0.0 6 11 157 4 0.0 6 4 158 10 0.0 6 10 159 5 0.0 6 5 160 5 0.0 6 5 161 6 0.0 6 6 162 6 0.0 6 6 163 8 0.0 6 8 164 8 0.0 6 8 165 6 0.0 6 5 0 0 0 0 0 1 166 12 0.0 6 12 167 5 0.0 6 5 168 15 0.0 6 15 169 7 0.0 6 7 170 9 0.0 6 9 171 6 0.0 6 6 172 10 0.0 6 10 173 7 0.0 6 7 174 6 0.0 6 6 175 4 0.0 6 4 176 11 0.0 6 11 177 6 0.0 6 6 178 8 0.0 6 8 179 4 0.0 6 4 180 4 0.0 6 4 181 12 0.0 6 12 182 5 0.0 6 5 183 6 0.0 6 6 184 4 0.0 6 4 185 6 0.0 6 6 186 6 0.0 6 6 187 12 0.0 6 12 188 8 0.0 6 8 189 9 0.0 6 9 190 7 0.0 6 7 191 13 0.0 6 13 192 13 0.0 6 13 193 7 0.0 6 7 194 11 0.0 6 11 195 11 0.0 6 11 196 6 0.0 6 6 197 2 0.0 6 2 198 8 0.0 6 8 199 12 0.0 6 12 200 6 0.0 6 6 201 12 0.0 6 12 202 6 0.0 6 6 203 6 0.0 6 6 204 13 0.0 6 13 205 10 0.0 6 10 206 8 0.0 6 8 207 8 0.0 6 8 208 7 0.0 6 7 209 13 0.0 6 13 210 6 0.0 6 6 211 8 0.0 6 8 212 10 0.0 6 10 213 15 0.0 6 15 214 9 0.0 6 9 215 5 0.0 6 5 216 6 0.0 6 6 217 7 0.0 6 7 218 17 0.0 6 17 219 7 0.0 6 7 220 12 0.0 6 12 221 14 0.0 6 14 222 11 0.0 6 11 223 4 0.0 6 4 224 12 0.0 6 12 225 6 0.0 6 6 226 8 0.0 6 8 227 2 0.0 6 2 228 8 0.0 6 8 229 13 0.0 6 13 230 10 0.0 6 10 231 5 0.0 6 5 232 2 0.0 6 2 233 7 0.0 6 7 234 11 0.0 6 11 235 8 0.0 6 8 236 6 0.0 6 6 237 16 0.0 6 16 238 5 0.0 6 5 239 5 0.0 6 5 240 13 0.0 6 13 241 8 0.0 6 8 242 12 0.0 6 12 243 9 0.0 6 9 244 12 0.0 6 10 2 245 4 0.0 6 4 246 4 0.0 6 4 247 6 0.0 6 6 248 6 0.0 6 6 249 2 0.0 6 2 250 4 0.0 6 4 251 6 0.0 6 6 252 3 0.0 6 3 253 8 0.0 6 8 254 9 0.0 6 9 255 2 0.0 6 2 256 3 0.0 6 3 257 6 0.0 6 5 1 258 4 0.0 6 4 259 4 0.0 6 4 260 9 0.0 6 9 261 2 0.0 6 2 262 4 0.0 6 4 263 1 0.0 6 1 264 5 0.0 6 5 266 5 0.0 6 5 267 3 0.0 6 3 268 3 0.0 6 3 269 3 0.0 6 3 270 3 0.0 6 3 271 3 0.0 6 3 272 2 0.0 6 2 273 2 0.0 6 2 274 1 0.0 6 1 275 4 0.0 6 4 276 2 0.0 6 2 277 2 0.0 6 2 278 2 0.0 6 2 279 2 0.0 6 2 280 2 0.0 6 2 283 2 0.0 6 1 0 0 0 0 0 1 284 3 0.0 6 3 285 3 0.0 6 3 287 2 0.0 6 2 288 4 0.0 6 4 289 1 0.0 6 1 290 1 0.0 6 1 291 1 0.0 6 1 293 2 0.0 6 2 294 1 0.0 6 1 295 1 0.0 6 1 297 2 0.0 6 2 298 1 0.0 6 1 303 2 0.0 6 2 307 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 74106 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 293.8 1 1 6 1 18.4 1 1 10 2 0.1 3 2 11 4 0.0 3 3 1 12 6 0.0 3 5 0 1 13 14 0.0 3 14 14 8 0.0 4 7 1 15 1 0.0 4 1 16 21 0.0 4 20 1 17 20 0.0 5 19 1 18 13 0.0 5 12 1 19 25 0.0 5 22 3 20 132 0.0 6 128 3 1 21 81 0.0 6 77 4 22 57 0.0 6 56 1 23 31 0.0 6 31 24 35 0.0 6 35 25 29 0.0 6 27 2 26 29 0.0 6 25 3 0 1 27 26 0.0 6 23 2 1 28 19 0.0 6 17 2 29 21 0.0 6 17 3 1 30 29 0.0 6 27 1 1 31 26 0.0 6 24 2 32 19 0.0 6 19 33 21 0.0 6 19 2 34 23 0.0 6 23 35 14 0.0 6 12 2 36 21 0.0 6 21 37 19 0.0 6 17 1 0 0 0 0 1 38 15 0.0 6 15 39 22 0.0 6 21 1 40 19 0.0 6 18 1 41 19 0.0 6 18 0 1 42 25 0.0 6 21 4 43 12 0.0 6 10 2 44 22 0.0 6 22 45 25 0.0 6 22 3 46 26 0.0 6 24 2 47 27 0.0 6 27 48 24 0.0 6 19 3 0 0 0 2 49 21 0.0 6 20 1 50 31 0.0 6 30 1 51 24 0.0 6 23 1 52 25 0.0 6 24 1 53 40 0.0 6 37 2 0 1 54 27 0.0 6 23 4 55 30 0.0 6 26 4 56 32 0.0 6 27 4 1 57 20 0.0 6 20 58 36 0.0 6 33 3 59 27 0.0 6 26 1 60 32 0.0 6 30 1 1 61 30 0.0 6 29 1 62 37 0.0 6 34 3 63 20 0.0 6 17 3 64 35 0.0 6 34 1 65 35 0.0 6 33 2 66 48 0.0 6 41 5 1 0 1 67 50 0.0 6 48 1 1 68 80 0.0 6 79 1 69 147 0.0 6 140 6 0 0 0 0 1 70 77 0.0 6 73 4 71 102 0.0 6 96 4 0 1 1 72 49 0.0 6 46 3 73 63 0.0 6 62 1 74 49 0.0 6 47 2 75 46 0.0 6 44 1 0 0 0 1 76 55 0.0 6 54 1 77 59 0.0 6 56 2 1 78 67 0.0 6 63 3 0 0 0 1 79 70 0.0 6 65 5 80 63 0.0 6 60 3 81 74 0.0 6 72 2 82 75 0.0 6 72 3 83 79 0.0 6 78 1 84 74 0.0 6 70 3 0 0 1 85 103 0.0 6 96 5 1 1 86 74 0.0 6 70 2 2 87 60 0.0 6 56 4 88 83 0.0 6 80 3 89 74 0.0 6 71 3 90 71 0.0 6 69 1 0 1 91 93 0.0 6 89 1 2 0 1 92 84 0.0 6 80 1 1 0 2 93 106 0.0 6 101 3 1 0 0 1 94 107 0.0 6 101 5 1 95 102 0.0 6 98 2 0 2 96 84 0.0 6 81 2 1 97 123 0.0 6 116 7 98 161 0.0 6 152 7 1 0 1 99 108 0.0 6 104 3 0 0 0 0 1 100 116 0.0 6 112 3 0 0 0 1 101 107 0.0 6 101 5 0 0 0 1 102 118 0.0 6 113 5 103 125 0.0 6 121 3 0 0 0 0 1 104 123 0.0 6 121 1 0 0 0 1 105 151 0.0 6 147 3 0 0 0 1 106 272 0.0 6 264 7 1 107 126 0.0 6 121 4 0 0 0 1 108 151 0.0 6 146 4 1 109 183 0.0 6 175 8 110 161 0.0 6 158 3 111 193 0.0 6 185 7 0 1 112 174 0.0 6 172 1 0 0 0 0 1 113 202 0.0 6 200 2 114 234 0.0 6 226 7 0 0 1 115 189 0.0 6 186 2 0 0 1 116 224 0.0 6 211 11 1 0 0 1 117 227 0.0 6 219 7 0 0 0 0 1 118 204 0.0 6 198 6 119 256 0.0 6 249 5 0 1 0 1 120 263 0.0 6 253 10 121 264 0.0 6 256 7 0 0 1 122 254 0.0 6 253 1 123 233 0.0 6 226 7 124 260 0.0 6 255 4 0 1 125 307 0.0 6 300 7 126 298 0.0 6 293 5 127 395 0.0 6 385 7 0 1 1 1 128 370 0.0 6 356 14 129 373 0.0 6 366 5 0 1 1 130 431 0.0 6 417 13 0 0 1 131 441 0.0 6 423 18 132 450 0.0 6 434 12 1 1 1 1 133 548 0.0 6 537 8 1 0 2 134 559 0.0 6 542 15 0 2 135 551 0.0 6 541 9 1 136 640 0.0 6 617 20 1 2 137 639 0.0 6 624 12 1 0 1 0 1 138 543 0.0 6 530 11 0 0 0 1 1 139 579 0.0 6 559 17 0 1 1 1 140 609 0.0 6 598 7 2 2 141 828 0.0 6 814 12 0 1 1 142 976 0.0 6 953 20 0 0 0 2 1 143 1109 0.0 6 1093 10 2 2 2 144 995 0.0 6 970 22 1 2 145 977 0.0 6 948 25 1 0 0 2 1 146 1144 0.0 6 1117 23 1 0 0 1 2 147 1093 0.0 6 1065 24 2 1 0 0 1 148 1134 0.0 6 1103 28 0 1 1 1 149 1237 0.0 6 1205 21 3 4 3 0 1 150 1340 0.0 6 1314 21 1 2 1 0 1 151 1470 0.0 6 1434 29 2 3 1 0 1 152 1887 0.0 6 1845 35 2 0 1 1 3 153 1315 0.0 6 1289 18 1 2 1 2 2 154 1684 0.0 6 1642 34 1 1 4 1 1 155 1570 0.0 6 1546 15 5 0 1 2 1 156 1252 0.0 6 1230 17 2 1 2 157 1451 0.0 6 1421 24 0 0 2 2 2 158 1259 0.0 6 1237 18 0 1 3 159 1213 0.0 6 1195 15 1 0 1 0 1 160 1259 0.0 6 1229 24 2 0 0 2 2 161 1088 0.0 6 1069 18 0 0 0 0 1 162 1104 0.0 6 1081 20 2 0 1 163 975 0.0 6 955 13 3 1 0 3 164 950 0.0 6 935 9 1 2 1 2 165 896 0.0 6 879 14 1 0 1 1 166 917 0.0 6 892 21 0 0 0 1 3 167 801 0.0 6 790 10 0 1 168 1024 0.0 6 1015 8 0 1 169 823 0.0 6 806 13 1 1 0 1 1 170 1023 0.0 6 1006 14 0 1 0 2 171 883 0.0 6 870 9 2 0 1 1 172 800 0.0 6 785 13 1 0 1 173 880 0.0 6 870 7 0 0 2 1 174 952 0.0 6 934 15 2 0 1 175 916 0.0 6 902 11 1 0 1 0 1 176 826 0.0 6 809 14 2 0 0 0 1 177 934 0.0 6 916 18 178 801 0.0 6 784 13 2 0 1 0 1 179 768 0.0 6 755 11 0 2 180 688 0.0 6 681 4 0 0 2 1 181 572 0.0 6 559 11 1 1 182 486 0.0 6 482 4 183 522 0.0 6 515 6 1 184 476 0.0 6 466 9 0 0 0 0 1 185 768 0.0 6 760 6 1 1 186 978 0.0 6 969 9 187 735 0.0 6 722 11 1 0 1 188 582 0.0 6 574 7 0 0 1 189 526 0.0 6 515 9 1 1 190 544 0.0 6 536 8 191 505 0.0 6 496 9 192 424 0.0 6 417 7 193 457 0.0 6 442 10 0 3 1 1 194 402 0.0 6 397 4 1 195 406 0.0 6 399 7 196 389 0.0 6 379 8 2 197 251 0.0 6 247 3 0 0 0 1 198 437 0.0 6 428 9 199 412 0.0 6 395 14 0 0 1 2 200 312 0.0 6 309 3 201 286 0.0 6 278 7 0 1 202 264 0.0 6 258 5 1 203 212 0.0 6 209 3 204 220 0.0 6 216 3 0 0 0 0 1 205 168 0.0 6 164 3 0 0 0 1 206 184 0.0 6 180 3 1 207 134 0.0 6 133 1 208 117 0.0 6 116 1 209 147 0.0 6 147 210 125 0.0 6 120 5 211 117 0.0 6 117 212 85 0.0 6 81 4 213 87 0.0 6 85 2 214 94 0.0 6 90 3 0 1 215 105 0.0 6 104 1 216 246 0.0 6 239 5 1 0 0 1 217 116 0.0 6 110 6 218 127 0.0 6 120 7 219 103 0.0 6 103 220 86 0.0 6 84 2 221 69 0.0 6 67 2 222 72 0.0 6 72 223 119 0.0 6 115 4 224 53 0.0 6 52 1 225 45 0.0 6 44 1 226 40 0.0 6 39 1 227 35 0.0 6 35 228 25 0.0 6 24 1 229 28 0.0 6 27 1 230 29 0.0 6 27 2 231 28 0.0 6 27 1 232 33 0.0 6 33 233 20 0.0 6 20 234 18 0.0 6 18 235 7 0.0 6 7 236 7 0.0 6 7 237 4 0.0 6 4 238 7 0.0 6 7 239 3 0.0 6 3 240 8 0.0 6 8 241 12 0.0 6 12 242 12 0.0 6 12 243 15 0.0 6 13 1 0 0 0 0 1 244 10 0.0 6 10 245 3 0.0 6 3 246 5 0.0 6 5 247 3 0.0 6 3 249 3 0.0 6 3 250 4 0.0 6 4 251 1 0.0 6 1 252 3 0.0 6 3 253 1 0.0 6 1 254 1 0.0 6 1 279 3 0.0 6 3 280 16 0.0 6 16 281 124 0.0 6 124 282 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1037 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 50 2 0.0 6 2 51 3 0.0 6 3 56 1 0.0 6 1 59 6 0.0 6 5 0 0 1 63 2 0.0 6 2 67 1 0.0 6 1 69 4 0.0 6 4 70 8 0.0 6 8 71 20 0.0 6 20 72 25 0.0 6 25 73 6 0.0 6 6 74 7 0.0 6 7 75 14 0.0 6 14 76 11 0.0 6 10 1 77 9 0.0 6 9 78 22 0.0 6 22 79 6 0.0 6 6 80 9 0.0 6 9 81 8 0.0 6 8 82 8 0.0 6 8 83 10 0.0 6 10 84 7 0.0 6 7 85 10 0.0 6 10 86 12 0.0 6 12 87 9 0.0 6 9 88 9 0.0 6 9 89 9 0.0 6 9 90 10 0.0 6 10 91 7 0.0 6 7 92 8 0.0 6 8 93 12 0.0 6 12 94 3 0.0 6 3 95 9 0.0 6 9 96 10 0.0 6 9 0 0 1 97 5 0.0 6 5 98 7 0.0 6 7 99 7 0.0 6 7 100 1 0.0 6 1 101 1 0.0 6 1 102 5 0.0 6 5 103 7 0.0 6 7 104 7 0.0 6 7 105 7 0.0 6 7 106 7 0.0 6 7 107 12 0.0 6 12 108 36 0.0 6 36 109 7 0.0 6 7 110 6 0.0 6 6 111 4 0.0 6 4 112 7 0.0 6 6 0 0 1 113 1 0.0 6 1 114 5 0.0 6 5 115 11 0.0 6 11 116 7 0.0 6 7 117 8 0.0 6 8 118 6 0.0 6 6 119 4 0.0 6 4 120 2 0.0 6 2 121 10 0.0 6 10 122 9 0.0 6 9 123 9 0.0 6 9 124 6 0.0 6 6 125 9 0.0 6 9 126 12 0.0 6 12 127 4 0.0 6 4 128 5 0.0 6 5 129 5 0.0 6 5 130 6 0.0 6 6 131 5 0.0 6 5 132 8 0.0 6 7 1 133 2 0.0 6 2 134 13 0.0 6 13 135 6 0.0 6 6 136 7 0.0 6 7 137 9 0.0 6 9 138 10 0.0 6 10 139 6 0.0 6 6 140 8 0.0 6 8 141 14 0.0 6 13 1 142 4 0.0 6 4 143 12 0.0 6 12 144 4 0.0 6 4 145 3 0.0 6 3 146 5 0.0 6 5 147 7 0.0 6 7 148 2 0.0 6 2 149 9 0.0 6 9 150 7 0.0 6 7 151 4 0.0 6 4 152 7 0.0 6 7 153 8 0.0 6 7 0 0 0 1 154 4 0.0 6 4 155 4 0.0 6 4 156 4 0.0 6 4 157 7 0.0 6 7 158 4 0.0 6 4 159 6 0.0 6 6 160 4 0.0 6 4 161 9 0.0 6 9 162 10 0.0 6 10 163 8 0.0 6 7 0 0 0 1 164 15 0.0 6 15 165 4 0.0 6 4 166 3 0.0 6 3 167 11 0.0 6 11 168 5 0.0 6 5 169 2 0.0 6 2 170 6 0.0 6 6 171 7 0.0 6 7 172 14 0.0 6 14 173 5 0.0 6 5 174 5 0.0 6 5 175 9 0.0 6 9 176 6 0.0 6 6 177 8 0.0 6 8 178 6 0.0 6 5 0 0 0 0 0 1 179 8 0.0 6 8 180 10 0.0 6 10 181 3 0.0 6 3 182 5 0.0 6 5 183 4 0.0 6 3 1 184 4 0.0 6 3 0 0 0 1 185 6 0.0 6 6 186 9 0.0 6 9 187 9 0.0 6 9 188 8 0.0 6 8 189 2 0.0 6 2 190 4 0.0 6 4 191 6 0.0 6 6 192 6 0.0 6 6 193 5 0.0 6 5 194 2 0.0 6 2 195 5 0.0 6 4 1 196 4 0.0 6 4 197 6 0.0 6 6 198 5 0.0 6 5 199 1 0.0 6 1 200 3 0.0 6 3 201 10 0.0 6 10 202 7 0.0 6 7 203 4 0.0 6 4 204 2 0.0 6 2 205 3 0.0 6 3 206 2 0.0 6 2 208 2 0.0 6 2 210 4 0.0 6 3 1 212 1 0.0 6 1 213 1 0.0 6 1 215 4 0.0 6 4 217 3 0.0 6 3 218 1 0.0 6 1 219 2 0.0 6 2 220 1 0.0 6 1 222 1 0.0 6 1 223 1 0.0 6 1 227 1 0.0 6 1 229 1 0.0 6 1 233 1 0.0 6 0 1 234 1 0.0 6 1 236 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_02_25_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_02_252_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_02_25_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_02_252_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.98 s (27 µs/read; 2.26 M reads/minute). === Summary === Total read pairs processed: 74,508 Read 1 with adapter: 74,498 (100.0%) Read 2 with adapter: 74,508 (100.0%) == Read fate breakdown == Pairs that were too short: 38 (0.1%) Pairs discarded as untrimmed: 10 (0.0%) Pairs written (passing filters): 74,460 (99.9%) Total basepairs processed: 31,909,757 bp Read 1: 18,011,044 bp Read 2: 13,898,713 bp Total written (filtered): 4,861,697 bp (15.2%) Read 1: 2,525,934 bp Read 2: 2,335,763 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 72840 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1164.2 0 0 2 4 4 291.0 1 4 6 4 18.2 1 1 0 3 7 4 4.5 2 1 0 3 10 1 0.1 3 1 13 2 0.0 3 2 14 4 0.0 4 3 1 15 1 0.0 4 1 16 1 0.0 4 1 17 2 0.0 5 2 18 3 0.0 5 2 1 19 3 0.0 5 1 2 20 93 0.0 6 91 2 21 38 0.0 6 38 22 42 0.0 6 38 3 1 23 31 0.0 6 31 24 30 0.0 6 27 3 25 23 0.0 6 21 2 26 19 0.0 6 18 0 0 0 0 1 27 7 0.0 6 7 28 15 0.0 6 15 29 6 0.0 6 6 30 10 0.0 6 9 1 31 8 0.0 6 8 32 4 0.0 6 3 1 33 8 0.0 6 8 34 3 0.0 6 3 35 11 0.0 6 10 0 1 36 7 0.0 6 7 37 6 0.0 6 5 0 1 38 4 0.0 6 4 39 10 0.0 6 9 1 40 9 0.0 6 9 41 10 0.0 6 10 42 7 0.0 6 7 43 6 0.0 6 6 44 7 0.0 6 7 45 11 0.0 6 10 1 46 6 0.0 6 6 47 9 0.0 6 9 48 14 0.0 6 14 49 8 0.0 6 8 50 5 0.0 6 5 51 5 0.0 6 4 1 52 11 0.0 6 11 53 6 0.0 6 5 1 54 4 0.0 6 4 55 7 0.0 6 7 56 5 0.0 6 5 57 6 0.0 6 6 58 7 0.0 6 6 0 0 0 0 1 59 3 0.0 6 3 60 7 0.0 6 7 61 9 0.0 6 9 62 5 0.0 6 3 2 63 12 0.0 6 12 64 6 0.0 6 6 65 7 0.0 6 7 66 8 0.0 6 8 67 6 0.0 6 5 1 68 10 0.0 6 10 69 9 0.0 6 7 2 70 10 0.0 6 10 71 13 0.0 6 13 72 12 0.0 6 10 1 1 73 9 0.0 6 8 1 74 10 0.0 6 10 75 16 0.0 6 14 2 76 7 0.0 6 7 77 7 0.0 6 7 78 7 0.0 6 7 79 12 0.0 6 12 80 13 0.0 6 13 81 9 0.0 6 8 1 82 14 0.0 6 13 1 83 12 0.0 6 12 84 14 0.0 6 14 85 10 0.0 6 10 86 10 0.0 6 8 1 0 0 0 1 87 10 0.0 6 9 1 88 12 0.0 6 11 0 0 0 0 1 89 10 0.0 6 10 90 7 0.0 6 5 2 91 11 0.0 6 11 92 12 0.0 6 11 1 93 11 0.0 6 11 94 16 0.0 6 15 1 95 12 0.0 6 10 2 96 12 0.0 6 12 97 9 0.0 6 9 98 16 0.0 6 15 0 0 0 0 0 1 99 16 0.0 6 14 2 100 16 0.0 6 15 1 101 17 0.0 6 16 1 102 20 0.0 6 19 1 103 27 0.0 6 26 0 0 0 0 0 1 104 32 0.0 6 32 105 15 0.0 6 15 106 24 0.0 6 23 1 107 19 0.0 6 17 1 1 108 14 0.0 6 14 109 28 0.0 6 28 110 23 0.0 6 22 1 111 14 0.0 6 14 112 26 0.0 6 25 1 113 15 0.0 6 15 114 19 0.0 6 18 1 115 28 0.0 6 28 116 23 0.0 6 22 1 117 23 0.0 6 23 118 33 0.0 6 32 1 119 22 0.0 6 18 2 0 0 0 1 1 120 25 0.0 6 25 121 36 0.0 6 35 1 122 21 0.0 6 21 123 23 0.0 6 23 124 20 0.0 6 20 125 40 0.0 6 40 126 26 0.0 6 26 127 28 0.0 6 28 128 29 0.0 6 27 2 129 38 0.0 6 38 130 35 0.0 6 34 1 131 34 0.0 6 34 132 49 0.0 6 48 1 133 42 0.0 6 42 134 47 0.0 6 46 1 135 52 0.0 6 51 1 136 42 0.0 6 42 137 58 0.0 6 58 138 55 0.0 6 53 2 139 69 0.0 6 69 140 57 0.0 6 57 141 65 0.0 6 65 142 164 0.0 6 164 143 343 0.0 6 342 1 144 426 0.0 6 424 2 145 246 0.0 6 245 1 146 285 0.0 6 285 147 401 0.0 6 399 2 148 310 0.0 6 310 149 253 0.0 6 253 150 354 0.0 6 351 3 151 269 0.0 6 268 1 152 240 0.0 6 240 153 278 0.0 6 275 1 0 0 0 2 154 243 0.0 6 242 1 155 275 0.0 6 271 2 1 0 0 1 156 255 0.0 6 255 157 233 0.0 6 233 158 218 0.0 6 216 1 0 0 0 1 159 275 0.0 6 274 1 160 267 0.0 6 264 2 0 0 0 1 161 317 0.0 6 316 1 162 301 0.0 6 301 163 314 0.0 6 308 6 164 352 0.0 6 349 3 165 346 0.0 6 344 2 166 228 0.0 6 225 2 0 0 0 1 167 269 0.0 6 268 1 168 329 0.0 6 328 1 169 257 0.0 6 256 1 170 328 0.0 6 324 4 171 323 0.0 6 318 4 0 0 1 172 304 0.0 6 302 2 173 323 0.0 6 321 2 174 426 0.0 6 423 2 0 0 0 0 1 175 379 0.0 6 373 6 176 329 0.0 6 324 4 0 0 0 0 1 177 381 0.0 6 378 2 1 178 343 0.0 6 341 1 1 179 383 0.0 6 380 2 0 1 180 925 0.0 6 921 4 181 480 0.0 6 476 3 0 1 182 391 0.0 6 390 1 183 480 0.0 6 476 3 0 0 0 1 184 421 0.0 6 418 3 185 400 0.0 6 397 1 1 0 0 1 186 495 0.0 6 489 6 187 423 0.0 6 420 3 188 481 0.0 6 478 2 0 0 0 0 1 189 552 0.0 6 549 3 190 492 0.0 6 488 4 191 527 0.0 6 524 2 0 0 0 1 192 627 0.0 6 626 1 193 661 0.0 6 654 6 0 0 0 1 194 761 0.0 6 752 8 0 0 0 1 195 893 0.0 6 889 1 1 0 0 1 1 196 936 0.0 6 930 6 197 989 0.0 6 986 3 198 808 0.0 6 803 5 199 676 0.0 6 672 3 0 0 1 200 704 0.0 6 696 8 201 703 0.0 6 701 2 202 795 0.0 6 787 8 203 751 0.0 6 745 6 204 1079 0.0 6 1071 7 1 205 1033 0.0 6 1026 6 0 1 206 838 0.0 6 833 3 0 0 1 0 1 207 914 0.0 6 909 4 0 0 0 0 1 208 648 0.0 6 645 1 1 0 0 1 209 801 0.0 6 793 7 0 0 0 0 1 210 926 0.0 6 923 3 211 1053 0.0 6 1049 4 212 931 0.0 6 922 5 2 1 0 1 213 1120 0.0 6 1115 4 1 214 1201 0.0 6 1193 5 2 0 1 215 961 0.0 6 954 6 0 0 1 216 977 0.0 6 969 5 2 1 217 1002 0.0 6 994 8 218 921 0.0 6 914 7 219 727 0.0 6 724 2 0 0 0 1 220 764 0.0 6 756 7 0 0 0 1 221 818 0.0 6 815 2 0 0 0 0 1 222 888 0.0 6 886 1 1 223 849 0.0 6 844 2 1 1 0 0 1 224 905 0.0 6 902 3 225 934 0.0 6 929 3 2 226 785 0.0 6 781 3 0 0 0 1 227 697 0.0 6 691 6 228 804 0.0 6 798 4 0 1 0 0 1 229 707 0.0 6 705 2 230 672 0.0 6 669 3 231 709 0.0 6 699 8 0 1 0 1 232 720 0.0 6 715 5 233 633 0.0 6 628 5 234 537 0.0 6 532 3 1 0 0 1 235 621 0.0 6 618 2 0 0 0 1 236 605 0.0 6 603 2 237 643 0.0 6 634 8 1 238 563 0.0 6 560 3 239 586 0.0 6 584 0 1 0 0 1 240 568 0.0 6 563 5 241 534 0.0 6 530 3 1 242 481 0.0 6 475 6 243 506 0.0 6 501 5 244 394 0.0 6 391 3 245 379 0.0 6 379 246 339 0.0 6 337 2 247 346 0.0 6 345 1 248 321 0.0 6 318 2 0 1 249 333 0.0 6 332 1 250 293 0.0 6 289 4 251 268 0.0 6 267 1 252 287 0.0 6 284 3 253 237 0.0 6 235 2 254 273 0.0 6 268 5 255 261 0.0 6 261 256 218 0.0 6 218 257 245 0.0 6 244 1 258 308 0.0 6 305 3 259 236 0.0 6 232 4 260 197 0.0 6 196 1 261 210 0.0 6 209 1 262 179 0.0 6 177 2 263 194 0.0 6 192 2 264 151 0.0 6 151 265 148 0.0 6 147 1 266 141 0.0 6 140 1 267 183 0.0 6 180 3 268 160 0.0 6 159 1 269 148 0.0 6 148 270 147 0.0 6 144 3 271 117 0.0 6 117 272 177 0.0 6 176 1 273 196 0.0 6 189 7 274 220 0.0 6 217 2 0 0 0 1 275 263 0.0 6 263 276 306 0.0 6 305 1 277 992 0.0 6 984 7 0 0 0 1 278 3005 0.0 6 2955 48 2 279 69 0.0 6 66 3 280 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1658 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 2 0.0 6 0 0 2 63 1 0.0 6 0 1 64 53 0.0 6 50 3 72 1 0.0 6 1 73 7 0.0 6 7 74 14 0.0 6 14 75 9 0.0 6 9 76 9 0.0 6 9 77 11 0.0 6 11 78 11 0.0 6 11 79 4 0.0 6 4 80 6 0.0 6 6 81 8 0.0 6 8 83 8 0.0 6 8 84 6 0.0 6 6 85 5 0.0 6 5 86 3 0.0 6 3 87 5 0.0 6 5 88 4 0.0 6 4 89 3 0.0 6 3 90 9 0.0 6 9 91 2 0.0 6 2 92 6 0.0 6 6 93 3 0.0 6 3 94 7 0.0 6 7 95 5 0.0 6 5 96 7 0.0 6 7 97 3 0.0 6 3 98 8 0.0 6 8 99 11 0.0 6 11 100 7 0.0 6 7 101 2 0.0 6 2 102 7 0.0 6 6 1 103 6 0.0 6 6 104 3 0.0 6 3 105 7 0.0 6 7 106 4 0.0 6 4 107 4 0.0 6 4 108 9 0.0 6 8 1 109 1 0.0 6 1 110 19 0.0 6 19 111 21 0.0 6 21 112 4 0.0 6 4 113 8 0.0 6 8 114 9 0.0 6 9 115 6 0.0 6 6 116 4 0.0 6 4 117 6 0.0 6 6 118 3 0.0 6 3 119 7 0.0 6 7 120 9 0.0 6 9 121 4 0.0 6 4 122 9 0.0 6 9 123 5 0.0 6 5 124 5 0.0 6 5 125 9 0.0 6 9 126 5 0.0 6 5 127 9 0.0 6 9 128 7 0.0 6 7 129 6 0.0 6 6 130 11 0.0 6 11 131 5 0.0 6 5 132 10 0.0 6 10 133 13 0.0 6 13 134 17 0.0 6 17 135 10 0.0 6 10 136 9 0.0 6 9 137 8 0.0 6 8 138 10 0.0 6 10 139 8 0.0 6 8 140 9 0.0 6 9 141 10 0.0 6 10 142 13 0.0 6 13 143 8 0.0 6 8 144 6 0.0 6 6 145 11 0.0 6 11 146 7 0.0 6 7 147 10 0.0 6 10 148 11 0.0 6 11 149 6 0.0 6 6 150 9 0.0 6 9 151 3 0.0 6 3 152 9 0.0 6 9 153 15 0.0 6 15 154 7 0.0 6 7 155 19 0.0 6 18 1 156 15 0.0 6 15 157 5 0.0 6 5 158 14 0.0 6 14 159 12 0.0 6 12 160 12 0.0 6 12 161 8 0.0 6 8 162 16 0.0 6 16 163 9 0.0 6 9 164 9 0.0 6 9 165 15 0.0 6 15 166 13 0.0 6 13 167 14 0.0 6 14 168 13 0.0 6 13 169 12 0.0 6 12 170 13 0.0 6 13 171 18 0.0 6 18 172 14 0.0 6 14 173 13 0.0 6 13 174 12 0.0 6 12 175 10 0.0 6 10 176 9 0.0 6 9 177 13 0.0 6 13 178 12 0.0 6 12 179 10 0.0 6 10 180 8 0.0 6 8 181 6 0.0 6 6 182 6 0.0 6 6 183 7 0.0 6 7 184 12 0.0 6 12 185 16 0.0 6 16 186 13 0.0 6 13 187 17 0.0 6 16 1 188 8 0.0 6 8 189 17 0.0 6 17 190 11 0.0 6 11 191 11 0.0 6 11 192 24 0.0 6 24 193 9 0.0 6 9 194 14 0.0 6 14 195 14 0.0 6 14 196 14 0.0 6 14 197 13 0.0 6 13 198 10 0.0 6 10 199 12 0.0 6 12 200 9 0.0 6 9 201 9 0.0 6 9 202 9 0.0 6 9 203 15 0.0 6 15 204 12 0.0 6 12 205 7 0.0 6 7 206 9 0.0 6 9 207 13 0.0 6 13 208 14 0.0 6 14 209 12 0.0 6 12 210 4 0.0 6 4 211 7 0.0 6 7 212 9 0.0 6 9 213 11 0.0 6 11 214 13 0.0 6 13 215 11 0.0 6 11 216 4 0.0 6 4 217 4 0.0 6 4 218 8 0.0 6 8 219 7 0.0 6 7 220 5 0.0 6 5 221 8 0.0 6 8 222 9 0.0 6 9 223 8 0.0 6 8 224 11 0.0 6 11 225 4 0.0 6 4 226 4 0.0 6 4 227 4 0.0 6 4 228 12 0.0 6 12 229 12 0.0 6 12 230 13 0.0 6 13 231 11 0.0 6 11 232 7 0.0 6 7 233 9 0.0 6 9 234 4 0.0 6 4 235 7 0.0 6 7 236 5 0.0 6 5 237 6 0.0 6 6 238 4 0.0 6 4 239 5 0.0 6 5 240 2 0.0 6 2 241 5 0.0 6 5 242 1 0.0 6 1 243 4 0.0 6 4 244 3 0.0 6 3 245 4 0.0 6 4 246 1 0.0 6 1 247 2 0.0 6 2 248 4 0.0 6 4 249 7 0.0 6 7 250 4 0.0 6 4 251 2 0.0 6 2 252 2 0.0 6 2 253 2 0.0 6 2 254 3 0.0 6 3 255 5 0.0 6 5 256 3 0.0 6 3 257 2 0.0 6 2 258 6 0.0 6 6 260 2 0.0 6 2 261 1 0.0 6 1 262 3 0.0 6 3 264 2 0.0 6 2 265 2 0.0 6 2 266 2 0.0 6 2 267 2 0.0 6 2 270 1 0.0 6 1 271 3 0.0 6 3 273 1 0.0 6 1 274 2 0.0 6 2 275 1 0.0 6 1 276 1 0.0 6 1 282 2 0.0 6 2 283 1 0.0 6 1 284 3 0.0 6 3 285 1 0.0 6 1 287 1 0.0 6 1 288 2 0.0 6 2 289 1 0.0 6 1 290 2 0.0 6 1 1 299 1 0.0 6 1 308 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 73260 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1164.2 0 1 7 1 4.5 2 1 10 2 0.1 3 1 1 11 4 0.0 3 4 12 8 0.0 3 8 13 13 0.0 3 11 2 14 15 0.0 4 15 15 1 0.0 4 0 1 16 12 0.0 4 9 3 17 48 0.0 5 47 1 18 34 0.0 5 25 8 1 19 29 0.0 5 27 1 1 20 87 0.0 6 82 5 21 48 0.0 6 46 2 22 24 0.0 6 21 2 1 23 24 0.0 6 24 24 23 0.0 6 21 1 0 0 1 25 19 0.0 6 18 1 26 20 0.0 6 20 27 19 0.0 6 15 2 1 0 0 1 28 9 0.0 6 9 29 14 0.0 6 13 1 30 18 0.0 6 17 1 31 23 0.0 6 21 2 32 18 0.0 6 16 1 0 0 0 1 33 7 0.0 6 7 34 11 0.0 6 9 1 0 1 35 20 0.0 6 17 3 36 10 0.0 6 9 0 1 37 15 0.0 6 13 2 38 16 0.0 6 13 3 39 13 0.0 6 12 1 40 17 0.0 6 17 41 13 0.0 6 13 42 12 0.0 6 11 1 43 15 0.0 6 14 1 44 17 0.0 6 15 2 45 11 0.0 6 11 46 20 0.0 6 18 1 0 0 0 1 47 17 0.0 6 16 1 48 19 0.0 6 19 49 23 0.0 6 20 2 1 50 17 0.0 6 16 1 51 18 0.0 6 15 3 52 37 0.0 6 35 2 53 27 0.0 6 25 2 54 29 0.0 6 28 1 55 30 0.0 6 29 1 56 18 0.0 6 17 0 0 0 0 0 1 57 21 0.0 6 17 2 1 0 1 58 23 0.0 6 20 3 59 30 0.0 6 26 2 0 2 60 31 0.0 6 30 1 61 40 0.0 6 37 3 62 26 0.0 6 26 63 29 0.0 6 23 4 0 1 0 1 64 35 0.0 6 34 1 65 38 0.0 6 34 4 66 34 0.0 6 33 1 67 68 0.0 6 63 4 1 68 174 0.0 6 169 4 1 69 79 0.0 6 72 5 0 0 0 1 1 70 70 0.0 6 68 2 71 62 0.0 6 57 3 0 0 1 0 1 72 61 0.0 6 57 3 0 1 73 47 0.0 6 43 4 74 53 0.0 6 49 3 0 0 0 1 75 58 0.0 6 55 3 76 66 0.0 6 61 4 0 0 0 0 1 77 44 0.0 6 42 2 78 56 0.0 6 53 1 1 0 1 79 54 0.0 6 46 5 1 1 0 1 80 75 0.0 6 71 3 0 1 81 60 0.0 6 57 1 1 0 1 82 70 0.0 6 65 2 0 0 1 2 83 85 0.0 6 80 4 0 0 0 1 84 82 0.0 6 76 4 1 0 1 85 53 0.0 6 51 2 86 64 0.0 6 61 2 0 0 1 87 61 0.0 6 56 5 88 75 0.0 6 71 3 0 0 0 0 1 89 71 0.0 6 66 3 1 0 0 1 90 73 0.0 6 69 2 1 0 1 91 90 0.0 6 85 4 0 0 0 0 1 92 74 0.0 6 73 1 93 97 0.0 6 91 5 0 1 94 103 0.0 6 90 13 95 108 0.0 6 102 6 96 99 0.0 6 95 2 0 1 1 97 98 0.0 6 96 2 98 112 0.0 6 107 2 1 1 1 99 121 0.0 6 117 4 100 95 0.0 6 93 2 101 120 0.0 6 116 2 1 0 0 1 102 120 0.0 6 117 3 103 130 0.0 6 128 2 104 164 0.0 6 160 4 105 249 0.0 6 236 9 2 1 0 0 1 106 155 0.0 6 149 6 107 147 0.0 6 146 0 0 1 108 138 0.0 6 132 6 109 144 0.0 6 140 4 110 159 0.0 6 153 5 0 1 111 168 0.0 6 155 10 2 1 112 181 0.0 6 172 7 1 0 0 0 1 113 207 0.0 6 200 7 114 194 0.0 6 191 2 0 0 1 115 209 0.0 6 207 1 0 0 0 1 116 211 0.0 6 208 2 0 0 0 1 117 203 0.0 6 199 3 1 118 249 0.0 6 243 5 0 0 0 0 1 119 236 0.0 6 231 4 0 1 120 201 0.0 6 196 3 0 2 121 223 0.0 6 215 5 1 1 1 122 240 0.0 6 237 3 123 237 0.0 6 225 10 1 0 1 124 293 0.0 6 283 8 0 0 0 1 1 125 293 0.0 6 285 8 126 340 0.0 6 335 4 0 0 0 0 1 127 336 0.0 6 328 6 0 0 2 128 361 0.0 6 358 1 0 1 0 0 1 129 385 0.0 6 376 5 1 0 1 0 2 130 427 0.0 6 417 8 0 0 2 131 473 0.0 6 459 13 1 132 492 0.0 6 476 14 0 1 0 1 133 524 0.0 6 514 5 4 0 0 0 1 134 568 0.0 6 554 11 1 1 0 1 135 555 0.0 6 544 9 0 1 0 0 1 136 541 0.0 6 520 17 2 0 0 0 2 137 556 0.0 6 547 6 3 138 541 0.0 6 530 9 0 0 0 2 139 542 0.0 6 523 17 2 140 614 0.0 6 598 12 2 1 1 141 753 0.0 6 733 15 0 2 1 2 142 1001 0.0 6 984 15 1 0 0 0 1 143 1036 0.0 6 1019 16 0 0 0 0 1 144 917 0.0 6 900 10 0 0 3 1 3 145 983 0.0 6 961 17 1 0 2 1 1 146 1099 0.0 6 1084 13 1 0 1 147 1088 0.0 6 1071 14 2 0 0 1 148 1093 0.0 6 1075 16 1 1 149 1319 0.0 6 1292 23 1 0 1 1 1 150 1305 0.0 6 1284 18 2 0 0 0 1 151 1794 0.0 6 1762 26 1 2 2 1 152 1402 0.0 6 1373 20 2 2 1 3 1 153 1523 0.0 6 1495 24 0 0 2 1 1 154 1492 0.0 6 1466 19 1 0 3 0 3 155 1242 0.0 6 1216 20 1 1 1 1 2 156 1280 0.0 6 1266 11 0 0 2 0 1 157 1241 0.0 6 1218 17 1 2 2 1 158 1325 0.0 6 1302 20 0 0 0 3 159 1293 0.0 6 1276 13 2 1 1 160 1203 0.0 6 1180 18 0 1 2 0 2 161 1159 0.0 6 1149 10 162 1124 0.0 6 1111 9 1 2 0 1 163 989 0.0 6 978 9 0 0 0 0 2 164 1025 0.0 6 1008 14 0 1 2 165 964 0.0 6 954 9 0 1 166 940 0.0 6 928 9 1 1 1 167 1080 0.0 6 1065 13 1 0 1 168 934 0.0 6 923 10 0 1 169 1101 0.0 6 1089 11 0 1 170 921 0.0 6 913 8 171 919 0.0 6 910 8 0 0 1 172 1060 0.0 6 1054 6 173 1029 0.0 6 1016 10 1 1 0 1 174 1032 0.0 6 1021 11 175 948 0.0 6 942 3 0 1 1 0 1 176 912 0.0 6 901 11 177 970 0.0 6 961 9 178 791 0.0 6 780 11 179 859 0.0 6 855 3 0 1 180 631 0.0 6 627 4 181 588 0.0 6 579 7 0 1 0 1 182 539 0.0 6 537 2 183 575 0.0 6 573 2 184 760 0.0 6 753 6 0 1 185 1019 0.0 6 1012 4 0 1 0 0 2 186 864 0.0 6 855 7 0 1 0 1 187 639 0.0 6 631 8 188 541 0.0 6 527 11 0 1 0 2 189 544 0.0 6 540 4 190 542 0.0 6 537 5 191 525 0.0 6 520 5 192 506 0.0 6 498 7 0 0 1 193 347 0.0 6 345 2 194 398 0.0 6 393 4 0 0 1 195 345 0.0 6 341 4 196 358 0.0 6 353 5 197 372 0.0 6 366 6 198 310 0.0 6 305 3 1 1 199 395 0.0 6 389 6 200 272 0.0 6 269 2 0 1 201 256 0.0 6 254 2 202 204 0.0 6 199 4 1 203 225 0.0 6 224 1 204 168 0.0 6 165 3 205 129 0.0 6 125 4 206 153 0.0 6 152 1 207 119 0.0 6 118 1 208 127 0.0 6 126 1 209 117 0.0 6 115 2 210 109 0.0 6 108 1 211 97 0.0 6 95 2 212 101 0.0 6 99 2 213 102 0.0 6 99 2 1 214 88 0.0 6 87 1 215 107 0.0 6 106 0 0 1 216 92 0.0 6 90 2 217 79 0.0 6 77 2 218 57 0.0 6 56 1 219 51 0.0 6 51 220 52 0.0 6 52 221 78 0.0 6 78 222 62 0.0 6 62 223 67 0.0 6 67 224 24 0.0 6 24 225 36 0.0 6 35 1 226 21 0.0 6 21 227 18 0.0 6 17 1 228 16 0.0 6 15 1 229 12 0.0 6 12 230 6 0.0 6 6 231 9 0.0 6 7 2 232 26 0.0 6 25 1 233 29 0.0 6 28 1 234 6 0.0 6 6 235 4 0.0 6 4 236 3 0.0 6 3 237 4 0.0 6 4 238 2 0.0 6 2 239 5 0.0 6 4 1 240 10 0.0 6 10 241 5 0.0 6 5 242 8 0.0 6 8 243 8 0.0 6 8 244 6 0.0 6 6 245 2 0.0 6 2 246 5 0.0 6 5 247 2 0.0 6 2 248 1 0.0 6 1 249 2 0.0 6 2 251 1 0.0 6 1 277 1 0.0 6 1 278 1 0.0 6 1 279 12 0.0 6 12 280 81 0.0 6 79 1 1 281 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1248 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 3 84 1164.2 0 84 47 1 0.0 6 0 0 1 48 2 0.0 6 0 2 52 1 0.0 6 1 55 1 0.0 6 1 56 1 0.0 6 1 57 1 0.0 6 1 70 2 0.0 6 2 71 3 0.0 6 3 72 9 0.0 6 9 73 9 0.0 6 8 1 74 11 0.0 6 11 75 9 0.0 6 9 76 17 0.0 6 17 77 10 0.0 6 10 78 8 0.0 6 8 79 16 0.0 6 16 80 10 0.0 6 10 81 9 0.0 6 9 82 7 0.0 6 6 1 83 2 0.0 6 1 0 0 0 1 84 7 0.0 6 7 85 6 0.0 6 6 86 5 0.0 6 5 87 3 0.0 6 3 88 9 0.0 6 9 89 4 0.0 6 4 90 12 0.0 6 12 91 9 0.0 6 9 92 7 0.0 6 7 93 9 0.0 6 9 94 5 0.0 6 5 95 3 0.0 6 3 96 3 0.0 6 3 97 8 0.0 6 8 98 4 0.0 6 4 99 8 0.0 6 8 100 6 0.0 6 6 101 4 0.0 6 4 102 9 0.0 6 9 103 7 0.0 6 7 104 5 0.0 6 5 105 4 0.0 6 4 106 14 0.0 6 14 107 3 0.0 6 3 108 11 0.0 6 11 109 31 0.0 6 31 110 9 0.0 6 9 111 6 0.0 6 6 112 10 0.0 6 10 113 1 0.0 6 1 114 3 0.0 6 3 115 11 0.0 6 11 116 9 0.0 6 9 117 9 0.0 6 9 118 8 0.0 6 8 119 6 0.0 6 6 120 5 0.0 6 5 121 8 0.0 6 8 122 4 0.0 6 4 123 10 0.0 6 10 124 13 0.0 6 13 125 5 0.0 6 5 126 11 0.0 6 11 127 10 0.0 6 10 128 5 0.0 6 5 129 1 0.0 6 1 130 7 0.0 6 7 131 16 0.0 6 16 132 13 0.0 6 13 133 17 0.0 6 16 1 134 3 0.0 6 3 135 11 0.0 6 11 136 7 0.0 6 7 137 7 0.0 6 6 0 0 0 1 138 5 0.0 6 5 139 9 0.0 6 9 140 1 0.0 6 1 141 6 0.0 6 6 142 7 0.0 6 7 143 5 0.0 6 5 144 12 0.0 6 12 145 14 0.0 6 14 146 7 0.0 6 7 147 9 0.0 6 9 148 9 0.0 6 9 149 6 0.0 6 6 150 15 0.0 6 15 151 10 0.0 6 10 152 13 0.0 6 13 153 14 0.0 6 14 154 15 0.0 6 15 155 8 0.0 6 8 156 6 0.0 6 6 157 18 0.0 6 18 158 8 0.0 6 8 159 17 0.0 6 17 160 13 0.0 6 13 161 10 0.0 6 10 162 11 0.0 6 11 163 13 0.0 6 13 164 10 0.0 6 9 0 0 0 1 165 10 0.0 6 10 166 22 0.0 6 20 0 0 0 2 167 10 0.0 6 10 168 11 0.0 6 11 169 12 0.0 6 12 170 5 0.0 6 5 171 17 0.0 6 16 0 0 1 172 11 0.0 6 10 1 173 16 0.0 6 16 174 14 0.0 6 14 175 9 0.0 6 9 176 13 0.0 6 12 1 177 7 0.0 6 7 178 7 0.0 6 7 179 12 0.0 6 12 180 7 0.0 6 7 181 12 0.0 6 11 1 182 6 0.0 6 6 183 9 0.0 6 9 184 5 0.0 6 5 185 4 0.0 6 4 186 4 0.0 6 4 187 12 0.0 6 12 188 6 0.0 6 5 1 189 10 0.0 6 10 190 9 0.0 6 9 191 9 0.0 6 8 0 0 0 1 192 2 0.0 6 2 193 1 0.0 6 1 194 3 0.0 6 3 195 2 0.0 6 2 196 7 0.0 6 6 0 0 1 197 3 0.0 6 3 198 3 0.0 6 3 199 3 0.0 6 3 200 6 0.0 6 6 201 2 0.0 6 2 202 5 0.0 6 5 203 1 0.0 6 1 204 4 0.0 6 4 205 5 0.0 6 5 206 3 0.0 6 3 207 1 0.0 6 1 209 1 0.0 6 1 210 2 0.0 6 2 212 1 0.0 6 1 213 2 0.0 6 2 214 1 0.0 6 1 215 2 0.0 6 2 216 1 0.0 6 1 217 1 0.0 6 1 219 1 0.0 6 1 220 1 0.0 6 1 222 3 0.0 6 3 224 1 0.0 6 1 225 1 0.0 6 1 229 1 0.0 6 1 230 1 0.0 6 1 234 1 0.0 6 1 236 1 0.0 6 1 245 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_03_26_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_03_253_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_03_26_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_03_253_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.27 s (25 µs/read; 2.37 M reads/minute). === Summary === Total read pairs processed: 89,761 Read 1 with adapter: 89,750 (100.0%) Read 2 with adapter: 89,700 (99.9%) == Read fate breakdown == Pairs that were too short: 6 (0.0%) Pairs discarded as untrimmed: 71 (0.1%) Pairs written (passing filters): 89,684 (99.9%) Total basepairs processed: 44,182,673 bp Read 1: 25,079,318 bp Read 2: 19,103,355 bp Total written (filtered): 5,859,629 bp (13.3%) Read 1: 2,979,964 bp Read 2: 2,879,665 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 89275 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 350.6 1 1 7 3 5.5 2 0 0 3 8 1 1.4 2 1 9 2 0.3 2 1 1 11 1 0.0 3 1 14 1 0.0 4 1 16 1 0.0 4 1 17 2 0.0 5 2 19 1 0.0 5 1 20 61 0.0 6 59 2 21 13 0.0 6 13 22 20 0.0 6 20 23 13 0.0 6 12 1 24 8 0.0 6 8 25 27 0.0 6 24 2 0 1 26 12 0.0 6 12 27 8 0.0 6 8 28 6 0.0 6 5 1 29 7 0.0 6 7 30 4 0.0 6 4 31 2 0.0 6 2 32 2 0.0 6 1 0 1 33 10 0.0 6 10 34 5 0.0 6 5 35 1 0.0 6 1 36 5 0.0 6 4 1 37 3 0.0 6 3 38 5 0.0 6 4 1 39 6 0.0 6 5 1 40 3 0.0 6 3 41 2 0.0 6 2 42 5 0.0 6 5 43 3 0.0 6 2 0 0 0 0 1 44 9 0.0 6 7 1 0 0 0 0 1 45 10 0.0 6 10 46 7 0.0 6 7 47 3 0.0 6 2 1 48 3 0.0 6 3 49 5 0.0 6 3 2 50 5 0.0 6 4 1 51 2 0.0 6 2 52 2 0.0 6 2 53 8 0.0 6 8 54 7 0.0 6 5 1 0 1 55 1 0.0 6 0 1 56 9 0.0 6 9 57 6 0.0 6 5 1 58 5 0.0 6 5 59 3 0.0 6 3 61 2 0.0 6 2 62 9 0.0 6 7 1 0 0 0 0 1 63 2 0.0 6 2 64 7 0.0 6 6 1 65 6 0.0 6 5 1 66 4 0.0 6 3 1 67 6 0.0 6 5 0 0 1 68 6 0.0 6 6 69 6 0.0 6 6 70 2 0.0 6 2 72 4 0.0 6 4 73 3 0.0 6 2 1 74 5 0.0 6 5 75 1 0.0 6 1 76 1 0.0 6 1 77 3 0.0 6 2 1 78 3 0.0 6 3 79 4 0.0 6 4 80 3 0.0 6 3 81 5 0.0 6 5 82 3 0.0 6 3 83 5 0.0 6 5 84 8 0.0 6 7 1 85 3 0.0 6 3 86 11 0.0 6 11 87 6 0.0 6 6 88 4 0.0 6 4 89 10 0.0 6 9 1 90 4 0.0 6 4 91 2 0.0 6 2 92 4 0.0 6 4 93 4 0.0 6 4 94 5 0.0 6 5 95 5 0.0 6 5 96 2 0.0 6 2 97 7 0.0 6 7 98 9 0.0 6 8 1 99 5 0.0 6 4 0 1 100 7 0.0 6 7 101 16 0.0 6 14 1 1 102 11 0.0 6 10 1 103 15 0.0 6 15 104 10 0.0 6 10 105 12 0.0 6 11 1 106 16 0.0 6 12 3 0 0 1 107 15 0.0 6 15 108 20 0.0 6 18 1 1 109 11 0.0 6 10 1 110 10 0.0 6 8 2 111 18 0.0 6 17 1 112 20 0.0 6 19 1 113 18 0.0 6 16 2 114 13 0.0 6 12 0 1 115 10 0.0 6 9 1 116 13 0.0 6 12 1 117 14 0.0 6 13 1 118 9 0.0 6 8 1 119 18 0.0 6 17 1 120 7 0.0 6 7 121 15 0.0 6 15 122 22 0.0 6 18 4 123 28 0.0 6 27 1 124 16 0.0 6 14 1 1 125 18 0.0 6 17 1 126 14 0.0 6 14 127 12 0.0 6 12 128 20 0.0 6 20 129 21 0.0 6 18 3 130 23 0.0 6 21 2 131 15 0.0 6 13 2 132 28 0.0 6 26 2 133 30 0.0 6 30 134 28 0.0 6 28 135 29 0.0 6 29 136 29 0.0 6 29 137 27 0.0 6 27 138 24 0.0 6 22 1 0 0 0 1 139 22 0.0 6 22 140 16 0.0 6 16 141 29 0.0 6 28 1 142 43 0.0 6 43 143 67 0.0 6 65 2 144 112 0.0 6 109 2 0 1 145 152 0.0 6 150 1 0 1 146 115 0.0 6 114 1 147 131 0.0 6 129 2 148 194 0.0 6 192 2 149 169 0.0 6 168 1 150 134 0.0 6 133 1 151 189 0.0 6 187 2 152 104 0.0 6 103 1 153 116 0.0 6 114 2 154 117 0.0 6 115 1 1 155 92 0.0 6 90 2 156 97 0.0 6 97 157 98 0.0 6 97 1 158 77 0.0 6 77 159 80 0.0 6 77 3 160 86 0.0 6 83 3 161 124 0.0 6 123 1 162 126 0.0 6 126 163 158 0.0 6 156 2 164 159 0.0 6 158 1 165 120 0.0 6 120 166 127 0.0 6 125 2 167 98 0.0 6 95 3 168 96 0.0 6 93 3 169 101 0.0 6 99 2 170 108 0.0 6 105 3 171 114 0.0 6 110 3 0 0 0 0 1 172 100 0.0 6 100 173 134 0.0 6 131 2 0 0 0 1 174 126 0.0 6 126 175 189 0.0 6 187 2 176 132 0.0 6 130 2 177 147 0.0 6 144 3 178 148 0.0 6 145 3 179 132 0.0 6 131 0 0 1 180 131 0.0 6 130 1 181 398 0.0 6 395 3 182 161 0.0 6 158 3 183 166 0.0 6 162 4 184 199 0.0 6 196 3 185 180 0.0 6 177 3 186 152 0.0 6 151 1 187 140 0.0 6 137 3 188 268 0.0 6 262 6 189 275 0.0 6 270 5 190 174 0.0 6 171 2 1 191 271 0.0 6 266 4 1 192 312 0.0 6 306 6 193 359 0.0 6 352 6 0 0 0 0 1 194 316 0.0 6 313 3 195 451 0.0 6 445 6 196 591 0.0 6 579 12 197 455 0.0 6 444 11 198 414 0.0 6 405 9 199 321 0.0 6 314 5 1 0 1 200 318 0.0 6 308 10 201 343 0.0 6 336 7 202 466 0.0 6 456 10 203 459 0.0 6 449 10 204 435 0.0 6 423 12 205 623 0.0 6 613 9 1 206 482 0.0 6 477 5 207 605 0.0 6 594 11 208 477 0.0 6 464 12 1 209 507 0.0 6 503 4 210 513 0.0 6 505 8 211 470 0.0 6 462 7 1 212 425 0.0 6 418 7 213 473 0.0 6 466 7 214 578 0.0 6 565 12 0 0 1 215 443 0.0 6 439 4 216 464 0.0 6 456 6 1 1 217 576 0.0 6 572 4 218 428 0.0 6 421 6 1 219 339 0.0 6 331 7 1 220 375 0.0 6 371 4 221 335 0.0 6 329 6 222 526 0.0 6 516 9 0 0 0 0 1 223 304 0.0 6 300 4 224 394 0.0 6 392 2 225 475 0.0 6 466 9 226 724 0.0 6 712 10 0 1 0 0 1 227 566 0.0 6 553 13 228 502 0.0 6 494 8 229 477 0.0 6 471 6 230 455 0.0 6 449 5 1 231 393 0.0 6 384 8 0 0 0 1 232 388 0.0 6 383 5 233 360 0.0 6 357 2 1 234 331 0.0 6 328 3 235 316 0.0 6 309 6 0 0 1 236 295 0.0 6 293 2 237 405 0.0 6 398 7 238 343 0.0 6 332 11 239 464 0.0 6 451 13 240 504 0.0 6 492 12 241 934 0.0 6 923 11 242 683 0.0 6 670 13 243 863 0.0 6 847 16 244 498 0.0 6 483 14 0 1 245 874 0.0 6 861 12 0 0 0 0 1 246 345 0.0 6 332 13 247 488 0.0 6 480 8 248 255 0.0 6 246 9 249 754 0.0 6 729 25 250 554 0.0 6 539 15 251 565 0.0 6 555 9 1 252 466 0.0 6 460 6 253 382 0.0 6 372 10 254 633 0.0 6 623 9 1 255 550 0.0 6 536 14 256 410 0.0 6 394 16 257 514 0.0 6 500 12 1 0 0 0 1 258 598 0.0 6 587 11 259 473 0.0 6 462 11 260 555 0.0 6 543 12 261 367 0.0 6 359 8 262 594 0.0 6 572 22 263 872 0.0 6 847 25 264 635 0.0 6 628 7 265 569 0.0 6 557 11 0 0 1 266 268 0.0 6 258 9 1 267 722 0.0 6 706 14 1 1 268 732 0.0 6 704 28 269 596 0.0 6 579 17 270 172 0.0 6 169 3 271 656 0.0 6 631 25 272 2851 0.0 6 2798 51 2 273 1189 0.0 6 1151 38 274 2615 0.0 6 2574 40 1 275 528 0.0 6 505 23 276 2672 0.0 6 2534 137 1 277 19108 0.0 6 18788 319 1 278 12156 0.0 6 11593 526 32 4 1 279 528 0.0 6 500 27 1 280 84 0.0 6 74 3 4 2 1 281 7 0.0 6 5 0 0 1 0 1 283 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 475 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 64 3 0.0 6 3 72 1 0.0 6 1 74 5 0.0 6 5 75 3 0.0 6 3 77 2 0.0 6 2 78 3 0.0 6 3 79 5 0.0 6 5 81 3 0.0 6 3 82 3 0.0 6 3 83 2 0.0 6 2 84 2 0.0 6 2 85 2 0.0 6 2 86 4 0.0 6 4 87 1 0.0 6 1 88 1 0.0 6 1 89 1 0.0 6 1 90 1 0.0 6 1 91 2 0.0 6 2 92 4 0.0 6 4 93 3 0.0 6 3 94 3 0.0 6 3 95 3 0.0 6 3 96 1 0.0 6 1 98 4 0.0 6 4 99 4 0.0 6 4 100 1 0.0 6 1 101 3 0.0 6 3 102 2 0.0 6 2 103 4 0.0 6 4 104 2 0.0 6 2 105 1 0.0 6 1 106 3 0.0 6 3 107 2 0.0 6 2 108 2 0.0 6 2 110 5 0.0 6 5 111 7 0.0 6 7 112 1 0.0 6 1 114 2 0.0 6 2 115 1 0.0 6 1 116 1 0.0 6 1 117 1 0.0 6 1 118 4 0.0 6 4 119 2 0.0 6 2 121 3 0.0 6 3 122 2 0.0 6 2 123 1 0.0 6 1 124 4 0.0 6 4 125 2 0.0 6 2 126 3 0.0 6 3 127 3 0.0 6 3 128 4 0.0 6 4 129 5 0.0 6 5 130 3 0.0 6 3 131 4 0.0 6 4 132 2 0.0 6 2 133 1 0.0 6 1 134 3 0.0 6 3 135 5 0.0 6 5 137 5 0.0 6 5 138 1 0.0 6 1 139 2 0.0 6 2 140 3 0.0 6 3 141 4 0.0 6 4 142 4 0.0 6 4 143 4 0.0 6 4 144 4 0.0 6 4 145 4 0.0 6 4 146 2 0.0 6 2 147 1 0.0 6 1 148 4 0.0 6 4 149 2 0.0 6 2 150 4 0.0 6 4 151 1 0.0 6 1 152 3 0.0 6 3 153 2 0.0 6 2 154 1 0.0 6 1 155 1 0.0 6 1 156 3 0.0 6 3 157 1 0.0 6 1 158 4 0.0 6 4 159 2 0.0 6 2 160 2 0.0 6 2 161 7 0.0 6 7 162 7 0.0 6 7 163 3 0.0 6 3 165 2 0.0 6 2 166 3 0.0 6 3 167 3 0.0 6 3 168 3 0.0 6 3 170 3 0.0 6 3 171 3 0.0 6 3 172 2 0.0 6 2 173 4 0.0 6 4 174 3 0.0 6 3 175 5 0.0 6 5 176 6 0.0 6 6 177 5 0.0 6 5 178 2 0.0 6 2 179 4 0.0 6 4 180 2 0.0 6 2 181 3 0.0 6 3 182 1 0.0 6 1 183 2 0.0 6 2 184 2 0.0 6 2 185 4 0.0 6 4 186 4 0.0 6 4 187 2 0.0 6 2 188 4 0.0 6 4 189 5 0.0 6 5 190 2 0.0 6 2 191 9 0.0 6 8 1 192 4 0.0 6 4 194 4 0.0 6 4 195 3 0.0 6 3 196 2 0.0 6 2 197 5 0.0 6 5 198 2 0.0 6 2 199 1 0.0 6 1 200 2 0.0 6 2 201 1 0.0 6 1 202 3 0.0 6 3 203 6 0.0 6 6 204 7 0.0 6 7 206 7 0.0 6 7 208 3 0.0 6 3 209 1 0.0 6 1 210 1 0.0 6 1 211 5 0.0 6 5 212 1 0.0 6 1 213 5 0.0 6 5 214 3 0.0 6 3 215 4 0.0 6 4 216 4 0.0 6 4 217 3 0.0 6 3 218 2 0.0 6 2 219 2 0.0 6 2 220 3 0.0 6 3 221 2 0.0 6 2 222 1 0.0 6 1 223 2 0.0 6 2 224 3 0.0 6 3 225 3 0.0 6 3 226 1 0.0 6 1 227 1 0.0 6 1 228 3 0.0 6 3 229 1 0.0 6 1 230 2 0.0 6 2 231 2 0.0 6 2 232 2 0.0 6 2 234 3 0.0 6 3 235 1 0.0 6 1 236 2 0.0 6 2 237 2 0.0 6 2 238 1 0.0 6 1 239 2 0.0 6 2 240 1 0.0 6 1 243 1 0.0 6 1 244 2 0.0 6 2 245 1 0.0 6 1 247 1 0.0 6 1 248 1 0.0 6 1 249 2 0.0 6 2 250 1 0.0 6 1 251 1 0.0 6 1 254 1 0.0 6 1 255 2 0.0 6 2 256 3 0.0 6 3 258 1 0.0 6 1 260 3 0.0 6 3 261 2 0.0 6 2 262 2 0.0 6 2 263 1 0.0 6 1 267 1 0.0 6 1 275 1 0.0 6 1 277 1 0.0 6 1 280 1 0.0 6 1 283 1 0.0 6 1 289 1 0.0 6 1 301 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 89298 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1402.5 0 1 1 4 1 350.6 1 0 1 5 1 87.7 1 1 6 1 21.9 1 1 7 1 5.5 2 1 9 1 0.3 2 1 10 7 0.1 3 6 1 11 14 0.0 3 11 3 12 2 0.0 3 0 2 13 11 0.0 3 10 1 14 8 0.0 4 8 15 2 0.0 4 2 16 15 0.0 4 15 17 25 0.0 5 22 2 1 18 20 0.0 5 16 4 19 5 0.0 5 4 1 20 100 0.0 6 94 5 1 21 64 0.0 6 61 3 22 41 0.0 6 38 2 1 23 23 0.0 6 22 1 24 20 0.0 6 17 3 25 19 0.0 6 18 0 0 1 26 23 0.0 6 21 2 27 15 0.0 6 14 1 28 13 0.0 6 13 29 26 0.0 6 21 4 1 30 21 0.0 6 19 2 31 17 0.0 6 15 2 32 9 0.0 6 9 33 15 0.0 6 14 1 34 20 0.0 6 19 1 35 21 0.0 6 18 3 36 9 0.0 6 7 1 1 37 14 0.0 6 13 1 38 18 0.0 6 18 39 16 0.0 6 15 1 40 12 0.0 6 9 3 41 12 0.0 6 11 1 42 7 0.0 6 6 1 43 25 0.0 6 21 4 44 28 0.0 6 28 45 12 0.0 6 10 2 46 18 0.0 6 15 3 47 23 0.0 6 20 2 1 48 25 0.0 6 23 2 49 20 0.0 6 16 2 1 0 0 1 50 17 0.0 6 17 51 18 0.0 6 18 52 34 0.0 6 31 2 0 0 1 53 31 0.0 6 28 2 0 0 1 54 27 0.0 6 26 1 55 22 0.0 6 20 1 1 56 22 0.0 6 20 2 57 32 0.0 6 29 3 58 21 0.0 6 19 1 0 0 1 59 19 0.0 6 15 2 1 0 1 60 23 0.0 6 22 1 61 20 0.0 6 19 1 62 17 0.0 6 16 1 63 23 0.0 6 20 2 1 64 27 0.0 6 26 1 65 19 0.0 6 18 1 66 31 0.0 6 28 2 1 67 163 0.0 6 156 6 1 68 48 0.0 6 46 2 69 74 0.0 6 69 3 0 0 1 0 1 70 33 0.0 6 32 1 71 43 0.0 6 39 4 72 36 0.0 6 33 3 73 17 0.0 6 14 3 74 24 0.0 6 23 1 75 32 0.0 6 29 2 0 0 0 1 76 33 0.0 6 29 4 77 32 0.0 6 32 78 39 0.0 6 34 4 0 1 79 21 0.0 6 19 2 80 34 0.0 6 31 3 81 40 0.0 6 35 4 0 1 82 49 0.0 6 46 3 83 88 0.0 6 80 7 0 0 1 84 47 0.0 6 44 3 85 38 0.0 6 35 3 86 44 0.0 6 42 2 87 66 0.0 6 63 2 1 88 42 0.0 6 40 2 89 45 0.0 6 44 0 0 0 1 90 57 0.0 6 53 4 91 52 0.0 6 47 4 1 92 34 0.0 6 32 2 93 55 0.0 6 52 3 94 87 0.0 6 83 4 95 57 0.0 6 55 2 96 48 0.0 6 46 2 97 46 0.0 6 41 4 0 0 1 98 67 0.0 6 63 3 1 99 50 0.0 6 46 3 0 0 0 0 1 100 66 0.0 6 58 6 2 101 60 0.0 6 57 3 102 97 0.0 6 91 5 0 0 1 103 117 0.0 6 112 3 1 0 1 104 138 0.0 6 128 9 0 0 1 105 120 0.0 6 113 7 106 74 0.0 6 68 6 107 90 0.0 6 84 6 108 64 0.0 6 61 3 109 81 0.0 6 78 2 0 0 0 0 1 110 88 0.0 6 83 4 1 111 93 0.0 6 87 5 0 0 0 0 1 112 103 0.0 6 97 6 113 86 0.0 6 80 6 114 109 0.0 6 103 6 115 97 0.0 6 90 5 1 0 0 0 1 116 109 0.0 6 105 3 1 117 112 0.0 6 110 2 118 124 0.0 6 113 10 1 119 116 0.0 6 111 5 120 109 0.0 6 105 4 121 117 0.0 6 113 4 122 100 0.0 6 96 3 0 0 0 0 1 123 142 0.0 6 137 5 124 157 0.0 6 152 4 0 0 1 125 169 0.0 6 161 7 0 1 126 138 0.0 6 132 5 0 0 1 127 196 0.0 6 182 12 1 0 1 128 143 0.0 6 136 6 0 0 1 129 122 0.0 6 119 2 1 130 161 0.0 6 158 3 131 242 0.0 6 236 6 132 244 0.0 6 240 3 0 0 0 0 1 133 201 0.0 6 194 4 2 0 1 134 344 0.0 6 327 14 1 1 1 135 403 0.0 6 397 5 1 136 256 0.0 6 247 9 137 282 0.0 6 271 11 138 190 0.0 6 188 1 0 0 1 139 247 0.0 6 237 10 140 394 0.0 6 383 9 0 0 2 141 407 0.0 6 391 14 1 0 1 142 525 0.0 6 505 18 1 0 1 143 530 0.0 6 518 11 1 144 616 0.0 6 590 24 0 1 0 0 1 145 450 0.0 6 438 10 0 0 1 1 146 556 0.0 6 543 11 0 0 2 147 445 0.0 6 436 8 0 1 148 547 0.0 6 534 13 149 783 0.0 6 762 17 2 0 0 2 150 1077 0.0 6 1061 15 0 0 1 151 503 0.0 6 489 11 1 1 0 0 1 152 1027 0.0 6 998 26 1 0 1 0 1 153 704 0.0 6 685 17 1 0 0 1 154 588 0.0 6 569 17 1 0 1 155 645 0.0 6 627 15 2 0 1 156 580 0.0 6 563 15 0 1 1 157 773 0.0 6 758 10 3 1 0 1 158 765 0.0 6 752 11 0 1 0 1 159 494 0.0 6 485 7 1 0 1 160 658 0.0 6 646 12 161 501 0.0 6 491 8 2 162 457 0.0 6 444 11 0 0 0 2 163 651 0.0 6 641 8 1 1 164 628 0.0 6 608 17 1 1 1 165 702 0.0 6 688 13 0 0 1 166 756 0.0 6 740 14 0 0 1 1 167 584 0.0 6 559 22 1 0 2 168 843 0.0 6 824 19 169 528 0.0 6 517 11 170 497 0.0 6 484 11 0 0 1 0 1 171 747 0.0 6 728 13 1 1 3 1 172 627 0.0 6 608 19 173 828 0.0 6 810 15 2 1 174 960 0.0 6 928 30 0 1 0 1 175 1171 0.0 6 1140 28 2 0 1 176 967 0.0 6 939 26 1 0 1 177 848 0.0 6 827 18 2 0 1 178 723 0.0 6 703 17 2 1 179 778 0.0 6 758 18 0 0 1 0 1 180 610 0.0 6 586 23 1 181 777 0.0 6 761 15 0 1 182 376 0.0 6 365 11 183 1050 0.0 6 1012 35 1 0 2 184 663 0.0 6 648 14 1 185 981 0.0 6 940 40 1 186 1750 0.0 6 1688 59 1 0 2 187 1462 0.0 6 1420 41 0 0 1 188 1377 0.0 6 1327 48 1 0 0 1 189 1076 0.0 6 1026 49 0 0 0 0 1 190 2026 0.0 6 1957 65 2 0 2 191 2003 0.0 6 1944 56 2 0 1 192 1111 0.0 6 1075 32 4 193 668 0.0 6 649 14 1 2 2 194 1300 0.0 6 1264 36 195 1706 0.0 6 1635 67 0 3 1 196 3313 0.0 6 3220 89 3 1 197 1046 0.0 6 995 43 6 1 0 1 198 1925 0.0 6 1864 58 1 1 1 199 3239 0.0 6 3150 83 1 1 4 200 1444 0.0 6 1393 47 3 1 201 1930 0.0 6 1876 54 202 1005 0.0 6 987 16 1 1 203 828 0.0 6 799 29 204 1196 0.0 6 1157 38 0 0 1 205 461 0.0 6 438 20 1 0 2 206 488 0.0 6 475 13 207 267 0.0 6 261 6 208 530 0.0 6 514 13 0 2 1 209 517 0.0 6 502 13 1 0 1 210 513 0.0 6 500 11 0 1 1 211 387 0.0 6 369 17 0 0 1 212 796 0.0 6 780 15 1 213 432 0.0 6 415 17 214 1656 0.0 6 1600 52 2 0 2 215 842 0.0 6 815 26 0 1 216 662 0.0 6 649 12 0 0 1 217 664 0.0 6 645 18 1 218 403 0.0 6 392 10 0 0 1 219 605 0.0 6 585 20 220 150 0.0 6 141 9 221 557 0.0 6 541 15 0 0 1 222 647 0.0 6 628 18 1 223 903 0.0 6 885 18 224 483 0.0 6 470 13 225 673 0.0 6 656 17 226 389 0.0 6 380 7 2 227 426 0.0 6 416 9 1 228 228 0.0 6 222 5 0 0 1 229 663 0.0 6 637 24 0 1 1 230 313 0.0 6 303 9 1 231 504 0.0 6 492 10 1 0 1 232 579 0.0 6 558 21 233 420 0.0 6 408 12 234 248 0.0 6 241 7 235 138 0.0 6 136 2 236 31 0.0 6 31 237 41 0.0 6 40 1 238 37 0.0 6 37 239 57 0.0 6 56 1 240 155 0.0 6 150 5 241 323 0.0 6 314 8 1 242 372 0.0 6 360 12 243 356 0.0 6 352 4 244 206 0.0 6 200 6 245 116 0.0 6 112 4 246 125 0.0 6 121 4 247 40 0.0 6 38 2 248 103 0.0 6 100 3 249 40 0.0 6 40 250 40 0.0 6 39 1 251 69 0.0 6 66 3 252 10 0.0 6 10 253 6 0.0 6 6 254 3 0.0 6 3 255 1 0.0 6 1 257 1 0.0 6 1 258 1 0.0 6 1 259 2 0.0 6 2 260 2 0.0 6 2 270 1 0.0 6 1 276 1 0.0 6 1 277 7 0.0 6 7 278 41 0.0 6 41 279 89 0.0 6 85 4 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 402 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 54 2 0.0 6 2 58 1 0.0 6 0 1 62 1 0.0 6 1 64 2 0.0 6 2 71 1 0.0 6 1 72 5 0.0 6 5 73 6 0.0 6 6 74 8 0.0 6 8 75 1 0.0 6 1 76 2 0.0 6 2 77 3 0.0 6 3 78 7 0.0 6 7 79 1 0.0 6 1 80 3 0.0 6 3 81 2 0.0 6 2 82 2 0.0 6 2 83 1 0.0 6 1 84 3 0.0 6 3 85 3 0.0 6 3 86 2 0.0 6 2 87 5 0.0 6 5 88 1 0.0 6 1 89 1 0.0 6 1 90 2 0.0 6 2 91 2 0.0 6 2 92 5 0.0 6 5 93 5 0.0 6 5 94 3 0.0 6 3 95 6 0.0 6 6 96 1 0.0 6 1 98 7 0.0 6 7 99 3 0.0 6 3 100 3 0.0 6 3 101 2 0.0 6 2 102 1 0.0 6 1 103 2 0.0 6 2 104 3 0.0 6 3 105 5 0.0 6 5 106 3 0.0 6 3 107 3 0.0 6 3 108 1 0.0 6 1 109 4 0.0 6 4 110 5 0.0 6 5 111 2 0.0 6 2 113 4 0.0 6 4 114 1 0.0 6 1 115 2 0.0 6 2 116 1 0.0 6 1 117 1 0.0 6 1 118 5 0.0 6 5 119 4 0.0 6 4 120 1 0.0 6 1 121 4 0.0 6 4 122 3 0.0 6 3 123 3 0.0 6 3 125 3 0.0 6 3 127 1 0.0 6 1 128 5 0.0 6 5 129 5 0.0 6 5 131 2 0.0 6 2 132 5 0.0 6 5 133 1 0.0 6 1 134 8 0.0 6 8 135 2 0.0 6 2 136 2 0.0 6 2 137 4 0.0 6 4 138 1 0.0 6 1 139 10 0.0 6 10 140 1 0.0 6 1 141 6 0.0 6 6 142 2 0.0 6 2 143 4 0.0 6 4 144 5 0.0 6 5 145 6 0.0 6 6 146 3 0.0 6 3 147 4 0.0 6 4 148 3 0.0 6 3 149 4 0.0 6 4 151 1 0.0 6 1 152 2 0.0 6 2 153 2 0.0 6 2 154 4 0.0 6 4 155 3 0.0 6 3 156 7 0.0 6 7 157 1 0.0 6 1 158 3 0.0 6 3 159 2 0.0 6 2 160 1 0.0 6 1 161 3 0.0 6 3 162 5 0.0 6 5 163 2 0.0 6 2 164 5 0.0 6 5 165 8 0.0 6 8 166 3 0.0 6 3 167 4 0.0 6 4 169 6 0.0 6 6 170 3 0.0 6 3 171 1 0.0 6 1 172 4 0.0 6 4 173 5 0.0 6 5 174 5 0.0 6 5 175 2 0.0 6 2 176 1 0.0 6 1 177 2 0.0 6 2 178 5 0.0 6 5 179 2 0.0 6 2 181 3 0.0 6 3 182 4 0.0 6 4 183 3 0.0 6 3 185 7 0.0 6 7 186 2 0.0 6 2 187 2 0.0 6 2 188 4 0.0 6 4 189 4 0.0 6 4 191 2 0.0 6 2 192 1 0.0 6 1 193 1 0.0 6 1 195 2 0.0 6 2 196 2 0.0 6 2 198 2 0.0 6 2 200 1 0.0 6 1 201 1 0.0 6 1 202 1 0.0 6 1 203 5 0.0 6 5 204 2 0.0 6 2 206 1 0.0 6 1 207 1 0.0 6 1 208 3 0.0 6 3 209 2 0.0 6 2 210 1 0.0 6 1 213 3 0.0 6 3 216 2 0.0 6 2 242 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_04_27_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_04_254_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_04_27_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_04_254_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.23 s (226654 µs/read; 0.00 M reads/minute). === Summary === Total read pairs processed: 1 Read 1 with adapter: 1 (100.0%) Read 2 with adapter: 1 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 0 (0.0%) Pairs written (passing filters): 1 (100.0%) Total basepairs processed: 414 bp Read 1: 258 bp Read 2: 156 bp Total written (filtered): 62 bp (15.0%) Read 1: 33 bp Read 2: 29 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 1 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 225 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 0 times === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 1 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 127 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 0 times This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_05_28_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_05_255_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_05_28_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_05_255_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.51 s (30 µs/read; 2.03 M reads/minute). === Summary === Total read pairs processed: 84,898 Read 1 with adapter: 84,895 (100.0%) Read 2 with adapter: 84,861 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 40 (0.0%) Pairs written (passing filters): 84,858 (100.0%) Total basepairs processed: 43,505,781 bp Read 1: 25,020,485 bp Read 2: 18,485,296 bp Total written (filtered): 5,345,805 bp (12.3%) Read 1: 2,800,223 bp Read 2: 2,545,582 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 84881 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1326.5 0 1 4 2 331.6 1 1 1 8 1 1.3 2 0 0 0 1 9 2 0.3 2 1 1 10 1 0.1 3 1 11 1 0.0 3 1 13 1 0.0 3 0 1 15 1 0.0 4 1 17 1 0.0 5 1 19 1 0.0 5 1 20 13 0.0 6 13 21 7 0.0 6 7 22 4 0.0 6 4 23 1 0.0 6 1 25 11 0.0 6 11 26 5 0.0 6 5 27 5 0.0 6 5 28 1 0.0 6 1 29 2 0.0 6 2 30 1 0.0 6 1 31 3 0.0 6 3 32 2 0.0 6 1 1 33 3 0.0 6 3 34 2 0.0 6 1 1 35 2 0.0 6 2 36 4 0.0 6 4 37 2 0.0 6 1 0 0 0 0 0 1 38 5 0.0 6 5 39 1 0.0 6 1 40 1 0.0 6 1 41 4 0.0 6 4 42 2 0.0 6 2 43 4 0.0 6 2 2 44 5 0.0 6 5 45 3 0.0 6 3 46 2 0.0 6 2 47 2 0.0 6 1 1 48 2 0.0 6 1 1 49 1 0.0 6 0 1 50 3 0.0 6 3 52 1 0.0 6 1 53 4 0.0 6 4 54 5 0.0 6 5 55 3 0.0 6 3 56 4 0.0 6 4 57 2 0.0 6 2 58 1 0.0 6 1 59 2 0.0 6 2 60 1 0.0 6 1 61 2 0.0 6 1 1 62 6 0.0 6 6 63 6 0.0 6 6 64 4 0.0 6 3 1 67 1 0.0 6 1 68 1 0.0 6 1 69 2 0.0 6 2 70 1 0.0 6 1 71 1 0.0 6 1 72 1 0.0 6 1 73 5 0.0 6 4 1 76 1 0.0 6 1 77 1 0.0 6 1 78 9 0.0 6 9 81 2 0.0 6 2 82 1 0.0 6 1 84 2 0.0 6 2 86 14 0.0 6 14 87 2 0.0 6 2 88 1 0.0 6 1 89 9 0.0 6 9 90 3 0.0 6 3 91 3 0.0 6 2 1 92 8 0.0 6 7 1 93 2 0.0 6 2 94 2 0.0 6 2 95 2 0.0 6 2 97 2 0.0 6 2 98 6 0.0 6 6 99 5 0.0 6 5 100 2 0.0 6 2 101 13 0.0 6 13 102 4 0.0 6 4 103 7 0.0 6 6 1 104 1 0.0 6 1 105 6 0.0 6 6 106 1 0.0 6 1 107 7 0.0 6 7 108 18 0.0 6 15 3 109 1 0.0 6 1 111 9 0.0 6 8 1 112 5 0.0 6 5 113 3 0.0 6 3 114 9 0.0 6 8 1 115 5 0.0 6 5 116 2 0.0 6 2 117 8 0.0 6 8 118 6 0.0 6 6 119 7 0.0 6 6 1 120 3 0.0 6 3 121 9 0.0 6 8 0 0 1 122 23 0.0 6 19 4 123 17 0.0 6 15 2 124 7 0.0 6 4 2 1 125 5 0.0 6 4 1 126 9 0.0 6 8 1 127 6 0.0 6 6 128 21 0.0 6 20 1 129 10 0.0 6 7 2 1 130 8 0.0 6 8 131 1 0.0 6 1 132 17 0.0 6 17 133 20 0.0 6 19 1 134 19 0.0 6 19 135 32 0.0 6 30 2 136 17 0.0 6 16 1 137 32 0.0 6 29 2 1 138 21 0.0 6 19 2 139 25 0.0 6 22 3 140 29 0.0 6 27 2 141 53 0.0 6 53 142 33 0.0 6 32 1 143 36 0.0 6 35 1 144 36 0.0 6 34 2 145 59 0.0 6 59 146 53 0.0 6 52 1 147 23 0.0 6 22 1 148 19 0.0 6 17 2 149 20 0.0 6 16 4 150 16 0.0 6 15 1 151 25 0.0 6 24 1 152 12 0.0 6 11 1 153 19 0.0 6 18 1 154 16 0.0 6 15 1 155 29 0.0 6 29 156 47 0.0 6 46 1 157 19 0.0 6 18 1 158 45 0.0 6 44 0 1 159 18 0.0 6 16 2 160 25 0.0 6 22 3 161 38 0.0 6 37 1 162 20 0.0 6 20 163 34 0.0 6 34 164 50 0.0 6 48 2 165 38 0.0 6 38 166 15 0.0 6 14 1 167 24 0.0 6 24 168 38 0.0 6 36 2 169 28 0.0 6 27 1 170 48 0.0 6 46 2 171 57 0.0 6 56 1 172 80 0.0 6 78 2 173 68 0.0 6 66 2 174 64 0.0 6 61 3 175 58 0.0 6 55 3 176 71 0.0 6 70 1 177 52 0.0 6 51 1 178 50 0.0 6 50 179 57 0.0 6 56 1 180 53 0.0 6 49 4 181 71 0.0 6 68 3 182 88 0.0 6 85 3 183 50 0.0 6 47 1 2 184 97 0.0 6 95 2 185 157 0.0 6 151 6 186 134 0.0 6 130 4 187 96 0.0 6 94 2 188 126 0.0 6 122 4 189 150 0.0 6 149 1 190 132 0.0 6 127 5 191 121 0.0 6 119 2 192 164 0.0 6 158 6 193 181 0.0 6 178 2 1 194 189 0.0 6 183 6 195 270 0.0 6 261 9 196 261 0.0 6 255 6 197 202 0.0 6 193 9 198 235 0.0 6 229 6 199 223 0.0 6 216 7 200 190 0.0 6 186 3 1 201 188 0.0 6 183 4 1 202 210 0.0 6 201 9 203 201 0.0 6 192 9 204 254 0.0 6 250 4 205 315 0.0 6 303 10 2 206 293 0.0 6 287 6 207 247 0.0 6 241 6 208 196 0.0 6 192 4 209 186 0.0 6 180 6 210 228 0.0 6 226 2 211 155 0.0 6 152 3 212 131 0.0 6 128 3 213 255 0.0 6 245 10 214 296 0.0 6 280 16 215 188 0.0 6 183 4 1 216 195 0.0 6 188 7 217 247 0.0 6 237 10 218 237 0.0 6 230 7 219 186 0.0 6 182 4 220 132 0.0 6 130 2 221 149 0.0 6 146 2 1 222 284 0.0 6 277 7 223 282 0.0 6 274 8 224 272 0.0 6 263 9 225 278 0.0 6 273 5 226 327 0.0 6 321 6 227 241 0.0 6 239 2 228 196 0.0 6 187 8 1 229 228 0.0 6 220 8 230 204 0.0 6 202 2 231 155 0.0 6 150 5 232 177 0.0 6 170 7 233 128 0.0 6 125 3 234 149 0.0 6 149 235 128 0.0 6 124 4 236 192 0.0 6 189 3 237 331 0.0 6 320 11 238 340 0.0 6 325 15 239 331 0.0 6 321 10 240 340 0.0 6 330 10 241 533 0.0 6 519 14 242 442 0.0 6 430 12 243 379 0.0 6 373 5 1 244 233 0.0 6 230 3 245 421 0.0 6 410 10 1 246 274 0.0 6 263 11 247 332 0.0 6 320 12 248 308 0.0 6 291 16 1 249 419 0.0 6 406 13 250 368 0.0 6 354 14 251 416 0.0 6 406 10 252 285 0.0 6 277 8 253 245 0.0 6 241 4 254 398 0.0 6 388 8 1 1 255 378 0.0 6 364 13 1 256 380 0.0 6 370 10 257 352 0.0 6 346 6 258 358 0.0 6 342 14 2 259 618 0.0 6 596 21 1 260 460 0.0 6 451 8 1 261 296 0.0 6 287 9 262 307 0.0 6 293 13 0 1 263 436 0.0 6 423 13 264 318 0.0 6 310 8 265 481 0.0 6 466 15 266 273 0.0 6 268 4 1 267 436 0.0 6 423 13 268 696 0.0 6 672 24 269 544 0.0 6 521 23 270 413 0.0 6 403 9 1 271 336 0.0 6 323 13 272 1226 0.0 6 1199 26 1 273 1183 0.0 6 1146 37 274 1502 0.0 6 1468 33 0 0 1 275 464 0.0 6 444 19 0 1 276 1398 0.0 6 1361 34 1 2 277 10467 0.0 6 10155 306 4 0 1 0 1 278 41855 0.0 6 41156 684 12 3 279 173 0.0 6 161 12 280 26 0.0 6 25 1 281 8 0.0 6 7 0 0 0 1 283 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 14 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 73 1 0.0 6 1 85 2 0.0 6 2 88 1 0.0 6 1 94 1 0.0 6 1 105 1 0.0 6 1 115 1 0.0 6 1 128 1 0.0 6 1 130 2 0.0 6 2 131 1 0.0 6 1 138 1 0.0 6 1 139 1 0.0 6 1 157 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 84846 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 2 20.7 1 1 1 8 1 1.3 2 1 10 1 0.1 3 1 11 1 0.0 3 1 12 6 0.0 3 6 13 10 0.0 3 9 1 14 5 0.0 4 5 15 1 0.0 4 1 16 15 0.0 4 13 2 17 11 0.0 5 10 1 18 8 0.0 5 7 1 19 13 0.0 5 11 2 20 45 0.0 6 42 3 21 68 0.0 6 65 3 22 45 0.0 6 44 1 23 35 0.0 6 30 5 24 27 0.0 6 25 2 25 29 0.0 6 28 1 26 9 0.0 6 8 1 27 7 0.0 6 7 28 2 0.0 6 2 29 13 0.0 6 13 30 19 0.0 6 19 31 8 0.0 6 8 32 7 0.0 6 7 33 5 0.0 6 5 34 15 0.0 6 15 35 10 0.0 6 9 1 36 11 0.0 6 9 2 37 16 0.0 6 16 38 16 0.0 6 14 2 39 12 0.0 6 11 1 40 12 0.0 6 11 0 0 1 41 6 0.0 6 6 42 5 0.0 6 4 1 43 17 0.0 6 16 1 44 24 0.0 6 21 3 45 12 0.0 6 11 1 46 23 0.0 6 21 2 47 18 0.0 6 16 2 48 17 0.0 6 16 1 49 16 0.0 6 14 2 50 30 0.0 6 27 3 51 9 0.0 6 9 52 20 0.0 6 17 1 2 53 8 0.0 6 8 54 23 0.0 6 23 55 31 0.0 6 31 56 18 0.0 6 17 1 57 20 0.0 6 18 2 58 13 0.0 6 12 1 59 15 0.0 6 14 1 60 24 0.0 6 22 1 1 61 5 0.0 6 3 2 62 12 0.0 6 11 1 63 6 0.0 6 6 64 20 0.0 6 19 1 65 15 0.0 6 13 2 66 21 0.0 6 20 1 67 35 0.0 6 28 5 2 68 51 0.0 6 45 6 69 157 0.0 6 150 5 2 70 30 0.0 6 28 2 71 78 0.0 6 75 3 72 43 0.0 6 40 3 73 36 0.0 6 32 4 74 11 0.0 6 11 75 18 0.0 6 18 76 36 0.0 6 35 1 77 26 0.0 6 23 2 0 0 0 1 78 37 0.0 6 34 3 79 27 0.0 6 23 4 80 26 0.0 6 23 3 81 21 0.0 6 20 1 82 51 0.0 6 46 5 83 42 0.0 6 37 4 0 0 1 84 61 0.0 6 59 2 85 57 0.0 6 53 4 86 44 0.0 6 42 2 87 50 0.0 6 41 8 1 88 21 0.0 6 20 1 89 36 0.0 6 31 4 0 1 90 66 0.0 6 63 3 91 56 0.0 6 49 6 1 92 26 0.0 6 24 2 93 79 0.0 6 73 5 1 94 123 0.0 6 115 8 95 29 0.0 6 29 96 51 0.0 6 49 1 1 97 56 0.0 6 53 3 98 49 0.0 6 45 4 99 55 0.0 6 50 5 100 65 0.0 6 60 4 0 0 1 101 57 0.0 6 56 1 102 97 0.0 6 94 2 0 0 1 103 143 0.0 6 136 6 1 104 90 0.0 6 89 1 105 90 0.0 6 84 6 106 62 0.0 6 58 4 107 104 0.0 6 100 3 1 108 87 0.0 6 84 2 1 109 108 0.0 6 103 3 2 110 92 0.0 6 84 8 111 105 0.0 6 100 3 1 0 1 112 112 0.0 6 101 10 1 113 88 0.0 6 83 4 1 114 114 0.0 6 105 7 1 0 1 115 78 0.0 6 72 5 0 0 1 116 66 0.0 6 61 4 1 117 53 0.0 6 50 3 118 76 0.0 6 71 4 1 119 109 0.0 6 103 6 120 134 0.0 6 122 10 2 121 87 0.0 6 83 3 1 122 76 0.0 6 74 2 123 77 0.0 6 72 5 124 116 0.0 6 110 6 125 50 0.0 6 47 1 1 0 1 126 38 0.0 6 36 2 127 85 0.0 6 82 3 128 78 0.0 6 77 1 129 68 0.0 6 64 4 130 54 0.0 6 50 4 131 133 0.0 6 125 8 132 116 0.0 6 114 2 133 104 0.0 6 103 1 134 193 0.0 6 185 8 135 295 0.0 6 287 8 136 206 0.0 6 201 3 2 137 218 0.0 6 209 8 0 0 0 1 138 108 0.0 6 103 4 1 139 223 0.0 6 215 8 140 228 0.0 6 217 11 141 197 0.0 6 191 5 1 142 365 0.0 6 356 9 143 250 0.0 6 239 10 1 144 399 0.0 6 390 8 1 145 214 0.0 6 208 6 146 203 0.0 6 197 5 1 147 100 0.0 6 97 2 1 148 202 0.0 6 194 7 1 149 284 0.0 6 276 7 1 150 504 0.0 6 486 16 2 151 249 0.0 6 235 14 152 737 0.0 6 718 19 153 340 0.0 6 325 13 1 1 154 426 0.0 6 413 12 1 155 359 0.0 6 341 14 2 1 1 156 336 0.0 6 323 11 2 157 466 0.0 6 454 11 1 158 458 0.0 6 445 11 1 0 1 159 243 0.0 6 232 9 2 160 451 0.0 6 436 14 1 161 286 0.0 6 274 12 162 254 0.0 6 240 14 163 542 0.0 6 530 11 1 164 369 0.0 6 358 10 0 0 1 165 564 0.0 6 545 17 2 166 538 0.0 6 522 15 1 167 303 0.0 6 295 6 2 168 563 0.0 6 551 11 0 1 169 218 0.0 6 215 3 170 363 0.0 6 352 10 1 171 595 0.0 6 580 14 0 0 1 172 298 0.0 6 283 14 1 173 559 0.0 6 542 17 174 741 0.0 6 707 31 0 1 2 175 1359 0.0 6 1326 29 1 0 1 1 1 176 786 0.0 6 761 24 1 177 841 0.0 6 818 21 2 178 653 0.0 6 626 24 3 179 738 0.0 6 709 26 3 180 760 0.0 6 737 21 0 0 2 181 834 0.0 6 817 15 1 0 1 182 313 0.0 6 299 13 1 183 940 0.0 6 912 23 4 0 1 184 141 0.0 6 131 7 3 185 401 0.0 6 381 18 0 2 186 1983 0.0 6 1933 47 1 0 2 187 1601 0.0 6 1551 45 1 2 2 188 1606 0.0 6 1555 50 1 189 1248 0.0 6 1218 26 1 0 3 190 2447 0.0 6 2388 56 3 191 1838 0.0 6 1786 49 2 0 1 192 1277 0.0 6 1253 22 1 1 193 758 0.0 6 723 29 4 1 0 0 1 194 1498 0.0 6 1448 46 3 0 0 1 195 1951 0.0 6 1879 72 196 3724 0.0 6 3639 83 0 0 2 197 653 0.0 6 628 20 1 1 2 0 1 198 2894 0.0 6 2793 98 2 0 1 199 4469 0.0 6 4347 116 4 0 2 200 1465 0.0 6 1417 46 2 201 1863 0.0 6 1814 46 3 202 1189 0.0 6 1159 29 1 203 1188 0.0 6 1151 34 3 204 1513 0.0 6 1485 26 1 0 1 205 454 0.0 6 443 11 206 439 0.0 6 428 10 1 207 213 0.0 6 203 9 1 208 600 0.0 6 584 15 0 1 209 494 0.0 6 473 18 3 210 453 0.0 6 446 6 0 0 1 211 574 0.0 6 552 20 1 0 0 1 212 538 0.0 6 523 15 213 178 0.0 6 171 7 214 488 0.0 6 477 10 0 0 1 215 969 0.0 6 936 32 1 216 982 0.0 6 956 24 1 0 0 0 1 217 1198 0.0 6 1172 24 1 0 1 218 828 0.0 6 805 23 219 1037 0.0 6 1015 21 0 0 1 220 440 0.0 6 419 20 1 221 923 0.0 6 903 20 222 164 0.0 6 149 13 2 223 877 0.0 6 864 11 2 224 749 0.0 6 730 17 2 225 1215 0.0 6 1192 22 1 226 1001 0.0 6 972 27 0 0 2 227 660 0.0 6 645 15 228 307 0.0 6 291 16 229 803 0.0 6 783 19 0 0 1 230 441 0.0 6 422 19 231 543 0.0 6 530 13 232 580 0.0 6 566 14 233 908 0.0 6 889 19 234 407 0.0 6 401 5 0 0 1 235 219 0.0 6 212 7 236 176 0.0 6 173 3 237 92 0.0 6 90 2 238 44 0.0 6 40 3 1 239 44 0.0 6 42 2 240 252 0.0 6 246 6 241 167 0.0 6 159 8 242 294 0.0 6 288 6 243 339 0.0 6 328 11 244 168 0.0 6 164 3 0 0 1 245 108 0.0 6 103 5 246 95 0.0 6 91 4 247 55 0.0 6 54 1 248 70 0.0 6 67 3 249 76 0.0 6 74 2 250 59 0.0 6 55 4 251 38 0.0 6 37 1 252 22 0.0 6 22 253 56 0.0 6 55 1 254 10 0.0 6 10 255 9 0.0 6 8 1 256 7 0.0 6 7 257 5 0.0 6 5 259 3 0.0 6 2 1 260 1 0.0 6 1 268 1 0.0 6 1 279 5 0.0 6 5 280 20 0.0 6 20 281 61 0.0 6 57 2 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 15 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 1 0.0 6 1 71 1 0.0 6 1 75 1 0.0 6 1 84 1 0.0 6 1 87 1 0.0 6 1 92 1 0.0 6 1 93 3 0.0 6 3 102 1 0.0 6 1 113 1 0.0 6 1 127 1 0.0 6 1 136 1 0.0 6 1 149 1 0.0 6 1 156 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_06_29_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_06_256_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_06_29_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_06_256_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.90 s (25 µs/read; 2.37 M reads/minute). === Summary === Total read pairs processed: 75,150 Read 1 with adapter: 75,143 (100.0%) Read 2 with adapter: 75,122 (100.0%) == Read fate breakdown == Pairs that were too short: 24 (0.0%) Pairs discarded as untrimmed: 35 (0.0%) Pairs written (passing filters): 75,091 (99.9%) Total basepairs processed: 31,795,142 bp Read 1: 17,485,082 bp Read 2: 14,310,060 bp Total written (filtered): 4,834,249 bp (15.2%) Read 1: 2,573,623 bp Read 2: 2,260,626 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 73302 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1174.2 0 0 1 4 4 293.6 1 2 2 5 1 73.4 1 1 6 2 18.3 1 2 7 2 4.6 2 1 0 1 10 1 0.1 3 1 13 1 0.0 3 1 15 2 0.0 4 1 1 17 7 0.0 5 6 0 1 18 3 0.0 5 1 1 1 19 5 0.0 5 5 20 82 0.0 6 79 3 21 46 0.0 6 44 2 22 45 0.0 6 44 1 23 36 0.0 6 36 24 17 0.0 6 15 2 25 26 0.0 6 26 26 20 0.0 6 20 27 14 0.0 6 14 28 15 0.0 6 14 1 29 12 0.0 6 12 30 5 0.0 6 5 31 5 0.0 6 5 32 5 0.0 6 5 33 4 0.0 6 4 34 11 0.0 6 10 0 1 35 7 0.0 6 5 1 1 36 8 0.0 6 8 37 8 0.0 6 8 38 5 0.0 6 4 1 39 8 0.0 6 8 40 12 0.0 6 12 41 4 0.0 6 3 1 42 6 0.0 6 6 43 5 0.0 6 5 44 4 0.0 6 4 45 4 0.0 6 3 0 1 46 5 0.0 6 4 1 47 3 0.0 6 3 48 9 0.0 6 8 1 49 8 0.0 6 8 50 7 0.0 6 7 51 2 0.0 6 2 52 5 0.0 6 5 53 2 0.0 6 2 54 5 0.0 6 5 55 9 0.0 6 9 56 7 0.0 6 7 57 3 0.0 6 3 58 4 0.0 6 4 59 3 0.0 6 3 60 6 0.0 6 5 1 61 5 0.0 6 5 62 4 0.0 6 4 63 10 0.0 6 9 1 64 14 0.0 6 11 3 65 2 0.0 6 2 66 12 0.0 6 12 67 1 0.0 6 1 68 6 0.0 6 6 69 8 0.0 6 8 70 7 0.0 6 7 71 8 0.0 6 8 72 8 0.0 6 8 73 9 0.0 6 8 1 74 13 0.0 6 11 2 75 9 0.0 6 8 1 76 8 0.0 6 8 77 11 0.0 6 11 78 8 0.0 6 7 1 79 11 0.0 6 11 80 14 0.0 6 14 81 17 0.0 6 17 82 13 0.0 6 12 1 83 12 0.0 6 11 1 84 13 0.0 6 12 1 85 7 0.0 6 7 86 10 0.0 6 10 87 8 0.0 6 7 1 88 11 0.0 6 11 89 9 0.0 6 8 1 90 5 0.0 6 5 91 6 0.0 6 5 1 92 16 0.0 6 14 2 93 10 0.0 6 10 94 8 0.0 6 8 95 10 0.0 6 10 96 13 0.0 6 12 1 97 7 0.0 6 7 98 11 0.0 6 10 0 0 1 99 13 0.0 6 12 1 100 15 0.0 6 15 101 21 0.0 6 21 102 21 0.0 6 21 103 32 0.0 6 31 1 104 26 0.0 6 26 105 18 0.0 6 15 2 0 0 0 1 106 17 0.0 6 17 107 25 0.0 6 25 108 14 0.0 6 14 109 30 0.0 6 30 110 21 0.0 6 21 111 34 0.0 6 34 112 36 0.0 6 35 1 113 36 0.0 6 36 114 35 0.0 6 33 1 0 0 0 1 115 27 0.0 6 27 116 37 0.0 6 37 117 31 0.0 6 30 0 0 0 0 1 118 34 0.0 6 31 0 0 0 1 1 1 119 38 0.0 6 37 1 120 44 0.0 6 43 1 121 14 0.0 6 14 122 25 0.0 6 24 1 123 37 0.0 6 36 1 124 43 0.0 6 43 125 44 0.0 6 44 126 42 0.0 6 42 127 41 0.0 6 41 128 50 0.0 6 50 129 44 0.0 6 43 1 130 46 0.0 6 46 131 47 0.0 6 47 132 42 0.0 6 42 133 55 0.0 6 54 1 134 64 0.0 6 63 1 135 57 0.0 6 56 1 136 60 0.0 6 59 1 137 78 0.0 6 77 0 0 1 138 74 0.0 6 72 1 0 0 0 1 139 108 0.0 6 107 1 140 261 0.0 6 258 2 1 141 455 0.0 6 455 142 551 0.0 6 550 1 143 814 0.0 6 813 1 144 1020 0.0 6 1018 2 145 1103 0.0 6 1102 1 146 1189 0.0 6 1187 2 147 1252 0.0 6 1249 2 0 0 0 0 1 148 1074 0.0 6 1071 2 0 0 0 0 1 149 923 0.0 6 920 3 150 1439 0.0 6 1437 2 151 808 0.0 6 804 3 0 0 0 0 1 152 779 0.0 6 778 1 153 658 0.0 6 657 1 154 577 0.0 6 576 1 155 548 0.0 6 546 2 156 692 0.0 6 692 157 612 0.0 6 612 158 654 0.0 6 652 2 159 693 0.0 6 690 3 160 755 0.0 6 754 1 161 692 0.0 6 691 1 162 744 0.0 6 742 2 163 556 0.0 6 554 2 164 472 0.0 6 468 4 165 371 0.0 6 371 166 434 0.0 6 433 1 167 479 0.0 6 478 1 168 428 0.0 6 426 2 169 323 0.0 6 323 170 397 0.0 6 396 1 171 638 0.0 6 635 3 172 550 0.0 6 544 6 173 389 0.0 6 388 0 1 174 417 0.0 6 416 1 175 506 0.0 6 501 5 176 397 0.0 6 394 3 177 371 0.0 6 370 1 178 465 0.0 6 464 1 179 292 0.0 6 291 1 180 377 0.0 6 375 1 0 0 0 0 1 181 447 0.0 6 445 2 182 382 0.0 6 380 2 183 362 0.0 6 359 2 0 1 184 330 0.0 6 328 2 185 309 0.0 6 306 2 0 0 1 186 326 0.0 6 323 3 187 286 0.0 6 285 1 188 386 0.0 6 381 4 1 189 312 0.0 6 310 1 0 1 190 372 0.0 6 372 191 398 0.0 6 393 4 0 1 192 445 0.0 6 444 1 193 514 0.0 6 512 2 194 494 0.0 6 490 3 1 195 588 0.0 6 585 3 196 584 0.0 6 578 4 0 0 0 1 1 197 544 0.0 6 538 6 198 439 0.0 6 433 6 199 411 0.0 6 407 4 200 454 0.0 6 452 1 1 201 394 0.0 6 391 3 202 573 0.0 6 569 4 203 426 0.0 6 421 3 1 0 0 1 204 491 0.0 6 487 4 205 570 0.0 6 565 4 0 0 0 1 206 501 0.0 6 500 1 207 628 0.0 6 624 3 1 208 496 0.0 6 492 4 209 449 0.0 6 445 4 210 522 0.0 6 517 4 0 0 1 211 542 0.0 6 538 4 212 529 0.0 6 526 3 213 671 0.0 6 668 3 214 700 0.0 6 695 4 0 0 0 1 215 564 0.0 6 560 3 0 0 1 216 508 0.0 6 505 3 217 579 0.0 6 577 2 218 530 0.0 6 524 6 219 424 0.0 6 420 3 1 220 500 0.0 6 499 0 0 0 0 0 1 221 460 0.0 6 454 6 222 503 0.0 6 498 5 223 480 0.0 6 472 8 224 562 0.0 6 555 5 0 0 1 1 225 526 0.0 6 522 4 226 556 0.0 6 545 11 227 437 0.0 6 430 6 1 228 458 0.0 6 454 4 229 505 0.0 6 500 5 230 478 0.0 6 474 3 1 231 447 0.0 6 445 2 232 431 0.0 6 430 1 233 421 0.0 6 417 4 234 407 0.0 6 402 4 0 0 1 235 426 0.0 6 424 2 236 408 0.0 6 407 1 237 455 0.0 6 451 4 238 418 0.0 6 417 1 239 440 0.0 6 433 7 240 457 0.0 6 447 10 241 475 0.0 6 469 6 242 363 0.0 6 359 4 243 323 0.0 6 318 5 244 317 0.0 6 312 5 245 314 0.0 6 310 4 246 247 0.0 6 241 6 247 311 0.0 6 307 3 0 0 0 1 248 277 0.0 6 271 5 0 0 0 1 249 312 0.0 6 306 6 250 361 0.0 6 356 4 0 0 1 251 268 0.0 6 264 4 252 273 0.0 6 268 5 253 244 0.0 6 242 1 0 0 1 254 295 0.0 6 293 2 255 299 0.0 6 295 4 256 304 0.0 6 301 2 1 257 291 0.0 6 286 5 258 299 0.0 6 290 9 259 361 0.0 6 355 5 1 260 243 0.0 6 237 6 261 241 0.0 6 239 2 262 183 0.0 6 182 1 263 273 0.0 6 265 6 1 1 264 213 0.0 6 208 5 265 260 0.0 6 255 5 266 184 0.0 6 180 4 267 247 0.0 6 243 4 268 404 0.0 6 397 7 269 202 0.0 6 196 5 0 0 1 270 153 0.0 6 152 0 1 271 170 0.0 6 164 5 1 272 368 0.0 6 363 5 273 445 0.0 6 438 7 274 281 0.0 6 278 3 275 332 0.0 6 327 4 1 276 581 0.0 6 562 17 1 0 0 1 277 2705 0.0 6 2647 58 278 2979 0.0 6 2916 62 0 0 1 279 35 0.0 6 33 2 280 8 0.0 6 8 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1841 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 8 0.0 6 2 6 64 132 0.0 6 127 5 65 1 0.0 6 1 68 1 0.0 6 1 72 4 0.0 6 4 73 15 0.0 6 15 74 19 0.0 6 19 75 20 0.0 6 20 76 31 0.0 6 31 77 32 0.0 6 32 78 49 0.0 6 49 79 37 0.0 6 37 80 31 0.0 6 31 81 29 0.0 6 29 82 35 0.0 6 35 83 38 0.0 6 38 84 25 0.0 6 25 85 25 0.0 6 25 86 20 0.0 6 20 87 17 0.0 6 17 88 17 0.0 6 17 89 24 0.0 6 24 90 12 0.0 6 12 91 29 0.0 6 29 92 22 0.0 6 22 93 25 0.0 6 24 0 0 0 0 0 1 94 18 0.0 6 18 95 32 0.0 6 32 96 21 0.0 6 21 97 14 0.0 6 14 98 10 0.0 6 10 99 12 0.0 6 12 100 9 0.0 6 9 101 14 0.0 6 13 1 102 9 0.0 6 9 103 19 0.0 6 19 104 22 0.0 6 22 105 17 0.0 6 17 106 7 0.0 6 7 107 14 0.0 6 12 2 108 10 0.0 6 10 109 9 0.0 6 9 110 14 0.0 6 14 111 16 0.0 6 16 112 4 0.0 6 4 113 13 0.0 6 13 114 12 0.0 6 12 115 11 0.0 6 11 116 11 0.0 6 11 117 11 0.0 6 11 118 2 0.0 6 2 119 4 0.0 6 4 120 8 0.0 6 8 121 13 0.0 6 13 122 9 0.0 6 9 123 6 0.0 6 6 124 8 0.0 6 8 125 9 0.0 6 9 126 8 0.0 6 8 127 10 0.0 6 10 128 8 0.0 6 8 129 9 0.0 6 9 130 1 0.0 6 1 131 3 0.0 6 3 132 7 0.0 6 7 134 5 0.0 6 5 135 11 0.0 6 11 136 4 0.0 6 4 137 5 0.0 6 5 138 3 0.0 6 3 139 6 0.0 6 6 140 6 0.0 6 6 141 2 0.0 6 2 142 6 0.0 6 6 143 3 0.0 6 3 144 4 0.0 6 4 145 11 0.0 6 11 146 8 0.0 6 8 147 7 0.0 6 7 148 4 0.0 6 4 149 4 0.0 6 4 150 4 0.0 6 4 151 3 0.0 6 3 152 4 0.0 6 4 153 8 0.0 6 8 154 3 0.0 6 3 155 3 0.0 6 3 156 1 0.0 6 1 157 2 0.0 6 2 158 5 0.0 6 5 159 5 0.0 6 5 160 1 0.0 6 1 161 6 0.0 6 6 162 7 0.0 6 7 163 5 0.0 6 5 164 4 0.0 6 4 165 8 0.0 6 8 166 12 0.0 6 12 167 14 0.0 6 14 168 6 0.0 6 6 169 15 0.0 6 15 170 6 0.0 6 6 171 12 0.0 6 12 172 7 0.0 6 6 1 173 14 0.0 6 14 174 5 0.0 6 5 175 7 0.0 6 7 176 7 0.0 6 7 177 6 0.0 6 6 178 6 0.0 6 6 179 6 0.0 6 6 180 7 0.0 6 7 181 7 0.0 6 7 182 3 0.0 6 3 183 12 0.0 6 12 184 10 0.0 6 10 185 3 0.0 6 3 186 7 0.0 6 7 187 8 0.0 6 8 188 1 0.0 6 1 189 2 0.0 6 2 190 6 0.0 6 6 191 14 0.0 6 14 192 10 0.0 6 10 193 5 0.0 6 5 194 4 0.0 6 4 195 8 0.0 6 8 196 3 0.0 6 3 197 4 0.0 6 4 198 6 0.0 6 6 199 7 0.0 6 7 200 7 0.0 6 7 201 8 0.0 6 8 202 5 0.0 6 5 203 13 0.0 6 13 204 11 0.0 6 11 205 5 0.0 6 5 206 4 0.0 6 4 207 10 0.0 6 10 208 5 0.0 6 5 209 8 0.0 6 8 210 3 0.0 6 3 211 4 0.0 6 4 212 3 0.0 6 3 213 4 0.0 6 4 214 2 0.0 6 2 215 8 0.0 6 8 216 5 0.0 6 5 217 10 0.0 6 10 218 7 0.0 6 7 219 12 0.0 6 12 220 5 0.0 6 5 221 4 0.0 6 4 222 7 0.0 6 7 223 3 0.0 6 3 224 4 0.0 6 4 225 6 0.0 6 6 226 11 0.0 6 11 227 3 0.0 6 3 228 4 0.0 6 4 229 8 0.0 6 8 230 3 0.0 6 3 231 4 0.0 6 4 232 4 0.0 6 4 233 3 0.0 6 3 234 4 0.0 6 4 235 1 0.0 6 1 236 1 0.0 6 1 238 2 0.0 6 2 239 4 0.0 6 4 240 5 0.0 6 5 241 5 0.0 6 5 242 1 0.0 6 1 243 1 0.0 6 1 244 2 0.0 6 2 245 2 0.0 6 2 246 4 0.0 6 4 247 2 0.0 6 2 248 1 0.0 6 1 249 2 0.0 6 2 250 2 0.0 6 2 251 2 0.0 6 2 252 5 0.0 6 5 253 3 0.0 6 3 254 1 0.0 6 1 257 3 0.0 6 3 259 1 0.0 6 1 260 2 0.0 6 2 261 3 0.0 6 3 262 2 0.0 6 2 263 6 0.0 6 6 264 5 0.0 6 5 265 5 0.0 6 5 269 1 0.0 6 1 271 2 0.0 6 2 272 1 0.0 6 1 273 2 0.0 6 2 274 2 0.0 6 2 275 1 0.0 6 1 278 1 0.0 6 1 282 1 0.0 6 1 283 1 0.0 6 1 284 1 0.0 6 1 285 3 0.0 6 3 286 1 0.0 6 1 292 1 0.0 6 1 300 1 0.0 6 1 305 1 0.0 6 1 306 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 73396 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1174.2 0 1 7 1 4.6 2 1 12 2 0.0 3 1 1 13 1 0.0 3 1 14 10 0.0 4 10 15 4 0.0 4 4 16 2 0.0 4 1 1 17 16 0.0 5 14 2 18 5 0.0 5 4 1 19 35 0.0 5 31 3 1 20 79 0.0 6 77 2 21 57 0.0 6 55 2 22 39 0.0 6 36 3 23 31 0.0 6 30 1 24 30 0.0 6 30 25 20 0.0 6 17 3 26 15 0.0 6 15 27 7 0.0 6 7 28 17 0.0 6 17 29 12 0.0 6 11 0 1 30 8 0.0 6 8 31 12 0.0 6 11 1 32 13 0.0 6 11 1 0 0 0 1 33 9 0.0 6 9 34 26 0.0 6 23 2 0 1 35 13 0.0 6 11 0 1 1 36 10 0.0 6 9 1 37 7 0.0 6 7 38 19 0.0 6 16 3 39 18 0.0 6 17 1 40 14 0.0 6 14 41 13 0.0 6 9 4 42 12 0.0 6 12 43 18 0.0 6 18 44 10 0.0 6 10 45 15 0.0 6 14 1 46 17 0.0 6 17 47 21 0.0 6 19 2 48 21 0.0 6 18 2 0 0 1 49 17 0.0 6 15 1 1 50 14 0.0 6 12 2 51 21 0.0 6 19 1 0 1 52 22 0.0 6 19 2 0 0 1 53 15 0.0 6 14 1 54 20 0.0 6 17 2 1 55 21 0.0 6 19 2 56 31 0.0 6 27 2 1 1 57 29 0.0 6 27 2 58 25 0.0 6 24 0 1 59 8 0.0 6 6 2 60 19 0.0 6 18 0 1 61 23 0.0 6 20 3 62 26 0.0 6 25 0 0 0 1 63 27 0.0 6 26 1 64 24 0.0 6 24 65 24 0.0 6 23 1 66 35 0.0 6 33 2 67 27 0.0 6 25 2 68 25 0.0 6 24 0 0 0 0 1 69 50 0.0 6 43 6 0 0 1 70 142 0.0 6 137 3 0 0 1 0 1 71 55 0.0 6 53 0 0 0 0 0 2 72 87 0.0 6 82 4 0 0 1 73 41 0.0 6 40 1 74 47 0.0 6 44 2 1 75 40 0.0 6 37 1 0 2 76 20 0.0 6 20 77 43 0.0 6 40 2 1 78 41 0.0 6 38 2 1 79 35 0.0 6 35 80 33 0.0 6 32 1 81 40 0.0 6 39 0 1 82 39 0.0 6 37 2 83 63 0.0 6 58 4 0 0 0 0 1 84 54 0.0 6 53 1 85 49 0.0 6 47 1 0 0 1 86 68 0.0 6 64 4 87 51 0.0 6 48 3 88 29 0.0 6 28 1 89 45 0.0 6 43 2 90 60 0.0 6 58 1 1 91 47 0.0 6 42 4 0 0 0 1 92 59 0.0 6 57 2 93 67 0.0 6 64 2 1 94 85 0.0 6 80 4 1 95 55 0.0 6 53 2 96 75 0.0 6 72 1 1 1 97 70 0.0 6 70 98 70 0.0 6 67 0 0 1 0 1 1 99 90 0.0 6 83 6 1 100 78 0.0 6 77 1 101 70 0.0 6 66 3 0 0 0 1 102 81 0.0 6 77 4 103 84 0.0 6 81 3 104 84 0.0 6 80 2 0 0 0 1 1 105 91 0.0 6 87 3 0 0 1 106 99 0.0 6 96 1 0 1 1 107 155 0.0 6 154 1 108 82 0.0 6 77 5 109 98 0.0 6 95 3 110 85 0.0 6 83 1 1 111 85 0.0 6 84 1 112 109 0.0 6 109 113 115 0.0 6 113 2 114 135 0.0 6 129 5 1 115 133 0.0 6 128 5 116 110 0.0 6 107 3 117 129 0.0 6 128 1 118 155 0.0 6 152 2 0 1 119 122 0.0 6 117 4 1 120 185 0.0 6 181 3 0 0 0 1 121 185 0.0 6 178 5 1 0 1 122 175 0.0 6 172 2 1 123 178 0.0 6 173 4 0 0 1 124 169 0.0 6 164 5 125 183 0.0 6 178 4 1 126 218 0.0 6 213 5 127 256 0.0 6 254 2 128 272 0.0 6 271 1 129 268 0.0 6 262 5 0 0 0 1 130 279 0.0 6 279 131 359 0.0 6 349 9 1 132 387 0.0 6 371 15 0 0 0 1 133 366 0.0 6 355 9 0 1 1 134 360 0.0 6 356 2 1 0 1 135 372 0.0 6 369 3 136 440 0.0 6 432 7 0 0 0 1 137 462 0.0 6 455 5 0 0 2 138 435 0.0 6 424 8 1 0 0 1 1 139 455 0.0 6 448 7 140 512 0.0 6 505 6 0 0 0 1 141 599 0.0 6 592 7 142 846 0.0 6 837 8 1 143 915 0.0 6 909 3 0 1 1 0 1 144 1216 0.0 6 1203 11 0 1 0 0 1 145 1417 0.0 6 1403 12 1 0 0 0 1 146 1269 0.0 6 1261 7 1 147 1489 0.0 6 1481 8 148 1455 0.0 6 1443 9 1 1 1 149 1417 0.0 6 1404 11 0 0 0 0 2 150 1377 0.0 6 1363 12 0 0 1 1 151 1853 0.0 6 1839 12 1 0 0 0 1 152 1471 0.0 6 1457 10 1 1 0 0 2 153 1781 0.0 6 1756 18 2 1 2 1 1 154 1278 0.0 6 1259 16 0 1 0 2 155 1377 0.0 6 1347 22 1 2 1 2 2 156 1352 0.0 6 1338 11 1 0 0 2 157 1210 0.0 6 1191 13 2 2 0 1 1 158 1337 0.0 6 1317 17 1 0 1 0 1 159 1135 0.0 6 1118 17 160 1290 0.0 6 1276 11 0 1 1 0 1 161 1245 0.0 6 1218 24 1 0 1 0 1 162 1012 0.0 6 1002 8 1 0 0 1 163 1057 0.0 6 1045 10 1 0 0 0 1 164 966 0.0 6 949 15 1 1 165 837 0.0 6 829 8 166 885 0.0 6 873 10 0 0 0 1 1 167 745 0.0 6 731 12 0 0 1 1 168 828 0.0 6 816 9 2 0 0 1 169 892 0.0 6 884 7 0 0 0 0 1 170 712 0.0 6 701 10 0 1 171 938 0.0 6 926 11 0 0 1 172 817 0.0 6 809 7 1 173 816 0.0 6 811 3 1 1 174 873 0.0 6 861 12 175 961 0.0 6 951 9 0 1 176 870 0.0 6 864 4 1 1 177 759 0.0 6 753 5 0 0 0 1 178 762 0.0 6 753 8 1 179 763 0.0 6 758 3 0 0 1 0 1 180 623 0.0 6 617 4 0 0 1 0 1 181 788 0.0 6 775 13 182 503 0.0 6 498 5 183 535 0.0 6 525 7 0 1 1 1 184 386 0.0 6 378 8 185 417 0.0 6 409 7 1 186 673 0.0 6 664 7 0 0 2 187 853 0.0 6 835 14 2 1 0 1 188 887 0.0 6 869 17 0 0 1 189 593 0.0 6 585 7 0 0 1 190 694 0.0 6 682 10 2 191 662 0.0 6 651 10 0 0 0 0 1 192 555 0.0 6 546 8 0 0 0 1 193 474 0.0 6 466 7 0 1 194 509 0.0 6 497 11 0 0 1 195 499 0.0 6 487 11 0 1 196 647 0.0 6 632 14 1 197 412 0.0 6 406 5 0 0 1 198 828 0.0 6 813 15 199 691 0.0 6 676 14 0 0 0 1 200 542 0.0 6 531 10 0 1 201 449 0.0 6 442 7 202 363 0.0 6 355 8 203 330 0.0 6 326 4 204 235 0.0 6 230 4 0 0 1 205 231 0.0 6 223 8 206 179 0.0 6 177 2 207 204 0.0 6 199 4 0 1 208 255 0.0 6 247 7 0 1 209 194 0.0 6 194 210 221 0.0 6 213 8 211 254 0.0 6 249 4 1 212 180 0.0 6 176 4 213 94 0.0 6 92 2 214 144 0.0 6 142 2 215 149 0.0 6 146 2 0 1 216 221 0.0 6 216 5 217 231 0.0 6 225 5 0 0 1 218 144 0.0 6 142 2 219 161 0.0 6 157 3 0 0 1 220 154 0.0 6 146 8 221 127 0.0 6 124 2 1 222 129 0.0 6 125 4 223 121 0.0 6 120 1 224 111 0.0 6 110 1 225 117 0.0 6 116 1 226 111 0.0 6 106 5 227 101 0.0 6 99 2 228 94 0.0 6 94 229 76 0.0 6 75 1 230 95 0.0 6 92 3 231 71 0.0 6 69 2 232 121 0.0 6 119 2 233 56 0.0 6 53 2 0 0 1 234 40 0.0 6 39 1 235 49 0.0 6 47 2 236 24 0.0 6 22 2 237 31 0.0 6 31 238 21 0.0 6 20 1 239 18 0.0 6 17 1 240 24 0.0 6 20 4 241 44 0.0 6 41 3 242 28 0.0 6 28 243 18 0.0 6 18 244 42 0.0 6 41 1 245 41 0.0 6 41 246 17 0.0 6 17 247 11 0.0 6 11 248 16 0.0 6 16 249 12 0.0 6 12 250 9 0.0 6 9 251 19 0.0 6 18 1 252 12 0.0 6 10 2 253 5 0.0 6 5 254 23 0.0 6 23 255 5 0.0 6 5 256 3 0.0 6 3 257 1 0.0 6 1 258 2 0.0 6 2 279 2 0.0 6 2 280 2 0.0 6 2 281 10 0.0 6 10 282 72 0.0 6 71 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1726 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 3 0.0 6 0 3 48 1 0.0 6 0 1 49 15 0.0 6 15 56 1 0.0 6 1 62 1 0.0 6 1 65 4 0.0 6 4 68 17 0.0 6 17 69 3 0.0 6 3 70 32 0.0 6 32 71 14 0.0 6 14 72 44 0.0 6 44 73 34 0.0 6 34 74 46 0.0 6 46 75 65 0.0 6 65 76 44 0.0 6 44 77 62 0.0 6 62 78 51 0.0 6 50 0 0 0 1 79 60 0.0 6 59 0 0 0 1 80 34 0.0 6 33 1 81 18 0.0 6 18 82 24 0.0 6 24 83 20 0.0 6 20 84 25 0.0 6 25 85 32 0.0 6 32 86 17 0.0 6 17 87 26 0.0 6 26 88 35 0.0 6 34 1 89 31 0.0 6 31 90 22 0.0 6 22 91 28 0.0 6 27 1 92 27 0.0 6 27 93 15 0.0 6 15 94 10 0.0 6 10 95 9 0.0 6 9 96 22 0.0 6 22 97 9 0.0 6 9 98 10 0.0 6 10 99 9 0.0 6 9 100 27 0.0 6 27 101 17 0.0 6 17 102 17 0.0 6 17 103 11 0.0 6 11 104 11 0.0 6 11 105 10 0.0 6 10 106 14 0.0 6 14 107 13 0.0 6 13 108 6 0.0 6 6 109 10 0.0 6 10 110 15 0.0 6 15 111 16 0.0 6 16 112 13 0.0 6 13 113 10 0.0 6 10 114 4 0.0 6 4 115 4 0.0 6 4 116 5 0.0 6 5 117 12 0.0 6 12 118 8 0.0 6 8 119 11 0.0 6 11 120 11 0.0 6 11 121 15 0.0 6 15 122 5 0.0 6 4 1 123 6 0.0 6 6 124 4 0.0 6 4 125 20 0.0 6 20 126 8 0.0 6 8 127 5 0.0 6 5 128 5 0.0 6 5 129 6 0.0 6 6 130 4 0.0 6 4 131 17 0.0 6 17 132 3 0.0 6 3 133 11 0.0 6 11 134 3 0.0 6 3 135 5 0.0 6 5 136 5 0.0 6 5 137 8 0.0 6 8 138 6 0.0 6 6 139 5 0.0 6 5 140 6 0.0 6 6 141 10 0.0 6 10 142 9 0.0 6 9 143 3 0.0 6 3 144 5 0.0 6 5 145 3 0.0 6 3 146 10 0.0 6 9 0 1 147 3 0.0 6 3 148 6 0.0 6 6 149 6 0.0 6 6 150 4 0.0 6 4 151 4 0.0 6 4 152 4 0.0 6 4 153 6 0.0 6 6 154 6 0.0 6 6 155 9 0.0 6 9 156 6 0.0 6 6 157 3 0.0 6 3 158 6 0.0 6 6 159 9 0.0 6 9 160 5 0.0 6 5 161 6 0.0 6 6 162 9 0.0 6 9 163 15 0.0 6 15 164 4 0.0 6 4 165 6 0.0 6 5 0 0 0 0 1 166 3 0.0 6 3 167 6 0.0 6 6 168 14 0.0 6 14 169 11 0.0 6 11 170 21 0.0 6 20 1 171 5 0.0 6 5 172 8 0.0 6 8 173 5 0.0 6 5 174 5 0.0 6 5 175 1 0.0 6 1 176 7 0.0 6 7 177 9 0.0 6 8 0 0 1 178 7 0.0 6 7 179 17 0.0 6 17 180 10 0.0 6 10 181 6 0.0 6 6 182 8 0.0 6 8 183 3 0.0 6 3 184 6 0.0 6 6 185 3 0.0 6 3 186 5 0.0 6 5 187 4 0.0 6 4 188 6 0.0 6 6 189 4 0.0 6 4 190 6 0.0 6 6 191 5 0.0 6 5 192 6 0.0 6 6 193 1 0.0 6 1 194 2 0.0 6 2 195 5 0.0 6 5 196 4 0.0 6 4 197 6 0.0 6 6 198 2 0.0 6 2 199 2 0.0 6 2 200 3 0.0 6 3 201 4 0.0 6 4 202 1 0.0 6 1 203 3 0.0 6 3 205 4 0.0 6 4 206 1 0.0 6 1 207 2 0.0 6 2 208 1 0.0 6 1 210 1 0.0 6 1 211 2 0.0 6 2 212 1 0.0 6 1 213 3 0.0 6 3 214 2 0.0 6 2 215 4 0.0 6 4 216 1 0.0 6 1 217 2 0.0 6 2 219 1 0.0 6 1 221 3 0.0 6 3 222 1 0.0 6 1 226 1 0.0 6 1 229 1 0.0 6 1 241 1 0.0 6 1 247 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_15_30_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_15_257_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_15_30_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_15_257_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.62 s (22 µs/read; 2.70 M reads/minute). === Summary === Total read pairs processed: 72,809 Read 1 with adapter: 72,797 (100.0%) Read 2 with adapter: 72,757 (99.9%) == Read fate breakdown == Pairs that were too short: 39 (0.1%) Pairs discarded as untrimmed: 64 (0.1%) Pairs written (passing filters): 72,706 (99.9%) Total basepairs processed: 31,162,613 bp Read 1: 17,106,199 bp Read 2: 14,056,414 bp Total written (filtered): 4,953,899 bp (15.9%) Read 1: 2,550,541 bp Read 2: 2,403,358 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 70056 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1137.6 0 0 1 4 2 284.4 1 1 1 5 1 71.1 1 1 6 1 17.8 1 0 0 1 8 2 1.1 2 2 9 1 0.3 2 1 11 1 0.0 3 1 13 1 0.0 3 1 14 1 0.0 4 0 1 15 1 0.0 4 1 16 1 0.0 4 0 1 17 4 0.0 5 3 1 18 1 0.0 5 0 0 1 19 1 0.0 5 1 20 136 0.0 6 132 4 21 66 0.0 6 66 22 40 0.0 6 40 23 38 0.0 6 36 2 24 21 0.0 6 18 3 25 23 0.0 6 22 1 26 24 0.0 6 23 1 27 9 0.0 6 9 28 6 0.0 6 5 1 29 10 0.0 6 10 30 6 0.0 6 6 31 10 0.0 6 9 0 0 0 0 1 32 7 0.0 6 7 33 5 0.0 6 4 1 34 3 0.0 6 3 35 5 0.0 6 5 36 7 0.0 6 6 1 37 10 0.0 6 10 38 8 0.0 6 7 1 39 10 0.0 6 10 40 8 0.0 6 8 41 10 0.0 6 10 42 8 0.0 6 7 0 0 1 43 3 0.0 6 3 44 6 0.0 6 6 45 8 0.0 6 7 1 46 8 0.0 6 8 47 3 0.0 6 3 48 7 0.0 6 6 1 49 10 0.0 6 9 1 50 2 0.0 6 2 51 1 0.0 6 0 1 52 8 0.0 6 8 53 3 0.0 6 3 54 6 0.0 6 5 0 1 55 8 0.0 6 8 56 8 0.0 6 7 1 57 5 0.0 6 4 1 58 8 0.0 6 8 59 3 0.0 6 3 60 4 0.0 6 4 61 9 0.0 6 8 1 62 3 0.0 6 3 63 10 0.0 6 9 0 0 0 0 0 1 64 3 0.0 6 3 65 7 0.0 6 7 66 8 0.0 6 8 67 5 0.0 6 4 0 0 0 0 0 1 68 7 0.0 6 7 69 10 0.0 6 9 1 70 7 0.0 6 5 2 71 16 0.0 6 15 1 72 7 0.0 6 6 0 0 0 0 1 73 15 0.0 6 14 1 74 9 0.0 6 9 75 14 0.0 6 12 2 76 7 0.0 6 7 77 7 0.0 6 7 78 12 0.0 6 11 1 79 10 0.0 6 10 80 3 0.0 6 3 81 7 0.0 6 7 82 12 0.0 6 11 1 83 9 0.0 6 9 84 12 0.0 6 12 85 11 0.0 6 11 86 7 0.0 6 7 87 6 0.0 6 6 88 10 0.0 6 8 1 0 0 0 0 1 89 8 0.0 6 8 90 10 0.0 6 10 91 5 0.0 6 4 1 92 9 0.0 6 6 2 0 0 0 1 93 5 0.0 6 5 94 5 0.0 6 5 95 6 0.0 6 5 1 96 10 0.0 6 9 0 1 97 10 0.0 6 9 0 1 98 7 0.0 6 7 99 14 0.0 6 13 0 0 0 0 0 1 100 13 0.0 6 12 1 101 8 0.0 6 8 102 9 0.0 6 9 103 19 0.0 6 19 104 10 0.0 6 10 105 14 0.0 6 13 1 106 14 0.0 6 13 1 107 11 0.0 6 10 1 108 9 0.0 6 9 109 19 0.0 6 19 110 19 0.0 6 19 111 15 0.0 6 15 112 16 0.0 6 16 113 14 0.0 6 14 114 27 0.0 6 27 115 21 0.0 6 20 0 1 116 28 0.0 6 28 117 19 0.0 6 18 1 118 26 0.0 6 26 119 16 0.0 6 15 1 120 20 0.0 6 20 121 26 0.0 6 26 122 26 0.0 6 25 1 123 26 0.0 6 25 1 124 32 0.0 6 32 125 17 0.0 6 17 126 28 0.0 6 28 127 29 0.0 6 28 1 128 35 0.0 6 33 1 1 129 40 0.0 6 39 1 130 33 0.0 6 32 1 131 28 0.0 6 28 132 43 0.0 6 43 133 45 0.0 6 45 134 33 0.0 6 33 135 28 0.0 6 28 136 29 0.0 6 28 1 137 36 0.0 6 36 138 30 0.0 6 30 139 60 0.0 6 60 140 44 0.0 6 43 1 141 53 0.0 6 53 142 73 0.0 6 72 1 143 247 0.0 6 244 3 144 416 0.0 6 415 1 145 460 0.0 6 459 0 0 1 146 353 0.0 6 349 4 147 348 0.0 6 346 0 0 1 0 0 1 148 478 0.0 6 478 149 441 0.0 6 441 150 360 0.0 6 359 1 151 659 0.0 6 658 1 152 376 0.0 6 374 2 153 443 0.0 6 442 1 154 314 0.0 6 314 155 327 0.0 6 326 1 156 377 0.0 6 377 157 473 0.0 6 472 1 158 364 0.0 6 363 1 159 344 0.0 6 344 160 352 0.0 6 351 1 161 492 0.0 6 490 2 162 329 0.0 6 328 1 163 416 0.0 6 415 1 164 461 0.0 6 458 3 165 469 0.0 6 467 0 0 0 0 0 2 166 629 0.0 6 627 2 167 377 0.0 6 377 168 321 0.0 6 320 0 0 0 0 1 169 405 0.0 6 404 1 170 424 0.0 6 422 1 0 0 0 0 1 171 380 0.0 6 378 2 172 403 0.0 6 403 173 496 0.0 6 495 1 174 582 0.0 6 581 0 1 175 418 0.0 6 417 1 176 405 0.0 6 402 3 177 350 0.0 6 349 1 178 422 0.0 6 421 1 179 377 0.0 6 377 180 509 0.0 6 505 3 0 0 0 1 181 1131 0.0 6 1126 4 0 1 182 751 0.0 6 751 183 449 0.0 6 447 1 1 184 540 0.0 6 538 1 0 0 1 185 504 0.0 6 504 186 507 0.0 6 503 4 187 511 0.0 6 511 188 393 0.0 6 391 2 189 553 0.0 6 552 0 0 0 0 0 1 190 737 0.0 6 737 191 487 0.0 6 483 3 0 0 1 192 740 0.0 6 736 4 193 601 0.0 6 599 1 1 194 717 0.0 6 713 4 195 976 0.0 6 973 1 1 0 0 1 196 871 0.0 6 869 2 197 939 0.0 6 934 3 0 0 0 1 1 198 792 0.0 6 788 4 199 835 0.0 6 832 2 0 0 0 1 200 738 0.0 6 736 2 201 624 0.0 6 622 2 202 713 0.0 6 711 1 0 0 0 1 203 755 0.0 6 754 1 204 897 0.0 6 894 3 205 1165 0.0 6 1163 2 206 768 0.0 6 763 5 207 893 0.0 6 885 8 208 775 0.0 6 772 3 209 773 0.0 6 771 2 210 920 0.0 6 917 3 211 866 0.0 6 863 3 212 790 0.0 6 786 4 213 895 0.0 6 893 2 214 1124 0.0 6 1120 3 0 0 0 0 1 215 878 0.0 6 874 4 216 853 0.0 6 851 2 217 840 0.0 6 840 218 716 0.0 6 715 0 0 0 1 219 654 0.0 6 654 220 724 0.0 6 721 3 221 678 0.0 6 676 2 222 645 0.0 6 641 3 1 223 740 0.0 6 735 5 224 701 0.0 6 695 6 225 761 0.0 6 759 2 226 615 0.0 6 613 2 227 602 0.0 6 600 2 228 574 0.0 6 573 0 0 1 229 620 0.0 6 618 0 1 1 230 668 0.0 6 665 3 231 571 0.0 6 569 2 232 537 0.0 6 534 1 1 0 0 0 1 233 551 0.0 6 547 4 234 478 0.0 6 477 1 235 477 0.0 6 475 1 1 236 519 0.0 6 516 2 0 0 0 1 237 467 0.0 6 463 2 0 0 0 2 238 539 0.0 6 538 1 239 508 0.0 6 506 2 240 532 0.0 6 532 241 448 0.0 6 447 1 242 406 0.0 6 405 1 243 372 0.0 6 370 2 244 276 0.0 6 275 1 245 301 0.0 6 300 0 1 246 311 0.0 6 309 2 247 283 0.0 6 283 248 298 0.0 6 297 1 249 257 0.0 6 255 1 0 0 1 250 234 0.0 6 232 0 1 0 0 0 1 251 210 0.0 6 209 1 252 208 0.0 6 207 1 253 213 0.0 6 213 254 207 0.0 6 206 1 255 185 0.0 6 185 256 193 0.0 6 191 1 0 0 1 257 213 0.0 6 212 1 258 262 0.0 6 262 259 217 0.0 6 213 3 0 0 1 260 156 0.0 6 156 261 153 0.0 6 152 1 262 162 0.0 6 161 1 263 163 0.0 6 162 1 264 145 0.0 6 144 1 265 129 0.0 6 129 266 138 0.0 6 137 0 0 0 0 1 267 147 0.0 6 147 268 173 0.0 6 172 1 269 181 0.0 6 178 3 270 122 0.0 6 121 1 271 111 0.0 6 109 2 272 148 0.0 6 147 1 273 182 0.0 6 182 274 161 0.0 6 158 3 275 243 0.0 6 241 2 276 305 0.0 6 301 2 1 0 0 0 1 277 879 0.0 6 872 7 278 1204 0.0 6 1190 14 279 21 0.0 6 20 1 282 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2741 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 57 5 0.0 6 5 61 2 0.0 6 2 62 43 0.0 6 40 3 63 15 0.0 6 5 9 1 64 524 0.0 6 505 19 65 2 0.0 6 2 72 5 0.0 6 5 73 8 0.0 6 8 74 14 0.0 6 14 75 13 0.0 6 13 76 15 0.0 6 15 77 7 0.0 6 7 78 16 0.0 6 15 1 79 22 0.0 6 22 80 22 0.0 6 22 81 31 0.0 6 31 82 15 0.0 6 15 83 13 0.0 6 13 84 9 0.0 6 9 85 11 0.0 6 11 86 9 0.0 6 9 87 11 0.0 6 11 88 10 0.0 6 10 89 5 0.0 6 5 90 11 0.0 6 11 91 12 0.0 6 12 92 19 0.0 6 18 1 93 19 0.0 6 19 94 14 0.0 6 14 95 13 0.0 6 13 96 12 0.0 6 12 97 9 0.0 6 9 98 12 0.0 6 12 99 13 0.0 6 13 100 8 0.0 6 8 101 13 0.0 6 13 102 15 0.0 6 15 103 24 0.0 6 24 104 14 0.0 6 14 105 13 0.0 6 13 106 14 0.0 6 14 107 12 0.0 6 12 108 14 0.0 6 14 109 7 0.0 6 7 110 23 0.0 6 23 111 46 0.0 6 45 0 0 0 0 0 1 112 15 0.0 6 15 113 13 0.0 6 13 114 7 0.0 6 7 115 10 0.0 6 10 116 7 0.0 6 7 117 9 0.0 6 9 118 14 0.0 6 14 119 21 0.0 6 21 120 7 0.0 6 7 121 15 0.0 6 15 122 11 0.0 6 11 123 11 0.0 6 11 124 14 0.0 6 14 125 15 0.0 6 15 126 9 0.0 6 9 127 12 0.0 6 12 128 13 0.0 6 13 129 15 0.0 6 15 130 13 0.0 6 13 131 14 0.0 6 14 132 15 0.0 6 15 133 25 0.0 6 25 134 20 0.0 6 20 135 24 0.0 6 24 136 10 0.0 6 10 137 13 0.0 6 13 138 20 0.0 6 20 139 20 0.0 6 20 140 21 0.0 6 21 141 20 0.0 6 20 142 12 0.0 6 12 143 15 0.0 6 15 144 16 0.0 6 16 145 14 0.0 6 14 146 24 0.0 6 24 147 20 0.0 6 19 1 148 10 0.0 6 10 149 16 0.0 6 16 150 13 0.0 6 13 151 13 0.0 6 12 1 152 17 0.0 6 17 153 20 0.0 6 20 154 26 0.0 6 26 155 12 0.0 6 12 156 19 0.0 6 19 157 16 0.0 6 16 158 12 0.0 6 12 159 15 0.0 6 15 160 20 0.0 6 20 161 17 0.0 6 17 162 18 0.0 6 18 163 13 0.0 6 13 164 13 0.0 6 13 165 15 0.0 6 15 166 23 0.0 6 23 167 14 0.0 6 14 168 19 0.0 6 19 169 27 0.0 6 27 170 25 0.0 6 25 171 8 0.0 6 8 172 9 0.0 6 9 173 7 0.0 6 7 174 14 0.0 6 14 175 7 0.0 6 7 176 18 0.0 6 18 177 14 0.0 6 14 178 16 0.0 6 16 179 7 0.0 6 7 180 9 0.0 6 9 181 6 0.0 6 6 182 10 0.0 6 10 183 15 0.0 6 15 184 14 0.0 6 14 185 11 0.0 6 10 1 186 21 0.0 6 21 187 10 0.0 6 9 1 188 13 0.0 6 13 189 5 0.0 6 5 190 7 0.0 6 6 1 191 12 0.0 6 12 192 7 0.0 6 7 193 16 0.0 6 16 194 7 0.0 6 7 195 10 0.0 6 10 196 3 0.0 6 3 197 8 0.0 6 8 198 6 0.0 6 6 199 6 0.0 6 6 200 10 0.0 6 10 201 7 0.0 6 7 202 6 0.0 6 6 203 12 0.0 6 12 204 10 0.0 6 10 205 12 0.0 6 12 206 8 0.0 6 8 207 10 0.0 6 10 208 3 0.0 6 3 209 3 0.0 6 3 210 7 0.0 6 7 211 9 0.0 6 9 212 9 0.0 6 9 213 7 0.0 6 7 214 5 0.0 6 5 215 6 0.0 6 6 216 9 0.0 6 9 217 6 0.0 6 6 218 7 0.0 6 7 219 7 0.0 6 7 220 4 0.0 6 4 221 1 0.0 6 1 222 3 0.0 6 3 223 4 0.0 6 4 224 4 0.0 6 4 225 6 0.0 6 6 226 4 0.0 6 4 227 6 0.0 6 6 228 3 0.0 6 3 229 3 0.0 6 3 230 4 0.0 6 4 231 6 0.0 6 6 232 5 0.0 6 5 233 5 0.0 6 5 234 6 0.0 6 6 235 10 0.0 6 10 236 3 0.0 6 3 237 3 0.0 6 3 238 4 0.0 6 4 239 5 0.0 6 5 240 7 0.0 6 7 241 2 0.0 6 2 242 3 0.0 6 3 243 7 0.0 6 7 244 7 0.0 6 7 245 3 0.0 6 3 246 1 0.0 6 1 247 3 0.0 6 3 248 2 0.0 6 2 249 1 0.0 6 1 250 3 0.0 6 3 251 2 0.0 6 2 252 5 0.0 6 5 253 2 0.0 6 2 254 3 0.0 6 3 255 3 0.0 6 3 256 1 0.0 6 1 257 4 0.0 6 4 258 1 0.0 6 1 259 2 0.0 6 2 260 2 0.0 6 2 261 4 0.0 6 4 262 4 0.0 6 4 264 3 0.0 6 3 265 1 0.0 6 1 266 3 0.0 6 3 267 1 0.0 6 1 268 4 0.0 6 4 269 1 0.0 6 1 270 2 0.0 6 2 271 1 0.0 6 1 272 2 0.0 6 2 274 3 0.0 6 3 275 1 0.0 6 1 276 1 0.0 6 1 278 1 0.0 6 1 280 1 0.0 6 1 281 2 0.0 6 2 284 1 0.0 6 1 285 3 0.0 6 3 288 2 0.0 6 2 289 1 0.0 6 1 296 3 0.0 6 3 300 1 0.0 6 1 306 1 0.0 6 1 309 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 70475 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1137.6 0 0 1 6 2 17.8 1 1 1 7 1 4.4 2 0 1 8 1 1.1 2 0 1 9 1 0.3 2 1 10 3 0.1 3 2 1 11 5 0.0 3 5 12 2 0.0 3 2 13 8 0.0 3 8 14 2 0.0 4 2 15 2 0.0 4 1 0 0 0 0 0 1 16 15 0.0 4 14 1 17 20 0.0 5 16 4 18 16 0.0 5 14 1 1 19 13 0.0 5 10 3 20 118 0.0 6 113 5 21 46 0.0 6 42 4 22 28 0.0 6 28 23 36 0.0 6 35 1 24 27 0.0 6 25 2 25 20 0.0 6 18 2 26 24 0.0 6 22 2 27 15 0.0 6 13 1 0 0 0 0 1 28 25 0.0 6 21 3 0 1 29 9 0.0 6 9 30 15 0.0 6 15 31 13 0.0 6 10 3 32 7 0.0 6 5 1 1 33 10 0.0 6 9 1 34 15 0.0 6 13 2 35 16 0.0 6 16 36 9 0.0 6 7 0 1 0 0 1 37 10 0.0 6 10 38 17 0.0 6 12 4 1 39 11 0.0 6 11 40 10 0.0 6 10 41 9 0.0 6 8 1 42 8 0.0 6 7 1 43 12 0.0 6 12 44 7 0.0 6 6 1 45 21 0.0 6 19 2 46 10 0.0 6 9 0 0 1 47 18 0.0 6 17 1 48 13 0.0 6 12 1 49 8 0.0 6 6 2 50 11 0.0 6 11 51 18 0.0 6 17 0 0 1 52 11 0.0 6 11 53 24 0.0 6 19 5 54 19 0.0 6 17 2 55 23 0.0 6 21 1 0 1 56 22 0.0 6 18 3 0 0 0 0 1 57 21 0.0 6 19 2 58 27 0.0 6 26 1 59 17 0.0 6 17 60 23 0.0 6 20 2 1 61 18 0.0 6 15 3 62 19 0.0 6 17 2 63 33 0.0 6 29 4 64 28 0.0 6 26 2 65 29 0.0 6 27 1 0 1 66 42 0.0 6 39 2 1 67 146 0.0 6 139 5 2 68 49 0.0 6 46 2 0 1 69 75 0.0 6 70 4 0 1 70 26 0.0 6 25 1 71 38 0.0 6 38 72 36 0.0 6 35 1 73 32 0.0 6 31 1 74 44 0.0 6 43 1 75 31 0.0 6 29 2 76 25 0.0 6 23 1 1 77 36 0.0 6 33 3 78 35 0.0 6 33 2 79 31 0.0 6 27 3 0 0 0 1 80 35 0.0 6 33 1 1 81 62 0.0 6 58 4 82 48 0.0 6 46 2 83 61 0.0 6 58 3 84 40 0.0 6 38 1 0 0 0 1 85 23 0.0 6 23 86 43 0.0 6 42 1 87 36 0.0 6 31 3 1 0 0 0 1 88 39 0.0 6 38 1 89 37 0.0 6 37 90 54 0.0 6 49 4 1 91 54 0.0 6 48 6 92 49 0.0 6 44 5 93 47 0.0 6 44 2 1 94 68 0.0 6 65 2 0 1 95 60 0.0 6 57 1 0 2 96 73 0.0 6 69 2 0 1 0 1 97 65 0.0 6 57 6 1 1 98 80 0.0 6 78 2 99 57 0.0 6 55 2 100 47 0.0 6 43 4 101 58 0.0 6 53 5 102 67 0.0 6 63 3 0 1 103 75 0.0 6 74 1 104 142 0.0 6 136 5 0 0 0 0 1 105 74 0.0 6 73 0 0 0 0 0 1 106 75 0.0 6 71 3 1 107 101 0.0 6 98 3 108 81 0.0 6 80 1 109 85 0.0 6 85 110 95 0.0 6 92 3 111 90 0.0 6 90 112 108 0.0 6 104 3 0 0 1 113 85 0.0 6 85 114 114 0.0 6 111 3 115 127 0.0 6 122 4 0 0 1 116 118 0.0 6 114 3 1 117 136 0.0 6 132 4 118 146 0.0 6 144 1 0 0 1 119 125 0.0 6 122 2 0 1 120 150 0.0 6 149 1 121 138 0.0 6 135 3 122 134 0.0 6 130 4 123 149 0.0 6 144 4 0 1 124 197 0.0 6 189 8 125 210 0.0 6 204 3 1 1 0 0 1 126 200 0.0 6 196 4 127 232 0.0 6 227 5 128 265 0.0 6 259 6 129 254 0.0 6 248 5 0 1 130 286 0.0 6 278 4 2 0 1 0 1 131 366 0.0 6 352 14 132 689 0.0 6 669 17 2 0 0 0 1 133 412 0.0 6 401 8 1 0 0 1 1 134 357 0.0 6 351 4 0 0 0 0 2 135 349 0.0 6 342 5 2 136 363 0.0 6 359 3 0 1 137 384 0.0 6 378 4 0 0 1 1 138 404 0.0 6 399 3 1 0 1 139 405 0.0 6 393 12 140 470 0.0 6 466 4 141 624 0.0 6 617 7 142 856 0.0 6 845 9 1 0 0 1 143 780 0.0 6 773 6 1 144 822 0.0 6 811 10 0 0 1 145 859 0.0 6 845 7 2 1 2 2 146 1015 0.0 6 1009 6 147 915 0.0 6 905 9 0 1 148 1085 0.0 6 1077 5 0 0 1 0 2 149 1317 0.0 6 1311 6 150 1468 0.0 6 1444 20 2 0 1 0 1 151 1166 0.0 6 1149 15 1 0 1 152 1304 0.0 6 1293 10 0 1 153 1354 0.0 6 1336 16 1 0 0 1 154 1112 0.0 6 1100 9 1 1 1 155 1133 0.0 6 1119 11 1 0 0 1 1 156 1115 0.0 6 1102 13 157 1199 0.0 6 1186 9 2 0 0 1 1 158 1247 0.0 6 1228 17 0 0 2 159 1153 0.0 6 1145 6 0 0 1 0 1 160 1145 0.0 6 1131 12 0 1 0 1 161 1119 0.0 6 1105 11 1 0 0 2 162 1024 0.0 6 1014 8 1 0 0 1 163 1084 0.0 6 1075 7 2 164 1023 0.0 6 1014 7 0 0 1 0 1 165 871 0.0 6 859 11 1 166 1157 0.0 6 1144 11 0 2 167 983 0.0 6 973 9 0 0 1 168 1183 0.0 6 1177 6 169 1057 0.0 6 1053 4 170 1020 0.0 6 1013 7 171 1206 0.0 6 1201 5 172 1154 0.0 6 1146 8 173 1141 0.0 6 1134 7 174 1070 0.0 6 1063 6 0 0 0 1 175 1123 0.0 6 1116 6 1 176 1179 0.0 6 1173 5 0 1 177 954 0.0 6 947 6 0 0 1 178 989 0.0 6 979 8 1 0 1 179 899 0.0 6 894 4 0 0 1 180 822 0.0 6 817 4 1 181 736 0.0 6 732 3 0 1 182 677 0.0 6 673 4 183 961 0.0 6 950 9 0 1 0 0 1 184 1144 0.0 6 1141 3 185 1041 0.0 6 1035 3 0 0 2 0 1 186 781 0.0 6 775 4 1 1 187 759 0.0 6 754 3 0 0 0 1 1 188 745 0.0 6 736 8 1 189 703 0.0 6 700 3 190 646 0.0 6 641 4 0 0 1 191 602 0.0 6 600 1 0 0 0 0 1 192 502 0.0 6 499 3 193 489 0.0 6 486 2 0 0 0 1 194 409 0.0 6 404 5 195 402 0.0 6 395 7 196 566 0.0 6 565 1 197 397 0.0 6 394 3 198 404 0.0 6 401 3 199 329 0.0 6 325 4 200 330 0.0 6 327 3 201 277 0.0 6 276 1 202 286 0.0 6 284 2 203 248 0.0 6 247 1 204 237 0.0 6 235 2 205 220 0.0 6 218 2 206 230 0.0 6 228 1 1 207 202 0.0 6 199 2 0 0 0 1 208 175 0.0 6 175 209 168 0.0 6 166 2 210 131 0.0 6 129 1 0 0 0 1 211 153 0.0 6 151 2 212 151 0.0 6 150 1 213 153 0.0 6 151 2 214 168 0.0 6 168 215 116 0.0 6 115 1 216 122 0.0 6 121 0 0 0 0 0 1 217 108 0.0 6 107 1 218 105 0.0 6 104 1 219 80 0.0 6 78 2 220 49 0.0 6 48 1 221 99 0.0 6 99 222 57 0.0 6 56 1 223 54 0.0 6 52 2 224 32 0.0 6 32 225 41 0.0 6 41 226 35 0.0 6 34 0 0 0 0 0 1 227 33 0.0 6 33 228 26 0.0 6 25 1 229 30 0.0 6 30 230 23 0.0 6 23 231 19 0.0 6 18 1 232 23 0.0 6 23 233 16 0.0 6 16 234 15 0.0 6 15 235 12 0.0 6 11 1 236 8 0.0 6 8 237 5 0.0 6 5 238 6 0.0 6 6 239 5 0.0 6 5 240 4 0.0 6 4 241 9 0.0 6 9 242 7 0.0 6 7 243 2 0.0 6 2 244 6 0.0 6 6 245 1 0.0 6 1 246 1 0.0 6 1 247 4 0.0 6 4 248 3 0.0 6 3 249 4 0.0 6 4 250 2 0.0 6 2 251 3 0.0 6 3 252 1 0.0 6 1 253 2 0.0 6 2 254 1 0.0 6 1 256 1 0.0 6 1 277 3 0.0 6 3 278 8 0.0 6 8 279 82 0.0 6 81 1 280 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2282 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 56 1 0.0 6 1 66 1 0.0 6 1 71 5 0.0 6 5 72 37 0.0 6 35 2 73 16 0.0 6 16 74 13 0.0 6 13 75 23 0.0 6 22 1 76 10 0.0 6 10 77 15 0.0 6 15 78 24 0.0 6 24 79 13 0.0 6 13 80 34 0.0 6 34 81 9 0.0 6 8 1 82 19 0.0 6 19 83 4 0.0 6 3 0 0 1 84 14 0.0 6 14 85 25 0.0 6 25 86 16 0.0 6 16 87 15 0.0 6 15 88 15 0.0 6 15 89 12 0.0 6 12 90 17 0.0 6 17 91 9 0.0 6 9 92 14 0.0 6 14 93 13 0.0 6 12 0 0 0 1 94 27 0.0 6 26 0 0 0 1 95 176 0.0 6 176 96 10 0.0 6 10 97 12 0.0 6 12 98 26 0.0 6 26 99 18 0.0 6 18 100 16 0.0 6 16 101 10 0.0 6 10 102 20 0.0 6 20 103 10 0.0 6 10 104 9 0.0 6 9 105 11 0.0 6 11 106 10 0.0 6 10 107 9 0.0 6 9 108 12 0.0 6 12 109 24 0.0 6 24 110 42 0.0 6 42 111 20 0.0 6 20 112 14 0.0 6 14 113 17 0.0 6 17 114 9 0.0 6 9 115 17 0.0 6 17 116 12 0.0 6 12 117 12 0.0 6 12 118 20 0.0 6 20 119 23 0.0 6 23 120 16 0.0 6 16 121 25 0.0 6 25 122 10 0.0 6 10 123 17 0.0 6 17 124 27 0.0 6 27 125 17 0.0 6 17 126 16 0.0 6 16 127 12 0.0 6 12 128 36 0.0 6 36 129 20 0.0 6 19 0 0 0 1 130 14 0.0 6 14 131 21 0.0 6 21 132 25 0.0 6 25 133 36 0.0 6 36 134 19 0.0 6 19 135 17 0.0 6 16 1 136 19 0.0 6 19 137 19 0.0 6 19 138 33 0.0 6 33 139 27 0.0 6 27 140 27 0.0 6 27 141 17 0.0 6 17 142 16 0.0 6 15 1 143 17 0.0 6 17 144 16 0.0 6 16 145 16 0.0 6 16 146 20 0.0 6 20 147 11 0.0 6 11 148 20 0.0 6 20 149 21 0.0 6 21 150 18 0.0 6 18 151 20 0.0 6 19 1 152 23 0.0 6 23 153 15 0.0 6 15 154 14 0.0 6 14 155 19 0.0 6 19 156 12 0.0 6 12 157 15 0.0 6 15 158 13 0.0 6 12 0 0 0 1 159 20 0.0 6 20 160 14 0.0 6 14 161 19 0.0 6 19 162 16 0.0 6 16 163 15 0.0 6 15 164 19 0.0 6 18 0 0 0 1 165 23 0.0 6 23 166 9 0.0 6 9 167 10 0.0 6 10 168 27 0.0 6 27 169 15 0.0 6 15 170 19 0.0 6 19 171 11 0.0 6 11 172 8 0.0 6 8 173 14 0.0 6 14 174 10 0.0 6 10 175 13 0.0 6 13 176 12 0.0 6 12 177 19 0.0 6 19 178 9 0.0 6 9 179 10 0.0 6 10 180 9 0.0 6 9 181 10 0.0 6 10 182 22 0.0 6 21 0 0 0 0 0 1 183 10 0.0 6 10 184 12 0.0 6 12 185 13 0.0 6 13 186 10 0.0 6 10 187 10 0.0 6 10 188 8 0.0 6 8 189 5 0.0 6 5 190 3 0.0 6 3 191 7 0.0 6 7 192 11 0.0 6 11 193 3 0.0 6 3 195 3 0.0 6 3 196 10 0.0 6 10 197 8 0.0 6 8 198 4 0.0 6 4 199 5 0.0 6 5 200 2 0.0 6 2 201 6 0.0 6 6 202 7 0.0 6 7 203 5 0.0 6 5 204 6 0.0 6 6 205 6 0.0 6 6 206 2 0.0 6 2 207 1 0.0 6 1 208 6 0.0 6 4 2 209 1 0.0 6 1 211 8 0.0 6 8 212 3 0.0 6 3 213 4 0.0 6 4 214 2 0.0 6 2 215 4 0.0 6 4 216 2 0.0 6 2 217 1 0.0 6 1 218 2 0.0 6 2 219 1 0.0 6 1 221 3 0.0 6 3 222 2 0.0 6 2 223 1 0.0 6 1 224 2 0.0 6 2 225 1 0.0 6 1 227 1 0.0 6 1 228 2 0.0 6 2 229 3 0.0 6 3 230 1 0.0 6 1 231 2 0.0 6 2 234 1 0.0 6 1 235 1 0.0 6 1 240 1 0.0 6 1 243 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_16_31_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_16_258_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_16_31_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_16_258_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.80 s (26 µs/read; 2.34 M reads/minute). === Summary === Total read pairs processed: 70,333 Read 1 with adapter: 70,327 (100.0%) Read 2 with adapter: 70,302 (100.0%) == Read fate breakdown == Pairs that were too short: 2 (0.0%) Pairs discarded as untrimmed: 36 (0.1%) Pairs written (passing filters): 70,295 (99.9%) Total basepairs processed: 34,428,600 bp Read 1: 19,496,892 bp Read 2: 14,931,708 bp Total written (filtered): 4,446,243 bp (12.9%) Read 1: 2,328,329 bp Read 2: 2,117,914 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 70162 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1099.0 0 1 1 6 2 17.2 1 0 0 2 7 3 4.3 2 2 0 1 9 3 0.3 2 3 15 2 0.0 4 2 16 1 0.0 4 1 17 2 0.0 5 2 19 1 0.0 5 0 1 20 30 0.0 6 30 21 6 0.0 6 6 22 7 0.0 6 6 0 1 23 1 0.0 6 1 24 1 0.0 6 1 25 15 0.0 6 15 26 4 0.0 6 4 27 7 0.0 6 7 28 4 0.0 6 4 29 2 0.0 6 1 1 30 3 0.0 6 2 0 1 31 5 0.0 6 5 32 3 0.0 6 2 0 1 33 2 0.0 6 2 34 1 0.0 6 1 35 1 0.0 6 0 1 36 2 0.0 6 2 37 3 0.0 6 3 38 4 0.0 6 4 39 1 0.0 6 1 40 6 0.0 6 4 2 42 4 0.0 6 4 43 1 0.0 6 0 1 44 2 0.0 6 1 1 45 4 0.0 6 4 46 7 0.0 6 7 47 3 0.0 6 3 48 1 0.0 6 1 49 3 0.0 6 3 50 2 0.0 6 2 51 2 0.0 6 2 52 4 0.0 6 4 53 3 0.0 6 2 1 54 1 0.0 6 1 55 2 0.0 6 2 56 4 0.0 6 4 57 5 0.0 6 4 1 59 5 0.0 6 5 60 1 0.0 6 1 61 1 0.0 6 1 62 5 0.0 6 5 63 3 0.0 6 3 64 11 0.0 6 11 65 3 0.0 6 3 66 2 0.0 6 2 67 3 0.0 6 3 68 4 0.0 6 3 1 69 2 0.0 6 2 71 1 0.0 6 1 72 2 0.0 6 2 73 2 0.0 6 2 74 3 0.0 6 3 75 3 0.0 6 3 76 1 0.0 6 1 77 1 0.0 6 0 1 78 3 0.0 6 2 1 79 2 0.0 6 2 80 4 0.0 6 3 1 81 4 0.0 6 4 82 2 0.0 6 2 83 2 0.0 6 2 84 3 0.0 6 3 85 4 0.0 6 4 86 10 0.0 6 10 87 1 0.0 6 1 89 5 0.0 6 5 90 2 0.0 6 2 91 1 0.0 6 1 92 6 0.0 6 5 0 1 93 2 0.0 6 0 2 94 1 0.0 6 1 96 1 0.0 6 0 0 1 97 6 0.0 6 6 98 4 0.0 6 3 1 99 3 0.0 6 3 100 3 0.0 6 3 101 15 0.0 6 15 102 7 0.0 6 7 103 4 0.0 6 4 104 1 0.0 6 1 105 3 0.0 6 3 106 6 0.0 6 5 1 107 7 0.0 6 7 108 10 0.0 6 10 109 6 0.0 6 6 110 1 0.0 6 1 111 6 0.0 6 6 112 9 0.0 6 9 113 10 0.0 6 10 114 2 0.0 6 2 115 5 0.0 6 5 116 5 0.0 6 5 117 10 0.0 6 9 1 118 5 0.0 6 5 119 10 0.0 6 9 0 1 120 9 0.0 6 8 1 121 10 0.0 6 10 122 18 0.0 6 17 1 123 19 0.0 6 18 0 1 124 9 0.0 6 8 0 1 125 19 0.0 6 18 1 126 9 0.0 6 7 1 1 127 7 0.0 6 7 128 13 0.0 6 13 129 5 0.0 6 5 130 7 0.0 6 7 131 6 0.0 6 6 132 2 0.0 6 2 133 14 0.0 6 14 134 25 0.0 6 25 135 26 0.0 6 25 1 136 9 0.0 6 9 137 54 0.0 6 53 1 138 58 0.0 6 55 3 139 33 0.0 6 31 2 140 58 0.0 6 56 2 141 111 0.0 6 110 1 142 118 0.0 6 117 1 143 117 0.0 6 115 2 144 147 0.0 6 146 1 145 218 0.0 6 217 1 146 94 0.0 6 93 1 147 102 0.0 6 101 1 148 142 0.0 6 138 4 149 108 0.0 6 103 5 150 165 0.0 6 163 2 151 183 0.0 6 180 2 1 152 126 0.0 6 123 3 153 108 0.0 6 107 1 154 152 0.0 6 152 155 178 0.0 6 178 156 123 0.0 6 122 1 157 101 0.0 6 100 1 158 118 0.0 6 118 159 85 0.0 6 85 160 100 0.0 6 98 1 1 161 166 0.0 6 163 3 162 70 0.0 6 69 1 163 145 0.0 6 144 1 164 132 0.0 6 132 165 84 0.0 6 82 2 166 49 0.0 6 46 3 167 92 0.0 6 92 168 147 0.0 6 146 1 169 123 0.0 6 122 1 170 187 0.0 6 187 171 114 0.0 6 111 3 172 109 0.0 6 107 2 173 121 0.0 6 117 4 174 132 0.0 6 130 1 1 175 165 0.0 6 160 3 2 176 120 0.0 6 120 177 106 0.0 6 102 4 178 73 0.0 6 72 1 179 99 0.0 6 98 1 180 110 0.0 6 107 3 181 167 0.0 6 164 3 182 143 0.0 6 136 7 183 138 0.0 6 135 3 184 164 0.0 6 161 3 185 253 0.0 6 250 3 186 368 0.0 6 363 5 187 152 0.0 6 148 4 188 103 0.0 6 101 2 189 113 0.0 6 110 3 190 149 0.0 6 143 6 191 152 0.0 6 147 5 192 170 0.0 6 161 8 1 193 274 0.0 6 264 9 1 194 226 0.0 6 219 7 195 311 0.0 6 301 10 196 358 0.0 6 352 6 197 279 0.0 6 269 10 198 282 0.0 6 277 5 199 171 0.0 6 170 1 200 204 0.0 6 199 3 1 0 1 201 273 0.0 6 270 3 202 264 0.0 6 251 12 1 203 345 0.0 6 337 7 1 204 245 0.0 6 239 5 1 205 377 0.0 6 365 11 0 1 206 335 0.0 6 325 10 207 399 0.0 6 392 6 1 208 336 0.0 6 326 10 209 323 0.0 6 315 8 210 270 0.0 6 268 2 211 211 0.0 6 209 2 212 171 0.0 6 169 2 213 263 0.0 6 257 6 214 351 0.0 6 347 4 215 201 0.0 6 193 8 216 245 0.0 6 240 4 1 217 422 0.0 6 410 10 2 218 348 0.0 6 345 3 219 278 0.0 6 276 2 220 307 0.0 6 297 9 0 0 1 221 218 0.0 6 205 13 222 420 0.0 6 409 11 223 223 0.0 6 219 4 224 256 0.0 6 249 6 0 0 1 225 333 0.0 6 323 9 1 226 824 0.0 6 807 16 0 0 0 0 1 227 712 0.0 6 691 20 1 228 595 0.0 6 579 16 229 511 0.0 6 497 14 230 512 0.0 6 500 11 1 231 337 0.0 6 325 10 2 232 468 0.0 6 461 7 233 526 0.0 6 511 15 234 460 0.0 6 452 7 1 235 317 0.0 6 311 5 1 236 277 0.0 6 270 6 1 237 222 0.0 6 212 9 1 238 379 0.0 6 368 10 1 239 404 0.0 6 391 12 1 240 454 0.0 6 441 11 2 241 683 0.0 6 668 13 2 242 655 0.0 6 641 14 243 649 0.0 6 637 12 244 387 0.0 6 376 11 245 685 0.0 6 671 13 0 1 246 309 0.0 6 303 6 247 396 0.0 6 383 13 248 194 0.0 6 191 3 249 717 0.0 6 694 23 250 387 0.0 6 372 14 1 251 592 0.0 6 577 14 0 1 252 413 0.0 6 408 4 1 253 316 0.0 6 300 16 254 781 0.0 6 761 20 255 461 0.0 6 446 15 256 424 0.0 6 409 15 257 636 0.0 6 620 16 258 622 0.0 6 599 23 259 947 0.0 6 918 29 260 974 0.0 6 950 24 261 410 0.0 6 396 14 262 842 0.0 6 816 25 1 263 1611 0.0 6 1582 27 2 264 917 0.0 6 893 24 265 1179 0.0 6 1159 20 266 668 0.0 6 647 19 2 267 997 0.0 6 974 22 1 268 1008 0.0 6 967 41 269 733 0.0 6 712 20 0 1 270 297 0.0 6 283 12 2 271 895 0.0 6 869 26 272 2502 0.0 6 2447 54 0 1 273 1186 0.0 6 1152 31 3 274 1555 0.0 6 1513 42 275 1392 0.0 6 1355 36 1 276 1949 0.0 6 1888 59 2 277 6016 0.0 6 5876 133 6 1 278 10264 0.0 6 10068 180 14 2 279 131 0.0 6 115 14 0 1 1 280 23 0.0 6 22 1 281 4 0.0 6 4 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 165 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 64 4 0.0 6 4 73 1 0.0 6 1 74 1 0.0 6 1 75 2 0.0 6 2 76 8 0.0 6 8 77 4 0.0 6 4 79 6 0.0 6 6 80 2 0.0 6 2 81 2 0.0 6 2 82 5 0.0 6 5 83 4 0.0 6 4 84 2 0.0 6 2 85 1 0.0 6 1 86 5 0.0 6 5 87 8 0.0 6 8 88 4 0.0 6 4 90 1 0.0 6 1 91 4 0.0 6 4 92 1 0.0 6 1 93 2 0.0 6 2 94 1 0.0 6 1 95 2 0.0 6 2 97 2 0.0 6 2 99 3 0.0 6 3 100 2 0.0 6 2 101 6 0.0 6 6 102 4 0.0 6 4 103 3 0.0 6 3 104 4 0.0 6 4 105 5 0.0 6 5 106 2 0.0 6 2 108 2 0.0 6 2 109 1 0.0 6 1 110 1 0.0 6 1 112 3 0.0 6 3 115 1 0.0 6 1 116 1 0.0 6 1 117 3 0.0 6 3 118 2 0.0 6 2 119 1 0.0 6 1 121 1 0.0 6 1 123 1 0.0 6 1 125 2 0.0 6 2 128 1 0.0 6 1 129 1 0.0 6 1 133 2 0.0 6 2 134 1 0.0 6 1 135 1 0.0 6 1 138 1 0.0 6 1 144 1 0.0 6 1 146 1 0.0 6 1 149 1 0.0 6 1 151 1 0.0 6 1 152 1 0.0 6 1 154 1 0.0 6 1 155 3 0.0 6 3 156 1 0.0 6 1 158 1 0.0 6 1 159 1 0.0 6 1 160 1 0.0 6 1 163 2 0.0 6 2 164 1 0.0 6 1 170 1 0.0 6 1 171 3 0.0 6 3 172 1 0.0 6 1 173 2 0.0 6 2 174 1 0.0 6 1 182 1 0.0 6 1 183 1 0.0 6 1 188 1 0.0 6 1 189 1 0.0 6 1 194 1 0.0 6 1 200 1 0.0 6 1 201 1 0.0 6 1 211 1 0.0 6 1 213 1 0.0 6 1 214 1 0.0 6 1 219 1 0.0 6 1 229 1 0.0 6 1 230 1 0.0 6 1 233 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 70109 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 2 17.2 1 1 1 7 1 4.3 2 0 1 9 2 0.3 2 2 11 3 0.0 3 3 12 4 0.0 3 3 0 0 1 13 12 0.0 3 10 2 14 2 0.0 4 2 16 7 0.0 4 6 1 17 7 0.0 5 6 1 18 7 0.0 5 6 1 19 10 0.0 5 8 2 20 41 0.0 6 39 2 21 53 0.0 6 52 1 22 34 0.0 6 33 1 23 30 0.0 6 29 1 24 23 0.0 6 22 1 25 11 0.0 6 9 2 26 13 0.0 6 13 27 6 0.0 6 3 3 28 9 0.0 6 8 0 0 0 1 29 16 0.0 6 16 30 10 0.0 6 9 1 31 11 0.0 6 10 1 32 12 0.0 6 12 33 6 0.0 6 4 2 34 9 0.0 6 9 35 4 0.0 6 4 36 5 0.0 6 4 1 37 12 0.0 6 10 2 38 17 0.0 6 16 1 39 11 0.0 6 11 40 8 0.0 6 8 41 7 0.0 6 7 42 4 0.0 6 4 43 5 0.0 6 5 44 13 0.0 6 13 45 4 0.0 6 3 1 46 13 0.0 6 11 2 47 18 0.0 6 17 1 48 11 0.0 6 9 2 49 9 0.0 6 9 50 14 0.0 6 13 1 51 6 0.0 6 5 1 52 6 0.0 6 5 0 1 53 8 0.0 6 8 54 28 0.0 6 25 3 55 18 0.0 6 15 2 0 0 1 56 21 0.0 6 18 3 57 16 0.0 6 15 1 58 9 0.0 6 8 1 59 16 0.0 6 14 1 1 60 9 0.0 6 8 1 61 9 0.0 6 9 62 16 0.0 6 15 1 63 3 0.0 6 3 64 12 0.0 6 12 65 8 0.0 6 7 1 66 18 0.0 6 17 1 67 14 0.0 6 13 1 68 22 0.0 6 20 1 0 0 1 69 126 0.0 6 123 3 70 29 0.0 6 28 1 71 73 0.0 6 70 2 1 72 39 0.0 6 38 1 73 20 0.0 6 20 74 12 0.0 6 11 1 75 13 0.0 6 9 4 76 24 0.0 6 22 1 1 77 24 0.0 6 21 3 78 33 0.0 6 30 3 79 14 0.0 6 14 80 18 0.0 6 17 1 81 12 0.0 6 12 82 43 0.0 6 42 1 83 35 0.0 6 32 3 84 36 0.0 6 35 1 85 52 0.0 6 50 2 86 25 0.0 6 23 2 87 40 0.0 6 39 1 88 15 0.0 6 13 2 89 29 0.0 6 27 2 90 41 0.0 6 38 3 91 31 0.0 6 29 2 92 19 0.0 6 16 3 93 46 0.0 6 40 4 2 94 98 0.0 6 93 5 95 26 0.0 6 25 0 1 96 40 0.0 6 36 4 97 42 0.0 6 39 3 98 50 0.0 6 49 1 99 56 0.0 6 55 1 100 37 0.0 6 34 3 101 31 0.0 6 28 3 102 95 0.0 6 89 6 103 98 0.0 6 92 6 104 77 0.0 6 72 5 105 86 0.0 6 84 2 106 55 0.0 6 54 1 107 66 0.0 6 64 2 108 49 0.0 6 46 3 109 46 0.0 6 42 4 110 66 0.0 6 61 5 111 58 0.0 6 56 2 112 54 0.0 6 52 1 1 113 45 0.0 6 44 1 114 69 0.0 6 69 115 66 0.0 6 63 2 1 116 59 0.0 6 57 2 117 35 0.0 6 31 4 118 62 0.0 6 59 3 119 84 0.0 6 81 3 120 118 0.0 6 110 7 1 121 81 0.0 6 79 2 122 97 0.0 6 91 4 1 0 0 1 123 115 0.0 6 106 9 124 135 0.0 6 132 3 125 83 0.0 6 80 3 126 46 0.0 6 45 1 127 105 0.0 6 101 4 128 77 0.0 6 75 2 129 64 0.0 6 62 2 130 86 0.0 6 82 4 131 160 0.0 6 154 5 1 132 171 0.0 6 166 4 1 133 149 0.0 6 148 1 134 222 0.0 6 214 8 135 337 0.0 6 323 13 0 1 136 179 0.0 6 175 4 137 236 0.0 6 226 10 138 174 0.0 6 169 5 139 203 0.0 6 195 8 140 210 0.0 6 203 7 141 273 0.0 6 262 10 0 0 1 142 395 0.0 6 385 10 143 328 0.0 6 323 4 1 144 537 0.0 6 525 12 145 328 0.0 6 319 9 146 268 0.0 6 263 5 147 220 0.0 6 217 3 148 295 0.0 6 286 7 1 0 1 149 354 0.0 6 343 9 2 150 574 0.0 6 557 16 1 151 319 0.0 6 305 13 0 0 1 152 796 0.0 6 782 14 153 392 0.0 6 380 12 154 556 0.0 6 550 5 1 155 561 0.0 6 551 10 156 446 0.0 6 438 7 1 157 560 0.0 6 547 13 158 629 0.0 6 615 13 0 0 1 159 300 0.0 6 296 4 160 572 0.0 6 561 11 161 430 0.0 6 422 7 1 162 344 0.0 6 333 10 0 0 1 163 519 0.0 6 513 5 1 164 413 0.0 6 405 7 0 0 1 165 547 0.0 6 535 11 1 166 667 0.0 6 650 17 167 273 0.0 6 267 6 168 886 0.0 6 869 17 169 257 0.0 6 253 3 0 1 170 527 0.0 6 515 12 171 848 0.0 6 829 19 172 239 0.0 6 230 9 173 382 0.0 6 372 9 1 174 579 0.0 6 565 12 1 0 1 175 1067 0.0 6 1030 35 1 1 176 584 0.0 6 564 18 1 0 0 1 177 857 0.0 6 834 21 2 178 608 0.0 6 595 12 1 179 736 0.0 6 708 25 2 0 1 180 775 0.0 6 750 24 1 181 863 0.0 6 844 18 1 182 259 0.0 6 249 10 183 720 0.0 6 698 22 184 261 0.0 6 255 4 1 0 1 185 461 0.0 6 442 16 1 1 0 1 186 1727 0.0 6 1674 48 3 0 2 187 1388 0.0 6 1351 33 2 1 1 188 1322 0.0 6 1271 50 0 0 1 189 1284 0.0 6 1247 36 1 190 1957 0.0 6 1904 51 0 0 2 191 1452 0.0 6 1409 41 1 0 1 192 1100 0.0 6 1071 27 2 193 703 0.0 6 673 25 2 1 2 194 1186 0.0 6 1154 30 1 1 195 1649 0.0 6 1584 64 0 0 1 196 3318 0.0 6 3238 75 1 0 4 197 490 0.0 6 467 17 6 198 2417 0.0 6 2319 92 1 3 1 1 199 3845 0.0 6 3744 98 0 1 2 200 917 0.0 6 885 30 2 201 1182 0.0 6 1146 35 0 1 202 709 0.0 6 683 24 2 203 746 0.0 6 724 22 204 858 0.0 6 845 13 205 322 0.0 6 311 10 1 206 299 0.0 6 295 4 207 204 0.0 6 202 2 208 411 0.0 6 398 13 209 323 0.0 6 313 9 0 1 210 297 0.0 6 290 7 211 439 0.0 6 427 12 212 417 0.0 6 404 11 1 0 1 213 96 0.0 6 93 2 1 214 274 0.0 6 270 4 215 366 0.0 6 355 10 0 0 1 216 956 0.0 6 933 23 217 616 0.0 6 603 13 218 449 0.0 6 433 15 0 0 1 219 430 0.0 6 419 11 220 298 0.0 6 288 10 221 416 0.0 6 404 12 222 541 0.0 6 523 17 1 223 1397 0.0 6 1370 26 1 224 231 0.0 6 221 10 225 544 0.0 6 524 20 226 366 0.0 6 359 7 227 222 0.0 6 211 11 228 109 0.0 6 104 5 229 274 0.0 6 268 6 230 155 0.0 6 148 7 231 184 0.0 6 179 5 232 260 0.0 6 252 6 0 0 2 233 462 0.0 6 453 9 234 166 0.0 6 164 2 235 70 0.0 6 69 1 236 48 0.0 6 46 2 237 53 0.0 6 50 3 238 25 0.0 6 23 2 239 15 0.0 6 14 0 0 1 240 109 0.0 6 107 2 241 76 0.0 6 74 2 242 189 0.0 6 187 2 243 212 0.0 6 207 5 244 92 0.0 6 90 2 245 27 0.0 6 26 1 246 34 0.0 6 32 2 247 12 0.0 6 12 248 29 0.0 6 29 249 25 0.0 6 25 250 30 0.0 6 29 1 251 10 0.0 6 10 252 7 0.0 6 7 253 23 0.0 6 23 254 2 0.0 6 2 255 2 0.0 6 2 269 1 0.0 6 1 278 1 0.0 6 1 279 10 0.0 6 10 280 38 0.0 6 37 1 281 207 0.0 6 199 8 283 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 193 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 23 0.0 6 23 70 2 0.0 6 2 71 1 0.0 6 1 73 5 0.0 6 5 74 5 0.0 6 5 75 6 0.0 6 6 76 5 0.0 6 5 77 3 0.0 6 3 79 5 0.0 6 5 80 2 0.0 6 2 82 4 0.0 6 4 83 4 0.0 6 4 84 6 0.0 6 6 85 3 0.0 6 3 86 3 0.0 6 3 87 4 0.0 6 4 88 1 0.0 6 1 89 2 0.0 6 2 90 4 0.0 6 4 91 3 0.0 6 3 92 2 0.0 6 2 93 4 0.0 6 4 94 3 0.0 6 3 95 1 0.0 6 1 96 1 0.0 6 1 97 4 0.0 6 4 98 3 0.0 6 3 99 6 0.0 6 5 1 101 1 0.0 6 1 102 6 0.0 6 6 103 2 0.0 6 2 104 1 0.0 6 1 105 4 0.0 6 4 106 2 0.0 6 2 108 1 0.0 6 1 111 1 0.0 6 1 112 2 0.0 6 2 113 4 0.0 6 4 114 3 0.0 6 3 115 3 0.0 6 3 116 2 0.0 6 2 117 1 0.0 6 1 118 1 0.0 6 1 119 2 0.0 6 2 120 1 0.0 6 1 123 2 0.0 6 2 126 2 0.0 6 2 127 1 0.0 6 1 130 3 0.0 6 3 131 1 0.0 6 1 136 1 0.0 6 1 139 2 0.0 6 2 141 1 0.0 6 1 145 1 0.0 6 1 147 1 0.0 6 1 149 1 0.0 6 1 152 4 0.0 6 4 153 2 0.0 6 2 154 1 0.0 6 1 155 1 0.0 6 1 160 1 0.0 6 1 168 1 0.0 6 1 172 1 0.0 6 1 173 1 0.0 6 1 174 1 0.0 6 1 176 1 0.0 6 1 177 1 0.0 6 1 180 3 0.0 6 3 186 1 0.0 6 1 191 1 0.0 6 1 193 1 0.0 6 1 195 1 0.0 6 1 197 1 0.0 6 1 223 1 0.0 6 1 262 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_17_32_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_17_259_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_17_32_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_17_259_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.99 s (24 µs/read; 2.55 M reads/minute). === Summary === Total read pairs processed: 84,819 Read 1 with adapter: 84,813 (100.0%) Read 2 with adapter: 84,794 (100.0%) == Read fate breakdown == Pairs that were too short: 12 (0.0%) Pairs discarded as untrimmed: 31 (0.0%) Pairs written (passing filters): 84,776 (99.9%) Total basepairs processed: 36,091,419 bp Read 1: 19,722,926 bp Read 2: 16,368,493 bp Total written (filtered): 5,497,908 bp (15.2%) Read 1: 2,923,389 bp Read 2: 2,574,519 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 82576 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1325.3 0 1 1 4 4 331.3 1 2 2 5 2 82.8 1 2 8 2 1.3 2 2 9 3 0.3 2 1 2 10 1 0.1 3 1 12 1 0.0 3 0 1 13 1 0.0 3 1 16 3 0.0 4 2 1 17 1 0.0 5 0 0 1 18 2 0.0 5 1 0 1 19 3 0.0 5 1 2 20 90 0.0 6 86 4 21 66 0.0 6 66 22 45 0.0 6 43 2 23 40 0.0 6 38 2 24 23 0.0 6 21 1 0 0 1 25 31 0.0 6 30 1 26 19 0.0 6 19 27 28 0.0 6 26 1 0 1 28 9 0.0 6 9 29 17 0.0 6 17 30 9 0.0 6 9 31 2 0.0 6 2 32 6 0.0 6 4 2 33 11 0.0 6 11 34 11 0.0 6 10 1 35 8 0.0 6 8 36 7 0.0 6 7 37 9 0.0 6 8 1 38 5 0.0 6 5 39 6 0.0 6 5 1 40 4 0.0 6 4 41 9 0.0 6 8 1 42 3 0.0 6 3 43 7 0.0 6 7 44 7 0.0 6 6 0 0 0 0 1 45 8 0.0 6 8 46 3 0.0 6 3 47 2 0.0 6 2 48 11 0.0 6 10 1 49 9 0.0 6 9 50 9 0.0 6 9 51 8 0.0 6 8 52 7 0.0 6 7 53 11 0.0 6 10 1 54 3 0.0 6 3 55 6 0.0 6 6 56 6 0.0 6 6 57 7 0.0 6 7 58 2 0.0 6 2 59 5 0.0 6 4 1 60 10 0.0 6 10 61 6 0.0 6 6 62 5 0.0 6 5 63 12 0.0 6 12 64 4 0.0 6 4 65 9 0.0 6 9 66 8 0.0 6 8 67 13 0.0 6 13 68 5 0.0 6 3 1 1 69 7 0.0 6 7 70 11 0.0 6 10 0 0 0 1 71 6 0.0 6 6 72 10 0.0 6 10 73 17 0.0 6 16 1 74 10 0.0 6 10 75 17 0.0 6 17 76 6 0.0 6 6 77 7 0.0 6 7 78 14 0.0 6 13 1 79 8 0.0 6 8 80 8 0.0 6 8 81 11 0.0 6 11 82 21 0.0 6 21 83 13 0.0 6 13 84 9 0.0 6 9 85 4 0.0 6 3 1 86 22 0.0 6 20 2 87 5 0.0 6 5 88 16 0.0 6 16 89 10 0.0 6 9 1 90 4 0.0 6 4 91 7 0.0 6 7 92 11 0.0 6 10 1 93 4 0.0 6 4 94 10 0.0 6 10 95 6 0.0 6 6 96 9 0.0 6 8 1 97 15 0.0 6 14 1 98 10 0.0 6 10 99 15 0.0 6 14 1 100 11 0.0 6 10 1 101 17 0.0 6 16 1 102 20 0.0 6 20 103 14 0.0 6 14 104 22 0.0 6 22 105 18 0.0 6 18 106 21 0.0 6 21 107 21 0.0 6 19 2 108 35 0.0 6 34 0 0 1 109 23 0.0 6 23 110 27 0.0 6 27 111 32 0.0 6 32 112 41 0.0 6 38 1 0 1 1 113 30 0.0 6 29 1 114 26 0.0 6 26 115 31 0.0 6 30 0 1 116 32 0.0 6 31 1 117 34 0.0 6 33 1 118 26 0.0 6 24 1 0 0 0 1 119 26 0.0 6 26 120 23 0.0 6 23 121 31 0.0 6 30 1 122 29 0.0 6 29 123 33 0.0 6 33 124 45 0.0 6 45 125 33 0.0 6 33 126 25 0.0 6 25 127 49 0.0 6 49 128 52 0.0 6 52 129 35 0.0 6 35 130 32 0.0 6 31 1 131 43 0.0 6 43 132 52 0.0 6 52 133 58 0.0 6 56 1 0 0 0 1 134 69 0.0 6 69 135 65 0.0 6 65 136 55 0.0 6 55 137 61 0.0 6 60 1 138 77 0.0 6 76 0 0 0 0 1 139 108 0.0 6 108 140 365 0.0 6 365 141 463 0.0 6 463 142 537 0.0 6 537 143 673 0.0 6 673 144 438 0.0 6 438 145 872 0.0 6 871 1 146 1242 0.0 6 1242 147 822 0.0 6 821 1 148 852 0.0 6 846 3 1 1 0 1 149 927 0.0 6 927 150 1003 0.0 6 1001 1 0 0 0 1 151 701 0.0 6 700 1 152 595 0.0 6 593 2 153 623 0.0 6 623 154 679 0.0 6 679 155 425 0.0 6 425 156 584 0.0 6 583 1 157 850 0.0 6 848 0 1 0 0 0 1 158 776 0.0 6 775 1 159 729 0.0 6 725 4 160 717 0.0 6 714 3 161 1011 0.0 6 1009 2 162 776 0.0 6 772 3 1 163 660 0.0 6 658 2 164 724 0.0 6 722 2 165 889 0.0 6 887 1 0 1 166 600 0.0 6 597 3 167 756 0.0 6 756 168 431 0.0 6 431 169 546 0.0 6 544 2 170 638 0.0 6 636 2 171 781 0.0 6 779 2 172 578 0.0 6 578 173 472 0.0 6 469 3 174 607 0.0 6 604 3 175 685 0.0 6 680 5 176 448 0.0 6 448 177 889 0.0 6 886 3 178 873 0.0 6 870 3 179 866 0.0 6 866 180 542 0.0 6 539 2 0 0 0 0 1 181 881 0.0 6 877 4 182 835 0.0 6 831 4 183 695 0.0 6 692 3 184 542 0.0 6 541 1 185 671 0.0 6 669 2 186 653 0.0 6 650 1 1 1 187 452 0.0 6 451 1 188 461 0.0 6 456 4 0 0 0 1 189 573 0.0 6 571 2 190 661 0.0 6 657 4 191 715 0.0 6 713 2 192 484 0.0 6 482 1 0 0 0 0 1 193 672 0.0 6 665 7 194 665 0.0 6 659 6 195 561 0.0 6 556 4 0 0 0 0 1 196 789 0.0 6 786 3 197 895 0.0 6 891 4 198 581 0.0 6 576 4 1 199 533 0.0 6 530 3 200 617 0.0 6 613 3 0 1 201 598 0.0 6 592 4 1 0 0 0 1 202 879 0.0 6 876 3 203 607 0.0 6 605 2 204 701 0.0 6 696 5 205 932 0.0 6 925 6 0 1 206 646 0.0 6 640 6 207 667 0.0 6 663 4 208 689 0.0 6 684 4 0 0 0 0 1 209 671 0.0 6 669 2 210 645 0.0 6 642 3 211 612 0.0 6 609 2 1 212 663 0.0 6 658 5 213 866 0.0 6 863 3 214 1048 0.0 6 1044 4 215 620 0.0 6 616 3 1 216 634 0.0 6 627 6 0 0 0 1 217 657 0.0 6 652 5 218 503 0.0 6 500 3 219 462 0.0 6 462 220 434 0.0 6 434 221 399 0.0 6 397 2 222 510 0.0 6 506 4 223 576 0.0 6 571 4 1 224 534 0.0 6 531 3 225 501 0.0 6 499 2 226 542 0.0 6 540 2 227 495 0.0 6 492 3 228 372 0.0 6 367 5 229 347 0.0 6 343 4 230 364 0.0 6 362 2 231 387 0.0 6 385 1 0 0 0 0 1 232 281 0.0 6 278 2 0 0 1 233 316 0.0 6 313 3 234 286 0.0 6 284 2 235 257 0.0 6 255 1 1 236 252 0.0 6 249 3 237 341 0.0 6 335 5 1 238 260 0.0 6 256 3 0 0 0 1 239 260 0.0 6 257 3 240 327 0.0 6 324 3 241 385 0.0 6 382 3 242 287 0.0 6 278 9 243 389 0.0 6 385 4 244 288 0.0 6 284 4 245 325 0.0 6 321 4 246 187 0.0 6 183 4 247 255 0.0 6 254 1 248 164 0.0 6 161 3 249 293 0.0 6 286 7 250 197 0.0 6 194 3 251 204 0.0 6 201 2 0 1 252 172 0.0 6 166 6 253 177 0.0 6 173 4 254 265 0.0 6 259 5 1 255 171 0.0 6 168 3 256 209 0.0 6 205 4 257 181 0.0 6 171 10 258 263 0.0 6 256 6 0 1 259 226 0.0 6 213 12 0 0 0 1 260 201 0.0 6 196 5 261 145 0.0 6 139 5 0 1 262 159 0.0 6 155 3 1 263 239 0.0 6 233 6 264 176 0.0 6 171 5 265 181 0.0 6 178 1 1 0 0 0 1 266 128 0.0 6 125 3 267 160 0.0 6 158 2 268 223 0.0 6 215 8 269 172 0.0 6 169 2 1 270 107 0.0 6 106 1 271 150 0.0 6 149 1 272 529 0.0 6 516 13 273 347 0.0 6 336 11 274 612 0.0 6 598 14 275 251 0.0 6 242 8 0 0 1 276 650 0.0 6 629 21 277 3891 0.0 6 3795 93 2 0 0 1 278 4326 0.0 6 4193 124 7 2 279 84 0.0 6 81 3 280 10 0.0 6 10 281 3 0.0 6 1 1 0 1 282 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2237 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 3 0.0 6 3 64 72 0.0 6 72 73 10 0.0 6 10 74 16 0.0 6 16 75 14 0.0 6 14 76 20 0.0 6 20 77 19 0.0 6 19 78 43 0.0 6 43 79 38 0.0 6 38 80 23 0.0 6 23 81 23 0.0 6 23 82 33 0.0 6 33 83 26 0.0 6 26 84 20 0.0 6 20 85 19 0.0 6 19 86 22 0.0 6 22 87 19 0.0 6 19 88 14 0.0 6 14 89 17 0.0 6 17 90 27 0.0 6 27 91 21 0.0 6 21 92 21 0.0 6 20 1 93 25 0.0 6 25 94 31 0.0 6 31 95 22 0.0 6 22 96 30 0.0 6 30 97 30 0.0 6 30 98 34 0.0 6 34 99 22 0.0 6 22 100 22 0.0 6 22 101 16 0.0 6 16 102 17 0.0 6 17 103 24 0.0 6 22 2 104 225 0.0 6 220 5 105 13 0.0 6 13 106 10 0.0 6 10 107 8 0.0 6 8 108 10 0.0 6 10 109 16 0.0 6 16 110 31 0.0 6 31 111 33 0.0 6 32 0 0 0 0 0 1 112 26 0.0 6 26 113 23 0.0 6 23 114 31 0.0 6 31 115 22 0.0 6 21 1 116 17 0.0 6 17 117 18 0.0 6 18 118 19 0.0 6 19 119 13 0.0 6 13 120 12 0.0 6 12 121 14 0.0 6 14 122 14 0.0 6 14 123 17 0.0 6 17 124 12 0.0 6 12 125 7 0.0 6 7 126 14 0.0 6 14 127 10 0.0 6 10 128 6 0.0 6 5 0 0 0 0 0 1 129 19 0.0 6 19 130 8 0.0 6 8 131 6 0.0 6 6 132 8 0.0 6 8 133 15 0.0 6 15 134 18 0.0 6 18 135 18 0.0 6 18 136 9 0.0 6 9 137 17 0.0 6 17 138 14 0.0 6 14 139 8 0.0 6 8 140 10 0.0 6 10 141 13 0.0 6 13 142 10 0.0 6 10 143 5 0.0 6 5 144 7 0.0 6 7 145 6 0.0 6 6 146 20 0.0 6 20 147 27 0.0 6 27 148 15 0.0 6 15 149 16 0.0 6 16 150 17 0.0 6 17 151 10 0.0 6 10 152 13 0.0 6 13 153 7 0.0 6 7 154 2 0.0 6 2 155 10 0.0 6 10 156 12 0.0 6 12 157 5 0.0 6 5 158 11 0.0 6 11 159 19 0.0 6 19 160 26 0.0 6 26 161 9 0.0 6 9 162 9 0.0 6 9 163 7 0.0 6 7 164 7 0.0 6 7 165 5 0.0 6 5 166 10 0.0 6 10 167 8 0.0 6 8 168 6 0.0 6 6 169 6 0.0 6 6 170 5 0.0 6 5 171 16 0.0 6 16 172 5 0.0 6 5 173 4 0.0 6 4 174 6 0.0 6 6 175 5 0.0 6 5 176 11 0.0 6 11 177 13 0.0 6 13 178 14 0.0 6 13 1 179 10 0.0 6 10 180 6 0.0 6 6 181 12 0.0 6 12 182 7 0.0 6 7 183 1 0.0 6 1 184 3 0.0 6 3 185 11 0.0 6 11 186 12 0.0 6 12 187 6 0.0 6 6 188 8 0.0 6 8 189 13 0.0 6 13 190 2 0.0 6 2 191 7 0.0 6 7 192 8 0.0 6 8 193 10 0.0 6 10 194 5 0.0 6 5 195 6 0.0 6 6 196 9 0.0 6 9 197 3 0.0 6 3 198 3 0.0 6 3 199 6 0.0 6 6 200 6 0.0 6 6 201 4 0.0 6 4 202 6 0.0 6 6 203 5 0.0 6 5 204 1 0.0 6 1 205 2 0.0 6 2 206 5 0.0 6 5 207 2 0.0 6 2 208 1 0.0 6 1 209 1 0.0 6 1 210 1 0.0 6 1 211 3 0.0 6 3 212 2 0.0 6 2 213 7 0.0 6 7 214 5 0.0 6 5 215 2 0.0 6 2 216 2 0.0 6 2 219 1 0.0 6 1 222 2 0.0 6 2 223 2 0.0 6 2 224 3 0.0 6 3 225 3 0.0 6 3 227 1 0.0 6 1 228 1 0.0 6 1 230 3 0.0 6 3 233 6 0.0 6 6 234 1 0.0 6 1 236 1 0.0 6 1 237 3 0.0 6 3 238 1 0.0 6 1 239 3 0.0 6 3 242 3 0.0 6 3 243 2 0.0 6 2 246 6 0.0 6 6 247 2 0.0 6 2 248 2 0.0 6 2 249 1 0.0 6 1 250 1 0.0 6 1 251 2 0.0 6 2 252 3 0.0 6 3 254 1 0.0 6 1 255 3 0.0 6 3 256 2 0.0 6 2 257 1 0.0 6 1 259 2 0.0 6 2 260 1 0.0 6 1 261 1 0.0 6 1 263 1 0.0 6 1 264 1 0.0 6 1 267 1 0.0 6 1 273 1 0.0 6 1 280 1 0.0 6 1 288 1 0.0 6 1 289 1 0.0 6 1 300 1 0.0 6 1 308 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 82281 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 2 20.7 1 2 7 2 5.2 2 2 8 1 1.3 2 1 9 2 0.3 2 2 10 1 0.1 3 1 11 2 0.0 3 1 1 12 7 0.0 3 6 1 13 5 0.0 3 4 1 14 17 0.0 4 16 1 15 1 0.0 4 1 16 5 0.0 4 5 17 22 0.0 5 20 2 18 6 0.0 5 3 3 19 50 0.0 5 47 2 0 0 1 20 88 0.0 6 84 3 1 21 49 0.0 6 49 22 30 0.0 6 26 3 1 23 23 0.0 6 20 2 1 24 23 0.0 6 21 2 25 17 0.0 6 13 4 26 23 0.0 6 22 1 27 12 0.0 6 11 1 28 24 0.0 6 22 1 0 1 29 9 0.0 6 9 30 12 0.0 6 11 1 31 12 0.0 6 12 32 15 0.0 6 14 1 33 19 0.0 6 17 2 34 12 0.0 6 10 2 35 13 0.0 6 11 2 36 9 0.0 6 7 1 0 0 0 0 1 37 12 0.0 6 11 1 38 13 0.0 6 12 1 39 13 0.0 6 11 2 40 12 0.0 6 12 41 9 0.0 6 9 42 15 0.0 6 15 43 13 0.0 6 12 1 44 24 0.0 6 23 1 45 16 0.0 6 15 1 46 17 0.0 6 16 1 47 12 0.0 6 11 0 0 0 0 0 1 48 16 0.0 6 14 1 1 49 16 0.0 6 14 2 50 13 0.0 6 11 2 51 12 0.0 6 11 1 52 22 0.0 6 21 0 0 0 1 53 14 0.0 6 12 2 54 24 0.0 6 22 2 55 20 0.0 6 17 2 1 56 31 0.0 6 28 3 57 20 0.0 6 18 2 58 24 0.0 6 21 3 59 15 0.0 6 13 1 1 60 20 0.0 6 19 0 0 1 61 19 0.0 6 18 1 62 17 0.0 6 15 1 0 1 63 22 0.0 6 17 4 0 1 64 17 0.0 6 17 65 26 0.0 6 26 66 25 0.0 6 22 3 67 26 0.0 6 22 3 0 0 1 68 34 0.0 6 32 1 0 1 69 42 0.0 6 39 3 70 143 0.0 6 140 3 71 55 0.0 6 48 6 1 72 87 0.0 6 86 1 73 38 0.0 6 33 4 0 0 0 1 74 39 0.0 6 38 1 75 32 0.0 6 32 76 23 0.0 6 20 2 1 77 32 0.0 6 31 0 1 78 35 0.0 6 33 1 0 0 1 79 48 0.0 6 48 80 40 0.0 6 38 2 81 51 0.0 6 49 2 82 72 0.0 6 66 5 1 83 42 0.0 6 40 2 84 51 0.0 6 48 2 0 1 85 52 0.0 6 50 2 86 90 0.0 6 85 4 0 0 1 87 52 0.0 6 51 1 88 36 0.0 6 36 89 54 0.0 6 49 5 90 44 0.0 6 42 2 91 48 0.0 6 45 2 1 92 48 0.0 6 46 2 93 70 0.0 6 68 2 94 80 0.0 6 76 4 95 52 0.0 6 52 96 89 0.0 6 85 3 1 97 60 0.0 6 56 3 1 98 78 0.0 6 74 2 0 2 99 81 0.0 6 77 3 1 100 83 0.0 6 81 2 101 67 0.0 6 63 4 102 81 0.0 6 75 5 0 1 103 75 0.0 6 74 1 104 85 0.0 6 84 1 105 84 0.0 6 82 2 106 96 0.0 6 95 1 107 165 0.0 6 158 7 108 93 0.0 6 86 5 1 0 0 0 1 109 89 0.0 6 83 5 1 110 107 0.0 6 104 3 111 106 0.0 6 104 2 112 102 0.0 6 102 113 127 0.0 6 126 1 114 129 0.0 6 127 2 115 166 0.0 6 164 2 116 135 0.0 6 133 0 1 0 1 117 115 0.0 6 114 1 118 124 0.0 6 120 3 0 0 0 1 119 136 0.0 6 131 5 120 199 0.0 6 196 1 0 1 0 0 1 121 170 0.0 6 164 5 0 0 1 122 185 0.0 6 181 4 123 176 0.0 6 173 2 0 0 0 0 1 124 166 0.0 6 162 3 1 125 166 0.0 6 163 1 1 0 0 0 1 126 193 0.0 6 193 127 224 0.0 6 219 5 128 260 0.0 6 257 3 129 233 0.0 6 229 4 130 269 0.0 6 265 4 131 331 0.0 6 326 4 0 0 0 0 1 132 380 0.0 6 378 2 133 329 0.0 6 322 5 0 0 0 0 2 134 375 0.0 6 369 4 0 0 1 1 135 411 0.0 6 407 4 136 407 0.0 6 404 3 137 492 0.0 6 486 3 1 0 0 1 1 138 436 0.0 6 435 1 139 453 0.0 6 450 2 0 0 0 1 140 523 0.0 6 517 5 0 1 141 705 0.0 6 697 6 0 1 0 0 1 142 832 0.0 6 823 8 0 0 0 1 143 923 0.0 6 917 6 144 1102 0.0 6 1089 11 2 145 1072 0.0 6 1061 10 0 1 146 1224 0.0 6 1215 7 2 147 1549 0.0 6 1542 5 0 0 1 1 148 1207 0.0 6 1198 9 149 1352 0.0 6 1342 7 1 1 0 1 150 1464 0.0 6 1452 11 0 0 0 0 1 151 1531 0.0 6 1518 12 0 0 0 1 152 1453 0.0 6 1441 9 1 0 1 0 1 153 1719 0.0 6 1704 15 154 1397 0.0 6 1385 10 2 155 1598 0.0 6 1583 13 0 1 0 0 1 156 1456 0.0 6 1438 16 1 0 0 1 157 1282 0.0 6 1272 7 2 0 1 158 1554 0.0 6 1542 11 1 159 1263 0.0 6 1252 10 0 0 0 1 160 1390 0.0 6 1374 10 2 2 1 1 161 1396 0.0 6 1391 5 162 1271 0.0 6 1266 4 0 0 0 1 163 1265 0.0 6 1254 10 0 0 0 0 1 164 1133 0.0 6 1127 4 1 0 0 0 1 165 1112 0.0 6 1106 5 0 0 1 166 1170 0.0 6 1159 10 0 0 0 1 167 1034 0.0 6 1028 5 0 1 168 1009 0.0 6 1006 3 169 990 0.0 6 984 5 0 0 0 0 1 170 922 0.0 6 914 7 0 0 0 1 171 1245 0.0 6 1238 7 172 1020 0.0 6 1012 7 0 0 0 1 173 908 0.0 6 899 8 0 0 0 1 174 1075 0.0 6 1065 10 175 1137 0.0 6 1122 11 0 2 0 2 176 1064 0.0 6 1058 6 177 1052 0.0 6 1042 9 0 1 178 1073 0.0 6 1056 16 0 0 0 0 1 179 1105 0.0 6 1100 5 180 847 0.0 6 841 5 0 1 181 993 0.0 6 986 7 182 711 0.0 6 698 11 0 1 0 0 1 183 773 0.0 6 767 6 184 569 0.0 6 565 3 0 0 1 185 489 0.0 6 484 5 186 882 0.0 6 873 8 0 1 187 1115 0.0 6 1093 19 1 2 188 1090 0.0 6 1069 20 0 0 0 0 1 189 743 0.0 6 734 6 0 1 2 190 950 0.0 6 931 19 191 842 0.0 6 830 11 1 192 690 0.0 6 676 14 193 597 0.0 6 592 4 0 0 1 194 574 0.0 6 565 9 195 565 0.0 6 549 16 196 874 0.0 6 856 16 0 1 1 197 464 0.0 6 452 10 2 198 531 0.0 6 511 18 2 199 1104 0.0 6 1078 25 0 1 200 462 0.0 6 454 8 201 643 0.0 6 634 9 202 487 0.0 6 472 14 0 0 1 203 367 0.0 6 359 8 204 381 0.0 6 374 7 205 246 0.0 6 240 4 0 2 206 242 0.0 6 238 4 207 174 0.0 6 170 4 208 237 0.0 6 231 6 209 248 0.0 6 244 4 210 199 0.0 6 197 2 211 272 0.0 6 262 10 212 258 0.0 6 255 3 213 123 0.0 6 116 7 214 171 0.0 6 168 3 215 183 0.0 6 180 3 216 213 0.0 6 207 6 217 382 0.0 6 377 4 0 0 1 218 201 0.0 6 198 3 219 216 0.0 6 210 6 220 138 0.0 6 136 2 221 191 0.0 6 187 4 222 88 0.0 6 86 2 223 148 0.0 6 146 2 224 167 0.0 6 164 3 225 206 0.0 6 202 4 226 226 0.0 6 220 6 227 201 0.0 6 196 5 228 86 0.0 6 85 0 0 0 1 229 141 0.0 6 140 1 230 61 0.0 6 58 3 231 62 0.0 6 61 1 232 116 0.0 6 108 7 0 0 1 233 125 0.0 6 122 3 234 69 0.0 6 67 2 235 61 0.0 6 59 2 236 18 0.0 6 18 237 33 0.0 6 30 2 0 0 1 238 20 0.0 6 20 239 7 0.0 6 5 0 0 1 1 240 21 0.0 6 21 241 38 0.0 6 37 1 242 66 0.0 6 64 2 243 55 0.0 6 53 2 244 96 0.0 6 93 3 245 30 0.0 6 29 1 246 19 0.0 6 18 1 247 14 0.0 6 14 248 13 0.0 6 13 249 12 0.0 6 12 250 11 0.0 6 10 1 251 35 0.0 6 34 1 252 13 0.0 6 12 1 253 11 0.0 6 10 1 254 24 0.0 6 24 255 2 0.0 6 2 256 1 0.0 6 1 257 1 0.0 6 1 258 2 0.0 6 2 280 4 0.0 6 4 281 20 0.0 6 20 282 58 0.0 6 56 1 0 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2513 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 1 0.0 6 0 1 52 1 0.0 6 1 57 1 0.0 6 1 60 1 0.0 6 1 67 1 0.0 6 1 68 1 0.0 6 1 69 14 0.0 6 14 70 16 0.0 6 16 71 20 0.0 6 20 72 55 0.0 6 55 73 16 0.0 6 16 74 32 0.0 6 32 75 38 0.0 6 38 76 24 0.0 6 24 77 42 0.0 6 42 78 30 0.0 6 29 0 0 1 79 34 0.0 6 34 80 23 0.0 6 23 81 19 0.0 6 19 82 20 0.0 6 20 83 21 0.0 6 21 84 10 0.0 6 10 85 26 0.0 6 26 86 34 0.0 6 34 87 31 0.0 6 31 88 16 0.0 6 16 89 32 0.0 6 32 90 49 0.0 6 49 91 193 0.0 6 192 1 92 21 0.0 6 20 1 93 30 0.0 6 29 0 0 1 94 31 0.0 6 28 1 0 1 1 95 27 0.0 6 25 0 0 0 2 96 20 0.0 6 20 97 15 0.0 6 15 98 18 0.0 6 18 99 17 0.0 6 17 100 34 0.0 6 34 101 15 0.0 6 15 102 10 0.0 6 10 103 20 0.0 6 19 1 104 268 0.0 6 265 3 105 20 0.0 6 20 106 42 0.0 6 42 107 34 0.0 6 34 108 26 0.0 6 25 0 0 0 1 109 24 0.0 6 23 1 110 23 0.0 6 23 111 24 0.0 6 24 112 18 0.0 6 17 0 0 0 1 113 14 0.0 6 14 114 21 0.0 6 21 115 28 0.0 6 28 116 4 0.0 6 4 117 13 0.0 6 13 118 21 0.0 6 21 119 22 0.0 6 22 120 19 0.0 6 19 121 10 0.0 6 10 122 14 0.0 6 14 123 6 0.0 6 6 124 7 0.0 6 7 125 19 0.0 6 19 126 42 0.0 6 42 127 10 0.0 6 9 0 0 1 128 15 0.0 6 15 129 12 0.0 6 12 130 16 0.0 6 16 131 25 0.0 6 24 0 0 1 132 14 0.0 6 11 1 0 0 2 133 22 0.0 6 22 134 17 0.0 6 17 135 8 0.0 6 8 136 11 0.0 6 11 137 15 0.0 6 15 138 15 0.0 6 15 139 10 0.0 6 10 140 6 0.0 6 6 141 15 0.0 6 15 142 24 0.0 6 24 143 31 0.0 6 31 144 17 0.0 6 17 145 16 0.0 6 16 146 20 0.0 6 20 147 11 0.0 6 11 148 11 0.0 6 11 149 8 0.0 6 8 150 3 0.0 6 3 151 16 0.0 6 16 152 18 0.0 6 18 153 5 0.0 6 5 154 12 0.0 6 12 155 22 0.0 6 22 156 31 0.0 6 30 1 157 11 0.0 6 11 158 9 0.0 6 9 159 7 0.0 6 7 160 15 0.0 6 15 161 1 0.0 6 1 162 11 0.0 6 11 163 5 0.0 6 5 164 7 0.0 6 7 165 5 0.0 6 5 166 2 0.0 6 2 167 3 0.0 6 3 168 8 0.0 6 8 169 5 0.0 6 5 170 7 0.0 6 7 171 7 0.0 6 7 172 11 0.0 6 11 173 19 0.0 6 19 174 11 0.0 6 11 175 16 0.0 6 16 176 13 0.0 6 13 177 5 0.0 6 4 0 0 0 1 178 8 0.0 6 8 180 5 0.0 6 5 181 9 0.0 6 9 182 4 0.0 6 3 0 0 0 0 1 183 3 0.0 6 3 184 3 0.0 6 3 185 10 0.0 6 10 186 2 0.0 6 2 187 5 0.0 6 5 188 6 0.0 6 6 189 9 0.0 6 9 190 4 0.0 6 4 191 2 0.0 6 2 192 4 0.0 6 4 193 1 0.0 6 1 194 2 0.0 6 2 195 1 0.0 6 1 196 1 0.0 6 1 197 2 0.0 6 2 199 2 0.0 6 2 200 2 0.0 6 2 202 4 0.0 6 4 207 1 0.0 6 1 209 4 0.0 6 4 210 1 0.0 6 1 212 1 0.0 6 1 214 1 0.0 6 1 221 1 0.0 6 1 236 1 0.0 6 1 237 1 0.0 6 1 239 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_18_33_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_18_260_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_18_33_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_18_260_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.98 s (30 µs/read; 2.01 M reads/minute). === Summary === Total read pairs processed: 66,568 Read 1 with adapter: 66,561 (100.0%) Read 2 with adapter: 66,535 (100.0%) == Read fate breakdown == Pairs that were too short: 13 (0.0%) Pairs discarded as untrimmed: 40 (0.1%) Pairs written (passing filters): 66,515 (99.9%) Total basepairs processed: 28,014,115 bp Read 1: 14,846,357 bp Read 2: 13,167,758 bp Total written (filtered): 4,582,578 bp (16.4%) Read 1: 2,344,216 bp Read 2: 2,238,362 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 64045 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 1040.1 0 2 1 4 2 260.0 1 0 2 5 1 65.0 1 1 6 1 16.3 1 0 0 1 7 3 4.1 2 2 0 1 11 1 0.0 3 1 14 3 0.0 4 2 1 16 3 0.0 4 2 1 17 4 0.0 5 3 0 0 0 1 18 2 0.0 5 2 19 4 0.0 5 3 1 20 86 0.0 6 84 2 21 37 0.0 6 36 1 22 39 0.0 6 36 3 23 62 0.0 6 58 4 24 28 0.0 6 25 3 25 21 0.0 6 21 26 12 0.0 6 11 1 27 22 0.0 6 20 2 28 12 0.0 6 12 29 5 0.0 6 4 0 0 0 0 0 1 30 1 0.0 6 1 31 5 0.0 6 5 32 4 0.0 6 3 1 33 3 0.0 6 2 0 1 34 14 0.0 6 14 35 6 0.0 6 6 36 5 0.0 6 5 37 12 0.0 6 10 2 38 9 0.0 6 9 39 13 0.0 6 13 40 2 0.0 6 2 41 6 0.0 6 5 1 42 6 0.0 6 4 2 43 1 0.0 6 1 44 4 0.0 6 4 45 7 0.0 6 7 46 2 0.0 6 2 47 2 0.0 6 2 48 6 0.0 6 6 49 6 0.0 6 5 1 50 3 0.0 6 3 51 3 0.0 6 3 52 4 0.0 6 4 53 6 0.0 6 6 54 9 0.0 6 9 55 3 0.0 6 3 56 3 0.0 6 3 57 7 0.0 6 7 58 6 0.0 6 6 59 3 0.0 6 3 60 3 0.0 6 2 1 61 7 0.0 6 7 62 1 0.0 6 1 63 4 0.0 6 4 64 5 0.0 6 5 65 4 0.0 6 4 66 8 0.0 6 8 67 6 0.0 6 5 1 68 6 0.0 6 6 69 10 0.0 6 10 70 3 0.0 6 3 71 6 0.0 6 6 72 13 0.0 6 12 1 73 14 0.0 6 14 74 5 0.0 6 5 75 2 0.0 6 1 1 76 4 0.0 6 4 77 7 0.0 6 6 1 78 3 0.0 6 3 79 9 0.0 6 7 2 80 14 0.0 6 14 81 6 0.0 6 4 2 82 7 0.0 6 7 83 13 0.0 6 13 84 18 0.0 6 17 0 1 85 5 0.0 6 5 86 13 0.0 6 13 87 8 0.0 6 8 88 14 0.0 6 13 1 89 5 0.0 6 4 1 90 5 0.0 6 5 91 5 0.0 6 5 92 6 0.0 6 4 2 93 8 0.0 6 8 94 7 0.0 6 7 95 5 0.0 6 5 96 7 0.0 6 7 97 6 0.0 6 6 98 5 0.0 6 5 99 12 0.0 6 12 100 14 0.0 6 12 2 101 16 0.0 6 15 0 1 102 20 0.0 6 20 103 24 0.0 6 24 104 25 0.0 6 25 105 21 0.0 6 21 106 12 0.0 6 12 107 17 0.0 6 17 108 14 0.0 6 13 1 109 25 0.0 6 25 110 45 0.0 6 45 111 41 0.0 6 40 1 112 38 0.0 6 38 113 15 0.0 6 15 114 12 0.0 6 12 115 14 0.0 6 14 116 11 0.0 6 11 117 13 0.0 6 12 1 118 21 0.0 6 21 119 22 0.0 6 22 120 12 0.0 6 12 121 35 0.0 6 35 122 38 0.0 6 38 123 30 0.0 6 30 124 14 0.0 6 14 125 18 0.0 6 18 126 28 0.0 6 28 127 26 0.0 6 25 0 0 1 128 20 0.0 6 20 129 29 0.0 6 29 130 23 0.0 6 23 131 32 0.0 6 31 1 132 29 0.0 6 29 133 26 0.0 6 26 134 41 0.0 6 41 135 45 0.0 6 45 136 53 0.0 6 53 137 41 0.0 6 41 138 52 0.0 6 52 139 54 0.0 6 54 140 90 0.0 6 88 2 141 139 0.0 6 139 142 350 0.0 6 350 143 2249 0.0 6 2245 4 144 2598 0.0 6 2597 1 145 2258 0.0 6 2256 2 146 292 0.0 6 291 1 147 92 0.0 6 91 1 148 123 0.0 6 123 149 489 0.0 6 487 2 150 179 0.0 6 179 151 84 0.0 6 83 1 152 97 0.0 6 97 153 139 0.0 6 139 154 297 0.0 6 294 3 155 2011 0.0 6 2009 1 0 1 156 1933 0.0 6 1928 4 1 157 344 0.0 6 343 1 158 110 0.0 6 110 159 53 0.0 6 51 1 0 1 160 48 0.0 6 48 161 78 0.0 6 78 162 63 0.0 6 62 1 163 71 0.0 6 71 164 115 0.0 6 115 165 404 0.0 6 402 2 166 95 0.0 6 94 1 167 71 0.0 6 70 0 0 0 0 0 1 168 98 0.0 6 97 1 169 84 0.0 6 84 170 154 0.0 6 152 2 171 224 0.0 6 224 172 101 0.0 6 101 173 57 0.0 6 57 174 103 0.0 6 103 175 124 0.0 6 123 1 176 150 0.0 6 149 1 177 461 0.0 6 459 2 178 371 0.0 6 371 179 111 0.0 6 111 180 69 0.0 6 69 181 161 0.0 6 161 182 165 0.0 6 165 183 139 0.0 6 137 2 184 154 0.0 6 154 185 212 0.0 6 211 1 186 196 0.0 6 196 187 202 0.0 6 202 188 303 0.0 6 302 0 1 189 320 0.0 6 320 190 699 0.0 6 697 2 191 3937 0.0 6 3932 5 192 654 0.0 6 654 193 289 0.0 6 287 2 194 480 0.0 6 477 3 195 533 0.0 6 530 2 0 1 196 968 0.0 6 963 5 197 3781 0.0 6 3776 5 198 523 0.0 6 522 1 199 498 0.0 6 497 1 200 419 0.0 6 416 3 201 480 0.0 6 479 1 202 699 0.0 6 697 2 203 1206 0.0 6 1203 3 204 5277 0.0 6 5272 5 205 1185 0.0 6 1183 1 0 0 0 0 1 206 947 0.0 6 942 4 1 207 1074 0.0 6 1073 1 208 1027 0.0 6 1024 1 0 1 1 209 1219 0.0 6 1217 1 0 0 0 1 210 3364 0.0 6 3354 7 0 0 1 0 2 211 4516 0.0 6 4506 9 0 0 1 212 626 0.0 6 624 2 213 570 0.0 6 569 1 214 738 0.0 6 738 215 448 0.0 6 447 1 216 307 0.0 6 303 3 0 0 0 1 217 213 0.0 6 211 2 218 386 0.0 6 385 1 219 526 0.0 6 525 1 220 1144 0.0 6 1141 2 0 1 221 50 0.0 6 49 1 222 63 0.0 6 62 0 0 1 223 95 0.0 6 92 3 224 53 0.0 6 51 2 225 49 0.0 6 48 1 226 47 0.0 6 47 227 58 0.0 6 58 228 44 0.0 6 42 2 229 62 0.0 6 61 1 230 63 0.0 6 63 231 92 0.0 6 91 1 232 117 0.0 6 115 2 233 95 0.0 6 95 234 66 0.0 6 65 1 235 101 0.0 6 99 1 0 0 1 236 39 0.0 6 38 1 237 118 0.0 6 117 1 238 52 0.0 6 50 2 239 97 0.0 6 96 0 1 240 149 0.0 6 148 1 241 95 0.0 6 93 2 242 73 0.0 6 73 243 80 0.0 6 80 244 77 0.0 6 77 245 77 0.0 6 77 246 50 0.0 6 50 247 66 0.0 6 66 248 74 0.0 6 74 249 68 0.0 6 67 1 250 95 0.0 6 93 2 251 107 0.0 6 106 1 252 95 0.0 6 93 2 253 131 0.0 6 129 2 254 40 0.0 6 40 255 56 0.0 6 54 2 256 24 0.0 6 23 1 257 38 0.0 6 37 1 258 38 0.0 6 37 1 259 26 0.0 6 26 260 38 0.0 6 37 1 261 29 0.0 6 29 262 44 0.0 6 44 263 20 0.0 6 19 1 264 44 0.0 6 43 1 265 26 0.0 6 26 266 50 0.0 6 50 267 55 0.0 6 55 268 40 0.0 6 40 269 51 0.0 6 51 270 63 0.0 6 62 1 271 36 0.0 6 36 272 62 0.0 6 62 273 185 0.0 6 182 3 274 20 0.0 6 20 275 24 0.0 6 24 276 80 0.0 6 80 277 262 0.0 6 260 2 278 655 0.0 6 655 279 9 0.0 6 9 280 1 0.0 6 0 0 0 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2516 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 3 0.0 6 3 72 37 0.0 6 37 73 109 0.0 6 108 1 74 125 0.0 6 125 75 85 0.0 6 85 76 4 0.0 6 4 77 1 0.0 6 1 78 7 0.0 6 7 79 17 0.0 6 16 0 0 0 0 0 1 80 4 0.0 6 4 82 6 0.0 6 6 83 3 0.0 6 3 84 29 0.0 6 28 1 85 95 0.0 6 95 86 74 0.0 6 74 87 3 0.0 6 3 88 1 0.0 6 1 89 1 0.0 6 1 91 1 0.0 6 1 94 2 0.0 6 2 95 10 0.0 6 10 106 4 0.0 6 4 107 10 0.0 6 10 113 1 0.0 6 1 114 4 0.0 6 4 117 4 0.0 6 4 118 5 0.0 6 5 119 4 0.0 6 4 120 66 0.0 6 65 1 121 170 0.0 6 169 1 122 8 0.0 6 8 123 1 0.0 6 1 125 3 0.0 6 3 126 58 0.0 6 58 127 134 0.0 6 134 128 11 0.0 6 11 129 3 0.0 6 3 130 1 0.0 6 1 132 4 0.0 6 3 1 133 97 0.0 6 97 134 276 0.0 6 276 135 10 0.0 6 10 137 1 0.0 6 1 138 5 0.0 6 5 139 61 0.0 6 60 1 140 211 0.0 6 211 141 308 0.0 6 308 142 12 0.0 6 12 143 4 0.0 6 4 144 5 0.0 6 5 145 15 0.0 6 15 147 1 0.0 6 0 0 0 0 0 0 1 150 1 0.0 6 1 161 1 0.0 6 1 162 2 0.0 6 2 163 6 0.0 6 6 169 1 0.0 6 1 178 1 0.0 6 1 179 2 0.0 6 2 181 3 0.0 6 3 182 69 0.0 6 69 183 184 0.0 6 184 184 5 0.0 6 5 205 3 0.0 6 3 206 3 0.0 6 3 207 6 0.0 6 6 208 22 0.0 6 22 209 2 0.0 6 2 212 1 0.0 6 1 213 8 0.0 6 8 214 27 0.0 6 27 268 1 0.0 6 1 270 13 0.0 6 13 271 38 0.0 6 38 272 2 0.0 6 2 274 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 63951 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 4.1 2 1 9 1 0.3 2 1 10 2 0.1 3 0 2 11 1 0.0 3 1 12 1 0.0 3 1 13 6 0.0 3 6 14 2 0.0 4 2 16 9 0.0 4 8 1 17 14 0.0 5 13 1 18 6 0.0 5 5 1 19 6 0.0 5 4 2 20 60 0.0 6 58 2 21 31 0.0 6 30 1 22 19 0.0 6 18 1 23 11 0.0 6 11 24 26 0.0 6 24 1 1 25 15 0.0 6 14 1 26 17 0.0 6 17 27 15 0.0 6 15 28 17 0.0 6 16 1 29 6 0.0 6 6 30 9 0.0 6 9 31 4 0.0 6 4 32 3 0.0 6 3 33 6 0.0 6 6 34 7 0.0 6 7 35 6 0.0 6 6 36 9 0.0 6 8 1 37 6 0.0 6 6 38 6 0.0 6 4 2 39 10 0.0 6 10 40 8 0.0 6 6 2 41 11 0.0 6 9 2 42 6 0.0 6 6 43 11 0.0 6 10 0 1 44 7 0.0 6 6 1 45 8 0.0 6 8 46 9 0.0 6 8 1 47 5 0.0 6 5 48 11 0.0 6 11 49 10 0.0 6 9 0 1 50 6 0.0 6 6 51 6 0.0 6 4 2 52 13 0.0 6 12 1 53 12 0.0 6 12 54 5 0.0 6 5 55 15 0.0 6 13 2 56 4 0.0 6 4 57 10 0.0 6 10 58 11 0.0 6 8 3 59 15 0.0 6 13 1 0 0 0 1 60 15 0.0 6 14 0 0 0 1 61 4 0.0 6 4 62 12 0.0 6 11 1 63 18 0.0 6 17 1 64 12 0.0 6 8 3 1 65 13 0.0 6 13 66 26 0.0 6 24 1 1 67 100 0.0 6 97 1 1 1 68 29 0.0 6 26 0 2 1 69 50 0.0 6 48 2 70 10 0.0 6 8 2 71 36 0.0 6 32 3 1 72 19 0.0 6 18 1 73 14 0.0 6 13 1 74 19 0.0 6 19 75 17 0.0 6 16 1 76 21 0.0 6 20 0 0 0 1 77 25 0.0 6 23 1 0 1 78 26 0.0 6 24 2 79 19 0.0 6 17 0 0 0 2 80 27 0.0 6 27 81 20 0.0 6 16 4 82 18 0.0 6 18 83 40 0.0 6 39 1 84 13 0.0 6 12 1 85 18 0.0 6 17 0 0 0 0 1 86 25 0.0 6 24 0 0 0 0 0 1 87 28 0.0 6 28 88 35 0.0 6 34 1 89 20 0.0 6 20 90 35 0.0 6 35 91 33 0.0 6 33 92 23 0.0 6 20 3 93 37 0.0 6 36 1 94 39 0.0 6 36 3 95 31 0.0 6 31 96 45 0.0 6 42 2 0 0 0 1 97 30 0.0 6 29 1 98 40 0.0 6 38 2 99 31 0.0 6 30 1 100 37 0.0 6 37 101 35 0.0 6 34 1 102 31 0.0 6 28 3 103 38 0.0 6 37 1 104 69 0.0 6 69 105 35 0.0 6 34 1 106 43 0.0 6 41 2 107 45 0.0 6 44 0 0 0 1 108 63 0.0 6 62 0 0 0 0 0 1 109 54 0.0 6 53 1 110 68 0.0 6 68 111 81 0.0 6 80 1 112 82 0.0 6 81 0 1 113 63 0.0 6 63 114 59 0.0 6 58 0 0 0 0 1 115 60 0.0 6 60 116 82 0.0 6 78 3 1 117 87 0.0 6 86 1 118 74 0.0 6 74 119 81 0.0 6 80 1 120 58 0.0 6 58 121 95 0.0 6 94 1 122 96 0.0 6 95 0 0 0 0 1 123 88 0.0 6 87 1 124 134 0.0 6 133 0 1 125 135 0.0 6 135 126 118 0.0 6 118 127 132 0.0 6 131 0 0 0 1 128 139 0.0 6 136 3 129 121 0.0 6 119 1 0 0 0 1 130 165 0.0 6 165 131 181 0.0 6 180 1 132 176 0.0 6 175 1 133 241 0.0 6 238 3 134 267 0.0 6 267 135 307 0.0 6 307 136 305 0.0 6 303 2 137 340 0.0 6 338 1 0 0 1 138 346 0.0 6 343 3 139 428 0.0 6 423 4 0 0 1 140 695 0.0 6 692 2 0 0 0 1 141 2125 0.0 6 2119 6 142 2482 0.0 6 2478 2 1 0 0 1 143 1667 0.0 6 1660 5 1 0 1 144 477 0.0 6 474 3 145 318 0.0 6 314 3 0 0 0 1 146 390 0.0 6 386 4 147 610 0.0 6 605 2 0 0 1 1 1 148 683 0.0 6 672 9 1 0 1 149 565 0.0 6 557 6 0 0 0 2 150 967 0.0 6 960 6 1 151 894 0.0 6 889 5 152 947 0.0 6 939 6 2 153 2075 0.0 6 2071 1 3 154 1390 0.0 6 1387 1 1 0 1 155 560 0.0 6 558 1 1 156 349 0.0 6 348 1 157 612 0.0 6 612 158 693 0.0 6 691 1 0 0 0 0 1 159 435 0.0 6 432 3 160 431 0.0 6 427 4 161 452 0.0 6 445 5 1 1 162 540 0.0 6 535 5 163 595 0.0 6 591 3 1 164 637 0.0 6 632 5 165 485 0.0 6 481 4 166 555 0.0 6 552 2 0 0 1 167 443 0.0 6 440 2 0 0 0 0 1 168 896 0.0 6 891 3 2 169 1039 0.0 6 1034 4 0 0 0 0 1 170 886 0.0 6 880 6 171 894 0.0 6 884 10 172 1085 0.0 6 1082 1 1 0 0 0 1 173 672 0.0 6 666 6 174 780 0.0 6 778 1 0 0 0 1 175 1041 0.0 6 1038 1 0 2 176 979 0.0 6 977 1 1 177 800 0.0 6 798 2 178 1050 0.0 6 1049 1 179 940 0.0 6 937 3 180 639 0.0 6 637 2 181 546 0.0 6 544 2 182 664 0.0 6 663 0 1 183 1062 0.0 6 1058 2 0 0 0 0 2 184 1346 0.0 6 1345 1 185 1724 0.0 6 1722 2 186 1093 0.0 6 1089 3 0 0 0 0 1 187 1281 0.0 6 1278 2 0 0 1 188 1200 0.0 6 1191 8 1 189 1529 0.0 6 1529 190 943 0.0 6 936 3 0 2 2 191 1135 0.0 6 1129 6 192 1172 0.0 6 1168 3 0 1 193 632 0.0 6 631 1 194 568 0.0 6 567 1 195 736 0.0 6 735 1 196 502 0.0 6 501 0 0 1 197 435 0.0 6 435 198 422 0.0 6 422 199 479 0.0 6 476 3 200 476 0.0 6 472 3 1 201 493 0.0 6 490 3 202 678 0.0 6 678 203 333 0.0 6 331 1 1 204 378 0.0 6 375 3 205 266 0.0 6 265 1 206 404 0.0 6 402 1 1 207 388 0.0 6 387 1 208 550 0.0 6 547 3 209 414 0.0 6 411 2 1 210 31 0.0 6 31 211 62 0.0 6 61 1 212 63 0.0 6 62 1 213 50 0.0 6 50 214 48 0.0 6 48 215 40 0.0 6 37 3 216 13 0.0 6 13 217 30 0.0 6 28 2 218 20 0.0 6 20 219 27 0.0 6 26 1 220 27 0.0 6 26 1 221 44 0.0 6 43 1 222 37 0.0 6 33 4 223 13 0.0 6 13 224 18 0.0 6 16 2 225 17 0.0 6 16 1 226 20 0.0 6 19 1 227 14 0.0 6 14 228 6 0.0 6 6 229 13 0.0 6 13 230 6 0.0 6 6 231 15 0.0 6 15 232 12 0.0 6 12 233 5 0.0 6 5 234 5 0.0 6 5 235 3 0.0 6 3 236 2 0.0 6 2 237 1 0.0 6 1 238 1 0.0 6 1 239 1 0.0 6 1 242 1 0.0 6 1 245 1 0.0 6 1 266 1 0.0 6 1 277 1 0.0 6 1 278 3 0.0 6 3 279 31 0.0 6 31 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2584 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 3 0.0 6 3 71 27 0.0 6 27 72 124 0.0 6 124 73 119 0.0 6 118 1 74 104 0.0 6 103 1 75 3 0.0 6 3 76 2 0.0 6 1 0 0 0 1 77 4 0.0 6 4 78 23 0.0 6 23 79 5 0.0 6 5 81 2 0.0 6 2 82 1 0.0 6 1 83 29 0.0 6 28 1 84 106 0.0 6 106 85 99 0.0 6 99 86 1 0.0 6 1 89 4 0.0 6 3 0 0 0 1 90 3 0.0 6 3 93 5 0.0 6 5 94 24 0.0 6 24 99 1 0.0 6 1 105 4 0.0 6 4 106 12 0.0 6 12 107 4 0.0 6 4 108 1 0.0 6 1 109 1 0.0 6 1 112 1 0.0 6 1 113 2 0.0 6 2 116 2 0.0 6 2 117 3 0.0 6 3 118 4 0.0 6 4 119 48 0.0 6 48 120 175 0.0 6 175 121 8 0.0 6 7 1 123 1 0.0 6 1 124 8 0.0 6 8 125 38 0.0 6 38 126 178 0.0 6 178 127 3 0.0 6 3 128 1 0.0 6 1 129 3 0.0 6 2 0 0 1 130 4 0.0 6 3 0 0 0 1 131 5 0.0 6 5 132 77 0.0 6 76 1 133 302 0.0 6 302 134 10 0.0 6 10 136 4 0.0 6 4 137 9 0.0 6 5 0 0 0 4 138 28 0.0 6 28 139 265 0.0 6 263 2 140 381 0.0 6 380 1 141 9 0.0 6 9 142 1 0.0 6 1 143 8 0.0 6 7 0 0 0 1 144 24 0.0 6 22 0 0 0 2 145 1 0.0 6 1 149 1 0.0 6 1 162 7 0.0 6 7 177 2 0.0 6 2 179 2 0.0 6 2 180 3 0.0 6 3 181 33 0.0 6 33 182 192 0.0 6 192 183 5 0.0 6 5 185 1 0.0 6 1 204 2 0.0 6 2 205 5 0.0 6 5 206 3 0.0 6 3 207 3 0.0 6 3 211 1 0.0 6 1 213 10 0.0 6 10 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_19_34_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_19_261_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_19_34_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_19_261_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.39 s (21 µs/read; 2.84 M reads/minute). === Summary === Total read pairs processed: 65,703 Read 1 with adapter: 65,693 (100.0%) Read 2 with adapter: 65,668 (99.9%) == Read fate breakdown == Pairs that were too short: 24 (0.0%) Pairs discarded as untrimmed: 45 (0.1%) Pairs written (passing filters): 65,634 (99.9%) Total basepairs processed: 27,511,168 bp Read 1: 15,243,477 bp Read 2: 12,267,691 bp Total written (filtered): 4,278,780 bp (15.6%) Read 1: 2,253,265 bp Read 2: 2,025,515 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 63945 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1026.6 0 1 4 3 256.7 1 0 3 5 1 64.2 1 1 6 1 16.0 1 0 0 1 7 2 4.0 2 1 0 1 9 3 0.3 2 3 10 2 0.1 3 2 13 1 0.0 3 1 14 3 0.0 4 2 1 17 3 0.0 5 2 1 19 2 0.0 5 1 1 20 129 0.0 6 126 3 21 62 0.0 6 59 3 22 51 0.0 6 50 1 23 31 0.0 6 31 24 20 0.0 6 20 25 11 0.0 6 11 26 12 0.0 6 11 1 27 11 0.0 6 8 3 28 11 0.0 6 11 29 5 0.0 6 5 30 2 0.0 6 2 31 10 0.0 6 10 32 7 0.0 6 7 33 3 0.0 6 3 34 9 0.0 6 8 1 35 8 0.0 6 8 36 4 0.0 6 4 37 3 0.0 6 3 38 5 0.0 6 5 39 3 0.0 6 3 40 10 0.0 6 10 41 11 0.0 6 11 42 2 0.0 6 2 43 10 0.0 6 8 1 0 0 0 0 1 44 8 0.0 6 8 45 8 0.0 6 8 46 6 0.0 6 6 47 1 0.0 6 1 48 7 0.0 6 7 49 10 0.0 6 10 50 6 0.0 6 5 1 51 7 0.0 6 6 1 52 8 0.0 6 8 53 2 0.0 6 2 54 3 0.0 6 3 55 4 0.0 6 3 1 56 5 0.0 6 5 57 4 0.0 6 3 1 58 6 0.0 6 6 59 8 0.0 6 6 2 60 4 0.0 6 4 61 2 0.0 6 2 62 3 0.0 6 3 63 4 0.0 6 3 1 64 2 0.0 6 2 65 8 0.0 6 7 1 66 9 0.0 6 8 1 67 4 0.0 6 4 68 6 0.0 6 6 69 9 0.0 6 9 70 7 0.0 6 7 71 10 0.0 6 8 2 72 8 0.0 6 8 73 6 0.0 6 6 74 9 0.0 6 7 2 75 14 0.0 6 13 1 76 5 0.0 6 5 77 6 0.0 6 5 0 0 0 0 1 78 5 0.0 6 4 1 79 13 0.0 6 13 80 8 0.0 6 8 81 8 0.0 6 8 82 13 0.0 6 12 1 83 11 0.0 6 10 1 84 6 0.0 6 6 85 14 0.0 6 14 86 13 0.0 6 12 0 0 0 0 0 1 87 9 0.0 6 9 88 7 0.0 6 7 89 4 0.0 6 4 90 11 0.0 6 10 1 91 5 0.0 6 5 92 9 0.0 6 9 93 5 0.0 6 5 94 7 0.0 6 7 95 9 0.0 6 9 96 12 0.0 6 12 97 9 0.0 6 9 98 9 0.0 6 9 99 10 0.0 6 10 100 13 0.0 6 13 101 9 0.0 6 9 102 15 0.0 6 13 2 103 21 0.0 6 20 1 104 12 0.0 6 11 0 0 0 0 0 1 105 12 0.0 6 11 1 106 16 0.0 6 15 1 107 7 0.0 6 7 108 19 0.0 6 18 1 109 21 0.0 6 20 1 110 23 0.0 6 23 111 25 0.0 6 25 112 15 0.0 6 15 113 20 0.0 6 19 1 114 23 0.0 6 22 0 1 115 18 0.0 6 18 116 25 0.0 6 24 1 117 30 0.0 6 28 1 0 1 118 34 0.0 6 32 2 119 10 0.0 6 10 120 19 0.0 6 19 121 16 0.0 6 15 1 122 25 0.0 6 25 123 30 0.0 6 30 124 24 0.0 6 23 0 0 0 0 1 125 25 0.0 6 25 126 19 0.0 6 19 127 36 0.0 6 36 128 28 0.0 6 27 1 129 32 0.0 6 32 130 32 0.0 6 32 131 25 0.0 6 25 132 29 0.0 6 29 133 32 0.0 6 31 1 134 27 0.0 6 24 2 1 135 38 0.0 6 38 136 34 0.0 6 33 1 137 47 0.0 6 46 1 138 56 0.0 6 56 139 65 0.0 6 65 140 66 0.0 6 65 1 141 230 0.0 6 230 142 432 0.0 6 431 1 143 555 0.0 6 555 144 363 0.0 6 363 145 459 0.0 6 457 2 146 496 0.0 6 495 1 147 550 0.0 6 548 2 148 605 0.0 6 602 2 1 149 528 0.0 6 527 1 150 382 0.0 6 380 2 151 645 0.0 6 644 1 152 481 0.0 6 481 153 394 0.0 6 393 1 154 426 0.0 6 426 155 415 0.0 6 412 3 156 286 0.0 6 286 157 351 0.0 6 351 158 372 0.0 6 371 0 1 159 541 0.0 6 540 1 160 391 0.0 6 390 1 161 424 0.0 6 424 162 603 0.0 6 601 2 163 395 0.0 6 394 1 164 410 0.0 6 407 3 165 354 0.0 6 354 166 397 0.0 6 395 2 167 336 0.0 6 332 3 0 1 168 275 0.0 6 273 2 169 264 0.0 6 262 1 0 0 0 0 1 170 295 0.0 6 293 2 171 400 0.0 6 398 2 172 570 0.0 6 569 1 173 448 0.0 6 446 2 174 465 0.0 6 463 2 175 372 0.0 6 372 176 438 0.0 6 435 3 177 384 0.0 6 381 3 178 441 0.0 6 440 1 179 1537 0.0 6 1536 1 180 498 0.0 6 498 181 419 0.0 6 418 1 182 550 0.0 6 548 2 183 342 0.0 6 338 4 184 365 0.0 6 364 0 0 0 1 185 652 0.0 6 651 0 1 186 412 0.0 6 411 1 187 325 0.0 6 320 5 188 471 0.0 6 468 3 189 540 0.0 6 540 190 360 0.0 6 359 1 191 499 0.0 6 496 3 192 504 0.0 6 501 3 193 601 0.0 6 598 3 194 747 0.0 6 744 2 0 0 0 1 195 679 0.0 6 676 3 196 715 0.0 6 706 8 0 1 197 986 0.0 6 982 4 198 650 0.0 6 643 4 1 1 0 0 1 199 614 0.0 6 611 3 200 565 0.0 6 562 3 201 592 0.0 6 587 2 1 0 0 1 1 202 617 0.0 6 615 2 203 950 0.0 6 947 2 0 1 204 610 0.0 6 606 3 1 205 864 0.0 6 861 3 206 779 0.0 6 776 2 1 207 751 0.0 6 750 1 208 648 0.0 6 648 209 630 0.0 6 627 3 210 817 0.0 6 815 2 211 842 0.0 6 840 2 212 806 0.0 6 803 2 0 0 0 0 1 213 818 0.0 6 812 5 0 0 0 0 1 214 964 0.0 6 957 7 215 667 0.0 6 662 4 0 0 0 1 216 736 0.0 6 733 2 0 0 0 0 1 217 640 0.0 6 635 3 1 0 0 1 218 647 0.0 6 644 3 219 501 0.0 6 499 2 220 553 0.0 6 553 221 631 0.0 6 627 4 222 525 0.0 6 522 2 0 0 1 223 534 0.0 6 532 2 224 739 0.0 6 737 1 0 1 225 662 0.0 6 656 5 0 0 0 1 226 625 0.0 6 623 2 227 525 0.0 6 522 3 228 445 0.0 6 440 5 229 498 0.0 6 495 2 0 0 0 0 1 230 449 0.0 6 445 4 231 497 0.0 6 494 2 1 232 507 0.0 6 504 3 233 452 0.0 6 446 6 234 398 0.0 6 397 1 235 393 0.0 6 391 2 236 441 0.0 6 440 1 237 425 0.0 6 422 3 238 355 0.0 6 355 239 435 0.0 6 429 5 1 240 372 0.0 6 370 1 1 241 381 0.0 6 379 2 242 339 0.0 6 339 243 327 0.0 6 324 3 244 261 0.0 6 260 1 245 249 0.0 6 248 1 246 290 0.0 6 288 2 247 231 0.0 6 230 1 248 212 0.0 6 210 2 249 192 0.0 6 192 250 200 0.0 6 199 1 251 193 0.0 6 190 3 252 192 0.0 6 191 0 1 253 161 0.0 6 157 3 1 254 174 0.0 6 174 255 172 0.0 6 170 2 256 181 0.0 6 181 257 197 0.0 6 192 5 258 168 0.0 6 167 0 0 0 0 1 259 138 0.0 6 135 3 260 124 0.0 6 121 3 261 133 0.0 6 132 0 0 0 0 0 1 262 117 0.0 6 116 1 263 112 0.0 6 109 3 264 105 0.0 6 105 265 105 0.0 6 102 3 266 122 0.0 6 122 267 93 0.0 6 93 268 114 0.0 6 114 269 91 0.0 6 90 1 270 82 0.0 6 81 1 271 75 0.0 6 74 1 272 132 0.0 6 130 2 273 133 0.0 6 133 274 124 0.0 6 122 2 275 180 0.0 6 178 1 0 0 0 1 276 254 0.0 6 249 5 277 680 0.0 6 672 7 0 0 1 278 1399 0.0 6 1388 10 1 279 35 0.0 6 34 1 280 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1748 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 2 0.0 6 2 64 58 0.0 6 58 65 1 0.0 6 0 1 72 2 0.0 6 2 73 6 0.0 6 6 74 14 0.0 6 14 75 21 0.0 6 21 76 9 0.0 6 9 77 22 0.0 6 22 78 15 0.0 6 15 79 19 0.0 6 19 80 18 0.0 6 18 81 14 0.0 6 14 82 18 0.0 6 18 83 20 0.0 6 20 84 14 0.0 6 14 85 15 0.0 6 15 86 17 0.0 6 17 87 7 0.0 6 7 88 4 0.0 6 4 89 14 0.0 6 14 90 17 0.0 6 17 91 12 0.0 6 12 92 8 0.0 6 8 93 14 0.0 6 14 94 18 0.0 6 18 95 5 0.0 6 5 96 14 0.0 6 14 97 7 0.0 6 7 98 7 0.0 6 7 99 9 0.0 6 9 100 7 0.0 6 7 101 3 0.0 6 3 102 4 0.0 6 4 103 12 0.0 6 12 104 15 0.0 6 15 105 8 0.0 6 8 106 5 0.0 6 5 107 8 0.0 6 8 108 7 0.0 6 7 109 5 0.0 6 5 110 23 0.0 6 23 111 43 0.0 6 43 112 11 0.0 6 11 113 12 0.0 6 12 114 10 0.0 6 10 115 8 0.0 6 8 116 7 0.0 6 7 117 14 0.0 6 14 118 2 0.0 6 2 119 8 0.0 6 8 120 9 0.0 6 9 121 7 0.0 6 7 122 3 0.0 6 2 1 123 7 0.0 6 7 124 13 0.0 6 13 125 12 0.0 6 12 126 12 0.0 6 12 127 12 0.0 6 12 128 6 0.0 6 6 129 8 0.0 6 8 130 10 0.0 6 10 131 9 0.0 6 9 132 7 0.0 6 7 133 12 0.0 6 12 134 12 0.0 6 12 135 22 0.0 6 22 136 7 0.0 6 7 137 8 0.0 6 8 138 9 0.0 6 9 139 19 0.0 6 19 140 15 0.0 6 15 141 10 0.0 6 10 142 11 0.0 6 11 143 10 0.0 6 10 144 13 0.0 6 13 145 15 0.0 6 15 146 11 0.0 6 11 147 6 0.0 6 6 148 12 0.0 6 12 149 15 0.0 6 15 150 10 0.0 6 10 151 5 0.0 6 5 152 4 0.0 6 4 153 11 0.0 6 11 154 16 0.0 6 16 155 8 0.0 6 8 156 10 0.0 6 10 157 16 0.0 6 16 158 16 0.0 6 16 159 15 0.0 6 15 160 11 0.0 6 11 161 16 0.0 6 16 162 8 0.0 6 8 163 15 0.0 6 15 164 21 0.0 6 21 165 16 0.0 6 16 166 6 0.0 6 6 167 9 0.0 6 9 168 14 0.0 6 14 169 6 0.0 6 6 170 14 0.0 6 14 171 19 0.0 6 19 172 13 0.0 6 13 173 7 0.0 6 7 174 15 0.0 6 15 175 13 0.0 6 13 176 14 0.0 6 14 177 10 0.0 6 10 178 16 0.0 6 16 179 11 0.0 6 11 180 9 0.0 6 9 181 5 0.0 6 5 182 10 0.0 6 10 183 8 0.0 6 8 184 9 0.0 6 9 185 11 0.0 6 11 186 6 0.0 6 6 187 4 0.0 6 4 188 10 0.0 6 10 189 19 0.0 6 19 190 17 0.0 6 17 191 13 0.0 6 13 192 14 0.0 6 14 193 7 0.0 6 6 1 194 7 0.0 6 7 195 11 0.0 6 11 196 3 0.0 6 3 197 2 0.0 6 2 198 5 0.0 6 5 199 6 0.0 6 5 1 200 6 0.0 6 6 201 14 0.0 6 13 0 0 0 0 0 1 202 2 0.0 6 2 203 8 0.0 6 8 204 17 0.0 6 17 205 4 0.0 6 4 206 5 0.0 6 5 207 2 0.0 6 2 208 4 0.0 6 4 209 10 0.0 6 10 210 3 0.0 6 3 211 4 0.0 6 4 212 5 0.0 6 5 213 4 0.0 6 4 214 5 0.0 6 5 215 6 0.0 6 6 216 2 0.0 6 2 217 2 0.0 6 2 218 3 0.0 6 3 219 1 0.0 6 1 220 6 0.0 6 6 221 7 0.0 6 7 222 2 0.0 6 2 223 2 0.0 6 2 224 7 0.0 6 7 225 3 0.0 6 3 226 12 0.0 6 12 227 8 0.0 6 8 228 7 0.0 6 7 229 2 0.0 6 2 230 2 0.0 6 2 231 2 0.0 6 2 232 4 0.0 6 3 1 233 3 0.0 6 3 234 1 0.0 6 1 235 4 0.0 6 4 236 4 0.0 6 4 237 3 0.0 6 3 238 1 0.0 6 1 239 2 0.0 6 2 240 2 0.0 6 2 242 4 0.0 6 4 243 3 0.0 6 3 244 1 0.0 6 1 245 1 0.0 6 1 247 2 0.0 6 2 249 4 0.0 6 4 250 2 0.0 6 2 251 5 0.0 6 5 252 1 0.0 6 1 253 3 0.0 6 3 254 2 0.0 6 2 255 2 0.0 6 2 257 1 0.0 6 1 258 1 0.0 6 1 259 3 0.0 6 3 260 1 0.0 6 1 261 2 0.0 6 2 262 3 0.0 6 3 263 5 0.0 6 5 265 2 0.0 6 2 268 1 0.0 6 1 272 1 0.0 6 1 273 1 0.0 6 1 274 1 0.0 6 1 277 1 0.0 6 1 278 1 0.0 6 1 279 1 0.0 6 1 280 2 0.0 6 2 281 1 0.0 6 1 284 1 0.0 6 1 291 1 0.0 6 1 302 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 64026 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 64.2 1 1 7 1 4.0 2 0 1 9 2 0.3 2 2 11 2 0.0 3 2 12 6 0.0 3 6 13 11 0.0 3 10 1 14 3 0.0 4 3 15 2 0.0 4 1 1 16 9 0.0 4 8 1 17 7 0.0 5 7 18 14 0.0 5 13 1 19 18 0.0 5 18 20 107 0.0 6 103 4 21 49 0.0 6 49 22 30 0.0 6 30 23 24 0.0 6 21 3 24 18 0.0 6 18 25 33 0.0 6 29 4 26 18 0.0 6 18 27 33 0.0 6 33 28 14 0.0 6 13 1 29 15 0.0 6 15 30 11 0.0 6 8 2 0 1 31 18 0.0 6 17 1 32 17 0.0 6 16 1 33 9 0.0 6 9 34 11 0.0 6 10 0 0 0 0 1 35 16 0.0 6 14 2 36 9 0.0 6 9 37 12 0.0 6 10 2 38 9 0.0 6 8 1 39 3 0.0 6 3 40 10 0.0 6 8 1 0 0 1 41 5 0.0 6 5 42 13 0.0 6 13 43 14 0.0 6 13 1 44 13 0.0 6 13 45 11 0.0 6 10 1 46 17 0.0 6 14 2 0 0 0 1 47 21 0.0 6 18 3 48 15 0.0 6 15 49 14 0.0 6 13 1 50 23 0.0 6 20 1 0 2 51 15 0.0 6 14 1 52 10 0.0 6 8 2 53 34 0.0 6 30 2 2 54 18 0.0 6 18 55 18 0.0 6 17 1 56 21 0.0 6 19 2 57 22 0.0 6 20 1 0 1 58 29 0.0 6 29 59 17 0.0 6 16 1 60 22 0.0 6 22 61 17 0.0 6 16 1 62 35 0.0 6 33 2 63 15 0.0 6 15 64 29 0.0 6 28 1 65 40 0.0 6 37 3 66 37 0.0 6 35 1 0 0 0 0 1 67 36 0.0 6 33 2 1 68 54 0.0 6 54 69 123 0.0 6 120 3 70 53 0.0 6 47 4 0 1 0 1 71 49 0.0 6 49 72 37 0.0 6 36 1 73 54 0.0 6 52 2 74 32 0.0 6 30 2 75 49 0.0 6 43 4 2 76 54 0.0 6 53 1 77 42 0.0 6 38 3 0 0 0 1 78 31 0.0 6 31 79 58 0.0 6 56 2 80 38 0.0 6 38 81 47 0.0 6 43 3 1 82 53 0.0 6 51 2 83 45 0.0 6 43 2 84 45 0.0 6 41 4 85 62 0.0 6 62 86 49 0.0 6 48 0 1 87 50 0.0 6 47 2 0 0 1 88 36 0.0 6 32 2 0 1 1 89 67 0.0 6 65 1 0 0 0 1 90 41 0.0 6 40 1 91 57 0.0 6 55 2 92 50 0.0 6 49 1 93 69 0.0 6 67 2 94 66 0.0 6 65 1 95 90 0.0 6 86 3 0 0 1 96 44 0.0 6 42 1 1 97 76 0.0 6 71 5 98 82 0.0 6 79 3 99 62 0.0 6 61 1 100 82 0.0 6 79 1 0 0 1 1 101 68 0.0 6 67 1 102 68 0.0 6 67 0 0 0 0 1 103 99 0.0 6 97 2 104 104 0.0 6 100 3 1 105 100 0.0 6 97 1 1 1 106 185 0.0 6 178 3 1 0 2 0 1 107 93 0.0 6 90 2 0 1 108 99 0.0 6 95 3 0 0 0 1 109 100 0.0 6 99 1 110 113 0.0 6 111 1 1 111 122 0.0 6 120 2 112 119 0.0 6 119 113 116 0.0 6 113 3 114 144 0.0 6 136 6 1 1 115 132 0.0 6 129 3 116 152 0.0 6 149 2 1 117 142 0.0 6 141 1 118 154 0.0 6 150 2 0 0 2 119 144 0.0 6 141 2 0 0 1 120 190 0.0 6 185 3 1 0 1 121 160 0.0 6 155 4 0 0 0 1 122 152 0.0 6 147 4 0 0 0 0 1 123 188 0.0 6 182 5 0 0 1 124 189 0.0 6 188 0 0 0 0 0 1 125 252 0.0 6 246 6 126 226 0.0 6 222 4 127 270 0.0 6 264 6 128 295 0.0 6 289 5 1 129 286 0.0 6 281 5 130 331 0.0 6 325 6 131 353 0.0 6 346 7 132 395 0.0 6 390 4 0 0 0 0 1 133 386 0.0 6 373 11 0 1 0 0 1 134 374 0.0 6 371 3 135 445 0.0 6 439 6 136 450 0.0 6 441 9 137 450 0.0 6 443 7 138 422 0.0 6 415 6 0 0 0 0 1 139 464 0.0 6 456 8 140 507 0.0 6 494 13 141 690 0.0 6 684 6 142 857 0.0 6 849 7 0 0 0 1 143 965 0.0 6 954 10 0 0 0 1 144 865 0.0 6 857 6 0 1 0 1 145 989 0.0 6 979 7 1 1 0 0 1 146 1056 0.0 6 1045 10 0 1 147 1061 0.0 6 1052 7 1 0 0 0 1 148 1140 0.0 6 1126 11 2 0 1 149 1213 0.0 6 1209 3 0 1 150 1161 0.0 6 1146 11 2 2 151 1402 0.0 6 1392 6 0 1 1 0 2 152 1724 0.0 6 1705 15 2 0 2 153 1204 0.0 6 1196 6 0 2 154 1453 0.0 6 1435 13 0 0 2 1 2 155 1457 0.0 6 1439 15 1 0 1 0 1 156 1057 0.0 6 1047 10 157 1228 0.0 6 1217 7 0 1 1 2 158 1162 0.0 6 1148 13 0 0 0 1 159 1316 0.0 6 1306 8 0 0 0 1 1 160 1141 0.0 6 1127 11 1 1 1 161 1066 0.0 6 1056 8 0 0 1 1 162 1198 0.0 6 1183 12 0 0 0 1 2 163 939 0.0 6 929 9 0 0 1 164 808 0.0 6 802 6 165 881 0.0 6 877 4 166 854 0.0 6 845 9 167 781 0.0 6 775 5 0 0 0 0 1 168 960 0.0 6 955 5 169 750 0.0 6 744 4 0 0 0 0 2 170 994 0.0 6 990 3 0 1 171 946 0.0 6 944 2 172 946 0.0 6 936 10 173 906 0.0 6 899 6 0 0 1 174 941 0.0 6 933 7 0 0 0 1 175 941 0.0 6 937 3 0 0 0 0 1 176 871 0.0 6 864 5 1 0 1 177 855 0.0 6 845 7 2 0 1 178 879 0.0 6 873 6 179 851 0.0 6 843 7 0 1 180 679 0.0 6 674 4 1 181 526 0.0 6 520 5 0 0 0 1 182 438 0.0 6 435 3 183 496 0.0 6 494 1 0 0 0 0 1 184 454 0.0 6 445 9 185 703 0.0 6 700 2 1 186 813 0.0 6 808 5 187 678 0.0 6 673 4 0 0 0 0 1 188 496 0.0 6 494 2 189 465 0.0 6 462 2 0 0 1 190 405 0.0 6 401 4 191 415 0.0 6 413 1 1 192 359 0.0 6 358 1 193 342 0.0 6 339 3 194 287 0.0 6 284 3 195 256 0.0 6 252 3 0 1 196 270 0.0 6 263 5 1 0 0 1 197 168 0.0 6 167 1 198 286 0.0 6 284 1 0 0 0 0 1 199 227 0.0 6 227 200 234 0.0 6 232 2 201 178 0.0 6 176 2 202 180 0.0 6 180 203 158 0.0 6 158 204 164 0.0 6 163 1 205 138 0.0 6 137 1 206 145 0.0 6 142 3 207 90 0.0 6 87 3 208 106 0.0 6 106 209 85 0.0 6 84 0 1 210 75 0.0 6 73 1 1 211 89 0.0 6 88 1 212 59 0.0 6 58 1 213 55 0.0 6 55 214 74 0.0 6 74 215 65 0.0 6 63 2 216 101 0.0 6 100 0 1 217 55 0.0 6 54 0 0 0 0 1 218 48 0.0 6 47 0 1 219 41 0.0 6 41 220 30 0.0 6 29 1 221 33 0.0 6 33 222 47 0.0 6 47 223 29 0.0 6 27 2 224 26 0.0 6 25 1 225 16 0.0 6 16 226 25 0.0 6 24 1 227 29 0.0 6 27 2 228 14 0.0 6 14 229 38 0.0 6 36 2 230 83 0.0 6 83 231 20 0.0 6 17 2 0 1 232 24 0.0 6 24 233 8 0.0 6 8 234 24 0.0 6 23 1 235 14 0.0 6 14 236 11 0.0 6 10 1 237 8 0.0 6 8 238 10 0.0 6 10 239 1 0.0 6 1 240 5 0.0 6 5 241 4 0.0 6 4 242 4 0.0 6 4 243 4 0.0 6 4 244 3 0.0 6 3 245 10 0.0 6 9 1 246 4 0.0 6 4 247 3 0.0 6 3 248 1 0.0 6 1 249 7 0.0 6 7 250 1 0.0 6 1 251 9 0.0 6 9 252 6 0.0 6 6 253 6 0.0 6 6 254 2 0.0 6 2 255 2 0.0 6 2 277 1 0.0 6 1 280 10 0.0 6 10 281 53 0.0 6 52 1 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1642 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 66 1 0.0 6 1 67 1 0.0 6 1 69 1 0.0 6 1 70 8 0.0 6 8 71 14 0.0 6 14 72 26 0.0 6 26 73 13 0.0 6 13 74 10 0.0 6 10 75 18 0.0 6 18 76 18 0.0 6 17 1 77 23 0.0 6 23 78 26 0.0 6 26 79 4 0.0 6 4 80 29 0.0 6 29 81 13 0.0 6 13 82 8 0.0 6 8 83 11 0.0 6 11 84 23 0.0 6 23 85 11 0.0 6 11 86 14 0.0 6 14 87 8 0.0 6 8 88 34 0.0 6 33 1 89 13 0.0 6 12 0 0 0 1 90 8 0.0 6 8 91 18 0.0 6 17 1 92 8 0.0 6 7 1 93 14 0.0 6 13 0 0 0 1 94 13 0.0 6 13 95 16 0.0 6 16 96 5 0.0 6 5 97 9 0.0 6 9 98 5 0.0 6 5 99 6 0.0 6 6 100 15 0.0 6 15 101 18 0.0 6 18 102 15 0.0 6 15 103 15 0.0 6 15 104 14 0.0 6 13 0 0 1 105 11 0.0 6 11 106 17 0.0 6 16 1 107 15 0.0 6 15 108 51 0.0 6 51 109 13 0.0 6 13 110 13 0.0 6 13 111 20 0.0 6 20 112 5 0.0 6 5 113 7 0.0 6 7 114 18 0.0 6 18 115 10 0.0 6 10 116 1 0.0 6 1 117 11 0.0 6 10 1 118 6 0.0 6 6 119 6 0.0 6 6 120 12 0.0 6 12 121 43 0.0 6 43 122 11 0.0 6 10 1 123 14 0.0 6 14 124 10 0.0 6 10 125 12 0.0 6 12 126 21 0.0 6 21 127 7 0.0 6 7 128 14 0.0 6 14 129 7 0.0 6 7 130 21 0.0 6 21 131 10 0.0 6 10 132 23 0.0 6 23 133 10 0.0 6 10 134 9 0.0 6 9 135 10 0.0 6 10 136 11 0.0 6 11 137 27 0.0 6 27 138 14 0.0 6 14 139 14 0.0 6 14 140 25 0.0 6 25 141 10 0.0 6 10 142 12 0.0 6 12 143 14 0.0 6 14 144 9 0.0 6 9 145 11 0.0 6 11 146 7 0.0 6 7 147 9 0.0 6 8 0 0 0 1 148 2 0.0 6 2 149 15 0.0 6 15 150 16 0.0 6 16 151 16 0.0 6 16 152 14 0.0 6 14 153 10 0.0 6 9 1 154 17 0.0 6 17 155 19 0.0 6 19 156 15 0.0 6 15 157 11 0.0 6 11 158 15 0.0 6 15 159 7 0.0 6 7 160 12 0.0 6 12 161 22 0.0 6 21 1 162 14 0.0 6 13 0 0 1 163 11 0.0 6 11 164 8 0.0 6 8 165 13 0.0 6 13 166 4 0.0 6 4 167 8 0.0 6 8 168 18 0.0 6 18 169 10 0.0 6 10 170 12 0.0 6 12 171 17 0.0 6 16 1 172 15 0.0 6 15 173 11 0.0 6 11 174 12 0.0 6 12 175 22 0.0 6 22 176 10 0.0 6 10 177 7 0.0 6 7 178 2 0.0 6 2 179 5 0.0 6 5 180 7 0.0 6 7 181 11 0.0 6 11 182 6 0.0 6 6 183 14 0.0 6 14 184 6 0.0 6 5 0 0 0 1 185 3 0.0 6 2 1 186 8 0.0 6 8 187 12 0.0 6 12 188 4 0.0 6 4 189 10 0.0 6 10 190 2 0.0 6 2 191 5 0.0 6 5 192 1 0.0 6 1 193 2 0.0 6 2 195 4 0.0 6 4 196 2 0.0 6 2 197 1 0.0 6 0 1 198 3 0.0 6 3 199 2 0.0 6 2 200 4 0.0 6 4 201 5 0.0 6 5 202 6 0.0 6 6 203 2 0.0 6 2 204 1 0.0 6 1 205 2 0.0 6 2 206 1 0.0 6 1 207 1 0.0 6 1 208 1 0.0 6 1 209 2 0.0 6 2 211 3 0.0 6 2 1 212 2 0.0 6 2 213 1 0.0 6 1 217 2 0.0 6 2 218 1 0.0 6 1 220 1 0.0 6 1 221 1 0.0 6 1 222 1 0.0 6 1 224 1 0.0 6 1 225 1 0.0 6 1 227 1 0.0 6 1 236 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_20_35_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_20_262_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_20_35_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_20_262_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.27 s (29 µs/read; 2.07 M reads/minute). === Summary === Total read pairs processed: 43,867 Read 1 with adapter: 43,863 (100.0%) Read 2 with adapter: 43,835 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 36 (0.1%) Pairs written (passing filters): 43,831 (99.9%) Total basepairs processed: 21,624,106 bp Read 1: 12,265,825 bp Read 2: 9,358,281 bp Total written (filtered): 2,851,612 bp (13.2%) Read 1: 1,447,687 bp Read 2: 1,403,925 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 43827 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 171.4 1 1 6 1 10.7 1 0 0 1 9 1 0.2 2 0 1 12 1 0.0 3 1 14 1 0.0 4 1 17 1 0.0 5 1 19 1 0.0 5 1 20 5 0.0 6 4 1 21 4 0.0 6 4 22 7 0.0 6 7 25 10 0.0 6 10 27 3 0.0 6 3 29 1 0.0 6 1 30 3 0.0 6 3 31 2 0.0 6 2 33 2 0.0 6 2 34 3 0.0 6 3 36 1 0.0 6 1 37 2 0.0 6 2 38 1 0.0 6 1 40 1 0.0 6 1 41 1 0.0 6 1 42 1 0.0 6 1 43 1 0.0 6 1 44 5 0.0 6 5 45 4 0.0 6 4 47 3 0.0 6 2 1 49 4 0.0 6 4 50 1 0.0 6 1 51 1 0.0 6 1 52 4 0.0 6 3 0 1 53 3 0.0 6 2 1 54 3 0.0 6 3 55 2 0.0 6 2 56 3 0.0 6 3 57 4 0.0 6 4 59 2 0.0 6 2 61 3 0.0 6 3 62 1 0.0 6 1 63 3 0.0 6 3 64 8 0.0 6 8 65 3 0.0 6 3 66 1 0.0 6 1 67 2 0.0 6 2 68 1 0.0 6 1 69 3 0.0 6 2 1 72 2 0.0 6 2 73 1 0.0 6 1 74 1 0.0 6 1 75 1 0.0 6 0 1 76 2 0.0 6 2 77 1 0.0 6 1 78 3 0.0 6 2 1 79 2 0.0 6 2 80 4 0.0 6 4 81 2 0.0 6 0 1 0 0 1 83 3 0.0 6 3 84 1 0.0 6 1 86 14 0.0 6 14 87 3 0.0 6 3 89 2 0.0 6 2 90 1 0.0 6 1 94 1 0.0 6 1 97 1 0.0 6 1 98 5 0.0 6 5 99 2 0.0 6 2 101 7 0.0 6 7 102 6 0.0 6 5 1 104 4 0.0 6 4 105 2 0.0 6 2 106 1 0.0 6 1 107 1 0.0 6 1 108 5 0.0 6 4 1 109 3 0.0 6 3 110 2 0.0 6 1 1 111 2 0.0 6 2 112 6 0.0 6 6 113 6 0.0 6 6 114 4 0.0 6 4 115 7 0.0 6 7 116 1 0.0 6 1 117 6 0.0 6 4 2 118 3 0.0 6 3 119 5 0.0 6 4 1 120 6 0.0 6 6 121 7 0.0 6 6 1 122 14 0.0 6 12 2 123 9 0.0 6 8 1 124 8 0.0 6 8 125 9 0.0 6 9 126 5 0.0 6 5 127 5 0.0 6 5 128 14 0.0 6 14 130 3 0.0 6 3 131 2 0.0 6 2 132 5 0.0 6 5 133 4 0.0 6 4 134 9 0.0 6 9 135 13 0.0 6 12 1 136 10 0.0 6 10 137 31 0.0 6 29 1 1 138 32 0.0 6 31 1 139 19 0.0 6 17 2 140 30 0.0 6 28 2 141 19 0.0 6 17 2 142 18 0.0 6 16 2 143 85 0.0 6 83 2 144 19 0.0 6 19 145 117 0.0 6 116 1 146 23 0.0 6 23 147 66 0.0 6 65 1 148 24 0.0 6 23 0 0 1 149 57 0.0 6 56 1 150 31 0.0 6 29 1 1 151 24 0.0 6 24 152 23 0.0 6 21 2 153 11 0.0 6 11 154 19 0.0 6 19 155 17 0.0 6 16 1 156 27 0.0 6 27 157 206 0.0 6 203 2 1 158 40 0.0 6 40 159 44 0.0 6 42 2 160 36 0.0 6 36 161 28 0.0 6 26 2 162 26 0.0 6 25 0 1 163 31 0.0 6 29 2 164 36 0.0 6 36 165 34 0.0 6 32 2 166 13 0.0 6 12 0 1 167 16 0.0 6 16 168 22 0.0 6 22 169 20 0.0 6 18 2 170 21 0.0 6 21 171 19 0.0 6 19 172 15 0.0 6 14 1 173 24 0.0 6 24 174 14 0.0 6 12 2 175 62 0.0 6 60 2 176 33 0.0 6 32 1 177 76 0.0 6 75 1 178 23 0.0 6 21 2 179 42 0.0 6 38 4 180 18 0.0 6 17 1 181 17 0.0 6 17 182 48 0.0 6 47 1 183 83 0.0 6 80 2 1 184 128 0.0 6 123 5 185 127 0.0 6 125 2 186 212 0.0 6 200 12 187 96 0.0 6 96 188 85 0.0 6 81 4 189 62 0.0 6 61 1 190 78 0.0 6 78 191 89 0.0 6 87 2 192 124 0.0 6 118 5 1 193 114 0.0 6 110 4 194 125 0.0 6 120 5 195 169 0.0 6 164 4 1 196 202 0.0 6 197 5 197 214 0.0 6 205 9 198 322 0.0 6 319 3 199 90 0.0 6 88 2 200 118 0.0 6 113 5 201 123 0.0 6 119 4 202 177 0.0 6 171 6 203 189 0.0 6 184 5 204 126 0.0 6 121 5 205 223 0.0 6 221 1 1 206 199 0.0 6 193 5 1 207 306 0.0 6 295 10 1 208 232 0.0 6 224 8 209 248 0.0 6 245 3 210 207 0.0 6 200 7 211 186 0.0 6 180 5 1 212 125 0.0 6 118 7 213 197 0.0 6 189 8 214 206 0.0 6 201 5 215 107 0.0 6 106 1 216 198 0.0 6 187 10 0 0 1 217 330 0.0 6 323 7 218 205 0.0 6 202 3 219 152 0.0 6 146 5 1 220 160 0.0 6 155 4 1 221 129 0.0 6 122 6 0 1 222 270 0.0 6 260 10 223 109 0.0 6 105 3 1 224 148 0.0 6 145 3 225 232 0.0 6 222 10 226 582 0.0 6 569 13 227 477 0.0 6 465 12 228 424 0.0 6 414 10 229 334 0.0 6 325 9 230 359 0.0 6 352 7 231 303 0.0 6 295 7 1 232 437 0.0 6 415 21 1 233 372 0.0 6 362 10 234 258 0.0 6 249 9 235 123 0.0 6 116 7 236 167 0.0 6 163 3 1 237 138 0.0 6 137 1 238 261 0.0 6 259 2 239 301 0.0 6 291 9 1 240 367 0.0 6 355 12 241 537 0.0 6 522 15 242 550 0.0 6 540 10 243 440 0.0 6 432 8 244 382 0.0 6 372 10 245 534 0.0 6 524 10 246 200 0.0 6 193 7 247 352 0.0 6 341 10 0 1 248 135 0.0 6 135 249 592 0.0 6 573 19 250 324 0.0 6 313 11 251 428 0.0 6 418 10 252 352 0.0 6 345 7 253 205 0.0 6 192 12 1 254 660 0.0 6 646 14 255 423 0.0 6 417 6 256 311 0.0 6 300 11 257 416 0.0 6 393 23 258 636 0.0 6 619 17 259 612 0.0 6 597 15 260 733 0.0 6 711 22 261 310 0.0 6 304 6 262 639 0.0 6 624 15 263 1252 0.0 6 1233 18 1 264 607 0.0 6 584 23 265 906 0.0 6 893 13 266 470 0.0 6 454 16 267 897 0.0 6 873 24 268 780 0.0 6 758 21 1 269 500 0.0 6 490 10 270 208 0.0 6 202 5 1 271 604 0.0 6 587 16 1 272 2003 0.0 6 1963 40 273 1059 0.0 6 1035 23 1 274 1206 0.0 6 1176 30 275 984 0.0 6 949 35 276 1709 0.0 6 1661 47 1 277 4862 0.0 6 4764 93 3 2 278 1698 0.0 6 1604 88 5 1 279 137 0.0 6 127 9 1 280 28 0.0 6 25 3 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 36 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 73 2 0.0 6 2 74 3 0.0 6 3 75 2 0.0 6 2 76 1 0.0 6 1 77 1 0.0 6 1 86 1 0.0 6 1 87 3 0.0 6 3 106 1 0.0 6 1 107 2 0.0 6 2 108 1 0.0 6 1 127 1 0.0 6 1 128 5 0.0 6 5 129 1 0.0 6 1 175 1 0.0 6 1 207 3 0.0 6 3 208 6 0.0 6 6 226 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 43797 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 685.4 0 1 1 6 1 10.7 1 0 1 9 2 0.2 2 2 10 1 0.0 3 1 11 4 0.0 3 2 2 13 9 0.0 3 6 3 14 7 0.0 4 6 1 16 12 0.0 4 12 17 11 0.0 5 7 2 1 1 18 13 0.0 5 12 1 19 5 0.0 5 2 3 20 31 0.0 6 31 21 44 0.0 6 42 2 22 27 0.0 6 25 2 23 18 0.0 6 18 24 20 0.0 6 20 25 10 0.0 6 8 2 26 9 0.0 6 9 27 15 0.0 6 14 1 28 8 0.0 6 8 29 10 0.0 6 8 2 30 10 0.0 6 9 1 31 6 0.0 6 6 32 4 0.0 6 4 33 6 0.0 6 6 34 8 0.0 6 7 0 0 0 0 1 35 5 0.0 6 5 36 4 0.0 6 4 37 8 0.0 6 6 2 38 6 0.0 6 5 1 39 8 0.0 6 8 40 1 0.0 6 1 41 6 0.0 6 6 42 6 0.0 6 6 43 15 0.0 6 13 1 0 0 1 44 19 0.0 6 17 2 45 4 0.0 6 3 1 46 17 0.0 6 13 4 47 14 0.0 6 12 2 48 14 0.0 6 12 2 49 5 0.0 6 5 50 10 0.0 6 10 51 8 0.0 6 7 1 52 21 0.0 6 21 53 6 0.0 6 6 54 15 0.0 6 14 1 55 15 0.0 6 14 1 56 11 0.0 6 9 2 57 18 0.0 6 16 2 58 17 0.0 6 16 1 59 6 0.0 6 5 1 60 12 0.0 6 12 61 11 0.0 6 11 62 3 0.0 6 3 63 4 0.0 6 3 1 64 8 0.0 6 7 1 65 6 0.0 6 5 1 66 21 0.0 6 18 3 67 90 0.0 6 89 1 68 24 0.0 6 22 2 69 31 0.0 6 28 2 0 0 0 1 70 12 0.0 6 11 0 0 0 1 71 21 0.0 6 20 1 72 21 0.0 6 20 1 74 4 0.0 6 3 0 0 0 1 75 9 0.0 6 8 1 76 12 0.0 6 12 77 8 0.0 6 6 2 78 16 0.0 6 13 3 79 21 0.0 6 19 1 0 1 80 10 0.0 6 9 0 1 81 11 0.0 6 9 2 82 24 0.0 6 19 5 83 44 0.0 6 42 2 84 26 0.0 6 25 1 85 18 0.0 6 17 1 86 11 0.0 6 10 1 87 44 0.0 6 38 5 1 88 16 0.0 6 15 0 0 1 89 24 0.0 6 21 3 90 27 0.0 6 27 91 26 0.0 6 25 1 92 16 0.0 6 16 93 46 0.0 6 40 4 1 0 1 94 36 0.0 6 35 1 95 35 0.0 6 31 3 0 1 96 30 0.0 6 27 3 97 28 0.0 6 28 98 28 0.0 6 28 99 34 0.0 6 33 1 100 26 0.0 6 24 2 101 33 0.0 6 29 4 102 48 0.0 6 45 3 103 65 0.0 6 63 2 104 55 0.0 6 51 3 0 0 1 105 39 0.0 6 37 2 106 31 0.0 6 30 1 107 28 0.0 6 28 108 33 0.0 6 31 2 109 29 0.0 6 28 1 110 31 0.0 6 30 0 1 111 23 0.0 6 21 1 1 112 30 0.0 6 28 0 2 113 29 0.0 6 26 3 114 43 0.0 6 40 3 115 36 0.0 6 34 1 1 116 53 0.0 6 50 2 1 117 35 0.0 6 33 2 118 37 0.0 6 36 1 119 34 0.0 6 31 3 120 66 0.0 6 58 8 121 56 0.0 6 54 2 122 78 0.0 6 73 4 1 123 60 0.0 6 56 4 124 100 0.0 6 96 3 1 125 63 0.0 6 58 4 1 126 72 0.0 6 65 6 0 0 1 127 59 0.0 6 57 1 1 128 42 0.0 6 41 1 129 25 0.0 6 25 130 59 0.0 6 58 1 131 114 0.0 6 112 2 132 161 0.0 6 152 6 2 0 1 133 111 0.0 6 107 4 134 172 0.0 6 162 10 135 264 0.0 6 258 6 136 77 0.0 6 73 4 137 150 0.0 6 145 4 1 138 75 0.0 6 72 3 139 125 0.0 6 121 4 140 180 0.0 6 176 4 141 199 0.0 6 192 6 0 1 142 261 0.0 6 250 9 2 143 256 0.0 6 252 4 144 326 0.0 6 316 9 0 0 1 145 190 0.0 6 182 8 146 137 0.0 6 125 11 1 147 117 0.0 6 113 4 148 168 0.0 6 158 8 2 149 284 0.0 6 277 7 150 469 0.0 6 453 15 1 151 169 0.0 6 164 4 1 152 501 0.0 6 492 9 153 246 0.0 6 240 6 154 310 0.0 6 300 9 0 0 1 155 345 0.0 6 341 4 156 200 0.0 6 196 4 157 348 0.0 6 341 6 0 1 158 345 0.0 6 334 9 0 0 2 159 150 0.0 6 147 3 160 287 0.0 6 280 6 0 1 161 177 0.0 6 173 4 162 152 0.0 6 141 11 163 263 0.0 6 256 7 164 258 0.0 6 249 8 1 165 272 0.0 6 265 7 166 385 0.0 6 373 11 1 167 193 0.0 6 183 10 168 574 0.0 6 559 15 169 175 0.0 6 166 9 170 244 0.0 6 233 11 171 564 0.0 6 535 29 172 147 0.0 6 139 8 173 264 0.0 6 258 6 174 393 0.0 6 380 12 1 175 649 0.0 6 628 18 2 0 1 176 369 0.0 6 357 11 1 177 381 0.0 6 370 9 1 1 178 368 0.0 6 355 12 1 179 467 0.0 6 444 23 180 369 0.0 6 348 20 1 181 524 0.0 6 502 21 0 0 1 182 115 0.0 6 108 6 0 0 1 183 392 0.0 6 375 16 1 184 440 0.0 6 428 12 185 532 0.0 6 513 18 1 186 1019 0.0 6 983 32 1 0 3 187 881 0.0 6 847 32 0 1 1 188 841 0.0 6 812 28 1 189 835 0.0 6 806 28 1 190 1142 0.0 6 1109 33 191 1115 0.0 6 1072 39 2 0 2 192 652 0.0 6 630 21 1 193 400 0.0 6 387 10 1 0 2 194 763 0.0 6 740 22 1 195 1140 0.0 6 1099 40 0 1 196 2144 0.0 6 2093 51 197 538 0.0 6 513 23 2 198 1170 0.0 6 1132 36 2 199 2072 0.0 6 2008 57 4 0 3 200 625 0.0 6 593 30 2 201 883 0.0 6 854 26 3 202 477 0.0 6 466 10 0 0 1 203 449 0.0 6 441 8 204 572 0.0 6 560 12 205 243 0.0 6 233 10 206 214 0.0 6 207 6 0 1 207 119 0.0 6 117 2 208 241 0.0 6 238 3 209 202 0.0 6 195 6 0 0 1 210 190 0.0 6 186 4 211 171 0.0 6 167 4 212 325 0.0 6 316 8 1 213 147 0.0 6 140 6 0 0 1 214 475 0.0 6 467 8 215 354 0.0 6 348 5 1 216 555 0.0 6 549 6 217 211 0.0 6 201 9 0 0 1 218 224 0.0 6 218 6 219 207 0.0 6 202 5 220 63 0.0 6 61 2 221 215 0.0 6 207 7 1 222 369 0.0 6 359 10 223 502 0.0 6 494 8 224 133 0.0 6 132 1 225 272 0.0 6 270 2 226 117 0.0 6 115 2 227 207 0.0 6 203 4 228 92 0.0 6 90 2 229 254 0.0 6 251 3 230 172 0.0 6 167 5 231 162 0.0 6 156 6 232 283 0.0 6 270 13 233 135 0.0 6 133 2 234 91 0.0 6 89 2 235 46 0.0 6 46 236 10 0.0 6 9 1 237 11 0.0 6 11 238 41 0.0 6 41 239 23 0.0 6 23 240 69 0.0 6 69 241 89 0.0 6 89 242 150 0.0 6 146 3 1 243 129 0.0 6 126 3 244 77 0.0 6 76 1 245 29 0.0 6 28 1 246 39 0.0 6 38 1 247 20 0.0 6 20 248 40 0.0 6 39 1 249 16 0.0 6 15 1 250 11 0.0 6 10 1 251 19 0.0 6 17 2 252 6 0.0 6 6 253 3 0.0 6 3 258 1 0.0 6 1 277 7 0.0 6 7 278 32 0.0 6 31 1 279 24 0.0 6 24 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 38 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 2 0.0 6 2 72 2 0.0 6 2 73 1 0.0 6 1 74 4 0.0 6 4 75 1 0.0 6 1 76 1 0.0 6 1 84 1 0.0 6 1 85 2 0.0 6 2 86 7 0.0 6 7 106 1 0.0 6 1 115 1 0.0 6 1 125 1 0.0 6 1 127 10 0.0 6 10 189 2 0.0 6 2 206 1 0.0 6 1 207 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_01_24_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_01_251_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_01_24_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_01_251_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_02_25_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_02_252_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_02_25_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_02_252_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_03_26_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_03_253_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_03_26_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_03_253_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_04_27_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_04_254_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_04_27_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_04_254_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_05_28_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_05_255_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_05_28_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_05_255_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_06_29_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_06_256_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_06_29_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_06_256_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_15_30_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_15_257_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_15_30_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_15_257_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_16_31_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_16_258_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_16_31_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_16_258_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_17_32_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_17_259_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_17_32_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_17_259_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_18_33_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_18_260_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_18_33_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_18_260_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_19_34_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_19_261_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_19_34_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_19_261_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_20_35_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_03-Rev_20_262_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_20_35_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_03-Rev_20_262_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_01_36_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_01_263_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_01_36_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_01_263_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.39 s (25 µs/read; 2.41 M reads/minute). === Summary === Total read pairs processed: 55,732 Read 1 with adapter: 55,726 (100.0%) Read 2 with adapter: 55,689 (99.9%) == Read fate breakdown == Pairs that were too short: 36 (0.1%) Pairs discarded as untrimmed: 48 (0.1%) Pairs written (passing filters): 55,648 (99.8%) Total basepairs processed: 24,366,601 bp Read 1: 14,037,664 bp Read 2: 10,328,937 bp Total written (filtered): 3,400,728 bp (14.0%) Read 1: 1,715,983 bp Read 2: 1,684,745 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 54515 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 870.8 0 1 4 2 217.7 1 0 2 5 1 54.4 1 1 6 2 13.6 1 0 0 2 9 2 0.2 2 2 10 1 0.1 3 1 12 1 0.0 3 1 13 1 0.0 3 1 14 1 0.0 4 1 15 4 0.0 4 4 16 6 0.0 4 5 1 17 1 0.0 5 1 19 2 0.0 5 1 1 20 92 0.0 6 88 4 21 42 0.0 6 41 1 22 28 0.0 6 24 3 1 23 21 0.0 6 20 1 24 14 0.0 6 13 1 25 17 0.0 6 17 26 14 0.0 6 13 0 0 0 0 0 1 27 8 0.0 6 7 1 28 9 0.0 6 9 29 9 0.0 6 8 0 0 0 0 1 30 7 0.0 6 7 31 2 0.0 6 2 32 4 0.0 6 4 33 7 0.0 6 7 34 3 0.0 6 3 35 4 0.0 6 3 1 36 7 0.0 6 7 37 2 0.0 6 2 38 2 0.0 6 2 39 1 0.0 6 1 40 2 0.0 6 2 41 5 0.0 6 4 1 42 7 0.0 6 7 43 7 0.0 6 7 44 7 0.0 6 7 45 3 0.0 6 3 46 4 0.0 6 4 47 4 0.0 6 4 48 2 0.0 6 2 49 5 0.0 6 5 50 6 0.0 6 6 51 12 0.0 6 11 1 52 5 0.0 6 5 53 6 0.0 6 6 54 4 0.0 6 4 55 8 0.0 6 8 56 5 0.0 6 5 57 2 0.0 6 1 0 0 1 58 5 0.0 6 5 59 2 0.0 6 2 60 1 0.0 6 1 61 5 0.0 6 5 62 3 0.0 6 3 63 6 0.0 6 6 64 6 0.0 6 5 1 65 5 0.0 6 4 1 66 4 0.0 6 4 67 6 0.0 6 5 1 68 4 0.0 6 4 69 5 0.0 6 5 70 9 0.0 6 9 71 6 0.0 6 5 1 72 9 0.0 6 8 1 73 10 0.0 6 10 74 12 0.0 6 12 75 6 0.0 6 6 76 15 0.0 6 15 77 11 0.0 6 10 1 78 10 0.0 6 10 79 9 0.0 6 8 1 80 4 0.0 6 4 81 5 0.0 6 3 2 82 5 0.0 6 5 83 2 0.0 6 2 84 10 0.0 6 10 85 10 0.0 6 10 86 8 0.0 6 8 87 12 0.0 6 11 1 88 4 0.0 6 4 89 6 0.0 6 4 1 0 0 1 90 4 0.0 6 4 91 6 0.0 6 5 0 0 1 92 2 0.0 6 2 93 6 0.0 6 5 1 94 6 0.0 6 6 95 3 0.0 6 3 96 3 0.0 6 3 97 10 0.0 6 10 98 5 0.0 6 5 99 7 0.0 6 7 100 9 0.0 6 9 101 11 0.0 6 11 102 9 0.0 6 8 1 103 12 0.0 6 12 104 14 0.0 6 14 105 10 0.0 6 9 1 106 19 0.0 6 18 1 107 23 0.0 6 22 1 108 14 0.0 6 14 109 17 0.0 6 16 1 110 9 0.0 6 9 111 5 0.0 6 5 112 33 0.0 6 33 113 18 0.0 6 18 114 25 0.0 6 23 2 115 10 0.0 6 10 116 12 0.0 6 12 117 16 0.0 6 15 0 0 0 0 1 118 11 0.0 6 11 119 22 0.0 6 22 120 18 0.0 6 18 121 21 0.0 6 18 3 122 21 0.0 6 21 123 18 0.0 6 18 124 13 0.0 6 13 125 27 0.0 6 26 1 126 16 0.0 6 16 127 17 0.0 6 17 128 15 0.0 6 15 129 14 0.0 6 14 130 27 0.0 6 27 131 24 0.0 6 24 132 27 0.0 6 27 133 17 0.0 6 16 0 1 134 25 0.0 6 25 135 21 0.0 6 20 1 136 19 0.0 6 19 137 20 0.0 6 19 1 138 25 0.0 6 24 1 139 28 0.0 6 27 1 140 36 0.0 6 36 141 133 0.0 6 133 142 208 0.0 6 208 143 226 0.0 6 226 144 165 0.0 6 164 1 145 120 0.0 6 111 8 0 1 146 178 0.0 6 177 1 147 178 0.0 6 178 148 127 0.0 6 126 0 0 0 0 1 149 127 0.0 6 125 1 0 1 150 122 0.0 6 122 151 120 0.0 6 117 3 152 109 0.0 6 108 1 153 121 0.0 6 121 154 108 0.0 6 108 155 116 0.0 6 112 3 0 1 156 107 0.0 6 105 2 157 92 0.0 6 91 1 158 151 0.0 6 148 0 2 0 0 0 1 159 139 0.0 6 139 160 98 0.0 6 97 1 161 148 0.0 6 145 2 0 0 1 162 165 0.0 6 163 2 163 128 0.0 6 123 5 164 143 0.0 6 141 2 165 136 0.0 6 136 166 156 0.0 6 154 1 0 0 0 0 1 167 163 0.0 6 162 1 168 148 0.0 6 147 1 169 154 0.0 6 152 2 170 206 0.0 6 202 4 171 155 0.0 6 155 172 133 0.0 6 128 4 0 0 1 173 163 0.0 6 162 1 174 167 0.0 6 163 4 175 181 0.0 6 178 2 0 1 176 181 0.0 6 178 2 1 177 204 0.0 6 200 3 0 0 0 1 178 201 0.0 6 198 3 179 390 0.0 6 386 4 180 211 0.0 6 210 1 181 177 0.0 6 173 3 1 182 164 0.0 6 161 1 0 2 183 189 0.0 6 188 1 184 162 0.0 6 160 2 185 210 0.0 6 202 7 0 1 186 226 0.0 6 219 5 1 1 187 228 0.0 6 225 3 188 246 0.0 6 243 3 189 228 0.0 6 224 3 0 0 0 1 190 198 0.0 6 192 6 191 223 0.0 6 219 4 192 275 0.0 6 270 4 0 1 193 264 0.0 6 257 6 1 194 359 0.0 6 354 3 1 1 195 327 0.0 6 324 3 196 347 0.0 6 344 2 0 0 0 1 197 442 0.0 6 434 7 1 198 486 0.0 6 476 9 1 199 488 0.0 6 477 7 2 0 1 1 200 569 0.0 6 558 11 201 449 0.0 6 437 9 2 1 202 392 0.0 6 387 5 203 507 0.0 6 501 6 204 425 0.0 6 421 3 1 205 487 0.0 6 479 7 0 0 0 0 1 206 448 0.0 6 438 10 207 507 0.0 6 499 7 1 208 663 0.0 6 647 12 3 0 0 1 209 510 0.0 6 503 7 210 597 0.0 6 583 14 211 416 0.0 6 408 8 212 463 0.0 6 453 6 1 0 0 2 1 213 572 0.0 6 553 18 1 214 641 0.0 6 632 6 0 2 0 1 215 657 0.0 6 649 6 1 1 216 731 0.0 6 721 9 0 1 217 858 0.0 6 845 9 1 1 2 218 714 0.0 6 705 9 219 679 0.0 6 666 11 1 0 1 220 693 0.0 6 681 11 0 0 1 221 637 0.0 6 629 7 0 1 222 515 0.0 6 509 5 1 223 591 0.0 6 582 6 0 1 0 2 224 632 0.0 6 631 0 0 0 0 1 225 656 0.0 6 644 11 1 226 611 0.0 6 607 3 1 227 689 0.0 6 682 6 1 228 651 0.0 6 640 9 0 0 0 0 2 229 594 0.0 6 584 10 230 513 0.0 6 509 3 1 231 549 0.0 6 547 2 232 577 0.0 6 574 1 0 0 0 1 1 233 553 0.0 6 547 6 234 511 0.0 6 503 8 235 504 0.0 6 499 5 236 531 0.0 6 524 6 0 1 237 494 0.0 6 486 7 0 0 0 1 238 515 0.0 6 513 2 239 558 0.0 6 555 2 0 1 240 515 0.0 6 510 4 1 241 509 0.0 6 502 6 0 0 1 242 429 0.0 6 424 4 0 0 0 0 1 243 506 0.0 6 501 4 0 1 244 479 0.0 6 473 6 245 472 0.0 6 468 4 246 436 0.0 6 433 3 247 448 0.0 6 442 6 248 321 0.0 6 319 2 249 326 0.0 6 325 1 250 374 0.0 6 369 5 251 393 0.0 6 385 8 252 376 0.0 6 364 10 0 1 0 1 253 391 0.0 6 387 3 0 0 0 1 254 333 0.0 6 327 4 1 0 0 1 255 348 0.0 6 346 2 256 372 0.0 6 370 2 257 373 0.0 6 371 1 0 0 0 1 258 328 0.0 6 327 0 0 1 259 353 0.0 6 349 3 0 0 0 0 1 260 338 0.0 6 337 1 261 381 0.0 6 381 262 338 0.0 6 333 3 1 0 1 263 270 0.0 6 267 3 264 263 0.0 6 259 4 265 230 0.0 6 226 4 266 251 0.0 6 250 1 267 266 0.0 6 264 2 268 287 0.0 6 286 1 269 272 0.0 6 271 1 270 275 0.0 6 271 4 271 296 0.0 6 291 4 0 1 272 236 0.0 6 232 4 273 244 0.0 6 242 2 274 239 0.0 6 236 2 0 0 0 0 1 275 288 0.0 6 286 1 0 0 0 0 1 276 336 0.0 6 335 1 277 368 0.0 6 362 6 278 538 0.0 6 533 5 279 683 0.0 6 678 5 280 2282 0.0 6 2231 42 3 1 0 1 4 281 1931 0.0 6 1920 9 0 0 0 0 2 282 13 0.0 6 13 283 1 0.0 6 1 285 3 0.0 6 3 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1211 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 32 0.0 6 30 2 68 1 0.0 6 1 69 1 0.0 6 1 70 4 0.0 6 4 71 11 0.0 6 11 72 1 0.0 6 1 73 4 0.0 6 4 74 4 0.0 6 4 75 8 0.0 6 8 76 6 0.0 6 6 77 3 0.0 6 3 78 7 0.0 6 7 79 4 0.0 6 4 80 3 0.0 6 3 81 7 0.0 6 7 82 2 0.0 6 2 83 6 0.0 6 6 84 3 0.0 6 3 85 1 0.0 6 1 86 3 0.0 6 3 87 8 0.0 6 8 88 5 0.0 6 5 90 1 0.0 6 1 92 3 0.0 6 3 93 5 0.0 6 5 94 2 0.0 6 2 95 6 0.0 6 6 96 2 0.0 6 2 97 2 0.0 6 2 98 2 0.0 6 2 99 7 0.0 6 7 100 6 0.0 6 6 102 2 0.0 6 2 103 3 0.0 6 3 104 2 0.0 6 2 105 4 0.0 6 4 106 7 0.0 6 7 107 11 0.0 6 11 108 10 0.0 6 10 109 4 0.0 6 4 110 4 0.0 6 4 112 2 0.0 6 2 113 4 0.0 6 4 114 3 0.0 6 3 115 1 0.0 6 1 116 2 0.0 6 2 117 5 0.0 6 5 118 6 0.0 6 6 119 2 0.0 6 2 120 3 0.0 6 3 121 5 0.0 6 5 122 4 0.0 6 4 123 8 0.0 6 8 124 2 0.0 6 2 125 3 0.0 6 3 126 2 0.0 6 2 127 6 0.0 6 6 128 1 0.0 6 1 129 3 0.0 6 3 130 2 0.0 6 2 131 1 0.0 6 1 132 6 0.0 6 6 133 4 0.0 6 4 134 6 0.0 6 6 135 3 0.0 6 3 136 3 0.0 6 3 137 5 0.0 6 5 138 6 0.0 6 6 139 3 0.0 6 3 140 6 0.0 6 6 141 3 0.0 6 3 142 4 0.0 6 4 143 2 0.0 6 2 144 3 0.0 6 3 145 3 0.0 6 3 146 5 0.0 6 5 147 7 0.0 6 7 148 8 0.0 6 8 149 4 0.0 6 4 150 2 0.0 6 2 151 4 0.0 6 4 152 6 0.0 6 6 153 9 0.0 6 9 154 3 0.0 6 3 155 4 0.0 6 4 156 5 0.0 6 4 1 157 10 0.0 6 10 158 1 0.0 6 1 159 3 0.0 6 3 160 5 0.0 6 5 161 3 0.0 6 3 162 8 0.0 6 8 163 1 0.0 6 1 164 2 0.0 6 2 165 10 0.0 6 10 166 6 0.0 6 6 167 7 0.0 6 7 168 11 0.0 6 11 169 6 0.0 6 6 170 9 0.0 6 9 171 3 0.0 6 3 172 9 0.0 6 9 173 6 0.0 6 6 174 6 0.0 6 6 175 5 0.0 6 5 176 6 0.0 6 6 177 14 0.0 6 14 178 3 0.0 6 3 179 9 0.0 6 9 180 7 0.0 6 7 181 5 0.0 6 5 182 8 0.0 6 8 183 8 0.0 6 7 1 184 6 0.0 6 6 185 6 0.0 6 6 186 14 0.0 6 14 187 8 0.0 6 8 188 9 0.0 6 9 189 8 0.0 6 8 190 7 0.0 6 7 191 4 0.0 6 4 192 7 0.0 6 7 193 4 0.0 6 4 194 7 0.0 6 7 195 11 0.0 6 11 196 3 0.0 6 3 197 5 0.0 6 5 198 15 0.0 6 15 199 9 0.0 6 9 200 6 0.0 6 6 201 7 0.0 6 7 202 6 0.0 6 6 203 11 0.0 6 11 204 8 0.0 6 8 205 10 0.0 6 10 206 7 0.0 6 7 207 8 0.0 6 8 208 4 0.0 6 4 209 8 0.0 6 8 210 10 0.0 6 10 211 10 0.0 6 10 212 4 0.0 6 4 213 7 0.0 6 7 214 6 0.0 6 6 215 6 0.0 6 6 216 7 0.0 6 7 217 6 0.0 6 6 218 8 0.0 6 8 219 9 0.0 6 9 220 7 0.0 6 7 221 10 0.0 6 10 222 12 0.0 6 12 223 8 0.0 6 8 224 7 0.0 6 7 225 9 0.0 6 9 226 5 0.0 6 5 227 7 0.0 6 7 228 10 0.0 6 10 229 5 0.0 6 5 230 11 0.0 6 11 231 7 0.0 6 7 232 9 0.0 6 9 233 5 0.0 6 5 234 6 0.0 6 6 235 10 0.0 6 10 236 1 0.0 6 1 237 7 0.0 6 7 238 18 0.0 6 18 239 5 0.0 6 5 240 5 0.0 6 5 241 12 0.0 6 12 242 9 0.0 6 9 243 12 0.0 6 12 244 7 0.0 6 7 245 13 0.0 6 13 246 14 0.0 6 14 247 11 0.0 6 11 248 4 0.0 6 4 249 6 0.0 6 6 250 11 0.0 6 11 251 10 0.0 6 10 252 5 0.0 6 5 253 3 0.0 6 3 254 4 0.0 6 4 255 6 0.0 6 6 256 2 0.0 6 2 257 3 0.0 6 3 258 5 0.0 6 5 259 5 0.0 6 5 260 3 0.0 6 3 261 1 0.0 6 1 262 2 0.0 6 2 264 1 0.0 6 1 265 2 0.0 6 2 266 1 0.0 6 1 267 3 0.0 6 3 268 4 0.0 6 4 269 5 0.0 6 5 270 2 0.0 6 2 271 2 0.0 6 2 272 2 0.0 6 2 273 1 0.0 6 1 276 1 0.0 6 1 277 2 0.0 6 2 278 2 0.0 6 2 279 2 0.0 6 2 280 5 0.0 6 5 281 2 0.0 6 2 282 2 0.0 6 2 283 1 0.0 6 1 284 3 0.0 6 3 285 2 0.0 6 2 286 2 0.0 6 2 287 1 0.0 6 1 288 4 0.0 6 4 289 2 0.0 6 2 290 3 0.0 6 3 291 1 0.0 6 1 292 1 0.0 6 1 293 1 0.0 6 1 294 1 0.0 6 1 295 1 0.0 6 1 297 1 0.0 6 1 298 1 0.0 6 1 302 5 0.0 6 5 303 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 55039 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 217.7 1 0 1 9 4 0.2 2 4 11 3 0.0 3 3 12 2 0.0 3 2 13 19 0.0 3 18 1 14 7 0.0 4 6 1 15 1 0.0 4 1 16 7 0.0 4 5 2 17 23 0.0 5 22 1 18 10 0.0 5 7 2 1 19 24 0.0 5 23 1 20 122 0.0 6 117 5 21 62 0.0 6 61 1 22 45 0.0 6 43 2 23 26 0.0 6 24 2 24 28 0.0 6 26 2 25 20 0.0 6 18 2 26 25 0.0 6 22 3 27 19 0.0 6 17 2 28 28 0.0 6 28 29 12 0.0 6 12 30 8 0.0 6 8 31 19 0.0 6 18 0 0 0 0 0 1 32 19 0.0 6 19 33 9 0.0 6 8 1 34 13 0.0 6 12 1 35 16 0.0 6 15 1 36 8 0.0 6 7 1 37 15 0.0 6 14 1 38 13 0.0 6 12 1 39 14 0.0 6 10 2 1 0 1 40 10 0.0 6 8 1 0 1 41 20 0.0 6 20 42 13 0.0 6 10 2 0 0 0 1 43 15 0.0 6 15 44 14 0.0 6 14 45 15 0.0 6 15 46 16 0.0 6 14 2 47 22 0.0 6 20 1 0 1 48 19 0.0 6 18 1 49 14 0.0 6 12 2 50 21 0.0 6 21 51 18 0.0 6 18 52 20 0.0 6 16 4 53 23 0.0 6 21 2 54 35 0.0 6 32 2 1 55 24 0.0 6 23 1 56 23 0.0 6 22 1 57 19 0.0 6 19 58 21 0.0 6 21 59 28 0.0 6 24 3 1 60 37 0.0 6 35 2 61 22 0.0 6 22 62 26 0.0 6 24 2 63 24 0.0 6 23 1 64 29 0.0 6 28 1 65 33 0.0 6 31 1 0 1 66 36 0.0 6 34 1 0 0 1 67 34 0.0 6 32 1 0 1 68 53 0.0 6 51 2 69 116 0.0 6 111 4 1 70 45 0.0 6 45 71 52 0.0 6 51 1 72 37 0.0 6 32 4 0 1 73 50 0.0 6 48 2 74 37 0.0 6 34 3 75 37 0.0 6 35 2 76 46 0.0 6 43 2 0 0 0 0 1 77 36 0.0 6 35 1 78 46 0.0 6 45 1 79 46 0.0 6 43 1 1 0 0 0 1 80 48 0.0 6 45 3 81 63 0.0 6 58 4 0 0 0 0 1 82 70 0.0 6 66 3 0 0 0 1 83 57 0.0 6 56 0 0 0 0 0 1 84 52 0.0 6 51 1 85 73 0.0 6 69 3 1 86 49 0.0 6 47 2 87 43 0.0 6 40 3 88 56 0.0 6 56 89 52 0.0 6 49 3 90 58 0.0 6 55 3 91 70 0.0 6 67 3 92 61 0.0 6 58 2 0 1 93 76 0.0 6 74 2 94 66 0.0 6 63 2 1 95 73 0.0 6 70 3 96 73 0.0 6 72 1 97 98 0.0 6 96 2 98 102 0.0 6 99 2 0 0 1 99 73 0.0 6 69 3 0 0 0 1 100 90 0.0 6 87 2 1 101 80 0.0 6 78 2 102 99 0.0 6 88 9 0 1 0 0 1 103 87 0.0 6 82 4 0 0 1 104 91 0.0 6 89 2 105 113 0.0 6 106 6 0 1 106 181 0.0 6 173 7 0 0 0 1 107 112 0.0 6 109 3 108 124 0.0 6 121 1 0 1 0 0 1 109 123 0.0 6 122 1 110 142 0.0 6 138 3 0 1 111 103 0.0 6 101 1 1 112 136 0.0 6 131 5 113 146 0.0 6 142 4 114 164 0.0 6 160 4 115 161 0.0 6 159 1 1 116 185 0.0 6 174 10 1 117 178 0.0 6 172 5 1 118 191 0.0 6 186 5 119 233 0.0 6 222 9 1 0 0 1 120 180 0.0 6 173 7 121 173 0.0 6 166 5 2 122 189 0.0 6 183 5 1 123 183 0.0 6 178 3 0 1 1 124 203 0.0 6 193 9 0 0 1 125 204 0.0 6 191 10 1 0 1 1 126 243 0.0 6 235 8 127 305 0.0 6 295 9 1 128 289 0.0 6 276 10 0 1 1 0 1 129 319 0.0 6 300 16 0 1 1 1 130 355 0.0 6 345 9 0 0 0 1 131 347 0.0 6 346 0 0 0 0 0 1 132 342 0.0 6 325 14 0 0 0 2 1 133 406 0.0 6 395 11 134 432 0.0 6 419 11 2 135 461 0.0 6 447 12 1 0 1 136 522 0.0 6 505 17 137 481 0.0 6 470 11 138 493 0.0 6 482 9 1 0 0 1 139 585 0.0 6 573 11 0 1 140 554 0.0 6 539 12 1 0 1 1 141 592 0.0 6 574 16 1 1 142 615 0.0 6 602 12 0 1 143 740 0.0 6 715 17 3 2 1 0 2 144 710 0.0 6 686 18 1 1 1 2 1 145 664 0.0 6 647 15 0 1 1 146 780 0.0 6 766 10 1 0 2 1 147 745 0.0 6 719 19 3 0 0 0 4 148 804 0.0 6 778 21 1 3 1 149 787 0.0 6 767 18 0 1 0 1 150 918 0.0 6 892 16 2 1 2 4 1 151 1015 0.0 6 990 22 1 0 1 0 1 152 1406 0.0 6 1369 30 0 4 2 1 153 1036 0.0 6 995 32 5 0 2 2 154 1147 0.0 6 1119 25 2 0 1 155 1202 0.0 6 1174 24 2 0 0 0 2 156 852 0.0 6 832 18 2 157 988 0.0 6 959 25 1 0 1 1 1 158 882 0.0 6 868 9 1 2 1 0 1 159 959 0.0 6 924 23 0 4 1 4 3 160 955 0.0 6 933 18 2 2 161 835 0.0 6 813 20 1 0 0 0 1 162 784 0.0 6 763 18 0 0 1 2 163 762 0.0 6 743 15 2 0 0 1 1 164 655 0.0 6 643 10 1 0 1 165 726 0.0 6 710 13 1 1 1 166 635 0.0 6 622 11 1 0 0 0 1 167 646 0.0 6 637 7 1 0 0 1 168 753 0.0 6 740 11 0 1 0 1 169 582 0.0 6 570 8 1 1 1 0 1 170 747 0.0 6 731 14 0 0 0 1 1 171 570 0.0 6 556 12 1 0 0 1 172 626 0.0 6 615 9 2 173 714 0.0 6 698 14 1 0 0 0 1 174 758 0.0 6 741 14 1 1 0 1 175 687 0.0 6 671 13 1 1 1 176 649 0.0 6 638 9 0 0 1 1 177 603 0.0 6 588 13 0 1 0 0 1 178 618 0.0 6 607 9 1 0 0 0 1 179 561 0.0 6 549 11 0 0 0 0 1 180 484 0.0 6 475 6 2 0 0 0 1 181 409 0.0 6 400 9 182 373 0.0 6 369 2 0 1 0 1 183 384 0.0 6 379 4 0 0 0 0 1 184 343 0.0 6 338 4 1 185 572 0.0 6 555 12 0 2 1 1 1 186 690 0.0 6 684 6 187 587 0.0 6 578 8 1 188 447 0.0 6 439 6 1 0 0 1 189 408 0.0 6 399 7 0 0 2 190 387 0.0 6 374 11 1 0 1 191 386 0.0 6 380 2 1 0 1 2 192 305 0.0 6 303 1 0 1 193 309 0.0 6 303 6 194 260 0.0 6 255 5 195 308 0.0 6 304 4 196 291 0.0 6 286 5 197 224 0.0 6 220 2 1 1 198 361 0.0 6 357 3 1 199 329 0.0 6 316 11 0 1 0 0 1 200 241 0.0 6 231 9 0 0 1 201 250 0.0 6 248 1 1 202 189 0.0 6 184 5 203 152 0.0 6 148 3 1 204 180 0.0 6 177 2 0 0 0 0 1 205 141 0.0 6 140 1 206 129 0.0 6 127 2 207 98 0.0 6 97 0 0 0 0 1 208 129 0.0 6 127 2 209 98 0.0 6 97 1 210 128 0.0 6 125 3 211 108 0.0 6 104 4 212 77 0.0 6 77 213 78 0.0 6 77 1 214 70 0.0 6 69 1 215 84 0.0 6 82 2 216 111 0.0 6 108 3 217 85 0.0 6 82 2 0 0 0 0 1 218 60 0.0 6 59 1 219 67 0.0 6 65 2 220 57 0.0 6 56 0 0 0 1 221 80 0.0 6 80 222 40 0.0 6 39 1 223 53 0.0 6 53 224 60 0.0 6 57 2 0 0 0 0 1 225 50 0.0 6 49 1 226 52 0.0 6 51 1 227 32 0.0 6 32 228 24 0.0 6 24 229 31 0.0 6 31 230 32 0.0 6 32 231 23 0.0 6 23 232 29 0.0 6 29 233 28 0.0 6 26 2 234 24 0.0 6 24 235 11 0.0 6 11 236 9 0.0 6 8 1 237 10 0.0 6 10 238 3 0.0 6 3 239 3 0.0 6 3 240 13 0.0 6 13 241 9 0.0 6 9 242 10 0.0 6 10 243 3 0.0 6 3 244 7 0.0 6 7 245 6 0.0 6 6 246 4 0.0 6 4 247 6 0.0 6 6 248 5 0.0 6 5 249 4 0.0 6 4 250 3 0.0 6 3 251 3 0.0 6 3 253 3 0.0 6 3 279 3 0.0 6 3 280 9 0.0 6 8 1 281 87 0.0 6 85 2 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 650 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 51 2 0.0 6 2 53 3 0.0 6 3 54 5 0.0 6 5 55 1 0.0 6 1 67 1 0.0 6 1 69 1 0.0 6 1 70 11 0.0 6 11 71 10 0.0 6 10 72 12 0.0 6 12 73 6 0.0 6 6 74 3 0.0 6 3 75 5 0.0 6 5 76 10 0.0 6 10 77 4 0.0 6 4 78 8 0.0 6 8 79 1 0.0 6 1 80 3 0.0 6 3 81 1 0.0 6 1 82 5 0.0 6 5 83 5 0.0 6 5 84 3 0.0 6 3 85 2 0.0 6 2 86 3 0.0 6 3 87 4 0.0 6 4 88 2 0.0 6 2 89 1 0.0 6 1 90 2 0.0 6 2 91 2 0.0 6 2 92 6 0.0 6 6 93 2 0.0 6 2 94 3 0.0 6 3 95 3 0.0 6 3 96 3 0.0 6 3 97 3 0.0 6 3 98 5 0.0 6 5 99 2 0.0 6 2 100 2 0.0 6 2 101 2 0.0 6 2 102 1 0.0 6 1 103 4 0.0 6 4 104 3 0.0 6 3 105 5 0.0 6 5 106 1 0.0 6 1 107 4 0.0 6 4 108 10 0.0 6 10 109 3 0.0 6 3 110 2 0.0 6 2 111 4 0.0 6 4 112 1 0.0 6 1 113 4 0.0 6 4 114 3 0.0 6 2 1 115 1 0.0 6 1 116 3 0.0 6 3 117 1 0.0 6 1 118 3 0.0 6 3 119 1 0.0 6 1 120 5 0.0 6 5 121 3 0.0 6 3 122 2 0.0 6 2 123 4 0.0 6 4 124 2 0.0 6 2 125 6 0.0 6 6 126 3 0.0 6 3 127 7 0.0 6 7 128 3 0.0 6 3 129 2 0.0 6 2 130 2 0.0 6 2 131 6 0.0 6 6 132 8 0.0 6 8 133 5 0.0 6 5 134 2 0.0 6 2 135 4 0.0 6 4 136 7 0.0 6 7 137 2 0.0 6 2 138 5 0.0 6 5 139 9 0.0 6 9 140 1 0.0 6 1 141 4 0.0 6 4 142 2 0.0 6 2 143 2 0.0 6 2 144 4 0.0 6 4 145 1 0.0 6 1 146 5 0.0 6 5 147 7 0.0 6 7 148 2 0.0 6 2 149 6 0.0 6 6 150 3 0.0 6 3 151 5 0.0 6 4 0 0 1 152 5 0.0 6 5 153 7 0.0 6 7 154 5 0.0 6 5 155 5 0.0 6 5 156 13 0.0 6 13 157 8 0.0 6 8 158 5 0.0 6 5 159 5 0.0 6 5 160 2 0.0 6 2 161 7 0.0 6 7 162 3 0.0 6 3 163 6 0.0 6 6 164 4 0.0 6 4 165 6 0.0 6 6 166 9 0.0 6 9 167 2 0.0 6 2 168 19 0.0 6 19 169 5 0.0 6 5 170 12 0.0 6 11 1 171 7 0.0 6 7 172 7 0.0 6 7 173 3 0.0 6 3 174 2 0.0 6 2 175 5 0.0 6 5 176 3 0.0 6 3 177 7 0.0 6 6 0 0 0 1 178 7 0.0 6 7 179 6 0.0 6 5 0 1 180 5 0.0 6 5 181 1 0.0 6 1 182 2 0.0 6 2 183 4 0.0 6 4 184 3 0.0 6 3 185 6 0.0 6 6 186 15 0.0 6 15 187 9 0.0 6 9 188 8 0.0 6 8 189 5 0.0 6 5 190 9 0.0 6 9 191 4 0.0 6 4 192 8 0.0 6 8 193 1 0.0 6 1 194 7 0.0 6 7 195 3 0.0 6 3 196 3 0.0 6 3 198 6 0.0 6 6 199 2 0.0 6 2 200 4 0.0 6 4 201 2 0.0 6 2 202 3 0.0 6 3 203 1 0.0 6 1 204 6 0.0 6 6 205 1 0.0 6 1 206 2 0.0 6 2 207 2 0.0 6 2 208 1 0.0 6 1 209 2 0.0 6 2 210 2 0.0 6 2 212 1 0.0 6 1 213 1 0.0 6 1 214 1 0.0 6 1 217 1 0.0 6 1 219 3 0.0 6 3 220 2 0.0 6 2 221 2 0.0 6 2 224 1 0.0 6 1 229 1 0.0 6 1 231 1 0.0 6 1 235 1 0.0 6 1 246 1 0.0 6 1 247 1 0.0 6 1 250 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_02_37_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_02_264_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_02_37_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_02_264_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.17 s (1125 µs/read; 0.05 M reads/minute). === Summary === Total read pairs processed: 154 Read 1 with adapter: 153 (99.4%) Read 2 with adapter: 154 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 1 (0.6%) Pairs written (passing filters): 153 (99.4%) Total basepairs processed: 65,420 bp Read 1: 37,685 bp Read 2: 27,735 bp Total written (filtered): 9,402 bp (14.4%) Read 1: 4,662 bp Read 2: 4,740 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 152 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 21 1 0.0 6 1 68 1 0.0 6 1 91 1 0.0 6 1 107 1 0.0 6 1 120 1 0.0 6 1 126 1 0.0 6 1 145 2 0.0 6 2 151 1 0.0 6 1 152 1 0.0 6 1 154 2 0.0 6 2 158 1 0.0 6 1 164 2 0.0 6 2 166 1 0.0 6 1 168 1 0.0 6 1 169 1 0.0 6 1 180 1 0.0 6 1 181 4 0.0 6 4 183 3 0.0 6 3 184 1 0.0 6 1 185 1 0.0 6 1 187 1 0.0 6 1 188 2 0.0 6 2 189 1 0.0 6 1 194 2 0.0 6 2 195 1 0.0 6 1 198 1 0.0 6 1 199 2 0.0 6 1 1 200 1 0.0 6 1 201 3 0.0 6 2 1 202 1 0.0 6 1 203 3 0.0 6 3 204 3 0.0 6 3 205 1 0.0 6 1 207 1 0.0 6 1 208 4 0.0 6 4 209 1 0.0 6 1 211 3 0.0 6 3 214 4 0.0 6 4 215 2 0.0 6 2 216 3 0.0 6 3 217 1 0.0 6 1 218 2 0.0 6 2 219 3 0.0 6 3 220 4 0.0 6 4 221 1 0.0 6 1 222 2 0.0 6 2 224 3 0.0 6 3 225 1 0.0 6 1 226 1 0.0 6 1 227 3 0.0 6 3 228 2 0.0 6 2 230 1 0.0 6 1 231 5 0.0 6 5 232 2 0.0 6 2 234 5 0.0 6 5 235 3 0.0 6 3 236 1 0.0 6 1 237 1 0.0 6 1 238 4 0.0 6 4 239 1 0.0 6 1 240 3 0.0 6 3 243 3 0.0 6 3 244 3 0.0 6 3 245 2 0.0 6 2 248 1 0.0 6 1 252 3 0.0 6 3 253 1 0.0 6 1 254 2 0.0 6 2 260 1 0.0 6 1 262 2 0.0 6 2 264 1 0.0 6 1 265 1 0.0 6 1 266 2 0.0 6 2 268 1 0.0 6 1 269 1 0.0 6 1 270 2 0.0 6 0 1 0 1 278 2 0.0 6 2 279 1 0.0 6 1 280 1 0.0 6 1 281 5 0.0 6 5 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 190 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 153 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 17 1 0.0 5 1 20 1 0.0 6 1 21 1 0.0 6 1 28 1 0.0 6 1 43 1 0.0 6 1 51 1 0.0 6 1 63 1 0.0 6 1 65 2 0.0 6 2 67 1 0.0 6 0 1 68 1 0.0 6 1 78 1 0.0 6 1 94 1 0.0 6 1 96 1 0.0 6 1 97 1 0.0 6 1 99 1 0.0 6 1 100 1 0.0 6 1 104 1 0.0 6 1 105 1 0.0 6 1 109 1 0.0 6 0 1 111 1 0.0 6 1 114 1 0.0 6 1 115 1 0.0 6 1 118 1 0.0 6 1 119 1 0.0 6 1 120 1 0.0 6 1 124 1 0.0 6 1 126 2 0.0 6 2 127 1 0.0 6 0 1 129 1 0.0 6 1 130 1 0.0 6 1 131 1 0.0 6 1 133 1 0.0 6 1 134 1 0.0 6 1 135 2 0.0 6 2 136 1 0.0 6 1 138 2 0.0 6 2 140 3 0.0 6 3 141 3 0.0 6 3 143 2 0.0 6 2 144 1 0.0 6 1 145 4 0.0 6 4 146 4 0.0 6 4 147 3 0.0 6 3 148 1 0.0 6 1 149 3 0.0 6 3 150 4 0.0 6 3 1 151 10 0.0 6 10 152 4 0.0 6 4 153 1 0.0 6 1 154 2 0.0 6 2 155 3 0.0 6 3 156 2 0.0 6 2 158 4 0.0 6 4 159 1 0.0 6 1 160 4 0.0 6 4 161 2 0.0 6 2 162 3 0.0 6 3 164 1 0.0 6 1 165 2 0.0 6 2 167 3 0.0 6 2 1 168 4 0.0 6 4 169 2 0.0 6 2 170 1 0.0 6 1 172 1 0.0 6 1 173 2 0.0 6 2 174 2 0.0 6 2 175 1 0.0 6 1 176 3 0.0 6 3 178 1 0.0 6 1 179 2 0.0 6 2 180 1 0.0 6 1 184 1 0.0 6 1 185 1 0.0 6 1 186 2 0.0 6 2 187 1 0.0 6 1 188 1 0.0 6 1 190 2 0.0 6 2 192 1 0.0 6 1 195 1 0.0 6 1 196 1 0.0 6 1 197 2 0.0 6 2 206 1 0.0 6 1 211 2 0.0 6 2 212 2 0.0 6 2 215 1 0.0 6 1 219 1 0.0 6 1 228 1 0.0 6 1 280 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 160 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_03_38_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_03_265_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_03_38_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_03_265_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.75 s (24 µs/read; 2.49 M reads/minute). === Summary === Total read pairs processed: 72,421 Read 1 with adapter: 72,415 (100.0%) Read 2 with adapter: 72,354 (99.9%) == Read fate breakdown == Pairs that were too short: 43 (0.1%) Pairs discarded as untrimmed: 73 (0.1%) Pairs written (passing filters): 72,305 (99.8%) Total basepairs processed: 31,230,601 bp Read 1: 17,509,673 bp Read 2: 13,720,928 bp Total written (filtered): 4,628,002 bp (14.8%) Read 1: 2,262,739 bp Read 2: 2,365,263 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 70483 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 1131.6 0 2 1 4 2 282.9 1 1 1 6 1 17.7 1 0 0 1 7 2 4.4 2 2 8 2 1.1 2 1 1 9 3 0.3 2 1 2 10 5 0.1 3 4 1 15 1 0.0 4 1 16 2 0.0 4 1 1 17 3 0.0 5 3 19 6 0.0 5 4 2 20 141 0.0 6 141 21 92 0.0 6 91 1 22 76 0.0 6 73 2 1 23 36 0.0 6 35 1 24 30 0.0 6 30 25 28 0.0 6 27 1 26 23 0.0 6 23 27 13 0.0 6 13 28 15 0.0 6 14 0 0 0 0 1 29 16 0.0 6 15 1 30 10 0.0 6 10 31 4 0.0 6 4 32 11 0.0 6 10 1 33 9 0.0 6 9 34 11 0.0 6 10 0 1 35 6 0.0 6 6 36 9 0.0 6 9 37 10 0.0 6 10 38 4 0.0 6 3 1 39 4 0.0 6 4 40 7 0.0 6 6 1 41 7 0.0 6 6 1 42 8 0.0 6 8 43 7 0.0 6 7 44 9 0.0 6 9 45 6 0.0 6 6 46 8 0.0 6 7 1 47 5 0.0 6 3 2 48 10 0.0 6 10 49 7 0.0 6 6 1 50 9 0.0 6 8 1 51 3 0.0 6 3 52 9 0.0 6 9 53 9 0.0 6 9 54 5 0.0 6 5 55 8 0.0 6 7 1 56 5 0.0 6 4 1 57 3 0.0 6 3 58 10 0.0 6 10 59 5 0.0 6 5 60 3 0.0 6 3 61 5 0.0 6 4 1 62 2 0.0 6 1 0 0 1 63 5 0.0 6 5 64 2 0.0 6 2 65 7 0.0 6 7 66 8 0.0 6 7 1 67 6 0.0 6 5 0 0 0 0 0 1 68 8 0.0 6 8 69 12 0.0 6 12 70 8 0.0 6 8 71 7 0.0 6 7 72 13 0.0 6 13 73 8 0.0 6 8 74 13 0.0 6 9 2 0 0 0 1 1 75 8 0.0 6 6 2 76 9 0.0 6 9 77 15 0.0 6 14 1 78 7 0.0 6 7 79 13 0.0 6 13 80 7 0.0 6 7 81 5 0.0 6 4 1 82 5 0.0 6 5 83 11 0.0 6 11 84 11 0.0 6 11 85 16 0.0 6 16 86 12 0.0 6 12 87 11 0.0 6 10 0 1 88 6 0.0 6 6 89 10 0.0 6 9 1 90 7 0.0 6 7 91 7 0.0 6 7 92 19 0.0 6 19 93 9 0.0 6 9 94 7 0.0 6 7 95 8 0.0 6 6 2 96 7 0.0 6 7 97 15 0.0 6 14 1 98 5 0.0 6 5 99 10 0.0 6 10 100 15 0.0 6 14 1 101 11 0.0 6 11 102 15 0.0 6 14 1 103 19 0.0 6 18 0 1 104 20 0.0 6 19 1 105 23 0.0 6 21 1 1 106 21 0.0 6 20 1 107 21 0.0 6 21 108 17 0.0 6 14 1 0 0 0 0 2 109 13 0.0 6 13 110 22 0.0 6 21 1 111 20 0.0 6 20 112 18 0.0 6 18 113 21 0.0 6 20 0 0 0 0 0 1 114 27 0.0 6 27 115 24 0.0 6 24 116 27 0.0 6 27 117 28 0.0 6 24 3 1 118 23 0.0 6 22 1 119 25 0.0 6 25 120 27 0.0 6 24 1 0 0 0 2 121 29 0.0 6 27 0 0 0 0 2 122 24 0.0 6 22 1 0 0 0 1 123 19 0.0 6 19 124 26 0.0 6 25 1 125 22 0.0 6 22 126 27 0.0 6 27 127 31 0.0 6 30 1 128 26 0.0 6 26 129 22 0.0 6 22 130 29 0.0 6 29 131 29 0.0 6 28 1 132 43 0.0 6 42 1 133 39 0.0 6 39 134 28 0.0 6 28 135 40 0.0 6 40 136 43 0.0 6 40 2 1 137 111 0.0 6 108 3 138 53 0.0 6 52 1 139 43 0.0 6 42 1 140 34 0.0 6 34 141 41 0.0 6 41 142 67 0.0 6 66 1 143 182 0.0 6 181 1 144 327 0.0 6 325 2 145 362 0.0 6 361 1 146 282 0.0 6 282 147 316 0.0 6 313 3 148 610 0.0 6 608 1 0 0 0 1 149 419 0.0 6 419 150 442 0.0 6 442 151 396 0.0 6 394 2 152 416 0.0 6 413 2 0 0 0 1 153 528 0.0 6 528 154 344 0.0 6 341 3 155 276 0.0 6 273 1 2 156 258 0.0 6 256 2 157 228 0.0 6 228 158 242 0.0 6 240 0 0 0 1 1 159 215 0.0 6 214 1 160 212 0.0 6 211 1 161 362 0.0 6 361 1 162 345 0.0 6 345 163 269 0.0 6 267 2 164 329 0.0 6 328 1 165 259 0.0 6 259 166 281 0.0 6 281 167 232 0.0 6 232 168 230 0.0 6 229 1 169 275 0.0 6 274 1 170 283 0.0 6 282 1 171 291 0.0 6 290 1 172 248 0.0 6 247 1 173 287 0.0 6 286 1 174 293 0.0 6 292 1 175 313 0.0 6 311 2 176 262 0.0 6 260 2 177 261 0.0 6 260 0 0 0 0 0 1 178 367 0.0 6 366 1 179 254 0.0 6 253 1 180 258 0.0 6 258 181 409 0.0 6 407 2 182 382 0.0 6 379 3 183 295 0.0 6 295 184 332 0.0 6 328 4 185 367 0.0 6 365 1 1 186 363 0.0 6 362 1 187 487 0.0 6 486 1 188 388 0.0 6 387 1 189 438 0.0 6 438 190 483 0.0 6 483 191 383 0.0 6 381 2 192 403 0.0 6 401 1 1 193 491 0.0 6 488 3 194 435 0.0 6 434 1 195 476 0.0 6 471 5 196 541 0.0 6 537 2 0 0 0 0 2 197 552 0.0 6 551 1 198 752 0.0 6 745 4 2 0 0 0 1 199 1020 0.0 6 1014 5 1 200 827 0.0 6 821 4 1 0 0 1 201 686 0.0 6 683 3 202 686 0.0 6 682 2 0 0 1 0 1 203 636 0.0 6 633 2 0 0 0 0 1 204 650 0.0 6 646 2 1 0 0 1 205 779 0.0 6 777 1 0 0 0 0 1 206 848 0.0 6 845 2 0 0 0 1 207 675 0.0 6 673 1 1 208 948 0.0 6 940 7 1 209 711 0.0 6 706 3 0 2 210 806 0.0 6 799 7 211 661 0.0 6 655 5 0 0 1 212 669 0.0 6 663 6 213 763 0.0 6 760 3 214 920 0.0 6 916 2 1 0 0 0 1 215 770 0.0 6 762 7 0 0 0 1 216 853 0.0 6 848 5 217 1050 0.0 6 1043 6 0 0 0 1 218 882 0.0 6 875 6 0 0 0 0 1 219 855 0.0 6 847 8 220 866 0.0 6 863 1 0 1 0 1 221 856 0.0 6 853 3 222 668 0.0 6 663 2 0 1 1 1 223 660 0.0 6 655 5 224 696 0.0 6 688 6 0 1 0 1 225 733 0.0 6 730 3 226 805 0.0 6 799 4 0 0 1 1 227 854 0.0 6 849 4 0 0 0 1 228 816 0.0 6 810 6 229 752 0.0 6 750 2 230 643 0.0 6 641 2 231 665 0.0 6 660 4 0 1 232 719 0.0 6 714 5 233 696 0.0 6 692 4 234 709 0.0 6 704 5 235 718 0.0 6 711 5 0 1 1 236 647 0.0 6 642 4 0 0 0 0 1 237 621 0.0 6 614 7 238 639 0.0 6 635 3 0 0 0 1 239 606 0.0 6 600 2 1 0 1 2 240 611 0.0 6 609 2 241 623 0.0 6 620 2 1 242 560 0.0 6 556 4 243 554 0.0 6 546 7 0 1 244 579 0.0 6 576 3 245 589 0.0 6 586 3 246 507 0.0 6 507 247 427 0.0 6 425 1 0 0 0 1 248 401 0.0 6 399 1 0 1 249 354 0.0 6 352 1 0 1 250 419 0.0 6 416 2 1 251 369 0.0 6 368 1 252 413 0.0 6 411 1 0 0 0 0 1 253 344 0.0 6 343 1 254 308 0.0 6 306 2 255 323 0.0 6 323 256 341 0.0 6 338 3 257 315 0.0 6 315 258 306 0.0 6 304 1 1 259 318 0.0 6 314 4 260 302 0.0 6 301 1 261 322 0.0 6 321 1 262 269 0.0 6 268 1 263 219 0.0 6 219 264 245 0.0 6 245 265 254 0.0 6 254 266 238 0.0 6 234 3 0 0 0 1 267 220 0.0 6 218 2 268 214 0.0 6 214 269 233 0.0 6 233 270 205 0.0 6 205 271 250 0.0 6 248 2 272 206 0.0 6 206 273 199 0.0 6 197 2 274 183 0.0 6 181 2 275 211 0.0 6 208 3 276 266 0.0 6 265 1 277 322 0.0 6 320 2 278 331 0.0 6 327 1 2 1 279 477 0.0 6 473 3 0 1 280 1461 0.0 6 1450 10 0 0 0 1 281 2062 0.0 6 2051 10 0 1 282 7 0.0 6 7 284 1 0.0 6 1 285 1 0.0 6 1 286 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1932 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 67 0.0 6 66 1 62 2 0.0 6 2 66 1 0.0 6 1 69 5 0.0 6 5 70 5 0.0 6 5 71 7 0.0 6 7 72 10 0.0 6 10 73 9 0.0 6 9 74 10 0.0 6 10 75 8 0.0 6 8 76 6 0.0 6 6 77 16 0.0 6 16 78 14 0.0 6 14 79 11 0.0 6 11 80 17 0.0 6 17 81 12 0.0 6 12 82 13 0.0 6 13 83 5 0.0 6 5 84 7 0.0 6 7 85 4 0.0 6 4 86 4 0.0 6 4 87 3 0.0 6 3 88 17 0.0 6 17 89 5 0.0 6 5 90 7 0.0 6 7 91 11 0.0 6 11 92 7 0.0 6 6 1 93 9 0.0 6 9 94 2 0.0 6 2 95 5 0.0 6 5 96 8 0.0 6 8 97 6 0.0 6 6 98 10 0.0 6 10 99 4 0.0 6 4 100 7 0.0 6 7 101 8 0.0 6 8 102 11 0.0 6 11 103 5 0.0 6 5 104 6 0.0 6 5 1 105 5 0.0 6 5 106 4 0.0 6 4 107 7 0.0 6 7 108 18 0.0 6 18 109 10 0.0 6 10 110 7 0.0 6 7 111 6 0.0 6 6 112 15 0.0 6 15 113 10 0.0 6 10 114 10 0.0 6 10 115 4 0.0 6 4 116 9 0.0 6 9 117 11 0.0 6 11 118 8 0.0 6 8 119 6 0.0 6 6 120 11 0.0 6 11 121 7 0.0 6 7 122 9 0.0 6 9 123 7 0.0 6 7 124 7 0.0 6 7 125 16 0.0 6 16 126 19 0.0 6 19 127 5 0.0 6 5 128 7 0.0 6 7 129 17 0.0 6 16 0 0 0 0 0 1 130 5 0.0 6 5 131 9 0.0 6 9 132 21 0.0 6 21 133 22 0.0 6 22 134 5 0.0 6 5 135 10 0.0 6 10 136 9 0.0 6 9 137 10 0.0 6 10 138 9 0.0 6 9 139 9 0.0 6 9 140 11 0.0 6 11 141 11 0.0 6 11 142 9 0.0 6 9 143 6 0.0 6 6 144 8 0.0 6 8 145 13 0.0 6 13 146 8 0.0 6 8 147 10 0.0 6 10 148 9 0.0 6 9 149 13 0.0 6 13 150 13 0.0 6 13 151 9 0.0 6 9 152 8 0.0 6 8 153 12 0.0 6 12 154 11 0.0 6 11 155 12 0.0 6 12 156 5 0.0 6 5 157 5 0.0 6 5 158 11 0.0 6 11 159 9 0.0 6 9 160 10 0.0 6 10 161 12 0.0 6 12 162 17 0.0 6 17 163 11 0.0 6 11 164 13 0.0 6 13 165 25 0.0 6 24 1 166 14 0.0 6 14 167 11 0.0 6 11 168 10 0.0 6 10 169 10 0.0 6 10 170 15 0.0 6 15 171 8 0.0 6 8 172 14 0.0 6 14 173 8 0.0 6 8 174 10 0.0 6 10 175 8 0.0 6 8 176 14 0.0 6 14 177 18 0.0 6 18 178 11 0.0 6 11 179 8 0.0 6 8 180 11 0.0 6 11 181 9 0.0 6 9 182 11 0.0 6 11 183 11 0.0 6 11 184 8 0.0 6 8 185 17 0.0 6 17 186 15 0.0 6 15 187 17 0.0 6 17 188 8 0.0 6 8 189 13 0.0 6 13 190 9 0.0 6 9 191 10 0.0 6 10 192 14 0.0 6 14 193 12 0.0 6 12 194 11 0.0 6 11 195 8 0.0 6 8 196 13 0.0 6 12 1 197 15 0.0 6 15 198 15 0.0 6 15 199 11 0.0 6 11 200 11 0.0 6 11 201 20 0.0 6 20 202 13 0.0 6 13 203 12 0.0 6 12 204 16 0.0 6 16 205 10 0.0 6 10 206 12 0.0 6 12 207 9 0.0 6 9 208 11 0.0 6 11 209 14 0.0 6 14 210 13 0.0 6 13 211 14 0.0 6 14 212 11 0.0 6 11 213 6 0.0 6 6 214 6 0.0 6 6 215 5 0.0 6 5 216 15 0.0 6 15 217 12 0.0 6 12 218 10 0.0 6 10 219 5 0.0 6 5 220 8 0.0 6 8 221 7 0.0 6 7 222 10 0.0 6 10 223 6 0.0 6 6 224 8 0.0 6 8 225 10 0.0 6 10 226 6 0.0 6 6 227 11 0.0 6 11 228 12 0.0 6 12 229 13 0.0 6 13 230 4 0.0 6 4 231 2 0.0 6 2 232 10 0.0 6 10 233 7 0.0 6 7 234 12 0.0 6 11 0 0 0 0 0 1 235 7 0.0 6 7 236 7 0.0 6 7 237 8 0.0 6 8 238 4 0.0 6 4 239 4 0.0 6 4 240 10 0.0 6 10 241 7 0.0 6 7 242 5 0.0 6 5 243 4 0.0 6 4 244 4 0.0 6 4 245 3 0.0 6 3 246 5 0.0 6 5 247 6 0.0 6 6 248 7 0.0 6 7 249 4 0.0 6 4 250 6 0.0 6 6 251 6 0.0 6 6 252 4 0.0 6 4 253 3 0.0 6 3 254 2 0.0 6 2 255 4 0.0 6 4 256 1 0.0 6 1 257 2 0.0 6 2 258 3 0.0 6 3 259 6 0.0 6 6 260 1 0.0 6 1 261 7 0.0 6 7 262 3 0.0 6 3 263 4 0.0 6 4 264 4 0.0 6 4 265 2 0.0 6 2 266 2 0.0 6 2 267 2 0.0 6 2 268 3 0.0 6 3 269 2 0.0 6 2 270 2 0.0 6 2 271 3 0.0 6 3 273 2 0.0 6 2 274 2 0.0 6 2 275 3 0.0 6 3 276 2 0.0 6 2 277 3 0.0 6 3 278 3 0.0 6 3 279 2 0.0 6 2 280 2 0.0 6 2 281 2 0.0 6 2 282 4 0.0 6 4 283 2 0.0 6 2 285 2 0.0 6 2 289 1 0.0 6 1 290 2 0.0 6 2 292 1 0.0 6 1 297 1 0.0 6 1 302 1 0.0 6 1 304 1 0.0 6 1 309 1 0.0 6 1 311 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 70779 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1131.6 0 1 1 4 1 282.9 1 1 6 1 17.7 1 1 7 2 4.4 2 2 9 2 0.3 2 2 10 7 0.1 3 7 11 4 0.0 3 3 1 12 5 0.0 3 4 1 13 9 0.0 3 9 14 11 0.0 4 10 1 16 9 0.0 4 6 3 17 27 0.0 5 27 18 24 0.0 5 22 2 19 22 0.0 5 19 3 20 139 0.0 6 131 8 21 55 0.0 6 55 22 31 0.0 6 27 4 23 30 0.0 6 29 0 0 1 24 26 0.0 6 24 1 0 1 25 28 0.0 6 27 1 26 29 0.0 6 28 1 27 11 0.0 6 10 1 28 22 0.0 6 22 29 25 0.0 6 21 3 1 30 26 0.0 6 25 1 31 15 0.0 6 13 2 32 10 0.0 6 10 33 18 0.0 6 15 2 1 34 23 0.0 6 21 1 0 0 0 1 35 16 0.0 6 14 2 36 14 0.0 6 14 37 12 0.0 6 12 38 15 0.0 6 14 0 0 0 0 0 1 39 17 0.0 6 17 40 10 0.0 6 10 41 19 0.0 6 16 2 1 42 13 0.0 6 10 3 43 18 0.0 6 17 1 44 16 0.0 6 15 1 45 17 0.0 6 16 1 46 27 0.0 6 25 1 1 47 24 0.0 6 24 48 22 0.0 6 20 2 49 22 0.0 6 19 2 0 1 50 19 0.0 6 17 1 0 0 0 0 1 51 25 0.0 6 25 52 19 0.0 6 15 3 1 53 28 0.0 6 25 3 54 27 0.0 6 27 55 21 0.0 6 21 56 31 0.0 6 29 1 1 57 20 0.0 6 19 1 58 15 0.0 6 15 59 31 0.0 6 31 60 38 0.0 6 37 1 61 25 0.0 6 23 2 62 36 0.0 6 33 2 1 63 45 0.0 6 43 2 64 46 0.0 6 42 4 65 36 0.0 6 34 2 66 41 0.0 6 38 2 0 1 67 146 0.0 6 136 9 1 68 52 0.0 6 50 2 69 75 0.0 6 69 4 0 1 1 70 38 0.0 6 35 3 71 71 0.0 6 69 1 0 0 1 72 37 0.0 6 33 4 73 40 0.0 6 37 3 74 29 0.0 6 26 2 0 1 75 41 0.0 6 41 76 35 0.0 6 30 4 0 0 1 77 43 0.0 6 40 3 78 48 0.0 6 47 1 79 43 0.0 6 42 0 0 0 1 80 70 0.0 6 66 2 1 0 1 81 61 0.0 6 58 3 82 59 0.0 6 55 4 83 73 0.0 6 67 4 2 84 43 0.0 6 39 3 0 0 1 85 44 0.0 6 44 86 37 0.0 6 37 87 50 0.0 6 43 5 1 1 88 49 0.0 6 46 2 1 89 51 0.0 6 47 4 90 72 0.0 6 70 1 1 91 71 0.0 6 69 2 92 55 0.0 6 53 1 0 0 0 1 93 92 0.0 6 85 5 2 94 70 0.0 6 67 3 95 85 0.0 6 81 4 96 107 0.0 6 99 7 0 1 97 60 0.0 6 57 3 98 67 0.0 6 64 2 0 0 1 99 75 0.0 6 74 1 100 84 0.0 6 82 1 0 0 1 101 74 0.0 6 70 3 0 0 1 102 81 0.0 6 77 4 103 100 0.0 6 99 1 104 181 0.0 6 172 8 1 105 102 0.0 6 96 5 1 106 97 0.0 6 90 7 107 104 0.0 6 100 3 1 108 95 0.0 6 92 3 109 109 0.0 6 107 2 110 125 0.0 6 123 2 111 144 0.0 6 137 5 1 0 0 0 1 112 145 0.0 6 136 6 2 0 1 113 149 0.0 6 139 9 0 1 114 146 0.0 6 144 2 115 182 0.0 6 179 3 116 165 0.0 6 153 11 1 117 146 0.0 6 140 4 0 0 1 0 1 118 197 0.0 6 191 6 119 155 0.0 6 151 4 120 153 0.0 6 149 3 1 121 202 0.0 6 196 5 0 0 0 1 122 173 0.0 6 168 3 0 0 1 1 123 204 0.0 6 197 6 0 0 1 124 246 0.0 6 235 10 1 125 251 0.0 6 242 8 0 0 1 126 256 0.0 6 250 4 0 1 0 1 127 289 0.0 6 281 6 1 0 0 1 128 348 0.0 6 331 14 1 0 1 0 1 129 372 0.0 6 362 10 130 361 0.0 6 351 9 1 131 370 0.0 6 364 5 1 132 367 0.0 6 354 13 133 451 0.0 6 440 9 1 0 0 1 134 465 0.0 6 458 7 135 439 0.0 6 435 4 136 444 0.0 6 438 5 0 0 0 0 1 137 479 0.0 6 469 8 0 0 0 1 1 138 585 0.0 6 575 10 139 689 0.0 6 671 14 1 1 1 1 140 766 0.0 6 751 8 1 0 0 2 4 141 809 0.0 6 795 12 0 0 0 2 142 902 0.0 6 885 13 0 2 1 1 143 986 0.0 6 976 8 0 0 1 0 1 144 965 0.0 6 949 14 1 0 0 0 1 145 979 0.0 6 968 8 1 0 1 1 146 1092 0.0 6 1080 11 1 147 1150 0.0 6 1129 19 1 1 148 1311 0.0 6 1293 15 0 2 0 0 1 149 1164 0.0 6 1155 7 0 0 0 1 1 150 1464 0.0 6 1433 25 2 0 2 0 2 151 1152 0.0 6 1129 20 2 0 0 1 152 1390 0.0 6 1361 24 1 0 0 3 1 153 1370 0.0 6 1354 11 1 2 1 0 1 154 1154 0.0 6 1138 14 1 0 0 1 155 1167 0.0 6 1148 17 0 1 0 1 156 1073 0.0 6 1052 16 1 0 3 1 157 1269 0.0 6 1241 22 1 1 2 0 2 158 1275 0.0 6 1252 21 0 1 0 1 159 1078 0.0 6 1053 22 2 1 160 1143 0.0 6 1118 22 0 1 1 0 1 161 1005 0.0 6 986 12 1 1 3 1 1 162 942 0.0 6 922 17 1 0 1 1 163 953 0.0 6 941 11 0 0 0 0 1 164 923 0.0 6 915 4 2 1 0 0 1 165 912 0.0 6 898 9 0 3 0 0 2 166 1116 0.0 6 1100 15 1 167 932 0.0 6 925 7 168 1069 0.0 6 1059 5 1 1 1 1 1 169 1073 0.0 6 1056 13 2 0 1 0 1 170 996 0.0 6 978 12 1 1 1 2 1 171 1171 0.0 6 1160 11 172 1073 0.0 6 1059 10 1 0 1 0 2 173 1066 0.0 6 1052 11 1 0 0 1 1 174 1008 0.0 6 997 10 1 175 958 0.0 6 948 10 176 1152 0.0 6 1141 10 0 0 0 0 1 177 865 0.0 6 856 7 0 1 0 0 1 178 842 0.0 6 837 3 1 0 0 0 1 179 730 0.0 6 720 7 0 3 180 591 0.0 6 583 8 181 648 0.0 6 638 10 182 663 0.0 6 653 10 183 844 0.0 6 834 6 3 1 184 1108 0.0 6 1095 12 0 0 1 185 955 0.0 6 947 6 0 0 0 2 186 690 0.0 6 685 5 187 672 0.0 6 666 5 1 188 675 0.0 6 673 2 189 618 0.0 6 611 6 0 0 1 190 542 0.0 6 534 6 1 1 191 592 0.0 6 583 8 1 192 448 0.0 6 442 4 0 1 0 0 1 193 402 0.0 6 398 3 1 194 379 0.0 6 374 3 1 0 0 0 1 195 326 0.0 6 322 4 196 456 0.0 6 454 2 197 385 0.0 6 379 4 0 0 1 0 1 198 338 0.0 6 334 4 199 289 0.0 6 287 2 200 299 0.0 6 298 1 201 229 0.0 6 226 2 1 202 226 0.0 6 221 3 0 0 0 1 1 203 222 0.0 6 218 4 204 210 0.0 6 205 4 1 205 218 0.0 6 215 2 0 1 206 188 0.0 6 187 0 0 0 0 0 1 207 187 0.0 6 186 1 208 148 0.0 6 148 209 146 0.0 6 145 0 1 210 119 0.0 6 118 1 211 94 0.0 6 94 212 118 0.0 6 117 1 213 98 0.0 6 98 214 138 0.0 6 135 3 215 102 0.0 6 102 216 103 0.0 6 101 2 217 89 0.0 6 87 0 1 0 0 0 1 218 68 0.0 6 67 1 219 53 0.0 6 53 220 37 0.0 6 36 1 221 76 0.0 6 75 1 222 47 0.0 6 46 1 223 52 0.0 6 52 224 41 0.0 6 41 225 25 0.0 6 25 226 25 0.0 6 25 227 17 0.0 6 17 228 19 0.0 6 19 229 19 0.0 6 19 230 11 0.0 6 11 231 14 0.0 6 14 232 15 0.0 6 15 233 8 0.0 6 8 234 5 0.0 6 5 235 6 0.0 6 6 236 8 0.0 6 8 237 1 0.0 6 1 238 7 0.0 6 7 239 6 0.0 6 6 240 3 0.0 6 3 241 2 0.0 6 2 242 6 0.0 6 6 243 3 0.0 6 3 245 2 0.0 6 2 246 4 0.0 6 4 247 1 0.0 6 1 248 3 0.0 6 3 249 1 0.0 6 1 275 1 0.0 6 0 1 277 3 0.0 6 3 278 12 0.0 6 10 2 279 84 0.0 6 83 0 0 1 280 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1575 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 49 5 0.0 6 0 5 60 3 0.0 6 3 65 5 0.0 6 3 2 66 106 0.0 6 104 2 67 3 0.0 6 2 1 69 5 0.0 6 5 70 2 0.0 6 1 0 0 0 1 71 8 0.0 6 8 72 9 0.0 6 9 73 9 0.0 6 9 74 14 0.0 6 14 75 18 0.0 6 18 76 19 0.0 6 19 77 39 0.0 6 39 78 33 0.0 6 33 79 25 0.0 6 25 80 13 0.0 6 13 81 12 0.0 6 12 82 34 0.0 6 34 83 14 0.0 6 14 84 3 0.0 6 3 85 10 0.0 6 10 86 7 0.0 6 7 87 12 0.0 6 11 0 0 0 1 88 6 0.0 6 6 89 8 0.0 6 8 90 13 0.0 6 13 91 17 0.0 6 17 92 8 0.0 6 8 93 10 0.0 6 10 94 9 0.0 6 9 95 6 0.0 6 6 96 8 0.0 6 8 97 5 0.0 6 5 98 8 0.0 6 8 99 2 0.0 6 2 100 8 0.0 6 8 101 6 0.0 6 6 102 5 0.0 6 5 103 7 0.0 6 7 104 8 0.0 6 8 105 8 0.0 6 8 106 8 0.0 6 8 107 15 0.0 6 14 0 0 0 1 108 23 0.0 6 23 109 7 0.0 6 7 110 17 0.0 6 17 111 15 0.0 6 15 112 15 0.0 6 15 113 7 0.0 6 7 114 5 0.0 6 5 115 7 0.0 6 7 116 14 0.0 6 14 117 5 0.0 6 5 118 8 0.0 6 8 119 15 0.0 6 15 120 6 0.0 6 6 121 7 0.0 6 7 122 8 0.0 6 8 123 2 0.0 6 2 124 4 0.0 6 4 125 9 0.0 6 8 1 126 6 0.0 6 6 127 11 0.0 6 11 128 20 0.0 6 19 0 0 0 1 129 7 0.0 6 7 130 5 0.0 6 5 131 12 0.0 6 12 132 7 0.0 6 7 133 12 0.0 6 12 134 13 0.0 6 13 135 22 0.0 6 22 136 8 0.0 6 7 1 137 11 0.0 6 11 138 15 0.0 6 14 0 0 0 1 139 14 0.0 6 14 140 11 0.0 6 11 141 17 0.0 6 17 142 9 0.0 6 9 143 13 0.0 6 13 144 6 0.0 6 6 145 12 0.0 6 12 146 9 0.0 6 9 147 24 0.0 6 23 0 0 0 1 148 7 0.0 6 7 149 15 0.0 6 14 0 0 0 1 150 9 0.0 6 9 151 5 0.0 6 5 152 11 0.0 6 11 153 14 0.0 6 13 0 0 0 1 154 6 0.0 6 6 155 8 0.0 6 8 156 10 0.0 6 10 157 15 0.0 6 15 158 17 0.0 6 17 159 11 0.0 6 11 160 9 0.0 6 9 161 10 0.0 6 10 162 11 0.0 6 11 163 18 0.0 6 18 164 27 0.0 6 27 165 6 0.0 6 6 166 10 0.0 6 10 167 17 0.0 6 17 168 13 0.0 6 13 169 11 0.0 6 11 170 23 0.0 6 23 171 5 0.0 6 5 172 10 0.0 6 10 173 7 0.0 6 7 174 11 0.0 6 11 175 8 0.0 6 8 176 7 0.0 6 7 177 8 0.0 6 8 178 11 0.0 6 11 179 5 0.0 6 5 180 11 0.0 6 11 181 6 0.0 6 6 182 12 0.0 6 12 183 4 0.0 6 4 184 5 0.0 6 5 185 6 0.0 6 6 186 9 0.0 6 9 187 8 0.0 6 8 188 7 0.0 6 7 189 10 0.0 6 10 190 5 0.0 6 5 191 6 0.0 6 6 192 3 0.0 6 3 193 4 0.0 6 4 194 3 0.0 6 3 195 7 0.0 6 7 196 4 0.0 6 4 197 4 0.0 6 4 198 3 0.0 6 3 199 1 0.0 6 1 200 7 0.0 6 7 201 3 0.0 6 3 202 3 0.0 6 3 203 8 0.0 6 8 204 2 0.0 6 2 205 3 0.0 6 3 206 6 0.0 6 6 207 8 0.0 6 8 208 3 0.0 6 3 209 8 0.0 6 8 210 4 0.0 6 4 211 1 0.0 6 1 212 2 0.0 6 2 213 4 0.0 6 4 214 3 0.0 6 3 215 2 0.0 6 2 216 3 0.0 6 3 217 1 0.0 6 1 218 3 0.0 6 3 219 1 0.0 6 1 220 1 0.0 6 1 221 1 0.0 6 1 222 3 0.0 6 3 223 2 0.0 6 2 224 1 0.0 6 1 228 1 0.0 6 1 234 1 0.0 6 1 237 2 0.0 6 2 238 1 0.0 6 1 246 2 0.0 6 2 259 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_04_39_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_04_266_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_04_39_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_04_266_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.14 s (39 µs/read; 1.53 M reads/minute). === Summary === Total read pairs processed: 29,241 Read 1 with adapter: 29,236 (100.0%) Read 2 with adapter: 29,223 (99.9%) == Read fate breakdown == Pairs that were too short: 25 (0.1%) Pairs discarded as untrimmed: 19 (0.1%) Pairs written (passing filters): 29,197 (99.8%) Total basepairs processed: 12,502,061 bp Read 1: 7,321,288 bp Read 2: 5,180,773 bp Total written (filtered): 1,745,186 bp (14.0%) Read 1: 896,715 bp Read 2: 848,471 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 28570 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 456.9 0 1 1 4 2 114.2 1 1 1 6 3 7.1 1 0 0 3 8 1 0.4 2 1 10 2 0.0 3 2 15 2 0.0 4 2 17 1 0.0 5 1 20 23 0.0 6 22 1 21 10 0.0 6 9 0 0 0 0 1 22 24 0.0 6 24 23 12 0.0 6 12 24 5 0.0 6 5 25 7 0.0 6 7 26 6 0.0 6 6 27 3 0.0 6 3 28 4 0.0 6 4 29 1 0.0 6 1 30 1 0.0 6 1 31 2 0.0 6 2 32 2 0.0 6 2 33 2 0.0 6 2 34 3 0.0 6 3 35 4 0.0 6 4 36 3 0.0 6 3 37 3 0.0 6 3 39 3 0.0 6 3 40 4 0.0 6 4 42 1 0.0 6 1 43 2 0.0 6 2 44 9 0.0 6 9 45 2 0.0 6 2 46 2 0.0 6 2 47 10 0.0 6 10 48 5 0.0 6 5 49 2 0.0 6 2 50 3 0.0 6 3 51 2 0.0 6 2 52 2 0.0 6 2 54 7 0.0 6 7 55 6 0.0 6 4 2 56 2 0.0 6 2 57 2 0.0 6 1 1 58 3 0.0 6 3 59 3 0.0 6 3 61 5 0.0 6 5 63 1 0.0 6 1 64 1 0.0 6 1 66 9 0.0 6 9 67 2 0.0 6 2 68 2 0.0 6 2 69 4 0.0 6 4 70 4 0.0 6 3 1 71 3 0.0 6 2 1 72 6 0.0 6 4 2 73 5 0.0 6 3 2 74 7 0.0 6 6 1 76 3 0.0 6 2 1 78 5 0.0 6 4 1 79 4 0.0 6 4 80 1 0.0 6 1 81 3 0.0 6 3 83 5 0.0 6 5 84 2 0.0 6 2 85 1 0.0 6 1 86 3 0.0 6 3 87 7 0.0 6 6 1 88 3 0.0 6 2 0 1 89 5 0.0 6 5 90 2 0.0 6 2 91 5 0.0 6 5 92 1 0.0 6 1 93 1 0.0 6 1 94 3 0.0 6 3 95 3 0.0 6 3 96 4 0.0 6 4 97 2 0.0 6 2 98 5 0.0 6 4 1 99 2 0.0 6 2 100 10 0.0 6 10 101 2 0.0 6 2 102 6 0.0 6 6 103 2 0.0 6 2 104 3 0.0 6 3 105 3 0.0 6 3 106 4 0.0 6 4 107 3 0.0 6 3 108 3 0.0 6 3 109 9 0.0 6 8 1 110 2 0.0 6 2 111 9 0.0 6 9 112 11 0.0 6 11 113 7 0.0 6 6 1 114 6 0.0 6 6 115 7 0.0 6 7 116 13 0.0 6 12 1 117 10 0.0 6 9 1 118 9 0.0 6 7 0 0 1 0 1 119 6 0.0 6 6 120 9 0.0 6 7 1 1 121 5 0.0 6 4 1 122 2 0.0 6 2 123 8 0.0 6 7 1 124 9 0.0 6 9 125 5 0.0 6 5 126 6 0.0 6 6 127 11 0.0 6 11 128 6 0.0 6 6 129 4 0.0 6 4 130 15 0.0 6 15 131 6 0.0 6 6 132 11 0.0 6 11 133 13 0.0 6 10 3 134 10 0.0 6 10 135 5 0.0 6 5 136 18 0.0 6 18 137 16 0.0 6 16 138 14 0.0 6 14 139 16 0.0 6 16 140 17 0.0 6 17 141 20 0.0 6 19 1 142 48 0.0 6 45 2 0 0 0 0 1 143 13 0.0 6 13 144 28 0.0 6 27 1 145 26 0.0 6 26 146 29 0.0 6 28 1 147 12 0.0 6 12 148 21 0.0 6 21 149 20 0.0 6 18 2 150 29 0.0 6 28 1 151 18 0.0 6 17 1 152 16 0.0 6 15 0 0 0 0 0 1 153 21 0.0 6 20 1 154 30 0.0 6 29 1 155 22 0.0 6 21 1 156 26 0.0 6 25 0 1 157 20 0.0 6 18 2 158 43 0.0 6 42 1 159 27 0.0 6 26 1 160 19 0.0 6 18 1 161 23 0.0 6 22 0 0 1 162 33 0.0 6 32 1 163 37 0.0 6 37 164 41 0.0 6 39 2 165 48 0.0 6 42 5 1 166 66 0.0 6 65 1 167 45 0.0 6 43 2 168 30 0.0 6 29 1 169 61 0.0 6 58 2 0 0 0 1 170 67 0.0 6 64 3 171 109 0.0 6 105 3 0 0 0 1 172 45 0.0 6 39 6 173 70 0.0 6 67 1 0 1 0 1 174 64 0.0 6 63 1 175 46 0.0 6 45 0 0 1 176 76 0.0 6 75 1 177 118 0.0 6 116 1 0 1 178 478 0.0 6 476 2 179 123 0.0 6 121 2 180 59 0.0 6 57 2 181 78 0.0 6 75 3 182 97 0.0 6 96 1 183 89 0.0 6 82 4 2 0 0 1 184 116 0.0 6 113 3 185 119 0.0 6 116 3 186 105 0.0 6 104 1 187 111 0.0 6 106 3 1 0 0 0 1 188 158 0.0 6 156 2 189 207 0.0 6 201 6 190 68 0.0 6 63 4 0 0 0 0 1 191 96 0.0 6 93 2 0 1 192 107 0.0 6 103 4 193 229 0.0 6 225 4 194 101 0.0 6 97 3 1 195 241 0.0 6 236 4 0 1 196 213 0.0 6 207 4 1 0 1 197 245 0.0 6 240 5 198 390 0.0 6 377 9 2 1 0 1 199 633 0.0 6 624 9 200 303 0.0 6 292 7 1 0 0 0 3 201 396 0.0 6 388 7 0 0 0 1 202 561 0.0 6 549 8 3 0 0 1 203 302 0.0 6 296 4 2 204 197 0.0 6 193 2 0 2 205 254 0.0 6 248 6 206 238 0.0 6 226 11 1 207 471 0.0 6 457 12 0 1 0 1 208 800 0.0 6 784 14 0 1 0 0 1 209 623 0.0 6 612 7 0 0 0 2 2 210 264 0.0 6 253 8 0 0 1 2 211 398 0.0 6 377 18 0 0 1 1 1 212 183 0.0 6 176 7 213 336 0.0 6 324 10 0 2 214 381 0.0 6 370 11 215 285 0.0 6 270 11 0 0 0 1 3 216 500 0.0 6 483 13 1 0 0 3 217 460 0.0 6 447 10 0 0 0 1 2 218 316 0.0 6 299 16 1 219 374 0.0 6 364 9 1 220 377 0.0 6 371 5 0 0 0 0 1 221 336 0.0 6 330 5 1 222 257 0.0 6 250 6 1 223 318 0.0 6 310 6 0 1 0 0 1 224 310 0.0 6 305 5 225 504 0.0 6 492 9 0 0 0 2 1 226 227 0.0 6 218 7 1 1 227 269 0.0 6 260 7 1 0 0 1 228 337 0.0 6 324 11 0 1 0 0 1 229 233 0.0 6 225 7 1 230 318 0.0 6 313 4 0 0 0 1 231 333 0.0 6 320 12 0 0 0 1 232 474 0.0 6 463 9 1 1 233 338 0.0 6 331 6 0 1 234 513 0.0 6 496 13 1 0 0 2 1 235 628 0.0 6 612 15 0 0 0 1 236 313 0.0 6 307 5 1 237 464 0.0 6 451 12 0 0 0 1 238 185 0.0 6 182 2 0 0 0 1 239 357 0.0 6 352 5 240 449 0.0 6 443 6 241 404 0.0 6 402 2 242 600 0.0 6 595 3 1 0 1 243 266 0.0 6 259 4 3 244 208 0.0 6 206 2 245 282 0.0 6 279 3 246 355 0.0 6 351 4 247 379 0.0 6 378 1 248 71 0.0 6 69 2 249 127 0.0 6 124 3 250 66 0.0 6 64 1 1 251 132 0.0 6 127 4 0 1 252 94 0.0 6 94 253 113 0.0 6 112 1 254 129 0.0 6 128 0 0 1 255 146 0.0 6 143 1 1 0 0 0 1 256 98 0.0 6 98 257 180 0.0 6 177 1 1 0 0 1 258 85 0.0 6 84 0 0 0 0 1 259 103 0.0 6 103 260 40 0.0 6 40 261 96 0.0 6 95 0 1 262 54 0.0 6 53 1 263 134 0.0 6 133 1 264 22 0.0 6 22 265 40 0.0 6 40 266 27 0.0 6 27 267 66 0.0 6 66 268 33 0.0 6 33 269 103 0.0 6 102 0 0 0 1 270 80 0.0 6 80 271 40 0.0 6 40 272 76 0.0 6 76 273 30 0.0 6 30 274 33 0.0 6 32 1 275 73 0.0 6 73 276 49 0.0 6 49 277 78 0.0 6 77 0 1 278 150 0.0 6 150 279 275 0.0 6 273 2 280 1159 0.0 6 1154 5 281 709 0.0 6 706 3 282 4 0.0 6 4 284 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 666 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 96 1 0.0 6 1 97 1 0.0 6 1 106 1 0.0 6 1 107 2 0.0 6 2 108 26 0.0 6 26 109 3 0.0 6 3 161 8 0.0 6 8 162 26 0.0 6 26 163 4 0.0 6 4 164 21 0.0 6 21 165 75 0.0 6 75 166 4 0.0 6 3 1 169 1 0.0 6 1 170 1 0.0 6 1 171 28 0.0 6 28 172 111 0.0 6 111 173 3 0.0 6 2 1 186 1 0.0 6 1 187 5 0.0 6 5 188 25 0.0 6 25 205 1 0.0 6 1 208 6 0.0 6 6 209 37 0.0 6 37 210 120 0.0 6 120 211 7 0.0 6 7 213 5 0.0 6 5 214 18 0.0 6 18 215 1 0.0 6 1 266 1 0.0 6 1 267 3 0.0 6 3 268 3 0.0 6 2 1 269 19 0.0 6 19 270 54 0.0 6 54 271 1 0.0 6 1 275 1 0.0 6 1 276 4 0.0 6 4 281 9 0.0 6 9 282 28 0.0 6 28 293 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 28954 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.8% G: 0.1% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 1.8 2 1 11 2 0.0 3 0 2 12 3 0.0 3 3 13 2 0.0 3 2 14 5 0.0 4 3 1 0 1 15 1 0.0 4 1 16 3 0.0 4 3 17 14 0.0 5 12 2 18 1 0.0 5 0 1 19 40 0.0 5 35 4 1 20 47 0.0 6 46 1 21 34 0.0 6 31 3 22 16 0.0 6 14 2 23 24 0.0 6 23 1 24 8 0.0 6 8 25 15 0.0 6 14 0 0 0 1 26 13 0.0 6 12 1 27 5 0.0 6 5 28 10 0.0 6 9 1 29 5 0.0 6 5 30 6 0.0 6 5 0 1 31 4 0.0 6 4 32 12 0.0 6 9 2 1 33 12 0.0 6 7 5 34 8 0.0 6 8 35 2 0.0 6 2 36 10 0.0 6 10 37 9 0.0 6 9 38 14 0.0 6 14 39 2 0.0 6 2 40 14 0.0 6 12 2 41 8 0.0 6 6 2 42 9 0.0 6 7 2 43 8 0.0 6 7 1 44 6 0.0 6 6 45 6 0.0 6 6 46 3 0.0 6 3 47 10 0.0 6 10 48 11 0.0 6 10 1 49 25 0.0 6 25 50 12 0.0 6 12 51 24 0.0 6 20 3 0 0 1 52 13 0.0 6 10 2 0 1 53 17 0.0 6 16 1 54 10 0.0 6 8 1 0 0 1 55 10 0.0 6 8 1 1 56 12 0.0 6 11 1 57 10 0.0 6 9 0 0 0 1 58 11 0.0 6 9 2 59 17 0.0 6 15 1 0 0 0 0 1 60 17 0.0 6 17 61 13 0.0 6 11 2 62 18 0.0 6 16 2 63 8 0.0 6 8 64 14 0.0 6 11 3 65 21 0.0 6 20 1 66 16 0.0 6 16 67 12 0.0 6 11 0 0 0 0 0 1 68 15 0.0 6 15 69 30 0.0 6 29 0 0 0 0 0 1 70 65 0.0 6 63 2 71 45 0.0 6 43 1 0 0 0 1 72 36 0.0 6 33 3 73 27 0.0 6 23 4 74 33 0.0 6 30 3 75 22 0.0 6 21 1 76 32 0.0 6 30 1 1 77 21 0.0 6 21 78 34 0.0 6 31 2 0 0 1 79 25 0.0 6 22 1 1 0 0 0 1 80 40 0.0 6 36 4 81 27 0.0 6 25 2 82 37 0.0 6 36 1 83 50 0.0 6 46 4 84 30 0.0 6 29 1 85 45 0.0 6 41 4 86 43 0.0 6 41 2 87 22 0.0 6 20 1 0 0 1 88 21 0.0 6 20 1 89 25 0.0 6 24 1 90 30 0.0 6 27 2 0 0 1 91 31 0.0 6 30 1 92 37 0.0 6 37 93 16 0.0 6 13 2 1 94 49 0.0 6 47 2 95 47 0.0 6 46 1 96 47 0.0 6 43 4 97 33 0.0 6 29 3 0 0 0 0 1 98 56 0.0 6 55 1 99 66 0.0 6 60 4 0 0 1 0 1 100 37 0.0 6 36 0 0 0 1 101 65 0.0 6 61 4 102 50 0.0 6 46 4 103 35 0.0 6 35 104 89 0.0 6 88 1 105 50 0.0 6 45 5 106 120 0.0 6 118 2 107 116 0.0 6 106 8 1 1 108 115 0.0 6 105 10 109 96 0.0 6 91 5 110 85 0.0 6 78 7 111 67 0.0 6 62 5 112 69 0.0 6 68 1 113 76 0.0 6 74 2 114 80 0.0 6 78 2 115 176 0.0 6 164 10 0 1 1 116 240 0.0 6 227 12 1 117 103 0.0 6 100 3 118 241 0.0 6 232 9 119 157 0.0 6 151 5 0 0 1 120 104 0.0 6 100 4 121 303 0.0 6 292 10 1 122 131 0.0 6 126 5 123 89 0.0 6 85 4 124 172 0.0 6 161 8 2 0 0 0 1 125 160 0.0 6 153 6 0 0 0 0 1 126 167 0.0 6 162 5 127 261 0.0 6 248 12 1 128 186 0.0 6 179 6 0 0 1 129 206 0.0 6 200 6 130 402 0.0 6 389 10 1 0 1 0 1 131 173 0.0 6 166 6 1 132 255 0.0 6 248 5 0 0 1 0 1 133 348 0.0 6 332 15 1 134 515 0.0 6 501 14 135 175 0.0 6 171 4 136 412 0.0 6 396 16 137 485 0.0 6 461 21 2 0 1 138 284 0.0 6 270 10 2 0 1 1 139 296 0.0 6 284 11 1 140 362 0.0 6 352 9 0 0 0 0 1 141 227 0.0 6 219 7 1 142 391 0.0 6 383 7 1 143 429 0.0 6 421 8 144 293 0.0 6 286 6 0 1 145 358 0.0 6 342 15 0 0 0 0 1 146 328 0.0 6 314 13 0 0 0 0 1 147 264 0.0 6 256 6 0 0 1 0 1 148 468 0.0 6 453 15 149 376 0.0 6 361 14 0 0 0 0 1 150 312 0.0 6 302 7 2 0 0 0 1 151 572 0.0 6 551 19 1 0 0 0 1 152 796 0.0 6 771 25 153 712 0.0 6 675 33 2 0 0 0 2 154 618 0.0 6 596 19 0 1 1 0 1 155 642 0.0 6 622 17 1 0 0 0 2 156 811 0.0 6 780 25 2 0 2 0 2 157 542 0.0 6 516 24 1 0 0 1 158 886 0.0 6 850 34 0 0 0 0 2 159 449 0.0 6 436 13 160 548 0.0 6 535 10 0 0 1 0 2 161 459 0.0 6 453 6 162 441 0.0 6 428 13 163 439 0.0 6 425 13 1 164 336 0.0 6 325 10 1 165 233 0.0 6 226 7 166 310 0.0 6 305 5 167 232 0.0 6 223 9 168 318 0.0 6 309 9 169 406 0.0 6 396 10 170 348 0.0 6 340 8 171 325 0.0 6 313 11 0 0 0 0 1 172 310 0.0 6 306 4 173 258 0.0 6 248 9 0 0 0 0 1 174 402 0.0 6 391 8 2 0 0 1 175 443 0.0 6 431 12 176 415 0.0 6 407 8 177 232 0.0 6 226 6 178 287 0.0 6 283 3 0 0 0 0 1 179 340 0.0 6 329 11 180 273 0.0 6 267 4 0 1 0 0 1 181 259 0.0 6 255 2 0 0 1 0 1 182 160 0.0 6 159 1 183 123 0.0 6 122 1 184 89 0.0 6 88 1 185 151 0.0 6 148 3 186 242 0.0 6 237 5 187 251 0.0 6 247 2 1 0 0 0 1 188 257 0.0 6 256 1 189 150 0.0 6 149 1 190 80 0.0 6 76 4 191 77 0.0 6 76 1 192 48 0.0 6 44 4 193 51 0.0 6 49 2 194 53 0.0 6 51 2 195 44 0.0 6 43 1 196 79 0.0 6 79 197 57 0.0 6 56 1 198 44 0.0 6 42 2 199 61 0.0 6 59 2 200 54 0.0 6 54 201 29 0.0 6 29 202 33 0.0 6 33 203 30 0.0 6 29 1 204 25 0.0 6 24 1 205 26 0.0 6 26 206 20 0.0 6 20 207 19 0.0 6 19 208 9 0.0 6 9 209 11 0.0 6 8 2 1 210 11 0.0 6 11 211 18 0.0 6 18 212 8 0.0 6 8 213 23 0.0 6 22 0 0 0 0 0 1 214 7 0.0 6 7 215 4 0.0 6 4 216 6 0.0 6 6 217 16 0.0 6 16 218 14 0.0 6 14 219 9 0.0 6 8 0 0 0 0 0 1 220 6 0.0 6 6 221 5 0.0 6 5 222 6 0.0 6 6 223 5 0.0 6 5 224 4 0.0 6 4 225 9 0.0 6 9 226 5 0.0 6 5 227 9 0.0 6 9 228 9 0.0 6 9 229 8 0.0 6 8 230 2 0.0 6 2 231 1 0.0 6 1 232 4 0.0 6 4 233 3 0.0 6 3 234 1 0.0 6 1 235 1 0.0 6 1 236 1 0.0 6 1 244 1 0.0 6 1 245 1 0.0 6 1 250 1 0.0 6 1 252 1 0.0 6 0 1 280 2 0.0 6 2 281 4 0.0 6 4 282 15 0.0 6 15 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 269 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 1 0.0 6 0 1 52 1 0.0 6 1 71 1 0.0 6 1 95 3 0.0 6 3 100 1 0.0 6 1 102 1 0.0 6 1 105 1 0.0 6 1 106 4 0.0 6 4 107 25 0.0 6 24 1 160 3 0.0 6 3 161 35 0.0 6 35 162 1 0.0 6 1 163 10 0.0 6 10 164 58 0.0 6 58 169 2 0.0 6 2 170 10 0.0 6 10 171 81 0.0 6 81 172 1 0.0 6 1 186 2 0.0 6 2 187 10 0.0 6 10 208 3 0.0 6 3 209 12 0.0 6 12 210 1 0.0 6 1 212 1 0.0 6 0 0 0 0 1 213 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_05_40_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_05_267_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_05_40_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_05_267_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.03 s (26 µs/read; 2.27 M reads/minute). === Summary === Total read pairs processed: 76,864 Read 1 with adapter: 76,858 (100.0%) Read 2 with adapter: 76,821 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 48 (0.1%) Pairs written (passing filters): 76,816 (99.9%) Total basepairs processed: 38,987,228 bp Read 1: 22,274,038 bp Read 2: 16,713,190 bp Total written (filtered): 4,608,279 bp (11.8%) Read 1: 2,304,424 bp Read 2: 2,303,855 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 76844 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1201.0 0 0 1 4 3 300.2 1 1 2 6 2 18.8 1 0 0 2 7 1 4.7 2 1 9 1 0.3 2 1 11 1 0.0 3 1 15 1 0.0 4 1 16 3 0.0 4 1 2 17 1 0.0 5 1 20 13 0.0 6 13 21 7 0.0 6 6 1 22 2 0.0 6 1 1 23 2 0.0 6 2 24 3 0.0 6 3 25 15 0.0 6 15 26 6 0.0 6 5 1 27 3 0.0 6 3 28 3 0.0 6 2 1 29 2 0.0 6 2 30 2 0.0 6 2 31 2 0.0 6 2 32 1 0.0 6 0 1 33 1 0.0 6 1 34 2 0.0 6 2 35 2 0.0 6 2 37 1 0.0 6 1 39 3 0.0 6 3 40 2 0.0 6 1 1 41 2 0.0 6 2 42 1 0.0 6 0 1 43 2 0.0 6 1 0 1 44 5 0.0 6 5 45 7 0.0 6 7 46 3 0.0 6 3 48 2 0.0 6 2 49 1 0.0 6 1 52 1 0.0 6 1 53 1 0.0 6 1 54 1 0.0 6 1 55 1 0.0 6 1 56 2 0.0 6 1 1 57 3 0.0 6 2 1 58 5 0.0 6 5 60 3 0.0 6 3 61 1 0.0 6 1 62 3 0.0 6 3 63 2 0.0 6 2 64 2 0.0 6 2 66 1 0.0 6 0 1 67 1 0.0 6 1 68 1 0.0 6 1 69 1 0.0 6 0 1 71 1 0.0 6 0 1 72 2 0.0 6 2 73 1 0.0 6 1 75 1 0.0 6 1 77 2 0.0 6 2 78 5 0.0 6 5 79 1 0.0 6 1 80 1 0.0 6 0 1 81 2 0.0 6 2 82 3 0.0 6 3 84 1 0.0 6 1 85 1 0.0 6 1 86 12 0.0 6 11 1 87 4 0.0 6 4 88 1 0.0 6 0 1 89 4 0.0 6 4 90 3 0.0 6 3 91 1 0.0 6 1 92 2 0.0 6 2 93 2 0.0 6 2 94 2 0.0 6 2 95 2 0.0 6 2 96 1 0.0 6 1 97 6 0.0 6 6 98 7 0.0 6 5 2 100 3 0.0 6 2 1 101 5 0.0 6 5 102 28 0.0 6 27 1 103 8 0.0 6 7 1 104 7 0.0 6 6 1 105 4 0.0 6 3 1 106 3 0.0 6 2 1 107 19 0.0 6 19 108 11 0.0 6 11 109 6 0.0 6 6 110 1 0.0 6 1 111 11 0.0 6 11 112 6 0.0 6 4 1 1 113 11 0.0 6 10 1 114 20 0.0 6 20 115 8 0.0 6 8 116 9 0.0 6 8 1 117 7 0.0 6 6 0 1 118 6 0.0 6 5 0 1 119 7 0.0 6 7 120 5 0.0 6 5 121 10 0.0 6 8 2 122 16 0.0 6 16 123 18 0.0 6 18 124 7 0.0 6 5 2 125 7 0.0 6 7 126 12 0.0 6 9 3 127 9 0.0 6 9 128 18 0.0 6 16 2 129 7 0.0 6 6 1 130 4 0.0 6 4 131 13 0.0 6 13 132 11 0.0 6 11 133 7 0.0 6 7 134 13 0.0 6 13 135 18 0.0 6 17 1 136 13 0.0 6 12 1 137 25 0.0 6 25 138 16 0.0 6 14 2 139 5 0.0 6 5 140 14 0.0 6 14 141 13 0.0 6 13 142 20 0.0 6 20 143 29 0.0 6 27 2 144 29 0.0 6 28 1 145 26 0.0 6 24 2 146 18 0.0 6 17 1 147 20 0.0 6 19 0 0 1 148 24 0.0 6 24 149 19 0.0 6 18 1 150 11 0.0 6 10 1 151 25 0.0 6 23 1 1 152 16 0.0 6 16 153 13 0.0 6 13 154 22 0.0 6 22 155 25 0.0 6 23 1 0 0 1 156 14 0.0 6 14 157 17 0.0 6 17 158 20 0.0 6 19 1 159 14 0.0 6 14 160 21 0.0 6 21 161 19 0.0 6 19 162 19 0.0 6 19 163 34 0.0 6 33 1 164 27 0.0 6 27 165 44 0.0 6 44 166 28 0.0 6 28 167 25 0.0 6 25 168 18 0.0 6 16 2 169 28 0.0 6 28 170 53 0.0 6 51 2 171 47 0.0 6 41 6 172 46 0.0 6 42 4 173 45 0.0 6 43 2 174 53 0.0 6 51 2 175 62 0.0 6 61 1 176 46 0.0 6 44 2 177 45 0.0 6 45 178 53 0.0 6 51 2 179 46 0.0 6 45 1 180 47 0.0 6 44 2 1 181 56 0.0 6 55 1 182 55 0.0 6 52 3 183 60 0.0 6 58 2 184 71 0.0 6 68 3 185 87 0.0 6 84 3 186 112 0.0 6 110 2 187 92 0.0 6 88 4 188 101 0.0 6 99 2 189 104 0.0 6 100 4 190 111 0.0 6 109 2 191 156 0.0 6 154 2 192 159 0.0 6 156 3 193 137 0.0 6 135 1 1 194 142 0.0 6 138 4 195 146 0.0 6 142 4 196 160 0.0 6 156 4 197 141 0.0 6 137 4 198 178 0.0 6 175 3 199 186 0.0 6 184 2 200 216 0.0 6 212 4 201 149 0.0 6 144 4 0 1 202 188 0.0 6 183 5 203 190 0.0 6 186 4 204 227 0.0 6 217 8 2 205 259 0.0 6 257 2 206 186 0.0 6 181 3 2 207 205 0.0 6 199 6 208 251 0.0 6 246 5 209 206 0.0 6 204 2 210 177 0.0 6 172 5 211 212 0.0 6 209 3 212 183 0.0 6 175 7 1 213 217 0.0 6 207 9 1 214 336 0.0 6 328 8 215 182 0.0 6 178 2 1 1 216 314 0.0 6 305 9 217 298 0.0 6 289 9 218 193 0.0 6 189 3 1 219 234 0.0 6 226 8 220 440 0.0 6 431 9 221 259 0.0 6 249 9 1 222 307 0.0 6 301 5 1 223 444 0.0 6 429 14 0 1 224 281 0.0 6 269 12 225 493 0.0 6 485 8 226 375 0.0 6 368 6 0 1 227 345 0.0 6 340 4 1 228 434 0.0 6 423 10 0 1 229 275 0.0 6 267 8 230 565 0.0 6 551 12 1 1 231 268 0.0 6 258 9 0 0 1 232 321 0.0 6 313 7 1 233 274 0.0 6 268 6 234 286 0.0 6 283 3 235 256 0.0 6 245 11 236 291 0.0 6 282 9 237 270 0.0 6 262 8 238 367 0.0 6 357 10 239 349 0.0 6 339 10 240 505 0.0 6 493 12 241 414 0.0 6 409 4 1 242 357 0.0 6 350 7 243 347 0.0 6 337 9 1 244 429 0.0 6 422 7 245 455 0.0 6 444 11 246 462 0.0 6 455 7 247 565 0.0 6 554 11 248 366 0.0 6 359 6 0 1 249 449 0.0 6 445 4 250 550 0.0 6 541 7 2 251 624 0.0 6 603 20 1 252 585 0.0 6 571 14 253 459 0.0 6 447 12 254 355 0.0 6 346 9 255 689 0.0 6 663 26 256 813 0.0 6 797 16 257 731 0.0 6 718 13 258 550 0.0 6 534 16 259 911 0.0 6 896 15 260 694 0.0 6 678 16 261 786 0.0 6 765 21 262 488 0.0 6 470 18 263 614 0.0 6 598 15 1 264 540 0.0 6 525 13 1 1 265 504 0.0 6 496 7 1 266 525 0.0 6 506 18 1 267 698 0.0 6 678 19 1 268 666 0.0 6 638 27 1 269 920 0.0 6 898 22 270 490 0.0 6 474 15 1 271 995 0.0 6 967 27 1 272 730 0.0 6 711 19 273 593 0.0 6 585 8 274 612 0.0 6 599 13 275 695 0.0 6 672 23 276 1222 0.0 6 1198 22 2 277 1810 0.0 6 1769 40 1 278 2620 0.0 6 2559 58 1 1 1 279 4017 0.0 6 3925 87 5 280 10140 0.0 6 9913 217 7 2 1 281 20387 0.0 6 19997 376 9 2 2 1 282 68 0.0 6 57 9 2 283 7 0.0 6 5 0 2 284 4 0.0 6 3 0 0 1 285 7 0.0 6 4 0 1 2 286 3 0.0 6 2 1 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 14 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 91 1 0.0 6 1 100 1 0.0 6 1 103 1 0.0 6 1 112 1 0.0 6 1 139 1 0.0 6 1 171 1 0.0 6 1 249 8 0.0 6 8 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 76810 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 2 4.7 2 2 9 2 0.3 2 2 11 3 0.0 3 3 12 5 0.0 3 5 13 6 0.0 3 4 2 14 12 0.0 4 10 2 15 3 0.0 4 2 1 16 8 0.0 4 7 1 17 14 0.0 5 10 3 0 1 18 6 0.0 5 6 19 5 0.0 5 4 0 1 20 54 0.0 6 52 1 1 21 56 0.0 6 52 4 22 31 0.0 6 31 23 27 0.0 6 26 1 24 17 0.0 6 15 2 25 19 0.0 6 19 26 10 0.0 6 9 0 1 27 7 0.0 6 7 28 5 0.0 6 5 29 10 0.0 6 10 30 12 0.0 6 10 1 0 0 0 1 31 10 0.0 6 8 2 32 6 0.0 6 6 33 6 0.0 6 5 1 34 11 0.0 6 11 35 8 0.0 6 7 1 36 6 0.0 6 6 37 7 0.0 6 6 1 38 10 0.0 6 9 1 39 11 0.0 6 10 1 40 8 0.0 6 8 41 6 0.0 6 6 42 7 0.0 6 7 43 5 0.0 6 4 0 0 1 44 23 0.0 6 23 45 9 0.0 6 8 0 1 46 24 0.0 6 22 2 47 8 0.0 6 8 48 10 0.0 6 9 1 49 23 0.0 6 21 2 50 7 0.0 6 6 0 0 0 1 51 11 0.0 6 10 1 52 17 0.0 6 17 53 9 0.0 6 8 1 54 31 0.0 6 29 2 55 29 0.0 6 27 2 56 22 0.0 6 18 2 1 1 57 26 0.0 6 26 58 11 0.0 6 10 1 59 12 0.0 6 11 1 60 13 0.0 6 8 4 1 61 7 0.0 6 7 62 18 0.0 6 16 2 63 7 0.0 6 7 64 14 0.0 6 13 1 65 7 0.0 6 7 66 28 0.0 6 26 2 67 30 0.0 6 30 68 39 0.0 6 35 4 69 142 0.0 6 137 4 0 1 70 23 0.0 6 22 1 71 69 0.0 6 66 3 72 35 0.0 6 34 1 73 23 0.0 6 20 2 0 0 0 0 1 74 22 0.0 6 22 75 18 0.0 6 17 1 76 22 0.0 6 18 4 77 31 0.0 6 26 4 1 78 38 0.0 6 36 1 0 0 1 79 29 0.0 6 25 3 0 0 1 80 22 0.0 6 20 2 81 21 0.0 6 18 3 82 28 0.0 6 26 2 83 34 0.0 6 34 84 47 0.0 6 43 4 85 47 0.0 6 46 1 86 20 0.0 6 20 87 45 0.0 6 41 3 1 88 16 0.0 6 15 1 89 58 0.0 6 54 4 90 39 0.0 6 38 1 91 45 0.0 6 45 92 32 0.0 6 26 4 1 0 1 93 45 0.0 6 38 4 1 0 2 94 105 0.0 6 98 6 0 0 0 1 95 27 0.0 6 25 2 96 41 0.0 6 38 2 0 0 1 97 60 0.0 6 60 98 30 0.0 6 30 99 44 0.0 6 41 3 100 65 0.0 6 61 4 101 43 0.0 6 41 2 102 80 0.0 6 76 3 1 103 85 0.0 6 79 5 0 0 0 0 1 104 64 0.0 6 63 1 105 78 0.0 6 75 3 106 43 0.0 6 42 1 107 59 0.0 6 53 5 1 108 61 0.0 6 57 2 0 0 2 109 53 0.0 6 51 2 110 43 0.0 6 40 3 111 59 0.0 6 58 1 112 46 0.0 6 43 2 1 113 33 0.0 6 32 1 114 48 0.0 6 45 2 0 1 115 50 0.0 6 47 3 116 47 0.0 6 46 1 117 34 0.0 6 32 2 118 58 0.0 6 55 3 119 77 0.0 6 73 4 120 127 0.0 6 123 4 121 71 0.0 6 66 4 0 0 1 122 109 0.0 6 101 8 123 103 0.0 6 93 10 124 132 0.0 6 124 8 125 88 0.0 6 80 6 2 126 64 0.0 6 62 2 127 98 0.0 6 94 2 2 128 61 0.0 6 56 5 129 53 0.0 6 51 2 130 67 0.0 6 66 1 131 95 0.0 6 87 8 132 119 0.0 6 117 2 133 136 0.0 6 130 5 0 0 1 134 155 0.0 6 146 8 1 135 272 0.0 6 263 7 1 0 1 136 199 0.0 6 189 10 137 205 0.0 6 193 11 1 138 137 0.0 6 129 7 1 139 202 0.0 6 190 10 1 1 140 228 0.0 6 220 7 0 1 141 229 0.0 6 224 4 1 142 319 0.0 6 306 13 143 252 0.0 6 244 7 0 0 1 144 304 0.0 6 299 5 145 196 0.0 6 189 5 2 146 160 0.0 6 156 4 147 129 0.0 6 124 5 148 230 0.0 6 215 14 0 1 149 266 0.0 6 262 3 0 0 1 150 394 0.0 6 380 14 151 260 0.0 6 247 12 1 152 629 0.0 6 613 16 153 354 0.0 6 341 13 154 428 0.0 6 411 15 0 0 2 155 421 0.0 6 407 13 1 156 403 0.0 6 386 16 0 1 157 511 0.0 6 493 17 0 1 158 453 0.0 6 432 21 159 351 0.0 6 339 11 0 0 1 160 415 0.0 6 403 10 1 0 1 161 282 0.0 6 275 7 162 274 0.0 6 268 5 1 163 489 0.0 6 477 12 164 391 0.0 6 384 6 0 0 1 165 622 0.0 6 606 15 1 166 562 0.0 6 543 19 167 428 0.0 6 413 14 1 168 551 0.0 6 529 20 0 0 2 169 293 0.0 6 283 9 1 170 504 0.0 6 488 15 0 0 1 171 530 0.0 6 511 17 1 1 172 353 0.0 6 342 10 1 173 678 0.0 6 648 28 2 174 622 0.0 6 604 17 0 0 1 175 958 0.0 6 931 22 3 2 176 697 0.0 6 667 29 1 177 819 0.0 6 796 19 1 1 2 178 890 0.0 6 857 31 1 0 0 1 179 838 0.0 6 810 24 2 1 1 180 1182 0.0 6 1143 38 0 0 0 1 181 912 0.0 6 883 22 5 0 2 182 617 0.0 6 590 25 1 1 183 968 0.0 6 934 31 3 184 518 0.0 6 506 10 2 185 881 0.0 6 850 28 2 1 186 1611 0.0 6 1563 44 1 0 1 1 1 187 1446 0.0 6 1411 34 0 0 1 188 1503 0.0 6 1462 38 2 0 1 189 1197 0.0 6 1154 43 190 1649 0.0 6 1600 46 1 1 1 191 1521 0.0 6 1485 31 2 0 3 192 1034 0.0 6 1007 24 0 1 2 193 911 0.0 6 883 26 0 1 1 194 1234 0.0 6 1202 31 1 195 1261 0.0 6 1210 50 0 0 1 196 2175 0.0 6 2112 62 0 0 1 197 1106 0.0 6 1075 26 4 0 1 198 2209 0.0 6 2155 51 1 0 2 199 2207 0.0 6 2155 48 4 200 1375 0.0 6 1334 35 4 0 1 0 1 201 1681 0.0 6 1624 57 202 1108 0.0 6 1077 28 1 1 1 203 1066 0.0 6 1031 30 1 2 2 204 1261 0.0 6 1220 40 0 0 1 205 774 0.0 6 756 16 1 1 206 621 0.0 6 605 15 1 207 612 0.0 6 595 17 208 942 0.0 6 924 18 209 578 0.0 6 551 23 2 1 1 210 666 0.0 6 647 18 1 211 616 0.0 6 592 24 212 535 0.0 6 521 13 0 1 213 311 0.0 6 303 8 214 490 0.0 6 482 8 215 656 0.0 6 642 14 216 1073 0.0 6 1045 28 217 805 0.0 6 784 21 218 681 0.0 6 665 14 1 0 1 219 624 0.0 6 606 16 1 0 1 220 508 0.0 6 495 13 221 561 0.0 6 549 12 222 364 0.0 6 357 7 223 750 0.0 6 729 19 0 0 2 224 463 0.0 6 445 17 1 225 590 0.0 6 576 13 1 226 514 0.0 6 503 10 1 227 494 0.0 6 485 8 1 228 431 0.0 6 423 8 229 620 0.0 6 600 18 1 0 1 230 915 0.0 6 896 17 1 0 1 231 533 0.0 6 512 21 232 551 0.0 6 532 18 1 233 442 0.0 6 430 12 234 326 0.0 6 321 5 235 156 0.0 6 149 7 236 168 0.0 6 167 1 237 157 0.0 6 154 3 238 97 0.0 6 93 4 239 48 0.0 6 46 2 240 166 0.0 6 161 5 241 147 0.0 6 142 5 242 157 0.0 6 152 5 243 210 0.0 6 204 6 244 101 0.0 6 98 3 245 122 0.0 6 118 4 246 108 0.0 6 104 3 0 0 1 247 78 0.0 6 74 4 248 70 0.0 6 68 2 249 101 0.0 6 100 1 250 69 0.0 6 67 1 0 0 0 1 251 96 0.0 6 93 3 252 44 0.0 6 43 1 253 70 0.0 6 69 1 254 26 0.0 6 24 2 255 25 0.0 6 25 256 7 0.0 6 6 1 257 4 0.0 6 4 258 1 0.0 6 1 260 1 0.0 6 1 265 1 0.0 6 1 266 1 0.0 6 1 279 7 0.0 6 7 280 72 0.0 6 68 4 281 378 0.0 6 365 11 2 282 3 0.0 6 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 11 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 77 2 0.0 6 2 84 1 0.0 6 1 95 1 0.0 6 1 108 1 0.0 6 1 111 1 0.0 6 1 119 2 0.0 6 2 136 1 0.0 6 1 178 1 0.0 6 1 184 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_06_41_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_06_268_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_06_41_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_06_268_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.68 s (26 µs/read; 2.34 M reads/minute). === Summary === Total read pairs processed: 65,426 Read 1 with adapter: 65,424 (100.0%) Read 2 with adapter: 65,400 (100.0%) == Read fate breakdown == Pairs that were too short: 34 (0.1%) Pairs discarded as untrimmed: 28 (0.0%) Pairs written (passing filters): 65,364 (99.9%) Total basepairs processed: 28,357,382 bp Read 1: 15,993,766 bp Read 2: 12,363,616 bp Total written (filtered): 3,946,426 bp (13.9%) Read 1: 2,019,156 bp Read 2: 1,927,270 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 64125 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 3 16.0 1 1 0 2 7 2 4.0 2 0 0 2 8 2 1.0 2 2 9 2 0.2 2 1 1 10 3 0.1 3 3 12 1 0.0 3 1 15 2 0.0 4 2 17 1 0.0 5 0 1 18 1 0.0 5 1 19 3 0.0 5 1 2 20 107 0.0 6 102 5 21 52 0.0 6 50 2 22 48 0.0 6 47 0 1 23 30 0.0 6 29 1 24 20 0.0 6 20 25 19 0.0 6 19 26 10 0.0 6 7 2 1 27 8 0.0 6 8 28 15 0.0 6 15 29 10 0.0 6 9 1 30 8 0.0 6 8 31 4 0.0 6 4 32 5 0.0 6 5 33 3 0.0 6 3 34 6 0.0 6 6 36 3 0.0 6 3 37 7 0.0 6 7 38 5 0.0 6 5 39 4 0.0 6 4 40 6 0.0 6 6 41 4 0.0 6 4 42 6 0.0 6 6 43 5 0.0 6 5 44 9 0.0 6 9 45 1 0.0 6 1 46 6 0.0 6 6 47 9 0.0 6 9 48 6 0.0 6 6 49 6 0.0 6 6 50 3 0.0 6 3 51 12 0.0 6 11 1 52 6 0.0 6 6 53 9 0.0 6 9 54 5 0.0 6 5 55 5 0.0 6 5 56 8 0.0 6 8 57 5 0.0 6 5 58 3 0.0 6 3 59 5 0.0 6 5 60 3 0.0 6 3 61 7 0.0 6 5 2 62 2 0.0 6 2 63 9 0.0 6 8 1 64 4 0.0 6 4 65 4 0.0 6 4 66 7 0.0 6 7 67 8 0.0 6 7 1 68 4 0.0 6 4 69 4 0.0 6 4 70 5 0.0 6 5 71 8 0.0 6 8 72 10 0.0 6 9 1 73 7 0.0 6 7 74 8 0.0 6 8 75 8 0.0 6 8 76 8 0.0 6 8 77 6 0.0 6 6 78 7 0.0 6 5 2 79 7 0.0 6 6 1 80 7 0.0 6 7 81 3 0.0 6 3 82 5 0.0 6 5 83 10 0.0 6 10 84 10 0.0 6 10 85 13 0.0 6 12 1 86 15 0.0 6 14 1 87 7 0.0 6 7 88 8 0.0 6 8 89 9 0.0 6 9 90 7 0.0 6 7 91 11 0.0 6 11 92 8 0.0 6 7 1 93 5 0.0 6 4 1 94 6 0.0 6 6 95 5 0.0 6 5 96 5 0.0 6 4 1 97 15 0.0 6 14 1 98 8 0.0 6 8 99 13 0.0 6 13 100 17 0.0 6 16 1 101 9 0.0 6 9 102 18 0.0 6 18 103 13 0.0 6 11 1 1 104 14 0.0 6 13 1 105 24 0.0 6 23 1 106 27 0.0 6 26 1 107 17 0.0 6 17 108 15 0.0 6 14 1 109 19 0.0 6 17 2 110 12 0.0 6 11 1 111 10 0.0 6 10 112 11 0.0 6 11 113 20 0.0 6 20 114 22 0.0 6 22 115 8 0.0 6 7 1 116 21 0.0 6 20 1 117 21 0.0 6 21 118 25 0.0 6 25 119 20 0.0 6 20 120 16 0.0 6 16 121 30 0.0 6 27 2 0 0 0 1 122 32 0.0 6 31 1 123 17 0.0 6 16 1 124 20 0.0 6 20 125 14 0.0 6 14 126 18 0.0 6 18 127 32 0.0 6 31 1 128 23 0.0 6 23 129 37 0.0 6 35 2 130 38 0.0 6 38 131 32 0.0 6 31 0 1 132 34 0.0 6 33 1 133 40 0.0 6 40 134 23 0.0 6 23 135 23 0.0 6 22 0 0 0 0 0 1 136 32 0.0 6 32 137 33 0.0 6 33 138 32 0.0 6 31 0 0 0 0 1 139 49 0.0 6 48 0 1 140 151 0.0 6 151 141 281 0.0 6 280 1 142 418 0.0 6 418 143 290 0.0 6 290 144 316 0.0 6 315 1 145 428 0.0 6 425 2 1 146 339 0.0 6 338 0 1 147 314 0.0 6 313 1 148 344 0.0 6 341 3 149 345 0.0 6 342 2 0 0 1 150 369 0.0 6 369 151 314 0.0 6 314 152 261 0.0 6 259 1 1 153 240 0.0 6 239 1 154 271 0.0 6 269 2 155 268 0.0 6 267 1 156 215 0.0 6 212 2 0 0 0 0 1 157 259 0.0 6 259 158 251 0.0 6 250 1 159 261 0.0 6 260 1 160 296 0.0 6 295 1 161 237 0.0 6 237 162 248 0.0 6 245 3 163 261 0.0 6 261 164 219 0.0 6 219 165 203 0.0 6 203 166 285 0.0 6 285 167 229 0.0 6 228 1 168 222 0.0 6 222 169 245 0.0 6 245 170 222 0.0 6 220 2 171 272 0.0 6 271 1 172 261 0.0 6 260 1 173 287 0.0 6 287 174 231 0.0 6 226 5 175 315 0.0 6 312 2 1 176 274 0.0 6 272 1 0 1 177 278 0.0 6 277 1 178 671 0.0 6 666 5 179 273 0.0 6 272 1 180 253 0.0 6 250 2 1 181 260 0.0 6 259 1 182 261 0.0 6 258 3 183 265 0.0 6 262 3 184 276 0.0 6 275 1 185 272 0.0 6 271 1 186 266 0.0 6 265 1 187 319 0.0 6 316 3 188 305 0.0 6 305 189 304 0.0 6 302 2 190 323 0.0 6 320 3 191 336 0.0 6 333 3 192 329 0.0 6 324 5 193 389 0.0 6 388 1 194 392 0.0 6 391 1 195 398 0.0 6 392 5 1 196 456 0.0 6 450 5 0 0 0 1 197 492 0.0 6 482 10 198 612 0.0 6 606 6 199 677 0.0 6 672 3 0 0 0 0 2 200 687 0.0 6 685 1 1 201 578 0.0 6 574 4 202 561 0.0 6 558 3 203 537 0.0 6 535 1 1 204 476 0.0 6 472 4 205 566 0.0 6 562 4 206 556 0.0 6 546 10 207 678 0.0 6 674 3 1 208 773 0.0 6 769 2 0 2 209 612 0.0 6 608 4 210 624 0.0 6 615 7 0 0 2 211 514 0.0 6 509 5 212 566 0.0 6 562 4 213 664 0.0 6 658 6 214 735 0.0 6 730 4 0 0 0 1 215 731 0.0 6 723 6 0 1 1 216 770 0.0 6 765 4 1 217 998 0.0 6 988 8 1 0 0 0 1 218 773 0.0 6 767 4 0 1 0 1 219 746 0.0 6 735 9 1 0 0 1 220 823 0.0 6 821 1 1 221 706 0.0 6 703 3 222 605 0.0 6 597 6 1 0 0 1 223 606 0.0 6 602 2 1 0 0 0 1 224 623 0.0 6 619 4 225 706 0.0 6 701 5 226 654 0.0 6 652 2 227 768 0.0 6 764 3 1 228 779 0.0 6 774 5 229 575 0.0 6 572 3 230 621 0.0 6 618 3 231 528 0.0 6 526 2 232 655 0.0 6 651 4 233 593 0.0 6 589 4 234 575 0.0 6 572 3 235 601 0.0 6 599 2 236 532 0.0 6 528 3 1 237 485 0.0 6 483 2 238 552 0.0 6 549 2 0 0 0 1 239 516 0.0 6 510 5 0 0 0 0 1 240 520 0.0 6 518 2 241 509 0.0 6 507 2 242 481 0.0 6 479 1 0 0 0 1 243 495 0.0 6 491 4 244 494 0.0 6 493 1 245 451 0.0 6 445 5 0 0 0 0 1 246 381 0.0 6 378 3 247 370 0.0 6 368 2 248 302 0.0 6 298 3 0 1 249 338 0.0 6 334 4 250 378 0.0 6 377 1 251 348 0.0 6 345 3 252 366 0.0 6 362 4 253 358 0.0 6 357 1 254 306 0.0 6 305 1 255 363 0.0 6 358 5 256 286 0.0 6 286 257 268 0.0 6 267 0 0 0 0 0 1 258 242 0.0 6 239 2 0 1 259 324 0.0 6 324 260 304 0.0 6 301 3 261 311 0.0 6 304 7 262 271 0.0 6 269 2 263 218 0.0 6 216 2 264 213 0.0 6 213 265 232 0.0 6 228 4 266 202 0.0 6 202 267 207 0.0 6 206 1 268 223 0.0 6 218 4 0 0 0 1 269 199 0.0 6 197 1 0 1 270 201 0.0 6 200 1 271 256 0.0 6 255 1 272 221 0.0 6 220 1 273 164 0.0 6 161 3 274 176 0.0 6 175 1 275 179 0.0 6 174 3 2 276 219 0.0 6 216 3 277 330 0.0 6 325 5 278 344 0.0 6 340 4 279 411 0.0 6 405 6 280 1290 0.0 6 1268 19 2 1 281 4338 0.0 6 4288 49 0 0 0 1 282 17 0.0 6 17 283 1 0.0 6 1 284 3 0.0 6 2 0 0 1 286 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1299 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 53 1 0.0 6 1 60 4 0.0 6 3 1 61 53 0.0 6 50 3 63 1 0.0 6 1 67 2 0.0 6 1 0 0 0 0 0 1 68 1 0.0 6 1 69 1 0.0 6 1 70 3 0.0 6 3 71 11 0.0 6 11 72 7 0.0 6 7 73 7 0.0 6 7 74 7 0.0 6 7 75 12 0.0 6 12 76 7 0.0 6 7 77 9 0.0 6 9 78 10 0.0 6 10 79 8 0.0 6 8 80 6 0.0 6 6 81 6 0.0 6 6 82 5 0.0 6 5 83 7 0.0 6 7 84 6 0.0 6 6 85 2 0.0 6 2 86 4 0.0 6 4 87 3 0.0 6 3 88 9 0.0 6 9 89 5 0.0 6 5 90 7 0.0 6 7 91 9 0.0 6 9 92 7 0.0 6 7 93 9 0.0 6 9 94 6 0.0 6 6 95 5 0.0 6 5 96 5 0.0 6 5 97 3 0.0 6 3 98 7 0.0 6 7 99 3 0.0 6 3 100 5 0.0 6 5 101 4 0.0 6 4 102 7 0.0 6 7 103 8 0.0 6 8 104 5 0.0 6 5 105 5 0.0 6 5 106 5 0.0 6 5 107 7 0.0 6 7 108 16 0.0 6 16 109 10 0.0 6 10 110 8 0.0 6 8 111 3 0.0 6 3 112 4 0.0 6 4 113 4 0.0 6 4 114 5 0.0 6 5 115 7 0.0 6 7 116 2 0.0 6 2 117 4 0.0 6 4 118 5 0.0 6 5 119 4 0.0 6 4 120 4 0.0 6 4 121 4 0.0 6 4 122 8 0.0 6 8 123 8 0.0 6 8 124 2 0.0 6 2 125 5 0.0 6 5 126 6 0.0 6 6 127 3 0.0 6 3 128 5 0.0 6 5 129 3 0.0 6 3 130 4 0.0 6 4 131 6 0.0 6 6 132 9 0.0 6 9 133 6 0.0 6 6 134 6 0.0 6 6 135 9 0.0 6 9 136 9 0.0 6 9 137 4 0.0 6 4 138 9 0.0 6 9 139 6 0.0 6 6 140 2 0.0 6 2 141 6 0.0 6 6 142 5 0.0 6 5 143 1 0.0 6 1 144 7 0.0 6 7 145 6 0.0 6 6 146 7 0.0 6 7 147 5 0.0 6 5 148 8 0.0 6 8 149 4 0.0 6 4 150 4 0.0 6 4 151 4 0.0 6 4 152 8 0.0 6 8 153 5 0.0 6 5 154 4 0.0 6 4 155 9 0.0 6 9 156 9 0.0 6 9 157 10 0.0 6 10 158 2 0.0 6 2 159 9 0.0 6 9 160 7 0.0 6 7 161 13 0.0 6 13 162 12 0.0 6 12 163 2 0.0 6 2 164 6 0.0 6 6 165 8 0.0 6 8 166 4 0.0 6 4 167 7 0.0 6 7 168 7 0.0 6 7 169 4 0.0 6 4 170 7 0.0 6 7 171 10 0.0 6 10 172 5 0.0 6 5 173 5 0.0 6 5 174 8 0.0 6 8 175 5 0.0 6 5 176 6 0.0 6 6 177 7 0.0 6 7 178 2 0.0 6 2 179 5 0.0 6 5 180 5 0.0 6 5 181 4 0.0 6 4 182 10 0.0 6 10 183 9 0.0 6 9 184 5 0.0 6 5 185 10 0.0 6 10 186 14 0.0 6 14 187 13 0.0 6 13 188 5 0.0 6 5 189 5 0.0 6 5 190 14 0.0 6 14 191 10 0.0 6 10 192 6 0.0 6 6 193 9 0.0 6 9 194 6 0.0 6 6 195 10 0.0 6 10 196 10 0.0 6 10 197 7 0.0 6 7 198 11 0.0 6 11 199 7 0.0 6 7 200 7 0.0 6 7 201 7 0.0 6 7 202 5 0.0 6 5 203 9 0.0 6 9 204 14 0.0 6 14 205 8 0.0 6 8 206 10 0.0 6 10 207 4 0.0 6 4 208 7 0.0 6 7 209 4 0.0 6 4 210 6 0.0 6 6 211 8 0.0 6 8 212 4 0.0 6 4 213 10 0.0 6 10 214 9 0.0 6 9 215 4 0.0 6 4 216 7 0.0 6 7 217 6 0.0 6 6 218 6 0.0 6 6 219 6 0.0 6 6 220 3 0.0 6 3 221 7 0.0 6 7 222 3 0.0 6 3 223 4 0.0 6 4 224 7 0.0 6 7 225 4 0.0 6 4 226 10 0.0 6 10 227 9 0.0 6 9 228 7 0.0 6 7 229 8 0.0 6 8 230 10 0.0 6 10 231 6 0.0 6 6 232 4 0.0 6 4 233 5 0.0 6 5 234 4 0.0 6 4 235 6 0.0 6 6 236 5 0.0 6 5 237 10 0.0 6 10 238 7 0.0 6 7 239 4 0.0 6 4 240 4 0.0 6 4 241 5 0.0 6 5 242 5 0.0 6 5 243 2 0.0 6 2 244 6 0.0 6 6 245 1 0.0 6 1 246 5 0.0 6 5 247 6 0.0 6 6 248 7 0.0 6 7 249 2 0.0 6 2 250 2 0.0 6 2 251 3 0.0 6 3 252 3 0.0 6 3 253 1 0.0 6 1 254 3 0.0 6 3 255 3 0.0 6 2 1 256 6 0.0 6 6 257 1 0.0 6 1 258 3 0.0 6 3 259 6 0.0 6 6 260 5 0.0 6 5 261 1 0.0 6 1 263 3 0.0 6 3 264 1 0.0 6 1 265 2 0.0 6 2 266 4 0.0 6 4 267 1 0.0 6 1 268 1 0.0 6 1 269 2 0.0 6 2 270 1 0.0 6 1 271 2 0.0 6 2 272 2 0.0 6 2 273 6 0.0 6 6 274 3 0.0 6 3 275 1 0.0 6 1 276 1 0.0 6 1 278 2 0.0 6 2 279 1 0.0 6 1 280 1 0.0 6 1 281 1 0.0 6 1 282 2 0.0 6 2 283 1 0.0 6 1 284 1 0.0 6 1 285 1 0.0 6 1 286 1 0.0 6 1 287 2 0.0 6 2 294 1 0.0 6 1 295 1 0.0 6 1 297 1 0.0 6 1 304 1 0.0 6 1 307 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 64443 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 16.0 1 1 7 1 4.0 2 1 10 1 0.1 3 1 11 3 0.0 3 3 12 5 0.0 3 5 13 2 0.0 3 1 1 14 10 0.0 4 10 15 3 0.0 4 3 16 4 0.0 4 4 17 22 0.0 5 20 2 18 5 0.0 5 4 1 19 50 0.0 5 48 2 20 81 0.0 6 77 4 21 60 0.0 6 57 3 22 32 0.0 6 30 2 23 28 0.0 6 28 24 25 0.0 6 24 1 25 20 0.0 6 19 1 26 15 0.0 6 14 1 27 15 0.0 6 14 1 28 15 0.0 6 13 2 29 21 0.0 6 18 3 30 15 0.0 6 11 3 0 0 1 31 9 0.0 6 6 3 32 12 0.0 6 11 1 33 16 0.0 6 15 0 1 34 16 0.0 6 13 3 35 17 0.0 6 14 2 0 1 36 15 0.0 6 13 2 37 10 0.0 6 10 38 11 0.0 6 11 39 13 0.0 6 12 1 40 9 0.0 6 9 41 8 0.0 6 8 42 12 0.0 6 12 43 17 0.0 6 16 1 44 15 0.0 6 14 1 45 18 0.0 6 16 2 46 11 0.0 6 10 0 0 1 47 18 0.0 6 17 0 0 0 0 0 1 48 15 0.0 6 14 1 49 18 0.0 6 15 1 0 0 1 0 1 50 19 0.0 6 16 1 1 0 0 1 51 12 0.0 6 12 52 17 0.0 6 16 1 53 18 0.0 6 18 54 20 0.0 6 18 2 55 24 0.0 6 24 56 21 0.0 6 15 4 0 1 0 1 57 27 0.0 6 23 3 0 1 58 18 0.0 6 17 0 1 59 20 0.0 6 18 2 60 28 0.0 6 25 3 61 28 0.0 6 26 2 62 29 0.0 6 27 1 0 0 1 63 31 0.0 6 27 3 1 64 16 0.0 6 14 2 65 25 0.0 6 23 2 66 24 0.0 6 24 67 20 0.0 6 17 2 1 68 30 0.0 6 30 69 43 0.0 6 40 1 1 0 0 1 70 131 0.0 6 122 6 2 0 0 1 71 47 0.0 6 44 3 72 64 0.0 6 62 2 73 35 0.0 6 34 1 74 51 0.0 6 49 1 0 0 0 1 75 37 0.0 6 36 0 0 0 0 1 76 28 0.0 6 27 0 0 0 0 1 77 40 0.0 6 40 78 36 0.0 6 35 1 79 41 0.0 6 41 80 47 0.0 6 45 2 81 47 0.0 6 44 3 82 53 0.0 6 48 4 0 1 83 54 0.0 6 50 3 1 84 50 0.0 6 47 3 85 59 0.0 6 56 3 86 75 0.0 6 71 4 87 51 0.0 6 48 1 0 1 0 1 88 46 0.0 6 41 5 89 33 0.0 6 32 1 90 51 0.0 6 46 2 0 1 1 0 1 91 36 0.0 6 35 1 92 51 0.0 6 48 3 93 60 0.0 6 57 1 1 0 1 94 82 0.0 6 80 1 0 0 1 95 64 0.0 6 61 2 0 1 96 81 0.0 6 79 2 97 71 0.0 6 66 3 1 0 0 0 1 98 74 0.0 6 71 2 0 0 1 99 93 0.0 6 92 1 100 96 0.0 6 92 3 1 101 67 0.0 6 62 3 0 0 2 102 82 0.0 6 79 2 1 103 73 0.0 6 68 3 0 0 0 2 104 80 0.0 6 77 3 105 99 0.0 6 98 1 106 110 0.0 6 108 0 1 1 107 183 0.0 6 177 6 108 99 0.0 6 92 4 1 0 0 2 109 97 0.0 6 94 2 0 0 1 110 109 0.0 6 105 1 0 1 1 0 1 111 114 0.0 6 107 6 1 112 95 0.0 6 93 1 1 113 138 0.0 6 135 1 2 114 116 0.0 6 113 3 115 167 0.0 6 161 5 0 0 0 0 1 116 133 0.0 6 131 2 117 152 0.0 6 148 3 0 1 118 197 0.0 6 194 2 1 119 135 0.0 6 133 2 120 178 0.0 6 173 5 121 175 0.0 6 163 8 1 0 2 0 1 122 166 0.0 6 161 5 123 194 0.0 6 189 5 124 152 0.0 6 150 2 125 208 0.0 6 206 0 0 0 0 1 1 126 206 0.0 6 203 2 0 0 0 0 1 127 240 0.0 6 234 6 128 274 0.0 6 267 7 129 300 0.0 6 285 14 1 130 245 0.0 6 239 5 0 0 1 131 326 0.0 6 319 6 1 132 310 0.0 6 303 7 133 327 0.0 6 319 4 1 0 1 1 1 134 374 0.0 6 372 2 135 419 0.0 6 407 9 2 0 0 0 1 136 448 0.0 6 439 7 0 1 0 0 1 137 533 0.0 6 519 13 0 0 0 1 138 624 0.0 6 614 8 0 2 139 621 0.0 6 614 5 1 0 0 1 140 683 0.0 6 672 7 1 1 0 1 1 141 603 0.0 6 597 4 0 0 0 1 1 142 718 0.0 6 705 13 143 774 0.0 6 762 8 1 0 2 1 144 788 0.0 6 782 6 145 836 0.0 6 824 11 0 0 0 1 146 797 0.0 6 783 10 1 0 2 1 147 840 0.0 6 831 8 0 0 0 1 148 895 0.0 6 888 5 0 1 1 149 937 0.0 6 919 17 1 150 923 0.0 6 904 16 0 0 3 151 985 0.0 6 969 15 0 0 0 0 1 152 1201 0.0 6 1177 20 0 0 2 0 2 153 1513 0.0 6 1481 26 1 0 2 2 1 154 1088 0.0 6 1065 23 155 1388 0.0 6 1356 24 3 2 1 0 2 156 1320 0.0 6 1304 12 1 1 0 0 2 157 1087 0.0 6 1065 18 0 1 0 2 1 158 1109 0.0 6 1091 17 1 159 931 0.0 6 918 10 0 0 0 3 160 1118 0.0 6 1103 15 161 1051 0.0 6 1032 18 0 1 162 919 0.0 6 910 8 1 163 955 0.0 6 939 14 0 1 0 0 1 164 892 0.0 6 873 13 0 2 3 0 1 165 774 0.0 6 759 15 166 839 0.0 6 832 7 167 806 0.0 6 795 10 0 0 0 0 1 168 775 0.0 6 759 12 0 0 3 0 1 169 973 0.0 6 961 9 0 1 2 170 768 0.0 6 761 6 0 0 0 1 171 945 0.0 6 930 12 2 0 0 1 172 796 0.0 6 789 5 0 0 1 1 173 842 0.0 6 835 6 1 174 930 0.0 6 917 12 0 0 1 175 986 0.0 6 974 11 1 176 930 0.0 6 920 8 1 1 177 734 0.0 6 722 12 178 758 0.0 6 751 6 0 1 179 886 0.0 6 881 5 180 647 0.0 6 642 4 0 0 0 1 181 673 0.0 6 662 10 0 0 0 1 182 520 0.0 6 515 3 1 0 0 1 183 531 0.0 6 524 6 0 0 1 184 475 0.0 6 467 8 185 455 0.0 6 448 7 186 705 0.0 6 694 9 0 0 1 1 187 909 0.0 6 902 5 0 2 188 865 0.0 6 855 9 0 0 0 1 189 514 0.0 6 506 8 190 626 0.0 6 615 10 0 0 0 0 1 191 552 0.0 6 544 6 0 0 0 1 1 192 503 0.0 6 493 8 0 1 1 193 425 0.0 6 420 5 194 502 0.0 6 489 11 0 0 1 0 1 195 427 0.0 6 423 3 1 196 475 0.0 6 470 5 197 337 0.0 6 331 4 0 0 1 0 1 198 337 0.0 6 329 7 0 0 0 0 1 199 552 0.0 6 542 9 0 0 1 200 330 0.0 6 327 3 201 347 0.0 6 344 3 202 272 0.0 6 266 4 1 0 0 0 1 203 268 0.0 6 265 3 204 222 0.0 6 219 2 0 0 0 1 205 196 0.0 6 194 2 206 186 0.0 6 185 1 207 182 0.0 6 178 4 208 152 0.0 6 146 5 1 209 177 0.0 6 175 1 0 0 1 210 159 0.0 6 157 2 211 153 0.0 6 150 3 212 182 0.0 6 177 5 213 97 0.0 6 97 214 151 0.0 6 148 3 215 128 0.0 6 127 1 216 124 0.0 6 122 1 0 0 0 0 1 217 162 0.0 6 161 0 0 1 218 113 0.0 6 111 2 219 106 0.0 6 106 220 66 0.0 6 64 2 221 108 0.0 6 106 2 222 53 0.0 6 53 223 48 0.0 6 47 1 224 84 0.0 6 82 2 225 81 0.0 6 78 3 226 62 0.0 6 59 3 227 50 0.0 6 50 228 41 0.0 6 40 0 1 229 59 0.0 6 59 230 26 0.0 6 25 1 231 34 0.0 6 34 232 49 0.0 6 47 2 233 31 0.0 6 30 1 234 21 0.0 6 21 235 25 0.0 6 25 236 8 0.0 6 8 237 16 0.0 6 16 238 5 0.0 6 5 239 6 0.0 6 6 240 7 0.0 6 7 241 8 0.0 6 8 242 21 0.0 6 21 243 21 0.0 6 21 244 22 0.0 6 22 245 6 0.0 6 5 1 246 5 0.0 6 5 247 3 0.0 6 3 248 1 0.0 6 1 249 5 0.0 6 5 250 3 0.0 6 3 251 6 0.0 6 6 253 2 0.0 6 2 254 4 0.0 6 4 256 1 0.0 6 1 257 1 0.0 6 1 261 1 0.0 6 1 280 4 0.0 6 4 281 17 0.0 6 17 282 87 0.0 6 86 0 0 0 0 1 287 1 0.0 6 0 0 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 957 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 58 13 0.0 6 13 63 3 0.0 6 3 65 1 0.0 6 1 66 1 0.0 6 1 68 11 0.0 6 11 69 1 0.0 6 1 70 7 0.0 6 7 71 16 0.0 6 16 72 5 0.0 6 5 73 11 0.0 6 11 74 14 0.0 6 14 75 2 0.0 6 2 76 18 0.0 6 18 77 7 0.0 6 7 78 17 0.0 6 16 0 0 0 1 79 13 0.0 6 13 80 10 0.0 6 10 81 13 0.0 6 13 82 10 0.0 6 10 83 8 0.0 6 8 84 9 0.0 6 9 85 8 0.0 6 8 86 8 0.0 6 8 87 8 0.0 6 8 88 12 0.0 6 12 89 13 0.0 6 13 90 4 0.0 6 4 91 2 0.0 6 2 92 6 0.0 6 6 93 2 0.0 6 2 94 7 0.0 6 7 95 6 0.0 6 6 96 13 0.0 6 12 1 97 5 0.0 6 5 98 6 0.0 6 6 99 8 0.0 6 8 100 14 0.0 6 14 101 9 0.0 6 9 102 9 0.0 6 9 103 3 0.0 6 3 104 7 0.0 6 7 105 9 0.0 6 9 106 5 0.0 6 5 107 12 0.0 6 12 108 8 0.0 6 8 109 9 0.0 6 9 110 5 0.0 6 5 111 5 0.0 6 5 112 7 0.0 6 7 113 4 0.0 6 4 114 6 0.0 6 6 115 6 0.0 6 6 116 8 0.0 6 8 117 8 0.0 6 8 118 2 0.0 6 2 119 3 0.0 6 3 120 9 0.0 6 9 121 3 0.0 6 3 122 5 0.0 6 5 123 5 0.0 6 5 124 5 0.0 6 5 125 2 0.0 6 2 126 3 0.0 6 3 127 5 0.0 6 5 128 5 0.0 6 5 129 3 0.0 6 3 130 8 0.0 6 8 131 10 0.0 6 10 132 5 0.0 6 5 133 3 0.0 6 3 134 9 0.0 6 9 135 7 0.0 6 7 136 5 0.0 6 5 137 8 0.0 6 8 138 3 0.0 6 3 139 7 0.0 6 7 140 6 0.0 6 6 141 5 0.0 6 5 142 7 0.0 6 7 143 4 0.0 6 4 144 5 0.0 6 5 145 1 0.0 6 1 146 6 0.0 6 6 147 3 0.0 6 3 148 6 0.0 6 6 149 4 0.0 6 4 150 6 0.0 6 6 151 1 0.0 6 1 152 5 0.0 6 5 153 6 0.0 6 6 154 10 0.0 6 10 155 7 0.0 6 7 156 7 0.0 6 7 157 5 0.0 6 5 158 6 0.0 6 6 159 8 0.0 6 8 160 10 0.0 6 10 161 7 0.0 6 7 162 8 0.0 6 8 163 11 0.0 6 11 164 13 0.0 6 13 165 5 0.0 6 5 166 7 0.0 6 7 167 3 0.0 6 3 168 7 0.0 6 7 169 7 0.0 6 7 170 4 0.0 6 4 171 10 0.0 6 10 172 5 0.0 6 5 173 4 0.0 6 4 174 5 0.0 6 5 175 8 0.0 6 8 176 2 0.0 6 2 177 6 0.0 6 6 178 8 0.0 6 8 179 5 0.0 6 5 180 10 0.0 6 10 181 8 0.0 6 8 182 7 0.0 6 7 183 10 0.0 6 10 184 8 0.0 6 8 185 6 0.0 6 6 186 2 0.0 6 2 188 6 0.0 6 6 189 8 0.0 6 8 190 9 0.0 6 9 191 4 0.0 6 4 192 3 0.0 6 3 193 5 0.0 6 5 194 5 0.0 6 5 195 1 0.0 6 1 196 1 0.0 6 1 197 5 0.0 6 5 198 5 0.0 6 5 199 5 0.0 6 5 200 2 0.0 6 1 1 201 1 0.0 6 1 202 2 0.0 6 2 203 4 0.0 6 4 204 3 0.0 6 3 205 3 0.0 6 3 206 4 0.0 6 4 207 3 0.0 6 3 208 4 0.0 6 3 0 0 0 0 0 1 209 1 0.0 6 1 210 3 0.0 6 3 211 1 0.0 6 1 212 1 0.0 6 1 214 3 0.0 6 3 215 4 0.0 6 4 216 3 0.0 6 3 217 1 0.0 6 1 218 3 0.0 6 3 220 2 0.0 6 2 221 1 0.0 6 1 222 1 0.0 6 1 223 2 0.0 6 2 224 1 0.0 6 1 225 1 0.0 6 1 230 1 0.0 6 1 231 1 0.0 6 1 233 1 0.0 6 1 234 1 0.0 6 1 265 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_15_42_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_15_269_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_15_42_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_15_269_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.11 s (33 µs/read; 1.79 M reads/minute). === Summary === Total read pairs processed: 62,999 Read 1 with adapter: 62,996 (100.0%) Read 2 with adapter: 62,959 (99.9%) == Read fate breakdown == Pairs that were too short: 1 (0.0%) Pairs discarded as untrimmed: 43 (0.1%) Pairs written (passing filters): 62,955 (99.9%) Total basepairs processed: 31,700,929 bp Read 1: 18,235,423 bp Read 2: 13,465,506 bp Total written (filtered): 3,904,073 bp (12.3%) Read 1: 1,889,328 bp Read 2: 2,014,745 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 62977 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 2 15.4 1 0 0 2 7 1 3.8 2 1 9 2 0.2 2 2 12 1 0.0 3 1 20 10 0.0 6 10 21 10 0.0 6 9 1 22 3 0.0 6 3 23 2 0.0 6 2 24 3 0.0 6 3 25 7 0.0 6 7 26 8 0.0 6 7 1 27 3 0.0 6 3 28 6 0.0 6 6 29 1 0.0 6 1 30 2 0.0 6 2 31 1 0.0 6 1 32 2 0.0 6 0 1 1 33 4 0.0 6 3 1 34 3 0.0 6 3 35 5 0.0 6 3 2 36 1 0.0 6 1 38 1 0.0 6 1 39 5 0.0 6 3 2 40 2 0.0 6 1 1 41 3 0.0 6 3 42 1 0.0 6 1 43 1 0.0 6 1 44 5 0.0 6 5 45 8 0.0 6 7 1 46 3 0.0 6 3 47 1 0.0 6 1 48 1 0.0 6 0 1 49 4 0.0 6 4 50 2 0.0 6 2 53 3 0.0 6 3 54 3 0.0 6 2 1 55 1 0.0 6 1 56 6 0.0 6 6 57 4 0.0 6 4 58 3 0.0 6 2 1 59 2 0.0 6 1 1 60 1 0.0 6 1 62 5 0.0 6 5 63 1 0.0 6 1 64 1 0.0 6 1 66 1 0.0 6 1 67 2 0.0 6 2 68 3 0.0 6 3 69 3 0.0 6 3 71 2 0.0 6 2 72 1 0.0 6 1 73 6 0.0 6 6 75 1 0.0 6 1 77 7 0.0 6 7 78 4 0.0 6 4 79 4 0.0 6 4 80 2 0.0 6 2 81 2 0.0 6 2 83 1 0.0 6 1 84 1 0.0 6 1 85 2 0.0 6 2 86 9 0.0 6 9 87 3 0.0 6 3 89 1 0.0 6 1 90 2 0.0 6 2 91 7 0.0 6 7 92 4 0.0 6 4 93 5 0.0 6 5 96 1 0.0 6 1 97 4 0.0 6 4 98 3 0.0 6 3 99 2 0.0 6 2 101 4 0.0 6 4 102 24 0.0 6 24 103 11 0.0 6 11 104 5 0.0 6 5 105 6 0.0 6 5 1 106 5 0.0 6 5 107 16 0.0 6 16 108 11 0.0 6 10 1 109 8 0.0 6 8 110 2 0.0 6 2 111 14 0.0 6 14 112 7 0.0 6 7 113 14 0.0 6 12 2 114 8 0.0 6 8 115 6 0.0 6 6 116 13 0.0 6 12 1 117 7 0.0 6 7 118 10 0.0 6 8 2 119 6 0.0 6 6 120 3 0.0 6 3 121 7 0.0 6 7 122 14 0.0 6 14 123 17 0.0 6 15 2 124 11 0.0 6 10 1 125 6 0.0 6 5 1 126 9 0.0 6 9 127 4 0.0 6 3 1 128 7 0.0 6 7 129 6 0.0 6 6 130 3 0.0 6 3 131 8 0.0 6 8 132 3 0.0 6 3 133 9 0.0 6 6 3 134 14 0.0 6 13 1 135 9 0.0 6 9 136 6 0.0 6 4 2 137 14 0.0 6 12 2 138 9 0.0 6 8 1 139 9 0.0 6 9 140 12 0.0 6 12 141 8 0.0 6 8 142 16 0.0 6 16 143 20 0.0 6 18 2 144 47 0.0 6 47 145 23 0.0 6 21 1 1 146 16 0.0 6 16 147 23 0.0 6 23 148 59 0.0 6 57 2 149 20 0.0 6 20 150 22 0.0 6 21 1 151 23 0.0 6 22 1 152 28 0.0 6 25 3 153 16 0.0 6 16 154 22 0.0 6 21 1 155 31 0.0 6 30 1 156 17 0.0 6 16 1 157 56 0.0 6 56 158 44 0.0 6 43 1 159 44 0.0 6 43 1 160 47 0.0 6 45 2 161 38 0.0 6 35 3 162 18 0.0 6 17 1 163 28 0.0 6 27 1 164 60 0.0 6 60 165 46 0.0 6 42 4 166 23 0.0 6 23 167 16 0.0 6 16 168 29 0.0 6 29 169 19 0.0 6 19 170 26 0.0 6 26 171 26 0.0 6 26 172 17 0.0 6 17 173 51 0.0 6 48 3 174 55 0.0 6 51 4 175 85 0.0 6 84 1 176 50 0.0 6 49 1 177 37 0.0 6 37 178 37 0.0 6 35 2 179 30 0.0 6 28 2 180 33 0.0 6 32 1 181 26 0.0 6 26 182 43 0.0 6 42 1 183 59 0.0 6 56 3 184 74 0.0 6 73 1 185 70 0.0 6 70 186 87 0.0 6 86 0 0 0 0 1 187 71 0.0 6 70 1 188 79 0.0 6 76 3 189 87 0.0 6 84 3 190 66 0.0 6 63 3 191 160 0.0 6 158 2 192 131 0.0 6 124 7 193 120 0.0 6 117 3 194 116 0.0 6 113 3 195 106 0.0 6 101 5 196 158 0.0 6 153 5 197 148 0.0 6 143 5 198 154 0.0 6 149 5 199 197 0.0 6 189 8 200 145 0.0 6 142 3 201 178 0.0 6 169 9 202 184 0.0 6 175 9 203 221 0.0 6 216 4 1 204 190 0.0 6 183 6 1 205 227 0.0 6 217 10 206 172 0.0 6 168 3 1 207 234 0.0 6 231 3 208 228 0.0 6 223 5 209 155 0.0 6 152 2 0 1 210 160 0.0 6 159 1 211 126 0.0 6 122 4 212 105 0.0 6 102 2 1 213 151 0.0 6 148 3 214 137 0.0 6 135 2 215 140 0.0 6 136 2 2 216 141 0.0 6 132 9 217 300 0.0 6 292 8 218 127 0.0 6 125 2 219 247 0.0 6 240 7 220 454 0.0 6 446 7 1 221 216 0.0 6 207 9 222 222 0.0 6 221 1 223 348 0.0 6 340 8 224 208 0.0 6 193 15 225 659 0.0 6 642 17 226 353 0.0 6 340 13 227 305 0.0 6 294 10 1 228 463 0.0 6 452 11 229 240 0.0 6 235 4 1 230 572 0.0 6 562 8 2 231 250 0.0 6 241 9 232 332 0.0 6 325 6 1 233 265 0.0 6 259 6 234 292 0.0 6 283 9 235 201 0.0 6 195 6 236 185 0.0 6 183 2 237 138 0.0 6 128 10 238 185 0.0 6 181 4 239 202 0.0 6 199 3 240 234 0.0 6 229 4 1 241 359 0.0 6 351 8 242 231 0.0 6 225 6 243 428 0.0 6 418 9 1 244 391 0.0 6 376 15 245 449 0.0 6 441 8 246 269 0.0 6 261 8 247 426 0.0 6 424 2 248 249 0.0 6 237 11 0 1 249 414 0.0 6 406 7 0 0 0 1 250 366 0.0 6 351 14 1 251 452 0.0 6 445 7 252 352 0.0 6 344 8 253 369 0.0 6 361 8 254 275 0.0 6 271 4 255 452 0.0 6 438 14 256 635 0.0 6 621 13 0 0 1 257 360 0.0 6 347 12 1 258 336 0.0 6 324 11 1 259 306 0.0 6 296 10 260 489 0.0 6 476 13 261 416 0.0 6 396 20 262 276 0.0 6 267 9 263 377 0.0 6 363 14 264 357 0.0 6 345 10 2 265 436 0.0 6 427 9 266 358 0.0 6 353 4 1 267 330 0.0 6 321 9 268 361 0.0 6 343 18 269 556 0.0 6 543 11 1 1 270 534 0.0 6 520 14 271 592 0.0 6 562 30 272 660 0.0 6 638 21 0 1 273 397 0.0 6 382 15 274 484 0.0 6 472 12 275 634 0.0 6 616 16 2 276 787 0.0 6 770 15 1 0 1 277 1188 0.0 6 1165 22 0 1 278 958 0.0 6 932 25 1 279 1419 0.0 6 1380 37 2 280 5237 0.0 6 5074 152 9 1 1 281 25215 0.0 6 24779 422 13 1 282 53 0.0 6 48 5 283 6 0.0 6 4 1 1 285 1 0.0 6 1 286 1 0.0 6 1 290 1 0.0 6 1 291 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 19 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 1 0.0 6 1 71 1 0.0 6 1 73 1 0.0 6 1 83 1 0.0 6 1 84 1 0.0 6 1 85 1 0.0 6 1 86 1 0.0 6 1 87 1 0.0 6 1 88 1 0.0 6 1 91 1 0.0 6 1 92 1 0.0 6 1 102 1 0.0 6 1 109 1 0.0 6 1 110 1 0.0 6 1 249 5 0.0 6 5 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 62939 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 984.4 0 0 1 6 1 15.4 1 1 10 7 0.1 3 6 1 11 5 0.0 3 4 1 13 8 0.0 3 7 1 14 9 0.0 4 9 16 11 0.0 4 10 1 17 17 0.0 5 13 4 18 8 0.0 5 8 19 6 0.0 5 6 20 59 0.0 6 57 2 21 47 0.0 6 45 2 22 39 0.0 6 38 1 23 16 0.0 6 16 24 30 0.0 6 26 4 25 18 0.0 6 17 0 1 26 13 0.0 6 10 3 27 18 0.0 6 18 28 15 0.0 6 13 2 29 13 0.0 6 13 30 13 0.0 6 11 2 31 16 0.0 6 15 1 32 15 0.0 6 15 33 6 0.0 6 5 0 1 34 14 0.0 6 12 2 35 6 0.0 6 6 36 7 0.0 6 7 37 17 0.0 6 17 38 9 0.0 6 9 39 7 0.0 6 7 40 11 0.0 6 11 41 11 0.0 6 10 1 42 6 0.0 6 5 1 43 11 0.0 6 10 0 1 44 14 0.0 6 12 2 45 3 0.0 6 2 1 46 20 0.0 6 16 4 47 25 0.0 6 23 2 48 9 0.0 6 7 2 49 17 0.0 6 16 1 50 10 0.0 6 9 1 51 11 0.0 6 10 1 52 22 0.0 6 20 2 53 14 0.0 6 10 3 1 54 25 0.0 6 23 2 55 15 0.0 6 13 0 1 0 1 56 28 0.0 6 19 6 2 0 1 57 26 0.0 6 22 4 58 16 0.0 6 15 1 59 7 0.0 6 6 1 60 18 0.0 6 16 0 0 0 2 61 10 0.0 6 8 1 0 0 0 0 1 62 11 0.0 6 10 0 1 63 13 0.0 6 10 3 64 15 0.0 6 10 3 1 1 65 10 0.0 6 9 0 0 0 1 66 21 0.0 6 21 67 129 0.0 6 127 2 68 34 0.0 6 33 1 69 35 0.0 6 34 1 70 13 0.0 6 13 71 36 0.0 6 34 2 72 22 0.0 6 21 1 73 10 0.0 6 9 1 74 12 0.0 6 11 1 75 19 0.0 6 17 2 76 21 0.0 6 19 2 77 17 0.0 6 14 3 78 29 0.0 6 27 2 79 31 0.0 6 29 2 80 23 0.0 6 19 4 81 17 0.0 6 17 82 27 0.0 6 22 5 83 48 0.0 6 43 5 84 39 0.0 6 36 1 1 1 85 24 0.0 6 19 4 1 86 22 0.0 6 20 1 1 87 35 0.0 6 34 1 88 21 0.0 6 19 2 89 24 0.0 6 22 2 90 26 0.0 6 24 1 1 91 32 0.0 6 26 6 92 21 0.0 6 21 93 38 0.0 6 35 2 1 94 68 0.0 6 61 6 0 1 95 29 0.0 6 27 2 96 28 0.0 6 24 4 97 37 0.0 6 31 5 0 0 1 98 35 0.0 6 30 5 99 35 0.0 6 31 4 100 47 0.0 6 45 2 101 52 0.0 6 47 5 102 68 0.0 6 67 1 103 73 0.0 6 71 2 104 55 0.0 6 51 4 105 42 0.0 6 38 4 106 31 0.0 6 30 1 107 38 0.0 6 38 108 39 0.0 6 38 1 109 45 0.0 6 43 2 110 49 0.0 6 45 4 111 65 0.0 6 63 1 1 112 50 0.0 6 49 1 113 48 0.0 6 43 5 114 62 0.0 6 57 4 1 115 43 0.0 6 41 2 116 52 0.0 6 47 3 1 1 117 35 0.0 6 31 3 1 118 54 0.0 6 47 6 0 1 119 76 0.0 6 71 4 1 120 121 0.0 6 112 7 2 121 66 0.0 6 58 8 122 113 0.0 6 107 5 1 123 113 0.0 6 106 7 124 145 0.0 6 133 11 1 125 137 0.0 6 127 10 126 78 0.0 6 70 8 127 87 0.0 6 82 4 0 0 1 128 47 0.0 6 45 2 129 51 0.0 6 49 2 130 89 0.0 6 85 4 131 140 0.0 6 132 6 1 0 1 132 123 0.0 6 117 5 1 133 116 0.0 6 107 9 134 239 0.0 6 227 11 1 135 279 0.0 6 269 10 136 198 0.0 6 191 7 137 195 0.0 6 187 8 138 163 0.0 6 153 9 1 139 224 0.0 6 218 5 1 140 243 0.0 6 230 12 0 1 141 230 0.0 6 225 3 0 0 2 142 258 0.0 6 250 8 143 317 0.0 6 305 11 1 144 339 0.0 6 325 14 145 172 0.0 6 163 7 1 0 1 146 171 0.0 6 164 7 147 142 0.0 6 133 8 1 148 292 0.0 6 282 8 2 149 405 0.0 6 389 16 150 555 0.0 6 531 21 0 0 1 2 151 280 0.0 6 271 7 2 152 585 0.0 6 564 21 153 398 0.0 6 383 14 1 154 335 0.0 6 324 11 155 376 0.0 6 362 14 156 426 0.0 6 418 8 157 550 0.0 6 536 14 158 368 0.0 6 356 12 159 360 0.0 6 342 16 0 1 1 160 417 0.0 6 399 16 2 161 242 0.0 6 236 5 1 162 251 0.0 6 241 10 163 432 0.0 6 424 8 164 379 0.0 6 361 17 0 0 1 165 518 0.0 6 506 11 1 166 443 0.0 6 432 11 167 458 0.0 6 442 16 168 603 0.0 6 591 11 1 169 286 0.0 6 274 11 0 0 1 170 438 0.0 6 425 12 0 0 1 171 571 0.0 6 551 18 2 172 381 0.0 6 373 7 1 173 613 0.0 6 594 17 1 0 1 174 548 0.0 6 528 18 1 0 1 175 812 0.0 6 790 21 1 176 711 0.0 6 681 28 2 177 682 0.0 6 661 19 0 0 2 178 714 0.0 6 689 24 1 179 737 0.0 6 707 29 0 1 180 1216 0.0 6 1182 32 2 181 738 0.0 6 719 16 2 0 0 0 1 182 491 0.0 6 468 23 183 1494 0.0 6 1452 41 0 0 1 184 1075 0.0 6 1036 37 1 0 1 185 1360 0.0 6 1316 41 2 0 1 186 1207 0.0 6 1179 26 1 0 1 187 1249 0.0 6 1202 45 0 0 2 188 1138 0.0 6 1105 32 1 189 815 0.0 6 789 25 1 190 1251 0.0 6 1208 36 2 2 3 191 1485 0.0 6 1454 30 1 192 791 0.0 6 769 22 193 511 0.0 6 484 27 194 882 0.0 6 862 17 2 0 1 195 1049 0.0 6 1000 49 196 2186 0.0 6 2131 51 3 0 1 197 791 0.0 6 764 25 2 198 1378 0.0 6 1332 45 1 199 2179 0.0 6 2124 51 2 0 2 200 934 0.0 6 911 22 0 0 0 1 201 1492 0.0 6 1454 35 2 0 1 202 741 0.0 6 721 18 0 1 1 203 784 0.0 6 764 20 204 977 0.0 6 958 19 205 671 0.0 6 655 14 2 206 433 0.0 6 423 9 0 0 1 207 377 0.0 6 365 10 1 0 1 208 513 0.0 6 503 9 0 1 209 376 0.0 6 369 7 210 301 0.0 6 293 8 211 294 0.0 6 286 8 212 439 0.0 6 429 8 1 0 1 213 250 0.0 6 242 8 214 600 0.0 6 576 23 0 0 0 1 215 584 0.0 6 561 22 1 216 537 0.0 6 520 16 1 217 396 0.0 6 392 4 218 273 0.0 6 265 8 219 355 0.0 6 347 7 1 220 149 0.0 6 145 3 0 0 0 1 221 399 0.0 6 392 6 1 222 424 0.0 6 414 10 223 516 0.0 6 507 9 224 295 0.0 6 289 6 225 444 0.0 6 436 7 1 226 262 0.0 6 257 5 227 313 0.0 6 308 5 228 226 0.0 6 218 7 0 1 229 393 0.0 6 377 15 0 0 1 230 137 0.0 6 133 4 231 240 0.0 6 236 4 232 297 0.0 6 286 11 233 183 0.0 6 179 4 234 120 0.0 6 118 2 235 74 0.0 6 72 2 236 25 0.0 6 24 1 237 36 0.0 6 34 2 238 38 0.0 6 38 239 43 0.0 6 41 2 240 96 0.0 6 95 1 241 127 0.0 6 121 5 1 242 131 0.0 6 129 2 243 145 0.0 6 144 1 244 85 0.0 6 83 1 1 245 32 0.0 6 30 2 246 72 0.0 6 68 3 0 1 247 15 0.0 6 13 2 248 65 0.0 6 63 1 0 0 1 249 22 0.0 6 22 250 13 0.0 6 13 251 31 0.0 6 31 252 6 0.0 6 5 1 253 2 0.0 6 2 257 2 0.0 6 2 278 42 0.0 6 39 3 279 142 0.0 6 138 4 280 10 0.0 6 9 1 281 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 20 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 2 0.0 6 2 73 1 0.0 6 1 76 1 0.0 6 1 86 1 0.0 6 1 87 2 0.0 6 2 104 2 0.0 6 2 133 1 0.0 6 1 138 2 0.0 6 2 144 1 0.0 6 1 149 1 0.0 6 1 159 1 0.0 6 1 182 3 0.0 6 3 190 1 0.0 6 1 224 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_16_43_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_16_270_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_16_43_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_16_270_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.47 s (31 µs/read; 1.93 M reads/minute). === Summary === Total read pairs processed: 79,450 Read 1 with adapter: 79,444 (100.0%) Read 2 with adapter: 79,405 (99.9%) == Read fate breakdown == Pairs that were too short: 11 (0.0%) Pairs discarded as untrimmed: 50 (0.1%) Pairs written (passing filters): 79,389 (99.9%) Total basepairs processed: 36,916,391 bp Read 1: 20,772,761 bp Read 2: 16,143,630 bp Total written (filtered): 4,874,580 bp (13.2%) Read 1: 2,454,023 bp Read 2: 2,420,557 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 78194 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 3 310.4 1 0 3 8 1 1.2 2 0 1 9 1 0.3 2 1 10 2 0.1 3 2 15 3 0.0 4 3 16 1 0.0 4 0 1 20 80 0.0 6 75 5 21 36 0.0 6 32 4 22 40 0.0 6 40 23 29 0.0 6 29 24 12 0.0 6 12 25 24 0.0 6 21 1 0 1 0 1 26 13 0.0 6 13 27 12 0.0 6 11 1 28 4 0.0 6 4 29 4 0.0 6 3 0 0 0 0 0 1 30 3 0.0 6 3 31 2 0.0 6 2 32 7 0.0 6 7 33 4 0.0 6 4 34 5 0.0 6 4 1 36 6 0.0 6 6 37 3 0.0 6 3 38 2 0.0 6 2 39 3 0.0 6 3 40 6 0.0 6 4 0 0 2 41 5 0.0 6 5 42 5 0.0 6 5 43 4 0.0 6 3 1 44 6 0.0 6 6 45 5 0.0 6 3 1 1 46 6 0.0 6 6 47 2 0.0 6 2 49 2 0.0 6 2 50 4 0.0 6 4 51 5 0.0 6 5 52 8 0.0 6 7 0 1 53 5 0.0 6 5 54 5 0.0 6 5 55 5 0.0 6 5 56 2 0.0 6 2 57 2 0.0 6 1 1 58 5 0.0 6 4 1 59 2 0.0 6 2 60 3 0.0 6 3 61 4 0.0 6 4 62 4 0.0 6 3 1 63 5 0.0 6 5 64 5 0.0 6 5 65 3 0.0 6 3 66 7 0.0 6 7 67 1 0.0 6 1 68 3 0.0 6 2 1 69 2 0.0 6 2 70 4 0.0 6 3 1 71 2 0.0 6 2 72 4 0.0 6 4 73 3 0.0 6 2 0 0 1 74 2 0.0 6 2 75 7 0.0 6 7 76 5 0.0 6 4 1 77 7 0.0 6 7 78 10 0.0 6 10 79 5 0.0 6 5 80 4 0.0 6 4 81 6 0.0 6 6 82 4 0.0 6 4 83 5 0.0 6 5 84 4 0.0 6 4 85 4 0.0 6 4 86 9 0.0 6 9 87 5 0.0 6 5 88 4 0.0 6 4 89 5 0.0 6 5 90 6 0.0 6 6 91 7 0.0 6 6 1 92 5 0.0 6 5 93 3 0.0 6 3 94 5 0.0 6 5 95 2 0.0 6 2 96 4 0.0 6 4 97 8 0.0 6 8 98 6 0.0 6 6 99 7 0.0 6 7 100 10 0.0 6 10 101 6 0.0 6 5 1 102 7 0.0 6 6 1 103 10 0.0 6 9 1 104 9 0.0 6 9 105 15 0.0 6 15 106 10 0.0 6 8 2 107 15 0.0 6 14 1 108 4 0.0 6 4 109 13 0.0 6 13 110 11 0.0 6 11 111 12 0.0 6 12 112 14 0.0 6 13 0 0 1 113 13 0.0 6 12 1 114 18 0.0 6 17 1 115 14 0.0 6 14 116 10 0.0 6 10 117 20 0.0 6 19 0 1 118 15 0.0 6 15 119 12 0.0 6 12 120 17 0.0 6 17 121 20 0.0 6 20 122 17 0.0 6 17 123 21 0.0 6 20 1 124 27 0.0 6 27 125 14 0.0 6 13 1 126 13 0.0 6 13 127 25 0.0 6 24 1 128 19 0.0 6 17 2 129 27 0.0 6 26 1 130 17 0.0 6 16 1 131 21 0.0 6 21 132 24 0.0 6 24 133 27 0.0 6 25 2 134 31 0.0 6 31 135 37 0.0 6 37 136 28 0.0 6 27 1 137 50 0.0 6 50 138 47 0.0 6 47 139 34 0.0 6 34 140 64 0.0 6 64 141 86 0.0 6 86 142 343 0.0 6 341 2 143 515 0.0 6 513 2 144 442 0.0 6 441 0 0 1 145 194 0.0 6 193 1 146 432 0.0 6 431 1 147 731 0.0 6 730 1 148 285 0.0 6 283 2 149 291 0.0 6 290 1 150 239 0.0 6 236 2 1 151 505 0.0 6 501 4 152 158 0.0 6 157 1 153 169 0.0 6 168 1 154 140 0.0 6 138 2 155 97 0.0 6 95 2 156 151 0.0 6 147 3 0 1 157 480 0.0 6 476 3 1 158 429 0.0 6 428 1 159 212 0.0 6 210 2 160 188 0.0 6 186 2 161 363 0.0 6 363 162 140 0.0 6 139 1 163 223 0.0 6 223 164 183 0.0 6 182 1 165 128 0.0 6 128 166 369 0.0 6 369 167 600 0.0 6 598 2 168 199 0.0 6 199 169 367 0.0 6 364 3 170 260 0.0 6 260 171 196 0.0 6 194 2 172 519 0.0 6 516 2 1 173 454 0.0 6 454 174 208 0.0 6 208 175 424 0.0 6 420 4 176 182 0.0 6 181 1 177 184 0.0 6 180 4 178 245 0.0 6 244 1 179 255 0.0 6 255 180 159 0.0 6 156 3 181 291 0.0 6 290 1 182 151 0.0 6 149 1 1 183 197 0.0 6 194 3 184 292 0.0 6 291 1 185 189 0.0 6 185 4 186 438 0.0 6 430 8 187 199 0.0 6 197 2 188 198 0.0 6 196 1 1 189 434 0.0 6 433 1 190 214 0.0 6 214 191 277 0.0 6 272 5 192 278 0.0 6 276 2 193 273 0.0 6 270 3 194 424 0.0 6 419 4 0 0 0 1 195 303 0.0 6 297 6 196 356 0.0 6 350 5 0 1 197 967 0.0 6 959 6 0 0 1 0 1 198 648 0.0 6 642 6 199 1072 0.0 6 1066 6 200 481 0.0 6 475 6 201 518 0.0 6 513 5 202 301 0.0 6 295 6 203 423 0.0 6 418 5 204 407 0.0 6 400 7 205 380 0.0 6 373 5 2 206 287 0.0 6 280 6 1 207 415 0.0 6 408 7 208 685 0.0 6 683 2 209 647 0.0 6 642 4 1 210 708 0.0 6 703 4 0 0 1 211 324 0.0 6 319 4 1 212 505 0.0 6 501 4 213 360 0.0 6 352 8 214 361 0.0 6 355 5 1 215 458 0.0 6 452 6 216 379 0.0 6 368 11 217 625 0.0 6 610 12 3 218 522 0.0 6 513 8 1 219 409 0.0 6 400 8 0 0 0 1 220 411 0.0 6 404 6 1 221 524 0.0 6 515 8 0 0 0 1 222 411 0.0 6 404 7 223 359 0.0 6 353 6 224 453 0.0 6 447 4 2 225 409 0.0 6 393 15 0 0 0 0 1 226 673 0.0 6 667 5 1 227 612 0.0 6 597 14 0 1 228 626 0.0 6 617 9 229 426 0.0 6 421 5 230 488 0.0 6 478 10 231 520 0.0 6 510 10 232 457 0.0 6 447 9 1 233 498 0.0 6 483 15 234 539 0.0 6 530 9 235 403 0.0 6 397 6 236 434 0.0 6 425 8 1 237 292 0.0 6 283 8 0 0 0 0 1 238 396 0.0 6 388 6 1 0 0 1 239 337 0.0 6 330 7 240 350 0.0 6 343 7 241 441 0.0 6 424 17 242 417 0.0 6 405 12 243 419 0.0 6 412 7 244 524 0.0 6 515 9 245 424 0.0 6 410 13 0 0 1 246 221 0.0 6 218 3 247 285 0.0 6 275 9 0 0 1 248 324 0.0 6 319 4 1 249 370 0.0 6 358 12 250 354 0.0 6 350 3 1 251 391 0.0 6 381 9 1 252 310 0.0 6 299 11 253 306 0.0 6 298 8 254 435 0.0 6 426 9 255 334 0.0 6 316 17 0 0 1 256 302 0.0 6 290 12 257 320 0.0 6 311 9 258 310 0.0 6 299 10 1 259 498 0.0 6 475 23 260 583 0.0 6 566 15 2 261 343 0.0 6 330 11 1 0 0 0 1 262 484 0.0 6 466 18 263 552 0.0 6 526 25 1 264 418 0.0 6 404 14 265 448 0.0 6 434 14 266 294 0.0 6 284 8 2 267 495 0.0 6 484 10 0 0 0 0 1 268 470 0.0 6 445 22 3 269 413 0.0 6 402 11 270 252 0.0 6 244 7 0 1 271 693 0.0 6 660 31 2 272 894 0.0 6 856 36 2 273 500 0.0 6 484 16 274 582 0.0 6 563 19 275 766 0.0 6 739 25 2 276 1114 0.0 6 1082 29 3 277 1008 0.0 6 980 28 278 895 0.0 6 866 27 2 279 1162 0.0 6 1128 33 1 280 1863 0.0 6 1786 71 4 2 281 17810 0.0 6 17430 373 7 282 14 0.0 6 12 2 283 2 0.0 6 2 284 2 0.0 6 1 0 0 1 285 1 0.0 6 1 286 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1250 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 3 0.0 6 2 1 61 178 0.0 6 175 3 67 1 0.0 6 0 0 0 0 0 0 1 68 1 0.0 6 1 70 4 0.0 6 4 71 12 0.0 6 12 72 12 0.0 6 12 73 16 0.0 6 15 1 74 5 0.0 6 5 75 15 0.0 6 15 76 26 0.0 6 26 77 7 0.0 6 7 78 6 0.0 6 6 79 16 0.0 6 16 80 11 0.0 6 11 81 4 0.0 6 4 82 4 0.0 6 4 83 3 0.0 6 3 84 1 0.0 6 1 85 4 0.0 6 4 86 25 0.0 6 25 87 18 0.0 6 18 88 5 0.0 6 5 89 7 0.0 6 7 90 17 0.0 6 17 91 1 0.0 6 1 92 3 0.0 6 3 93 2 0.0 6 2 94 7 0.0 6 7 95 15 0.0 6 15 96 17 0.0 6 17 97 7 0.0 6 7 98 11 0.0 6 11 99 10 0.0 6 9 1 100 6 0.0 6 6 101 19 0.0 6 18 1 102 10 0.0 6 10 103 8 0.0 6 8 104 14 0.0 6 14 105 4 0.0 6 4 106 3 0.0 6 3 107 5 0.0 6 5 108 8 0.0 6 8 109 2 0.0 6 2 110 6 0.0 6 6 112 3 0.0 6 3 113 3 0.0 6 3 114 1 0.0 6 1 115 17 0.0 6 17 116 4 0.0 6 4 117 11 0.0 6 11 118 8 0.0 6 8 119 3 0.0 6 3 120 4 0.0 6 4 121 1 0.0 6 1 122 1 0.0 6 1 123 7 0.0 6 7 124 3 0.0 6 3 125 8 0.0 6 8 126 29 0.0 6 29 127 9 0.0 6 9 128 21 0.0 6 21 129 11 0.0 6 11 130 7 0.0 6 7 131 3 0.0 6 3 132 6 0.0 6 6 133 9 0.0 6 9 134 1 0.0 6 1 135 4 0.0 6 4 136 1 0.0 6 1 137 10 0.0 6 10 138 18 0.0 6 18 139 12 0.0 6 12 140 8 0.0 6 8 141 18 0.0 6 18 142 8 0.0 6 8 143 1 0.0 6 1 145 1 0.0 6 1 146 2 0.0 6 2 147 1 0.0 6 1 148 1 0.0 6 1 150 4 0.0 6 4 151 1 0.0 6 1 152 8 0.0 6 8 153 12 0.0 6 12 154 6 0.0 6 6 155 14 0.0 6 14 156 3 0.0 6 3 157 8 0.0 6 8 158 1 0.0 6 1 159 16 0.0 6 16 160 33 0.0 6 33 161 1 0.0 6 1 162 6 0.0 6 6 163 11 0.0 6 11 164 10 0.0 6 10 165 17 0.0 6 17 166 4 0.0 6 4 167 8 0.0 6 8 169 3 0.0 6 3 172 1 0.0 6 1 173 2 0.0 6 2 174 2 0.0 6 2 175 1 0.0 6 1 176 13 0.0 6 13 177 25 0.0 6 25 178 1 0.0 6 1 179 7 0.0 6 7 180 15 0.0 6 15 181 5 0.0 6 5 182 7 0.0 6 7 183 9 0.0 6 9 184 1 0.0 6 1 185 3 0.0 6 3 186 1 0.0 6 1 187 2 0.0 6 2 189 4 0.0 6 4 190 4 0.0 6 4 192 2 0.0 6 2 195 11 0.0 6 11 196 3 0.0 6 3 197 1 0.0 6 1 198 4 0.0 6 4 199 2 0.0 6 2 200 1 0.0 6 1 201 2 0.0 6 2 202 1 0.0 6 1 204 2 0.0 6 2 205 10 0.0 6 10 206 11 0.0 6 11 207 6 0.0 6 6 209 1 0.0 6 1 210 13 0.0 6 13 211 3 0.0 6 3 212 1 0.0 6 1 213 1 0.0 6 1 214 4 0.0 6 4 215 7 0.0 6 7 216 2 0.0 6 2 217 1 0.0 6 1 218 3 0.0 6 3 219 1 0.0 6 1 220 2 0.0 6 2 221 1 0.0 6 1 222 2 0.0 6 2 223 5 0.0 6 5 224 8 0.0 6 8 225 1 0.0 6 1 226 1 0.0 6 1 228 1 0.0 6 1 229 1 0.0 6 1 230 4 0.0 6 4 231 2 0.0 6 2 232 9 0.0 6 9 233 3 0.0 6 3 236 5 0.0 6 5 239 1 0.0 6 1 241 4 0.0 6 4 245 4 0.0 6 4 246 6 0.0 6 6 251 2 0.0 6 2 252 1 0.0 6 1 260 1 0.0 6 1 261 1 0.0 6 1 262 1 0.0 6 1 272 1 0.0 6 1 278 2 0.0 6 2 279 1 0.0 6 1 281 3 0.0 6 3 311 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 78407 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 77.6 1 1 7 2 4.8 2 0 2 9 1 0.3 2 1 11 3 0.0 3 3 12 2 0.0 3 2 13 14 0.0 3 11 3 14 6 0.0 4 6 16 9 0.0 4 7 2 17 6 0.0 5 6 18 7 0.0 5 5 2 19 14 0.0 5 12 2 20 103 0.0 6 99 4 21 48 0.0 6 47 1 22 46 0.0 6 44 2 23 29 0.0 6 28 1 24 21 0.0 6 20 1 25 22 0.0 6 22 26 17 0.0 6 17 27 10 0.0 6 10 28 12 0.0 6 11 1 29 13 0.0 6 12 1 30 10 0.0 6 10 31 17 0.0 6 17 32 8 0.0 6 8 33 11 0.0 6 11 34 7 0.0 6 7 35 10 0.0 6 9 1 36 9 0.0 6 9 37 13 0.0 6 12 1 38 12 0.0 6 12 39 13 0.0 6 13 40 8 0.0 6 7 1 41 6 0.0 6 6 42 9 0.0 6 9 43 10 0.0 6 9 1 44 21 0.0 6 20 1 45 12 0.0 6 10 2 46 7 0.0 6 7 47 19 0.0 6 17 2 48 10 0.0 6 10 49 15 0.0 6 15 50 18 0.0 6 17 1 51 5 0.0 6 5 52 17 0.0 6 16 1 53 12 0.0 6 11 1 54 13 0.0 6 12 0 1 55 22 0.0 6 19 3 56 17 0.0 6 16 1 57 21 0.0 6 16 5 58 14 0.0 6 13 1 59 14 0.0 6 14 60 13 0.0 6 13 61 9 0.0 6 9 62 10 0.0 6 10 63 6 0.0 6 5 0 0 0 1 64 20 0.0 6 19 1 65 16 0.0 6 15 1 66 16 0.0 6 15 1 67 23 0.0 6 21 2 68 47 0.0 6 43 4 69 132 0.0 6 126 6 70 31 0.0 6 29 2 71 69 0.0 6 67 2 72 33 0.0 6 31 2 73 32 0.0 6 28 3 1 74 20 0.0 6 19 0 0 0 1 75 27 0.0 6 24 3 76 29 0.0 6 27 2 77 26 0.0 6 23 3 78 31 0.0 6 30 1 79 26 0.0 6 26 80 17 0.0 6 15 1 0 0 1 81 35 0.0 6 33 2 82 40 0.0 6 39 1 83 37 0.0 6 32 5 84 39 0.0 6 37 2 85 63 0.0 6 60 3 86 28 0.0 6 28 87 34 0.0 6 33 0 0 1 88 27 0.0 6 27 89 42 0.0 6 40 2 90 36 0.0 6 33 3 91 38 0.0 6 35 3 92 32 0.0 6 32 93 54 0.0 6 53 1 94 79 0.0 6 73 5 1 95 43 0.0 6 38 5 96 54 0.0 6 51 3 97 55 0.0 6 50 5 98 57 0.0 6 53 4 99 55 0.0 6 51 2 1 1 100 71 0.0 6 69 2 101 47 0.0 6 44 3 102 87 0.0 6 81 6 103 77 0.0 6 73 4 104 93 0.0 6 90 1 2 105 77 0.0 6 75 2 106 100 0.0 6 96 2 0 0 2 107 83 0.0 6 81 2 108 54 0.0 6 49 5 109 73 0.0 6 72 1 110 62 0.0 6 60 1 1 111 75 0.0 6 72 2 1 112 80 0.0 6 77 2 1 113 79 0.0 6 77 2 114 106 0.0 6 102 3 0 1 115 86 0.0 6 83 3 116 91 0.0 6 88 3 117 92 0.0 6 89 3 118 100 0.0 6 95 5 119 131 0.0 6 125 3 1 1 1 120 154 0.0 6 147 4 3 121 117 0.0 6 112 5 122 133 0.0 6 129 4 123 148 0.0 6 141 7 124 160 0.0 6 156 4 125 132 0.0 6 128 3 0 0 0 1 126 126 0.0 6 124 2 127 188 0.0 6 180 6 1 0 1 128 173 0.0 6 167 5 1 129 270 0.0 6 264 5 0 1 130 203 0.0 6 197 5 0 0 1 131 262 0.0 6 256 5 0 0 1 132 256 0.0 6 247 7 0 1 1 133 294 0.0 6 283 10 0 0 0 1 134 275 0.0 6 267 6 1 0 1 135 405 0.0 6 395 8 2 136 311 0.0 6 304 7 137 405 0.0 6 396 8 1 138 419 0.0 6 417 1 0 0 1 139 550 0.0 6 540 10 140 690 0.0 6 676 10 2 1 0 1 141 631 0.0 6 620 11 142 603 0.0 6 594 7 0 0 1 1 143 747 0.0 6 736 11 144 1105 0.0 6 1094 10 1 145 547 0.0 6 538 8 0 0 1 146 596 0.0 6 585 11 147 524 0.0 6 513 10 1 148 710 0.0 6 703 7 149 647 0.0 6 634 12 0 1 150 809 0.0 6 790 19 151 636 0.0 6 626 7 1 2 152 1042 0.0 6 1022 16 1 1 2 153 732 0.0 6 712 19 1 154 1162 0.0 6 1142 18 1 0 1 155 1060 0.0 6 1048 10 1 0 1 156 764 0.0 6 745 17 1 0 0 0 1 157 911 0.0 6 884 26 0 1 158 975 0.0 6 966 8 0 0 1 159 580 0.0 6 574 5 0 1 160 876 0.0 6 855 21 161 692 0.0 6 679 12 1 162 702 0.0 6 694 8 163 879 0.0 6 862 12 2 0 1 0 2 164 887 0.0 6 873 13 0 0 1 165 734 0.0 6 726 8 166 886 0.0 6 874 10 0 0 2 167 573 0.0 6 564 8 0 0 1 168 1087 0.0 6 1060 26 0 0 1 169 718 0.0 6 705 12 0 0 1 170 850 0.0 6 831 14 2 1 1 1 171 953 0.0 6 935 17 0 0 1 172 641 0.0 6 633 8 173 637 0.0 6 622 15 174 884 0.0 6 861 21 2 175 1150 0.0 6 1108 39 1 0 2 176 884 0.0 6 866 16 2 177 1016 0.0 6 996 19 1 178 827 0.0 6 809 17 0 0 1 179 868 0.0 6 848 19 1 180 835 0.0 6 805 29 0 1 181 912 0.0 6 884 27 1 182 407 0.0 6 398 7 2 183 806 0.0 6 783 21 0 0 2 184 410 0.0 6 399 10 0 0 0 0 1 185 630 0.0 6 620 8 2 186 1734 0.0 6 1685 43 3 1 2 187 1353 0.0 6 1313 34 1 1 3 0 1 188 1229 0.0 6 1190 37 1 1 189 1153 0.0 6 1108 41 2 1 0 1 190 1664 0.0 6 1613 50 1 191 1446 0.0 6 1401 44 1 192 1045 0.0 6 1020 19 3 1 2 193 772 0.0 6 746 24 0 2 194 1110 0.0 6 1078 28 2 0 1 0 1 195 1396 0.0 6 1349 45 0 1 0 0 1 196 2588 0.0 6 2520 65 1 0 2 197 538 0.0 6 513 21 3 0 1 198 1955 0.0 6 1883 69 1 0 2 199 2850 0.0 6 2763 82 1 1 3 200 699 0.0 6 675 22 2 201 863 0.0 6 840 22 0 0 0 0 1 202 478 0.0 6 462 15 0 0 1 203 552 0.0 6 534 17 0 0 1 204 638 0.0 6 618 20 205 281 0.0 6 270 10 0 0 1 206 260 0.0 6 253 7 207 213 0.0 6 206 6 1 208 311 0.0 6 300 11 209 319 0.0 6 313 4 2 210 296 0.0 6 285 11 211 387 0.0 6 375 12 212 302 0.0 6 295 7 213 100 0.0 6 94 5 0 0 0 1 214 181 0.0 6 179 2 215 235 0.0 6 220 14 0 0 1 216 727 0.0 6 693 33 0 1 217 433 0.0 6 420 13 218 327 0.0 6 319 8 219 277 0.0 6 268 8 0 0 1 220 270 0.0 6 260 10 221 230 0.0 6 221 9 222 447 0.0 6 437 10 223 959 0.0 6 931 27 1 224 182 0.0 6 173 9 225 313 0.0 6 302 11 226 191 0.0 6 184 5 0 0 2 227 168 0.0 6 158 8 0 0 2 228 95 0.0 6 91 4 229 161 0.0 6 154 6 0 0 1 230 99 0.0 6 96 3 231 139 0.0 6 135 4 232 188 0.0 6 184 4 233 256 0.0 6 247 8 1 234 88 0.0 6 84 4 235 36 0.0 6 33 3 236 30 0.0 6 29 1 237 22 0.0 6 21 1 238 10 0.0 6 7 3 239 7 0.0 6 7 240 72 0.0 6 67 5 241 35 0.0 6 33 2 242 86 0.0 6 86 243 121 0.0 6 119 2 244 57 0.0 6 56 1 245 12 0.0 6 12 246 16 0.0 6 16 247 12 0.0 6 12 248 19 0.0 6 19 249 13 0.0 6 13 250 22 0.0 6 22 251 11 0.0 6 11 252 5 0.0 6 5 253 12 0.0 6 12 257 1 0.0 6 1 278 1 0.0 6 1 279 7 0.0 6 7 280 29 0.0 6 28 1 281 166 0.0 6 163 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 998 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 53 9 0.0 6 9 70 8 0.0 6 8 71 15 0.0 6 15 72 16 0.0 6 16 73 22 0.0 6 22 74 6 0.0 6 6 75 21 0.0 6 21 76 27 0.0 6 27 77 13 0.0 6 13 78 10 0.0 6 10 79 7 0.0 6 7 80 18 0.0 6 17 1 81 4 0.0 6 4 82 10 0.0 6 10 83 5 0.0 6 5 84 3 0.0 6 3 85 13 0.0 6 13 86 24 0.0 6 24 87 13 0.0 6 13 88 9 0.0 6 9 89 8 0.0 6 8 90 11 0.0 6 10 0 0 0 1 91 1 0.0 6 1 92 4 0.0 6 4 93 2 0.0 6 2 94 3 0.0 6 3 95 13 0.0 6 13 96 17 0.0 6 17 97 5 0.0 6 5 98 11 0.0 6 11 99 5 0.0 6 5 100 5 0.0 6 5 101 25 0.0 6 25 102 14 0.0 6 14 103 6 0.0 6 6 104 9 0.0 6 9 105 2 0.0 6 2 106 3 0.0 6 3 107 8 0.0 6 7 1 108 9 0.0 6 9 109 1 0.0 6 1 110 11 0.0 6 11 111 1 0.0 6 1 112 3 0.0 6 3 113 5 0.0 6 5 114 6 0.0 6 6 115 10 0.0 6 10 117 6 0.0 6 6 118 18 0.0 6 18 119 4 0.0 6 4 120 2 0.0 6 2 121 3 0.0 6 3 122 2 0.0 6 2 123 5 0.0 6 5 124 2 0.0 6 1 0 0 0 1 125 5 0.0 6 5 126 34 0.0 6 34 127 15 0.0 6 15 128 40 0.0 6 40 129 3 0.0 6 3 130 17 0.0 6 16 0 0 0 1 131 1 0.0 6 1 132 2 0.0 6 2 133 5 0.0 6 5 134 1 0.0 6 1 136 2 0.0 6 2 137 13 0.0 6 13 138 14 0.0 6 14 139 28 0.0 6 28 140 7 0.0 6 7 141 19 0.0 6 19 142 4 0.0 6 4 144 4 0.0 6 4 145 1 0.0 6 1 146 6 0.0 6 6 147 1 0.0 6 1 150 6 0.0 6 6 151 2 0.0 6 2 152 5 0.0 6 5 153 13 0.0 6 13 154 8 0.0 6 8 155 18 0.0 6 18 156 1 0.0 6 1 157 8 0.0 6 8 159 11 0.0 6 11 160 28 0.0 6 28 161 4 0.0 6 4 162 11 0.0 6 11 163 8 0.0 6 8 164 5 0.0 6 4 0 0 0 1 165 15 0.0 6 15 166 3 0.0 6 3 167 8 0.0 6 8 168 1 0.0 6 1 169 1 0.0 6 1 171 1 0.0 6 1 172 1 0.0 6 1 173 1 0.0 6 1 174 2 0.0 6 2 175 3 0.0 6 3 176 5 0.0 6 4 0 0 0 1 177 14 0.0 6 14 179 5 0.0 6 5 180 18 0.0 6 18 181 1 0.0 6 1 182 3 0.0 6 3 183 6 0.0 6 6 184 3 0.0 6 3 185 5 0.0 6 5 186 1 0.0 6 1 187 3 0.0 6 3 188 1 0.0 6 1 189 1 0.0 6 1 190 7 0.0 6 7 192 1 0.0 6 1 193 1 0.0 6 1 194 1 0.0 6 1 195 2 0.0 6 2 196 1 0.0 6 1 197 1 0.0 6 1 198 1 0.0 6 1 200 1 0.0 6 1 201 1 0.0 6 1 202 2 0.0 6 1 0 0 0 1 205 3 0.0 6 3 206 12 0.0 6 12 207 2 0.0 6 2 209 1 0.0 6 1 210 4 0.0 6 4 213 1 0.0 6 1 214 2 0.0 6 2 215 3 0.0 6 3 217 1 0.0 6 1 220 1 0.0 6 1 222 2 0.0 6 2 223 1 0.0 6 1 236 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_17_44_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_17_271_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_17_44_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_17_271_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.81 s (174 µs/read; 0.34 M reads/minute). === Summary === Total read pairs processed: 4,635 Read 1 with adapter: 4,626 (99.8%) Read 2 with adapter: 4,626 (99.8%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 9 (0.2%) Pairs written (passing filters): 4,626 (99.8%) Total basepairs processed: 1,569,906 bp Read 1: 797,413 bp Read 2: 772,493 bp Total written (filtered): 294,119 bp (18.7%) Read 1: 150,442 bp Read 2: 143,677 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 4453 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 72.4 0 0 1 20 10 0.0 6 10 21 8 0.0 6 7 1 22 10 0.0 6 8 2 23 4 0.0 6 4 24 1 0.0 6 1 25 1 0.0 6 1 28 2 0.0 6 1 0 0 0 0 0 1 29 2 0.0 6 2 33 1 0.0 6 0 1 35 1 0.0 6 1 43 2 0.0 6 2 44 1 0.0 6 1 45 1 0.0 6 1 47 1 0.0 6 1 51 1 0.0 6 1 52 1 0.0 6 1 62 1 0.0 6 1 64 3 0.0 6 3 66 1 0.0 6 1 72 1 0.0 6 1 73 3 0.0 6 3 74 1 0.0 6 1 75 4 0.0 6 4 76 4 0.0 6 4 77 2 0.0 6 2 84 2 0.0 6 2 94 3 0.0 6 3 96 2 0.0 6 2 97 2 0.0 6 2 99 2 0.0 6 1 1 100 1 0.0 6 1 101 1 0.0 6 1 102 2 0.0 6 2 103 3 0.0 6 3 104 8 0.0 6 8 105 5 0.0 6 5 106 5 0.0 6 5 107 11 0.0 6 11 108 12 0.0 6 12 109 4 0.0 6 4 111 2 0.0 6 2 112 1 0.0 6 1 113 6 0.0 6 6 114 10 0.0 6 10 115 1 0.0 6 1 116 2 0.0 6 2 117 5 0.0 6 5 118 3 0.0 6 3 119 1 0.0 6 1 120 1 0.0 6 1 121 5 0.0 6 5 122 4 0.0 6 4 123 4 0.0 6 4 124 5 0.0 6 5 125 5 0.0 6 5 126 7 0.0 6 7 127 3 0.0 6 3 128 9 0.0 6 9 129 8 0.0 6 8 130 11 0.0 6 11 131 18 0.0 6 17 1 132 8 0.0 6 8 133 4 0.0 6 4 134 5 0.0 6 5 135 8 0.0 6 7 1 136 8 0.0 6 8 137 18 0.0 6 17 1 138 15 0.0 6 15 139 57 0.0 6 57 140 526 0.0 6 525 1 141 1100 0.0 6 1098 2 142 155 0.0 6 154 1 143 136 0.0 6 135 0 0 0 0 0 1 144 39 0.0 6 39 145 75 0.0 6 74 0 0 0 0 0 1 146 468 0.0 6 466 2 147 1221 0.0 6 1221 148 140 0.0 6 140 149 6 0.0 6 6 150 26 0.0 6 26 151 7 0.0 6 7 153 1 0.0 6 1 154 1 0.0 6 1 157 2 0.0 6 2 159 2 0.0 6 2 160 1 0.0 6 1 161 3 0.0 6 3 162 3 0.0 6 3 163 2 0.0 6 2 164 3 0.0 6 3 165 2 0.0 6 2 166 14 0.0 6 14 167 127 0.0 6 127 168 7 0.0 6 7 172 1 0.0 6 1 173 2 0.0 6 2 174 9 0.0 6 9 175 1 0.0 6 1 176 3 0.0 6 3 208 1 0.0 6 1 210 1 0.0 6 1 270 1 0.0 6 1 279 1 0.0 6 1 281 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 173 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 10 0.0 6 10 70 19 0.0 6 19 71 50 0.0 6 50 72 2 0.0 6 2 73 3 0.0 6 3 75 4 0.0 6 4 76 37 0.0 6 37 77 41 0.0 6 40 1 78 1 0.0 6 1 81 1 0.0 6 1 95 1 0.0 6 1 96 2 0.0 6 2 97 1 0.0 6 1 105 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 4468 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 12 1 0.0 3 1 16 1 0.0 4 1 17 1 0.0 5 1 20 3 0.0 6 3 24 1 0.0 6 0 0 0 1 26 2 0.0 6 2 27 1 0.0 6 1 30 2 0.0 6 2 34 1 0.0 6 1 35 1 0.0 6 1 37 1 0.0 6 1 38 2 0.0 6 2 40 1 0.0 6 1 41 4 0.0 6 4 42 1 0.0 6 0 1 44 3 0.0 6 3 46 1 0.0 6 1 48 5 0.0 6 5 49 1 0.0 6 1 50 1 0.0 6 1 53 1 0.0 6 1 54 2 0.0 6 2 56 1 0.0 6 1 58 2 0.0 6 2 61 1 0.0 6 1 63 1 0.0 6 1 66 2 0.0 6 2 67 2 0.0 6 2 69 1 0.0 6 1 70 6 0.0 6 6 71 1 0.0 6 1 72 7 0.0 6 7 73 2 0.0 6 2 74 2 0.0 6 2 76 3 0.0 6 3 77 3 0.0 6 3 78 1 0.0 6 1 79 2 0.0 6 2 81 2 0.0 6 2 82 3 0.0 6 3 83 2 0.0 6 2 84 2 0.0 6 2 85 3 0.0 6 3 86 10 0.0 6 10 87 3 0.0 6 3 88 3 0.0 6 3 89 4 0.0 6 4 90 2 0.0 6 2 91 4 0.0 6 4 92 2 0.0 6 2 93 3 0.0 6 3 94 6 0.0 6 6 95 6 0.0 6 6 96 5 0.0 6 5 97 2 0.0 6 2 98 3 0.0 6 3 99 5 0.0 6 5 100 1 0.0 6 1 101 5 0.0 6 5 102 1 0.0 6 1 103 5 0.0 6 5 104 2 0.0 6 2 105 2 0.0 6 2 106 8 0.0 6 8 107 8 0.0 6 7 1 108 7 0.0 6 7 109 4 0.0 6 4 110 3 0.0 6 3 111 8 0.0 6 8 112 8 0.0 6 8 113 7 0.0 6 7 114 11 0.0 6 11 115 19 0.0 6 18 0 0 1 116 20 0.0 6 20 117 10 0.0 6 10 118 14 0.0 6 14 119 13 0.0 6 13 120 16 0.0 6 16 121 17 0.0 6 17 122 21 0.0 6 21 123 17 0.0 6 17 124 12 0.0 6 12 125 12 0.0 6 12 126 14 0.0 6 14 127 21 0.0 6 21 128 45 0.0 6 45 129 20 0.0 6 19 1 130 12 0.0 6 12 131 7 0.0 6 7 132 17 0.0 6 17 133 15 0.0 6 15 134 36 0.0 6 36 135 51 0.0 6 51 136 123 0.0 6 123 137 197 0.0 6 197 138 585 0.0 6 584 1 139 821 0.0 6 820 1 140 105 0.0 6 105 141 102 0.0 6 102 142 83 0.0 6 82 1 143 148 0.0 6 147 1 144 453 0.0 6 452 1 145 949 0.0 6 948 1 146 69 0.0 6 69 147 10 0.0 6 10 148 26 0.0 6 26 149 10 0.0 6 10 150 4 0.0 6 4 151 1 0.0 6 1 152 3 0.0 6 3 153 3 0.0 6 3 154 2 0.0 6 2 155 3 0.0 6 3 156 3 0.0 6 3 157 2 0.0 6 2 158 5 0.0 6 5 159 4 0.0 6 4 160 11 0.0 6 11 161 7 0.0 6 7 162 6 0.0 6 6 163 18 0.0 6 18 164 15 0.0 6 15 165 51 0.0 6 51 166 1 0.0 6 1 168 1 0.0 6 1 170 2 0.0 6 2 171 2 0.0 6 2 172 5 0.0 6 5 173 1 0.0 6 1 174 2 0.0 6 2 187 1 0.0 6 1 201 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 158 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 50 1 0.0 6 1 66 1 0.0 6 1 68 9 0.0 6 9 69 32 0.0 6 31 1 70 26 0.0 6 26 71 1 0.0 6 1 72 4 0.0 6 4 73 2 0.0 6 2 74 9 0.0 6 6 0 0 0 3 75 22 0.0 6 22 76 44 0.0 6 44 77 2 0.0 6 2 79 1 0.0 6 1 96 3 0.0 6 3 103 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_18_45_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_18_272_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_18_45_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_18_272_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.93 s (39 µs/read; 1.54 M reads/minute). === Summary === Total read pairs processed: 49,496 Read 1 with adapter: 49,494 (100.0%) Read 2 with adapter: 49,456 (99.9%) == Read fate breakdown == Pairs that were too short: 1 (0.0%) Pairs discarded as untrimmed: 42 (0.1%) Pairs written (passing filters): 49,453 (99.9%) Total basepairs processed: 25,494,592 bp Read 1: 14,678,942 bp Read 2: 10,815,650 bp Total written (filtered): 3,070,250 bp (12.0%) Read 1: 1,486,359 bp Read 2: 1,583,891 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 49449 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 773.4 0 0 2 4 2 193.3 1 1 1 6 1 12.1 1 0 0 1 10 1 0.0 3 1 16 1 0.0 4 1 20 11 0.0 6 10 1 21 9 0.0 6 6 3 22 6 0.0 6 5 1 23 7 0.0 6 7 24 3 0.0 6 3 25 3 0.0 6 3 26 4 0.0 6 3 1 27 3 0.0 6 3 28 6 0.0 6 6 29 2 0.0 6 2 30 1 0.0 6 1 31 1 0.0 6 1 32 2 0.0 6 2 34 3 0.0 6 2 1 36 1 0.0 6 0 1 37 1 0.0 6 0 1 38 3 0.0 6 2 1 39 1 0.0 6 1 40 2 0.0 6 2 41 1 0.0 6 0 0 0 0 0 1 42 1 0.0 6 0 1 44 3 0.0 6 3 45 2 0.0 6 2 46 5 0.0 6 5 47 1 0.0 6 1 51 4 0.0 6 3 1 52 1 0.0 6 1 53 1 0.0 6 1 54 2 0.0 6 2 55 1 0.0 6 1 56 4 0.0 6 4 57 3 0.0 6 2 1 58 2 0.0 6 2 60 2 0.0 6 2 62 3 0.0 6 3 63 1 0.0 6 1 66 1 0.0 6 1 67 2 0.0 6 2 69 1 0.0 6 1 70 1 0.0 6 1 72 3 0.0 6 3 74 2 0.0 6 2 75 1 0.0 6 1 76 1 0.0 6 1 77 5 0.0 6 5 78 5 0.0 6 5 79 3 0.0 6 3 82 1 0.0 6 1 83 1 0.0 6 1 86 7 0.0 6 6 1 87 3 0.0 6 3 88 2 0.0 6 2 89 2 0.0 6 2 90 3 0.0 6 3 91 3 0.0 6 3 92 1 0.0 6 0 0 1 93 1 0.0 6 1 96 1 0.0 6 1 97 5 0.0 6 5 98 1 0.0 6 1 100 1 0.0 6 1 101 4 0.0 6 4 102 5 0.0 6 5 103 4 0.0 6 4 104 1 0.0 6 1 105 1 0.0 6 1 106 1 0.0 6 1 107 5 0.0 6 5 108 8 0.0 6 8 109 3 0.0 6 3 110 2 0.0 6 2 111 3 0.0 6 2 1 112 3 0.0 6 2 1 113 4 0.0 6 4 114 4 0.0 6 4 115 5 0.0 6 4 1 116 6 0.0 6 5 1 117 8 0.0 6 8 118 1 0.0 6 0 1 119 2 0.0 6 2 120 5 0.0 6 4 0 1 121 5 0.0 6 5 122 16 0.0 6 15 1 123 7 0.0 6 7 124 11 0.0 6 10 1 125 1 0.0 6 1 126 7 0.0 6 7 127 9 0.0 6 9 128 8 0.0 6 7 1 129 11 0.0 6 11 130 9 0.0 6 9 131 6 0.0 6 5 1 132 17 0.0 6 16 1 133 7 0.0 6 6 0 1 134 12 0.0 6 12 135 9 0.0 6 9 136 7 0.0 6 7 137 9 0.0 6 9 138 13 0.0 6 12 1 139 5 0.0 6 5 140 8 0.0 6 7 1 141 10 0.0 6 10 142 14 0.0 6 14 143 62 0.0 6 62 144 25 0.0 6 25 145 70 0.0 6 70 146 19 0.0 6 19 147 14 0.0 6 14 148 44 0.0 6 44 149 14 0.0 6 14 150 14 0.0 6 14 151 23 0.0 6 23 152 26 0.0 6 25 0 1 153 22 0.0 6 22 154 32 0.0 6 31 1 155 14 0.0 6 13 1 156 32 0.0 6 31 1 157 20 0.0 6 20 158 13 0.0 6 13 159 61 0.0 6 61 160 24 0.0 6 23 1 161 25 0.0 6 23 2 162 23 0.0 6 23 163 57 0.0 6 56 1 164 61 0.0 6 60 1 165 78 0.0 6 77 1 166 26 0.0 6 26 167 23 0.0 6 23 168 14 0.0 6 14 169 12 0.0 6 12 170 43 0.0 6 40 3 171 103 0.0 6 103 172 29 0.0 6 28 1 173 36 0.0 6 33 3 174 49 0.0 6 48 1 175 38 0.0 6 37 1 176 24 0.0 6 24 177 24 0.0 6 24 178 18 0.0 6 18 179 18 0.0 6 17 1 180 23 0.0 6 23 181 38 0.0 6 36 2 182 29 0.0 6 28 1 183 30 0.0 6 29 1 184 53 0.0 6 52 1 185 57 0.0 6 55 2 186 65 0.0 6 64 1 187 39 0.0 6 39 188 76 0.0 6 70 6 189 85 0.0 6 83 2 190 115 0.0 6 109 6 191 105 0.0 6 100 5 192 72 0.0 6 68 4 193 52 0.0 6 49 3 194 53 0.0 6 51 2 195 59 0.0 6 56 3 196 90 0.0 6 87 3 197 100 0.0 6 96 4 198 93 0.0 6 90 3 199 165 0.0 6 161 2 1 1 200 80 0.0 6 76 4 201 72 0.0 6 69 3 202 109 0.0 6 105 4 203 100 0.0 6 96 4 204 103 0.0 6 97 6 205 113 0.0 6 107 6 206 96 0.0 6 95 1 207 124 0.0 6 120 4 208 163 0.0 6 157 5 0 1 209 148 0.0 6 144 4 210 120 0.0 6 116 4 211 80 0.0 6 79 1 212 55 0.0 6 54 1 213 77 0.0 6 74 2 1 214 103 0.0 6 98 5 215 75 0.0 6 71 2 2 216 101 0.0 6 98 3 217 184 0.0 6 177 7 218 100 0.0 6 99 1 219 111 0.0 6 110 1 220 97 0.0 6 96 1 221 89 0.0 6 83 6 222 107 0.0 6 105 2 223 78 0.0 6 78 224 88 0.0 6 86 2 225 79 0.0 6 73 6 226 127 0.0 6 122 4 1 227 88 0.0 6 85 3 228 113 0.0 6 109 4 229 100 0.0 6 96 4 230 87 0.0 6 83 4 231 66 0.0 6 65 1 232 61 0.0 6 60 1 233 72 0.0 6 69 3 234 55 0.0 6 51 4 235 66 0.0 6 66 236 64 0.0 6 63 1 237 85 0.0 6 83 2 238 69 0.0 6 65 4 239 129 0.0 6 120 9 240 116 0.0 6 107 9 241 205 0.0 6 195 9 1 242 164 0.0 6 155 9 243 214 0.0 6 204 9 0 0 1 244 134 0.0 6 124 10 245 171 0.0 6 161 9 1 246 68 0.0 6 65 3 247 91 0.0 6 87 3 1 248 79 0.0 6 71 7 1 249 164 0.0 6 156 8 250 96 0.0 6 92 4 251 103 0.0 6 94 9 252 119 0.0 6 117 2 253 66 0.0 6 61 3 0 2 254 130 0.0 6 125 4 0 1 255 99 0.0 6 95 4 256 70 0.0 6 67 3 257 72 0.0 6 66 6 258 88 0.0 6 80 8 259 163 0.0 6 155 7 1 260 161 0.0 6 159 2 261 66 0.0 6 58 7 1 262 111 0.0 6 101 10 263 177 0.0 6 170 7 264 118 0.0 6 110 7 1 265 124 0.0 6 121 2 0 0 1 266 61 0.0 6 55 6 267 105 0.0 6 100 5 268 105 0.0 6 95 10 269 108 0.0 6 104 4 270 65 0.0 6 58 7 271 155 0.0 6 144 10 1 272 309 0.0 6 294 13 2 273 133 0.0 6 121 11 1 274 206 0.0 6 200 6 275 121 0.0 6 102 18 1 276 361 0.0 6 329 31 1 277 867 0.0 6 841 26 278 416 0.0 6 407 9 279 687 0.0 6 663 23 1 280 1146 0.0 6 988 148 8 2 281 34506 0.0 6 33964 534 6 1 0 0 1 282 14 0.0 6 10 4 283 5 0.0 6 5 284 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 45 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 1 0.0 6 1 71 2 0.0 6 2 72 1 0.0 6 1 73 1 0.0 6 1 75 3 0.0 6 3 81 3 0.0 6 3 89 2 0.0 6 2 90 3 0.0 6 3 91 1 0.0 6 1 92 1 0.0 6 1 93 1 0.0 6 1 97 1 0.0 6 1 98 3 0.0 6 3 99 1 0.0 6 1 101 1 0.0 6 1 102 1 0.0 6 1 117 2 0.0 6 2 123 1 0.0 6 1 125 3 0.0 6 3 134 1 0.0 6 1 145 1 0.0 6 1 147 2 0.0 6 2 150 2 0.0 6 2 151 1 0.0 6 1 153 1 0.0 6 1 174 1 0.0 6 1 182 1 0.0 6 1 197 1 0.0 6 1 203 1 0.0 6 1 225 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 49404 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 773.4 0 0 1 5 1 48.3 1 1 7 1 3.0 2 1 8 1 0.8 2 1 9 1 0.2 2 1 10 5 0.0 3 4 1 11 2 0.0 3 2 13 8 0.0 3 7 1 14 10 0.0 4 8 1 0 1 16 8 0.0 4 8 17 10 0.0 5 9 1 18 14 0.0 5 10 2 2 19 5 0.0 5 4 1 20 41 0.0 6 38 2 1 21 44 0.0 6 41 3 22 15 0.0 6 13 2 23 29 0.0 6 26 3 24 13 0.0 6 12 1 25 15 0.0 6 14 1 26 9 0.0 6 8 1 27 12 0.0 6 12 28 10 0.0 6 9 0 1 29 14 0.0 6 14 30 13 0.0 6 12 1 31 2 0.0 6 1 1 32 4 0.0 6 4 33 10 0.0 6 7 2 1 34 8 0.0 6 8 35 5 0.0 6 5 36 3 0.0 6 3 37 9 0.0 6 9 38 9 0.0 6 8 0 1 39 8 0.0 6 8 40 4 0.0 6 4 41 7 0.0 6 7 42 4 0.0 6 2 2 43 13 0.0 6 12 1 44 19 0.0 6 17 2 45 7 0.0 6 6 1 46 19 0.0 6 17 1 1 47 18 0.0 6 16 1 1 48 5 0.0 6 4 1 49 14 0.0 6 14 50 17 0.0 6 17 51 11 0.0 6 7 4 52 11 0.0 6 9 2 53 16 0.0 6 13 3 54 15 0.0 6 14 1 55 20 0.0 6 16 4 56 13 0.0 6 11 2 57 18 0.0 6 15 2 1 58 15 0.0 6 10 4 0 0 1 59 5 0.0 6 4 1 60 17 0.0 6 14 3 61 7 0.0 6 7 62 7 0.0 6 7 63 3 0.0 6 2 1 64 14 0.0 6 13 1 65 5 0.0 6 5 66 25 0.0 6 20 2 3 67 94 0.0 6 91 2 1 68 18 0.0 6 16 2 69 30 0.0 6 28 2 70 12 0.0 6 10 2 71 27 0.0 6 25 2 72 22 0.0 6 21 1 73 4 0.0 6 4 74 6 0.0 6 6 75 9 0.0 6 7 2 76 17 0.0 6 14 3 77 9 0.0 6 9 78 17 0.0 6 14 3 79 10 0.0 6 10 80 18 0.0 6 16 2 81 9 0.0 6 9 82 17 0.0 6 16 0 0 1 83 41 0.0 6 36 5 84 19 0.0 6 17 1 1 85 18 0.0 6 14 4 86 18 0.0 6 14 4 87 22 0.0 6 18 3 1 88 17 0.0 6 14 2 1 89 16 0.0 6 13 3 90 25 0.0 6 21 1 1 0 2 91 18 0.0 6 16 2 92 11 0.0 6 10 1 93 33 0.0 6 29 4 94 44 0.0 6 42 2 95 22 0.0 6 18 4 96 30 0.0 6 26 4 97 25 0.0 6 21 4 98 26 0.0 6 22 4 99 31 0.0 6 25 5 0 0 1 100 34 0.0 6 33 1 101 32 0.0 6 30 2 102 36 0.0 6 33 3 103 52 0.0 6 50 2 104 46 0.0 6 43 2 1 105 45 0.0 6 38 7 106 25 0.0 6 25 107 34 0.0 6 33 1 108 42 0.0 6 41 1 109 45 0.0 6 42 3 110 40 0.0 6 37 2 1 111 43 0.0 6 39 4 112 33 0.0 6 33 113 29 0.0 6 27 2 114 57 0.0 6 55 2 115 33 0.0 6 29 2 2 116 45 0.0 6 43 2 117 41 0.0 6 39 1 0 0 0 1 118 56 0.0 6 51 5 119 66 0.0 6 61 4 1 120 56 0.0 6 52 4 121 43 0.0 6 38 5 122 51 0.0 6 43 8 123 49 0.0 6 45 4 124 64 0.0 6 61 3 125 49 0.0 6 48 1 126 41 0.0 6 38 3 127 57 0.0 6 55 2 128 33 0.0 6 32 1 129 40 0.0 6 40 130 40 0.0 6 39 1 131 79 0.0 6 73 5 0 0 1 132 80 0.0 6 76 2 2 133 64 0.0 6 59 5 134 140 0.0 6 127 11 2 135 165 0.0 6 151 14 136 96 0.0 6 92 4 137 122 0.0 6 118 4 138 84 0.0 6 83 1 139 117 0.0 6 111 5 1 140 225 0.0 6 217 7 0 1 141 164 0.0 6 154 10 142 195 0.0 6 184 11 143 180 0.0 6 174 6 144 254 0.0 6 246 8 145 137 0.0 6 132 4 1 146 125 0.0 6 119 6 147 107 0.0 6 100 7 148 153 0.0 6 147 5 0 0 1 149 282 0.0 6 268 14 150 486 0.0 6 464 17 5 151 166 0.0 6 158 7 0 0 1 152 500 0.0 6 479 20 1 153 266 0.0 6 258 6 1 0 1 154 316 0.0 6 305 10 1 155 237 0.0 6 234 2 1 156 183 0.0 6 174 9 157 282 0.0 6 276 5 1 158 311 0.0 6 298 11 1 0 0 0 1 159 166 0.0 6 160 6 160 268 0.0 6 263 5 161 161 0.0 6 153 8 162 149 0.0 6 147 2 163 231 0.0 6 225 5 1 164 242 0.0 6 230 12 165 283 0.0 6 276 7 166 328 0.0 6 319 7 0 1 1 167 183 0.0 6 175 7 0 0 1 168 422 0.0 6 409 12 1 169 173 0.0 6 167 6 170 185 0.0 6 174 9 1 1 171 350 0.0 6 334 15 1 172 231 0.0 6 212 17 1 1 173 315 0.0 6 307 8 174 461 0.0 6 447 13 1 175 679 0.0 6 657 19 2 0 0 0 1 176 490 0.0 6 479 11 177 424 0.0 6 412 9 3 178 372 0.0 6 362 9 1 179 446 0.0 6 427 17 0 1 1 180 351 0.0 6 339 11 1 181 465 0.0 6 449 15 1 182 159 0.0 6 142 14 2 0 0 1 183 593 0.0 6 576 12 4 0 1 184 398 0.0 6 390 6 1 0 1 185 641 0.0 6 623 17 0 1 186 1101 0.0 6 1067 31 1 0 2 187 907 0.0 6 880 26 1 188 854 0.0 6 837 16 0 0 1 189 766 0.0 6 737 26 2 0 1 190 1194 0.0 6 1153 41 191 1300 0.0 6 1266 33 0 0 1 192 704 0.0 6 673 27 3 0 0 0 1 193 428 0.0 6 403 24 0 1 194 789 0.0 6 763 24 1 0 1 195 980 0.0 6 946 33 0 0 1 196 1811 0.0 6 1759 47 2 1 2 197 609 0.0 6 585 21 2 0 1 198 1228 0.0 6 1186 39 2 0 0 1 199 2235 0.0 6 2184 47 2 1 1 200 1055 0.0 6 1015 39 0 0 1 201 1323 0.0 6 1301 22 202 645 0.0 6 634 10 0 1 203 506 0.0 6 493 12 1 204 843 0.0 6 826 17 205 244 0.0 6 240 3 1 206 318 0.0 6 312 5 0 0 1 207 181 0.0 6 175 6 208 345 0.0 6 335 9 0 0 1 209 369 0.0 6 358 11 210 339 0.0 6 332 7 211 284 0.0 6 274 9 0 1 212 443 0.0 6 434 9 213 247 0.0 6 237 9 1 214 794 0.0 6 779 14 0 0 1 215 765 0.0 6 747 17 1 216 578 0.0 6 557 18 2 0 0 1 217 563 0.0 6 548 14 1 218 355 0.0 6 345 10 219 612 0.0 6 592 20 220 96 0.0 6 88 7 0 0 0 0 1 221 472 0.0 6 462 10 222 490 0.0 6 475 14 0 0 0 1 223 670 0.0 6 657 10 1 1 1 224 376 0.0 6 364 11 0 1 225 489 0.0 6 483 6 226 367 0.0 6 353 13 1 227 337 0.0 6 328 8 1 228 157 0.0 6 154 3 229 352 0.0 6 343 9 230 183 0.0 6 179 4 231 295 0.0 6 289 6 232 363 0.0 6 353 9 1 233 208 0.0 6 205 3 234 116 0.0 6 112 4 235 74 0.0 6 70 4 236 17 0.0 6 17 237 28 0.0 6 27 1 238 28 0.0 6 26 1 1 239 46 0.0 6 44 2 240 121 0.0 6 114 6 1 241 102 0.0 6 97 5 242 158 0.0 6 152 5 0 1 243 127 0.0 6 124 3 244 95 0.0 6 93 2 245 65 0.0 6 63 2 246 120 0.0 6 118 2 247 28 0.0 6 26 1 0 1 248 109 0.0 6 107 2 249 18 0.0 6 18 250 10 0.0 6 10 251 35 0.0 6 34 1 252 1 0.0 6 1 253 3 0.0 6 3 254 1 0.0 6 1 255 1 0.0 6 1 257 2 0.0 6 2 258 1 0.0 6 1 260 3 0.0 6 3 263 2 0.0 6 2 266 1 0.0 6 1 277 1 0.0 6 1 278 30 0.0 6 28 2 279 48 0.0 6 47 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 52 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 1 0.0 6 1 72 1 0.0 6 1 73 1 0.0 6 1 74 3 0.0 6 3 75 1 0.0 6 1 77 1 0.0 6 1 82 1 0.0 6 1 87 2 0.0 6 2 89 1 0.0 6 1 91 1 0.0 6 1 92 1 0.0 6 1 93 1 0.0 6 1 94 1 0.0 6 1 99 1 0.0 6 1 100 2 0.0 6 2 103 1 0.0 6 1 115 3 0.0 6 3 118 1 0.0 6 1 119 5 0.0 6 5 123 1 0.0 6 1 127 1 0.0 6 1 128 1 0.0 6 1 134 1 0.0 6 1 135 1 0.0 6 1 136 1 0.0 6 1 137 1 0.0 6 1 148 3 0.0 6 3 153 6 0.0 6 6 154 2 0.0 6 2 162 1 0.0 6 1 174 2 0.0 6 2 184 1 0.0 6 1 205 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_19_46_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_19_273_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_19_46_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_19_273_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.41 s (36 µs/read; 1.68 M reads/minute). === Summary === Total read pairs processed: 39,454 Read 1 with adapter: 39,452 (100.0%) Read 2 with adapter: 39,421 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 35 (0.1%) Pairs written (passing filters): 39,419 (99.9%) Total basepairs processed: 19,893,014 bp Read 1: 11,481,502 bp Read 2: 8,411,512 bp Total written (filtered): 2,365,256 bp (11.9%) Read 1: 1,182,416 bp Read 2: 1,182,840 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 39451 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 2 154.1 1 1 1 7 2 2.4 2 0 0 2 8 1 0.6 2 1 9 1 0.2 2 1 10 1 0.0 3 0 1 20 4 0.0 6 4 21 6 0.0 6 6 22 3 0.0 6 3 23 2 0.0 6 2 24 2 0.0 6 2 25 5 0.0 6 5 26 5 0.0 6 5 27 2 0.0 6 2 28 3 0.0 6 1 2 29 1 0.0 6 1 31 1 0.0 6 1 32 1 0.0 6 1 33 1 0.0 6 1 34 1 0.0 6 1 35 1 0.0 6 1 36 1 0.0 6 1 37 1 0.0 6 1 38 4 0.0 6 4 39 3 0.0 6 3 40 4 0.0 6 2 1 1 41 1 0.0 6 1 42 1 0.0 6 1 43 2 0.0 6 2 44 2 0.0 6 2 45 3 0.0 6 3 46 3 0.0 6 3 48 1 0.0 6 1 51 1 0.0 6 1 53 3 0.0 6 3 54 5 0.0 6 4 1 55 2 0.0 6 2 56 1 0.0 6 1 57 1 0.0 6 1 58 5 0.0 6 5 60 2 0.0 6 2 61 1 0.0 6 1 62 4 0.0 6 4 63 3 0.0 6 3 65 1 0.0 6 0 0 0 1 66 1 0.0 6 1 67 2 0.0 6 1 1 71 2 0.0 6 1 1 72 2 0.0 6 2 73 2 0.0 6 1 1 75 1 0.0 6 1 76 2 0.0 6 1 0 1 77 3 0.0 6 3 78 3 0.0 6 3 80 3 0.0 6 3 81 2 0.0 6 1 1 86 7 0.0 6 7 87 4 0.0 6 4 88 1 0.0 6 1 89 5 0.0 6 5 90 1 0.0 6 1 92 2 0.0 6 2 93 1 0.0 6 1 96 1 0.0 6 1 97 2 0.0 6 2 98 4 0.0 6 4 99 1 0.0 6 1 100 3 0.0 6 3 101 1 0.0 6 1 102 6 0.0 6 6 103 3 0.0 6 3 104 1 0.0 6 1 105 2 0.0 6 2 106 2 0.0 6 2 107 1 0.0 6 1 108 6 0.0 6 6 110 1 0.0 6 1 111 1 0.0 6 1 112 4 0.0 6 3 1 113 5 0.0 6 5 114 2 0.0 6 2 115 1 0.0 6 1 117 5 0.0 6 5 118 1 0.0 6 1 119 6 0.0 6 6 121 5 0.0 6 3 2 122 7 0.0 6 7 123 10 0.0 6 9 1 124 6 0.0 6 6 125 5 0.0 6 5 126 5 0.0 6 4 0 1 127 3 0.0 6 3 128 8 0.0 6 7 1 129 4 0.0 6 4 130 3 0.0 6 3 131 2 0.0 6 1 1 132 4 0.0 6 4 133 7 0.0 6 7 134 5 0.0 6 5 135 8 0.0 6 8 136 7 0.0 6 6 1 137 16 0.0 6 15 1 138 22 0.0 6 21 1 139 4 0.0 6 3 1 140 12 0.0 6 11 1 141 7 0.0 6 7 142 20 0.0 6 19 1 143 25 0.0 6 23 2 144 34 0.0 6 34 145 7 0.0 6 7 146 5 0.0 6 5 147 26 0.0 6 26 148 15 0.0 6 14 1 149 14 0.0 6 14 150 18 0.0 6 18 151 14 0.0 6 14 152 11 0.0 6 10 1 153 6 0.0 6 5 1 154 8 0.0 6 8 155 7 0.0 6 6 1 156 9 0.0 6 9 157 20 0.0 6 19 1 158 7 0.0 6 7 159 17 0.0 6 16 1 160 9 0.0 6 9 161 16 0.0 6 15 1 162 10 0.0 6 10 163 24 0.0 6 24 164 10 0.0 6 10 165 20 0.0 6 20 166 8 0.0 6 8 167 15 0.0 6 14 1 168 6 0.0 6 5 1 169 5 0.0 6 5 170 21 0.0 6 19 2 171 18 0.0 6 17 1 172 16 0.0 6 16 173 22 0.0 6 22 174 19 0.0 6 19 175 28 0.0 6 27 1 176 24 0.0 6 23 1 177 26 0.0 6 25 1 178 14 0.0 6 13 1 179 36 0.0 6 35 1 180 28 0.0 6 28 181 34 0.0 6 34 182 34 0.0 6 34 183 27 0.0 6 26 1 184 52 0.0 6 51 1 185 45 0.0 6 44 1 186 70 0.0 6 69 1 187 42 0.0 6 42 188 30 0.0 6 30 189 36 0.0 6 33 3 190 52 0.0 6 52 191 64 0.0 6 57 7 192 40 0.0 6 38 2 193 65 0.0 6 64 1 194 70 0.0 6 67 3 195 54 0.0 6 52 2 196 60 0.0 6 60 197 64 0.0 6 64 198 78 0.0 6 76 2 199 92 0.0 6 89 3 200 91 0.0 6 88 3 201 91 0.0 6 88 3 202 79 0.0 6 77 2 203 131 0.0 6 124 6 1 204 101 0.0 6 99 2 205 139 0.0 6 135 4 206 92 0.0 6 87 5 207 132 0.0 6 126 6 208 172 0.0 6 164 8 209 118 0.0 6 117 1 210 111 0.0 6 111 211 95 0.0 6 90 5 212 34 0.0 6 34 213 130 0.0 6 125 4 1 214 108 0.0 6 107 1 215 68 0.0 6 66 1 1 216 78 0.0 6 74 4 217 220 0.0 6 214 6 218 124 0.0 6 115 8 1 219 122 0.0 6 119 3 220 114 0.0 6 110 3 1 221 115 0.0 6 110 5 222 121 0.0 6 120 1 223 141 0.0 6 136 5 224 100 0.0 6 97 3 225 150 0.0 6 146 4 226 241 0.0 6 235 6 227 248 0.0 6 238 10 228 274 0.0 6 270 3 1 229 192 0.0 6 186 6 230 199 0.0 6 194 5 231 125 0.0 6 122 3 232 194 0.0 6 188 6 233 203 0.0 6 194 9 234 228 0.0 6 225 3 235 177 0.0 6 171 6 236 135 0.0 6 130 5 237 138 0.0 6 133 5 238 141 0.0 6 136 3 1 0 0 0 1 239 137 0.0 6 132 5 240 166 0.0 6 159 7 241 245 0.0 6 243 2 242 241 0.0 6 232 9 243 213 0.0 6 207 6 244 195 0.0 6 189 5 1 245 249 0.0 6 243 6 246 98 0.0 6 85 12 1 247 140 0.0 6 134 6 248 114 0.0 6 107 7 249 286 0.0 6 279 7 250 168 0.0 6 161 7 251 215 0.0 6 208 7 252 246 0.0 6 243 3 253 194 0.0 6 189 5 254 377 0.0 6 367 10 255 169 0.0 6 166 3 256 288 0.0 6 278 10 257 168 0.0 6 160 8 258 250 0.0 6 235 14 1 259 336 0.0 6 326 10 260 546 0.0 6 534 12 261 173 0.0 6 168 5 262 266 0.0 6 255 11 263 412 0.0 6 401 10 1 264 340 0.0 6 336 4 265 342 0.0 6 332 10 266 280 0.0 6 274 5 1 267 329 0.0 6 321 8 268 361 0.0 6 344 17 269 273 0.0 6 265 8 270 190 0.0 6 181 8 1 271 429 0.0 6 402 27 272 567 0.0 6 557 10 273 412 0.0 6 397 15 274 445 0.0 6 431 13 1 275 596 0.0 6 578 17 1 276 946 0.0 6 919 27 277 823 0.0 6 805 18 278 504 0.0 6 495 9 279 856 0.0 6 836 18 0 2 280 1343 0.0 6 1263 75 5 281 17037 0.0 6 16787 250 282 10 0.0 6 8 0 1 1 283 1 0.0 6 1 284 2 0.0 6 2 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 39413 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 10 1 0.0 3 1 11 2 0.0 3 2 12 2 0.0 3 2 13 9 0.0 3 9 14 2 0.0 4 1 1 16 2 0.0 4 2 17 4 0.0 5 4 18 4 0.0 5 3 1 19 5 0.0 5 3 1 0 1 20 29 0.0 6 29 21 21 0.0 6 21 22 16 0.0 6 15 1 23 16 0.0 6 16 24 10 0.0 6 10 25 4 0.0 6 4 26 6 0.0 6 6 27 9 0.0 6 9 28 5 0.0 6 5 29 1 0.0 6 1 30 4 0.0 6 4 31 3 0.0 6 3 32 3 0.0 6 3 33 2 0.0 6 2 34 2 0.0 6 2 35 5 0.0 6 5 36 3 0.0 6 3 37 6 0.0 6 6 38 12 0.0 6 12 39 9 0.0 6 7 2 40 9 0.0 6 9 41 4 0.0 6 4 42 4 0.0 6 4 43 3 0.0 6 2 1 44 7 0.0 6 6 1 45 5 0.0 6 5 46 18 0.0 6 16 2 47 9 0.0 6 8 0 1 48 5 0.0 6 5 49 10 0.0 6 9 1 50 10 0.0 6 10 51 6 0.0 6 6 52 7 0.0 6 7 53 7 0.0 6 6 1 54 17 0.0 6 15 2 55 5 0.0 6 5 56 11 0.0 6 10 1 57 8 0.0 6 6 2 58 5 0.0 6 4 1 59 9 0.0 6 8 1 60 13 0.0 6 13 61 8 0.0 6 7 1 62 7 0.0 6 7 63 4 0.0 6 4 64 12 0.0 6 8 4 65 5 0.0 6 4 1 66 15 0.0 6 15 67 11 0.0 6 8 2 1 68 18 0.0 6 18 69 80 0.0 6 76 4 70 7 0.0 6 7 71 33 0.0 6 32 1 72 18 0.0 6 17 1 73 15 0.0 6 15 74 19 0.0 6 19 75 6 0.0 6 5 1 76 14 0.0 6 13 1 77 14 0.0 6 13 1 78 18 0.0 6 15 3 79 14 0.0 6 13 0 0 0 1 80 12 0.0 6 10 2 81 11 0.0 6 10 1 82 22 0.0 6 18 4 83 16 0.0 6 14 1 0 0 1 84 19 0.0 6 18 1 85 23 0.0 6 23 86 21 0.0 6 18 3 87 20 0.0 6 19 1 88 6 0.0 6 6 89 21 0.0 6 18 3 90 18 0.0 6 17 1 91 19 0.0 6 18 1 92 11 0.0 6 9 1 0 0 1 93 35 0.0 6 29 4 2 94 44 0.0 6 43 1 95 16 0.0 6 15 1 96 21 0.0 6 18 3 97 28 0.0 6 28 98 22 0.0 6 20 2 99 21 0.0 6 19 2 100 17 0.0 6 16 1 101 14 0.0 6 13 1 102 46 0.0 6 41 4 1 103 55 0.0 6 51 3 1 104 46 0.0 6 43 2 1 105 30 0.0 6 28 2 106 35 0.0 6 35 107 39 0.0 6 35 4 108 35 0.0 6 31 3 1 109 18 0.0 6 16 2 110 19 0.0 6 19 111 25 0.0 6 24 1 112 25 0.0 6 25 113 16 0.0 6 14 1 0 1 114 29 0.0 6 25 4 115 33 0.0 6 30 2 0 0 1 116 41 0.0 6 37 4 117 27 0.0 6 27 118 34 0.0 6 33 1 119 43 0.0 6 39 3 1 120 72 0.0 6 70 1 0 0 1 121 35 0.0 6 33 2 122 40 0.0 6 40 123 55 0.0 6 55 124 78 0.0 6 75 2 1 125 34 0.0 6 32 1 1 126 42 0.0 6 39 3 127 44 0.0 6 40 4 128 37 0.0 6 34 3 129 40 0.0 6 37 3 130 39 0.0 6 38 1 131 88 0.0 6 84 4 132 113 0.0 6 106 7 133 111 0.0 6 102 9 134 121 0.0 6 111 9 1 135 191 0.0 6 186 3 2 136 94 0.0 6 90 3 1 137 142 0.0 6 138 3 1 138 98 0.0 6 93 4 0 1 139 103 0.0 6 101 1 1 140 139 0.0 6 138 1 141 146 0.0 6 142 3 1 142 214 0.0 6 209 4 0 0 1 143 149 0.0 6 140 9 144 270 0.0 6 261 9 145 115 0.0 6 110 5 146 99 0.0 6 92 6 0 0 1 147 65 0.0 6 65 148 116 0.0 6 114 2 149 192 0.0 6 187 5 150 297 0.0 6 286 8 3 151 132 0.0 6 122 10 152 402 0.0 6 389 11 2 153 201 0.0 6 188 13 154 249 0.0 6 239 10 155 286 0.0 6 274 11 0 0 0 1 156 170 0.0 6 162 7 1 157 306 0.0 6 297 7 0 0 2 158 320 0.0 6 313 7 159 149 0.0 6 144 5 160 310 0.0 6 300 10 161 224 0.0 6 215 8 0 1 162 203 0.0 6 200 3 163 257 0.0 6 248 8 0 0 1 164 230 0.0 6 221 9 165 280 0.0 6 275 5 166 410 0.0 6 397 12 1 167 140 0.0 6 133 7 168 519 0.0 6 505 14 169 96 0.0 6 92 4 170 242 0.0 6 236 5 0 0 1 171 477 0.0 6 463 13 0 0 1 172 108 0.0 6 105 2 1 173 169 0.0 6 160 8 0 1 174 274 0.0 6 269 5 175 581 0.0 6 560 19 2 176 322 0.0 6 311 10 1 177 527 0.0 6 515 10 2 178 376 0.0 6 363 11 1 0 1 179 471 0.0 6 460 10 0 1 180 451 0.0 6 434 15 0 0 1 1 181 489 0.0 6 481 8 182 154 0.0 6 146 7 0 0 1 183 413 0.0 6 403 8 1 0 0 1 184 130 0.0 6 127 2 1 185 265 0.0 6 251 12 1 0 1 186 1012 0.0 6 997 14 1 187 870 0.0 6 834 34 2 188 938 0.0 6 918 19 0 0 1 189 746 0.0 6 724 21 0 0 1 190 1157 0.0 6 1126 30 0 0 0 1 191 916 0.0 6 883 31 1 0 1 192 726 0.0 6 708 18 193 561 0.0 6 547 14 194 658 0.0 6 640 18 195 1027 0.0 6 998 27 0 0 1 1 196 1851 0.0 6 1802 44 1 2 2 197 318 0.0 6 309 9 198 1363 0.0 6 1327 32 4 199 2307 0.0 6 2262 43 1 0 1 200 440 0.0 6 430 9 1 201 618 0.0 6 598 17 0 1 1 1 202 353 0.0 6 342 11 203 366 0.0 6 354 12 204 484 0.0 6 473 11 205 236 0.0 6 228 8 206 163 0.0 6 159 4 207 127 0.0 6 122 5 208 287 0.0 6 277 10 209 222 0.0 6 219 1 1 0 1 210 153 0.0 6 148 5 211 260 0.0 6 257 3 212 288 0.0 6 280 8 213 96 0.0 6 94 2 214 138 0.0 6 136 2 215 164 0.0 6 158 6 216 480 0.0 6 468 12 217 311 0.0 6 299 11 0 1 218 240 0.0 6 230 9 0 1 219 216 0.0 6 209 7 220 156 0.0 6 151 5 221 203 0.0 6 195 8 222 302 0.0 6 290 12 223 772 0.0 6 757 15 224 179 0.0 6 175 4 225 222 0.0 6 216 5 0 0 1 226 145 0.0 6 142 3 227 213 0.0 6 209 4 228 76 0.0 6 74 2 229 166 0.0 6 161 5 230 91 0.0 6 88 3 231 132 0.0 6 129 3 232 124 0.0 6 123 1 233 188 0.0 6 179 8 1 234 123 0.0 6 120 3 235 42 0.0 6 42 236 32 0.0 6 29 2 0 1 237 22 0.0 6 22 238 12 0.0 6 11 1 239 8 0.0 6 8 240 54 0.0 6 53 1 241 26 0.0 6 22 4 242 67 0.0 6 66 1 243 101 0.0 6 97 4 244 60 0.0 6 57 3 245 24 0.0 6 23 1 246 24 0.0 6 24 247 14 0.0 6 14 248 19 0.0 6 19 249 24 0.0 6 24 250 16 0.0 6 15 1 251 13 0.0 6 13 252 13 0.0 6 13 253 13 0.0 6 13 254 18 0.0 6 17 1 255 12 0.0 6 12 256 4 0.0 6 3 1 257 1 0.0 6 1 279 2 0.0 6 2 280 36 0.0 6 35 1 281 139 0.0 6 130 9 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 8 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 73 1 0.0 6 1 85 1 0.0 6 1 86 3 0.0 6 3 118 1 0.0 6 1 132 1 0.0 6 1 133 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_20_47_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_20_274_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_20_47_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_20_274_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.41 s (21 µs/read; 2.79 M reads/minute). === Summary === Total read pairs processed: 65,680 Read 1 with adapter: 65,674 (100.0%) Read 2 with adapter: 65,633 (99.9%) == Read fate breakdown == Pairs that were too short: 35 (0.1%) Pairs discarded as untrimmed: 53 (0.1%) Pairs written (passing filters): 65,592 (99.9%) Total basepairs processed: 27,708,620 bp Read 1: 15,319,972 bp Read 2: 12,388,648 bp Total written (filtered): 4,188,072 bp (15.1%) Read 1: 2,048,576 bp Read 2: 2,139,496 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 64183 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1026.2 0 1 4 1 256.6 1 0 1 6 1 16.0 1 0 0 1 8 2 1.0 2 0 2 9 2 0.3 2 1 1 10 2 0.1 3 2 15 4 0.0 4 3 1 16 4 0.0 4 1 3 17 2 0.0 5 1 1 19 1 0.0 5 0 1 20 141 0.0 6 136 4 1 21 80 0.0 6 79 1 22 58 0.0 6 57 1 23 44 0.0 6 42 2 24 21 0.0 6 21 25 25 0.0 6 24 0 1 26 20 0.0 6 20 27 10 0.0 6 10 28 9 0.0 6 9 29 14 0.0 6 13 1 30 9 0.0 6 9 31 9 0.0 6 9 32 10 0.0 6 10 33 5 0.0 6 5 34 8 0.0 6 7 1 35 7 0.0 6 7 36 4 0.0 6 4 37 6 0.0 6 6 38 4 0.0 6 4 39 9 0.0 6 9 40 7 0.0 6 7 41 7 0.0 6 7 42 3 0.0 6 3 43 6 0.0 6 6 44 6 0.0 6 6 45 8 0.0 6 7 1 46 5 0.0 6 5 47 6 0.0 6 6 48 6 0.0 6 6 49 5 0.0 6 4 1 50 7 0.0 6 6 1 51 8 0.0 6 8 52 9 0.0 6 8 0 1 53 9 0.0 6 6 2 1 54 2 0.0 6 2 55 9 0.0 6 9 56 4 0.0 6 4 57 3 0.0 6 3 58 6 0.0 6 6 59 5 0.0 6 5 60 2 0.0 6 2 61 3 0.0 6 3 62 3 0.0 6 3 63 6 0.0 6 6 64 3 0.0 6 3 65 8 0.0 6 8 66 5 0.0 6 4 1 67 12 0.0 6 10 1 0 0 0 0 1 68 5 0.0 6 4 1 69 7 0.0 6 7 70 7 0.0 6 7 71 6 0.0 6 6 72 8 0.0 6 8 73 6 0.0 6 6 74 12 0.0 6 11 1 75 8 0.0 6 8 76 8 0.0 6 8 77 5 0.0 6 5 78 4 0.0 6 4 79 6 0.0 6 6 80 5 0.0 6 5 81 10 0.0 6 8 2 82 6 0.0 6 6 83 15 0.0 6 15 84 8 0.0 6 7 1 85 28 0.0 6 27 1 86 14 0.0 6 13 1 87 14 0.0 6 12 2 88 10 0.0 6 10 89 10 0.0 6 9 1 90 9 0.0 6 9 91 9 0.0 6 8 0 0 0 0 0 1 92 7 0.0 6 7 93 9 0.0 6 8 1 94 11 0.0 6 10 0 1 95 7 0.0 6 7 96 6 0.0 6 6 97 8 0.0 6 8 98 6 0.0 6 6 99 9 0.0 6 9 100 9 0.0 6 9 101 13 0.0 6 11 1 0 0 0 0 1 102 20 0.0 6 20 103 8 0.0 6 8 104 7 0.0 6 7 105 13 0.0 6 13 106 23 0.0 6 23 107 16 0.0 6 15 1 108 16 0.0 6 15 0 0 0 0 0 1 109 14 0.0 6 13 1 110 19 0.0 6 19 111 12 0.0 6 12 112 17 0.0 6 16 0 0 0 1 113 12 0.0 6 11 1 114 22 0.0 6 22 115 17 0.0 6 16 1 116 26 0.0 6 25 0 0 0 1 117 21 0.0 6 21 118 23 0.0 6 23 119 19 0.0 6 19 120 16 0.0 6 16 121 20 0.0 6 20 122 25 0.0 6 24 0 0 0 0 1 123 16 0.0 6 16 124 23 0.0 6 22 1 125 24 0.0 6 24 126 22 0.0 6 22 127 28 0.0 6 27 1 128 26 0.0 6 25 1 129 29 0.0 6 29 130 24 0.0 6 24 131 34 0.0 6 34 132 34 0.0 6 33 0 1 133 36 0.0 6 35 0 0 1 134 29 0.0 6 29 135 39 0.0 6 39 136 40 0.0 6 40 137 35 0.0 6 35 138 43 0.0 6 42 1 139 41 0.0 6 41 140 38 0.0 6 38 141 47 0.0 6 47 142 71 0.0 6 70 1 143 182 0.0 6 180 2 144 441 0.0 6 440 1 145 540 0.0 6 540 146 394 0.0 6 394 147 334 0.0 6 332 2 148 574 0.0 6 574 149 470 0.0 6 470 150 532 0.0 6 532 151 548 0.0 6 546 2 152 491 0.0 6 491 153 516 0.0 6 515 1 154 432 0.0 6 432 155 355 0.0 6 354 1 156 349 0.0 6 347 1 0 0 0 0 1 157 339 0.0 6 337 2 158 302 0.0 6 302 159 317 0.0 6 317 160 379 0.0 6 379 161 405 0.0 6 404 0 1 162 432 0.0 6 431 0 0 0 0 0 1 163 412 0.0 6 411 1 164 395 0.0 6 394 0 0 0 0 0 1 165 365 0.0 6 364 1 166 389 0.0 6 389 167 375 0.0 6 374 1 168 309 0.0 6 307 2 169 364 0.0 6 363 1 170 396 0.0 6 396 171 357 0.0 6 354 3 172 391 0.0 6 388 3 173 364 0.0 6 364 174 367 0.0 6 363 4 175 431 0.0 6 428 1 0 0 0 1 1 176 325 0.0 6 323 2 177 350 0.0 6 348 2 178 380 0.0 6 380 179 317 0.0 6 317 180 430 0.0 6 427 3 181 1079 0.0 6 1076 2 0 1 182 481 0.0 6 480 0 0 1 183 341 0.0 6 340 1 184 417 0.0 6 415 2 185 411 0.0 6 411 186 417 0.0 6 416 1 187 383 0.0 6 381 2 188 452 0.0 6 451 1 189 351 0.0 6 350 1 190 401 0.0 6 398 3 191 349 0.0 6 347 2 192 411 0.0 6 409 2 193 468 0.0 6 468 194 519 0.0 6 519 195 467 0.0 6 467 196 601 0.0 6 598 3 197 639 0.0 6 638 1 198 649 0.0 6 646 3 199 788 0.0 6 787 0 1 200 817 0.0 6 814 2 1 201 641 0.0 6 641 202 606 0.0 6 603 2 0 0 0 1 203 706 0.0 6 705 1 204 579 0.0 6 576 3 205 893 0.0 6 889 4 206 660 0.0 6 656 4 207 713 0.0 6 713 208 924 0.0 6 921 3 209 667 0.0 6 665 2 210 804 0.0 6 802 1 0 0 0 1 211 662 0.0 6 658 3 0 0 0 0 1 212 652 0.0 6 651 0 1 213 746 0.0 6 744 1 0 0 0 1 214 734 0.0 6 732 2 215 705 0.0 6 701 2 0 0 1 1 216 838 0.0 6 829 6 1 0 0 2 217 966 0.0 6 960 5 0 0 1 218 749 0.0 6 747 2 219 758 0.0 6 752 6 220 813 0.0 6 810 1 0 0 0 0 2 221 716 0.0 6 713 3 222 572 0.0 6 566 5 1 223 647 0.0 6 644 3 224 553 0.0 6 551 2 225 575 0.0 6 572 3 226 681 0.0 6 678 2 0 0 1 227 763 0.0 6 762 1 228 717 0.0 6 716 1 229 612 0.0 6 610 2 230 592 0.0 6 591 1 231 539 0.0 6 537 1 1 232 581 0.0 6 579 2 233 604 0.0 6 600 4 234 563 0.0 6 560 3 235 553 0.0 6 552 1 236 533 0.0 6 533 237 482 0.0 6 479 3 238 488 0.0 6 485 3 239 503 0.0 6 500 3 240 437 0.0 6 435 2 241 465 0.0 6 465 242 405 0.0 6 403 2 243 421 0.0 6 420 1 244 411 0.0 6 407 4 245 381 0.0 6 380 1 246 333 0.0 6 332 1 247 310 0.0 6 310 248 261 0.0 6 260 1 249 242 0.0 6 242 250 259 0.0 6 258 1 251 244 0.0 6 242 1 1 252 247 0.0 6 244 3 253 220 0.0 6 219 0 0 0 0 0 1 254 215 0.0 6 215 255 211 0.0 6 210 1 256 223 0.0 6 221 2 257 158 0.0 6 158 258 170 0.0 6 170 259 194 0.0 6 193 0 0 0 1 260 186 0.0 6 185 0 1 261 208 0.0 6 208 262 178 0.0 6 178 263 144 0.0 6 143 1 264 132 0.0 6 131 1 265 147 0.0 6 147 266 133 0.0 6 132 0 0 0 0 1 267 130 0.0 6 129 1 268 130 0.0 6 130 269 110 0.0 6 109 1 270 102 0.0 6 102 271 136 0.0 6 136 272 90 0.0 6 90 273 92 0.0 6 92 274 87 0.0 6 86 1 275 106 0.0 6 104 2 276 124 0.0 6 124 277 140 0.0 6 138 1 0 0 0 0 1 278 171 0.0 6 171 279 252 0.0 6 252 280 771 0.0 6 767 3 0 0 0 0 1 281 1195 0.0 6 1190 4 0 0 0 1 282 4 0.0 6 3 1 283 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1491 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 59 3 0.0 6 0 0 2 1 60 5 0.0 6 0 5 61 42 0.0 6 41 1 65 1 0.0 6 1 68 1 0.0 6 1 69 1 0.0 6 1 70 3 0.0 6 3 71 12 0.0 6 12 72 10 0.0 6 10 73 9 0.0 6 9 74 14 0.0 6 14 75 10 0.0 6 10 76 14 0.0 6 14 77 5 0.0 6 5 78 15 0.0 6 15 79 14 0.0 6 14 80 8 0.0 6 8 81 12 0.0 6 12 82 13 0.0 6 13 83 10 0.0 6 10 84 13 0.0 6 13 85 6 0.0 6 6 86 9 0.0 6 9 87 5 0.0 6 5 88 12 0.0 6 12 89 12 0.0 6 12 90 9 0.0 6 9 91 3 0.0 6 3 92 7 0.0 6 7 93 13 0.0 6 13 94 9 0.0 6 9 95 6 0.0 6 6 96 12 0.0 6 12 97 12 0.0 6 12 98 9 0.0 6 9 99 8 0.0 6 8 100 6 0.0 6 6 101 9 0.0 6 9 102 14 0.0 6 14 103 6 0.0 6 6 104 4 0.0 6 4 105 9 0.0 6 9 106 11 0.0 6 11 107 16 0.0 6 16 108 27 0.0 6 27 109 8 0.0 6 8 110 9 0.0 6 9 111 7 0.0 6 7 112 9 0.0 6 9 113 6 0.0 6 6 114 11 0.0 6 11 115 3 0.0 6 3 116 2 0.0 6 2 117 8 0.0 6 8 118 5 0.0 6 5 119 3 0.0 6 3 120 6 0.0 6 6 121 4 0.0 6 4 122 8 0.0 6 8 123 4 0.0 6 4 124 7 0.0 6 7 125 5 0.0 6 5 126 9 0.0 6 9 127 13 0.0 6 13 128 11 0.0 6 11 129 10 0.0 6 10 130 10 0.0 6 10 131 9 0.0 6 9 132 15 0.0 6 15 133 4 0.0 6 4 134 16 0.0 6 16 135 9 0.0 6 9 136 9 0.0 6 9 137 9 0.0 6 9 138 9 0.0 6 9 139 10 0.0 6 10 140 14 0.0 6 14 141 6 0.0 6 6 142 12 0.0 6 12 143 11 0.0 6 11 144 10 0.0 6 10 145 9 0.0 6 9 146 9 0.0 6 9 147 9 0.0 6 9 148 8 0.0 6 7 1 149 5 0.0 6 5 150 12 0.0 6 12 151 10 0.0 6 10 152 11 0.0 6 11 153 9 0.0 6 9 154 5 0.0 6 5 155 15 0.0 6 15 156 11 0.0 6 11 157 11 0.0 6 11 158 16 0.0 6 16 159 7 0.0 6 7 160 13 0.0 6 13 161 7 0.0 6 7 162 8 0.0 6 8 163 8 0.0 6 8 164 13 0.0 6 13 165 19 0.0 6 19 166 7 0.0 6 7 167 9 0.0 6 9 168 14 0.0 6 14 169 9 0.0 6 9 170 5 0.0 6 5 171 15 0.0 6 15 172 11 0.0 6 11 173 7 0.0 6 7 174 8 0.0 6 8 175 10 0.0 6 10 176 10 0.0 6 10 177 7 0.0 6 7 178 7 0.0 6 7 179 10 0.0 6 10 180 10 0.0 6 10 181 8 0.0 6 8 182 4 0.0 6 4 183 7 0.0 6 7 184 4 0.0 6 4 185 9 0.0 6 9 186 9 0.0 6 9 187 6 0.0 6 6 188 7 0.0 6 7 189 8 0.0 6 8 190 6 0.0 6 6 191 10 0.0 6 10 192 6 0.0 6 6 193 6 0.0 6 6 194 7 0.0 6 7 195 9 0.0 6 9 196 6 0.0 6 6 197 6 0.0 6 6 198 5 0.0 6 5 199 5 0.0 6 5 200 10 0.0 6 10 201 12 0.0 6 12 202 5 0.0 6 5 203 8 0.0 6 8 204 13 0.0 6 13 205 6 0.0 6 6 206 5 0.0 6 5 207 9 0.0 6 9 208 4 0.0 6 4 209 4 0.0 6 4 210 2 0.0 6 2 211 5 0.0 6 5 212 5 0.0 6 5 213 8 0.0 6 8 214 6 0.0 6 6 215 5 0.0 6 5 216 5 0.0 6 5 217 4 0.0 6 4 218 7 0.0 6 7 219 4 0.0 6 4 220 4 0.0 6 4 221 4 0.0 6 4 222 5 0.0 6 5 223 3 0.0 6 3 224 6 0.0 6 6 225 3 0.0 6 3 226 8 0.0 6 7 0 0 0 0 0 1 227 4 0.0 6 4 228 5 0.0 6 5 229 1 0.0 6 1 230 3 0.0 6 3 231 5 0.0 6 5 232 1 0.0 6 1 233 3 0.0 6 3 234 2 0.0 6 2 235 2 0.0 6 2 236 2 0.0 6 2 237 5 0.0 6 5 238 2 0.0 6 2 239 3 0.0 6 3 240 2 0.0 6 2 242 3 0.0 6 3 243 3 0.0 6 3 244 1 0.0 6 1 245 1 0.0 6 1 246 3 0.0 6 3 247 2 0.0 6 2 248 3 0.0 6 3 250 2 0.0 6 2 251 2 0.0 6 2 252 1 0.0 6 1 253 1 0.0 6 1 254 3 0.0 6 3 255 1 0.0 6 1 256 2 0.0 6 2 257 3 0.0 6 3 258 1 0.0 6 1 259 2 0.0 6 2 260 2 0.0 6 2 261 2 0.0 6 2 262 1 0.0 6 1 263 1 0.0 6 1 266 2 0.0 6 2 267 1 0.0 6 1 268 1 0.0 6 1 271 1 0.0 6 1 277 2 0.0 6 2 280 2 0.0 6 2 282 1 0.0 6 1 283 1 0.0 6 1 284 1 0.0 6 1 290 1 0.0 6 1 291 2 0.0 6 2 294 1 0.0 6 1 297 1 0.0 6 1 302 1 0.0 6 1 308 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 64327 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 1026.2 0 0 4 9 1 0.3 2 1 10 4 0.1 3 3 0 1 11 3 0.0 3 3 12 1 0.0 3 1 13 5 0.0 3 5 14 12 0.0 4 11 1 15 1 0.0 4 0 0 1 16 11 0.0 4 8 3 17 23 0.0 5 21 2 18 19 0.0 5 19 19 13 0.0 5 13 20 114 0.0 6 111 2 1 21 52 0.0 6 52 22 26 0.0 6 26 23 28 0.0 6 27 1 24 21 0.0 6 17 3 1 25 18 0.0 6 18 26 23 0.0 6 22 1 27 15 0.0 6 15 28 13 0.0 6 13 29 19 0.0 6 17 2 30 11 0.0 6 11 31 11 0.0 6 10 1 32 14 0.0 6 13 0 0 0 1 33 14 0.0 6 13 1 34 20 0.0 6 20 35 16 0.0 6 14 2 36 14 0.0 6 14 37 11 0.0 6 10 1 38 11 0.0 6 9 1 0 0 0 0 1 39 16 0.0 6 11 5 40 6 0.0 6 6 41 12 0.0 6 12 42 8 0.0 6 5 0 2 0 0 1 43 19 0.0 6 17 2 44 8 0.0 6 6 1 1 45 17 0.0 6 14 3 46 16 0.0 6 14 1 0 0 0 1 47 20 0.0 6 20 48 17 0.0 6 16 1 49 8 0.0 6 8 50 14 0.0 6 14 51 15 0.0 6 15 52 13 0.0 6 12 0 0 1 53 25 0.0 6 22 1 1 1 54 23 0.0 6 20 3 55 16 0.0 6 15 1 56 21 0.0 6 20 1 57 20 0.0 6 20 58 20 0.0 6 18 2 59 19 0.0 6 17 2 60 15 0.0 6 14 1 61 13 0.0 6 11 2 62 24 0.0 6 21 1 1 0 0 1 63 23 0.0 6 22 1 64 32 0.0 6 29 3 65 33 0.0 6 30 3 66 46 0.0 6 43 0 2 1 67 118 0.0 6 113 4 0 1 68 52 0.0 6 48 4 69 62 0.0 6 60 2 70 31 0.0 6 30 1 71 50 0.0 6 49 1 72 30 0.0 6 29 1 73 20 0.0 6 19 1 74 37 0.0 6 33 3 0 0 0 1 75 27 0.0 6 25 2 76 26 0.0 6 26 77 28 0.0 6 27 1 78 31 0.0 6 28 2 0 0 1 79 40 0.0 6 38 2 80 38 0.0 6 36 2 81 53 0.0 6 53 82 51 0.0 6 48 3 83 56 0.0 6 55 0 0 1 84 39 0.0 6 36 3 85 45 0.0 6 42 3 86 35 0.0 6 33 2 87 38 0.0 6 38 88 57 0.0 6 57 89 39 0.0 6 37 1 1 90 59 0.0 6 57 1 0 1 91 66 0.0 6 63 2 1 92 44 0.0 6 42 1 1 93 65 0.0 6 64 1 94 75 0.0 6 71 4 95 52 0.0 6 50 2 96 62 0.0 6 61 1 97 58 0.0 6 55 3 98 73 0.0 6 70 3 99 58 0.0 6 56 2 100 66 0.0 6 65 1 101 83 0.0 6 79 3 0 1 102 58 0.0 6 56 2 103 82 0.0 6 77 4 0 0 0 1 104 167 0.0 6 160 6 0 1 105 82 0.0 6 76 3 0 1 1 1 106 66 0.0 6 65 1 107 87 0.0 6 85 2 108 89 0.0 6 89 109 96 0.0 6 93 2 0 0 1 110 122 0.0 6 118 3 0 1 111 108 0.0 6 106 2 112 129 0.0 6 123 5 1 113 108 0.0 6 104 3 1 114 113 0.0 6 113 115 159 0.0 6 153 5 0 0 0 0 1 116 111 0.0 6 111 117 157 0.0 6 154 2 0 0 0 1 118 141 0.0 6 137 4 119 144 0.0 6 140 3 0 0 0 0 1 120 138 0.0 6 136 2 121 174 0.0 6 171 2 1 122 163 0.0 6 159 3 0 0 0 1 123 171 0.0 6 169 1 1 124 230 0.0 6 222 7 0 0 0 1 125 226 0.0 6 224 1 0 0 0 0 1 126 229 0.0 6 223 6 127 258 0.0 6 251 5 0 0 2 128 305 0.0 6 298 6 0 1 129 324 0.0 6 318 5 0 0 0 0 1 130 304 0.0 6 300 4 131 361 0.0 6 354 6 0 1 132 341 0.0 6 335 5 0 0 0 1 133 390 0.0 6 384 5 0 0 0 0 1 134 417 0.0 6 411 4 0 0 0 1 1 135 426 0.0 6 419 6 0 1 136 441 0.0 6 437 4 137 452 0.0 6 448 4 138 568 0.0 6 563 5 139 787 0.0 6 779 8 140 831 0.0 6 824 7 141 863 0.0 6 859 4 142 901 0.0 6 890 11 143 998 0.0 6 988 9 0 1 144 982 0.0 6 973 8 1 145 1008 0.0 6 997 11 146 1086 0.0 6 1083 3 147 1100 0.0 6 1092 8 148 1203 0.0 6 1189 10 0 0 1 2 1 149 1228 0.0 6 1217 8 0 1 1 1 150 1408 0.0 6 1391 13 1 1 2 151 1231 0.0 6 1218 9 0 1 2 1 152 1368 0.0 6 1352 11 0 0 2 1 2 153 1311 0.0 6 1296 12 0 2 0 0 1 154 1125 0.0 6 1117 7 1 155 1251 0.0 6 1240 9 0 0 2 156 1028 0.0 6 1023 4 1 157 1290 0.0 6 1277 11 1 0 1 158 1140 0.0 6 1128 9 1 1 0 1 159 1080 0.0 6 1072 6 1 0 0 1 160 1074 0.0 6 1065 9 161 1016 0.0 6 1006 7 1 0 0 0 2 162 872 0.0 6 861 8 1 1 0 1 163 933 0.0 6 929 4 164 910 0.0 6 902 4 0 1 1 1 1 165 886 0.0 6 879 5 1 1 166 1080 0.0 6 1071 7 1 0 0 1 167 858 0.0 6 852 6 168 1020 0.0 6 1014 5 0 1 169 939 0.0 6 935 4 170 916 0.0 6 907 7 0 1 1 171 994 0.0 6 987 7 172 988 0.0 6 985 3 173 999 0.0 6 993 6 174 899 0.0 6 893 4 0 0 2 175 949 0.0 6 948 0 0 0 0 0 1 176 1046 0.0 6 1039 4 0 0 1 2 177 682 0.0 6 679 2 1 178 753 0.0 6 750 2 1 179 580 0.0 6 575 5 180 557 0.0 6 556 0 1 181 517 0.0 6 516 1 182 524 0.0 6 519 4 0 1 183 750 0.0 6 747 2 0 0 0 0 1 184 958 0.0 6 953 4 0 0 1 185 795 0.0 6 793 2 186 538 0.0 6 537 1 187 539 0.0 6 532 5 1 1 188 522 0.0 6 520 1 0 0 0 0 1 189 502 0.0 6 500 2 190 434 0.0 6 432 2 191 474 0.0 6 472 2 192 351 0.0 6 348 3 193 325 0.0 6 323 1 1 194 311 0.0 6 309 2 195 253 0.0 6 251 2 196 366 0.0 6 364 2 197 313 0.0 6 313 198 242 0.0 6 240 2 199 231 0.0 6 230 1 200 204 0.0 6 204 201 177 0.0 6 177 202 184 0.0 6 184 203 154 0.0 6 154 204 140 0.0 6 140 205 151 0.0 6 151 206 124 0.0 6 123 1 207 104 0.0 6 102 2 208 103 0.0 6 103 209 104 0.0 6 103 0 0 0 0 0 1 210 75 0.0 6 75 211 79 0.0 6 78 1 212 92 0.0 6 92 213 80 0.0 6 79 1 214 99 0.0 6 98 1 215 64 0.0 6 64 216 61 0.0 6 61 217 52 0.0 6 52 218 35 0.0 6 35 219 36 0.0 6 35 1 220 27 0.0 6 27 221 37 0.0 6 37 222 22 0.0 6 22 223 40 0.0 6 40 224 21 0.0 6 21 225 17 0.0 6 17 226 15 0.0 6 15 227 11 0.0 6 10 1 228 16 0.0 6 15 1 229 11 0.0 6 11 230 6 0.0 6 6 231 7 0.0 6 7 232 4 0.0 6 4 233 5 0.0 6 5 234 6 0.0 6 6 235 4 0.0 6 4 236 6 0.0 6 6 238 2 0.0 6 2 239 3 0.0 6 3 240 2 0.0 6 2 241 1 0.0 6 1 243 1 0.0 6 1 244 1 0.0 6 1 245 1 0.0 6 1 248 2 0.0 6 2 265 1 0.0 6 1 278 3 0.0 6 3 279 54 0.0 6 53 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1306 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 49 1 0.0 6 0 1 55 7 0.0 6 7 56 1 0.0 6 1 61 3 0.0 6 3 63 2 0.0 6 2 71 1 0.0 6 1 72 7 0.0 6 7 73 10 0.0 6 10 74 15 0.0 6 15 75 14 0.0 6 14 76 7 0.0 6 7 77 17 0.0 6 15 0 1 0 1 78 9 0.0 6 9 79 17 0.0 6 17 80 14 0.0 6 14 81 10 0.0 6 10 82 12 0.0 6 12 83 16 0.0 6 16 84 14 0.0 6 14 85 10 0.0 6 10 86 9 0.0 6 9 87 6 0.0 6 6 88 9 0.0 6 9 89 10 0.0 6 10 90 11 0.0 6 11 91 10 0.0 6 10 92 17 0.0 6 17 93 11 0.0 6 11 94 10 0.0 6 10 95 6 0.0 6 6 96 8 0.0 6 8 97 13 0.0 6 13 98 7 0.0 6 7 99 7 0.0 6 7 100 12 0.0 6 12 101 5 0.0 6 4 0 0 0 1 102 15 0.0 6 15 103 6 0.0 6 6 104 17 0.0 6 16 0 0 0 1 105 4 0.0 6 4 106 3 0.0 6 3 107 10 0.0 6 10 108 5 0.0 6 5 109 16 0.0 6 16 110 41 0.0 6 41 111 7 0.0 6 7 112 11 0.0 6 11 113 9 0.0 6 9 114 6 0.0 6 6 115 9 0.0 6 9 116 11 0.0 6 11 117 10 0.0 6 10 118 7 0.0 6 7 119 7 0.0 6 7 120 10 0.0 6 10 121 8 0.0 6 8 122 11 0.0 6 11 123 9 0.0 6 9 124 15 0.0 6 15 125 9 0.0 6 9 126 9 0.0 6 9 127 6 0.0 6 6 128 10 0.0 6 10 129 7 0.0 6 7 130 13 0.0 6 13 131 15 0.0 6 15 132 13 0.0 6 13 133 11 0.0 6 11 134 20 0.0 6 20 135 8 0.0 6 8 136 16 0.0 6 16 137 12 0.0 6 12 138 18 0.0 6 18 139 10 0.0 6 10 140 11 0.0 6 11 141 13 0.0 6 13 142 13 0.0 6 13 143 8 0.0 6 8 144 5 0.0 6 5 145 12 0.0 6 12 146 12 0.0 6 12 147 7 0.0 6 7 148 8 0.0 6 8 149 15 0.0 6 15 150 8 0.0 6 8 151 11 0.0 6 10 0 1 152 11 0.0 6 11 153 10 0.0 6 10 154 9 0.0 6 8 0 0 0 1 155 12 0.0 6 12 156 9 0.0 6 9 157 10 0.0 6 10 158 13 0.0 6 13 159 10 0.0 6 10 160 6 0.0 6 6 161 14 0.0 6 14 162 8 0.0 6 8 163 13 0.0 6 13 164 8 0.0 6 8 165 12 0.0 6 12 166 9 0.0 6 9 167 22 0.0 6 22 168 14 0.0 6 14 169 11 0.0 6 11 170 10 0.0 6 10 171 8 0.0 6 8 172 15 0.0 6 15 173 6 0.0 6 6 174 7 0.0 6 7 175 7 0.0 6 7 176 8 0.0 6 8 177 5 0.0 6 5 178 6 0.0 6 6 179 8 0.0 6 8 180 6 0.0 6 6 181 6 0.0 6 6 182 6 0.0 6 6 183 4 0.0 6 4 184 5 0.0 6 5 185 3 0.0 6 3 186 6 0.0 6 6 187 7 0.0 6 7 188 5 0.0 6 5 189 11 0.0 6 11 190 2 0.0 6 2 191 5 0.0 6 5 192 4 0.0 6 4 193 4 0.0 6 4 194 3 0.0 6 3 195 7 0.0 6 7 196 3 0.0 6 3 197 5 0.0 6 5 198 5 0.0 6 5 199 1 0.0 6 1 200 2 0.0 6 2 201 4 0.0 6 4 203 3 0.0 6 3 205 3 0.0 6 3 207 2 0.0 6 2 208 7 0.0 6 7 209 1 0.0 6 1 210 1 0.0 6 1 211 1 0.0 6 1 214 1 0.0 6 1 216 2 0.0 6 2 218 2 0.0 6 2 219 1 0.0 6 1 220 1 0.0 6 1 221 1 0.0 6 1 224 2 0.0 6 2 226 2 0.0 6 1 0 0 0 1 227 1 0.0 6 1 228 1 0.0 6 1 230 1 0.0 6 1 233 1 0.0 6 1 235 1 0.0 6 1 277 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_01_36_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_01_263_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_01_36_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_01_263_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_02_37_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_02_264_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_02_37_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_02_264_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_03_38_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_03_265_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_03_38_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_03_265_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_04_39_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_04_266_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_04_39_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_04_266_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_05_40_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_05_267_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_05_40_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_05_267_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_06_41_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_06_268_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_06_41_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_06_268_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_15_42_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_15_269_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_15_42_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_15_269_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_16_43_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_16_270_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_16_43_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_16_270_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_17_44_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_17_271_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_17_44_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_17_271_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_18_45_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_18_272_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_18_45_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_18_272_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_19_46_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_19_273_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_19_46_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_19_273_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_20_47_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_04-Rev_20_274_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_20_47_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_04-Rev_20_274_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_01_48_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_01_275_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_01_48_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_01_275_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.91 s (168 µs/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 5,410 Read 1 with adapter: 5,410 (100.0%) Read 2 with adapter: 5,390 (99.6%) == Read fate breakdown == Pairs that were too short: 1 (0.0%) Pairs discarded as untrimmed: 20 (0.4%) Pairs written (passing filters): 5,389 (99.6%) Total basepairs processed: 2,201,316 bp Read 1: 1,195,842 bp Read 2: 1,005,474 bp Total written (filtered): 356,155 bp (16.2%) Read 1: 183,098 bp Read 2: 173,057 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 5137 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 84.5 0 0 1 9 1 0.0 2 1 20 6 0.0 6 6 21 2 0.0 6 1 1 22 1 0.0 6 1 23 2 0.0 6 2 24 2 0.0 6 2 27 2 0.0 6 2 28 1 0.0 6 1 29 2 0.0 6 2 36 1 0.0 6 0 1 37 2 0.0 6 2 38 1 0.0 6 1 40 2 0.0 6 2 42 1 0.0 6 0 0 0 0 0 1 44 1 0.0 6 1 59 1 0.0 6 1 63 2 0.0 6 2 65 1 0.0 6 1 66 2 0.0 6 2 68 1 0.0 6 1 69 1 0.0 6 1 71 1 0.0 6 1 74 1 0.0 6 1 75 1 0.0 6 1 76 1 0.0 6 1 77 1 0.0 6 1 79 1 0.0 6 1 80 1 0.0 6 1 83 1 0.0 6 1 84 1 0.0 6 1 85 1 0.0 6 1 86 1 0.0 6 1 88 1 0.0 6 1 89 1 0.0 6 1 90 2 0.0 6 2 95 1 0.0 6 1 97 1 0.0 6 1 100 1 0.0 6 1 101 1 0.0 6 1 102 4 0.0 6 4 105 4 0.0 6 4 106 2 0.0 6 2 107 1 0.0 6 1 108 3 0.0 6 3 109 5 0.0 6 5 110 1 0.0 6 1 111 4 0.0 6 4 112 4 0.0 6 4 113 5 0.0 6 5 114 4 0.0 6 4 115 3 0.0 6 3 117 4 0.0 6 4 118 3 0.0 6 2 0 0 0 0 1 119 2 0.0 6 1 0 0 0 0 1 121 4 0.0 6 4 122 3 0.0 6 3 124 5 0.0 6 5 125 2 0.0 6 2 126 2 0.0 6 2 127 6 0.0 6 6 129 4 0.0 6 4 130 3 0.0 6 3 131 4 0.0 6 4 132 4 0.0 6 4 133 6 0.0 6 6 134 1 0.0 6 1 135 7 0.0 6 7 136 6 0.0 6 6 137 1 0.0 6 1 138 4 0.0 6 4 139 7 0.0 6 7 140 9 0.0 6 9 141 56 0.0 6 55 1 142 101 0.0 6 101 143 109 0.0 6 109 144 94 0.0 6 93 1 145 116 0.0 6 116 146 112 0.0 6 111 1 147 112 0.0 6 112 148 120 0.0 6 120 149 98 0.0 6 98 150 96 0.0 6 95 1 151 127 0.0 6 127 152 88 0.0 6 88 153 64 0.0 6 64 154 68 0.0 6 67 1 155 66 0.0 6 66 156 53 0.0 6 53 157 43 0.0 6 43 158 46 0.0 6 46 159 48 0.0 6 48 160 67 0.0 6 67 161 69 0.0 6 69 162 57 0.0 6 55 2 163 56 0.0 6 56 164 59 0.0 6 59 165 47 0.0 6 47 166 41 0.0 6 41 167 37 0.0 6 37 168 41 0.0 6 41 169 38 0.0 6 38 170 40 0.0 6 40 171 34 0.0 6 34 172 43 0.0 6 43 173 35 0.0 6 35 174 35 0.0 6 35 175 27 0.0 6 26 1 176 34 0.0 6 33 0 0 0 1 177 30 0.0 6 30 178 37 0.0 6 37 179 63 0.0 6 63 180 35 0.0 6 34 1 181 20 0.0 6 20 182 29 0.0 6 28 1 183 30 0.0 6 29 1 184 27 0.0 6 27 185 31 0.0 6 31 186 26 0.0 6 26 187 32 0.0 6 32 188 23 0.0 6 23 189 21 0.0 6 20 1 190 17 0.0 6 17 191 24 0.0 6 22 2 192 27 0.0 6 27 193 24 0.0 6 23 0 0 0 0 1 194 19 0.0 6 19 195 32 0.0 6 32 196 32 0.0 6 32 197 37 0.0 6 37 198 30 0.0 6 29 1 199 29 0.0 6 29 200 21 0.0 6 21 201 23 0.0 6 23 202 26 0.0 6 26 203 28 0.0 6 28 204 22 0.0 6 21 1 205 23 0.0 6 22 1 206 32 0.0 6 32 207 37 0.0 6 36 1 208 27 0.0 6 27 209 27 0.0 6 27 210 15 0.0 6 14 1 211 27 0.0 6 27 212 17 0.0 6 17 213 21 0.0 6 21 214 23 0.0 6 23 215 27 0.0 6 27 216 30 0.0 6 28 2 217 21 0.0 6 21 218 25 0.0 6 23 2 219 23 0.0 6 21 2 220 24 0.0 6 23 1 221 23 0.0 6 23 222 23 0.0 6 23 223 14 0.0 6 13 1 224 20 0.0 6 19 1 225 19 0.0 6 19 226 18 0.0 6 18 227 23 0.0 6 22 1 228 14 0.0 6 13 1 229 18 0.0 6 18 230 20 0.0 6 20 231 18 0.0 6 18 232 16 0.0 6 16 233 14 0.0 6 13 1 234 28 0.0 6 27 1 235 20 0.0 6 20 236 16 0.0 6 15 1 237 20 0.0 6 18 2 238 13 0.0 6 12 1 239 12 0.0 6 12 240 16 0.0 6 16 241 22 0.0 6 22 242 14 0.0 6 14 243 17 0.0 6 16 1 244 19 0.0 6 18 1 245 10 0.0 6 9 1 246 14 0.0 6 14 247 9 0.0 6 9 248 11 0.0 6 11 249 12 0.0 6 12 250 11 0.0 6 11 251 14 0.0 6 12 1 1 252 12 0.0 6 12 253 14 0.0 6 13 1 254 11 0.0 6 10 1 255 12 0.0 6 12 256 7 0.0 6 7 257 8 0.0 6 8 258 9 0.0 6 9 259 9 0.0 6 9 260 8 0.0 6 8 261 7 0.0 6 7 262 12 0.0 6 12 263 22 0.0 6 21 1 264 6 0.0 6 5 1 265 8 0.0 6 7 1 266 10 0.0 6 10 267 12 0.0 6 12 268 10 0.0 6 10 269 7 0.0 6 6 1 270 4 0.0 6 4 271 16 0.0 6 16 272 16 0.0 6 16 273 15 0.0 6 14 1 274 12 0.0 6 10 2 275 13 0.0 6 13 276 20 0.0 6 19 1 277 30 0.0 6 30 278 21 0.0 6 20 0 0 0 0 0 1 279 52 0.0 6 49 3 280 478 0.0 6 455 23 281 4 0.0 6 3 1 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 273 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 6 21.1 1 0 6 62 8 0.0 6 6 2 71 6 0.0 6 6 72 4 0.0 6 4 73 4 0.0 6 4 74 5 0.0 6 5 75 6 0.0 6 6 76 11 0.0 6 11 77 7 0.0 6 7 78 3 0.0 6 3 79 9 0.0 6 9 80 5 0.0 6 5 81 10 0.0 6 9 1 82 6 0.0 6 6 83 5 0.0 6 5 84 2 0.0 6 2 85 5 0.0 6 5 86 2 0.0 6 2 87 3 0.0 6 3 88 10 0.0 6 10 89 1 0.0 6 1 90 6 0.0 6 6 91 10 0.0 6 10 92 2 0.0 6 2 93 3 0.0 6 3 94 4 0.0 6 4 96 6 0.0 6 6 97 3 0.0 6 3 98 1 0.0 6 1 99 3 0.0 6 3 100 2 0.0 6 2 101 4 0.0 6 4 102 6 0.0 6 6 103 1 0.0 6 1 104 2 0.0 6 2 106 4 0.0 6 4 107 2 0.0 6 2 109 4 0.0 6 4 110 3 0.0 6 3 111 1 0.0 6 1 112 4 0.0 6 4 113 1 0.0 6 1 114 1 0.0 6 1 115 1 0.0 6 1 116 3 0.0 6 2 0 0 0 0 1 117 12 0.0 6 1 0 0 0 0 10 1 118 1 0.0 6 1 120 2 0.0 6 2 121 2 0.0 6 2 125 2 0.0 6 2 126 1 0.0 6 1 127 1 0.0 6 1 129 2 0.0 6 2 133 2 0.0 6 2 134 1 0.0 6 1 135 2 0.0 6 2 136 2 0.0 6 2 138 3 0.0 6 3 140 1 0.0 6 1 142 2 0.0 6 2 143 2 0.0 6 2 145 1 0.0 6 1 146 2 0.0 6 2 147 2 0.0 6 2 150 1 0.0 6 1 154 1 0.0 6 1 158 2 0.0 6 2 159 1 0.0 6 1 160 1 0.0 6 1 161 1 0.0 6 1 162 1 0.0 6 1 166 1 0.0 6 1 169 3 0.0 6 3 170 1 0.0 6 1 171 1 0.0 6 1 173 2 0.0 6 2 174 1 0.0 6 1 177 2 0.0 6 2 183 2 0.0 6 2 197 1 0.0 6 1 199 2 0.0 6 2 205 1 0.0 6 1 211 1 0.0 6 1 212 1 0.0 6 1 214 2 0.0 6 2 218 1 0.0 6 1 221 1 0.0 6 1 228 1 0.0 6 1 230 1 0.0 6 1 234 1 0.0 6 1 256 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 5186 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.8% G: 0.1% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 0.3 2 1 12 1 0.0 3 1 13 3 0.0 3 2 1 17 1 0.0 5 0 0 1 18 1 0.0 5 1 19 1 0.0 5 1 20 6 0.0 6 5 1 21 3 0.0 6 3 22 1 0.0 6 1 23 2 0.0 6 2 24 1 0.0 6 1 25 1 0.0 6 1 30 2 0.0 6 2 33 1 0.0 6 0 1 35 1 0.0 6 1 36 1 0.0 6 1 37 1 0.0 6 1 38 3 0.0 6 3 39 2 0.0 6 1 0 0 0 0 0 1 41 1 0.0 6 1 45 1 0.0 6 1 49 1 0.0 6 1 50 1 0.0 6 1 51 2 0.0 6 2 53 2 0.0 6 1 1 54 1 0.0 6 1 55 1 0.0 6 0 1 56 1 0.0 6 1 57 2 0.0 6 2 58 1 0.0 6 1 59 1 0.0 6 1 61 1 0.0 6 1 62 4 0.0 6 4 63 1 0.0 6 1 64 3 0.0 6 3 65 1 0.0 6 1 66 2 0.0 6 2 67 1 0.0 6 1 68 3 0.0 6 3 69 9 0.0 6 8 1 70 8 0.0 6 8 71 7 0.0 6 6 1 72 2 0.0 6 2 73 3 0.0 6 3 74 3 0.0 6 3 75 2 0.0 6 2 76 3 0.0 6 3 77 4 0.0 6 4 78 2 0.0 6 2 79 4 0.0 6 4 80 4 0.0 6 4 81 3 0.0 6 2 1 82 4 0.0 6 3 1 83 5 0.0 6 5 84 2 0.0 6 2 85 4 0.0 6 4 86 3 0.0 6 3 87 1 0.0 6 1 88 4 0.0 6 3 0 1 89 1 0.0 6 1 90 8 0.0 6 8 91 2 0.0 6 2 92 8 0.0 6 5 3 93 2 0.0 6 2 94 8 0.0 6 7 1 95 6 0.0 6 4 2 96 6 0.0 6 6 97 7 0.0 6 7 98 8 0.0 6 8 99 3 0.0 6 2 1 100 6 0.0 6 6 101 6 0.0 6 6 102 10 0.0 6 10 103 5 0.0 6 5 104 7 0.0 6 7 105 13 0.0 6 13 106 11 0.0 6 10 1 107 6 0.0 6 6 108 4 0.0 6 4 109 8 0.0 6 8 110 9 0.0 6 9 111 5 0.0 6 4 1 112 7 0.0 6 7 113 9 0.0 6 8 1 114 7 0.0 6 7 115 14 0.0 6 14 116 15 0.0 6 14 1 117 21 0.0 6 21 118 6 0.0 6 6 119 13 0.0 6 13 120 12 0.0 6 12 121 18 0.0 6 18 122 11 0.0 6 9 2 123 14 0.0 6 13 1 124 12 0.0 6 12 125 19 0.0 6 19 126 27 0.0 6 27 127 23 0.0 6 22 1 128 29 0.0 6 29 129 20 0.0 6 20 130 25 0.0 6 23 2 131 31 0.0 6 31 132 28 0.0 6 27 1 133 23 0.0 6 23 134 38 0.0 6 37 1 135 33 0.0 6 33 136 31 0.0 6 31 137 51 0.0 6 51 138 40 0.0 6 40 139 56 0.0 6 56 140 94 0.0 6 93 1 141 106 0.0 6 105 1 142 116 0.0 6 116 143 147 0.0 6 146 1 144 147 0.0 6 147 145 120 0.0 6 119 1 146 151 0.0 6 149 2 147 110 0.0 6 109 1 148 98 0.0 6 97 1 149 153 0.0 6 151 2 150 112 0.0 6 111 1 151 80 0.0 6 76 4 152 149 0.0 6 148 1 153 97 0.0 6 95 1 1 154 102 0.0 6 99 3 155 88 0.0 6 87 1 156 94 0.0 6 92 2 157 105 0.0 6 105 158 81 0.0 6 79 2 159 89 0.0 6 89 160 89 0.0 6 87 2 161 61 0.0 6 61 162 74 0.0 6 72 2 163 61 0.0 6 59 2 164 53 0.0 6 52 1 165 52 0.0 6 51 0 0 1 166 40 0.0 6 39 1 167 59 0.0 6 59 168 60 0.0 6 58 2 169 47 0.0 6 46 1 170 56 0.0 6 55 1 171 40 0.0 6 40 172 42 0.0 6 40 2 173 49 0.0 6 49 174 46 0.0 6 46 175 49 0.0 6 48 1 176 42 0.0 6 39 3 177 44 0.0 6 42 1 0 1 178 36 0.0 6 36 179 29 0.0 6 28 1 180 22 0.0 6 21 1 181 27 0.0 6 27 182 28 0.0 6 28 183 32 0.0 6 31 1 184 19 0.0 6 19 185 33 0.0 6 33 186 47 0.0 6 46 1 187 46 0.0 6 46 188 56 0.0 6 54 2 189 33 0.0 6 32 1 190 43 0.0 6 42 1 191 33 0.0 6 32 1 192 33 0.0 6 33 193 28 0.0 6 26 2 194 27 0.0 6 26 1 195 35 0.0 6 34 1 196 33 0.0 6 32 1 197 16 0.0 6 15 1 198 40 0.0 6 36 4 199 44 0.0 6 42 2 200 17 0.0 6 14 3 201 14 0.0 6 14 202 15 0.0 6 14 1 203 16 0.0 6 16 204 27 0.0 6 26 1 205 11 0.0 6 11 206 13 0.0 6 12 1 207 9 0.0 6 9 208 3 0.0 6 3 209 4 0.0 6 4 210 8 0.0 6 7 1 211 10 0.0 6 9 1 212 9 0.0 6 9 213 4 0.0 6 3 1 214 6 0.0 6 6 215 9 0.0 6 6 3 216 19 0.0 6 19 217 7 0.0 6 7 218 10 0.0 6 10 219 9 0.0 6 9 220 3 0.0 6 2 1 221 3 0.0 6 3 222 9 0.0 6 9 223 6 0.0 6 5 1 224 7 0.0 6 6 1 225 12 0.0 6 12 226 5 0.0 6 3 2 227 1 0.0 6 1 228 2 0.0 6 2 229 3 0.0 6 3 230 5 0.0 6 5 231 6 0.0 6 6 232 5 0.0 6 4 1 233 2 0.0 6 2 234 5 0.0 6 5 235 1 0.0 6 1 237 1 0.0 6 1 238 1 0.0 6 1 240 4 0.0 6 3 1 242 3 0.0 6 2 1 243 4 0.0 6 4 244 2 0.0 6 2 246 1 0.0 6 1 247 1 0.0 6 1 250 1 0.0 6 1 253 1 0.0 6 1 279 1 0.0 6 1 280 1 0.0 6 1 281 3 0.0 6 3 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 204 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 47 2 0.0 6 0 2 51 1 0.0 6 1 69 2 0.0 6 2 70 7 0.0 6 7 71 6 0.0 6 6 72 8 0.0 6 8 73 5 0.0 6 5 74 3 0.0 6 3 75 5 0.0 6 4 0 0 0 1 76 3 0.0 6 3 77 6 0.0 6 6 78 10 0.0 6 10 79 6 0.0 6 6 80 11 0.0 6 11 81 7 0.0 6 7 83 2 0.0 6 2 84 4 0.0 6 4 85 1 0.0 6 1 86 4 0.0 6 4 87 6 0.0 6 6 88 1 0.0 6 1 89 5 0.0 6 5 90 3 0.0 6 3 91 2 0.0 6 2 92 2 0.0 6 2 93 4 0.0 6 4 95 3 0.0 6 2 0 0 0 1 96 2 0.0 6 2 97 2 0.0 6 2 98 1 0.0 6 1 99 3 0.0 6 3 100 3 0.0 6 3 101 3 0.0 6 3 102 4 0.0 6 4 103 1 0.0 6 1 104 1 0.0 6 1 105 1 0.0 6 1 106 1 0.0 6 1 107 1 0.0 6 1 108 10 0.0 6 10 109 1 0.0 6 1 110 2 0.0 6 2 111 2 0.0 6 2 112 2 0.0 6 2 113 2 0.0 6 2 114 4 0.0 6 4 115 1 0.0 6 1 117 1 0.0 6 1 119 2 0.0 6 2 121 2 0.0 6 2 122 1 0.0 6 1 123 1 0.0 6 1 124 1 0.0 6 1 125 1 0.0 6 1 127 1 0.0 6 1 128 3 0.0 6 3 129 1 0.0 6 1 132 1 0.0 6 1 133 1 0.0 6 1 134 1 0.0 6 1 136 2 0.0 6 2 137 1 0.0 6 1 139 2 0.0 6 2 140 2 0.0 6 2 141 2 0.0 6 2 146 1 0.0 6 1 150 1 0.0 6 1 155 1 0.0 6 0 0 0 0 1 156 1 0.0 6 0 0 0 0 1 158 1 0.0 6 0 1 159 1 0.0 6 1 162 1 0.0 6 1 173 1 0.0 6 1 174 2 0.0 6 2 178 1 0.0 6 1 190 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_02_49_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_02_276_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_02_49_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_02_276_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.68 s (25 µs/read; 2.43 M reads/minute). === Summary === Total read pairs processed: 67,895 Read 1 with adapter: 67,895 (100.0%) Read 2 with adapter: 67,895 (100.0%) == Read fate breakdown == Pairs that were too short: 1 (0.0%) Pairs discarded as untrimmed: 0 (0.0%) Pairs written (passing filters): 67,894 (100.0%) Total basepairs processed: 33,053,392 bp Read 1: 18,956,114 bp Read 2: 14,097,278 bp Total written (filtered): 4,214,790 bp (12.8%) Read 1: 2,108,422 bp Read 2: 2,106,368 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 67819 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1060.9 0 2 4 1 265.2 1 1 6 3 16.6 1 0 0 3 9 1 0.3 2 0 1 14 1 0.0 4 1 15 2 0.0 4 2 16 2 0.0 4 2 17 2 0.0 5 1 0 1 18 1 0.0 5 0 1 20 16 0.0 6 15 0 1 21 9 0.0 6 9 22 10 0.0 6 9 1 23 4 0.0 6 3 1 24 4 0.0 6 4 25 10 0.0 6 9 1 26 4 0.0 6 4 27 4 0.0 6 4 28 3 0.0 6 3 29 4 0.0 6 4 30 4 0.0 6 4 31 2 0.0 6 2 32 1 0.0 6 1 33 1 0.0 6 1 34 2 0.0 6 1 1 35 2 0.0 6 2 37 1 0.0 6 1 38 3 0.0 6 2 1 40 4 0.0 6 3 1 41 2 0.0 6 2 42 2 0.0 6 2 44 2 0.0 6 2 45 12 0.0 6 11 0 1 46 3 0.0 6 3 48 1 0.0 6 1 49 1 0.0 6 1 50 1 0.0 6 1 52 1 0.0 6 1 53 6 0.0 6 6 54 2 0.0 6 2 56 5 0.0 6 5 58 1 0.0 6 1 59 3 0.0 6 2 0 1 60 6 0.0 6 6 61 1 0.0 6 1 62 9 0.0 6 9 63 3 0.0 6 3 64 3 0.0 6 3 65 4 0.0 6 3 0 1 66 3 0.0 6 1 1 1 67 1 0.0 6 1 68 5 0.0 6 5 69 3 0.0 6 3 70 2 0.0 6 2 71 2 0.0 6 1 0 0 1 72 2 0.0 6 2 73 2 0.0 6 2 74 3 0.0 6 3 75 1 0.0 6 0 1 76 1 0.0 6 1 77 1 0.0 6 1 78 3 0.0 6 2 1 79 2 0.0 6 2 80 2 0.0 6 2 81 2 0.0 6 2 82 1 0.0 6 1 83 3 0.0 6 3 84 2 0.0 6 2 85 1 0.0 6 1 86 17 0.0 6 16 1 87 3 0.0 6 3 88 1 0.0 6 1 89 5 0.0 6 4 1 90 4 0.0 6 4 91 4 0.0 6 3 1 92 6 0.0 6 6 93 3 0.0 6 3 94 2 0.0 6 2 95 1 0.0 6 1 97 2 0.0 6 1 1 98 7 0.0 6 7 99 1 0.0 6 1 100 3 0.0 6 3 101 20 0.0 6 19 0 1 102 6 0.0 6 6 103 7 0.0 6 7 104 2 0.0 6 2 105 6 0.0 6 6 106 4 0.0 6 4 107 10 0.0 6 10 108 21 0.0 6 20 1 109 5 0.0 6 5 110 3 0.0 6 3 111 6 0.0 6 6 112 10 0.0 6 9 1 113 10 0.0 6 10 114 5 0.0 6 5 115 9 0.0 6 7 1 1 116 8 0.0 6 7 1 117 2 0.0 6 2 118 4 0.0 6 3 0 0 0 0 1 119 10 0.0 6 9 1 120 9 0.0 6 8 1 121 11 0.0 6 11 122 22 0.0 6 20 2 123 13 0.0 6 13 124 11 0.0 6 9 2 125 22 0.0 6 20 2 126 26 0.0 6 25 1 127 14 0.0 6 14 128 16 0.0 6 16 129 9 0.0 6 9 130 14 0.0 6 13 1 131 12 0.0 6 12 132 15 0.0 6 15 133 7 0.0 6 5 2 134 17 0.0 6 16 1 135 23 0.0 6 22 1 136 18 0.0 6 18 137 47 0.0 6 44 3 138 45 0.0 6 42 3 139 16 0.0 6 15 1 140 23 0.0 6 22 1 141 33 0.0 6 32 1 142 99 0.0 6 98 1 143 105 0.0 6 100 5 144 82 0.0 6 80 2 145 69 0.0 6 67 2 146 74 0.0 6 73 1 147 86 0.0 6 85 1 148 138 0.0 6 137 1 149 84 0.0 6 80 4 150 95 0.0 6 90 4 0 0 1 151 71 0.0 6 68 3 152 49 0.0 6 48 1 153 62 0.0 6 62 154 36 0.0 6 36 155 45 0.0 6 45 156 57 0.0 6 56 1 157 41 0.0 6 40 1 158 56 0.0 6 55 0 1 159 59 0.0 6 59 160 64 0.0 6 64 161 65 0.0 6 63 1 1 162 66 0.0 6 65 1 163 54 0.0 6 54 164 67 0.0 6 64 2 1 165 68 0.0 6 67 1 166 57 0.0 6 53 4 167 46 0.0 6 46 168 60 0.0 6 56 4 169 52 0.0 6 52 170 79 0.0 6 78 1 171 65 0.0 6 62 2 1 172 82 0.0 6 81 1 173 82 0.0 6 80 2 174 85 0.0 6 83 2 175 95 0.0 6 91 3 1 176 89 0.0 6 86 3 177 118 0.0 6 112 6 178 79 0.0 6 76 3 179 110 0.0 6 109 1 180 164 0.0 6 161 3 181 142 0.0 6 138 4 182 115 0.0 6 110 5 183 117 0.0 6 109 7 0 1 184 163 0.0 6 153 10 185 175 0.0 6 172 3 186 269 0.0 6 266 3 187 142 0.0 6 137 5 188 140 0.0 6 139 0 1 189 129 0.0 6 125 4 190 137 0.0 6 134 3 191 176 0.0 6 169 6 0 1 192 138 0.0 6 131 7 193 196 0.0 6 187 8 1 194 172 0.0 6 168 4 195 202 0.0 6 195 7 196 281 0.0 6 269 12 197 246 0.0 6 241 5 198 359 0.0 6 345 13 1 199 354 0.0 6 341 12 0 1 200 243 0.0 6 230 13 201 264 0.0 6 258 6 202 315 0.0 6 312 3 203 358 0.0 6 350 8 204 280 0.0 6 270 10 205 284 0.0 6 279 3 2 206 239 0.0 6 236 3 207 433 0.0 6 421 11 0 0 1 208 323 0.0 6 315 8 209 339 0.0 6 328 11 210 256 0.0 6 247 9 211 194 0.0 6 187 7 212 332 0.0 6 322 10 213 350 0.0 6 338 12 214 325 0.0 6 314 11 215 345 0.0 6 334 9 2 216 370 0.0 6 359 10 1 217 482 0.0 6 470 10 2 218 347 0.0 6 336 9 1 1 219 325 0.0 6 311 13 1 220 343 0.0 6 336 7 221 404 0.0 6 394 9 1 222 433 0.0 6 420 12 1 223 363 0.0 6 359 4 224 274 0.0 6 269 5 225 327 0.0 6 319 8 226 598 0.0 6 589 9 227 528 0.0 6 513 15 228 540 0.0 6 518 21 0 0 1 229 423 0.0 6 415 8 230 508 0.0 6 498 10 231 339 0.0 6 325 14 232 466 0.0 6 453 13 233 504 0.0 6 487 17 234 474 0.0 6 458 14 2 235 385 0.0 6 372 13 236 347 0.0 6 342 5 237 398 0.0 6 394 4 238 413 0.0 6 405 7 1 239 420 0.0 6 406 13 0 0 0 0 1 240 552 0.0 6 535 17 241 545 0.0 6 529 16 242 471 0.0 6 462 9 243 443 0.0 6 436 7 244 450 0.0 6 440 10 245 564 0.0 6 548 15 0 1 246 322 0.0 6 317 5 247 340 0.0 6 330 10 248 274 0.0 6 257 16 1 249 587 0.0 6 576 10 1 250 391 0.0 6 383 6 2 251 449 0.0 6 437 12 252 419 0.0 6 411 6 2 253 349 0.0 6 335 14 254 806 0.0 6 793 13 255 523 0.0 6 509 14 256 351 0.0 6 336 14 0 0 1 257 469 0.0 6 450 19 258 778 0.0 6 758 19 1 259 748 0.0 6 724 23 1 260 747 0.0 6 728 19 261 377 0.0 6 359 17 1 262 491 0.0 6 476 15 263 988 0.0 6 967 21 264 509 0.0 6 499 10 265 451 0.0 6 444 7 266 471 0.0 6 462 9 267 1110 0.0 6 1095 15 268 520 0.0 6 508 12 269 871 0.0 6 845 25 1 270 706 0.0 6 686 18 2 271 920 0.0 6 893 27 272 1236 0.0 6 1213 22 1 273 748 0.0 6 727 20 1 274 830 0.0 6 806 23 1 275 991 0.0 6 965 25 1 276 1436 0.0 6 1396 37 3 277 1872 0.0 6 1830 42 278 1880 0.0 6 1834 45 1 279 3790 0.0 6 3659 126 4 1 280 14603 0.0 6 14170 427 4 0 0 0 2 281 92 0.0 6 81 11 282 2 0.0 6 2 284 1 0.0 6 0 0 0 0 1 286 1 0.0 6 1 289 1 0.0 6 1 290 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 76 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 7 265.2 1 0 7 71 3 0.0 6 3 73 1 0.0 6 1 74 1 0.0 6 1 76 1 0.0 6 1 78 1 0.0 6 1 80 1 0.0 6 1 81 1 0.0 6 1 82 1 0.0 6 1 86 1 0.0 6 1 87 1 0.0 6 1 89 1 0.0 6 1 90 1 0.0 6 1 91 1 0.0 6 1 92 2 0.0 6 2 96 1 0.0 6 1 103 1 0.0 6 1 108 1 0.0 6 1 112 2 0.0 6 2 114 2 0.0 6 2 116 1 0.0 6 1 117 1 0.0 6 1 125 3 0.0 6 3 127 1 0.0 6 1 131 2 0.0 6 2 133 1 0.0 6 1 139 1 0.0 6 1 143 1 0.0 6 1 144 1 0.0 6 1 145 1 0.0 6 1 149 1 0.0 6 1 150 2 0.0 6 2 153 2 0.0 6 2 159 2 0.0 6 2 162 1 0.0 6 1 164 2 0.0 6 2 165 1 0.0 6 1 167 1 0.0 6 1 169 1 0.0 6 1 174 1 0.0 6 1 176 1 0.0 6 1 179 1 0.0 6 1 182 1 0.0 6 1 184 1 0.0 6 1 190 1 0.0 6 1 192 1 0.0 6 1 193 1 0.0 6 1 199 1 0.0 6 1 202 2 0.0 6 2 206 1 0.0 6 1 210 1 0.0 6 1 213 1 0.0 6 1 217 1 0.0 6 1 228 1 0.0 6 1 233 1 0.0 6 1 234 1 0.0 6 1 253 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 67741 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 4.1 2 1 10 7 0.1 3 5 1 0 1 11 4 0.0 3 4 12 10 0.0 3 7 3 13 10 0.0 3 9 1 14 10 0.0 4 10 15 2 0.0 4 2 16 8 0.0 4 8 17 29 0.0 5 27 1 1 18 21 0.0 5 16 5 19 12 0.0 5 10 2 20 32 0.0 6 30 2 21 55 0.0 6 54 1 22 45 0.0 6 44 1 23 29 0.0 6 26 3 24 28 0.0 6 26 2 25 18 0.0 6 17 1 26 28 0.0 6 25 2 1 27 14 0.0 6 11 3 28 16 0.0 6 14 2 29 16 0.0 6 13 3 30 13 0.0 6 10 2 1 31 8 0.0 6 7 1 32 10 0.0 6 10 33 8 0.0 6 7 1 34 11 0.0 6 10 1 35 8 0.0 6 6 2 36 3 0.0 6 3 37 16 0.0 6 14 2 38 16 0.0 6 15 1 39 15 0.0 6 13 2 40 13 0.0 6 12 1 41 10 0.0 6 8 2 42 6 0.0 6 6 43 11 0.0 6 11 44 26 0.0 6 25 1 45 18 0.0 6 12 5 0 0 1 46 36 0.0 6 35 1 47 31 0.0 6 29 2 48 21 0.0 6 20 1 49 17 0.0 6 16 1 50 21 0.0 6 20 1 51 14 0.0 6 13 1 52 23 0.0 6 21 2 53 20 0.0 6 15 4 1 54 24 0.0 6 23 1 55 24 0.0 6 21 3 56 22 0.0 6 19 3 57 31 0.0 6 27 4 58 24 0.0 6 23 1 59 9 0.0 6 9 60 18 0.0 6 17 1 61 10 0.0 6 9 1 62 9 0.0 6 9 63 15 0.0 6 15 64 13 0.0 6 12 1 65 3 0.0 6 3 66 15 0.0 6 15 67 57 0.0 6 51 5 0 0 1 68 100 0.0 6 96 4 69 26 0.0 6 25 1 70 51 0.0 6 46 5 71 28 0.0 6 23 5 72 73 0.0 6 64 7 0 0 2 73 5 0.0 6 5 74 8 0.0 6 8 75 11 0.0 6 8 3 76 29 0.0 6 26 1 2 77 29 0.0 6 26 3 78 33 0.0 6 32 1 79 28 0.0 6 25 3 80 26 0.0 6 23 2 1 81 14 0.0 6 13 1 82 42 0.0 6 41 1 83 57 0.0 6 53 4 84 67 0.0 6 58 7 1 0 1 85 50 0.0 6 46 3 1 86 45 0.0 6 41 4 87 64 0.0 6 58 6 88 29 0.0 6 24 5 89 42 0.0 6 37 3 1 0 1 90 37 0.0 6 37 91 35 0.0 6 33 2 92 30 0.0 6 28 1 1 93 44 0.0 6 39 5 94 119 0.0 6 110 8 1 95 44 0.0 6 40 4 96 47 0.0 6 45 2 97 40 0.0 6 39 1 98 33 0.0 6 31 2 99 65 0.0 6 63 2 100 42 0.0 6 41 1 101 44 0.0 6 42 2 102 77 0.0 6 70 7 103 74 0.0 6 71 3 104 88 0.0 6 81 5 1 1 105 123 0.0 6 111 10 2 106 55 0.0 6 50 5 107 78 0.0 6 75 2 0 0 1 108 55 0.0 6 53 2 109 46 0.0 6 43 3 110 50 0.0 6 46 4 111 47 0.0 6 44 3 112 34 0.0 6 33 1 113 48 0.0 6 44 4 114 83 0.0 6 79 4 115 50 0.0 6 48 2 116 69 0.0 6 67 2 117 42 0.0 6 39 3 118 60 0.0 6 57 3 119 96 0.0 6 90 6 120 122 0.0 6 114 8 121 97 0.0 6 92 5 122 126 0.0 6 121 4 0 0 0 1 123 109 0.0 6 100 8 1 124 178 0.0 6 168 10 125 112 0.0 6 106 5 1 126 100 0.0 6 93 7 127 110 0.0 6 106 4 128 91 0.0 6 87 4 129 105 0.0 6 102 3 130 85 0.0 6 80 3 2 131 166 0.0 6 162 4 132 291 0.0 6 277 14 133 213 0.0 6 205 6 1 1 134 289 0.0 6 269 19 0 0 1 135 422 0.0 6 402 19 0 0 1 136 202 0.0 6 191 11 137 302 0.0 6 295 7 138 188 0.0 6 184 3 0 0 0 1 139 250 0.0 6 243 7 140 356 0.0 6 339 17 141 359 0.0 6 348 11 142 450 0.0 6 432 18 143 374 0.0 6 363 9 1 0 0 1 144 601 0.0 6 584 15 2 145 344 0.0 6 333 10 1 146 275 0.0 6 266 8 0 0 1 147 216 0.0 6 214 1 1 148 282 0.0 6 273 9 149 448 0.0 6 428 19 1 150 728 0.0 6 709 18 1 151 478 0.0 6 461 15 2 152 735 0.0 6 709 21 3 1 1 153 517 0.0 6 505 12 154 584 0.0 6 562 19 1 1 1 155 434 0.0 6 418 14 1 1 156 454 0.0 6 437 16 1 157 631 0.0 6 613 16 2 158 703 0.0 6 675 27 1 159 361 0.0 6 343 16 0 0 1 1 160 577 0.0 6 553 24 161 428 0.0 6 420 8 162 393 0.0 6 381 11 1 163 491 0.0 6 478 12 1 164 501 0.0 6 482 17 2 165 564 0.0 6 545 19 166 686 0.0 6 661 20 4 0 0 1 167 394 0.0 6 380 14 168 1041 0.0 6 1008 30 1 1 1 169 303 0.0 6 297 6 170 418 0.0 6 405 13 171 874 0.0 6 844 30 172 330 0.0 6 320 10 173 369 0.0 6 356 11 1 1 174 594 0.0 6 576 16 1 1 175 1091 0.0 6 1048 39 1 0 3 176 564 0.0 6 543 20 1 177 972 0.0 6 939 31 1 0 0 1 178 672 0.0 6 641 29 1 0 1 179 954 0.0 6 934 19 1 180 716 0.0 6 698 16 1 0 0 0 1 181 1316 0.0 6 1289 24 0 0 3 182 329 0.0 6 315 12 2 183 837 0.0 6 825 12 184 625 0.0 6 612 11 1 0 1 185 765 0.0 6 730 33 2 186 1742 0.0 6 1701 39 2 187 1204 0.0 6 1171 32 1 188 1303 0.0 6 1262 36 4 1 189 1193 0.0 6 1155 35 1 0 2 190 2135 0.0 6 2071 61 2 0 0 0 1 191 1763 0.0 6 1712 49 1 1 192 1351 0.0 6 1307 40 3 0 1 193 850 0.0 6 810 36 2 2 194 1204 0.0 6 1160 38 5 0 1 195 1246 0.0 6 1199 45 1 1 196 3277 0.0 6 3192 81 2 0 2 197 1052 0.0 6 1008 42 2 198 1667 0.0 6 1606 58 1 0 2 199 2488 0.0 6 2423 64 1 200 821 0.0 6 796 23 2 201 1194 0.0 6 1167 25 1 0 1 202 623 0.0 6 609 13 1 203 440 0.0 6 429 11 204 541 0.0 6 529 12 205 218 0.0 6 211 7 206 220 0.0 6 216 4 207 156 0.0 6 148 8 208 353 0.0 6 344 8 1 209 238 0.0 6 230 8 210 271 0.0 6 261 9 1 211 269 0.0 6 257 12 212 379 0.0 6 372 7 213 136 0.0 6 127 8 1 214 236 0.0 6 225 10 0 0 1 215 378 0.0 6 363 15 216 529 0.0 6 510 18 0 0 1 217 311 0.0 6 296 14 1 218 353 0.0 6 341 11 0 0 1 219 283 0.0 6 274 9 220 172 0.0 6 166 5 1 221 243 0.0 6 234 9 222 444 0.0 6 431 12 0 0 0 1 223 661 0.0 6 642 18 0 0 0 1 224 155 0.0 6 149 6 225 272 0.0 6 268 4 226 154 0.0 6 149 4 0 0 1 227 154 0.0 6 150 4 228 158 0.0 6 148 10 229 184 0.0 6 178 6 230 132 0.0 6 127 4 1 231 79 0.0 6 75 3 0 1 232 243 0.0 6 234 9 233 189 0.0 6 182 7 234 33 0.0 6 33 235 36 0.0 6 32 4 236 11 0.0 6 11 237 25 0.0 6 25 238 10 0.0 6 10 239 25 0.0 6 23 2 240 67 0.0 6 66 1 241 67 0.0 6 60 7 242 107 0.0 6 103 4 243 90 0.0 6 89 1 244 60 0.0 6 60 245 13 0.0 6 11 2 246 19 0.0 6 18 1 247 6 0.0 6 6 248 22 0.0 6 22 249 16 0.0 6 15 1 250 9 0.0 6 9 251 5 0.0 6 5 252 2 0.0 6 2 253 5 0.0 6 5 254 9 0.0 6 9 255 2 0.0 6 2 256 1 0.0 6 1 257 1 0.0 6 1 259 1 0.0 6 1 260 1 0.0 6 1 277 1 0.0 6 1 278 4 0.0 6 4 279 37 0.0 6 37 280 43 0.0 6 40 3 281 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 154 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 3 74 1060.9 0 74 53 2 0.0 6 0 2 71 1 0.0 6 1 72 2 0.0 6 2 74 1 0.0 6 1 75 3 0.0 6 3 76 1 0.0 6 1 79 2 0.0 6 2 82 1 0.0 6 1 84 1 0.0 6 1 85 1 0.0 6 1 87 2 0.0 6 2 91 2 0.0 6 2 94 2 0.0 6 2 102 1 0.0 6 1 103 3 0.0 6 3 108 2 0.0 6 2 109 3 0.0 6 3 111 1 0.0 6 1 113 1 0.0 6 1 114 1 0.0 6 1 115 1 0.0 6 1 123 1 0.0 6 1 124 1 0.0 6 1 125 1 0.0 6 1 126 1 0.0 6 1 127 1 0.0 6 1 133 2 0.0 6 2 134 1 0.0 6 1 135 3 0.0 6 3 137 2 0.0 6 2 139 2 0.0 6 2 141 1 0.0 6 1 142 3 0.0 6 3 144 1 0.0 6 1 146 1 0.0 6 1 147 1 0.0 6 1 151 2 0.0 6 2 152 1 0.0 6 1 153 4 0.0 6 4 162 2 0.0 6 2 168 1 0.0 6 1 170 1 0.0 6 1 171 1 0.0 6 1 173 2 0.0 6 2 175 1 0.0 6 1 176 1 0.0 6 1 181 1 0.0 6 1 182 1 0.0 6 1 185 1 0.0 6 1 186 1 0.0 6 1 187 1 0.0 6 1 188 1 0.0 6 1 207 1 0.0 6 1 211 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_03_50_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_03_277_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_03_50_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_03_277_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.76 s (20 µs/read; 2.98 M reads/minute). === Summary === Total read pairs processed: 87,655 Read 1 with adapter: 87,654 (100.0%) Read 2 with adapter: 87,588 (99.9%) == Read fate breakdown == Pairs that were too short: 17 (0.0%) Pairs discarded as untrimmed: 68 (0.1%) Pairs written (passing filters): 87,570 (99.9%) Total basepairs processed: 42,508,910 bp Read 1: 24,009,191 bp Read 2: 18,499,719 bp Total written (filtered): 5,570,561 bp (13.1%) Read 1: 2,755,116 bp Read 2: 2,815,445 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 86807 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 1369.6 0 1 3 4 1 342.4 1 1 6 2 21.4 1 1 0 1 7 5 5.4 2 4 0 0 1 8 1 1.3 2 0 0 0 1 9 3 0.3 2 2 1 10 1 0.1 3 1 11 1 0.0 3 1 14 1 0.0 4 1 15 5 0.0 4 4 1 16 1 0.0 4 1 17 2 0.0 5 2 19 1 0.0 5 0 1 20 53 0.0 6 52 1 21 33 0.0 6 31 2 22 31 0.0 6 29 2 23 15 0.0 6 13 2 24 8 0.0 6 8 25 16 0.0 6 14 2 26 14 0.0 6 13 1 27 10 0.0 6 10 28 11 0.0 6 10 1 30 4 0.0 6 4 31 3 0.0 6 3 32 3 0.0 6 1 2 33 1 0.0 6 0 1 34 3 0.0 6 2 1 35 2 0.0 6 2 36 2 0.0 6 2 37 2 0.0 6 2 38 4 0.0 6 4 39 2 0.0 6 1 1 40 8 0.0 6 8 41 1 0.0 6 1 42 9 0.0 6 9 43 1 0.0 6 1 44 5 0.0 6 5 45 4 0.0 6 4 46 2 0.0 6 1 0 1 47 2 0.0 6 2 48 2 0.0 6 1 1 49 3 0.0 6 3 50 3 0.0 6 2 1 51 2 0.0 6 2 52 2 0.0 6 2 53 3 0.0 6 3 54 2 0.0 6 2 55 2 0.0 6 2 56 5 0.0 6 4 1 57 4 0.0 6 3 1 58 3 0.0 6 2 1 59 5 0.0 6 5 60 4 0.0 6 2 2 61 3 0.0 6 3 62 4 0.0 6 3 1 63 5 0.0 6 4 0 0 1 64 6 0.0 6 5 1 65 7 0.0 6 7 66 3 0.0 6 3 67 4 0.0 6 4 68 5 0.0 6 4 1 69 7 0.0 6 7 70 4 0.0 6 4 71 4 0.0 6 4 72 4 0.0 6 3 1 73 5 0.0 6 3 1 1 74 1 0.0 6 1 75 4 0.0 6 4 76 2 0.0 6 1 1 77 3 0.0 6 3 78 4 0.0 6 4 79 2 0.0 6 1 0 0 0 1 80 4 0.0 6 4 81 3 0.0 6 3 82 4 0.0 6 4 83 2 0.0 6 2 84 4 0.0 6 4 85 6 0.0 6 6 86 16 0.0 6 16 87 9 0.0 6 9 88 1 0.0 6 1 89 6 0.0 6 6 90 10 0.0 6 10 91 4 0.0 6 4 92 6 0.0 6 6 93 7 0.0 6 7 94 2 0.0 6 2 95 7 0.0 6 5 1 1 96 3 0.0 6 1 1 0 0 1 97 9 0.0 6 8 1 98 9 0.0 6 8 1 99 5 0.0 6 5 100 13 0.0 6 12 1 101 25 0.0 6 23 1 0 0 0 0 1 102 11 0.0 6 11 103 8 0.0 6 6 2 104 5 0.0 6 5 105 16 0.0 6 16 106 8 0.0 6 6 2 107 21 0.0 6 18 3 108 21 0.0 6 20 0 1 109 11 0.0 6 11 110 10 0.0 6 10 111 14 0.0 6 12 1 1 112 11 0.0 6 11 113 19 0.0 6 18 1 114 10 0.0 6 10 115 15 0.0 6 15 116 8 0.0 6 8 117 13 0.0 6 13 118 17 0.0 6 16 1 119 17 0.0 6 17 120 13 0.0 6 13 121 13 0.0 6 13 122 20 0.0 6 17 3 123 14 0.0 6 14 124 14 0.0 6 13 0 0 1 125 14 0.0 6 14 126 17 0.0 6 17 127 11 0.0 6 11 128 25 0.0 6 23 1 1 129 18 0.0 6 17 1 130 15 0.0 6 14 0 1 131 14 0.0 6 14 132 23 0.0 6 22 1 133 19 0.0 6 17 2 134 46 0.0 6 45 1 135 44 0.0 6 42 2 136 23 0.0 6 22 1 137 43 0.0 6 43 138 43 0.0 6 41 2 139 27 0.0 6 24 3 140 35 0.0 6 30 3 0 2 141 55 0.0 6 51 4 142 43 0.0 6 42 1 143 184 0.0 6 183 1 144 215 0.0 6 212 2 1 145 279 0.0 6 279 146 154 0.0 6 150 4 147 145 0.0 6 142 3 148 162 0.0 6 157 5 149 248 0.0 6 245 2 1 150 161 0.0 6 160 1 151 95 0.0 6 95 152 89 0.0 6 88 1 153 101 0.0 6 98 3 154 84 0.0 6 81 3 155 96 0.0 6 94 2 156 82 0.0 6 80 2 157 82 0.0 6 79 3 158 81 0.0 6 80 1 159 121 0.0 6 121 160 94 0.0 6 93 1 161 115 0.0 6 114 1 162 97 0.0 6 94 3 163 118 0.0 6 116 2 164 162 0.0 6 160 2 165 96 0.0 6 92 4 166 162 0.0 6 159 2 0 1 167 146 0.0 6 142 3 1 168 113 0.0 6 111 2 169 141 0.0 6 136 4 0 0 1 170 131 0.0 6 127 4 171 116 0.0 6 115 1 172 135 0.0 6 134 1 173 160 0.0 6 155 5 174 147 0.0 6 144 3 175 151 0.0 6 147 4 176 130 0.0 6 126 4 177 156 0.0 6 155 1 178 186 0.0 6 184 2 179 142 0.0 6 137 4 0 0 1 180 174 0.0 6 168 6 181 347 0.0 6 344 3 182 182 0.0 6 178 4 183 158 0.0 6 156 2 184 183 0.0 6 183 185 265 0.0 6 259 5 1 186 196 0.0 6 191 5 187 183 0.0 6 183 188 254 0.0 6 248 6 189 188 0.0 6 185 3 190 226 0.0 6 222 4 191 195 0.0 6 191 4 192 244 0.0 6 235 9 193 299 0.0 6 290 9 194 292 0.0 6 283 8 1 195 373 0.0 6 363 9 0 0 0 0 1 196 376 0.0 6 371 5 197 363 0.0 6 355 8 198 547 0.0 6 541 6 199 510 0.0 6 497 12 1 200 441 0.0 6 427 14 201 397 0.0 6 388 9 202 446 0.0 6 435 11 203 378 0.0 6 372 6 204 467 0.0 6 455 12 205 573 0.0 6 563 10 206 536 0.0 6 522 13 1 207 630 0.0 6 618 12 208 527 0.0 6 517 10 209 494 0.0 6 490 4 210 411 0.0 6 405 2 2 2 211 520 0.0 6 510 9 1 212 477 0.0 6 467 9 1 213 640 0.0 6 622 18 214 639 0.0 6 621 17 1 215 569 0.0 6 555 13 0 0 1 216 702 0.0 6 691 10 1 217 577 0.0 6 560 16 1 218 529 0.0 6 520 8 0 0 1 219 608 0.0 6 599 8 1 220 475 0.0 6 465 9 1 221 438 0.0 6 429 9 222 529 0.0 6 518 10 1 223 637 0.0 6 623 14 224 585 0.0 6 574 10 1 225 564 0.0 6 553 11 226 754 0.0 6 740 13 1 227 710 0.0 6 697 13 228 571 0.0 6 563 8 229 524 0.0 6 516 8 230 506 0.0 6 493 13 231 514 0.0 6 505 9 232 483 0.0 6 475 7 1 233 443 0.0 6 435 7 0 0 1 234 481 0.0 6 469 10 1 0 0 1 235 393 0.0 6 389 4 236 468 0.0 6 455 12 1 237 725 0.0 6 703 22 238 602 0.0 6 586 14 1 1 239 627 0.0 6 611 15 1 240 634 0.0 6 613 20 0 1 241 578 0.0 6 562 16 242 678 0.0 6 666 12 243 498 0.0 6 486 11 1 244 574 0.0 6 556 17 1 245 556 0.0 6 542 14 246 581 0.0 6 562 19 247 529 0.0 6 520 8 0 1 248 511 0.0 6 495 15 1 249 695 0.0 6 679 15 1 250 563 0.0 6 547 15 1 251 643 0.0 6 616 26 1 252 601 0.0 6 586 15 253 524 0.0 6 511 12 0 1 254 598 0.0 6 578 17 3 255 508 0.0 6 496 12 256 873 0.0 6 846 27 257 698 0.0 6 672 24 1 0 0 0 1 258 756 0.0 6 738 17 1 259 923 0.0 6 892 31 260 1297 0.0 6 1265 32 261 677 0.0 6 654 23 262 475 0.0 6 464 11 263 702 0.0 6 673 28 1 264 503 0.0 6 491 12 265 424 0.0 6 409 14 1 266 633 0.0 6 613 20 267 554 0.0 6 538 15 1 268 486 0.0 6 465 21 269 876 0.0 6 860 15 1 270 552 0.0 6 529 22 0 0 0 0 1 271 592 0.0 6 570 21 1 272 581 0.0 6 564 17 273 827 0.0 6 809 15 3 274 1031 0.0 6 1001 28 2 275 869 0.0 6 847 21 1 276 1710 0.0 6 1656 51 3 277 1813 0.0 6 1742 66 3 1 0 0 1 278 2524 0.0 6 2428 96 279 14053 0.0 6 13630 413 7 2 0 0 1 280 9417 0.0 6 9052 351 10 1 2 1 281 78 0.0 6 72 5 1 282 7 0.0 6 6 0 0 1 283 5 0.0 6 3 1 0 0 1 284 1 0.0 6 1 285 1 0.0 6 1 287 1 0.0 6 1 288 1 0.0 6 1 290 1 0.0 6 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 847 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 7 342.4 1 0 7 62 4 0.0 6 4 68 1 0.0 6 1 69 1 0.0 6 0 1 71 7 0.0 6 7 72 5 0.0 6 5 73 12 0.0 6 12 74 5 0.0 6 5 75 6 0.0 6 6 76 5 0.0 6 5 77 14 0.0 6 14 78 5 0.0 6 5 79 2 0.0 6 2 80 7 0.0 6 7 81 1 0.0 6 1 82 1 0.0 6 1 83 2 0.0 6 2 84 3 0.0 6 3 85 3 0.0 6 3 87 4 0.0 6 3 0 0 0 0 0 1 88 4 0.0 6 4 89 1 0.0 6 1 90 5 0.0 6 5 91 2 0.0 6 2 92 5 0.0 6 5 93 2 0.0 6 2 94 6 0.0 6 6 95 5 0.0 6 5 96 4 0.0 6 4 98 2 0.0 6 2 99 2 0.0 6 2 100 2 0.0 6 2 101 2 0.0 6 2 102 2 0.0 6 2 103 3 0.0 6 3 104 4 0.0 6 4 105 6 0.0 6 6 106 7 0.0 6 7 107 3 0.0 6 3 108 5 0.0 6 5 109 14 0.0 6 14 110 5 0.0 6 5 111 3 0.0 6 3 112 2 0.0 6 2 113 3 0.0 6 3 114 2 0.0 6 2 115 1 0.0 6 1 116 3 0.0 6 3 117 5 0.0 6 5 120 1 0.0 6 1 122 5 0.0 6 5 123 7 0.0 6 7 124 2 0.0 6 2 125 4 0.0 6 4 126 4 0.0 6 4 127 4 0.0 6 4 128 4 0.0 6 4 129 3 0.0 6 3 130 8 0.0 6 8 131 4 0.0 6 4 132 7 0.0 6 7 133 4 0.0 6 4 134 4 0.0 6 4 135 5 0.0 6 5 136 5 0.0 6 5 137 6 0.0 6 6 138 6 0.0 6 6 139 9 0.0 6 9 140 5 0.0 6 4 1 141 5 0.0 6 4 1 142 6 0.0 6 6 143 2 0.0 6 2 144 2 0.0 6 2 145 3 0.0 6 3 146 5 0.0 6 5 147 2 0.0 6 2 148 9 0.0 6 9 149 8 0.0 6 8 150 11 0.0 6 11 151 7 0.0 6 7 152 2 0.0 6 2 153 5 0.0 6 5 154 3 0.0 6 3 155 1 0.0 6 1 156 8 0.0 6 8 157 2 0.0 6 2 158 3 0.0 6 3 159 5 0.0 6 5 160 7 0.0 6 7 161 7 0.0 6 7 162 5 0.0 6 5 163 10 0.0 6 10 164 10 0.0 6 10 165 5 0.0 6 5 166 1 0.0 6 1 167 6 0.0 6 6 168 4 0.0 6 4 169 5 0.0 6 5 170 2 0.0 6 2 171 4 0.0 6 4 172 6 0.0 6 6 173 11 0.0 6 11 174 9 0.0 6 9 175 15 0.0 6 15 176 13 0.0 6 13 177 7 0.0 6 7 178 5 0.0 6 5 179 6 0.0 6 6 180 9 0.0 6 9 181 6 0.0 6 6 182 5 0.0 6 5 183 8 0.0 6 8 184 2 0.0 6 2 185 7 0.0 6 7 186 4 0.0 6 4 187 4 0.0 6 4 188 11 0.0 6 11 189 7 0.0 6 7 190 8 0.0 6 8 191 10 0.0 6 10 192 6 0.0 6 6 193 2 0.0 6 2 194 5 0.0 6 5 195 4 0.0 6 4 196 1 0.0 6 1 197 4 0.0 6 4 198 8 0.0 6 8 199 1 0.0 6 1 200 4 0.0 6 4 201 2 0.0 6 2 202 5 0.0 6 5 203 4 0.0 6 4 204 7 0.0 6 7 205 4 0.0 6 4 206 8 0.0 6 8 207 9 0.0 6 9 208 3 0.0 6 3 209 3 0.0 6 3 210 2 0.0 6 2 211 10 0.0 6 10 212 7 0.0 6 7 213 3 0.0 6 3 214 4 0.0 6 4 215 4 0.0 6 4 216 5 0.0 6 5 217 7 0.0 6 7 218 3 0.0 6 3 219 6 0.0 6 6 220 4 0.0 6 4 221 2 0.0 6 2 222 5 0.0 6 5 223 1 0.0 6 1 224 2 0.0 6 2 226 2 0.0 6 2 227 6 0.0 6 6 228 5 0.0 6 5 229 4 0.0 6 4 230 4 0.0 6 4 231 2 0.0 6 2 232 2 0.0 6 2 233 3 0.0 6 3 234 2 0.0 6 2 235 2 0.0 6 2 237 3 0.0 6 3 238 4 0.0 6 4 239 1 0.0 6 1 241 1 0.0 6 1 242 1 0.0 6 1 243 2 0.0 6 2 244 3 0.0 6 3 245 2 0.0 6 2 246 2 0.0 6 2 247 7 0.0 6 7 248 2 0.0 6 2 249 1 0.0 6 1 250 3 0.0 6 3 251 3 0.0 6 3 252 3 0.0 6 3 253 2 0.0 6 2 254 1 0.0 6 1 256 1 0.0 6 1 257 1 0.0 6 1 258 1 0.0 6 1 259 1 0.0 6 1 260 2 0.0 6 2 262 2 0.0 6 2 264 1 0.0 6 1 265 1 0.0 6 1 267 1 0.0 6 1 268 1 0.0 6 1 269 1 0.0 6 1 273 1 0.0 6 1 274 1 0.0 6 1 286 1 0.0 6 1 292 1 0.0 6 1 297 1 0.0 6 1 305 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 87020 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1369.6 0 0 2 4 1 342.4 1 1 7 1 5.4 2 0 0 1 10 3 0.1 3 2 1 11 6 0.0 3 5 0 1 12 1 0.0 3 1 13 8 0.0 3 7 1 14 8 0.0 4 7 1 16 16 0.0 4 10 6 17 15 0.0 5 14 1 18 19 0.0 5 18 1 19 14 0.0 5 14 20 85 0.0 6 82 3 21 46 0.0 6 43 3 22 34 0.0 6 32 1 1 23 34 0.0 6 32 2 24 29 0.0 6 26 3 25 30 0.0 6 29 1 26 14 0.0 6 13 1 27 19 0.0 6 17 2 28 19 0.0 6 17 2 29 19 0.0 6 18 1 30 17 0.0 6 17 31 15 0.0 6 11 4 32 11 0.0 6 8 3 33 10 0.0 6 8 2 34 14 0.0 6 12 2 35 12 0.0 6 10 2 36 14 0.0 6 13 1 37 11 0.0 6 10 1 38 7 0.0 6 6 1 39 12 0.0 6 9 2 1 40 10 0.0 6 8 1 0 0 1 41 14 0.0 6 13 1 42 10 0.0 6 7 3 43 23 0.0 6 21 2 44 26 0.0 6 23 3 45 9 0.0 6 7 2 46 19 0.0 6 18 1 47 23 0.0 6 21 2 48 21 0.0 6 20 0 1 49 22 0.0 6 20 2 50 19 0.0 6 17 2 51 9 0.0 6 9 52 31 0.0 6 26 5 53 23 0.0 6 20 3 54 21 0.0 6 18 2 1 55 24 0.0 6 21 2 1 56 16 0.0 6 13 3 57 20 0.0 6 19 1 58 24 0.0 6 21 1 1 0 1 59 10 0.0 6 8 1 1 60 15 0.0 6 13 2 61 13 0.0 6 10 3 62 24 0.0 6 19 4 1 63 25 0.0 6 21 4 64 19 0.0 6 17 2 65 17 0.0 6 16 0 0 1 66 43 0.0 6 36 5 1 1 67 180 0.0 6 167 10 2 0 0 1 68 46 0.0 6 40 4 1 0 0 0 1 69 72 0.0 6 69 2 0 0 1 70 19 0.0 6 19 71 51 0.0 6 47 4 72 28 0.0 6 27 1 73 19 0.0 6 15 3 1 74 19 0.0 6 18 0 0 0 1 75 25 0.0 6 22 2 1 76 33 0.0 6 29 4 77 28 0.0 6 24 3 1 78 32 0.0 6 28 3 1 79 38 0.0 6 34 4 80 29 0.0 6 26 3 81 33 0.0 6 26 7 82 39 0.0 6 36 3 83 70 0.0 6 65 4 1 84 45 0.0 6 42 3 85 34 0.0 6 31 3 86 31 0.0 6 26 5 87 47 0.0 6 39 7 1 88 36 0.0 6 33 2 1 89 43 0.0 6 39 2 2 90 52 0.0 6 43 9 91 49 0.0 6 44 4 1 92 44 0.0 6 43 1 93 63 0.0 6 55 5 3 94 77 0.0 6 71 5 1 95 53 0.0 6 48 5 96 59 0.0 6 55 3 0 1 97 56 0.0 6 50 4 1 1 98 63 0.0 6 58 5 99 56 0.0 6 52 4 100 69 0.0 6 67 1 1 101 66 0.0 6 62 3 1 102 106 0.0 6 100 5 0 0 0 1 103 117 0.0 6 107 8 2 104 106 0.0 6 103 3 105 84 0.0 6 76 6 2 106 75 0.0 6 73 2 107 80 0.0 6 76 3 1 108 76 0.0 6 71 4 1 109 90 0.0 6 80 9 1 110 85 0.0 6 79 6 111 97 0.0 6 90 7 112 106 0.0 6 101 3 1 0 1 113 98 0.0 6 93 5 114 130 0.0 6 125 4 1 115 107 0.0 6 96 11 116 122 0.0 6 118 3 0 1 117 90 0.0 6 86 3 1 118 120 0.0 6 110 7 3 119 128 0.0 6 123 4 0 1 120 134 0.0 6 130 4 121 120 0.0 6 109 9 1 0 1 122 112 0.0 6 104 4 2 2 123 125 0.0 6 119 6 124 166 0.0 6 160 5 0 1 125 172 0.0 6 162 9 1 126 142 0.0 6 135 7 127 180 0.0 6 173 7 128 177 0.0 6 170 6 0 0 0 0 1 129 155 0.0 6 150 5 130 182 0.0 6 170 9 2 0 1 131 265 0.0 6 260 5 132 271 0.0 6 259 12 133 295 0.0 6 284 10 0 1 134 385 0.0 6 368 17 135 396 0.0 6 384 11 0 0 0 0 1 136 280 0.0 6 268 10 2 137 339 0.0 6 328 11 138 270 0.0 6 260 7 2 0 1 139 456 0.0 6 442 12 1 0 1 140 548 0.0 6 528 18 2 141 569 0.0 6 549 17 2 0 0 1 142 583 0.0 6 569 11 1 1 1 143 503 0.0 6 484 17 1 1 144 663 0.0 6 641 19 1 0 0 2 145 567 0.0 6 555 12 146 555 0.0 6 539 11 1 0 1 1 2 147 486 0.0 6 472 13 1 148 632 0.0 6 608 21 1 0 2 149 731 0.0 6 708 22 0 0 1 150 1070 0.0 6 1039 28 0 1 1 1 151 642 0.0 6 625 13 2 1 1 152 1035 0.0 6 1010 22 2 0 1 153 828 0.0 6 809 18 1 154 744 0.0 6 723 18 0 1 2 155 717 0.0 6 701 15 0 0 0 1 156 613 0.0 6 597 15 1 157 845 0.0 6 826 18 0 0 1 158 769 0.0 6 741 27 0 0 1 159 553 0.0 6 537 15 1 160 754 0.0 6 739 14 1 161 562 0.0 6 548 13 1 162 501 0.0 6 486 15 163 613 0.0 6 592 18 2 0 0 0 1 164 676 0.0 6 658 17 1 165 635 0.0 6 615 19 0 0 0 1 166 846 0.0 6 820 23 2 0 0 1 167 612 0.0 6 595 13 0 0 2 1 1 168 989 0.0 6 956 31 1 0 0 1 169 573 0.0 6 565 8 170 605 0.0 6 584 20 1 171 984 0.0 6 956 24 1 2 1 172 646 0.0 6 632 13 0 1 173 709 0.0 6 696 10 1 0 1 0 1 174 1007 0.0 6 986 19 0 0 1 1 175 1256 0.0 6 1223 31 1 0 0 1 176 959 0.0 6 938 20 1 177 942 0.0 6 907 35 178 766 0.0 6 737 26 1 1 0 1 179 828 0.0 6 798 29 0 0 1 180 715 0.0 6 698 14 2 0 1 181 838 0.0 6 819 19 182 410 0.0 6 395 15 183 907 0.0 6 891 14 1 0 0 1 184 899 0.0 6 880 17 2 185 1050 0.0 6 1004 45 0 1 186 1544 0.0 6 1491 53 187 1370 0.0 6 1323 44 1 1 0 1 188 1374 0.0 6 1331 40 1 1 1 189 1178 0.0 6 1134 39 3 0 2 190 1609 0.0 6 1554 51 2 0 2 191 1652 0.0 6 1595 54 3 192 885 0.0 6 861 23 0 0 1 193 925 0.0 6 885 40 194 1094 0.0 6 1052 38 3 0 1 195 1504 0.0 6 1436 64 2 1 1 196 2716 0.0 6 2633 74 2 1 4 1 1 197 944 0.0 6 897 42 1 1 3 198 1758 0.0 6 1684 70 2 1 1 199 2479 0.0 6 2415 61 2 0 0 1 200 1060 0.0 6 1017 42 0 0 1 201 1406 0.0 6 1364 41 1 202 927 0.0 6 894 31 1 0 1 203 736 0.0 6 712 23 1 204 890 0.0 6 857 32 1 205 645 0.0 6 618 27 206 416 0.0 6 403 13 207 387 0.0 6 377 10 208 623 0.0 6 604 19 209 401 0.0 6 395 5 0 0 0 0 1 210 321 0.0 6 311 10 211 404 0.0 6 397 7 212 542 0.0 6 515 25 0 0 1 1 213 351 0.0 6 341 10 214 708 0.0 6 696 11 0 0 1 215 661 0.0 6 646 15 216 715 0.0 6 685 30 217 515 0.0 6 501 14 218 422 0.0 6 409 12 1 219 549 0.0 6 535 14 220 183 0.0 6 180 3 221 544 0.0 6 527 14 2 0 0 0 1 222 535 0.0 6 518 17 223 898 0.0 6 866 31 1 224 538 0.0 6 517 19 1 0 1 225 619 0.0 6 602 15 2 226 438 0.0 6 425 10 2 1 227 639 0.0 6 623 15 1 228 421 0.0 6 400 20 1 229 801 0.0 6 777 22 2 230 576 0.0 6 554 21 1 231 451 0.0 6 435 15 1 232 600 0.0 6 577 20 1 0 2 233 293 0.0 6 283 10 234 275 0.0 6 263 12 235 151 0.0 6 143 8 236 55 0.0 6 55 237 69 0.0 6 62 7 238 197 0.0 6 193 4 239 86 0.0 6 86 240 122 0.0 6 121 1 241 249 0.0 6 240 8 0 0 1 242 272 0.0 6 260 12 243 147 0.0 6 140 7 244 151 0.0 6 148 3 245 108 0.0 6 104 4 246 121 0.0 6 117 4 247 70 0.0 6 68 2 248 146 0.0 6 141 5 249 41 0.0 6 40 1 250 36 0.0 6 31 3 2 251 130 0.0 6 127 1 2 252 23 0.0 6 23 253 20 0.0 6 19 1 254 5 0.0 6 3 2 255 2 0.0 6 2 256 2 0.0 6 2 258 1 0.0 6 1 263 1 0.0 6 1 264 1 0.0 6 1 274 1 0.0 6 1 276 1 0.0 6 1 277 4 0.0 6 4 278 39 0.0 6 38 1 279 93 0.0 6 88 3 1 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 568 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 10 1 0.1 3 0 0 0 1 72 10 0.0 6 10 73 9 0.0 6 9 74 13 0.0 6 13 75 1 0.0 6 1 76 4 0.0 6 4 77 9 0.0 6 8 0 0 0 1 78 11 0.0 6 11 79 7 0.0 6 6 1 80 6 0.0 6 6 81 4 0.0 6 4 82 4 0.0 6 4 83 2 0.0 6 2 84 3 0.0 6 3 85 1 0.0 6 1 86 1 0.0 6 1 88 6 0.0 6 6 90 1 0.0 6 1 91 1 0.0 6 1 92 1 0.0 6 1 93 4 0.0 6 4 94 2 0.0 6 2 95 5 0.0 6 5 96 6 0.0 6 6 98 4 0.0 6 4 99 2 0.0 6 2 100 4 0.0 6 4 101 3 0.0 6 3 102 2 0.0 6 2 103 3 0.0 6 3 104 7 0.0 6 7 105 3 0.0 6 3 106 5 0.0 6 5 107 4 0.0 6 4 108 2 0.0 6 2 109 1 0.0 6 1 110 14 0.0 6 14 111 3 0.0 6 3 112 2 0.0 6 2 113 1 0.0 6 1 114 5 0.0 6 5 115 2 0.0 6 2 117 4 0.0 6 4 118 7 0.0 6 7 121 1 0.0 6 1 123 2 0.0 6 2 124 7 0.0 6 7 125 7 0.0 6 7 126 2 0.0 6 2 127 6 0.0 6 6 128 5 0.0 6 5 129 1 0.0 6 1 130 4 0.0 6 4 131 1 0.0 6 1 132 2 0.0 6 2 133 2 0.0 6 2 134 10 0.0 6 10 135 3 0.0 6 3 136 5 0.0 6 5 137 6 0.0 6 6 138 8 0.0 6 8 139 7 0.0 6 7 140 5 0.0 6 5 141 2 0.0 6 2 142 7 0.0 6 7 143 3 0.0 6 3 144 2 0.0 6 2 146 3 0.0 6 3 147 8 0.0 6 8 148 4 0.0 6 4 149 4 0.0 6 4 150 6 0.0 6 5 0 0 0 1 151 8 0.0 6 7 0 0 0 1 152 7 0.0 6 6 0 0 0 1 153 2 0.0 6 2 154 4 0.0 6 4 155 4 0.0 6 4 156 1 0.0 6 1 157 5 0.0 6 5 158 3 0.0 6 3 159 7 0.0 6 7 160 5 0.0 6 5 161 9 0.0 6 9 162 4 0.0 6 4 163 4 0.0 6 4 164 4 0.0 6 4 165 11 0.0 6 11 166 7 0.0 6 6 0 0 0 1 167 5 0.0 6 5 168 6 0.0 6 6 169 3 0.0 6 2 0 0 0 1 170 3 0.0 6 2 0 0 0 1 171 7 0.0 6 7 172 3 0.0 6 3 173 2 0.0 6 2 174 7 0.0 6 7 175 5 0.0 6 5 176 7 0.0 6 7 177 12 0.0 6 12 178 4 0.0 6 4 179 5 0.0 6 5 180 5 0.0 6 5 181 3 0.0 6 3 182 3 0.0 6 3 183 10 0.0 6 10 184 5 0.0 6 5 185 4 0.0 6 4 186 6 0.0 6 6 187 4 0.0 6 4 188 2 0.0 6 2 189 3 0.0 6 3 190 1 0.0 6 1 191 2 0.0 6 2 192 10 0.0 6 10 193 3 0.0 6 3 194 1 0.0 6 1 195 4 0.0 6 3 1 196 1 0.0 6 1 198 2 0.0 6 2 199 1 0.0 6 1 200 2 0.0 6 2 201 2 0.0 6 2 202 1 0.0 6 1 203 1 0.0 6 1 204 3 0.0 6 3 205 4 0.0 6 4 207 1 0.0 6 1 208 2 0.0 6 2 209 2 0.0 6 2 210 3 0.0 6 3 212 2 0.0 6 2 213 2 0.0 6 2 214 1 0.0 6 1 217 1 0.0 6 1 219 1 0.0 6 1 224 1 0.0 6 1 231 1 0.0 6 1 306 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_04_51_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_04_278_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_04_51_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_04_278_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.43 s (40 µs/read; 1.50 M reads/minute). === Summary === Total read pairs processed: 35,788 Read 1 with adapter: 35,788 (100.0%) Read 2 with adapter: 35,772 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 16 (0.0%) Pairs written (passing filters): 35,772 (100.0%) Total basepairs processed: 17,874,875 bp Read 1: 10,181,521 bp Read 2: 7,693,354 bp Total written (filtered): 2,146,152 bp (12.0%) Read 1: 1,108,719 bp Read 2: 1,037,433 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 35780 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.8% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 559.2 0 2 4 1 139.8 1 0 1 6 1 8.7 1 0 0 1 7 1 2.2 2 1 9 2 0.1 2 0 2 16 1 0.0 4 1 18 1 0.0 5 1 20 9 0.0 6 8 1 21 3 0.0 6 2 1 22 2 0.0 6 2 23 1 0.0 6 1 25 1 0.0 6 1 26 3 0.0 6 3 27 2 0.0 6 2 28 1 0.0 6 1 29 1 0.0 6 1 33 1 0.0 6 1 37 1 0.0 6 1 38 4 0.0 6 4 39 2 0.0 6 2 40 1 0.0 6 1 41 1 0.0 6 1 45 2 0.0 6 2 46 1 0.0 6 1 53 5 0.0 6 5 54 1 0.0 6 1 56 1 0.0 6 1 57 1 0.0 6 0 1 58 1 0.0 6 1 59 3 0.0 6 3 61 1 0.0 6 1 62 4 0.0 6 3 1 64 1 0.0 6 1 66 1 0.0 6 1 67 2 0.0 6 2 68 2 0.0 6 2 69 2 0.0 6 1 1 71 1 0.0 6 0 1 72 1 0.0 6 1 77 1 0.0 6 1 79 1 0.0 6 1 83 2 0.0 6 2 84 1 0.0 6 1 86 2 0.0 6 2 87 1 0.0 6 1 88 1 0.0 6 1 89 5 0.0 6 5 90 3 0.0 6 3 92 2 0.0 6 2 93 2 0.0 6 2 95 1 0.0 6 1 98 3 0.0 6 1 2 100 1 0.0 6 1 101 5 0.0 6 4 0 0 1 102 1 0.0 6 0 0 1 103 2 0.0 6 2 104 2 0.0 6 2 105 2 0.0 6 2 106 1 0.0 6 1 107 8 0.0 6 8 108 9 0.0 6 7 2 109 3 0.0 6 3 112 3 0.0 6 3 113 2 0.0 6 2 114 1 0.0 6 1 115 4 0.0 6 3 0 0 0 0 0 1 116 2 0.0 6 2 117 1 0.0 6 1 118 1 0.0 6 1 119 4 0.0 6 4 120 2 0.0 6 2 121 2 0.0 6 2 122 6 0.0 6 6 123 5 0.0 6 5 124 5 0.0 6 5 125 5 0.0 6 5 126 3 0.0 6 3 127 1 0.0 6 1 128 5 0.0 6 5 129 4 0.0 6 4 131 3 0.0 6 1 2 132 2 0.0 6 2 133 3 0.0 6 3 134 8 0.0 6 8 135 17 0.0 6 17 136 6 0.0 6 5 1 137 20 0.0 6 19 1 138 18 0.0 6 18 139 10 0.0 6 9 1 140 15 0.0 6 14 1 141 10 0.0 6 8 1 1 142 9 0.0 6 9 143 18 0.0 6 17 1 144 8 0.0 6 8 145 15 0.0 6 12 3 146 4 0.0 6 3 1 147 6 0.0 6 6 148 15 0.0 6 11 4 149 9 0.0 6 7 2 150 22 0.0 6 21 0 1 151 11 0.0 6 10 1 152 9 0.0 6 7 2 153 13 0.0 6 12 1 154 10 0.0 6 9 1 155 13 0.0 6 12 1 156 10 0.0 6 10 157 15 0.0 6 12 2 1 158 14 0.0 6 14 159 5 0.0 6 5 160 19 0.0 6 18 1 161 18 0.0 6 17 1 162 26 0.0 6 25 0 0 0 0 0 1 163 70 0.0 6 69 1 164 22 0.0 6 22 165 19 0.0 6 18 1 166 18 0.0 6 17 1 167 16 0.0 6 15 1 168 19 0.0 6 18 1 169 25 0.0 6 23 2 170 38 0.0 6 36 2 171 27 0.0 6 26 1 172 34 0.0 6 30 3 1 173 33 0.0 6 31 1 0 1 174 44 0.0 6 41 3 175 31 0.0 6 29 2 176 19 0.0 6 19 177 40 0.0 6 39 1 178 35 0.0 6 32 3 179 32 0.0 6 30 2 180 31 0.0 6 29 2 181 43 0.0 6 38 5 182 55 0.0 6 54 1 183 32 0.0 6 31 1 184 36 0.0 6 36 185 85 0.0 6 82 3 186 100 0.0 6 94 6 187 79 0.0 6 76 3 188 73 0.0 6 68 4 1 189 54 0.0 6 51 3 190 81 0.0 6 76 4 1 191 137 0.0 6 130 6 1 192 115 0.0 6 112 3 193 119 0.0 6 114 5 194 108 0.0 6 102 6 195 132 0.0 6 124 7 1 196 136 0.0 6 127 9 197 113 0.0 6 106 7 198 145 0.0 6 140 5 199 152 0.0 6 141 11 200 173 0.0 6 164 7 2 201 145 0.0 6 139 5 1 202 142 0.0 6 134 8 203 140 0.0 6 135 5 204 160 0.0 6 146 11 1 0 1 0 1 205 144 0.0 6 134 10 206 161 0.0 6 155 6 207 215 0.0 6 201 14 208 151 0.0 6 145 6 209 147 0.0 6 140 7 210 145 0.0 6 138 6 1 211 94 0.0 6 91 3 212 119 0.0 6 111 7 1 213 180 0.0 6 171 9 214 193 0.0 6 186 6 0 0 0 1 215 138 0.0 6 127 10 1 216 176 0.0 6 171 5 217 137 0.0 6 130 6 0 1 218 132 0.0 6 123 8 1 219 129 0.0 6 127 2 220 109 0.0 6 107 2 221 103 0.0 6 94 9 222 141 0.0 6 135 6 223 236 0.0 6 222 13 1 224 246 0.0 6 230 15 1 225 267 0.0 6 252 14 1 226 288 0.0 6 275 13 227 231 0.0 6 227 4 228 210 0.0 6 196 13 1 229 177 0.0 6 169 7 1 230 191 0.0 6 179 11 0 0 0 0 1 231 167 0.0 6 159 7 0 0 0 0 1 232 184 0.0 6 170 14 233 179 0.0 6 170 9 234 177 0.0 6 165 12 235 142 0.0 6 135 7 236 144 0.0 6 136 7 0 0 0 0 1 237 150 0.0 6 146 4 238 299 0.0 6 283 15 1 239 290 0.0 6 274 15 0 0 0 1 240 299 0.0 6 288 11 241 318 0.0 6 306 11 1 242 399 0.0 6 381 17 1 243 193 0.0 6 182 11 244 242 0.0 6 232 9 0 0 0 0 1 245 222 0.0 6 214 8 246 226 0.0 6 219 7 247 174 0.0 6 166 8 248 174 0.0 6 162 11 0 0 0 0 1 249 250 0.0 6 237 13 250 200 0.0 6 190 10 251 317 0.0 6 302 15 252 238 0.0 6 230 8 253 139 0.0 6 136 3 254 234 0.0 6 220 11 3 255 188 0.0 6 176 12 256 263 0.0 6 252 10 1 257 300 0.0 6 281 18 1 258 192 0.0 6 179 12 1 259 327 0.0 6 316 11 260 565 0.0 6 543 21 1 261 246 0.0 6 235 10 1 262 221 0.0 6 215 5 0 1 263 327 0.0 6 312 13 1 0 0 0 1 264 236 0.0 6 224 11 0 1 265 195 0.0 6 182 13 266 308 0.0 6 294 12 0 0 0 1 1 267 222 0.0 6 211 11 268 188 0.0 6 182 6 269 542 0.0 6 523 19 270 219 0.0 6 211 8 271 323 0.0 6 311 11 1 272 413 0.0 6 393 19 1 273 391 0.0 6 371 18 2 274 714 0.0 6 673 41 275 451 0.0 6 429 22 276 708 0.0 6 671 37 277 920 0.0 6 861 57 1 1 278 913 0.0 6 860 53 279 7984 0.0 6 7657 321 5 1 280 4855 0.0 6 4616 234 5 281 73 0.0 6 60 13 282 1 0.0 6 1 284 1 0.0 6 1 291 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 8 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 6 139.8 1 0 6 297 1 0.0 6 1 298 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 35766 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.8% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 11 2 0.0 3 2 13 3 0.0 3 2 0 1 14 2 0.0 4 1 1 15 1 0.0 4 0 1 16 2 0.0 4 2 17 5 0.0 5 4 1 19 6 0.0 5 6 20 34 0.0 6 31 3 21 25 0.0 6 23 2 22 17 0.0 6 15 2 23 12 0.0 6 11 1 24 5 0.0 6 5 25 6 0.0 6 6 26 11 0.0 6 10 1 27 6 0.0 6 5 1 28 10 0.0 6 8 2 29 3 0.0 6 3 30 3 0.0 6 2 1 31 4 0.0 6 4 32 1 0.0 6 1 33 7 0.0 6 6 1 34 5 0.0 6 5 35 5 0.0 6 5 36 3 0.0 6 3 37 6 0.0 6 6 38 4 0.0 6 4 39 5 0.0 6 5 40 5 0.0 6 4 1 41 4 0.0 6 4 42 4 0.0 6 4 43 4 0.0 6 3 1 44 13 0.0 6 13 45 4 0.0 6 3 1 46 8 0.0 6 8 47 7 0.0 6 5 2 48 8 0.0 6 6 1 0 0 1 49 10 0.0 6 8 2 50 5 0.0 6 5 51 15 0.0 6 14 1 52 12 0.0 6 11 1 53 10 0.0 6 8 2 54 2 0.0 6 2 55 21 0.0 6 15 5 0 1 56 18 0.0 6 15 3 57 16 0.0 6 14 2 58 7 0.0 6 7 59 9 0.0 6 9 60 8 0.0 6 8 61 4 0.0 6 3 1 62 4 0.0 6 1 2 1 63 7 0.0 6 5 2 64 5 0.0 6 4 1 65 4 0.0 6 4 66 5 0.0 6 2 2 0 1 67 11 0.0 6 10 0 0 0 1 68 10 0.0 6 10 69 12 0.0 6 11 0 0 0 1 70 60 0.0 6 57 2 1 71 13 0.0 6 10 3 72 44 0.0 6 43 1 73 7 0.0 6 6 1 74 16 0.0 6 15 1 75 8 0.0 6 8 76 11 0.0 6 8 3 77 14 0.0 6 13 1 78 14 0.0 6 13 1 79 11 0.0 6 9 2 80 12 0.0 6 10 2 81 12 0.0 6 12 82 13 0.0 6 11 1 1 83 26 0.0 6 25 0 1 84 24 0.0 6 23 1 85 16 0.0 6 16 86 20 0.0 6 19 1 87 27 0.0 6 25 2 88 14 0.0 6 13 0 0 0 1 89 8 0.0 6 6 2 90 22 0.0 6 17 4 1 91 18 0.0 6 16 1 1 92 9 0.0 6 9 93 19 0.0 6 18 1 94 21 0.0 6 18 2 1 95 17 0.0 6 15 2 96 36 0.0 6 31 4 1 97 24 0.0 6 23 1 98 18 0.0 6 17 1 99 27 0.0 6 23 3 1 100 36 0.0 6 31 5 101 18 0.0 6 14 4 102 40 0.0 6 37 2 0 0 1 103 38 0.0 6 34 3 0 0 1 104 60 0.0 6 54 4 0 1 1 105 43 0.0 6 41 2 106 37 0.0 6 36 0 0 1 107 55 0.0 6 52 3 108 32 0.0 6 28 4 109 30 0.0 6 29 1 110 16 0.0 6 16 111 27 0.0 6 24 3 112 28 0.0 6 28 113 28 0.0 6 27 1 114 22 0.0 6 18 4 115 35 0.0 6 34 1 116 33 0.0 6 30 3 117 20 0.0 6 18 2 118 31 0.0 6 29 1 1 119 32 0.0 6 29 3 120 58 0.0 6 55 2 1 121 38 0.0 6 33 2 0 1 2 122 45 0.0 6 45 123 32 0.0 6 29 3 124 45 0.0 6 42 3 125 36 0.0 6 35 0 1 126 38 0.0 6 36 2 127 36 0.0 6 31 3 0 2 128 59 0.0 6 51 6 1 1 129 36 0.0 6 33 3 130 45 0.0 6 44 1 131 64 0.0 6 61 3 132 70 0.0 6 67 2 0 1 133 71 0.0 6 66 4 0 1 134 88 0.0 6 85 3 135 117 0.0 6 111 3 2 1 136 85 0.0 6 78 7 137 142 0.0 6 136 5 0 1 138 105 0.0 6 100 2 1 1 1 139 126 0.0 6 119 6 0 0 1 140 88 0.0 6 82 6 141 102 0.0 6 98 4 142 140 0.0 6 129 9 0 2 143 124 0.0 6 113 10 0 0 1 144 175 0.0 6 166 8 1 145 111 0.0 6 107 3 0 0 1 146 94 0.0 6 89 5 147 67 0.0 6 64 3 148 115 0.0 6 107 7 0 1 149 122 0.0 6 116 5 1 150 224 0.0 6 215 9 151 124 0.0 6 115 9 152 284 0.0 6 265 18 0 0 1 153 268 0.0 6 262 6 154 213 0.0 6 206 6 0 1 155 324 0.0 6 315 6 1 1 1 156 216 0.0 6 205 10 1 157 297 0.0 6 286 9 0 0 2 158 269 0.0 6 261 7 0 0 1 159 116 0.0 6 114 1 0 0 1 160 300 0.0 6 288 12 161 169 0.0 6 160 7 2 162 143 0.0 6 137 6 163 231 0.0 6 220 11 164 180 0.0 6 176 4 165 227 0.0 6 214 13 166 302 0.0 6 292 9 1 167 131 0.0 6 126 5 168 314 0.0 6 301 12 0 1 169 101 0.0 6 96 5 170 114 0.0 6 106 8 171 280 0.0 6 268 12 172 115 0.0 6 105 9 1 173 178 0.0 6 169 8 0 0 1 174 265 0.0 6 253 12 175 569 0.0 6 543 25 1 176 271 0.0 6 254 17 177 388 0.0 6 375 12 0 0 1 178 231 0.0 6 218 13 179 289 0.0 6 275 11 1 0 2 180 292 0.0 6 275 15 1 1 181 448 0.0 6 429 18 0 0 1 182 182 0.0 6 170 11 1 183 411 0.0 6 395 13 1 0 2 184 77 0.0 6 73 4 185 112 0.0 6 107 5 186 481 0.0 6 464 16 0 1 187 606 0.0 6 572 31 1 0 2 188 783 0.0 6 752 30 1 189 536 0.0 6 506 27 1 0 1 1 190 983 0.0 6 946 34 3 191 789 0.0 6 763 25 0 0 1 192 578 0.0 6 559 18 0 0 1 193 356 0.0 6 345 10 1 194 610 0.0 6 581 25 4 195 676 0.0 6 645 28 0 0 3 196 2080 0.0 6 2007 71 0 0 2 197 384 0.0 6 371 12 1 198 829 0.0 6 799 29 0 1 199 2087 0.0 6 2003 77 3 0 4 200 448 0.0 6 432 16 201 802 0.0 6 776 24 2 202 472 0.0 6 461 10 1 203 319 0.0 6 309 10 204 429 0.0 6 413 15 0 0 1 205 211 0.0 6 201 9 1 206 197 0.0 6 187 9 0 1 207 126 0.0 6 120 6 208 195 0.0 6 186 9 209 207 0.0 6 200 7 210 187 0.0 6 179 7 1 211 267 0.0 6 257 9 1 212 294 0.0 6 286 7 1 213 60 0.0 6 59 1 214 156 0.0 6 149 7 215 110 0.0 6 105 4 1 216 318 0.0 6 303 14 0 1 217 624 0.0 6 598 25 0 0 1 218 300 0.0 6 291 9 219 377 0.0 6 372 5 220 236 0.0 6 222 11 2 0 1 221 506 0.0 6 495 9 1 0 1 222 340 0.0 6 331 8 0 0 1 223 440 0.0 6 423 15 1 0 1 224 230 0.0 6 220 9 0 0 1 225 368 0.0 6 352 13 1 0 2 226 258 0.0 6 249 8 1 227 224 0.0 6 216 7 0 1 228 107 0.0 6 101 6 229 264 0.0 6 255 9 230 173 0.0 6 168 5 231 204 0.0 6 191 13 232 203 0.0 6 199 3 0 0 1 233 268 0.0 6 256 12 234 103 0.0 6 101 2 235 106 0.0 6 104 2 236 36 0.0 6 35 1 237 52 0.0 6 52 238 24 0.0 6 23 1 239 25 0.0 6 25 240 63 0.0 6 61 2 241 99 0.0 6 97 1 0 0 1 242 146 0.0 6 142 4 243 47 0.0 6 46 1 244 202 0.0 6 197 5 245 54 0.0 6 52 2 246 56 0.0 6 53 2 1 247 31 0.0 6 29 2 248 41 0.0 6 40 1 249 48 0.0 6 43 4 1 250 25 0.0 6 24 1 251 30 0.0 6 29 1 252 3 0.0 6 3 253 5 0.0 6 4 1 254 26 0.0 6 24 2 255 6 0.0 6 6 258 2 0.0 6 2 277 1 0.0 6 1 280 1 0.0 6 0 1 281 9 0.0 6 8 0 1 282 11 0.0 6 11 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 6 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 14 1 0.0 4 0 0 0 0 1 45 1 0.0 6 0 0 1 91 1 0.0 6 1 92 3 0.0 6 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_05_52_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_05_279_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_05_52_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_05_279_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.68 s (22 µs/read; 2.78 M reads/minute). === Summary === Total read pairs processed: 77,601 Read 1 with adapter: 77,601 (100.0%) Read 2 with adapter: 77,547 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 54 (0.1%) Pairs written (passing filters): 77,547 (99.9%) Total basepairs processed: 39,204,946 bp Read 1: 22,448,214 bp Read 2: 16,756,732 bp Total written (filtered): 4,732,418 bp (12.1%) Read 1: 2,405,453 bp Read 2: 2,326,965 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 77570 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1212.5 0 2 4 2 303.1 1 1 1 6 1 18.9 1 0 0 1 7 2 4.7 2 0 0 1 1 11 1 0.0 3 1 19 1 0.0 5 1 20 8 0.0 6 8 21 8 0.0 6 7 1 22 5 0.0 6 5 23 4 0.0 6 4 24 1 0.0 6 1 25 9 0.0 6 8 1 26 8 0.0 6 8 27 3 0.0 6 2 1 28 2 0.0 6 2 29 2 0.0 6 2 30 3 0.0 6 3 31 1 0.0 6 0 1 33 1 0.0 6 1 34 1 0.0 6 1 35 2 0.0 6 2 36 1 0.0 6 1 37 1 0.0 6 1 38 1 0.0 6 1 39 1 0.0 6 1 40 5 0.0 6 5 41 2 0.0 6 1 1 42 2 0.0 6 1 1 43 1 0.0 6 1 44 5 0.0 6 5 45 5 0.0 6 5 47 2 0.0 6 2 48 3 0.0 6 3 49 1 0.0 6 1 50 3 0.0 6 3 52 1 0.0 6 1 53 1 0.0 6 1 54 3 0.0 6 3 55 1 0.0 6 1 56 3 0.0 6 1 2 57 5 0.0 6 5 58 1 0.0 6 1 59 2 0.0 6 2 60 1 0.0 6 1 62 4 0.0 6 3 0 1 63 3 0.0 6 3 65 3 0.0 6 3 66 1 0.0 6 1 67 1 0.0 6 1 68 2 0.0 6 2 69 1 0.0 6 1 72 2 0.0 6 2 73 2 0.0 6 1 1 74 2 0.0 6 2 78 3 0.0 6 3 79 1 0.0 6 1 81 2 0.0 6 2 82 1 0.0 6 1 84 2 0.0 6 2 85 2 0.0 6 2 86 9 0.0 6 9 87 4 0.0 6 4 88 1 0.0 6 1 89 9 0.0 6 9 90 2 0.0 6 2 91 2 0.0 6 2 92 6 0.0 6 6 93 4 0.0 6 4 94 2 0.0 6 2 95 1 0.0 6 1 96 1 0.0 6 1 97 4 0.0 6 4 98 9 0.0 6 9 99 4 0.0 6 3 1 100 7 0.0 6 6 1 101 18 0.0 6 16 2 102 5 0.0 6 4 1 103 9 0.0 6 9 104 2 0.0 6 0 2 105 6 0.0 6 5 1 106 7 0.0 6 6 0 1 107 7 0.0 6 7 108 15 0.0 6 14 1 109 4 0.0 6 4 110 1 0.0 6 0 1 111 7 0.0 6 6 0 1 112 8 0.0 6 8 113 4 0.0 6 4 114 8 0.0 6 8 115 10 0.0 6 10 116 1 0.0 6 1 117 7 0.0 6 6 1 118 2 0.0 6 1 1 119 9 0.0 6 8 1 120 1 0.0 6 1 121 12 0.0 6 10 2 122 18 0.0 6 17 1 123 11 0.0 6 9 2 124 7 0.0 6 7 125 7 0.0 6 6 1 126 9 0.0 6 9 127 6 0.0 6 6 128 17 0.0 6 14 3 129 7 0.0 6 5 2 130 9 0.0 6 8 1 131 10 0.0 6 10 132 23 0.0 6 22 1 133 10 0.0 6 9 0 1 134 25 0.0 6 25 135 34 0.0 6 31 3 136 14 0.0 6 12 2 137 42 0.0 6 42 138 14 0.0 6 13 1 139 16 0.0 6 15 1 140 21 0.0 6 19 2 141 48 0.0 6 47 1 142 27 0.0 6 26 1 143 56 0.0 6 55 1 144 34 0.0 6 31 1 1 0 0 0 1 145 37 0.0 6 37 146 41 0.0 6 40 1 147 47 0.0 6 45 1 0 1 148 26 0.0 6 24 2 149 32 0.0 6 31 1 150 34 0.0 6 33 1 151 27 0.0 6 27 152 28 0.0 6 28 153 43 0.0 6 43 154 46 0.0 6 45 1 155 42 0.0 6 40 1 1 156 26 0.0 6 26 157 41 0.0 6 39 1 1 158 28 0.0 6 27 1 159 20 0.0 6 19 1 160 13 0.0 6 12 1 161 37 0.0 6 35 2 162 37 0.0 6 35 2 163 43 0.0 6 42 1 164 43 0.0 6 42 1 165 28 0.0 6 27 1 166 32 0.0 6 32 167 46 0.0 6 46 168 34 0.0 6 33 1 169 61 0.0 6 55 6 170 72 0.0 6 70 2 171 45 0.0 6 45 172 58 0.0 6 56 2 173 81 0.0 6 81 174 83 0.0 6 80 3 175 66 0.0 6 62 4 176 62 0.0 6 62 177 68 0.0 6 66 2 178 54 0.0 6 50 4 179 58 0.0 6 55 3 180 76 0.0 6 71 4 0 1 181 74 0.0 6 74 182 86 0.0 6 86 183 62 0.0 6 59 3 184 89 0.0 6 83 5 1 185 137 0.0 6 131 6 186 163 0.0 6 160 3 187 124 0.0 6 122 2 188 174 0.0 6 169 5 189 176 0.0 6 169 7 190 175 0.0 6 173 2 191 173 0.0 6 171 2 192 172 0.0 6 165 6 1 193 207 0.0 6 199 8 194 174 0.0 6 172 2 195 229 0.0 6 223 5 0 1 196 276 0.0 6 272 4 197 232 0.0 6 224 8 198 306 0.0 6 298 8 199 298 0.0 6 286 11 1 200 294 0.0 6 285 7 1 0 0 0 1 201 237 0.0 6 234 3 202 297 0.0 6 295 2 203 295 0.0 6 289 6 204 318 0.0 6 311 7 205 285 0.0 6 278 7 206 320 0.0 6 314 6 207 360 0.0 6 355 5 208 298 0.0 6 291 5 2 209 252 0.0 6 248 4 210 235 0.0 6 231 3 1 211 180 0.0 6 179 1 212 215 0.0 6 212 3 213 308 0.0 6 299 9 214 319 0.0 6 312 7 215 245 0.0 6 238 7 216 284 0.0 6 280 4 217 297 0.0 6 291 6 218 274 0.0 6 267 6 0 0 0 1 219 255 0.0 6 251 4 220 182 0.0 6 179 3 221 234 0.0 6 228 6 222 343 0.0 6 328 14 1 223 383 0.0 6 376 7 224 273 0.0 6 267 5 0 0 0 1 225 300 0.0 6 292 7 1 226 463 0.0 6 451 12 227 362 0.0 6 350 10 2 228 305 0.0 6 300 5 229 245 0.0 6 239 6 230 255 0.0 6 251 4 231 253 0.0 6 245 8 232 250 0.0 6 247 3 233 225 0.0 6 219 6 234 273 0.0 6 267 6 235 220 0.0 6 216 4 236 223 0.0 6 220 3 237 404 0.0 6 394 9 1 238 488 0.0 6 478 10 239 505 0.0 6 493 12 240 456 0.0 6 445 10 0 0 0 0 1 241 530 0.0 6 517 13 242 539 0.0 6 528 11 243 358 0.0 6 349 8 0 1 244 396 0.0 6 387 8 1 245 477 0.0 6 463 14 246 351 0.0 6 342 9 247 271 0.0 6 260 9 1 1 248 294 0.0 6 289 5 249 417 0.0 6 408 9 250 316 0.0 6 308 8 251 510 0.0 6 500 9 0 1 252 348 0.0 6 340 8 253 246 0.0 6 241 5 254 374 0.0 6 363 10 1 255 335 0.0 6 328 7 256 441 0.0 6 436 3 2 257 381 0.0 6 372 7 2 258 305 0.0 6 294 10 1 259 494 0.0 6 481 13 260 762 0.0 6 739 23 261 347 0.0 6 344 2 1 262 306 0.0 6 300 6 263 399 0.0 6 390 8 1 264 367 0.0 6 357 10 265 300 0.0 6 292 6 1 0 0 1 266 415 0.0 6 410 5 267 273 0.0 6 268 5 268 304 0.0 6 294 10 269 1008 0.0 6 989 19 270 341 0.0 6 333 6 2 271 434 0.0 6 425 9 272 607 0.0 6 598 8 0 1 273 568 0.0 6 559 9 274 1085 0.0 6 1063 22 275 470 0.0 6 463 6 1 276 1170 0.0 6 1125 43 2 277 2038 0.0 6 2004 32 2 278 1969 0.0 6 1894 72 3 279 12408 0.0 6 12149 246 11 2 280 26477 0.0 6 25879 582 15 1 281 158 0.0 6 135 22 0 1 282 2 0.0 6 1 0 1 283 2 0.0 6 1 0 0 0 1 284 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 31 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 2 303.1 1 0 2 61 1 0.0 6 1 70 1 0.0 6 1 71 1 0.0 6 1 77 1 0.0 6 1 78 1 0.0 6 1 79 1 0.0 6 1 85 1 0.0 6 1 86 1 0.0 6 1 95 1 0.0 6 1 99 1 0.0 6 1 100 1 0.0 6 1 102 1 0.0 6 1 112 1 0.0 6 1 129 1 0.0 6 1 136 2 0.0 6 2 137 1 0.0 6 0 0 0 0 0 0 1 140 1 0.0 6 1 145 1 0.0 6 1 147 1 0.0 6 1 155 1 0.0 6 1 159 1 0.0 6 1 164 1 0.0 6 1 180 1 0.0 6 1 197 1 0.0 6 1 219 1 0.0 6 1 220 1 0.0 6 1 253 1 0.0 6 1 282 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 77519 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 2 4.7 2 2 9 1 0.3 2 1 11 5 0.0 3 5 12 2 0.0 3 2 13 16 0.0 3 16 14 10 0.0 4 10 15 1 0.0 4 1 16 9 0.0 4 9 17 17 0.0 5 16 1 18 4 0.0 5 4 19 10 0.0 5 10 20 45 0.0 6 44 1 21 44 0.0 6 42 2 22 34 0.0 6 34 23 29 0.0 6 27 1 1 24 18 0.0 6 14 4 25 21 0.0 6 21 26 5 0.0 6 5 27 14 0.0 6 14 28 5 0.0 6 4 0 1 29 9 0.0 6 9 30 12 0.0 6 12 31 5 0.0 6 5 32 9 0.0 6 9 33 9 0.0 6 8 0 1 34 10 0.0 6 10 35 5 0.0 6 4 1 36 3 0.0 6 3 37 15 0.0 6 14 0 1 38 8 0.0 6 8 39 14 0.0 6 12 2 40 16 0.0 6 15 1 41 6 0.0 6 6 42 4 0.0 6 4 43 5 0.0 6 5 44 21 0.0 6 19 2 45 11 0.0 6 9 2 46 17 0.0 6 16 1 47 22 0.0 6 19 3 48 12 0.0 6 10 1 1 49 17 0.0 6 15 2 50 25 0.0 6 23 0 2 51 10 0.0 6 10 52 26 0.0 6 26 53 10 0.0 6 10 54 22 0.0 6 18 4 55 28 0.0 6 23 4 1 56 25 0.0 6 20 5 57 9 0.0 6 9 58 11 0.0 6 10 0 0 0 0 0 1 59 25 0.0 6 21 4 60 17 0.0 6 15 2 61 7 0.0 6 7 62 9 0.0 6 5 4 63 12 0.0 6 10 2 64 15 0.0 6 14 1 65 11 0.0 6 11 66 24 0.0 6 21 3 67 33 0.0 6 30 3 68 54 0.0 6 49 5 69 142 0.0 6 134 6 0 1 1 70 35 0.0 6 32 3 71 68 0.0 6 64 4 72 50 0.0 6 46 3 0 0 1 73 21 0.0 6 18 2 1 74 23 0.0 6 22 1 75 14 0.0 6 13 0 0 0 0 1 76 26 0.0 6 26 77 29 0.0 6 28 0 0 0 1 78 34 0.0 6 32 2 79 25 0.0 6 23 2 80 32 0.0 6 30 2 81 14 0.0 6 13 1 82 33 0.0 6 31 2 83 61 0.0 6 56 5 84 70 0.0 6 64 6 85 53 0.0 6 49 4 86 33 0.0 6 32 1 87 37 0.0 6 35 2 88 23 0.0 6 22 1 89 53 0.0 6 46 6 1 90 50 0.0 6 49 1 91 45 0.0 6 44 1 92 22 0.0 6 21 1 93 67 0.0 6 65 2 94 96 0.0 6 90 4 2 95 45 0.0 6 40 4 0 1 96 53 0.0 6 49 4 97 47 0.0 6 46 0 1 98 33 0.0 6 29 3 1 99 51 0.0 6 50 0 0 1 100 59 0.0 6 50 8 1 101 30 0.0 6 27 2 1 102 87 0.0 6 80 7 103 109 0.0 6 99 7 3 104 74 0.0 6 67 7 105 95 0.0 6 91 4 106 54 0.0 6 49 4 1 107 70 0.0 6 64 5 1 108 55 0.0 6 49 6 109 62 0.0 6 59 2 1 110 53 0.0 6 51 2 111 61 0.0 6 58 2 0 0 1 112 55 0.0 6 53 2 113 48 0.0 6 43 5 114 64 0.0 6 59 5 115 77 0.0 6 73 3 0 0 0 1 116 63 0.0 6 62 1 117 60 0.0 6 57 3 118 74 0.0 6 68 6 119 106 0.0 6 98 6 0 1 1 120 128 0.0 6 126 2 121 67 0.0 6 65 2 122 63 0.0 6 61 2 123 76 0.0 6 70 6 124 109 0.0 6 102 7 125 66 0.0 6 64 2 126 57 0.0 6 55 2 127 104 0.0 6 102 2 128 66 0.0 6 65 0 1 129 47 0.0 6 46 1 130 72 0.0 6 70 1 1 131 145 0.0 6 139 6 132 115 0.0 6 112 3 133 80 0.0 6 75 5 134 152 0.0 6 143 9 135 340 0.0 6 326 10 2 0 2 136 171 0.0 6 164 7 137 246 0.0 6 240 5 0 0 0 0 1 138 135 0.0 6 128 7 139 235 0.0 6 227 6 1 0 1 140 306 0.0 6 298 8 141 251 0.0 6 240 9 2 142 399 0.0 6 389 9 1 143 240 0.0 6 230 10 144 436 0.0 6 425 11 145 248 0.0 6 238 10 146 198 0.0 6 191 7 147 139 0.0 6 133 5 1 148 204 0.0 6 192 12 149 351 0.0 6 338 12 0 0 1 150 491 0.0 6 475 14 2 151 284 0.0 6 270 12 1 0 1 152 734 0.0 6 722 11 1 153 335 0.0 6 321 11 1 0 1 1 154 462 0.0 6 445 16 1 155 398 0.0 6 388 9 0 0 0 1 156 331 0.0 6 325 6 157 462 0.0 6 446 13 2 0 1 158 455 0.0 6 438 14 2 0 1 159 229 0.0 6 219 10 160 527 0.0 6 507 19 0 1 161 309 0.0 6 302 7 162 262 0.0 6 256 5 0 0 1 163 520 0.0 6 498 22 164 352 0.0 6 339 11 0 0 2 165 565 0.0 6 542 20 3 166 599 0.0 6 577 22 167 289 0.0 6 275 14 168 638 0.0 6 626 10 1 0 1 169 209 0.0 6 200 9 170 346 0.0 6 339 6 1 171 534 0.0 6 519 14 1 172 274 0.0 6 261 11 1 1 173 501 0.0 6 492 6 3 174 731 0.0 6 708 21 0 0 2 175 1329 0.0 6 1286 40 0 1 1 0 1 176 812 0.0 6 789 22 0 1 177 798 0.0 6 779 16 2 1 178 676 0.0 6 655 18 0 0 2 1 179 717 0.0 6 697 17 3 180 703 0.0 6 684 15 3 0 0 1 181 796 0.0 6 767 25 3 1 182 322 0.0 6 303 19 183 883 0.0 6 853 29 0 1 184 153 0.0 6 146 4 2 0 0 0 1 185 372 0.0 6 356 15 1 186 1836 0.0 6 1789 44 2 1 187 1485 0.0 6 1445 38 2 188 1552 0.0 6 1508 41 1 2 189 1172 0.0 6 1126 43 2 1 190 2256 0.0 6 2196 59 1 191 1731 0.0 6 1677 51 2 0 0 1 192 1153 0.0 6 1113 38 1 0 1 193 761 0.0 6 732 28 0 1 194 1334 0.0 6 1300 31 1 1 1 195 1822 0.0 6 1746 71 1 0 4 196 3303 0.0 6 3210 86 5 0 1 0 1 197 797 0.0 6 758 38 1 198 2976 0.0 6 2890 83 0 1 2 199 3890 0.0 6 3785 101 0 1 3 200 1388 0.0 6 1354 32 0 0 2 201 1588 0.0 6 1567 21 202 886 0.0 6 856 27 2 1 203 926 0.0 6 890 33 1 1 1 204 1361 0.0 6 1324 34 2 0 1 205 404 0.0 6 395 8 0 0 1 206 396 0.0 6 382 12 2 207 279 0.0 6 268 11 208 581 0.0 6 566 14 1 209 465 0.0 6 444 20 0 1 210 505 0.0 6 481 23 0 0 1 211 689 0.0 6 669 19 1 212 605 0.0 6 594 11 213 140 0.0 6 138 2 214 411 0.0 6 397 14 215 702 0.0 6 679 21 1 0 1 216 1015 0.0 6 990 24 1 217 1014 0.0 6 983 31 218 651 0.0 6 632 17 1 1 219 679 0.0 6 661 17 0 0 1 220 459 0.0 6 443 16 221 617 0.0 6 604 10 2 1 222 270 0.0 6 262 8 223 724 0.0 6 708 15 1 224 613 0.0 6 593 19 1 225 851 0.0 6 833 17 1 226 651 0.0 6 640 10 0 0 1 227 450 0.0 6 440 10 228 295 0.0 6 283 11 0 0 1 229 577 0.0 6 561 16 230 350 0.0 6 346 4 231 370 0.0 6 357 12 1 232 449 0.0 6 435 13 1 233 624 0.0 6 609 15 234 266 0.0 6 262 4 235 91 0.0 6 88 3 236 106 0.0 6 104 2 237 91 0.0 6 88 3 238 28 0.0 6 28 239 31 0.0 6 28 3 240 164 0.0 6 159 5 241 140 0.0 6 137 3 242 238 0.0 6 231 6 0 0 1 243 343 0.0 6 335 8 244 151 0.0 6 148 3 245 60 0.0 6 60 246 65 0.0 6 60 5 247 41 0.0 6 41 248 70 0.0 6 66 4 249 44 0.0 6 43 1 250 39 0.0 6 39 251 35 0.0 6 33 2 252 14 0.0 6 14 253 48 0.0 6 48 254 7 0.0 6 7 255 10 0.0 6 10 256 1 0.0 6 1 260 2 0.0 6 1 1 261 1 0.0 6 1 280 21 0.0 6 21 281 78 0.0 6 73 5 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 28 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 2 0.0 6 2 72 1 0.0 6 1 73 1 0.0 6 1 74 1 0.0 6 1 80 1 0.0 6 1 82 1 0.0 6 1 83 1 0.0 6 1 85 1 0.0 6 1 88 2 0.0 6 2 91 1 0.0 6 1 94 1 0.0 6 1 97 1 0.0 6 1 103 1 0.0 6 1 104 2 0.0 6 2 105 1 0.0 6 1 110 2 0.0 6 2 113 1 0.0 6 1 135 1 0.0 6 1 149 1 0.0 6 1 153 1 0.0 6 1 165 2 0.0 6 2 178 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_06_53_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_06_280_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_06_53_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_06_280_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.88 s (29 µs/read; 2.04 M reads/minute). === Summary === Total read pairs processed: 63,845 Read 1 with adapter: 63,845 (100.0%) Read 2 with adapter: 63,816 (100.0%) == Read fate breakdown == Pairs that were too short: 36 (0.1%) Pairs discarded as untrimmed: 29 (0.0%) Pairs written (passing filters): 63,780 (99.9%) Total basepairs processed: 27,742,192 bp Read 1: 15,725,267 bp Read 2: 12,016,925 bp Total written (filtered): 3,908,537 bp (14.1%) Read 1: 2,033,951 bp Read 2: 1,874,586 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 62487 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 997.6 0 1 2 4 1 249.4 1 0 1 6 3 15.6 1 0 0 3 7 2 3.9 2 1 1 8 2 1.0 2 2 9 2 0.2 2 2 10 2 0.1 3 2 15 1 0.0 4 0 0 1 16 2 0.0 4 2 17 4 0.0 5 4 18 1 0.0 5 0 1 19 1 0.0 5 1 20 93 0.0 6 90 3 21 42 0.0 6 40 2 22 33 0.0 6 31 2 23 25 0.0 6 24 1 24 23 0.0 6 22 1 25 19 0.0 6 18 1 26 8 0.0 6 8 27 4 0.0 6 4 28 7 0.0 6 7 29 6 0.0 6 6 30 5 0.0 6 5 31 7 0.0 6 6 1 32 5 0.0 6 4 0 0 0 0 1 33 4 0.0 6 4 34 4 0.0 6 4 35 5 0.0 6 5 36 9 0.0 6 9 37 6 0.0 6 6 38 4 0.0 6 4 39 4 0.0 6 4 40 1 0.0 6 1 41 7 0.0 6 7 42 2 0.0 6 2 43 7 0.0 6 7 44 9 0.0 6 8 1 45 4 0.0 6 4 46 5 0.0 6 5 47 4 0.0 6 3 1 48 7 0.0 6 7 49 6 0.0 6 6 50 7 0.0 6 6 1 51 6 0.0 6 6 52 5 0.0 6 5 53 7 0.0 6 6 0 0 0 1 54 8 0.0 6 8 55 5 0.0 6 5 56 6 0.0 6 6 57 2 0.0 6 2 58 9 0.0 6 9 59 3 0.0 6 3 60 4 0.0 6 4 61 2 0.0 6 2 62 7 0.0 6 7 63 6 0.0 6 6 64 5 0.0 6 5 65 2 0.0 6 2 66 4 0.0 6 4 67 6 0.0 6 5 1 68 10 0.0 6 9 1 69 11 0.0 6 11 70 5 0.0 6 5 71 11 0.0 6 9 1 0 0 0 1 72 11 0.0 6 11 73 6 0.0 6 6 74 9 0.0 6 9 75 12 0.0 6 12 76 11 0.0 6 11 77 5 0.0 6 5 78 8 0.0 6 8 79 14 0.0 6 14 80 7 0.0 6 7 81 7 0.0 6 7 82 4 0.0 6 4 83 10 0.0 6 10 84 11 0.0 6 10 1 85 12 0.0 6 11 1 86 8 0.0 6 8 87 6 0.0 6 6 88 12 0.0 6 11 0 0 0 0 1 89 7 0.0 6 6 1 90 13 0.0 6 13 91 5 0.0 6 3 1 0 0 0 0 1 92 9 0.0 6 9 93 5 0.0 6 5 94 9 0.0 6 6 3 95 6 0.0 6 6 96 14 0.0 6 13 1 97 5 0.0 6 4 1 98 9 0.0 6 8 1 99 7 0.0 6 7 100 13 0.0 6 13 101 7 0.0 6 7 102 11 0.0 6 11 103 15 0.0 6 15 104 25 0.0 6 23 2 105 28 0.0 6 27 1 106 15 0.0 6 14 0 1 107 13 0.0 6 11 1 1 108 22 0.0 6 20 2 109 11 0.0 6 10 1 110 16 0.0 6 16 111 17 0.0 6 17 112 14 0.0 6 14 113 18 0.0 6 16 2 114 22 0.0 6 21 0 0 0 1 115 20 0.0 6 19 1 116 11 0.0 6 10 0 1 117 16 0.0 6 14 1 0 0 0 1 118 20 0.0 6 18 2 119 22 0.0 6 20 2 120 21 0.0 6 20 1 121 31 0.0 6 29 2 122 19 0.0 6 18 1 123 17 0.0 6 17 124 27 0.0 6 25 1 0 0 0 0 1 125 20 0.0 6 17 3 126 24 0.0 6 24 127 21 0.0 6 21 128 28 0.0 6 28 129 41 0.0 6 41 130 29 0.0 6 29 131 33 0.0 6 33 132 31 0.0 6 31 133 28 0.0 6 27 1 134 41 0.0 6 40 1 135 34 0.0 6 34 136 42 0.0 6 40 2 137 27 0.0 6 27 138 43 0.0 6 43 139 65 0.0 6 65 140 120 0.0 6 119 1 141 243 0.0 6 242 1 142 305 0.0 6 303 2 143 208 0.0 6 208 144 216 0.0 6 215 1 145 317 0.0 6 317 146 237 0.0 6 237 147 219 0.0 6 219 148 272 0.0 6 272 149 274 0.0 6 273 1 150 262 0.0 6 261 1 151 244 0.0 6 241 3 152 174 0.0 6 174 153 182 0.0 6 181 0 0 1 154 181 0.0 6 181 155 181 0.0 6 178 3 156 153 0.0 6 153 157 254 0.0 6 251 2 0 1 158 181 0.0 6 179 2 159 230 0.0 6 228 1 0 0 0 0 1 160 237 0.0 6 236 1 161 220 0.0 6 220 162 173 0.0 6 171 2 163 244 0.0 6 242 1 0 0 0 0 1 164 198 0.0 6 198 165 241 0.0 6 240 1 166 250 0.0 6 247 3 167 211 0.0 6 209 2 168 211 0.0 6 210 1 169 217 0.0 6 215 2 170 215 0.0 6 212 3 171 240 0.0 6 238 2 172 273 0.0 6 270 3 173 224 0.0 6 221 3 174 253 0.0 6 249 2 0 1 1 175 272 0.0 6 271 1 176 247 0.0 6 245 2 177 278 0.0 6 277 1 178 609 0.0 6 609 179 310 0.0 6 309 1 180 277 0.0 6 271 6 181 236 0.0 6 234 2 182 233 0.0 6 233 183 263 0.0 6 257 5 0 0 0 1 184 296 0.0 6 294 1 0 1 185 307 0.0 6 306 0 0 1 186 306 0.0 6 303 3 187 342 0.0 6 340 2 188 301 0.0 6 298 1 0 0 1 0 1 189 302 0.0 6 295 6 0 0 0 0 1 190 311 0.0 6 308 3 191 330 0.0 6 327 3 192 385 0.0 6 380 5 193 494 0.0 6 490 4 194 403 0.0 6 395 7 1 195 459 0.0 6 455 4 196 612 0.0 6 610 1 0 0 0 1 197 583 0.0 6 576 4 2 1 198 635 0.0 6 629 5 0 1 199 722 0.0 6 720 2 200 630 0.0 6 625 5 201 532 0.0 6 528 4 202 668 0.0 6 661 3 1 1 1 1 203 444 0.0 6 437 7 204 524 0.0 6 520 4 205 672 0.0 6 667 5 206 539 0.0 6 533 5 0 0 0 0 1 207 824 0.0 6 816 7 0 0 0 0 1 208 635 0.0 6 629 6 209 689 0.0 6 681 7 0 0 0 1 210 553 0.0 6 544 8 0 0 0 1 211 587 0.0 6 583 4 212 709 0.0 6 701 7 1 213 718 0.0 6 713 3 1 0 0 1 214 678 0.0 6 668 9 1 215 795 0.0 6 789 6 216 947 0.0 6 934 13 217 755 0.0 6 750 5 218 778 0.0 6 775 3 219 793 0.0 6 785 8 220 730 0.0 6 729 1 221 549 0.0 6 544 4 0 0 1 222 566 0.0 6 563 2 1 223 655 0.0 6 651 4 224 640 0.0 6 631 5 3 0 1 225 701 0.0 6 698 2 0 0 0 0 1 226 834 0.0 6 824 10 227 732 0.0 6 725 7 228 622 0.0 6 615 6 0 0 0 0 1 229 685 0.0 6 680 4 1 230 609 0.0 6 603 5 1 231 664 0.0 6 658 5 1 232 635 0.0 6 631 4 233 650 0.0 6 644 5 1 234 609 0.0 6 605 3 0 0 0 0 1 235 613 0.0 6 610 3 236 483 0.0 6 476 6 1 237 573 0.0 6 567 4 0 0 0 1 1 238 540 0.0 6 536 4 239 504 0.0 6 498 6 240 514 0.0 6 508 4 0 1 1 241 490 0.0 6 486 2 0 0 0 0 2 242 474 0.0 6 470 3 0 1 243 583 0.0 6 575 8 244 462 0.0 6 460 2 245 401 0.0 6 398 3 246 364 0.0 6 362 2 247 367 0.0 6 367 248 312 0.0 6 310 2 249 344 0.0 6 338 6 250 329 0.0 6 327 1 0 1 251 370 0.0 6 367 3 252 335 0.0 6 330 5 253 304 0.0 6 304 254 342 0.0 6 337 5 255 287 0.0 6 286 1 256 285 0.0 6 280 5 257 265 0.0 6 261 4 258 295 0.0 6 294 1 259 310 0.0 6 307 3 260 390 0.0 6 387 3 261 263 0.0 6 262 1 262 260 0.0 6 255 5 263 317 0.0 6 312 5 264 230 0.0 6 229 1 265 224 0.0 6 221 3 266 199 0.0 6 198 1 267 219 0.0 6 216 2 1 268 198 0.0 6 196 2 269 257 0.0 6 255 2 270 235 0.0 6 229 6 271 226 0.0 6 221 5 272 217 0.0 6 215 2 273 218 0.0 6 216 2 274 217 0.0 6 216 1 275 266 0.0 6 261 4 1 276 322 0.0 6 318 4 277 414 0.0 6 409 5 278 531 0.0 6 518 13 279 1597 0.0 6 1577 18 2 280 3073 0.0 6 3028 40 1 0 1 3 281 20 0.0 6 18 2 282 2 0.0 6 2 285 1 0.0 6 1 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1358 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 6 249.4 1 0 6 62 24 0.0 6 24 64 9 0.0 6 7 2 70 1 0.0 6 1 71 2 0.0 6 2 72 7 0.0 6 7 73 13 0.0 6 13 74 8 0.0 6 8 75 5 0.0 6 5 76 10 0.0 6 10 77 7 0.0 6 7 78 9 0.0 6 9 79 8 0.0 6 8 80 9 0.0 6 9 81 10 0.0 6 9 1 82 7 0.0 6 7 83 4 0.0 6 4 84 7 0.0 6 7 85 8 0.0 6 8 86 3 0.0 6 3 87 4 0.0 6 4 88 10 0.0 6 10 89 2 0.0 6 2 90 7 0.0 6 7 91 9 0.0 6 9 92 8 0.0 6 8 93 4 0.0 6 4 94 4 0.0 6 4 95 2 0.0 6 2 96 2 0.0 6 2 97 4 0.0 6 4 98 7 0.0 6 7 99 6 0.0 6 6 100 2 0.0 6 2 101 6 0.0 6 6 102 5 0.0 6 5 103 9 0.0 6 9 104 4 0.0 6 4 105 1 0.0 6 1 106 6 0.0 6 6 107 5 0.0 6 5 108 15 0.0 6 15 109 13 0.0 6 13 110 9 0.0 6 9 111 6 0.0 6 6 112 3 0.0 6 3 113 3 0.0 6 3 114 3 0.0 6 3 115 6 0.0 6 6 116 6 0.0 6 6 117 10 0.0 6 10 118 2 0.0 6 2 119 6 0.0 6 6 120 7 0.0 6 7 121 9 0.0 6 9 122 4 0.0 6 4 123 4 0.0 6 4 124 7 0.0 6 7 125 3 0.0 6 3 126 6 0.0 6 6 127 4 0.0 6 4 128 3 0.0 6 3 129 3 0.0 6 3 130 5 0.0 6 5 131 4 0.0 6 4 132 10 0.0 6 10 133 15 0.0 6 15 134 5 0.0 6 5 135 10 0.0 6 10 136 5 0.0 6 5 137 4 0.0 6 4 138 9 0.0 6 9 139 3 0.0 6 3 140 5 0.0 6 5 141 8 0.0 6 8 142 8 0.0 6 8 143 8 0.0 6 8 144 5 0.0 6 5 145 8 0.0 6 8 146 5 0.0 6 5 147 11 0.0 6 11 148 9 0.0 6 9 149 9 0.0 6 9 150 6 0.0 6 6 151 3 0.0 6 3 152 6 0.0 6 6 153 4 0.0 6 4 154 7 0.0 6 7 155 5 0.0 6 5 156 8 0.0 6 8 157 7 0.0 6 6 1 158 8 0.0 6 8 159 4 0.0 6 4 160 5 0.0 6 5 161 9 0.0 6 9 162 6 0.0 6 6 163 10 0.0 6 10 164 9 0.0 6 9 165 8 0.0 6 8 166 12 0.0 6 12 167 5 0.0 6 5 168 7 0.0 6 7 169 13 0.0 6 13 170 9 0.0 6 9 171 7 0.0 6 7 172 6 0.0 6 6 173 13 0.0 6 13 174 9 0.0 6 9 175 9 0.0 6 9 176 8 0.0 6 8 177 8 0.0 6 8 178 11 0.0 6 11 179 5 0.0 6 5 180 8 0.0 6 8 181 8 0.0 6 8 182 5 0.0 6 5 183 7 0.0 6 7 184 13 0.0 6 13 185 14 0.0 6 14 186 7 0.0 6 7 187 8 0.0 6 8 188 6 0.0 6 6 189 13 0.0 6 13 190 8 0.0 6 8 191 8 0.0 6 8 192 9 0.0 6 9 193 11 0.0 6 11 194 7 0.0 6 6 1 195 9 0.0 6 9 196 8 0.0 6 8 197 4 0.0 6 4 198 5 0.0 6 5 199 7 0.0 6 7 200 5 0.0 6 5 201 10 0.0 6 10 202 12 0.0 6 12 203 7 0.0 6 7 204 2 0.0 6 2 205 13 0.0 6 13 206 5 0.0 6 5 207 5 0.0 6 5 208 5 0.0 6 5 209 13 0.0 6 13 210 3 0.0 6 3 211 9 0.0 6 9 212 17 0.0 6 17 213 6 0.0 6 6 214 9 0.0 6 9 215 9 0.0 6 9 216 5 0.0 6 5 217 8 0.0 6 8 218 5 0.0 6 5 219 12 0.0 6 12 220 8 0.0 6 8 221 7 0.0 6 6 1 222 3 0.0 6 3 223 6 0.0 6 6 224 7 0.0 6 7 225 8 0.0 6 8 226 8 0.0 6 8 227 6 0.0 6 6 228 10 0.0 6 10 229 8 0.0 6 8 230 5 0.0 6 5 231 8 0.0 6 8 232 3 0.0 6 3 233 5 0.0 6 5 234 11 0.0 6 11 235 4 0.0 6 4 236 6 0.0 6 6 237 4 0.0 6 4 238 5 0.0 6 5 239 5 0.0 6 5 240 5 0.0 6 5 241 5 0.0 6 5 242 2 0.0 6 2 243 6 0.0 6 6 244 9 0.0 6 9 245 4 0.0 6 4 246 4 0.0 6 4 247 6 0.0 6 6 248 4 0.0 6 4 249 5 0.0 6 5 250 1 0.0 6 1 251 1 0.0 6 1 252 2 0.0 6 2 253 10 0.0 6 10 254 1 0.0 6 1 255 3 0.0 6 3 256 4 0.0 6 4 257 5 0.0 6 5 258 3 0.0 6 3 259 3 0.0 6 3 260 2 0.0 6 2 261 7 0.0 6 7 262 3 0.0 6 3 264 1 0.0 6 1 265 2 0.0 6 2 266 1 0.0 6 1 267 3 0.0 6 3 268 2 0.0 6 2 269 1 0.0 6 1 270 3 0.0 6 3 271 1 0.0 6 1 272 2 0.0 6 2 273 2 0.0 6 2 274 1 0.0 6 1 276 3 0.0 6 3 277 1 0.0 6 1 279 2 0.0 6 2 281 5 0.0 6 5 282 3 0.0 6 3 283 2 0.0 6 2 284 1 0.0 6 1 285 1 0.0 6 1 288 1 0.0 6 1 291 1 0.0 6 1 293 1 0.0 6 1 294 1 0.0 6 1 299 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 62922 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 15.6 1 1 7 1 3.9 2 1 8 2 1.0 2 2 9 1 0.2 2 0 1 11 5 0.0 3 5 12 3 0.0 3 3 13 8 0.0 3 8 14 8 0.0 4 6 2 16 7 0.0 4 6 1 17 17 0.0 5 15 2 18 6 0.0 5 5 1 19 44 0.0 5 42 2 20 113 0.0 6 110 3 21 50 0.0 6 50 22 35 0.0 6 34 1 23 33 0.0 6 29 4 24 28 0.0 6 27 1 25 13 0.0 6 13 26 26 0.0 6 26 27 18 0.0 6 16 2 28 14 0.0 6 13 0 1 29 12 0.0 6 11 1 30 14 0.0 6 13 1 31 13 0.0 6 12 1 32 18 0.0 6 15 3 33 20 0.0 6 17 3 34 10 0.0 6 9 1 35 10 0.0 6 10 36 13 0.0 6 11 1 1 37 13 0.0 6 12 1 38 14 0.0 6 14 39 6 0.0 6 6 40 8 0.0 6 8 41 6 0.0 6 6 42 15 0.0 6 13 2 43 15 0.0 6 15 44 9 0.0 6 9 45 17 0.0 6 14 3 46 17 0.0 6 16 0 0 1 47 12 0.0 6 11 1 48 21 0.0 6 19 1 1 49 19 0.0 6 15 4 50 18 0.0 6 15 3 51 31 0.0 6 26 5 52 23 0.0 6 22 1 53 16 0.0 6 15 1 54 27 0.0 6 27 55 23 0.0 6 20 1 1 1 56 27 0.0 6 22 2 1 1 0 1 57 20 0.0 6 17 2 1 58 23 0.0 6 21 2 59 30 0.0 6 30 60 21 0.0 6 19 1 1 61 34 0.0 6 28 4 1 1 62 26 0.0 6 26 63 33 0.0 6 33 64 18 0.0 6 18 65 27 0.0 6 25 1 0 1 66 36 0.0 6 34 2 67 32 0.0 6 30 2 68 38 0.0 6 34 2 1 0 0 1 69 36 0.0 6 34 2 70 115 0.0 6 109 3 1 0 1 1 71 46 0.0 6 44 2 72 71 0.0 6 70 1 73 50 0.0 6 45 5 74 53 0.0 6 50 1 1 0 0 0 1 75 40 0.0 6 38 2 76 49 0.0 6 46 3 77 46 0.0 6 42 2 1 1 78 49 0.0 6 48 1 79 44 0.0 6 43 1 80 39 0.0 6 36 2 0 1 81 44 0.0 6 41 1 0 1 1 82 42 0.0 6 38 4 83 63 0.0 6 60 2 1 84 59 0.0 6 56 2 1 85 67 0.0 6 64 2 0 0 0 0 1 86 77 0.0 6 74 2 0 0 1 87 36 0.0 6 34 1 1 88 47 0.0 6 45 0 0 1 1 89 47 0.0 6 42 4 1 90 44 0.0 6 43 1 91 71 0.0 6 67 4 92 55 0.0 6 50 3 0 1 1 93 63 0.0 6 59 2 1 0 0 0 1 94 69 0.0 6 65 4 95 57 0.0 6 55 0 0 0 1 0 1 96 90 0.0 6 79 9 1 0 0 1 97 72 0.0 6 69 2 0 1 98 74 0.0 6 74 99 89 0.0 6 85 3 1 100 75 0.0 6 72 2 0 0 0 0 1 101 81 0.0 6 78 2 0 0 0 1 102 91 0.0 6 84 5 1 1 103 90 0.0 6 85 3 0 1 0 1 104 109 0.0 6 106 2 1 105 88 0.0 6 87 1 106 113 0.0 6 110 3 107 159 0.0 6 155 3 0 0 0 1 108 110 0.0 6 105 4 0 1 109 117 0.0 6 115 1 0 0 0 0 1 110 100 0.0 6 95 4 0 1 111 119 0.0 6 114 4 0 0 1 112 136 0.0 6 130 6 113 127 0.0 6 124 2 0 0 0 0 1 114 147 0.0 6 140 7 115 164 0.0 6 160 4 116 152 0.0 6 151 1 117 148 0.0 6 142 5 1 118 195 0.0 6 190 5 119 182 0.0 6 178 3 1 120 192 0.0 6 186 4 0 0 1 0 1 121 206 0.0 6 199 5 1 0 1 122 217 0.0 6 213 3 0 0 1 123 164 0.0 6 155 8 0 0 1 124 189 0.0 6 182 5 1 1 125 198 0.0 6 191 5 0 2 126 189 0.0 6 180 7 1 1 127 251 0.0 6 244 6 0 0 0 0 1 128 265 0.0 6 262 3 129 265 0.0 6 253 9 0 2 0 0 1 130 290 0.0 6 281 8 0 0 0 1 131 320 0.0 6 308 8 1 1 0 1 1 132 389 0.0 6 379 8 0 2 133 414 0.0 6 406 5 2 0 0 1 134 404 0.0 6 395 6 1 1 0 1 135 425 0.0 6 413 10 1 0 0 1 136 421 0.0 6 413 6 0 1 0 1 137 503 0.0 6 499 4 138 494 0.0 6 481 11 1 0 1 139 503 0.0 6 490 12 0 0 0 0 1 140 626 0.0 6 619 5 0 0 0 2 141 662 0.0 6 650 11 1 142 680 0.0 6 673 5 1 0 1 143 701 0.0 6 686 10 1 2 1 0 1 144 779 0.0 6 770 7 1 1 145 860 0.0 6 844 14 1 0 1 146 745 0.0 6 731 11 0 1 0 1 1 147 859 0.0 6 841 15 1 1 0 0 1 148 878 0.0 6 863 13 0 0 0 1 1 149 911 0.0 6 888 18 1 0 1 1 2 150 942 0.0 6 925 12 3 1 0 1 151 1012 0.0 6 987 21 0 2 2 152 1119 0.0 6 1095 22 1 1 153 1411 0.0 6 1381 21 1 4 1 2 1 154 1088 0.0 6 1066 21 0 0 1 155 1260 0.0 6 1234 18 0 2 1 4 1 156 1296 0.0 6 1269 22 0 1 2 0 2 157 1053 0.0 6 1038 14 0 1 158 1113 0.0 6 1087 21 0 2 0 1 2 159 987 0.0 6 964 20 2 1 160 1197 0.0 6 1176 18 0 0 2 1 161 1072 0.0 6 1050 20 0 0 0 1 1 162 962 0.0 6 944 13 0 3 2 163 866 0.0 6 859 6 0 1 164 791 0.0 6 783 8 165 767 0.0 6 759 4 1 1 1 0 1 166 801 0.0 6 789 10 0 1 1 167 795 0.0 6 781 10 0 2 0 0 2 168 745 0.0 6 732 9 1 0 0 1 2 169 878 0.0 6 865 10 0 0 2 1 170 752 0.0 6 734 15 0 1 1 0 1 171 891 0.0 6 878 12 1 172 787 0.0 6 775 9 0 0 0 3 173 738 0.0 6 729 7 1 0 0 0 1 174 872 0.0 6 861 10 0 1 175 940 0.0 6 932 6 1 0 0 1 176 879 0.0 6 872 6 0 0 0 0 1 177 850 0.0 6 840 10 178 674 0.0 6 662 10 2 179 753 0.0 6 747 6 180 616 0.0 6 608 7 1 181 629 0.0 6 624 4 0 1 182 466 0.0 6 464 2 183 454 0.0 6 446 6 0 1 0 0 1 184 501 0.0 6 495 5 0 1 185 427 0.0 6 416 8 0 1 2 186 653 0.0 6 645 5 2 1 187 819 0.0 6 808 9 1 0 1 188 785 0.0 6 769 15 0 1 189 555 0.0 6 548 6 0 0 1 190 545 0.0 6 542 2 0 0 1 191 528 0.0 6 524 4 192 475 0.0 6 464 10 1 193 419 0.0 6 412 3 0 2 0 0 2 194 416 0.0 6 410 5 0 1 195 382 0.0 6 372 8 0 1 1 196 548 0.0 6 536 10 2 197 295 0.0 6 288 6 0 1 198 383 0.0 6 374 7 1 0 0 0 1 199 614 0.0 6 599 15 200 326 0.0 6 323 3 201 288 0.0 6 280 7 1 202 237 0.0 6 230 4 1 1 0 0 1 203 216 0.0 6 212 4 204 195 0.0 6 190 5 205 205 0.0 6 198 6 1 206 189 0.0 6 186 2 1 207 150 0.0 6 147 3 208 134 0.0 6 132 2 209 110 0.0 6 108 2 210 160 0.0 6 157 3 211 150 0.0 6 148 2 212 143 0.0 6 138 5 213 78 0.0 6 76 1 0 0 0 1 214 147 0.0 6 144 3 215 118 0.0 6 114 4 216 131 0.0 6 131 217 156 0.0 6 151 5 218 93 0.0 6 91 2 219 90 0.0 6 89 1 220 77 0.0 6 77 221 83 0.0 6 80 2 1 222 90 0.0 6 87 3 223 64 0.0 6 63 1 224 70 0.0 6 68 2 225 72 0.0 6 70 2 226 63 0.0 6 61 2 227 56 0.0 6 56 228 43 0.0 6 42 1 229 75 0.0 6 72 2 0 0 0 1 230 67 0.0 6 64 2 0 0 0 1 231 58 0.0 6 56 2 232 57 0.0 6 56 1 233 25 0.0 6 24 1 234 18 0.0 6 17 1 235 27 0.0 6 26 0 0 0 0 0 1 236 12 0.0 6 12 237 17 0.0 6 17 238 13 0.0 6 13 239 8 0.0 6 8 240 6 0.0 6 6 241 17 0.0 6 17 242 19 0.0 6 19 243 7 0.0 6 6 1 244 27 0.0 6 27 245 13 0.0 6 12 1 246 8 0.0 6 8 247 8 0.0 6 8 248 5 0.0 6 5 249 13 0.0 6 13 250 11 0.0 6 10 1 251 15 0.0 6 14 1 252 3 0.0 6 3 253 3 0.0 6 3 254 9 0.0 6 9 255 1 0.0 6 1 256 1 0.0 6 1 278 1 0.0 6 1 280 5 0.0 6 4 1 281 16 0.0 6 14 1 0 0 0 0 1 282 96 0.0 6 93 2 1 283 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 894 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 1 0.0 6 0 1 51 1 0.0 6 1 53 1 0.0 6 0 0 0 0 0 1 59 1 0.0 6 1 65 2 0.0 6 2 68 1 0.0 6 1 69 3 0.0 6 3 70 5 0.0 6 5 71 14 0.0 6 14 72 7 0.0 6 7 73 12 0.0 6 12 74 13 0.0 6 12 0 0 1 75 4 0.0 6 4 76 8 0.0 6 8 77 10 0.0 6 10 78 8 0.0 6 8 79 8 0.0 6 8 80 7 0.0 6 7 81 3 0.0 6 3 82 7 0.0 6 7 83 10 0.0 6 10 84 5 0.0 6 5 85 4 0.0 6 4 86 4 0.0 6 4 87 5 0.0 6 5 88 4 0.0 6 4 89 3 0.0 6 3 90 4 0.0 6 4 91 3 0.0 6 3 92 2 0.0 6 1 0 0 0 1 93 6 0.0 6 6 94 4 0.0 6 4 95 3 0.0 6 3 96 3 0.0 6 3 97 6 0.0 6 6 98 4 0.0 6 4 99 7 0.0 6 7 100 3 0.0 6 3 101 12 0.0 6 12 102 6 0.0 6 6 103 7 0.0 6 7 104 4 0.0 6 4 105 3 0.0 6 3 106 7 0.0 6 7 107 12 0.0 6 12 108 6 0.0 6 6 109 7 0.0 6 6 1 110 10 0.0 6 10 111 3 0.0 6 3 112 9 0.0 6 9 113 4 0.0 6 4 114 1 0.0 6 1 115 9 0.0 6 9 116 7 0.0 6 7 117 6 0.0 6 6 118 7 0.0 6 7 119 6 0.0 6 6 120 4 0.0 6 4 121 5 0.0 6 4 0 0 0 1 122 5 0.0 6 5 123 3 0.0 6 3 124 11 0.0 6 11 125 8 0.0 6 8 126 3 0.0 6 2 0 0 0 1 127 8 0.0 6 8 128 5 0.0 6 5 129 8 0.0 6 8 130 9 0.0 6 9 131 15 0.0 6 15 132 3 0.0 6 3 133 4 0.0 6 4 134 7 0.0 6 7 135 10 0.0 6 10 136 1 0.0 6 1 137 9 0.0 6 9 138 4 0.0 6 4 139 5 0.0 6 5 140 15 0.0 6 15 141 3 0.0 6 2 1 142 3 0.0 6 3 143 11 0.0 6 11 144 3 0.0 6 3 145 4 0.0 6 3 0 0 0 1 146 7 0.0 6 7 147 5 0.0 6 5 148 9 0.0 6 9 149 14 0.0 6 14 150 7 0.0 6 7 151 1 0.0 6 1 152 10 0.0 6 10 153 8 0.0 6 8 154 7 0.0 6 7 155 11 0.0 6 10 0 0 0 1 156 3 0.0 6 2 1 157 12 0.0 6 12 158 7 0.0 6 7 159 4 0.0 6 4 160 9 0.0 6 9 161 17 0.0 6 17 162 3 0.0 6 3 163 12 0.0 6 12 164 13 0.0 6 13 165 5 0.0 6 5 166 5 0.0 6 5 167 7 0.0 6 7 168 3 0.0 6 3 169 5 0.0 6 5 170 8 0.0 6 7 1 171 15 0.0 6 15 172 6 0.0 6 6 173 4 0.0 6 4 174 5 0.0 6 5 175 9 0.0 6 9 176 11 0.0 6 11 177 8 0.0 6 8 178 3 0.0 6 3 179 6 0.0 6 6 180 7 0.0 6 7 181 8 0.0 6 8 182 8 0.0 6 8 183 5 0.0 6 5 184 6 0.0 6 6 185 5 0.0 6 5 186 6 0.0 6 6 187 3 0.0 6 3 188 2 0.0 6 2 189 5 0.0 6 5 190 5 0.0 6 5 191 5 0.0 6 5 192 3 0.0 6 3 193 2 0.0 6 2 194 4 0.0 6 4 195 3 0.0 6 3 196 2 0.0 6 2 197 5 0.0 6 5 198 7 0.0 6 7 199 4 0.0 6 4 200 1 0.0 6 1 201 1 0.0 6 1 202 5 0.0 6 5 203 2 0.0 6 2 204 4 0.0 6 4 205 2 0.0 6 2 207 3 0.0 6 3 208 1 0.0 6 1 209 2 0.0 6 2 210 2 0.0 6 2 212 2 0.0 6 2 213 3 0.0 6 3 214 2 0.0 6 2 215 4 0.0 6 4 216 2 0.0 6 2 217 4 0.0 6 4 219 1 0.0 6 1 220 1 0.0 6 1 225 2 0.0 6 2 226 1 0.0 6 1 227 1 0.0 6 1 228 2 0.0 6 2 231 1 0.0 6 1 233 1 0.0 6 1 239 1 0.0 6 0 1 260 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_15_54_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_15_281_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_15_54_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_15_281_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.49 s (22 µs/read; 2.79 M reads/minute). === Summary === Total read pairs processed: 69,470 Read 1 with adapter: 69,470 (100.0%) Read 2 with adapter: 69,429 (99.9%) == Read fate breakdown == Pairs that were too short: 34 (0.0%) Pairs discarded as untrimmed: 41 (0.1%) Pairs written (passing filters): 69,395 (99.9%) Total basepairs processed: 29,094,107 bp Read 1: 15,944,583 bp Read 2: 13,149,524 bp Total written (filtered): 4,582,431 bp (15.8%) Read 1: 2,297,310 bp Read 2: 2,285,121 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 66939 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1085.5 0 0 2 6 4 17.0 1 1 0 3 7 2 4.2 2 0 0 2 8 3 1.1 2 3 9 3 0.3 2 3 10 3 0.1 3 3 11 1 0.0 3 1 13 1 0.0 3 1 15 3 0.0 4 2 1 16 4 0.0 4 3 1 17 4 0.0 5 4 20 147 0.0 6 142 5 21 79 0.0 6 78 1 22 55 0.0 6 49 6 23 38 0.0 6 35 2 0 0 0 1 24 21 0.0 6 19 1 1 25 23 0.0 6 23 26 22 0.0 6 21 1 27 15 0.0 6 13 1 0 0 0 1 28 9 0.0 6 9 29 10 0.0 6 10 30 5 0.0 6 5 31 8 0.0 6 7 0 1 32 8 0.0 6 8 33 7 0.0 6 7 34 6 0.0 6 6 35 4 0.0 6 4 36 9 0.0 6 8 1 37 5 0.0 6 5 38 9 0.0 6 9 39 7 0.0 6 7 40 6 0.0 6 6 41 11 0.0 6 10 1 42 6 0.0 6 6 43 9 0.0 6 8 0 0 0 1 44 7 0.0 6 7 45 4 0.0 6 4 46 5 0.0 6 3 2 47 3 0.0 6 3 48 4 0.0 6 4 49 9 0.0 6 7 2 50 7 0.0 6 6 0 0 1 51 10 0.0 6 9 0 1 52 8 0.0 6 7 1 53 9 0.0 6 7 1 1 54 6 0.0 6 5 0 0 0 1 55 7 0.0 6 7 56 5 0.0 6 4 0 0 1 57 6 0.0 6 5 1 58 5 0.0 6 5 59 1 0.0 6 1 60 5 0.0 6 5 61 5 0.0 6 5 62 5 0.0 6 5 63 4 0.0 6 3 0 0 1 64 4 0.0 6 4 65 9 0.0 6 9 66 5 0.0 6 5 67 9 0.0 6 9 68 10 0.0 6 10 69 3 0.0 6 3 70 4 0.0 6 4 71 7 0.0 6 6 1 72 10 0.0 6 8 2 73 5 0.0 6 5 74 7 0.0 6 7 75 12 0.0 6 11 1 76 6 0.0 6 6 77 7 0.0 6 7 78 7 0.0 6 6 1 79 4 0.0 6 4 80 13 0.0 6 11 1 1 81 7 0.0 6 7 82 8 0.0 6 6 2 83 11 0.0 6 11 84 10 0.0 6 10 85 9 0.0 6 9 86 7 0.0 6 6 1 87 8 0.0 6 8 88 12 0.0 6 11 1 89 9 0.0 6 9 90 9 0.0 6 9 91 13 0.0 6 13 92 4 0.0 6 4 93 5 0.0 6 5 94 9 0.0 6 8 1 95 8 0.0 6 8 96 9 0.0 6 9 97 9 0.0 6 9 98 8 0.0 6 8 99 12 0.0 6 12 100 9 0.0 6 9 101 19 0.0 6 19 102 11 0.0 6 11 103 19 0.0 6 17 2 104 16 0.0 6 15 1 105 26 0.0 6 25 0 0 0 0 1 106 17 0.0 6 16 1 107 14 0.0 6 12 2 108 16 0.0 6 14 2 109 15 0.0 6 15 110 26 0.0 6 26 111 23 0.0 6 23 112 16 0.0 6 15 0 0 1 113 24 0.0 6 24 114 13 0.0 6 12 1 115 16 0.0 6 15 1 116 24 0.0 6 24 117 26 0.0 6 25 1 118 21 0.0 6 20 1 119 25 0.0 6 24 0 0 0 1 120 18 0.0 6 17 0 0 0 0 1 121 17 0.0 6 16 0 0 0 0 1 122 19 0.0 6 19 123 17 0.0 6 17 124 20 0.0 6 20 125 25 0.0 6 24 1 126 28 0.0 6 28 127 28 0.0 6 28 128 22 0.0 6 22 129 24 0.0 6 23 1 130 25 0.0 6 24 0 1 131 36 0.0 6 35 1 132 36 0.0 6 36 133 32 0.0 6 30 2 134 38 0.0 6 38 135 37 0.0 6 37 136 36 0.0 6 36 137 42 0.0 6 42 138 51 0.0 6 51 139 44 0.0 6 43 1 140 58 0.0 6 58 141 69 0.0 6 69 142 76 0.0 6 76 143 367 0.0 6 367 144 567 0.0 6 567 145 535 0.0 6 532 3 146 489 0.0 6 489 147 392 0.0 6 391 0 0 0 0 1 148 649 0.0 6 648 0 1 149 732 0.0 6 731 1 150 1223 0.0 6 1221 2 151 501 0.0 6 498 2 1 152 437 0.0 6 436 1 153 1023 0.0 6 1022 1 154 656 0.0 6 656 155 274 0.0 6 273 1 156 268 0.0 6 266 2 157 235 0.0 6 234 1 158 208 0.0 6 206 2 159 337 0.0 6 336 0 0 0 0 1 160 387 0.0 6 387 161 393 0.0 6 392 1 162 386 0.0 6 384 2 163 612 0.0 6 612 164 429 0.0 6 428 1 165 313 0.0 6 310 2 1 166 377 0.0 6 373 4 167 250 0.0 6 248 2 168 281 0.0 6 281 169 464 0.0 6 463 1 170 580 0.0 6 579 1 171 276 0.0 6 276 172 289 0.0 6 288 1 173 552 0.0 6 550 2 174 725 0.0 6 722 3 175 575 0.0 6 575 176 358 0.0 6 356 2 177 478 0.0 6 476 2 178 411 0.0 6 411 179 355 0.0 6 354 1 180 785 0.0 6 785 181 942 0.0 6 941 1 182 373 0.0 6 371 2 183 401 0.0 6 399 2 184 749 0.0 6 748 1 185 559 0.0 6 559 186 448 0.0 6 446 2 187 778 0.0 6 775 3 188 741 0.0 6 739 2 189 447 0.0 6 446 1 190 526 0.0 6 523 3 191 416 0.0 6 416 192 363 0.0 6 360 2 0 0 0 0 1 193 515 0.0 6 511 4 194 372 0.0 6 370 1 0 0 0 1 195 494 0.0 6 488 6 196 946 0.0 6 942 3 0 1 197 775 0.0 6 769 6 198 954 0.0 6 950 3 1 199 1240 0.0 6 1237 1 0 0 0 0 2 200 688 0.0 6 684 4 201 521 0.0 6 518 3 202 781 0.0 6 777 3 0 1 203 710 0.0 6 705 4 1 204 663 0.0 6 658 5 205 846 0.0 6 841 5 206 741 0.0 6 736 5 207 779 0.0 6 775 4 208 832 0.0 6 831 1 209 958 0.0 6 957 1 210 728 0.0 6 721 7 211 597 0.0 6 595 1 1 212 868 0.0 6 863 4 0 0 1 213 718 0.0 6 715 3 214 803 0.0 6 799 4 215 755 0.0 6 748 7 216 944 0.0 6 938 6 217 788 0.0 6 783 5 218 837 0.0 6 833 3 0 0 1 219 755 0.0 6 750 5 220 723 0.0 6 721 1 1 221 528 0.0 6 525 3 222 453 0.0 6 449 3 1 223 604 0.0 6 603 1 224 522 0.0 6 520 2 225 541 0.0 6 540 0 1 226 692 0.0 6 688 4 227 628 0.0 6 626 2 228 519 0.0 6 511 7 0 0 1 229 548 0.0 6 544 4 230 495 0.0 6 495 231 459 0.0 6 457 1 0 0 0 0 1 232 424 0.0 6 422 2 233 413 0.0 6 412 1 234 436 0.0 6 435 1 235 411 0.0 6 408 2 0 0 1 236 414 0.0 6 411 3 237 462 0.0 6 462 238 430 0.0 6 429 1 239 310 0.0 6 307 3 240 398 0.0 6 396 2 241 444 0.0 6 438 5 0 0 0 0 1 242 374 0.0 6 373 1 243 342 0.0 6 341 1 244 328 0.0 6 324 3 1 245 219 0.0 6 217 2 246 293 0.0 6 289 2 0 2 247 244 0.0 6 242 0 0 0 0 1 1 248 241 0.0 6 239 2 249 233 0.0 6 229 3 0 0 0 1 250 234 0.0 6 232 1 0 1 251 200 0.0 6 198 2 252 180 0.0 6 178 2 253 184 0.0 6 183 1 254 180 0.0 6 178 2 255 170 0.0 6 168 0 0 0 0 0 2 256 184 0.0 6 182 2 257 147 0.0 6 147 258 143 0.0 6 143 259 162 0.0 6 162 260 194 0.0 6 192 2 261 164 0.0 6 162 2 262 117 0.0 6 116 1 263 104 0.0 6 104 264 111 0.0 6 110 1 265 104 0.0 6 103 1 266 110 0.0 6 109 1 267 119 0.0 6 119 268 92 0.0 6 91 1 269 122 0.0 6 120 2 270 125 0.0 6 125 271 92 0.0 6 92 272 91 0.0 6 91 273 97 0.0 6 96 1 274 107 0.0 6 105 2 275 137 0.0 6 136 1 276 142 0.0 6 139 3 277 183 0.0 6 182 1 278 179 0.0 6 177 2 279 736 0.0 6 733 2 0 0 0 0 1 280 1244 0.0 6 1231 12 0 0 0 0 1 281 8 0.0 6 8 282 1 0.0 6 1 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2531 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 6 271.4 1 0 6 60 1 0.0 6 0 1 61 7 0.0 6 1 6 62 415 0.0 6 402 9 4 63 1 0.0 6 1 70 2 0.0 6 2 71 14 0.0 6 14 72 16 0.0 6 16 73 12 0.0 6 12 74 16 0.0 6 16 75 18 0.0 6 18 76 17 0.0 6 17 77 37 0.0 6 37 78 60 0.0 6 60 79 25 0.0 6 25 80 16 0.0 6 16 81 46 0.0 6 46 82 12 0.0 6 12 83 6 0.0 6 6 84 4 0.0 6 4 85 8 0.0 6 8 86 9 0.0 6 9 87 19 0.0 6 19 88 14 0.0 6 14 89 13 0.0 6 13 90 15 0.0 6 15 91 15 0.0 6 15 92 17 0.0 6 17 93 9 0.0 6 9 94 12 0.0 6 12 95 2 0.0 6 2 96 8 0.0 6 8 97 15 0.0 6 15 98 13 0.0 6 13 99 6 0.0 6 6 100 6 0.0 6 6 101 12 0.0 6 12 102 20 0.0 6 20 103 15 0.0 6 14 1 104 6 0.0 6 6 105 14 0.0 6 14 106 7 0.0 6 7 107 23 0.0 6 23 108 32 0.0 6 32 109 40 0.0 6 40 110 11 0.0 6 11 111 15 0.0 6 15 112 19 0.0 6 18 1 113 15 0.0 6 14 0 0 0 0 0 1 114 13 0.0 6 13 115 19 0.0 6 19 116 18 0.0 6 18 117 15 0.0 6 15 118 7 0.0 6 7 119 15 0.0 6 15 120 11 0.0 6 11 121 12 0.0 6 12 122 1 0.0 6 1 123 7 0.0 6 7 124 21 0.0 6 21 125 13 0.0 6 13 126 22 0.0 6 22 127 29 0.0 6 29 128 13 0.0 6 13 129 15 0.0 6 15 130 20 0.0 6 20 131 18 0.0 6 18 132 11 0.0 6 11 133 13 0.0 6 13 134 13 0.0 6 13 135 10 0.0 6 10 136 27 0.0 6 26 1 137 21 0.0 6 21 138 25 0.0 6 25 139 16 0.0 6 16 140 30 0.0 6 30 141 17 0.0 6 17 142 22 0.0 6 22 143 9 0.0 6 9 144 8 0.0 6 8 145 27 0.0 6 27 146 21 0.0 6 21 147 12 0.0 6 12 148 14 0.0 6 14 149 14 0.0 6 14 150 16 0.0 6 16 151 26 0.0 6 26 152 10 0.0 6 10 153 10 0.0 6 10 154 23 0.0 6 23 155 10 0.0 6 10 156 11 0.0 6 11 157 24 0.0 6 24 158 10 0.0 6 10 159 11 0.0 6 11 160 5 0.0 6 5 161 8 0.0 6 8 162 7 0.0 6 7 163 10 0.0 6 10 164 6 0.0 6 6 165 15 0.0 6 15 166 12 0.0 6 12 167 8 0.0 6 8 168 7 0.0 6 7 169 26 0.0 6 26 170 18 0.0 6 18 171 18 0.0 6 18 172 12 0.0 6 12 173 6 0.0 6 6 174 10 0.0 6 10 175 10 0.0 6 10 176 11 0.0 6 11 177 9 0.0 6 9 178 5 0.0 6 4 1 179 10 0.0 6 10 180 6 0.0 6 6 181 9 0.0 6 9 182 7 0.0 6 7 183 4 0.0 6 4 184 8 0.0 6 8 185 13 0.0 6 13 186 17 0.0 6 17 187 15 0.0 6 15 188 8 0.0 6 8 189 13 0.0 6 13 190 11 0.0 6 11 191 10 0.0 6 10 192 3 0.0 6 3 193 5 0.0 6 5 194 2 0.0 6 2 195 6 0.0 6 6 196 11 0.0 6 11 197 12 0.0 6 12 198 10 0.0 6 10 199 13 0.0 6 13 200 7 0.0 6 7 201 5 0.0 6 5 202 12 0.0 6 12 203 10 0.0 6 10 204 1 0.0 6 1 205 8 0.0 6 8 206 3 0.0 6 3 207 8 0.0 6 8 208 5 0.0 6 5 209 2 0.0 6 2 210 1 0.0 6 1 211 6 0.0 6 6 212 1 0.0 6 1 213 9 0.0 6 9 214 12 0.0 6 12 215 7 0.0 6 7 216 4 0.0 6 4 217 2 0.0 6 2 218 2 0.0 6 2 220 2 0.0 6 2 221 6 0.0 6 6 222 2 0.0 6 2 223 1 0.0 6 1 224 3 0.0 6 3 225 1 0.0 6 1 226 2 0.0 6 2 227 5 0.0 6 5 228 4 0.0 6 4 229 3 0.0 6 3 230 5 0.0 6 5 231 3 0.0 6 3 232 3 0.0 6 3 233 4 0.0 6 4 234 2 0.0 6 2 235 7 0.0 6 7 236 1 0.0 6 1 237 1 0.0 6 1 238 2 0.0 6 2 239 3 0.0 6 3 240 8 0.0 6 8 241 1 0.0 6 1 242 3 0.0 6 3 243 1 0.0 6 1 244 5 0.0 6 5 245 3 0.0 6 3 246 3 0.0 6 3 247 4 0.0 6 4 248 9 0.0 6 9 249 4 0.0 6 4 250 1 0.0 6 1 251 3 0.0 6 3 252 3 0.0 6 3 253 3 0.0 6 3 254 3 0.0 6 3 255 1 0.0 6 1 256 3 0.0 6 2 1 257 2 0.0 6 2 258 2 0.0 6 2 259 1 0.0 6 1 260 2 0.0 6 2 261 1 0.0 6 1 262 1 0.0 6 1 264 1 0.0 6 1 267 1 0.0 6 1 268 1 0.0 6 1 269 3 0.0 6 3 270 1 0.0 6 1 271 1 0.0 6 1 272 2 0.0 6 2 273 1 0.0 6 1 274 1 0.0 6 1 275 1 0.0 6 1 276 2 0.0 6 2 277 5 0.0 6 5 278 1 0.0 6 1 279 1 0.0 6 1 283 1 0.0 6 1 284 2 0.0 6 2 285 1 0.0 6 1 287 1 0.0 6 1 288 2 0.0 6 2 293 1 0.0 6 1 300 1 0.0 6 1 305 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 67620 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 9 2 0.3 2 2 10 5 0.1 3 4 1 12 1 0.0 3 0 1 13 12 0.0 3 11 1 14 9 0.0 4 9 16 8 0.0 4 7 1 17 19 0.0 5 16 3 18 14 0.0 5 11 2 1 19 19 0.0 5 19 20 114 0.0 6 109 4 1 21 53 0.0 6 51 2 22 30 0.0 6 28 1 0 1 23 31 0.0 6 31 24 18 0.0 6 13 5 25 19 0.0 6 18 1 26 25 0.0 6 25 27 15 0.0 6 15 28 10 0.0 6 10 29 17 0.0 6 17 30 23 0.0 6 19 4 31 12 0.0 6 12 32 20 0.0 6 18 2 33 14 0.0 6 13 1 34 15 0.0 6 13 2 35 12 0.0 6 12 36 13 0.0 6 12 1 37 11 0.0 6 10 1 38 11 0.0 6 10 1 39 10 0.0 6 8 1 0 1 40 9 0.0 6 8 0 1 41 11 0.0 6 10 0 1 42 13 0.0 6 13 43 9 0.0 6 9 44 13 0.0 6 11 2 45 8 0.0 6 7 1 46 9 0.0 6 8 1 47 12 0.0 6 9 3 48 13 0.0 6 12 1 49 19 0.0 6 16 3 50 11 0.0 6 8 3 51 20 0.0 6 19 0 0 0 1 52 13 0.0 6 13 53 27 0.0 6 25 2 54 21 0.0 6 20 1 55 13 0.0 6 13 56 9 0.0 6 8 0 1 57 14 0.0 6 11 2 1 58 29 0.0 6 24 5 59 22 0.0 6 19 3 60 20 0.0 6 19 0 1 61 11 0.0 6 10 1 62 20 0.0 6 19 1 63 23 0.0 6 19 4 64 19 0.0 6 15 3 1 65 28 0.0 6 25 3 66 39 0.0 6 38 0 1 67 128 0.0 6 125 3 68 50 0.0 6 47 2 0 1 69 61 0.0 6 57 4 70 39 0.0 6 39 71 46 0.0 6 44 2 72 27 0.0 6 25 2 73 34 0.0 6 31 2 0 0 0 1 74 30 0.0 6 26 3 1 75 20 0.0 6 17 2 0 1 76 35 0.0 6 35 77 27 0.0 6 27 78 35 0.0 6 33 2 79 40 0.0 6 37 3 80 46 0.0 6 43 3 81 40 0.0 6 38 2 82 60 0.0 6 58 2 83 74 0.0 6 72 1 0 0 1 84 39 0.0 6 38 1 85 33 0.0 6 29 2 2 86 21 0.0 6 20 1 87 31 0.0 6 29 1 1 88 46 0.0 6 44 2 89 36 0.0 6 36 90 58 0.0 6 57 1 91 58 0.0 6 52 5 0 0 1 92 57 0.0 6 54 2 0 0 0 1 93 45 0.0 6 44 1 94 63 0.0 6 61 1 1 95 66 0.0 6 63 2 0 1 96 96 0.0 6 90 5 0 0 0 0 1 97 62 0.0 6 58 4 98 61 0.0 6 60 1 99 59 0.0 6 57 2 100 65 0.0 6 61 3 0 1 101 55 0.0 6 54 1 102 69 0.0 6 65 3 1 103 85 0.0 6 82 2 1 104 145 0.0 6 139 4 0 1 0 0 1 105 92 0.0 6 89 3 106 65 0.0 6 61 4 107 76 0.0 6 73 3 108 87 0.0 6 83 4 109 87 0.0 6 86 0 1 110 107 0.0 6 102 5 111 105 0.0 6 102 2 0 0 0 0 1 112 124 0.0 6 122 2 113 138 0.0 6 134 4 114 103 0.0 6 101 2 115 140 0.0 6 137 2 1 116 131 0.0 6 127 3 0 0 0 0 1 117 136 0.0 6 134 2 118 141 0.0 6 138 2 0 0 0 1 119 132 0.0 6 124 5 1 1 0 0 1 120 126 0.0 6 120 6 121 139 0.0 6 132 6 0 0 0 0 1 122 157 0.0 6 153 3 1 123 175 0.0 6 170 5 124 223 0.0 6 216 6 0 1 125 255 0.0 6 253 2 126 222 0.0 6 219 2 0 0 1 127 246 0.0 6 244 1 0 0 0 1 128 267 0.0 6 261 4 2 129 321 0.0 6 311 7 1 1 0 1 130 576 0.0 6 561 13 2 131 382 0.0 6 365 14 0 0 1 2 132 341 0.0 6 337 3 0 1 133 368 0.0 6 359 8 0 0 1 134 442 0.0 6 437 4 0 0 0 0 1 135 391 0.0 6 387 2 1 0 1 136 416 0.0 6 408 8 137 408 0.0 6 405 3 138 476 0.0 6 463 10 1 0 2 139 711 0.0 6 702 7 1 0 0 0 1 140 882 0.0 6 878 4 141 949 0.0 6 946 3 142 948 0.0 6 938 9 1 143 878 0.0 6 869 7 0 1 0 1 144 1134 0.0 6 1121 9 2 1 1 145 1229 0.0 6 1221 7 0 0 0 1 146 1625 0.0 6 1615 10 147 1133 0.0 6 1122 9 1 1 148 1213 0.0 6 1199 10 2 1 0 0 1 149 1583 0.0 6 1568 10 1 1 1 1 1 150 1719 0.0 6 1701 15 2 0 1 151 1041 0.0 6 1032 8 0 0 0 1 152 1323 0.0 6 1303 16 2 0 0 2 153 1256 0.0 6 1243 11 0 0 1 1 154 1141 0.0 6 1128 9 0 0 1 1 2 155 1205 0.0 6 1184 17 0 0 2 1 1 156 1081 0.0 6 1069 11 0 0 0 1 157 1194 0.0 6 1183 8 0 2 0 1 158 1170 0.0 6 1156 12 1 0 0 0 1 159 1152 0.0 6 1144 7 0 1 160 1083 0.0 6 1077 2 2 1 1 161 971 0.0 6 960 7 1 1 2 162 892 0.0 6 887 3 0 2 163 971 0.0 6 963 8 164 934 0.0 6 923 9 0 0 0 0 2 165 903 0.0 6 899 4 166 1206 0.0 6 1197 8 1 167 899 0.0 6 891 6 1 0 1 168 1153 0.0 6 1147 6 169 1018 0.0 6 1013 3 0 0 2 170 1084 0.0 6 1072 11 0 0 0 1 171 1059 0.0 6 1050 7 1 0 0 1 172 1059 0.0 6 1053 6 173 964 0.0 6 953 11 174 991 0.0 6 983 8 175 956 0.0 6 949 7 176 1158 0.0 6 1152 4 1 0 1 177 831 0.0 6 827 1 0 0 0 2 1 178 848 0.0 6 842 4 0 1 0 1 179 689 0.0 6 686 2 1 180 608 0.0 6 603 4 1 181 564 0.0 6 556 6 1 0 0 0 1 182 567 0.0 6 565 2 183 869 0.0 6 865 4 184 989 0.0 6 986 3 185 868 0.0 6 866 1 1 186 611 0.0 6 607 3 1 187 598 0.0 6 595 3 188 509 0.0 6 506 3 189 522 0.0 6 521 1 190 472 0.0 6 470 2 191 521 0.0 6 519 2 192 397 0.0 6 394 3 193 362 0.0 6 359 3 194 265 0.0 6 263 2 195 257 0.0 6 255 1 0 0 1 196 416 0.0 6 415 1 197 283 0.0 6 283 198 261 0.0 6 259 1 0 1 199 217 0.0 6 215 2 200 191 0.0 6 191 201 191 0.0 6 190 1 202 195 0.0 6 194 1 203 179 0.0 6 179 204 155 0.0 6 152 2 1 205 125 0.0 6 124 0 0 0 1 206 116 0.0 6 114 2 207 114 0.0 6 114 208 100 0.0 6 100 209 80 0.0 6 78 1 0 0 0 0 1 210 71 0.0 6 70 1 211 86 0.0 6 86 212 104 0.0 6 104 213 90 0.0 6 90 214 97 0.0 6 97 215 66 0.0 6 65 1 216 70 0.0 6 70 217 42 0.0 6 42 218 61 0.0 6 59 2 219 43 0.0 6 43 220 34 0.0 6 33 0 0 0 0 0 1 221 47 0.0 6 47 222 22 0.0 6 22 223 26 0.0 6 26 224 25 0.0 6 25 225 20 0.0 6 20 226 26 0.0 6 26 227 22 0.0 6 22 228 10 0.0 6 10 229 8 0.0 6 8 230 10 0.0 6 10 231 10 0.0 6 10 232 8 0.0 6 8 233 10 0.0 6 10 234 3 0.0 6 3 235 6 0.0 6 6 237 5 0.0 6 5 238 5 0.0 6 4 1 239 2 0.0 6 2 240 4 0.0 6 4 241 2 0.0 6 2 242 3 0.0 6 3 246 1 0.0 6 1 247 1 0.0 6 1 248 1 0.0 6 1 249 1 0.0 6 1 252 1 0.0 6 1 254 1 0.0 6 1 278 13 0.0 6 13 279 70 0.0 6 69 1 280 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1809 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 49 2 0.0 6 0 2 63 6 0.0 6 6 71 1 0.0 6 1 72 19 0.0 6 19 73 22 0.0 6 22 74 24 0.0 6 24 75 11 0.0 6 11 76 13 0.0 6 13 77 26 0.0 6 26 78 32 0.0 6 31 0 0 0 1 79 46 0.0 6 46 80 12 0.0 6 12 81 19 0.0 6 19 82 46 0.0 6 46 83 16 0.0 6 15 0 0 0 1 84 4 0.0 6 4 85 7 0.0 6 6 0 0 1 86 8 0.0 6 8 87 5 0.0 6 4 1 88 11 0.0 6 11 89 11 0.0 6 11 90 10 0.0 6 10 91 11 0.0 6 11 92 21 0.0 6 21 93 11 0.0 6 11 94 6 0.0 6 6 95 19 0.0 6 19 96 4 0.0 6 4 97 5 0.0 6 5 98 20 0.0 6 20 99 16 0.0 6 16 100 12 0.0 6 11 0 0 0 1 101 6 0.0 6 6 102 18 0.0 6 18 103 27 0.0 6 27 104 16 0.0 6 16 105 5 0.0 6 5 106 15 0.0 6 15 107 14 0.0 6 13 1 108 15 0.0 6 15 109 38 0.0 6 38 110 38 0.0 6 37 0 0 0 1 111 7 0.0 6 6 0 0 0 1 112 8 0.0 6 8 113 32 0.0 6 32 114 14 0.0 6 14 115 13 0.0 6 13 116 30 0.0 6 30 117 24 0.0 6 24 118 16 0.0 6 16 119 20 0.0 6 20 120 9 0.0 6 9 121 5 0.0 6 5 122 14 0.0 6 14 123 6 0.0 6 6 124 14 0.0 6 14 125 23 0.0 6 23 126 18 0.0 6 17 1 127 20 0.0 6 20 128 25 0.0 6 24 1 129 17 0.0 6 17 130 12 0.0 6 12 131 29 0.0 6 29 132 28 0.0 6 28 133 11 0.0 6 9 1 0 0 1 134 26 0.0 6 26 135 16 0.0 6 16 136 7 0.0 6 7 137 14 0.0 6 14 138 23 0.0 6 22 0 0 1 139 28 0.0 6 27 1 140 15 0.0 6 15 141 23 0.0 6 23 142 17 0.0 6 17 143 23 0.0 6 23 144 9 0.0 6 9 145 12 0.0 6 12 146 14 0.0 6 14 147 15 0.0 6 15 148 17 0.0 6 16 0 0 0 1 149 21 0.0 6 21 150 10 0.0 6 10 151 17 0.0 6 15 0 0 0 2 152 21 0.0 6 21 153 5 0.0 6 5 154 8 0.0 6 8 155 15 0.0 6 15 156 5 0.0 6 5 157 9 0.0 6 9 158 11 0.0 6 11 159 17 0.0 6 17 160 7 0.0 6 7 161 5 0.0 6 4 0 0 1 162 9 0.0 6 9 163 13 0.0 6 12 1 164 7 0.0 6 7 165 7 0.0 6 6 1 166 9 0.0 6 9 167 24 0.0 6 24 168 12 0.0 6 12 169 8 0.0 6 8 170 13 0.0 6 13 171 12 0.0 6 11 0 0 1 172 16 0.0 6 16 173 7 0.0 6 7 174 4 0.0 6 4 175 6 0.0 6 6 176 6 0.0 6 6 177 12 0.0 6 12 178 5 0.0 6 4 0 0 0 1 179 5 0.0 6 5 180 11 0.0 6 11 181 8 0.0 6 8 182 11 0.0 6 10 0 0 1 183 5 0.0 6 5 184 3 0.0 6 3 185 6 0.0 6 6 186 5 0.0 6 4 0 0 0 1 187 13 0.0 6 13 188 23 0.0 6 23 189 3 0.0 6 3 190 1 0.0 6 1 191 7 0.0 6 7 192 8 0.0 6 8 197 5 0.0 6 5 198 3 0.0 6 3 199 4 0.0 6 3 0 0 1 200 3 0.0 6 3 201 2 0.0 6 2 202 3 0.0 6 3 203 4 0.0 6 4 204 4 0.0 6 4 205 1 0.0 6 1 207 1 0.0 6 1 208 5 0.0 6 5 211 1 0.0 6 1 213 1 0.0 6 1 214 2 0.0 6 2 215 5 0.0 6 5 216 1 0.0 6 1 218 3 0.0 6 3 219 1 0.0 6 1 220 1 0.0 6 1 221 1 0.0 6 1 223 1 0.0 6 1 229 1 0.0 6 1 231 1 0.0 6 1 232 2 0.0 6 2 234 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_16_55_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_16_282_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_16_55_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_16_282_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.25 s (33 µs/read; 1.81 M reads/minute). === Summary === Total read pairs processed: 67,966 Read 1 with adapter: 67,966 (100.0%) Read 2 with adapter: 67,940 (100.0%) == Read fate breakdown == Pairs that were too short: 36 (0.1%) Pairs discarded as untrimmed: 26 (0.0%) Pairs written (passing filters): 67,904 (99.9%) Total basepairs processed: 29,547,012 bp Read 1: 15,975,390 bp Read 2: 13,571,622 bp Total written (filtered): 4,449,290 bp (15.1%) Read 1: 2,287,902 bp Read 2: 2,161,388 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 64911 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 1062.0 0 2 1 4 1 265.5 1 0 1 5 2 66.4 1 2 6 2 16.6 1 0 1 1 7 2 4.1 2 2 14 2 0.0 4 2 15 1 0.0 4 1 16 4 0.0 4 4 17 3 0.0 5 3 18 1 0.0 5 0 0 0 1 19 1 0.0 5 1 20 99 0.0 6 96 3 21 47 0.0 6 43 4 22 50 0.0 6 47 3 23 21 0.0 6 20 1 24 18 0.0 6 18 25 29 0.0 6 28 1 26 19 0.0 6 19 27 27 0.0 6 25 2 28 18 0.0 6 18 29 8 0.0 6 8 30 4 0.0 6 4 31 4 0.0 6 4 32 6 0.0 6 5 1 33 5 0.0 6 5 34 7 0.0 6 6 1 35 2 0.0 6 2 36 3 0.0 6 3 37 3 0.0 6 3 38 6 0.0 6 6 39 4 0.0 6 3 1 40 3 0.0 6 3 41 3 0.0 6 3 42 6 0.0 6 6 43 5 0.0 6 4 1 44 2 0.0 6 1 1 45 1 0.0 6 1 46 1 0.0 6 1 47 3 0.0 6 3 48 3 0.0 6 2 0 0 0 0 0 1 49 2 0.0 6 2 50 2 0.0 6 2 51 7 0.0 6 7 52 4 0.0 6 4 53 2 0.0 6 2 54 4 0.0 6 4 55 4 0.0 6 3 1 56 2 0.0 6 2 57 2 0.0 6 2 58 2 0.0 6 2 59 1 0.0 6 1 60 1 0.0 6 1 61 5 0.0 6 5 62 5 0.0 6 5 63 3 0.0 6 2 1 64 5 0.0 6 5 65 8 0.0 6 8 66 2 0.0 6 2 67 7 0.0 6 7 68 7 0.0 6 7 69 4 0.0 6 4 70 3 0.0 6 3 71 3 0.0 6 3 72 6 0.0 6 6 73 9 0.0 6 8 1 74 4 0.0 6 4 75 12 0.0 6 12 76 4 0.0 6 4 77 3 0.0 6 3 78 3 0.0 6 3 79 5 0.0 6 5 80 2 0.0 6 2 81 5 0.0 6 4 0 0 0 0 1 82 4 0.0 6 4 83 4 0.0 6 4 84 6 0.0 6 6 85 7 0.0 6 7 86 4 0.0 6 4 87 2 0.0 6 2 88 3 0.0 6 3 89 2 0.0 6 2 90 3 0.0 6 3 91 10 0.0 6 10 92 9 0.0 6 9 93 8 0.0 6 8 94 5 0.0 6 5 95 6 0.0 6 5 0 1 96 6 0.0 6 6 97 3 0.0 6 3 98 9 0.0 6 9 99 12 0.0 6 12 100 10 0.0 6 10 101 10 0.0 6 10 102 21 0.0 6 21 103 11 0.0 6 11 104 19 0.0 6 18 1 105 24 0.0 6 24 106 16 0.0 6 14 2 107 6 0.0 6 6 108 17 0.0 6 16 1 109 13 0.0 6 12 1 110 10 0.0 6 8 2 111 21 0.0 6 21 112 17 0.0 6 17 113 20 0.0 6 20 114 23 0.0 6 22 1 115 13 0.0 6 13 116 17 0.0 6 17 117 11 0.0 6 11 118 10 0.0 6 9 1 119 19 0.0 6 19 120 21 0.0 6 19 1 0 0 0 0 1 121 18 0.0 6 17 1 122 21 0.0 6 17 4 123 24 0.0 6 24 124 18 0.0 6 17 1 125 23 0.0 6 23 126 47 0.0 6 47 127 27 0.0 6 27 128 45 0.0 6 44 0 0 0 0 0 1 129 37 0.0 6 37 130 30 0.0 6 29 1 131 31 0.0 6 31 132 25 0.0 6 25 133 40 0.0 6 39 0 0 0 0 1 134 70 0.0 6 69 1 135 40 0.0 6 40 136 48 0.0 6 48 137 63 0.0 6 62 1 138 33 0.0 6 33 139 60 0.0 6 59 1 140 70 0.0 6 67 3 141 415 0.0 6 413 2 142 1202 0.0 6 1202 143 227 0.0 6 226 1 144 275 0.0 6 274 1 145 190 0.0 6 189 1 146 1366 0.0 6 1362 4 147 1362 0.0 6 1360 2 148 285 0.0 6 282 2 0 0 0 1 149 1379 0.0 6 1375 2 0 0 1 1 150 259 0.0 6 258 1 151 83 0.0 6 83 152 169 0.0 6 168 1 153 1254 0.0 6 1252 2 154 187 0.0 6 187 155 58 0.0 6 57 1 156 91 0.0 6 90 1 157 112 0.0 6 107 4 0 1 158 190 0.0 6 186 4 159 1184 0.0 6 1183 1 160 239 0.0 6 239 161 242 0.0 6 241 1 162 1777 0.0 6 1776 1 163 342 0.0 6 339 1 1 0 1 164 128 0.0 6 127 1 165 149 0.0 6 147 2 166 289 0.0 6 284 5 167 1718 0.0 6 1715 3 168 1429 0.0 6 1421 7 0 0 1 169 380 0.0 6 378 2 170 3035 0.0 6 3030 5 171 457 0.0 6 457 172 63 0.0 6 62 0 0 0 0 0 1 173 49 0.0 6 49 174 39 0.0 6 39 175 77 0.0 6 76 0 1 176 57 0.0 6 56 1 177 67 0.0 6 64 2 0 1 178 46 0.0 6 46 179 61 0.0 6 59 1 0 1 180 115 0.0 6 114 1 181 215 0.0 6 212 3 182 82 0.0 6 80 2 183 54 0.0 6 53 1 184 43 0.0 6 42 1 185 51 0.0 6 51 186 63 0.0 6 63 187 72 0.0 6 71 1 188 58 0.0 6 57 1 189 59 0.0 6 58 1 190 93 0.0 6 92 0 0 0 0 1 191 153 0.0 6 153 192 79 0.0 6 78 1 193 157 0.0 6 155 2 194 101 0.0 6 97 4 195 181 0.0 6 179 2 196 343 0.0 6 342 1 197 1378 0.0 6 1376 2 198 366 0.0 6 361 5 199 236 0.0 6 234 2 200 180 0.0 6 177 2 0 1 201 193 0.0 6 188 4 0 1 202 344 0.0 6 342 2 203 233 0.0 6 233 204 408 0.0 6 403 5 205 741 0.0 6 739 2 206 304 0.0 6 303 1 207 495 0.0 6 489 6 208 520 0.0 6 519 1 209 812 0.0 6 809 2 0 0 0 0 1 210 531 0.0 6 530 1 211 558 0.0 6 556 2 212 882 0.0 6 872 8 1 0 0 1 213 1509 0.0 6 1499 9 0 0 0 0 1 214 4861 0.0 6 4836 25 215 738 0.0 6 733 4 0 1 216 473 0.0 6 471 2 217 320 0.0 6 319 1 218 294 0.0 6 294 219 305 0.0 6 302 3 220 392 0.0 6 392 221 356 0.0 6 353 3 222 1187 0.0 6 1184 1 1 1 223 157 0.0 6 153 3 0 1 224 175 0.0 6 173 2 225 206 0.0 6 204 2 226 455 0.0 6 453 2 227 337 0.0 6 332 5 228 303 0.0 6 298 5 229 239 0.0 6 237 2 230 279 0.0 6 276 3 231 390 0.0 6 390 232 537 0.0 6 532 5 233 792 0.0 6 791 1 234 709 0.0 6 708 1 235 519 0.0 6 518 1 236 581 0.0 6 578 3 237 784 0.0 6 783 1 238 1638 0.0 6 1633 5 239 315 0.0 6 313 2 240 313 0.0 6 311 2 241 498 0.0 6 496 2 242 295 0.0 6 293 2 243 323 0.0 6 323 244 218 0.0 6 218 245 207 0.0 6 206 0 0 1 246 248 0.0 6 248 247 333 0.0 6 332 1 248 580 0.0 6 579 1 249 270 0.0 6 270 250 251 0.0 6 251 251 255 0.0 6 254 1 252 227 0.0 6 226 1 253 200 0.0 6 200 254 242 0.0 6 242 255 338 0.0 6 337 1 256 114 0.0 6 113 1 257 94 0.0 6 92 2 258 96 0.0 6 95 1 259 135 0.0 6 133 2 260 152 0.0 6 152 261 52 0.0 6 51 1 262 64 0.0 6 64 263 101 0.0 6 101 264 124 0.0 6 124 265 77 0.0 6 77 266 119 0.0 6 119 267 91 0.0 6 91 268 100 0.0 6 100 269 174 0.0 6 173 1 270 118 0.0 6 116 1 1 271 172 0.0 6 172 272 272 0.0 6 272 273 88 0.0 6 88 274 112 0.0 6 112 275 241 0.0 6 240 0 1 276 167 0.0 6 167 277 160 0.0 6 160 278 348 0.0 6 346 2 279 939 0.0 6 931 6 0 1 1 280 4317 0.0 6 4302 12 0 0 1 2 281 31 0.0 6 31 282 2 0.0 6 2 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 3055 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 8 265.5 1 0 8 70 3 0.0 6 3 71 29 0.0 6 28 1 72 69 0.0 6 69 73 4 0.0 6 4 74 11 0.0 6 11 75 17 0.0 6 17 76 79 0.0 6 78 1 77 90 0.0 6 90 78 25 0.0 6 25 79 71 0.0 6 70 1 80 7 0.0 6 7 82 21 0.0 6 19 2 83 63 0.0 6 62 1 84 3 0.0 6 3 85 1 0.0 6 1 88 14 0.0 6 14 89 58 0.0 6 58 90 4 0.0 6 4 91 19 0.0 6 19 92 93 0.0 6 93 93 5 0.0 6 5 95 1 0.0 6 1 96 18 0.0 6 18 97 90 0.0 6 89 1 98 67 0.0 6 67 99 42 0.0 6 42 100 188 0.0 6 187 1 101 7 0.0 6 7 105 1 0.0 6 1 108 1 0.0 6 1 120 3 0.0 6 3 121 8 0.0 6 8 123 2 0.0 6 2 126 17 0.0 6 17 127 97 0.0 6 97 128 3 0.0 6 2 1 132 1 0.0 6 1 133 1 0.0 6 1 134 1 0.0 6 1 139 2 0.0 6 2 140 1 0.0 6 1 141 3 0.0 6 3 142 5 0.0 6 4 1 143 160 0.0 6 160 144 574 0.0 6 573 1 145 18 0.0 6 18 146 1 0.0 6 1 160 1 0.0 6 1 161 1 0.0 6 1 164 2 0.0 6 2 165 3 0.0 6 3 166 5 0.0 6 5 167 83 0.0 6 83 168 279 0.0 6 278 1 169 8 0.0 6 8 172 2 0.0 6 2 173 6 0.0 6 6 174 1 0.0 6 1 175 4 0.0 6 4 176 11 0.0 6 11 183 1 0.0 6 1 184 2 0.0 6 2 185 15 0.0 6 15 186 1 0.0 6 1 187 1 0.0 6 1 188 2 0.0 6 2 189 15 0.0 6 15 190 17 0.0 6 17 191 1 0.0 6 1 195 1 0.0 6 1 196 5 0.0 6 5 197 12 0.0 6 12 201 1 0.0 6 1 203 7 0.0 6 7 204 40 0.0 6 40 205 2 0.0 6 2 206 1 0.0 6 1 207 2 0.0 6 2 208 2 0.0 6 2 209 1 0.0 6 1 210 74 0.0 6 74 211 216 0.0 6 216 212 84 0.0 6 83 1 213 2 0.0 6 2 218 1 0.0 6 1 219 4 0.0 6 4 226 1 0.0 6 1 234 7 0.0 6 7 235 18 0.0 6 18 236 1 0.0 6 1 237 1 0.0 6 1 238 15 0.0 6 15 239 1 0.0 6 0 1 270 1 0.0 6 1 272 17 0.0 6 17 273 45 0.0 6 45 274 3 0.0 6 3 280 1 0.0 6 1 281 5 0.0 6 5 282 13 0.0 6 13 283 4 0.0 6 4 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 65152 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 4.1 2 1 9 1 0.3 2 1 11 3 0.0 3 2 0 1 12 1 0.0 3 1 13 7 0.0 3 7 14 1 0.0 4 1 16 14 0.0 4 12 1 0 1 17 6 0.0 5 6 18 8 0.0 5 8 19 10 0.0 5 8 1 1 20 73 0.0 6 70 3 21 42 0.0 6 40 2 22 25 0.0 6 22 3 23 21 0.0 6 19 2 24 7 0.0 6 6 1 25 6 0.0 6 5 1 26 14 0.0 6 12 1 1 27 14 0.0 6 14 28 10 0.0 6 10 29 10 0.0 6 10 30 17 0.0 6 16 0 1 31 15 0.0 6 14 0 1 32 5 0.0 6 5 33 15 0.0 6 15 34 5 0.0 6 5 35 3 0.0 6 3 36 6 0.0 6 6 37 6 0.0 6 6 38 15 0.0 6 13 1 0 1 39 11 0.0 6 10 0 1 40 6 0.0 6 5 1 41 6 0.0 6 5 0 0 1 42 6 0.0 6 5 1 43 10 0.0 6 10 44 7 0.0 6 7 45 2 0.0 6 2 46 4 0.0 6 4 47 8 0.0 6 7 0 0 1 48 9 0.0 6 8 1 49 9 0.0 6 9 50 6 0.0 6 5 1 51 8 0.0 6 6 2 52 6 0.0 6 6 53 8 0.0 6 5 3 54 18 0.0 6 18 55 15 0.0 6 14 1 56 15 0.0 6 12 3 57 13 0.0 6 12 1 58 11 0.0 6 11 59 16 0.0 6 16 60 12 0.0 6 11 1 61 20 0.0 6 17 1 0 2 62 13 0.0 6 12 1 63 13 0.0 6 13 64 11 0.0 6 11 65 15 0.0 6 14 1 66 16 0.0 6 15 1 67 7 0.0 6 5 0 1 0 1 68 40 0.0 6 36 3 0 0 1 69 126 0.0 6 122 3 0 1 70 18 0.0 6 18 71 61 0.0 6 58 2 0 0 0 0 1 72 20 0.0 6 18 1 1 73 33 0.0 6 32 1 74 17 0.0 6 17 75 19 0.0 6 17 2 76 31 0.0 6 28 3 77 19 0.0 6 17 2 78 28 0.0 6 27 1 79 22 0.0 6 21 1 80 23 0.0 6 21 1 0 0 1 81 20 0.0 6 18 2 82 36 0.0 6 35 1 83 35 0.0 6 33 1 0 0 1 84 41 0.0 6 40 0 0 1 85 37 0.0 6 34 2 0 0 0 1 86 32 0.0 6 30 0 0 2 87 16 0.0 6 14 2 88 21 0.0 6 21 89 19 0.0 6 19 90 33 0.0 6 32 1 91 16 0.0 6 16 92 25 0.0 6 25 93 27 0.0 6 27 94 20 0.0 6 17 1 0 2 95 31 0.0 6 30 0 1 96 28 0.0 6 26 0 0 2 97 30 0.0 6 30 98 54 0.0 6 52 1 0 1 99 56 0.0 6 53 3 100 48 0.0 6 47 1 101 37 0.0 6 36 1 102 33 0.0 6 31 2 103 42 0.0 6 41 1 104 47 0.0 6 47 105 45 0.0 6 44 1 106 87 0.0 6 83 4 107 51 0.0 6 49 1 0 1 108 47 0.0 6 42 3 1 1 109 54 0.0 6 51 2 0 1 110 49 0.0 6 48 1 111 61 0.0 6 60 1 112 67 0.0 6 65 2 113 77 0.0 6 76 1 114 68 0.0 6 67 1 115 77 0.0 6 75 2 116 56 0.0 6 56 117 70 0.0 6 69 1 118 52 0.0 6 49 3 119 54 0.0 6 53 1 120 117 0.0 6 115 2 121 74 0.0 6 74 122 82 0.0 6 77 4 1 123 91 0.0 6 91 124 101 0.0 6 100 1 125 104 0.0 6 103 1 126 94 0.0 6 94 127 135 0.0 6 133 2 128 144 0.0 6 142 2 129 98 0.0 6 96 2 130 160 0.0 6 157 2 1 131 124 0.0 6 123 1 132 166 0.0 6 163 3 133 167 0.0 6 161 6 134 193 0.0 6 188 5 135 219 0.0 6 215 4 136 254 0.0 6 250 4 137 344 0.0 6 342 2 138 342 0.0 6 336 6 139 534 0.0 6 528 6 140 1163 0.0 6 1160 1 1 0 1 141 477 0.0 6 473 4 142 553 0.0 6 552 1 143 734 0.0 6 724 5 4 0 0 0 1 144 1585 0.0 6 1579 4 2 145 1257 0.0 6 1251 6 146 622 0.0 6 615 6 0 0 0 0 1 147 1290 0.0 6 1282 6 0 1 0 0 1 148 641 0.0 6 631 8 2 149 513 0.0 6 509 4 150 647 0.0 6 637 9 0 1 151 1486 0.0 6 1478 8 152 862 0.0 6 846 15 0 1 153 521 0.0 6 507 11 1 2 154 807 0.0 6 793 13 0 1 155 759 0.0 6 748 7 2 2 156 792 0.0 6 778 13 1 157 1304 0.0 6 1297 7 158 702 0.0 6 691 10 0 1 159 856 0.0 6 850 5 0 0 1 160 1437 0.0 6 1427 9 1 161 872 0.0 6 866 6 162 663 0.0 6 659 4 163 777 0.0 6 763 12 2 164 784 0.0 6 771 10 2 1 165 1279 0.0 6 1273 6 166 1096 0.0 6 1082 10 2 1 1 167 825 0.0 6 809 13 1 1 1 168 1861 0.0 6 1855 5 1 169 596 0.0 6 580 14 2 170 583 0.0 6 575 7 1 171 458 0.0 6 448 8 0 1 0 0 1 172 614 0.0 6 609 5 173 541 0.0 6 532 6 1 1 0 0 1 174 690 0.0 6 669 18 1 2 175 688 0.0 6 673 14 1 176 765 0.0 6 743 19 2 1 177 610 0.0 6 588 18 2 2 178 974 0.0 6 964 8 2 179 525 0.0 6 518 6 0 1 180 791 0.0 6 787 3 0 0 0 0 1 181 549 0.0 6 547 2 182 640 0.0 6 639 1 183 630 0.0 6 628 1 0 1 184 322 0.0 6 320 2 185 964 0.0 6 964 186 1185 0.0 6 1180 4 0 1 187 956 0.0 6 950 4 0 2 188 737 0.0 6 735 2 189 655 0.0 6 651 4 190 642 0.0 6 637 4 0 0 0 0 1 191 703 0.0 6 698 4 0 1 192 634 0.0 6 629 4 1 193 662 0.0 6 661 1 194 610 0.0 6 608 2 195 1097 0.0 6 1093 3 0 1 196 716 0.0 6 711 4 0 1 197 415 0.0 6 414 1 198 456 0.0 6 454 1 0 1 199 553 0.0 6 553 200 395 0.0 6 390 5 201 372 0.0 6 371 0 0 0 0 0 1 202 570 0.0 6 565 3 1 0 0 1 203 369 0.0 6 368 1 204 469 0.0 6 467 2 205 391 0.0 6 388 1 0 2 206 508 0.0 6 505 3 207 367 0.0 6 364 2 1 208 395 0.0 6 393 2 209 637 0.0 6 636 1 210 552 0.0 6 551 1 211 664 0.0 6 659 4 0 1 212 770 0.0 6 770 213 524 0.0 6 521 3 214 443 0.0 6 443 215 147 0.0 6 146 1 216 209 0.0 6 207 2 217 211 0.0 6 208 3 218 202 0.0 6 200 2 219 269 0.0 6 268 1 220 284 0.0 6 282 2 221 223 0.0 6 222 1 222 73 0.0 6 73 223 149 0.0 6 146 2 1 224 129 0.0 6 128 1 225 149 0.0 6 148 1 226 61 0.0 6 61 227 80 0.0 6 80 228 140 0.0 6 139 1 229 44 0.0 6 44 230 74 0.0 6 73 1 231 41 0.0 6 41 232 65 0.0 6 65 233 88 0.0 6 88 234 56 0.0 6 56 235 55 0.0 6 55 236 111 0.0 6 111 237 33 0.0 6 33 238 18 0.0 6 16 1 0 0 0 1 239 30 0.0 6 30 240 25 0.0 6 24 1 241 13 0.0 6 13 242 6 0.0 6 6 243 18 0.0 6 17 1 244 31 0.0 6 31 245 19 0.0 6 19 246 19 0.0 6 19 247 8 0.0 6 8 248 25 0.0 6 25 249 9 0.0 6 9 250 8 0.0 6 8 251 8 0.0 6 8 252 11 0.0 6 11 253 16 0.0 6 16 254 1 0.0 6 1 255 2 0.0 6 2 277 1 0.0 6 1 279 4 0.0 6 3 1 280 11 0.0 6 10 1 281 63 0.0 6 62 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2788 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 56 1 0.0 6 1 67 1 0.0 6 1 69 7 0.0 6 6 1 70 24 0.0 6 23 1 71 64 0.0 6 64 72 5 0.0 6 5 73 8 0.0 6 8 74 17 0.0 6 17 75 80 0.0 6 80 76 72 0.0 6 71 1 77 9 0.0 6 9 78 62 0.0 6 61 0 0 0 1 79 5 0.0 6 5 80 4 0.0 6 2 0 0 0 2 81 7 0.0 6 7 82 65 0.0 6 64 0 0 0 1 84 1 0.0 6 1 85 1 0.0 6 1 86 2 0.0 6 2 87 11 0.0 6 11 88 51 0.0 6 51 89 1 0.0 6 1 90 16 0.0 6 16 91 108 0.0 6 108 92 2 0.0 6 2 93 1 0.0 6 1 94 3 0.0 6 3 95 20 0.0 6 19 0 0 0 1 96 89 0.0 6 89 97 74 0.0 6 74 98 37 0.0 6 35 1 0 0 1 99 166 0.0 6 166 100 9 0.0 6 5 0 0 0 4 101 1 0.0 6 0 0 0 0 1 102 1 0.0 6 0 0 0 1 103 1 0.0 6 0 0 0 0 1 104 1 0.0 6 1 119 1 0.0 6 1 120 6 0.0 6 6 122 3 0.0 6 3 124 3 0.0 6 3 125 13 0.0 6 13 126 67 0.0 6 67 130 2 0.0 6 1 0 0 0 1 131 2 0.0 6 2 133 2 0.0 6 2 142 19 0.0 6 19 143 926 0.0 6 924 2 144 22 0.0 6 20 2 146 2 0.0 6 0 0 0 2 147 12 0.0 6 0 0 0 0 12 162 1 0.0 6 1 164 2 0.0 6 2 165 13 0.0 6 12 1 166 47 0.0 6 47 167 263 0.0 6 263 168 6 0.0 6 5 1 170 2 0.0 6 0 0 0 0 2 171 6 0.0 6 0 0 0 0 6 172 4 0.0 6 4 174 2 0.0 6 2 175 5 0.0 6 5 181 1 0.0 6 1 183 1 0.0 6 1 184 13 0.0 6 13 185 1 0.0 6 1 187 1 0.0 6 1 188 6 0.0 6 6 189 17 0.0 6 17 190 3 0.0 6 3 195 1 0.0 6 1 196 12 0.0 6 12 201 4 0.0 6 3 1 202 5 0.0 6 5 203 27 0.0 6 27 206 2 0.0 6 2 208 4 0.0 6 3 1 209 38 0.0 6 38 210 144 0.0 6 144 211 18 0.0 6 17 1 214 5 0.0 6 1 0 0 0 4 233 7 0.0 6 7 234 16 0.0 6 16 237 4 0.0 6 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_17_56_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_17_283_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_17_56_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_17_283_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.47 s (22 µs/read; 2.74 M reads/minute). === Summary === Total read pairs processed: 67,442 Read 1 with adapter: 67,442 (100.0%) Read 2 with adapter: 67,416 (100.0%) == Read fate breakdown == Pairs that were too short: 18 (0.0%) Pairs discarded as untrimmed: 26 (0.0%) Pairs written (passing filters): 67,398 (99.9%) Total basepairs processed: 27,485,990 bp Read 1: 14,924,743 bp Read 2: 12,561,247 bp Total written (filtered): 4,252,653 bp (15.5%) Read 1: 2,220,447 bp Read 2: 2,032,206 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 65141 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1053.8 0 1 1 4 6 263.4 1 4 2 5 1 65.9 1 1 6 3 16.5 1 0 0 3 8 1 1.0 2 1 9 1 0.3 2 1 10 2 0.1 3 2 15 3 0.0 4 3 16 5 0.0 4 3 2 17 2 0.0 5 1 1 19 4 0.0 5 3 1 20 114 0.0 6 112 2 21 68 0.0 6 66 2 22 75 0.0 6 72 3 23 32 0.0 6 31 1 24 32 0.0 6 32 25 22 0.0 6 21 1 26 8 0.0 6 8 27 11 0.0 6 10 1 28 11 0.0 6 11 29 11 0.0 6 10 1 30 7 0.0 6 7 31 9 0.0 6 8 1 32 6 0.0 6 6 33 9 0.0 6 9 34 9 0.0 6 8 0 0 0 0 0 1 35 3 0.0 6 3 36 6 0.0 6 5 1 37 6 0.0 6 6 38 6 0.0 6 5 1 39 11 0.0 6 10 1 40 8 0.0 6 8 41 3 0.0 6 3 42 8 0.0 6 7 1 43 10 0.0 6 10 44 2 0.0 6 2 45 8 0.0 6 7 1 46 10 0.0 6 9 1 47 4 0.0 6 3 1 48 8 0.0 6 8 49 6 0.0 6 6 50 5 0.0 6 3 2 51 10 0.0 6 10 52 6 0.0 6 4 0 0 0 1 0 1 53 7 0.0 6 7 54 3 0.0 6 3 55 6 0.0 6 6 57 4 0.0 6 4 58 8 0.0 6 8 59 2 0.0 6 2 60 5 0.0 6 4 0 1 61 6 0.0 6 6 62 5 0.0 6 5 63 8 0.0 6 8 64 9 0.0 6 8 1 65 6 0.0 6 6 66 8 0.0 6 7 1 67 6 0.0 6 6 68 8 0.0 6 8 69 8 0.0 6 8 70 8 0.0 6 8 71 15 0.0 6 14 0 0 0 0 0 1 72 11 0.0 6 11 73 5 0.0 6 5 74 5 0.0 6 5 75 8 0.0 6 7 1 76 12 0.0 6 12 77 9 0.0 6 8 0 0 0 0 1 78 6 0.0 6 6 79 3 0.0 6 3 80 4 0.0 6 4 81 12 0.0 6 11 1 82 9 0.0 6 9 83 9 0.0 6 9 84 18 0.0 6 18 85 5 0.0 6 2 2 0 0 0 0 1 86 10 0.0 6 10 87 11 0.0 6 10 1 88 14 0.0 6 13 0 1 89 8 0.0 6 7 1 90 15 0.0 6 15 91 7 0.0 6 6 1 92 3 0.0 6 3 93 8 0.0 6 7 1 94 9 0.0 6 9 95 14 0.0 6 12 2 96 7 0.0 6 6 1 97 6 0.0 6 6 98 10 0.0 6 9 1 99 7 0.0 6 7 100 12 0.0 6 12 101 16 0.0 6 15 1 102 14 0.0 6 14 103 21 0.0 6 21 104 20 0.0 6 20 105 17 0.0 6 17 106 28 0.0 6 27 1 107 15 0.0 6 15 108 18 0.0 6 18 109 13 0.0 6 13 110 17 0.0 6 16 1 111 28 0.0 6 27 1 112 17 0.0 6 17 113 32 0.0 6 31 1 114 31 0.0 6 30 1 115 23 0.0 6 23 116 37 0.0 6 37 117 25 0.0 6 23 1 0 0 0 1 118 26 0.0 6 25 1 119 28 0.0 6 28 120 29 0.0 6 29 121 28 0.0 6 28 122 22 0.0 6 21 1 123 32 0.0 6 32 124 42 0.0 6 42 125 33 0.0 6 33 126 29 0.0 6 28 0 0 0 1 127 28 0.0 6 28 128 34 0.0 6 34 129 39 0.0 6 39 130 47 0.0 6 47 131 51 0.0 6 51 132 51 0.0 6 51 133 49 0.0 6 49 134 37 0.0 6 37 135 52 0.0 6 52 136 56 0.0 6 55 1 137 65 0.0 6 64 1 138 47 0.0 6 47 139 77 0.0 6 76 1 140 330 0.0 6 330 141 645 0.0 6 645 142 557 0.0 6 555 2 143 306 0.0 6 304 2 144 677 0.0 6 674 1 0 1 0 0 1 145 501 0.0 6 497 4 146 514 0.0 6 513 1 147 631 0.0 6 628 3 148 549 0.0 6 548 1 149 690 0.0 6 687 3 150 802 0.0 6 801 1 151 509 0.0 6 509 152 561 0.0 6 559 2 153 642 0.0 6 641 1 154 476 0.0 6 473 3 155 308 0.0 6 307 1 156 391 0.0 6 391 157 649 0.0 6 648 1 158 843 0.0 6 840 3 159 508 0.0 6 508 160 538 0.0 6 535 2 1 161 734 0.0 6 734 162 523 0.0 6 522 1 163 653 0.0 6 649 4 164 713 0.0 6 712 1 165 723 0.0 6 721 1 0 0 0 0 1 166 1061 0.0 6 1060 1 167 462 0.0 6 459 3 168 617 0.0 6 613 2 1 0 1 169 452 0.0 6 451 1 170 1506 0.0 6 1503 3 171 975 0.0 6 972 3 172 496 0.0 6 496 173 476 0.0 6 473 2 0 0 1 174 262 0.0 6 262 175 514 0.0 6 514 176 418 0.0 6 417 1 177 330 0.0 6 329 0 1 178 1038 0.0 6 1034 3 0 0 0 1 179 654 0.0 6 650 3 0 0 0 0 1 180 533 0.0 6 532 1 181 315 0.0 6 315 182 325 0.0 6 325 183 390 0.0 6 388 2 184 549 0.0 6 548 1 185 451 0.0 6 450 1 186 377 0.0 6 375 2 187 646 0.0 6 645 1 188 623 0.0 6 622 1 189 319 0.0 6 318 0 1 190 984 0.0 6 981 3 191 412 0.0 6 409 3 192 390 0.0 6 386 3 1 193 651 0.0 6 646 5 194 699 0.0 6 697 2 195 660 0.0 6 659 1 196 1104 0.0 6 1099 5 197 865 0.0 6 863 2 198 1339 0.0 6 1337 2 199 1080 0.0 6 1076 4 200 669 0.0 6 665 3 1 201 607 0.0 6 604 3 202 567 0.0 6 564 3 203 438 0.0 6 435 2 0 0 1 204 528 0.0 6 528 205 600 0.0 6 600 206 567 0.0 6 566 1 207 1012 0.0 6 1007 4 0 0 1 208 581 0.0 6 576 4 0 0 0 1 209 668 0.0 6 661 6 0 0 1 210 462 0.0 6 456 4 2 211 506 0.0 6 503 3 212 519 0.0 6 518 1 213 520 0.0 6 518 2 214 580 0.0 6 575 4 1 215 517 0.0 6 509 6 1 1 216 748 0.0 6 744 4 217 901 0.0 6 899 2 218 557 0.0 6 553 3 0 0 0 0 1 219 548 0.0 6 542 5 1 220 475 0.0 6 471 4 221 486 0.0 6 480 6 222 446 0.0 6 444 2 223 455 0.0 6 452 3 224 420 0.0 6 419 1 225 445 0.0 6 441 4 226 524 0.0 6 522 1 0 1 227 490 0.0 6 487 3 228 385 0.0 6 385 229 453 0.0 6 453 230 395 0.0 6 394 1 231 342 0.0 6 341 1 232 388 0.0 6 386 2 233 353 0.0 6 351 2 234 348 0.0 6 344 4 235 360 0.0 6 359 0 1 236 310 0.0 6 309 1 237 251 0.0 6 248 2 0 0 1 238 237 0.0 6 234 3 239 264 0.0 6 264 240 354 0.0 6 349 4 0 0 0 0 1 241 220 0.0 6 219 1 242 260 0.0 6 259 1 243 241 0.0 6 237 2 2 244 228 0.0 6 225 3 245 173 0.0 6 173 246 174 0.0 6 172 2 247 154 0.0 6 151 3 248 147 0.0 6 145 1 1 249 159 0.0 6 158 1 250 151 0.0 6 149 2 251 142 0.0 6 141 1 252 150 0.0 6 148 2 253 122 0.0 6 120 1 0 1 254 97 0.0 6 97 255 121 0.0 6 120 1 256 99 0.0 6 97 2 257 111 0.0 6 111 258 93 0.0 6 93 259 90 0.0 6 90 260 117 0.0 6 116 1 261 106 0.0 6 102 4 262 94 0.0 6 92 2 263 79 0.0 6 79 264 68 0.0 6 67 1 265 70 0.0 6 70 266 80 0.0 6 80 267 85 0.0 6 84 1 268 74 0.0 6 73 1 269 61 0.0 6 61 270 77 0.0 6 77 271 76 0.0 6 76 272 92 0.0 6 88 3 0 0 0 1 273 67 0.0 6 65 2 274 63 0.0 6 62 1 275 73 0.0 6 72 0 1 276 123 0.0 6 123 277 148 0.0 6 146 1 0 1 278 132 0.0 6 132 279 381 0.0 6 379 2 280 687 0.0 6 684 3 281 6 0.0 6 5 1 282 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2301 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 11 263.4 1 0 11 61 4 0.0 6 3 1 62 114 0.0 6 113 1 63 3 0.0 6 2 1 69 1 0.0 6 1 70 4 0.0 6 4 71 17 0.0 6 17 72 23 0.0 6 23 73 23 0.0 6 23 74 8 0.0 6 8 75 27 0.0 6 27 76 12 0.0 6 12 77 22 0.0 6 22 78 23 0.0 6 23 79 19 0.0 6 19 80 27 0.0 6 27 81 37 0.0 6 37 82 21 0.0 6 21 83 17 0.0 6 17 84 21 0.0 6 21 85 9 0.0 6 9 86 8 0.0 6 8 87 17 0.0 6 17 88 28 0.0 6 28 89 22 0.0 6 22 90 18 0.0 6 17 1 91 25 0.0 6 25 92 33 0.0 6 33 93 21 0.0 6 21 94 28 0.0 6 28 95 23 0.0 6 23 96 31 0.0 6 31 97 44 0.0 6 44 98 18 0.0 6 18 99 27 0.0 6 27 100 39 0.0 6 38 0 0 0 0 0 1 101 56 0.0 6 55 1 102 30 0.0 6 29 1 103 15 0.0 6 15 104 13 0.0 6 13 105 8 0.0 6 8 106 17 0.0 6 17 107 11 0.0 6 11 108 10 0.0 6 10 109 45 0.0 6 45 110 15 0.0 6 15 111 17 0.0 6 17 112 7 0.0 6 7 113 10 0.0 6 10 114 11 0.0 6 11 115 15 0.0 6 15 116 11 0.0 6 11 117 13 0.0 6 13 118 17 0.0 6 17 119 22 0.0 6 22 120 9 0.0 6 9 121 34 0.0 6 34 122 4 0.0 6 4 123 10 0.0 6 10 124 18 0.0 6 18 125 18 0.0 6 18 126 21 0.0 6 21 127 30 0.0 6 30 128 16 0.0 6 16 129 46 0.0 6 46 130 24 0.0 6 24 131 18 0.0 6 18 132 15 0.0 6 15 133 11 0.0 6 11 134 13 0.0 6 12 0 0 0 0 0 1 135 21 0.0 6 21 136 14 0.0 6 14 137 12 0.0 6 12 138 28 0.0 6 28 139 19 0.0 6 19 140 18 0.0 6 18 141 13 0.0 6 13 142 21 0.0 6 21 143 14 0.0 6 14 144 11 0.0 6 11 145 5 0.0 6 5 147 15 0.0 6 15 148 37 0.0 6 37 149 11 0.0 6 11 150 2 0.0 6 2 151 8 0.0 6 8 152 19 0.0 6 19 153 6 0.0 6 6 154 9 0.0 6 9 155 8 0.0 6 8 156 10 0.0 6 10 157 14 0.0 6 14 158 6 0.0 6 6 159 5 0.0 6 5 160 17 0.0 6 17 161 21 0.0 6 21 162 18 0.0 6 18 163 28 0.0 6 28 164 11 0.0 6 11 165 7 0.0 6 7 166 7 0.0 6 7 167 8 0.0 6 8 168 4 0.0 6 4 169 3 0.0 6 3 170 11 0.0 6 11 171 27 0.0 6 27 172 1 0.0 6 1 173 10 0.0 6 10 174 1 0.0 6 1 175 8 0.0 6 8 176 6 0.0 6 6 177 3 0.0 6 3 178 6 0.0 6 6 179 2 0.0 6 2 180 6 0.0 6 6 181 4 0.0 6 4 182 7 0.0 6 7 183 9 0.0 6 9 184 6 0.0 6 6 185 5 0.0 6 5 186 7 0.0 6 7 187 9 0.0 6 9 188 10 0.0 6 10 189 11 0.0 6 11 190 10 0.0 6 10 191 2 0.0 6 2 192 8 0.0 6 8 193 9 0.0 6 9 194 4 0.0 6 4 195 3 0.0 6 3 196 1 0.0 6 1 197 1 0.0 6 1 198 5 0.0 6 5 199 10 0.0 6 10 200 15 0.0 6 15 201 9 0.0 6 9 202 2 0.0 6 2 203 2 0.0 6 2 204 5 0.0 6 5 205 5 0.0 6 5 206 3 0.0 6 3 207 8 0.0 6 8 208 13 0.0 6 13 210 5 0.0 6 5 211 4 0.0 6 4 212 1 0.0 6 1 213 7 0.0 6 7 214 3 0.0 6 3 215 5 0.0 6 5 216 2 0.0 6 2 217 3 0.0 6 3 218 3 0.0 6 3 220 3 0.0 6 3 222 4 0.0 6 4 224 3 0.0 6 3 225 2 0.0 6 2 226 7 0.0 6 7 227 2 0.0 6 2 228 3 0.0 6 3 229 1 0.0 6 1 230 6 0.0 6 6 231 7 0.0 6 7 232 3 0.0 6 3 233 1 0.0 6 1 234 2 0.0 6 2 235 3 0.0 6 3 236 2 0.0 6 2 237 1 0.0 6 1 238 3 0.0 6 3 239 2 0.0 6 2 241 2 0.0 6 2 243 1 0.0 6 1 245 2 0.0 6 2 247 2 0.0 6 2 248 1 0.0 6 1 249 1 0.0 6 1 250 2 0.0 6 2 251 2 0.0 6 2 252 1 0.0 6 1 253 3 0.0 6 3 254 4 0.0 6 4 255 1 0.0 6 1 256 2 0.0 6 2 260 1 0.0 6 1 262 1 0.0 6 1 263 1 0.0 6 1 264 1 0.0 6 1 265 4 0.0 6 4 267 2 0.0 6 2 270 1 0.0 6 1 273 1 0.0 6 1 275 1 0.0 6 1 278 1 0.0 6 1 284 1 0.0 6 1 285 2 0.0 6 2 306 1 0.0 6 1 307 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 65554 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1053.8 0 1 6 1 16.5 1 1 10 3 0.1 3 2 1 11 2 0.0 3 2 12 3 0.0 3 3 13 3 0.0 3 3 14 9 0.0 4 8 0 1 15 2 0.0 4 2 16 2 0.0 4 2 17 13 0.0 5 10 3 18 4 0.0 5 3 1 19 37 0.0 5 32 5 20 80 0.0 6 75 4 1 21 36 0.0 6 35 1 22 32 0.0 6 31 1 23 28 0.0 6 27 1 24 16 0.0 6 16 25 18 0.0 6 15 2 0 0 1 26 9 0.0 6 8 1 27 13 0.0 6 13 28 23 0.0 6 22 0 1 29 8 0.0 6 7 1 30 12 0.0 6 12 31 17 0.0 6 13 3 1 32 10 0.0 6 9 1 33 8 0.0 6 7 1 34 9 0.0 6 8 1 35 13 0.0 6 11 2 36 7 0.0 6 7 37 7 0.0 6 7 38 9 0.0 6 7 2 39 9 0.0 6 8 0 0 1 40 8 0.0 6 8 41 6 0.0 6 6 42 8 0.0 6 7 1 43 11 0.0 6 11 44 14 0.0 6 14 45 9 0.0 6 7 2 46 10 0.0 6 8 2 47 10 0.0 6 9 1 48 21 0.0 6 18 2 0 0 0 1 49 18 0.0 6 18 50 13 0.0 6 11 2 51 14 0.0 6 13 1 52 7 0.0 6 4 2 1 53 22 0.0 6 19 3 54 21 0.0 6 21 55 19 0.0 6 16 3 56 27 0.0 6 21 4 1 0 0 0 1 57 10 0.0 6 10 58 23 0.0 6 20 3 59 14 0.0 6 14 60 19 0.0 6 17 2 61 21 0.0 6 19 1 0 0 1 62 26 0.0 6 25 1 63 24 0.0 6 22 2 64 21 0.0 6 19 2 65 20 0.0 6 19 1 66 25 0.0 6 23 2 67 21 0.0 6 19 0 0 0 2 68 21 0.0 6 21 69 49 0.0 6 42 5 0 1 0 0 1 70 120 0.0 6 116 4 71 61 0.0 6 57 2 1 0 0 0 1 72 52 0.0 6 51 0 0 1 73 21 0.0 6 21 74 32 0.0 6 29 3 75 27 0.0 6 26 1 76 26 0.0 6 26 77 27 0.0 6 26 1 78 32 0.0 6 32 79 40 0.0 6 38 2 80 33 0.0 6 33 81 22 0.0 6 20 2 82 59 0.0 6 55 3 1 83 60 0.0 6 60 84 58 0.0 6 58 85 52 0.0 6 51 1 86 51 0.0 6 50 1 87 45 0.0 6 41 4 88 41 0.0 6 40 1 89 52 0.0 6 48 4 90 58 0.0 6 57 1 91 39 0.0 6 39 92 51 0.0 6 46 5 93 59 0.0 6 59 94 57 0.0 6 53 4 95 63 0.0 6 58 4 1 96 75 0.0 6 74 1 97 50 0.0 6 48 1 1 98 72 0.0 6 67 2 0 1 2 99 65 0.0 6 61 2 1 0 0 0 1 100 75 0.0 6 68 4 2 0 0 0 1 101 67 0.0 6 66 1 102 58 0.0 6 57 1 103 83 0.0 6 80 3 104 76 0.0 6 71 3 2 105 84 0.0 6 83 1 106 84 0.0 6 84 107 163 0.0 6 156 4 3 108 76 0.0 6 75 1 109 96 0.0 6 94 2 110 112 0.0 6 110 0 2 111 109 0.0 6 106 2 0 1 112 111 0.0 6 107 4 113 115 0.0 6 113 2 114 129 0.0 6 125 2 0 0 1 1 115 149 0.0 6 144 5 116 116 0.0 6 114 2 117 150 0.0 6 145 3 1 0 0 0 1 118 168 0.0 6 167 1 119 133 0.0 6 131 2 120 144 0.0 6 138 5 0 0 1 121 195 0.0 6 193 2 122 138 0.0 6 135 3 123 121 0.0 6 115 5 0 0 0 0 1 124 150 0.0 6 145 5 125 195 0.0 6 191 2 0 0 1 1 126 202 0.0 6 198 4 127 242 0.0 6 235 5 1 1 128 261 0.0 6 255 4 1 0 1 129 274 0.0 6 266 6 1 0 0 0 1 130 365 0.0 6 357 8 131 339 0.0 6 331 5 0 0 1 2 132 283 0.0 6 278 4 0 0 0 1 133 376 0.0 6 371 4 1 134 356 0.0 6 353 3 135 398 0.0 6 395 2 0 1 136 425 0.0 6 418 6 0 1 137 511 0.0 6 506 4 0 0 0 1 138 594 0.0 6 585 8 0 0 1 139 713 0.0 6 709 4 140 862 0.0 6 857 5 141 829 0.0 6 824 3 2 142 716 0.0 6 709 6 1 143 992 0.0 6 983 6 0 1 1 1 144 850 0.0 6 845 2 2 1 145 1062 0.0 6 1054 5 2 0 0 1 146 1033 0.0 6 1022 10 1 147 1140 0.0 6 1130 10 148 1121 0.0 6 1111 8 1 0 0 0 1 149 1261 0.0 6 1254 6 0 0 0 0 1 150 1055 0.0 6 1049 5 0 0 0 0 1 151 1272 0.0 6 1255 13 1 1 1 0 1 152 1390 0.0 6 1384 5 1 153 1523 0.0 6 1504 15 0 2 1 1 154 1097 0.0 6 1086 10 1 155 1360 0.0 6 1347 12 0 0 0 0 1 156 1530 0.0 6 1519 7 1 2 0 0 1 157 1341 0.0 6 1331 9 0 0 0 0 1 158 1258 0.0 6 1248 9 0 0 0 1 159 1130 0.0 6 1123 6 0 0 1 160 1325 0.0 6 1316 7 0 1 1 161 1263 0.0 6 1250 10 1 2 162 1180 0.0 6 1174 5 0 0 0 1 163 1246 0.0 6 1238 7 0 0 1 164 1156 0.0 6 1149 7 165 1162 0.0 6 1156 5 1 166 1050 0.0 6 1040 7 1 0 0 0 2 167 975 0.0 6 969 4 0 1 0 1 168 860 0.0 6 853 6 0 0 1 169 1409 0.0 6 1400 6 1 1 0 1 170 995 0.0 6 991 4 171 1008 0.0 6 1004 3 1 172 939 0.0 6 929 9 1 173 781 0.0 6 769 12 174 1003 0.0 6 994 8 1 175 867 0.0 6 857 7 0 2 0 0 1 176 902 0.0 6 901 1 177 990 0.0 6 987 2 0 0 0 1 178 832 0.0 6 827 5 179 789 0.0 6 784 3 1 0 1 180 619 0.0 6 615 3 0 0 1 181 587 0.0 6 583 4 182 466 0.0 6 463 3 183 408 0.0 6 407 1 184 426 0.0 6 421 5 185 387 0.0 6 383 4 186 652 0.0 6 649 3 187 751 0.0 6 748 3 188 641 0.0 6 633 5 0 1 0 1 1 189 628 0.0 6 627 1 190 474 0.0 6 470 4 191 445 0.0 6 439 6 192 388 0.0 6 388 193 345 0.0 6 345 194 332 0.0 6 331 1 195 310 0.0 6 310 196 267 0.0 6 265 1 1 197 284 0.0 6 282 2 198 192 0.0 6 192 199 259 0.0 6 257 2 200 196 0.0 6 191 4 1 201 176 0.0 6 172 2 1 0 0 0 1 202 146 0.0 6 145 1 203 153 0.0 6 152 0 1 204 109 0.0 6 108 1 205 119 0.0 6 117 2 206 91 0.0 6 91 207 86 0.0 6 86 208 91 0.0 6 91 209 86 0.0 6 86 210 64 0.0 6 63 1 211 63 0.0 6 63 212 58 0.0 6 58 213 59 0.0 6 59 214 37 0.0 6 37 215 55 0.0 6 54 1 216 132 0.0 6 132 217 58 0.0 6 58 218 35 0.0 6 34 0 0 0 1 219 34 0.0 6 34 220 34 0.0 6 32 2 221 29 0.0 6 27 2 222 31 0.0 6 31 223 17 0.0 6 17 224 31 0.0 6 30 1 225 13 0.0 6 13 226 8 0.0 6 8 227 13 0.0 6 13 228 13 0.0 6 13 229 17 0.0 6 17 230 9 0.0 6 9 231 10 0.0 6 10 232 9 0.0 6 9 233 2 0.0 6 2 234 16 0.0 6 15 1 235 18 0.0 6 18 236 4 0.0 6 4 237 6 0.0 6 6 238 3 0.0 6 3 239 9 0.0 6 9 240 3 0.0 6 3 241 4 0.0 6 4 242 4 0.0 6 4 243 2 0.0 6 2 244 1 0.0 6 1 245 2 0.0 6 2 247 1 0.0 6 1 254 1 0.0 6 1 278 1 0.0 6 1 280 1 0.0 6 1 281 8 0.0 6 8 282 59 0.0 6 59 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1862 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 1 0.0 6 0 1 50 1 0.0 6 1 68 7 0.0 6 7 69 10 0.0 6 10 70 25 0.0 6 25 71 16 0.0 6 16 72 7 0.0 6 6 0 0 0 1 73 26 0.0 6 26 74 18 0.0 6 18 75 23 0.0 6 21 2 76 26 0.0 6 25 0 0 1 77 20 0.0 6 20 78 32 0.0 6 32 79 27 0.0 6 27 80 19 0.0 6 19 81 26 0.0 6 26 82 31 0.0 6 30 0 0 0 1 83 21 0.0 6 21 84 14 0.0 6 14 85 10 0.0 6 10 86 27 0.0 6 27 87 32 0.0 6 30 0 0 0 2 88 13 0.0 6 11 1 0 0 1 89 21 0.0 6 21 90 23 0.0 6 23 91 21 0.0 6 20 0 0 0 1 92 30 0.0 6 30 93 27 0.0 6 27 94 24 0.0 6 24 95 31 0.0 6 30 0 0 0 1 96 17 0.0 6 16 0 0 0 1 97 8 0.0 6 7 0 0 1 98 23 0.0 6 22 0 1 99 46 0.0 6 46 100 29 0.0 6 29 101 20 0.0 6 20 102 10 0.0 6 8 1 0 0 1 103 5 0.0 6 4 0 0 0 1 104 15 0.0 6 15 105 5 0.0 6 5 106 8 0.0 6 8 107 41 0.0 6 40 1 108 21 0.0 6 21 109 23 0.0 6 23 110 3 0.0 6 3 111 4 0.0 6 4 112 11 0.0 6 11 113 15 0.0 6 15 114 7 0.0 6 7 115 6 0.0 6 6 116 23 0.0 6 23 117 13 0.0 6 13 118 14 0.0 6 14 119 42 0.0 6 42 120 10 0.0 6 9 0 0 0 1 121 10 0.0 6 10 122 19 0.0 6 18 0 0 0 1 123 15 0.0 6 15 124 13 0.0 6 12 0 1 125 34 0.0 6 34 126 21 0.0 6 20 0 0 0 1 127 47 0.0 6 46 0 0 0 1 128 23 0.0 6 23 129 19 0.0 6 18 1 130 21 0.0 6 21 131 9 0.0 6 9 132 11 0.0 6 11 133 15 0.0 6 15 134 19 0.0 6 18 1 135 12 0.0 6 11 0 0 0 1 136 16 0.0 6 16 137 16 0.0 6 16 138 21 0.0 6 21 139 8 0.0 6 8 140 15 0.0 6 15 141 15 0.0 6 15 142 6 0.0 6 6 143 11 0.0 6 11 144 1 0.0 6 1 145 14 0.0 6 14 146 36 0.0 6 36 147 9 0.0 6 9 148 3 0.0 6 3 149 7 0.0 6 7 150 10 0.0 6 9 0 0 0 1 151 7 0.0 6 7 152 10 0.0 6 10 153 7 0.0 6 7 154 13 0.0 6 13 155 8 0.0 6 8 156 8 0.0 6 8 157 5 0.0 6 5 158 20 0.0 6 20 159 20 0.0 6 20 160 11 0.0 6 11 161 19 0.0 6 19 162 11 0.0 6 10 0 0 0 1 163 5 0.0 6 5 164 16 0.0 6 16 165 6 0.0 6 6 166 3 0.0 6 3 167 4 0.0 6 4 168 14 0.0 6 14 169 33 0.0 6 33 170 3 0.0 6 3 171 7 0.0 6 7 172 1 0.0 6 1 173 8 0.0 6 8 174 5 0.0 6 5 175 3 0.0 6 3 176 7 0.0 6 7 177 2 0.0 6 1 1 178 12 0.0 6 12 179 1 0.0 6 1 180 9 0.0 6 9 181 5 0.0 6 5 182 4 0.0 6 4 183 2 0.0 6 2 184 3 0.0 6 3 185 3 0.0 6 3 186 5 0.0 6 4 1 187 7 0.0 6 7 188 2 0.0 6 2 189 1 0.0 6 1 190 1 0.0 6 1 191 1 0.0 6 1 193 4 0.0 6 4 195 1 0.0 6 1 196 1 0.0 6 1 197 7 0.0 6 7 198 4 0.0 6 4 199 1 0.0 6 1 201 3 0.0 6 3 202 1 0.0 6 1 203 2 0.0 6 2 204 4 0.0 6 4 205 5 0.0 6 5 210 1 0.0 6 1 211 3 0.0 6 3 213 2 0.0 6 2 214 1 0.0 6 1 215 1 0.0 6 1 221 1 0.0 6 1 224 1 0.0 6 1 225 1 0.0 6 1 231 1 0.0 6 1 249 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_18_57_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_18_284_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_18_57_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_18_284_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.71 s (37 µs/read; 1.63 M reads/minute). === Summary === Total read pairs processed: 46,362 Read 1 with adapter: 46,362 (100.0%) Read 2 with adapter: 46,322 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 40 (0.1%) Pairs written (passing filters): 46,322 (99.9%) Total basepairs processed: 23,478,344 bp Read 1: 13,402,875 bp Read 2: 10,075,469 bp Total written (filtered): 2,918,281 bp (12.4%) Read 1: 1,436,206 bp Read 2: 1,482,075 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 46348 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 181.1 1 0 1 6 1 11.3 1 0 0 1 9 2 0.2 2 1 1 10 1 0.0 3 0 1 15 2 0.0 4 2 20 10 0.0 6 9 1 21 4 0.0 6 4 22 1 0.0 6 1 23 1 0.0 6 1 24 2 0.0 6 2 25 8 0.0 6 8 26 7 0.0 6 7 27 1 0.0 6 1 28 4 0.0 6 4 29 2 0.0 6 2 31 1 0.0 6 1 33 1 0.0 6 1 35 2 0.0 6 2 38 1 0.0 6 1 40 2 0.0 6 2 41 3 0.0 6 3 42 1 0.0 6 1 44 2 0.0 6 2 45 1 0.0 6 1 46 2 0.0 6 2 48 2 0.0 6 0 2 49 1 0.0 6 1 50 2 0.0 6 1 1 52 2 0.0 6 1 1 53 2 0.0 6 2 54 2 0.0 6 2 56 1 0.0 6 1 58 2 0.0 6 2 59 1 0.0 6 1 60 2 0.0 6 2 61 1 0.0 6 1 62 3 0.0 6 3 63 2 0.0 6 2 65 2 0.0 6 0 2 67 4 0.0 6 4 68 1 0.0 6 1 69 3 0.0 6 3 72 3 0.0 6 3 77 3 0.0 6 3 78 2 0.0 6 1 0 0 1 81 2 0.0 6 2 86 7 0.0 6 7 87 2 0.0 6 2 89 2 0.0 6 2 90 2 0.0 6 1 1 91 3 0.0 6 3 92 1 0.0 6 1 93 3 0.0 6 3 95 1 0.0 6 1 98 5 0.0 6 5 100 5 0.0 6 5 101 8 0.0 6 7 1 102 3 0.0 6 3 103 3 0.0 6 2 1 104 1 0.0 6 1 105 2 0.0 6 1 1 106 2 0.0 6 2 107 1 0.0 6 1 108 6 0.0 6 6 109 1 0.0 6 1 110 1 0.0 6 1 111 2 0.0 6 2 112 4 0.0 6 4 113 4 0.0 6 4 114 4 0.0 6 3 1 115 2 0.0 6 2 116 3 0.0 6 2 1 117 6 0.0 6 6 118 2 0.0 6 2 119 4 0.0 6 4 121 7 0.0 6 4 2 1 122 9 0.0 6 6 2 1 123 13 0.0 6 12 1 124 5 0.0 6 5 125 10 0.0 6 9 1 126 5 0.0 6 3 2 128 8 0.0 6 7 1 129 4 0.0 6 3 1 130 7 0.0 6 7 131 5 0.0 6 4 1 132 7 0.0 6 7 133 8 0.0 6 8 134 9 0.0 6 9 135 18 0.0 6 18 136 11 0.0 6 11 137 20 0.0 6 20 138 19 0.0 6 19 139 8 0.0 6 8 140 17 0.0 6 14 3 141 8 0.0 6 8 142 11 0.0 6 11 143 39 0.0 6 38 1 144 18 0.0 6 18 145 14 0.0 6 14 146 7 0.0 6 7 147 27 0.0 6 27 148 32 0.0 6 31 1 149 39 0.0 6 39 150 27 0.0 6 25 1 1 151 14 0.0 6 13 1 152 18 0.0 6 18 153 17 0.0 6 16 1 154 8 0.0 6 8 155 6 0.0 6 6 156 14 0.0 6 12 2 157 30 0.0 6 26 2 2 158 15 0.0 6 15 159 17 0.0 6 16 1 160 23 0.0 6 22 1 161 20 0.0 6 19 1 162 17 0.0 6 16 1 163 23 0.0 6 23 164 29 0.0 6 29 165 17 0.0 6 16 1 166 8 0.0 6 6 2 167 18 0.0 6 17 1 168 12 0.0 6 12 169 22 0.0 6 22 170 21 0.0 6 20 1 171 14 0.0 6 13 1 172 26 0.0 6 26 173 26 0.0 6 23 3 174 28 0.0 6 28 175 63 0.0 6 61 2 176 28 0.0 6 28 177 70 0.0 6 67 3 178 39 0.0 6 36 3 179 40 0.0 6 40 180 45 0.0 6 40 5 181 44 0.0 6 41 3 182 56 0.0 6 54 2 183 44 0.0 6 43 1 184 63 0.0 6 63 185 86 0.0 6 82 4 186 79 0.0 6 78 1 187 60 0.0 6 57 3 188 69 0.0 6 68 1 189 59 0.0 6 55 3 1 190 83 0.0 6 82 0 1 191 72 0.0 6 70 2 192 94 0.0 6 94 193 93 0.0 6 89 4 194 86 0.0 6 85 1 195 108 0.0 6 106 2 196 183 0.0 6 175 8 197 131 0.0 6 129 2 198 197 0.0 6 190 7 199 170 0.0 6 168 2 200 140 0.0 6 137 2 1 201 144 0.0 6 139 5 202 189 0.0 6 185 4 203 174 0.0 6 169 5 204 163 0.0 6 159 4 205 172 0.0 6 165 6 1 206 174 0.0 6 172 2 207 240 0.0 6 232 8 208 204 0.0 6 199 5 209 230 0.0 6 224 6 210 169 0.0 6 165 4 211 88 0.0 6 87 1 212 89 0.0 6 85 4 213 150 0.0 6 146 4 214 168 0.0 6 163 5 215 138 0.0 6 137 1 216 171 0.0 6 168 3 217 185 0.0 6 178 7 218 144 0.0 6 139 4 1 219 142 0.0 6 141 1 220 117 0.0 6 114 3 221 114 0.0 6 111 2 1 222 184 0.0 6 179 5 223 137 0.0 6 129 7 0 0 0 0 1 224 134 0.0 6 129 5 225 190 0.0 6 186 4 226 324 0.0 6 314 10 227 294 0.0 6 292 2 228 246 0.0 6 243 3 229 216 0.0 6 211 5 230 228 0.0 6 225 3 231 184 0.0 6 181 3 232 194 0.0 6 190 3 1 233 214 0.0 6 213 1 234 270 0.0 6 265 5 235 153 0.0 6 151 2 236 99 0.0 6 98 1 237 183 0.0 6 177 6 238 212 0.0 6 210 2 239 226 0.0 6 223 2 0 1 240 285 0.0 6 277 8 241 372 0.0 6 364 7 1 242 372 0.0 6 362 10 243 270 0.0 6 263 6 1 244 236 0.0 6 229 7 245 361 0.0 6 355 6 246 171 0.0 6 170 1 247 168 0.0 6 159 8 0 0 0 0 1 248 125 0.0 6 120 5 249 304 0.0 6 300 3 1 250 214 0.0 6 210 3 1 251 294 0.0 6 290 3 1 252 259 0.0 6 254 4 1 253 172 0.0 6 165 7 254 373 0.0 6 365 7 0 1 255 216 0.0 6 209 7 256 229 0.0 6 227 2 257 257 0.0 6 244 12 1 258 257 0.0 6 252 5 259 359 0.0 6 341 16 2 260 506 0.0 6 493 13 261 228 0.0 6 216 11 0 1 262 280 0.0 6 276 4 263 590 0.0 6 581 9 264 267 0.0 6 259 8 265 267 0.0 6 261 5 1 266 240 0.0 6 228 11 1 267 253 0.0 6 247 5 0 0 0 0 1 268 279 0.0 6 274 5 269 519 0.0 6 506 13 270 282 0.0 6 276 6 271 415 0.0 6 404 11 272 589 0.0 6 580 7 2 273 415 0.0 6 408 7 274 627 0.0 6 610 16 1 275 522 0.0 6 508 14 276 929 0.0 6 902 26 0 1 277 1143 0.0 6 1115 28 278 861 0.0 6 832 27 1 1 279 3398 0.0 6 3335 59 3 0 0 0 1 280 18043 0.0 6 17648 388 6 0 0 0 1 281 86 0.0 6 77 7 2 282 5 0.0 6 4 0 0 1 283 3 0.0 6 3 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 14 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 8 181.1 1 0 8 71 1 0.0 6 1 92 2 0.0 6 2 132 1 0.0 6 0 0 0 0 0 0 1 137 1 0.0 6 0 0 0 0 0 0 1 155 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 46317 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 724.4 0 0 1 5 2 45.3 1 2 7 1 2.8 2 0 1 9 1 0.2 2 1 10 5 0.0 3 5 11 7 0.0 3 7 12 2 0.0 3 1 1 13 5 0.0 3 4 0 0 1 14 6 0.0 4 5 0 1 16 5 0.0 4 5 17 10 0.0 5 5 5 18 6 0.0 5 4 2 19 2 0.0 5 2 20 28 0.0 6 26 2 21 39 0.0 6 38 1 22 23 0.0 6 23 23 14 0.0 6 12 2 24 9 0.0 6 9 25 13 0.0 6 12 1 26 6 0.0 6 6 27 10 0.0 6 7 3 28 12 0.0 6 11 1 29 8 0.0 6 8 30 7 0.0 6 5 2 31 8 0.0 6 7 1 32 2 0.0 6 2 33 3 0.0 6 3 34 10 0.0 6 10 35 8 0.0 6 8 36 4 0.0 6 4 37 9 0.0 6 6 2 0 1 38 4 0.0 6 4 39 6 0.0 6 5 1 40 4 0.0 6 3 1 41 10 0.0 6 9 1 42 5 0.0 6 5 43 10 0.0 6 9 1 44 10 0.0 6 9 1 45 6 0.0 6 4 2 46 11 0.0 6 10 1 47 16 0.0 6 14 2 48 7 0.0 6 5 1 1 49 7 0.0 6 7 50 8 0.0 6 7 1 51 3 0.0 6 3 52 21 0.0 6 18 3 53 10 0.0 6 10 54 20 0.0 6 20 55 11 0.0 6 10 1 56 13 0.0 6 10 3 57 18 0.0 6 14 4 58 12 0.0 6 12 59 8 0.0 6 6 2 60 15 0.0 6 11 4 61 13 0.0 6 13 62 8 0.0 6 8 63 10 0.0 6 8 2 64 13 0.0 6 10 2 1 65 2 0.0 6 2 66 10 0.0 6 9 1 67 92 0.0 6 88 2 0 1 0 1 68 21 0.0 6 19 1 1 69 31 0.0 6 26 5 70 5 0.0 6 3 2 71 20 0.0 6 18 2 72 20 0.0 6 18 2 73 9 0.0 6 8 1 74 4 0.0 6 4 75 6 0.0 6 4 1 1 76 21 0.0 6 20 1 77 11 0.0 6 9 1 0 0 1 78 14 0.0 6 12 2 79 20 0.0 6 16 3 1 80 10 0.0 6 8 2 81 10 0.0 6 9 1 82 24 0.0 6 22 2 83 40 0.0 6 39 1 84 23 0.0 6 22 1 85 21 0.0 6 19 2 86 13 0.0 6 10 2 0 0 1 87 29 0.0 6 27 2 88 15 0.0 6 12 3 89 27 0.0 6 26 1 90 18 0.0 6 16 2 91 19 0.0 6 19 92 18 0.0 6 17 1 93 33 0.0 6 31 2 94 40 0.0 6 39 1 95 26 0.0 6 20 5 1 96 36 0.0 6 34 1 1 97 16 0.0 6 15 1 98 29 0.0 6 28 1 99 28 0.0 6 27 1 100 32 0.0 6 28 4 101 42 0.0 6 34 6 0 0 2 102 41 0.0 6 36 3 1 0 1 103 48 0.0 6 45 3 104 37 0.0 6 35 2 105 34 0.0 6 33 1 106 23 0.0 6 20 3 107 28 0.0 6 28 108 17 0.0 6 16 1 109 19 0.0 6 15 3 0 0 1 110 25 0.0 6 22 3 111 40 0.0 6 36 4 112 27 0.0 6 25 1 1 113 15 0.0 6 14 1 114 47 0.0 6 42 5 115 30 0.0 6 26 3 1 116 41 0.0 6 37 3 1 117 34 0.0 6 31 3 118 41 0.0 6 40 1 119 42 0.0 6 41 1 120 58 0.0 6 55 3 121 51 0.0 6 47 3 1 122 59 0.0 6 55 3 1 123 51 0.0 6 46 5 124 66 0.0 6 62 2 2 125 55 0.0 6 52 3 126 68 0.0 6 64 4 127 60 0.0 6 56 4 128 36 0.0 6 31 5 129 48 0.0 6 43 5 130 42 0.0 6 39 3 131 82 0.0 6 76 6 132 116 0.0 6 110 6 133 91 0.0 6 82 9 134 163 0.0 6 157 6 135 206 0.0 6 200 6 136 70 0.0 6 63 7 137 128 0.0 6 121 6 0 0 1 138 60 0.0 6 57 3 139 121 0.0 6 116 4 0 0 1 140 168 0.0 6 159 7 1 0 1 141 151 0.0 6 144 7 142 191 0.0 6 188 3 143 187 0.0 6 175 11 0 0 1 144 261 0.0 6 249 10 1 0 1 145 124 0.0 6 121 3 146 137 0.0 6 134 3 147 77 0.0 6 73 3 1 148 131 0.0 6 122 9 149 264 0.0 6 250 10 2 1 1 150 432 0.0 6 413 16 2 0 1 151 126 0.0 6 120 6 152 443 0.0 6 423 19 1 153 199 0.0 6 197 2 154 261 0.0 6 247 13 0 0 1 155 214 0.0 6 208 5 1 156 170 0.0 6 164 6 157 303 0.0 6 294 8 1 158 298 0.0 6 290 8 159 135 0.0 6 129 4 0 1 0 1 160 264 0.0 6 258 6 161 162 0.0 6 157 5 162 148 0.0 6 142 6 163 283 0.0 6 273 9 1 164 260 0.0 6 248 10 1 0 1 165 270 0.0 6 260 8 1 0 1 166 335 0.0 6 327 7 1 167 188 0.0 6 181 6 0 0 0 0 1 168 498 0.0 6 485 11 0 2 169 163 0.0 6 153 9 1 170 182 0.0 6 168 14 171 480 0.0 6 466 14 172 185 0.0 6 176 8 0 1 173 274 0.0 6 265 8 1 174 410 0.0 6 393 15 2 175 642 0.0 6 624 14 2 1 1 176 479 0.0 6 463 16 177 450 0.0 6 431 16 3 178 334 0.0 6 327 5 0 0 2 179 410 0.0 6 393 14 1 1 0 1 180 386 0.0 6 368 17 1 181 488 0.0 6 469 18 1 182 116 0.0 6 111 5 183 457 0.0 6 438 19 184 356 0.0 6 349 5 1 1 185 530 0.0 6 512 17 1 186 1090 0.0 6 1058 30 1 0 1 187 940 0.0 6 913 27 188 880 0.0 6 853 22 2 1 2 189 779 0.0 6 747 31 1 190 1274 0.0 6 1231 43 191 1202 0.0 6 1168 33 0 0 1 192 724 0.0 6 705 18 1 193 390 0.0 6 369 20 1 194 870 0.0 6 840 28 2 195 1104 0.0 6 1066 35 0 0 2 1 196 2413 0.0 6 2341 64 7 0 0 0 1 197 626 0.0 6 594 30 2 198 1383 0.0 6 1335 48 199 2356 0.0 6 2298 53 2 0 3 200 745 0.0 6 710 34 1 201 1167 0.0 6 1130 34 2 0 1 202 565 0.0 6 549 14 2 203 516 0.0 6 503 13 204 699 0.0 6 685 14 205 280 0.0 6 273 7 206 252 0.0 6 245 7 207 133 0.0 6 130 3 208 286 0.0 6 278 7 1 209 301 0.0 6 294 5 1 0 1 210 250 0.0 6 243 7 211 224 0.0 6 219 5 212 383 0.0 6 368 14 1 213 167 0.0 6 159 7 0 0 1 214 654 0.0 6 641 12 1 215 387 0.0 6 374 13 216 506 0.0 6 490 14 1 0 1 217 354 0.0 6 343 10 1 218 289 0.0 6 276 13 219 322 0.0 6 313 8 0 1 220 106 0.0 6 102 4 221 330 0.0 6 318 12 222 415 0.0 6 404 11 223 618 0.0 6 605 13 224 244 0.0 6 235 9 225 408 0.0 6 399 8 0 0 1 226 208 0.0 6 204 4 227 249 0.0 6 242 7 228 140 0.0 6 136 4 229 302 0.0 6 295 7 230 162 0.0 6 157 5 231 231 0.0 6 224 7 232 351 0.0 6 335 15 0 1 233 198 0.0 6 190 8 234 113 0.0 6 112 1 235 42 0.0 6 42 236 17 0.0 6 17 237 19 0.0 6 19 238 31 0.0 6 30 1 239 27 0.0 6 26 1 240 86 0.0 6 85 1 241 135 0.0 6 130 5 242 167 0.0 6 162 4 1 243 177 0.0 6 173 4 244 84 0.0 6 82 1 1 245 53 0.0 6 51 2 246 60 0.0 6 59 1 247 20 0.0 6 19 1 248 75 0.0 6 74 1 249 8 0.0 6 8 250 19 0.0 6 18 1 251 29 0.0 6 29 252 7 0.0 6 7 253 4 0.0 6 4 262 1 0.0 6 1 277 1 0.0 6 1 278 30 0.0 6 27 3 279 17 0.0 6 16 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 5 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 85 1 0.0 6 1 89 1 0.0 6 1 91 1 0.0 6 1 93 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_19_58_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_19_285_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_19_58_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_19_285_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.79 s (29 µs/read; 2.05 M reads/minute). === Summary === Total read pairs processed: 61,354 Read 1 with adapter: 61,354 (100.0%) Read 2 with adapter: 61,325 (100.0%) == Read fate breakdown == Pairs that were too short: 2 (0.0%) Pairs discarded as untrimmed: 29 (0.0%) Pairs written (passing filters): 61,323 (99.9%) Total basepairs processed: 29,677,571 bp Read 1: 16,941,939 bp Read 2: 12,735,632 bp Total written (filtered): 3,767,376 bp (12.7%) Read 1: 1,916,915 bp Read 2: 1,850,461 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 61080 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 958.7 0 2 4 2 239.7 1 2 9 1 0.2 2 0 0 0 1 10 1 0.1 3 1 11 1 0.0 3 1 14 1 0.0 4 1 16 2 0.0 4 2 20 24 0.0 6 24 21 14 0.0 6 13 1 22 8 0.0 6 8 23 3 0.0 6 3 24 4 0.0 6 4 25 7 0.0 6 7 26 5 0.0 6 5 27 4 0.0 6 4 28 8 0.0 6 7 1 29 3 0.0 6 2 1 30 2 0.0 6 2 31 1 0.0 6 1 32 2 0.0 6 2 33 2 0.0 6 2 35 1 0.0 6 1 36 1 0.0 6 1 37 2 0.0 6 2 39 2 0.0 6 2 40 4 0.0 6 4 41 2 0.0 6 2 43 2 0.0 6 1 0 1 44 3 0.0 6 3 45 7 0.0 6 7 46 3 0.0 6 2 1 48 1 0.0 6 1 49 2 0.0 6 2 50 3 0.0 6 2 1 51 2 0.0 6 2 52 1 0.0 6 1 53 3 0.0 6 3 54 4 0.0 6 4 57 3 0.0 6 1 2 58 1 0.0 6 1 59 2 0.0 6 2 61 2 0.0 6 2 63 2 0.0 6 2 64 2 0.0 6 2 65 2 0.0 6 1 1 67 3 0.0 6 3 68 7 0.0 6 7 69 3 0.0 6 3 70 1 0.0 6 1 71 4 0.0 6 4 72 2 0.0 6 2 73 1 0.0 6 1 74 2 0.0 6 2 75 2 0.0 6 1 1 76 5 0.0 6 5 77 8 0.0 6 8 78 6 0.0 6 5 1 79 1 0.0 6 1 80 2 0.0 6 2 81 3 0.0 6 3 82 3 0.0 6 3 83 1 0.0 6 1 85 1 0.0 6 1 86 14 0.0 6 14 87 5 0.0 6 5 88 5 0.0 6 5 89 7 0.0 6 7 90 4 0.0 6 4 91 2 0.0 6 2 92 8 0.0 6 7 1 93 3 0.0 6 3 94 4 0.0 6 4 96 2 0.0 6 2 97 5 0.0 6 4 1 98 8 0.0 6 7 1 99 1 0.0 6 1 100 4 0.0 6 4 101 6 0.0 6 6 102 6 0.0 6 6 103 2 0.0 6 1 1 104 5 0.0 6 5 105 10 0.0 6 9 1 106 7 0.0 6 5 1 0 1 107 5 0.0 6 4 1 108 16 0.0 6 15 1 109 3 0.0 6 3 110 2 0.0 6 2 111 7 0.0 6 7 112 12 0.0 6 12 114 7 0.0 6 7 115 8 0.0 6 8 116 9 0.0 6 9 117 9 0.0 6 9 118 3 0.0 6 3 119 10 0.0 6 9 1 120 4 0.0 6 4 121 12 0.0 6 12 122 20 0.0 6 19 1 123 13 0.0 6 13 124 10 0.0 6 9 1 125 14 0.0 6 14 126 7 0.0 6 7 127 8 0.0 6 8 128 12 0.0 6 12 129 16 0.0 6 14 2 130 6 0.0 6 5 1 131 10 0.0 6 10 132 9 0.0 6 9 133 14 0.0 6 14 134 10 0.0 6 10 135 22 0.0 6 22 136 21 0.0 6 21 137 42 0.0 6 41 1 138 42 0.0 6 40 2 139 13 0.0 6 13 140 40 0.0 6 39 1 141 60 0.0 6 60 142 180 0.0 6 178 1 0 1 143 156 0.0 6 156 144 91 0.0 6 88 2 1 145 122 0.0 6 120 2 146 128 0.0 6 127 1 147 141 0.0 6 140 1 148 131 0.0 6 130 1 149 184 0.0 6 181 3 150 150 0.0 6 149 1 151 208 0.0 6 208 152 100 0.0 6 100 153 100 0.0 6 98 2 154 64 0.0 6 64 155 95 0.0 6 95 156 81 0.0 6 78 3 157 80 0.0 6 79 1 158 122 0.0 6 121 1 159 144 0.0 6 144 160 154 0.0 6 152 1 0 1 161 167 0.0 6 166 1 162 113 0.0 6 113 163 141 0.0 6 140 1 164 101 0.0 6 99 2 165 105 0.0 6 103 1 1 166 116 0.0 6 114 2 167 112 0.0 6 112 168 86 0.0 6 86 169 116 0.0 6 115 0 1 170 161 0.0 6 161 171 139 0.0 6 138 1 172 93 0.0 6 91 2 173 129 0.0 6 127 2 174 148 0.0 6 148 175 131 0.0 6 130 1 176 113 0.0 6 109 4 177 125 0.0 6 124 1 178 84 0.0 6 84 179 182 0.0 6 180 1 0 0 0 1 180 116 0.0 6 116 181 99 0.0 6 98 1 182 130 0.0 6 130 183 91 0.0 6 91 184 149 0.0 6 147 2 185 139 0.0 6 134 5 186 203 0.0 6 202 1 187 149 0.0 6 148 1 188 152 0.0 6 151 1 189 118 0.0 6 118 190 137 0.0 6 134 3 191 179 0.0 6 177 2 192 149 0.0 6 147 2 193 192 0.0 6 187 5 194 203 0.0 6 199 4 195 280 0.0 6 278 2 196 239 0.0 6 238 1 197 258 0.0 6 252 6 198 285 0.0 6 280 5 199 247 0.0 6 246 1 200 260 0.0 6 258 2 201 219 0.0 6 215 4 202 222 0.0 6 219 3 203 360 0.0 6 354 6 204 249 0.0 6 240 8 1 205 315 0.0 6 311 4 206 248 0.0 6 243 5 207 408 0.0 6 399 9 208 373 0.0 6 367 6 209 351 0.0 6 341 9 1 210 225 0.0 6 221 3 0 1 211 176 0.0 6 174 2 212 188 0.0 6 186 1 0 1 213 290 0.0 6 280 9 1 214 253 0.0 6 248 4 1 215 185 0.0 6 183 2 216 304 0.0 6 299 5 217 421 0.0 6 413 7 0 0 0 0 1 218 274 0.0 6 266 8 219 305 0.0 6 301 4 220 246 0.0 6 243 3 221 216 0.0 6 212 4 222 326 0.0 6 321 5 223 196 0.0 6 195 1 224 173 0.0 6 171 2 225 272 0.0 6 263 8 1 226 689 0.0 6 680 9 227 597 0.0 6 588 8 1 228 506 0.0 6 497 9 229 341 0.0 6 337 3 0 1 230 393 0.0 6 387 6 231 309 0.0 6 305 4 232 396 0.0 6 388 8 233 470 0.0 6 456 14 234 468 0.0 6 454 14 235 278 0.0 6 273 5 236 187 0.0 6 182 4 1 237 210 0.0 6 206 4 238 241 0.0 6 237 4 239 257 0.0 6 253 3 1 240 346 0.0 6 337 9 241 529 0.0 6 522 7 242 409 0.0 6 397 11 1 243 420 0.0 6 411 8 1 244 311 0.0 6 303 8 245 516 0.0 6 507 9 246 169 0.0 6 166 3 247 261 0.0 6 254 6 1 248 120 0.0 6 117 2 1 249 466 0.0 6 460 6 250 228 0.0 6 222 6 251 364 0.0 6 355 9 252 439 0.0 6 427 11 1 253 256 0.0 6 248 7 0 1 254 633 0.0 6 619 14 255 292 0.0 6 285 6 1 256 263 0.0 6 254 8 1 257 312 0.0 6 303 7 2 258 420 0.0 6 408 11 1 259 559 0.0 6 546 13 260 719 0.0 6 702 17 261 254 0.0 6 241 10 3 262 372 0.0 6 364 7 0 1 263 1143 0.0 6 1122 21 264 416 0.0 6 407 8 1 265 481 0.0 6 473 8 266 296 0.0 6 285 11 267 421 0.0 6 415 6 268 388 0.0 6 379 8 1 269 563 0.0 6 551 12 270 264 0.0 6 253 11 271 517 0.0 6 503 13 1 272 1052 0.0 6 1033 18 1 273 570 0.0 6 559 10 1 274 613 0.0 6 602 11 275 922 0.0 6 900 22 276 1310 0.0 6 1278 30 1 0 0 1 277 1499 0.0 6 1472 26 0 1 278 839 0.0 6 809 29 1 279 1056 0.0 6 1028 25 2 0 1 280 18420 0.0 6 17906 504 8 0 1 1 281 127 0.0 6 109 17 0 1 282 5 0.0 6 4 0 1 283 1 0.0 6 1 291 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 274 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 6 239.7 1 0 6 61 1 0.0 6 1 62 22 0.0 6 22 70 1 0.0 6 1 71 3 0.0 6 3 72 9 0.0 6 9 73 3 0.0 6 3 74 3 0.0 6 3 75 1 0.0 6 1 76 2 0.0 6 2 77 5 0.0 6 5 78 4 0.0 6 4 80 5 0.0 6 5 81 6 0.0 6 6 82 4 0.0 6 4 85 2 0.0 6 2 87 2 0.0 6 2 89 2 0.0 6 2 90 3 0.0 6 3 91 3 0.0 6 3 92 1 0.0 6 1 93 2 0.0 6 2 95 1 0.0 6 1 96 6 0.0 6 6 97 5 0.0 6 5 98 2 0.0 6 2 99 2 0.0 6 2 100 1 0.0 6 1 101 2 0.0 6 2 102 2 0.0 6 2 103 1 0.0 6 1 104 4 0.0 6 4 105 3 0.0 6 3 106 1 0.0 6 1 108 2 0.0 6 2 109 2 0.0 6 2 110 1 0.0 6 1 112 1 0.0 6 1 113 2 0.0 6 2 114 1 0.0 6 1 115 1 0.0 6 1 116 5 0.0 6 5 117 2 0.0 6 2 119 1 0.0 6 1 120 3 0.0 6 3 121 2 0.0 6 2 122 1 0.0 6 1 123 1 0.0 6 1 124 2 0.0 6 2 126 2 0.0 6 2 127 2 0.0 6 2 128 3 0.0 6 3 129 2 0.0 6 2 130 2 0.0 6 2 131 2 0.0 6 2 132 1 0.0 6 1 133 2 0.0 6 2 134 4 0.0 6 4 135 1 0.0 6 0 0 0 0 0 0 1 136 2 0.0 6 2 137 2 0.0 6 2 138 5 0.0 6 5 139 4 0.0 6 4 140 3 0.0 6 3 141 2 0.0 6 2 142 1 0.0 6 1 143 1 0.0 6 1 144 1 0.0 6 1 145 1 0.0 6 1 146 1 0.0 6 1 147 1 0.0 6 1 148 1 0.0 6 1 149 1 0.0 6 1 150 2 0.0 6 2 151 5 0.0 6 5 152 1 0.0 6 1 153 1 0.0 6 1 156 2 0.0 6 2 157 4 0.0 6 4 159 2 0.0 6 2 160 3 0.0 6 3 161 1 0.0 6 1 162 3 0.0 6 3 163 5 0.0 6 5 164 3 0.0 6 3 165 1 0.0 6 1 166 2 0.0 6 2 167 1 0.0 6 1 169 2 0.0 6 2 170 1 0.0 6 1 172 1 0.0 6 1 173 3 0.0 6 3 174 1 0.0 6 1 175 1 0.0 6 1 176 2 0.0 6 2 177 1 0.0 6 1 178 1 0.0 6 1 181 1 0.0 6 1 182 1 0.0 6 1 184 4 0.0 6 4 186 1 0.0 6 1 187 1 0.0 6 1 188 1 0.0 6 1 189 1 0.0 6 1 192 1 0.0 6 1 193 1 0.0 6 1 194 1 0.0 6 1 195 2 0.0 6 2 196 2 0.0 6 2 197 1 0.0 6 1 200 1 0.0 6 1 201 1 0.0 6 1 202 1 0.0 6 1 204 1 0.0 6 1 208 1 0.0 6 1 211 2 0.0 6 2 217 1 0.0 6 1 219 1 0.0 6 1 224 1 0.0 6 1 242 1 0.0 6 1 249 1 0.0 6 1 260 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 61046 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 15.0 1 1 9 1 0.2 2 1 11 1 0.0 3 0 1 13 8 0.0 3 8 14 7 0.0 4 7 16 9 0.0 4 9 17 4 0.0 5 4 18 4 0.0 5 3 1 19 7 0.0 5 5 2 20 37 0.0 6 34 2 1 21 38 0.0 6 36 2 22 25 0.0 6 24 1 23 24 0.0 6 24 24 13 0.0 6 13 25 17 0.0 6 17 26 8 0.0 6 8 27 7 0.0 6 7 28 8 0.0 6 8 29 6 0.0 6 6 30 8 0.0 6 8 31 12 0.0 6 11 1 32 10 0.0 6 9 1 33 1 0.0 6 1 34 9 0.0 6 9 35 4 0.0 6 4 36 5 0.0 6 5 37 11 0.0 6 10 1 38 16 0.0 6 14 2 39 7 0.0 6 6 1 40 11 0.0 6 10 1 41 11 0.0 6 10 1 42 3 0.0 6 3 43 11 0.0 6 10 1 44 16 0.0 6 14 1 0 0 1 45 12 0.0 6 11 0 1 46 13 0.0 6 13 47 16 0.0 6 15 1 48 11 0.0 6 11 49 21 0.0 6 17 3 1 50 14 0.0 6 12 2 51 10 0.0 6 10 52 12 0.0 6 11 1 53 12 0.0 6 9 3 54 14 0.0 6 13 1 55 15 0.0 6 12 2 1 56 12 0.0 6 11 1 57 9 0.0 6 8 1 58 13 0.0 6 11 1 1 59 17 0.0 6 13 4 60 11 0.0 6 10 1 61 5 0.0 6 5 62 18 0.0 6 15 3 63 8 0.0 6 7 0 1 64 13 0.0 6 11 2 65 12 0.0 6 11 1 66 19 0.0 6 18 1 67 14 0.0 6 12 2 68 37 0.0 6 36 1 69 111 0.0 6 109 1 0 0 0 0 1 70 20 0.0 6 18 2 71 56 0.0 6 53 2 0 0 1 72 39 0.0 6 34 5 73 29 0.0 6 26 3 74 13 0.0 6 13 75 12 0.0 6 9 2 1 76 31 0.0 6 27 4 77 23 0.0 6 19 4 78 32 0.0 6 31 1 79 19 0.0 6 17 2 80 24 0.0 6 24 81 17 0.0 6 17 82 27 0.0 6 23 4 83 45 0.0 6 42 3 84 41 0.0 6 39 2 85 37 0.0 6 35 2 86 23 0.0 6 22 1 87 33 0.0 6 30 2 1 88 24 0.0 6 23 1 89 45 0.0 6 38 5 1 1 90 29 0.0 6 27 2 91 40 0.0 6 40 92 24 0.0 6 24 93 37 0.0 6 31 6 94 68 0.0 6 63 5 95 31 0.0 6 30 1 96 36 0.0 6 34 2 97 50 0.0 6 49 1 98 42 0.0 6 40 2 99 37 0.0 6 35 2 100 45 0.0 6 44 1 101 31 0.0 6 30 1 102 76 0.0 6 73 2 0 0 1 103 84 0.0 6 83 0 1 104 71 0.0 6 68 3 105 72 0.0 6 70 2 106 67 0.0 6 62 5 107 69 0.0 6 63 5 0 1 108 56 0.0 6 53 3 109 66 0.0 6 64 1 0 0 1 110 50 0.0 6 49 1 111 41 0.0 6 33 8 112 46 0.0 6 45 1 113 47 0.0 6 44 2 1 114 70 0.0 6 66 3 1 115 65 0.0 6 60 5 116 61 0.0 6 58 3 117 51 0.0 6 49 0 2 118 63 0.0 6 61 2 119 86 0.0 6 84 1 1 120 143 0.0 6 137 5 0 0 1 121 92 0.0 6 86 5 0 0 1 122 111 0.0 6 108 3 123 117 0.0 6 111 5 0 1 124 161 0.0 6 159 2 125 115 0.0 6 111 3 1 126 100 0.0 6 98 2 127 101 0.0 6 99 2 128 78 0.0 6 73 3 1 0 1 129 110 0.0 6 105 5 130 110 0.0 6 109 0 0 0 1 131 154 0.0 6 149 5 132 235 0.0 6 228 7 133 220 0.0 6 214 6 134 252 0.0 6 246 5 0 0 0 1 135 352 0.0 6 344 8 136 203 0.0 6 199 4 137 293 0.0 6 281 11 1 138 216 0.0 6 211 4 1 139 242 0.0 6 233 8 1 140 368 0.0 6 355 11 2 141 323 0.0 6 316 6 1 142 386 0.0 6 376 8 1 0 0 1 143 305 0.0 6 299 3 1 0 1 0 1 144 518 0.0 6 503 15 145 350 0.0 6 344 6 146 392 0.0 6 382 9 1 147 319 0.0 6 312 6 1 148 306 0.0 6 298 6 0 0 2 149 486 0.0 6 480 6 150 561 0.0 6 547 13 0 0 1 151 317 0.0 6 312 4 1 152 776 0.0 6 760 14 2 153 443 0.0 6 431 12 154 588 0.0 6 578 9 1 155 549 0.0 6 533 15 1 156 442 0.0 6 434 8 157 635 0.0 6 615 19 1 158 667 0.0 6 652 15 159 337 0.0 6 330 6 0 1 160 631 0.0 6 619 12 161 416 0.0 6 407 9 162 419 0.0 6 411 7 1 163 529 0.0 6 520 9 164 454 0.0 6 436 15 2 0 1 165 537 0.0 6 522 14 0 1 166 726 0.0 6 707 16 1 0 2 167 309 0.0 6 303 6 168 832 0.0 6 813 19 169 263 0.0 6 252 9 1 0 0 1 170 478 0.0 6 465 12 1 171 816 0.0 6 796 20 172 255 0.0 6 247 6 1 0 0 0 1 173 318 0.0 6 314 4 174 492 0.0 6 475 16 0 0 1 175 914 0.0 6 893 20 0 0 1 176 550 0.0 6 533 17 177 834 0.0 6 819 14 0 1 178 629 0.0 6 602 25 1 0 1 179 642 0.0 6 627 13 1 0 1 180 643 0.0 6 623 19 1 181 739 0.0 6 720 17 2 182 247 0.0 6 242 5 183 555 0.0 6 538 15 0 0 2 184 258 0.0 6 253 3 1 0 1 185 420 0.0 6 402 14 2 2 186 1487 0.0 6 1449 37 0 0 1 187 1219 0.0 6 1174 42 2 0 1 188 1134 0.0 6 1098 32 1 2 1 189 1057 0.0 6 1022 34 1 190 1730 0.0 6 1690 40 191 1329 0.0 6 1289 38 1 1 192 1056 0.0 6 1030 24 1 0 1 193 685 0.0 6 665 17 1 2 194 934 0.0 6 911 21 2 195 1318 0.0 6 1274 43 1 196 2872 0.0 6 2791 79 1 0 1 197 421 0.0 6 397 23 1 198 1835 0.0 6 1771 62 1 0 1 199 3329 0.0 6 3246 78 1 0 3 1 200 590 0.0 6 568 21 0 0 1 201 791 0.0 6 771 19 0 0 1 202 417 0.0 6 410 7 203 452 0.0 6 442 10 204 638 0.0 6 620 17 0 0 1 205 236 0.0 6 230 5 1 206 238 0.0 6 234 4 207 153 0.0 6 148 5 208 292 0.0 6 284 7 0 0 1 209 254 0.0 6 245 9 210 237 0.0 6 229 6 1 0 1 211 383 0.0 6 371 12 212 318 0.0 6 309 9 213 81 0.0 6 77 4 214 220 0.0 6 218 2 215 203 0.0 6 194 8 1 216 618 0.0 6 590 27 1 217 357 0.0 6 349 7 1 218 266 0.0 6 257 9 219 250 0.0 6 244 5 1 220 177 0.0 6 172 5 221 228 0.0 6 214 14 222 367 0.0 6 358 8 0 0 1 223 810 0.0 6 798 12 224 120 0.0 6 113 7 225 274 0.0 6 262 12 226 138 0.0 6 137 1 227 174 0.0 6 170 4 228 95 0.0 6 92 2 1 229 149 0.0 6 144 5 230 203 0.0 6 196 7 231 100 0.0 6 98 2 232 164 0.0 6 161 3 233 216 0.0 6 209 7 234 88 0.0 6 83 5 235 44 0.0 6 42 1 0 0 1 236 44 0.0 6 42 2 237 32 0.0 6 31 1 238 18 0.0 6 18 239 5 0.0 6 5 240 63 0.0 6 60 3 241 34 0.0 6 34 242 99 0.0 6 99 243 150 0.0 6 145 5 244 55 0.0 6 52 3 245 31 0.0 6 30 1 246 15 0.0 6 15 247 8 0.0 6 8 248 12 0.0 6 11 1 249 17 0.0 6 17 250 18 0.0 6 17 1 251 20 0.0 6 20 252 12 0.0 6 12 253 15 0.0 6 13 2 254 3 0.0 6 3 255 3 0.0 6 3 256 1 0.0 6 1 277 1 0.0 6 1 278 1 0.0 6 1 279 10 0.0 6 10 280 32 0.0 6 32 281 211 0.0 6 204 7 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 279 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 64 6 0.0 6 6 70 1 0.0 6 1 71 5 0.0 6 5 72 4 0.0 6 4 73 1 0.0 6 1 74 2 0.0 6 2 75 4 0.0 6 4 76 1 0.0 6 1 77 2 0.0 6 2 78 3 0.0 6 3 79 6 0.0 6 6 80 3 0.0 6 3 81 1 0.0 6 1 82 3 0.0 6 3 83 1 0.0 6 1 84 4 0.0 6 4 85 4 0.0 6 4 86 2 0.0 6 2 87 2 0.0 6 2 88 4 0.0 6 4 89 3 0.0 6 3 90 3 0.0 6 3 92 4 0.0 6 4 93 2 0.0 6 2 94 18 0.0 6 18 95 3 0.0 6 3 96 31 0.0 6 30 1 97 2 0.0 6 2 98 2 0.0 6 2 99 4 0.0 6 4 100 4 0.0 6 4 101 1 0.0 6 1 102 3 0.0 6 3 103 2 0.0 6 2 104 2 0.0 6 2 105 1 0.0 6 1 108 4 0.0 6 4 109 2 0.0 6 2 111 1 0.0 6 1 112 2 0.0 6 2 113 2 0.0 6 2 115 1 0.0 6 1 117 1 0.0 6 1 118 2 0.0 6 2 120 4 0.0 6 4 121 3 0.0 6 3 122 2 0.0 6 2 123 1 0.0 6 1 124 3 0.0 6 3 125 3 0.0 6 3 126 2 0.0 6 2 128 1 0.0 6 1 129 2 0.0 6 2 130 1 0.0 6 1 131 1 0.0 6 1 132 5 0.0 6 5 133 2 0.0 6 2 134 2 0.0 6 2 136 5 0.0 6 5 137 2 0.0 6 2 138 3 0.0 6 3 139 1 0.0 6 1 140 3 0.0 6 3 141 2 0.0 6 2 143 5 0.0 6 5 145 1 0.0 6 1 146 2 0.0 6 2 147 2 0.0 6 2 149 2 0.0 6 2 152 1 0.0 6 1 153 2 0.0 6 2 155 3 0.0 6 3 156 5 0.0 6 5 157 5 0.0 6 5 158 2 0.0 6 2 159 2 0.0 6 2 160 4 0.0 6 4 161 2 0.0 6 2 162 2 0.0 6 2 163 2 0.0 6 2 164 1 0.0 6 1 165 2 0.0 6 2 166 2 0.0 6 2 167 2 0.0 6 2 173 1 0.0 6 1 176 2 0.0 6 2 178 1 0.0 6 1 179 3 0.0 6 3 180 1 0.0 6 1 182 1 0.0 6 1 183 1 0.0 6 1 184 1 0.0 6 1 185 1 0.0 6 1 187 2 0.0 6 2 189 1 0.0 6 1 191 1 0.0 6 1 192 1 0.0 6 1 198 1 0.0 6 1 200 1 0.0 6 0 1 207 1 0.0 6 1 210 1 0.0 6 1 224 1 0.0 6 1 228 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_20_59_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_20_286_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_20_59_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_20_286_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.63 s (24 µs/read; 2.54 M reads/minute). === Summary === Total read pairs processed: 69,038 Read 1 with adapter: 69,038 (100.0%) Read 2 with adapter: 68,997 (99.9%) == Read fate breakdown == Pairs that were too short: 9 (0.0%) Pairs discarded as untrimmed: 41 (0.1%) Pairs written (passing filters): 68,988 (99.9%) Total basepairs processed: 33,237,238 bp Read 1: 18,426,288 bp Read 2: 14,810,950 bp Total written (filtered): 4,393,157 bp (13.2%) Read 1: 2,170,527 bp Read 2: 2,222,630 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 68416 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 1078.7 0 2 2 4 4 269.7 1 2 2 6 2 16.9 1 0 0 2 7 2 4.2 2 1 0 1 9 2 0.3 2 2 14 2 0.0 4 1 1 15 2 0.0 4 2 16 2 0.0 4 1 1 17 3 0.0 5 3 18 1 0.0 5 1 19 1 0.0 5 1 20 59 0.0 6 58 1 21 31 0.0 6 31 22 19 0.0 6 19 23 18 0.0 6 18 24 13 0.0 6 13 25 9 0.0 6 9 26 12 0.0 6 12 27 5 0.0 6 4 0 0 0 0 0 1 28 12 0.0 6 10 2 29 7 0.0 6 6 1 30 3 0.0 6 3 31 1 0.0 6 1 32 5 0.0 6 5 33 2 0.0 6 2 34 4 0.0 6 4 36 1 0.0 6 1 37 2 0.0 6 2 38 6 0.0 6 5 1 39 5 0.0 6 5 40 7 0.0 6 7 41 5 0.0 6 4 1 42 2 0.0 6 2 43 1 0.0 6 1 44 6 0.0 6 6 45 7 0.0 6 7 46 5 0.0 6 5 47 4 0.0 6 4 48 4 0.0 6 4 49 4 0.0 6 4 50 6 0.0 6 6 51 4 0.0 6 4 52 2 0.0 6 2 53 3 0.0 6 3 54 7 0.0 6 7 55 1 0.0 6 1 56 6 0.0 6 6 57 7 0.0 6 6 1 58 2 0.0 6 2 59 6 0.0 6 6 60 4 0.0 6 4 61 1 0.0 6 1 62 7 0.0 6 6 1 63 7 0.0 6 7 64 2 0.0 6 2 65 3 0.0 6 3 66 3 0.0 6 3 67 4 0.0 6 4 68 6 0.0 6 6 69 3 0.0 6 3 70 4 0.0 6 4 71 3 0.0 6 3 72 7 0.0 6 6 1 73 9 0.0 6 9 74 9 0.0 6 8 1 75 2 0.0 6 2 76 3 0.0 6 2 0 0 1 77 5 0.0 6 5 78 7 0.0 6 7 79 5 0.0 6 5 80 1 0.0 6 1 81 9 0.0 6 9 82 4 0.0 6 4 83 6 0.0 6 6 84 2 0.0 6 2 85 3 0.0 6 3 86 14 0.0 6 14 87 8 0.0 6 8 88 3 0.0 6 3 89 12 0.0 6 12 90 5 0.0 6 4 1 91 3 0.0 6 3 92 6 0.0 6 6 93 4 0.0 6 4 94 3 0.0 6 3 95 7 0.0 6 7 96 6 0.0 6 6 97 3 0.0 6 3 98 7 0.0 6 7 99 4 0.0 6 4 100 5 0.0 6 5 101 8 0.0 6 8 102 16 0.0 6 13 2 1 103 14 0.0 6 14 104 9 0.0 6 9 105 7 0.0 6 6 1 106 6 0.0 6 6 107 11 0.0 6 10 1 108 27 0.0 6 24 3 109 7 0.0 6 6 1 110 7 0.0 6 7 111 11 0.0 6 11 112 10 0.0 6 10 113 8 0.0 6 7 1 114 14 0.0 6 13 1 115 18 0.0 6 18 116 10 0.0 6 7 3 117 16 0.0 6 15 1 118 8 0.0 6 8 119 12 0.0 6 11 1 120 9 0.0 6 8 0 0 1 121 8 0.0 6 8 122 34 0.0 6 32 2 123 22 0.0 6 20 2 124 18 0.0 6 17 1 125 26 0.0 6 26 126 18 0.0 6 17 1 127 59 0.0 6 58 1 128 28 0.0 6 27 1 129 33 0.0 6 33 130 17 0.0 6 17 131 15 0.0 6 13 1 0 1 132 25 0.0 6 24 1 133 19 0.0 6 19 134 39 0.0 6 37 2 135 21 0.0 6 18 2 1 136 26 0.0 6 26 137 38 0.0 6 38 138 24 0.0 6 23 0 1 139 26 0.0 6 25 0 1 140 28 0.0 6 27 1 141 36 0.0 6 35 1 142 32 0.0 6 32 143 124 0.0 6 124 144 138 0.0 6 138 145 158 0.0 6 157 0 0 1 146 156 0.0 6 154 2 147 178 0.0 6 178 148 177 0.0 6 176 1 149 185 0.0 6 183 2 150 183 0.0 6 183 151 187 0.0 6 185 1 0 1 152 153 0.0 6 153 153 256 0.0 6 254 2 154 176 0.0 6 176 155 97 0.0 6 97 156 133 0.0 6 132 1 157 107 0.0 6 107 158 160 0.0 6 159 1 159 159 0.0 6 157 2 160 193 0.0 6 190 3 161 243 0.0 6 242 0 0 0 1 162 144 0.0 6 144 163 119 0.0 6 118 1 164 169 0.0 6 168 1 165 204 0.0 6 202 2 166 204 0.0 6 204 167 228 0.0 6 228 168 109 0.0 6 108 0 1 169 157 0.0 6 154 3 170 194 0.0 6 192 2 171 168 0.0 6 166 2 172 170 0.0 6 169 1 173 125 0.0 6 124 1 174 139 0.0 6 138 1 175 118 0.0 6 116 1 0 0 0 0 1 176 150 0.0 6 148 2 177 203 0.0 6 202 1 178 169 0.0 6 168 1 179 165 0.0 6 164 1 180 193 0.0 6 191 2 181 509 0.0 6 509 182 244 0.0 6 243 1 183 149 0.0 6 148 1 184 126 0.0 6 126 185 140 0.0 6 139 1 186 164 0.0 6 164 187 218 0.0 6 215 3 188 144 0.0 6 141 3 189 114 0.0 6 112 2 190 142 0.0 6 142 191 182 0.0 6 181 1 192 176 0.0 6 173 3 193 233 0.0 6 230 3 194 196 0.0 6 191 5 195 289 0.0 6 278 10 0 0 1 196 314 0.0 6 311 3 197 322 0.0 6 319 3 198 502 0.0 6 494 7 1 199 493 0.0 6 483 10 200 471 0.0 6 463 6 2 201 272 0.0 6 269 3 202 365 0.0 6 360 5 203 357 0.0 6 354 2 0 0 1 204 351 0.0 6 348 3 205 397 0.0 6 395 2 206 423 0.0 6 420 2 1 207 547 0.0 6 539 8 208 379 0.0 6 376 3 209 387 0.0 6 377 9 1 210 420 0.0 6 412 8 211 369 0.0 6 363 5 0 1 212 416 0.0 6 410 6 213 536 0.0 6 525 11 214 591 0.0 6 580 11 215 501 0.0 6 491 9 1 216 542 0.0 6 529 13 217 361 0.0 6 353 8 218 339 0.0 6 333 6 219 337 0.0 6 333 3 0 0 1 220 308 0.0 6 306 2 221 278 0.0 6 275 2 0 1 222 321 0.0 6 315 6 223 388 0.0 6 376 11 1 224 568 0.0 6 560 8 225 320 0.0 6 318 2 226 474 0.0 6 469 4 0 0 0 1 227 360 0.0 6 355 5 228 293 0.0 6 291 2 229 250 0.0 6 245 4 0 1 230 345 0.0 6 332 13 231 702 0.0 6 690 12 232 430 0.0 6 421 8 1 233 376 0.0 6 363 13 234 657 0.0 6 643 13 1 235 407 0.0 6 388 17 0 1 1 236 1030 0.0 6 1009 21 237 355 0.0 6 348 5 2 238 338 0.0 6 334 4 239 566 0.0 6 558 8 240 404 0.0 6 395 7 2 241 928 0.0 6 901 27 242 950 0.0 6 916 34 243 1529 0.0 6 1495 33 1 244 553 0.0 6 538 15 245 919 0.0 6 891 28 246 603 0.0 6 574 28 1 247 1516 0.0 6 1489 27 248 349 0.0 6 337 12 249 313 0.0 6 304 9 250 482 0.0 6 473 9 251 647 0.0 6 631 16 252 390 0.0 6 372 17 1 253 860 0.0 6 841 17 2 254 375 0.0 6 358 17 255 707 0.0 6 693 14 256 258 0.0 6 256 2 257 288 0.0 6 282 5 1 258 256 0.0 6 246 10 259 292 0.0 6 281 10 1 260 734 0.0 6 719 15 261 447 0.0 6 429 15 1 2 262 749 0.0 6 723 26 263 673 0.0 6 657 14 2 264 659 0.0 6 638 19 2 265 466 0.0 6 454 12 266 738 0.0 6 722 16 267 311 0.0 6 302 9 268 382 0.0 6 372 10 269 442 0.0 6 424 18 270 438 0.0 6 422 16 271 424 0.0 6 405 18 1 272 403 0.0 6 395 7 1 273 674 0.0 6 659 13 2 274 1514 0.0 6 1457 55 1 1 275 2204 0.0 6 2147 53 3 0 0 1 276 1937 0.0 6 1877 58 2 277 3757 0.0 6 3695 60 2 278 1052 0.0 6 981 69 2 279 7327 0.0 6 7176 149 2 280 1020 0.0 6 1006 14 281 7 0.0 6 7 282 1 0.0 6 0 0 1 284 2 0.0 6 2 285 2 0.0 6 2 287 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 622 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 7 269.7 1 0 7 62 23 0.0 6 22 1 71 2 0.0 6 2 72 3 0.0 6 3 73 5 0.0 6 5 74 3 0.0 6 3 75 6 0.0 6 6 76 5 0.0 6 5 77 4 0.0 6 4 78 1 0.0 6 1 79 2 0.0 6 2 80 10 0.0 6 10 81 5 0.0 6 5 82 3 0.0 6 3 83 4 0.0 6 4 84 6 0.0 6 6 85 4 0.0 6 4 86 6 0.0 6 6 87 5 0.0 6 5 88 7 0.0 6 7 89 6 0.0 6 6 90 3 0.0 6 3 92 6 0.0 6 6 93 4 0.0 6 4 94 5 0.0 6 5 95 6 0.0 6 6 96 3 0.0 6 3 97 4 0.0 6 4 98 3 0.0 6 3 99 1 0.0 6 1 100 3 0.0 6 3 102 3 0.0 6 3 103 1 0.0 6 1 104 1 0.0 6 1 105 2 0.0 6 2 107 5 0.0 6 5 108 6 0.0 6 6 109 13 0.0 6 12 1 110 2 0.0 6 2 111 4 0.0 6 4 112 2 0.0 6 2 113 2 0.0 6 2 114 2 0.0 6 2 115 6 0.0 6 6 116 4 0.0 6 4 117 3 0.0 6 3 118 2 0.0 6 2 119 5 0.0 6 5 120 1 0.0 6 1 121 2 0.0 6 2 123 4 0.0 6 4 124 7 0.0 6 7 125 2 0.0 6 2 126 5 0.0 6 5 127 6 0.0 6 6 128 3 0.0 6 3 129 4 0.0 6 4 130 5 0.0 6 5 131 5 0.0 6 5 132 3 0.0 6 3 133 6 0.0 6 6 134 5 0.0 6 5 135 7 0.0 6 7 136 3 0.0 6 3 137 4 0.0 6 3 0 0 0 0 0 1 138 6 0.0 6 6 139 6 0.0 6 6 140 3 0.0 6 3 141 7 0.0 6 7 142 5 0.0 6 5 143 7 0.0 6 7 144 2 0.0 6 2 145 1 0.0 6 1 146 1 0.0 6 1 147 1 0.0 6 1 148 4 0.0 6 4 149 2 0.0 6 2 151 3 0.0 6 3 152 5 0.0 6 5 153 8 0.0 6 8 154 3 0.0 6 3 155 4 0.0 6 4 156 5 0.0 6 5 157 2 0.0 6 2 158 4 0.0 6 4 159 2 0.0 6 2 160 10 0.0 6 10 161 4 0.0 6 4 162 5 0.0 6 5 163 11 0.0 6 11 164 6 0.0 6 6 166 13 0.0 6 13 167 6 0.0 6 6 168 4 0.0 6 4 169 6 0.0 6 6 170 4 0.0 6 4 174 4 0.0 6 4 175 4 0.0 6 4 176 3 0.0 6 3 177 6 0.0 6 6 178 4 0.0 6 4 179 3 0.0 6 3 180 3 0.0 6 3 181 3 0.0 6 3 182 8 0.0 6 8 183 2 0.0 6 2 184 1 0.0 6 1 185 3 0.0 6 3 186 3 0.0 6 3 187 6 0.0 6 6 188 7 0.0 6 7 189 1 0.0 6 1 190 4 0.0 6 4 191 2 0.0 6 2 193 2 0.0 6 2 194 3 0.0 6 3 195 4 0.0 6 4 196 2 0.0 6 2 197 1 0.0 6 1 199 4 0.0 6 4 200 4 0.0 6 4 201 1 0.0 6 1 202 4 0.0 6 4 203 7 0.0 6 7 204 1 0.0 6 1 205 5 0.0 6 4 0 0 0 0 0 1 206 1 0.0 6 1 207 2 0.0 6 2 208 1 0.0 6 1 211 3 0.0 6 3 212 3 0.0 6 3 213 4 0.0 6 4 214 4 0.0 6 4 217 1 0.0 6 1 219 1 0.0 6 1 220 2 0.0 6 2 221 2 0.0 6 2 222 3 0.0 6 3 223 5 0.0 6 5 224 3 0.0 6 3 225 1 0.0 6 1 226 1 0.0 6 1 228 3 0.0 6 3 229 1 0.0 6 1 230 4 0.0 6 4 231 3 0.0 6 3 232 2 0.0 6 2 233 2 0.0 6 2 236 1 0.0 6 1 237 1 0.0 6 1 239 1 0.0 6 1 244 2 0.0 6 2 245 2 0.0 6 2 246 1 0.0 6 1 247 1 0.0 6 1 248 2 0.0 6 2 250 1 0.0 6 1 258 2 0.0 6 2 262 1 0.0 6 1 264 1 0.0 6 1 270 1 0.0 6 1 271 1 0.0 6 1 272 1 0.0 6 1 273 1 0.0 6 1 277 2 0.0 6 2 284 1 0.0 6 1 294 1 0.0 6 1 297 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 68471 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1078.7 0 1 1 6 2 16.9 1 2 7 1 4.2 2 0 1 9 1 0.3 2 1 10 2 0.1 3 1 1 11 7 0.0 3 7 12 2 0.0 3 2 13 6 0.0 3 6 14 4 0.0 4 3 1 15 2 0.0 4 2 16 7 0.0 4 6 1 17 12 0.0 5 9 3 18 9 0.0 5 8 1 19 7 0.0 5 4 3 20 63 0.0 6 63 21 39 0.0 6 37 2 22 20 0.0 6 19 0 1 23 14 0.0 6 13 1 24 16 0.0 6 15 1 25 22 0.0 6 22 26 10 0.0 6 10 27 14 0.0 6 13 1 28 13 0.0 6 12 1 29 13 0.0 6 10 3 30 13 0.0 6 12 1 31 11 0.0 6 11 32 11 0.0 6 10 1 33 5 0.0 6 4 1 34 10 0.0 6 9 1 35 10 0.0 6 9 1 36 4 0.0 6 4 37 9 0.0 6 7 2 38 6 0.0 6 6 39 6 0.0 6 5 1 40 3 0.0 6 3 41 9 0.0 6 6 2 0 0 1 42 7 0.0 6 6 1 43 5 0.0 6 4 1 44 12 0.0 6 12 45 9 0.0 6 8 1 46 13 0.0 6 11 1 1 47 24 0.0 6 22 2 48 8 0.0 6 7 0 1 49 8 0.0 6 7 1 50 10 0.0 6 8 2 51 11 0.0 6 9 2 52 17 0.0 6 15 2 53 15 0.0 6 15 54 7 0.0 6 7 55 12 0.0 6 12 56 10 0.0 6 9 1 57 20 0.0 6 19 1 58 14 0.0 6 14 59 9 0.0 6 8 1 60 19 0.0 6 16 3 61 8 0.0 6 8 62 13 0.0 6 12 1 63 18 0.0 6 14 4 64 13 0.0 6 10 2 0 0 0 1 65 18 0.0 6 16 1 0 1 66 31 0.0 6 30 1 67 125 0.0 6 117 8 68 33 0.0 6 31 2 69 59 0.0 6 58 1 70 12 0.0 6 11 0 0 0 1 71 42 0.0 6 41 0 1 72 30 0.0 6 29 0 1 73 24 0.0 6 24 74 13 0.0 6 12 1 75 15 0.0 6 14 1 76 15 0.0 6 15 77 30 0.0 6 28 1 1 78 22 0.0 6 22 79 24 0.0 6 22 2 80 20 0.0 6 18 2 81 23 0.0 6 21 2 82 30 0.0 6 28 1 1 83 55 0.0 6 52 3 84 31 0.0 6 31 85 29 0.0 6 26 1 2 86 15 0.0 6 13 1 1 87 37 0.0 6 34 3 88 25 0.0 6 24 0 1 89 26 0.0 6 24 1 0 0 0 0 1 90 36 0.0 6 31 4 0 1 91 31 0.0 6 29 2 92 44 0.0 6 40 2 1 0 1 93 49 0.0 6 47 2 94 44 0.0 6 42 2 95 45 0.0 6 42 3 96 39 0.0 6 35 4 97 48 0.0 6 43 5 98 34 0.0 6 30 3 1 99 46 0.0 6 43 3 100 57 0.0 6 53 4 101 47 0.0 6 46 1 102 48 0.0 6 45 3 103 63 0.0 6 59 3 0 0 0 1 104 79 0.0 6 77 2 105 56 0.0 6 55 1 106 52 0.0 6 50 1 0 1 107 59 0.0 6 57 1 1 108 64 0.0 6 58 6 109 50 0.0 6 45 4 0 0 0 0 1 110 81 0.0 6 78 3 111 59 0.0 6 56 3 112 70 0.0 6 62 8 113 64 0.0 6 62 1 1 114 68 0.0 6 65 2 1 115 80 0.0 6 79 1 116 89 0.0 6 85 4 117 74 0.0 6 70 3 1 118 98 0.0 6 95 2 1 119 93 0.0 6 89 3 1 120 90 0.0 6 88 2 121 91 0.0 6 86 5 122 106 0.0 6 100 6 123 117 0.0 6 113 4 124 109 0.0 6 107 2 125 133 0.0 6 122 10 1 126 99 0.0 6 96 3 127 147 0.0 6 146 1 128 104 0.0 6 102 2 129 125 0.0 6 124 1 130 149 0.0 6 145 3 1 131 157 0.0 6 154 3 132 235 0.0 6 226 8 1 133 251 0.0 6 244 4 3 134 274 0.0 6 269 5 135 279 0.0 6 270 8 0 0 1 136 328 0.0 6 325 3 137 273 0.0 6 265 8 138 334 0.0 6 322 10 1 0 1 139 373 0.0 6 366 7 140 413 0.0 6 402 6 1 1 2 0 1 141 446 0.0 6 437 8 1 142 468 0.0 6 464 4 143 549 0.0 6 538 10 1 144 449 0.0 6 444 3 2 145 483 0.0 6 475 7 0 0 1 146 427 0.0 6 420 5 1 0 0 1 147 472 0.0 6 465 7 148 591 0.0 6 579 10 1 0 0 0 1 149 754 0.0 6 739 14 1 150 775 0.0 6 764 11 151 671 0.0 6 652 16 1 0 2 152 684 0.0 6 676 8 153 754 0.0 6 731 19 0 0 3 1 154 607 0.0 6 593 12 0 0 2 155 630 0.0 6 625 4 1 156 637 0.0 6 627 9 1 157 764 0.0 6 757 7 158 527 0.0 6 514 11 0 0 1 1 159 608 0.0 6 593 13 0 0 1 0 1 160 572 0.0 6 561 9 1 1 161 517 0.0 6 508 8 0 1 162 550 0.0 6 539 8 0 1 2 163 582 0.0 6 568 13 0 0 1 164 514 0.0 6 506 7 1 165 676 0.0 6 660 13 1 0 0 1 1 166 573 0.0 6 560 11 1 0 1 167 778 0.0 6 759 16 0 1 2 168 547 0.0 6 543 4 169 496 0.0 6 490 6 170 655 0.0 6 648 7 171 396 0.0 6 389 5 1 0 0 0 1 172 548 0.0 6 539 8 0 1 173 772 0.0 6 759 13 174 956 0.0 6 927 26 2 0 1 175 671 0.0 6 654 16 0 0 0 1 176 921 0.0 6 899 20 0 0 1 0 1 177 1227 0.0 6 1193 32 1 0 1 178 666 0.0 6 650 14 1 0 1 179 593 0.0 6 574 19 180 854 0.0 6 839 15 181 255 0.0 6 250 4 0 0 1 182 287 0.0 6 282 5 183 411 0.0 6 404 7 184 618 0.0 6 610 7 0 0 1 185 903 0.0 6 876 27 186 715 0.0 6 702 12 0 0 1 187 520 0.0 6 507 13 188 616 0.0 6 600 16 189 600 0.0 6 580 18 1 1 190 448 0.0 6 434 14 191 566 0.0 6 546 19 0 0 1 192 843 0.0 6 821 22 193 273 0.0 6 267 6 194 482 0.0 6 469 13 195 898 0.0 6 863 34 0 0 1 196 526 0.0 6 511 15 197 206 0.0 6 201 5 198 551 0.0 6 535 14 1 0 1 199 415 0.0 6 403 12 200 730 0.0 6 708 19 2 1 201 293 0.0 6 286 6 0 0 0 0 1 202 396 0.0 6 385 11 203 162 0.0 6 156 6 204 224 0.0 6 219 5 205 267 0.0 6 255 12 206 336 0.0 6 330 6 207 218 0.0 6 211 6 1 208 220 0.0 6 208 10 1 0 1 209 246 0.0 6 236 9 1 210 426 0.0 6 396 28 1 0 1 211 2282 0.0 6 2223 58 0 1 212 315 0.0 6 290 21 3 0 0 1 213 982 0.0 6 946 34 2 214 2791 0.0 6 2717 68 3 0 3 215 719 0.0 6 692 26 0 0 1 216 1586 0.0 6 1547 37 1 0 1 217 477 0.0 6 461 16 218 441 0.0 6 421 20 219 369 0.0 6 359 9 1 220 327 0.0 6 303 23 1 221 918 0.0 6 895 19 2 0 2 222 1065 0.0 6 1040 23 0 2 223 263 0.0 6 252 10 1 224 573 0.0 6 552 19 0 0 1 1 225 434 0.0 6 422 12 226 476 0.0 6 462 13 1 227 300 0.0 6 295 5 228 580 0.0 6 566 13 1 229 927 0.0 6 900 27 230 166 0.0 6 164 2 231 159 0.0 6 157 1 1 232 165 0.0 6 157 6 0 2 233 299 0.0 6 291 8 234 565 0.0 6 547 17 0 0 1 235 554 0.0 6 534 20 236 297 0.0 6 289 8 237 88 0.0 6 81 7 238 397 0.0 6 381 16 239 565 0.0 6 548 17 240 415 0.0 6 401 13 1 241 996 0.0 6 973 23 242 306 0.0 6 297 9 243 237 0.0 6 230 7 244 166 0.0 6 164 2 245 65 0.0 6 64 1 246 57 0.0 6 56 1 247 69 0.0 6 65 4 248 244 0.0 6 239 5 249 80 0.0 6 77 2 1 250 132 0.0 6 126 6 251 95 0.0 6 92 2 0 0 1 252 33 0.0 6 31 2 253 21 0.0 6 18 3 254 13 0.0 6 13 257 1 0.0 6 1 259 2 0.0 6 2 262 1 0.0 6 1 264 1 0.0 6 1 268 1 0.0 6 1 276 1 0.0 6 1 277 2 0.0 6 2 278 9 0.0 6 9 279 28 0.0 6 28 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 526 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 59 1 0.0 6 1 71 2 0.0 6 2 72 3 0.0 6 3 73 8 0.0 6 8 74 2 0.0 6 2 75 4 0.0 6 4 76 6 0.0 6 6 77 2 0.0 6 2 78 7 0.0 6 7 79 4 0.0 6 4 80 5 0.0 6 4 1 81 6 0.0 6 6 82 14 0.0 6 13 0 0 0 1 83 3 0.0 6 3 84 2 0.0 6 2 85 6 0.0 6 6 86 7 0.0 6 7 87 3 0.0 6 3 88 7 0.0 6 7 89 4 0.0 6 4 90 9 0.0 6 9 91 3 0.0 6 3 92 3 0.0 6 3 93 6 0.0 6 5 0 0 0 1 94 9 0.0 6 9 95 5 0.0 6 5 96 3 0.0 6 3 97 2 0.0 6 2 98 3 0.0 6 3 99 6 0.0 6 6 100 2 0.0 6 2 101 2 0.0 6 2 102 2 0.0 6 2 103 1 0.0 6 1 104 2 0.0 6 2 106 3 0.0 6 3 107 5 0.0 6 5 108 3 0.0 6 3 109 6 0.0 6 6 110 16 0.0 6 16 111 9 0.0 6 9 112 2 0.0 6 2 113 3 0.0 6 3 114 4 0.0 6 4 115 4 0.0 6 4 116 4 0.0 6 4 117 3 0.0 6 3 119 7 0.0 6 7 120 1 0.0 6 1 121 1 0.0 6 1 122 1 0.0 6 1 123 1 0.0 6 1 124 1 0.0 6 1 125 1 0.0 6 1 126 2 0.0 6 2 127 4 0.0 6 4 128 7 0.0 6 7 129 5 0.0 6 5 130 2 0.0 6 2 131 1 0.0 6 1 132 8 0.0 6 8 133 5 0.0 6 5 134 10 0.0 6 10 135 10 0.0 6 10 136 9 0.0 6 9 137 3 0.0 6 3 138 2 0.0 6 2 139 7 0.0 6 7 140 7 0.0 6 7 141 4 0.0 6 4 142 7 0.0 6 7 143 1 0.0 6 1 144 9 0.0 6 9 145 2 0.0 6 2 146 3 0.0 6 3 147 1 0.0 6 1 148 1 0.0 6 1 149 1 0.0 6 1 150 1 0.0 6 1 151 2 0.0 6 2 152 2 0.0 6 2 153 6 0.0 6 6 154 4 0.0 6 4 155 3 0.0 6 3 156 10 0.0 6 10 157 4 0.0 6 4 158 2 0.0 6 2 159 2 0.0 6 2 160 5 0.0 6 5 161 5 0.0 6 5 162 10 0.0 6 10 163 4 0.0 6 4 164 14 0.0 6 14 165 14 0.0 6 14 166 7 0.0 6 7 167 6 0.0 6 6 168 9 0.0 6 9 169 4 0.0 6 4 170 7 0.0 6 7 171 7 0.0 6 7 173 1 0.0 6 1 175 3 0.0 6 3 176 2 0.0 6 2 177 3 0.0 6 3 178 5 0.0 6 5 180 4 0.0 6 4 181 3 0.0 6 3 182 1 0.0 6 1 183 9 0.0 6 9 184 2 0.0 6 2 185 2 0.0 6 2 187 2 0.0 6 2 188 1 0.0 6 1 189 1 0.0 6 1 190 1 0.0 6 1 191 2 0.0 6 2 194 3 0.0 6 3 195 1 0.0 6 1 199 1 0.0 6 1 201 1 0.0 6 1 202 1 0.0 6 1 203 1 0.0 6 1 204 2 0.0 6 2 208 1 0.0 6 1 209 1 0.0 6 1 211 1 0.0 6 1 212 1 0.0 6 1 213 1 0.0 6 1 224 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_01_48_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_01_275_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_01_48_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_01_275_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_02_49_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_02_276_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_02_49_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_02_276_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_03_50_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_03_277_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_03_50_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_03_277_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_04_51_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_04_278_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_04_51_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_04_278_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_05_52_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_05_279_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_05_52_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_05_279_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_06_53_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_06_280_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_06_53_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_06_280_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_15_54_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_15_281_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_15_54_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_15_281_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_16_55_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_16_282_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_16_55_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_16_282_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_17_56_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_17_283_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_17_56_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_17_283_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_18_57_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_18_284_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_18_57_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_18_284_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_19_58_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_19_285_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_19_58_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_19_285_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_20_59_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_05-Rev_20_286_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_20_59_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_05-Rev_20_286_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_01_60_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_01_287_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_01_60_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_01_287_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.44 s (27 µs/read; 2.18 M reads/minute). === Summary === Total read pairs processed: 88,803 Read 1 with adapter: 88,798 (100.0%) Read 2 with adapter: 88,761 (100.0%) == Read fate breakdown == Pairs that were too short: 6 (0.0%) Pairs discarded as untrimmed: 47 (0.1%) Pairs written (passing filters): 88,750 (99.9%) Total basepairs processed: 43,783,895 bp Read 1: 24,902,838 bp Read 2: 18,881,057 bp Total written (filtered): 5,342,068 bp (12.2%) Read 1: 2,674,256 bp Read 2: 2,667,812 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 88533 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1387.5 0 0 1 4 7 346.9 1 3 4 5 1 86.7 1 1 6 1 21.7 1 0 0 1 7 4 5.4 2 1 0 3 8 1 1.4 2 1 16 2 0.0 4 1 1 17 1 0.0 5 1 20 37 0.0 6 36 1 21 25 0.0 6 24 1 22 6 0.0 6 5 1 23 8 0.0 6 7 1 24 10 0.0 6 9 1 25 13 0.0 6 13 26 5 0.0 6 5 27 5 0.0 6 5 28 3 0.0 6 3 29 8 0.0 6 6 1 1 31 3 0.0 6 3 32 1 0.0 6 1 33 2 0.0 6 2 34 4 0.0 6 4 35 3 0.0 6 2 1 36 6 0.0 6 6 37 2 0.0 6 2 38 1 0.0 6 1 39 2 0.0 6 2 40 1 0.0 6 1 41 10 0.0 6 9 0 0 0 0 0 1 42 2 0.0 6 2 43 2 0.0 6 2 44 8 0.0 6 6 2 45 3 0.0 6 3 46 4 0.0 6 4 47 1 0.0 6 1 48 2 0.0 6 2 49 5 0.0 6 5 50 2 0.0 6 2 51 4 0.0 6 3 1 52 1 0.0 6 1 53 2 0.0 6 2 54 1 0.0 6 1 55 4 0.0 6 2 2 56 6 0.0 6 6 57 3 0.0 6 2 0 0 1 58 5 0.0 6 4 0 0 0 0 1 59 2 0.0 6 1 0 1 60 2 0.0 6 2 61 4 0.0 6 4 62 5 0.0 6 5 63 3 0.0 6 3 64 3 0.0 6 3 65 5 0.0 6 5 66 2 0.0 6 2 67 2 0.0 6 2 68 4 0.0 6 4 69 3 0.0 6 3 70 1 0.0 6 1 71 1 0.0 6 1 72 1 0.0 6 0 1 73 1 0.0 6 1 74 3 0.0 6 3 75 3 0.0 6 3 77 1 0.0 6 1 78 7 0.0 6 6 0 1 79 5 0.0 6 5 80 1 0.0 6 1 81 2 0.0 6 2 82 2 0.0 6 2 83 3 0.0 6 3 84 5 0.0 6 5 85 7 0.0 6 6 1 86 13 0.0 6 12 1 87 5 0.0 6 5 88 4 0.0 6 4 89 4 0.0 6 4 90 6 0.0 6 5 1 91 6 0.0 6 6 92 10 0.0 6 9 1 93 3 0.0 6 3 95 2 0.0 6 2 97 7 0.0 6 7 98 12 0.0 6 10 2 99 6 0.0 6 5 1 100 9 0.0 6 8 1 101 13 0.0 6 13 102 9 0.0 6 8 1 103 8 0.0 6 8 104 7 0.0 6 7 105 6 0.0 6 6 106 6 0.0 6 6 107 12 0.0 6 12 108 21 0.0 6 20 1 109 9 0.0 6 9 110 7 0.0 6 7 111 10 0.0 6 9 1 112 10 0.0 6 10 113 3 0.0 6 3 114 12 0.0 6 11 1 115 8 0.0 6 8 116 9 0.0 6 9 117 17 0.0 6 17 118 5 0.0 6 4 0 0 0 0 1 119 6 0.0 6 6 120 4 0.0 6 4 121 10 0.0 6 9 0 0 1 122 28 0.0 6 26 2 123 21 0.0 6 18 3 124 13 0.0 6 10 3 125 12 0.0 6 11 1 126 22 0.0 6 22 127 29 0.0 6 26 2 0 1 128 27 0.0 6 26 1 129 18 0.0 6 18 130 22 0.0 6 20 2 131 11 0.0 6 11 132 10 0.0 6 9 1 133 15 0.0 6 14 1 134 33 0.0 6 32 1 135 50 0.0 6 50 136 28 0.0 6 28 137 64 0.0 6 62 2 138 28 0.0 6 27 1 139 31 0.0 6 31 140 36 0.0 6 34 2 141 64 0.0 6 62 1 1 142 143 0.0 6 140 2 1 143 151 0.0 6 151 144 118 0.0 6 118 145 111 0.0 6 110 1 146 148 0.0 6 147 1 147 148 0.0 6 144 4 148 92 0.0 6 91 1 149 118 0.0 6 118 150 96 0.0 6 96 151 120 0.0 6 120 152 104 0.0 6 103 1 153 69 0.0 6 69 154 96 0.0 6 94 1 1 155 92 0.0 6 92 156 78 0.0 6 78 157 83 0.0 6 81 2 158 95 0.0 6 93 2 159 87 0.0 6 86 1 160 77 0.0 6 77 161 110 0.0 6 110 162 111 0.0 6 109 2 163 71 0.0 6 69 2 164 101 0.0 6 99 2 165 87 0.0 6 85 2 166 77 0.0 6 77 167 98 0.0 6 97 1 168 77 0.0 6 74 2 0 0 1 169 112 0.0 6 111 1 170 146 0.0 6 144 2 171 109 0.0 6 105 4 172 173 0.0 6 172 1 173 148 0.0 6 146 2 174 132 0.0 6 131 1 175 110 0.0 6 109 1 176 102 0.0 6 99 3 177 95 0.0 6 93 2 178 102 0.0 6 99 3 179 219 0.0 6 218 0 0 0 1 180 139 0.0 6 138 1 181 164 0.0 6 161 3 182 150 0.0 6 147 3 183 86 0.0 6 82 3 0 0 1 184 114 0.0 6 111 3 185 152 0.0 6 149 2 1 186 210 0.0 6 204 6 187 149 0.0 6 143 5 1 188 288 0.0 6 278 10 189 116 0.0 6 111 5 190 208 0.0 6 203 5 191 288 0.0 6 281 7 192 219 0.0 6 211 8 193 298 0.0 6 294 2 2 194 224 0.0 6 219 5 195 302 0.0 6 293 9 196 324 0.0 6 316 6 2 197 278 0.0 6 269 9 198 412 0.0 6 405 7 199 407 0.0 6 397 10 200 544 0.0 6 530 14 201 421 0.0 6 416 5 202 460 0.0 6 453 7 203 420 0.0 6 403 17 204 412 0.0 6 402 9 0 1 205 426 0.0 6 408 16 1 1 206 481 0.0 6 467 13 1 207 389 0.0 6 377 12 208 408 0.0 6 398 10 209 336 0.0 6 329 7 210 357 0.0 6 345 12 211 294 0.0 6 285 7 0 0 1 0 1 212 269 0.0 6 264 5 213 445 0.0 6 432 13 214 589 0.0 6 576 13 215 413 0.0 6 403 10 216 426 0.0 6 417 8 1 217 378 0.0 6 371 7 218 413 0.0 6 404 9 219 402 0.0 6 395 7 220 328 0.0 6 315 13 221 314 0.0 6 303 8 3 222 588 0.0 6 578 10 223 716 0.0 6 704 11 1 224 524 0.0 6 519 5 225 510 0.0 6 500 10 226 414 0.0 6 407 7 227 507 0.0 6 501 5 1 228 320 0.0 6 309 10 0 0 0 0 1 229 354 0.0 6 344 9 0 0 0 0 1 230 266 0.0 6 263 3 231 269 0.0 6 262 6 1 232 270 0.0 6 264 6 233 287 0.0 6 278 9 234 320 0.0 6 312 8 235 321 0.0 6 313 6 2 236 483 0.0 6 473 9 1 237 622 0.0 6 600 21 1 238 814 0.0 6 799 14 1 239 597 0.0 6 580 15 2 240 588 0.0 6 569 19 241 650 0.0 6 634 16 242 616 0.0 6 604 11 1 243 389 0.0 6 379 9 1 244 395 0.0 6 389 6 245 485 0.0 6 467 18 246 521 0.0 6 509 12 247 529 0.0 6 509 20 248 518 0.0 6 510 8 249 461 0.0 6 442 18 1 250 603 0.0 6 583 19 1 251 564 0.0 6 555 9 252 466 0.0 6 453 13 253 392 0.0 6 380 12 254 354 0.0 6 348 6 255 457 0.0 6 446 11 256 654 0.0 6 635 17 0 0 2 257 553 0.0 6 542 11 258 431 0.0 6 418 12 0 0 0 0 1 259 864 0.0 6 846 17 1 260 803 0.0 6 777 26 261 686 0.0 6 672 14 262 406 0.0 6 399 7 263 335 0.0 6 325 9 1 264 463 0.0 6 441 21 1 265 476 0.0 6 459 16 0 0 0 1 266 400 0.0 6 396 3 1 267 382 0.0 6 374 8 268 1180 0.0 6 1134 45 1 269 887 0.0 6 870 16 0 0 1 270 623 0.0 6 605 18 271 744 0.0 6 722 21 0 0 0 1 272 551 0.0 6 526 22 1 1 0 1 273 1121 0.0 6 1078 42 0 0 0 1 274 1285 0.0 6 1252 32 1 275 927 0.0 6 899 28 276 951 0.0 6 912 36 1 2 277 2099 0.0 6 2033 56 8 2 278 3678 0.0 6 3551 123 3 1 279 8551 0.0 6 8334 211 6 280 19599 0.0 6 19167 418 11 0 2 0 1 281 4872 0.0 6 4774 92 4 1 0 1 282 17 0.0 6 14 3 283 11 0.0 6 10 1 284 3 0.0 6 3 285 1 0.0 6 1 286 1 0.0 6 1 287 1 0.0 6 1 290 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 265 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 7 0.0 6 7 69 1 0.0 6 1 70 4 0.0 6 4 71 5 0.0 6 5 72 2 0.0 6 2 73 2 0.0 6 2 74 4 0.0 6 4 75 6 0.0 6 6 76 2 0.0 6 2 77 3 0.0 6 3 78 4 0.0 6 4 79 1 0.0 6 1 80 1 0.0 6 1 81 3 0.0 6 3 82 3 0.0 6 3 83 1 0.0 6 1 84 1 0.0 6 1 86 3 0.0 6 3 87 1 0.0 6 1 88 3 0.0 6 3 89 2 0.0 6 2 90 3 0.0 6 3 91 1 0.0 6 1 92 2 0.0 6 2 95 2 0.0 6 2 96 2 0.0 6 2 97 1 0.0 6 1 98 1 0.0 6 1 99 2 0.0 6 2 100 1 0.0 6 1 101 2 0.0 6 2 102 3 0.0 6 3 103 1 0.0 6 1 104 1 0.0 6 1 105 2 0.0 6 2 106 2 0.0 6 2 107 1 0.0 6 1 108 3 0.0 6 3 110 1 0.0 6 1 112 3 0.0 6 3 113 2 0.0 6 2 114 1 0.0 6 1 115 1 0.0 6 1 116 1 0.0 6 1 118 1 0.0 6 1 119 1 0.0 6 1 120 1 0.0 6 1 121 2 0.0 6 2 123 1 0.0 6 1 125 1 0.0 6 1 126 1 0.0 6 1 129 1 0.0 6 1 132 1 0.0 6 1 134 1 0.0 6 1 135 1 0.0 6 1 136 2 0.0 6 2 137 1 0.0 6 1 138 2 0.0 6 2 139 1 0.0 6 1 141 1 0.0 6 1 142 3 0.0 6 3 145 1 0.0 6 1 148 1 0.0 6 1 149 1 0.0 6 1 151 1 0.0 6 1 152 3 0.0 6 3 154 3 0.0 6 3 155 1 0.0 6 1 157 1 0.0 6 1 158 3 0.0 6 3 159 3 0.0 6 3 160 1 0.0 6 1 161 1 0.0 6 1 162 1 0.0 6 1 163 1 0.0 6 1 164 1 0.0 6 1 165 2 0.0 6 2 166 1 0.0 6 1 168 3 0.0 6 3 169 2 0.0 6 2 171 1 0.0 6 1 173 5 0.0 6 5 174 1 0.0 6 1 175 1 0.0 6 1 176 1 0.0 6 1 177 1 0.0 6 1 183 3 0.0 6 3 185 1 0.0 6 1 186 1 0.0 6 1 188 2 0.0 6 2 189 3 0.0 6 3 190 4 0.0 6 4 192 2 0.0 6 2 193 1 0.0 6 1 194 1 0.0 6 1 195 1 0.0 6 1 199 1 0.0 6 1 200 1 0.0 6 1 201 1 0.0 6 1 202 1 0.0 6 1 203 1 0.0 6 1 204 1 0.0 6 1 206 1 0.0 6 1 207 2 0.0 6 2 209 3 0.0 6 3 210 2 0.0 6 2 211 2 0.0 6 2 212 2 0.0 6 2 215 2 0.0 6 2 216 2 0.0 6 2 218 1 0.0 6 1 219 2 0.0 6 2 220 2 0.0 6 2 221 2 0.0 6 2 222 2 0.0 6 2 224 3 0.0 6 3 226 2 0.0 6 2 227 1 0.0 6 1 228 1 0.0 6 1 229 1 0.0 6 1 230 3 0.0 6 3 231 1 0.0 6 1 232 3 0.0 6 3 233 2 0.0 6 2 235 2 0.0 6 2 236 2 0.0 6 2 237 2 0.0 6 2 240 2 0.0 6 2 242 1 0.0 6 1 243 1 0.0 6 1 245 2 0.0 6 2 246 2 0.0 6 2 247 1 0.0 6 1 248 1 0.0 6 1 249 1 0.0 6 1 250 2 0.0 6 2 257 1 0.0 6 1 259 2 0.0 6 2 260 1 0.0 6 1 262 1 0.0 6 1 264 1 0.0 6 1 265 1 0.0 6 1 266 2 0.0 6 2 267 1 0.0 6 1 268 3 0.0 6 3 276 2 0.0 6 2 285 1 0.0 6 1 286 1 0.0 6 1 297 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 88566 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 8 1 1.4 2 0 1 11 1 0.0 3 1 12 7 0.0 3 7 13 19 0.0 3 15 3 1 14 12 0.0 4 10 2 15 3 0.0 4 3 16 17 0.0 4 16 1 17 18 0.0 5 17 1 18 11 0.0 5 10 0 0 0 0 1 19 21 0.0 5 20 0 0 1 20 98 0.0 6 95 3 21 91 0.0 6 90 1 22 52 0.0 6 48 4 23 49 0.0 6 48 1 24 21 0.0 6 21 25 17 0.0 6 16 1 26 9 0.0 6 8 1 27 6 0.0 6 5 0 0 0 0 0 1 28 9 0.0 6 9 29 15 0.0 6 14 1 30 13 0.0 6 13 31 13 0.0 6 11 1 1 32 7 0.0 6 7 33 11 0.0 6 9 1 1 34 9 0.0 6 8 1 35 12 0.0 6 11 1 36 7 0.0 6 7 37 13 0.0 6 12 1 38 19 0.0 6 17 1 0 0 0 1 39 15 0.0 6 15 40 10 0.0 6 8 2 41 11 0.0 6 10 1 42 10 0.0 6 9 0 1 43 10 0.0 6 7 3 44 16 0.0 6 15 1 45 12 0.0 6 11 1 46 27 0.0 6 27 47 21 0.0 6 19 2 48 12 0.0 6 11 1 49 19 0.0 6 19 50 11 0.0 6 11 51 12 0.0 6 11 1 52 23 0.0 6 22 1 53 18 0.0 6 18 54 22 0.0 6 20 2 55 27 0.0 6 24 3 56 22 0.0 6 19 2 0 1 57 23 0.0 6 21 2 58 19 0.0 6 17 2 59 17 0.0 6 15 2 60 21 0.0 6 19 0 1 0 1 61 9 0.0 6 9 62 20 0.0 6 18 2 63 10 0.0 6 10 64 14 0.0 6 12 2 65 16 0.0 6 15 1 66 24 0.0 6 20 4 67 30 0.0 6 28 1 1 68 37 0.0 6 35 1 1 69 143 0.0 6 138 4 0 0 1 70 29 0.0 6 27 1 0 1 71 72 0.0 6 67 5 72 56 0.0 6 51 4 0 0 1 73 34 0.0 6 30 2 1 1 74 31 0.0 6 30 1 75 23 0.0 6 21 1 0 0 1 76 46 0.0 6 44 2 77 37 0.0 6 36 1 78 35 0.0 6 34 1 79 30 0.0 6 27 3 80 33 0.0 6 30 2 0 0 0 0 1 81 20 0.0 6 20 82 52 0.0 6 48 4 83 64 0.0 6 59 4 0 0 1 84 51 0.0 6 49 2 85 75 0.0 6 73 1 1 86 31 0.0 6 30 1 87 45 0.0 6 43 0 1 1 88 25 0.0 6 25 89 51 0.0 6 44 5 1 0 1 90 58 0.0 6 52 4 1 0 1 91 44 0.0 6 41 3 92 27 0.0 6 26 1 93 79 0.0 6 74 4 1 94 95 0.0 6 91 2 0 0 1 0 1 95 50 0.0 6 48 1 1 96 59 0.0 6 57 1 0 0 0 0 1 97 57 0.0 6 55 2 98 57 0.0 6 55 2 99 65 0.0 6 62 2 0 0 1 100 53 0.0 6 51 2 101 44 0.0 6 42 2 102 98 0.0 6 92 5 1 103 131 0.0 6 127 3 1 104 90 0.0 6 87 2 1 105 108 0.0 6 101 6 1 106 120 0.0 6 114 6 107 102 0.0 6 97 5 108 57 0.0 6 57 109 83 0.0 6 76 7 110 80 0.0 6 79 1 111 72 0.0 6 65 6 1 112 70 0.0 6 63 6 1 113 72 0.0 6 67 3 1 0 1 114 102 0.0 6 99 3 115 88 0.0 6 86 2 116 96 0.0 6 94 2 117 84 0.0 6 80 4 118 106 0.0 6 100 4 1 0 1 119 136 0.0 6 129 6 0 0 1 120 147 0.0 6 139 6 1 0 1 121 100 0.0 6 94 6 122 101 0.0 6 97 4 123 92 0.0 6 87 5 124 119 0.0 6 117 2 125 108 0.0 6 102 6 126 95 0.0 6 92 3 127 147 0.0 6 142 5 128 103 0.0 6 99 1 1 0 1 0 1 129 123 0.0 6 118 5 130 109 0.0 6 106 3 131 174 0.0 6 166 8 132 198 0.0 6 190 6 1 0 0 1 133 167 0.0 6 163 3 0 0 0 1 134 201 0.0 6 194 6 1 135 389 0.0 6 380 9 136 245 0.0 6 237 8 137 307 0.0 6 302 5 138 205 0.0 6 201 2 0 1 1 139 352 0.0 6 348 3 0 0 1 140 481 0.0 6 467 12 1 0 0 1 141 389 0.0 6 376 10 2 0 1 142 517 0.0 6 499 17 0 0 1 143 480 0.0 6 473 6 1 144 613 0.0 6 601 11 0 1 145 382 0.0 6 371 10 1 146 396 0.0 6 385 9 1 0 1 147 309 0.0 6 302 4 1 0 2 148 406 0.0 6 396 10 149 525 0.0 6 514 10 0 1 150 725 0.0 6 703 20 0 1 0 1 151 439 0.0 6 424 14 1 152 1061 0.0 6 1034 22 2 2 1 153 553 0.0 6 537 14 2 154 766 0.0 6 752 11 2 0 1 155 729 0.0 6 708 19 0 0 2 156 552 0.0 6 535 16 0 0 1 157 725 0.0 6 712 12 1 158 706 0.0 6 685 19 1 0 0 1 159 506 0.0 6 494 11 0 0 1 160 716 0.0 6 701 15 161 512 0.0 6 489 21 1 1 162 508 0.0 6 495 12 1 163 618 0.0 6 605 12 1 164 511 0.0 6 498 11 0 1 0 0 1 165 658 0.0 6 635 22 0 0 0 1 166 665 0.0 6 651 13 1 167 429 0.0 6 418 11 168 747 0.0 6 736 11 169 334 0.0 6 326 7 0 1 170 483 0.0 6 470 12 1 171 616 0.0 6 602 14 172 431 0.0 6 417 14 173 655 0.0 6 637 17 1 174 862 0.0 6 819 42 0 0 0 1 175 1663 0.0 6 1603 53 6 0 1 176 955 0.0 6 925 29 0 0 1 177 862 0.0 6 834 25 2 1 178 749 0.0 6 716 28 3 1 1 179 816 0.0 6 791 23 2 180 775 0.0 6 757 17 1 181 829 0.0 6 810 18 0 0 1 182 432 0.0 6 414 17 0 0 1 183 859 0.0 6 833 24 0 1 0 1 184 221 0.0 6 214 6 0 0 1 185 468 0.0 6 452 14 1 0 0 0 1 186 1799 0.0 6 1742 54 1 0 2 187 1573 0.0 6 1527 44 1 1 188 1724 0.0 6 1671 50 1 0 1 1 189 1364 0.0 6 1320 43 1 190 2356 0.0 6 2277 73 3 2 1 191 1678 0.0 6 1635 42 0 0 1 192 1192 0.0 6 1160 30 1 1 193 942 0.0 6 915 24 1 2 194 1416 0.0 6 1359 51 4 0 1 1 195 2533 0.0 6 2457 70 1 2 2 0 1 196 2224 0.0 6 2167 53 3 1 197 890 0.0 6 856 31 3 198 3229 0.0 6 3114 112 0 1 2 199 3207 0.0 6 3115 86 4 0 2 200 1624 0.0 6 1571 49 4 201 1567 0.0 6 1537 27 1 1 1 202 986 0.0 6 942 44 203 1132 0.0 6 1107 22 1 0 2 204 1302 0.0 6 1274 28 205 438 0.0 6 431 7 206 405 0.0 6 393 10 0 0 2 207 359 0.0 6 343 13 3 208 536 0.0 6 523 13 209 458 0.0 6 438 17 3 210 471 0.0 6 452 19 211 612 0.0 6 589 22 1 212 351 0.0 6 342 8 1 213 216 0.0 6 206 10 214 373 0.0 6 364 9 215 696 0.0 6 674 19 1 0 2 216 1078 0.0 6 1047 25 4 0 2 217 901 0.0 6 875 23 0 1 2 218 792 0.0 6 768 21 1 1 1 219 755 0.0 6 728 25 1 1 220 778 0.0 6 757 20 0 1 221 650 0.0 6 634 16 222 339 0.0 6 331 8 223 741 0.0 6 718 23 224 913 0.0 6 889 23 1 225 1000 0.0 6 968 31 0 0 1 226 731 0.0 6 704 27 227 438 0.0 6 431 7 228 364 0.0 6 350 12 0 1 1 229 485 0.0 6 473 12 230 379 0.0 6 368 11 231 418 0.0 6 404 13 0 1 232 517 0.0 6 497 20 233 535 0.0 6 521 12 0 1 1 234 283 0.0 6 278 5 235 125 0.0 6 120 4 0 0 1 236 100 0.0 6 97 3 237 57 0.0 6 57 238 34 0.0 6 33 1 239 55 0.0 6 52 3 240 220 0.0 6 213 7 241 233 0.0 6 225 8 242 241 0.0 6 233 8 243 323 0.0 6 319 4 244 199 0.0 6 194 5 245 136 0.0 6 135 1 246 99 0.0 6 96 3 247 67 0.0 6 65 1 1 248 91 0.0 6 91 249 63 0.0 6 61 2 250 76 0.0 6 72 4 251 10 0.0 6 9 1 252 10 0.0 6 10 253 26 0.0 6 26 254 8 0.0 6 8 255 4 0.0 6 4 256 1 0.0 6 1 257 3 0.0 6 1 2 258 1 0.0 6 1 278 1 0.0 6 1 279 1 0.0 6 1 280 10 0.0 6 8 2 281 21 0.0 6 20 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 195 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 47 1 0.0 6 0 1 50 1 0.0 6 1 51 2 0.0 6 2 57 1 0.0 6 1 66 1 0.0 6 1 71 4 0.0 6 4 72 2 0.0 6 2 73 4 0.0 6 4 74 3 0.0 6 2 1 75 6 0.0 6 6 76 2 0.0 6 2 77 2 0.0 6 2 78 4 0.0 6 4 79 1 0.0 6 1 80 4 0.0 6 4 81 1 0.0 6 1 82 2 0.0 6 2 83 1 0.0 6 1 89 3 0.0 6 3 90 2 0.0 6 2 91 2 0.0 6 2 92 1 0.0 6 1 93 3 0.0 6 3 94 2 0.0 6 2 96 2 0.0 6 2 98 1 0.0 6 1 99 3 0.0 6 3 102 4 0.0 6 4 103 4 0.0 6 4 105 2 0.0 6 2 106 1 0.0 6 1 107 1 0.0 6 1 108 6 0.0 6 6 109 4 0.0 6 4 110 3 0.0 6 3 111 1 0.0 6 1 112 1 0.0 6 1 114 1 0.0 6 1 115 3 0.0 6 3 119 1 0.0 6 1 121 1 0.0 6 1 122 2 0.0 6 2 123 3 0.0 6 3 124 1 0.0 6 1 126 2 0.0 6 2 127 1 0.0 6 1 128 2 0.0 6 2 130 1 0.0 6 1 131 1 0.0 6 1 132 2 0.0 6 2 136 1 0.0 6 1 137 1 0.0 6 1 138 1 0.0 6 1 139 2 0.0 6 2 141 4 0.0 6 4 145 2 0.0 6 2 146 1 0.0 6 1 147 3 0.0 6 3 148 1 0.0 6 1 150 2 0.0 6 2 151 1 0.0 6 1 152 2 0.0 6 2 153 2 0.0 6 2 156 1 0.0 6 1 157 2 0.0 6 2 159 3 0.0 6 3 160 2 0.0 6 2 161 1 0.0 6 1 162 2 0.0 6 2 163 1 0.0 6 1 164 1 0.0 6 1 165 1 0.0 6 1 166 3 0.0 6 3 167 1 0.0 6 1 168 1 0.0 6 1 169 2 0.0 6 2 170 1 0.0 6 1 172 2 0.0 6 2 173 1 0.0 6 1 174 3 0.0 6 1 2 178 1 0.0 6 1 179 3 0.0 6 3 180 1 0.0 6 1 181 2 0.0 6 2 182 2 0.0 6 2 183 2 0.0 6 2 184 2 0.0 6 2 185 1 0.0 6 1 187 3 0.0 6 3 188 3 0.0 6 3 189 2 0.0 6 2 191 1 0.0 6 1 194 1 0.0 6 1 196 2 0.0 6 2 199 1 0.0 6 1 201 1 0.0 6 1 206 1 0.0 6 1 208 1 0.0 6 1 209 1 0.0 6 1 210 1 0.0 6 1 216 1 0.0 6 1 221 1 0.0 6 1 246 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_02_61_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_02_288_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_02_61_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_02_288_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.55 s (25 µs/read; 2.41 M reads/minute). === Summary === Total read pairs processed: 62,201 Read 1 with adapter: 62,197 (100.0%) Read 2 with adapter: 62,201 (100.0%) == Read fate breakdown == Pairs that were too short: 40 (0.1%) Pairs discarded as untrimmed: 4 (0.0%) Pairs written (passing filters): 62,157 (99.9%) Total basepairs processed: 26,633,053 bp Read 1: 15,096,282 bp Read 2: 11,536,771 bp Total written (filtered): 3,878,588 bp (14.6%) Read 1: 1,929,409 bp Read 2: 1,949,179 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 60660 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 971.9 0 1 5 2 60.7 1 2 6 2 15.2 1 1 0 1 7 2 3.8 2 1 0 1 8 1 0.9 2 1 9 1 0.2 2 0 1 10 2 0.1 3 2 11 1 0.0 3 1 13 1 0.0 3 1 15 1 0.0 4 1 16 2 0.0 4 2 17 2 0.0 5 2 19 1 0.0 5 1 20 97 0.0 6 96 1 21 58 0.0 6 56 2 22 57 0.0 6 55 2 23 44 0.0 6 44 24 28 0.0 6 28 25 17 0.0 6 17 26 16 0.0 6 16 27 12 0.0 6 12 28 13 0.0 6 12 1 29 17 0.0 6 15 2 30 6 0.0 6 5 1 31 8 0.0 6 8 32 10 0.0 6 8 2 33 7 0.0 6 7 34 8 0.0 6 8 35 5 0.0 6 3 2 36 3 0.0 6 3 37 7 0.0 6 5 2 38 5 0.0 6 4 0 0 0 0 0 1 39 6 0.0 6 6 40 8 0.0 6 7 1 41 9 0.0 6 8 1 42 8 0.0 6 8 43 10 0.0 6 10 44 5 0.0 6 5 45 2 0.0 6 1 1 46 5 0.0 6 5 47 7 0.0 6 7 48 6 0.0 6 5 1 49 6 0.0 6 6 50 2 0.0 6 2 51 8 0.0 6 8 52 9 0.0 6 9 53 7 0.0 6 6 1 54 8 0.0 6 7 1 55 7 0.0 6 7 56 7 0.0 6 6 1 57 4 0.0 6 4 58 5 0.0 6 5 59 4 0.0 6 4 60 2 0.0 6 2 61 4 0.0 6 4 62 4 0.0 6 4 63 4 0.0 6 4 64 5 0.0 6 5 65 5 0.0 6 5 66 6 0.0 6 6 67 16 0.0 6 16 68 6 0.0 6 6 69 7 0.0 6 7 70 11 0.0 6 11 71 9 0.0 6 9 72 7 0.0 6 6 1 73 8 0.0 6 8 74 7 0.0 6 7 75 11 0.0 6 9 1 0 1 76 14 0.0 6 13 1 77 11 0.0 6 11 78 13 0.0 6 12 1 79 4 0.0 6 4 80 7 0.0 6 6 1 81 6 0.0 6 6 82 8 0.0 6 7 0 0 0 0 0 1 83 11 0.0 6 9 2 84 9 0.0 6 9 85 10 0.0 6 10 86 16 0.0 6 16 87 14 0.0 6 13 0 1 88 4 0.0 6 4 89 11 0.0 6 11 90 10 0.0 6 9 1 91 13 0.0 6 13 92 6 0.0 6 5 1 93 8 0.0 6 8 94 9 0.0 6 9 95 8 0.0 6 8 96 11 0.0 6 11 97 8 0.0 6 8 98 7 0.0 6 7 99 16 0.0 6 15 1 100 14 0.0 6 12 2 101 15 0.0 6 14 1 102 17 0.0 6 17 103 19 0.0 6 18 1 104 11 0.0 6 11 105 17 0.0 6 17 106 18 0.0 6 18 107 21 0.0 6 20 1 108 13 0.0 6 13 109 14 0.0 6 13 0 0 0 0 1 110 12 0.0 6 11 1 111 21 0.0 6 21 112 19 0.0 6 18 1 113 16 0.0 6 16 114 19 0.0 6 18 0 1 115 18 0.0 6 15 2 0 0 1 116 20 0.0 6 18 2 117 26 0.0 6 22 3 1 118 22 0.0 6 22 119 16 0.0 6 15 0 1 120 25 0.0 6 24 1 121 19 0.0 6 18 1 122 14 0.0 6 14 123 16 0.0 6 16 124 26 0.0 6 25 0 0 1 125 22 0.0 6 20 2 126 22 0.0 6 22 127 33 0.0 6 31 2 128 25 0.0 6 25 129 32 0.0 6 31 1 130 26 0.0 6 26 131 34 0.0 6 33 1 132 34 0.0 6 33 1 133 35 0.0 6 35 134 22 0.0 6 21 1 135 36 0.0 6 35 1 136 34 0.0 6 32 2 137 43 0.0 6 41 2 138 39 0.0 6 38 1 139 36 0.0 6 36 140 52 0.0 6 52 141 67 0.0 6 67 142 131 0.0 6 129 2 143 255 0.0 6 255 144 325 0.0 6 322 3 145 212 0.0 6 210 2 146 186 0.0 6 186 147 321 0.0 6 320 0 0 1 148 297 0.0 6 296 1 149 182 0.0 6 182 150 226 0.0 6 225 1 151 232 0.0 6 232 152 228 0.0 6 226 2 153 170 0.0 6 170 154 141 0.0 6 140 1 155 182 0.0 6 177 4 0 1 156 190 0.0 6 189 1 157 161 0.0 6 158 2 0 0 0 0 1 158 166 0.0 6 165 0 0 0 0 1 159 212 0.0 6 208 4 160 161 0.0 6 161 161 191 0.0 6 190 0 0 0 1 162 211 0.0 6 210 1 163 198 0.0 6 196 2 164 173 0.0 6 171 2 165 226 0.0 6 224 2 166 205 0.0 6 203 1 0 1 167 171 0.0 6 170 1 168 246 0.0 6 245 1 169 210 0.0 6 206 3 1 170 215 0.0 6 209 5 1 171 273 0.0 6 270 1 0 2 172 191 0.0 6 188 3 173 231 0.0 6 228 3 174 273 0.0 6 269 2 1 0 0 1 175 253 0.0 6 251 2 176 201 0.0 6 197 2 1 0 0 0 1 177 294 0.0 6 294 178 286 0.0 6 284 2 179 307 0.0 6 303 3 1 180 619 0.0 6 617 2 181 358 0.0 6 351 6 1 182 243 0.0 6 239 4 183 270 0.0 6 267 3 184 281 0.0 6 278 2 1 185 296 0.0 6 291 5 186 330 0.0 6 327 2 0 1 187 327 0.0 6 324 3 188 318 0.0 6 310 7 0 1 189 291 0.0 6 287 3 0 0 1 190 299 0.0 6 296 3 191 317 0.0 6 314 3 192 358 0.0 6 352 3 0 0 0 2 1 193 380 0.0 6 376 4 194 417 0.0 6 411 4 0 1 0 1 195 475 0.0 6 470 4 1 196 457 0.0 6 447 10 197 562 0.0 6 551 9 1 1 198 731 0.0 6 724 5 0 1 0 0 1 199 667 0.0 6 656 9 0 2 200 698 0.0 6 689 9 201 641 0.0 6 634 7 202 583 0.0 6 579 3 0 0 0 1 203 601 0.0 6 599 2 204 690 0.0 6 684 6 205 625 0.0 6 617 6 0 0 0 1 1 206 564 0.0 6 558 5 0 1 207 725 0.0 6 715 9 0 0 0 0 1 208 861 0.0 6 849 10 1 0 0 1 209 705 0.0 6 697 6 0 0 0 1 1 210 773 0.0 6 768 4 0 0 1 211 575 0.0 6 572 3 212 591 0.0 6 586 3 1 0 1 213 729 0.0 6 716 13 214 777 0.0 6 765 9 1 1 1 215 846 0.0 6 840 4 1 0 0 1 216 946 0.0 6 940 6 217 1042 0.0 6 1034 7 0 0 1 218 801 0.0 6 795 5 1 219 881 0.0 6 872 9 220 883 0.0 6 872 9 1 0 0 1 221 756 0.0 6 745 9 2 222 618 0.0 6 613 3 2 223 629 0.0 6 623 5 0 1 224 666 0.0 6 661 5 225 703 0.0 6 698 3 0 0 0 1 1 226 737 0.0 6 730 6 0 0 1 227 822 0.0 6 817 3 0 0 1 1 228 831 0.0 6 821 6 0 1 1 1 1 229 668 0.0 6 658 9 0 0 0 1 230 693 0.0 6 690 1 1 1 231 637 0.0 6 635 2 232 750 0.0 6 744 6 233 705 0.0 6 702 3 234 709 0.0 6 700 9 235 663 0.0 6 657 6 236 619 0.0 6 613 5 1 237 619 0.0 6 615 3 1 238 531 0.0 6 530 1 239 546 0.0 6 544 2 240 570 0.0 6 567 3 241 493 0.0 6 492 1 242 458 0.0 6 452 4 1 1 243 547 0.0 6 543 4 244 475 0.0 6 471 4 245 440 0.0 6 438 2 246 426 0.0 6 424 2 247 372 0.0 6 364 8 248 367 0.0 6 365 2 249 328 0.0 6 323 3 1 1 250 330 0.0 6 329 1 251 342 0.0 6 340 1 0 1 252 298 0.0 6 297 1 253 318 0.0 6 318 254 287 0.0 6 287 255 297 0.0 6 297 256 268 0.0 6 267 1 257 287 0.0 6 286 1 258 244 0.0 6 242 2 259 277 0.0 6 276 1 260 232 0.0 6 230 2 261 297 0.0 6 294 3 262 250 0.0 6 249 1 263 158 0.0 6 158 264 169 0.0 6 169 265 187 0.0 6 185 2 266 189 0.0 6 186 3 267 156 0.0 6 153 2 0 0 1 268 174 0.0 6 172 2 269 176 0.0 6 176 270 126 0.0 6 126 271 150 0.0 6 148 0 0 0 1 0 1 272 152 0.0 6 152 273 120 0.0 6 120 274 120 0.0 6 120 275 138 0.0 6 137 1 276 174 0.0 6 173 1 277 206 0.0 6 206 278 242 0.0 6 242 279 335 0.0 6 332 3 280 1030 0.0 6 1025 4 0 0 1 281 1718 0.0 6 1705 12 1 282 10 0.0 6 10 283 3 0.0 6 1 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1537 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 40 0.0 6 39 1 69 3 0.0 6 3 70 12 0.0 6 12 71 16 0.0 6 16 72 10 0.0 6 10 73 6 0.0 6 6 74 7 0.0 6 7 75 8 0.0 6 8 76 10 0.0 6 10 77 8 0.0 6 8 78 7 0.0 6 7 79 7 0.0 6 7 80 1 0.0 6 1 81 5 0.0 6 5 82 1 0.0 6 1 83 3 0.0 6 3 84 4 0.0 6 4 85 2 0.0 6 2 86 4 0.0 6 4 87 5 0.0 6 5 88 4 0.0 6 4 89 3 0.0 6 3 90 11 0.0 6 11 91 7 0.0 6 7 92 2 0.0 6 2 93 1 0.0 6 1 94 5 0.0 6 5 95 3 0.0 6 3 96 3 0.0 6 3 97 1 0.0 6 1 98 10 0.0 6 10 99 8 0.0 6 8 100 2 0.0 6 2 101 11 0.0 6 11 102 10 0.0 6 10 103 3 0.0 6 3 104 2 0.0 6 2 105 3 0.0 6 3 106 4 0.0 6 4 107 7 0.0 6 7 108 14 0.0 6 14 109 7 0.0 6 7 110 1 0.0 6 1 111 4 0.0 6 4 112 1 0.0 6 1 113 5 0.0 6 5 114 7 0.0 6 7 115 6 0.0 6 6 116 4 0.0 6 4 117 9 0.0 6 9 118 7 0.0 6 7 119 7 0.0 6 7 120 4 0.0 6 4 121 4 0.0 6 4 122 7 0.0 6 7 123 8 0.0 6 8 124 3 0.0 6 3 125 8 0.0 6 8 126 13 0.0 6 13 127 5 0.0 6 5 128 10 0.0 6 10 129 4 0.0 6 4 130 7 0.0 6 7 131 2 0.0 6 2 132 10 0.0 6 10 133 7 0.0 6 7 134 2 0.0 6 2 135 10 0.0 6 10 136 7 0.0 6 7 137 8 0.0 6 8 138 5 0.0 6 5 139 3 0.0 6 3 140 7 0.0 6 7 141 9 0.0 6 9 142 4 0.0 6 4 143 7 0.0 6 7 144 6 0.0 6 6 145 7 0.0 6 7 146 9 0.0 6 9 147 10 0.0 6 10 148 10 0.0 6 10 149 4 0.0 6 4 150 9 0.0 6 9 151 7 0.0 6 7 152 7 0.0 6 7 153 1 0.0 6 1 154 5 0.0 6 5 155 16 0.0 6 16 156 8 0.0 6 8 157 11 0.0 6 11 158 9 0.0 6 9 159 6 0.0 6 6 160 8 0.0 6 8 161 5 0.0 6 5 162 9 0.0 6 9 163 7 0.0 6 7 164 16 0.0 6 16 165 10 0.0 6 10 166 15 0.0 6 15 167 9 0.0 6 9 168 14 0.0 6 14 169 4 0.0 6 4 170 6 0.0 6 6 171 10 0.0 6 10 172 12 0.0 6 12 173 12 0.0 6 12 174 10 0.0 6 10 175 20 0.0 6 20 176 10 0.0 6 10 177 10 0.0 6 10 178 10 0.0 6 9 1 179 4 0.0 6 4 180 11 0.0 6 11 181 13 0.0 6 13 182 10 0.0 6 10 183 12 0.0 6 12 184 9 0.0 6 9 185 14 0.0 6 14 186 12 0.0 6 12 187 13 0.0 6 13 188 11 0.0 6 11 189 13 0.0 6 13 190 13 0.0 6 13 191 10 0.0 6 10 192 11 0.0 6 11 193 19 0.0 6 19 194 11 0.0 6 11 195 13 0.0 6 13 196 10 0.0 6 10 197 12 0.0 6 12 198 9 0.0 6 9 199 9 0.0 6 9 200 11 0.0 6 11 201 15 0.0 6 15 202 5 0.0 6 5 203 12 0.0 6 12 204 12 0.0 6 12 205 13 0.0 6 13 206 9 0.0 6 9 207 9 0.0 6 9 208 7 0.0 6 7 209 9 0.0 6 9 210 16 0.0 6 16 211 10 0.0 6 10 212 7 0.0 6 7 213 7 0.0 6 7 214 5 0.0 6 5 215 5 0.0 6 5 216 14 0.0 6 13 0 0 0 0 0 1 217 15 0.0 6 15 218 7 0.0 6 7 219 13 0.0 6 13 220 6 0.0 6 6 221 7 0.0 6 7 222 12 0.0 6 12 223 9 0.0 6 9 224 12 0.0 6 12 225 6 0.0 6 6 226 7 0.0 6 7 227 12 0.0 6 12 228 11 0.0 6 11 229 9 0.0 6 9 230 9 0.0 6 9 231 3 0.0 6 3 232 7 0.0 6 7 233 5 0.0 6 5 234 8 0.0 6 8 235 3 0.0 6 3 236 8 0.0 6 8 237 4 0.0 6 4 238 2 0.0 6 2 239 7 0.0 6 7 240 9 0.0 6 9 241 4 0.0 6 4 242 3 0.0 6 3 243 7 0.0 6 7 244 5 0.0 6 5 245 6 0.0 6 6 246 8 0.0 6 8 247 8 0.0 6 8 248 6 0.0 6 6 249 1 0.0 6 1 250 3 0.0 6 3 251 3 0.0 6 3 252 5 0.0 6 5 253 6 0.0 6 6 254 1 0.0 6 1 255 4 0.0 6 4 256 3 0.0 6 3 257 2 0.0 6 2 259 8 0.0 6 8 260 3 0.0 6 3 261 1 0.0 6 1 262 3 0.0 6 3 263 4 0.0 6 4 264 3 0.0 6 3 265 5 0.0 6 5 267 1 0.0 6 1 268 1 0.0 6 1 269 1 0.0 6 1 270 4 0.0 6 4 271 1 0.0 6 1 272 2 0.0 6 2 274 2 0.0 6 2 276 3 0.0 6 3 277 1 0.0 6 1 278 1 0.0 6 1 279 1 0.0 6 1 280 1 0.0 6 1 281 1 0.0 6 1 282 2 0.0 6 2 284 2 0.0 6 2 286 1 0.0 6 1 292 1 0.0 6 1 295 1 0.0 6 1 297 1 0.0 6 1 298 2 0.0 6 2 300 1 0.0 6 1 308 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 61082 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 971.9 0 1 4 2 243.0 1 2 5 1 60.7 1 1 7 1 3.8 2 1 8 2 0.9 2 2 10 1 0.1 3 1 11 2 0.0 3 2 12 11 0.0 3 9 2 13 13 0.0 3 11 2 14 21 0.0 4 18 2 1 15 1 0.0 4 1 16 13 0.0 4 13 17 38 0.0 5 36 2 18 18 0.0 5 14 3 1 19 26 0.0 5 21 4 0 1 20 65 0.0 6 62 3 21 40 0.0 6 34 6 22 32 0.0 6 24 8 23 21 0.0 6 19 2 24 26 0.0 6 26 25 19 0.0 6 19 26 26 0.0 6 24 1 1 27 15 0.0 6 15 28 13 0.0 6 10 3 29 17 0.0 6 16 1 30 17 0.0 6 17 31 21 0.0 6 19 1 0 0 1 32 23 0.0 6 21 2 33 13 0.0 6 12 1 34 16 0.0 6 12 4 35 23 0.0 6 21 2 36 13 0.0 6 13 37 14 0.0 6 13 1 38 17 0.0 6 14 2 1 39 9 0.0 6 7 2 40 14 0.0 6 14 41 12 0.0 6 11 1 42 7 0.0 6 6 1 43 17 0.0 6 15 2 44 15 0.0 6 13 1 0 0 1 45 15 0.0 6 11 2 0 0 0 0 2 46 21 0.0 6 20 1 47 19 0.0 6 19 48 19 0.0 6 19 49 21 0.0 6 20 1 50 23 0.0 6 21 1 1 51 25 0.0 6 24 1 52 27 0.0 6 24 3 53 32 0.0 6 28 3 0 0 1 54 31 0.0 6 30 1 55 33 0.0 6 32 0 0 0 0 1 56 21 0.0 6 20 1 57 24 0.0 6 23 1 58 37 0.0 6 30 4 1 0 1 0 1 59 28 0.0 6 24 4 60 31 0.0 6 29 2 61 40 0.0 6 36 3 0 0 1 62 35 0.0 6 31 4 63 36 0.0 6 35 1 64 36 0.0 6 35 1 65 34 0.0 6 33 1 66 30 0.0 6 27 2 0 0 1 67 49 0.0 6 44 3 2 68 139 0.0 6 136 2 0 0 0 1 69 61 0.0 6 56 5 70 82 0.0 6 79 3 71 57 0.0 6 55 2 72 84 0.0 6 73 10 1 73 36 0.0 6 36 74 43 0.0 6 43 75 59 0.0 6 54 5 76 62 0.0 6 60 2 77 34 0.0 6 33 1 78 44 0.0 6 43 1 79 49 0.0 6 49 80 49 0.0 6 46 3 81 68 0.0 6 65 3 82 57 0.0 6 54 3 83 72 0.0 6 68 4 84 83 0.0 6 79 2 1 0 0 1 85 54 0.0 6 53 0 0 0 1 86 58 0.0 6 55 2 1 87 52 0.0 6 49 2 0 0 0 1 88 60 0.0 6 53 6 1 89 56 0.0 6 55 1 90 73 0.0 6 69 4 91 86 0.0 6 82 4 92 90 0.0 6 82 8 93 85 0.0 6 81 4 94 102 0.0 6 99 2 1 95 74 0.0 6 73 0 0 0 0 0 1 96 89 0.0 6 84 5 97 95 0.0 6 92 2 1 98 92 0.0 6 86 5 0 0 1 99 92 0.0 6 88 4 100 65 0.0 6 61 3 0 1 101 107 0.0 6 103 4 102 92 0.0 6 83 7 1 0 0 1 103 96 0.0 6 93 2 1 104 147 0.0 6 145 2 105 191 0.0 6 187 2 0 0 2 106 124 0.0 6 121 3 107 131 0.0 6 126 5 108 129 0.0 6 126 3 109 125 0.0 6 117 7 1 110 152 0.0 6 146 6 111 146 0.0 6 141 5 112 157 0.0 6 150 5 0 1 0 0 1 113 194 0.0 6 190 4 114 162 0.0 6 159 2 0 0 0 0 1 115 149 0.0 6 147 2 116 195 0.0 6 189 5 1 117 167 0.0 6 158 7 0 0 0 1 1 118 219 0.0 6 212 7 119 224 0.0 6 218 5 0 0 0 0 1 120 189 0.0 6 184 3 0 0 0 0 2 121 186 0.0 6 179 5 1 0 0 0 1 122 211 0.0 6 209 2 123 193 0.0 6 188 5 124 242 0.0 6 236 6 125 272 0.0 6 266 5 1 126 249 0.0 6 240 7 0 0 2 127 308 0.0 6 298 8 1 0 0 0 1 128 299 0.0 6 287 12 129 365 0.0 6 355 10 130 395 0.0 6 386 7 0 1 1 131 391 0.0 6 374 10 1 2 3 1 132 402 0.0 6 391 9 1 0 0 1 133 443 0.0 6 433 7 0 1 0 0 2 134 466 0.0 6 452 10 2 0 0 1 1 135 508 0.0 6 490 15 0 2 0 1 136 490 0.0 6 472 16 0 0 1 1 137 486 0.0 6 470 13 1 0 1 1 138 559 0.0 6 537 17 3 1 1 139 674 0.0 6 662 10 0 1 0 1 140 692 0.0 6 676 13 0 2 1 141 645 0.0 6 631 11 1 0 1 0 1 142 714 0.0 6 694 18 1 0 0 0 1 143 805 0.0 6 790 11 0 0 3 0 1 144 804 0.0 6 788 14 0 1 0 1 145 834 0.0 6 817 15 0 2 146 869 0.0 6 849 16 2 1 0 0 1 147 915 0.0 6 887 24 2 1 0 1 148 906 0.0 6 878 22 0 1 2 1 2 149 1033 0.0 6 1016 16 0 0 1 150 1087 0.0 6 1059 22 1 0 0 3 2 151 1455 0.0 6 1409 37 2 1 3 1 2 152 1053 0.0 6 1028 20 2 0 1 1 1 153 1350 0.0 6 1322 22 3 1 1 1 154 1255 0.0 6 1233 18 1 0 0 2 1 155 1058 0.0 6 1031 23 1 1 0 0 2 156 1087 0.0 6 1062 18 0 4 1 1 1 157 971 0.0 6 943 23 3 2 158 1018 0.0 6 993 17 3 2 2 0 1 159 1060 0.0 6 1037 18 2 1 1 0 1 160 977 0.0 6 964 10 1 2 161 883 0.0 6 860 21 1 1 162 888 0.0 6 872 14 0 0 0 2 163 766 0.0 6 753 10 0 1 2 164 841 0.0 6 825 14 1 0 1 165 772 0.0 6 759 12 0 0 0 0 1 166 778 0.0 6 766 11 0 0 0 0 1 167 899 0.0 6 887 9 1 0 0 1 1 168 745 0.0 6 734 9 0 1 0 0 1 169 889 0.0 6 875 11 2 0 1 170 812 0.0 6 799 11 1 1 171 807 0.0 6 797 9 1 172 877 0.0 6 863 11 3 173 833 0.0 6 816 15 1 1 174 838 0.0 6 828 10 175 733 0.0 6 728 5 176 749 0.0 6 737 11 0 1 177 779 0.0 6 766 12 0 0 0 0 1 178 653 0.0 6 646 7 179 630 0.0 6 624 5 0 0 0 1 180 519 0.0 6 516 2 1 181 478 0.0 6 473 4 0 0 0 0 1 182 512 0.0 6 509 3 183 451 0.0 6 447 4 184 666 0.0 6 662 4 185 800 0.0 6 793 6 1 186 677 0.0 6 666 11 187 521 0.0 6 516 4 1 188 458 0.0 6 453 5 189 416 0.0 6 414 2 190 420 0.0 6 416 4 191 376 0.0 6 373 3 192 388 0.0 6 384 3 0 0 0 0 1 193 318 0.0 6 314 4 194 270 0.0 6 267 3 195 247 0.0 6 246 1 196 253 0.0 6 252 1 197 307 0.0 6 298 8 0 1 198 254 0.0 6 251 1 2 199 257 0.0 6 252 4 1 200 216 0.0 6 211 4 0 0 1 201 213 0.0 6 210 2 0 1 202 159 0.0 6 158 1 203 161 0.0 6 160 1 204 170 0.0 6 170 205 140 0.0 6 139 1 206 142 0.0 6 141 1 207 131 0.0 6 130 1 208 124 0.0 6 123 1 209 122 0.0 6 120 2 210 102 0.0 6 101 1 211 83 0.0 6 81 2 212 80 0.0 6 80 213 114 0.0 6 113 1 214 71 0.0 6 69 1 0 1 215 98 0.0 6 97 0 1 216 71 0.0 6 70 1 217 62 0.0 6 62 218 55 0.0 6 54 1 219 63 0.0 6 61 2 220 45 0.0 6 45 221 30 0.0 6 30 222 54 0.0 6 54 223 43 0.0 6 42 1 224 28 0.0 6 27 1 225 22 0.0 6 22 226 24 0.0 6 24 227 25 0.0 6 25 228 19 0.0 6 19 229 22 0.0 6 22 230 8 0.0 6 8 231 4 0.0 6 4 232 12 0.0 6 12 233 8 0.0 6 8 234 7 0.0 6 7 235 6 0.0 6 6 236 4 0.0 6 4 237 5 0.0 6 5 238 5 0.0 6 5 239 2 0.0 6 2 240 3 0.0 6 3 242 2 0.0 6 2 243 2 0.0 6 2 244 1 0.0 6 1 247 1 0.0 6 1 248 1 0.0 6 1 249 1 0.0 6 1 252 1 0.0 6 1 254 1 0.0 6 1 278 2 0.0 6 2 279 11 0.0 6 11 280 95 0.0 6 94 0 0 0 0 0 1 281 2 0.0 6 2 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1119 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 3 98 971.9 0 98 66 1 0.0 6 1 70 7 0.0 6 7 71 2 0.0 6 2 72 14 0.0 6 14 73 13 0.0 6 13 74 5 0.0 6 5 75 10 0.0 6 10 76 13 0.0 6 13 77 17 0.0 6 17 78 5 0.0 6 5 79 9 0.0 6 9 80 7 0.0 6 7 81 28 0.0 6 28 82 4 0.0 6 4 83 6 0.0 6 6 84 2 0.0 6 2 85 7 0.0 6 7 86 2 0.0 6 2 87 6 0.0 6 6 88 7 0.0 6 7 89 1 0.0 6 1 90 4 0.0 6 4 91 5 0.0 6 5 92 4 0.0 6 4 93 7 0.0 6 7 94 4 0.0 6 4 95 8 0.0 6 7 1 96 3 0.0 6 3 97 6 0.0 6 6 98 5 0.0 6 5 99 4 0.0 6 4 100 1 0.0 6 1 101 5 0.0 6 5 102 2 0.0 6 2 103 3 0.0 6 3 104 4 0.0 6 4 105 6 0.0 6 6 106 5 0.0 6 5 107 6 0.0 6 6 108 6 0.0 6 6 109 21 0.0 6 21 110 5 0.0 6 5 111 1 0.0 6 1 112 3 0.0 6 3 113 3 0.0 6 3 114 3 0.0 6 3 115 6 0.0 6 6 116 4 0.0 6 4 117 3 0.0 6 3 118 4 0.0 6 4 119 9 0.0 6 9 120 9 0.0 6 9 121 5 0.0 6 5 122 7 0.0 6 7 123 8 0.0 6 8 124 7 0.0 6 7 125 4 0.0 6 3 0 0 1 126 6 0.0 6 6 127 12 0.0 6 12 128 4 0.0 6 4 130 5 0.0 6 5 131 5 0.0 6 5 132 5 0.0 6 5 133 9 0.0 6 9 134 7 0.0 6 6 1 135 2 0.0 6 2 136 4 0.0 6 4 137 7 0.0 6 7 138 15 0.0 6 15 139 13 0.0 6 12 0 0 0 1 140 13 0.0 6 13 141 4 0.0 6 4 142 11 0.0 6 10 1 143 38 0.0 6 38 144 10 0.0 6 10 145 6 0.0 6 6 146 7 0.0 6 7 147 7 0.0 6 7 148 15 0.0 6 15 149 7 0.0 6 7 150 10 0.0 6 10 151 6 0.0 6 6 152 2 0.0 6 2 153 14 0.0 6 13 0 0 0 1 154 3 0.0 6 3 155 5 0.0 6 5 156 4 0.0 6 4 157 14 0.0 6 14 158 12 0.0 6 12 159 6 0.0 6 6 160 8 0.0 6 8 161 5 0.0 6 5 162 6 0.0 6 6 163 11 0.0 6 11 164 5 0.0 6 5 165 19 0.0 6 19 166 11 0.0 6 11 167 10 0.0 6 10 168 10 0.0 6 10 169 10 0.0 6 10 170 8 0.0 6 8 171 5 0.0 6 5 172 6 0.0 6 6 173 7 0.0 6 7 174 15 0.0 6 15 175 3 0.0 6 3 176 11 0.0 6 11 177 9 0.0 6 9 178 9 0.0 6 9 179 5 0.0 6 5 180 4 0.0 6 4 181 4 0.0 6 4 182 2 0.0 6 2 183 4 0.0 6 4 184 8 0.0 6 8 185 6 0.0 6 6 186 8 0.0 6 8 187 8 0.0 6 8 188 14 0.0 6 14 189 7 0.0 6 7 190 6 0.0 6 6 191 6 0.0 6 6 192 5 0.0 6 5 193 5 0.0 6 5 194 10 0.0 6 10 195 8 0.0 6 8 196 6 0.0 6 6 197 2 0.0 6 2 198 3 0.0 6 3 199 7 0.0 6 7 200 2 0.0 6 2 201 7 0.0 6 7 202 4 0.0 6 4 203 6 0.0 6 6 204 4 0.0 6 4 205 1 0.0 6 1 206 6 0.0 6 6 207 1 0.0 6 1 208 2 0.0 6 2 209 4 0.0 6 4 210 3 0.0 6 3 211 1 0.0 6 1 212 3 0.0 6 3 213 1 0.0 6 1 214 2 0.0 6 2 215 3 0.0 6 3 216 1 0.0 6 1 217 4 0.0 6 4 218 1 0.0 6 1 219 5 0.0 6 5 220 3 0.0 6 3 221 2 0.0 6 2 222 3 0.0 6 3 223 1 0.0 6 1 224 4 0.0 6 4 226 1 0.0 6 1 227 1 0.0 6 1 229 2 0.0 6 2 230 1 0.0 6 1 231 1 0.0 6 1 234 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_03_62_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_03_289_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_03_62_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_03_289_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.66 s (23 µs/read; 2.63 M reads/minute). === Summary === Total read pairs processed: 72,711 Read 1 with adapter: 72,703 (100.0%) Read 2 with adapter: 72,641 (99.9%) == Read fate breakdown == Pairs that were too short: 32 (0.0%) Pairs discarded as untrimmed: 77 (0.1%) Pairs written (passing filters): 72,602 (99.9%) Total basepairs processed: 31,274,749 bp Read 1: 17,762,848 bp Read 2: 13,511,901 bp Total written (filtered): 4,618,507 bp (14.8%) Read 1: 2,264,722 bp Read 2: 2,353,785 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 70820 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1136.1 0 1 4 3 284.0 1 1 2 5 3 71.0 1 1 1 1 6 3 17.8 1 2 0 1 7 4 4.4 2 3 0 1 9 3 0.3 2 3 10 5 0.1 3 5 11 3 0.0 3 3 12 1 0.0 3 1 14 1 0.0 4 0 1 15 4 0.0 4 3 1 16 2 0.0 4 1 1 19 3 0.0 5 2 1 20 151 0.0 6 147 4 21 87 0.0 6 80 7 22 56 0.0 6 55 1 23 59 0.0 6 59 24 35 0.0 6 33 2 25 34 0.0 6 31 3 26 27 0.0 6 27 27 23 0.0 6 22 1 28 10 0.0 6 9 1 29 8 0.0 6 8 30 5 0.0 6 4 0 0 0 0 1 31 11 0.0 6 11 32 5 0.0 6 5 33 3 0.0 6 3 34 12 0.0 6 10 1 0 0 1 35 9 0.0 6 9 36 7 0.0 6 7 37 5 0.0 6 5 38 8 0.0 6 8 39 8 0.0 6 8 40 10 0.0 6 10 41 9 0.0 6 7 2 42 8 0.0 6 8 43 10 0.0 6 9 1 44 10 0.0 6 9 1 45 7 0.0 6 7 46 12 0.0 6 12 47 8 0.0 6 8 48 7 0.0 6 7 49 4 0.0 6 4 50 4 0.0 6 4 51 13 0.0 6 12 1 52 8 0.0 6 8 53 6 0.0 6 6 54 9 0.0 6 9 55 7 0.0 6 7 56 4 0.0 6 3 1 57 3 0.0 6 3 58 10 0.0 6 10 59 4 0.0 6 4 60 10 0.0 6 10 61 5 0.0 6 5 62 2 0.0 6 2 63 6 0.0 6 5 0 1 64 6 0.0 6 6 65 9 0.0 6 9 66 7 0.0 6 7 67 12 0.0 6 12 68 3 0.0 6 3 69 12 0.0 6 12 70 7 0.0 6 7 71 8 0.0 6 8 72 9 0.0 6 8 1 73 10 0.0 6 8 2 74 15 0.0 6 14 1 75 12 0.0 6 12 76 12 0.0 6 10 2 77 15 0.0 6 14 1 78 8 0.0 6 7 1 79 5 0.0 6 5 80 7 0.0 6 7 81 6 0.0 6 6 82 7 0.0 6 5 0 2 83 12 0.0 6 11 1 84 9 0.0 6 9 85 13 0.0 6 12 0 1 86 13 0.0 6 13 87 12 0.0 6 10 1 0 0 0 0 1 88 10 0.0 6 9 1 89 10 0.0 6 10 90 11 0.0 6 11 91 15 0.0 6 15 92 12 0.0 6 11 1 93 6 0.0 6 6 94 11 0.0 6 10 1 95 12 0.0 6 12 96 10 0.0 6 10 97 10 0.0 6 10 98 7 0.0 6 6 0 1 99 9 0.0 6 9 100 7 0.0 6 7 101 16 0.0 6 15 1 102 17 0.0 6 17 103 20 0.0 6 19 1 104 20 0.0 6 19 1 105 21 0.0 6 20 1 106 14 0.0 6 13 1 107 30 0.0 6 28 2 108 12 0.0 6 12 109 14 0.0 6 14 110 12 0.0 6 11 0 1 111 17 0.0 6 16 1 112 19 0.0 6 19 113 20 0.0 6 18 1 1 114 19 0.0 6 18 0 1 115 19 0.0 6 19 116 17 0.0 6 16 1 117 22 0.0 6 21 1 118 16 0.0 6 15 1 119 20 0.0 6 20 120 14 0.0 6 13 1 121 26 0.0 6 24 2 122 16 0.0 6 15 1 123 15 0.0 6 15 124 20 0.0 6 20 125 23 0.0 6 22 1 126 28 0.0 6 28 127 31 0.0 6 26 4 1 128 18 0.0 6 16 2 129 32 0.0 6 31 1 130 38 0.0 6 37 1 131 34 0.0 6 33 1 132 38 0.0 6 37 1 133 20 0.0 6 20 134 28 0.0 6 27 1 135 33 0.0 6 33 136 35 0.0 6 35 137 32 0.0 6 30 2 138 41 0.0 6 38 3 139 35 0.0 6 33 1 0 0 1 140 43 0.0 6 42 1 141 45 0.0 6 45 142 75 0.0 6 75 143 184 0.0 6 183 1 144 243 0.0 6 242 1 145 370 0.0 6 369 0 1 146 230 0.0 6 229 1 147 350 0.0 6 348 2 148 327 0.0 6 323 4 149 286 0.0 6 283 3 150 268 0.0 6 266 2 151 263 0.0 6 262 1 152 242 0.0 6 240 2 153 245 0.0 6 243 2 154 240 0.0 6 237 1 0 1 0 1 155 175 0.0 6 173 1 1 156 190 0.0 6 189 1 157 235 0.0 6 234 0 0 1 158 180 0.0 6 177 3 159 176 0.0 6 176 160 164 0.0 6 164 161 217 0.0 6 215 1 0 0 0 0 1 162 245 0.0 6 245 163 229 0.0 6 226 3 164 240 0.0 6 238 2 165 218 0.0 6 218 166 227 0.0 6 227 167 174 0.0 6 171 2 0 0 1 168 194 0.0 6 193 1 169 330 0.0 6 328 1 1 170 243 0.0 6 241 1 1 171 273 0.0 6 269 4 172 281 0.0 6 281 173 224 0.0 6 222 1 0 0 1 174 268 0.0 6 264 3 0 0 0 1 175 306 0.0 6 304 2 176 279 0.0 6 276 2 0 0 0 1 177 331 0.0 6 328 2 1 178 322 0.0 6 319 2 0 0 0 0 1 179 223 0.0 6 222 1 180 285 0.0 6 283 2 181 946 0.0 6 943 3 182 463 0.0 6 458 5 183 272 0.0 6 271 1 184 334 0.0 6 329 3 0 1 0 1 185 322 0.0 6 320 2 186 346 0.0 6 345 1 187 329 0.0 6 326 3 188 326 0.0 6 320 2 1 0 0 1 2 189 284 0.0 6 283 1 190 291 0.0 6 289 2 191 287 0.0 6 283 4 192 351 0.0 6 346 4 0 0 0 1 193 365 0.0 6 358 7 194 416 0.0 6 408 6 0 1 1 195 480 0.0 6 477 3 196 554 0.0 6 551 3 197 675 0.0 6 671 3 1 198 731 0.0 6 726 5 199 850 0.0 6 844 5 0 1 200 850 0.0 6 843 7 201 682 0.0 6 676 5 1 202 679 0.0 6 668 7 0 2 1 1 203 717 0.0 6 704 12 0 1 204 704 0.0 6 698 4 0 0 0 0 2 205 859 0.0 6 853 5 0 0 0 1 206 677 0.0 6 668 9 207 736 0.0 6 728 7 0 1 208 1016 0.0 6 1001 14 1 209 705 0.0 6 697 8 210 924 0.0 6 920 4 211 797 0.0 6 791 5 1 212 754 0.0 6 746 8 213 887 0.0 6 883 3 1 214 828 0.0 6 816 8 1 0 0 1 2 215 914 0.0 6 903 10 0 0 1 216 1059 0.0 6 1052 6 0 0 1 217 1218 0.0 6 1208 7 1 0 0 2 218 890 0.0 6 882 7 0 0 1 219 934 0.0 6 926 6 0 0 0 1 1 220 960 0.0 6 954 6 221 878 0.0 6 869 8 1 222 763 0.0 6 753 9 0 0 0 1 223 722 0.0 6 716 5 0 0 0 0 1 224 731 0.0 6 722 8 1 225 820 0.0 6 812 7 0 0 0 1 226 808 0.0 6 801 5 1 0 0 1 227 868 0.0 6 859 8 0 0 0 0 1 228 939 0.0 6 926 12 0 1 229 809 0.0 6 803 4 0 0 0 2 230 734 0.0 6 729 3 0 1 0 0 1 231 764 0.0 6 761 1 1 0 0 0 1 232 842 0.0 6 834 7 0 0 0 1 233 771 0.0 6 767 4 234 733 0.0 6 732 1 235 700 0.0 6 695 4 0 0 0 1 236 665 0.0 6 659 4 0 0 0 1 1 237 636 0.0 6 633 3 238 757 0.0 6 753 2 1 0 0 0 1 239 650 0.0 6 647 3 240 671 0.0 6 666 4 0 0 1 241 694 0.0 6 687 7 242 716 0.0 6 712 3 0 0 0 0 1 243 725 0.0 6 719 5 1 244 688 0.0 6 685 3 245 657 0.0 6 657 246 546 0.0 6 541 4 0 1 247 497 0.0 6 492 3 2 248 408 0.0 6 404 4 249 424 0.0 6 423 1 250 459 0.0 6 457 2 251 418 0.0 6 417 1 252 430 0.0 6 428 1 1 253 376 0.0 6 373 2 0 0 0 1 254 388 0.0 6 383 4 0 1 255 378 0.0 6 377 1 256 348 0.0 6 344 4 257 316 0.0 6 313 3 258 313 0.0 6 309 4 259 336 0.0 6 335 1 260 324 0.0 6 321 2 1 261 344 0.0 6 343 1 262 346 0.0 6 344 2 263 234 0.0 6 234 264 248 0.0 6 246 0 0 1 0 0 1 265 268 0.0 6 264 3 1 266 197 0.0 6 197 267 201 0.0 6 199 2 268 225 0.0 6 224 1 269 231 0.0 6 228 2 1 270 230 0.0 6 229 1 271 248 0.0 6 247 1 272 184 0.0 6 183 0 1 273 177 0.0 6 174 2 0 0 0 0 1 274 178 0.0 6 175 1 1 1 275 227 0.0 6 226 1 276 207 0.0 6 205 1 0 0 1 277 262 0.0 6 258 3 1 278 369 0.0 6 367 2 279 433 0.0 6 431 2 280 1383 0.0 6 1369 9 2 2 0 0 1 281 1818 0.0 6 1805 11 1 1 282 14 0.0 6 14 283 3 0.0 6 3 285 2 0.0 6 2 286 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1883 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 1 0.0 6 0 1 61 17 0.0 6 17 69 4 0.0 6 4 70 4 0.0 6 4 71 6 0.0 6 6 72 11 0.0 6 11 73 6 0.0 6 6 74 9 0.0 6 9 75 12 0.0 6 12 76 11 0.0 6 10 1 77 11 0.0 6 11 78 11 0.0 6 11 79 9 0.0 6 9 80 11 0.0 6 11 81 5 0.0 6 4 1 82 3 0.0 6 3 83 3 0.0 6 3 84 7 0.0 6 7 85 3 0.0 6 3 86 2 0.0 6 2 87 2 0.0 6 2 88 3 0.0 6 3 89 5 0.0 6 5 90 6 0.0 6 6 91 8 0.0 6 8 92 6 0.0 6 6 93 4 0.0 6 4 94 3 0.0 6 2 1 95 5 0.0 6 5 96 4 0.0 6 4 97 6 0.0 6 6 98 5 0.0 6 5 99 10 0.0 6 10 100 1 0.0 6 1 101 5 0.0 6 5 102 6 0.0 6 6 103 2 0.0 6 2 104 10 0.0 6 10 105 7 0.0 6 7 106 2 0.0 6 2 107 17 0.0 6 17 108 20 0.0 6 20 109 9 0.0 6 9 110 6 0.0 6 6 111 3 0.0 6 3 112 9 0.0 6 9 113 6 0.0 6 6 114 3 0.0 6 3 115 5 0.0 6 5 116 3 0.0 6 3 117 4 0.0 6 3 1 118 3 0.0 6 3 119 7 0.0 6 7 120 3 0.0 6 3 121 3 0.0 6 3 122 8 0.0 6 8 123 9 0.0 6 9 124 6 0.0 6 6 125 5 0.0 6 5 126 10 0.0 6 10 127 4 0.0 6 4 128 8 0.0 6 8 129 8 0.0 6 8 130 8 0.0 6 8 131 9 0.0 6 9 132 12 0.0 6 12 133 13 0.0 6 13 134 9 0.0 6 9 135 7 0.0 6 7 136 7 0.0 6 7 137 12 0.0 6 12 138 8 0.0 6 8 139 13 0.0 6 13 140 12 0.0 6 12 141 9 0.0 6 9 142 12 0.0 6 12 143 12 0.0 6 12 144 8 0.0 6 8 145 10 0.0 6 10 146 13 0.0 6 13 147 12 0.0 6 12 148 12 0.0 6 12 149 14 0.0 6 14 150 14 0.0 6 14 151 16 0.0 6 16 152 5 0.0 6 5 153 14 0.0 6 14 154 12 0.0 6 11 0 0 0 0 0 1 155 8 0.0 6 8 156 11 0.0 6 11 157 6 0.0 6 6 158 11 0.0 6 11 159 16 0.0 6 16 160 18 0.0 6 18 161 12 0.0 6 12 162 16 0.0 6 16 163 14 0.0 6 14 164 17 0.0 6 17 165 18 0.0 6 18 166 6 0.0 6 6 167 15 0.0 6 15 168 13 0.0 6 13 169 15 0.0 6 15 170 15 0.0 6 15 171 30 0.0 6 30 172 28 0.0 6 28 173 17 0.0 6 17 174 17 0.0 6 17 175 10 0.0 6 9 1 176 12 0.0 6 12 177 18 0.0 6 18 178 14 0.0 6 14 179 21 0.0 6 21 180 19 0.0 6 19 181 13 0.0 6 13 182 15 0.0 6 15 183 28 0.0 6 28 184 9 0.0 6 9 185 17 0.0 6 17 186 15 0.0 6 15 187 12 0.0 6 12 188 8 0.0 6 8 189 21 0.0 6 21 190 14 0.0 6 14 191 31 0.0 6 31 192 16 0.0 6 16 193 11 0.0 6 11 194 10 0.0 6 9 1 195 9 0.0 6 9 196 11 0.0 6 11 197 6 0.0 6 6 198 8 0.0 6 8 199 15 0.0 6 15 200 14 0.0 6 13 1 201 12 0.0 6 12 202 8 0.0 6 8 203 15 0.0 6 14 1 204 14 0.0 6 14 205 15 0.0 6 15 206 14 0.0 6 14 207 16 0.0 6 16 208 8 0.0 6 8 209 12 0.0 6 12 210 9 0.0 6 9 211 5 0.0 6 5 212 7 0.0 6 7 213 11 0.0 6 11 214 8 0.0 6 8 215 14 0.0 6 14 216 9 0.0 6 9 217 8 0.0 6 8 218 9 0.0 6 9 219 15 0.0 6 15 220 8 0.0 6 8 221 12 0.0 6 12 222 18 0.0 6 18 223 9 0.0 6 9 224 10 0.0 6 10 225 4 0.0 6 4 226 4 0.0 6 4 227 13 0.0 6 13 228 12 0.0 6 12 229 3 0.0 6 3 230 6 0.0 6 5 1 231 5 0.0 6 5 232 11 0.0 6 11 233 6 0.0 6 6 234 11 0.0 6 11 235 3 0.0 6 3 236 14 0.0 6 14 237 12 0.0 6 12 238 3 0.0 6 3 239 9 0.0 6 9 240 3 0.0 6 3 241 2 0.0 6 2 242 5 0.0 6 5 243 4 0.0 6 4 244 3 0.0 6 3 245 3 0.0 6 3 246 4 0.0 6 4 247 1 0.0 6 1 248 3 0.0 6 3 249 8 0.0 6 8 250 5 0.0 6 5 251 3 0.0 6 3 252 2 0.0 6 2 253 5 0.0 6 5 254 2 0.0 6 2 255 10 0.0 6 10 256 6 0.0 6 5 1 257 4 0.0 6 4 258 3 0.0 6 3 259 4 0.0 6 4 260 3 0.0 6 3 261 5 0.0 6 5 262 2 0.0 6 2 263 1 0.0 6 1 264 1 0.0 6 1 265 1 0.0 6 1 266 1 0.0 6 1 267 3 0.0 6 3 269 2 0.0 6 2 270 1 0.0 6 1 271 3 0.0 6 3 273 3 0.0 6 3 274 1 0.0 6 1 275 2 0.0 6 2 276 2 0.0 6 2 277 1 0.0 6 1 278 3 0.0 6 3 280 2 0.0 6 2 281 5 0.0 6 5 282 2 0.0 6 2 283 2 0.0 6 2 284 1 0.0 6 1 286 1 0.0 6 1 289 1 0.0 6 1 291 1 0.0 6 1 292 3 0.0 6 3 293 2 0.0 6 2 296 2 0.0 6 2 298 3 0.0 6 3 300 2 0.0 6 2 303 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 71303 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1136.1 0 1 6 1 17.8 1 1 9 2 0.3 2 2 10 12 0.1 3 10 2 11 8 0.0 3 7 1 12 1 0.0 3 0 1 13 14 0.0 3 12 2 14 15 0.0 4 14 0 1 15 3 0.0 4 1 2 16 15 0.0 4 12 3 17 38 0.0 5 32 6 18 25 0.0 5 22 3 19 26 0.0 5 25 1 20 151 0.0 6 147 4 21 95 0.0 6 92 3 22 46 0.0 6 43 3 23 50 0.0 6 46 4 24 40 0.0 6 38 1 0 0 1 25 36 0.0 6 36 26 38 0.0 6 32 5 0 0 1 27 24 0.0 6 23 1 28 26 0.0 6 25 1 29 22 0.0 6 21 1 30 22 0.0 6 20 1 0 1 31 16 0.0 6 15 1 32 26 0.0 6 22 4 33 19 0.0 6 18 1 34 22 0.0 6 20 2 35 18 0.0 6 18 36 19 0.0 6 18 1 37 24 0.0 6 22 2 38 18 0.0 6 13 5 39 18 0.0 6 15 3 40 22 0.0 6 21 1 41 24 0.0 6 23 1 42 17 0.0 6 16 1 43 22 0.0 6 22 44 23 0.0 6 20 3 45 21 0.0 6 21 46 28 0.0 6 25 2 0 0 1 47 24 0.0 6 24 48 28 0.0 6 25 3 49 28 0.0 6 23 4 0 0 0 0 1 50 32 0.0 6 29 1 1 1 51 34 0.0 6 30 4 52 43 0.0 6 42 1 53 38 0.0 6 37 1 54 28 0.0 6 27 1 55 27 0.0 6 24 3 56 35 0.0 6 28 6 1 57 41 0.0 6 37 1 2 1 58 27 0.0 6 24 3 59 33 0.0 6 30 1 2 60 28 0.0 6 25 3 61 27 0.0 6 26 0 0 0 1 62 34 0.0 6 30 1 2 0 0 0 1 63 48 0.0 6 44 4 64 42 0.0 6 40 2 65 46 0.0 6 43 2 0 0 1 66 57 0.0 6 53 4 67 157 0.0 6 146 9 1 0 0 0 1 68 69 0.0 6 64 4 1 69 73 0.0 6 69 3 1 70 48 0.0 6 47 1 71 57 0.0 6 55 2 72 43 0.0 6 39 4 73 38 0.0 6 34 2 0 0 0 2 74 43 0.0 6 40 2 1 75 63 0.0 6 60 3 76 46 0.0 6 43 2 0 1 77 53 0.0 6 51 2 78 63 0.0 6 60 3 79 72 0.0 6 69 2 1 80 72 0.0 6 68 3 0 0 0 0 1 81 69 0.0 6 66 3 82 76 0.0 6 74 1 0 1 83 82 0.0 6 80 2 84 45 0.0 6 43 2 85 42 0.0 6 40 2 86 67 0.0 6 64 3 87 68 0.0 6 60 7 0 0 0 0 1 88 63 0.0 6 59 4 89 70 0.0 6 68 2 90 80 0.0 6 76 3 1 91 73 0.0 6 70 2 0 0 1 92 71 0.0 6 67 4 93 90 0.0 6 85 5 94 98 0.0 6 92 6 95 96 0.0 6 91 5 96 121 0.0 6 112 9 97 112 0.0 6 108 4 98 103 0.0 6 95 8 99 94 0.0 6 90 2 1 0 0 0 1 100 105 0.0 6 98 7 101 125 0.0 6 119 5 0 0 0 1 102 104 0.0 6 99 5 103 145 0.0 6 138 4 1 0 1 0 1 104 226 0.0 6 217 8 0 0 1 105 128 0.0 6 123 3 2 106 124 0.0 6 118 6 107 153 0.0 6 142 10 0 0 1 108 142 0.0 6 136 5 0 0 0 1 109 149 0.0 6 144 4 1 110 130 0.0 6 127 2 1 111 164 0.0 6 154 9 0 1 112 223 0.0 6 212 8 0 1 0 0 2 113 188 0.0 6 184 2 1 0 0 0 1 114 183 0.0 6 170 12 0 0 0 0 1 115 209 0.0 6 204 5 116 190 0.0 6 180 10 117 214 0.0 6 208 6 118 213 0.0 6 205 8 119 190 0.0 6 186 4 120 191 0.0 6 187 4 121 222 0.0 6 214 7 0 1 122 242 0.0 6 234 8 123 241 0.0 6 234 7 124 274 0.0 6 267 7 125 349 0.0 6 337 10 1 0 0 0 1 126 306 0.0 6 298 7 1 127 361 0.0 6 354 7 128 385 0.0 6 378 5 0 0 1 1 129 415 0.0 6 400 14 0 0 0 1 130 454 0.0 6 438 9 2 1 1 1 2 131 488 0.0 6 470 16 0 0 0 1 1 132 440 0.0 6 431 6 0 1 0 1 1 133 549 0.0 6 536 12 1 134 574 0.0 6 560 12 0 1 0 1 135 524 0.0 6 513 8 1 0 1 0 1 136 554 0.0 6 538 12 1 1 1 0 1 137 553 0.0 6 538 10 1 1 2 1 138 661 0.0 6 644 15 0 0 0 1 1 139 734 0.0 6 718 13 1 0 0 0 2 140 875 0.0 6 845 23 3 0 1 1 2 141 837 0.0 6 826 10 0 0 0 0 1 142 963 0.0 6 941 18 2 0 0 1 1 143 892 0.0 6 876 12 1 2 1 144 990 0.0 6 971 17 1 0 0 0 1 145 987 0.0 6 958 24 2 0 1 1 1 146 1058 0.0 6 1034 22 0 0 1 1 147 981 0.0 6 963 16 0 2 148 1243 0.0 6 1214 25 0 2 0 1 1 149 1395 0.0 6 1362 23 5 2 1 0 2 150 1645 0.0 6 1619 23 1 1 0 0 1 151 1238 0.0 6 1213 20 1 1 2 1 152 1575 0.0 6 1536 28 3 2 3 2 1 153 1468 0.0 6 1433 29 1 3 2 154 1251 0.0 6 1224 21 1 0 1 2 2 155 1242 0.0 6 1206 28 1 4 2 0 1 156 1189 0.0 6 1153 28 3 0 2 2 1 157 1338 0.0 6 1312 22 0 0 1 2 1 158 1302 0.0 6 1287 10 2 0 1 2 159 1130 0.0 6 1110 17 1 1 0 0 1 160 1176 0.0 6 1150 24 0 0 0 1 1 161 1042 0.0 6 1027 13 0 0 1 1 162 900 0.0 6 887 10 2 0 1 163 971 0.0 6 953 15 1 1 0 1 164 920 0.0 6 902 11 2 2 0 2 1 165 893 0.0 6 880 9 0 1 2 0 1 166 1090 0.0 6 1072 17 1 167 901 0.0 6 892 7 0 0 0 0 2 168 1072 0.0 6 1061 11 169 951 0.0 6 935 15 0 1 170 934 0.0 6 926 7 0 0 0 0 1 171 1067 0.0 6 1051 10 1 1 1 3 172 923 0.0 6 908 12 0 0 1 2 173 1059 0.0 6 1049 8 1 1 174 900 0.0 6 883 15 2 175 983 0.0 6 970 9 0 1 2 0 1 176 1122 0.0 6 1114 7 1 177 794 0.0 6 784 7 1 1 0 1 178 743 0.0 6 736 7 179 624 0.0 6 618 6 180 574 0.0 6 565 8 1 181 621 0.0 6 613 7 0 0 0 1 182 559 0.0 6 552 6 0 0 0 0 1 183 791 0.0 6 784 5 0 2 184 981 0.0 6 969 10 0 0 0 1 1 185 861 0.0 6 846 13 1 0 1 186 624 0.0 6 617 7 187 577 0.0 6 570 5 0 0 0 1 1 188 540 0.0 6 535 5 189 475 0.0 6 472 3 190 481 0.0 6 477 4 191 488 0.0 6 482 5 0 0 0 1 192 366 0.0 6 364 1 1 193 332 0.0 6 331 1 194 324 0.0 6 321 1 1 0 1 195 263 0.0 6 262 1 196 347 0.0 6 344 2 0 1 197 312 0.0 6 308 4 198 256 0.0 6 253 1 1 0 0 0 1 199 213 0.0 6 212 1 200 224 0.0 6 222 2 201 177 0.0 6 174 3 202 179 0.0 6 176 2 0 0 1 203 143 0.0 6 143 204 135 0.0 6 134 1 205 143 0.0 6 142 1 206 157 0.0 6 156 1 207 142 0.0 6 141 1 208 107 0.0 6 107 209 97 0.0 6 95 2 210 91 0.0 6 88 2 0 0 1 211 95 0.0 6 95 212 73 0.0 6 73 213 91 0.0 6 90 0 0 0 0 0 1 214 85 0.0 6 85 215 78 0.0 6 77 1 216 63 0.0 6 63 217 58 0.0 6 58 218 46 0.0 6 46 219 50 0.0 6 50 220 28 0.0 6 28 221 46 0.0 6 46 222 40 0.0 6 39 1 223 22 0.0 6 21 1 224 25 0.0 6 25 225 21 0.0 6 21 226 14 0.0 6 14 227 15 0.0 6 14 1 228 20 0.0 6 20 229 11 0.0 6 11 230 7 0.0 6 7 231 14 0.0 6 13 1 232 5 0.0 6 5 233 8 0.0 6 8 234 2 0.0 6 2 235 5 0.0 6 5 236 2 0.0 6 2 237 2 0.0 6 2 239 3 0.0 6 3 240 2 0.0 6 2 241 3 0.0 6 3 242 4 0.0 6 4 246 1 0.0 6 1 247 2 0.0 6 2 248 3 0.0 6 3 253 1 0.0 6 1 276 1 0.0 6 1 277 3 0.0 6 3 278 14 0.0 6 14 279 108 0.0 6 105 2 0 1 281 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1338 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 52 1 0.0 6 1 56 2 0.0 6 2 62 1 0.0 6 1 65 1 0.0 6 1 68 1 0.0 6 1 70 1 0.0 6 1 71 2 0.0 6 2 72 9 0.0 6 9 73 6 0.0 6 6 74 9 0.0 6 9 75 9 0.0 6 9 76 56 0.0 6 55 0 0 1 77 5 0.0 6 5 78 10 0.0 6 10 79 9 0.0 6 8 1 80 20 0.0 6 20 81 7 0.0 6 7 82 8 0.0 6 8 83 5 0.0 6 5 84 11 0.0 6 11 85 4 0.0 6 4 86 8 0.0 6 7 1 87 4 0.0 6 4 88 6 0.0 6 5 1 89 4 0.0 6 4 90 2 0.0 6 2 91 9 0.0 6 9 92 3 0.0 6 3 93 7 0.0 6 7 94 5 0.0 6 5 95 7 0.0 6 6 0 0 1 96 7 0.0 6 7 97 10 0.0 6 10 98 13 0.0 6 13 99 7 0.0 6 7 100 5 0.0 6 5 101 6 0.0 6 6 102 2 0.0 6 2 103 5 0.0 6 5 104 8 0.0 6 8 105 9 0.0 6 9 106 8 0.0 6 8 107 4 0.0 6 4 108 4 0.0 6 4 109 8 0.0 6 8 110 45 0.0 6 45 111 10 0.0 6 10 112 3 0.0 6 3 113 6 0.0 6 6 114 9 0.0 6 9 115 4 0.0 6 4 116 3 0.0 6 3 117 4 0.0 6 4 118 3 0.0 6 3 119 2 0.0 6 2 120 6 0.0 6 5 0 0 0 1 121 2 0.0 6 2 122 4 0.0 6 4 123 9 0.0 6 9 124 5 0.0 6 5 125 8 0.0 6 8 126 13 0.0 6 13 127 11 0.0 6 11 128 14 0.0 6 14 129 7 0.0 6 7 130 3 0.0 6 3 131 7 0.0 6 7 132 10 0.0 6 10 133 10 0.0 6 10 134 12 0.0 6 12 135 9 0.0 6 9 136 13 0.0 6 13 137 14 0.0 6 14 138 8 0.0 6 7 0 0 1 139 10 0.0 6 10 140 18 0.0 6 18 141 8 0.0 6 8 142 17 0.0 6 17 143 11 0.0 6 10 1 144 7 0.0 6 7 145 12 0.0 6 12 146 7 0.0 6 7 147 20 0.0 6 19 1 148 12 0.0 6 12 149 13 0.0 6 13 150 7 0.0 6 7 151 12 0.0 6 12 152 9 0.0 6 9 153 12 0.0 6 12 154 8 0.0 6 8 155 8 0.0 6 8 156 7 0.0 6 7 157 10 0.0 6 10 158 25 0.0 6 25 159 8 0.0 6 8 160 5 0.0 6 4 0 0 1 161 12 0.0 6 12 162 13 0.0 6 13 163 21 0.0 6 21 164 7 0.0 6 7 165 11 0.0 6 11 166 12 0.0 6 12 167 26 0.0 6 26 168 10 0.0 6 10 169 11 0.0 6 11 170 21 0.0 6 21 171 21 0.0 6 21 172 10 0.0 6 10 173 26 0.0 6 26 174 30 0.0 6 30 175 8 0.0 6 8 176 12 0.0 6 12 177 9 0.0 6 9 178 14 0.0 6 14 179 13 0.0 6 12 1 180 4 0.0 6 4 181 14 0.0 6 14 182 7 0.0 6 7 183 4 0.0 6 4 184 8 0.0 6 8 185 6 0.0 6 6 186 6 0.0 6 6 187 9 0.0 6 9 188 10 0.0 6 10 189 7 0.0 6 7 190 10 0.0 6 10 191 16 0.0 6 16 192 5 0.0 6 5 193 6 0.0 6 6 194 8 0.0 6 8 195 7 0.0 6 7 196 3 0.0 6 3 197 3 0.0 6 3 198 7 0.0 6 7 199 7 0.0 6 7 200 2 0.0 6 2 201 4 0.0 6 3 1 202 2 0.0 6 2 203 5 0.0 6 5 204 2 0.0 6 2 205 3 0.0 6 3 206 2 0.0 6 2 207 5 0.0 6 5 208 4 0.0 6 3 0 0 0 1 209 4 0.0 6 4 210 2 0.0 6 2 211 1 0.0 6 1 212 3 0.0 6 3 213 4 0.0 6 4 214 2 0.0 6 2 215 1 0.0 6 1 216 2 0.0 6 2 217 2 0.0 6 2 218 3 0.0 6 3 219 1 0.0 6 1 220 2 0.0 6 2 221 2 0.0 6 2 222 3 0.0 6 3 223 1 0.0 6 1 224 4 0.0 6 4 225 4 0.0 6 2 0 0 2 228 5 0.0 6 4 0 0 0 1 238 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_04_63_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_04_290_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_04_63_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_04_290_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.19 s (41 µs/read; 1.46 M reads/minute). === Summary === Total read pairs processed: 28,858 Read 1 with adapter: 28,857 (100.0%) Read 2 with adapter: 28,853 (100.0%) == Read fate breakdown == Pairs that were too short: 14 (0.0%) Pairs discarded as untrimmed: 6 (0.0%) Pairs written (passing filters): 28,838 (99.9%) Total basepairs processed: 13,086,461 bp Read 1: 7,601,426 bp Read 2: 5,485,035 bp Total written (filtered): 1,713,657 bp (13.1%) Read 1: 874,320 bp Read 2: 839,337 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 28300 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 450.9 0 1 6 2 7.0 1 1 0 1 16 1 0.0 4 0 1 17 1 0.0 5 1 19 2 0.0 5 1 1 20 44 0.0 6 42 2 21 16 0.0 6 16 22 14 0.0 6 13 1 23 8 0.0 6 8 24 1 0.0 6 1 25 2 0.0 6 2 26 5 0.0 6 5 27 8 0.0 6 8 28 1 0.0 6 1 29 2 0.0 6 2 30 1 0.0 6 1 31 2 0.0 6 2 32 2 0.0 6 2 33 4 0.0 6 3 1 35 1 0.0 6 1 36 2 0.0 6 2 37 3 0.0 6 3 38 1 0.0 6 1 39 1 0.0 6 1 40 2 0.0 6 1 0 1 42 2 0.0 6 2 43 4 0.0 6 4 44 5 0.0 6 5 45 3 0.0 6 3 47 1 0.0 6 1 48 1 0.0 6 1 49 3 0.0 6 3 50 6 0.0 6 6 51 8 0.0 6 8 52 4 0.0 6 4 53 3 0.0 6 3 54 6 0.0 6 6 55 3 0.0 6 3 57 3 0.0 6 2 1 58 2 0.0 6 2 59 2 0.0 6 2 60 3 0.0 6 3 61 1 0.0 6 1 63 3 0.0 6 3 64 2 0.0 6 1 1 65 8 0.0 6 8 66 4 0.0 6 3 1 67 5 0.0 6 5 68 5 0.0 6 5 69 6 0.0 6 5 1 70 2 0.0 6 2 71 8 0.0 6 8 72 5 0.0 6 5 73 8 0.0 6 8 74 8 0.0 6 8 75 3 0.0 6 3 76 2 0.0 6 2 77 8 0.0 6 8 78 3 0.0 6 3 79 4 0.0 6 4 80 6 0.0 6 6 81 2 0.0 6 2 82 7 0.0 6 7 83 1 0.0 6 1 84 2 0.0 6 2 85 8 0.0 6 7 1 86 8 0.0 6 8 87 3 0.0 6 3 88 1 0.0 6 1 89 5 0.0 6 5 90 5 0.0 6 5 91 6 0.0 6 4 2 92 2 0.0 6 1 1 93 8 0.0 6 8 94 3 0.0 6 3 95 5 0.0 6 4 1 96 2 0.0 6 2 97 8 0.0 6 8 98 3 0.0 6 2 1 99 3 0.0 6 3 100 5 0.0 6 5 101 3 0.0 6 3 102 5 0.0 6 5 103 2 0.0 6 2 104 12 0.0 6 12 105 6 0.0 6 5 1 106 8 0.0 6 7 1 107 8 0.0 6 8 108 4 0.0 6 3 1 109 4 0.0 6 4 110 1 0.0 6 1 111 7 0.0 6 7 112 6 0.0 6 6 113 4 0.0 6 4 114 1 0.0 6 1 115 5 0.0 6 5 116 9 0.0 6 9 117 8 0.0 6 6 1 0 0 0 1 118 8 0.0 6 7 0 0 0 0 0 1 119 14 0.0 6 13 1 120 4 0.0 6 4 121 6 0.0 6 6 122 11 0.0 6 10 1 123 11 0.0 6 11 124 17 0.0 6 16 1 125 8 0.0 6 7 1 126 7 0.0 6 7 127 4 0.0 6 4 128 10 0.0 6 10 129 3 0.0 6 3 130 15 0.0 6 15 131 10 0.0 6 10 132 13 0.0 6 12 1 133 13 0.0 6 11 2 134 17 0.0 6 16 0 0 1 135 11 0.0 6 10 1 136 14 0.0 6 11 3 137 12 0.0 6 10 1 1 138 7 0.0 6 6 1 139 9 0.0 6 9 140 20 0.0 6 20 141 14 0.0 6 13 1 142 151 0.0 6 151 143 34 0.0 6 33 1 144 29 0.0 6 28 1 145 18 0.0 6 17 1 146 23 0.0 6 22 1 147 13 0.0 6 12 1 148 15 0.0 6 15 149 14 0.0 6 14 150 8 0.0 6 8 151 16 0.0 6 16 152 20 0.0 6 20 153 24 0.0 6 22 1 0 0 0 1 154 19 0.0 6 18 1 155 16 0.0 6 15 1 156 17 0.0 6 17 157 18 0.0 6 18 158 33 0.0 6 33 159 71 0.0 6 71 160 26 0.0 6 24 2 161 27 0.0 6 26 1 162 30 0.0 6 29 1 163 31 0.0 6 28 3 164 27 0.0 6 26 1 165 38 0.0 6 37 1 166 29 0.0 6 28 1 167 33 0.0 6 32 1 168 28 0.0 6 28 169 21 0.0 6 21 170 38 0.0 6 37 0 0 1 171 43 0.0 6 43 172 42 0.0 6 41 1 173 37 0.0 6 37 174 42 0.0 6 42 175 44 0.0 6 43 1 176 49 0.0 6 48 1 177 93 0.0 6 90 2 0 0 1 178 451 0.0 6 450 1 179 88 0.0 6 86 2 180 34 0.0 6 33 1 181 45 0.0 6 45 182 35 0.0 6 34 1 183 59 0.0 6 55 3 1 184 37 0.0 6 36 1 185 58 0.0 6 54 4 186 42 0.0 6 40 2 187 32 0.0 6 32 188 30 0.0 6 30 189 44 0.0 6 40 4 190 70 0.0 6 69 1 191 53 0.0 6 51 2 192 52 0.0 6 50 2 193 104 0.0 6 103 1 194 117 0.0 6 114 3 195 153 0.0 6 147 5 1 196 125 0.0 6 121 3 0 0 0 1 197 169 0.0 6 169 198 192 0.0 6 191 1 199 211 0.0 6 209 2 200 216 0.0 6 210 5 0 0 0 0 1 201 184 0.0 6 178 6 202 219 0.0 6 215 4 203 158 0.0 6 153 5 204 201 0.0 6 199 2 205 320 0.0 6 309 11 206 134 0.0 6 131 2 0 0 0 1 207 229 0.0 6 227 2 208 296 0.0 6 288 7 1 209 155 0.0 6 153 2 210 309 0.0 6 304 4 0 0 0 0 1 211 238 0.0 6 234 3 0 0 0 0 1 212 241 0.0 6 237 4 213 210 0.0 6 205 5 214 232 0.0 6 228 4 215 276 0.0 6 269 7 216 415 0.0 6 411 2 1 0 0 0 1 217 293 0.0 6 286 6 1 218 174 0.0 6 169 3 0 1 0 0 1 219 302 0.0 6 297 4 0 0 0 0 1 220 240 0.0 6 237 2 0 0 0 0 1 221 291 0.0 6 288 3 222 144 0.0 6 141 2 1 223 217 0.0 6 212 5 224 284 0.0 6 273 9 1 0 0 0 1 225 285 0.0 6 276 8 0 0 0 1 226 255 0.0 6 252 2 0 0 0 0 1 227 268 0.0 6 265 3 228 274 0.0 6 268 5 0 0 0 1 229 201 0.0 6 195 6 230 227 0.0 6 223 4 231 260 0.0 6 259 1 232 232 0.0 6 229 3 233 299 0.0 6 291 7 0 0 0 0 1 234 255 0.0 6 248 6 0 0 0 0 1 235 275 0.0 6 273 1 0 0 0 0 1 236 283 0.0 6 277 6 237 297 0.0 6 294 3 238 181 0.0 6 174 7 239 198 0.0 6 195 3 240 311 0.0 6 304 5 0 0 0 0 2 241 268 0.0 6 259 7 0 0 0 0 2 242 362 0.0 6 357 5 243 292 0.0 6 287 4 0 1 244 220 0.0 6 212 7 0 1 245 203 0.0 6 202 1 246 150 0.0 6 147 2 1 247 176 0.0 6 172 3 0 0 0 0 1 248 265 0.0 6 259 4 1 0 0 0 1 249 248 0.0 6 237 10 0 0 0 0 1 250 325 0.0 6 322 3 251 336 0.0 6 329 6 0 0 0 1 252 456 0.0 6 450 5 1 253 507 0.0 6 499 7 0 0 0 1 254 444 0.0 6 437 7 255 348 0.0 6 340 7 0 0 0 0 1 256 434 0.0 6 425 8 0 0 0 0 1 257 409 0.0 6 403 6 258 515 0.0 6 508 7 259 642 0.0 6 637 4 0 0 0 0 1 260 679 0.0 6 676 3 261 248 0.0 6 246 2 262 255 0.0 6 252 2 0 0 1 263 212 0.0 6 204 6 1 0 0 0 1 264 386 0.0 6 382 4 265 433 0.0 6 429 3 0 0 0 0 1 266 124 0.0 6 121 2 0 0 0 0 1 267 93 0.0 6 90 3 268 201 0.0 6 197 4 269 95 0.0 6 95 270 106 0.0 6 104 2 271 115 0.0 6 113 2 272 141 0.0 6 137 3 0 1 273 115 0.0 6 110 5 274 126 0.0 6 125 1 275 208 0.0 6 202 6 276 244 0.0 6 238 6 277 243 0.0 6 239 4 278 295 0.0 6 294 1 279 275 0.0 6 269 5 1 280 1074 0.0 6 1066 7 0 0 0 0 1 281 1805 0.0 6 1755 49 0 0 0 0 1 282 13 0.0 6 10 2 1 283 1 0.0 6 1 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 557 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 1 0.0 6 1 72 8 0.0 6 8 84 1 0.0 6 1 86 1 0.0 6 0 1 88 1 0.0 6 1 89 7 0.0 6 7 91 2 0.0 6 2 104 2 0.0 6 2 106 1 0.0 6 0 1 107 3 0.0 6 3 108 18 0.0 6 18 115 1 0.0 6 1 134 1 0.0 6 1 135 9 0.0 6 9 140 1 0.0 6 1 141 7 0.0 6 7 168 8 0.0 6 8 219 1 0.0 6 1 221 3 0.0 6 3 222 42 0.0 6 42 223 132 0.0 6 132 224 3 0.0 6 3 225 1 0.0 6 1 226 2 0.0 6 2 227 47 0.0 6 47 228 143 0.0 6 143 229 6 0.0 6 6 239 4 0.0 6 4 240 13 0.0 6 13 241 1 0.0 6 1 244 1 0.0 6 1 246 5 0.0 6 5 247 26 0.0 6 26 248 1 0.0 6 1 268 5 0.0 6 5 269 7 0.0 6 7 281 1 0.0 6 1 282 2 0.0 6 2 296 2 0.0 6 2 297 23 0.0 6 23 298 14 0.0 6 14 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 28749 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.8% G: 0.1% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 1.8 2 0 1 9 2 0.1 2 2 11 2 0.0 3 2 12 1 0.0 3 1 13 1 0.0 3 1 14 3 0.0 4 2 1 16 2 0.0 4 2 17 8 0.0 5 8 19 33 0.0 5 31 2 20 52 0.0 6 50 1 1 21 18 0.0 6 17 1 22 17 0.0 6 16 1 23 12 0.0 6 12 24 11 0.0 6 11 25 7 0.0 6 7 26 17 0.0 6 14 3 27 12 0.0 6 9 3 28 18 0.0 6 17 1 29 5 0.0 6 5 30 7 0.0 6 6 1 31 4 0.0 6 4 32 9 0.0 6 9 33 10 0.0 6 10 34 8 0.0 6 8 35 6 0.0 6 6 36 1 0.0 6 1 37 3 0.0 6 2 1 38 2 0.0 6 2 39 8 0.0 6 6 0 0 0 0 2 40 7 0.0 6 4 3 41 4 0.0 6 3 1 42 6 0.0 6 6 43 5 0.0 6 5 44 13 0.0 6 10 2 1 45 10 0.0 6 10 46 9 0.0 6 8 1 47 10 0.0 6 7 0 2 0 1 48 8 0.0 6 7 1 49 7 0.0 6 5 0 0 0 0 2 50 6 0.0 6 5 0 0 0 0 0 1 51 19 0.0 6 16 1 1 1 52 12 0.0 6 10 2 53 12 0.0 6 12 54 2 0.0 6 2 55 11 0.0 6 11 56 9 0.0 6 9 57 7 0.0 6 6 1 58 8 0.0 6 6 1 1 59 10 0.0 6 10 60 9 0.0 6 7 0 1 0 1 61 10 0.0 6 10 62 6 0.0 6 5 0 1 63 15 0.0 6 13 0 0 0 0 2 64 21 0.0 6 20 0 1 65 13 0.0 6 12 1 66 27 0.0 6 26 0 0 0 0 0 1 67 20 0.0 6 19 1 68 19 0.0 6 17 1 0 0 0 1 69 23 0.0 6 19 3 0 1 70 50 0.0 6 48 2 71 20 0.0 6 16 2 2 72 30 0.0 6 27 3 73 16 0.0 6 15 1 74 23 0.0 6 22 0 0 0 0 0 1 75 20 0.0 6 16 4 76 16 0.0 6 15 1 77 19 0.0 6 16 3 78 13 0.0 6 13 79 16 0.0 6 15 1 80 14 0.0 6 14 81 22 0.0 6 20 1 1 82 13 0.0 6 11 2 83 22 0.0 6 21 0 1 84 21 0.0 6 18 2 0 0 0 1 85 16 0.0 6 15 0 0 0 0 1 86 22 0.0 6 21 1 87 18 0.0 6 16 1 0 0 0 1 88 11 0.0 6 9 2 89 25 0.0 6 23 1 0 0 0 1 90 26 0.0 6 25 1 91 23 0.0 6 20 3 92 20 0.0 6 19 1 93 33 0.0 6 28 3 0 2 94 30 0.0 6 28 1 0 0 0 1 95 32 0.0 6 31 1 96 32 0.0 6 30 2 97 41 0.0 6 35 4 1 0 0 1 98 49 0.0 6 45 2 0 0 0 2 99 43 0.0 6 36 6 0 1 100 43 0.0 6 41 2 101 36 0.0 6 35 1 102 33 0.0 6 33 103 50 0.0 6 44 5 0 0 0 0 1 104 48 0.0 6 44 3 0 1 105 35 0.0 6 34 1 106 89 0.0 6 86 2 0 0 0 0 1 107 114 0.0 6 108 4 0 1 1 108 52 0.0 6 44 8 109 56 0.0 6 55 1 110 50 0.0 6 45 3 0 0 0 1 1 111 53 0.0 6 49 3 1 112 39 0.0 6 35 3 1 113 78 0.0 6 77 1 114 48 0.0 6 45 3 115 61 0.0 6 58 3 116 52 0.0 6 51 1 117 48 0.0 6 45 1 0 0 0 1 1 118 78 0.0 6 74 4 119 59 0.0 6 56 2 1 120 87 0.0 6 81 6 121 79 0.0 6 76 2 0 0 1 122 60 0.0 6 57 3 123 80 0.0 6 77 3 124 68 0.0 6 63 3 1 0 0 1 125 58 0.0 6 55 2 1 126 79 0.0 6 73 5 1 127 100 0.0 6 95 4 1 128 242 0.0 6 233 8 0 1 129 119 0.0 6 115 4 130 130 0.0 6 128 2 131 138 0.0 6 132 4 1 0 0 1 132 106 0.0 6 103 2 1 133 160 0.0 6 155 4 1 134 241 0.0 6 232 8 1 135 236 0.0 6 224 11 0 0 0 1 136 213 0.0 6 201 11 0 1 137 288 0.0 6 278 9 0 1 138 277 0.0 6 263 13 0 1 139 228 0.0 6 222 6 140 283 0.0 6 276 7 141 159 0.0 6 151 7 0 0 1 142 211 0.0 6 208 1 1 1 143 239 0.0 6 227 11 1 144 266 0.0 6 254 11 0 1 145 305 0.0 6 293 12 146 210 0.0 6 200 7 1 1 0 1 147 251 0.0 6 238 11 1 1 148 318 0.0 6 305 13 149 476 0.0 6 449 23 0 0 1 3 150 450 0.0 6 423 25 1 1 151 673 0.0 6 640 28 1 2 0 2 152 1123 0.0 6 1074 44 1 2 0 2 153 618 0.0 6 594 22 0 1 0 1 154 660 0.0 6 634 26 155 736 0.0 6 708 25 1 1 0 1 156 639 0.0 6 603 33 0 0 0 3 157 774 0.0 6 743 28 1 1 0 1 158 761 0.0 6 722 34 2 2 0 1 159 402 0.0 6 390 9 0 1 1 1 160 522 0.0 6 503 17 1 0 0 1 161 366 0.0 6 345 19 2 162 238 0.0 6 223 14 0 1 163 322 0.0 6 317 5 164 160 0.0 6 152 6 0 0 1 1 165 218 0.0 6 209 9 166 195 0.0 6 192 2 0 1 167 262 0.0 6 256 4 2 168 173 0.0 6 171 1 1 169 200 0.0 6 191 9 170 181 0.0 6 174 7 171 314 0.0 6 302 11 0 0 0 1 172 268 0.0 6 255 11 1 0 0 1 173 182 0.0 6 171 10 1 174 268 0.0 6 257 9 1 1 175 392 0.0 6 376 14 0 0 1 1 176 347 0.0 6 336 10 0 0 0 1 177 353 0.0 6 341 10 1 1 178 319 0.0 6 305 12 1 1 179 302 0.0 6 288 12 1 0 0 1 180 218 0.0 6 208 9 1 181 318 0.0 6 306 12 182 179 0.0 6 174 5 183 112 0.0 6 106 4 1 0 0 1 184 98 0.0 6 97 1 185 92 0.0 6 84 6 0 1 0 1 186 401 0.0 6 387 13 1 187 707 0.0 6 667 38 1 0 0 1 188 528 0.0 6 503 21 2 1 1 189 275 0.0 6 263 11 0 0 0 1 190 211 0.0 6 203 8 191 269 0.0 6 255 13 1 192 168 0.0 6 160 8 193 246 0.0 6 238 8 194 228 0.0 6 223 5 195 172 0.0 6 168 4 196 280 0.0 6 274 6 197 131 0.0 6 130 1 198 219 0.0 6 212 7 199 299 0.0 6 294 5 200 98 0.0 6 95 3 201 215 0.0 6 210 4 0 0 0 1 202 99 0.0 6 96 3 203 90 0.0 6 88 2 204 115 0.0 6 109 4 0 0 2 205 135 0.0 6 128 6 0 1 206 57 0.0 6 56 1 207 68 0.0 6 66 2 208 123 0.0 6 118 5 209 80 0.0 6 77 3 210 63 0.0 6 59 3 0 1 211 94 0.0 6 91 3 212 72 0.0 6 68 4 213 63 0.0 6 60 3 214 65 0.0 6 65 215 83 0.0 6 81 2 216 82 0.0 6 80 2 217 74 0.0 6 73 1 218 51 0.0 6 50 1 219 62 0.0 6 59 2 1 220 33 0.0 6 31 2 221 40 0.0 6 38 2 222 77 0.0 6 73 3 1 223 86 0.0 6 85 1 224 118 0.0 6 112 6 225 80 0.0 6 77 3 226 53 0.0 6 49 4 227 157 0.0 6 151 5 0 0 1 228 52 0.0 6 50 2 229 84 0.0 6 81 3 230 21 0.0 6 21 231 15 0.0 6 14 1 232 26 0.0 6 25 1 233 30 0.0 6 28 2 234 40 0.0 6 40 235 35 0.0 6 32 3 236 8 0.0 6 8 237 6 0.0 6 6 238 11 0.0 6 11 239 5 0.0 6 5 240 4 0.0 6 4 241 12 0.0 6 11 1 242 20 0.0 6 19 1 243 10 0.0 6 10 244 37 0.0 6 37 245 8 0.0 6 6 2 246 14 0.0 6 14 247 16 0.0 6 16 248 8 0.0 6 8 249 9 0.0 6 9 250 8 0.0 6 8 251 19 0.0 6 18 1 252 9 0.0 6 8 1 253 10 0.0 6 10 254 13 0.0 6 11 2 256 1 0.0 6 1 278 1 0.0 6 1 280 2 0.0 6 2 281 16 0.0 6 16 282 14 0.0 6 14 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 104 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 14 1 0.0 4 0 0 0 0 1 46 1 0.0 6 0 1 70 2 0.0 6 2 71 7 0.0 6 7 73 1 0.0 6 0 1 88 6 0.0 6 6 90 1 0.0 6 1 94 1 0.0 6 0 0 0 0 1 106 7 0.0 6 7 107 22 0.0 6 22 108 1 0.0 6 1 114 1 0.0 6 1 133 1 0.0 6 1 134 7 0.0 6 7 135 1 0.0 6 1 139 1 0.0 6 1 140 15 0.0 6 15 162 1 0.0 6 1 167 3 0.0 6 3 221 2 0.0 6 2 222 12 0.0 6 12 225 2 0.0 6 1 1 226 3 0.0 6 3 227 5 0.0 6 5 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_05_64_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_05_291_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_05_64_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_05_291_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.72 s (22 µs/read; 2.78 M reads/minute). === Summary === Total read pairs processed: 79,862 Read 1 with adapter: 79,860 (100.0%) Read 2 with adapter: 79,801 (99.9%) == Read fate breakdown == Pairs that were too short: 5 (0.0%) Pairs discarded as untrimmed: 63 (0.1%) Pairs written (passing filters): 79,794 (99.9%) Total basepairs processed: 39,309,768 bp Read 1: 22,631,313 bp Read 2: 16,678,455 bp Total written (filtered): 4,804,609 bp (12.2%) Read 1: 2,405,526 bp Read 2: 2,399,083 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 79622 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 312.0 1 1 6 3 19.5 1 0 0 3 7 3 4.9 2 1 1 1 8 1 1.2 2 1 10 1 0.1 3 1 14 1 0.0 4 0 1 16 1 0.0 4 1 17 1 0.0 5 1 20 32 0.0 6 31 1 21 20 0.0 6 17 3 22 13 0.0 6 13 23 12 0.0 6 12 24 5 0.0 6 5 25 10 0.0 6 10 26 9 0.0 6 9 27 4 0.0 6 4 28 4 0.0 6 4 29 2 0.0 6 2 30 2 0.0 6 2 31 1 0.0 6 1 33 4 0.0 6 4 34 4 0.0 6 4 37 3 0.0 6 3 38 3 0.0 6 3 39 3 0.0 6 3 40 5 0.0 6 5 41 4 0.0 6 4 42 2 0.0 6 2 43 2 0.0 6 2 44 8 0.0 6 8 45 5 0.0 6 5 46 3 0.0 6 3 47 1 0.0 6 1 48 2 0.0 6 2 49 2 0.0 6 2 50 10 0.0 6 8 2 51 2 0.0 6 2 52 4 0.0 6 3 1 53 2 0.0 6 2 54 2 0.0 6 1 1 55 4 0.0 6 2 2 56 5 0.0 6 5 57 1 0.0 6 1 58 6 0.0 6 6 59 2 0.0 6 2 60 2 0.0 6 1 0 1 61 6 0.0 6 6 62 7 0.0 6 7 63 6 0.0 6 6 64 3 0.0 6 3 65 1 0.0 6 1 66 3 0.0 6 3 67 5 0.0 6 5 68 1 0.0 6 1 69 2 0.0 6 2 70 3 0.0 6 3 71 1 0.0 6 1 72 4 0.0 6 3 0 0 0 0 1 73 1 0.0 6 1 74 2 0.0 6 2 75 4 0.0 6 4 76 2 0.0 6 2 77 5 0.0 6 4 1 78 6 0.0 6 5 0 1 79 3 0.0 6 3 80 1 0.0 6 1 81 1 0.0 6 1 82 3 0.0 6 2 1 83 1 0.0 6 1 84 2 0.0 6 2 85 6 0.0 6 6 86 8 0.0 6 8 87 6 0.0 6 6 88 4 0.0 6 4 89 5 0.0 6 4 1 90 3 0.0 6 3 92 5 0.0 6 5 93 1 0.0 6 1 94 2 0.0 6 2 95 1 0.0 6 1 96 2 0.0 6 2 97 5 0.0 6 5 98 10 0.0 6 10 99 4 0.0 6 4 100 7 0.0 6 6 1 101 3 0.0 6 3 102 4 0.0 6 3 0 1 103 7 0.0 6 6 1 104 9 0.0 6 9 105 4 0.0 6 4 106 12 0.0 6 9 3 107 7 0.0 6 5 2 108 13 0.0 6 11 2 109 12 0.0 6 12 110 4 0.0 6 3 1 111 5 0.0 6 5 112 8 0.0 6 8 113 10 0.0 6 9 1 114 14 0.0 6 14 115 13 0.0 6 13 116 10 0.0 6 10 117 15 0.0 6 14 0 1 118 6 0.0 6 6 119 9 0.0 6 9 120 4 0.0 6 4 121 10 0.0 6 10 122 16 0.0 6 15 1 123 16 0.0 6 16 124 10 0.0 6 9 0 1 125 17 0.0 6 17 126 19 0.0 6 18 1 127 7 0.0 6 7 128 16 0.0 6 16 129 11 0.0 6 11 130 8 0.0 6 8 131 9 0.0 6 9 132 12 0.0 6 11 0 0 0 0 0 1 133 18 0.0 6 17 1 134 12 0.0 6 12 135 39 0.0 6 39 136 15 0.0 6 15 137 55 0.0 6 55 138 42 0.0 6 39 2 1 139 19 0.0 6 17 2 140 31 0.0 6 30 1 141 75 0.0 6 74 1 142 97 0.0 6 96 1 143 136 0.0 6 132 4 144 88 0.0 6 88 145 85 0.0 6 83 2 146 138 0.0 6 137 1 147 123 0.0 6 123 148 122 0.0 6 122 149 113 0.0 6 110 2 1 150 98 0.0 6 96 2 151 97 0.0 6 96 1 152 95 0.0 6 95 153 68 0.0 6 65 2 1 154 89 0.0 6 89 155 70 0.0 6 69 1 156 79 0.0 6 77 2 157 59 0.0 6 57 2 158 84 0.0 6 84 159 65 0.0 6 64 1 160 85 0.0 6 85 161 80 0.0 6 76 4 162 94 0.0 6 91 2 1 163 102 0.0 6 101 1 164 84 0.0 6 84 165 61 0.0 6 58 3 166 64 0.0 6 63 1 167 88 0.0 6 88 168 61 0.0 6 61 169 80 0.0 6 79 1 170 114 0.0 6 113 0 1 171 74 0.0 6 74 172 78 0.0 6 78 173 84 0.0 6 83 1 174 116 0.0 6 113 3 175 105 0.0 6 102 3 176 95 0.0 6 94 0 0 0 0 1 177 109 0.0 6 106 3 178 88 0.0 6 86 2 179 178 0.0 6 177 1 180 84 0.0 6 83 1 181 98 0.0 6 94 4 182 128 0.0 6 126 1 1 183 105 0.0 6 105 184 131 0.0 6 124 6 0 0 0 1 185 174 0.0 6 171 3 186 199 0.0 6 193 5 0 0 1 187 185 0.0 6 183 2 188 158 0.0 6 156 2 189 119 0.0 6 115 3 1 190 175 0.0 6 173 2 191 206 0.0 6 205 1 192 169 0.0 6 167 2 193 201 0.0 6 195 6 194 225 0.0 6 214 10 1 195 214 0.0 6 209 5 196 233 0.0 6 229 4 197 233 0.0 6 230 3 198 282 0.0 6 268 12 2 199 329 0.0 6 318 11 200 364 0.0 6 354 10 201 274 0.0 6 269 5 202 274 0.0 6 266 8 203 333 0.0 6 332 1 204 327 0.0 6 321 5 0 0 0 1 205 357 0.0 6 351 6 206 279 0.0 6 273 6 207 355 0.0 6 345 10 208 374 0.0 6 366 7 1 209 327 0.0 6 319 8 210 290 0.0 6 283 6 1 211 193 0.0 6 192 1 212 157 0.0 6 154 3 213 334 0.0 6 328 5 0 0 1 214 392 0.0 6 386 5 1 215 332 0.0 6 326 6 216 331 0.0 6 323 8 217 457 0.0 6 450 7 218 306 0.0 6 299 7 219 316 0.0 6 311 5 220 291 0.0 6 282 9 221 315 0.0 6 307 8 222 358 0.0 6 352 5 0 0 1 223 368 0.0 6 364 4 224 325 0.0 6 321 4 225 354 0.0 6 346 8 226 468 0.0 6 452 16 227 466 0.0 6 458 7 1 228 421 0.0 6 415 6 229 332 0.0 6 320 12 230 384 0.0 6 373 11 231 311 0.0 6 299 12 232 386 0.0 6 375 11 233 371 0.0 6 363 8 234 392 0.0 6 387 5 235 339 0.0 6 333 5 1 236 326 0.0 6 319 6 1 237 350 0.0 6 345 5 238 470 0.0 6 460 8 1 0 1 239 436 0.0 6 427 8 0 1 240 459 0.0 6 444 14 1 241 513 0.0 6 498 14 1 242 561 0.0 6 547 13 0 1 243 393 0.0 6 385 7 1 244 368 0.0 6 355 13 245 480 0.0 6 470 10 246 328 0.0 6 324 4 247 307 0.0 6 297 8 2 248 269 0.0 6 260 7 1 1 249 420 0.0 6 409 11 250 375 0.0 6 364 11 251 455 0.0 6 437 17 1 252 398 0.0 6 391 7 253 323 0.0 6 308 13 1 1 254 446 0.0 6 435 11 255 359 0.0 6 345 14 256 435 0.0 6 429 6 257 399 0.0 6 388 11 258 319 0.0 6 303 14 2 259 644 0.0 6 622 21 1 260 778 0.0 6 761 15 2 261 403 0.0 6 384 19 262 459 0.0 6 435 23 1 263 606 0.0 6 589 16 1 264 465 0.0 6 448 17 265 589 0.0 6 578 11 266 385 0.0 6 371 14 267 412 0.0 6 401 9 2 268 585 0.0 6 556 28 1 269 625 0.0 6 607 17 1 270 320 0.0 6 301 17 2 271 765 0.0 6 732 32 1 272 940 0.0 6 914 25 1 273 759 0.0 6 735 23 1 274 860 0.0 6 835 25 275 866 0.0 6 841 24 0 0 0 0 1 276 1214 0.0 6 1170 41 3 277 1431 0.0 6 1398 32 1 278 1245 0.0 6 1208 37 279 3352 0.0 6 3280 70 2 280 7044 0.0 6 6833 200 9 1 1 281 25227 0.0 6 24784 433 7 1 1 1 282 16 0.0 6 14 2 283 6 0.0 6 5 0 1 284 2 0.0 6 2 286 1 0.0 6 1 288 1 0.0 6 1 289 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 238 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 16 0.0 6 16 70 2 0.0 6 2 71 1 0.0 6 1 72 6 0.0 6 6 74 2 0.0 6 2 75 3 0.0 6 3 76 2 0.0 6 2 77 4 0.0 6 4 78 2 0.0 6 2 79 2 0.0 6 2 81 3 0.0 6 3 85 1 0.0 6 1 86 1 0.0 6 1 87 1 0.0 6 1 88 2 0.0 6 2 89 3 0.0 6 3 90 2 0.0 6 2 93 2 0.0 6 2 94 2 0.0 6 2 95 1 0.0 6 1 96 3 0.0 6 3 97 1 0.0 6 1 98 1 0.0 6 1 99 3 0.0 6 3 100 2 0.0 6 2 101 1 0.0 6 1 102 1 0.0 6 1 105 2 0.0 6 2 107 4 0.0 6 4 108 1 0.0 6 1 111 2 0.0 6 2 112 1 0.0 6 1 113 1 0.0 6 1 116 1 0.0 6 1 118 1 0.0 6 1 120 1 0.0 6 1 121 2 0.0 6 2 124 1 0.0 6 1 125 1 0.0 6 1 126 3 0.0 6 3 128 1 0.0 6 1 129 1 0.0 6 1 131 1 0.0 6 1 132 6 0.0 6 6 133 4 0.0 6 4 134 4 0.0 6 4 135 1 0.0 6 1 138 2 0.0 6 2 139 4 0.0 6 4 140 1 0.0 6 1 143 2 0.0 6 2 144 2 0.0 6 2 148 2 0.0 6 2 150 1 0.0 6 1 152 1 0.0 6 1 153 3 0.0 6 3 155 4 0.0 6 4 156 1 0.0 6 1 158 1 0.0 6 1 159 2 0.0 6 2 160 1 0.0 6 1 161 3 0.0 6 3 165 1 0.0 6 1 166 1 0.0 6 1 167 3 0.0 6 3 168 1 0.0 6 1 169 3 0.0 6 3 170 1 0.0 6 1 173 2 0.0 6 2 175 1 0.0 6 1 176 1 0.0 6 1 178 1 0.0 6 1 181 2 0.0 6 2 182 3 0.0 6 3 183 5 0.0 6 5 184 1 0.0 6 1 186 2 0.0 6 2 189 2 0.0 6 2 190 1 0.0 6 1 191 3 0.0 6 3 192 1 0.0 6 1 193 3 0.0 6 3 194 1 0.0 6 1 195 1 0.0 6 1 199 4 0.0 6 4 201 1 0.0 6 1 202 3 0.0 6 3 204 2 0.0 6 2 206 2 0.0 6 2 207 2 0.0 6 2 208 3 0.0 6 3 210 1 0.0 6 1 211 2 0.0 6 2 212 1 0.0 6 1 213 1 0.0 6 1 218 2 0.0 6 2 219 2 0.0 6 2 221 2 0.0 6 2 222 1 0.0 6 1 224 1 0.0 6 1 225 2 0.0 6 2 226 1 0.0 6 1 227 1 0.0 6 1 228 1 0.0 6 1 230 1 0.0 6 1 231 1 0.0 6 1 232 2 0.0 6 2 235 1 0.0 6 1 237 1 0.0 6 1 239 2 0.0 6 2 240 1 0.0 6 1 242 1 0.0 6 1 243 2 0.0 6 2 244 2 0.0 6 2 245 1 0.0 6 1 247 1 0.0 6 1 248 1 0.0 6 1 249 2 0.0 6 2 251 1 0.0 6 1 262 1 0.0 6 1 265 1 0.0 6 1 294 1 0.0 6 1 300 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 79590 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 2 19.5 1 2 7 1 4.9 2 1 8 2 1.2 2 2 9 2 0.3 2 1 1 11 4 0.0 3 2 2 12 4 0.0 3 2 2 13 20 0.0 3 18 1 0 0 1 14 7 0.0 4 7 16 13 0.0 4 12 1 17 11 0.0 5 9 2 18 15 0.0 5 15 19 20 0.0 5 18 2 20 77 0.0 6 76 1 21 60 0.0 6 58 1 1 22 42 0.0 6 40 2 23 34 0.0 6 34 24 23 0.0 6 22 1 25 19 0.0 6 19 26 16 0.0 6 16 27 12 0.0 6 12 28 13 0.0 6 13 29 10 0.0 6 9 1 30 16 0.0 6 15 1 31 19 0.0 6 17 2 32 10 0.0 6 8 2 33 15 0.0 6 13 2 34 8 0.0 6 8 35 9 0.0 6 9 36 3 0.0 6 3 37 15 0.0 6 14 0 1 38 11 0.0 6 10 1 39 14 0.0 6 12 2 40 10 0.0 6 9 1 41 10 0.0 6 10 42 4 0.0 6 3 1 43 15 0.0 6 15 44 24 0.0 6 22 2 45 13 0.0 6 13 46 19 0.0 6 16 3 47 30 0.0 6 28 2 48 16 0.0 6 14 2 49 19 0.0 6 19 50 16 0.0 6 15 1 51 11 0.0 6 8 3 52 19 0.0 6 18 1 53 15 0.0 6 13 2 54 20 0.0 6 20 55 25 0.0 6 23 1 1 56 26 0.0 6 24 2 57 18 0.0 6 17 1 58 12 0.0 6 11 1 59 25 0.0 6 22 1 0 1 1 60 15 0.0 6 15 61 15 0.0 6 13 2 62 20 0.0 6 17 3 63 12 0.0 6 12 64 24 0.0 6 22 0 1 0 1 65 9 0.0 6 9 66 32 0.0 6 27 4 0 0 1 67 30 0.0 6 28 2 68 35 0.0 6 34 1 69 156 0.0 6 152 2 0 1 1 70 30 0.0 6 29 1 71 96 0.0 6 91 4 1 72 35 0.0 6 33 2 73 24 0.0 6 23 1 74 23 0.0 6 22 1 75 30 0.0 6 29 1 76 37 0.0 6 37 77 26 0.0 6 23 3 78 32 0.0 6 30 1 0 0 0 1 79 24 0.0 6 23 1 80 28 0.0 6 26 2 81 23 0.0 6 20 2 1 82 42 0.0 6 41 1 83 66 0.0 6 59 7 84 58 0.0 6 53 4 0 0 1 85 63 0.0 6 60 3 86 30 0.0 6 29 1 87 59 0.0 6 54 5 88 25 0.0 6 22 3 89 46 0.0 6 39 6 0 0 1 90 43 0.0 6 41 2 91 43 0.0 6 40 2 0 0 1 92 24 0.0 6 22 2 93 68 0.0 6 64 3 0 0 0 1 94 134 0.0 6 123 11 95 63 0.0 6 59 4 96 51 0.0 6 50 1 97 66 0.0 6 64 2 98 56 0.0 6 52 3 0 0 1 99 64 0.0 6 59 4 1 100 66 0.0 6 64 2 101 49 0.0 6 48 1 102 118 0.0 6 109 8 0 0 1 103 89 0.0 6 83 6 104 88 0.0 6 86 2 105 107 0.0 6 97 9 0 0 1 106 101 0.0 6 98 3 107 114 0.0 6 108 3 3 108 61 0.0 6 55 4 1 1 109 79 0.0 6 78 1 110 62 0.0 6 57 5 111 73 0.0 6 68 5 112 69 0.0 6 66 3 113 51 0.0 6 48 3 114 106 0.0 6 103 3 115 81 0.0 6 76 4 0 0 1 116 105 0.0 6 102 3 117 76 0.0 6 74 1 0 0 0 1 118 85 0.0 6 82 3 119 102 0.0 6 95 6 0 0 1 120 170 0.0 6 165 4 0 0 1 121 106 0.0 6 104 1 0 0 1 122 127 0.0 6 123 2 2 123 136 0.0 6 128 5 2 0 0 1 124 150 0.0 6 141 8 1 125 112 0.0 6 103 8 0 0 1 126 89 0.0 6 85 3 1 127 144 0.0 6 140 4 128 120 0.0 6 115 5 129 143 0.0 6 139 4 130 102 0.0 6 102 131 210 0.0 6 202 6 0 1 0 1 132 258 0.0 6 246 11 0 0 1 133 277 0.0 6 268 9 134 292 0.0 6 279 13 135 460 0.0 6 445 14 0 1 136 275 0.0 6 264 11 137 365 0.0 6 354 11 138 246 0.0 6 239 6 0 0 1 139 306 0.0 6 296 9 1 140 375 0.0 6 367 7 0 1 141 395 0.0 6 384 9 1 0 1 142 497 0.0 6 485 11 0 0 1 143 418 0.0 6 406 10 1 0 1 144 588 0.0 6 572 14 1 0 1 145 379 0.0 6 373 6 146 383 0.0 6 373 9 0 0 1 147 266 0.0 6 258 8 148 392 0.0 6 378 13 0 0 0 1 149 522 0.0 6 507 13 0 0 2 150 718 0.0 6 686 29 1 0 2 151 402 0.0 6 388 11 1 0 2 152 1033 0.0 6 997 34 2 153 559 0.0 6 539 20 154 768 0.0 6 749 17 1 1 155 692 0.0 6 676 14 1 0 1 156 543 0.0 6 524 19 157 737 0.0 6 718 18 0 0 1 158 737 0.0 6 723 14 159 468 0.0 6 455 12 0 1 160 759 0.0 6 738 19 0 1 0 0 1 161 507 0.0 6 495 9 2 0 1 162 449 0.0 6 433 15 0 0 0 0 1 163 616 0.0 6 592 23 1 164 542 0.0 6 528 13 0 1 165 637 0.0 6 622 14 0 1 166 743 0.0 6 724 19 167 395 0.0 6 380 13 0 2 168 911 0.0 6 897 14 169 296 0.0 6 284 12 170 544 0.0 6 529 14 1 171 927 0.0 6 900 24 2 0 1 172 330 0.0 6 324 4 2 173 484 0.0 6 468 14 2 174 733 0.0 6 717 16 175 1263 0.0 6 1230 33 176 740 0.0 6 710 29 0 0 1 177 935 0.0 6 913 19 1 0 2 178 775 0.0 6 760 14 0 0 1 179 842 0.0 6 811 30 0 0 1 180 800 0.0 6 779 16 4 0 1 181 953 0.0 6 939 13 1 182 354 0.0 6 337 15 1 1 183 839 0.0 6 820 18 0 0 1 184 286 0.0 6 278 7 0 1 185 580 0.0 6 561 17 1 1 186 1928 0.0 6 1860 65 2 0 1 187 1581 0.0 6 1539 38 2 0 2 188 1635 0.0 6 1594 39 0 1 1 189 1409 0.0 6 1367 41 1 190 2096 0.0 6 2039 56 1 191 1621 0.0 6 1573 46 1 0 1 192 1200 0.0 6 1163 37 193 755 0.0 6 718 31 3 1 2 194 1271 0.0 6 1237 29 2 1 2 195 1830 0.0 6 1774 55 0 0 1 196 2966 0.0 6 2883 77 3 1 2 197 639 0.0 6 606 28 4 0 1 198 2694 0.0 6 2600 90 2 0 2 199 3637 0.0 6 3536 97 2 0 2 200 1110 0.0 6 1077 33 201 1260 0.0 6 1228 31 1 202 730 0.0 6 700 29 1 203 830 0.0 6 803 26 1 204 985 0.0 6 959 26 205 354 0.0 6 344 9 0 0 1 206 323 0.0 6 301 22 207 255 0.0 6 252 2 0 0 0 1 208 453 0.0 6 448 5 209 382 0.0 6 369 12 0 1 210 344 0.0 6 339 5 211 456 0.0 6 436 19 1 212 415 0.0 6 404 9 1 0 1 213 150 0.0 6 142 7 0 0 1 214 471 0.0 6 463 8 215 375 0.0 6 366 9 216 864 0.0 6 841 23 217 579 0.0 6 564 13 0 1 1 218 493 0.0 6 481 12 219 400 0.0 6 389 9 0 0 2 220 335 0.0 6 329 6 221 368 0.0 6 357 9 1 0 0 1 222 451 0.0 6 434 16 1 223 1036 0.0 6 1010 25 1 224 295 0.0 6 283 11 0 0 1 225 490 0.0 6 476 14 226 346 0.0 6 335 10 0 1 227 239 0.0 6 233 6 228 181 0.0 6 175 6 229 283 0.0 6 276 6 1 230 190 0.0 6 184 5 1 231 216 0.0 6 206 10 232 273 0.0 6 259 14 233 346 0.0 6 333 12 1 234 183 0.0 6 178 4 0 0 1 235 76 0.0 6 74 2 236 42 0.0 6 40 2 237 44 0.0 6 43 1 238 21 0.0 6 17 4 239 20 0.0 6 20 240 117 0.0 6 113 4 241 83 0.0 6 81 1 0 0 1 242 175 0.0 6 171 4 243 161 0.0 6 156 5 244 102 0.0 6 100 2 245 58 0.0 6 57 1 246 31 0.0 6 30 1 247 25 0.0 6 24 1 248 38 0.0 6 37 1 249 20 0.0 6 20 250 24 0.0 6 24 251 14 0.0 6 12 2 252 4 0.0 6 4 253 23 0.0 6 23 254 6 0.0 6 6 255 5 0.0 6 5 256 2 0.0 6 2 259 1 0.0 6 1 260 1 0.0 6 1 261 1 0.0 6 1 279 13 0.0 6 13 280 43 0.0 6 41 2 281 204 0.0 6 200 3 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 211 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 1 0.0 6 1 71 1 0.0 6 1 72 3 0.0 6 3 73 8 0.0 6 8 74 2 0.0 6 2 75 6 0.0 6 6 76 4 0.0 6 4 77 4 0.0 6 4 78 1 0.0 6 1 79 3 0.0 6 3 80 4 0.0 6 4 81 3 0.0 6 3 82 1 0.0 6 1 83 1 0.0 6 1 84 3 0.0 6 3 85 2 0.0 6 2 87 3 0.0 6 3 88 3 0.0 6 3 89 2 0.0 6 2 90 2 0.0 6 2 92 2 0.0 6 2 96 1 0.0 6 1 98 1 0.0 6 1 99 3 0.0 6 3 101 2 0.0 6 2 103 2 0.0 6 2 104 1 0.0 6 1 106 1 0.0 6 1 107 1 0.0 6 1 108 6 0.0 6 6 110 1 0.0 6 1 111 1 0.0 6 1 112 1 0.0 6 1 113 3 0.0 6 3 115 1 0.0 6 1 116 2 0.0 6 2 118 2 0.0 6 2 119 2 0.0 6 2 120 2 0.0 6 2 123 2 0.0 6 2 124 2 0.0 6 2 126 3 0.0 6 3 127 1 0.0 6 1 128 1 0.0 6 1 129 2 0.0 6 2 130 1 0.0 6 1 131 2 0.0 6 2 132 5 0.0 6 5 133 4 0.0 6 4 134 2 0.0 6 2 135 3 0.0 6 3 136 1 0.0 6 1 137 2 0.0 6 2 138 1 0.0 6 1 139 1 0.0 6 1 140 1 0.0 6 1 141 1 0.0 6 1 142 2 0.0 6 2 143 1 0.0 6 1 144 2 0.0 6 2 145 1 0.0 6 1 148 1 0.0 6 1 149 2 0.0 6 2 150 3 0.0 6 3 151 1 0.0 6 1 152 2 0.0 6 2 153 1 0.0 6 1 154 1 0.0 6 1 156 2 0.0 6 2 157 3 0.0 6 3 158 2 0.0 6 2 159 1 0.0 6 1 161 1 0.0 6 1 162 1 0.0 6 1 164 1 0.0 6 1 165 2 0.0 6 2 167 1 0.0 6 1 168 1 0.0 6 1 169 1 0.0 6 1 170 3 0.0 6 3 171 1 0.0 6 1 172 2 0.0 6 2 173 2 0.0 6 2 174 2 0.0 6 2 175 2 0.0 6 2 176 2 0.0 6 2 177 3 0.0 6 3 178 1 0.0 6 1 179 1 0.0 6 1 180 1 0.0 6 1 182 1 0.0 6 1 183 1 0.0 6 1 184 3 0.0 6 3 187 1 0.0 6 1 188 2 0.0 6 2 190 1 0.0 6 1 192 3 0.0 6 3 194 2 0.0 6 2 195 3 0.0 6 3 196 2 0.0 6 2 201 2 0.0 6 2 202 1 0.0 6 1 206 1 0.0 6 1 209 2 0.0 6 2 211 1 0.0 6 1 220 1 0.0 6 1 223 1 0.0 6 1 229 1 0.0 6 1 235 1 0.0 6 1 243 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_06_65_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_06_292_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_06_65_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_06_292_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.47 s (30 µs/read; 2.02 M reads/minute). === Summary === Total read pairs processed: 83,102 Read 1 with adapter: 83,100 (100.0%) Read 2 with adapter: 83,070 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 34 (0.0%) Pairs written (passing filters): 83,068 (100.0%) Total basepairs processed: 41,479,170 bp Read 1: 23,660,555 bp Read 2: 17,818,615 bp Total written (filtered): 4,902,743 bp (11.8%) Read 1: 2,492,931 bp Read 2: 2,409,812 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 83076 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1298.5 0 1 1 4 1 324.6 1 0 1 6 2 20.3 1 0 0 2 7 1 5.1 2 0 0 1 9 2 0.3 2 1 1 15 2 0.0 4 2 16 2 0.0 4 2 17 1 0.0 5 0 1 19 1 0.0 5 1 20 21 0.0 6 18 3 21 11 0.0 6 9 1 0 1 22 5 0.0 6 4 1 23 2 0.0 6 2 24 1 0.0 6 1 25 9 0.0 6 9 26 7 0.0 6 7 27 4 0.0 6 4 28 4 0.0 6 3 1 30 2 0.0 6 1 1 31 3 0.0 6 3 33 2 0.0 6 2 34 4 0.0 6 4 35 4 0.0 6 4 37 3 0.0 6 3 38 3 0.0 6 3 39 1 0.0 6 1 40 4 0.0 6 3 0 0 1 41 4 0.0 6 4 42 2 0.0 6 2 43 2 0.0 6 2 44 3 0.0 6 3 45 3 0.0 6 3 46 3 0.0 6 3 47 2 0.0 6 2 49 4 0.0 6 4 50 1 0.0 6 1 51 2 0.0 6 2 52 3 0.0 6 1 1 0 1 53 4 0.0 6 4 54 6 0.0 6 6 56 3 0.0 6 3 57 3 0.0 6 2 1 58 5 0.0 6 4 0 1 59 2 0.0 6 2 60 2 0.0 6 2 61 4 0.0 6 3 1 62 3 0.0 6 3 63 1 0.0 6 1 64 3 0.0 6 2 1 65 1 0.0 6 1 66 3 0.0 6 3 68 3 0.0 6 3 70 2 0.0 6 2 73 6 0.0 6 6 76 1 0.0 6 1 77 5 0.0 6 5 78 5 0.0 6 5 81 1 0.0 6 1 83 2 0.0 6 2 84 5 0.0 6 4 1 85 1 0.0 6 1 86 13 0.0 6 11 2 87 6 0.0 6 5 1 89 9 0.0 6 8 1 90 4 0.0 6 4 91 1 0.0 6 1 92 5 0.0 6 5 93 2 0.0 6 1 1 94 1 0.0 6 1 96 1 0.0 6 1 97 3 0.0 6 3 98 8 0.0 6 7 1 99 1 0.0 6 1 100 4 0.0 6 4 101 6 0.0 6 6 102 3 0.0 6 2 1 103 4 0.0 6 4 104 3 0.0 6 2 1 105 5 0.0 6 5 106 5 0.0 6 5 107 5 0.0 6 5 108 14 0.0 6 13 1 109 9 0.0 6 8 1 110 6 0.0 6 5 1 111 6 0.0 6 6 112 9 0.0 6 8 1 113 5 0.0 6 5 114 3 0.0 6 3 115 9 0.0 6 8 1 116 4 0.0 6 4 117 9 0.0 6 9 118 4 0.0 6 3 1 119 11 0.0 6 11 120 4 0.0 6 4 121 14 0.0 6 14 122 26 0.0 6 26 123 16 0.0 6 15 1 124 7 0.0 6 7 125 20 0.0 6 19 1 126 95 0.0 6 93 2 127 74 0.0 6 73 1 128 33 0.0 6 31 2 129 6 0.0 6 6 130 10 0.0 6 9 0 1 131 33 0.0 6 30 3 132 32 0.0 6 31 1 133 19 0.0 6 17 2 134 13 0.0 6 12 1 135 20 0.0 6 20 136 26 0.0 6 25 1 137 14 0.0 6 14 138 12 0.0 6 12 139 15 0.0 6 15 140 27 0.0 6 26 1 141 39 0.0 6 39 142 55 0.0 6 55 143 46 0.0 6 46 144 46 0.0 6 46 145 36 0.0 6 36 146 48 0.0 6 48 147 48 0.0 6 45 3 148 54 0.0 6 52 2 149 25 0.0 6 23 1 0 0 1 150 38 0.0 6 37 0 1 151 49 0.0 6 49 152 26 0.0 6 25 1 153 26 0.0 6 26 154 40 0.0 6 38 2 155 31 0.0 6 30 1 156 61 0.0 6 59 2 157 29 0.0 6 28 1 158 29 0.0 6 28 1 159 23 0.0 6 22 1 160 29 0.0 6 29 161 49 0.0 6 47 2 162 25 0.0 6 24 1 163 65 0.0 6 58 7 164 32 0.0 6 32 165 30 0.0 6 30 166 30 0.0 6 30 167 28 0.0 6 28 168 23 0.0 6 23 169 32 0.0 6 31 1 170 52 0.0 6 52 171 78 0.0 6 76 2 172 35 0.0 6 34 1 173 113 0.0 6 110 3 174 106 0.0 6 103 3 175 106 0.0 6 101 5 176 74 0.0 6 70 4 177 69 0.0 6 64 4 1 178 77 0.0 6 74 3 179 66 0.0 6 65 1 180 104 0.0 6 103 1 181 96 0.0 6 95 1 182 139 0.0 6 136 3 183 141 0.0 6 137 4 184 180 0.0 6 172 7 1 185 141 0.0 6 137 4 186 84 0.0 6 82 2 187 96 0.0 6 94 1 1 188 131 0.0 6 128 3 189 194 0.0 6 188 6 190 87 0.0 6 81 5 1 191 168 0.0 6 167 1 192 127 0.0 6 122 5 193 121 0.0 6 119 1 1 194 160 0.0 6 158 2 195 129 0.0 6 121 8 196 185 0.0 6 182 3 197 158 0.0 6 151 7 198 197 0.0 6 195 2 199 219 0.0 6 214 5 200 215 0.0 6 205 10 201 288 0.0 6 283 4 1 202 239 0.0 6 229 10 203 281 0.0 6 276 5 204 166 0.0 6 158 8 205 267 0.0 6 260 7 206 208 0.0 6 206 1 1 207 267 0.0 6 257 9 1 208 188 0.0 6 183 5 209 160 0.0 6 157 3 210 176 0.0 6 170 6 211 194 0.0 6 191 2 1 212 148 0.0 6 145 2 0 1 213 162 0.0 6 154 7 0 0 1 214 227 0.0 6 221 6 215 487 0.0 6 481 6 216 265 0.0 6 259 6 217 391 0.0 6 384 7 218 239 0.0 6 231 6 2 219 316 0.0 6 309 7 220 481 0.0 6 469 11 1 221 232 0.0 6 230 2 222 236 0.0 6 229 5 0 2 223 355 0.0 6 349 6 224 709 0.0 6 694 12 2 1 225 692 0.0 6 677 15 226 558 0.0 6 544 13 1 227 336 0.0 6 325 11 228 409 0.0 6 405 4 229 383 0.0 6 370 13 230 537 0.0 6 522 15 231 853 0.0 6 838 15 232 497 0.0 6 486 11 233 402 0.0 6 392 10 234 460 0.0 6 449 10 1 235 375 0.0 6 362 13 236 587 0.0 6 572 14 0 1 237 376 0.0 6 363 13 238 680 0.0 6 662 18 239 815 0.0 6 799 16 240 841 0.0 6 826 15 241 644 0.0 6 628 16 242 516 0.0 6 504 12 243 1075 0.0 6 1060 15 244 409 0.0 6 396 12 1 245 633 0.0 6 619 11 3 246 333 0.0 6 322 11 247 806 0.0 6 785 21 248 399 0.0 6 393 6 249 540 0.0 6 529 11 250 503 0.0 6 492 11 251 385 0.0 6 368 17 252 972 0.0 6 951 19 2 253 498 0.0 6 486 11 1 254 434 0.0 6 424 10 255 414 0.0 6 409 5 256 509 0.0 6 492 17 257 1151 0.0 6 1129 22 258 1175 0.0 6 1161 14 259 378 0.0 6 365 13 260 779 0.0 6 758 20 1 261 1907 0.0 6 1868 37 2 262 947 0.0 6 926 20 1 263 719 0.0 6 707 12 264 284 0.0 6 280 3 1 265 639 0.0 6 626 13 266 606 0.0 6 592 14 267 1151 0.0 6 1128 21 1 1 268 285 0.0 6 268 12 4 0 1 269 859 0.0 6 834 25 270 3949 0.0 6 3876 72 1 271 883 0.0 6 855 27 1 272 1700 0.0 6 1667 33 273 2012 0.0 6 1972 40 274 1987 0.0 6 1953 32 1 0 0 1 275 2328 0.0 6 2282 45 1 276 900 0.0 6 881 16 3 277 1691 0.0 6 1644 45 1 1 278 2423 0.0 6 2377 44 2 279 1505 0.0 6 1418 85 2 280 20850 0.0 6 20382 462 6 281 1510 0.0 6 1394 116 282 57 0.0 6 49 6 1 1 283 4 0.0 6 3 0 0 0 1 284 4 0.0 6 4 286 1 0.0 6 1 288 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 24 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 1 0.0 6 1 72 1 0.0 6 1 73 1 0.0 6 1 74 1 0.0 6 1 76 1 0.0 6 1 77 1 0.0 6 1 96 1 0.0 6 1 101 1 0.0 6 1 102 1 0.0 6 1 107 1 0.0 6 1 108 1 0.0 6 1 113 1 0.0 6 1 122 1 0.0 6 1 124 1 0.0 6 1 132 1 0.0 6 1 134 1 0.0 6 1 136 1 0.0 6 1 137 1 0.0 6 1 161 1 0.0 6 1 174 1 0.0 6 1 196 1 0.0 6 1 216 1 0.0 6 1 248 1 0.0 6 1 268 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 83047 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 324.6 1 0 1 7 2 5.1 2 1 1 8 1 1.3 2 1 9 1 0.3 2 1 11 3 0.0 3 2 0 1 12 6 0.0 3 6 13 9 0.0 3 6 1 1 1 14 6 0.0 4 5 0 0 0 1 15 6 0.0 4 5 1 16 3 0.0 4 3 17 18 0.0 5 18 18 5 0.0 5 2 3 19 36 0.0 5 34 2 20 85 0.0 6 81 4 21 75 0.0 6 74 1 22 47 0.0 6 45 2 23 33 0.0 6 30 3 24 22 0.0 6 20 2 25 17 0.0 6 15 1 1 26 15 0.0 6 15 27 12 0.0 6 12 28 14 0.0 6 13 1 29 19 0.0 6 18 0 1 30 15 0.0 6 14 1 31 9 0.0 6 9 32 13 0.0 6 12 1 33 19 0.0 6 18 1 34 20 0.0 6 18 1 1 35 10 0.0 6 9 1 36 9 0.0 6 7 2 37 14 0.0 6 12 2 38 21 0.0 6 19 2 39 10 0.0 6 10 40 11 0.0 6 10 1 41 5 0.0 6 4 1 42 6 0.0 6 6 43 9 0.0 6 9 44 14 0.0 6 11 3 45 12 0.0 6 8 4 46 25 0.0 6 23 2 47 12 0.0 6 11 0 0 0 1 48 15 0.0 6 14 1 49 21 0.0 6 17 4 50 24 0.0 6 23 1 51 30 0.0 6 22 8 52 23 0.0 6 20 2 0 0 1 53 20 0.0 6 18 2 54 15 0.0 6 14 1 55 28 0.0 6 25 3 56 27 0.0 6 24 2 1 57 31 0.0 6 30 1 58 17 0.0 6 17 59 18 0.0 6 18 60 27 0.0 6 23 3 0 0 1 61 18 0.0 6 16 2 62 13 0.0 6 12 0 1 63 15 0.0 6 14 1 64 10 0.0 6 9 1 65 8 0.0 6 8 66 12 0.0 6 12 67 10 0.0 6 8 2 68 23 0.0 6 15 7 1 69 31 0.0 6 29 2 70 165 0.0 6 156 9 71 34 0.0 6 30 4 72 103 0.0 6 97 5 1 73 16 0.0 6 15 1 74 31 0.0 6 31 75 26 0.0 6 23 2 1 76 18 0.0 6 18 77 25 0.0 6 23 2 78 35 0.0 6 29 4 2 79 14 0.0 6 14 80 25 0.0 6 21 3 0 0 1 81 14 0.0 6 13 0 0 0 1 82 26 0.0 6 23 2 1 83 49 0.0 6 44 5 84 64 0.0 6 57 6 1 85 59 0.0 6 57 1 0 0 1 86 68 0.0 6 61 6 0 0 1 87 73 0.0 6 64 9 88 25 0.0 6 21 4 89 29 0.0 6 28 1 90 51 0.0 6 47 4 91 38 0.0 6 36 0 2 92 39 0.0 6 36 3 93 44 0.0 6 41 3 94 90 0.0 6 82 7 1 95 41 0.0 6 36 4 0 0 0 1 96 64 0.0 6 60 4 97 43 0.0 6 41 1 0 0 1 98 46 0.0 6 43 1 1 0 1 99 87 0.0 6 77 10 100 56 0.0 6 54 1 1 101 58 0.0 6 49 9 102 95 0.0 6 86 8 0 1 103 109 0.0 6 103 5 1 104 86 0.0 6 79 7 105 74 0.0 6 67 6 1 106 52 0.0 6 46 5 1 107 92 0.0 6 90 2 108 37 0.0 6 36 1 109 43 0.0 6 41 2 110 57 0.0 6 57 111 43 0.0 6 35 8 112 51 0.0 6 46 4 0 0 1 113 50 0.0 6 46 4 114 54 0.0 6 51 3 115 65 0.0 6 61 4 116 49 0.0 6 46 3 117 42 0.0 6 38 3 0 0 1 118 62 0.0 6 58 4 119 82 0.0 6 74 8 120 124 0.0 6 118 6 121 70 0.0 6 66 3 0 0 1 122 119 0.0 6 109 9 0 0 1 123 132 0.0 6 126 6 124 164 0.0 6 154 9 0 0 1 125 67 0.0 6 64 3 126 57 0.0 6 51 6 127 80 0.0 6 79 1 128 97 0.0 6 88 6 0 0 2 1 129 87 0.0 6 83 3 1 130 92 0.0 6 86 6 131 130 0.0 6 119 10 1 132 251 0.0 6 238 11 1 0 1 133 200 0.0 6 188 12 134 251 0.0 6 238 11 2 135 322 0.0 6 309 12 0 0 0 1 136 179 0.0 6 168 10 0 0 0 0 1 137 236 0.0 6 225 11 138 217 0.0 6 210 6 1 139 175 0.0 6 171 3 0 1 140 173 0.0 6 163 6 3 1 141 254 0.0 6 247 6 0 1 142 365 0.0 6 352 13 143 280 0.0 6 269 11 144 486 0.0 6 467 17 2 145 232 0.0 6 224 6 0 0 2 146 248 0.0 6 245 3 147 139 0.0 6 131 8 148 223 0.0 6 219 4 149 353 0.0 6 336 15 1 0 1 150 495 0.0 6 477 15 2 0 1 151 260 0.0 6 241 17 2 152 651 0.0 6 626 24 1 153 617 0.0 6 599 16 2 154 580 0.0 6 558 22 155 679 0.0 6 653 24 1 0 1 156 530 0.0 6 509 16 4 1 157 627 0.0 6 604 21 1 0 1 158 616 0.0 6 598 18 159 281 0.0 6 267 14 160 671 0.0 6 641 30 161 406 0.0 6 396 10 162 370 0.0 6 360 10 163 548 0.0 6 529 16 2 0 0 1 164 449 0.0 6 441 8 165 528 0.0 6 510 17 0 0 1 166 710 0.0 6 690 20 167 301 0.0 6 292 9 168 802 0.0 6 779 22 0 0 1 169 240 0.0 6 237 3 170 292 0.0 6 273 18 1 171 643 0.0 6 617 26 172 227 0.0 6 221 5 1 173 335 0.0 6 319 14 0 0 2 174 522 0.0 6 504 17 1 175 1003 0.0 6 965 37 0 0 1 176 669 0.0 6 645 24 177 816 0.0 6 786 24 2 3 1 178 773 0.0 6 754 18 1 179 827 0.0 6 789 38 180 818 0.0 6 795 23 181 1168 0.0 6 1137 29 1 0 1 182 429 0.0 6 408 21 183 896 0.0 6 875 19 1 0 1 184 155 0.0 6 143 11 1 185 266 0.0 6 248 17 1 186 1094 0.0 6 1046 45 1 0 2 187 1680 0.0 6 1622 54 1 1 1 0 1 188 1947 0.0 6 1903 43 1 189 1388 0.0 6 1344 43 1 190 2274 0.0 6 2222 48 2 1 1 191 1766 0.0 6 1721 40 4 1 192 1293 0.0 6 1258 33 0 0 1 1 193 945 0.0 6 914 30 0 1 194 1609 0.0 6 1559 50 195 1863 0.0 6 1807 56 196 3385 0.0 6 3280 98 3 0 3 0 1 197 812 0.0 6 787 21 3 0 1 198 1415 0.0 6 1365 49 1 199 4224 0.0 6 4119 103 1 1 200 1783 0.0 6 1733 48 1 0 1 201 2011 0.0 6 1955 52 1 2 1 202 1571 0.0 6 1533 35 1 1 1 203 1053 0.0 6 1026 26 0 0 1 204 980 0.0 6 950 30 205 426 0.0 6 412 13 0 1 206 529 0.0 6 512 14 2 0 1 207 289 0.0 6 283 6 208 617 0.0 6 601 15 0 1 209 569 0.0 6 556 12 0 0 0 1 210 514 0.0 6 494 16 2 1 0 1 211 690 0.0 6 672 16 1 1 212 908 0.0 6 886 21 0 0 1 213 217 0.0 6 208 6 3 214 493 0.0 6 477 15 1 215 327 0.0 6 316 11 216 1694 0.0 6 1647 44 1 0 2 217 1453 0.0 6 1411 41 0 0 1 218 773 0.0 6 750 22 0 0 1 219 638 0.0 6 608 28 1 0 1 220 798 0.0 6 775 21 0 0 2 221 1810 0.0 6 1765 44 0 0 1 222 1179 0.0 6 1141 36 2 223 1330 0.0 6 1302 26 1 1 224 128 0.0 6 123 3 1 1 225 403 0.0 6 387 15 1 226 234 0.0 6 220 14 227 331 0.0 6 327 4 228 210 0.0 6 203 6 1 229 330 0.0 6 323 6 0 0 1 230 127 0.0 6 124 3 231 224 0.0 6 219 4 0 1 232 295 0.0 6 287 7 0 0 1 233 369 0.0 6 349 20 234 130 0.0 6 126 4 235 166 0.0 6 163 3 236 53 0.0 6 51 2 237 92 0.0 6 90 2 238 35 0.0 6 34 1 239 38 0.0 6 37 1 240 102 0.0 6 96 6 241 208 0.0 6 200 8 242 167 0.0 6 163 4 243 140 0.0 6 132 8 244 430 0.0 6 423 7 245 127 0.0 6 124 3 246 144 0.0 6 142 2 247 25 0.0 6 25 248 44 0.0 6 43 1 249 50 0.0 6 48 2 250 23 0.0 6 23 251 80 0.0 6 79 0 1 252 9 0.0 6 9 253 20 0.0 6 20 254 63 0.0 6 62 1 255 11 0.0 6 11 256 4 0.0 6 4 257 9 0.0 6 9 258 5 0.0 6 5 259 2 0.0 6 2 260 2 0.0 6 2 262 2 0.0 6 2 263 1 0.0 6 1 266 2 0.0 6 2 280 8 0.0 6 6 2 281 18 0.0 6 17 1 282 31 0.0 6 30 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 23 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 2 0.0 6 2 72 1 0.0 6 1 73 1 0.0 6 1 76 1 0.0 6 1 78 1 0.0 6 1 79 2 0.0 6 2 80 1 0.0 6 1 84 1 0.0 6 1 86 1 0.0 6 1 92 1 0.0 6 1 97 1 0.0 6 1 101 1 0.0 6 1 104 1 0.0 6 1 109 1 0.0 6 1 119 1 0.0 6 1 127 1 0.0 6 1 134 1 0.0 6 1 167 1 0.0 6 1 182 1 0.0 6 1 183 1 0.0 6 1 204 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_15_66_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_15_293_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_15_66_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_15_293_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.79 s (18 µs/read; 3.30 M reads/minute). === Summary === Total read pairs processed: 153,525 Read 1 with adapter: 153,512 (100.0%) Read 2 with adapter: 153,435 (99.9%) == Read fate breakdown == Pairs that were too short: 58 (0.0%) Pairs discarded as untrimmed: 103 (0.1%) Pairs written (passing filters): 153,364 (99.9%) Total basepairs processed: 63,841,044 bp Read 1: 34,661,341 bp Read 2: 29,179,703 bp Total written (filtered): 10,051,228 bp (15.7%) Read 1: 4,913,143 bp Read 2: 5,138,085 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 148350 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 7 2398.8 0 3 4 4 6 599.7 1 3 3 5 2 149.9 1 2 6 3 37.5 1 1 0 2 7 5 9.4 2 5 9 9 0.6 2 6 2 0 1 10 5 0.1 3 5 11 4 0.0 3 4 15 2 0.0 4 2 16 2 0.0 4 1 1 17 7 0.0 5 5 1 1 18 2 0.0 5 2 19 7 0.0 5 3 3 0 1 20 386 0.0 6 370 16 21 223 0.0 6 212 11 22 186 0.0 6 178 8 23 117 0.0 6 114 3 24 102 0.0 6 100 1 0 0 1 25 71 0.0 6 67 4 26 74 0.0 6 73 1 27 42 0.0 6 40 1 0 0 0 1 28 38 0.0 6 36 2 29 32 0.0 6 32 30 29 0.0 6 28 1 31 14 0.0 6 14 32 15 0.0 6 14 0 0 1 33 16 0.0 6 16 34 25 0.0 6 24 1 35 11 0.0 6 9 1 1 36 14 0.0 6 13 1 37 16 0.0 6 16 38 13 0.0 6 11 2 39 19 0.0 6 17 2 40 13 0.0 6 13 41 14 0.0 6 12 2 42 20 0.0 6 18 2 43 16 0.0 6 16 44 9 0.0 6 9 45 21 0.0 6 18 3 46 8 0.0 6 7 1 47 12 0.0 6 12 48 16 0.0 6 16 49 14 0.0 6 14 50 11 0.0 6 11 51 22 0.0 6 22 52 24 0.0 6 24 53 25 0.0 6 23 1 0 0 1 54 13 0.0 6 12 1 55 14 0.0 6 14 56 16 0.0 6 13 1 1 0 0 0 1 57 8 0.0 6 8 58 7 0.0 6 7 59 7 0.0 6 6 0 1 60 18 0.0 6 17 1 61 5 0.0 6 5 62 12 0.0 6 12 63 15 0.0 6 15 64 13 0.0 6 13 65 15 0.0 6 15 66 14 0.0 6 14 67 10 0.0 6 9 1 68 8 0.0 6 5 3 69 14 0.0 6 12 2 70 19 0.0 6 18 0 1 71 17 0.0 6 14 3 72 23 0.0 6 23 73 11 0.0 6 10 1 74 36 0.0 6 36 75 20 0.0 6 19 0 1 76 21 0.0 6 20 1 77 25 0.0 6 22 3 78 17 0.0 6 16 1 79 14 0.0 6 14 80 24 0.0 6 22 2 81 18 0.0 6 17 1 82 25 0.0 6 25 83 17 0.0 6 17 84 23 0.0 6 23 85 23 0.0 6 21 2 86 34 0.0 6 34 87 27 0.0 6 25 2 88 21 0.0 6 21 89 17 0.0 6 17 90 16 0.0 6 16 91 26 0.0 6 24 2 92 20 0.0 6 20 93 18 0.0 6 18 94 14 0.0 6 14 95 14 0.0 6 14 96 14 0.0 6 13 1 97 24 0.0 6 24 98 18 0.0 6 18 99 13 0.0 6 13 100 20 0.0 6 19 1 101 25 0.0 6 25 102 30 0.0 6 29 1 103 28 0.0 6 28 104 32 0.0 6 32 105 33 0.0 6 33 106 42 0.0 6 40 1 0 0 0 1 107 31 0.0 6 31 108 32 0.0 6 30 1 0 0 1 109 37 0.0 6 36 1 110 38 0.0 6 38 111 30 0.0 6 30 112 56 0.0 6 55 0 0 0 1 113 36 0.0 6 36 114 68 0.0 6 67 1 115 48 0.0 6 48 116 51 0.0 6 51 117 63 0.0 6 63 118 51 0.0 6 49 2 119 55 0.0 6 53 2 120 58 0.0 6 56 1 0 0 0 1 121 53 0.0 6 51 1 0 0 0 1 122 53 0.0 6 52 0 1 123 40 0.0 6 39 1 124 57 0.0 6 56 1 125 66 0.0 6 66 126 77 0.0 6 77 127 79 0.0 6 79 128 63 0.0 6 62 1 129 58 0.0 6 57 1 130 95 0.0 6 95 131 75 0.0 6 75 132 95 0.0 6 95 133 75 0.0 6 74 1 134 99 0.0 6 98 0 0 0 0 1 135 114 0.0 6 112 2 136 96 0.0 6 95 1 137 86 0.0 6 86 138 93 0.0 6 93 139 107 0.0 6 107 140 124 0.0 6 123 1 141 129 0.0 6 129 142 227 0.0 6 227 143 412 0.0 6 412 144 559 0.0 6 554 4 0 1 145 768 0.0 6 765 3 146 948 0.0 6 947 1 147 934 0.0 6 930 4 148 1647 0.0 6 1645 2 149 1459 0.0 6 1456 3 150 1810 0.0 6 1806 3 1 151 1845 0.0 6 1841 2 1 0 0 0 1 152 1763 0.0 6 1759 1 0 0 0 3 153 2300 0.0 6 2295 1 0 0 0 4 154 1548 0.0 6 1537 10 0 0 0 1 155 1419 0.0 6 1405 11 1 0 0 0 2 156 1474 0.0 6 1470 4 157 987 0.0 6 985 2 158 612 0.0 6 610 1 1 159 1108 0.0 6 1105 2 1 160 1112 0.0 6 1111 1 161 1094 0.0 6 1093 1 162 1249 0.0 6 1242 7 163 1062 0.0 6 1057 5 164 1251 0.0 6 1249 2 165 1279 0.0 6 1277 1 0 1 166 1158 0.0 6 1156 2 167 1233 0.0 6 1232 1 168 1344 0.0 6 1341 3 169 1352 0.0 6 1347 3 2 170 1140 0.0 6 1139 0 0 1 171 1328 0.0 6 1325 1 1 0 1 172 1260 0.0 6 1258 1 0 0 1 173 1397 0.0 6 1394 3 174 1462 0.0 6 1460 1 1 175 1153 0.0 6 1152 1 176 1031 0.0 6 1028 2 0 0 0 0 1 177 1397 0.0 6 1392 4 1 178 1224 0.0 6 1220 4 179 715 0.0 6 713 2 180 668 0.0 6 666 1 1 181 1394 0.0 6 1389 4 0 0 0 1 182 801 0.0 6 799 2 183 1040 0.0 6 1038 2 184 944 0.0 6 942 2 185 1074 0.0 6 1071 3 186 1200 0.0 6 1198 2 187 1343 0.0 6 1341 2 188 1105 0.0 6 1100 5 189 1319 0.0 6 1317 2 190 1020 0.0 6 1018 2 191 797 0.0 6 793 4 192 833 0.0 6 831 1 0 0 0 0 1 193 702 0.0 6 699 3 194 992 0.0 6 989 3 195 1304 0.0 6 1301 2 1 196 1250 0.0 6 1249 1 197 1701 0.0 6 1695 4 0 1 0 1 198 2077 0.0 6 2070 5 1 0 0 1 199 1696 0.0 6 1691 3 1 0 0 0 1 200 2039 0.0 6 2033 5 0 1 201 1712 0.0 6 1703 9 202 1449 0.0 6 1445 3 0 0 0 0 1 203 1584 0.0 6 1582 1 1 204 1513 0.0 6 1504 8 0 0 0 0 1 205 2480 0.0 6 2476 3 0 0 0 0 1 206 1575 0.0 6 1570 3 1 0 1 207 1914 0.0 6 1912 1 0 0 1 208 2088 0.0 6 2085 2 0 0 1 209 1512 0.0 6 1506 5 0 0 1 210 1759 0.0 6 1751 7 0 0 0 0 1 211 1466 0.0 6 1461 4 0 0 0 0 1 212 1336 0.0 6 1331 3 1 0 0 1 213 1331 0.0 6 1325 6 214 1613 0.0 6 1607 5 0 0 1 215 1637 0.0 6 1631 5 0 0 0 1 216 1720 0.0 6 1716 4 217 1836 0.0 6 1829 7 218 1562 0.0 6 1556 5 1 219 1533 0.0 6 1527 4 0 0 0 1 1 220 1562 0.0 6 1557 3 1 1 221 1351 0.0 6 1347 2 0 0 0 1 1 222 1163 0.0 6 1159 3 1 223 1242 0.0 6 1239 2 0 0 0 1 224 1275 0.0 6 1273 2 225 1054 0.0 6 1049 4 1 226 1194 0.0 6 1192 2 227 1496 0.0 6 1489 6 0 1 228 1388 0.0 6 1381 5 0 1 0 1 229 1155 0.0 6 1152 3 230 948 0.0 6 947 0 1 231 925 0.0 6 921 3 0 0 0 1 232 1066 0.0 6 1062 4 233 951 0.0 6 948 2 1 234 1180 0.0 6 1176 4 235 949 0.0 6 945 3 1 236 888 0.0 6 887 1 237 845 0.0 6 843 2 238 857 0.0 6 852 4 1 239 869 0.0 6 866 3 240 856 0.0 6 853 2 0 0 0 1 241 853 0.0 6 851 2 242 835 0.0 6 833 2 243 723 0.0 6 719 3 0 0 1 244 734 0.0 6 730 4 245 611 0.0 6 607 3 0 0 1 246 505 0.0 6 503 2 247 527 0.0 6 527 248 512 0.0 6 511 1 249 502 0.0 6 500 0 0 0 1 1 250 476 0.0 6 476 251 452 0.0 6 452 252 383 0.0 6 379 4 253 333 0.0 6 331 2 254 301 0.0 6 298 3 255 344 0.0 6 343 1 256 318 0.0 6 317 1 257 288 0.0 6 285 1 1 0 1 258 284 0.0 6 284 259 284 0.0 6 284 260 308 0.0 6 305 3 261 356 0.0 6 353 3 262 274 0.0 6 274 263 222 0.0 6 222 264 205 0.0 6 205 265 217 0.0 6 217 266 189 0.0 6 189 267 185 0.0 6 184 1 268 202 0.0 6 202 269 206 0.0 6 206 270 157 0.0 6 156 1 271 177 0.0 6 177 272 169 0.0 6 167 2 273 166 0.0 6 163 3 274 154 0.0 6 154 275 177 0.0 6 177 276 198 0.0 6 197 1 277 209 0.0 6 208 1 278 278 0.0 6 273 5 279 363 0.0 6 360 3 280 1164 0.0 6 1155 7 0 0 0 1 1 281 2171 0.0 6 2165 5 1 282 14 0.0 6 14 283 3 0.0 6 3 284 1 0.0 6 1 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 5162 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 52 1 0.0 6 0 1 59 32 0.0 6 30 2 61 72 0.0 6 70 2 63 3 0.0 6 3 69 3 0.0 6 3 70 15 0.0 6 15 71 17 0.0 6 17 72 30 0.0 6 30 73 37 0.0 6 37 74 44 0.0 6 43 1 75 55 0.0 6 55 76 48 0.0 6 47 1 77 68 0.0 6 68 78 68 0.0 6 68 79 74 0.0 6 74 80 245 0.0 6 244 1 81 58 0.0 6 58 82 45 0.0 6 44 1 83 53 0.0 6 53 84 25 0.0 6 25 85 31 0.0 6 31 86 43 0.0 6 42 0 0 0 0 0 1 87 39 0.0 6 38 1 88 47 0.0 6 46 1 89 35 0.0 6 35 90 30 0.0 6 30 91 55 0.0 6 55 92 37 0.0 6 37 93 37 0.0 6 37 94 41 0.0 6 41 95 55 0.0 6 55 96 55 0.0 6 54 1 97 52 0.0 6 52 98 46 0.0 6 46 99 52 0.0 6 52 100 35 0.0 6 35 101 51 0.0 6 51 102 27 0.0 6 26 1 103 42 0.0 6 42 104 41 0.0 6 40 1 105 37 0.0 6 37 106 18 0.0 6 18 107 29 0.0 6 29 108 46 0.0 6 46 109 27 0.0 6 26 1 110 38 0.0 6 38 111 26 0.0 6 26 112 44 0.0 6 44 113 44 0.0 6 44 114 26 0.0 6 26 115 31 0.0 6 31 116 47 0.0 6 47 117 23 0.0 6 23 118 29 0.0 6 29 119 21 0.0 6 21 120 14 0.0 6 14 121 22 0.0 6 22 122 24 0.0 6 24 123 33 0.0 6 33 124 52 0.0 6 52 125 53 0.0 6 53 126 26 0.0 6 26 127 34 0.0 6 34 128 35 0.0 6 35 129 32 0.0 6 32 130 54 0.0 6 54 131 61 0.0 6 61 132 91 0.0 6 91 133 44 0.0 6 43 1 134 58 0.0 6 57 1 135 40 0.0 6 40 136 31 0.0 6 31 137 33 0.0 6 33 138 50 0.0 6 50 139 38 0.0 6 38 140 32 0.0 6 32 141 35 0.0 6 35 142 41 0.0 6 41 143 31 0.0 6 31 144 39 0.0 6 39 145 39 0.0 6 39 146 38 0.0 6 38 147 45 0.0 6 45 148 24 0.0 6 24 149 29 0.0 6 29 150 42 0.0 6 42 151 35 0.0 6 35 152 27 0.0 6 27 153 20 0.0 6 20 154 32 0.0 6 32 155 38 0.0 6 38 156 34 0.0 6 34 157 10 0.0 6 10 158 20 0.0 6 20 159 21 0.0 6 21 160 35 0.0 6 35 161 40 0.0 6 40 162 20 0.0 6 20 163 18 0.0 6 18 164 32 0.0 6 32 165 43 0.0 6 43 166 29 0.0 6 29 167 44 0.0 6 43 0 0 0 0 0 1 168 34 0.0 6 34 169 55 0.0 6 55 170 28 0.0 6 28 171 22 0.0 6 22 172 33 0.0 6 33 173 21 0.0 6 21 174 13 0.0 6 13 175 23 0.0 6 23 176 34 0.0 6 34 177 24 0.0 6 24 178 14 0.0 6 14 179 7 0.0 6 7 180 5 0.0 6 5 181 9 0.0 6 9 182 16 0.0 6 16 183 33 0.0 6 33 184 21 0.0 6 21 185 8 0.0 6 8 186 26 0.0 6 26 187 19 0.0 6 19 188 21 0.0 6 21 189 21 0.0 6 21 190 10 0.0 6 10 191 15 0.0 6 15 192 12 0.0 6 12 193 11 0.0 6 11 194 18 0.0 6 18 195 14 0.0 6 14 196 17 0.0 6 17 197 10 0.0 6 10 198 22 0.0 6 22 199 18 0.0 6 18 200 13 0.0 6 13 201 10 0.0 6 10 202 17 0.0 6 17 203 14 0.0 6 14 204 9 0.0 6 8 0 0 0 0 0 1 205 6 0.0 6 6 206 10 0.0 6 10 207 10 0.0 6 10 208 20 0.0 6 20 209 14 0.0 6 14 210 20 0.0 6 20 211 9 0.0 6 9 212 13 0.0 6 13 213 18 0.0 6 18 214 10 0.0 6 10 215 9 0.0 6 9 216 15 0.0 6 15 217 8 0.0 6 8 218 8 0.0 6 8 219 4 0.0 6 4 220 5 0.0 6 5 221 9 0.0 6 9 222 10 0.0 6 10 223 11 0.0 6 11 224 6 0.0 6 6 225 8 0.0 6 8 226 14 0.0 6 14 227 7 0.0 6 7 228 8 0.0 6 8 229 6 0.0 6 6 230 4 0.0 6 4 231 5 0.0 6 5 232 4 0.0 6 4 233 9 0.0 6 9 234 8 0.0 6 8 235 9 0.0 6 9 236 7 0.0 6 7 237 2 0.0 6 2 238 8 0.0 6 8 239 2 0.0 6 2 240 4 0.0 6 4 241 1 0.0 6 1 242 5 0.0 6 5 243 1 0.0 6 1 244 2 0.0 6 2 245 2 0.0 6 2 246 3 0.0 6 3 247 2 0.0 6 2 248 5 0.0 6 5 249 2 0.0 6 2 250 3 0.0 6 3 251 4 0.0 6 4 252 2 0.0 6 2 253 3 0.0 6 3 254 4 0.0 6 4 255 3 0.0 6 3 256 2 0.0 6 2 257 1 0.0 6 1 258 1 0.0 6 1 259 2 0.0 6 2 260 3 0.0 6 3 261 1 0.0 6 1 262 2 0.0 6 2 263 2 0.0 6 2 264 1 0.0 6 1 266 2 0.0 6 2 267 3 0.0 6 3 268 1 0.0 6 1 269 1 0.0 6 1 270 2 0.0 6 2 271 2 0.0 6 2 272 1 0.0 6 1 273 1 0.0 6 1 274 5 0.0 6 5 275 2 0.0 6 2 276 1 0.0 6 1 277 1 0.0 6 1 279 1 0.0 6 1 281 2 0.0 6 2 282 1 0.0 6 1 283 2 0.0 6 2 286 1 0.0 6 1 287 2 0.0 6 2 288 1 0.0 6 1 293 1 0.0 6 1 294 2 0.0 6 2 296 1 0.0 6 1 297 1 0.0 6 1 299 1 0.0 6 1 303 1 0.0 6 1 309 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 147601 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 599.7 1 1 6 2 37.5 1 1 1 7 1 9.4 2 0 1 8 1 2.3 2 1 9 1 0.6 2 1 10 8 0.1 3 6 2 11 9 0.0 3 6 2 1 12 4 0.0 3 3 1 13 14 0.0 3 12 2 14 18 0.0 4 15 3 15 6 0.0 4 4 2 16 22 0.0 4 17 5 17 49 0.0 5 40 7 2 18 41 0.0 5 37 4 19 31 0.0 5 28 3 20 263 0.0 6 253 10 21 141 0.0 6 132 9 22 81 0.0 6 78 3 23 69 0.0 6 65 3 1 24 50 0.0 6 49 1 25 50 0.0 6 48 2 26 47 0.0 6 46 1 27 29 0.0 6 28 1 28 41 0.0 6 39 1 1 29 30 0.0 6 26 4 30 21 0.0 6 19 2 31 26 0.0 6 26 32 28 0.0 6 25 3 33 19 0.0 6 17 2 34 28 0.0 6 24 3 1 35 21 0.0 6 17 4 36 24 0.0 6 21 3 37 22 0.0 6 22 38 26 0.0 6 20 6 39 21 0.0 6 19 1 0 1 40 25 0.0 6 20 4 1 41 36 0.0 6 34 2 42 31 0.0 6 28 2 1 43 42 0.0 6 36 6 44 34 0.0 6 34 45 34 0.0 6 29 5 46 24 0.0 6 20 2 2 47 31 0.0 6 25 3 2 0 0 0 1 48 33 0.0 6 29 3 0 0 0 0 1 49 36 0.0 6 32 3 0 0 0 0 1 50 40 0.0 6 37 3 51 30 0.0 6 27 2 1 52 39 0.0 6 34 5 53 35 0.0 6 31 3 1 54 37 0.0 6 35 2 55 33 0.0 6 30 2 1 56 34 0.0 6 30 3 1 57 42 0.0 6 38 4 58 69 0.0 6 64 3 0 0 1 1 59 38 0.0 6 38 60 36 0.0 6 31 5 61 33 0.0 6 30 2 1 62 32 0.0 6 29 2 0 0 0 0 1 63 64 0.0 6 60 3 1 64 80 0.0 6 75 5 65 77 0.0 6 75 2 66 77 0.0 6 71 3 2 0 1 67 294 0.0 6 285 6 1 1 1 68 89 0.0 6 83 3 1 0 0 1 1 69 162 0.0 6 158 3 0 1 70 61 0.0 6 56 4 0 0 0 0 1 71 92 0.0 6 86 4 2 72 54 0.0 6 53 0 1 73 47 0.0 6 44 3 74 68 0.0 6 65 2 0 0 0 1 75 74 0.0 6 72 2 76 63 0.0 6 58 4 0 0 0 0 1 77 69 0.0 6 62 6 1 78 79 0.0 6 77 1 0 0 0 0 1 79 84 0.0 6 79 5 80 108 0.0 6 104 4 81 89 0.0 6 85 3 1 82 87 0.0 6 86 1 83 120 0.0 6 115 5 84 58 0.0 6 57 1 85 73 0.0 6 71 1 1 86 72 0.0 6 67 5 87 77 0.0 6 76 1 88 75 0.0 6 72 3 89 77 0.0 6 74 3 90 112 0.0 6 110 2 91 115 0.0 6 111 4 92 124 0.0 6 120 4 93 127 0.0 6 122 4 1 94 103 0.0 6 99 4 95 126 0.0 6 122 3 0 1 96 155 0.0 6 149 4 2 97 117 0.0 6 117 98 127 0.0 6 122 4 0 0 0 1 99 113 0.0 6 104 8 1 100 124 0.0 6 121 2 0 0 1 101 144 0.0 6 139 4 1 102 134 0.0 6 130 3 0 0 0 0 1 103 181 0.0 6 177 4 104 267 0.0 6 258 9 105 174 0.0 6 170 3 0 0 1 106 154 0.0 6 143 10 1 107 199 0.0 6 193 5 0 0 0 1 108 180 0.0 6 172 8 109 178 0.0 6 174 4 110 198 0.0 6 190 5 2 1 111 209 0.0 6 203 4 1 0 1 112 273 0.0 6 265 5 1 0 1 1 113 222 0.0 6 216 4 1 1 114 253 0.0 6 243 9 0 1 115 250 0.0 6 245 5 116 231 0.0 6 225 5 1 117 277 0.0 6 270 5 1 0 0 0 1 118 305 0.0 6 297 7 1 119 303 0.0 6 299 4 120 288 0.0 6 279 6 2 0 1 121 256 0.0 6 250 5 0 1 122 322 0.0 6 318 3 0 0 1 123 370 0.0 6 364 5 0 0 0 1 124 404 0.0 6 394 8 0 2 125 485 0.0 6 478 6 0 0 0 1 126 446 0.0 6 440 5 1 127 527 0.0 6 518 8 1 128 525 0.0 6 518 5 0 1 0 0 1 129 623 0.0 6 614 5 1 1 1 1 130 627 0.0 6 609 14 1 0 0 2 1 131 680 0.0 6 672 7 1 132 677 0.0 6 663 9 2 1 1 0 1 133 744 0.0 6 723 19 0 0 1 0 1 134 858 0.0 6 848 7 0 1 0 0 2 135 795 0.0 6 790 5 136 830 0.0 6 823 5 0 1 0 0 1 137 893 0.0 6 884 8 0 0 1 138 1009 0.0 6 1000 8 0 0 0 1 139 1255 0.0 6 1242 12 0 0 1 140 1527 0.0 6 1512 14 0 0 0 0 1 141 1815 0.0 6 1799 15 0 0 0 1 142 2130 0.0 6 2118 11 0 1 143 2360 0.0 6 2342 17 1 144 2527 0.0 6 2507 19 0 0 0 1 145 2559 0.0 6 2545 13 0 0 1 146 2833 0.0 6 2812 19 0 0 0 2 147 2816 0.0 6 2797 17 0 0 0 1 1 148 3429 0.0 6 3404 21 0 1 1 2 149 2963 0.0 6 2936 24 1 1 1 150 3299 0.0 6 3275 21 0 1 0 1 1 151 2824 0.0 6 2806 14 1 1 0 0 2 152 2791 0.0 6 2771 12 2 0 2 0 4 153 2768 0.0 6 2737 24 1 1 0 1 4 154 2444 0.0 6 2414 26 1 0 2 1 155 2660 0.0 6 2641 17 0 1 0 1 156 2341 0.0 6 2325 16 157 2715 0.0 6 2694 16 1 2 0 1 1 158 2658 0.0 6 2639 18 0 1 159 2478 0.0 6 2466 10 0 0 2 160 2615 0.0 6 2591 17 1 1 2 2 1 161 2267 0.0 6 2259 7 1 162 2126 0.0 6 2110 12 1 1 1 1 163 2270 0.0 6 2252 14 0 1 0 1 2 164 2208 0.0 6 2193 13 0 0 2 165 2008 0.0 6 1986 21 0 1 166 2499 0.0 6 2483 13 1 2 167 2215 0.0 6 2200 13 2 168 2706 0.0 6 2689 11 1 1 2 1 1 169 2248 0.0 6 2236 9 1 0 0 2 170 2132 0.0 6 2124 6 1 0 0 0 1 171 2196 0.0 6 2180 14 0 0 2 172 2329 0.0 6 2314 10 0 1 1 1 2 173 2249 0.0 6 2235 11 1 1 0 1 174 2049 0.0 6 2034 11 1 2 0 0 1 175 2017 0.0 6 2003 9 1 1 2 0 1 176 2353 0.0 6 2336 14 0 0 0 1 2 177 1703 0.0 6 1688 13 0 0 0 1 1 178 1895 0.0 6 1885 9 0 0 0 0 1 179 1579 0.0 6 1571 6 0 0 0 0 2 180 1425 0.0 6 1421 3 1 181 1505 0.0 6 1494 7 1 2 0 1 182 1307 0.0 6 1298 7 0 1 0 1 183 1968 0.0 6 1956 10 1 0 1 184 2394 0.0 6 2376 18 185 1891 0.0 6 1880 8 1 0 0 1 1 186 1321 0.0 6 1314 4 1 0 1 0 1 187 1260 0.0 6 1254 6 188 1182 0.0 6 1177 4 0 0 0 1 189 1158 0.0 6 1157 1 190 1105 0.0 6 1099 5 0 1 191 1007 0.0 6 994 7 0 2 1 1 2 192 885 0.0 6 880 4 0 0 0 0 1 193 851 0.0 6 849 1 0 0 0 0 1 194 675 0.0 6 672 3 195 594 0.0 6 591 3 196 900 0.0 6 894 5 0 1 197 640 0.0 6 635 3 1 0 0 0 1 198 676 0.0 6 674 2 199 565 0.0 6 561 4 200 481 0.0 6 479 2 201 389 0.0 6 387 1 1 202 461 0.0 6 460 1 203 400 0.0 6 399 1 204 335 0.0 6 333 1 0 0 1 205 304 0.0 6 302 2 206 313 0.0 6 312 1 207 312 0.0 6 309 3 208 236 0.0 6 235 0 0 0 0 1 209 243 0.0 6 242 1 210 207 0.0 6 207 211 201 0.0 6 199 2 212 244 0.0 6 243 1 213 232 0.0 6 228 2 0 0 1 0 1 214 261 0.0 6 261 215 172 0.0 6 171 1 216 138 0.0 6 137 1 217 161 0.0 6 159 2 218 108 0.0 6 107 1 219 98 0.0 6 98 220 80 0.0 6 80 221 97 0.0 6 96 1 222 78 0.0 6 76 2 223 69 0.0 6 69 224 41 0.0 6 38 1 0 0 1 0 1 225 50 0.0 6 49 1 226 35 0.0 6 34 1 227 34 0.0 6 34 228 29 0.0 6 29 229 30 0.0 6 29 0 1 230 20 0.0 6 20 231 21 0.0 6 21 232 19 0.0 6 19 233 14 0.0 6 14 234 18 0.0 6 18 235 8 0.0 6 8 236 7 0.0 6 7 237 6 0.0 6 6 238 11 0.0 6 11 239 8 0.0 6 8 240 5 0.0 6 5 241 13 0.0 6 13 242 9 0.0 6 9 243 3 0.0 6 3 244 1 0.0 6 1 245 2 0.0 6 2 246 1 0.0 6 1 248 3 0.0 6 3 249 2 0.0 6 2 250 1 0.0 6 1 251 1 0.0 6 1 252 1 0.0 6 1 253 1 0.0 6 1 277 4 0.0 6 4 278 7 0.0 6 7 279 138 0.0 6 136 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 5834 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 49 2 0.0 6 0 2 53 2 0.0 6 2 55 1 0.0 6 1 58 1 0.0 6 0 1 59 22 0.0 6 22 69 1 0.0 6 0 1 71 25 0.0 6 25 72 17 0.0 6 17 73 21 0.0 6 21 74 26 0.0 6 25 1 75 39 0.0 6 39 76 60 0.0 6 60 77 73 0.0 6 73 78 73 0.0 6 73 79 72 0.0 6 72 80 70 0.0 6 68 1 0 0 0 0 1 81 87 0.0 6 87 82 143 0.0 6 143 83 119 0.0 6 114 5 84 452 0.0 6 443 9 85 178 0.0 6 177 1 86 35 0.0 6 33 0 1 0 1 87 19 0.0 6 19 88 63 0.0 6 62 0 0 0 1 89 65 0.0 6 64 1 90 45 0.0 6 45 91 131 0.0 6 131 92 51 0.0 6 51 93 52 0.0 6 52 94 52 0.0 6 51 1 95 38 0.0 6 37 0 0 0 1 96 46 0.0 6 45 1 97 47 0.0 6 47 98 55 0.0 6 55 99 51 0.0 6 50 1 100 50 0.0 6 50 101 52 0.0 6 51 0 0 0 1 102 73 0.0 6 73 103 58 0.0 6 58 104 44 0.0 6 44 105 44 0.0 6 44 106 197 0.0 6 197 107 39 0.0 6 39 108 19 0.0 6 19 109 33 0.0 6 33 110 66 0.0 6 65 0 0 0 1 111 22 0.0 6 22 112 40 0.0 6 40 113 43 0.0 6 43 114 38 0.0 6 36 0 0 0 2 115 30 0.0 6 30 116 46 0.0 6 46 117 38 0.0 6 38 118 58 0.0 6 58 119 32 0.0 6 31 0 0 0 1 120 20 0.0 6 18 1 0 0 1 121 21 0.0 6 21 122 10 0.0 6 10 123 27 0.0 6 27 124 28 0.0 6 27 0 0 0 1 125 30 0.0 6 30 126 55 0.0 6 54 1 127 61 0.0 6 61 128 30 0.0 6 30 129 46 0.0 6 46 130 37 0.0 6 37 131 43 0.0 6 43 132 60 0.0 6 60 133 63 0.0 6 60 2 0 0 1 134 101 0.0 6 101 135 52 0.0 6 52 136 71 0.0 6 71 137 36 0.0 6 36 138 24 0.0 6 24 139 39 0.0 6 39 140 49 0.0 6 49 141 39 0.0 6 39 142 31 0.0 6 31 143 37 0.0 6 37 144 54 0.0 6 54 145 36 0.0 6 35 1 146 45 0.0 6 45 147 37 0.0 6 37 148 41 0.0 6 40 1 149 44 0.0 6 42 1 0 0 1 150 20 0.0 6 20 151 34 0.0 6 34 152 43 0.0 6 43 153 42 0.0 6 42 154 30 0.0 6 30 155 23 0.0 6 23 156 34 0.0 6 34 157 37 0.0 6 36 0 0 0 1 158 42 0.0 6 42 159 18 0.0 6 18 160 15 0.0 6 14 0 0 0 1 161 41 0.0 6 41 162 29 0.0 6 29 163 47 0.0 6 47 164 27 0.0 6 27 165 20 0.0 6 20 166 26 0.0 6 25 0 0 0 1 167 34 0.0 6 34 168 31 0.0 6 31 169 50 0.0 6 50 170 39 0.0 6 39 171 51 0.0 6 50 0 0 0 1 172 26 0.0 6 25 1 173 25 0.0 6 25 174 29 0.0 6 29 175 17 0.0 6 17 176 20 0.0 6 20 177 35 0.0 6 34 1 178 30 0.0 6 30 179 19 0.0 6 19 180 32 0.0 6 32 181 13 0.0 6 13 182 5 0.0 6 5 183 17 0.0 6 17 184 9 0.0 6 9 185 12 0.0 6 12 186 21 0.0 6 21 187 11 0.0 6 11 188 13 0.0 6 13 189 13 0.0 6 13 190 15 0.0 6 15 191 13 0.0 6 13 192 11 0.0 6 11 193 6 0.0 6 6 194 7 0.0 6 7 195 10 0.0 6 10 196 11 0.0 6 11 197 14 0.0 6 14 198 12 0.0 6 12 200 17 0.0 6 17 201 11 0.0 6 11 202 5 0.0 6 5 203 6 0.0 6 6 204 4 0.0 6 4 205 7 0.0 6 7 206 7 0.0 6 7 207 2 0.0 6 2 208 5 0.0 6 5 209 8 0.0 6 8 210 5 0.0 6 5 211 14 0.0 6 14 212 2 0.0 6 2 213 2 0.0 6 2 214 3 0.0 6 3 215 4 0.0 6 4 216 5 0.0 6 5 217 2 0.0 6 2 218 6 0.0 6 6 219 1 0.0 6 1 222 3 0.0 6 3 224 3 0.0 6 3 225 1 0.0 6 1 226 1 0.0 6 1 227 1 0.0 6 1 228 1 0.0 6 1 229 1 0.0 6 1 236 1 0.0 6 1 237 2 0.0 6 2 238 1 0.0 6 1 239 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_16_67_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_16_294_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_16_67_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_16_294_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.73 s (29 µs/read; 2.08 M reads/minute). === Summary === Total read pairs processed: 60,026 Read 1 with adapter: 60,023 (100.0%) Read 2 with adapter: 59,999 (100.0%) == Read fate breakdown == Pairs that were too short: 6 (0.0%) Pairs discarded as untrimmed: 30 (0.0%) Pairs written (passing filters): 59,990 (99.9%) Total basepairs processed: 28,661,276 bp Read 1: 16,134,450 bp Read 2: 12,526,826 bp Total written (filtered): 3,669,775 bp (12.8%) Read 1: 1,841,183 bp Read 2: 1,828,592 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 59357 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 58.6 1 1 7 2 3.7 2 2 8 1 0.9 2 1 9 2 0.2 2 1 1 16 1 0.0 4 1 17 1 0.0 5 0 1 18 1 0.0 5 1 19 1 0.0 5 1 20 52 0.0 6 51 1 21 24 0.0 6 24 22 14 0.0 6 14 23 14 0.0 6 14 24 13 0.0 6 12 0 1 25 15 0.0 6 14 0 0 1 26 4 0.0 6 4 27 12 0.0 6 11 1 28 3 0.0 6 3 29 4 0.0 6 3 1 30 3 0.0 6 3 31 1 0.0 6 1 32 2 0.0 6 2 33 6 0.0 6 6 35 3 0.0 6 3 36 4 0.0 6 4 37 5 0.0 6 5 38 2 0.0 6 2 40 3 0.0 6 3 41 4 0.0 6 4 42 3 0.0 6 3 43 1 0.0 6 1 44 4 0.0 6 4 45 8 0.0 6 7 1 46 2 0.0 6 2 48 2 0.0 6 2 49 2 0.0 6 2 50 1 0.0 6 1 51 5 0.0 6 4 1 52 3 0.0 6 3 53 4 0.0 6 2 2 54 2 0.0 6 2 55 3 0.0 6 3 56 1 0.0 6 1 57 2 0.0 6 1 1 58 5 0.0 6 5 59 2 0.0 6 2 60 3 0.0 6 3 61 1 0.0 6 1 62 3 0.0 6 3 63 3 0.0 6 3 64 2 0.0 6 2 65 1 0.0 6 1 66 2 0.0 6 2 67 5 0.0 6 4 1 68 2 0.0 6 2 69 3 0.0 6 3 70 2 0.0 6 2 71 1 0.0 6 1 72 5 0.0 6 4 0 1 73 7 0.0 6 7 74 6 0.0 6 6 75 8 0.0 6 8 76 1 0.0 6 1 77 7 0.0 6 6 1 78 9 0.0 6 9 79 6 0.0 6 6 80 3 0.0 6 3 82 2 0.0 6 2 83 5 0.0 6 5 84 3 0.0 6 2 1 85 1 0.0 6 1 86 4 0.0 6 3 1 87 5 0.0 6 5 88 4 0.0 6 4 89 2 0.0 6 2 90 3 0.0 6 3 91 3 0.0 6 2 1 92 3 0.0 6 3 94 4 0.0 6 4 96 1 0.0 6 1 97 5 0.0 6 5 98 5 0.0 6 5 99 8 0.0 6 8 100 7 0.0 6 7 101 5 0.0 6 5 102 14 0.0 6 14 103 7 0.0 6 7 104 4 0.0 6 4 105 9 0.0 6 9 106 7 0.0 6 7 107 6 0.0 6 6 108 9 0.0 6 9 109 3 0.0 6 2 1 110 8 0.0 6 8 111 6 0.0 6 6 112 8 0.0 6 7 1 113 10 0.0 6 10 114 13 0.0 6 13 115 6 0.0 6 6 116 8 0.0 6 7 1 117 10 0.0 6 10 118 9 0.0 6 7 2 119 10 0.0 6 6 3 0 0 0 1 120 11 0.0 6 11 121 11 0.0 6 11 122 22 0.0 6 20 2 123 21 0.0 6 20 1 124 30 0.0 6 30 125 7 0.0 6 7 126 11 0.0 6 11 127 14 0.0 6 12 2 128 13 0.0 6 12 0 1 129 9 0.0 6 8 1 130 10 0.0 6 10 131 15 0.0 6 15 132 22 0.0 6 19 2 1 133 7 0.0 6 7 134 17 0.0 6 17 135 15 0.0 6 15 136 16 0.0 6 16 137 45 0.0 6 43 2 138 27 0.0 6 27 139 19 0.0 6 18 1 140 44 0.0 6 44 141 82 0.0 6 82 142 803 0.0 6 801 2 143 185 0.0 6 185 144 438 0.0 6 436 1 1 145 668 0.0 6 667 1 146 111 0.0 6 110 1 147 105 0.0 6 105 148 460 0.0 6 459 1 149 78 0.0 6 78 150 34 0.0 6 31 3 151 30 0.0 6 29 1 152 28 0.0 6 28 153 28 0.0 6 27 1 154 43 0.0 6 43 155 55 0.0 6 55 156 151 0.0 6 150 1 157 674 0.0 6 671 3 158 113 0.0 6 113 159 40 0.0 6 38 1 0 0 0 1 160 29 0.0 6 24 5 161 48 0.0 6 48 162 36 0.0 6 36 163 65 0.0 6 65 164 105 0.0 6 104 1 165 819 0.0 6 818 1 166 171 0.0 6 171 167 43 0.0 6 42 1 168 38 0.0 6 38 169 31 0.0 6 29 2 170 94 0.0 6 92 2 171 57 0.0 6 55 2 172 46 0.0 6 46 173 61 0.0 6 61 174 70 0.0 6 69 1 175 257 0.0 6 256 1 176 65 0.0 6 62 2 1 177 90 0.0 6 90 178 115 0.0 6 111 4 179 138 0.0 6 136 1 1 180 251 0.0 6 251 181 115 0.0 6 114 0 0 0 1 182 637 0.0 6 636 1 183 132 0.0 6 132 184 92 0.0 6 91 0 1 185 86 0.0 6 81 5 186 94 0.0 6 92 2 187 83 0.0 6 81 2 188 119 0.0 6 118 1 189 175 0.0 6 172 3 190 955 0.0 6 952 3 191 198 0.0 6 195 3 192 96 0.0 6 94 2 193 99 0.0 6 97 2 194 99 0.0 6 98 1 195 117 0.0 6 116 1 196 162 0.0 6 162 197 168 0.0 6 166 2 198 269 0.0 6 264 5 199 373 0.0 6 367 6 200 1003 0.0 6 994 9 201 289 0.0 6 285 4 202 394 0.0 6 391 3 203 1263 0.0 6 1257 6 204 251 0.0 6 244 7 205 255 0.0 6 253 2 206 232 0.0 6 228 4 207 359 0.0 6 353 6 208 888 0.0 6 877 11 209 360 0.0 6 357 3 210 452 0.0 6 444 8 211 511 0.0 6 510 1 212 1149 0.0 6 1149 213 236 0.0 6 232 4 214 207 0.0 6 202 5 215 205 0.0 6 202 3 216 169 0.0 6 165 4 217 376 0.0 6 362 14 218 634 0.0 6 627 7 219 126 0.0 6 121 5 220 129 0.0 6 123 5 0 0 1 221 100 0.0 6 93 7 222 146 0.0 6 143 3 223 91 0.0 6 89 2 224 89 0.0 6 86 2 1 225 130 0.0 6 123 7 226 225 0.0 6 215 9 1 227 270 0.0 6 262 8 228 200 0.0 6 189 9 1 0 1 229 169 0.0 6 159 9 0 0 1 230 223 0.0 6 219 4 231 166 0.0 6 160 5 1 232 226 0.0 6 218 8 233 213 0.0 6 210 3 234 208 0.0 6 200 8 235 180 0.0 6 178 2 236 137 0.0 6 133 4 237 130 0.0 6 129 1 238 180 0.0 6 175 4 1 239 178 0.0 6 176 2 240 229 0.0 6 222 7 241 272 0.0 6 265 7 242 294 0.0 6 281 12 0 1 243 248 0.0 6 233 14 1 244 210 0.0 6 201 9 245 347 0.0 6 337 10 246 144 0.0 6 140 4 247 177 0.0 6 170 7 248 262 0.0 6 254 8 249 340 0.0 6 331 9 250 306 0.0 6 300 6 251 433 0.0 6 427 6 252 165 0.0 6 162 3 253 154 0.0 6 147 7 254 275 0.0 6 265 10 255 161 0.0 6 157 4 256 172 0.0 6 166 6 257 164 0.0 6 158 6 258 168 0.0 6 160 8 259 293 0.0 6 285 8 260 355 0.0 6 346 9 261 160 0.0 6 151 9 262 270 0.0 6 257 12 1 263 377 0.0 6 367 10 264 259 0.0 6 251 8 265 279 0.0 6 274 5 266 174 0.0 6 168 6 267 308 0.0 6 297 10 1 268 265 0.0 6 252 13 269 272 0.0 6 263 8 1 270 129 0.0 6 109 18 2 271 412 0.0 6 364 45 3 272 630 0.0 6 608 22 273 327 0.0 6 311 16 274 399 0.0 6 387 10 1 0 0 1 275 481 0.0 6 455 23 3 276 791 0.0 6 765 25 1 277 908 0.0 6 878 29 1 278 544 0.0 6 524 19 0 0 1 279 945 0.0 6 917 27 0 0 0 0 1 280 1246 0.0 6 1151 88 5 2 281 20488 0.0 6 20043 438 6 1 282 12 0.0 6 9 1 2 283 2 0.0 6 2 284 2 0.0 6 1 0 0 1 285 1 0.0 6 0 1 286 1 0.0 6 1 287 1 0.0 6 1 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 666 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 11 0.0 6 11 71 27 0.0 6 26 1 72 7 0.0 6 7 73 22 0.0 6 22 74 16 0.0 6 16 75 5 0.0 6 5 76 4 0.0 6 4 77 16 0.0 6 16 81 1 0.0 6 1 83 1 0.0 6 1 84 3 0.0 6 3 85 12 0.0 6 12 86 19 0.0 6 19 87 3 0.0 6 3 90 1 0.0 6 1 92 2 0.0 6 2 93 17 0.0 6 17 94 17 0.0 6 17 95 3 0.0 6 3 102 1 0.0 6 1 103 3 0.0 6 3 104 7 0.0 6 7 107 1 0.0 6 1 108 3 0.0 6 3 109 9 0.0 6 9 110 12 0.0 6 12 111 23 0.0 6 23 112 1 0.0 6 0 1 113 2 0.0 6 2 118 10 0.0 6 10 119 41 0.0 6 41 120 3 0.0 6 3 128 10 0.0 6 10 129 24 0.0 6 24 130 3 0.0 6 3 131 25 0.0 6 25 132 57 0.0 6 57 133 6 0.0 6 6 134 2 0.0 6 2 136 7 0.0 6 7 137 21 0.0 6 21 138 3 0.0 6 2 1 139 2 0.0 6 2 140 34 0.0 6 34 141 58 0.0 6 58 142 3 0.0 6 3 168 1 0.0 6 1 175 2 0.0 6 2 176 6 0.0 6 6 177 13 0.0 6 13 179 26 0.0 6 26 180 48 0.0 6 48 181 2 0.0 6 2 214 1 0.0 6 1 224 1 0.0 6 1 225 3 0.0 6 3 228 1 0.0 6 1 229 4 0.0 6 4 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 59294 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 14.7 1 1 7 1 3.7 2 1 10 1 0.1 3 1 11 5 0.0 3 4 0 1 12 3 0.0 3 1 2 13 17 0.0 3 17 14 7 0.0 4 5 1 1 16 15 0.0 4 14 1 17 3 0.0 5 3 18 7 0.0 5 6 0 1 19 5 0.0 5 4 1 20 51 0.0 6 45 6 21 55 0.0 6 53 2 22 21 0.0 6 20 1 23 23 0.0 6 22 1 24 11 0.0 6 11 25 10 0.0 6 10 26 7 0.0 6 6 1 27 4 0.0 6 4 28 4 0.0 6 4 29 12 0.0 6 12 30 8 0.0 6 8 31 5 0.0 6 4 1 32 9 0.0 6 9 33 7 0.0 6 7 34 7 0.0 6 7 35 8 0.0 6 6 1 1 36 5 0.0 6 4 1 37 11 0.0 6 11 38 7 0.0 6 7 39 3 0.0 6 1 2 40 9 0.0 6 8 1 41 5 0.0 6 5 42 8 0.0 6 7 1 43 5 0.0 6 3 2 44 11 0.0 6 11 45 8 0.0 6 6 2 46 9 0.0 6 8 1 47 12 0.0 6 11 1 48 7 0.0 6 7 49 13 0.0 6 13 50 15 0.0 6 13 2 51 4 0.0 6 4 52 14 0.0 6 13 1 53 8 0.0 6 7 1 54 10 0.0 6 8 2 55 14 0.0 6 13 0 1 56 8 0.0 6 7 1 57 13 0.0 6 13 58 10 0.0 6 9 0 0 1 59 8 0.0 6 8 60 16 0.0 6 15 1 61 3 0.0 6 2 1 62 14 0.0 6 13 1 63 5 0.0 6 5 64 15 0.0 6 15 65 4 0.0 6 4 66 16 0.0 6 16 67 13 0.0 6 13 68 28 0.0 6 27 1 69 88 0.0 6 87 0 0 0 0 1 70 24 0.0 6 20 3 1 71 53 0.0 6 51 2 72 26 0.0 6 23 1 2 73 11 0.0 6 11 74 21 0.0 6 19 2 75 17 0.0 6 16 1 76 34 0.0 6 33 1 77 21 0.0 6 21 78 20 0.0 6 19 0 0 0 1 79 14 0.0 6 12 2 80 20 0.0 6 20 81 16 0.0 6 16 82 20 0.0 6 19 1 83 32 0.0 6 28 4 84 45 0.0 6 41 4 85 32 0.0 6 31 1 86 17 0.0 6 17 87 24 0.0 6 21 3 88 21 0.0 6 19 2 89 18 0.0 6 18 90 16 0.0 6 16 91 29 0.0 6 28 1 92 19 0.0 6 18 1 93 38 0.0 6 36 2 94 52 0.0 6 50 2 95 26 0.0 6 26 96 30 0.0 6 30 97 32 0.0 6 32 98 26 0.0 6 23 3 99 30 0.0 6 30 100 30 0.0 6 29 0 0 0 1 101 35 0.0 6 34 1 102 47 0.0 6 43 3 0 0 0 1 103 72 0.0 6 71 1 104 46 0.0 6 45 1 105 42 0.0 6 39 2 1 106 56 0.0 6 55 1 107 50 0.0 6 45 4 1 108 44 0.0 6 41 2 0 1 109 45 0.0 6 44 0 1 110 47 0.0 6 45 2 111 61 0.0 6 59 1 1 112 40 0.0 6 38 2 113 35 0.0 6 35 114 78 0.0 6 76 2 115 39 0.0 6 35 4 116 61 0.0 6 59 1 1 117 38 0.0 6 38 118 55 0.0 6 54 1 119 85 0.0 6 82 3 120 93 0.0 6 88 5 121 52 0.0 6 50 2 122 81 0.0 6 76 5 123 83 0.0 6 79 4 124 92 0.0 6 91 1 125 72 0.0 6 68 2 1 1 126 76 0.0 6 73 2 1 127 65 0.0 6 64 0 1 128 83 0.0 6 81 2 129 64 0.0 6 61 3 130 86 0.0 6 85 1 131 128 0.0 6 124 2 1 0 1 132 147 0.0 6 143 4 133 124 0.0 6 122 2 134 197 0.0 6 194 3 135 293 0.0 6 286 7 136 215 0.0 6 207 8 137 312 0.0 6 307 5 138 272 0.0 6 268 4 139 766 0.0 6 764 2 140 271 0.0 6 266 4 1 141 540 0.0 6 537 2 1 142 748 0.0 6 740 7 0 0 0 1 143 304 0.0 6 296 8 144 404 0.0 6 388 15 0 0 1 145 506 0.0 6 501 5 146 233 0.0 6 227 5 1 147 149 0.0 6 145 4 148 206 0.0 6 203 3 149 297 0.0 6 292 4 0 1 150 499 0.0 6 488 10 0 0 1 151 265 0.0 6 261 4 152 648 0.0 6 638 7 0 2 1 153 488 0.0 6 478 10 154 820 0.0 6 807 12 1 155 521 0.0 6 517 4 156 364 0.0 6 356 8 157 502 0.0 6 491 10 1 158 640 0.0 6 632 8 159 363 0.0 6 358 3 0 0 0 1 1 160 541 0.0 6 531 10 161 380 0.0 6 374 6 162 678 0.0 6 668 10 163 374 0.0 6 366 8 164 315 0.0 6 308 7 165 422 0.0 6 409 12 1 166 501 0.0 6 486 13 2 167 319 0.0 6 311 7 0 0 1 168 649 0.0 6 629 17 1 0 2 169 292 0.0 6 290 1 1 170 496 0.0 6 484 12 171 692 0.0 6 675 16 0 0 1 172 320 0.0 6 308 10 1 0 0 1 173 427 0.0 6 418 8 0 1 174 609 0.0 6 599 9 1 175 899 0.0 6 866 31 1 0 1 176 686 0.0 6 670 15 1 177 775 0.0 6 759 16 178 714 0.0 6 695 17 2 179 786 0.0 6 773 12 0 0 1 180 709 0.0 6 686 21 0 0 0 1 1 181 702 0.0 6 687 13 1 1 182 366 0.0 6 355 11 183 706 0.0 6 689 17 184 278 0.0 6 273 5 185 430 0.0 6 421 8 1 186 1499 0.0 6 1459 39 1 187 1350 0.0 6 1319 30 1 188 1070 0.0 6 1045 24 1 189 924 0.0 6 897 26 0 0 0 1 190 1449 0.0 6 1423 24 1 1 191 1148 0.0 6 1116 31 0 0 1 192 936 0.0 6 918 16 1 0 1 193 671 0.0 6 650 18 3 194 952 0.0 6 925 25 2 195 1146 0.0 6 1112 32 1 0 1 196 2066 0.0 6 2016 48 1 0 1 197 540 0.0 6 524 12 3 0 1 198 1755 0.0 6 1695 56 1 0 1 2 199 2436 0.0 6 2361 71 1 0 3 200 778 0.0 6 749 27 1 0 1 201 820 0.0 6 793 23 1 1 2 202 519 0.0 6 500 16 1 1 1 203 537 0.0 6 517 19 1 204 744 0.0 6 724 19 0 0 1 205 269 0.0 6 261 8 206 271 0.0 6 263 8 207 142 0.0 6 137 5 208 302 0.0 6 292 9 1 209 283 0.0 6 271 11 0 1 210 240 0.0 6 229 11 211 370 0.0 6 357 13 212 352 0.0 6 345 7 213 107 0.0 6 104 2 1 214 305 0.0 6 297 7 0 0 1 215 281 0.0 6 271 10 216 620 0.0 6 609 10 0 0 1 217 458 0.0 6 443 14 1 218 302 0.0 6 294 8 219 306 0.0 6 299 7 220 213 0.0 6 205 6 2 221 330 0.0 6 322 8 222 334 0.0 6 314 20 223 777 0.0 6 759 18 224 163 0.0 6 157 6 225 323 0.0 6 316 7 226 257 0.0 6 248 8 1 227 170 0.0 6 161 9 228 106 0.0 6 100 6 229 199 0.0 6 192 7 230 118 0.0 6 114 4 231 162 0.0 6 156 5 1 232 203 0.0 6 198 5 233 278 0.0 6 272 6 234 122 0.0 6 121 1 235 56 0.0 6 56 236 46 0.0 6 43 3 237 41 0.0 6 41 238 8 0.0 6 7 1 239 17 0.0 6 16 1 240 103 0.0 6 96 7 241 52 0.0 6 51 1 242 101 0.0 6 94 6 1 243 148 0.0 6 142 6 244 63 0.0 6 61 2 245 33 0.0 6 32 1 246 27 0.0 6 25 2 247 10 0.0 6 8 2 248 18 0.0 6 17 1 249 21 0.0 6 20 1 250 20 0.0 6 19 1 251 19 0.0 6 18 1 252 2 0.0 6 2 253 13 0.0 6 13 254 5 0.0 6 5 255 2 0.0 6 2 256 1 0.0 6 1 257 1 0.0 6 1 278 2 0.0 6 2 279 6 0.0 6 6 280 19 0.0 6 18 1 281 106 0.0 6 103 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 705 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 47 1 0.0 6 0 1 61 1 0.0 6 0 1 69 2 0.0 6 2 70 12 0.0 6 12 71 36 0.0 6 36 72 3 0.0 6 3 73 21 0.0 6 21 74 23 0.0 6 23 75 2 0.0 6 1 0 0 0 1 76 2 0.0 6 2 77 15 0.0 6 15 78 2 0.0 6 2 84 1 0.0 6 1 85 9 0.0 6 9 86 25 0.0 6 25 87 1 0.0 6 1 89 1 0.0 6 1 92 2 0.0 6 2 93 2 0.0 6 2 94 32 0.0 6 32 98 1 0.0 6 0 0 0 0 1 104 6 0.0 6 6 105 1 0.0 6 1 108 2 0.0 6 2 109 9 0.0 6 9 110 8 0.0 6 8 111 40 0.0 6 40 118 19 0.0 6 18 1 119 32 0.0 6 32 120 1 0.0 6 1 122 1 0.0 6 0 0 0 1 127 1 0.0 6 1 128 4 0.0 6 4 129 30 0.0 6 30 130 4 0.0 6 4 131 23 0.0 6 23 132 65 0.0 6 65 133 6 0.0 6 6 134 1 0.0 6 1 135 2 0.0 6 2 136 7 0.0 6 7 137 31 0.0 6 31 139 2 0.0 6 2 140 19 0.0 6 19 141 77 0.0 6 77 142 2 0.0 6 2 175 3 0.0 6 3 176 5 0.0 6 5 177 13 0.0 6 13 178 2 0.0 6 2 179 18 0.0 6 18 180 72 0.0 6 72 181 5 0.0 6 4 0 0 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_17_68_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_17_295_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_17_68_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_17_295_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.31 s (27 µs/read; 2.25 M reads/minute). === Summary === Total read pairs processed: 86,873 Read 1 with adapter: 86,865 (100.0%) Read 2 with adapter: 86,815 (99.9%) == Read fate breakdown == Pairs that were too short: 12 (0.0%) Pairs discarded as untrimmed: 65 (0.1%) Pairs written (passing filters): 86,796 (99.9%) Total basepairs processed: 42,288,700 bp Read 1: 24,237,467 bp Read 2: 18,051,233 bp Total written (filtered): 5,176,577 bp (12.2%) Read 1: 2,636,269 bp Read 2: 2,540,308 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 86311 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1357.4 0 1 1 4 1 339.3 1 0 1 5 2 84.8 1 2 6 3 21.2 1 0 0 3 7 2 5.3 2 1 0 1 8 1 1.3 2 1 9 2 0.3 2 2 10 1 0.1 3 1 15 3 0.0 4 2 1 16 4 0.0 4 3 1 17 1 0.0 5 1 19 3 0.0 5 1 2 20 66 0.0 6 62 4 21 37 0.0 6 35 2 22 28 0.0 6 28 23 27 0.0 6 27 24 13 0.0 6 13 25 15 0.0 6 14 1 26 9 0.0 6 9 27 7 0.0 6 7 28 7 0.0 6 6 0 0 0 1 29 5 0.0 6 4 1 30 6 0.0 6 5 0 1 31 3 0.0 6 3 32 4 0.0 6 4 33 3 0.0 6 3 34 3 0.0 6 3 35 4 0.0 6 3 1 36 6 0.0 6 6 37 4 0.0 6 4 38 7 0.0 6 5 1 0 0 0 1 39 8 0.0 6 8 40 5 0.0 6 5 41 4 0.0 6 4 42 2 0.0 6 2 43 4 0.0 6 3 1 44 5 0.0 6 4 0 1 45 6 0.0 6 6 46 6 0.0 6 5 1 47 4 0.0 6 4 48 4 0.0 6 4 49 8 0.0 6 8 50 3 0.0 6 3 51 3 0.0 6 3 52 3 0.0 6 2 1 53 5 0.0 6 4 1 54 5 0.0 6 5 55 2 0.0 6 2 56 7 0.0 6 7 57 9 0.0 6 8 0 0 0 1 58 5 0.0 6 5 60 6 0.0 6 4 2 61 2 0.0 6 2 62 3 0.0 6 3 63 7 0.0 6 6 0 1 64 4 0.0 6 4 65 2 0.0 6 2 66 1 0.0 6 1 67 1 0.0 6 1 68 1 0.0 6 1 69 7 0.0 6 7 70 2 0.0 6 2 71 5 0.0 6 5 72 6 0.0 6 6 73 3 0.0 6 3 74 2 0.0 6 2 75 4 0.0 6 4 76 10 0.0 6 10 77 6 0.0 6 5 1 78 8 0.0 6 5 3 79 8 0.0 6 7 1 80 7 0.0 6 6 1 81 5 0.0 6 5 83 2 0.0 6 1 1 84 3 0.0 6 3 85 6 0.0 6 6 86 14 0.0 6 14 87 7 0.0 6 7 88 6 0.0 6 6 89 7 0.0 6 7 90 3 0.0 6 3 91 8 0.0 6 8 92 10 0.0 6 9 1 93 5 0.0 6 5 94 4 0.0 6 4 95 2 0.0 6 2 96 4 0.0 6 4 97 9 0.0 6 9 98 10 0.0 6 10 99 6 0.0 6 6 100 6 0.0 6 5 1 101 9 0.0 6 9 102 13 0.0 6 10 3 103 15 0.0 6 15 104 10 0.0 6 9 1 105 9 0.0 6 9 106 8 0.0 6 7 1 107 13 0.0 6 12 1 108 18 0.0 6 17 1 109 10 0.0 6 10 110 6 0.0 6 6 111 13 0.0 6 11 2 112 10 0.0 6 10 113 9 0.0 6 8 1 114 19 0.0 6 17 1 0 0 0 0 1 115 18 0.0 6 17 1 116 15 0.0 6 15 117 13 0.0 6 12 0 0 1 118 7 0.0 6 6 1 119 8 0.0 6 8 120 13 0.0 6 13 121 8 0.0 6 7 0 1 122 25 0.0 6 24 1 123 26 0.0 6 25 1 124 15 0.0 6 13 2 125 11 0.0 6 11 126 27 0.0 6 27 127 20 0.0 6 19 1 128 35 0.0 6 35 129 21 0.0 6 19 2 130 26 0.0 6 26 131 25 0.0 6 24 1 132 34 0.0 6 32 2 133 16 0.0 6 15 1 134 20 0.0 6 19 1 135 22 0.0 6 22 136 26 0.0 6 26 137 23 0.0 6 21 2 138 36 0.0 6 35 1 139 43 0.0 6 42 1 140 119 0.0 6 119 141 159 0.0 6 158 1 142 218 0.0 6 218 143 153 0.0 6 152 1 144 153 0.0 6 150 2 1 145 204 0.0 6 203 1 146 227 0.0 6 223 4 147 349 0.0 6 346 2 0 0 0 1 148 317 0.0 6 315 1 1 149 135 0.0 6 134 0 1 150 308 0.0 6 307 1 151 217 0.0 6 217 152 126 0.0 6 125 0 0 0 0 1 153 164 0.0 6 164 154 160 0.0 6 160 155 219 0.0 6 218 1 156 195 0.0 6 195 157 228 0.0 6 227 1 158 143 0.0 6 142 0 1 159 176 0.0 6 175 1 160 266 0.0 6 264 1 1 161 238 0.0 6 237 1 162 152 0.0 6 152 163 242 0.0 6 239 3 164 199 0.0 6 197 1 1 165 115 0.0 6 115 166 181 0.0 6 179 2 167 210 0.0 6 210 168 112 0.0 6 112 169 186 0.0 6 185 1 170 215 0.0 6 213 2 171 291 0.0 6 290 1 172 282 0.0 6 280 2 173 176 0.0 6 176 174 242 0.0 6 241 1 175 227 0.0 6 226 1 176 140 0.0 6 138 2 177 192 0.0 6 190 1 0 1 178 493 0.0 6 491 2 179 246 0.0 6 245 1 180 165 0.0 6 162 3 181 192 0.0 6 191 1 182 232 0.0 6 229 3 183 151 0.0 6 149 2 184 214 0.0 6 210 4 185 351 0.0 6 350 1 186 205 0.0 6 202 3 187 213 0.0 6 212 1 188 247 0.0 6 245 2 189 254 0.0 6 251 3 190 184 0.0 6 182 2 191 228 0.0 6 225 3 192 260 0.0 6 257 3 193 224 0.0 6 221 3 194 272 0.0 6 271 1 195 282 0.0 6 281 1 196 578 0.0 6 578 197 378 0.0 6 374 4 198 362 0.0 6 360 2 199 556 0.0 6 549 6 1 200 390 0.0 6 389 1 201 393 0.0 6 390 2 1 202 583 0.0 6 573 10 203 359 0.0 6 355 4 204 303 0.0 6 299 4 205 392 0.0 6 387 5 206 260 0.0 6 256 4 207 414 0.0 6 408 6 208 444 0.0 6 437 6 0 1 209 434 0.0 6 428 6 210 301 0.0 6 293 7 1 211 266 0.0 6 260 6 212 287 0.0 6 286 1 213 252 0.0 6 250 2 214 269 0.0 6 264 5 215 242 0.0 6 239 3 216 268 0.0 6 266 2 217 451 0.0 6 445 6 218 285 0.0 6 283 2 219 270 0.0 6 264 6 220 303 0.0 6 300 2 1 221 295 0.0 6 294 1 222 291 0.0 6 286 5 223 225 0.0 6 221 3 0 0 0 1 224 227 0.0 6 226 1 225 207 0.0 6 198 9 226 337 0.0 6 334 3 227 332 0.0 6 326 5 0 1 228 310 0.0 6 305 5 229 294 0.0 6 291 3 230 254 0.0 6 248 6 231 222 0.0 6 215 6 1 232 206 0.0 6 204 2 233 216 0.0 6 213 2 0 0 0 1 234 198 0.0 6 197 1 235 188 0.0 6 186 2 236 131 0.0 6 129 2 237 166 0.0 6 163 3 238 138 0.0 6 135 2 1 239 192 0.0 6 183 9 240 324 0.0 6 312 11 1 241 474 0.0 6 464 9 0 1 242 299 0.0 6 285 14 243 349 0.0 6 338 11 244 283 0.0 6 272 11 245 423 0.0 6 412 11 246 118 0.0 6 116 2 247 202 0.0 6 196 6 248 139 0.0 6 132 7 249 289 0.0 6 277 12 250 224 0.0 6 215 8 0 0 0 1 251 205 0.0 6 198 7 252 204 0.0 6 201 3 253 175 0.0 6 166 9 254 257 0.0 6 251 6 255 167 0.0 6 161 6 256 172 0.0 6 166 6 257 145 0.0 6 140 4 0 1 258 184 0.0 6 167 17 259 232 0.0 6 219 12 1 260 292 0.0 6 281 11 261 157 0.0 6 147 9 1 262 260 0.0 6 243 16 1 263 281 0.0 6 277 4 264 149 0.0 6 146 3 265 191 0.0 6 186 5 266 97 0.0 6 90 7 267 170 0.0 6 163 7 268 147 0.0 6 135 12 269 191 0.0 6 183 7 1 270 72 0.0 6 64 6 2 271 293 0.0 6 269 23 1 272 578 0.0 6 568 10 273 247 0.0 6 235 11 1 274 407 0.0 6 397 10 275 157 0.0 6 145 11 1 276 687 0.0 6 655 31 1 277 1916 0.0 6 1864 47 4 1 278 732 0.0 6 715 16 0 0 0 1 279 1566 0.0 6 1527 39 280 901 0.0 6 736 150 14 1 281 45129 0.0 6 44290 826 8 1 4 282 19 0.0 6 11 2 6 283 3 0.0 6 2 0 0 0 0 1 284 1 0.0 6 1 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 554 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 11 0.0 6 11 67 1 0.0 6 0 0 0 0 0 0 1 69 2 0.0 6 2 70 5 0.0 6 5 71 7 0.0 6 7 72 5 0.0 6 5 73 6 0.0 6 6 74 2 0.0 6 2 75 6 0.0 6 6 76 7 0.0 6 7 77 2 0.0 6 2 78 8 0.0 6 8 79 6 0.0 6 6 80 8 0.0 6 8 81 6 0.0 6 6 82 9 0.0 6 9 83 2 0.0 6 2 84 4 0.0 6 4 85 6 0.0 6 6 86 5 0.0 6 5 87 6 0.0 6 6 88 4 0.0 6 4 89 6 0.0 6 6 90 8 0.0 6 8 91 3 0.0 6 3 92 5 0.0 6 5 93 9 0.0 6 9 94 2 0.0 6 2 95 1 0.0 6 1 96 4 0.0 6 4 97 7 0.0 6 7 98 2 0.0 6 2 99 2 0.0 6 2 100 9 0.0 6 9 101 7 0.0 6 7 102 10 0.0 6 9 1 103 3 0.0 6 3 104 3 0.0 6 3 105 5 0.0 6 5 106 2 0.0 6 2 107 6 0.0 6 6 108 9 0.0 6 9 109 7 0.0 6 7 110 3 0.0 6 3 111 7 0.0 6 7 112 5 0.0 6 5 113 2 0.0 6 2 114 3 0.0 6 3 115 7 0.0 6 7 116 3 0.0 6 3 117 6 0.0 6 6 118 2 0.0 6 2 119 2 0.0 6 2 120 2 0.0 6 2 122 4 0.0 6 4 123 3 0.0 6 3 124 5 0.0 6 5 125 6 0.0 6 6 126 14 0.0 6 14 127 5 0.0 6 5 128 7 0.0 6 7 129 6 0.0 6 6 130 1 0.0 6 1 131 6 0.0 6 6 132 15 0.0 6 15 133 4 0.0 6 4 134 4 0.0 6 4 135 3 0.0 6 3 136 3 0.0 6 3 137 5 0.0 6 5 138 2 0.0 6 2 139 7 0.0 6 7 140 4 0.0 6 4 141 5 0.0 6 5 142 7 0.0 6 7 143 5 0.0 6 5 144 4 0.0 6 4 145 2 0.0 6 2 146 7 0.0 6 7 147 1 0.0 6 1 148 3 0.0 6 3 149 4 0.0 6 4 150 3 0.0 6 3 152 4 0.0 6 4 153 6 0.0 6 6 154 5 0.0 6 5 155 4 0.0 6 4 156 2 0.0 6 2 157 3 0.0 6 3 158 4 0.0 6 4 159 2 0.0 6 2 160 6 0.0 6 6 161 8 0.0 6 8 162 1 0.0 6 1 163 3 0.0 6 3 164 2 0.0 6 2 165 1 0.0 6 1 167 2 0.0 6 2 168 2 0.0 6 2 169 2 0.0 6 2 170 4 0.0 6 4 172 1 0.0 6 1 173 1 0.0 6 1 174 2 0.0 6 2 175 4 0.0 6 4 177 2 0.0 6 2 178 1 0.0 6 1 179 4 0.0 6 4 180 2 0.0 6 2 182 2 0.0 6 2 183 3 0.0 6 3 184 2 0.0 6 2 185 1 0.0 6 1 186 5 0.0 6 5 187 4 0.0 6 4 188 1 0.0 6 1 189 1 0.0 6 1 190 4 0.0 6 4 191 1 0.0 6 1 192 2 0.0 6 2 194 2 0.0 6 2 195 3 0.0 6 3 197 1 0.0 6 1 198 1 0.0 6 1 200 1 0.0 6 1 201 1 0.0 6 1 203 1 0.0 6 1 206 3 0.0 6 3 212 2 0.0 6 2 213 2 0.0 6 2 215 1 0.0 6 1 217 1 0.0 6 1 218 1 0.0 6 1 219 2 0.0 6 2 221 1 0.0 6 1 227 1 0.0 6 1 230 1 0.0 6 1 231 1 0.0 6 1 232 1 0.0 6 1 235 1 0.0 6 1 236 1 0.0 6 1 251 1 0.0 6 1 255 1 0.0 6 1 261 1 0.0 6 1 263 1 0.0 6 1 272 1 0.0 6 1 289 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 86250 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1357.4 0 1 4 1 339.3 1 0 1 6 1 21.2 1 1 8 1 1.3 2 0 1 10 1 0.1 3 1 11 2 0.0 3 2 12 3 0.0 3 3 13 4 0.0 3 3 1 14 14 0.0 4 13 0 0 1 15 2 0.0 4 1 0 1 16 13 0.0 4 10 3 17 28 0.0 5 26 2 18 8 0.0 5 7 1 19 60 0.0 5 57 2 1 20 119 0.0 6 111 7 1 21 82 0.0 6 81 1 22 55 0.0 6 53 2 23 41 0.0 6 36 4 1 24 24 0.0 6 23 0 0 0 0 1 25 23 0.0 6 23 26 15 0.0 6 12 2 1 27 15 0.0 6 14 1 28 16 0.0 6 14 1 1 29 24 0.0 6 21 3 30 24 0.0 6 22 2 31 12 0.0 6 12 32 17 0.0 6 16 1 33 26 0.0 6 23 2 1 34 14 0.0 6 14 35 10 0.0 6 10 36 15 0.0 6 15 37 3 0.0 6 3 38 25 0.0 6 23 1 0 0 0 0 1 39 15 0.0 6 14 1 40 14 0.0 6 14 41 10 0.0 6 10 42 8 0.0 6 8 43 15 0.0 6 15 44 22 0.0 6 19 3 45 24 0.0 6 21 3 46 24 0.0 6 23 1 47 16 0.0 6 12 3 0 0 1 48 26 0.0 6 21 5 49 43 0.0 6 39 3 0 0 1 50 25 0.0 6 24 1 51 23 0.0 6 19 3 1 52 26 0.0 6 22 4 53 18 0.0 6 16 2 54 20 0.0 6 19 1 55 40 0.0 6 35 4 0 0 1 56 28 0.0 6 24 3 0 0 0 1 57 46 0.0 6 40 6 58 20 0.0 6 14 4 0 0 0 2 59 16 0.0 6 15 1 60 33 0.0 6 30 3 61 17 0.0 6 15 2 62 32 0.0 6 32 63 20 0.0 6 20 64 19 0.0 6 19 65 9 0.0 6 8 1 66 33 0.0 6 31 0 1 0 1 67 31 0.0 6 26 4 1 68 24 0.0 6 22 2 69 50 0.0 6 45 2 2 0 1 70 173 0.0 6 166 7 71 45 0.0 6 41 3 1 72 100 0.0 6 96 2 0 0 1 0 1 73 32 0.0 6 29 3 74 39 0.0 6 38 1 75 25 0.0 6 21 2 0 1 1 76 28 0.0 6 28 77 34 0.0 6 33 0 0 0 0 0 1 78 29 0.0 6 27 2 79 15 0.0 6 15 80 46 0.0 6 43 3 81 31 0.0 6 29 2 82 52 0.0 6 49 3 83 42 0.0 6 41 1 84 62 0.0 6 59 3 85 54 0.0 6 48 6 86 64 0.0 6 63 1 87 58 0.0 6 56 1 1 88 25 0.0 6 20 4 0 0 1 89 40 0.0 6 36 3 0 1 90 45 0.0 6 43 2 91 54 0.0 6 50 4 92 29 0.0 6 28 1 93 45 0.0 6 43 2 94 103 0.0 6 97 3 1 0 1 1 95 41 0.0 6 37 3 1 96 59 0.0 6 54 4 1 97 51 0.0 6 48 3 98 50 0.0 6 48 2 99 81 0.0 6 79 2 100 77 0.0 6 73 3 0 0 0 0 1 101 71 0.0 6 68 3 102 78 0.0 6 69 7 0 0 2 103 98 0.0 6 89 7 2 104 110 0.0 6 101 7 1 0 1 105 93 0.0 6 87 5 1 106 72 0.0 6 67 5 107 108 0.0 6 106 2 108 90 0.0 6 85 4 0 0 1 109 60 0.0 6 56 2 0 1 0 0 1 110 75 0.0 6 71 3 1 111 87 0.0 6 79 7 1 112 91 0.0 6 90 1 113 77 0.0 6 76 1 114 86 0.0 6 84 2 115 105 0.0 6 103 1 1 116 90 0.0 6 83 7 117 97 0.0 6 93 3 0 1 118 93 0.0 6 91 0 1 0 1 119 113 0.0 6 108 3 2 120 138 0.0 6 135 3 121 103 0.0 6 99 2 0 0 1 1 122 98 0.0 6 91 7 123 116 0.0 6 112 3 0 1 124 129 0.0 6 119 10 125 113 0.0 6 110 3 126 74 0.0 6 72 2 127 110 0.0 6 107 3 128 161 0.0 6 154 5 0 0 1 1 129 129 0.0 6 125 2 2 130 121 0.0 6 118 2 1 131 169 0.0 6 163 3 0 0 3 132 186 0.0 6 179 5 2 133 173 0.0 6 168 4 0 1 134 180 0.0 6 176 4 135 372 0.0 6 362 6 3 0 0 0 1 136 244 0.0 6 235 8 1 137 301 0.0 6 290 11 138 301 0.0 6 294 7 139 372 0.0 6 366 6 140 434 0.0 6 424 9 1 141 416 0.0 6 411 5 142 564 0.0 6 547 13 1 2 1 143 537 0.0 6 521 15 0 0 1 144 704 0.0 6 682 20 1 1 145 588 0.0 6 577 9 1 0 1 146 549 0.0 6 539 9 1 147 427 0.0 6 422 3 1 0 0 1 148 508 0.0 6 501 6 0 0 0 0 1 149 647 0.0 6 634 10 2 0 0 1 150 714 0.0 6 695 14 1 2 2 151 582 0.0 6 572 10 152 874 0.0 6 854 18 1 0 1 153 1011 0.0 6 984 26 0 0 0 0 1 154 717 0.0 6 702 15 155 889 0.0 6 878 10 0 0 1 156 761 0.0 6 744 16 0 1 157 656 0.0 6 641 14 0 0 1 158 810 0.0 6 800 10 159 555 0.0 6 543 12 160 723 0.0 6 709 11 1 0 1 1 161 610 0.0 6 604 5 0 1 162 628 0.0 6 617 11 163 714 0.0 6 698 14 1 1 164 708 0.0 6 697 9 1 0 1 165 794 0.0 6 773 20 1 166 787 0.0 6 765 20 0 0 1 1 167 578 0.0 6 561 15 1 1 168 746 0.0 6 731 15 169 603 0.0 6 595 7 1 170 527 0.0 6 515 11 0 0 1 171 834 0.0 6 810 21 2 1 172 461 0.0 6 446 12 1 1 1 173 672 0.0 6 663 8 1 174 887 0.0 6 860 27 175 1263 0.0 6 1239 23 1 176 1068 0.0 6 1043 23 1 0 1 177 828 0.0 6 804 20 2 0 2 178 735 0.0 6 720 15 179 836 0.0 6 815 20 1 180 787 0.0 6 764 23 181 969 0.0 6 941 26 1 1 182 637 0.0 6 614 19 2 0 2 183 1056 0.0 6 1029 27 184 305 0.0 6 292 9 1 3 185 337 0.0 6 324 12 1 186 1154 0.0 6 1127 26 0 0 0 1 187 1466 0.0 6 1419 45 0 1 1 188 1875 0.0 6 1813 55 4 1 2 189 1190 0.0 6 1138 48 3 0 0 1 190 2153 0.0 6 2104 45 2 1 1 191 1721 0.0 6 1668 51 0 0 2 192 1382 0.0 6 1345 31 3 0 2 0 1 193 903 0.0 6 864 33 2 0 3 0 1 194 1335 0.0 6 1294 39 2 195 1414 0.0 6 1363 48 1 0 2 196 2992 0.0 6 2904 86 0 1 1 197 1225 0.0 6 1179 40 5 0 1 198 1752 0.0 6 1678 69 2 0 1 2 199 3680 0.0 6 3582 89 3 0 5 1 200 1063 0.0 6 1033 28 0 0 2 201 1807 0.0 6 1757 49 1 202 936 0.0 6 904 30 0 0 2 203 585 0.0 6 563 21 0 0 1 204 1213 0.0 6 1189 22 1 0 1 205 313 0.0 6 303 10 206 486 0.0 6 479 7 207 283 0.0 6 276 7 208 438 0.0 6 424 11 2 0 1 209 547 0.0 6 526 19 1 1 210 612 0.0 6 583 29 211 918 0.0 6 887 28 1 1 1 212 1138 0.0 6 1113 24 1 213 312 0.0 6 298 14 214 1096 0.0 6 1070 23 2 0 1 215 349 0.0 6 333 14 1 1 216 570 0.0 6 555 14 0 0 1 217 912 0.0 6 885 26 0 0 1 218 430 0.0 6 423 7 219 577 0.0 6 564 13 220 309 0.0 6 296 12 0 0 1 221 579 0.0 6 563 16 222 215 0.0 6 207 6 0 0 2 223 343 0.0 6 332 11 224 340 0.0 6 326 13 1 225 583 0.0 6 574 9 226 690 0.0 6 666 23 1 227 474 0.0 6 463 11 228 240 0.0 6 232 8 229 357 0.0 6 348 8 0 1 230 178 0.0 6 171 6 1 231 260 0.0 6 255 5 232 286 0.0 6 281 5 233 351 0.0 6 345 6 234 143 0.0 6 140 3 235 134 0.0 6 132 2 236 57 0.0 6 54 3 237 96 0.0 6 94 2 238 30 0.0 6 28 2 239 29 0.0 6 28 1 240 83 0.0 6 81 2 241 46 0.0 6 45 1 242 162 0.0 6 157 5 243 253 0.0 6 245 8 244 217 0.0 6 212 5 245 114 0.0 6 112 2 246 144 0.0 6 140 3 1 247 19 0.0 6 18 1 248 57 0.0 6 57 249 38 0.0 6 38 250 19 0.0 6 19 251 60 0.0 6 60 252 8 0.0 6 8 253 9 0.0 6 9 254 32 0.0 6 32 255 5 0.0 6 5 256 7 0.0 6 7 257 9 0.0 6 9 258 3 0.0 6 3 259 1 0.0 6 1 260 2 0.0 6 2 263 1 0.0 6 1 280 1 0.0 6 1 281 7 0.0 6 6 1 282 37 0.0 6 33 2 1 0 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 565 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 50 1 0.0 6 1 52 1 0.0 6 1 60 9 0.0 6 9 68 1 0.0 6 1 69 2 0.0 6 2 70 4 0.0 6 4 71 11 0.0 6 11 72 2 0.0 6 2 73 6 0.0 6 6 74 7 0.0 6 7 75 10 0.0 6 10 76 53 0.0 6 53 77 8 0.0 6 8 78 4 0.0 6 4 79 9 0.0 6 8 0 0 1 80 6 0.0 6 5 0 0 0 1 81 4 0.0 6 4 82 6 0.0 6 6 83 9 0.0 6 9 84 3 0.0 6 3 85 1 0.0 6 1 86 4 0.0 6 4 87 3 0.0 6 3 88 4 0.0 6 4 89 11 0.0 6 11 90 10 0.0 6 10 91 7 0.0 6 7 92 3 0.0 6 3 93 5 0.0 6 5 94 2 0.0 6 2 95 5 0.0 6 5 96 6 0.0 6 6 97 3 0.0 6 3 98 4 0.0 6 4 99 8 0.0 6 8 100 12 0.0 6 12 101 4 0.0 6 4 102 5 0.0 6 4 0 0 0 1 103 7 0.0 6 7 104 3 0.0 6 3 105 4 0.0 6 4 106 6 0.0 6 6 107 13 0.0 6 13 108 5 0.0 6 5 109 3 0.0 6 3 110 3 0.0 6 3 111 2 0.0 6 2 112 2 0.0 6 2 113 6 0.0 6 6 114 7 0.0 6 7 115 2 0.0 6 2 116 4 0.0 6 4 117 8 0.0 6 8 118 6 0.0 6 6 119 2 0.0 6 2 120 2 0.0 6 1 0 0 1 121 8 0.0 6 8 122 2 0.0 6 2 123 3 0.0 6 3 124 2 0.0 6 2 125 11 0.0 6 11 126 4 0.0 6 4 127 6 0.0 6 6 128 3 0.0 6 3 129 5 0.0 6 5 130 5 0.0 6 5 131 12 0.0 6 12 132 1 0.0 6 1 133 5 0.0 6 5 134 8 0.0 6 8 135 3 0.0 6 3 136 3 0.0 6 3 137 6 0.0 6 6 138 4 0.0 6 4 140 2 0.0 6 2 141 5 0.0 6 5 142 6 0.0 6 6 143 5 0.0 6 5 144 2 0.0 6 2 145 4 0.0 6 4 146 3 0.0 6 3 147 2 0.0 6 2 148 3 0.0 6 3 149 3 0.0 6 3 150 4 0.0 6 4 151 4 0.0 6 4 152 4 0.0 6 4 153 2 0.0 6 2 154 2 0.0 6 2 155 4 0.0 6 4 156 4 0.0 6 4 157 1 0.0 6 1 158 7 0.0 6 7 159 3 0.0 6 3 160 5 0.0 6 5 161 3 0.0 6 3 162 3 0.0 6 3 163 1 0.0 6 1 165 1 0.0 6 1 167 2 0.0 6 2 169 1 0.0 6 1 170 1 0.0 6 1 171 1 0.0 6 1 172 2 0.0 6 2 173 3 0.0 6 3 174 3 0.0 6 3 175 2 0.0 6 2 178 1 0.0 6 1 179 4 0.0 6 4 182 2 0.0 6 2 183 5 0.0 6 5 184 2 0.0 6 2 185 5 0.0 6 5 186 3 0.0 6 3 188 1 0.0 6 1 189 2 0.0 6 2 190 1 0.0 6 1 191 1 0.0 6 1 194 3 0.0 6 3 195 1 0.0 6 1 197 1 0.0 6 1 199 1 0.0 6 1 200 1 0.0 6 1 215 2 0.0 6 2 225 1 0.0 6 1 250 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_18_69_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_18_296_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_18_69_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_18_296_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.14 s (32 µs/read; 1.86 M reads/minute). === Summary === Total read pairs processed: 66,046 Read 1 with adapter: 66,039 (100.0%) Read 2 with adapter: 65,995 (99.9%) == Read fate breakdown == Pairs that were too short: 26 (0.0%) Pairs discarded as untrimmed: 58 (0.1%) Pairs written (passing filters): 65,962 (99.9%) Total basepairs processed: 28,375,536 bp Read 1: 15,748,526 bp Read 2: 12,627,010 bp Total written (filtered): 4,227,281 bp (14.9%) Read 1: 2,059,239 bp Read 2: 2,168,042 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 64485 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1032.0 0 1 1 4 3 258.0 1 2 1 5 1 64.5 1 1 6 2 16.1 1 0 0 2 7 2 4.0 2 1 0 1 8 1 1.0 2 1 9 2 0.3 2 2 16 2 0.0 4 1 1 18 2 0.0 5 2 19 4 0.0 5 1 3 20 140 0.0 6 137 3 21 71 0.0 6 68 3 22 74 0.0 6 71 3 23 41 0.0 6 41 24 47 0.0 6 44 1 0 0 2 25 27 0.0 6 26 1 26 18 0.0 6 17 1 27 12 0.0 6 12 28 18 0.0 6 18 29 15 0.0 6 14 0 0 1 30 8 0.0 6 8 31 9 0.0 6 8 1 32 4 0.0 6 4 33 7 0.0 6 6 0 0 0 1 34 8 0.0 6 8 35 9 0.0 6 9 36 6 0.0 6 6 37 7 0.0 6 7 38 5 0.0 6 5 39 13 0.0 6 12 1 40 11 0.0 6 11 41 7 0.0 6 7 42 9 0.0 6 8 1 43 6 0.0 6 6 44 6 0.0 6 6 45 4 0.0 6 4 46 5 0.0 6 5 47 6 0.0 6 6 48 6 0.0 6 6 49 9 0.0 6 9 50 3 0.0 6 2 1 51 9 0.0 6 9 52 4 0.0 6 4 53 6 0.0 6 6 54 10 0.0 6 10 55 6 0.0 6 6 56 5 0.0 6 5 57 7 0.0 6 7 58 5 0.0 6 5 59 5 0.0 6 5 60 6 0.0 6 6 61 6 0.0 6 6 62 7 0.0 6 7 63 5 0.0 6 4 1 64 4 0.0 6 4 65 2 0.0 6 2 66 10 0.0 6 9 1 67 4 0.0 6 3 1 68 5 0.0 6 5 69 6 0.0 6 6 70 7 0.0 6 7 71 8 0.0 6 8 72 10 0.0 6 9 1 73 4 0.0 6 4 74 17 0.0 6 16 1 75 8 0.0 6 7 1 76 12 0.0 6 11 1 77 10 0.0 6 10 78 3 0.0 6 3 79 12 0.0 6 10 2 80 8 0.0 6 8 81 12 0.0 6 12 82 4 0.0 6 4 83 9 0.0 6 8 0 0 1 84 6 0.0 6 6 85 14 0.0 6 14 86 9 0.0 6 8 0 0 0 0 0 1 87 14 0.0 6 14 88 5 0.0 6 5 89 18 0.0 6 16 2 90 15 0.0 6 14 1 91 6 0.0 6 6 92 6 0.0 6 6 93 2 0.0 6 2 94 9 0.0 6 9 95 7 0.0 6 6 1 96 4 0.0 6 3 0 0 1 97 16 0.0 6 16 98 8 0.0 6 8 99 5 0.0 6 5 100 12 0.0 6 11 1 101 10 0.0 6 10 102 10 0.0 6 10 103 17 0.0 6 17 104 15 0.0 6 15 105 18 0.0 6 17 1 106 19 0.0 6 18 1 107 21 0.0 6 20 1 108 14 0.0 6 13 1 109 18 0.0 6 15 3 110 16 0.0 6 16 111 13 0.0 6 13 112 19 0.0 6 18 1 113 21 0.0 6 21 114 19 0.0 6 16 3 115 20 0.0 6 20 116 18 0.0 6 18 117 18 0.0 6 18 118 24 0.0 6 24 119 15 0.0 6 15 120 22 0.0 6 20 2 121 26 0.0 6 26 122 27 0.0 6 26 1 123 30 0.0 6 30 124 33 0.0 6 32 1 125 23 0.0 6 21 2 126 29 0.0 6 29 127 31 0.0 6 29 0 0 0 0 1 1 128 29 0.0 6 29 129 32 0.0 6 32 130 37 0.0 6 36 1 131 33 0.0 6 33 132 26 0.0 6 26 133 34 0.0 6 34 134 29 0.0 6 27 2 135 36 0.0 6 34 2 136 49 0.0 6 48 1 137 39 0.0 6 37 2 138 55 0.0 6 54 1 139 224 0.0 6 220 3 0 1 140 116 0.0 6 113 2 0 1 141 53 0.0 6 52 1 142 75 0.0 6 75 143 212 0.0 6 212 144 272 0.0 6 271 1 145 370 0.0 6 365 3 1 0 0 0 1 146 516 0.0 6 516 147 227 0.0 6 227 148 571 0.0 6 571 149 273 0.0 6 273 150 222 0.0 6 219 3 151 431 0.0 6 429 2 152 265 0.0 6 262 3 153 229 0.0 6 228 1 154 369 0.0 6 369 155 262 0.0 6 261 1 156 198 0.0 6 196 2 157 216 0.0 6 215 1 158 268 0.0 6 268 159 313 0.0 6 313 160 632 0.0 6 632 161 332 0.0 6 330 2 162 252 0.0 6 250 1 0 0 0 1 163 306 0.0 6 306 164 561 0.0 6 560 0 0 0 0 0 1 165 231 0.0 6 231 166 567 0.0 6 566 1 167 268 0.0 6 267 1 168 232 0.0 6 231 1 169 280 0.0 6 277 3 170 326 0.0 6 324 2 171 226 0.0 6 224 2 172 211 0.0 6 210 1 173 268 0.0 6 265 3 174 390 0.0 6 390 175 327 0.0 6 324 2 0 0 0 0 1 176 264 0.0 6 262 2 177 358 0.0 6 357 1 178 560 0.0 6 558 2 179 348 0.0 6 347 0 0 0 0 1 180 441 0.0 6 438 3 181 1168 0.0 6 1166 2 182 418 0.0 6 415 2 0 0 0 1 183 432 0.0 6 432 184 304 0.0 6 303 0 1 185 230 0.0 6 228 1 0 1 186 253 0.0 6 253 187 385 0.0 6 382 3 188 660 0.0 6 659 1 189 345 0.0 6 345 190 378 0.0 6 377 1 191 339 0.0 6 336 3 192 335 0.0 6 332 2 1 193 407 0.0 6 403 4 194 538 0.0 6 535 2 1 195 518 0.0 6 516 2 196 579 0.0 6 577 2 197 581 0.0 6 576 4 0 0 0 1 198 682 0.0 6 677 5 199 873 0.0 6 867 5 0 0 1 200 687 0.0 6 681 6 201 699 0.0 6 698 1 202 612 0.0 6 603 7 0 0 0 1 1 203 559 0.0 6 557 2 204 513 0.0 6 511 2 205 766 0.0 6 761 5 206 572 0.0 6 569 3 207 594 0.0 6 590 3 0 1 208 713 0.0 6 708 5 209 609 0.0 6 609 210 750 0.0 6 746 4 211 598 0.0 6 592 5 1 212 610 0.0 6 607 2 0 1 213 718 0.0 6 713 4 0 1 214 725 0.0 6 718 6 0 0 0 0 1 215 554 0.0 6 550 4 216 740 0.0 6 736 4 217 958 0.0 6 952 6 218 709 0.0 6 703 6 219 744 0.0 6 739 4 0 0 0 0 1 220 711 0.0 6 705 5 0 0 0 0 1 221 726 0.0 6 721 5 222 642 0.0 6 640 2 223 608 0.0 6 605 3 224 585 0.0 6 582 1 0 0 1 1 225 698 0.0 6 692 6 226 771 0.0 6 765 5 0 1 227 784 0.0 6 778 6 228 757 0.0 6 752 5 229 595 0.0 6 594 1 230 559 0.0 6 557 2 231 531 0.0 6 529 2 232 714 0.0 6 707 7 233 660 0.0 6 656 4 234 708 0.0 6 707 0 1 235 599 0.0 6 596 2 1 236 567 0.0 6 566 1 237 469 0.0 6 469 238 514 0.0 6 511 3 239 472 0.0 6 468 4 240 544 0.0 6 539 4 0 0 0 1 241 444 0.0 6 443 1 242 511 0.0 6 508 1 1 0 0 0 1 243 346 0.0 6 344 2 244 404 0.0 6 401 3 245 389 0.0 6 387 2 246 384 0.0 6 382 2 247 343 0.0 6 338 5 248 219 0.0 6 218 0 1 249 245 0.0 6 244 1 250 244 0.0 6 241 3 251 260 0.0 6 258 2 252 277 0.0 6 274 3 253 207 0.0 6 206 1 254 219 0.0 6 215 3 1 255 240 0.0 6 236 4 256 199 0.0 6 195 4 257 208 0.0 6 206 2 258 191 0.0 6 189 2 259 233 0.0 6 232 1 260 208 0.0 6 206 2 261 243 0.0 6 236 7 262 178 0.0 6 176 2 263 212 0.0 6 207 5 264 186 0.0 6 184 2 265 180 0.0 6 177 3 266 145 0.0 6 144 1 267 172 0.0 6 170 2 268 127 0.0 6 126 1 269 121 0.0 6 117 3 0 0 0 0 1 270 141 0.0 6 141 271 134 0.0 6 130 4 272 152 0.0 6 148 3 1 273 101 0.0 6 100 1 274 112 0.0 6 107 5 275 172 0.0 6 170 2 276 279 0.0 6 275 4 277 266 0.0 6 257 9 278 325 0.0 6 320 5 279 442 0.0 6 436 5 1 280 993 0.0 6 971 20 1 0 1 281 2920 0.0 6 2875 45 282 7 0.0 6 5 2 284 1 0.0 6 1 285 3 0.0 6 3 288 1 0.0 6 1 293 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1554 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 1 0.0 6 0 1 61 20 0.0 6 19 1 69 1 0.0 6 1 70 2 0.0 6 2 71 15 0.0 6 15 72 14 0.0 6 14 73 13 0.0 6 13 74 12 0.0 6 12 75 16 0.0 6 16 76 3 0.0 6 3 77 10 0.0 6 10 78 12 0.0 6 12 79 8 0.0 6 8 80 3 0.0 6 3 81 10 0.0 6 10 82 9 0.0 6 9 83 9 0.0 6 9 84 9 0.0 6 9 85 4 0.0 6 4 86 13 0.0 6 13 87 16 0.0 6 16 88 2 0.0 6 2 89 8 0.0 6 8 90 5 0.0 6 5 91 15 0.0 6 15 92 9 0.0 6 9 93 15 0.0 6 15 94 4 0.0 6 4 96 7 0.0 6 7 97 8 0.0 6 8 98 2 0.0 6 2 99 5 0.0 6 5 100 8 0.0 6 8 101 10 0.0 6 10 102 7 0.0 6 7 103 4 0.0 6 4 104 9 0.0 6 9 105 18 0.0 6 18 106 10 0.0 6 9 1 107 23 0.0 6 23 108 35 0.0 6 35 109 6 0.0 6 6 110 3 0.0 6 3 111 6 0.0 6 6 112 3 0.0 6 3 113 4 0.0 6 4 114 16 0.0 6 16 115 15 0.0 6 15 116 3 0.0 6 3 117 15 0.0 6 15 118 5 0.0 6 5 119 4 0.0 6 4 120 2 0.0 6 2 121 3 0.0 6 3 122 3 0.0 6 3 123 6 0.0 6 6 124 14 0.0 6 14 125 13 0.0 6 12 0 0 0 0 0 1 126 12 0.0 6 12 127 4 0.0 6 4 128 11 0.0 6 11 129 9 0.0 6 9 130 5 0.0 6 5 131 4 0.0 6 4 132 9 0.0 6 9 133 7 0.0 6 7 134 7 0.0 6 7 135 5 0.0 6 5 136 6 0.0 6 6 137 15 0.0 6 15 138 11 0.0 6 11 139 10 0.0 6 10 140 5 0.0 6 5 141 5 0.0 6 5 142 4 0.0 6 4 143 4 0.0 6 4 144 2 0.0 6 2 145 2 0.0 6 2 146 9 0.0 6 9 147 8 0.0 6 8 148 9 0.0 6 9 149 15 0.0 6 15 150 4 0.0 6 4 151 4 0.0 6 4 152 13 0.0 6 13 153 19 0.0 6 19 154 13 0.0 6 13 155 15 0.0 6 15 156 19 0.0 6 19 157 5 0.0 6 5 158 17 0.0 6 17 159 21 0.0 6 21 160 8 0.0 6 8 161 10 0.0 6 10 162 10 0.0 6 10 163 10 0.0 6 10 164 22 0.0 6 22 165 27 0.0 6 27 166 17 0.0 6 17 167 17 0.0 6 17 168 8 0.0 6 7 0 0 0 0 0 1 169 14 0.0 6 14 170 6 0.0 6 6 171 6 0.0 6 6 172 16 0.0 6 16 173 22 0.0 6 22 174 21 0.0 6 21 175 5 0.0 6 5 176 6 0.0 6 6 177 13 0.0 6 13 178 15 0.0 6 15 179 13 0.0 6 13 180 7 0.0 6 7 181 11 0.0 6 11 182 13 0.0 6 13 183 13 0.0 6 13 184 5 0.0 6 5 185 11 0.0 6 11 186 5 0.0 6 5 187 5 0.0 6 5 188 9 0.0 6 9 189 7 0.0 6 7 190 6 0.0 6 6 191 4 0.0 6 4 192 18 0.0 6 18 193 13 0.0 6 13 194 13 0.0 6 13 195 6 0.0 6 6 196 9 0.0 6 9 197 8 0.0 6 8 198 2 0.0 6 2 199 6 0.0 6 6 200 12 0.0 6 12 201 13 0.0 6 13 202 14 0.0 6 14 203 6 0.0 6 6 204 4 0.0 6 4 205 6 0.0 6 6 206 11 0.0 6 11 207 7 0.0 6 7 208 5 0.0 6 5 209 10 0.0 6 10 210 9 0.0 6 9 211 12 0.0 6 12 212 5 0.0 6 5 213 2 0.0 6 2 214 4 0.0 6 4 215 3 0.0 6 3 216 6 0.0 6 6 218 6 0.0 6 6 219 1 0.0 6 1 220 3 0.0 6 3 221 6 0.0 6 6 222 8 0.0 6 8 223 4 0.0 6 4 224 6 0.0 6 6 225 11 0.0 6 11 226 2 0.0 6 2 227 1 0.0 6 1 228 1 0.0 6 1 229 5 0.0 6 5 230 4 0.0 6 4 231 3 0.0 6 3 232 1 0.0 6 1 234 6 0.0 6 6 235 6 0.0 6 6 237 2 0.0 6 2 238 2 0.0 6 2 239 3 0.0 6 3 240 4 0.0 6 4 241 2 0.0 6 2 242 6 0.0 6 6 243 6 0.0 6 6 244 2 0.0 6 2 246 4 0.0 6 4 247 2 0.0 6 2 248 4 0.0 6 4 250 2 0.0 6 2 251 3 0.0 6 3 252 3 0.0 6 3 253 1 0.0 6 1 254 5 0.0 6 5 255 6 0.0 6 6 256 3 0.0 6 3 257 1 0.0 6 1 258 1 0.0 6 1 259 1 0.0 6 1 262 2 0.0 6 2 263 2 0.0 6 2 264 2 0.0 6 2 267 2 0.0 6 2 268 1 0.0 6 1 276 2 0.0 6 2 277 2 0.0 6 2 280 1 0.0 6 1 287 2 0.0 6 2 290 1 0.0 6 1 308 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 64276 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 1032.0 0 4 4 1 258.0 1 1 6 1 16.1 1 1 8 1 1.0 2 1 9 1 0.3 2 1 10 2 0.1 3 2 11 3 0.0 3 3 12 1 0.0 3 1 13 4 0.0 3 4 14 6 0.0 4 6 16 13 0.0 4 13 17 30 0.0 5 26 4 18 15 0.0 5 12 3 19 16 0.0 5 14 2 20 114 0.0 6 108 5 1 21 58 0.0 6 57 1 22 38 0.0 6 36 2 23 32 0.0 6 31 1 24 21 0.0 6 21 25 24 0.0 6 21 3 26 23 0.0 6 22 1 27 14 0.0 6 14 28 23 0.0 6 20 3 29 13 0.0 6 12 1 30 18 0.0 6 18 31 10 0.0 6 8 1 0 0 1 32 19 0.0 6 17 2 33 17 0.0 6 13 4 34 8 0.0 6 7 1 35 19 0.0 6 16 3 36 12 0.0 6 12 37 13 0.0 6 13 38 15 0.0 6 15 39 21 0.0 6 17 4 40 12 0.0 6 11 1 41 12 0.0 6 12 42 14 0.0 6 12 1 0 0 1 43 15 0.0 6 13 1 1 44 17 0.0 6 16 1 45 20 0.0 6 16 2 1 0 0 1 46 16 0.0 6 15 1 47 12 0.0 6 12 48 18 0.0 6 15 3 49 24 0.0 6 23 1 50 15 0.0 6 14 1 51 22 0.0 6 18 2 1 0 0 1 52 24 0.0 6 22 1 1 53 27 0.0 6 25 2 54 17 0.0 6 15 2 55 16 0.0 6 16 56 18 0.0 6 16 2 57 25 0.0 6 19 5 0 0 0 0 1 58 31 0.0 6 31 59 26 0.0 6 25 1 60 24 0.0 6 23 1 61 16 0.0 6 14 2 62 19 0.0 6 18 1 63 32 0.0 6 31 1 64 30 0.0 6 27 3 65 22 0.0 6 20 1 1 66 49 0.0 6 47 2 67 99 0.0 6 94 3 1 0 1 68 61 0.0 6 56 5 69 81 0.0 6 75 5 1 70 34 0.0 6 32 2 71 43 0.0 6 42 1 72 28 0.0 6 27 1 73 32 0.0 6 28 4 74 27 0.0 6 27 75 31 0.0 6 29 2 76 37 0.0 6 36 1 77 38 0.0 6 35 2 0 1 78 34 0.0 6 32 2 79 44 0.0 6 44 80 57 0.0 6 55 2 81 41 0.0 6 40 1 82 44 0.0 6 43 1 83 69 0.0 6 65 4 84 36 0.0 6 35 1 85 44 0.0 6 43 1 86 38 0.0 6 37 1 87 50 0.0 6 49 1 88 35 0.0 6 32 3 89 47 0.0 6 45 2 90 57 0.0 6 55 2 91 61 0.0 6 60 1 92 59 0.0 6 55 4 93 64 0.0 6 61 2 0 1 94 58 0.0 6 55 2 0 0 0 1 95 77 0.0 6 75 2 96 81 0.0 6 77 4 97 55 0.0 6 51 3 0 0 0 1 98 57 0.0 6 54 3 99 61 0.0 6 61 100 85 0.0 6 82 2 1 101 104 0.0 6 100 4 102 82 0.0 6 76 4 1 0 1 103 119 0.0 6 113 6 104 172 0.0 6 162 9 0 0 0 0 1 105 94 0.0 6 90 3 1 106 80 0.0 6 78 1 1 107 94 0.0 6 88 4 2 108 91 0.0 6 86 4 1 109 100 0.0 6 99 0 1 110 116 0.0 6 111 5 111 132 0.0 6 126 4 1 0 1 112 136 0.0 6 129 6 0 0 0 0 1 113 125 0.0 6 123 2 114 104 0.0 6 99 4 0 0 0 0 1 115 140 0.0 6 137 3 116 116 0.0 6 109 5 1 0 0 1 117 143 0.0 6 140 3 118 164 0.0 6 157 7 119 157 0.0 6 153 3 0 0 0 1 120 135 0.0 6 134 1 121 160 0.0 6 158 1 0 0 0 0 1 122 163 0.0 6 159 3 1 123 195 0.0 6 190 5 124 233 0.0 6 226 4 0 0 2 0 1 125 248 0.0 6 242 4 2 126 216 0.0 6 211 3 2 127 244 0.0 6 236 8 128 248 0.0 6 237 11 129 339 0.0 6 332 7 130 287 0.0 6 277 7 0 0 1 0 2 131 354 0.0 6 347 6 0 0 1 132 358 0.0 6 350 6 1 0 0 1 133 394 0.0 6 388 6 134 431 0.0 6 422 8 0 1 135 431 0.0 6 422 7 0 0 1 0 1 136 402 0.0 6 398 4 137 410 0.0 6 399 11 138 551 0.0 6 541 8 0 1 0 1 139 600 0.0 6 589 8 1 0 0 0 2 140 640 0.0 6 630 9 1 141 837 0.0 6 827 10 142 743 0.0 6 728 12 0 0 1 0 2 143 955 0.0 6 939 13 2 0 1 144 751 0.0 6 744 6 0 1 145 742 0.0 6 734 5 0 0 2 0 1 146 939 0.0 6 924 13 0 0 0 1 1 147 800 0.0 6 792 7 0 0 0 0 1 148 899 0.0 6 882 16 0 0 0 0 1 149 1084 0.0 6 1067 15 1 0 1 150 1358 0.0 6 1338 18 0 0 2 151 1044 0.0 6 1033 9 1 0 0 0 1 152 1266 0.0 6 1253 11 2 153 1282 0.0 6 1264 13 1 1 1 2 154 1033 0.0 6 1024 8 0 1 155 1270 0.0 6 1258 9 1 0 0 0 2 156 971 0.0 6 959 12 157 1070 0.0 6 1057 9 2 2 158 1096 0.0 6 1087 7 1 0 0 1 159 1083 0.0 6 1074 7 0 1 0 0 1 160 925 0.0 6 904 18 1 0 1 1 161 943 0.0 6 933 9 0 1 162 710 0.0 6 699 6 3 0 0 1 1 163 744 0.0 6 734 8 0 0 0 1 1 164 811 0.0 6 795 14 1 0 0 1 165 778 0.0 6 766 11 0 0 0 1 166 942 0.0 6 932 9 0 0 0 0 1 167 821 0.0 6 812 7 0 1 1 168 1021 0.0 6 1014 6 0 0 0 1 169 803 0.0 6 800 3 170 878 0.0 6 868 7 1 0 0 1 1 171 1006 0.0 6 988 16 0 1 0 0 1 172 987 0.0 6 976 11 173 1077 0.0 6 1065 12 174 943 0.0 6 936 7 175 945 0.0 6 935 8 0 1 0 1 176 1079 0.0 6 1072 6 0 1 177 774 0.0 6 767 7 178 735 0.0 6 730 5 179 613 0.0 6 609 3 0 0 1 180 568 0.0 6 560 8 181 544 0.0 6 538 6 182 529 0.0 6 523 5 0 1 183 830 0.0 6 823 6 1 184 920 0.0 6 913 5 1 0 1 185 731 0.0 6 720 10 1 186 622 0.0 6 612 9 0 0 0 1 187 598 0.0 6 591 5 0 1 1 188 527 0.0 6 524 2 1 189 514 0.0 6 510 4 190 516 0.0 6 504 10 0 0 1 1 191 593 0.0 6 586 6 1 192 346 0.0 6 344 2 193 345 0.0 6 342 3 194 349 0.0 6 344 5 195 354 0.0 6 348 4 1 0 1 196 516 0.0 6 513 2 0 1 197 343 0.0 6 339 4 198 384 0.0 6 379 5 199 429 0.0 6 416 10 1 2 200 282 0.0 6 280 1 1 201 291 0.0 6 285 4 0 0 1 0 1 202 241 0.0 6 238 3 203 220 0.0 6 215 5 204 234 0.0 6 230 3 0 0 1 205 196 0.0 6 195 1 206 165 0.0 6 165 207 184 0.0 6 180 3 1 208 189 0.0 6 187 2 209 112 0.0 6 110 0 1 0 1 210 118 0.0 6 117 1 211 144 0.0 6 139 5 212 114 0.0 6 114 213 92 0.0 6 90 1 0 0 0 0 1 214 158 0.0 6 154 4 215 128 0.0 6 124 4 216 157 0.0 6 154 3 217 98 0.0 6 96 1 1 218 72 0.0 6 72 219 70 0.0 6 68 2 220 44 0.0 6 43 1 221 102 0.0 6 102 222 104 0.0 6 102 2 223 118 0.0 6 117 1 224 32 0.0 6 31 1 225 54 0.0 6 52 2 226 53 0.0 6 53 227 67 0.0 6 63 4 228 61 0.0 6 59 2 229 69 0.0 6 66 2 0 0 1 230 167 0.0 6 165 2 231 49 0.0 6 47 2 232 115 0.0 6 112 3 233 26 0.0 6 25 1 234 26 0.0 6 26 235 19 0.0 6 19 236 17 0.0 6 16 1 237 13 0.0 6 13 238 49 0.0 6 49 239 13 0.0 6 13 240 16 0.0 6 16 241 25 0.0 6 25 242 26 0.0 6 26 243 16 0.0 6 16 244 15 0.0 6 15 245 20 0.0 6 20 246 18 0.0 6 18 247 6 0.0 6 6 248 11 0.0 6 10 1 249 4 0.0 6 4 250 6 0.0 6 5 1 251 16 0.0 6 14 2 252 5 0.0 6 5 257 1 0.0 6 1 275 2 0.0 6 2 277 1 0.0 6 1 278 10 0.0 6 10 279 43 0.0 6 42 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1719 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 55 1 0.0 6 1 64 1 0.0 6 0 0 0 0 1 67 7 0.0 6 3 4 68 329 0.0 6 323 6 69 1 0.0 6 1 70 1 0.0 6 1 71 3 0.0 6 3 72 8 0.0 6 8 73 12 0.0 6 12 74 7 0.0 6 7 75 11 0.0 6 11 76 11 0.0 6 11 77 19 0.0 6 19 78 9 0.0 6 9 79 10 0.0 6 10 80 14 0.0 6 14 81 7 0.0 6 7 82 9 0.0 6 8 1 83 17 0.0 6 17 84 9 0.0 6 9 85 8 0.0 6 8 86 8 0.0 6 8 87 7 0.0 6 7 88 18 0.0 6 18 89 14 0.0 6 14 90 5 0.0 6 5 91 8 0.0 6 8 92 8 0.0 6 8 93 18 0.0 6 18 94 9 0.0 6 9 95 25 0.0 6 25 96 11 0.0 6 11 97 5 0.0 6 5 98 9 0.0 6 9 99 13 0.0 6 13 100 9 0.0 6 8 1 101 4 0.0 6 4 102 10 0.0 6 10 103 5 0.0 6 5 104 6 0.0 6 6 105 4 0.0 6 4 106 11 0.0 6 11 107 11 0.0 6 11 108 5 0.0 6 5 109 21 0.0 6 21 110 49 0.0 6 48 0 0 0 1 111 10 0.0 6 9 1 112 11 0.0 6 11 113 6 0.0 6 6 114 7 0.0 6 6 0 0 0 1 115 3 0.0 6 3 116 10 0.0 6 10 117 18 0.0 6 18 118 14 0.0 6 14 119 13 0.0 6 13 120 8 0.0 6 8 121 5 0.0 6 5 122 9 0.0 6 9 123 12 0.0 6 12 124 6 0.0 6 6 125 12 0.0 6 12 126 9 0.0 6 9 127 17 0.0 6 17 128 13 0.0 6 13 129 6 0.0 6 6 130 14 0.0 6 14 131 6 0.0 6 6 132 7 0.0 6 7 133 5 0.0 6 5 134 13 0.0 6 13 135 5 0.0 6 5 136 3 0.0 6 3 137 6 0.0 6 6 138 7 0.0 6 7 139 16 0.0 6 16 140 12 0.0 6 12 141 12 0.0 6 12 142 6 0.0 6 6 143 3 0.0 6 3 144 1 0.0 6 1 145 9 0.0 6 9 146 2 0.0 6 2 147 3 0.0 6 3 148 9 0.0 6 9 149 12 0.0 6 12 150 7 0.0 6 6 1 151 13 0.0 6 13 152 9 0.0 6 8 0 0 0 0 1 153 4 0.0 6 4 154 8 0.0 6 8 155 23 0.0 6 23 156 16 0.0 6 16 157 17 0.0 6 17 158 22 0.0 6 22 159 9 0.0 6 9 160 7 0.0 6 7 161 22 0.0 6 22 162 12 0.0 6 12 163 5 0.0 6 5 164 19 0.0 6 19 165 14 0.0 6 14 166 16 0.0 6 16 167 38 0.0 6 38 168 21 0.0 6 21 169 11 0.0 6 11 170 12 0.0 6 12 171 15 0.0 6 15 172 7 0.0 6 7 173 12 0.0 6 12 174 16 0.0 6 16 175 21 0.0 6 21 176 23 0.0 6 23 177 5 0.0 6 5 178 6 0.0 6 6 179 8 0.0 6 8 180 22 0.0 6 22 181 9 0.0 6 9 182 8 0.0 6 8 183 7 0.0 6 7 184 10 0.0 6 10 185 3 0.0 6 2 1 186 2 0.0 6 2 187 8 0.0 6 8 188 1 0.0 6 1 189 2 0.0 6 2 190 7 0.0 6 7 191 3 0.0 6 3 192 4 0.0 6 4 193 1 0.0 6 1 194 7 0.0 6 7 195 6 0.0 6 6 196 4 0.0 6 4 197 8 0.0 6 8 198 8 0.0 6 7 1 199 5 0.0 6 5 201 3 0.0 6 3 202 2 0.0 6 2 203 4 0.0 6 4 204 4 0.0 6 4 205 6 0.0 6 6 206 4 0.0 6 4 207 2 0.0 6 2 208 1 0.0 6 1 209 2 0.0 6 2 210 1 0.0 6 1 211 1 0.0 6 1 212 2 0.0 6 2 213 2 0.0 6 2 214 2 0.0 6 2 215 1 0.0 6 1 216 1 0.0 6 1 217 1 0.0 6 1 218 1 0.0 6 1 226 4 0.0 6 4 227 1 0.0 6 1 228 1 0.0 6 1 237 1 0.0 6 1 245 1 0.0 6 1 275 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_19_70_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_19_297_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_19_70_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_19_297_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.67 s (31 µs/read; 1.94 M reads/minute). === Summary === Total read pairs processed: 54,132 Read 1 with adapter: 54,128 (100.0%) Read 2 with adapter: 54,102 (99.9%) == Read fate breakdown == Pairs that were too short: 3 (0.0%) Pairs discarded as untrimmed: 34 (0.1%) Pairs written (passing filters): 54,095 (99.9%) Total basepairs processed: 26,894,558 bp Read 1: 15,467,381 bp Read 2: 11,427,177 bp Total written (filtered): 3,257,826 bp (12.1%) Read 1: 1,629,543 bp Read 2: 1,628,283 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 54017 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 845.8 0 0 1 4 1 211.5 1 0 1 6 2 13.2 1 0 0 2 8 1 0.8 2 1 9 2 0.2 2 1 1 10 1 0.1 3 0 1 11 1 0.0 3 1 12 1 0.0 3 1 15 1 0.0 4 1 16 3 0.0 4 2 1 19 2 0.0 5 1 1 20 17 0.0 6 16 1 21 8 0.0 6 7 1 22 6 0.0 6 6 23 6 0.0 6 6 24 2 0.0 6 2 25 6 0.0 6 6 26 3 0.0 6 3 27 5 0.0 6 4 1 28 4 0.0 6 4 29 3 0.0 6 3 30 2 0.0 6 2 31 1 0.0 6 1 32 1 0.0 6 1 33 3 0.0 6 2 1 34 6 0.0 6 5 1 35 1 0.0 6 1 38 2 0.0 6 2 39 2 0.0 6 1 1 40 1 0.0 6 1 42 1 0.0 6 1 44 4 0.0 6 4 45 5 0.0 6 5 47 1 0.0 6 1 49 1 0.0 6 1 52 2 0.0 6 2 53 1 0.0 6 1 54 3 0.0 6 3 55 2 0.0 6 1 1 56 4 0.0 6 4 57 3 0.0 6 1 2 58 1 0.0 6 0 1 60 3 0.0 6 3 62 3 0.0 6 3 63 5 0.0 6 5 65 1 0.0 6 1 69 3 0.0 6 3 70 2 0.0 6 2 71 1 0.0 6 1 72 2 0.0 6 2 74 1 0.0 6 1 77 4 0.0 6 4 78 1 0.0 6 1 82 1 0.0 6 1 83 1 0.0 6 1 86 13 0.0 6 13 87 3 0.0 6 3 89 3 0.0 6 3 90 2 0.0 6 2 91 1 0.0 6 0 1 92 5 0.0 6 5 93 3 0.0 6 1 2 94 2 0.0 6 2 96 1 0.0 6 0 1 97 4 0.0 6 4 98 3 0.0 6 3 100 6 0.0 6 6 101 4 0.0 6 4 102 8 0.0 6 8 103 4 0.0 6 4 104 2 0.0 6 2 105 5 0.0 6 5 106 4 0.0 6 4 107 9 0.0 6 9 108 5 0.0 6 4 1 109 2 0.0 6 1 1 110 3 0.0 6 3 111 3 0.0 6 3 112 3 0.0 6 3 113 5 0.0 6 5 114 5 0.0 6 5 115 4 0.0 6 3 1 116 7 0.0 6 7 117 7 0.0 6 7 118 3 0.0 6 3 119 6 0.0 6 6 120 4 0.0 6 4 121 10 0.0 6 9 1 122 8 0.0 6 8 123 8 0.0 6 7 1 124 1 0.0 6 1 125 23 0.0 6 23 126 10 0.0 6 9 0 1 127 7 0.0 6 7 128 24 0.0 6 23 1 129 7 0.0 6 7 130 1 0.0 6 1 131 5 0.0 6 5 132 12 0.0 6 12 133 11 0.0 6 11 134 10 0.0 6 9 1 135 22 0.0 6 21 1 136 12 0.0 6 12 137 34 0.0 6 32 1 1 138 23 0.0 6 21 1 0 0 1 139 12 0.0 6 12 140 24 0.0 6 23 0 1 141 46 0.0 6 46 142 85 0.0 6 85 143 115 0.0 6 115 144 71 0.0 6 71 145 63 0.0 6 62 1 146 72 0.0 6 71 1 147 54 0.0 6 53 1 148 84 0.0 6 82 2 149 79 0.0 6 78 1 150 68 0.0 6 67 1 151 82 0.0 6 81 1 152 37 0.0 6 37 153 75 0.0 6 75 154 77 0.0 6 77 155 51 0.0 6 50 1 156 44 0.0 6 44 157 47 0.0 6 47 158 58 0.0 6 58 159 85 0.0 6 84 1 160 49 0.0 6 49 161 73 0.0 6 72 1 162 84 0.0 6 84 163 95 0.0 6 92 3 164 88 0.0 6 88 165 62 0.0 6 62 166 64 0.0 6 64 167 74 0.0 6 74 168 45 0.0 6 45 169 37 0.0 6 37 170 49 0.0 6 49 171 56 0.0 6 56 172 47 0.0 6 45 1 1 173 58 0.0 6 58 174 44 0.0 6 44 175 62 0.0 6 62 176 55 0.0 6 54 1 177 72 0.0 6 71 1 178 58 0.0 6 56 2 179 71 0.0 6 71 180 65 0.0 6 62 3 181 59 0.0 6 56 3 182 87 0.0 6 86 1 183 53 0.0 6 53 184 86 0.0 6 83 3 185 79 0.0 6 76 3 186 120 0.0 6 118 2 187 83 0.0 6 82 1 188 84 0.0 6 83 1 189 53 0.0 6 51 2 190 91 0.0 6 91 191 106 0.0 6 104 2 192 100 0.0 6 99 1 193 122 0.0 6 121 1 194 85 0.0 6 84 1 195 91 0.0 6 90 0 0 0 1 196 126 0.0 6 125 1 197 121 0.0 6 116 5 198 112 0.0 6 104 7 0 1 199 111 0.0 6 109 2 200 153 0.0 6 149 4 201 119 0.0 6 113 6 202 116 0.0 6 116 203 218 0.0 6 214 4 204 144 0.0 6 142 2 205 211 0.0 6 207 4 206 142 0.0 6 140 2 207 204 0.0 6 200 4 208 275 0.0 6 268 7 209 191 0.0 6 188 3 210 197 0.0 6 194 2 1 211 114 0.0 6 111 3 212 114 0.0 6 110 4 213 153 0.0 6 152 1 214 217 0.0 6 215 2 215 120 0.0 6 117 3 216 203 0.0 6 198 5 217 322 0.0 6 318 4 218 174 0.0 6 172 2 219 195 0.0 6 189 6 220 177 0.0 6 171 6 221 175 0.0 6 169 6 222 224 0.0 6 223 1 223 115 0.0 6 113 1 1 224 150 0.0 6 147 2 1 225 158 0.0 6 150 7 1 226 387 0.0 6 375 12 227 323 0.0 6 308 14 1 228 369 0.0 6 360 9 229 315 0.0 6 306 9 230 265 0.0 6 259 5 1 231 226 0.0 6 218 7 1 232 316 0.0 6 308 8 233 316 0.0 6 312 4 234 291 0.0 6 283 8 235 214 0.0 6 205 9 236 158 0.0 6 155 3 237 128 0.0 6 125 3 238 170 0.0 6 165 5 239 190 0.0 6 186 3 1 240 323 0.0 6 311 12 241 300 0.0 6 293 7 242 291 0.0 6 289 2 243 256 0.0 6 248 8 244 206 0.0 6 199 7 245 314 0.0 6 306 8 246 132 0.0 6 126 6 247 217 0.0 6 210 6 1 248 126 0.0 6 121 5 249 292 0.0 6 286 6 250 232 0.0 6 221 10 0 0 0 0 1 251 272 0.0 6 262 10 252 363 0.0 6 356 6 1 253 195 0.0 6 188 7 254 378 0.0 6 369 7 2 255 279 0.0 6 271 8 256 233 0.0 6 223 10 257 269 0.0 6 259 8 1 0 0 1 258 270 0.0 6 256 13 1 259 591 0.0 6 573 18 260 570 0.0 6 549 21 261 367 0.0 6 351 13 3 262 391 0.0 6 368 22 1 263 657 0.0 6 638 17 1 1 264 356 0.0 6 337 18 1 265 459 0.0 6 450 9 266 259 0.0 6 247 11 1 267 450 0.0 6 441 8 0 0 1 268 347 0.0 6 323 24 269 410 0.0 6 403 7 270 221 0.0 6 204 15 2 271 582 0.0 6 563 19 272 809 0.0 6 793 15 1 273 422 0.0 6 411 8 3 274 469 0.0 6 455 13 1 275 667 0.0 6 655 12 276 1196 0.0 6 1160 36 277 1095 0.0 6 1070 24 1 278 675 0.0 6 659 16 279 1194 0.0 6 1171 22 1 280 1307 0.0 6 1225 77 3 2 281 22387 0.0 6 22042 338 5 0 2 282 9 0.0 6 8 0 0 1 283 2 0.0 6 2 284 1 0.0 6 1 285 1 0.0 6 1 290 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 111 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 3 0.0 6 2 1 68 1 0.0 6 1 70 3 0.0 6 3 71 2 0.0 6 2 72 5 0.0 6 5 73 2 0.0 6 2 76 1 0.0 6 1 77 2 0.0 6 2 78 2 0.0 6 2 79 6 0.0 6 6 80 3 0.0 6 3 81 1 0.0 6 1 82 2 0.0 6 2 83 1 0.0 6 1 84 2 0.0 6 2 85 1 0.0 6 1 87 2 0.0 6 2 88 3 0.0 6 3 92 1 0.0 6 1 93 2 0.0 6 2 99 1 0.0 6 1 102 1 0.0 6 1 104 1 0.0 6 1 106 1 0.0 6 1 107 2 0.0 6 2 108 1 0.0 6 1 111 1 0.0 6 1 112 1 0.0 6 1 114 1 0.0 6 1 115 1 0.0 6 1 116 1 0.0 6 1 117 1 0.0 6 1 121 2 0.0 6 2 122 3 0.0 6 3 124 1 0.0 6 1 125 1 0.0 6 1 128 1 0.0 6 1 130 1 0.0 6 1 131 1 0.0 6 1 132 1 0.0 6 1 137 1 0.0 6 1 138 3 0.0 6 3 139 1 0.0 6 1 141 1 0.0 6 1 145 3 0.0 6 3 146 1 0.0 6 1 147 2 0.0 6 2 149 1 0.0 6 1 150 1 0.0 6 1 153 1 0.0 6 1 155 4 0.0 6 4 156 2 0.0 6 2 157 1 0.0 6 1 159 1 0.0 6 1 164 1 0.0 6 1 165 2 0.0 6 2 166 2 0.0 6 2 168 1 0.0 6 1 172 1 0.0 6 1 175 2 0.0 6 2 176 1 0.0 6 1 179 1 0.0 6 1 183 1 0.0 6 1 184 1 0.0 6 1 192 1 0.0 6 1 202 1 0.0 6 1 206 1 0.0 6 1 208 1 0.0 6 1 230 1 0.0 6 1 239 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 53968 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 11 3 0.0 3 3 12 2 0.0 3 2 13 12 0.0 3 10 2 14 6 0.0 4 6 16 6 0.0 4 4 2 17 7 0.0 5 7 18 3 0.0 5 2 1 19 10 0.0 5 8 2 20 40 0.0 6 38 2 21 46 0.0 6 43 3 22 39 0.0 6 39 23 31 0.0 6 26 4 1 24 11 0.0 6 11 25 16 0.0 6 15 1 26 10 0.0 6 9 1 27 3 0.0 6 3 28 2 0.0 6 1 0 1 29 10 0.0 6 9 1 30 9 0.0 6 8 0 1 31 8 0.0 6 8 32 5 0.0 6 4 1 33 6 0.0 6 6 34 5 0.0 6 5 35 2 0.0 6 1 1 36 10 0.0 6 8 2 37 9 0.0 6 6 2 1 38 8 0.0 6 8 39 8 0.0 6 8 40 7 0.0 6 7 41 9 0.0 6 9 42 4 0.0 6 4 43 5 0.0 6 5 44 21 0.0 6 20 1 45 11 0.0 6 11 46 14 0.0 6 13 1 47 14 0.0 6 12 2 48 10 0.0 6 10 49 17 0.0 6 15 2 50 8 0.0 6 7 1 51 2 0.0 6 2 52 16 0.0 6 15 1 53 8 0.0 6 7 1 54 12 0.0 6 11 1 55 13 0.0 6 12 1 56 19 0.0 6 18 1 57 14 0.0 6 13 1 58 11 0.0 6 11 59 15 0.0 6 13 2 60 8 0.0 6 6 1 0 0 1 61 4 0.0 6 4 62 14 0.0 6 13 0 1 63 4 0.0 6 3 1 64 10 0.0 6 10 65 11 0.0 6 10 1 66 23 0.0 6 21 2 67 11 0.0 6 10 0 1 68 17 0.0 6 16 1 69 94 0.0 6 90 3 1 70 19 0.0 6 18 1 71 53 0.0 6 48 4 1 72 30 0.0 6 27 3 73 23 0.0 6 21 2 74 10 0.0 6 9 1 75 9 0.0 6 9 76 27 0.0 6 26 1 77 13 0.0 6 12 1 78 35 0.0 6 32 3 79 18 0.0 6 18 80 17 0.0 6 16 1 81 11 0.0 6 11 82 22 0.0 6 20 2 83 33 0.0 6 31 2 84 32 0.0 6 31 1 85 42 0.0 6 40 2 86 22 0.0 6 20 2 87 26 0.0 6 26 88 14 0.0 6 12 1 1 89 24 0.0 6 22 2 90 32 0.0 6 29 3 91 27 0.0 6 27 92 21 0.0 6 21 93 37 0.0 6 33 4 94 64 0.0 6 58 5 1 95 30 0.0 6 29 1 96 34 0.0 6 32 2 97 29 0.0 6 26 3 98 31 0.0 6 29 2 99 18 0.0 6 16 2 100 30 0.0 6 28 2 101 18 0.0 6 15 2 1 102 63 0.0 6 60 3 103 67 0.0 6 60 7 104 63 0.0 6 60 2 1 105 60 0.0 6 58 2 106 49 0.0 6 46 2 0 0 0 1 107 51 0.0 6 47 3 0 1 108 39 0.0 6 37 2 109 42 0.0 6 40 2 110 32 0.0 6 28 4 111 31 0.0 6 29 2 112 30 0.0 6 29 1 113 37 0.0 6 37 114 59 0.0 6 56 3 115 52 0.0 6 49 3 116 49 0.0 6 47 2 117 35 0.0 6 34 1 118 55 0.0 6 52 3 119 67 0.0 6 66 1 120 109 0.0 6 105 3 1 121 55 0.0 6 52 2 0 1 122 78 0.0 6 75 2 1 123 119 0.0 6 114 5 124 97 0.0 6 93 4 125 78 0.0 6 74 4 126 55 0.0 6 54 1 127 80 0.0 6 77 3 128 59 0.0 6 52 7 129 63 0.0 6 61 2 130 69 0.0 6 67 2 131 163 0.0 6 157 5 1 132 188 0.0 6 182 5 1 133 157 0.0 6 151 4 1 0 1 134 187 0.0 6 185 2 135 297 0.0 6 281 15 0 0 1 136 163 0.0 6 157 4 2 137 228 0.0 6 218 9 1 138 170 0.0 6 169 1 139 209 0.0 6 205 4 140 269 0.0 6 257 12 141 259 0.0 6 253 5 1 142 324 0.0 6 316 7 1 143 245 0.0 6 241 3 0 0 1 144 404 0.0 6 386 16 0 0 2 145 229 0.0 6 220 9 146 231 0.0 6 225 6 147 179 0.0 6 175 4 148 218 0.0 6 210 8 149 287 0.0 6 278 8 0 0 0 1 150 492 0.0 6 479 12 0 1 151 251 0.0 6 242 7 1 0 0 0 1 152 671 0.0 6 655 15 1 153 305 0.0 6 296 9 154 413 0.0 6 397 15 0 1 155 392 0.0 6 384 8 156 308 0.0 6 297 9 2 157 509 0.0 6 498 9 0 0 2 158 509 0.0 6 499 9 1 159 266 0.0 6 255 9 1 0 1 160 513 0.0 6 498 14 1 161 363 0.0 6 353 10 162 319 0.0 6 306 13 163 411 0.0 6 395 16 164 355 0.0 6 344 11 165 418 0.0 6 414 4 166 525 0.0 6 513 12 167 253 0.0 6 245 8 168 644 0.0 6 632 11 0 1 169 178 0.0 6 172 6 170 354 0.0 6 344 9 0 0 0 0 1 171 690 0.0 6 678 11 0 0 1 172 161 0.0 6 158 3 173 241 0.0 6 234 6 1 174 354 0.0 6 349 5 175 741 0.0 6 721 20 176 475 0.0 6 466 7 0 0 1 1 177 714 0.0 6 694 19 0 0 1 178 500 0.0 6 491 9 179 567 0.0 6 552 12 1 0 1 1 180 578 0.0 6 555 23 181 636 0.0 6 615 17 1 2 1 182 239 0.0 6 235 4 183 520 0.0 6 510 10 184 226 0.0 6 222 3 1 185 394 0.0 6 379 15 186 1216 0.0 6 1177 37 1 0 0 0 1 187 1148 0.0 6 1117 28 2 0 1 188 1021 0.0 6 997 23 0 0 1 189 1101 0.0 6 1070 31 190 1444 0.0 6 1393 50 1 191 1125 0.0 6 1091 31 0 1 2 192 885 0.0 6 858 26 0 0 1 193 650 0.0 6 633 14 3 194 855 0.0 6 831 22 1 0 1 195 1298 0.0 6 1267 29 0 0 2 196 2243 0.0 6 2199 42 2 197 363 0.0 6 347 15 1 198 1734 0.0 6 1688 45 1 199 2605 0.0 6 2558 46 1 200 667 0.0 6 642 25 201 861 0.0 6 838 20 0 1 1 0 1 202 561 0.0 6 554 7 203 550 0.0 6 533 17 204 644 0.0 6 637 7 205 301 0.0 6 296 4 0 0 1 206 261 0.0 6 256 5 207 188 0.0 6 181 7 208 357 0.0 6 348 9 209 220 0.0 6 216 4 210 235 0.0 6 226 7 0 0 1 1 211 388 0.0 6 371 16 1 212 309 0.0 6 298 10 0 0 1 213 82 0.0 6 79 3 214 316 0.0 6 308 8 215 234 0.0 6 231 3 216 695 0.0 6 677 17 0 0 1 217 428 0.0 6 411 14 2 0 0 1 218 315 0.0 6 304 10 1 219 253 0.0 6 244 9 220 210 0.0 6 206 4 221 242 0.0 6 232 10 222 453 0.0 6 441 12 223 935 0.0 6 919 15 0 0 1 224 103 0.0 6 97 6 225 237 0.0 6 232 5 226 156 0.0 6 150 5 1 227 188 0.0 6 183 5 228 143 0.0 6 140 3 229 237 0.0 6 231 4 1 0 1 230 390 0.0 6 380 10 231 135 0.0 6 134 1 232 232 0.0 6 226 6 233 223 0.0 6 219 3 0 0 1 234 139 0.0 6 134 5 235 63 0.0 6 62 1 236 60 0.0 6 59 1 237 71 0.0 6 70 1 238 42 0.0 6 38 4 239 9 0.0 6 8 1 240 60 0.0 6 60 241 58 0.0 6 57 1 242 100 0.0 6 98 2 243 115 0.0 6 112 3 244 60 0.0 6 60 245 46 0.0 6 44 2 246 28 0.0 6 28 247 18 0.0 6 17 1 248 22 0.0 6 22 249 32 0.0 6 31 1 250 30 0.0 6 28 2 251 51 0.0 6 49 2 252 21 0.0 6 21 253 22 0.0 6 22 254 1 0.0 6 1 255 3 0.0 6 3 256 2 0.0 6 2 257 1 0.0 6 1 276 1 0.0 6 1 277 1 0.0 6 1 278 2 0.0 6 2 279 9 0.0 6 9 280 40 0.0 6 40 281 185 0.0 6 179 6 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 134 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 50 5 0.0 6 5 69 3 0.0 6 3 71 1 0.0 6 1 72 4 0.0 6 4 73 1 0.0 6 1 74 1 0.0 6 1 75 1 0.0 6 1 76 2 0.0 6 2 77 1 0.0 6 1 78 2 0.0 6 2 79 3 0.0 6 3 80 1 0.0 6 1 83 1 0.0 6 1 85 2 0.0 6 2 86 1 0.0 6 1 89 3 0.0 6 3 90 1 0.0 6 1 91 2 0.0 6 2 92 21 0.0 6 21 93 3 0.0 6 3 94 1 0.0 6 1 95 1 0.0 6 1 97 1 0.0 6 1 99 6 0.0 6 6 100 1 0.0 6 1 101 1 0.0 6 1 102 1 0.0 6 1 104 1 0.0 6 1 105 3 0.0 6 3 107 4 0.0 6 4 108 1 0.0 6 1 110 1 0.0 6 1 111 2 0.0 6 2 112 1 0.0 6 1 114 4 0.0 6 3 0 0 1 115 1 0.0 6 1 116 2 0.0 6 2 117 1 0.0 6 1 118 1 0.0 6 1 119 1 0.0 6 1 121 1 0.0 6 1 122 1 0.0 6 1 125 1 0.0 6 1 126 1 0.0 6 1 131 1 0.0 6 1 132 1 0.0 6 1 135 1 0.0 6 1 136 1 0.0 6 1 137 2 0.0 6 2 138 2 0.0 6 2 146 1 0.0 6 1 147 2 0.0 6 2 150 1 0.0 6 1 152 2 0.0 6 2 153 1 0.0 6 1 154 2 0.0 6 2 156 1 0.0 6 1 157 1 0.0 6 1 159 2 0.0 6 2 160 1 0.0 6 1 163 2 0.0 6 2 166 1 0.0 6 1 171 1 0.0 6 1 173 1 0.0 6 1 175 1 0.0 6 1 176 2 0.0 6 2 177 2 0.0 6 2 178 1 0.0 6 0 0 1 180 2 0.0 6 2 194 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_20_71_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_20_298_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_20_71_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_20_298_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.70 s (36 µs/read; 1.69 M reads/minute). === Summary === Total read pairs processed: 47,683 Read 1 with adapter: 47,678 (100.0%) Read 2 with adapter: 47,644 (99.9%) == Read fate breakdown == Pairs that were too short: 11 (0.0%) Pairs discarded as untrimmed: 43 (0.1%) Pairs written (passing filters): 47,629 (99.9%) Total basepairs processed: 22,603,414 bp Read 1: 12,720,257 bp Read 2: 9,883,157 bp Total written (filtered): 2,993,262 bp (13.2%) Read 1: 1,454,903 bp Read 2: 1,538,359 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 47192 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 2 186.3 1 1 1 6 1 11.6 1 1 9 3 0.2 2 3 11 1 0.0 3 0 1 15 1 0.0 4 1 17 1 0.0 5 1 19 1 0.0 5 0 1 20 44 0.0 6 44 21 27 0.0 6 27 22 25 0.0 6 25 23 16 0.0 6 16 24 11 0.0 6 11 25 15 0.0 6 14 1 26 7 0.0 6 7 27 3 0.0 6 3 28 4 0.0 6 4 29 5 0.0 6 5 30 3 0.0 6 3 31 3 0.0 6 2 1 32 4 0.0 6 4 33 4 0.0 6 4 34 6 0.0 6 6 35 3 0.0 6 3 36 2 0.0 6 1 1 37 2 0.0 6 2 38 3 0.0 6 3 39 4 0.0 6 4 40 2 0.0 6 2 41 4 0.0 6 2 1 0 0 0 0 1 42 3 0.0 6 3 43 1 0.0 6 1 44 3 0.0 6 3 45 4 0.0 6 4 46 2 0.0 6 2 47 4 0.0 6 3 1 48 2 0.0 6 2 49 1 0.0 6 1 50 1 0.0 6 1 51 4 0.0 6 4 53 2 0.0 6 0 1 1 54 1 0.0 6 1 55 2 0.0 6 2 56 2 0.0 6 1 0 0 1 57 1 0.0 6 1 58 2 0.0 6 1 1 59 2 0.0 6 2 60 5 0.0 6 4 1 62 3 0.0 6 3 63 5 0.0 6 5 64 3 0.0 6 1 2 65 4 0.0 6 4 66 3 0.0 6 3 67 4 0.0 6 4 68 3 0.0 6 3 69 1 0.0 6 1 71 2 0.0 6 2 73 3 0.0 6 2 1 74 3 0.0 6 3 75 3 0.0 6 3 76 4 0.0 6 3 1 77 4 0.0 6 3 1 78 5 0.0 6 4 1 79 5 0.0 6 5 80 6 0.0 6 6 81 4 0.0 6 4 82 2 0.0 6 2 84 2 0.0 6 2 85 3 0.0 6 2 0 0 0 0 1 86 7 0.0 6 7 87 4 0.0 6 4 88 2 0.0 6 2 89 5 0.0 6 5 90 2 0.0 6 2 91 3 0.0 6 3 92 4 0.0 6 4 93 3 0.0 6 3 94 6 0.0 6 6 95 2 0.0 6 2 96 4 0.0 6 4 97 4 0.0 6 4 98 6 0.0 6 6 99 5 0.0 6 3 1 0 0 0 1 100 8 0.0 6 8 101 8 0.0 6 8 102 4 0.0 6 4 103 4 0.0 6 4 105 9 0.0 6 9 106 3 0.0 6 2 1 107 8 0.0 6 8 108 6 0.0 6 6 109 9 0.0 6 9 110 10 0.0 6 10 111 2 0.0 6 2 112 7 0.0 6 5 1 1 113 7 0.0 6 7 114 2 0.0 6 2 115 9 0.0 6 9 116 5 0.0 6 5 117 13 0.0 6 13 118 8 0.0 6 8 119 6 0.0 6 6 120 8 0.0 6 8 121 10 0.0 6 10 122 9 0.0 6 9 123 8 0.0 6 8 124 9 0.0 6 9 125 5 0.0 6 5 126 9 0.0 6 8 1 127 11 0.0 6 11 128 10 0.0 6 9 1 129 14 0.0 6 13 0 0 0 0 1 130 14 0.0 6 14 131 8 0.0 6 8 132 17 0.0 6 17 133 17 0.0 6 17 134 24 0.0 6 24 135 13 0.0 6 12 0 1 136 13 0.0 6 13 137 15 0.0 6 15 138 13 0.0 6 13 139 11 0.0 6 11 140 20 0.0 6 20 141 31 0.0 6 31 142 33 0.0 6 31 2 143 205 0.0 6 205 144 106 0.0 6 106 145 133 0.0 6 132 1 146 117 0.0 6 117 147 222 0.0 6 220 2 148 79 0.0 6 78 0 0 1 149 109 0.0 6 109 150 102 0.0 6 102 151 211 0.0 6 211 152 58 0.0 6 58 153 99 0.0 6 99 154 57 0.0 6 57 155 108 0.0 6 108 156 44 0.0 6 43 1 157 89 0.0 6 88 1 158 75 0.0 6 75 159 95 0.0 6 95 160 118 0.0 6 118 161 98 0.0 6 98 162 54 0.0 6 53 1 163 34 0.0 6 33 0 1 164 69 0.0 6 69 165 143 0.0 6 142 1 166 102 0.0 6 102 167 359 0.0 6 359 168 76 0.0 6 74 2 169 115 0.0 6 114 1 170 115 0.0 6 115 171 225 0.0 6 225 172 85 0.0 6 84 1 173 146 0.0 6 146 174 281 0.0 6 280 1 175 119 0.0 6 119 176 164 0.0 6 164 177 385 0.0 6 384 1 178 105 0.0 6 103 2 179 121 0.0 6 120 1 180 208 0.0 6 206 2 181 313 0.0 6 313 182 108 0.0 6 108 183 60 0.0 6 59 1 184 99 0.0 6 95 4 185 160 0.0 6 156 4 186 102 0.0 6 100 2 187 77 0.0 6 73 4 188 121 0.0 6 118 3 189 123 0.0 6 122 1 190 226 0.0 6 224 2 191 238 0.0 6 237 1 192 157 0.0 6 156 1 193 343 0.0 6 340 3 194 291 0.0 6 291 195 143 0.0 6 142 1 196 136 0.0 6 134 2 197 210 0.0 6 206 4 198 188 0.0 6 188 199 330 0.0 6 324 4 1 1 200 251 0.0 6 246 4 0 0 0 1 201 204 0.0 6 203 1 202 196 0.0 6 195 0 0 0 0 0 1 203 205 0.0 6 204 1 204 176 0.0 6 174 2 205 426 0.0 6 422 4 206 236 0.0 6 233 3 207 304 0.0 6 300 4 208 476 0.0 6 474 2 209 282 0.0 6 278 4 210 388 0.0 6 380 7 0 1 211 298 0.0 6 294 3 1 212 257 0.0 6 255 2 213 274 0.0 6 267 7 214 313 0.0 6 310 2 0 1 215 448 0.0 6 443 5 216 272 0.0 6 269 2 0 1 217 395 0.0 6 392 3 218 273 0.0 6 270 3 219 245 0.0 6 241 3 0 0 1 220 292 0.0 6 288 3 1 221 323 0.0 6 319 4 222 261 0.0 6 255 6 223 294 0.0 6 291 3 224 282 0.0 6 280 2 225 268 0.0 6 260 7 0 1 226 294 0.0 6 288 6 227 359 0.0 6 352 7 228 422 0.0 6 418 4 229 336 0.0 6 332 4 230 290 0.0 6 288 2 231 272 0.0 6 264 7 1 232 364 0.0 6 360 4 233 249 0.0 6 247 2 234 262 0.0 6 258 4 235 305 0.0 6 301 4 236 331 0.0 6 326 5 237 375 0.0 6 370 4 1 238 413 0.0 6 409 4 239 264 0.0 6 260 4 240 242 0.0 6 238 4 241 266 0.0 6 260 6 242 234 0.0 6 230 4 243 246 0.0 6 240 6 244 325 0.0 6 319 6 245 258 0.0 6 253 5 246 160 0.0 6 156 4 247 294 0.0 6 286 8 248 249 0.0 6 242 7 249 370 0.0 6 362 8 250 271 0.0 6 266 5 251 245 0.0 6 241 4 252 303 0.0 6 292 10 1 253 299 0.0 6 286 12 0 1 254 441 0.0 6 432 7 2 255 248 0.0 6 235 13 256 550 0.0 6 544 6 257 248 0.0 6 242 6 258 341 0.0 6 330 8 3 259 336 0.0 6 329 7 260 628 0.0 6 614 13 1 261 260 0.0 6 251 9 262 257 0.0 6 249 8 263 297 0.0 6 286 11 264 354 0.0 6 346 8 265 290 0.0 6 287 3 266 329 0.0 6 323 6 267 260 0.0 6 252 8 268 422 0.0 6 411 11 269 226 0.0 6 217 8 0 1 270 297 0.0 6 288 8 1 271 317 0.0 6 308 9 272 381 0.0 6 365 15 1 273 416 0.0 6 406 9 0 1 274 420 0.0 6 406 12 1 1 275 563 0.0 6 549 14 276 669 0.0 6 654 12 3 277 655 0.0 6 641 14 278 656 0.0 6 631 22 2 1 279 799 0.0 6 775 23 1 280 2991 0.0 6 2900 87 2 2 281 8277 0.0 6 8093 179 5 282 37 0.0 6 31 6 283 1 0.0 6 0 1 284 2 0.0 6 1 0 0 0 1 285 1 0.0 6 0 0 0 1 286 1 0.0 6 1 291 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 486 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 68 1 0.0 6 1 69 3 0.0 6 3 70 5 0.0 6 5 71 4 0.0 6 4 72 2 0.0 6 2 73 5 0.0 6 5 74 7 0.0 6 7 75 1 0.0 6 1 76 2 0.0 6 2 77 2 0.0 6 2 78 5 0.0 6 4 1 79 2 0.0 6 2 80 4 0.0 6 4 82 1 0.0 6 1 85 1 0.0 6 1 86 3 0.0 6 3 87 3 0.0 6 3 88 3 0.0 6 3 90 3 0.0 6 3 91 1 0.0 6 1 92 4 0.0 6 4 93 4 0.0 6 4 94 8 0.0 6 8 96 3 0.0 6 3 98 5 0.0 6 5 99 5 0.0 6 5 100 4 0.0 6 4 101 7 0.0 6 7 102 2 0.0 6 2 103 6 0.0 6 6 104 12 0.0 6 12 105 1 0.0 6 1 106 2 0.0 6 2 107 6 0.0 6 6 108 10 0.0 6 10 109 1 0.0 6 1 110 1 0.0 6 1 111 1 0.0 6 1 112 5 0.0 6 5 115 1 0.0 6 1 116 6 0.0 6 6 117 4 0.0 6 4 119 2 0.0 6 2 120 11 0.0 6 11 121 4 0.0 6 4 123 1 0.0 6 1 125 3 0.0 6 3 126 4 0.0 6 4 127 1 0.0 6 1 128 2 0.0 6 2 129 1 0.0 6 1 131 5 0.0 6 5 132 6 0.0 6 6 134 5 0.0 6 5 135 3 0.0 6 3 136 3 0.0 6 3 137 5 0.0 6 5 138 8 0.0 6 8 139 7 0.0 6 7 140 1 0.0 6 1 141 5 0.0 6 5 142 3 0.0 6 3 144 3 0.0 6 3 145 9 0.0 6 9 146 1 0.0 6 1 147 3 0.0 6 3 148 6 0.0 6 6 149 2 0.0 6 2 150 4 0.0 6 4 151 4 0.0 6 4 153 3 0.0 6 3 154 3 0.0 6 3 155 2 0.0 6 2 156 4 0.0 6 4 159 1 0.0 6 1 160 1 0.0 6 1 161 7 0.0 6 7 162 5 0.0 6 5 163 7 0.0 6 7 165 6 0.0 6 6 166 1 0.0 6 1 167 3 0.0 6 3 168 7 0.0 6 7 169 3 0.0 6 3 170 1 0.0 6 1 171 4 0.0 6 4 173 3 0.0 6 3 174 7 0.0 6 7 175 12 0.0 6 12 176 1 0.0 6 1 177 3 0.0 6 3 179 3 0.0 6 3 181 2 0.0 6 2 182 2 0.0 6 2 183 8 0.0 6 8 184 2 0.0 6 2 187 7 0.0 6 7 188 6 0.0 6 6 189 1 0.0 6 1 192 5 0.0 6 5 194 3 0.0 6 2 1 195 7 0.0 6 7 196 3 0.0 6 3 197 6 0.0 6 6 198 15 0.0 6 15 199 9 0.0 6 9 200 2 0.0 6 2 201 1 0.0 6 1 207 1 0.0 6 1 208 2 0.0 6 2 214 2 0.0 6 2 218 1 0.0 6 1 219 3 0.0 6 3 220 7 0.0 6 7 221 1 0.0 6 1 222 4 0.0 6 4 223 3 0.0 6 3 224 1 0.0 6 1 228 3 0.0 6 3 229 1 0.0 6 1 236 1 0.0 6 1 237 1 0.0 6 1 245 1 0.0 6 1 246 2 0.0 6 2 252 2 0.0 6 2 254 5 0.0 6 5 267 1 0.0 6 1 270 1 0.0 6 1 277 1 0.0 6 1 278 2 0.0 6 2 279 1 0.0 6 1 281 1 0.0 6 1 294 2 0.0 6 2 295 1 0.0 6 1 303 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 47154 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 745.0 0 1 1 4 1 186.3 1 0 1 6 1 11.6 1 1 10 5 0.0 3 4 1 11 3 0.0 3 3 12 1 0.0 3 1 13 3 0.0 3 3 14 2 0.0 4 2 16 7 0.0 4 6 1 17 11 0.0 5 11 18 7 0.0 5 7 19 9 0.0 5 7 1 1 20 61 0.0 6 58 3 21 38 0.0 6 35 3 22 29 0.0 6 27 2 23 22 0.0 6 21 1 24 17 0.0 6 17 25 17 0.0 6 17 26 16 0.0 6 15 1 27 10 0.0 6 10 28 14 0.0 6 14 29 11 0.0 6 11 30 11 0.0 6 9 2 31 5 0.0 6 4 1 32 3 0.0 6 3 33 5 0.0 6 5 34 5 0.0 6 5 35 3 0.0 6 3 36 3 0.0 6 3 37 4 0.0 6 4 38 4 0.0 6 4 39 7 0.0 6 5 2 40 7 0.0 6 6 1 41 12 0.0 6 10 2 42 5 0.0 6 2 3 43 1 0.0 6 1 44 13 0.0 6 13 45 7 0.0 6 4 3 46 12 0.0 6 9 3 47 11 0.0 6 11 48 8 0.0 6 7 1 49 15 0.0 6 14 1 50 9 0.0 6 7 2 51 17 0.0 6 14 3 52 15 0.0 6 13 1 1 53 8 0.0 6 8 54 7 0.0 6 7 55 8 0.0 6 8 56 12 0.0 6 12 57 21 0.0 6 16 3 0 1 0 1 58 14 0.0 6 12 2 59 2 0.0 6 2 60 11 0.0 6 10 1 61 11 0.0 6 10 1 62 12 0.0 6 10 1 1 63 17 0.0 6 16 1 64 14 0.0 6 13 1 65 7 0.0 6 7 66 22 0.0 6 20 2 67 88 0.0 6 83 4 1 68 29 0.0 6 27 2 69 38 0.0 6 33 5 70 13 0.0 6 11 2 71 39 0.0 6 36 3 72 20 0.0 6 20 73 14 0.0 6 13 1 74 12 0.0 6 12 75 23 0.0 6 22 1 76 26 0.0 6 24 1 1 77 18 0.0 6 18 78 12 0.0 6 8 4 79 23 0.0 6 22 1 80 16 0.0 6 14 1 0 0 0 0 1 81 15 0.0 6 15 82 17 0.0 6 15 1 1 83 36 0.0 6 33 2 0 0 0 1 84 28 0.0 6 28 85 25 0.0 6 21 4 86 26 0.0 6 25 1 87 26 0.0 6 25 0 1 88 17 0.0 6 17 89 28 0.0 6 28 90 28 0.0 6 26 2 91 20 0.0 6 18 2 92 33 0.0 6 31 2 93 28 0.0 6 27 1 94 55 0.0 6 51 4 95 35 0.0 6 31 2 2 96 36 0.0 6 34 2 97 32 0.0 6 31 1 98 29 0.0 6 29 99 34 0.0 6 30 2 2 100 28 0.0 6 28 101 41 0.0 6 37 3 1 102 37 0.0 6 35 2 103 51 0.0 6 51 104 67 0.0 6 64 3 105 42 0.0 6 39 3 106 41 0.0 6 37 4 107 39 0.0 6 38 1 108 37 0.0 6 35 2 109 46 0.0 6 45 1 110 46 0.0 6 42 3 1 111 58 0.0 6 57 1 112 65 0.0 6 64 1 113 54 0.0 6 51 3 114 64 0.0 6 62 1 1 115 54 0.0 6 53 1 116 44 0.0 6 44 117 62 0.0 6 60 2 118 67 0.0 6 64 3 119 66 0.0 6 65 1 120 83 0.0 6 78 5 121 50 0.0 6 47 3 122 82 0.0 6 80 1 0 0 0 1 123 82 0.0 6 79 3 124 99 0.0 6 99 125 93 0.0 6 90 3 126 89 0.0 6 87 2 127 110 0.0 6 107 3 128 72 0.0 6 68 3 0 0 0 1 129 86 0.0 6 82 4 130 85 0.0 6 84 1 131 129 0.0 6 118 10 0 1 132 165 0.0 6 160 5 133 154 0.0 6 150 4 134 207 0.0 6 199 8 135 269 0.0 6 265 4 136 173 0.0 6 171 2 137 208 0.0 6 204 4 138 262 0.0 6 259 2 1 139 257 0.0 6 253 4 140 350 0.0 6 343 5 1 0 1 141 328 0.0 6 319 8 0 0 1 142 392 0.0 6 386 6 143 289 0.0 6 282 7 144 398 0.0 6 392 6 145 325 0.0 6 321 2 0 1 1 146 420 0.0 6 414 4 1 1 147 250 0.0 6 243 5 0 1 1 148 359 0.0 6 351 7 0 0 0 1 149 433 0.0 6 426 6 0 1 150 681 0.0 6 664 17 151 380 0.0 6 371 9 152 585 0.0 6 576 7 1 0 1 153 475 0.0 6 466 8 0 0 1 154 471 0.0 6 463 7 1 155 471 0.0 6 467 4 156 431 0.0 6 426 4 0 0 1 157 528 0.0 6 512 13 1 2 158 523 0.0 6 511 9 0 2 1 159 369 0.0 6 364 4 1 160 495 0.0 6 488 5 1 0 0 1 161 369 0.0 6 358 10 1 162 394 0.0 6 389 4 0 0 1 163 405 0.0 6 401 2 1 0 0 1 164 416 0.0 6 403 12 0 0 0 1 165 409 0.0 6 400 9 166 564 0.0 6 556 8 167 323 0.0 6 315 6 0 0 0 1 1 168 636 0.0 6 622 11 1 0 2 169 435 0.0 6 431 3 0 0 1 170 436 0.0 6 424 10 1 0 1 171 679 0.0 6 664 13 1 0 1 172 488 0.0 6 479 8 0 0 0 1 173 419 0.0 6 410 8 0 0 1 174 484 0.0 6 473 10 1 175 704 0.0 6 688 13 1 1 1 176 544 0.0 6 537 7 177 529 0.0 6 515 14 178 509 0.0 6 500 9 179 490 0.0 6 474 15 0 0 1 180 504 0.0 6 489 13 2 181 394 0.0 6 382 12 182 245 0.0 6 240 5 183 550 0.0 6 538 11 1 184 658 0.0 6 644 13 0 0 1 185 834 0.0 6 807 27 186 790 0.0 6 772 18 187 772 0.0 6 749 21 2 188 770 0.0 6 746 19 3 1 1 189 571 0.0 6 553 17 1 190 776 0.0 6 748 26 1 0 1 191 778 0.0 6 750 27 1 192 477 0.0 6 460 17 193 593 0.0 6 577 13 2 0 1 194 464 0.0 6 453 10 1 195 772 0.0 6 746 26 196 999 0.0 6 981 18 197 578 0.0 6 562 15 1 198 821 0.0 6 799 21 1 199 1066 0.0 6 1035 30 0 0 1 200 452 0.0 6 439 13 201 612 0.0 6 594 17 1 202 423 0.0 6 411 12 203 331 0.0 6 322 9 204 411 0.0 6 398 13 205 385 0.0 6 374 11 206 202 0.0 6 196 5 0 0 1 207 276 0.0 6 268 8 208 318 0.0 6 310 7 0 0 1 209 253 0.0 6 251 2 210 192 0.0 6 187 5 211 295 0.0 6 286 8 0 0 1 212 343 0.0 6 330 11 2 213 253 0.0 6 246 5 1 0 0 1 214 367 0.0 6 355 12 215 237 0.0 6 232 5 216 326 0.0 6 310 15 1 217 148 0.0 6 142 6 218 177 0.0 6 169 8 219 165 0.0 6 157 7 0 1 220 77 0.0 6 73 4 221 220 0.0 6 211 7 2 222 236 0.0 6 227 8 0 0 1 223 298 0.0 6 289 9 224 271 0.0 6 264 6 0 1 225 234 0.0 6 227 7 226 207 0.0 6 197 9 1 227 354 0.0 6 349 5 228 170 0.0 6 162 7 0 0 1 229 373 0.0 6 364 9 230 90 0.0 6 87 2 0 1 231 177 0.0 6 170 6 0 1 232 167 0.0 6 163 4 233 95 0.0 6 94 1 234 110 0.0 6 108 2 235 68 0.0 6 68 236 9 0.0 6 8 0 0 1 237 23 0.0 6 21 2 238 48 0.0 6 48 239 28 0.0 6 28 240 37 0.0 6 36 1 241 68 0.0 6 66 2 242 111 0.0 6 110 1 243 81 0.0 6 80 1 244 61 0.0 6 60 1 245 41 0.0 6 40 1 246 46 0.0 6 44 2 247 32 0.0 6 32 248 92 0.0 6 91 1 249 33 0.0 6 33 250 16 0.0 6 15 1 251 75 0.0 6 68 6 0 1 252 19 0.0 6 19 253 8 0.0 6 8 254 1 0.0 6 1 255 1 0.0 6 1 266 1 0.0 6 1 268 1 0.0 6 1 277 2 0.0 6 2 278 21 0.0 6 21 279 69 0.0 6 69 280 1 0.0 6 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 490 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 68 1 0.0 6 1 71 1 0.0 6 1 72 7 0.0 6 7 73 5 0.0 6 5 74 5 0.0 6 5 75 4 0.0 6 4 76 5 0.0 6 5 78 2 0.0 6 2 79 6 0.0 6 6 80 7 0.0 6 7 81 2 0.0 6 2 82 5 0.0 6 5 83 3 0.0 6 3 84 4 0.0 6 4 85 1 0.0 6 1 86 4 0.0 6 4 87 1 0.0 6 1 88 2 0.0 6 2 89 5 0.0 6 5 90 4 0.0 6 4 93 3 0.0 6 3 94 5 0.0 6 5 95 9 0.0 6 9 96 11 0.0 6 11 98 5 0.0 6 4 0 0 0 1 99 6 0.0 6 6 100 5 0.0 6 5 101 2 0.0 6 2 102 7 0.0 6 7 103 15 0.0 6 15 105 3 0.0 6 3 106 9 0.0 6 9 107 4 0.0 6 4 108 5 0.0 6 5 109 6 0.0 6 6 110 7 0.0 6 7 111 5 0.0 6 5 113 2 0.0 6 2 114 6 0.0 6 6 117 2 0.0 6 2 118 3 0.0 6 2 0 0 0 1 119 6 0.0 6 6 120 3 0.0 6 3 121 4 0.0 6 4 122 11 0.0 6 11 123 3 0.0 6 3 126 2 0.0 6 2 127 4 0.0 6 4 128 6 0.0 6 6 129 3 0.0 6 3 131 3 0.0 6 3 133 3 0.0 6 3 134 6 0.0 6 6 135 1 0.0 6 1 136 1 0.0 6 1 137 3 0.0 6 3 138 3 0.0 6 3 139 10 0.0 6 10 140 10 0.0 6 10 141 9 0.0 6 9 142 1 0.0 6 1 143 3 0.0 6 3 144 13 0.0 6 13 145 1 0.0 6 1 146 1 0.0 6 1 147 8 0.0 6 8 148 1 0.0 6 0 0 0 0 1 149 1 0.0 6 1 150 8 0.0 6 8 151 6 0.0 6 6 152 2 0.0 6 2 153 12 0.0 6 12 155 1 0.0 6 1 156 3 0.0 6 3 157 2 0.0 6 2 158 6 0.0 6 6 159 1 0.0 6 1 160 2 0.0 6 1 0 0 0 1 163 10 0.0 6 10 164 4 0.0 6 4 165 7 0.0 6 7 166 2 0.0 6 2 167 5 0.0 6 5 168 3 0.0 6 3 169 3 0.0 6 3 170 8 0.0 6 8 171 3 0.0 6 3 173 1 0.0 6 1 174 4 0.0 6 4 175 4 0.0 6 4 176 7 0.0 6 7 177 11 0.0 6 11 178 1 0.0 6 1 179 8 0.0 6 8 180 1 0.0 6 1 181 2 0.0 6 2 183 1 0.0 6 1 184 3 0.0 6 3 185 5 0.0 6 5 186 3 0.0 6 3 190 3 0.0 6 3 191 2 0.0 6 2 194 2 0.0 6 2 196 3 0.0 6 3 197 1 0.0 6 1 198 2 0.0 6 2 199 5 0.0 6 5 200 9 0.0 6 9 201 3 0.0 6 3 203 1 0.0 6 1 207 2 0.0 6 2 210 2 0.0 6 2 222 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_01_60_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_01_287_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_01_60_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_01_287_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_02_61_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_02_288_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_02_61_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_02_288_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_03_62_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_03_289_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_03_62_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_03_289_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_04_63_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_04_290_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_04_63_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_04_290_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_05_64_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_05_291_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_05_64_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_05_291_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_06_65_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_06_292_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_06_65_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_06_292_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_15_66_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_15_293_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_15_66_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_15_293_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_16_67_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_16_294_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_16_67_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_16_294_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_17_68_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_17_295_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_17_68_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_17_295_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_18_69_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_18_296_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_18_69_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_18_296_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_19_70_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_19_297_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_19_70_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_19_297_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_20_71_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_06-Rev_20_298_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_20_71_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_06-Rev_20_298_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_01_72_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_01_299_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_01_72_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_01_299_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.53 s (25 µs/read; 2.36 M reads/minute). === Summary === Total read pairs processed: 60,143 Read 1 with adapter: 60,141 (100.0%) Read 2 with adapter: 60,095 (99.9%) == Read fate breakdown == Pairs that were too short: 31 (0.1%) Pairs discarded as untrimmed: 50 (0.1%) Pairs written (passing filters): 60,062 (99.9%) Total basepairs processed: 26,944,887 bp Read 1: 15,470,850 bp Read 2: 11,474,037 bp Total written (filtered): 3,834,760 bp (14.2%) Read 1: 2,016,124 bp Read 2: 1,818,636 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 59147 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 939.7 0 2 4 2 234.9 1 1 1 6 1 14.7 1 0 1 7 2 3.7 2 2 8 1 0.9 2 1 16 2 0.0 4 2 17 1 0.0 5 1 18 1 0.0 5 1 19 3 0.0 5 2 1 20 68 0.0 6 67 1 21 33 0.0 6 29 4 22 21 0.0 6 21 23 10 0.0 6 10 24 17 0.0 6 17 25 11 0.0 6 11 26 12 0.0 6 12 27 7 0.0 6 7 28 8 0.0 6 8 29 6 0.0 6 6 30 2 0.0 6 2 31 4 0.0 6 4 32 3 0.0 6 3 33 8 0.0 6 7 0 0 1 34 5 0.0 6 5 35 2 0.0 6 1 0 0 0 1 36 6 0.0 6 5 1 37 3 0.0 6 1 1 0 0 0 1 38 6 0.0 6 6 39 3 0.0 6 3 40 2 0.0 6 1 1 41 10 0.0 6 10 42 2 0.0 6 2 43 4 0.0 6 4 44 1 0.0 6 1 45 9 0.0 6 9 46 10 0.0 6 10 47 5 0.0 6 5 48 4 0.0 6 4 49 2 0.0 6 2 50 2 0.0 6 2 51 6 0.0 6 5 1 52 7 0.0 6 5 1 1 53 5 0.0 6 4 0 0 0 0 1 54 2 0.0 6 2 55 4 0.0 6 3 0 0 1 56 2 0.0 6 2 57 8 0.0 6 8 58 4 0.0 6 3 1 59 4 0.0 6 4 60 5 0.0 6 4 1 61 1 0.0 6 1 62 7 0.0 6 6 1 63 5 0.0 6 5 64 1 0.0 6 1 65 8 0.0 6 7 0 0 0 0 1 66 8 0.0 6 8 67 3 0.0 6 3 68 4 0.0 6 4 69 6 0.0 6 5 1 70 6 0.0 6 5 1 71 4 0.0 6 4 72 5 0.0 6 5 73 6 0.0 6 6 74 4 0.0 6 4 75 2 0.0 6 2 76 2 0.0 6 2 77 5 0.0 6 5 78 8 0.0 6 8 79 5 0.0 6 5 80 5 0.0 6 4 1 81 6 0.0 6 6 82 15 0.0 6 13 1 0 1 83 10 0.0 6 9 1 84 12 0.0 6 12 85 2 0.0 6 1 1 86 13 0.0 6 12 0 1 87 8 0.0 6 6 2 88 6 0.0 6 6 89 10 0.0 6 10 90 3 0.0 6 3 91 12 0.0 6 11 1 92 3 0.0 6 3 93 10 0.0 6 7 2 0 0 0 1 94 11 0.0 6 11 95 9 0.0 6 9 96 2 0.0 6 2 97 10 0.0 6 9 1 98 11 0.0 6 11 99 7 0.0 6 7 100 6 0.0 6 6 101 10 0.0 6 8 2 102 12 0.0 6 11 1 103 15 0.0 6 14 1 104 16 0.0 6 15 1 105 9 0.0 6 8 1 106 10 0.0 6 10 107 10 0.0 6 9 1 108 14 0.0 6 14 109 12 0.0 6 12 110 14 0.0 6 14 111 15 0.0 6 14 1 112 14 0.0 6 14 113 20 0.0 6 20 114 15 0.0 6 15 115 17 0.0 6 15 2 116 16 0.0 6 16 117 13 0.0 6 12 1 118 13 0.0 6 13 119 11 0.0 6 11 120 16 0.0 6 14 1 0 0 1 121 14 0.0 6 14 122 18 0.0 6 18 123 21 0.0 6 20 1 124 21 0.0 6 21 125 27 0.0 6 26 0 1 126 28 0.0 6 28 127 23 0.0 6 23 128 26 0.0 6 25 1 129 24 0.0 6 22 2 130 20 0.0 6 20 131 31 0.0 6 31 132 22 0.0 6 22 133 20 0.0 6 20 134 36 0.0 6 34 1 0 0 0 0 1 135 33 0.0 6 33 136 27 0.0 6 27 137 27 0.0 6 27 138 29 0.0 6 29 139 39 0.0 6 38 1 140 38 0.0 6 38 141 91 0.0 6 90 0 1 142 203 0.0 6 203 143 250 0.0 6 246 4 144 150 0.0 6 149 1 145 204 0.0 6 201 3 146 196 0.0 6 195 0 1 147 231 0.0 6 230 1 148 142 0.0 6 141 0 0 1 149 156 0.0 6 153 3 150 112 0.0 6 111 1 151 115 0.0 6 115 152 96 0.0 6 94 2 153 124 0.0 6 123 0 0 1 154 81 0.0 6 81 155 127 0.0 6 124 3 156 87 0.0 6 86 1 157 123 0.0 6 119 3 0 1 158 130 0.0 6 127 3 159 140 0.0 6 138 2 160 124 0.0 6 122 1 0 1 161 178 0.0 6 176 2 162 171 0.0 6 167 4 163 159 0.0 6 157 1 0 1 164 203 0.0 6 198 5 165 104 0.0 6 102 2 166 181 0.0 6 179 2 167 154 0.0 6 150 2 1 0 1 168 165 0.0 6 164 1 169 167 0.0 6 167 170 132 0.0 6 132 171 148 0.0 6 142 3 1 0 0 1 1 172 170 0.0 6 167 3 173 178 0.0 6 172 3 1 2 174 180 0.0 6 177 3 175 191 0.0 6 188 3 176 192 0.0 6 188 4 177 186 0.0 6 186 178 193 0.0 6 188 3 1 0 0 1 179 466 0.0 6 460 3 2 0 0 1 180 201 0.0 6 197 4 181 173 0.0 6 169 2 0 0 1 0 1 182 228 0.0 6 224 3 1 183 313 0.0 6 308 3 0 0 0 1 1 184 234 0.0 6 232 2 185 217 0.0 6 214 3 186 246 0.0 6 244 2 187 210 0.0 6 206 4 188 226 0.0 6 223 3 189 230 0.0 6 228 2 190 286 0.0 6 278 5 0 1 1 0 1 191 332 0.0 6 327 4 0 0 0 1 192 385 0.0 6 381 3 0 0 1 193 391 0.0 6 388 3 194 409 0.0 6 405 4 195 554 0.0 6 546 8 196 565 0.0 6 551 13 0 0 0 0 1 197 647 0.0 6 635 11 1 198 507 0.0 6 496 11 199 398 0.0 6 388 9 0 0 0 0 1 200 475 0.0 6 467 8 201 427 0.0 6 416 8 1 1 0 0 1 202 491 0.0 6 484 5 1 1 203 515 0.0 6 503 10 0 0 0 1 1 204 525 0.0 6 514 10 1 205 608 0.0 6 592 13 1 1 0 0 1 206 523 0.0 6 515 5 0 0 0 0 3 207 528 0.0 6 515 13 208 460 0.0 6 448 12 209 500 0.0 6 490 10 210 671 0.0 6 662 9 211 627 0.0 6 613 10 2 1 0 0 1 212 643 0.0 6 630 11 1 0 0 1 213 715 0.0 6 703 11 0 0 0 1 214 835 0.0 6 818 16 0 0 1 215 649 0.0 6 634 14 0 0 0 1 216 660 0.0 6 644 14 0 0 0 0 2 217 657 0.0 6 653 4 218 682 0.0 6 672 9 1 219 501 0.0 6 493 6 1 0 1 220 471 0.0 6 465 6 221 532 0.0 6 521 8 2 0 0 0 1 222 536 0.0 6 532 4 223 527 0.0 6 520 6 0 1 224 640 0.0 6 632 6 1 0 1 225 655 0.0 6 648 6 1 226 577 0.0 6 569 6 2 227 489 0.0 6 479 8 1 1 228 496 0.0 6 490 5 1 229 520 0.0 6 512 6 0 0 0 0 2 230 478 0.0 6 469 8 0 0 0 1 231 524 0.0 6 512 9 1 0 1 1 232 520 0.0 6 516 3 0 1 233 444 0.0 6 438 5 1 234 457 0.0 6 452 3 1 0 0 1 235 495 0.0 6 491 3 0 0 0 1 236 487 0.0 6 476 11 237 566 0.0 6 556 8 1 0 0 1 238 461 0.0 6 454 6 0 0 0 0 1 239 526 0.0 6 522 4 240 515 0.0 6 508 5 1 1 241 461 0.0 6 454 7 242 441 0.0 6 435 2 0 2 0 1 1 243 396 0.0 6 392 3 0 1 244 392 0.0 6 384 8 245 331 0.0 6 330 1 246 324 0.0 6 318 6 247 391 0.0 6 381 8 0 0 0 1 1 248 364 0.0 6 357 6 0 1 249 374 0.0 6 367 7 250 400 0.0 6 393 6 0 1 251 329 0.0 6 326 3 252 445 0.0 6 441 3 1 253 320 0.0 6 311 8 0 1 254 316 0.0 6 313 2 1 255 352 0.0 6 350 1 0 0 0 0 1 256 381 0.0 6 377 4 257 389 0.0 6 385 4 258 443 0.0 6 431 12 259 499 0.0 6 493 6 260 236 0.0 6 229 6 0 0 1 261 297 0.0 6 294 2 0 0 0 0 1 262 244 0.0 6 240 4 263 267 0.0 6 263 4 264 275 0.0 6 272 2 1 265 282 0.0 6 279 3 266 308 0.0 6 303 5 267 305 0.0 6 299 6 268 447 0.0 6 434 13 269 283 0.0 6 276 7 270 285 0.0 6 282 2 1 271 234 0.0 6 227 7 272 362 0.0 6 353 9 273 539 0.0 6 530 9 274 372 0.0 6 369 2 1 275 445 0.0 6 437 7 1 276 902 0.0 6 889 10 1 0 0 1 1 277 3169 0.0 6 3090 73 3 2 0 1 278 5298 0.0 6 5169 125 2 2 279 82 0.0 6 76 4 1 1 280 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 994 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 64 23 0.0 6 23 65 1 0.0 6 1 72 1 0.0 6 1 73 4 0.0 6 4 74 5 0.0 6 5 75 4 0.0 6 4 76 7 0.0 6 7 77 3 0.0 6 3 78 9 0.0 6 9 79 7 0.0 6 7 80 3 0.0 6 3 81 1 0.0 6 1 82 3 0.0 6 3 83 4 0.0 6 4 84 1 0.0 6 1 85 3 0.0 6 2 1 86 3 0.0 6 3 87 3 0.0 6 3 88 2 0.0 6 2 89 6 0.0 6 6 90 3 0.0 6 3 91 3 0.0 6 3 92 2 0.0 6 2 93 2 0.0 6 2 94 6 0.0 6 6 95 6 0.0 6 6 96 4 0.0 6 4 97 6 0.0 6 6 98 4 0.0 6 4 101 4 0.0 6 4 102 3 0.0 6 3 103 1 0.0 6 1 104 7 0.0 6 7 105 3 0.0 6 3 106 2 0.0 6 2 107 1 0.0 6 1 108 1 0.0 6 1 110 3 0.0 6 3 111 7 0.0 6 7 112 3 0.0 6 3 114 5 0.0 6 5 115 6 0.0 6 6 116 6 0.0 6 6 117 5 0.0 6 5 118 4 0.0 6 4 119 1 0.0 6 1 121 2 0.0 6 2 122 5 0.0 6 5 123 3 0.0 6 3 124 1 0.0 6 1 125 2 0.0 6 2 126 1 0.0 6 1 127 3 0.0 6 3 128 5 0.0 6 5 129 5 0.0 6 5 130 2 0.0 6 2 131 5 0.0 6 5 132 5 0.0 6 5 133 2 0.0 6 2 134 6 0.0 6 6 135 10 0.0 6 10 136 2 0.0 6 2 137 1 0.0 6 1 138 3 0.0 6 3 139 4 0.0 6 4 140 5 0.0 6 5 141 2 0.0 6 2 142 9 0.0 6 9 143 3 0.0 6 3 144 2 0.0 6 2 145 1 0.0 6 1 146 5 0.0 6 5 147 6 0.0 6 6 148 3 0.0 6 3 149 4 0.0 6 4 150 3 0.0 6 3 151 5 0.0 6 5 152 5 0.0 6 5 153 5 0.0 6 5 154 6 0.0 6 5 0 0 0 0 0 1 155 7 0.0 6 7 156 5 0.0 6 5 157 3 0.0 6 3 158 5 0.0 6 4 0 0 0 0 0 1 159 2 0.0 6 2 160 7 0.0 6 7 161 7 0.0 6 7 162 4 0.0 6 4 163 2 0.0 6 2 164 7 0.0 6 7 165 6 0.0 6 6 166 2 0.0 6 2 167 5 0.0 6 5 168 6 0.0 6 6 169 4 0.0 6 4 170 4 0.0 6 4 171 5 0.0 6 5 172 1 0.0 6 1 173 3 0.0 6 3 174 9 0.0 6 8 0 0 0 0 0 1 175 9 0.0 6 9 176 2 0.0 6 2 177 4 0.0 6 4 178 9 0.0 6 9 179 6 0.0 6 6 180 3 0.0 6 3 181 3 0.0 6 3 182 5 0.0 6 5 183 15 0.0 6 15 184 9 0.0 6 9 185 4 0.0 6 4 186 10 0.0 6 10 187 7 0.0 6 7 188 4 0.0 6 4 189 5 0.0 6 5 190 8 0.0 6 8 191 2 0.0 6 2 192 1 0.0 6 1 193 5 0.0 6 5 194 6 0.0 6 6 195 4 0.0 6 4 196 9 0.0 6 9 197 3 0.0 6 3 198 4 0.0 6 4 199 8 0.0 6 8 200 7 0.0 6 7 201 12 0.0 6 12 202 7 0.0 6 7 203 5 0.0 6 5 204 12 0.0 6 12 205 4 0.0 6 4 206 3 0.0 6 3 207 5 0.0 6 5 208 3 0.0 6 3 209 8 0.0 6 8 210 12 0.0 6 12 211 11 0.0 6 10 0 0 0 0 0 1 212 5 0.0 6 5 213 9 0.0 6 9 214 6 0.0 6 6 215 7 0.0 6 7 216 3 0.0 6 3 217 2 0.0 6 2 218 7 0.0 6 7 219 5 0.0 6 5 220 3 0.0 6 3 221 2 0.0 6 2 222 13 0.0 6 13 223 10 0.0 6 10 224 4 0.0 6 4 225 5 0.0 6 5 226 3 0.0 6 3 227 6 0.0 6 6 228 10 0.0 6 10 229 12 0.0 6 12 230 5 0.0 6 5 231 9 0.0 6 9 232 8 0.0 6 8 233 9 0.0 6 9 234 3 0.0 6 3 235 1 0.0 6 1 236 4 0.0 6 4 237 6 0.0 6 6 238 7 0.0 6 7 239 5 0.0 6 5 240 6 0.0 6 6 241 4 0.0 6 4 242 4 0.0 6 4 243 5 0.0 6 5 244 5 0.0 6 5 245 7 0.0 6 7 246 5 0.0 6 5 247 2 0.0 6 2 248 1 0.0 6 1 249 10 0.0 6 10 250 11 0.0 6 11 251 3 0.0 6 3 252 2 0.0 6 2 253 2 0.0 6 2 254 4 0.0 6 4 255 7 0.0 6 7 256 5 0.0 6 5 257 6 0.0 6 6 258 4 0.0 6 4 259 5 0.0 6 5 260 6 0.0 6 6 261 1 0.0 6 1 262 3 0.0 6 3 263 4 0.0 6 4 264 7 0.0 6 7 265 3 0.0 6 3 266 2 0.0 6 2 268 5 0.0 6 5 269 3 0.0 6 3 270 1 0.0 6 1 272 1 0.0 6 1 273 1 0.0 6 1 274 2 0.0 6 2 275 1 0.0 6 1 276 1 0.0 6 1 277 1 0.0 6 1 278 2 0.0 6 2 279 4 0.0 6 4 280 5 0.0 6 5 281 1 0.0 6 1 282 4 0.0 6 4 284 2 0.0 6 2 285 2 0.0 6 2 286 2 0.0 6 2 287 1 0.0 6 1 288 1 0.0 6 1 292 1 0.0 6 1 294 1 0.0 6 1 295 3 0.0 6 3 296 2 0.0 6 2 297 2 0.0 6 2 301 1 0.0 6 1 310 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 59468 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 9 2 0.2 2 2 10 1 0.1 3 1 11 2 0.0 3 1 0 0 1 12 2 0.0 3 2 13 10 0.0 3 9 1 14 6 0.0 4 6 16 12 0.0 4 10 2 17 20 0.0 5 18 1 1 18 14 0.0 5 14 19 29 0.0 5 28 1 20 87 0.0 6 82 5 21 51 0.0 6 50 1 22 32 0.0 6 31 1 23 25 0.0 6 24 0 0 0 0 1 24 25 0.0 6 24 1 25 18 0.0 6 17 0 1 26 15 0.0 6 15 27 22 0.0 6 19 3 28 12 0.0 6 11 1 29 15 0.0 6 15 30 19 0.0 6 19 31 10 0.0 6 10 32 6 0.0 6 6 33 6 0.0 6 5 0 0 1 34 16 0.0 6 15 1 35 7 0.0 6 6 1 36 11 0.0 6 11 37 11 0.0 6 9 2 38 12 0.0 6 11 1 39 14 0.0 6 13 1 40 9 0.0 6 9 41 16 0.0 6 16 42 18 0.0 6 17 1 43 19 0.0 6 16 3 44 12 0.0 6 12 45 13 0.0 6 13 46 10 0.0 6 10 47 19 0.0 6 19 48 19 0.0 6 19 49 16 0.0 6 15 1 50 27 0.0 6 23 4 51 22 0.0 6 21 0 0 0 1 52 13 0.0 6 11 1 0 0 1 53 17 0.0 6 15 1 0 1 54 18 0.0 6 18 55 25 0.0 6 22 3 56 23 0.0 6 20 2 1 57 20 0.0 6 20 58 16 0.0 6 16 59 21 0.0 6 19 2 60 17 0.0 6 16 1 61 27 0.0 6 25 2 62 30 0.0 6 30 63 25 0.0 6 25 64 22 0.0 6 19 3 65 37 0.0 6 36 1 66 35 0.0 6 34 1 67 32 0.0 6 30 2 68 37 0.0 6 36 1 69 107 0.0 6 103 3 1 70 57 0.0 6 56 1 71 70 0.0 6 69 1 72 47 0.0 6 43 4 73 39 0.0 6 38 1 74 39 0.0 6 37 2 75 43 0.0 6 42 1 76 38 0.0 6 34 4 77 34 0.0 6 32 2 78 52 0.0 6 49 3 79 35 0.0 6 32 3 80 48 0.0 6 38 8 0 0 0 1 1 81 35 0.0 6 33 2 82 79 0.0 6 74 4 0 1 83 68 0.0 6 64 4 84 70 0.0 6 64 6 85 71 0.0 6 70 0 0 0 0 0 1 86 53 0.0 6 50 3 87 41 0.0 6 37 3 1 88 46 0.0 6 43 1 1 1 89 43 0.0 6 40 3 90 58 0.0 6 56 2 91 58 0.0 6 55 2 1 92 73 0.0 6 71 2 93 64 0.0 6 60 3 0 0 1 94 66 0.0 6 59 6 1 95 89 0.0 6 86 3 96 68 0.0 6 63 5 97 72 0.0 6 68 3 0 0 0 0 1 98 94 0.0 6 93 1 99 83 0.0 6 80 3 100 94 0.0 6 91 3 101 76 0.0 6 75 1 102 90 0.0 6 87 3 103 119 0.0 6 113 6 104 102 0.0 6 98 4 105 112 0.0 6 112 106 180 0.0 6 175 4 0 0 0 0 1 107 126 0.0 6 120 6 108 107 0.0 6 106 1 109 118 0.0 6 114 3 1 110 112 0.0 6 109 3 111 102 0.0 6 97 5 112 153 0.0 6 149 3 1 113 152 0.0 6 148 4 114 173 0.0 6 170 3 115 150 0.0 6 147 3 116 155 0.0 6 149 6 117 184 0.0 6 178 6 118 174 0.0 6 171 3 119 200 0.0 6 191 8 0 1 120 187 0.0 6 184 2 0 1 121 179 0.0 6 173 4 0 1 1 122 188 0.0 6 178 8 1 0 1 123 184 0.0 6 178 6 124 217 0.0 6 208 6 0 1 1 1 125 220 0.0 6 211 9 126 249 0.0 6 240 8 0 0 1 127 269 0.0 6 256 10 1 0 0 1 1 128 299 0.0 6 290 9 129 264 0.0 6 250 14 130 337 0.0 6 327 9 0 0 0 1 131 370 0.0 6 356 12 0 0 0 2 132 353 0.0 6 344 7 0 0 1 0 1 133 439 0.0 6 425 14 134 410 0.0 6 395 10 2 1 1 0 1 135 412 0.0 6 391 17 0 3 0 1 136 456 0.0 6 441 12 0 0 2 0 1 137 451 0.0 6 436 12 0 2 0 0 1 138 351 0.0 6 340 10 1 139 428 0.0 6 417 10 0 0 1 140 412 0.0 6 400 12 141 526 0.0 6 507 16 0 2 0 1 142 676 0.0 6 661 13 0 0 1 1 143 783 0.0 6 769 11 0 2 0 0 1 144 744 0.0 6 716 26 0 1 1 145 729 0.0 6 704 23 1 0 0 1 146 787 0.0 6 763 22 1 0 1 147 729 0.0 6 706 17 4 1 0 0 1 148 808 0.0 6 786 16 0 0 2 0 4 149 880 0.0 6 855 23 0 0 2 150 886 0.0 6 852 28 3 0 1 1 1 151 974 0.0 6 945 23 1 2 0 1 2 152 1312 0.0 6 1281 28 0 1 2 153 917 0.0 6 893 22 1 0 0 0 1 154 1147 0.0 6 1113 28 2 2 2 155 1123 0.0 6 1095 26 0 1 1 156 924 0.0 6 888 29 2 2 1 1 1 157 1004 0.0 6 980 21 1 0 1 0 1 158 963 0.0 6 931 31 1 159 912 0.0 6 892 16 0 0 0 3 1 160 914 0.0 6 889 17 3 0 2 1 2 161 770 0.0 6 748 19 1 1 1 162 845 0.0 6 827 16 1 0 1 163 746 0.0 6 727 16 0 1 0 2 164 630 0.0 6 618 10 0 0 0 1 1 165 662 0.0 6 654 7 0 0 0 0 1 166 667 0.0 6 646 16 0 3 2 167 592 0.0 6 572 18 1 0 0 1 168 763 0.0 6 748 12 2 1 169 586 0.0 6 576 10 170 744 0.0 6 732 11 0 0 1 171 658 0.0 6 648 8 1 0 1 172 604 0.0 6 586 15 1 0 1 1 173 713 0.0 6 701 10 1 0 0 1 174 732 0.0 6 717 14 0 0 0 0 1 175 802 0.0 6 779 21 1 1 176 647 0.0 6 637 9 0 0 0 1 177 661 0.0 6 644 17 178 648 0.0 6 630 18 179 565 0.0 6 553 7 2 1 1 1 180 526 0.0 6 512 14 181 437 0.0 6 431 6 182 331 0.0 6 321 8 1 0 1 183 427 0.0 6 417 9 0 0 0 1 184 328 0.0 6 320 6 2 185 531 0.0 6 515 14 1 0 1 186 780 0.0 6 768 11 1 187 663 0.0 6 643 17 2 1 188 598 0.0 6 583 15 189 510 0.0 6 502 8 190 562 0.0 6 547 15 191 455 0.0 6 444 10 0 0 0 0 1 192 411 0.0 6 395 15 0 0 1 193 444 0.0 6 429 12 2 0 0 1 194 385 0.0 6 370 12 2 0 1 195 484 0.0 6 470 11 1 0 2 196 441 0.0 6 431 9 0 0 0 1 197 277 0.0 6 269 8 198 675 0.0 6 653 22 199 364 0.0 6 355 9 200 395 0.0 6 385 10 201 289 0.0 6 284 5 202 322 0.0 6 307 14 0 0 0 0 1 203 269 0.0 6 261 8 204 260 0.0 6 251 8 0 0 0 0 1 205 209 0.0 6 204 3 2 206 198 0.0 6 195 2 0 0 1 207 197 0.0 6 192 4 1 208 275 0.0 6 264 8 2 1 209 122 0.0 6 117 5 210 158 0.0 6 154 3 1 211 207 0.0 6 203 4 212 114 0.0 6 112 2 213 76 0.0 6 74 2 214 125 0.0 6 122 3 215 167 0.0 6 161 6 216 306 0.0 6 302 4 217 143 0.0 6 141 2 218 150 0.0 6 143 7 219 132 0.0 6 124 8 220 129 0.0 6 127 2 221 78 0.0 6 72 6 222 120 0.0 6 113 6 0 0 1 223 113 0.0 6 109 4 224 121 0.0 6 120 1 225 86 0.0 6 83 3 226 92 0.0 6 91 1 227 156 0.0 6 146 10 228 117 0.0 6 110 7 229 165 0.0 6 162 3 230 352 0.0 6 343 9 231 110 0.0 6 105 5 232 178 0.0 6 170 8 233 46 0.0 6 42 4 234 110 0.0 6 110 235 42 0.0 6 41 1 236 50 0.0 6 49 1 237 50 0.0 6 50 238 17 0.0 6 15 2 239 16 0.0 6 16 240 22 0.0 6 20 2 241 32 0.0 6 29 3 242 21 0.0 6 21 243 36 0.0 6 35 0 1 244 24 0.0 6 23 1 245 50 0.0 6 48 2 246 32 0.0 6 32 247 27 0.0 6 26 1 248 19 0.0 6 17 2 249 23 0.0 6 22 0 1 250 9 0.0 6 9 251 33 0.0 6 32 1 252 17 0.0 6 16 1 253 18 0.0 6 18 254 7 0.0 6 7 255 4 0.0 6 4 256 2 0.0 6 2 257 1 0.0 6 1 263 1 0.0 6 1 265 1 0.0 6 1 269 1 0.0 6 1 278 1 0.0 6 1 279 2 0.0 6 2 280 18 0.0 6 18 281 80 0.0 6 78 1 0 0 0 0 1 282 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 627 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 50 2 0.0 6 1 1 54 1 0.0 6 1 67 1 0.0 6 1 68 1 0.0 6 1 69 2 0.0 6 2 70 4 0.0 6 4 71 9 0.0 6 9 72 8 0.0 6 8 73 4 0.0 6 4 74 4 0.0 6 4 75 7 0.0 6 7 76 9 0.0 6 8 0 0 0 1 77 4 0.0 6 4 78 7 0.0 6 7 79 3 0.0 6 3 81 3 0.0 6 3 82 4 0.0 6 4 83 2 0.0 6 2 84 2 0.0 6 2 85 4 0.0 6 4 86 2 0.0 6 2 87 2 0.0 6 2 88 5 0.0 6 5 89 1 0.0 6 1 90 5 0.0 6 5 91 7 0.0 6 7 92 1 0.0 6 1 93 5 0.0 6 5 94 3 0.0 6 3 95 4 0.0 6 4 96 13 0.0 6 13 97 3 0.0 6 3 98 3 0.0 6 3 99 2 0.0 6 2 100 2 0.0 6 2 101 2 0.0 6 2 102 4 0.0 6 4 103 4 0.0 6 4 104 4 0.0 6 4 105 6 0.0 6 6 106 5 0.0 6 5 107 6 0.0 6 6 108 19 0.0 6 19 109 3 0.0 6 3 110 2 0.0 6 2 111 6 0.0 6 6 112 11 0.0 6 11 113 3 0.0 6 3 114 4 0.0 6 4 115 2 0.0 6 2 116 2 0.0 6 2 117 10 0.0 6 10 118 2 0.0 6 2 119 3 0.0 6 3 120 2 0.0 6 2 121 2 0.0 6 2 122 4 0.0 6 4 123 2 0.0 6 2 124 3 0.0 6 3 125 5 0.0 6 5 126 8 0.0 6 8 127 3 0.0 6 3 128 2 0.0 6 2 129 5 0.0 6 4 0 0 0 1 130 5 0.0 6 5 131 1 0.0 6 1 132 7 0.0 6 7 133 6 0.0 6 6 134 1 0.0 6 1 135 9 0.0 6 9 136 3 0.0 6 3 137 6 0.0 6 6 138 4 0.0 6 4 139 11 0.0 6 11 140 4 0.0 6 4 141 4 0.0 6 4 142 2 0.0 6 2 143 3 0.0 6 3 144 6 0.0 6 6 145 2 0.0 6 2 146 4 0.0 6 4 147 3 0.0 6 3 148 8 0.0 6 8 149 2 0.0 6 2 150 2 0.0 6 2 151 4 0.0 6 4 152 10 0.0 6 10 153 4 0.0 6 4 154 3 0.0 6 3 155 6 0.0 6 6 156 2 0.0 6 2 157 4 0.0 6 4 158 4 0.0 6 4 159 8 0.0 6 8 160 9 0.0 6 9 161 4 0.0 6 4 162 5 0.0 6 5 163 4 0.0 6 4 164 7 0.0 6 7 165 7 0.0 6 7 166 1 0.0 6 1 167 2 0.0 6 2 168 4 0.0 6 4 169 4 0.0 6 4 170 2 0.0 6 2 171 6 0.0 6 6 172 8 0.0 6 8 173 5 0.0 6 5 174 5 0.0 6 5 175 6 0.0 6 6 176 5 0.0 6 5 177 2 0.0 6 2 178 5 0.0 6 5 179 3 0.0 6 3 180 4 0.0 6 4 181 12 0.0 6 12 182 1 0.0 6 1 183 6 0.0 6 6 184 4 0.0 6 4 185 6 0.0 6 6 186 4 0.0 6 4 187 3 0.0 6 3 188 4 0.0 6 4 189 5 0.0 6 5 190 5 0.0 6 5 191 2 0.0 6 2 192 2 0.0 6 2 193 3 0.0 6 3 194 2 0.0 6 2 195 2 0.0 6 2 196 1 0.0 6 1 197 4 0.0 6 4 198 1 0.0 6 1 199 6 0.0 6 6 200 6 0.0 6 6 201 2 0.0 6 2 202 1 0.0 6 1 205 2 0.0 6 2 206 1 0.0 6 1 207 2 0.0 6 2 208 1 0.0 6 1 209 1 0.0 6 1 210 4 0.0 6 4 211 1 0.0 6 1 212 2 0.0 6 2 213 1 0.0 6 1 215 1 0.0 6 1 216 1 0.0 6 1 217 1 0.0 6 1 218 1 0.0 6 1 219 2 0.0 6 2 220 5 0.0 6 5 222 1 0.0 6 1 224 1 0.0 6 1 226 1 0.0 6 1 227 1 0.0 6 1 228 1 0.0 6 1 233 1 0.0 6 1 236 1 0.0 6 1 238 1 0.0 6 1 240 1 0.0 6 1 245 1 0.0 6 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_02_73_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_02_300_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_02_73_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_02_300_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.21 s (33 µs/read; 1.79 M reads/minute). === Summary === Total read pairs processed: 66,101 Read 1 with adapter: 66,099 (100.0%) Read 2 with adapter: 66,101 (100.0%) == Read fate breakdown == Pairs that were too short: 23 (0.0%) Pairs discarded as untrimmed: 2 (0.0%) Pairs written (passing filters): 66,076 (100.0%) Total basepairs processed: 29,760,747 bp Read 1: 16,845,326 bp Read 2: 12,915,421 bp Total written (filtered): 4,296,286 bp (14.4%) Read 1: 2,228,075 bp Read 2: 2,068,211 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 64948 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 8 258.2 1 5 3 6 1 16.1 1 0 0 1 7 3 4.0 2 1 0 2 8 1 1.0 2 1 9 1 0.3 2 1 13 1 0.0 3 1 18 1 0.0 5 1 19 1 0.0 5 1 20 68 0.0 6 66 2 21 39 0.0 6 38 1 22 34 0.0 6 33 1 23 25 0.0 6 25 24 15 0.0 6 14 1 25 17 0.0 6 17 26 8 0.0 6 8 27 11 0.0 6 10 0 0 0 0 1 28 7 0.0 6 6 1 29 12 0.0 6 12 30 5 0.0 6 5 31 7 0.0 6 6 1 32 10 0.0 6 8 2 33 4 0.0 6 4 34 3 0.0 6 2 1 35 2 0.0 6 2 36 5 0.0 6 5 37 8 0.0 6 8 38 3 0.0 6 3 39 4 0.0 6 4 40 6 0.0 6 6 41 12 0.0 6 11 1 42 5 0.0 6 5 43 4 0.0 6 4 44 11 0.0 6 10 1 45 8 0.0 6 7 0 0 1 46 4 0.0 6 4 47 6 0.0 6 6 48 7 0.0 6 7 49 8 0.0 6 8 50 3 0.0 6 3 51 4 0.0 6 4 52 1 0.0 6 1 53 4 0.0 6 3 1 54 2 0.0 6 2 55 6 0.0 6 5 0 1 56 4 0.0 6 3 1 57 8 0.0 6 7 1 58 6 0.0 6 5 1 59 4 0.0 6 4 60 5 0.0 6 5 61 6 0.0 6 6 62 4 0.0 6 4 63 7 0.0 6 6 1 64 3 0.0 6 3 65 5 0.0 6 4 1 66 8 0.0 6 8 67 4 0.0 6 4 68 7 0.0 6 7 69 5 0.0 6 5 70 6 0.0 6 6 71 5 0.0 6 5 72 10 0.0 6 9 1 73 8 0.0 6 7 1 74 7 0.0 6 7 75 7 0.0 6 7 76 8 0.0 6 8 77 8 0.0 6 8 78 9 0.0 6 9 79 6 0.0 6 6 80 10 0.0 6 10 81 10 0.0 6 10 82 8 0.0 6 7 1 83 11 0.0 6 11 84 8 0.0 6 8 85 7 0.0 6 6 1 86 13 0.0 6 12 1 87 7 0.0 6 7 88 10 0.0 6 10 89 19 0.0 6 16 3 90 6 0.0 6 5 0 0 0 0 1 91 3 0.0 6 3 92 10 0.0 6 9 1 93 7 0.0 6 7 94 6 0.0 6 6 95 12 0.0 6 12 96 12 0.0 6 12 97 7 0.0 6 7 98 8 0.0 6 8 99 13 0.0 6 13 100 5 0.0 6 5 101 21 0.0 6 20 1 102 11 0.0 6 11 103 8 0.0 6 8 104 17 0.0 6 13 3 1 105 19 0.0 6 18 1 106 8 0.0 6 6 2 107 11 0.0 6 11 108 7 0.0 6 7 109 17 0.0 6 16 1 110 10 0.0 6 9 0 0 0 0 1 111 22 0.0 6 20 2 112 16 0.0 6 15 1 113 13 0.0 6 13 114 27 0.0 6 26 1 115 19 0.0 6 19 116 17 0.0 6 16 1 117 20 0.0 6 19 1 118 22 0.0 6 19 2 1 119 14 0.0 6 13 1 120 16 0.0 6 16 121 23 0.0 6 22 0 1 122 29 0.0 6 29 123 21 0.0 6 20 1 124 18 0.0 6 18 125 23 0.0 6 22 0 0 0 0 1 126 29 0.0 6 29 127 30 0.0 6 30 128 25 0.0 6 24 0 1 129 25 0.0 6 24 1 130 29 0.0 6 29 131 24 0.0 6 24 132 37 0.0 6 35 1 0 0 0 1 133 31 0.0 6 31 134 37 0.0 6 35 2 135 40 0.0 6 39 1 136 51 0.0 6 51 137 34 0.0 6 31 3 138 33 0.0 6 33 139 38 0.0 6 38 140 33 0.0 6 33 141 56 0.0 6 56 142 140 0.0 6 138 2 143 197 0.0 6 196 1 144 247 0.0 6 246 1 145 157 0.0 6 157 146 149 0.0 6 149 147 253 0.0 6 251 2 148 239 0.0 6 239 149 180 0.0 6 178 1 0 0 0 0 1 150 159 0.0 6 157 1 0 0 0 1 151 176 0.0 6 176 152 131 0.0 6 130 1 153 124 0.0 6 124 154 94 0.0 6 93 1 155 121 0.0 6 119 2 156 161 0.0 6 160 1 157 146 0.0 6 144 2 158 146 0.0 6 145 1 159 150 0.0 6 147 3 160 130 0.0 6 130 161 166 0.0 6 163 3 162 178 0.0 6 177 1 163 201 0.0 6 200 1 164 223 0.0 6 219 4 165 233 0.0 6 232 1 166 179 0.0 6 176 3 167 177 0.0 6 175 2 168 203 0.0 6 203 169 182 0.0 6 180 2 170 208 0.0 6 205 3 171 253 0.0 6 252 0 1 172 182 0.0 6 180 1 0 0 0 1 173 199 0.0 6 198 1 174 230 0.0 6 229 0 0 0 0 1 175 219 0.0 6 216 3 176 192 0.0 6 191 1 177 221 0.0 6 219 1 0 0 0 0 1 178 218 0.0 6 216 1 1 179 205 0.0 6 202 2 0 0 0 1 180 486 0.0 6 486 181 282 0.0 6 279 3 182 287 0.0 6 284 3 183 281 0.0 6 278 3 184 286 0.0 6 283 2 0 1 185 315 0.0 6 306 8 1 186 328 0.0 6 323 5 187 377 0.0 6 374 3 188 293 0.0 6 289 4 189 345 0.0 6 344 1 190 296 0.0 6 292 3 1 191 332 0.0 6 331 0 0 0 0 1 192 403 0.0 6 396 7 193 450 0.0 6 445 5 194 551 0.0 6 542 9 195 651 0.0 6 645 6 196 695 0.0 6 691 3 1 197 727 0.0 6 714 11 1 1 198 590 0.0 6 586 3 1 199 539 0.0 6 534 4 0 0 0 1 200 542 0.0 6 540 1 1 201 532 0.0 6 528 4 202 612 0.0 6 605 7 203 547 0.0 6 543 4 204 726 0.0 6 715 11 205 752 0.0 6 742 10 206 578 0.0 6 573 3 2 207 724 0.0 6 717 7 208 599 0.0 6 591 8 209 544 0.0 6 540 2 1 1 210 677 0.0 6 673 2 2 211 785 0.0 6 775 10 212 641 0.0 6 636 3 0 1 1 213 840 0.0 6 836 4 214 884 0.0 6 877 4 2 1 215 775 0.0 6 764 10 1 216 696 0.0 6 688 5 1 0 1 0 1 217 744 0.0 6 735 7 2 218 667 0.0 6 660 6 0 0 1 219 602 0.0 6 601 1 220 566 0.0 6 561 5 221 524 0.0 6 515 7 1 0 0 0 1 222 582 0.0 6 574 7 1 223 647 0.0 6 638 9 224 776 0.0 6 761 14 0 0 0 1 225 784 0.0 6 769 15 226 656 0.0 6 649 6 1 227 616 0.0 6 607 9 228 569 0.0 6 565 3 1 229 575 0.0 6 565 10 230 585 0.0 6 578 7 231 582 0.0 6 577 5 232 580 0.0 6 576 4 233 580 0.0 6 572 7 0 0 0 0 1 234 483 0.0 6 478 5 235 529 0.0 6 525 3 0 0 0 1 236 525 0.0 6 520 4 0 0 0 0 1 237 589 0.0 6 583 5 1 238 488 0.0 6 483 4 0 0 0 1 239 495 0.0 6 489 6 240 548 0.0 6 540 8 241 493 0.0 6 483 9 1 242 485 0.0 6 478 7 243 514 0.0 6 509 4 0 0 1 244 370 0.0 6 368 2 245 313 0.0 6 309 4 246 315 0.0 6 312 3 247 357 0.0 6 350 7 248 323 0.0 6 317 5 1 249 405 0.0 6 401 4 250 325 0.0 6 320 5 251 261 0.0 6 258 3 252 387 0.0 6 382 5 253 294 0.0 6 287 7 254 332 0.0 6 323 9 255 277 0.0 6 272 5 256 346 0.0 6 336 10 257 336 0.0 6 334 2 258 410 0.0 6 404 4 1 0 0 1 259 261 0.0 6 253 8 260 316 0.0 6 309 7 261 399 0.0 6 390 9 262 233 0.0 6 226 7 263 283 0.0 6 272 11 264 220 0.0 6 216 4 265 249 0.0 6 244 3 2 266 195 0.0 6 191 3 1 267 333 0.0 6 325 7 1 268 254 0.0 6 245 7 1 0 1 269 253 0.0 6 244 9 270 478 0.0 6 472 5 1 271 250 0.0 6 247 2 1 272 463 0.0 6 448 13 1 1 273 571 0.0 6 557 14 274 655 0.0 6 637 18 275 747 0.0 6 730 16 1 276 662 0.0 6 647 14 1 277 1152 0.0 6 1129 21 1 0 0 0 1 278 7320 0.0 6 7129 188 1 2 279 163 0.0 6 153 10 280 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1151 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 64 17 0.0 6 17 72 8 0.0 6 8 73 5 0.0 6 5 74 3 0.0 6 3 75 6 0.0 6 6 76 7 0.0 6 7 77 7 0.0 6 7 78 7 0.0 6 7 79 6 0.0 6 6 80 3 0.0 6 3 81 5 0.0 6 5 82 8 0.0 6 8 83 3 0.0 6 3 84 3 0.0 6 3 85 2 0.0 6 2 86 5 0.0 6 5 87 4 0.0 6 4 88 1 0.0 6 1 89 2 0.0 6 2 90 1 0.0 6 1 91 6 0.0 6 6 92 1 0.0 6 1 93 6 0.0 6 6 94 3 0.0 6 3 95 5 0.0 6 5 96 8 0.0 6 8 97 5 0.0 6 5 98 4 0.0 6 4 99 5 0.0 6 5 100 3 0.0 6 3 101 3 0.0 6 3 102 5 0.0 6 5 103 1 0.0 6 1 104 2 0.0 6 2 105 3 0.0 6 3 106 3 0.0 6 3 107 7 0.0 6 7 108 1 0.0 6 1 109 5 0.0 6 5 110 5 0.0 6 5 111 16 0.0 6 16 112 5 0.0 6 5 113 1 0.0 6 1 114 2 0.0 6 2 115 7 0.0 6 7 116 3 0.0 6 3 117 4 0.0 6 4 118 4 0.0 6 4 119 4 0.0 6 4 120 6 0.0 6 6 121 3 0.0 6 3 122 2 0.0 6 2 123 3 0.0 6 3 124 6 0.0 6 6 125 9 0.0 6 9 126 3 0.0 6 3 127 4 0.0 6 4 128 5 0.0 6 5 129 5 0.0 6 5 130 6 0.0 6 6 131 6 0.0 6 6 132 4 0.0 6 4 133 6 0.0 6 6 134 5 0.0 6 5 135 11 0.0 6 11 136 6 0.0 6 6 137 4 0.0 6 4 138 6 0.0 6 6 139 6 0.0 6 6 140 9 0.0 6 9 141 9 0.0 6 9 142 5 0.0 6 5 143 8 0.0 6 8 144 9 0.0 6 9 145 7 0.0 6 7 146 9 0.0 6 9 147 5 0.0 6 5 148 6 0.0 6 6 149 7 0.0 6 7 150 9 0.0 6 9 151 6 0.0 6 6 152 7 0.0 6 7 153 8 0.0 6 8 154 6 0.0 6 6 155 12 0.0 6 11 1 156 4 0.0 6 4 157 6 0.0 6 6 158 8 0.0 6 8 159 9 0.0 6 9 160 8 0.0 6 8 161 5 0.0 6 4 1 162 2 0.0 6 2 163 8 0.0 6 8 164 5 0.0 6 5 165 11 0.0 6 11 166 8 0.0 6 8 167 8 0.0 6 8 168 13 0.0 6 13 169 7 0.0 6 7 170 6 0.0 6 6 171 8 0.0 6 8 172 8 0.0 6 8 173 7 0.0 6 7 174 5 0.0 6 5 175 13 0.0 6 13 176 9 0.0 6 9 177 3 0.0 6 3 178 8 0.0 6 8 179 17 0.0 6 17 180 9 0.0 6 9 181 3 0.0 6 3 182 7 0.0 6 6 1 183 15 0.0 6 15 184 5 0.0 6 5 185 8 0.0 6 8 186 6 0.0 6 6 187 8 0.0 6 8 188 12 0.0 6 12 189 7 0.0 6 7 190 7 0.0 6 7 191 8 0.0 6 8 192 16 0.0 6 16 193 12 0.0 6 12 194 9 0.0 6 9 195 9 0.0 6 9 196 6 0.0 6 6 197 8 0.0 6 8 198 11 0.0 6 11 199 12 0.0 6 12 200 10 0.0 6 10 201 9 0.0 6 9 202 8 0.0 6 8 203 2 0.0 6 2 204 6 0.0 6 6 205 11 0.0 6 11 206 5 0.0 6 5 207 15 0.0 6 15 208 8 0.0 6 8 209 8 0.0 6 8 210 9 0.0 6 9 211 2 0.0 6 2 212 8 0.0 6 8 213 7 0.0 6 7 214 6 0.0 6 6 215 10 0.0 6 10 216 5 0.0 6 5 217 3 0.0 6 3 218 4 0.0 6 4 219 9 0.0 6 9 220 7 0.0 6 7 221 7 0.0 6 7 222 4 0.0 6 4 223 8 0.0 6 8 224 9 0.0 6 9 225 5 0.0 6 5 226 6 0.0 6 6 227 5 0.0 6 5 228 8 0.0 6 8 229 3 0.0 6 3 231 5 0.0 6 5 232 7 0.0 6 7 233 1 0.0 6 1 234 3 0.0 6 3 235 8 0.0 6 8 236 10 0.0 6 10 237 2 0.0 6 2 238 2 0.0 6 2 239 4 0.0 6 4 240 5 0.0 6 5 241 5 0.0 6 5 242 3 0.0 6 3 243 3 0.0 6 3 244 2 0.0 6 2 245 5 0.0 6 5 246 4 0.0 6 4 247 1 0.0 6 1 248 5 0.0 6 5 249 4 0.0 6 4 250 4 0.0 6 4 251 1 0.0 6 1 252 2 0.0 6 2 253 1 0.0 6 1 254 2 0.0 6 2 255 3 0.0 6 3 256 2 0.0 6 2 258 1 0.0 6 1 259 2 0.0 6 2 260 3 0.0 6 3 261 1 0.0 6 1 262 3 0.0 6 3 263 2 0.0 6 2 264 1 0.0 6 1 265 1 0.0 6 1 267 2 0.0 6 2 269 1 0.0 6 1 271 2 0.0 6 2 272 2 0.0 6 2 275 2 0.0 6 2 276 1 0.0 6 1 278 1 0.0 6 1 289 1 0.0 6 1 296 1 0.0 6 1 300 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 65208 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 16.1 1 1 7 1 4.0 2 1 8 1 1.0 2 1 10 3 0.1 3 3 12 7 0.0 3 6 1 13 8 0.0 3 8 14 12 0.0 4 12 15 2 0.0 4 1 1 16 3 0.0 4 3 17 32 0.0 5 29 2 0 1 18 18 0.0 5 16 1 1 19 19 0.0 5 16 2 1 20 61 0.0 6 57 4 21 42 0.0 6 39 3 22 27 0.0 6 26 1 23 18 0.0 6 15 2 1 24 19 0.0 6 17 2 25 17 0.0 6 16 1 26 26 0.0 6 23 2 1 27 15 0.0 6 15 28 10 0.0 6 10 29 10 0.0 6 8 2 30 12 0.0 6 11 1 31 12 0.0 6 12 32 9 0.0 6 9 33 8 0.0 6 7 1 34 16 0.0 6 15 1 35 12 0.0 6 10 1 0 1 36 13 0.0 6 12 1 37 13 0.0 6 12 1 38 8 0.0 6 8 39 7 0.0 6 7 40 12 0.0 6 12 41 10 0.0 6 8 1 0 0 1 42 13 0.0 6 11 2 43 10 0.0 6 7 2 1 44 19 0.0 6 19 45 13 0.0 6 11 2 46 23 0.0 6 22 1 47 18 0.0 6 18 48 18 0.0 6 15 1 1 0 0 1 49 32 0.0 6 30 2 50 18 0.0 6 18 51 22 0.0 6 19 3 52 25 0.0 6 24 1 53 21 0.0 6 19 2 54 18 0.0 6 15 2 0 0 1 55 20 0.0 6 17 3 56 22 0.0 6 18 4 57 26 0.0 6 22 3 1 58 24 0.0 6 23 1 59 24 0.0 6 21 3 60 17 0.0 6 17 61 17 0.0 6 16 1 62 16 0.0 6 13 2 0 0 1 63 15 0.0 6 14 1 64 36 0.0 6 32 4 65 25 0.0 6 23 2 66 28 0.0 6 25 3 67 48 0.0 6 44 4 68 124 0.0 6 112 11 0 0 0 1 69 64 0.0 6 61 3 70 58 0.0 6 55 3 71 35 0.0 6 33 1 0 0 1 72 40 0.0 6 37 3 73 36 0.0 6 33 3 74 27 0.0 6 25 2 75 40 0.0 6 37 3 76 33 0.0 6 31 2 77 29 0.0 6 26 3 78 36 0.0 6 33 2 0 0 0 0 1 79 36 0.0 6 32 3 0 0 0 1 80 41 0.0 6 39 1 1 81 49 0.0 6 46 3 82 46 0.0 6 42 3 0 0 1 83 61 0.0 6 56 4 1 84 64 0.0 6 60 4 85 61 0.0 6 57 4 86 44 0.0 6 42 0 2 87 45 0.0 6 39 5 1 88 39 0.0 6 36 3 89 45 0.0 6 41 3 0 0 0 1 90 49 0.0 6 45 4 91 61 0.0 6 58 3 92 66 0.0 6 63 3 93 67 0.0 6 65 1 1 94 96 0.0 6 92 3 1 95 64 0.0 6 61 2 0 0 0 1 96 70 0.0 6 61 4 2 1 1 1 97 66 0.0 6 64 2 98 67 0.0 6 61 6 99 76 0.0 6 74 2 100 48 0.0 6 47 1 101 85 0.0 6 81 4 102 98 0.0 6 96 2 103 85 0.0 6 82 3 104 108 0.0 6 102 5 0 1 105 156 0.0 6 149 6 1 106 93 0.0 6 89 4 107 101 0.0 6 99 1 1 108 85 0.0 6 83 1 1 109 84 0.0 6 81 3 110 106 0.0 6 104 1 1 111 97 0.0 6 90 6 0 0 0 1 112 100 0.0 6 98 2 113 116 0.0 6 110 5 1 114 108 0.0 6 104 3 0 0 1 115 129 0.0 6 125 4 116 147 0.0 6 141 5 0 0 0 0 1 117 126 0.0 6 121 4 1 118 154 0.0 6 150 4 119 148 0.0 6 143 3 0 0 1 1 120 164 0.0 6 160 4 121 133 0.0 6 126 7 122 180 0.0 6 176 4 123 134 0.0 6 130 3 0 0 0 0 1 124 168 0.0 6 161 7 125 214 0.0 6 212 2 126 233 0.0 6 226 6 0 1 127 226 0.0 6 222 3 1 128 217 0.0 6 215 1 1 129 224 0.0 6 219 4 1 130 268 0.0 6 261 6 0 0 1 131 286 0.0 6 278 7 0 0 1 132 323 0.0 6 314 9 133 392 0.0 6 376 14 0 0 0 1 1 134 372 0.0 6 367 5 135 465 0.0 6 454 11 136 399 0.0 6 388 10 0 0 1 137 374 0.0 6 365 7 1 0 0 0 1 138 357 0.0 6 348 7 1 0 0 1 139 416 0.0 6 403 10 0 0 0 0 3 140 455 0.0 6 446 7 0 1 0 0 1 141 522 0.0 6 509 13 142 658 0.0 6 645 13 143 674 0.0 6 664 9 1 144 675 0.0 6 656 18 1 145 631 0.0 6 621 6 0 0 1 2 1 146 775 0.0 6 760 15 147 723 0.0 6 710 11 0 1 0 0 1 148 736 0.0 6 722 12 0 1 1 149 942 0.0 6 925 13 0 1 3 150 1029 0.0 6 1004 22 1 1 0 0 1 151 1115 0.0 6 1093 20 0 0 0 1 1 152 1000 0.0 6 971 25 1 2 0 0 1 153 1005 0.0 6 974 25 3 0 1 0 2 154 1109 0.0 6 1089 19 0 0 0 1 155 895 0.0 6 879 13 1 0 2 156 965 0.0 6 954 8 1 0 1 1 157 848 0.0 6 830 17 0 0 0 0 1 158 965 0.0 6 940 21 1 0 2 0 1 159 844 0.0 6 835 8 0 0 0 0 1 160 870 0.0 6 852 17 0 0 1 161 789 0.0 6 772 11 1 2 1 0 2 162 802 0.0 6 786 12 3 0 0 0 1 163 719 0.0 6 708 9 1 0 0 1 164 802 0.0 6 784 17 0 0 1 165 703 0.0 6 692 8 1 1 0 0 1 166 760 0.0 6 747 13 167 804 0.0 6 785 18 0 0 1 168 846 0.0 6 831 14 0 0 0 0 1 169 844 0.0 6 826 17 1 170 730 0.0 6 722 6 0 1 0 0 1 171 848 0.0 6 830 15 0 1 1 1 172 747 0.0 6 730 15 1 1 173 771 0.0 6 760 10 0 0 0 1 174 867 0.0 6 853 13 1 175 954 0.0 6 933 20 0 1 176 761 0.0 6 751 8 2 177 896 0.0 6 879 11 2 3 1 178 678 0.0 6 666 11 1 179 751 0.0 6 734 16 1 180 633 0.0 6 621 12 181 773 0.0 6 749 20 1 2 1 182 461 0.0 6 452 8 0 1 183 646 0.0 6 630 14 1 1 184 658 0.0 6 647 8 1 0 1 0 1 185 835 0.0 6 819 16 186 1055 0.0 6 1040 13 1 0 0 0 1 187 915 0.0 6 897 17 1 188 879 0.0 6 857 22 189 697 0.0 6 679 17 0 1 190 1032 0.0 6 1009 22 0 0 1 191 880 0.0 6 855 24 0 0 1 192 747 0.0 6 734 13 193 504 0.0 6 488 12 1 0 0 1 2 194 576 0.0 6 560 16 195 663 0.0 6 644 19 196 1051 0.0 6 1018 30 2 0 1 197 615 0.0 6 601 13 1 198 654 0.0 6 633 21 199 1074 0.0 6 1044 29 1 200 510 0.0 6 492 16 2 201 566 0.0 6 549 14 1 0 1 0 1 202 425 0.0 6 414 8 2 1 203 331 0.0 6 322 9 204 307 0.0 6 302 5 205 222 0.0 6 214 8 206 177 0.0 6 175 2 207 171 0.0 6 166 4 0 0 1 208 233 0.0 6 229 4 209 191 0.0 6 187 4 210 159 0.0 6 154 5 211 152 0.0 6 151 1 212 179 0.0 6 176 3 213 161 0.0 6 160 1 214 161 0.0 6 159 2 215 209 0.0 6 198 11 216 315 0.0 6 306 9 217 167 0.0 6 162 4 1 218 210 0.0 6 205 5 219 148 0.0 6 145 2 0 0 1 220 134 0.0 6 127 7 221 206 0.0 6 199 6 1 222 164 0.0 6 161 2 0 0 1 223 226 0.0 6 216 10 224 84 0.0 6 83 1 225 95 0.0 6 94 1 226 60 0.0 6 59 1 227 121 0.0 6 115 6 228 65 0.0 6 64 1 229 107 0.0 6 101 6 230 36 0.0 6 36 231 30 0.0 6 30 232 72 0.0 6 68 4 233 54 0.0 6 51 3 234 27 0.0 6 26 1 235 28 0.0 6 27 1 236 15 0.0 6 13 2 237 11 0.0 6 11 238 11 0.0 6 11 239 11 0.0 6 11 240 23 0.0 6 23 241 18 0.0 6 17 1 242 41 0.0 6 41 243 31 0.0 6 30 1 244 22 0.0 6 21 1 245 13 0.0 6 12 1 246 8 0.0 6 8 247 5 0.0 6 5 248 13 0.0 6 13 249 15 0.0 6 15 250 2 0.0 6 2 251 4 0.0 6 4 252 10 0.0 6 10 253 4 0.0 6 4 254 2 0.0 6 2 278 2 0.0 6 2 279 11 0.0 6 10 1 280 70 0.0 6 69 1 281 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 893 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 3 73 1032.8 0 73 55 1 0.0 6 1 59 1 0.0 6 1 69 1 0.0 6 1 70 3 0.0 6 3 71 5 0.0 6 5 72 5 0.0 6 5 73 7 0.0 6 7 74 6 0.0 6 6 75 5 0.0 6 5 76 8 0.0 6 8 77 3 0.0 6 3 78 2 0.0 6 2 79 5 0.0 6 5 80 6 0.0 6 6 81 5 0.0 6 4 1 82 3 0.0 6 3 83 2 0.0 6 2 84 5 0.0 6 5 85 6 0.0 6 6 86 4 0.0 6 4 87 6 0.0 6 6 88 5 0.0 6 5 89 2 0.0 6 2 90 3 0.0 6 3 91 4 0.0 6 4 92 10 0.0 6 10 93 4 0.0 6 4 94 6 0.0 6 5 0 0 0 1 95 2 0.0 6 2 96 6 0.0 6 6 97 5 0.0 6 5 98 5 0.0 6 5 99 6 0.0 6 6 100 2 0.0 6 2 101 2 0.0 6 2 102 5 0.0 6 5 103 5 0.0 6 5 105 4 0.0 6 4 106 2 0.0 6 2 107 3 0.0 6 3 108 11 0.0 6 11 109 13 0.0 6 13 110 5 0.0 6 5 111 7 0.0 6 7 112 8 0.0 6 8 113 5 0.0 6 5 114 4 0.0 6 4 115 3 0.0 6 3 116 5 0.0 6 5 117 6 0.0 6 6 118 5 0.0 6 5 119 2 0.0 6 2 120 5 0.0 6 5 121 3 0.0 6 3 122 8 0.0 6 8 123 4 0.0 6 4 124 6 0.0 6 6 125 3 0.0 6 3 126 4 0.0 6 4 127 7 0.0 6 7 128 11 0.0 6 11 129 6 0.0 6 6 130 2 0.0 6 2 131 5 0.0 6 5 132 4 0.0 6 4 133 9 0.0 6 8 1 134 6 0.0 6 6 135 5 0.0 6 5 136 8 0.0 6 8 137 7 0.0 6 7 138 11 0.0 6 11 139 7 0.0 6 7 140 11 0.0 6 10 0 0 0 1 141 5 0.0 6 5 142 9 0.0 6 9 143 7 0.0 6 7 144 8 0.0 6 8 145 8 0.0 6 8 146 4 0.0 6 4 147 10 0.0 6 10 148 11 0.0 6 11 149 4 0.0 6 4 150 8 0.0 6 8 151 8 0.0 6 8 152 9 0.0 6 9 153 5 0.0 6 5 154 4 0.0 6 4 155 5 0.0 6 5 156 12 0.0 6 12 157 5 0.0 6 5 158 12 0.0 6 11 0 0 0 1 159 3 0.0 6 3 160 5 0.0 6 5 161 12 0.0 6 12 162 13 0.0 6 13 163 8 0.0 6 8 164 10 0.0 6 10 165 11 0.0 6 11 166 9 0.0 6 9 167 2 0.0 6 2 168 6 0.0 6 6 169 6 0.0 6 6 170 6 0.0 6 6 171 3 0.0 6 3 172 7 0.0 6 7 173 12 0.0 6 12 174 5 0.0 6 5 175 7 0.0 6 7 176 8 0.0 6 8 177 10 0.0 6 10 178 4 0.0 6 4 179 9 0.0 6 9 180 5 0.0 6 5 181 15 0.0 6 14 1 182 3 0.0 6 3 183 1 0.0 6 1 184 8 0.0 6 8 185 11 0.0 6 11 186 6 0.0 6 6 187 6 0.0 6 6 188 11 0.0 6 10 1 189 7 0.0 6 7 190 3 0.0 6 3 191 6 0.0 6 6 193 4 0.0 6 4 194 3 0.0 6 3 195 4 0.0 6 4 196 3 0.0 6 2 0 0 0 1 197 7 0.0 6 7 198 2 0.0 6 2 199 6 0.0 6 6 200 5 0.0 6 5 201 2 0.0 6 2 202 2 0.0 6 2 203 1 0.0 6 1 204 1 0.0 6 1 205 3 0.0 6 3 206 3 0.0 6 3 208 1 0.0 6 1 210 1 0.0 6 1 211 2 0.0 6 2 212 2 0.0 6 1 1 213 2 0.0 6 2 214 1 0.0 6 1 216 1 0.0 6 1 217 2 0.0 6 2 218 1 0.0 6 1 219 1 0.0 6 1 221 1 0.0 6 1 224 1 0.0 6 1 225 1 0.0 6 1 229 2 0.0 6 2 231 1 0.0 6 1 235 1 0.0 6 1 239 1 0.0 6 1 241 1 0.0 6 1 244 1 0.0 6 1 248 1 0.0 6 1 250 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_03_74_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_03_301_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_03_74_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_03_301_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.08 s (24 µs/read; 2.55 M reads/minute). === Summary === Total read pairs processed: 88,319 Read 1 with adapter: 88,312 (100.0%) Read 2 with adapter: 88,245 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 81 (0.1%) Pairs written (passing filters): 88,238 (99.9%) Total basepairs processed: 42,880,087 bp Read 1: 24,255,712 bp Read 2: 18,624,375 bp Total written (filtered): 5,750,875 bp (13.4%) Read 1: 2,923,101 bp Read 2: 2,827,774 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 88083 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 5 1380.0 0 4 1 4 1 345.0 1 0 1 6 1 21.6 1 0 0 1 7 2 5.4 2 2 9 5 0.3 2 4 1 14 1 0.0 4 1 15 4 0.0 4 4 16 5 0.0 4 5 17 2 0.0 5 2 19 2 0.0 5 1 1 20 31 0.0 6 31 21 27 0.0 6 25 2 22 13 0.0 6 13 23 12 0.0 6 11 1 24 9 0.0 6 8 1 25 9 0.0 6 9 26 8 0.0 6 8 27 6 0.0 6 4 2 28 4 0.0 6 4 29 7 0.0 6 7 30 3 0.0 6 2 1 31 4 0.0 6 4 32 1 0.0 6 0 1 33 3 0.0 6 3 34 4 0.0 6 3 1 35 1 0.0 6 1 36 3 0.0 6 2 1 37 7 0.0 6 6 0 0 1 38 10 0.0 6 10 39 5 0.0 6 2 2 0 1 40 5 0.0 6 4 1 41 1 0.0 6 1 43 3 0.0 6 3 44 8 0.0 6 8 45 6 0.0 6 6 46 5 0.0 6 5 47 1 0.0 6 1 48 1 0.0 6 1 49 3 0.0 6 3 50 7 0.0 6 7 53 3 0.0 6 3 54 6 0.0 6 6 55 4 0.0 6 3 1 56 8 0.0 6 7 1 57 6 0.0 6 5 1 58 4 0.0 6 4 59 4 0.0 6 4 60 5 0.0 6 4 0 1 61 4 0.0 6 4 62 10 0.0 6 8 2 63 5 0.0 6 5 64 8 0.0 6 7 1 65 4 0.0 6 4 66 4 0.0 6 3 0 1 67 3 0.0 6 3 69 3 0.0 6 2 1 70 2 0.0 6 2 71 2 0.0 6 1 1 72 10 0.0 6 10 73 1 0.0 6 1 74 2 0.0 6 1 1 75 1 0.0 6 1 77 6 0.0 6 6 78 2 0.0 6 1 1 79 1 0.0 6 1 80 3 0.0 6 2 0 0 1 81 1 0.0 6 1 82 2 0.0 6 1 0 0 0 1 83 2 0.0 6 1 1 84 3 0.0 6 3 85 6 0.0 6 6 86 5 0.0 6 5 87 5 0.0 6 4 1 89 11 0.0 6 10 1 90 2 0.0 6 2 92 4 0.0 6 4 93 2 0.0 6 2 95 2 0.0 6 2 96 5 0.0 6 5 97 5 0.0 6 4 1 98 7 0.0 6 7 99 2 0.0 6 2 100 3 0.0 6 3 101 13 0.0 6 13 102 7 0.0 6 7 103 5 0.0 6 5 104 3 0.0 6 3 105 6 0.0 6 6 106 7 0.0 6 5 2 107 17 0.0 6 16 1 108 13 0.0 6 13 109 8 0.0 6 8 110 5 0.0 6 5 111 4 0.0 6 3 1 112 16 0.0 6 16 113 9 0.0 6 9 114 16 0.0 6 14 2 115 13 0.0 6 12 1 116 9 0.0 6 9 117 5 0.0 6 4 1 118 3 0.0 6 2 1 119 23 0.0 6 22 0 1 120 9 0.0 6 9 121 15 0.0 6 15 122 22 0.0 6 20 2 123 24 0.0 6 23 1 124 23 0.0 6 23 125 35 0.0 6 33 2 126 12 0.0 6 12 127 16 0.0 6 16 128 31 0.0 6 30 1 129 12 0.0 6 12 130 10 0.0 6 10 131 5 0.0 6 5 132 4 0.0 6 4 133 16 0.0 6 16 134 17 0.0 6 15 2 135 29 0.0 6 28 1 136 25 0.0 6 25 137 67 0.0 6 63 4 138 42 0.0 6 42 139 30 0.0 6 29 1 140 52 0.0 6 46 6 141 36 0.0 6 35 1 142 58 0.0 6 57 1 143 210 0.0 6 207 3 144 140 0.0 6 137 3 145 231 0.0 6 228 2 1 146 153 0.0 6 150 1 1 1 147 104 0.0 6 103 1 148 126 0.0 6 124 1 0 1 149 264 0.0 6 260 4 150 148 0.0 6 142 6 151 140 0.0 6 137 3 152 118 0.0 6 113 5 153 107 0.0 6 105 2 154 86 0.0 6 85 1 155 59 0.0 6 59 156 98 0.0 6 96 2 157 78 0.0 6 78 158 68 0.0 6 65 3 159 110 0.0 6 105 5 160 108 0.0 6 106 1 1 161 117 0.0 6 116 1 162 121 0.0 6 115 6 163 89 0.0 6 89 164 99 0.0 6 97 2 165 70 0.0 6 69 1 166 105 0.0 6 101 3 0 1 167 64 0.0 6 62 1 1 168 82 0.0 6 79 2 1 169 65 0.0 6 62 3 170 59 0.0 6 57 2 171 95 0.0 6 94 1 172 92 0.0 6 90 2 173 96 0.0 6 92 4 174 84 0.0 6 81 3 175 208 0.0 6 205 2 1 176 130 0.0 6 127 3 177 156 0.0 6 154 2 178 97 0.0 6 96 1 179 104 0.0 6 97 7 180 117 0.0 6 117 181 208 0.0 6 203 5 182 175 0.0 6 172 3 183 141 0.0 6 139 2 184 223 0.0 6 218 5 185 266 0.0 6 253 13 186 388 0.0 6 378 10 187 239 0.0 6 232 7 188 173 0.0 6 164 9 189 184 0.0 6 177 7 190 211 0.0 6 205 6 191 203 0.0 6 199 4 192 274 0.0 6 269 5 193 340 0.0 6 329 11 194 330 0.0 6 326 4 195 427 0.0 6 411 14 2 196 463 0.0 6 452 11 197 353 0.0 6 345 8 198 411 0.0 6 401 10 199 227 0.0 6 224 3 200 286 0.0 6 279 7 201 289 0.0 6 278 10 0 1 202 398 0.0 6 386 10 1 0 1 203 489 0.0 6 484 4 1 204 432 0.0 6 421 11 205 668 0.0 6 653 15 206 498 0.0 6 490 6 1 1 207 689 0.0 6 676 13 208 564 0.0 6 552 12 209 463 0.0 6 452 11 210 493 0.0 6 486 7 211 297 0.0 6 289 7 0 1 212 276 0.0 6 265 11 213 445 0.0 6 435 9 1 214 488 0.0 6 475 13 215 348 0.0 6 339 9 216 440 0.0 6 421 18 0 1 217 667 0.0 6 655 12 218 410 0.0 6 404 6 219 394 0.0 6 385 8 1 220 381 0.0 6 371 10 221 343 0.0 6 334 7 2 222 657 0.0 6 644 13 223 239 0.0 6 230 9 224 332 0.0 6 317 13 2 225 560 0.0 6 535 23 1 0 0 1 226 1384 0.0 6 1357 25 1 0 1 227 989 0.0 6 956 32 1 228 864 0.0 6 847 16 1 229 766 0.0 6 744 22 230 811 0.0 6 790 21 231 509 0.0 6 494 14 1 232 866 0.0 6 851 15 233 897 0.0 6 884 12 1 234 722 0.0 6 707 15 235 483 0.0 6 471 12 236 394 0.0 6 385 9 237 403 0.0 6 393 9 1 238 586 0.0 6 577 8 1 239 617 0.0 6 591 25 1 240 799 0.0 6 774 25 241 1182 0.0 6 1150 32 242 1125 0.0 6 1093 32 243 1059 0.0 6 1036 22 1 244 669 0.0 6 647 20 1 0 0 1 245 1113 0.0 6 1090 22 1 246 458 0.0 6 448 10 247 701 0.0 6 684 16 1 248 344 0.0 6 331 12 1 249 1179 0.0 6 1146 32 1 250 678 0.0 6 648 27 2 1 251 1000 0.0 6 969 31 252 764 0.0 6 752 9 3 253 500 0.0 6 481 16 3 254 1397 0.0 6 1372 25 255 871 0.0 6 847 24 256 634 0.0 6 615 19 257 929 0.0 6 907 22 258 1136 0.0 6 1107 27 1 0 0 1 259 1356 0.0 6 1314 42 260 1392 0.0 6 1365 26 1 261 729 0.0 6 709 18 2 262 1597 0.0 6 1560 34 2 0 0 1 263 2217 0.0 6 2170 46 0 1 264 1399 0.0 6 1363 36 265 1636 0.0 6 1605 30 1 266 965 0.0 6 931 32 2 267 1518 0.0 6 1489 29 268 1491 0.0 6 1467 23 1 269 929 0.0 6 891 36 2 270 417 0.0 6 401 16 271 1201 0.0 6 1162 37 2 272 3568 0.0 6 3506 58 4 273 1717 0.0 6 1671 44 2 274 1965 0.0 6 1906 56 3 275 1987 0.0 6 1920 62 4 1 276 3326 0.0 6 3241 80 4 1 277 8327 0.0 6 8163 164 278 979 0.0 6 838 123 15 3 279 134 0.0 6 126 7 1 280 34 0.0 6 31 2 1 281 4 0.0 6 4 283 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 229 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 2 0.0 6 2 64 35 0.0 6 34 1 72 2 0.0 6 2 73 4 0.0 6 4 74 2 0.0 6 2 75 12 0.0 6 12 76 2 0.0 6 2 77 1 0.0 6 1 78 4 0.0 6 4 79 6 0.0 6 6 80 1 0.0 6 1 81 1 0.0 6 1 82 1 0.0 6 1 83 1 0.0 6 1 85 1 0.0 6 1 86 1 0.0 6 1 87 2 0.0 6 2 88 1 0.0 6 1 89 3 0.0 6 3 90 1 0.0 6 1 91 1 0.0 6 1 93 1 0.0 6 1 94 1 0.0 6 1 95 1 0.0 6 1 96 3 0.0 6 3 97 1 0.0 6 1 101 1 0.0 6 1 102 2 0.0 6 2 103 6 0.0 6 6 105 1 0.0 6 1 107 2 0.0 6 2 109 1 0.0 6 1 110 1 0.0 6 1 111 3 0.0 6 3 112 1 0.0 6 1 115 1 0.0 6 1 116 2 0.0 6 2 117 2 0.0 6 2 118 1 0.0 6 1 120 1 0.0 6 1 122 1 0.0 6 1 123 1 0.0 6 1 125 1 0.0 6 1 128 1 0.0 6 1 129 3 0.0 6 3 131 2 0.0 6 2 133 1 0.0 6 1 134 1 0.0 6 1 135 2 0.0 6 2 136 1 0.0 6 1 138 1 0.0 6 1 139 2 0.0 6 2 140 4 0.0 6 4 141 2 0.0 6 2 142 6 0.0 6 6 143 1 0.0 6 1 146 2 0.0 6 2 150 2 0.0 6 2 151 2 0.0 6 2 152 1 0.0 6 1 153 1 0.0 6 1 154 2 0.0 6 2 156 1 0.0 6 1 157 2 0.0 6 2 158 3 0.0 6 3 161 1 0.0 6 1 162 1 0.0 6 1 164 2 0.0 6 2 165 1 0.0 6 1 166 1 0.0 6 1 167 1 0.0 6 1 168 1 0.0 6 1 169 1 0.0 6 1 170 1 0.0 6 1 171 1 0.0 6 1 172 3 0.0 6 3 173 4 0.0 6 4 174 3 0.0 6 3 176 1 0.0 6 1 177 1 0.0 6 1 178 1 0.0 6 1 183 1 0.0 6 1 184 2 0.0 6 2 186 2 0.0 6 2 187 1 0.0 6 1 188 1 0.0 6 1 191 1 0.0 6 1 195 2 0.0 6 2 196 2 0.0 6 2 197 1 0.0 6 1 198 2 0.0 6 2 202 1 0.0 6 1 203 1 0.0 6 1 204 2 0.0 6 2 205 2 0.0 6 2 208 1 0.0 6 1 210 2 0.0 6 2 213 2 0.0 6 2 216 1 0.0 6 1 218 1 0.0 6 1 219 1 0.0 6 1 220 1 0.0 6 1 224 2 0.0 6 2 231 1 0.0 6 1 235 1 0.0 6 1 236 2 0.0 6 2 238 1 0.0 6 1 252 1 0.0 6 1 254 1 0.0 6 1 255 1 0.0 6 1 256 1 0.0 6 1 262 1 0.0 6 1 263 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 88102 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1380.0 0 0 1 6 3 21.6 1 2 1 7 1 5.4 2 1 9 4 0.3 2 4 10 8 0.1 3 8 11 2 0.0 3 2 12 2 0.0 3 1 1 13 11 0.0 3 9 1 0 1 14 7 0.0 4 6 1 15 2 0.0 4 1 1 16 9 0.0 4 8 1 17 17 0.0 5 15 2 18 17 0.0 5 14 2 1 19 12 0.0 5 9 3 20 63 0.0 6 59 3 0 0 1 21 72 0.0 6 67 4 1 22 43 0.0 6 38 4 1 23 30 0.0 6 27 3 24 31 0.0 6 28 3 25 36 0.0 6 36 26 20 0.0 6 20 27 15 0.0 6 15 28 22 0.0 6 20 2 29 19 0.0 6 14 5 30 23 0.0 6 21 2 31 12 0.0 6 11 0 1 32 14 0.0 6 14 33 12 0.0 6 10 2 34 16 0.0 6 15 0 0 1 35 11 0.0 6 10 1 36 13 0.0 6 10 2 0 0 1 37 14 0.0 6 14 38 8 0.0 6 7 1 39 15 0.0 6 15 40 11 0.0 6 10 1 41 8 0.0 6 8 42 7 0.0 6 6 1 43 18 0.0 6 17 1 44 20 0.0 6 16 4 45 11 0.0 6 10 1 46 31 0.0 6 27 3 1 47 22 0.0 6 19 3 48 15 0.0 6 13 2 49 25 0.0 6 22 3 50 21 0.0 6 20 1 51 12 0.0 6 11 1 52 34 0.0 6 30 4 53 26 0.0 6 24 2 54 35 0.0 6 32 3 55 25 0.0 6 21 3 1 56 18 0.0 6 15 3 57 32 0.0 6 29 2 1 58 35 0.0 6 31 4 59 19 0.0 6 18 0 1 60 13 0.0 6 13 61 16 0.0 6 13 3 62 9 0.0 6 7 2 63 19 0.0 6 16 3 64 16 0.0 6 15 1 65 9 0.0 6 8 1 66 42 0.0 6 35 7 67 184 0.0 6 173 10 1 68 52 0.0 6 48 4 69 58 0.0 6 57 1 70 22 0.0 6 17 5 71 37 0.0 6 35 1 1 72 44 0.0 6 40 4 73 13 0.0 6 9 4 74 25 0.0 6 24 1 75 16 0.0 6 15 1 76 38 0.0 6 35 2 0 0 1 77 20 0.0 6 17 3 78 46 0.0 6 41 4 1 79 40 0.0 6 37 3 80 27 0.0 6 23 2 2 81 32 0.0 6 31 1 82 36 0.0 6 34 1 0 0 1 83 67 0.0 6 59 7 1 84 46 0.0 6 46 85 33 0.0 6 32 1 86 18 0.0 6 14 4 87 69 0.0 6 67 2 88 31 0.0 6 27 3 1 89 52 0.0 6 47 3 2 90 53 0.0 6 48 4 1 91 44 0.0 6 37 6 0 0 0 1 92 45 0.0 6 39 6 93 57 0.0 6 52 5 94 111 0.0 6 105 6 95 85 0.0 6 77 7 1 96 92 0.0 6 83 9 97 57 0.0 6 54 1 1 0 1 98 69 0.0 6 64 4 0 1 99 57 0.0 6 54 3 100 60 0.0 6 53 7 101 62 0.0 6 57 5 102 78 0.0 6 74 3 1 103 110 0.0 6 105 5 104 87 0.0 6 83 3 1 105 79 0.0 6 68 9 1 0 1 106 40 0.0 6 36 4 107 60 0.0 6 55 5 108 44 0.0 6 41 3 109 59 0.0 6 58 1 110 67 0.0 6 61 5 1 111 78 0.0 6 75 3 112 64 0.0 6 61 3 113 49 0.0 6 43 6 114 87 0.0 6 82 4 0 0 1 115 79 0.0 6 70 8 1 116 93 0.0 6 89 4 117 65 0.0 6 61 4 118 82 0.0 6 76 6 119 80 0.0 6 79 1 120 140 0.0 6 134 4 0 0 1 0 1 121 107 0.0 6 99 7 0 0 1 122 133 0.0 6 119 10 4 123 114 0.0 6 110 3 0 0 1 124 194 0.0 6 181 11 1 0 1 125 152 0.0 6 143 8 0 0 1 126 148 0.0 6 142 5 0 0 1 127 160 0.0 6 157 3 128 114 0.0 6 108 6 129 128 0.0 6 124 4 130 142 0.0 6 138 4 131 232 0.0 6 214 15 1 2 132 327 0.0 6 313 11 2 1 133 240 0.0 6 231 8 1 134 360 0.0 6 344 14 0 0 2 135 446 0.0 6 429 16 1 136 201 0.0 6 196 5 137 263 0.0 6 250 13 138 219 0.0 6 211 8 139 281 0.0 6 273 8 140 377 0.0 6 362 12 0 0 3 141 492 0.0 6 478 11 2 0 0 0 1 142 533 0.0 6 513 17 2 0 1 143 516 0.0 6 498 18 144 610 0.0 6 592 18 145 365 0.0 6 348 15 2 146 392 0.0 6 378 11 1 0 1 1 147 435 0.0 6 420 11 2 1 1 148 418 0.0 6 405 9 1 2 1 149 644 0.0 6 621 23 150 956 0.0 6 919 33 2 2 151 415 0.0 6 398 15 1 0 1 152 969 0.0 6 938 27 2 0 2 153 636 0.0 6 621 12 1 0 2 154 676 0.0 6 648 25 3 155 699 0.0 6 679 19 1 156 505 0.0 6 489 15 1 157 875 0.0 6 845 28 1 1 158 877 0.0 6 850 25 1 0 1 159 420 0.0 6 403 14 2 0 1 160 726 0.0 6 704 22 161 490 0.0 6 474 14 0 1 1 162 488 0.0 6 473 12 1 0 1 0 1 163 652 0.0 6 633 17 0 0 0 2 164 616 0.0 6 596 19 1 165 569 0.0 6 553 13 2 1 166 827 0.0 6 805 20 2 167 534 0.0 6 503 30 1 168 1495 0.0 6 1451 40 2 0 2 169 385 0.0 6 374 9 1 0 0 0 1 170 702 0.0 6 679 21 2 171 1657 0.0 6 1605 48 0 2 1 0 1 172 487 0.0 6 468 16 1 1 0 1 173 556 0.0 6 546 10 174 847 0.0 6 808 33 3 1 2 175 1228 0.0 6 1191 34 2 0 1 176 880 0.0 6 852 27 0 0 0 0 1 177 839 0.0 6 815 23 0 0 1 178 744 0.0 6 713 31 179 925 0.0 6 886 35 1 0 1 2 180 814 0.0 6 777 34 3 181 1070 0.0 6 1031 35 2 0 2 182 357 0.0 6 339 16 2 183 944 0.0 6 913 29 2 184 1110 0.0 6 1077 33 185 1172 0.0 6 1130 42 186 1962 0.0 6 1898 62 0 1 1 187 1783 0.0 6 1722 59 1 1 188 1687 0.0 6 1634 49 2 0 2 189 1707 0.0 6 1641 64 1 1 190 2080 0.0 6 2024 50 4 0 2 191 2148 0.0 6 2075 67 2 2 0 2 192 1257 0.0 6 1223 29 2 1 2 193 778 0.0 6 736 40 2 194 1407 0.0 6 1366 40 1 195 2020 0.0 6 1924 91 2 1 1 0 1 196 4417 0.0 6 4302 107 3 0 4 1 197 1094 0.0 6 1058 33 2 0 1 198 2098 0.0 6 2017 78 2 0 1 199 4021 0.0 6 3910 109 0 0 2 200 1012 0.0 6 978 32 1 0 1 201 1629 0.0 6 1585 41 0 0 2 1 202 784 0.0 6 760 22 2 203 759 0.0 6 739 18 1 0 1 204 1043 0.0 6 1015 24 3 0 0 0 1 205 412 0.0 6 404 7 0 0 0 1 206 346 0.0 6 334 11 0 0 1 207 262 0.0 6 252 10 208 377 0.0 6 367 10 209 410 0.0 6 394 13 1 0 2 210 353 0.0 6 343 10 211 283 0.0 6 275 8 212 514 0.0 6 495 19 213 306 0.0 6 296 10 214 881 0.0 6 854 26 0 1 215 680 0.0 6 654 23 1 1 1 216 987 0.0 6 964 21 0 1 0 1 217 354 0.0 6 342 11 0 0 1 218 348 0.0 6 339 9 219 280 0.0 6 271 8 0 1 220 125 0.0 6 122 3 221 379 0.0 6 371 7 1 222 725 0.0 6 700 24 0 0 1 223 1007 0.0 6 978 29 224 235 0.0 6 225 8 1 0 0 0 1 225 443 0.0 6 430 13 226 216 0.0 6 212 4 227 269 0.0 6 260 8 1 228 173 0.0 6 169 1 0 1 2 229 386 0.0 6 374 11 0 0 1 230 227 0.0 6 221 5 0 0 1 231 299 0.0 6 295 4 232 424 0.0 6 412 11 1 233 241 0.0 6 236 5 234 116 0.0 6 115 1 235 52 0.0 6 50 2 236 10 0.0 6 9 1 237 21 0.0 6 21 238 27 0.0 6 26 1 239 36 0.0 6 35 1 240 95 0.0 6 91 4 241 131 0.0 6 128 3 242 162 0.0 6 156 6 243 165 0.0 6 165 244 101 0.0 6 100 1 245 32 0.0 6 31 0 1 246 29 0.0 6 27 2 247 16 0.0 6 14 2 248 48 0.0 6 44 4 249 7 0.0 6 5 2 250 16 0.0 6 16 251 23 0.0 6 23 252 10 0.0 6 10 253 1 0.0 6 1 270 1 0.0 6 1 276 1 0.0 6 1 277 9 0.0 6 9 278 72 0.0 6 71 1 279 34 0.0 6 34 280 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 143 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 53 1 0.0 6 1 71 2 0.0 6 2 72 1 0.0 6 1 73 6 0.0 6 6 74 3 0.0 6 3 75 4 0.0 6 4 76 2 0.0 6 2 77 3 0.0 6 3 78 5 0.0 6 5 79 2 0.0 6 2 80 1 0.0 6 1 81 2 0.0 6 2 83 4 0.0 6 4 85 1 0.0 6 1 86 2 0.0 6 2 87 1 0.0 6 1 88 2 0.0 6 2 89 1 0.0 6 1 90 1 0.0 6 1 91 1 0.0 6 1 92 1 0.0 6 1 93 1 0.0 6 1 96 1 0.0 6 1 97 2 0.0 6 2 98 2 0.0 6 2 99 1 0.0 6 1 101 1 0.0 6 1 105 3 0.0 6 3 106 1 0.0 6 1 107 1 0.0 6 1 110 3 0.0 6 3 111 2 0.0 6 2 117 1 0.0 6 1 118 1 0.0 6 1 121 1 0.0 6 1 123 2 0.0 6 2 124 3 0.0 6 3 125 2 0.0 6 2 128 3 0.0 6 3 129 3 0.0 6 3 131 4 0.0 6 3 1 132 3 0.0 6 3 133 2 0.0 6 2 134 1 0.0 6 1 137 1 0.0 6 1 138 3 0.0 6 3 139 2 0.0 6 2 141 1 0.0 6 1 142 2 0.0 6 2 143 1 0.0 6 1 146 4 0.0 6 4 147 1 0.0 6 1 148 1 0.0 6 1 149 2 0.0 6 2 150 2 0.0 6 2 151 1 0.0 6 1 152 1 0.0 6 1 157 2 0.0 6 2 161 3 0.0 6 3 162 1 0.0 6 1 166 1 0.0 6 1 167 3 0.0 6 3 168 1 0.0 6 1 169 1 0.0 6 1 170 2 0.0 6 2 174 1 0.0 6 1 176 1 0.0 6 1 179 1 0.0 6 1 182 1 0.0 6 1 183 1 0.0 6 1 186 1 0.0 6 1 187 2 0.0 6 2 188 1 0.0 6 1 189 1 0.0 6 1 190 1 0.0 6 1 195 1 0.0 6 1 198 2 0.0 6 2 201 1 0.0 6 1 203 1 0.0 6 1 206 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_04_75_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_04_302_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_04_75_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_04_302_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.21 s (612 µs/read; 0.10 M reads/minute). === Summary === Total read pairs processed: 341 Read 1 with adapter: 341 (100.0%) Read 2 with adapter: 341 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 0 (0.0%) Pairs written (passing filters): 341 (100.0%) Total basepairs processed: 148,013 bp Read 1: 85,629 bp Read 2: 62,384 bp Total written (filtered): 21,417 bp (14.5%) Read 1: 11,313 bp Read 2: 10,104 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 340 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 66 1 0.0 6 1 68 2 0.0 6 2 117 4 0.0 6 1 0 0 0 0 3 118 1 0.0 6 1 120 1 0.0 6 1 136 1 0.0 6 1 140 1 0.0 6 1 141 1 0.0 6 1 142 1 0.0 6 1 144 1 0.0 6 1 145 2 0.0 6 2 146 18 0.0 6 18 147 2 0.0 6 2 148 1 0.0 6 1 150 1 0.0 6 1 151 2 0.0 6 2 152 1 0.0 6 1 162 1 0.0 6 1 166 3 0.0 6 3 168 1 0.0 6 1 177 1 0.0 6 1 178 1 0.0 6 1 180 1 0.0 6 1 181 1 0.0 6 1 182 2 0.0 6 1 1 184 1 0.0 6 1 186 1 0.0 6 1 187 1 0.0 6 1 188 2 0.0 6 2 190 10 0.0 6 9 1 191 1 0.0 6 1 192 16 0.0 6 14 2 193 2 0.0 6 2 194 1 0.0 6 0 1 195 10 0.0 6 10 196 3 0.0 6 2 1 197 4 0.0 6 2 2 198 1 0.0 6 1 199 6 0.0 6 6 200 2 0.0 6 1 1 201 7 0.0 6 7 202 7 0.0 6 7 203 2 0.0 6 2 204 2 0.0 6 2 205 3 0.0 6 3 206 2 0.0 6 2 207 7 0.0 6 7 208 2 0.0 6 2 211 5 0.0 6 5 213 2 0.0 6 2 214 4 0.0 6 4 215 7 0.0 6 7 216 2 0.0 6 2 217 4 0.0 6 4 218 3 0.0 6 3 219 2 0.0 6 2 220 3 0.0 6 3 221 5 0.0 6 5 222 8 0.0 6 7 1 224 1 0.0 6 1 225 13 0.0 6 13 226 9 0.0 6 9 227 5 0.0 6 5 228 5 0.0 6 5 229 2 0.0 6 1 1 232 2 0.0 6 2 238 1 0.0 6 1 239 1 0.0 6 0 1 241 2 0.0 6 2 242 2 0.0 6 1 1 244 2 0.0 6 2 245 1 0.0 6 1 246 3 0.0 6 3 248 1 0.0 6 1 249 2 0.0 6 2 250 1 0.0 6 1 251 1 0.0 6 1 252 3 0.0 6 2 1 253 1 0.0 6 1 256 1 0.0 6 1 257 1 0.0 6 1 258 3 0.0 6 3 259 2 0.0 6 1 1 260 1 0.0 6 1 261 2 0.0 6 2 262 1 0.0 6 1 263 4 0.0 6 4 264 2 0.0 6 2 265 1 0.0 6 1 266 3 0.0 6 2 1 268 1 0.0 6 1 269 1 0.0 6 1 272 4 0.0 6 4 273 3 0.0 6 3 274 4 0.0 6 4 275 1 0.0 6 1 276 8 0.0 6 8 277 29 0.0 6 29 278 20 0.0 6 19 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 79 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 338 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.7% G: 0.3% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 15 1 0.0 4 1 20 1 0.0 6 1 21 1 0.0 6 1 26 1 0.0 6 1 49 1 0.0 6 0 1 54 1 0.0 6 0 1 59 1 0.0 6 1 60 1 0.0 6 1 63 1 0.0 6 1 70 2 0.0 6 2 75 1 0.0 6 1 79 2 0.0 6 2 82 1 0.0 6 1 85 1 0.0 6 1 86 2 0.0 6 2 92 1 0.0 6 1 93 1 0.0 6 1 94 3 0.0 6 2 1 98 1 0.0 6 1 100 1 0.0 6 0 1 103 3 0.0 6 3 104 2 0.0 6 2 105 1 0.0 6 1 106 2 0.0 6 2 108 1 0.0 6 1 109 1 0.0 6 1 110 1 0.0 6 1 112 1 0.0 6 1 113 1 0.0 6 1 114 1 0.0 6 1 117 2 0.0 6 2 118 2 0.0 6 1 1 119 2 0.0 6 1 1 120 2 0.0 6 2 122 1 0.0 6 1 123 1 0.0 6 1 125 2 0.0 6 2 126 2 0.0 6 2 127 2 0.0 6 2 128 2 0.0 6 1 1 130 3 0.0 6 3 131 5 0.0 6 4 1 132 1 0.0 6 1 133 4 0.0 6 4 134 8 0.0 6 8 135 2 0.0 6 2 136 5 0.0 6 5 137 3 0.0 6 2 0 0 0 1 138 6 0.0 6 6 139 4 0.0 6 4 140 4 0.0 6 4 141 3 0.0 6 3 142 10 0.0 6 10 143 3 0.0 6 3 144 4 0.0 6 4 145 7 0.0 6 7 146 4 0.0 6 4 147 17 0.0 6 17 148 5 0.0 6 5 149 1 0.0 6 1 150 6 0.0 6 5 0 1 151 4 0.0 6 4 152 5 0.0 6 5 153 6 0.0 6 5 1 154 3 0.0 6 3 155 10 0.0 6 9 1 156 7 0.0 6 7 157 4 0.0 6 3 0 1 158 7 0.0 6 7 159 6 0.0 6 6 160 7 0.0 6 7 161 6 0.0 6 5 1 162 3 0.0 6 3 163 4 0.0 6 4 164 2 0.0 6 2 165 1 0.0 6 1 166 2 0.0 6 2 167 2 0.0 6 2 168 3 0.0 6 3 173 2 0.0 6 2 174 2 0.0 6 2 175 2 0.0 6 2 177 2 0.0 6 2 178 2 0.0 6 2 180 1 0.0 6 1 181 1 0.0 6 1 183 2 0.0 6 1 1 185 2 0.0 6 2 186 2 0.0 6 2 187 2 0.0 6 2 188 4 0.0 6 4 189 1 0.0 6 1 190 2 0.0 6 2 191 2 0.0 6 2 193 1 0.0 6 1 194 3 0.0 6 3 195 2 0.0 6 2 196 5 0.0 6 5 197 1 0.0 6 1 198 3 0.0 6 3 199 9 0.0 6 9 200 2 0.0 6 2 201 4 0.0 6 4 204 3 0.0 6 2 1 205 1 0.0 6 1 206 1 0.0 6 1 207 2 0.0 6 2 210 1 0.0 6 1 212 2 0.0 6 2 214 2 0.0 6 2 217 7 0.0 6 7 218 1 0.0 6 1 219 2 0.0 6 2 221 1 0.0 6 1 222 1 0.0 6 1 223 2 0.0 6 2 225 1 0.0 6 1 230 1 0.0 6 1 232 1 0.0 6 1 233 2 0.0 6 2 236 1 0.0 6 1 240 1 0.0 6 1 244 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 3 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 3 0.0 6 0 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_05_76_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_05_303_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_05_76_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_05_303_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.23 s (8775 µs/read; 0.01 M reads/minute). === Summary === Total read pairs processed: 26 Read 1 with adapter: 17 (65.4%) Read 2 with adapter: 16 (61.5%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 10 (38.5%) Pairs written (passing filters): 16 (61.5%) Total basepairs processed: 9,199 bp Read 1: 5,119 bp Read 2: 4,080 bp Total written (filtered): 1,042 bp (11.3%) Read 1: 562 bp Read 2: 480 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 16 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 93.8% G: 6.2% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 1 0.1 1 0 1 20 1 0.0 6 1 128 1 0.0 6 1 143 1 0.0 6 1 164 1 0.0 6 1 190 1 0.0 6 1 193 1 0.0 6 1 197 1 0.0 6 1 199 1 0.0 6 1 214 1 0.0 6 1 225 1 0.0 6 1 241 1 0.0 6 1 272 1 0.0 6 1 278 3 0.0 6 3 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 139 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 16 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 20 1 0.0 6 1 52 1 0.0 6 1 72 1 0.0 6 1 120 1 0.0 6 1 135 1 0.0 6 1 159 1 0.0 6 1 160 1 0.0 6 1 168 2 0.0 6 2 176 1 0.0 6 1 183 1 0.0 6 1 187 1 0.0 6 1 188 1 0.0 6 1 194 1 0.0 6 1 196 1 0.0 6 1 214 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 0 times This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_06_77_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_06_304_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_06_77_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_06_304_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.88 s (28 µs/read; 2.13 M reads/minute). === Summary === Total read pairs processed: 66,789 Read 1 with adapter: 66,784 (100.0%) Read 2 with adapter: 66,758 (100.0%) == Read fate breakdown == Pairs that were too short: 4 (0.0%) Pairs discarded as untrimmed: 36 (0.1%) Pairs written (passing filters): 66,749 (99.9%) Total basepairs processed: 32,740,128 bp Read 1: 18,550,627 bp Read 2: 14,189,501 bp Total written (filtered): 4,153,742 bp (12.7%) Read 1: 2,212,241 bp Read 2: 1,941,501 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 66529 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1043.6 0 0 2 4 2 260.9 1 2 5 1 65.2 1 1 6 1 16.3 1 0 0 1 7 2 4.1 2 2 11 1 0.0 3 1 17 1 0.0 5 0 0 0 1 18 2 0.0 5 2 20 16 0.0 6 15 1 21 9 0.0 6 9 22 8 0.0 6 8 23 3 0.0 6 3 24 7 0.0 6 7 25 10 0.0 6 9 1 26 6 0.0 6 6 27 6 0.0 6 6 28 1 0.0 6 1 29 1 0.0 6 1 31 3 0.0 6 3 32 1 0.0 6 0 0 1 33 1 0.0 6 1 34 6 0.0 6 6 35 3 0.0 6 3 36 1 0.0 6 1 37 2 0.0 6 1 1 38 3 0.0 6 2 0 1 39 1 0.0 6 1 40 3 0.0 6 3 42 2 0.0 6 2 43 1 0.0 6 1 44 11 0.0 6 10 1 45 5 0.0 6 4 1 46 5 0.0 6 4 0 1 47 1 0.0 6 1 48 1 0.0 6 0 1 49 1 0.0 6 1 50 2 0.0 6 2 51 2 0.0 6 2 53 1 0.0 6 1 54 6 0.0 6 6 55 3 0.0 6 2 1 56 2 0.0 6 2 57 4 0.0 6 4 58 1 0.0 6 1 59 2 0.0 6 2 60 3 0.0 6 1 2 61 2 0.0 6 1 1 62 5 0.0 6 5 63 2 0.0 6 2 64 4 0.0 6 3 1 65 7 0.0 6 4 2 1 66 1 0.0 6 1 67 3 0.0 6 3 68 1 0.0 6 1 69 5 0.0 6 4 1 70 2 0.0 6 2 71 1 0.0 6 1 72 4 0.0 6 4 73 5 0.0 6 5 74 1 0.0 6 1 76 2 0.0 6 2 77 3 0.0 6 3 78 3 0.0 6 3 79 1 0.0 6 1 80 2 0.0 6 2 81 2 0.0 6 2 84 2 0.0 6 2 85 4 0.0 6 3 1 86 8 0.0 6 7 1 87 6 0.0 6 6 88 1 0.0 6 1 89 5 0.0 6 5 90 2 0.0 6 2 91 2 0.0 6 2 92 2 0.0 6 2 93 4 0.0 6 4 95 3 0.0 6 3 96 2 0.0 6 2 97 4 0.0 6 4 98 4 0.0 6 4 99 2 0.0 6 2 100 6 0.0 6 6 101 9 0.0 6 9 102 8 0.0 6 8 103 7 0.0 6 7 104 4 0.0 6 4 105 4 0.0 6 4 106 4 0.0 6 4 107 5 0.0 6 5 108 8 0.0 6 7 1 109 5 0.0 6 5 110 4 0.0 6 3 0 0 0 0 1 111 4 0.0 6 4 112 6 0.0 6 6 113 5 0.0 6 4 1 114 2 0.0 6 2 115 7 0.0 6 7 116 5 0.0 6 5 117 3 0.0 6 3 118 6 0.0 6 6 119 7 0.0 6 7 120 11 0.0 6 11 121 10 0.0 6 10 122 15 0.0 6 15 123 13 0.0 6 13 124 11 0.0 6 11 125 15 0.0 6 15 126 10 0.0 6 10 127 8 0.0 6 8 128 18 0.0 6 16 2 129 8 0.0 6 8 130 10 0.0 6 10 131 7 0.0 6 6 1 132 6 0.0 6 6 133 10 0.0 6 10 134 18 0.0 6 18 135 27 0.0 6 26 1 136 18 0.0 6 18 137 42 0.0 6 38 4 138 31 0.0 6 30 1 139 20 0.0 6 19 1 140 61 0.0 6 61 141 47 0.0 6 47 142 103 0.0 6 102 1 143 167 0.0 6 167 144 37 0.0 6 37 145 55 0.0 6 51 3 1 146 81 0.0 6 81 147 83 0.0 6 81 2 148 60 0.0 6 58 2 149 71 0.0 6 69 2 150 100 0.0 6 96 2 2 151 51 0.0 6 50 1 152 45 0.0 6 44 0 0 0 1 153 57 0.0 6 55 2 154 43 0.0 6 42 1 155 44 0.0 6 43 1 156 65 0.0 6 65 157 65 0.0 6 65 158 39 0.0 6 39 159 72 0.0 6 70 2 160 68 0.0 6 67 1 161 68 0.0 6 68 162 49 0.0 6 46 3 163 107 0.0 6 105 2 164 54 0.0 6 54 165 87 0.0 6 86 1 166 111 0.0 6 109 2 167 43 0.0 6 43 168 76 0.0 6 75 1 169 48 0.0 6 47 1 170 50 0.0 6 48 2 171 70 0.0 6 64 6 172 64 0.0 6 63 1 173 62 0.0 6 60 2 174 119 0.0 6 119 175 138 0.0 6 134 3 1 176 150 0.0 6 145 5 177 108 0.0 6 108 178 96 0.0 6 94 2 179 65 0.0 6 63 2 180 96 0.0 6 95 1 181 80 0.0 6 77 3 182 97 0.0 6 94 3 183 116 0.0 6 112 4 184 130 0.0 6 127 3 185 192 0.0 6 187 4 1 186 175 0.0 6 170 5 187 119 0.0 6 114 2 0 1 0 1 1 188 106 0.0 6 105 0 0 0 0 0 1 189 209 0.0 6 206 3 190 170 0.0 6 166 4 191 288 0.0 6 285 3 192 168 0.0 6 160 8 193 287 0.0 6 279 8 194 300 0.0 6 295 5 195 274 0.0 6 268 6 196 375 0.0 6 367 8 197 244 0.0 6 237 7 198 244 0.0 6 236 7 1 199 140 0.0 6 137 1 1 0 0 1 200 170 0.0 6 163 6 0 0 0 0 1 201 212 0.0 6 208 4 202 251 0.0 6 243 8 203 305 0.0 6 299 4 1 1 204 248 0.0 6 243 5 205 365 0.0 6 358 5 0 0 1 0 1 206 276 0.0 6 269 7 207 439 0.0 6 430 7 2 208 414 0.0 6 399 14 1 209 379 0.0 6 372 5 1 0 0 1 210 420 0.0 6 416 4 211 385 0.0 6 381 4 212 358 0.0 6 352 6 213 532 0.0 6 520 11 0 0 1 214 464 0.0 6 452 12 215 294 0.0 6 283 11 216 438 0.0 6 429 9 217 504 0.0 6 494 10 218 310 0.0 6 298 11 1 219 268 0.0 6 263 5 220 338 0.0 6 327 11 221 270 0.0 6 262 8 222 426 0.0 6 419 7 223 222 0.0 6 221 1 224 327 0.0 6 319 7 0 1 225 419 0.0 6 408 10 1 226 907 0.0 6 879 28 227 655 0.0 6 634 20 1 228 562 0.0 6 551 11 229 556 0.0 6 542 14 230 510 0.0 6 503 5 2 231 401 0.0 6 390 11 232 498 0.0 6 483 14 1 233 548 0.0 6 536 11 1 234 465 0.0 6 453 12 235 358 0.0 6 354 4 236 289 0.0 6 281 7 1 237 248 0.0 6 241 7 238 373 0.0 6 363 10 239 385 0.0 6 374 11 240 555 0.0 6 536 17 2 241 674 0.0 6 658 16 242 641 0.0 6 624 17 243 655 0.0 6 643 11 0 0 0 0 1 244 422 0.0 6 411 10 1 245 653 0.0 6 645 7 1 246 293 0.0 6 287 6 247 499 0.0 6 483 14 1 1 248 248 0.0 6 236 11 1 249 687 0.0 6 672 14 1 250 448 0.0 6 443 4 1 251 527 0.0 6 506 21 252 701 0.0 6 685 16 253 356 0.0 6 337 18 1 254 756 0.0 6 741 15 255 551 0.0 6 540 11 256 541 0.0 6 522 18 1 257 761 0.0 6 749 12 258 843 0.0 6 800 41 2 259 1160 0.0 6 1126 32 2 260 858 0.0 6 834 23 0 0 1 261 618 0.0 6 606 12 262 789 0.0 6 762 27 263 1491 0.0 6 1461 28 2 264 725 0.0 6 699 24 1 0 0 0 1 265 976 0.0 6 956 18 2 266 601 0.0 6 589 11 1 267 971 0.0 6 947 24 268 913 0.0 6 892 21 269 685 0.0 6 675 10 270 458 0.0 6 447 11 271 771 0.0 6 745 26 272 1957 0.0 6 1918 39 273 968 0.0 6 940 27 1 274 1197 0.0 6 1170 24 3 275 1226 0.0 6 1195 30 1 276 2794 0.0 6 2710 82 2 277 6351 0.0 6 6225 124 2 278 6654 0.0 6 6402 234 17 1 279 263 0.0 6 243 19 1 280 30 0.0 6 25 3 1 1 281 2 0.0 6 2 282 1 0.0 6 0 0 0 0 0 1 283 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 255 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 64 2 0.0 6 2 73 1 0.0 6 1 74 4 0.0 6 4 75 2 0.0 6 2 76 3 0.0 6 3 77 1 0.0 6 1 78 3 0.0 6 3 79 3 0.0 6 3 80 3 0.0 6 3 81 1 0.0 6 1 82 1 0.0 6 1 83 2 0.0 6 2 86 2 0.0 6 2 88 1 0.0 6 1 89 2 0.0 6 2 90 2 0.0 6 2 94 3 0.0 6 3 97 2 0.0 6 2 98 1 0.0 6 1 105 1 0.0 6 1 107 2 0.0 6 2 108 4 0.0 6 4 109 3 0.0 6 3 110 3 0.0 6 3 111 1 0.0 6 1 113 1 0.0 6 1 114 1 0.0 6 1 119 1 0.0 6 1 120 1 0.0 6 1 121 2 0.0 6 2 122 4 0.0 6 4 123 3 0.0 6 3 124 5 0.0 6 5 126 1 0.0 6 1 129 1 0.0 6 1 130 2 0.0 6 2 131 3 0.0 6 3 132 2 0.0 6 2 134 1 0.0 6 1 135 1 0.0 6 1 142 3 0.0 6 3 144 2 0.0 6 2 145 3 0.0 6 3 146 9 0.0 6 9 147 1 0.0 6 1 150 2 0.0 6 2 151 1 0.0 6 1 152 2 0.0 6 2 153 5 0.0 6 5 154 2 0.0 6 2 157 1 0.0 6 1 158 1 0.0 6 1 159 1 0.0 6 1 160 3 0.0 6 3 162 1 0.0 6 1 163 4 0.0 6 4 167 2 0.0 6 2 168 1 0.0 6 1 171 1 0.0 6 1 172 1 0.0 6 1 174 3 0.0 6 3 175 8 0.0 6 8 176 1 0.0 6 1 177 2 0.0 6 2 178 3 0.0 6 3 179 4 0.0 6 4 181 1 0.0 6 1 182 6 0.0 6 6 183 12 0.0 6 12 184 4 0.0 6 4 186 4 0.0 6 4 187 1 0.0 6 1 188 1 0.0 6 1 189 5 0.0 6 5 190 5 0.0 6 5 191 2 0.0 6 2 192 2 0.0 6 2 193 5 0.0 6 5 194 3 0.0 6 3 195 1 0.0 6 1 197 2 0.0 6 2 201 3 0.0 6 3 202 3 0.0 6 3 204 1 0.0 6 1 209 2 0.0 6 2 211 2 0.0 6 2 212 2 0.0 6 2 215 1 0.0 6 1 216 1 0.0 6 1 220 1 0.0 6 1 221 1 0.0 6 1 222 1 0.0 6 1 224 3 0.0 6 3 225 1 0.0 6 1 230 1 0.0 6 1 231 1 0.0 6 1 233 4 0.0 6 4 234 2 0.0 6 2 236 1 0.0 6 1 239 2 0.0 6 2 240 2 0.0 6 2 241 2 0.0 6 2 243 1 0.0 6 1 246 1 0.0 6 1 252 3 0.0 6 3 256 1 0.0 6 1 258 1 0.0 6 1 266 2 0.0 6 2 269 1 0.0 6 1 270 1 0.0 6 1 273 1 0.0 6 1 274 1 0.0 6 1 292 1 0.0 6 1 303 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 66582 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 260.9 1 0 1 5 1 65.2 1 0 1 7 1 4.1 2 1 8 1 1.0 2 1 11 3 0.0 3 2 1 12 2 0.0 3 2 13 6 0.0 3 5 1 14 4 0.0 4 2 2 15 2 0.0 4 2 16 2 0.0 4 2 17 15 0.0 5 13 2 18 2 0.0 5 0 2 19 25 0.0 5 21 3 1 20 64 0.0 6 62 2 21 30 0.0 6 28 2 22 32 0.0 6 30 2 23 25 0.0 6 23 2 24 19 0.0 6 19 25 5 0.0 6 5 26 11 0.0 6 10 1 27 9 0.0 6 8 1 28 11 0.0 6 10 1 29 6 0.0 6 5 0 1 30 12 0.0 6 12 31 14 0.0 6 13 1 32 13 0.0 6 12 1 33 12 0.0 6 10 2 34 13 0.0 6 13 35 5 0.0 6 5 36 11 0.0 6 10 1 37 5 0.0 6 3 2 38 10 0.0 6 10 39 16 0.0 6 15 1 40 11 0.0 6 11 41 6 0.0 6 5 0 1 42 16 0.0 6 15 1 43 5 0.0 6 5 44 17 0.0 6 14 2 0 1 45 11 0.0 6 11 46 18 0.0 6 18 47 9 0.0 6 9 48 12 0.0 6 10 1 1 49 28 0.0 6 26 1 1 50 11 0.0 6 7 3 1 51 21 0.0 6 18 2 0 0 0 1 52 8 0.0 6 7 1 53 8 0.0 6 8 54 7 0.0 6 6 0 1 55 20 0.0 6 14 6 56 20 0.0 6 18 2 57 18 0.0 6 16 1 1 58 15 0.0 6 13 2 59 17 0.0 6 16 1 60 25 0.0 6 22 2 1 61 14 0.0 6 12 2 62 15 0.0 6 14 1 63 10 0.0 6 10 64 12 0.0 6 11 1 65 14 0.0 6 12 2 66 19 0.0 6 17 1 0 0 1 67 16 0.0 6 16 68 19 0.0 6 16 2 0 0 1 69 31 0.0 6 29 2 70 119 0.0 6 109 9 0 1 71 28 0.0 6 28 72 73 0.0 6 68 5 73 17 0.0 6 17 74 29 0.0 6 28 0 1 75 13 0.0 6 12 1 76 18 0.0 6 18 77 13 0.0 6 12 1 78 23 0.0 6 20 3 79 12 0.0 6 12 80 16 0.0 6 15 0 1 81 9 0.0 6 9 82 27 0.0 6 26 1 83 33 0.0 6 31 1 1 84 46 0.0 6 41 4 0 0 0 1 85 45 0.0 6 40 5 86 38 0.0 6 37 1 87 57 0.0 6 48 8 1 88 12 0.0 6 12 89 25 0.0 6 24 1 90 50 0.0 6 47 3 91 28 0.0 6 25 2 0 0 1 92 24 0.0 6 23 0 0 1 93 28 0.0 6 27 1 94 68 0.0 6 66 2 95 27 0.0 6 26 1 96 46 0.0 6 39 5 1 0 1 97 30 0.0 6 29 0 1 98 22 0.0 6 20 2 99 46 0.0 6 40 5 0 1 100 33 0.0 6 30 3 101 31 0.0 6 29 2 102 54 0.0 6 49 5 103 69 0.0 6 65 4 104 73 0.0 6 68 5 105 63 0.0 6 60 2 1 106 46 0.0 6 42 3 1 107 88 0.0 6 82 5 1 108 51 0.0 6 45 6 109 40 0.0 6 40 110 52 0.0 6 49 1 0 0 2 111 45 0.0 6 42 2 0 0 1 112 50 0.0 6 49 0 1 113 51 0.0 6 49 1 0 0 1 114 50 0.0 6 46 4 115 56 0.0 6 54 2 116 44 0.0 6 43 1 117 53 0.0 6 48 5 118 62 0.0 6 55 7 119 53 0.0 6 52 1 120 109 0.0 6 104 4 1 121 75 0.0 6 73 2 122 87 0.0 6 83 3 0 0 1 123 91 0.0 6 85 6 124 118 0.0 6 111 6 0 0 0 1 125 88 0.0 6 88 126 55 0.0 6 52 3 127 91 0.0 6 88 3 128 74 0.0 6 72 2 129 75 0.0 6 75 130 84 0.0 6 80 4 131 114 0.0 6 106 6 1 1 132 168 0.0 6 163 5 133 138 0.0 6 131 7 134 198 0.0 6 189 8 0 1 135 282 0.0 6 270 11 1 136 165 0.0 6 149 13 2 0 1 137 254 0.0 6 248 6 138 161 0.0 6 157 3 0 0 0 1 139 179 0.0 6 170 9 140 208 0.0 6 200 7 0 0 0 0 1 141 276 0.0 6 267 8 1 142 325 0.0 6 312 13 143 341 0.0 6 330 9 0 0 2 144 487 0.0 6 477 9 0 1 145 273 0.0 6 266 7 146 260 0.0 6 253 6 1 147 235 0.0 6 228 6 1 148 259 0.0 6 250 7 0 0 2 149 399 0.0 6 379 17 1 0 1 1 150 444 0.0 6 429 13 2 151 371 0.0 6 363 8 152 586 0.0 6 562 20 3 0 1 153 578 0.0 6 561 16 1 154 489 0.0 6 474 15 155 521 0.0 6 503 17 0 0 0 1 156 475 0.0 6 454 19 1 1 157 520 0.0 6 497 23 158 546 0.0 6 533 11 1 0 1 159 295 0.0 6 279 16 160 623 0.0 6 603 18 1 0 0 1 161 405 0.0 6 391 12 0 0 2 162 382 0.0 6 371 11 163 517 0.0 6 503 13 1 164 464 0.0 6 450 13 1 165 513 0.0 6 492 16 4 1 166 725 0.0 6 698 22 3 0 2 167 371 0.0 6 357 12 2 168 801 0.0 6 768 31 0 2 169 310 0.0 6 296 14 170 336 0.0 6 319 14 1 1 1 171 639 0.0 6 618 19 1 1 172 340 0.0 6 326 14 173 327 0.0 6 320 5 1 0 0 1 174 469 0.0 6 451 18 175 835 0.0 6 818 16 1 176 549 0.0 6 531 18 177 841 0.0 6 807 30 4 178 561 0.0 6 534 25 2 179 663 0.0 6 647 14 0 0 2 180 638 0.0 6 615 22 0 1 181 978 0.0 6 952 24 1 0 0 0 1 182 394 0.0 6 373 20 1 183 758 0.0 6 725 31 0 0 1 1 184 285 0.0 6 276 6 3 185 304 0.0 6 291 12 1 186 889 0.0 6 866 21 2 187 1137 0.0 6 1092 41 2 1 1 188 1454 0.0 6 1405 46 1 1 0 1 189 1304 0.0 6 1257 45 1 0 1 190 1666 0.0 6 1612 53 1 191 1444 0.0 6 1396 46 2 192 1158 0.0 6 1120 37 1 193 845 0.0 6 814 30 0 1 194 1039 0.0 6 1006 30 2 0 1 195 1029 0.0 6 991 38 196 3145 0.0 6 3065 75 2 0 2 1 197 641 0.0 6 616 23 2 198 1362 0.0 6 1293 69 199 3191 0.0 6 3100 83 4 0 3 1 200 825 0.0 6 802 21 2 201 1228 0.0 6 1185 42 0 1 202 769 0.0 6 752 16 0 1 203 554 0.0 6 541 12 1 204 711 0.0 6 690 17 3 0 1 205 489 0.0 6 480 8 1 206 430 0.0 6 416 14 207 347 0.0 6 335 11 1 208 692 0.0 6 666 24 1 0 1 209 338 0.0 6 329 9 210 435 0.0 6 420 15 211 686 0.0 6 648 36 1 0 1 212 665 0.0 6 642 23 213 141 0.0 6 138 3 214 529 0.0 6 512 16 0 0 1 215 340 0.0 6 330 8 1 0 1 216 649 0.0 6 628 21 217 641 0.0 6 621 19 0 0 1 218 301 0.0 6 288 12 1 219 289 0.0 6 277 11 0 0 1 220 229 0.0 6 223 6 221 316 0.0 6 304 12 222 664 0.0 6 652 12 223 579 0.0 6 566 11 0 0 2 224 119 0.0 6 113 6 225 291 0.0 6 279 10 0 0 2 226 288 0.0 6 277 11 227 519 0.0 6 504 13 2 228 287 0.0 6 275 11 1 229 373 0.0 6 358 15 230 544 0.0 6 522 22 231 338 0.0 6 325 13 232 717 0.0 6 698 19 233 189 0.0 6 180 9 234 210 0.0 6 203 7 235 173 0.0 6 168 5 236 109 0.0 6 104 4 1 237 176 0.0 6 171 5 238 75 0.0 6 70 5 239 28 0.0 6 28 240 62 0.0 6 58 4 241 115 0.0 6 111 4 242 137 0.0 6 134 2 0 1 243 77 0.0 6 72 5 244 179 0.0 6 171 6 2 245 169 0.0 6 165 4 246 79 0.0 6 78 1 247 58 0.0 6 56 2 248 49 0.0 6 48 1 249 68 0.0 6 68 250 62 0.0 6 58 4 251 137 0.0 6 133 4 252 48 0.0 6 47 1 253 20 0.0 6 20 254 144 0.0 6 141 3 255 18 0.0 6 18 256 17 0.0 6 15 2 257 3 0.0 6 3 258 1 0.0 6 1 260 3 0.0 6 3 265 1 0.0 6 1 268 1 0.0 6 1 270 1 0.0 6 0 1 280 6 0.0 6 5 1 281 17 0.0 6 16 1 282 74 0.0 6 71 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 176 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 2 0.0 6 2 70 1 0.0 6 1 71 7 0.0 6 7 72 4 0.0 6 4 75 2 0.0 6 2 76 4 0.0 6 4 78 2 0.0 6 2 79 1 0.0 6 1 80 1 0.0 6 1 81 1 0.0 6 1 84 2 0.0 6 2 85 2 0.0 6 2 87 1 0.0 6 1 88 3 0.0 6 3 89 1 0.0 6 1 90 1 0.0 6 1 91 1 0.0 6 1 92 2 0.0 6 2 93 1 0.0 6 1 94 1 0.0 6 1 95 2 0.0 6 2 97 1 0.0 6 1 100 1 0.0 6 1 101 1 0.0 6 1 103 2 0.0 6 2 104 3 0.0 6 3 107 2 0.0 6 2 108 1 0.0 6 1 109 1 0.0 6 1 111 2 0.0 6 2 112 1 0.0 6 1 114 2 0.0 6 2 117 1 0.0 6 1 118 8 0.0 6 8 119 3 0.0 6 3 120 2 0.0 6 2 121 1 0.0 6 1 122 1 0.0 6 1 123 1 0.0 6 1 126 1 0.0 6 1 127 1 0.0 6 1 137 1 0.0 6 1 139 1 0.0 6 1 141 4 0.0 6 4 142 12 0.0 6 12 143 2 0.0 6 2 144 1 0.0 6 1 147 2 0.0 6 2 148 5 0.0 6 5 149 4 0.0 6 4 153 2 0.0 6 2 155 1 0.0 6 1 158 2 0.0 6 2 159 1 0.0 6 1 161 1 0.0 6 1 162 1 0.0 6 1 163 1 0.0 6 1 168 2 0.0 6 2 171 5 0.0 6 5 173 7 0.0 6 7 174 2 0.0 6 2 175 4 0.0 6 4 177 2 0.0 6 2 178 2 0.0 6 2 179 8 0.0 6 8 182 1 0.0 6 1 183 1 0.0 6 1 187 3 0.0 6 2 0 0 0 1 189 1 0.0 6 1 190 5 0.0 6 5 193 3 0.0 6 3 198 1 0.0 6 1 199 2 0.0 6 2 205 2 0.0 6 2 208 1 0.0 6 1 210 1 0.0 6 1 211 2 0.0 6 2 212 1 0.0 6 1 229 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_15_78_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_15_305_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_15_78_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_15_305_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.21 s (19 µs/read; 3.23 M reads/minute). === Summary === Total read pairs processed: 119,195 Read 1 with adapter: 119,178 (100.0%) Read 2 with adapter: 119,135 (99.9%) == Read fate breakdown == Pairs that were too short: 19 (0.0%) Pairs discarded as untrimmed: 77 (0.1%) Pairs written (passing filters): 119,099 (99.9%) Total basepairs processed: 47,242,667 bp Read 1: 24,948,967 bp Read 2: 22,293,700 bp Total written (filtered): 8,334,375 bp (17.6%) Read 1: 4,251,007 bp Read 2: 4,083,368 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 114100 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 1862.4 0 1 3 4 10 465.6 1 3 7 6 4 29.1 1 2 0 2 7 4 7.3 2 2 0 2 9 6 0.5 2 6 10 2 0.1 3 2 12 1 0.0 3 1 13 2 0.0 3 2 14 2 0.0 4 2 15 2 0.0 4 1 1 16 6 0.0 4 5 1 17 5 0.0 5 3 0 1 1 18 2 0.0 5 2 19 10 0.0 5 10 20 264 0.0 6 255 7 2 21 106 0.0 6 100 6 22 136 0.0 6 133 3 23 60 0.0 6 59 1 24 51 0.0 6 45 6 25 52 0.0 6 51 1 26 48 0.0 6 43 4 0 0 0 1 27 27 0.0 6 25 2 28 23 0.0 6 21 1 1 29 17 0.0 6 17 30 18 0.0 6 18 31 14 0.0 6 14 32 13 0.0 6 11 2 33 15 0.0 6 14 1 34 18 0.0 6 17 1 35 16 0.0 6 13 3 36 12 0.0 6 11 1 37 8 0.0 6 7 1 38 8 0.0 6 8 39 15 0.0 6 14 1 40 11 0.0 6 11 41 8 0.0 6 6 0 0 0 1 0 1 42 10 0.0 6 10 43 6 0.0 6 6 44 8 0.0 6 8 45 8 0.0 6 8 46 13 0.0 6 13 47 12 0.0 6 11 1 48 8 0.0 6 7 1 49 16 0.0 6 15 0 1 50 13 0.0 6 13 51 13 0.0 6 12 1 52 7 0.0 6 7 53 15 0.0 6 12 3 54 12 0.0 6 12 55 11 0.0 6 9 2 56 4 0.0 6 4 57 5 0.0 6 4 0 0 0 1 58 10 0.0 6 10 59 6 0.0 6 6 60 8 0.0 6 8 61 7 0.0 6 7 62 10 0.0 6 10 63 7 0.0 6 7 64 11 0.0 6 11 65 11 0.0 6 11 66 14 0.0 6 14 67 7 0.0 6 7 68 11 0.0 6 9 2 69 14 0.0 6 14 70 14 0.0 6 12 2 71 16 0.0 6 15 1 72 6 0.0 6 6 73 13 0.0 6 12 1 74 21 0.0 6 19 2 75 17 0.0 6 17 76 10 0.0 6 8 0 1 0 1 77 13 0.0 6 12 1 78 12 0.0 6 12 79 16 0.0 6 16 80 18 0.0 6 18 81 31 0.0 6 31 82 26 0.0 6 25 1 83 33 0.0 6 33 84 20 0.0 6 19 1 85 19 0.0 6 19 86 15 0.0 6 15 87 16 0.0 6 16 88 15 0.0 6 14 1 89 12 0.0 6 12 90 12 0.0 6 12 91 13 0.0 6 13 92 20 0.0 6 20 93 19 0.0 6 19 94 20 0.0 6 20 95 18 0.0 6 16 2 96 21 0.0 6 21 97 16 0.0 6 16 98 12 0.0 6 10 2 99 17 0.0 6 17 100 25 0.0 6 23 2 101 31 0.0 6 31 102 34 0.0 6 33 1 103 27 0.0 6 26 1 104 44 0.0 6 41 2 1 105 21 0.0 6 20 1 106 35 0.0 6 33 2 107 33 0.0 6 33 108 33 0.0 6 33 109 43 0.0 6 41 2 110 23 0.0 6 23 111 66 0.0 6 66 112 52 0.0 6 52 113 57 0.0 6 56 0 0 1 114 60 0.0 6 60 115 69 0.0 6 69 116 61 0.0 6 61 117 62 0.0 6 62 118 61 0.0 6 61 119 63 0.0 6 63 120 80 0.0 6 80 121 54 0.0 6 53 1 122 58 0.0 6 58 123 55 0.0 6 53 2 124 70 0.0 6 70 125 69 0.0 6 68 1 126 75 0.0 6 74 0 1 127 78 0.0 6 78 128 86 0.0 6 86 129 99 0.0 6 99 130 105 0.0 6 105 131 92 0.0 6 90 2 132 101 0.0 6 100 1 133 119 0.0 6 119 134 97 0.0 6 96 1 135 141 0.0 6 138 3 136 135 0.0 6 130 5 137 382 0.0 6 377 5 138 204 0.0 6 202 2 139 169 0.0 6 168 0 0 0 0 0 1 140 163 0.0 6 161 2 141 185 0.0 6 184 0 0 0 0 1 142 274 0.0 6 273 1 143 752 0.0 6 748 4 144 1168 0.0 6 1166 1 0 1 145 1132 0.0 6 1131 1 146 1667 0.0 6 1664 3 147 2210 0.0 6 2208 2 148 2690 0.0 6 2687 3 149 2734 0.0 6 2731 3 150 2769 0.0 6 2760 9 151 2892 0.0 6 2884 5 0 2 0 1 152 2533 0.0 6 2523 10 153 3303 0.0 6 3294 9 154 1828 0.0 6 1827 1 155 1916 0.0 6 1911 5 156 1588 0.0 6 1588 157 1967 0.0 6 1964 3 158 1280 0.0 6 1279 1 159 1779 0.0 6 1777 2 160 1798 0.0 6 1796 2 161 1591 0.0 6 1587 4 162 1592 0.0 6 1589 1 0 1 1 163 1613 0.0 6 1606 6 0 0 0 1 164 1786 0.0 6 1784 2 165 1273 0.0 6 1270 3 166 1509 0.0 6 1508 1 167 1002 0.0 6 1001 1 168 1182 0.0 6 1181 1 169 1385 0.0 6 1382 2 0 0 0 0 1 170 1162 0.0 6 1161 1 171 1328 0.0 6 1324 3 0 1 172 1345 0.0 6 1345 173 962 0.0 6 958 4 174 1358 0.0 6 1355 3 175 1260 0.0 6 1258 2 176 1626 0.0 6 1623 3 177 1272 0.0 6 1268 4 178 1302 0.0 6 1299 3 179 726 0.0 6 725 0 0 0 0 1 180 759 0.0 6 759 181 1122 0.0 6 1121 1 182 1469 0.0 6 1468 1 183 1159 0.0 6 1152 6 0 0 0 0 1 184 1159 0.0 6 1156 3 185 1110 0.0 6 1108 2 186 792 0.0 6 790 2 187 733 0.0 6 733 188 691 0.0 6 688 2 0 1 189 530 0.0 6 528 2 190 453 0.0 6 452 1 191 675 0.0 6 674 1 192 779 0.0 6 779 193 622 0.0 6 620 2 194 1201 0.0 6 1199 1 0 0 0 1 195 1026 0.0 6 1026 196 1165 0.0 6 1164 1 197 1476 0.0 6 1476 198 974 0.0 6 973 1 199 941 0.0 6 940 1 200 871 0.0 6 871 201 708 0.0 6 708 202 943 0.0 6 942 1 203 790 0.0 6 788 2 204 759 0.0 6 758 1 205 1324 0.0 6 1323 1 206 944 0.0 6 943 1 207 1193 0.0 6 1191 2 208 870 0.0 6 870 209 597 0.0 6 597 210 816 0.0 6 816 211 844 0.0 6 841 2 0 0 1 212 758 0.0 6 758 213 888 0.0 6 888 214 779 0.0 6 778 1 215 596 0.0 6 596 216 663 0.0 6 663 217 692 0.0 6 692 218 515 0.0 6 514 1 219 475 0.0 6 475 220 520 0.0 6 519 1 221 446 0.0 6 445 1 222 426 0.0 6 425 1 223 500 0.0 6 499 1 224 628 0.0 6 626 1 0 0 0 0 1 225 562 0.0 6 562 226 421 0.0 6 421 227 419 0.0 6 419 228 395 0.0 6 395 229 434 0.0 6 434 230 428 0.0 6 428 231 449 0.0 6 448 0 0 0 0 1 232 408 0.0 6 408 233 375 0.0 6 373 2 234 319 0.0 6 319 235 386 0.0 6 385 1 236 321 0.0 6 320 1 237 279 0.0 6 279 238 357 0.0 6 357 239 368 0.0 6 367 1 240 238 0.0 6 237 1 241 324 0.0 6 324 242 233 0.0 6 233 243 181 0.0 6 181 244 192 0.0 6 191 1 245 201 0.0 6 201 246 155 0.0 6 155 247 193 0.0 6 193 248 149 0.0 6 149 249 122 0.0 6 122 250 103 0.0 6 103 251 87 0.0 6 87 252 97 0.0 6 97 253 85 0.0 6 85 254 63 0.0 6 63 255 49 0.0 6 49 256 58 0.0 6 58 257 52 0.0 6 52 258 55 0.0 6 55 259 44 0.0 6 44 260 40 0.0 6 40 261 44 0.0 6 44 262 22 0.0 6 22 263 30 0.0 6 30 264 19 0.0 6 19 265 21 0.0 6 21 266 33 0.0 6 33 267 21 0.0 6 21 268 37 0.0 6 37 269 16 0.0 6 16 270 12 0.0 6 12 271 23 0.0 6 23 272 19 0.0 6 19 273 16 0.0 6 16 274 25 0.0 6 25 275 38 0.0 6 38 276 61 0.0 6 61 277 141 0.0 6 140 1 278 633 0.0 6 632 1 279 6 0.0 6 6 280 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 5078 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 9 1 0.5 2 0 0 0 1 60 1 0.0 6 0 0 0 0 1 61 1 0.0 6 1 62 2 0.0 6 2 63 19 0.0 6 14 4 1 64 647 0.0 6 617 29 1 65 3 0.0 6 3 66 10 0.0 6 8 2 71 4 0.0 6 3 0 0 0 0 0 1 72 35 0.0 6 34 1 73 37 0.0 6 37 74 40 0.0 6 40 75 42 0.0 6 42 76 98 0.0 6 98 77 104 0.0 6 104 78 124 0.0 6 122 2 79 131 0.0 6 130 1 80 124 0.0 6 123 1 81 132 0.0 6 132 82 131 0.0 6 131 83 125 0.0 6 125 84 66 0.0 6 65 1 85 80 0.0 6 80 86 80 0.0 6 80 87 74 0.0 6 74 88 100 0.0 6 99 1 89 68 0.0 6 68 90 82 0.0 6 82 91 75 0.0 6 75 92 68 0.0 6 67 1 93 64 0.0 6 64 94 78 0.0 6 76 2 95 65 0.0 6 65 96 63 0.0 6 63 97 37 0.0 6 36 1 98 45 0.0 6 45 99 52 0.0 6 52 100 43 0.0 6 43 101 48 0.0 6 48 102 44 0.0 6 44 103 38 0.0 6 38 104 55 0.0 6 55 105 67 0.0 6 67 106 60 0.0 6 60 107 46 0.0 6 46 108 46 0.0 6 45 1 109 30 0.0 6 30 110 34 0.0 6 34 111 49 0.0 6 49 112 56 0.0 6 56 113 48 0.0 6 48 114 49 0.0 6 49 115 28 0.0 6 28 116 20 0.0 6 20 117 24 0.0 6 24 118 24 0.0 6 23 1 119 13 0.0 6 13 120 8 0.0 6 8 121 26 0.0 6 26 122 22 0.0 6 22 123 20 0.0 6 20 124 33 0.0 6 33 125 27 0.0 6 27 126 29 0.0 6 29 127 38 0.0 6 38 128 28 0.0 6 27 1 129 39 0.0 6 39 130 29 0.0 6 29 131 23 0.0 6 23 132 22 0.0 6 22 133 27 0.0 6 27 134 20 0.0 6 20 135 36 0.0 6 36 136 27 0.0 6 27 137 31 0.0 6 31 138 21 0.0 6 21 139 15 0.0 6 15 140 28 0.0 6 28 141 29 0.0 6 29 142 22 0.0 6 22 143 28 0.0 6 28 144 10 0.0 6 10 145 6 0.0 6 6 146 25 0.0 6 25 147 21 0.0 6 21 148 5 0.0 6 5 149 12 0.0 6 12 150 13 0.0 6 13 151 9 0.0 6 9 152 15 0.0 6 15 153 10 0.0 6 10 154 17 0.0 6 16 1 155 16 0.0 6 16 156 6 0.0 6 6 157 3 0.0 6 3 158 17 0.0 6 17 159 19 0.0 6 19 160 14 0.0 6 14 161 17 0.0 6 17 162 15 0.0 6 15 163 16 0.0 6 16 164 17 0.0 6 17 165 23 0.0 6 23 166 9 0.0 6 9 167 12 0.0 6 12 168 31 0.0 6 31 169 12 0.0 6 12 170 16 0.0 6 16 171 17 0.0 6 17 172 8 0.0 6 8 173 5 0.0 6 5 174 11 0.0 6 11 175 14 0.0 6 14 176 9 0.0 6 8 1 177 19 0.0 6 19 178 12 0.0 6 12 179 7 0.0 6 6 1 180 10 0.0 6 10 181 8 0.0 6 8 182 9 0.0 6 9 183 12 0.0 6 12 184 14 0.0 6 14 185 10 0.0 6 10 186 5 0.0 6 5 187 5 0.0 6 5 188 3 0.0 6 3 189 6 0.0 6 6 190 5 0.0 6 5 191 2 0.0 6 2 192 4 0.0 6 4 193 4 0.0 6 4 194 5 0.0 6 5 195 8 0.0 6 8 196 1 0.0 6 1 197 4 0.0 6 4 198 3 0.0 6 3 199 8 0.0 6 8 200 2 0.0 6 2 201 6 0.0 6 6 202 6 0.0 6 6 203 7 0.0 6 7 204 5 0.0 6 5 206 3 0.0 6 3 207 2 0.0 6 2 208 2 0.0 6 2 209 2 0.0 6 2 210 7 0.0 6 7 211 3 0.0 6 3 212 4 0.0 6 4 213 4 0.0 6 4 214 4 0.0 6 4 215 4 0.0 6 4 216 3 0.0 6 3 217 1 0.0 6 1 218 3 0.0 6 3 219 1 0.0 6 1 220 3 0.0 6 3 221 4 0.0 6 4 222 1 0.0 6 1 224 2 0.0 6 2 225 3 0.0 6 3 226 2 0.0 6 2 227 3 0.0 6 3 228 2 0.0 6 2 231 1 0.0 6 1 232 1 0.0 6 1 233 1 0.0 6 1 234 1 0.0 6 1 235 2 0.0 6 2 237 1 0.0 6 1 238 1 0.0 6 1 239 1 0.0 6 1 240 1 0.0 6 1 242 1 0.0 6 1 245 1 0.0 6 1 249 1 0.0 6 1 253 1 0.0 6 1 260 1 0.0 6 1 261 1 0.0 6 1 289 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 113823 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1862.4 0 0 2 4 1 465.6 1 1 7 1 7.3 2 1 10 6 0.1 3 5 1 11 2 0.0 3 2 12 3 0.0 3 2 1 13 10 0.0 3 8 1 1 14 9 0.0 4 9 15 4 0.0 4 3 1 16 15 0.0 4 14 1 17 17 0.0 5 16 1 18 18 0.0 5 15 3 19 18 0.0 5 18 20 159 0.0 6 154 5 21 69 0.0 6 66 3 22 39 0.0 6 38 1 23 41 0.0 6 37 3 1 24 28 0.0 6 28 25 26 0.0 6 26 26 27 0.0 6 25 1 0 0 0 0 1 27 12 0.0 6 12 28 27 0.0 6 24 1 0 0 0 0 2 29 25 0.0 6 22 2 0 0 0 0 1 30 25 0.0 6 25 31 19 0.0 6 19 32 9 0.0 6 9 33 13 0.0 6 12 1 34 14 0.0 6 14 35 7 0.0 6 7 36 8 0.0 6 8 37 17 0.0 6 16 1 38 13 0.0 6 13 39 19 0.0 6 17 2 40 14 0.0 6 14 41 16 0.0 6 16 42 12 0.0 6 11 1 43 15 0.0 6 15 44 9 0.0 6 8 1 45 22 0.0 6 20 2 46 16 0.0 6 15 1 47 24 0.0 6 22 2 48 23 0.0 6 21 1 0 0 1 49 19 0.0 6 19 50 15 0.0 6 14 1 51 16 0.0 6 15 1 52 20 0.0 6 19 1 53 25 0.0 6 23 2 54 25 0.0 6 22 1 1 1 55 20 0.0 6 20 56 22 0.0 6 21 1 57 13 0.0 6 13 58 29 0.0 6 26 1 1 1 59 20 0.0 6 20 60 29 0.0 6 28 1 61 19 0.0 6 17 1 1 62 29 0.0 6 28 0 1 63 37 0.0 6 32 5 64 21 0.0 6 19 2 65 20 0.0 6 20 66 62 0.0 6 59 3 67 184 0.0 6 178 5 1 68 61 0.0 6 56 4 0 0 1 69 106 0.0 6 105 1 70 44 0.0 6 39 5 71 63 0.0 6 59 2 0 0 1 0 1 72 45 0.0 6 42 1 1 1 73 44 0.0 6 39 4 0 0 0 0 1 74 34 0.0 6 34 75 52 0.0 6 48 3 0 0 0 1 76 51 0.0 6 50 1 77 50 0.0 6 49 1 78 37 0.0 6 34 0 1 0 0 1 1 79 33 0.0 6 32 1 80 55 0.0 6 55 81 44 0.0 6 41 3 82 57 0.0 6 57 83 99 0.0 6 97 2 84 45 0.0 6 44 1 85 54 0.0 6 53 1 86 37 0.0 6 36 1 87 35 0.0 6 34 1 88 54 0.0 6 53 1 89 61 0.0 6 58 1 2 90 71 0.0 6 70 1 91 65 0.0 6 59 5 0 1 92 89 0.0 6 85 3 0 0 1 93 83 0.0 6 79 3 0 1 94 59 0.0 6 57 2 95 84 0.0 6 83 0 0 0 0 0 1 96 108 0.0 6 102 5 0 0 0 0 1 97 86 0.0 6 85 1 98 78 0.0 6 75 2 1 99 69 0.0 6 69 100 91 0.0 6 86 5 101 86 0.0 6 84 2 102 94 0.0 6 93 1 103 113 0.0 6 109 4 104 177 0.0 6 173 3 0 0 1 105 93 0.0 6 93 106 98 0.0 6 98 107 105 0.0 6 104 1 108 97 0.0 6 96 1 109 114 0.0 6 112 1 0 1 110 132 0.0 6 129 3 111 128 0.0 6 126 1 1 112 163 0.0 6 159 4 113 141 0.0 6 140 1 114 144 0.0 6 143 0 0 1 115 170 0.0 6 169 1 116 186 0.0 6 179 6 0 0 0 1 117 202 0.0 6 200 1 0 0 0 1 118 234 0.0 6 230 3 0 0 0 0 1 119 217 0.0 6 212 4 0 0 0 0 1 120 190 0.0 6 188 1 0 0 0 1 121 237 0.0 6 235 2 122 230 0.0 6 229 1 123 265 0.0 6 262 3 124 296 0.0 6 292 3 1 125 347 0.0 6 345 1 0 0 0 1 126 385 0.0 6 382 3 127 388 0.0 6 386 2 128 475 0.0 6 471 3 1 129 510 0.0 6 505 5 130 529 0.0 6 520 8 1 131 583 0.0 6 575 7 0 0 0 0 1 132 995 0.0 6 973 21 1 133 697 0.0 6 687 7 2 0 1 134 699 0.0 6 692 5 1 0 0 0 1 135 625 0.0 6 619 4 0 0 2 136 646 0.0 6 642 3 1 137 585 0.0 6 581 3 0 0 1 138 637 0.0 6 636 1 139 703 0.0 6 698 4 0 0 0 1 140 929 0.0 6 926 3 141 1495 0.0 6 1487 5 1 1 0 0 1 142 1941 0.0 6 1933 8 143 1766 0.0 6 1758 8 144 2404 0.0 6 2397 6 0 0 1 145 2615 0.0 6 2608 5 1 0 1 146 3002 0.0 6 2996 5 1 147 3257 0.0 6 3245 8 1 1 0 2 148 3474 0.0 6 3464 9 1 149 3467 0.0 6 3458 6 0 1 0 1 1 150 4240 0.0 6 4230 8 0 1 1 151 3584 0.0 6 3583 1 152 2905 0.0 6 2900 5 153 2993 0.0 6 2987 4 0 1 0 0 1 154 2291 0.0 6 2287 4 155 2657 0.0 6 2647 9 0 0 0 0 1 156 2053 0.0 6 2048 3 0 2 157 2449 0.0 6 2441 8 158 2387 0.0 6 2384 3 159 1988 0.0 6 1978 8 0 0 0 1 1 160 1999 0.0 6 1989 8 1 0 0 0 1 161 1794 0.0 6 1791 3 162 1718 0.0 6 1712 6 163 1650 0.0 6 1646 3 0 0 1 164 1535 0.0 6 1529 4 1 0 1 165 1434 0.0 6 1429 4 0 0 0 0 1 166 1738 0.0 6 1736 0 0 1 0 1 167 1519 0.0 6 1516 2 0 0 1 168 1826 0.0 6 1824 2 169 1566 0.0 6 1564 2 170 1497 0.0 6 1492 5 171 1479 0.0 6 1475 4 172 1647 0.0 6 1643 4 173 1698 0.0 6 1693 5 174 1677 0.0 6 1675 2 175 1471 0.0 6 1470 1 176 1495 0.0 6 1495 177 1132 0.0 6 1127 4 0 0 1 178 1201 0.0 6 1198 2 0 0 0 0 1 179 943 0.0 6 941 2 180 853 0.0 6 852 1 181 735 0.0 6 735 182 751 0.0 6 751 183 968 0.0 6 967 1 184 1255 0.0 6 1252 2 0 0 0 0 1 185 1099 0.0 6 1098 0 0 0 1 186 754 0.0 6 752 1 0 0 0 1 187 768 0.0 6 766 1 0 0 1 188 685 0.0 6 685 189 658 0.0 6 657 1 190 550 0.0 6 549 1 191 537 0.0 6 536 1 192 450 0.0 6 450 193 380 0.0 6 379 0 0 1 194 379 0.0 6 377 2 195 334 0.0 6 334 196 387 0.0 6 387 197 330 0.0 6 330 198 267 0.0 6 266 1 199 249 0.0 6 248 1 200 223 0.0 6 223 201 169 0.0 6 169 202 169 0.0 6 169 203 160 0.0 6 160 204 158 0.0 6 158 205 156 0.0 6 155 0 0 0 0 1 206 129 0.0 6 129 207 132 0.0 6 132 208 96 0.0 6 96 209 100 0.0 6 100 210 66 0.0 6 66 211 73 0.0 6 73 212 79 0.0 6 78 0 0 0 0 0 1 213 72 0.0 6 72 214 66 0.0 6 66 215 57 0.0 6 57 216 44 0.0 6 44 217 38 0.0 6 38 218 31 0.0 6 31 219 19 0.0 6 19 220 24 0.0 6 24 221 22 0.0 6 22 222 32 0.0 6 32 223 18 0.0 6 18 224 15 0.0 6 15 225 18 0.0 6 18 226 13 0.0 6 13 227 8 0.0 6 8 228 7 0.0 6 7 229 2 0.0 6 2 230 4 0.0 6 4 231 5 0.0 6 5 232 2 0.0 6 2 233 2 0.0 6 2 234 2 0.0 6 2 235 4 0.0 6 4 236 5 0.0 6 5 239 4 0.0 6 4 240 3 0.0 6 3 241 1 0.0 6 1 242 2 0.0 6 2 243 1 0.0 6 1 245 2 0.0 6 2 246 1 0.0 6 1 275 2 0.0 6 1 0 0 0 0 1 277 2 0.0 6 1 0 1 278 9 0.0 6 9 279 29 0.0 6 28 1 280 3 0.0 6 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 5312 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 52 2 0.0 6 2 57 8 0.0 6 8 59 1 0.0 6 1 62 1 0.0 6 1 63 8 0.0 6 8 64 62 0.0 6 60 2 65 14 0.0 6 9 5 66 387 0.0 6 380 7 67 1 0.0 6 0 0 0 1 68 3 0.0 6 3 70 2 0.0 6 1 0 0 0 1 71 34 0.0 6 33 1 72 52 0.0 6 52 73 47 0.0 6 46 0 0 1 74 53 0.0 6 53 75 92 0.0 6 92 76 112 0.0 6 112 77 106 0.0 6 105 1 78 200 0.0 6 199 0 1 79 147 0.0 6 146 0 0 0 1 80 146 0.0 6 146 81 187 0.0 6 187 82 168 0.0 6 163 4 0 1 83 72 0.0 6 70 1 0 0 1 84 104 0.0 6 103 0 0 1 85 69 0.0 6 65 0 0 0 4 86 109 0.0 6 108 0 0 0 1 87 65 0.0 6 65 88 79 0.0 6 78 0 0 0 1 89 99 0.0 6 99 90 74 0.0 6 73 1 91 73 0.0 6 73 92 69 0.0 6 69 93 85 0.0 6 84 1 94 65 0.0 6 63 0 0 0 2 95 63 0.0 6 63 96 37 0.0 6 37 97 55 0.0 6 55 98 75 0.0 6 75 99 65 0.0 6 63 1 0 0 1 100 105 0.0 6 103 1 0 0 1 101 43 0.0 6 43 102 40 0.0 6 38 0 0 0 2 103 54 0.0 6 54 104 47 0.0 6 47 105 75 0.0 6 75 106 74 0.0 6 72 1 0 0 1 107 83 0.0 6 83 108 39 0.0 6 38 1 109 42 0.0 6 41 0 0 0 1 110 55 0.0 6 53 0 0 0 2 111 52 0.0 6 52 112 49 0.0 6 49 113 39 0.0 6 39 114 39 0.0 6 38 0 0 0 1 115 161 0.0 6 159 1 0 1 116 26 0.0 6 23 3 117 31 0.0 6 30 0 0 0 1 118 11 0.0 6 9 1 0 1 119 14 0.0 6 14 120 13 0.0 6 13 121 21 0.0 6 19 0 0 0 2 122 19 0.0 6 19 123 38 0.0 6 38 124 19 0.0 6 19 125 32 0.0 6 32 126 35 0.0 6 35 127 20 0.0 6 20 128 36 0.0 6 36 129 29 0.0 6 29 130 26 0.0 6 26 131 15 0.0 6 15 132 21 0.0 6 20 1 133 22 0.0 6 22 134 19 0.0 6 19 135 23 0.0 6 23 136 36 0.0 6 36 137 26 0.0 6 26 138 16 0.0 6 16 139 34 0.0 6 34 140 27 0.0 6 27 141 19 0.0 6 18 1 142 32 0.0 6 31 0 0 0 0 0 1 143 14 0.0 6 14 144 8 0.0 6 8 145 19 0.0 6 19 146 19 0.0 6 19 147 9 0.0 6 9 148 9 0.0 6 9 149 9 0.0 6 9 150 9 0.0 6 9 151 5 0.0 6 5 152 13 0.0 6 13 153 36 0.0 6 36 154 13 0.0 6 13 155 8 0.0 6 8 156 10 0.0 6 9 1 157 11 0.0 6 11 158 14 0.0 6 14 159 18 0.0 6 18 160 7 0.0 6 7 161 18 0.0 6 18 162 9 0.0 6 9 163 29 0.0 6 29 164 22 0.0 6 22 165 8 0.0 6 8 166 11 0.0 6 11 167 13 0.0 6 13 168 18 0.0 6 18 169 15 0.0 6 15 170 14 0.0 6 14 171 9 0.0 6 9 172 2 0.0 6 2 173 7 0.0 6 7 174 17 0.0 6 17 175 6 0.0 6 6 176 12 0.0 6 12 177 10 0.0 6 10 178 12 0.0 6 12 179 7 0.0 6 7 180 10 0.0 6 10 181 7 0.0 6 7 182 13 0.0 6 13 183 8 0.0 6 8 184 2 0.0 6 2 185 3 0.0 6 3 186 4 0.0 6 4 187 4 0.0 6 4 188 6 0.0 6 6 189 9 0.0 6 9 191 1 0.0 6 1 192 1 0.0 6 1 193 3 0.0 6 3 195 1 0.0 6 1 196 1 0.0 6 1 197 3 0.0 6 3 200 3 0.0 6 3 201 2 0.0 6 2 203 1 0.0 6 1 206 2 0.0 6 2 207 2 0.0 6 2 208 2 0.0 6 2 209 2 0.0 6 2 210 2 0.0 6 2 216 1 0.0 6 1 222 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_16_79_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_16_306_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_16_79_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_16_306_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.09 s (24 µs/read; 2.45 M reads/minute). === Summary === Total read pairs processed: 85,377 Read 1 with adapter: 85,369 (100.0%) Read 2 with adapter: 85,322 (99.9%) == Read fate breakdown == Pairs that were too short: 21 (0.0%) Pairs discarded as untrimmed: 62 (0.1%) Pairs written (passing filters): 85,294 (99.9%) Total basepairs processed: 36,544,069 bp Read 1: 20,111,872 bp Read 2: 16,432,197 bp Total written (filtered): 5,593,041 bp (15.3%) Read 1: 2,944,594 bp Read 2: 2,648,447 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 82959 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 4 333.5 1 1 3 6 2 20.8 1 2 7 1 5.2 2 1 9 3 0.3 2 3 10 1 0.1 3 1 14 2 0.0 4 2 15 1 0.0 4 1 16 3 0.0 4 2 1 17 3 0.0 5 3 19 2 0.0 5 1 0 1 20 94 0.0 6 90 4 21 57 0.0 6 55 2 22 38 0.0 6 36 2 23 30 0.0 6 27 2 1 24 23 0.0 6 20 2 1 25 20 0.0 6 20 26 21 0.0 6 19 2 27 14 0.0 6 13 1 28 9 0.0 6 8 0 0 1 29 8 0.0 6 8 30 10 0.0 6 9 1 31 11 0.0 6 11 32 7 0.0 6 7 33 7 0.0 6 6 0 0 1 34 7 0.0 6 7 35 5 0.0 6 5 36 12 0.0 6 11 1 37 5 0.0 6 4 1 38 9 0.0 6 8 1 39 11 0.0 6 11 40 8 0.0 6 5 3 41 8 0.0 6 8 42 5 0.0 6 5 43 8 0.0 6 8 44 2 0.0 6 2 45 7 0.0 6 7 46 6 0.0 6 6 47 3 0.0 6 3 48 6 0.0 6 6 49 6 0.0 6 6 50 5 0.0 6 5 51 5 0.0 6 4 1 52 12 0.0 6 12 53 4 0.0 6 4 54 4 0.0 6 3 1 55 5 0.0 6 4 1 56 8 0.0 6 8 57 4 0.0 6 4 58 12 0.0 6 12 59 4 0.0 6 4 60 13 0.0 6 13 61 4 0.0 6 4 62 1 0.0 6 1 63 6 0.0 6 6 64 7 0.0 6 7 65 7 0.0 6 6 1 66 4 0.0 6 4 67 8 0.0 6 8 68 8 0.0 6 8 69 10 0.0 6 10 70 10 0.0 6 10 71 12 0.0 6 12 72 10 0.0 6 9 1 73 11 0.0 6 10 0 1 74 6 0.0 6 6 75 5 0.0 6 5 76 4 0.0 6 3 1 77 18 0.0 6 18 78 4 0.0 6 4 79 9 0.0 6 9 80 7 0.0 6 7 81 8 0.0 6 8 82 11 0.0 6 11 83 13 0.0 6 13 84 14 0.0 6 13 1 85 11 0.0 6 11 86 11 0.0 6 11 87 11 0.0 6 11 88 11 0.0 6 11 89 11 0.0 6 11 90 2 0.0 6 1 0 1 91 8 0.0 6 8 92 10 0.0 6 10 93 5 0.0 6 5 94 5 0.0 6 5 95 9 0.0 6 9 96 12 0.0 6 12 97 14 0.0 6 12 2 98 15 0.0 6 15 99 20 0.0 6 20 100 14 0.0 6 14 101 22 0.0 6 21 1 102 16 0.0 6 16 103 28 0.0 6 28 104 17 0.0 6 17 105 19 0.0 6 18 1 106 20 0.0 6 19 0 0 1 107 15 0.0 6 15 108 20 0.0 6 20 109 33 0.0 6 33 110 27 0.0 6 27 111 38 0.0 6 37 1 112 34 0.0 6 34 113 31 0.0 6 30 1 114 37 0.0 6 37 115 30 0.0 6 27 3 116 25 0.0 6 25 117 30 0.0 6 30 118 22 0.0 6 22 119 26 0.0 6 26 120 29 0.0 6 28 1 121 30 0.0 6 29 0 0 0 0 0 1 122 35 0.0 6 32 2 0 1 123 41 0.0 6 40 1 124 32 0.0 6 31 1 125 39 0.0 6 37 2 126 27 0.0 6 26 1 127 39 0.0 6 39 128 46 0.0 6 45 1 129 40 0.0 6 39 1 130 41 0.0 6 40 0 0 0 1 131 55 0.0 6 55 132 43 0.0 6 42 1 133 55 0.0 6 54 1 134 49 0.0 6 49 135 54 0.0 6 52 2 136 53 0.0 6 53 137 74 0.0 6 74 138 76 0.0 6 75 1 139 84 0.0 6 81 2 0 1 140 131 0.0 6 130 1 141 357 0.0 6 353 4 142 643 0.0 6 643 143 756 0.0 6 752 4 144 1019 0.0 6 1018 1 145 1315 0.0 6 1313 2 146 1177 0.0 6 1175 1 0 0 0 0 1 147 856 0.0 6 853 3 148 1111 0.0 6 1109 1 0 0 0 1 149 895 0.0 6 892 3 150 946 0.0 6 942 4 151 1205 0.0 6 1203 1 0 0 0 0 1 152 749 0.0 6 749 153 707 0.0 6 704 3 154 354 0.0 6 353 1 155 428 0.0 6 425 2 0 0 1 156 584 0.0 6 584 157 428 0.0 6 427 1 158 430 0.0 6 424 6 159 864 0.0 6 862 2 160 644 0.0 6 639 5 161 894 0.0 6 893 1 162 371 0.0 6 368 3 163 323 0.0 6 321 1 1 164 752 0.0 6 749 3 165 384 0.0 6 381 3 166 432 0.0 6 429 3 167 365 0.0 6 360 4 0 0 0 0 1 168 447 0.0 6 446 1 169 266 0.0 6 266 170 472 0.0 6 466 5 0 1 171 591 0.0 6 589 2 172 347 0.0 6 345 1 0 0 0 0 1 173 727 0.0 6 724 2 0 0 0 1 174 608 0.0 6 605 2 0 1 175 683 0.0 6 682 1 176 509 0.0 6 507 2 177 613 0.0 6 612 1 178 397 0.0 6 395 2 179 663 0.0 6 658 4 0 0 1 180 964 0.0 6 963 1 181 607 0.0 6 604 3 182 505 0.0 6 500 4 0 0 0 0 1 183 600 0.0 6 598 1 0 0 0 1 184 342 0.0 6 340 2 185 431 0.0 6 428 2 0 0 1 186 395 0.0 6 391 4 187 313 0.0 6 313 188 274 0.0 6 273 1 189 340 0.0 6 336 4 190 326 0.0 6 323 2 0 0 0 1 191 487 0.0 6 484 2 0 1 192 458 0.0 6 454 4 193 609 0.0 6 602 7 194 620 0.0 6 614 5 1 195 736 0.0 6 727 8 1 196 555 0.0 6 549 6 197 721 0.0 6 716 5 198 575 0.0 6 571 3 0 0 1 199 427 0.0 6 422 4 1 200 633 0.0 6 627 5 1 201 618 0.0 6 614 4 202 700 0.0 6 692 8 203 662 0.0 6 658 3 0 0 0 1 204 595 0.0 6 591 3 0 0 0 1 205 1009 0.0 6 1001 8 206 706 0.0 6 699 6 0 0 0 1 207 876 0.0 6 871 5 208 649 0.0 6 645 4 209 640 0.0 6 634 6 210 749 0.0 6 742 7 211 796 0.0 6 793 3 212 688 0.0 6 681 4 1 0 1 0 1 213 821 0.0 6 813 6 0 0 1 0 1 214 986 0.0 6 982 4 215 810 0.0 6 808 2 216 641 0.0 6 633 8 217 739 0.0 6 730 8 0 0 1 218 789 0.0 6 780 8 0 0 0 0 1 219 620 0.0 6 613 6 1 220 673 0.0 6 666 7 221 551 0.0 6 550 1 222 658 0.0 6 647 10 0 0 0 1 223 620 0.0 6 615 5 224 717 0.0 6 707 10 225 646 0.0 6 636 8 2 226 665 0.0 6 661 3 1 227 599 0.0 6 595 3 0 0 1 228 533 0.0 6 525 7 0 1 229 460 0.0 6 450 10 230 505 0.0 6 496 8 0 0 1 231 469 0.0 6 465 3 1 232 537 0.0 6 531 6 233 524 0.0 6 516 8 234 429 0.0 6 424 5 235 452 0.0 6 447 5 236 453 0.0 6 452 1 237 472 0.0 6 465 7 238 448 0.0 6 442 5 1 239 483 0.0 6 480 2 1 240 545 0.0 6 542 3 241 509 0.0 6 505 3 0 0 0 1 242 451 0.0 6 441 8 1 0 1 243 415 0.0 6 412 3 244 365 0.0 6 362 3 245 437 0.0 6 429 8 246 297 0.0 6 294 3 247 374 0.0 6 368 6 248 349 0.0 6 341 8 249 334 0.0 6 332 2 250 268 0.0 6 264 3 1 251 340 0.0 6 332 7 1 252 239 0.0 6 234 4 0 1 253 244 0.0 6 241 3 254 358 0.0 6 348 10 255 280 0.0 6 275 5 256 259 0.0 6 256 3 257 306 0.0 6 296 10 258 341 0.0 6 332 9 259 399 0.0 6 391 8 260 314 0.0 6 306 7 0 0 1 261 237 0.0 6 231 6 262 328 0.0 6 321 6 0 0 1 263 436 0.0 6 429 7 264 274 0.0 6 267 7 265 332 0.0 6 327 3 2 266 239 0.0 6 233 6 267 325 0.0 6 313 12 268 409 0.0 6 403 5 1 269 228 0.0 6 223 5 270 181 0.0 6 175 6 271 248 0.0 6 238 10 272 614 0.0 6 604 10 273 419 0.0 6 407 11 1 274 496 0.0 6 487 9 275 465 0.0 6 450 14 1 276 670 0.0 6 654 15 1 277 2231 0.0 6 2183 45 2 1 278 3623 0.0 6 3543 79 1 279 67 0.0 6 62 5 280 10 0.0 6 10 281 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2410 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 1 0.0 6 1 63 3 0.0 6 1 2 64 199 0.0 6 192 7 65 2 0.0 6 1 1 70 1 0.0 6 0 0 0 0 0 0 1 72 2 0.0 6 2 73 13 0.0 6 13 74 20 0.0 6 20 75 27 0.0 6 27 76 39 0.0 6 39 77 60 0.0 6 60 78 39 0.0 6 39 79 35 0.0 6 35 80 58 0.0 6 58 81 37 0.0 6 37 82 33 0.0 6 33 83 53 0.0 6 53 84 38 0.0 6 37 1 85 24 0.0 6 24 86 14 0.0 6 14 87 16 0.0 6 15 1 88 26 0.0 6 26 89 12 0.0 6 12 90 25 0.0 6 25 91 35 0.0 6 34 1 92 23 0.0 6 23 93 23 0.0 6 23 94 22 0.0 6 22 95 8 0.0 6 7 1 96 18 0.0 6 18 97 17 0.0 6 17 98 14 0.0 6 14 99 17 0.0 6 17 100 7 0.0 6 7 101 11 0.0 6 11 102 10 0.0 6 10 103 20 0.0 6 20 104 19 0.0 6 19 105 25 0.0 6 25 106 16 0.0 6 16 107 23 0.0 6 23 108 17 0.0 6 17 109 23 0.0 6 23 110 11 0.0 6 11 111 24 0.0 6 24 112 34 0.0 6 34 113 18 0.0 6 18 114 14 0.0 6 14 115 13 0.0 6 13 116 15 0.0 6 15 117 8 0.0 6 8 118 7 0.0 6 7 119 8 0.0 6 8 120 7 0.0 6 7 121 4 0.0 6 4 122 5 0.0 6 5 123 8 0.0 6 8 124 3 0.0 6 3 125 16 0.0 6 16 126 12 0.0 6 12 127 10 0.0 6 10 128 7 0.0 6 7 129 9 0.0 6 9 130 10 0.0 6 10 131 2 0.0 6 2 132 13 0.0 6 12 1 133 13 0.0 6 13 134 16 0.0 6 16 135 10 0.0 6 9 1 136 10 0.0 6 10 137 13 0.0 6 13 138 12 0.0 6 11 1 139 17 0.0 6 17 140 13 0.0 6 13 141 15 0.0 6 15 142 16 0.0 6 16 143 16 0.0 6 16 144 3 0.0 6 3 145 19 0.0 6 19 146 24 0.0 6 24 147 21 0.0 6 21 148 11 0.0 6 10 0 0 0 0 0 1 149 5 0.0 6 5 150 14 0.0 6 14 151 14 0.0 6 14 152 10 0.0 6 9 1 153 9 0.0 6 9 154 7 0.0 6 7 155 10 0.0 6 10 156 27 0.0 6 27 157 10 0.0 6 10 158 14 0.0 6 14 159 6 0.0 6 6 160 5 0.0 6 5 161 2 0.0 6 2 162 8 0.0 6 8 163 4 0.0 6 4 164 4 0.0 6 4 165 7 0.0 6 7 166 12 0.0 6 12 167 9 0.0 6 8 1 168 12 0.0 6 12 169 11 0.0 6 11 170 13 0.0 6 13 171 15 0.0 6 15 172 15 0.0 6 15 173 22 0.0 6 22 174 21 0.0 6 21 175 4 0.0 6 4 176 5 0.0 6 5 177 8 0.0 6 8 178 8 0.0 6 8 179 12 0.0 6 12 180 15 0.0 6 15 181 3 0.0 6 3 182 12 0.0 6 12 183 11 0.0 6 11 184 4 0.0 6 4 185 10 0.0 6 10 186 7 0.0 6 7 187 6 0.0 6 6 188 5 0.0 6 5 189 10 0.0 6 10 190 5 0.0 6 5 191 12 0.0 6 12 192 11 0.0 6 11 193 8 0.0 6 8 194 3 0.0 6 3 195 1 0.0 6 1 196 8 0.0 6 8 197 9 0.0 6 9 198 8 0.0 6 8 200 2 0.0 6 2 201 5 0.0 6 5 202 5 0.0 6 5 203 7 0.0 6 7 204 8 0.0 6 8 205 8 0.0 6 8 206 9 0.0 6 9 207 7 0.0 6 7 208 7 0.0 6 7 209 3 0.0 6 3 210 6 0.0 6 6 211 13 0.0 6 13 212 8 0.0 6 8 213 13 0.0 6 13 214 8 0.0 6 8 215 1 0.0 6 1 216 6 0.0 6 6 218 3 0.0 6 3 219 12 0.0 6 12 220 5 0.0 6 5 221 4 0.0 6 4 222 2 0.0 6 2 223 2 0.0 6 2 224 5 0.0 6 5 225 5 0.0 6 5 226 1 0.0 6 1 227 3 0.0 6 3 228 5 0.0 6 5 229 11 0.0 6 11 230 11 0.0 6 11 231 3 0.0 6 3 232 4 0.0 6 4 233 7 0.0 6 7 234 3 0.0 6 3 235 5 0.0 6 5 236 12 0.0 6 12 237 3 0.0 6 3 238 8 0.0 6 8 239 6 0.0 6 6 240 5 0.0 6 5 241 2 0.0 6 2 242 1 0.0 6 1 244 2 0.0 6 2 245 1 0.0 6 1 246 3 0.0 6 3 247 2 0.0 6 2 248 2 0.0 6 2 250 1 0.0 6 1 251 4 0.0 6 4 252 1 0.0 6 1 253 3 0.0 6 3 254 3 0.0 6 3 255 5 0.0 6 5 256 5 0.0 6 5 257 1 0.0 6 1 258 6 0.0 6 6 259 4 0.0 6 4 261 1 0.0 6 1 263 1 0.0 6 1 266 1 0.0 6 1 268 1 0.0 6 1 269 2 0.0 6 2 270 2 0.0 6 2 271 1 0.0 6 1 272 1 0.0 6 1 273 3 0.0 6 3 274 7 0.0 6 7 278 2 0.0 6 2 279 2 0.0 6 2 280 1 0.0 6 1 281 1 0.0 6 1 282 1 0.0 6 1 284 2 0.0 6 2 285 4 0.0 6 4 286 1 0.0 6 1 289 1 0.0 6 1 291 1 0.0 6 1 297 1 0.0 6 1 298 2 0.0 6 2 300 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 83299 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 5.2 2 0 1 11 2 0.0 3 2 12 5 0.0 3 4 1 13 14 0.0 3 14 14 8 0.0 4 7 1 15 1 0.0 4 0 1 16 11 0.0 4 9 1 0 1 17 13 0.0 5 12 1 18 3 0.0 5 3 19 11 0.0 5 11 20 85 0.0 6 84 1 21 39 0.0 6 37 2 22 33 0.0 6 33 23 20 0.0 6 20 24 20 0.0 6 19 1 25 20 0.0 6 19 1 26 11 0.0 6 11 27 11 0.0 6 11 28 15 0.0 6 15 29 15 0.0 6 13 2 30 12 0.0 6 11 1 31 13 0.0 6 9 3 0 0 0 0 1 32 16 0.0 6 15 1 33 11 0.0 6 9 1 1 34 9 0.0 6 9 35 13 0.0 6 11 1 1 36 10 0.0 6 10 37 14 0.0 6 13 1 38 16 0.0 6 14 2 39 9 0.0 6 8 1 40 11 0.0 6 11 41 16 0.0 6 14 2 42 10 0.0 6 10 43 7 0.0 6 5 1 0 0 0 1 44 18 0.0 6 18 45 11 0.0 6 10 1 46 16 0.0 6 14 1 0 1 47 16 0.0 6 15 0 0 0 0 0 1 48 10 0.0 6 10 49 12 0.0 6 12 50 22 0.0 6 22 51 16 0.0 6 15 1 52 15 0.0 6 13 2 53 23 0.0 6 22 1 54 12 0.0 6 12 55 16 0.0 6 16 56 13 0.0 6 12 1 57 25 0.0 6 22 1 1 1 58 21 0.0 6 19 2 59 27 0.0 6 23 3 0 0 0 1 60 14 0.0 6 14 61 25 0.0 6 24 1 62 25 0.0 6 23 1 0 0 1 63 21 0.0 6 21 64 30 0.0 6 29 1 65 30 0.0 6 29 0 1 66 33 0.0 6 28 5 67 35 0.0 6 33 2 68 51 0.0 6 50 0 0 0 1 69 138 0.0 6 138 70 64 0.0 6 61 3 71 71 0.0 6 68 3 72 42 0.0 6 40 2 73 58 0.0 6 56 2 74 32 0.0 6 30 2 75 28 0.0 6 28 76 40 0.0 6 37 3 77 35 0.0 6 34 1 78 42 0.0 6 40 2 79 39 0.0 6 36 3 80 45 0.0 6 44 1 81 54 0.0 6 49 5 82 43 0.0 6 40 2 0 0 0 0 1 83 44 0.0 6 42 1 1 84 61 0.0 6 59 2 85 80 0.0 6 78 2 86 47 0.0 6 46 0 0 1 87 42 0.0 6 40 1 0 0 1 88 35 0.0 6 33 2 89 51 0.0 6 48 3 90 37 0.0 6 34 3 91 43 0.0 6 42 1 92 65 0.0 6 61 2 2 93 76 0.0 6 72 4 94 60 0.0 6 60 95 64 0.0 6 62 2 96 66 0.0 6 63 2 0 0 0 0 1 97 82 0.0 6 79 1 1 1 98 90 0.0 6 86 3 0 1 99 63 0.0 6 62 0 0 0 0 0 1 100 81 0.0 6 79 1 1 101 73 0.0 6 72 1 102 80 0.0 6 75 4 0 1 103 87 0.0 6 83 2 1 1 104 83 0.0 6 79 3 0 0 1 105 103 0.0 6 101 2 106 185 0.0 6 181 3 1 107 96 0.0 6 91 5 108 67 0.0 6 67 109 110 0.0 6 102 6 1 1 110 107 0.0 6 107 111 114 0.0 6 113 0 0 0 1 112 116 0.0 6 114 2 113 135 0.0 6 134 1 114 142 0.0 6 137 5 115 132 0.0 6 128 4 116 141 0.0 6 141 117 142 0.0 6 136 5 1 118 137 0.0 6 134 3 119 178 0.0 6 174 3 1 120 183 0.0 6 178 5 121 186 0.0 6 178 6 1 0 1 122 156 0.0 6 154 2 123 187 0.0 6 184 2 0 0 0 0 1 124 210 0.0 6 198 10 2 125 225 0.0 6 222 3 126 232 0.0 6 230 1 0 0 1 127 315 0.0 6 308 7 128 289 0.0 6 280 9 129 293 0.0 6 284 9 130 339 0.0 6 331 8 131 343 0.0 6 333 9 0 1 132 432 0.0 6 422 9 1 133 421 0.0 6 406 14 0 0 0 1 134 450 0.0 6 442 7 1 135 496 0.0 6 483 11 0 0 1 1 136 469 0.0 6 452 15 0 0 0 0 2 137 539 0.0 6 531 7 1 138 428 0.0 6 422 6 139 464 0.0 6 452 11 0 0 0 1 140 555 0.0 6 547 7 0 1 141 836 0.0 6 825 11 142 1089 0.0 6 1074 13 1 0 0 1 143 1331 0.0 6 1313 14 2 1 0 0 1 144 1629 0.0 6 1610 17 0 0 1 1 145 1590 0.0 6 1578 11 1 146 1518 0.0 6 1503 10 2 0 1 0 2 147 1282 0.0 6 1272 8 0 1 0 0 1 148 1508 0.0 6 1494 12 0 1 1 149 1453 0.0 6 1441 12 150 1667 0.0 6 1646 14 5 2 151 1712 0.0 6 1697 9 3 1 0 0 2 152 1839 0.0 6 1825 13 0 0 1 153 1428 0.0 6 1410 15 1 2 154 1426 0.0 6 1405 15 2 0 1 1 2 155 1487 0.0 6 1468 14 0 1 1 0 3 156 1290 0.0 6 1270 16 2 1 1 157 1351 0.0 6 1330 18 0 0 1 1 1 158 1176 0.0 6 1154 21 1 159 1470 0.0 6 1457 10 1 1 0 1 160 1375 0.0 6 1360 11 0 2 1 0 1 161 1301 0.0 6 1284 15 0 0 2 162 1030 0.0 6 1013 15 2 163 1034 0.0 6 1013 18 1 0 0 2 164 955 0.0 6 939 15 1 165 921 0.0 6 911 8 0 0 2 166 936 0.0 6 925 11 167 836 0.0 6 822 11 2 0 0 1 168 1151 0.0 6 1134 15 1 1 169 830 0.0 6 820 7 1 1 0 1 170 1120 0.0 6 1108 8 1 1 0 0 2 171 1129 0.0 6 1116 10 2 0 1 172 874 0.0 6 865 7 0 0 0 0 2 173 1167 0.0 6 1157 7 3 174 1106 0.0 6 1094 11 0 1 175 1277 0.0 6 1262 13 0 1 0 0 1 176 995 0.0 6 986 9 177 1003 0.0 6 988 14 0 0 1 178 1007 0.0 6 996 10 0 1 179 911 0.0 6 902 8 0 0 0 1 180 1030 0.0 6 1016 11 1 1 0 0 1 181 770 0.0 6 749 20 1 182 598 0.0 6 591 6 1 183 713 0.0 6 709 3 1 184 521 0.0 6 514 4 0 3 185 809 0.0 6 802 5 1 0 1 186 1242 0.0 6 1217 25 187 996 0.0 6 974 17 2 1 0 0 2 188 784 0.0 6 768 15 1 189 747 0.0 6 734 12 1 190 916 0.0 6 892 22 0 0 1 1 191 807 0.0 6 790 15 1 0 1 192 627 0.0 6 615 11 0 0 0 1 193 569 0.0 6 558 11 194 575 0.0 6 564 11 195 642 0.0 6 625 17 196 967 0.0 6 944 20 1 0 2 197 378 0.0 6 363 12 2 0 0 1 198 934 0.0 6 903 31 199 1193 0.0 6 1167 23 1 1 1 200 491 0.0 6 484 7 201 461 0.0 6 455 6 202 366 0.0 6 359 6 0 0 1 203 355 0.0 6 350 4 1 204 373 0.0 6 366 7 205 203 0.0 6 201 2 206 232 0.0 6 229 3 207 196 0.0 6 194 2 208 237 0.0 6 234 3 209 191 0.0 6 187 4 210 179 0.0 6 176 2 0 0 1 211 185 0.0 6 178 7 212 153 0.0 6 149 4 213 119 0.0 6 117 1 0 1 214 195 0.0 6 191 4 215 163 0.0 6 155 7 0 0 0 0 1 216 268 0.0 6 258 10 217 214 0.0 6 208 6 218 189 0.0 6 182 6 1 219 184 0.0 6 181 3 220 123 0.0 6 119 2 2 221 166 0.0 6 160 6 222 194 0.0 6 190 4 223 340 0.0 6 329 10 0 0 1 224 114 0.0 6 112 2 225 148 0.0 6 143 5 226 100 0.0 6 97 3 227 75 0.0 6 74 1 228 75 0.0 6 75 229 77 0.0 6 74 3 230 68 0.0 6 66 2 231 70 0.0 6 68 2 232 69 0.0 6 68 1 233 103 0.0 6 100 3 234 49 0.0 6 45 3 1 235 19 0.0 6 19 236 14 0.0 6 14 237 12 0.0 6 11 1 238 3 0.0 6 3 239 8 0.0 6 8 240 24 0.0 6 23 1 241 28 0.0 6 27 1 242 38 0.0 6 36 2 243 49 0.0 6 46 2 0 0 1 244 29 0.0 6 27 2 245 18 0.0 6 17 1 246 13 0.0 6 13 247 10 0.0 6 10 248 9 0.0 6 8 1 249 7 0.0 6 6 1 250 11 0.0 6 10 1 251 6 0.0 6 6 252 3 0.0 6 3 253 4 0.0 6 4 255 1 0.0 6 1 276 1 0.0 6 1 278 1 0.0 6 1 279 5 0.0 6 5 280 14 0.0 6 14 281 89 0.0 6 87 1 0 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2023 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 50 2 0.0 6 2 61 1 0.0 6 1 68 1 0.0 6 1 69 3 0.0 6 3 70 11 0.0 6 11 71 31 0.0 6 31 72 35 0.0 6 35 73 45 0.0 6 45 74 58 0.0 6 58 75 44 0.0 6 44 76 25 0.0 6 24 0 0 0 1 77 42 0.0 6 42 78 46 0.0 6 45 0 0 1 79 43 0.0 6 43 80 91 0.0 6 91 81 29 0.0 6 29 82 25 0.0 6 25 83 13 0.0 6 12 0 0 0 1 84 13 0.0 6 13 85 22 0.0 6 21 1 86 11 0.0 6 11 87 20 0.0 6 20 88 46 0.0 6 46 89 28 0.0 6 28 90 37 0.0 6 37 91 12 0.0 6 11 0 0 1 92 11 0.0 6 10 0 0 0 1 93 26 0.0 6 26 94 17 0.0 6 17 95 14 0.0 6 13 1 96 12 0.0 6 11 0 0 0 1 97 13 0.0 6 13 98 8 0.0 6 8 99 19 0.0 6 19 100 24 0.0 6 24 101 4 0.0 6 4 102 27 0.0 6 27 103 15 0.0 6 15 104 17 0.0 6 17 105 21 0.0 6 20 0 0 1 106 29 0.0 6 29 107 13 0.0 6 13 108 24 0.0 6 24 109 46 0.0 6 45 0 0 0 1 110 24 0.0 6 24 111 14 0.0 6 14 112 15 0.0 6 15 113 4 0.0 6 4 114 8 0.0 6 8 115 2 0.0 6 2 116 5 0.0 6 5 117 10 0.0 6 10 118 9 0.0 6 9 119 4 0.0 6 4 120 11 0.0 6 11 121 10 0.0 6 10 122 10 0.0 6 10 123 18 0.0 6 18 124 13 0.0 6 13 125 13 0.0 6 12 1 126 9 0.0 6 9 127 10 0.0 6 9 0 0 0 1 128 2 0.0 6 2 129 9 0.0 6 9 130 9 0.0 6 9 131 9 0.0 6 9 132 15 0.0 6 15 133 7 0.0 6 7 134 19 0.0 6 19 135 74 0.0 6 74 136 22 0.0 6 21 0 0 0 1 137 9 0.0 6 9 138 7 0.0 6 7 139 14 0.0 6 14 140 19 0.0 6 18 1 141 9 0.0 6 9 142 22 0.0 6 22 143 15 0.0 6 15 144 10 0.0 6 10 145 3 0.0 6 3 146 4 0.0 6 4 147 21 0.0 6 21 148 14 0.0 6 14 149 17 0.0 6 17 150 9 0.0 6 9 151 13 0.0 6 13 152 17 0.0 6 17 153 19 0.0 6 19 154 8 0.0 6 8 155 12 0.0 6 12 156 3 0.0 6 3 157 3 0.0 6 3 158 5 0.0 6 5 159 2 0.0 6 2 160 4 0.0 6 4 161 8 0.0 6 8 162 13 0.0 6 13 163 11 0.0 6 11 164 7 0.0 6 7 165 17 0.0 6 16 1 166 11 0.0 6 11 167 14 0.0 6 14 168 12 0.0 6 12 169 14 0.0 6 14 170 11 0.0 6 11 171 13 0.0 6 13 172 6 0.0 6 6 173 8 0.0 6 8 174 10 0.0 6 10 175 10 0.0 6 10 176 5 0.0 6 5 177 17 0.0 6 17 178 6 0.0 6 5 1 179 6 0.0 6 6 180 5 0.0 6 5 181 1 0.0 6 1 182 5 0.0 6 5 183 2 0.0 6 2 184 1 0.0 6 1 185 9 0.0 6 9 186 12 0.0 6 12 187 8 0.0 6 8 188 7 0.0 6 7 189 9 0.0 6 9 190 3 0.0 6 3 191 1 0.0 6 1 193 3 0.0 6 3 194 5 0.0 6 5 195 3 0.0 6 3 196 1 0.0 6 1 197 2 0.0 6 2 198 2 0.0 6 2 199 2 0.0 6 2 200 2 0.0 6 2 201 5 0.0 6 5 202 5 0.0 6 5 203 5 0.0 6 5 204 1 0.0 6 1 205 2 0.0 6 2 206 1 0.0 6 1 207 1 0.0 6 1 208 7 0.0 6 7 209 4 0.0 6 4 210 1 0.0 6 1 211 2 0.0 6 2 212 1 0.0 6 1 215 2 0.0 6 2 216 4 0.0 6 4 217 3 0.0 6 3 220 1 0.0 6 1 221 1 0.0 6 1 222 1 0.0 6 1 223 1 0.0 6 1 227 1 0.0 6 1 229 1 0.0 6 1 233 1 0.0 6 1 267 1 0.0 6 1 270 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_17_80_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_17_307_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_17_80_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_17_307_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.48 s (37 µs/read; 1.63 M reads/minute). === Summary === Total read pairs processed: 40,120 Read 1 with adapter: 40,118 (100.0%) Read 2 with adapter: 40,107 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 15 (0.0%) Pairs written (passing filters): 40,105 (100.0%) Total basepairs processed: 20,231,142 bp Read 1: 11,514,268 bp Read 2: 8,716,874 bp Total written (filtered): 2,489,695 bp (12.3%) Read 1: 1,324,566 bp Read 2: 1,165,129 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 40091 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 626.9 0 1 7 2 2.4 2 1 0 1 9 1 0.2 2 1 14 1 0.0 4 1 19 1 0.0 5 0 1 20 8 0.0 6 8 21 3 0.0 6 3 22 5 0.0 6 4 1 23 2 0.0 6 2 24 1 0.0 6 1 25 6 0.0 6 5 1 26 2 0.0 6 2 27 1 0.0 6 1 31 1 0.0 6 1 32 3 0.0 6 2 1 33 2 0.0 6 2 34 1 0.0 6 1 36 1 0.0 6 1 37 4 0.0 6 3 1 38 3 0.0 6 3 40 2 0.0 6 2 41 1 0.0 6 1 42 1 0.0 6 1 43 1 0.0 6 1 44 3 0.0 6 3 46 1 0.0 6 1 47 1 0.0 6 1 48 1 0.0 6 0 0 0 0 0 0 1 50 3 0.0 6 2 1 53 1 0.0 6 1 55 2 0.0 6 1 0 0 0 0 0 1 56 3 0.0 6 3 57 2 0.0 6 2 58 2 0.0 6 2 59 2 0.0 6 2 60 1 0.0 6 1 61 2 0.0 6 2 62 3 0.0 6 3 63 1 0.0 6 1 65 2 0.0 6 0 1 1 68 1 0.0 6 0 0 1 72 1 0.0 6 1 73 2 0.0 6 2 77 1 0.0 6 0 1 80 1 0.0 6 1 82 2 0.0 6 2 83 3 0.0 6 2 1 86 5 0.0 6 5 87 2 0.0 6 2 90 1 0.0 6 0 1 91 2 0.0 6 2 94 1 0.0 6 0 1 96 5 0.0 6 5 97 4 0.0 6 4 98 3 0.0 6 3 99 1 0.0 6 0 1 100 2 0.0 6 2 101 6 0.0 6 5 0 1 102 5 0.0 6 5 103 1 0.0 6 1 104 2 0.0 6 2 105 3 0.0 6 3 107 3 0.0 6 2 1 108 10 0.0 6 9 1 109 3 0.0 6 2 1 110 1 0.0 6 1 111 9 0.0 6 9 112 5 0.0 6 4 1 113 6 0.0 6 6 114 1 0.0 6 1 115 3 0.0 6 3 116 3 0.0 6 3 117 4 0.0 6 4 118 3 0.0 6 3 119 3 0.0 6 3 120 3 0.0 6 2 1 121 6 0.0 6 6 122 17 0.0 6 16 1 123 16 0.0 6 15 1 124 8 0.0 6 8 125 5 0.0 6 5 126 7 0.0 6 7 127 5 0.0 6 5 128 16 0.0 6 15 1 129 16 0.0 6 16 130 8 0.0 6 8 131 10 0.0 6 10 132 11 0.0 6 11 133 3 0.0 6 3 134 18 0.0 6 18 135 14 0.0 6 13 0 1 136 10 0.0 6 10 137 2 0.0 6 2 138 14 0.0 6 13 1 139 13 0.0 6 13 140 80 0.0 6 79 1 141 22 0.0 6 22 142 39 0.0 6 38 1 143 68 0.0 6 66 2 144 53 0.0 6 51 2 145 15 0.0 6 14 1 146 43 0.0 6 43 147 19 0.0 6 19 148 18 0.0 6 15 2 1 149 12 0.0 6 12 150 15 0.0 6 15 151 18 0.0 6 16 2 152 15 0.0 6 15 153 96 0.0 6 96 154 34 0.0 6 32 2 155 10 0.0 6 9 1 156 46 0.0 6 46 157 9 0.0 6 8 1 158 8 0.0 6 8 159 4 0.0 6 4 160 25 0.0 6 24 1 161 19 0.0 6 19 162 6 0.0 6 6 163 16 0.0 6 16 164 21 0.0 6 21 165 14 0.0 6 13 1 166 14 0.0 6 14 167 27 0.0 6 26 1 168 22 0.0 6 21 1 169 26 0.0 6 26 170 48 0.0 6 48 171 68 0.0 6 65 3 172 23 0.0 6 23 173 36 0.0 6 35 1 174 19 0.0 6 19 175 34 0.0 6 33 1 176 27 0.0 6 26 1 177 46 0.0 6 45 1 178 20 0.0 6 19 1 179 29 0.0 6 29 180 72 0.0 6 71 1 181 23 0.0 6 23 182 18 0.0 6 17 1 183 41 0.0 6 41 184 57 0.0 6 55 2 185 70 0.0 6 68 2 186 55 0.0 6 54 1 187 49 0.0 6 48 1 188 81 0.0 6 79 2 189 109 0.0 6 104 5 190 42 0.0 6 39 3 191 90 0.0 6 89 1 192 138 0.0 6 133 5 193 134 0.0 6 131 3 194 127 0.0 6 120 7 195 172 0.0 6 167 5 196 227 0.0 6 213 14 197 171 0.0 6 165 5 0 0 0 1 198 177 0.0 6 174 3 199 122 0.0 6 119 2 1 200 79 0.0 6 78 1 201 105 0.0 6 103 2 202 154 0.0 6 150 4 203 161 0.0 6 158 3 204 156 0.0 6 151 5 205 218 0.0 6 212 6 206 191 0.0 6 187 3 1 207 245 0.0 6 238 6 1 208 214 0.0 6 204 9 1 209 182 0.0 6 177 5 210 174 0.0 6 171 3 211 126 0.0 6 126 212 85 0.0 6 79 6 213 141 0.0 6 135 6 214 136 0.0 6 134 2 215 126 0.0 6 124 2 216 147 0.0 6 142 4 1 217 241 0.0 6 239 2 218 152 0.0 6 149 3 219 104 0.0 6 102 2 220 102 0.0 6 101 1 221 88 0.0 6 86 2 222 181 0.0 6 177 4 223 52 0.0 6 51 1 224 82 0.0 6 79 3 225 124 0.0 6 115 9 226 340 0.0 6 336 4 227 204 0.0 6 197 7 228 189 0.0 6 187 2 229 180 0.0 6 177 2 1 230 158 0.0 6 156 2 231 105 0.0 6 104 1 232 133 0.0 6 129 4 233 89 0.0 6 86 2 1 234 82 0.0 6 81 1 235 62 0.0 6 62 236 47 0.0 6 45 2 237 104 0.0 6 103 1 238 73 0.0 6 73 239 130 0.0 6 124 5 0 1 240 257 0.0 6 250 7 241 581 0.0 6 565 16 242 391 0.0 6 383 7 1 243 515 0.0 6 500 15 244 244 0.0 6 239 5 245 482 0.0 6 469 13 246 121 0.0 6 119 2 247 222 0.0 6 217 5 248 89 0.0 6 86 3 249 409 0.0 6 400 9 250 236 0.0 6 233 3 251 274 0.0 6 270 4 252 197 0.0 6 191 6 253 155 0.0 6 143 11 1 254 344 0.0 6 342 2 255 245 0.0 6 241 4 256 169 0.0 6 161 8 257 252 0.0 6 246 6 258 362 0.0 6 360 2 259 306 0.0 6 296 10 260 296 0.0 6 289 7 261 163 0.0 6 159 4 262 307 0.0 6 297 10 263 480 0.0 6 473 7 264 223 0.0 6 218 5 265 354 0.0 6 350 4 266 113 0.0 6 108 4 0 0 1 267 312 0.0 6 307 5 268 327 0.0 6 321 6 269 310 0.0 6 304 6 270 84 0.0 6 81 3 271 295 0.0 6 280 14 1 272 1463 0.0 6 1438 23 2 273 540 0.0 6 522 17 0 1 274 1268 0.0 6 1252 15 1 275 239 0.0 6 220 18 1 276 989 0.0 6 941 46 2 277 9699 0.0 6 9562 135 1 1 278 7306 0.0 6 7033 256 14 3 279 252 0.0 6 240 12 280 23 0.0 6 18 3 2 281 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 27 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 73 1 0.0 6 1 74 1 0.0 6 1 75 1 0.0 6 1 78 2 0.0 6 2 83 1 0.0 6 1 85 2 0.0 6 2 86 1 0.0 6 1 89 3 0.0 6 3 103 6 0.0 6 6 112 1 0.0 6 1 113 2 0.0 6 2 149 1 0.0 6 1 151 1 0.0 6 1 153 1 0.0 6 1 183 1 0.0 6 1 185 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 40068 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 9.8 1 1 7 1 2.4 2 1 12 4 0.0 3 4 13 2 0.0 3 2 14 4 0.0 4 4 15 1 0.0 4 1 16 1 0.0 4 1 17 5 0.0 5 4 0 1 18 1 0.0 5 0 1 19 12 0.0 5 9 2 1 20 38 0.0 6 36 2 21 27 0.0 6 26 1 22 24 0.0 6 23 1 23 18 0.0 6 16 1 1 24 11 0.0 6 10 1 25 8 0.0 6 7 1 26 4 0.0 6 4 27 3 0.0 6 3 28 8 0.0 6 7 1 29 4 0.0 6 4 30 6 0.0 6 6 31 3 0.0 6 3 32 6 0.0 6 3 3 33 7 0.0 6 5 2 34 3 0.0 6 3 35 5 0.0 6 5 36 3 0.0 6 3 37 6 0.0 6 5 1 38 7 0.0 6 6 1 39 5 0.0 6 4 1 40 4 0.0 6 4 41 7 0.0 6 6 1 42 4 0.0 6 4 43 3 0.0 6 2 1 44 3 0.0 6 2 0 0 1 45 5 0.0 6 4 1 46 4 0.0 6 4 47 7 0.0 6 7 48 11 0.0 6 10 1 49 18 0.0 6 18 50 12 0.0 6 11 1 51 8 0.0 6 6 2 52 12 0.0 6 10 1 1 53 6 0.0 6 4 2 54 5 0.0 6 5 55 17 0.0 6 15 2 56 20 0.0 6 18 2 57 18 0.0 6 17 1 58 12 0.0 6 10 2 59 5 0.0 6 5 60 8 0.0 6 8 61 9 0.0 6 9 62 9 0.0 6 5 3 1 63 11 0.0 6 11 64 7 0.0 6 7 65 2 0.0 6 1 1 66 8 0.0 6 6 2 67 10 0.0 6 9 1 68 8 0.0 6 8 69 14 0.0 6 12 1 1 70 74 0.0 6 72 2 71 11 0.0 6 10 1 72 42 0.0 6 41 1 73 6 0.0 6 5 1 74 23 0.0 6 23 75 14 0.0 6 14 76 8 0.0 6 6 2 77 13 0.0 6 12 0 1 78 10 0.0 6 9 1 79 8 0.0 6 6 2 80 23 0.0 6 23 81 13 0.0 6 12 1 82 14 0.0 6 13 1 83 12 0.0 6 10 2 84 19 0.0 6 17 1 1 85 22 0.0 6 21 1 86 23 0.0 6 22 1 87 29 0.0 6 29 88 12 0.0 6 10 1 0 0 1 89 11 0.0 6 9 1 0 0 1 90 21 0.0 6 19 2 91 17 0.0 6 17 92 14 0.0 6 14 93 25 0.0 6 19 6 94 47 0.0 6 46 1 95 23 0.0 6 21 1 1 96 42 0.0 6 37 3 1 0 1 97 21 0.0 6 21 98 13 0.0 6 12 0 1 99 36 0.0 6 31 5 100 22 0.0 6 19 3 101 29 0.0 6 29 102 31 0.0 6 28 2 0 0 1 103 34 0.0 6 31 3 104 34 0.0 6 31 3 105 44 0.0 6 41 3 106 21 0.0 6 20 0 0 1 107 25 0.0 6 24 1 108 27 0.0 6 23 4 109 19 0.0 6 17 2 110 23 0.0 6 21 2 111 32 0.0 6 32 112 31 0.0 6 26 2 2 0 0 1 113 19 0.0 6 18 1 114 29 0.0 6 27 0 1 0 0 1 115 36 0.0 6 33 2 1 116 21 0.0 6 19 2 117 20 0.0 6 19 1 118 31 0.0 6 28 3 119 49 0.0 6 45 3 0 0 1 120 31 0.0 6 28 2 1 121 24 0.0 6 21 1 1 0 1 122 33 0.0 6 33 123 28 0.0 6 27 0 0 0 1 124 38 0.0 6 37 1 125 31 0.0 6 30 1 126 22 0.0 6 22 127 31 0.0 6 31 128 27 0.0 6 26 1 129 33 0.0 6 33 130 21 0.0 6 21 131 38 0.0 6 35 3 132 44 0.0 6 41 1 2 133 24 0.0 6 23 1 134 60 0.0 6 54 6 135 125 0.0 6 117 8 136 61 0.0 6 59 2 137 104 0.0 6 101 2 1 138 41 0.0 6 40 1 139 79 0.0 6 79 140 115 0.0 6 109 5 0 0 0 1 141 162 0.0 6 157 5 142 151 0.0 6 147 4 143 154 0.0 6 147 6 0 0 1 144 236 0.0 6 230 5 0 0 0 1 145 116 0.0 6 112 4 146 93 0.0 6 90 1 1 0 1 147 70 0.0 6 70 148 89 0.0 6 87 2 149 152 0.0 6 148 2 2 150 197 0.0 6 190 6 1 151 112 0.0 6 107 5 152 265 0.0 6 254 10 0 0 1 153 274 0.0 6 266 7 1 154 257 0.0 6 246 11 155 226 0.0 6 219 7 156 173 0.0 6 163 8 1 1 157 262 0.0 6 244 18 158 249 0.0 6 244 5 159 104 0.0 6 95 9 160 246 0.0 6 238 8 161 155 0.0 6 151 3 0 0 1 162 136 0.0 6 134 1 0 0 1 163 240 0.0 6 232 8 164 231 0.0 6 222 9 165 266 0.0 6 253 12 0 0 1 166 316 0.0 6 304 12 167 165 0.0 6 154 11 168 355 0.0 6 343 11 1 169 127 0.0 6 123 4 170 110 0.0 6 104 6 171 270 0.0 6 262 7 0 0 1 172 132 0.0 6 126 5 1 173 228 0.0 6 220 6 2 174 306 0.0 6 289 17 175 634 0.0 6 618 16 176 427 0.0 6 416 9 1 1 177 361 0.0 6 347 10 3 0 0 0 1 178 339 0.0 6 327 11 0 0 1 179 334 0.0 6 317 15 0 0 1 1 180 317 0.0 6 304 13 181 466 0.0 6 451 13 0 0 2 182 215 0.0 6 206 9 183 563 0.0 6 555 8 184 85 0.0 6 81 3 1 185 98 0.0 6 91 7 186 489 0.0 6 479 10 187 646 0.0 6 616 29 1 188 888 0.0 6 862 23 1 1 1 189 674 0.0 6 657 14 3 190 1142 0.0 6 1122 20 191 902 0.0 6 867 34 0 1 192 625 0.0 6 609 14 1 0 1 193 458 0.0 6 437 19 2 194 715 0.0 6 691 19 4 0 1 195 770 0.0 6 742 27 0 0 1 196 2102 0.0 6 2042 58 0 1 1 197 429 0.0 6 410 18 1 198 967 0.0 6 935 29 1 1 1 199 2286 0.0 6 2233 52 1 200 606 0.0 6 593 12 0 0 1 201 1045 0.0 6 1021 23 0 0 1 202 538 0.0 6 521 16 0 1 203 361 0.0 6 346 13 2 204 563 0.0 6 548 15 205 194 0.0 6 189 5 206 235 0.0 6 229 6 207 145 0.0 6 141 4 208 235 0.0 6 234 1 209 334 0.0 6 327 7 210 265 0.0 6 258 6 1 211 449 0.0 6 432 17 212 489 0.0 6 474 15 213 72 0.0 6 66 6 214 207 0.0 6 202 5 215 200 0.0 6 195 5 216 356 0.0 6 344 12 217 901 0.0 6 887 14 218 368 0.0 6 359 8 0 0 0 0 1 219 428 0.0 6 413 12 0 0 3 220 271 0.0 6 265 5 1 221 501 0.0 6 490 11 222 211 0.0 6 208 3 223 342 0.0 6 333 7 2 224 288 0.0 6 275 13 225 462 0.0 6 451 11 226 413 0.0 6 403 9 0 0 1 227 324 0.0 6 318 6 228 175 0.0 6 169 6 229 397 0.0 6 389 8 230 172 0.0 6 166 5 1 231 272 0.0 6 266 6 232 244 0.0 6 235 9 233 310 0.0 6 304 5 0 0 1 234 136 0.0 6 134 2 235 144 0.0 6 140 4 236 39 0.0 6 37 2 237 63 0.0 6 60 3 238 19 0.0 6 18 1 239 24 0.0 6 24 240 84 0.0 6 81 2 1 241 139 0.0 6 136 3 242 167 0.0 6 165 2 243 151 0.0 6 149 2 244 207 0.0 6 199 6 1 0 0 1 245 48 0.0 6 47 1 246 27 0.0 6 26 1 247 14 0.0 6 13 1 248 31 0.0 6 31 249 18 0.0 6 18 250 10 0.0 6 10 251 24 0.0 6 23 1 252 4 0.0 6 4 253 8 0.0 6 7 1 254 18 0.0 6 18 255 1 0.0 6 1 256 6 0.0 6 6 257 3 0.0 6 3 258 6 0.0 6 5 1 259 1 0.0 6 1 260 1 0.0 6 1 263 1 0.0 6 1 265 1 0.0 6 1 278 1 0.0 6 1 281 5 0.0 6 5 282 9 0.0 6 9 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 39 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 68 3 0.0 6 3 69 1 0.0 6 1 70 1 0.0 6 1 71 2 0.0 6 2 72 4 0.0 6 4 73 1 0.0 6 1 75 5 0.0 6 5 80 1 0.0 6 1 81 1 0.0 6 1 82 8 0.0 6 8 84 1 0.0 6 1 85 3 0.0 6 3 86 1 0.0 6 1 92 1 0.0 6 1 99 2 0.0 6 2 136 1 0.0 6 1 145 2 0.0 6 2 146 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_18_81_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_18_308_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_18_81_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_18_308_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.31 s (28 µs/read; 2.13 M reads/minute). === Summary === Total read pairs processed: 46,572 Read 1 with adapter: 46,568 (100.0%) Read 2 with adapter: 46,548 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 28 (0.1%) Pairs written (passing filters): 46,544 (99.9%) Total basepairs processed: 23,511,568 bp Read 1: 13,407,356 bp Read 2: 10,104,212 bp Total written (filtered): 3,041,864 bp (12.9%) Read 1: 1,544,524 bp Read 2: 1,497,340 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 46427 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 2 11.4 1 0 0 2 7 1 2.8 2 0 0 1 15 1 0.0 4 1 16 2 0.0 4 2 20 22 0.0 6 21 1 21 7 0.0 6 7 22 7 0.0 6 4 3 23 4 0.0 6 4 24 6 0.0 6 6 25 6 0.0 6 6 26 4 0.0 6 4 27 2 0.0 6 2 30 1 0.0 6 1 31 2 0.0 6 2 32 1 0.0 6 1 33 3 0.0 6 3 36 1 0.0 6 1 38 2 0.0 6 2 39 5 0.0 6 5 40 4 0.0 6 4 44 4 0.0 6 4 45 3 0.0 6 3 47 2 0.0 6 2 49 3 0.0 6 2 0 0 0 0 0 1 50 2 0.0 6 2 55 2 0.0 6 2 56 1 0.0 6 1 57 7 0.0 6 6 1 58 3 0.0 6 3 59 1 0.0 6 1 60 2 0.0 6 2 63 3 0.0 6 3 64 7 0.0 6 7 65 2 0.0 6 2 66 1 0.0 6 1 67 1 0.0 6 1 68 2 0.0 6 1 0 1 69 1 0.0 6 1 70 2 0.0 6 2 73 2 0.0 6 2 75 1 0.0 6 1 77 5 0.0 6 4 1 78 4 0.0 6 4 82 1 0.0 6 1 83 2 0.0 6 2 84 3 0.0 6 3 86 3 0.0 6 3 87 3 0.0 6 3 88 2 0.0 6 2 89 2 0.0 6 2 90 2 0.0 6 2 91 6 0.0 6 5 0 0 0 0 1 92 3 0.0 6 2 1 93 2 0.0 6 2 94 1 0.0 6 1 95 3 0.0 6 3 96 2 0.0 6 1 1 97 2 0.0 6 2 98 3 0.0 6 3 99 1 0.0 6 0 1 100 6 0.0 6 5 0 0 0 1 101 3 0.0 6 2 1 102 11 0.0 6 9 2 104 7 0.0 6 7 105 5 0.0 6 5 106 1 0.0 6 1 107 2 0.0 6 2 108 9 0.0 6 9 109 1 0.0 6 1 110 1 0.0 6 1 111 3 0.0 6 3 112 5 0.0 6 4 1 113 6 0.0 6 6 114 3 0.0 6 2 1 115 6 0.0 6 6 116 5 0.0 6 4 1 117 7 0.0 6 5 2 118 4 0.0 6 4 119 3 0.0 6 3 120 2 0.0 6 2 121 6 0.0 6 5 1 122 14 0.0 6 14 123 14 0.0 6 13 1 124 7 0.0 6 7 125 7 0.0 6 7 126 11 0.0 6 11 127 8 0.0 6 7 0 0 1 128 18 0.0 6 15 3 129 16 0.0 6 15 1 130 8 0.0 6 8 131 7 0.0 6 7 132 5 0.0 6 5 133 12 0.0 6 11 0 1 134 14 0.0 6 13 1 135 33 0.0 6 32 1 136 25 0.0 6 25 137 26 0.0 6 25 1 138 18 0.0 6 18 139 20 0.0 6 20 140 30 0.0 6 28 2 141 36 0.0 6 32 4 142 18 0.0 6 18 143 130 0.0 6 129 1 144 137 0.0 6 136 1 145 238 0.0 6 237 1 146 259 0.0 6 259 147 57 0.0 6 57 148 65 0.0 6 64 1 149 358 0.0 6 358 150 64 0.0 6 63 1 151 85 0.0 6 85 152 37 0.0 6 36 1 153 51 0.0 6 50 1 154 25 0.0 6 25 155 27 0.0 6 27 156 42 0.0 6 41 1 157 59 0.0 6 58 1 158 107 0.0 6 107 159 76 0.0 6 76 160 58 0.0 6 58 161 433 0.0 6 433 162 87 0.0 6 87 163 202 0.0 6 201 1 164 116 0.0 6 115 1 165 100 0.0 6 100 166 34 0.0 6 34 167 42 0.0 6 42 168 243 0.0 6 242 1 169 77 0.0 6 74 3 170 215 0.0 6 213 2 171 89 0.0 6 86 3 172 63 0.0 6 62 1 173 36 0.0 6 35 1 174 40 0.0 6 39 1 175 47 0.0 6 46 1 176 26 0.0 6 25 1 177 19 0.0 6 19 178 26 0.0 6 24 2 179 26 0.0 6 26 180 40 0.0 6 38 2 181 153 0.0 6 150 3 182 119 0.0 6 115 4 183 45 0.0 6 41 3 0 1 184 66 0.0 6 63 3 185 109 0.0 6 104 5 186 102 0.0 6 96 6 187 92 0.0 6 91 1 188 72 0.0 6 71 1 189 61 0.0 6 58 3 190 64 0.0 6 59 5 191 48 0.0 6 46 2 192 64 0.0 6 60 4 193 104 0.0 6 101 3 194 71 0.0 6 68 3 195 144 0.0 6 139 5 196 139 0.0 6 136 3 197 148 0.0 6 141 6 1 198 111 0.0 6 106 4 0 0 1 199 115 0.0 6 106 8 1 200 124 0.0 6 120 4 201 86 0.0 6 82 4 202 129 0.0 6 125 4 203 108 0.0 6 96 11 1 204 145 0.0 6 139 6 205 123 0.0 6 118 5 206 128 0.0 6 124 4 207 136 0.0 6 130 6 208 89 0.0 6 88 1 209 70 0.0 6 68 1 1 210 92 0.0 6 89 3 211 91 0.0 6 90 1 212 104 0.0 6 103 1 213 103 0.0 6 97 6 214 162 0.0 6 159 3 215 84 0.0 6 80 4 216 82 0.0 6 78 4 217 100 0.0 6 97 3 218 99 0.0 6 93 6 219 110 0.0 6 108 2 220 99 0.0 6 97 1 0 1 221 58 0.0 6 58 222 74 0.0 6 72 2 223 142 0.0 6 137 5 224 141 0.0 6 129 12 225 137 0.0 6 134 3 226 120 0.0 6 112 8 227 99 0.0 6 97 2 228 73 0.0 6 70 3 229 80 0.0 6 77 3 230 77 0.0 6 73 4 231 79 0.0 6 75 4 232 67 0.0 6 66 1 233 57 0.0 6 56 1 234 59 0.0 6 57 1 0 1 235 34 0.0 6 33 1 236 75 0.0 6 72 3 237 99 0.0 6 93 5 1 238 187 0.0 6 173 13 1 239 131 0.0 6 120 11 240 176 0.0 6 165 10 1 241 144 0.0 6 139 5 242 197 0.0 6 190 7 243 73 0.0 6 66 6 1 244 94 0.0 6 86 5 2 1 245 82 0.0 6 76 4 2 246 182 0.0 6 177 5 247 99 0.0 6 95 4 248 132 0.0 6 125 7 249 109 0.0 6 104 4 0 1 250 77 0.0 6 71 5 1 251 147 0.0 6 142 4 1 252 85 0.0 6 80 5 253 66 0.0 6 64 2 254 72 0.0 6 67 5 255 88 0.0 6 81 7 256 134 0.0 6 127 7 257 165 0.0 6 159 6 258 64 0.0 6 55 8 1 259 122 0.0 6 114 8 260 199 0.0 6 192 7 261 87 0.0 6 82 5 262 99 0.0 6 97 2 263 50 0.0 6 48 2 264 69 0.0 6 65 4 265 81 0.0 6 72 9 266 107 0.0 6 102 5 267 104 0.0 6 101 2 1 268 94 0.0 6 74 20 269 218 0.0 6 211 7 270 122 0.0 6 113 9 271 142 0.0 6 136 6 272 125 0.0 6 113 11 1 273 257 0.0 6 222 34 1 274 521 0.0 6 501 20 275 238 0.0 6 223 9 5 1 276 490 0.0 6 468 21 0 1 277 876 0.0 6 747 119 9 1 278 29856 0.0 6 29210 637 3 3 0 0 3 279 33 0.0 6 32 0 0 1 280 3 0.0 6 2 0 0 1 281 4 0.0 6 1 2 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 141 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 2 0.0 6 2 72 1 0.0 6 1 73 6 0.0 6 6 74 4 0.0 6 4 75 4 0.0 6 4 76 10 0.0 6 10 78 3 0.0 6 3 79 14 0.0 6 14 80 3 0.0 6 3 81 1 0.0 6 1 82 6 0.0 6 6 83 2 0.0 6 2 85 1 0.0 6 1 86 1 0.0 6 1 87 1 0.0 6 1 88 2 0.0 6 2 89 1 0.0 6 1 90 5 0.0 6 5 91 12 0.0 6 12 92 2 0.0 6 2 93 4 0.0 6 4 94 3 0.0 6 3 95 2 0.0 6 2 97 3 0.0 6 3 98 8 0.0 6 8 100 7 0.0 6 7 101 4 0.0 6 4 102 2 0.0 6 2 105 2 0.0 6 2 110 1 0.0 6 1 111 5 0.0 6 5 114 1 0.0 6 1 120 1 0.0 6 1 132 1 0.0 6 1 134 1 0.0 6 1 151 2 0.0 6 2 158 1 0.0 6 1 159 2 0.0 6 2 165 1 0.0 6 1 167 1 0.0 6 1 173 1 0.0 6 1 175 1 0.0 6 1 181 1 0.0 6 1 183 3 0.0 6 3 185 1 0.0 6 1 189 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 46393 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 727.7 0 1 6 2 11.4 1 1 1 10 2 0.0 3 2 13 4 0.0 3 3 1 14 7 0.0 4 5 2 16 7 0.0 4 6 0 0 0 0 1 17 8 0.0 5 6 2 18 6 0.0 5 5 0 1 19 6 0.0 5 5 1 20 40 0.0 6 40 21 37 0.0 6 36 1 22 27 0.0 6 24 2 0 1 23 15 0.0 6 14 1 24 14 0.0 6 14 25 13 0.0 6 12 1 26 8 0.0 6 8 27 12 0.0 6 12 28 4 0.0 6 3 1 29 12 0.0 6 11 1 30 19 0.0 6 18 0 1 31 8 0.0 6 6 2 32 6 0.0 6 6 33 6 0.0 6 6 34 12 0.0 6 12 35 7 0.0 6 5 2 36 6 0.0 6 6 37 10 0.0 6 10 38 2 0.0 6 1 0 0 1 39 5 0.0 6 5 40 7 0.0 6 7 41 8 0.0 6 6 2 42 2 0.0 6 2 43 10 0.0 6 7 3 44 12 0.0 6 11 1 45 3 0.0 6 3 46 9 0.0 6 6 3 47 10 0.0 6 9 1 48 7 0.0 6 7 49 9 0.0 6 7 2 50 9 0.0 6 7 2 51 5 0.0 6 3 2 52 12 0.0 6 10 1 1 53 9 0.0 6 8 0 1 54 18 0.0 6 15 3 55 10 0.0 6 8 2 56 12 0.0 6 10 2 57 14 0.0 6 11 3 58 13 0.0 6 11 2 59 1 0.0 6 0 1 60 15 0.0 6 9 5 1 61 7 0.0 6 7 62 6 0.0 6 5 1 63 11 0.0 6 10 1 64 8 0.0 6 8 65 7 0.0 6 6 1 66 12 0.0 6 11 0 0 0 1 67 88 0.0 6 82 5 1 68 29 0.0 6 24 4 0 0 1 69 31 0.0 6 28 3 70 7 0.0 6 7 71 20 0.0 6 19 1 72 12 0.0 6 12 73 8 0.0 6 6 1 1 74 10 0.0 6 9 1 75 16 0.0 6 14 0 2 76 5 0.0 6 5 77 8 0.0 6 8 78 14 0.0 6 12 1 1 79 9 0.0 6 8 1 80 9 0.0 6 8 1 81 5 0.0 6 5 82 22 0.0 6 21 0 1 83 34 0.0 6 32 1 1 84 23 0.0 6 22 1 85 19 0.0 6 16 3 86 12 0.0 6 12 87 34 0.0 6 28 3 1 1 1 88 15 0.0 6 13 2 89 29 0.0 6 28 1 90 26 0.0 6 25 1 91 24 0.0 6 23 1 92 24 0.0 6 22 2 93 36 0.0 6 33 3 94 33 0.0 6 27 5 1 95 29 0.0 6 25 4 96 28 0.0 6 25 3 97 21 0.0 6 21 98 18 0.0 6 15 3 99 46 0.0 6 44 2 100 24 0.0 6 20 4 101 30 0.0 6 28 1 0 0 1 102 42 0.0 6 36 6 103 68 0.0 6 65 3 104 46 0.0 6 43 3 105 46 0.0 6 43 3 106 31 0.0 6 29 1 1 107 21 0.0 6 21 108 23 0.0 6 23 109 45 0.0 6 44 1 110 40 0.0 6 40 111 62 0.0 6 57 4 0 0 1 112 37 0.0 6 35 2 113 49 0.0 6 47 1 0 0 1 114 58 0.0 6 54 3 1 115 49 0.0 6 44 5 116 49 0.0 6 47 2 117 38 0.0 6 36 2 118 51 0.0 6 48 2 1 119 52 0.0 6 47 5 120 50 0.0 6 47 3 121 28 0.0 6 25 3 122 47 0.0 6 45 2 123 35 0.0 6 33 2 124 57 0.0 6 52 5 125 51 0.0 6 48 3 126 47 0.0 6 46 1 127 62 0.0 6 61 1 128 45 0.0 6 43 2 129 49 0.0 6 47 1 1 130 53 0.0 6 51 2 131 74 0.0 6 73 1 132 77 0.0 6 75 2 133 87 0.0 6 82 5 134 145 0.0 6 139 6 135 147 0.0 6 143 4 136 105 0.0 6 103 2 137 126 0.0 6 123 3 138 82 0.0 6 81 1 139 137 0.0 6 134 3 140 186 0.0 6 181 5 141 236 0.0 6 230 6 142 322 0.0 6 314 8 143 345 0.0 6 335 9 1 144 407 0.0 6 397 10 145 199 0.0 6 194 5 146 169 0.0 6 164 4 1 147 350 0.0 6 344 6 148 170 0.0 6 162 7 1 149 279 0.0 6 273 5 1 150 420 0.0 6 404 14 1 0 1 151 174 0.0 6 163 10 1 152 357 0.0 6 343 10 4 153 219 0.0 6 214 5 154 301 0.0 6 290 10 1 155 270 0.0 6 263 7 156 251 0.0 6 241 9 1 157 337 0.0 6 324 12 0 1 158 375 0.0 6 362 13 159 350 0.0 6 341 9 160 310 0.0 6 307 2 1 161 264 0.0 6 254 9 1 162 178 0.0 6 172 6 163 297 0.0 6 285 10 1 0 1 164 230 0.0 6 225 4 0 0 1 165 303 0.0 6 293 9 0 1 166 404 0.0 6 395 7 0 0 2 167 211 0.0 6 199 12 168 429 0.0 6 415 14 169 179 0.0 6 173 6 170 137 0.0 6 132 5 171 374 0.0 6 365 8 1 172 191 0.0 6 185 6 173 359 0.0 6 334 24 1 174 469 0.0 6 449 19 1 175 777 0.0 6 751 24 0 1 0 1 176 445 0.0 6 430 15 177 401 0.0 6 373 25 3 178 333 0.0 6 319 11 1 0 2 179 377 0.0 6 357 19 0 1 180 329 0.0 6 312 15 1 0 1 181 413 0.0 6 398 14 1 182 124 0.0 6 111 13 183 474 0.0 6 457 17 184 327 0.0 6 315 11 0 0 1 185 467 0.0 6 442 25 186 837 0.0 6 812 24 0 0 0 1 187 698 0.0 6 669 28 1 188 756 0.0 6 723 30 0 1 2 189 640 0.0 6 617 22 1 190 1018 0.0 6 982 36 191 951 0.0 6 912 37 0 1 1 192 469 0.0 6 457 11 0 0 1 193 337 0.0 6 323 14 194 670 0.0 6 650 19 1 195 1091 0.0 6 1043 48 196 2028 0.0 6 1948 76 3 0 1 197 538 0.0 6 500 34 2 1 0 0 1 198 1188 0.0 6 1134 49 3 0 2 199 1837 0.0 6 1787 47 1 1 1 200 727 0.0 6 698 27 1 0 0 1 201 1072 0.0 6 1020 50 0 0 2 202 520 0.0 6 499 19 1 0 1 203 510 0.0 6 481 26 3 204 679 0.0 6 659 20 205 312 0.0 6 303 9 206 253 0.0 6 245 6 0 0 2 207 110 0.0 6 105 5 208 265 0.0 6 255 10 209 241 0.0 6 231 10 210 210 0.0 6 200 10 211 148 0.0 6 142 6 212 319 0.0 6 307 11 1 213 152 0.0 6 144 7 1 214 550 0.0 6 525 21 3 0 1 215 497 0.0 6 480 15 2 216 431 0.0 6 414 17 217 475 0.0 6 458 16 1 218 358 0.0 6 346 11 1 219 542 0.0 6 532 9 0 0 0 1 220 119 0.0 6 115 4 221 553 0.0 6 527 26 222 404 0.0 6 386 18 223 876 0.0 6 839 36 1 224 378 0.0 6 368 9 1 225 497 0.0 6 486 11 226 324 0.0 6 313 11 227 303 0.0 6 295 8 228 156 0.0 6 148 8 229 419 0.0 6 408 11 230 230 0.0 6 222 8 231 333 0.0 6 324 9 232 426 0.0 6 409 17 233 243 0.0 6 237 6 234 127 0.0 6 120 7 235 88 0.0 6 81 6 1 236 23 0.0 6 22 0 0 0 1 237 30 0.0 6 29 1 238 27 0.0 6 27 239 42 0.0 6 39 3 240 99 0.0 6 98 1 241 163 0.0 6 155 7 1 242 201 0.0 6 195 6 243 119 0.0 6 116 3 244 149 0.0 6 146 3 245 74 0.0 6 68 6 246 121 0.0 6 113 8 247 43 0.0 6 43 248 119 0.0 6 118 1 249 30 0.0 6 29 1 250 17 0.0 6 17 251 16 0.0 6 16 252 2 0.0 6 2 253 3 0.0 6 3 254 3 0.0 6 2 1 276 1 0.0 6 1 278 4 0.0 6 4 279 22 0.0 6 21 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 155 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 73 6 0.0 6 6 74 8 0.0 6 8 75 7 0.0 6 7 76 1 0.0 6 0 0 0 0 1 77 2 0.0 6 2 78 14 0.0 6 14 79 1 0.0 6 1 80 2 0.0 6 2 81 1 0.0 6 1 84 3 0.0 6 3 86 2 0.0 6 2 87 6 0.0 6 6 88 2 0.0 6 2 89 5 0.0 6 5 90 15 0.0 6 15 91 2 0.0 6 2 92 10 0.0 6 10 93 4 0.0 6 4 94 2 0.0 6 2 96 3 0.0 6 3 97 4 0.0 6 4 98 1 0.0 6 1 99 11 0.0 6 11 104 2 0.0 6 2 109 2 0.0 6 2 110 5 0.0 6 5 111 2 0.0 6 2 114 3 0.0 6 3 115 2 0.0 6 2 126 1 0.0 6 1 127 1 0.0 6 1 128 1 0.0 6 1 130 1 0.0 6 1 140 2 0.0 6 2 141 1 0.0 6 1 149 8 0.0 6 8 150 1 0.0 6 1 171 1 0.0 6 1 172 1 0.0 6 1 174 3 0.0 6 3 180 2 0.0 6 2 182 2 0.0 6 2 188 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_19_82_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_19_309_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_19_82_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_19_309_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.02 s (29 µs/read; 2.05 M reads/minute). === Summary === Total read pairs processed: 34,734 Read 1 with adapter: 34,733 (100.0%) Read 2 with adapter: 34,723 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 12 (0.0%) Pairs written (passing filters): 34,722 (100.0%) Total basepairs processed: 17,918,199 bp Read 1: 10,177,847 bp Read 2: 7,740,352 bp Total written (filtered): 2,186,990 bp (12.2%) Read 1: 1,145,419 bp Read 2: 1,041,571 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 34733 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 542.7 0 2 5 1 33.9 1 1 6 1 8.5 1 0 0 1 14 2 0.0 4 0 1 0 0 1 20 5 0.0 6 4 1 21 2 0.0 6 2 22 1 0.0 6 1 25 2 0.0 6 2 26 2 0.0 6 2 27 1 0.0 6 1 29 1 0.0 6 1 31 1 0.0 6 1 32 1 0.0 6 1 33 2 0.0 6 2 37 2 0.0 6 2 40 2 0.0 6 2 41 2 0.0 6 1 1 44 4 0.0 6 4 45 1 0.0 6 1 46 1 0.0 6 1 47 1 0.0 6 1 49 1 0.0 6 1 50 2 0.0 6 1 1 53 1 0.0 6 1 54 1 0.0 6 1 55 1 0.0 6 1 57 1 0.0 6 1 58 1 0.0 6 1 59 3 0.0 6 2 1 64 2 0.0 6 2 67 1 0.0 6 0 1 68 1 0.0 6 1 69 1 0.0 6 1 71 1 0.0 6 1 74 1 0.0 6 1 78 2 0.0 6 2 79 1 0.0 6 1 80 1 0.0 6 1 81 1 0.0 6 1 86 5 0.0 6 5 88 1 0.0 6 1 90 4 0.0 6 4 91 1 0.0 6 1 93 2 0.0 6 2 94 2 0.0 6 2 97 4 0.0 6 4 100 3 0.0 6 3 101 2 0.0 6 1 1 102 1 0.0 6 1 104 2 0.0 6 1 0 1 105 3 0.0 6 3 107 3 0.0 6 3 108 2 0.0 6 2 111 3 0.0 6 3 112 6 0.0 6 4 2 113 5 0.0 6 5 114 1 0.0 6 1 115 2 0.0 6 2 116 2 0.0 6 1 1 117 2 0.0 6 2 118 4 0.0 6 4 119 2 0.0 6 2 120 3 0.0 6 2 0 1 121 1 0.0 6 1 122 8 0.0 6 8 123 6 0.0 6 6 124 6 0.0 6 6 125 6 0.0 6 6 126 7 0.0 6 7 127 7 0.0 6 7 128 10 0.0 6 10 129 4 0.0 6 4 130 4 0.0 6 3 1 131 5 0.0 6 5 132 6 0.0 6 6 133 3 0.0 6 3 134 14 0.0 6 14 135 8 0.0 6 8 136 8 0.0 6 8 137 9 0.0 6 8 0 1 138 8 0.0 6 7 1 139 8 0.0 6 7 0 0 1 140 3 0.0 6 3 141 7 0.0 6 7 142 3 0.0 6 3 143 8 0.0 6 8 144 7 0.0 6 7 145 12 0.0 6 11 1 146 2 0.0 6 2 147 6 0.0 6 6 148 12 0.0 6 12 149 7 0.0 6 7 150 12 0.0 6 12 151 9 0.0 6 8 0 0 1 152 9 0.0 6 9 153 6 0.0 6 6 154 6 0.0 6 6 155 4 0.0 6 4 156 10 0.0 6 10 157 3 0.0 6 3 158 6 0.0 6 6 159 6 0.0 6 6 160 9 0.0 6 8 1 161 8 0.0 6 8 162 9 0.0 6 9 163 5 0.0 6 5 164 13 0.0 6 13 165 9 0.0 6 9 166 10 0.0 6 9 1 167 14 0.0 6 12 2 168 10 0.0 6 10 169 9 0.0 6 7 2 170 22 0.0 6 21 1 171 21 0.0 6 21 172 8 0.0 6 8 173 13 0.0 6 11 2 174 19 0.0 6 17 2 175 12 0.0 6 12 176 11 0.0 6 11 177 12 0.0 6 11 1 178 16 0.0 6 15 1 179 17 0.0 6 17 180 19 0.0 6 18 1 181 17 0.0 6 16 1 182 29 0.0 6 28 1 183 21 0.0 6 21 184 24 0.0 6 22 2 185 35 0.0 6 35 186 40 0.0 6 40 187 26 0.0 6 25 1 188 40 0.0 6 40 189 32 0.0 6 32 190 38 0.0 6 38 191 51 0.0 6 50 1 192 50 0.0 6 50 193 67 0.0 6 64 3 194 61 0.0 6 60 1 195 68 0.0 6 65 3 196 71 0.0 6 67 4 197 45 0.0 6 43 2 198 40 0.0 6 39 1 199 45 0.0 6 44 1 200 61 0.0 6 60 1 201 50 0.0 6 48 2 202 54 0.0 6 50 4 203 63 0.0 6 62 1 204 67 0.0 6 65 2 205 81 0.0 6 80 1 206 85 0.0 6 83 2 207 79 0.0 6 74 5 208 100 0.0 6 98 2 209 109 0.0 6 104 4 1 210 128 0.0 6 123 4 1 211 238 0.0 6 233 5 212 89 0.0 6 84 4 1 213 150 0.0 6 147 2 0 1 214 209 0.0 6 203 5 1 215 85 0.0 6 82 2 1 216 173 0.0 6 169 4 217 125 0.0 6 124 1 218 81 0.0 6 77 3 1 219 85 0.0 6 81 4 220 105 0.0 6 104 1 221 92 0.0 6 88 4 222 150 0.0 6 147 3 223 176 0.0 6 172 4 224 206 0.0 6 198 8 225 122 0.0 6 121 1 226 322 0.0 6 315 7 227 148 0.0 6 143 5 228 123 0.0 6 119 4 229 201 0.0 6 200 1 230 111 0.0 6 111 231 81 0.0 6 80 1 232 81 0.0 6 81 233 85 0.0 6 83 1 0 1 234 78 0.0 6 77 1 235 72 0.0 6 69 3 236 110 0.0 6 104 6 237 246 0.0 6 242 4 238 123 0.0 6 119 4 239 160 0.0 6 156 4 240 252 0.0 6 251 1 241 151 0.0 6 147 4 242 137 0.0 6 134 3 243 126 0.0 6 123 3 244 125 0.0 6 121 4 245 132 0.0 6 127 5 246 110 0.0 6 105 5 247 231 0.0 6 226 4 1 248 137 0.0 6 135 2 249 236 0.0 6 231 5 250 207 0.0 6 202 5 251 162 0.0 6 159 3 252 422 0.0 6 416 6 253 170 0.0 6 166 4 254 246 0.0 6 240 5 0 0 1 255 259 0.0 6 250 9 256 387 0.0 6 376 10 1 257 408 0.0 6 392 15 1 258 427 0.0 6 417 10 259 524 0.0 6 513 10 0 1 260 332 0.0 6 325 6 1 261 322 0.0 6 317 5 262 198 0.0 6 193 5 263 319 0.0 6 312 7 264 265 0.0 6 259 5 1 265 194 0.0 6 190 4 266 261 0.0 6 249 12 267 329 0.0 6 327 2 268 394 0.0 6 387 7 269 329 0.0 6 320 9 270 180 0.0 6 172 8 271 306 0.0 6 294 12 272 419 0.0 6 410 9 273 549 0.0 6 539 10 274 527 0.0 6 511 15 1 275 536 0.0 6 516 19 1 276 1706 0.0 6 1666 38 1 1 277 3356 0.0 6 3283 72 1 278 12857 0.0 6 12603 250 4 279 135 0.0 6 127 7 0 1 280 8 0.0 6 6 1 0 1 281 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 0 times === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 34722 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 542.7 0 1 11 2 0.0 3 2 12 1 0.0 3 1 13 5 0.0 3 4 0 1 15 1 0.0 4 1 16 2 0.0 4 2 17 4 0.0 5 4 18 2 0.0 5 1 1 19 5 0.0 5 5 20 22 0.0 6 22 21 21 0.0 6 16 5 22 13 0.0 6 11 2 23 11 0.0 6 11 24 3 0.0 6 3 25 10 0.0 6 10 26 1 0.0 6 1 27 2 0.0 6 2 29 3 0.0 6 3 30 3 0.0 6 1 2 31 4 0.0 6 4 32 5 0.0 6 5 33 1 0.0 6 1 34 1 0.0 6 1 36 2 0.0 6 2 37 3 0.0 6 3 38 3 0.0 6 3 39 3 0.0 6 3 40 2 0.0 6 2 41 3 0.0 6 3 42 1 0.0 6 1 43 1 0.0 6 0 1 44 6 0.0 6 6 46 1 0.0 6 1 47 9 0.0 6 9 48 4 0.0 6 4 49 7 0.0 6 7 50 9 0.0 6 9 51 3 0.0 6 3 52 7 0.0 6 6 1 53 2 0.0 6 2 54 9 0.0 6 9 55 14 0.0 6 13 1 56 5 0.0 6 5 57 5 0.0 6 3 1 1 58 5 0.0 6 5 59 4 0.0 6 4 60 6 0.0 6 6 61 2 0.0 6 1 1 62 2 0.0 6 2 63 1 0.0 6 1 64 7 0.0 6 6 1 65 5 0.0 6 5 66 15 0.0 6 13 2 67 12 0.0 6 11 1 68 14 0.0 6 14 69 77 0.0 6 74 3 70 13 0.0 6 11 2 71 27 0.0 6 26 1 72 11 0.0 6 11 73 4 0.0 6 4 74 4 0.0 6 4 75 4 0.0 6 4 76 8 0.0 6 8 77 11 0.0 6 10 1 78 7 0.0 6 7 79 8 0.0 6 7 1 80 7 0.0 6 6 1 81 3 0.0 6 3 82 9 0.0 6 9 83 12 0.0 6 11 0 1 84 16 0.0 6 15 1 85 27 0.0 6 25 2 86 12 0.0 6 11 1 87 12 0.0 6 12 88 3 0.0 6 3 89 17 0.0 6 16 1 90 26 0.0 6 24 2 91 14 0.0 6 11 3 92 4 0.0 6 4 93 21 0.0 6 20 0 0 0 1 94 32 0.0 6 31 0 0 0 1 95 15 0.0 6 14 1 96 14 0.0 6 13 1 97 21 0.0 6 20 1 98 22 0.0 6 21 1 99 15 0.0 6 13 2 100 12 0.0 6 12 101 16 0.0 6 16 102 25 0.0 6 23 1 1 103 32 0.0 6 31 1 104 33 0.0 6 32 1 105 37 0.0 6 36 1 106 22 0.0 6 20 2 107 34 0.0 6 34 108 16 0.0 6 15 1 109 17 0.0 6 17 110 15 0.0 6 15 111 25 0.0 6 22 3 112 14 0.0 6 14 113 14 0.0 6 14 114 16 0.0 6 16 115 35 0.0 6 34 1 116 28 0.0 6 27 1 117 15 0.0 6 15 118 25 0.0 6 24 0 1 119 36 0.0 6 35 1 120 31 0.0 6 29 2 121 23 0.0 6 22 1 122 27 0.0 6 26 1 123 19 0.0 6 19 124 33 0.0 6 33 125 21 0.0 6 19 2 126 15 0.0 6 15 127 23 0.0 6 18 5 128 17 0.0 6 17 129 29 0.0 6 28 1 130 10 0.0 6 9 1 131 43 0.0 6 40 3 132 49 0.0 6 49 133 39 0.0 6 38 1 134 52 0.0 6 50 2 135 123 0.0 6 121 1 1 136 61 0.0 6 59 2 137 77 0.0 6 74 3 138 27 0.0 6 26 1 139 66 0.0 6 62 4 140 104 0.0 6 102 2 141 82 0.0 6 82 142 123 0.0 6 121 2 143 85 0.0 6 82 3 144 168 0.0 6 166 2 145 91 0.0 6 87 4 146 98 0.0 6 97 1 147 54 0.0 6 48 5 0 0 1 148 76 0.0 6 75 1 149 157 0.0 6 154 3 150 200 0.0 6 191 9 151 93 0.0 6 87 6 152 266 0.0 6 255 8 0 2 1 153 133 0.0 6 129 4 154 167 0.0 6 161 6 155 129 0.0 6 125 4 156 118 0.0 6 114 4 157 171 0.0 6 170 1 158 181 0.0 6 171 10 159 85 0.0 6 84 1 160 185 0.0 6 178 7 161 112 0.0 6 109 3 162 72 0.0 6 71 1 163 149 0.0 6 144 4 1 164 149 0.0 6 146 3 165 200 0.0 6 195 4 0 0 1 166 240 0.0 6 233 7 167 141 0.0 6 134 6 0 0 0 0 1 168 313 0.0 6 303 9 1 169 105 0.0 6 101 4 170 183 0.0 6 181 2 171 264 0.0 6 253 11 172 113 0.0 6 106 4 3 173 181 0.0 6 175 4 1 0 0 1 174 241 0.0 6 235 5 1 175 421 0.0 6 408 13 176 245 0.0 6 236 8 0 0 1 177 437 0.0 6 421 16 178 260 0.0 6 253 7 179 305 0.0 6 292 13 180 319 0.0 6 308 10 0 1 181 328 0.0 6 317 11 182 115 0.0 6 111 4 183 295 0.0 6 290 5 184 114 0.0 6 110 4 185 173 0.0 6 169 4 186 774 0.0 6 756 14 0 1 2 1 187 606 0.0 6 591 14 0 0 1 188 703 0.0 6 681 21 0 0 1 189 567 0.0 6 557 10 190 788 0.0 6 768 20 191 653 0.0 6 635 18 192 434 0.0 6 422 10 1 0 1 193 671 0.0 6 654 15 1 0 1 194 469 0.0 6 461 6 2 195 681 0.0 6 662 17 0 0 2 196 838 0.0 6 818 18 1 0 1 197 327 0.0 6 312 14 1 198 1109 0.0 6 1082 26 1 199 836 0.0 6 816 20 200 594 0.0 6 576 18 201 658 0.0 6 641 16 0 0 1 202 565 0.0 6 549 16 203 448 0.0 6 436 11 0 1 204 557 0.0 6 544 11 1 0 1 205 420 0.0 6 410 9 0 1 206 284 0.0 6 277 7 207 303 0.0 6 297 6 208 500 0.0 6 488 12 209 219 0.0 6 215 4 210 222 0.0 6 214 8 211 437 0.0 6 420 17 212 313 0.0 6 302 10 0 0 1 213 148 0.0 6 142 6 214 259 0.0 6 253 6 215 355 0.0 6 344 10 0 0 1 216 564 0.0 6 548 16 217 326 0.0 6 319 7 218 283 0.0 6 271 12 219 266 0.0 6 259 7 220 208 0.0 6 201 6 0 1 221 242 0.0 6 237 3 2 222 235 0.0 6 233 2 223 289 0.0 6 283 6 224 284 0.0 6 281 3 225 297 0.0 6 286 11 226 312 0.0 6 306 6 227 514 0.0 6 507 7 228 322 0.0 6 311 11 229 540 0.0 6 524 15 0 1 230 905 0.0 6 888 17 231 338 0.0 6 326 12 232 488 0.0 6 475 13 233 182 0.0 6 178 3 1 234 317 0.0 6 311 6 235 114 0.0 6 112 2 236 139 0.0 6 138 1 237 143 0.0 6 140 3 238 72 0.0 6 71 1 239 31 0.0 6 30 1 240 49 0.0 6 48 1 241 86 0.0 6 84 1 0 1 242 85 0.0 6 81 4 243 102 0.0 6 102 244 72 0.0 6 69 3 245 119 0.0 6 118 1 246 124 0.0 6 120 4 247 68 0.0 6 68 248 62 0.0 6 60 1 1 249 94 0.0 6 93 0 0 1 250 51 0.0 6 49 1 0 1 251 118 0.0 6 116 2 252 70 0.0 6 70 253 62 0.0 6 59 3 254 32 0.0 6 32 255 30 0.0 6 30 256 12 0.0 6 12 257 3 0.0 6 3 260 1 0.0 6 1 269 1 0.0 6 1 272 1 0.0 6 1 278 1 0.0 6 0 0 0 1 279 4 0.0 6 4 280 17 0.0 6 17 281 45 0.0 6 44 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_20_83_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_20_310_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_20_83_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_20_310_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.32 s (29 µs/read; 2.08 M reads/minute). === Summary === Total read pairs processed: 45,748 Read 1 with adapter: 45,744 (100.0%) Read 2 with adapter: 45,724 (99.9%) == Read fate breakdown == Pairs that were too short: 1 (0.0%) Pairs discarded as untrimmed: 27 (0.1%) Pairs written (passing filters): 45,720 (99.9%) Total basepairs processed: 22,998,052 bp Read 1: 13,120,262 bp Read 2: 9,877,790 bp Total written (filtered): 2,975,053 bp (12.9%) Read 1: 1,510,980 bp Read 2: 1,464,073 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 45688 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 714.8 0 1 5 1 44.7 1 1 6 1 11.2 1 0 1 9 1 0.2 2 1 15 1 0.0 4 1 18 1 0.0 5 1 20 12 0.0 6 12 21 5 0.0 6 4 1 22 5 0.0 6 5 23 4 0.0 6 4 25 7 0.0 6 7 26 3 0.0 6 3 27 1 0.0 6 1 28 1 0.0 6 1 29 1 0.0 6 1 30 1 0.0 6 1 31 2 0.0 6 2 33 3 0.0 6 3 35 1 0.0 6 1 36 2 0.0 6 1 1 37 1 0.0 6 1 38 2 0.0 6 2 39 2 0.0 6 2 40 1 0.0 6 0 0 1 44 4 0.0 6 4 45 1 0.0 6 1 46 4 0.0 6 4 47 1 0.0 6 1 48 2 0.0 6 2 49 1 0.0 6 1 50 2 0.0 6 2 52 2 0.0 6 2 55 1 0.0 6 1 56 5 0.0 6 3 2 57 8 0.0 6 8 58 1 0.0 6 1 61 1 0.0 6 0 1 62 4 0.0 6 3 1 63 2 0.0 6 2 64 5 0.0 6 5 66 2 0.0 6 1 1 67 1 0.0 6 1 68 1 0.0 6 1 69 2 0.0 6 2 70 1 0.0 6 1 72 2 0.0 6 2 73 1 0.0 6 1 77 3 0.0 6 2 1 78 1 0.0 6 1 81 3 0.0 6 3 82 2 0.0 6 2 83 2 0.0 6 2 84 1 0.0 6 1 85 2 0.0 6 2 86 8 0.0 6 8 87 2 0.0 6 2 89 2 0.0 6 2 91 3 0.0 6 3 92 2 0.0 6 2 93 1 0.0 6 1 94 1 0.0 6 1 96 2 0.0 6 2 97 6 0.0 6 6 98 3 0.0 6 3 99 1 0.0 6 0 1 100 2 0.0 6 2 101 3 0.0 6 2 1 103 2 0.0 6 2 104 2 0.0 6 1 1 105 6 0.0 6 6 106 1 0.0 6 1 107 4 0.0 6 4 108 4 0.0 6 4 109 1 0.0 6 1 110 1 0.0 6 1 111 5 0.0 6 4 1 112 6 0.0 6 6 113 5 0.0 6 4 1 114 1 0.0 6 1 115 3 0.0 6 3 116 2 0.0 6 1 1 117 2 0.0 6 2 118 3 0.0 6 2 1 119 6 0.0 6 5 1 120 2 0.0 6 2 121 14 0.0 6 13 1 122 9 0.0 6 9 123 3 0.0 6 3 124 4 0.0 6 4 125 13 0.0 6 13 126 32 0.0 6 32 127 28 0.0 6 27 1 128 12 0.0 6 11 1 129 7 0.0 6 7 130 5 0.0 6 4 1 131 6 0.0 6 6 132 15 0.0 6 15 133 25 0.0 6 24 1 134 13 0.0 6 13 135 16 0.0 6 15 1 136 26 0.0 6 26 137 10 0.0 6 9 1 138 16 0.0 6 14 2 139 16 0.0 6 14 1 1 140 15 0.0 6 14 1 141 23 0.0 6 22 1 142 21 0.0 6 18 2 1 143 42 0.0 6 42 144 31 0.0 6 29 2 145 31 0.0 6 31 146 58 0.0 6 52 5 1 147 41 0.0 6 40 1 148 48 0.0 6 47 1 149 23 0.0 6 23 150 27 0.0 6 26 0 0 0 1 151 31 0.0 6 30 1 152 30 0.0 6 30 153 30 0.0 6 30 154 32 0.0 6 32 155 38 0.0 6 37 1 156 17 0.0 6 17 157 24 0.0 6 24 158 12 0.0 6 12 159 29 0.0 6 28 1 160 22 0.0 6 22 161 39 0.0 6 38 1 162 54 0.0 6 51 2 0 0 1 163 67 0.0 6 67 164 55 0.0 6 54 1 165 50 0.0 6 49 1 166 27 0.0 6 27 167 19 0.0 6 17 2 168 15 0.0 6 15 169 41 0.0 6 39 2 170 29 0.0 6 29 171 48 0.0 6 48 172 34 0.0 6 33 1 173 60 0.0 6 58 2 174 33 0.0 6 33 175 60 0.0 6 57 3 176 55 0.0 6 52 3 177 57 0.0 6 56 1 178 43 0.0 6 43 179 26 0.0 6 26 180 51 0.0 6 49 2 181 107 0.0 6 103 4 182 88 0.0 6 84 4 183 128 0.0 6 122 6 184 121 0.0 6 117 4 185 104 0.0 6 104 186 69 0.0 6 68 1 187 72 0.0 6 70 2 188 73 0.0 6 70 2 0 1 189 77 0.0 6 75 2 190 103 0.0 6 97 6 191 47 0.0 6 47 192 57 0.0 6 57 193 73 0.0 6 72 1 194 122 0.0 6 119 3 195 150 0.0 6 145 5 196 141 0.0 6 138 3 197 123 0.0 6 119 4 198 134 0.0 6 133 1 199 133 0.0 6 132 1 200 137 0.0 6 132 5 201 165 0.0 6 158 7 202 121 0.0 6 117 4 203 182 0.0 6 175 7 204 147 0.0 6 144 3 205 309 0.0 6 303 6 206 224 0.0 6 218 6 207 190 0.0 6 185 5 208 172 0.0 6 167 5 209 99 0.0 6 96 3 210 96 0.0 6 93 2 1 211 133 0.0 6 132 1 212 134 0.0 6 132 1 1 213 114 0.0 6 112 2 214 135 0.0 6 129 6 215 301 0.0 6 295 6 216 181 0.0 6 177 4 217 160 0.0 6 155 4 1 218 112 0.0 6 109 3 219 158 0.0 6 154 3 1 220 200 0.0 6 194 6 221 103 0.0 6 102 1 222 82 0.0 6 82 223 186 0.0 6 184 1 1 224 293 0.0 6 291 2 225 290 0.0 6 285 5 226 212 0.0 6 207 5 227 148 0.0 6 147 1 228 148 0.0 6 144 3 1 229 171 0.0 6 168 3 230 264 0.0 6 259 5 231 348 0.0 6 342 6 232 215 0.0 6 213 2 233 136 0.0 6 132 4 234 224 0.0 6 216 7 1 235 214 0.0 6 212 2 236 322 0.0 6 321 1 237 294 0.0 6 285 9 238 390 0.0 6 383 7 239 436 0.0 6 425 11 240 395 0.0 6 383 12 241 298 0.0 6 292 6 242 277 0.0 6 273 3 1 243 429 0.0 6 421 8 244 161 0.0 6 156 5 245 225 0.0 6 220 4 1 246 92 0.0 6 88 4 247 365 0.0 6 357 8 248 189 0.0 6 185 3 1 249 296 0.0 6 288 8 250 256 0.0 6 246 9 0 0 1 251 152 0.0 6 147 5 252 458 0.0 6 452 6 253 215 0.0 6 214 1 254 210 0.0 6 206 4 255 186 0.0 6 183 2 1 256 346 0.0 6 332 14 257 314 0.0 6 303 11 258 503 0.0 6 494 9 259 174 0.0 6 159 13 1 1 260 265 0.0 6 256 8 0 1 261 615 0.0 6 603 12 262 221 0.0 6 215 6 263 291 0.0 6 287 4 264 159 0.0 6 152 7 265 224 0.0 6 216 8 266 263 0.0 6 256 7 267 455 0.0 6 442 11 1 1 268 146 0.0 6 139 7 269 324 0.0 6 309 15 270 1021 0.0 6 998 22 1 271 384 0.0 6 367 17 272 802 0.0 6 784 18 273 1041 0.0 6 1020 19 1 1 274 1155 0.0 6 1128 27 275 1153 0.0 6 1138 14 0 1 276 599 0.0 6 587 12 277 828 0.0 6 802 18 6 2 278 17995 0.0 6 17538 451 6 279 355 0.0 6 340 14 0 1 280 4 0.0 6 4 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 56 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 76 1 0.0 6 1 81 2 0.0 6 2 83 2 0.0 6 2 84 1 0.0 6 1 85 1 0.0 6 1 86 1 0.0 6 0 0 0 0 0 0 1 93 4 0.0 6 3 1 94 1 0.0 6 1 99 1 0.0 6 1 101 1 0.0 6 1 102 1 0.0 6 1 106 1 0.0 6 1 113 1 0.0 6 1 117 2 0.0 6 2 118 2 0.0 6 2 119 1 0.0 6 1 121 1 0.0 6 1 123 1 0.0 6 1 126 1 0.0 6 1 128 2 0.0 6 2 134 2 0.0 6 2 135 1 0.0 6 1 142 1 0.0 6 1 144 1 0.0 6 1 145 3 0.0 6 3 149 1 0.0 6 1 150 1 0.0 6 1 156 1 0.0 6 1 164 1 0.0 6 1 167 2 0.0 6 2 168 1 0.0 6 1 176 1 0.0 6 1 190 1 0.0 6 1 194 1 0.0 6 1 195 1 0.0 6 1 199 3 0.0 6 3 205 1 0.0 6 1 209 1 0.0 6 1 228 1 0.0 6 1 251 1 0.0 6 1 272 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 45683 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 714.8 0 1 1 9 1 0.2 2 1 10 6 0.0 3 5 1 11 2 0.0 3 2 13 8 0.0 3 6 2 14 4 0.0 4 4 16 7 0.0 4 6 1 17 6 0.0 5 5 1 18 10 0.0 5 8 2 19 4 0.0 5 3 1 20 49 0.0 6 46 3 21 32 0.0 6 30 2 22 25 0.0 6 23 2 23 11 0.0 6 11 24 11 0.0 6 11 25 11 0.0 6 11 26 11 0.0 6 10 1 27 10 0.0 6 8 2 28 7 0.0 6 7 29 14 0.0 6 12 2 30 16 0.0 6 13 2 0 0 1 31 6 0.0 6 5 1 32 3 0.0 6 3 33 5 0.0 6 5 34 3 0.0 6 3 35 4 0.0 6 4 36 3 0.0 6 3 37 10 0.0 6 10 38 9 0.0 6 9 39 2 0.0 6 2 40 4 0.0 6 4 41 5 0.0 6 4 1 42 3 0.0 6 2 1 43 8 0.0 6 8 44 10 0.0 6 10 45 3 0.0 6 3 46 18 0.0 6 16 2 47 8 0.0 6 7 1 48 11 0.0 6 11 49 6 0.0 6 6 50 13 0.0 6 11 2 51 2 0.0 6 2 52 26 0.0 6 24 2 53 5 0.0 6 4 1 54 10 0.0 6 9 1 55 4 0.0 6 4 56 12 0.0 6 11 1 57 18 0.0 6 16 2 58 11 0.0 6 9 2 59 7 0.0 6 7 60 8 0.0 6 8 61 9 0.0 6 8 1 62 7 0.0 6 6 1 63 8 0.0 6 6 1 0 1 64 5 0.0 6 5 65 4 0.0 6 2 2 66 16 0.0 6 13 3 67 86 0.0 6 81 5 68 22 0.0 6 21 1 69 30 0.0 6 27 2 0 0 1 70 21 0.0 6 19 2 71 19 0.0 6 17 2 72 20 0.0 6 19 1 73 10 0.0 6 9 1 74 8 0.0 6 8 75 9 0.0 6 9 76 11 0.0 6 9 2 77 10 0.0 6 9 1 78 19 0.0 6 17 2 79 18 0.0 6 16 2 80 10 0.0 6 10 81 10 0.0 6 8 2 82 22 0.0 6 19 2 1 83 40 0.0 6 36 4 84 21 0.0 6 20 0 1 85 25 0.0 6 23 2 86 18 0.0 6 17 1 87 34 0.0 6 29 5 88 19 0.0 6 18 1 89 26 0.0 6 23 2 0 0 1 90 19 0.0 6 15 3 1 91 23 0.0 6 22 0 1 92 18 0.0 6 18 93 30 0.0 6 27 3 94 48 0.0 6 44 4 95 33 0.0 6 29 3 1 96 31 0.0 6 29 2 97 18 0.0 6 16 2 98 45 0.0 6 41 4 99 22 0.0 6 21 0 1 100 32 0.0 6 31 1 101 27 0.0 6 23 4 102 41 0.0 6 37 4 103 49 0.0 6 44 5 104 33 0.0 6 30 3 105 38 0.0 6 36 2 106 35 0.0 6 29 6 107 22 0.0 6 21 0 0 0 1 108 18 0.0 6 17 1 109 34 0.0 6 33 1 110 35 0.0 6 34 1 111 38 0.0 6 35 3 112 21 0.0 6 20 1 113 30 0.0 6 28 2 114 57 0.0 6 54 2 1 115 35 0.0 6 31 4 116 48 0.0 6 46 2 117 22 0.0 6 21 1 118 46 0.0 6 41 2 3 119 49 0.0 6 44 5 120 74 0.0 6 68 6 121 58 0.0 6 52 6 122 49 0.0 6 46 3 123 52 0.0 6 47 4 1 124 77 0.0 6 71 6 125 69 0.0 6 63 6 126 52 0.0 6 50 2 127 70 0.0 6 68 2 128 60 0.0 6 57 3 129 36 0.0 6 35 1 130 56 0.0 6 53 2 1 131 123 0.0 6 119 4 132 157 0.0 6 156 1 133 115 0.0 6 111 4 134 173 0.0 6 168 4 0 0 0 0 1 135 224 0.0 6 219 5 136 100 0.0 6 97 3 137 127 0.0 6 123 4 138 65 0.0 6 63 2 139 119 0.0 6 116 3 140 182 0.0 6 173 9 141 187 0.0 6 182 5 142 233 0.0 6 221 12 143 215 0.0 6 208 6 1 144 334 0.0 6 323 8 2 0 1 145 141 0.0 6 136 5 146 158 0.0 6 152 5 1 147 112 0.0 6 110 2 148 174 0.0 6 169 5 149 294 0.0 6 286 6 1 0 1 150 495 0.0 6 484 11 151 165 0.0 6 157 7 0 0 0 1 152 490 0.0 6 474 16 153 258 0.0 6 250 6 1 1 154 362 0.0 6 348 14 155 298 0.0 6 286 12 156 224 0.0 6 218 6 157 375 0.0 6 370 4 0 0 1 158 342 0.0 6 335 6 1 159 185 0.0 6 182 3 160 304 0.0 6 297 6 1 161 203 0.0 6 194 8 1 162 176 0.0 6 168 7 1 163 246 0.0 6 237 9 164 269 0.0 6 261 7 1 165 241 0.0 6 232 9 166 360 0.0 6 355 4 0 0 1 167 162 0.0 6 160 1 0 1 168 531 0.0 6 511 17 3 169 162 0.0 6 156 5 0 1 170 230 0.0 6 219 8 1 0 2 171 452 0.0 6 444 7 1 172 158 0.0 6 156 2 173 227 0.0 6 222 5 174 390 0.0 6 375 14 0 0 0 1 175 529 0.0 6 516 10 3 176 401 0.0 6 391 9 0 0 1 177 339 0.0 6 323 13 2 0 1 178 568 0.0 6 551 16 1 179 360 0.0 6 344 16 180 417 0.0 6 402 14 1 181 499 0.0 6 482 15 0 0 2 182 116 0.0 6 110 5 0 1 183 455 0.0 6 439 16 184 302 0.0 6 298 3 1 185 456 0.0 6 441 14 0 0 1 186 909 0.0 6 884 25 187 950 0.0 6 917 30 2 0 1 188 903 0.0 6 871 29 2 1 189 765 0.0 6 733 27 2 1 2 190 1166 0.0 6 1124 40 1 1 191 1092 0.0 6 1060 31 1 192 638 0.0 6 616 22 193 482 0.0 6 467 14 1 194 860 0.0 6 842 18 195 1174 0.0 6 1131 42 0 0 1 196 1870 0.0 6 1825 43 0 1 1 197 501 0.0 6 486 14 1 198 803 0.0 6 763 40 199 1860 0.0 6 1806 52 1 0 1 200 917 0.0 6 882 35 201 955 0.0 6 936 18 1 202 773 0.0 6 751 21 0 0 1 203 602 0.0 6 586 15 1 204 500 0.0 6 480 19 0 0 1 205 254 0.0 6 250 4 206 213 0.0 6 210 2 1 207 119 0.0 6 114 5 208 290 0.0 6 281 8 1 209 270 0.0 6 266 3 1 210 263 0.0 6 246 16 1 211 146 0.0 6 141 5 212 287 0.0 6 285 2 213 204 0.0 6 201 3 214 717 0.0 6 695 19 3 215 521 0.0 6 511 10 216 1025 0.0 6 1000 24 1 217 282 0.0 6 272 10 218 506 0.0 6 485 19 1 1 219 319 0.0 6 315 4 220 254 0.0 6 248 5 0 0 1 221 976 0.0 6 959 16 0 0 1 222 488 0.0 6 469 17 1 0 1 223 567 0.0 6 552 15 224 129 0.0 6 127 1 1 225 143 0.0 6 142 1 226 88 0.0 6 84 4 227 154 0.0 6 149 5 228 73 0.0 6 70 2 1 229 221 0.0 6 217 4 230 91 0.0 6 90 1 231 164 0.0 6 158 5 1 232 183 0.0 6 174 8 1 233 135 0.0 6 133 2 234 90 0.0 6 89 1 235 58 0.0 6 57 1 236 11 0.0 6 11 237 27 0.0 6 25 2 238 18 0.0 6 18 239 30 0.0 6 30 240 62 0.0 6 60 2 241 143 0.0 6 139 4 242 101 0.0 6 98 3 243 116 0.0 6 114 2 244 94 0.0 6 93 1 245 52 0.0 6 51 1 246 95 0.0 6 92 3 247 23 0.0 6 21 2 248 85 0.0 6 83 2 249 18 0.0 6 18 250 15 0.0 6 15 251 40 0.0 6 38 1 0 1 252 5 0.0 6 5 253 6 0.0 6 6 254 2 0.0 6 2 256 2 0.0 6 2 257 1 0.0 6 1 258 1 0.0 6 1 259 1 0.0 6 1 260 2 0.0 6 2 277 3 0.0 6 3 278 52 0.0 6 51 1 279 57 0.0 6 55 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 41 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 2 0.0 6 2 75 1 0.0 6 1 80 1 0.0 6 1 82 1 0.0 6 1 83 1 0.0 6 1 85 1 0.0 6 1 91 1 0.0 6 1 92 2 0.0 6 2 98 4 0.0 6 4 103 1 0.0 6 1 105 3 0.0 6 3 106 1 0.0 6 1 107 1 0.0 6 1 109 1 0.0 6 1 110 1 0.0 6 1 111 1 0.0 6 1 114 1 0.0 6 1 115 1 0.0 6 1 116 2 0.0 6 2 127 1 0.0 6 1 134 1 0.0 6 1 145 1 0.0 6 1 150 1 0.0 6 1 153 1 0.0 6 1 167 1 0.0 6 1 174 1 0.0 6 1 176 1 0.0 6 1 177 1 0.0 6 1 178 1 0.0 6 1 188 1 0.0 6 1 201 1 0.0 6 1 207 1 0.0 6 1 228 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_01_72_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_01_299_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_01_72_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_01_299_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_02_73_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_02_300_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_02_73_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_02_300_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_03_74_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_03_301_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_03_74_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_03_301_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_04_75_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_04_302_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_04_75_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_04_302_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_05_76_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_05_303_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_05_76_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_05_303_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_06_77_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_06_304_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_06_77_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_06_304_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_15_78_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_15_305_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_15_78_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_15_305_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_16_79_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_16_306_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_16_79_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_16_306_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_17_80_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_17_307_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_17_80_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_17_307_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_18_81_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_18_308_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_18_81_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_18_308_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_19_82_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_19_309_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_19_82_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_19_309_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_20_83_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_07-Rev_20_310_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_20_83_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_07-Rev_20_310_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_01_84_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_01_311_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_01_84_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_01_311_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.79 s (31 µs/read; 1.94 M reads/minute). === Summary === Total read pairs processed: 58,018 Read 1 with adapter: 58,018 (100.0%) Read 2 with adapter: 57,967 (99.9%) == Read fate breakdown == Pairs that were too short: 22 (0.0%) Pairs discarded as untrimmed: 51 (0.1%) Pairs written (passing filters): 57,945 (99.9%) Total basepairs processed: 25,189,058 bp Read 1: 14,541,856 bp Read 2: 10,647,202 bp Total written (filtered): 3,531,429 bp (14.0%) Read 1: 1,778,461 bp Read 2: 1,752,968 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 56964 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 2 14.2 1 2 7 1 3.5 2 1 8 2 0.9 2 1 1 10 1 0.1 3 0 1 12 1 0.0 3 1 15 1 0.0 4 1 16 3 0.0 4 2 1 17 4 0.0 5 4 19 2 0.0 5 1 1 20 101 0.0 6 98 3 21 53 0.0 6 53 22 55 0.0 6 54 1 23 25 0.0 6 23 2 24 25 0.0 6 25 25 14 0.0 6 13 0 0 1 26 12 0.0 6 12 27 11 0.0 6 10 1 28 14 0.0 6 14 29 11 0.0 6 11 30 9 0.0 6 9 31 9 0.0 6 9 32 7 0.0 6 6 1 33 11 0.0 6 11 34 5 0.0 6 5 35 5 0.0 6 5 36 4 0.0 6 4 37 3 0.0 6 3 38 4 0.0 6 4 39 6 0.0 6 6 40 11 0.0 6 10 0 0 1 41 8 0.0 6 8 42 2 0.0 6 2 43 7 0.0 6 5 2 44 8 0.0 6 8 45 4 0.0 6 4 46 2 0.0 6 2 47 5 0.0 6 5 48 6 0.0 6 6 49 6 0.0 6 6 50 2 0.0 6 2 51 14 0.0 6 14 52 6 0.0 6 6 53 12 0.0 6 12 54 2 0.0 6 2 55 3 0.0 6 3 56 5 0.0 6 5 57 7 0.0 6 7 58 2 0.0 6 2 59 6 0.0 6 6 60 1 0.0 6 1 61 3 0.0 6 2 1 62 2 0.0 6 2 63 8 0.0 6 7 1 64 9 0.0 6 9 65 3 0.0 6 3 66 7 0.0 6 7 67 10 0.0 6 9 1 68 6 0.0 6 6 69 7 0.0 6 6 0 0 0 1 70 9 0.0 6 9 71 9 0.0 6 9 72 11 0.0 6 11 73 4 0.0 6 4 74 6 0.0 6 5 1 75 5 0.0 6 5 76 16 0.0 6 16 77 7 0.0 6 7 78 2 0.0 6 2 79 5 0.0 6 5 80 7 0.0 6 7 81 5 0.0 6 5 82 9 0.0 6 9 83 11 0.0 6 11 84 14 0.0 6 14 85 9 0.0 6 9 86 10 0.0 6 10 87 9 0.0 6 7 2 88 8 0.0 6 6 2 89 9 0.0 6 9 90 9 0.0 6 9 91 8 0.0 6 8 92 7 0.0 6 4 2 0 0 0 0 1 93 5 0.0 6 4 1 94 9 0.0 6 9 95 3 0.0 6 3 96 13 0.0 6 13 97 6 0.0 6 6 98 8 0.0 6 7 1 99 7 0.0 6 7 100 12 0.0 6 11 0 1 101 6 0.0 6 6 102 12 0.0 6 12 103 13 0.0 6 13 104 15 0.0 6 15 105 10 0.0 6 10 106 11 0.0 6 10 1 107 12 0.0 6 11 1 108 14 0.0 6 13 0 0 0 0 0 1 109 18 0.0 6 18 110 12 0.0 6 12 111 7 0.0 6 7 112 8 0.0 6 8 113 23 0.0 6 23 114 11 0.0 6 10 1 115 24 0.0 6 23 1 116 17 0.0 6 17 117 13 0.0 6 11 1 0 0 0 0 1 118 16 0.0 6 15 1 119 16 0.0 6 16 120 16 0.0 6 16 121 24 0.0 6 22 2 122 22 0.0 6 22 123 16 0.0 6 16 124 13 0.0 6 12 1 125 19 0.0 6 18 0 1 126 29 0.0 6 28 1 127 25 0.0 6 23 2 128 24 0.0 6 22 2 129 10 0.0 6 9 1 130 20 0.0 6 20 131 19 0.0 6 19 132 26 0.0 6 25 1 133 26 0.0 6 26 134 22 0.0 6 20 2 135 33 0.0 6 32 1 136 28 0.0 6 28 137 23 0.0 6 22 1 138 29 0.0 6 28 1 139 34 0.0 6 32 2 140 43 0.0 6 42 1 141 66 0.0 6 65 1 142 171 0.0 6 170 1 143 201 0.0 6 199 2 144 117 0.0 6 116 1 145 145 0.0 6 145 146 183 0.0 6 180 2 0 0 0 1 147 149 0.0 6 147 2 148 144 0.0 6 142 1 1 149 158 0.0 6 158 150 184 0.0 6 182 2 151 199 0.0 6 196 2 0 0 0 1 152 139 0.0 6 138 1 153 123 0.0 6 121 2 154 146 0.0 6 146 155 134 0.0 6 132 2 156 143 0.0 6 141 2 157 133 0.0 6 130 3 158 169 0.0 6 166 3 159 140 0.0 6 136 3 1 160 148 0.0 6 143 5 161 158 0.0 6 157 1 162 151 0.0 6 150 1 163 129 0.0 6 125 4 164 171 0.0 6 169 2 165 152 0.0 6 152 166 147 0.0 6 145 2 167 201 0.0 6 197 4 168 136 0.0 6 136 169 165 0.0 6 164 1 170 175 0.0 6 172 2 0 1 171 169 0.0 6 165 2 2 172 171 0.0 6 170 1 173 202 0.0 6 201 0 1 174 154 0.0 6 153 1 175 157 0.0 6 157 176 189 0.0 6 186 2 0 1 177 217 0.0 6 216 1 178 241 0.0 6 238 3 179 505 0.0 6 502 3 180 225 0.0 6 220 5 181 209 0.0 6 207 2 182 192 0.0 6 190 2 183 174 0.0 6 171 3 184 193 0.0 6 191 2 185 223 0.0 6 220 2 0 0 0 0 1 186 243 0.0 6 240 2 0 0 0 1 187 221 0.0 6 217 4 188 260 0.0 6 256 4 189 252 0.0 6 245 6 0 1 190 225 0.0 6 220 5 191 259 0.0 6 256 3 192 261 0.0 6 253 6 2 193 286 0.0 6 280 6 194 359 0.0 6 356 3 195 371 0.0 6 365 5 0 0 0 0 1 196 406 0.0 6 400 5 1 197 501 0.0 6 492 7 1 0 1 198 552 0.0 6 546 3 1 1 1 199 583 0.0 6 573 8 1 0 0 0 1 200 633 0.0 6 623 7 0 0 1 0 2 201 575 0.0 6 573 1 1 202 471 0.0 6 465 4 0 0 1 0 1 203 540 0.0 6 534 5 0 0 1 204 479 0.0 6 473 6 205 463 0.0 6 459 4 206 511 0.0 6 503 8 207 545 0.0 6 539 4 2 208 709 0.0 6 705 3 0 1 209 559 0.0 6 552 7 210 658 0.0 6 647 10 1 211 480 0.0 6 474 6 212 490 0.0 6 485 4 0 0 0 1 213 644 0.0 6 634 9 1 214 681 0.0 6 672 9 215 724 0.0 6 716 7 0 0 1 216 763 0.0 6 757 5 1 217 862 0.0 6 849 12 0 0 0 1 218 653 0.0 6 646 6 0 0 0 0 1 219 674 0.0 6 670 4 220 735 0.0 6 727 7 0 0 0 0 1 221 669 0.0 6 662 5 1 1 222 573 0.0 6 569 4 223 536 0.0 6 532 4 224 531 0.0 6 526 4 0 0 0 1 225 623 0.0 6 620 3 226 543 0.0 6 533 7 0 0 0 2 1 227 682 0.0 6 677 5 228 672 0.0 6 665 6 1 229 635 0.0 6 632 1 1 0 1 230 577 0.0 6 569 7 0 0 0 1 231 558 0.0 6 553 5 232 578 0.0 6 573 5 233 535 0.0 6 525 8 0 0 0 1 1 234 601 0.0 6 593 6 1 0 0 1 235 604 0.0 6 596 4 1 1 0 1 1 236 544 0.0 6 540 4 237 563 0.0 6 560 3 238 550 0.0 6 548 2 239 563 0.0 6 558 5 240 537 0.0 6 526 8 0 1 0 0 2 241 515 0.0 6 511 4 242 469 0.0 6 464 5 243 539 0.0 6 535 3 1 244 502 0.0 6 497 5 245 471 0.0 6 468 2 0 0 1 246 428 0.0 6 423 4 0 1 247 407 0.0 6 404 3 248 362 0.0 6 359 3 249 359 0.0 6 356 3 250 360 0.0 6 356 4 251 350 0.0 6 350 252 385 0.0 6 379 6 253 382 0.0 6 378 1 1 0 2 254 344 0.0 6 340 3 1 255 379 0.0 6 372 5 0 2 256 314 0.0 6 313 1 257 348 0.0 6 347 1 258 323 0.0 6 322 1 259 343 0.0 6 341 2 260 332 0.0 6 327 5 261 394 0.0 6 393 1 262 314 0.0 6 310 4 263 239 0.0 6 236 3 264 284 0.0 6 282 1 0 0 1 265 244 0.0 6 243 1 266 258 0.0 6 257 1 267 257 0.0 6 255 2 268 261 0.0 6 259 2 269 253 0.0 6 253 270 284 0.0 6 283 0 0 0 1 271 289 0.0 6 288 1 272 268 0.0 6 266 2 273 218 0.0 6 215 3 274 245 0.0 6 240 5 275 286 0.0 6 284 2 276 345 0.0 6 343 2 277 410 0.0 6 406 3 1 278 428 0.0 6 422 5 1 279 615 0.0 6 611 4 280 1986 0.0 6 1971 14 0 0 0 0 1 281 2476 0.0 6 2451 25 282 12 0.0 6 12 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1054 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 7 226.6 1 7 61 5 0.0 6 5 69 1 0.0 6 1 70 4 0.0 6 4 71 3 0.0 6 3 72 7 0.0 6 7 73 5 0.0 6 5 74 3 0.0 6 3 75 5 0.0 6 5 76 3 0.0 6 3 77 6 0.0 6 6 78 4 0.0 6 4 79 3 0.0 6 3 80 7 0.0 6 7 81 7 0.0 6 7 82 3 0.0 6 3 83 5 0.0 6 5 84 4 0.0 6 4 85 1 0.0 6 1 86 5 0.0 6 5 87 7 0.0 6 7 88 2 0.0 6 2 89 4 0.0 6 4 90 1 0.0 6 1 91 3 0.0 6 3 92 2 0.0 6 2 93 4 0.0 6 4 94 2 0.0 6 2 95 3 0.0 6 3 96 2 0.0 6 2 97 1 0.0 6 1 98 1 0.0 6 1 99 3 0.0 6 3 100 2 0.0 6 2 101 3 0.0 6 3 102 2 0.0 6 2 103 1 0.0 6 1 104 5 0.0 6 5 105 1 0.0 6 1 106 2 0.0 6 2 107 5 0.0 6 5 108 16 0.0 6 16 109 2 0.0 6 2 110 2 0.0 6 2 111 4 0.0 6 4 112 4 0.0 6 4 113 1 0.0 6 1 115 5 0.0 6 5 116 1 0.0 6 1 117 2 0.0 6 2 118 3 0.0 6 3 119 1 0.0 6 1 120 6 0.0 6 6 121 1 0.0 6 1 122 6 0.0 6 6 123 4 0.0 6 4 124 3 0.0 6 3 125 1 0.0 6 1 126 7 0.0 6 7 127 3 0.0 6 3 128 2 0.0 6 2 129 3 0.0 6 3 130 6 0.0 6 6 131 4 0.0 6 4 132 10 0.0 6 9 1 133 4 0.0 6 4 134 3 0.0 6 3 135 8 0.0 6 8 136 6 0.0 6 6 137 9 0.0 6 9 138 3 0.0 6 3 139 7 0.0 6 7 140 1 0.0 6 1 141 2 0.0 6 2 142 4 0.0 6 4 143 2 0.0 6 2 144 2 0.0 6 2 145 5 0.0 6 5 146 2 0.0 6 2 147 5 0.0 6 5 148 2 0.0 6 2 149 3 0.0 6 3 150 6 0.0 6 6 151 5 0.0 6 5 152 4 0.0 6 4 153 5 0.0 6 5 154 8 0.0 6 8 155 5 0.0 6 5 156 6 0.0 6 6 157 4 0.0 6 4 158 5 0.0 6 5 159 4 0.0 6 3 1 160 3 0.0 6 3 161 5 0.0 6 5 162 5 0.0 6 5 163 4 0.0 6 4 164 9 0.0 6 9 165 10 0.0 6 10 166 5 0.0 6 5 167 5 0.0 6 5 168 8 0.0 6 8 169 7 0.0 6 7 170 10 0.0 6 10 171 7 0.0 6 7 172 3 0.0 6 3 173 7 0.0 6 7 174 5 0.0 6 5 175 2 0.0 6 2 176 1 0.0 6 1 177 7 0.0 6 7 178 7 0.0 6 7 179 4 0.0 6 4 180 6 0.0 6 5 0 0 0 0 0 1 181 3 0.0 6 3 182 8 0.0 6 8 183 9 0.0 6 9 184 8 0.0 6 8 185 10 0.0 6 10 186 9 0.0 6 9 187 9 0.0 6 9 188 4 0.0 6 4 189 11 0.0 6 11 190 5 0.0 6 5 191 4 0.0 6 4 192 7 0.0 6 7 193 5 0.0 6 5 194 9 0.0 6 9 195 9 0.0 6 9 196 4 0.0 6 4 197 4 0.0 6 4 198 11 0.0 6 11 199 5 0.0 6 5 200 9 0.0 6 9 201 7 0.0 6 7 202 4 0.0 6 3 0 0 0 0 0 1 203 6 0.0 6 6 204 5 0.0 6 5 205 6 0.0 6 6 206 5 0.0 6 5 207 5 0.0 6 5 208 7 0.0 6 7 209 7 0.0 6 7 210 6 0.0 6 6 211 6 0.0 6 6 212 8 0.0 6 8 213 7 0.0 6 7 214 2 0.0 6 2 215 3 0.0 6 3 216 4 0.0 6 4 217 2 0.0 6 2 218 5 0.0 6 5 219 8 0.0 6 8 220 10 0.0 6 10 221 5 0.0 6 5 222 4 0.0 6 4 223 3 0.0 6 3 225 9 0.0 6 9 226 10 0.0 6 10 227 15 0.0 6 15 228 7 0.0 6 7 229 6 0.0 6 6 230 5 0.0 6 5 231 5 0.0 6 5 232 6 0.0 6 6 233 9 0.0 6 9 234 5 0.0 6 5 235 10 0.0 6 10 236 5 0.0 6 5 237 6 0.0 6 6 238 3 0.0 6 3 239 16 0.0 6 16 240 7 0.0 6 7 241 4 0.0 6 4 242 3 0.0 6 3 243 6 0.0 6 6 244 7 0.0 6 7 245 7 0.0 6 7 246 8 0.0 6 8 247 8 0.0 6 8 248 4 0.0 6 4 249 6 0.0 6 6 250 4 0.0 6 4 251 8 0.0 6 8 252 4 0.0 6 4 253 4 0.0 6 4 254 4 0.0 6 4 255 2 0.0 6 2 256 5 0.0 6 5 257 4 0.0 6 4 258 6 0.0 6 6 259 4 0.0 6 4 260 6 0.0 6 6 261 3 0.0 6 3 262 2 0.0 6 2 263 1 0.0 6 1 264 2 0.0 6 2 265 3 0.0 6 3 266 7 0.0 6 6 1 267 7 0.0 6 7 268 2 0.0 6 2 269 2 0.0 6 2 270 1 0.0 6 1 271 4 0.0 6 4 272 1 0.0 6 1 273 1 0.0 6 1 274 2 0.0 6 2 275 2 0.0 6 2 277 6 0.0 6 6 279 2 0.0 6 2 280 8 0.0 6 8 281 4 0.0 6 4 282 3 0.0 6 3 284 1 0.0 6 1 285 2 0.0 6 2 286 2 0.0 6 2 287 1 0.0 6 1 289 1 0.0 6 1 290 1 0.0 6 1 291 1 0.0 6 1 300 1 0.0 6 1 308 1 0.0 6 1 309 1 0.0 6 1 310 1 0.0 6 1 311 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 57393 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 226.6 1 0 1 7 1 3.5 2 0 1 9 1 0.2 2 1 11 2 0.0 3 2 12 4 0.0 3 4 13 16 0.0 3 16 14 10 0.0 4 10 16 10 0.0 4 10 17 31 0.0 5 29 1 1 18 19 0.0 5 18 1 19 20 0.0 5 17 3 20 112 0.0 6 109 3 21 93 0.0 6 91 2 22 46 0.0 6 46 23 51 0.0 6 48 3 24 34 0.0 6 33 1 25 26 0.0 6 25 0 1 26 24 0.0 6 20 4 27 32 0.0 6 30 1 1 28 22 0.0 6 21 1 29 24 0.0 6 22 2 30 29 0.0 6 29 31 19 0.0 6 18 1 32 25 0.0 6 22 3 33 13 0.0 6 10 3 34 18 0.0 6 14 3 1 35 17 0.0 6 14 0 2 0 0 1 36 15 0.0 6 14 1 37 16 0.0 6 15 1 38 22 0.0 6 18 2 1 0 0 0 1 39 10 0.0 6 10 40 16 0.0 6 13 3 41 19 0.0 6 17 2 42 14 0.0 6 13 1 43 21 0.0 6 20 1 44 16 0.0 6 16 45 24 0.0 6 21 2 0 0 0 0 1 46 23 0.0 6 21 2 47 29 0.0 6 26 3 48 22 0.0 6 21 1 49 22 0.0 6 21 1 50 27 0.0 6 25 2 51 21 0.0 6 20 1 52 34 0.0 6 34 53 32 0.0 6 31 1 54 23 0.0 6 20 3 55 28 0.0 6 26 1 1 56 38 0.0 6 35 3 57 23 0.0 6 21 1 0 0 0 0 1 58 25 0.0 6 24 1 59 36 0.0 6 33 2 1 60 37 0.0 6 34 3 61 38 0.0 6 34 3 1 62 28 0.0 6 27 1 63 27 0.0 6 25 0 2 64 29 0.0 6 25 4 65 39 0.0 6 36 2 0 1 66 36 0.0 6 34 1 1 67 42 0.0 6 37 4 0 0 1 68 60 0.0 6 56 3 1 69 121 0.0 6 116 4 0 1 70 49 0.0 6 45 2 0 0 2 71 68 0.0 6 65 3 72 50 0.0 6 48 1 0 0 0 0 1 73 58 0.0 6 56 2 74 43 0.0 6 41 2 75 40 0.0 6 36 4 76 50 0.0 6 49 0 0 0 1 77 49 0.0 6 48 1 78 45 0.0 6 42 2 0 0 1 79 52 0.0 6 51 1 80 58 0.0 6 55 3 81 65 0.0 6 64 1 82 70 0.0 6 64 5 0 0 1 83 57 0.0 6 54 3 84 63 0.0 6 60 2 0 1 85 84 0.0 6 80 3 1 86 59 0.0 6 53 6 87 50 0.0 6 46 4 88 55 0.0 6 54 1 89 81 0.0 6 77 3 1 90 53 0.0 6 50 2 0 1 91 76 0.0 6 73 2 0 0 0 0 1 92 71 0.0 6 68 3 93 65 0.0 6 60 5 94 80 0.0 6 78 2 95 92 0.0 6 91 0 0 0 1 96 89 0.0 6 87 2 97 117 0.0 6 114 3 98 141 0.0 6 136 5 99 105 0.0 6 98 5 1 1 100 88 0.0 6 84 3 0 0 0 0 1 101 96 0.0 6 92 3 1 102 84 0.0 6 81 2 0 1 103 128 0.0 6 126 2 104 125 0.0 6 122 3 105 138 0.0 6 138 106 207 0.0 6 206 1 107 155 0.0 6 151 4 108 129 0.0 6 127 2 109 152 0.0 6 150 2 110 130 0.0 6 125 4 1 111 123 0.0 6 121 2 112 152 0.0 6 143 6 0 0 1 2 113 165 0.0 6 163 1 0 0 0 1 114 192 0.0 6 184 5 1 0 1 1 115 184 0.0 6 180 3 0 0 0 1 116 176 0.0 6 173 3 117 169 0.0 6 164 3 0 1 1 118 185 0.0 6 180 4 0 1 119 215 0.0 6 205 10 120 223 0.0 6 215 6 1 0 0 1 121 206 0.0 6 196 9 0 0 0 0 1 122 216 0.0 6 209 7 123 207 0.0 6 197 7 0 1 2 124 223 0.0 6 217 5 0 0 0 1 125 238 0.0 6 231 4 1 0 2 126 272 0.0 6 263 5 1 0 2 1 127 307 0.0 6 299 5 1 0 2 128 293 0.0 6 290 2 0 1 129 303 0.0 6 293 8 2 130 360 0.0 6 351 7 2 131 401 0.0 6 387 13 0 0 0 1 132 371 0.0 6 361 8 1 0 1 133 455 0.0 6 440 13 1 0 0 1 134 465 0.0 6 442 16 2 0 2 2 1 135 446 0.0 6 429 11 2 1 1 2 136 532 0.0 6 518 12 0 0 1 1 137 545 0.0 6 534 6 1 1 0 2 1 138 488 0.0 6 474 12 0 0 1 1 139 556 0.0 6 543 13 140 583 0.0 6 561 17 0 2 0 3 141 576 0.0 6 561 13 0 0 0 1 1 142 674 0.0 6 661 11 1 1 143 704 0.0 6 685 17 0 1 1 144 711 0.0 6 691 16 0 2 0 1 1 145 727 0.0 6 705 21 0 0 0 0 1 146 838 0.0 6 824 10 0 0 1 0 3 147 784 0.0 6 762 16 2 0 1 0 3 148 957 0.0 6 936 13 0 2 2 2 2 149 925 0.0 6 911 10 2 1 1 150 980 0.0 6 951 23 2 2 0 1 1 151 1086 0.0 6 1059 24 1 0 1 0 1 152 1429 0.0 6 1399 21 4 3 2 153 1051 0.0 6 1025 18 2 1 1 3 1 154 1317 0.0 6 1289 23 0 4 1 155 1244 0.0 6 1216 24 2 1 1 156 1042 0.0 6 1021 16 0 2 1 0 2 157 1097 0.0 6 1072 15 0 4 2 2 2 158 939 0.0 6 907 26 4 0 2 159 947 0.0 6 921 23 0 0 2 0 1 160 1027 0.0 6 1009 10 1 0 2 2 3 161 849 0.0 6 825 22 0 0 0 1 1 162 853 0.0 6 837 14 1 1 163 730 0.0 6 710 15 1 1 0 1 2 164 679 0.0 6 664 11 1 2 0 0 1 165 680 0.0 6 668 11 0 0 0 0 1 166 679 0.0 6 664 11 2 1 0 0 1 167 618 0.0 6 606 12 168 817 0.0 6 808 5 2 0 1 0 1 169 638 0.0 6 621 14 0 2 0 0 1 170 800 0.0 6 784 11 1 0 3 1 171 640 0.0 6 628 11 0 0 1 172 648 0.0 6 634 8 1 2 1 0 2 173 722 0.0 6 709 10 0 0 3 174 795 0.0 6 783 8 1 0 1 1 1 175 721 0.0 6 715 5 0 0 1 176 653 0.0 6 636 14 1 1 0 1 177 612 0.0 6 599 13 178 644 0.0 6 634 7 2 1 179 512 0.0 6 504 6 1 0 0 0 1 180 495 0.0 6 489 5 0 0 1 181 393 0.0 6 388 5 182 375 0.0 6 367 4 3 1 183 379 0.0 6 371 7 1 184 375 0.0 6 366 5 2 0 0 1 1 185 583 0.0 6 571 9 2 0 0 0 1 186 690 0.0 6 683 7 187 620 0.0 6 603 14 1 0 0 1 1 188 441 0.0 6 436 5 189 373 0.0 6 366 5 0 0 0 2 190 362 0.0 6 359 2 1 191 352 0.0 6 346 4 1 1 192 301 0.0 6 293 7 1 193 335 0.0 6 331 2 2 194 298 0.0 6 291 6 1 195 284 0.0 6 279 3 0 1 1 196 252 0.0 6 245 6 1 197 194 0.0 6 191 3 198 287 0.0 6 283 4 199 264 0.0 6 263 1 200 207 0.0 6 202 5 201 190 0.0 6 188 1 0 0 0 0 1 202 161 0.0 6 157 3 0 1 203 130 0.0 6 127 3 204 158 0.0 6 152 3 0 0 0 3 205 146 0.0 6 144 2 206 127 0.0 6 125 2 207 96 0.0 6 94 2 208 111 0.0 6 107 4 209 97 0.0 6 96 1 210 87 0.0 6 85 2 211 88 0.0 6 83 5 212 81 0.0 6 77 4 213 68 0.0 6 67 1 214 85 0.0 6 85 215 70 0.0 6 70 216 106 0.0 6 105 1 217 82 0.0 6 82 218 64 0.0 6 62 2 219 51 0.0 6 47 4 220 51 0.0 6 50 0 1 221 43 0.0 6 43 222 37 0.0 6 37 223 38 0.0 6 37 1 224 50 0.0 6 49 1 225 46 0.0 6 46 226 30 0.0 6 30 227 43 0.0 6 42 1 228 29 0.0 6 28 1 229 37 0.0 6 35 2 230 29 0.0 6 28 1 231 17 0.0 6 16 1 232 31 0.0 6 30 1 233 22 0.0 6 21 1 234 20 0.0 6 20 235 11 0.0 6 11 236 17 0.0 6 16 1 237 11 0.0 6 11 238 7 0.0 6 7 239 3 0.0 6 3 240 7 0.0 6 7 241 7 0.0 6 7 242 4 0.0 6 4 243 12 0.0 6 12 244 4 0.0 6 4 245 4 0.0 6 4 246 3 0.0 6 3 247 3 0.0 6 3 248 6 0.0 6 6 249 8 0.0 6 8 250 7 0.0 6 6 0 1 251 3 0.0 6 3 252 2 0.0 6 2 253 1 0.0 6 1 254 2 0.0 6 2 255 3 0.0 6 3 279 2 0.0 6 2 280 10 0.0 6 10 281 99 0.0 6 98 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 574 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 54 1 0.0 6 1 57 1 0.0 6 1 59 1 0.0 6 1 61 1 0.0 6 1 63 2 0.0 6 2 64 1 0.0 6 1 69 1 0.0 6 1 70 4 0.0 6 4 71 2 0.0 6 2 72 6 0.0 6 6 73 3 0.0 6 3 74 8 0.0 6 8 75 9 0.0 6 8 1 76 7 0.0 6 6 0 0 0 1 77 8 0.0 6 8 78 7 0.0 6 7 79 9 0.0 6 9 80 14 0.0 6 14 81 4 0.0 6 4 82 2 0.0 6 2 83 4 0.0 6 4 84 4 0.0 6 4 85 1 0.0 6 1 86 5 0.0 6 5 88 2 0.0 6 2 89 5 0.0 6 5 90 3 0.0 6 3 91 1 0.0 6 1 92 5 0.0 6 5 93 6 0.0 6 6 94 2 0.0 6 2 95 2 0.0 6 2 96 4 0.0 6 4 97 2 0.0 6 2 98 4 0.0 6 4 100 3 0.0 6 3 101 1 0.0 6 1 102 7 0.0 6 7 103 2 0.0 6 2 104 3 0.0 6 3 105 4 0.0 6 4 106 5 0.0 6 5 107 3 0.0 6 3 108 14 0.0 6 14 109 1 0.0 6 0 0 0 1 110 2 0.0 6 2 111 3 0.0 6 3 112 1 0.0 6 1 113 3 0.0 6 2 0 0 0 0 0 1 114 4 0.0 6 4 115 4 0.0 6 4 116 3 0.0 6 3 117 5 0.0 6 5 118 6 0.0 6 6 119 2 0.0 6 2 120 3 0.0 6 3 121 7 0.0 6 7 122 3 0.0 6 3 123 3 0.0 6 3 124 2 0.0 6 2 125 1 0.0 6 1 126 7 0.0 6 7 127 1 0.0 6 1 129 6 0.0 6 6 130 6 0.0 6 6 131 8 0.0 6 8 132 7 0.0 6 7 133 5 0.0 6 5 134 3 0.0 6 3 135 2 0.0 6 2 136 2 0.0 6 2 137 5 0.0 6 5 138 4 0.0 6 4 139 3 0.0 6 3 140 5 0.0 6 5 141 2 0.0 6 2 142 4 0.0 6 4 143 1 0.0 6 1 144 3 0.0 6 2 1 146 7 0.0 6 7 147 3 0.0 6 3 148 6 0.0 6 6 149 7 0.0 6 7 150 10 0.0 6 10 151 4 0.0 6 4 152 1 0.0 6 1 153 3 0.0 6 3 154 4 0.0 6 4 155 4 0.0 6 4 156 2 0.0 6 2 157 3 0.0 6 3 158 2 0.0 6 2 159 4 0.0 6 4 160 4 0.0 6 4 161 3 0.0 6 3 162 4 0.0 6 4 163 5 0.0 6 5 164 2 0.0 6 2 165 6 0.0 6 6 166 6 0.0 6 6 167 6 0.0 6 6 168 9 0.0 6 9 169 4 0.0 6 4 170 4 0.0 6 4 171 6 0.0 6 6 172 8 0.0 6 7 0 0 0 0 1 173 7 0.0 6 7 174 5 0.0 6 5 175 5 0.0 6 5 176 4 0.0 6 4 177 4 0.0 6 4 178 2 0.0 6 1 1 179 5 0.0 6 4 0 0 0 1 180 3 0.0 6 3 181 6 0.0 6 6 182 5 0.0 6 5 183 11 0.0 6 11 184 6 0.0 6 6 185 5 0.0 6 5 186 2 0.0 6 2 187 6 0.0 6 6 188 1 0.0 6 1 189 4 0.0 6 4 190 4 0.0 6 4 191 3 0.0 6 3 192 1 0.0 6 0 1 193 2 0.0 6 2 194 4 0.0 6 4 195 1 0.0 6 1 196 2 0.0 6 2 197 3 0.0 6 3 198 4 0.0 6 4 199 1 0.0 6 1 200 1 0.0 6 1 201 2 0.0 6 2 202 4 0.0 6 4 203 1 0.0 6 1 206 1 0.0 6 1 207 2 0.0 6 2 208 2 0.0 6 2 210 1 0.0 6 1 212 2 0.0 6 2 213 2 0.0 6 2 214 1 0.0 6 0 0 0 0 1 215 1 0.0 6 0 1 216 1 0.0 6 1 217 2 0.0 6 2 218 1 0.0 6 1 220 1 0.0 6 1 222 1 0.0 6 1 223 1 0.0 6 1 224 1 0.0 6 1 226 1 0.0 6 1 229 1 0.0 6 1 232 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_02_85_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_02_312_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_02_85_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_02_312_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.87 s (27 µs/read; 2.19 M reads/minute). === Summary === Total read pairs processed: 68,325 Read 1 with adapter: 68,325 (100.0%) Read 2 with adapter: 68,325 (100.0%) == Read fate breakdown == Pairs that were too short: 21 (0.0%) Pairs discarded as untrimmed: 0 (0.0%) Pairs written (passing filters): 68,304 (100.0%) Total basepairs processed: 30,143,578 bp Read 1: 17,084,978 bp Read 2: 13,058,600 bp Total written (filtered): 4,238,563 bp (14.1%) Read 1: 2,099,558 bp Read 2: 2,139,005 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 67128 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 2 66.7 1 2 7 3 4.2 2 3 9 5 0.3 2 3 1 0 1 11 2 0.0 3 1 1 12 1 0.0 3 1 15 2 0.0 4 2 19 1 0.0 5 1 20 100 0.0 6 95 5 21 71 0.0 6 68 3 22 55 0.0 6 53 2 23 37 0.0 6 35 2 24 21 0.0 6 21 25 23 0.0 6 23 26 17 0.0 6 16 1 27 17 0.0 6 17 28 13 0.0 6 12 1 29 11 0.0 6 11 30 8 0.0 6 8 31 15 0.0 6 15 32 5 0.0 6 5 33 11 0.0 6 11 34 8 0.0 6 8 35 5 0.0 6 5 36 6 0.0 6 6 37 7 0.0 6 7 38 3 0.0 6 3 39 12 0.0 6 12 40 10 0.0 6 9 1 41 10 0.0 6 10 42 8 0.0 6 8 43 11 0.0 6 11 44 10 0.0 6 10 45 4 0.0 6 4 46 7 0.0 6 6 1 48 5 0.0 6 5 49 8 0.0 6 8 50 6 0.0 6 6 51 7 0.0 6 6 0 0 0 0 0 1 52 9 0.0 6 9 53 5 0.0 6 5 54 5 0.0 6 4 1 55 12 0.0 6 12 56 5 0.0 6 5 57 7 0.0 6 7 58 3 0.0 6 3 59 8 0.0 6 7 1 60 1 0.0 6 1 61 3 0.0 6 3 62 10 0.0 6 10 63 8 0.0 6 8 64 4 0.0 6 4 65 6 0.0 6 6 66 4 0.0 6 4 67 7 0.0 6 6 1 68 9 0.0 6 9 69 12 0.0 6 10 2 70 11 0.0 6 11 71 10 0.0 6 10 72 9 0.0 6 8 1 73 9 0.0 6 7 1 0 1 74 9 0.0 6 8 1 75 9 0.0 6 8 1 76 18 0.0 6 18 77 10 0.0 6 10 78 8 0.0 6 8 79 8 0.0 6 7 1 80 7 0.0 6 7 81 10 0.0 6 10 82 11 0.0 6 11 83 11 0.0 6 11 84 11 0.0 6 11 85 10 0.0 6 10 86 21 0.0 6 21 87 15 0.0 6 14 1 88 9 0.0 6 8 0 0 1 89 6 0.0 6 4 0 1 0 0 1 90 10 0.0 6 9 1 91 15 0.0 6 15 92 7 0.0 6 5 2 93 7 0.0 6 6 1 94 4 0.0 6 4 95 14 0.0 6 14 96 7 0.0 6 7 97 13 0.0 6 13 98 9 0.0 6 8 1 99 11 0.0 6 10 1 100 11 0.0 6 11 101 4 0.0 6 4 102 20 0.0 6 19 1 103 13 0.0 6 11 2 104 23 0.0 6 19 4 105 14 0.0 6 12 2 106 19 0.0 6 18 1 107 21 0.0 6 21 108 16 0.0 6 16 109 21 0.0 6 21 110 19 0.0 6 18 0 0 0 0 1 111 15 0.0 6 15 112 22 0.0 6 21 1 113 15 0.0 6 15 114 14 0.0 6 14 115 13 0.0 6 13 116 12 0.0 6 12 117 25 0.0 6 23 2 118 14 0.0 6 13 1 119 16 0.0 6 16 120 25 0.0 6 24 0 1 121 25 0.0 6 24 1 122 21 0.0 6 21 123 27 0.0 6 27 124 21 0.0 6 19 2 125 31 0.0 6 28 3 126 32 0.0 6 32 127 30 0.0 6 29 1 128 25 0.0 6 23 2 129 22 0.0 6 20 2 130 30 0.0 6 29 1 131 34 0.0 6 32 2 132 28 0.0 6 27 1 133 41 0.0 6 40 1 134 30 0.0 6 29 1 135 28 0.0 6 28 136 41 0.0 6 39 2 137 41 0.0 6 40 1 138 38 0.0 6 38 139 37 0.0 6 37 140 46 0.0 6 46 141 46 0.0 6 46 142 149 0.0 6 147 1 1 143 250 0.0 6 249 0 1 144 360 0.0 6 359 1 145 217 0.0 6 216 1 146 223 0.0 6 223 147 296 0.0 6 294 2 148 271 0.0 6 270 1 149 195 0.0 6 194 1 150 266 0.0 6 265 1 151 218 0.0 6 218 152 255 0.0 6 254 1 153 205 0.0 6 204 0 0 1 154 165 0.0 6 164 1 155 179 0.0 6 178 1 156 182 0.0 6 180 2 157 201 0.0 6 200 1 158 168 0.0 6 168 159 180 0.0 6 179 1 160 215 0.0 6 213 1 0 0 1 161 204 0.0 6 203 1 162 245 0.0 6 243 1 0 1 163 218 0.0 6 215 2 0 0 1 164 227 0.0 6 226 1 165 227 0.0 6 227 166 199 0.0 6 198 1 167 200 0.0 6 198 2 168 209 0.0 6 205 3 0 0 0 1 169 222 0.0 6 220 2 170 239 0.0 6 236 3 171 242 0.0 6 240 2 172 190 0.0 6 189 0 0 0 0 0 1 173 218 0.0 6 213 5 174 252 0.0 6 252 175 244 0.0 6 243 1 176 231 0.0 6 230 1 177 266 0.0 6 264 1 0 1 178 287 0.0 6 285 2 179 259 0.0 6 258 1 180 580 0.0 6 578 2 181 309 0.0 6 305 4 182 265 0.0 6 263 2 183 242 0.0 6 242 184 224 0.0 6 221 2 0 1 185 284 0.0 6 282 2 186 315 0.0 6 313 2 187 351 0.0 6 347 4 188 354 0.0 6 347 6 0 0 0 1 189 357 0.0 6 355 2 190 318 0.0 6 316 2 191 324 0.0 6 321 3 192 375 0.0 6 371 4 193 365 0.0 6 361 4 194 466 0.0 6 460 5 0 0 0 0 1 195 478 0.0 6 476 2 196 522 0.0 6 519 2 1 197 553 0.0 6 549 3 0 1 198 758 0.0 6 752 5 0 1 199 651 0.0 6 643 3 1 1 1 1 1 200 729 0.0 6 722 7 201 565 0.0 6 564 1 202 568 0.0 6 564 3 0 0 0 1 203 625 0.0 6 619 6 204 621 0.0 6 617 4 205 602 0.0 6 598 3 0 1 206 601 0.0 6 599 2 207 684 0.0 6 679 4 0 0 0 0 1 208 852 0.0 6 840 11 0 0 0 0 1 209 681 0.0 6 679 2 210 758 0.0 6 749 8 0 0 0 1 211 598 0.0 6 597 1 212 602 0.0 6 595 5 2 213 786 0.0 6 782 4 214 727 0.0 6 720 6 0 0 0 0 1 215 726 0.0 6 724 2 216 911 0.0 6 903 6 0 1 1 217 970 0.0 6 963 6 1 218 773 0.0 6 769 3 1 219 790 0.0 6 782 8 220 774 0.0 6 766 8 221 711 0.0 6 706 4 0 1 222 631 0.0 6 625 5 0 1 223 648 0.0 6 644 2 0 2 224 637 0.0 6 635 2 225 630 0.0 6 628 2 226 712 0.0 6 709 2 0 0 0 0 1 227 801 0.0 6 797 4 228 786 0.0 6 783 3 229 626 0.0 6 621 4 0 1 230 548 0.0 6 545 3 231 621 0.0 6 616 5 232 586 0.0 6 581 3 1 0 0 1 233 631 0.0 6 627 4 234 578 0.0 6 574 4 235 596 0.0 6 593 1 0 1 1 236 487 0.0 6 486 1 237 479 0.0 6 478 1 238 463 0.0 6 462 1 239 477 0.0 6 474 3 240 486 0.0 6 480 6 241 520 0.0 6 517 2 1 242 469 0.0 6 466 3 243 427 0.0 6 424 2 1 244 464 0.0 6 455 9 245 428 0.0 6 423 4 1 246 325 0.0 6 322 3 247 335 0.0 6 331 4 248 277 0.0 6 277 249 313 0.0 6 310 3 250 302 0.0 6 295 7 251 300 0.0 6 297 2 0 0 1 252 292 0.0 6 287 3 2 253 256 0.0 6 255 1 254 265 0.0 6 261 4 255 255 0.0 6 253 1 0 1 256 269 0.0 6 267 2 257 221 0.0 6 219 2 258 259 0.0 6 252 6 0 0 0 0 1 259 269 0.0 6 262 7 260 273 0.0 6 270 3 261 241 0.0 6 236 2 2 0 1 262 245 0.0 6 242 3 263 224 0.0 6 218 5 1 264 182 0.0 6 178 3 1 265 165 0.0 6 163 2 266 161 0.0 6 158 3 267 178 0.0 6 175 3 268 181 0.0 6 175 6 269 160 0.0 6 158 2 270 150 0.0 6 149 1 271 208 0.0 6 198 10 272 220 0.0 6 218 2 273 164 0.0 6 159 5 274 171 0.0 6 167 3 1 275 160 0.0 6 158 2 276 292 0.0 6 283 7 2 277 497 0.0 6 486 11 278 356 0.0 6 347 8 1 279 523 0.0 6 513 10 280 1058 0.0 6 1017 38 2 0 0 1 281 9309 0.0 6 9136 172 1 282 19 0.0 6 17 1 1 283 2 0.0 6 2 284 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1197 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 16 266.9 1 16 57 1 0.0 6 0 0 0 0 1 61 35 0.0 6 34 1 70 4 0.0 6 4 71 11 0.0 6 11 72 16 0.0 6 16 73 5 0.0 6 5 74 5 0.0 6 5 75 8 0.0 6 8 76 4 0.0 6 4 77 4 0.0 6 4 78 9 0.0 6 9 79 3 0.0 6 3 80 4 0.0 6 4 81 2 0.0 6 2 82 6 0.0 6 6 83 4 0.0 6 4 84 5 0.0 6 5 85 2 0.0 6 2 86 2 0.0 6 2 87 4 0.0 6 4 88 2 0.0 6 2 89 4 0.0 6 4 90 11 0.0 6 11 91 6 0.0 6 6 92 1 0.0 6 1 93 5 0.0 6 5 94 1 0.0 6 1 95 6 0.0 6 6 96 4 0.0 6 4 97 4 0.0 6 4 98 3 0.0 6 3 99 3 0.0 6 3 100 3 0.0 6 3 101 3 0.0 6 3 102 5 0.0 6 5 103 3 0.0 6 3 104 5 0.0 6 5 105 1 0.0 6 1 106 3 0.0 6 3 107 13 0.0 6 13 108 14 0.0 6 14 109 6 0.0 6 6 110 7 0.0 6 7 111 2 0.0 6 2 112 4 0.0 6 4 113 7 0.0 6 7 114 3 0.0 6 3 115 5 0.0 6 5 116 2 0.0 6 2 117 1 0.0 6 1 118 1 0.0 6 1 119 3 0.0 6 3 120 1 0.0 6 1 121 5 0.0 6 5 122 6 0.0 6 6 123 7 0.0 6 7 124 3 0.0 6 3 125 7 0.0 6 7 126 6 0.0 6 6 127 4 0.0 6 4 128 2 0.0 6 2 129 1 0.0 6 1 130 5 0.0 6 5 131 11 0.0 6 11 132 5 0.0 6 5 133 3 0.0 6 3 134 5 0.0 6 5 135 8 0.0 6 8 136 4 0.0 6 4 137 6 0.0 6 6 138 11 0.0 6 11 139 9 0.0 6 9 140 7 0.0 6 7 141 9 0.0 6 9 142 6 0.0 6 6 143 3 0.0 6 3 144 6 0.0 6 6 145 8 0.0 6 8 146 6 0.0 6 6 147 6 0.0 6 6 148 5 0.0 6 5 149 4 0.0 6 4 150 4 0.0 6 4 151 10 0.0 6 10 152 4 0.0 6 4 153 3 0.0 6 3 154 9 0.0 6 9 155 10 0.0 6 10 156 6 0.0 6 6 157 7 0.0 6 7 158 11 0.0 6 11 159 11 0.0 6 11 160 8 0.0 6 8 161 8 0.0 6 8 162 5 0.0 6 5 163 5 0.0 6 5 164 11 0.0 6 11 165 12 0.0 6 12 166 4 0.0 6 4 167 7 0.0 6 7 168 12 0.0 6 12 169 8 0.0 6 8 170 6 0.0 6 6 171 7 0.0 6 7 172 9 0.0 6 9 173 6 0.0 6 6 174 6 0.0 6 6 175 7 0.0 6 7 176 6 0.0 6 6 177 13 0.0 6 13 178 7 0.0 6 7 179 10 0.0 6 10 180 8 0.0 6 8 181 8 0.0 6 8 182 6 0.0 6 6 183 7 0.0 6 7 184 10 0.0 6 10 185 9 0.0 6 9 186 14 0.0 6 14 187 14 0.0 6 14 188 9 0.0 6 9 189 9 0.0 6 9 190 13 0.0 6 13 191 12 0.0 6 12 192 12 0.0 6 12 193 5 0.0 6 5 194 10 0.0 6 10 195 6 0.0 6 6 196 11 0.0 6 11 197 7 0.0 6 7 198 7 0.0 6 7 199 8 0.0 6 8 200 10 0.0 6 10 201 9 0.0 6 9 202 2 0.0 6 2 203 5 0.0 6 5 204 10 0.0 6 10 205 7 0.0 6 7 206 2 0.0 6 2 207 4 0.0 6 4 208 4 0.0 6 4 209 4 0.0 6 4 210 8 0.0 6 8 211 7 0.0 6 7 212 6 0.0 6 6 213 4 0.0 6 4 214 4 0.0 6 4 215 6 0.0 6 6 216 4 0.0 6 4 217 6 0.0 6 6 218 4 0.0 6 4 219 7 0.0 6 7 220 8 0.0 6 8 221 6 0.0 6 6 222 5 0.0 6 5 223 7 0.0 6 7 224 7 0.0 6 7 225 6 0.0 6 6 226 8 0.0 6 8 227 6 0.0 6 6 228 8 0.0 6 8 229 5 0.0 6 5 230 13 0.0 6 13 231 5 0.0 6 5 232 8 0.0 6 8 233 5 0.0 6 5 234 3 0.0 6 3 235 7 0.0 6 7 236 3 0.0 6 3 237 3 0.0 6 3 238 1 0.0 6 1 239 2 0.0 6 2 240 6 0.0 6 6 241 8 0.0 6 8 242 3 0.0 6 3 243 3 0.0 6 3 244 3 0.0 6 3 245 5 0.0 6 5 246 5 0.0 6 5 247 2 0.0 6 2 248 3 0.0 6 3 249 3 0.0 6 3 250 4 0.0 6 4 251 4 0.0 6 4 252 3 0.0 6 3 254 2 0.0 6 2 255 1 0.0 6 1 256 2 0.0 6 2 259 2 0.0 6 2 260 1 0.0 6 1 261 1 0.0 6 1 262 1 0.0 6 1 263 4 0.0 6 4 264 3 0.0 6 3 265 1 0.0 6 1 266 2 0.0 6 2 267 1 0.0 6 1 268 3 0.0 6 3 271 1 0.0 6 1 272 1 0.0 6 1 274 1 0.0 6 1 276 3 0.0 6 3 277 1 0.0 6 1 278 1 0.0 6 1 281 1 0.0 6 1 282 1 0.0 6 1 283 1 0.0 6 1 285 1 0.0 6 1 290 1 0.0 6 1 300 1 0.0 6 1 302 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 67321 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1067.6 0 2 5 2 66.7 1 1 1 6 1 16.7 1 1 9 1 0.3 2 1 10 3 0.1 3 3 11 5 0.0 3 5 12 13 0.0 3 13 13 6 0.0 3 5 1 14 11 0.0 4 11 15 2 0.0 4 2 16 4 0.0 4 4 17 49 0.0 5 47 2 18 26 0.0 5 24 2 19 27 0.0 5 25 2 20 74 0.0 6 70 4 21 58 0.0 6 58 22 35 0.0 6 33 1 1 23 22 0.0 6 21 1 24 21 0.0 6 19 2 25 17 0.0 6 15 2 26 18 0.0 6 14 4 27 19 0.0 6 17 1 1 28 10 0.0 6 10 29 12 0.0 6 12 30 12 0.0 6 11 1 31 25 0.0 6 20 5 32 16 0.0 6 15 1 33 12 0.0 6 11 1 34 10 0.0 6 10 35 11 0.0 6 10 1 36 17 0.0 6 16 1 37 23 0.0 6 20 2 1 38 15 0.0 6 11 4 39 13 0.0 6 12 1 40 14 0.0 6 13 1 41 10 0.0 6 9 1 42 17 0.0 6 16 0 1 43 13 0.0 6 13 44 19 0.0 6 18 1 45 19 0.0 6 16 2 1 46 23 0.0 6 21 2 47 19 0.0 6 18 1 48 18 0.0 6 18 49 24 0.0 6 24 50 29 0.0 6 28 1 51 15 0.0 6 13 1 1 52 22 0.0 6 18 3 1 53 28 0.0 6 25 3 54 32 0.0 6 29 3 55 22 0.0 6 20 2 56 27 0.0 6 24 3 57 24 0.0 6 24 58 38 0.0 6 36 2 59 32 0.0 6 30 2 60 31 0.0 6 26 3 1 1 61 35 0.0 6 32 3 62 24 0.0 6 21 3 63 24 0.0 6 23 1 64 37 0.0 6 34 3 65 27 0.0 6 25 2 66 41 0.0 6 38 3 67 44 0.0 6 40 4 68 131 0.0 6 127 4 69 63 0.0 6 60 2 1 70 86 0.0 6 84 2 71 44 0.0 6 41 2 0 0 1 72 69 0.0 6 66 2 1 73 52 0.0 6 49 3 74 35 0.0 6 34 1 75 45 0.0 6 44 1 76 43 0.0 6 42 1 77 42 0.0 6 41 1 78 51 0.0 6 50 1 79 46 0.0 6 44 2 80 64 0.0 6 60 4 81 66 0.0 6 60 3 0 0 1 1 1 82 63 0.0 6 61 2 83 60 0.0 6 58 2 84 95 0.0 6 89 4 1 0 1 85 49 0.0 6 43 6 86 56 0.0 6 53 2 0 0 0 0 1 87 53 0.0 6 49 4 88 46 0.0 6 45 1 89 64 0.0 6 60 2 0 0 1 0 1 90 68 0.0 6 67 0 0 1 91 73 0.0 6 70 2 0 1 92 64 0.0 6 59 4 0 0 1 93 75 0.0 6 70 5 94 88 0.0 6 83 4 1 95 72 0.0 6 67 5 96 70 0.0 6 69 1 97 87 0.0 6 84 3 98 85 0.0 6 81 3 1 99 75 0.0 6 74 1 100 80 0.0 6 77 1 1 0 0 1 101 97 0.0 6 93 4 102 126 0.0 6 121 4 0 0 1 103 101 0.0 6 100 1 104 139 0.0 6 136 3 105 186 0.0 6 178 7 0 0 1 106 113 0.0 6 108 4 1 107 108 0.0 6 104 4 108 118 0.0 6 111 6 1 109 126 0.0 6 120 4 0 0 1 0 1 110 107 0.0 6 105 2 111 149 0.0 6 140 6 1 0 0 1 1 112 140 0.0 6 135 2 1 0 1 0 1 113 152 0.0 6 148 2 1 0 0 0 1 114 138 0.0 6 132 3 1 1 1 115 147 0.0 6 145 2 116 156 0.0 6 151 5 117 148 0.0 6 146 2 118 164 0.0 6 158 3 2 1 119 219 0.0 6 216 2 0 0 0 1 120 206 0.0 6 198 5 2 0 1 121 180 0.0 6 175 5 122 204 0.0 6 195 6 1 1 0 0 1 123 179 0.0 6 175 4 124 219 0.0 6 211 6 0 1 0 1 125 257 0.0 6 254 3 126 271 0.0 6 265 6 127 288 0.0 6 279 9 128 278 0.0 6 267 9 1 0 0 0 1 129 308 0.0 6 302 6 130 339 0.0 6 333 5 0 0 0 1 131 362 0.0 6 356 4 2 132 367 0.0 6 359 7 0 1 133 418 0.0 6 401 15 1 0 1 134 428 0.0 6 422 5 0 1 135 466 0.0 6 458 7 0 0 0 1 136 463 0.0 6 449 13 1 137 444 0.0 6 436 8 138 507 0.0 6 498 9 139 562 0.0 6 553 7 1 0 0 0 1 140 724 0.0 6 713 8 0 1 1 1 141 684 0.0 6 678 4 0 0 1 0 1 142 711 0.0 6 698 11 1 0 0 1 143 812 0.0 6 803 8 0 0 0 0 1 144 839 0.0 6 825 12 0 0 1 0 1 145 690 0.0 6 678 9 1 1 0 0 1 146 805 0.0 6 791 13 0 0 1 147 839 0.0 6 825 10 1 1 1 0 1 148 828 0.0 6 808 18 1 0 0 1 149 929 0.0 6 911 16 0 0 0 1 1 150 1068 0.0 6 1050 16 0 1 0 0 1 151 1348 0.0 6 1323 19 1 1 1 2 1 152 1137 0.0 6 1108 25 1 0 0 1 2 153 1284 0.0 6 1267 15 0 0 1 1 154 1210 0.0 6 1191 15 2 0 1 0 1 155 974 0.0 6 962 11 1 156 1079 0.0 6 1061 16 0 0 0 1 1 157 964 0.0 6 945 16 2 0 0 1 158 1148 0.0 6 1133 12 0 0 1 2 159 998 0.0 6 983 10 1 0 1 1 2 160 954 0.0 6 945 9 161 877 0.0 6 867 7 0 1 1 0 1 162 807 0.0 6 794 9 1 0 0 2 1 163 830 0.0 6 808 15 3 2 1 1 164 830 0.0 6 819 10 0 0 0 0 1 165 790 0.0 6 777 12 0 0 0 1 166 785 0.0 6 769 13 0 2 0 0 1 167 892 0.0 6 881 10 0 0 0 0 1 168 802 0.0 6 785 15 0 1 1 169 873 0.0 6 859 12 0 0 0 0 2 170 775 0.0 6 762 10 1 1 0 0 1 171 774 0.0 6 756 14 2 1 1 172 791 0.0 6 781 6 1 0 1 1 1 173 911 0.0 6 891 16 0 1 2 0 1 174 916 0.0 6 902 12 1 0 1 175 959 0.0 6 940 15 4 176 852 0.0 6 842 10 177 855 0.0 6 845 8 2 178 656 0.0 6 651 5 179 746 0.0 6 734 8 1 0 1 2 180 547 0.0 6 540 6 0 0 1 181 615 0.0 6 601 13 1 182 485 0.0 6 480 5 183 687 0.0 6 678 9 184 726 0.0 6 718 7 0 0 1 185 848 0.0 6 837 10 1 186 954 0.0 6 942 12 187 730 0.0 6 717 10 1 1 0 1 188 647 0.0 6 636 11 189 609 0.0 6 600 7 0 2 190 818 0.0 6 800 14 0 0 3 0 1 191 660 0.0 6 646 13 1 192 665 0.0 6 653 11 1 193 453 0.0 6 444 8 1 194 482 0.0 6 473 8 0 1 195 491 0.0 6 479 12 196 759 0.0 6 740 19 197 556 0.0 6 547 9 198 663 0.0 6 650 10 1 0 1 0 1 199 714 0.0 6 701 13 200 388 0.0 6 381 6 0 0 0 0 1 201 470 0.0 6 461 8 1 202 270 0.0 6 265 5 203 238 0.0 6 230 8 204 323 0.0 6 315 8 205 182 0.0 6 178 4 206 195 0.0 6 193 2 207 154 0.0 6 152 2 208 180 0.0 6 174 6 209 164 0.0 6 164 210 150 0.0 6 149 0 1 211 171 0.0 6 166 4 1 212 189 0.0 6 185 4 213 122 0.0 6 120 2 214 145 0.0 6 139 6 215 184 0.0 6 176 7 1 216 141 0.0 6 139 2 217 169 0.0 6 165 4 218 120 0.0 6 119 1 219 167 0.0 6 164 2 0 1 220 61 0.0 6 60 1 221 88 0.0 6 85 2 0 0 1 222 101 0.0 6 97 4 223 114 0.0 6 109 4 1 224 71 0.0 6 68 3 225 97 0.0 6 95 2 226 78 0.0 6 77 1 227 84 0.0 6 82 2 228 72 0.0 6 71 1 229 113 0.0 6 111 2 230 55 0.0 6 54 1 231 43 0.0 6 42 1 232 90 0.0 6 84 5 1 233 91 0.0 6 91 234 22 0.0 6 22 235 16 0.0 6 15 1 236 7 0.0 6 7 237 15 0.0 6 15 238 14 0.0 6 14 239 7 0.0 6 7 240 20 0.0 6 19 1 241 22 0.0 6 21 1 242 28 0.0 6 27 1 243 37 0.0 6 35 2 244 21 0.0 6 21 245 6 0.0 6 5 1 246 13 0.0 6 13 247 7 0.0 6 7 248 14 0.0 6 14 249 9 0.0 6 9 250 1 0.0 6 1 251 4 0.0 6 4 252 3 0.0 6 3 253 3 0.0 6 3 254 3 0.0 6 3 255 4 0.0 6 4 257 2 0.0 6 2 277 1 0.0 6 1 279 8 0.0 6 8 280 56 0.0 6 56 281 3 0.0 6 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1004 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 3 90 1067.6 0 90 52 2 0.0 6 2 55 2 0.0 6 2 60 1 0.0 6 1 62 8 0.0 6 8 71 7 0.0 6 7 72 18 0.0 6 18 73 11 0.0 6 11 74 8 0.0 6 8 75 9 0.0 6 9 76 6 0.0 6 6 77 13 0.0 6 13 78 9 0.0 6 9 79 4 0.0 6 4 80 3 0.0 6 3 81 17 0.0 6 17 82 6 0.0 6 6 83 4 0.0 6 4 84 10 0.0 6 10 85 2 0.0 6 2 86 6 0.0 6 6 87 7 0.0 6 7 88 4 0.0 6 4 89 5 0.0 6 5 90 3 0.0 6 3 91 7 0.0 6 7 92 6 0.0 6 6 93 5 0.0 6 5 94 10 0.0 6 10 95 5 0.0 6 5 96 5 0.0 6 5 97 5 0.0 6 5 98 3 0.0 6 3 99 1 0.0 6 1 100 7 0.0 6 7 101 2 0.0 6 2 102 3 0.0 6 3 103 5 0.0 6 5 104 5 0.0 6 5 105 3 0.0 6 3 106 8 0.0 6 8 107 4 0.0 6 4 108 9 0.0 6 9 109 17 0.0 6 17 110 5 0.0 6 5 111 5 0.0 6 4 0 0 0 1 112 8 0.0 6 8 113 4 0.0 6 4 114 4 0.0 6 3 0 0 0 1 115 1 0.0 6 1 116 8 0.0 6 8 117 4 0.0 6 4 118 4 0.0 6 4 119 3 0.0 6 3 120 2 0.0 6 2 121 2 0.0 6 2 122 4 0.0 6 4 123 8 0.0 6 8 124 7 0.0 6 7 125 7 0.0 6 7 126 2 0.0 6 2 127 9 0.0 6 9 128 3 0.0 6 3 129 4 0.0 6 4 130 3 0.0 6 3 131 5 0.0 6 5 132 10 0.0 6 10 133 12 0.0 6 12 134 3 0.0 6 3 135 6 0.0 6 6 136 5 0.0 6 5 137 6 0.0 6 6 138 11 0.0 6 11 139 11 0.0 6 11 140 9 0.0 6 9 141 7 0.0 6 7 142 15 0.0 6 15 143 9 0.0 6 8 1 144 4 0.0 6 4 145 5 0.0 6 5 146 8 0.0 6 8 147 8 0.0 6 8 148 11 0.0 6 11 149 7 0.0 6 7 150 9 0.0 6 9 151 4 0.0 6 4 152 5 0.0 6 5 153 9 0.0 6 9 154 9 0.0 6 9 155 5 0.0 6 5 156 10 0.0 6 10 157 9 0.0 6 9 158 11 0.0 6 11 159 14 0.0 6 14 160 3 0.0 6 3 161 6 0.0 6 6 162 10 0.0 6 10 163 7 0.0 6 7 164 11 0.0 6 11 165 10 0.0 6 9 0 0 0 1 166 5 0.0 6 5 167 6 0.0 6 6 168 10 0.0 6 10 169 13 0.0 6 13 170 13 0.0 6 13 171 7 0.0 6 7 172 5 0.0 6 5 173 9 0.0 6 9 174 8 0.0 6 8 175 10 0.0 6 10 176 6 0.0 6 6 177 3 0.0 6 3 178 5 0.0 6 5 179 3 0.0 6 3 180 4 0.0 6 4 181 8 0.0 6 8 182 3 0.0 6 3 183 4 0.0 6 4 184 3 0.0 6 3 185 9 0.0 6 9 186 2 0.0 6 2 187 7 0.0 6 7 188 6 0.0 6 6 189 6 0.0 6 6 190 8 0.0 6 8 191 5 0.0 6 5 192 7 0.0 6 7 193 4 0.0 6 4 194 4 0.0 6 4 195 2 0.0 6 2 196 4 0.0 6 4 197 12 0.0 6 12 198 3 0.0 6 3 199 6 0.0 6 6 200 4 0.0 6 4 201 2 0.0 6 2 203 4 0.0 6 4 204 1 0.0 6 1 205 3 0.0 6 3 206 2 0.0 6 2 207 3 0.0 6 3 208 1 0.0 6 1 209 2 0.0 6 2 210 4 0.0 6 4 211 3 0.0 6 3 212 1 0.0 6 1 213 4 0.0 6 4 214 1 0.0 6 0 0 0 0 1 216 3 0.0 6 3 217 1 0.0 6 1 219 1 0.0 6 1 220 1 0.0 6 1 221 1 0.0 6 1 224 1 0.0 6 1 227 2 0.0 6 2 228 1 0.0 6 1 229 1 0.0 6 1 239 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_03_86_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_03_313_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_03_86_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_03_313_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.68 s (29 µs/read; 2.10 M reads/minute). === Summary === Total read pairs processed: 93,882 Read 1 with adapter: 93,882 (100.0%) Read 2 with adapter: 93,808 (99.9%) == Read fate breakdown == Pairs that were too short: 21 (0.0%) Pairs discarded as untrimmed: 74 (0.1%) Pairs written (passing filters): 93,787 (99.9%) Total basepairs processed: 44,207,094 bp Read 1: 24,981,822 bp Read 2: 19,225,272 bp Total written (filtered): 5,874,885 bp (13.3%) Read 1: 2,858,766 bp Read 2: 3,016,119 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 92969 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 2 91.7 1 2 6 2 22.9 1 1 0 1 7 2 5.7 2 0 0 2 8 1 1.4 2 1 9 1 0.4 2 1 10 2 0.1 3 2 11 2 0.0 3 2 13 1 0.0 3 0 1 16 2 0.0 4 2 17 1 0.0 5 0 1 18 1 0.0 5 1 19 2 0.0 5 2 20 113 0.0 6 110 3 21 73 0.0 6 71 2 22 51 0.0 6 51 23 33 0.0 6 31 2 24 20 0.0 6 20 25 21 0.0 6 21 26 19 0.0 6 19 27 7 0.0 6 7 28 14 0.0 6 14 29 11 0.0 6 10 1 30 8 0.0 6 7 1 31 5 0.0 6 5 32 5 0.0 6 5 33 8 0.0 6 6 2 34 6 0.0 6 6 35 5 0.0 6 4 1 36 6 0.0 6 6 37 7 0.0 6 7 38 4 0.0 6 4 39 6 0.0 6 6 40 8 0.0 6 8 41 10 0.0 6 9 0 0 0 1 42 8 0.0 6 8 43 9 0.0 6 9 44 11 0.0 6 11 45 11 0.0 6 11 46 6 0.0 6 5 1 47 1 0.0 6 1 48 6 0.0 6 6 49 3 0.0 6 3 50 2 0.0 6 1 1 51 5 0.0 6 4 0 0 1 52 12 0.0 6 12 53 4 0.0 6 4 54 6 0.0 6 5 1 55 1 0.0 6 1 56 5 0.0 6 4 1 57 8 0.0 6 8 58 4 0.0 6 3 1 59 4 0.0 6 4 60 5 0.0 6 3 2 61 3 0.0 6 2 1 62 4 0.0 6 3 0 0 0 0 0 1 63 2 0.0 6 1 1 64 4 0.0 6 4 65 3 0.0 6 3 66 7 0.0 6 6 1 67 6 0.0 6 6 68 2 0.0 6 2 69 5 0.0 6 5 70 6 0.0 6 6 71 5 0.0 6 4 1 72 6 0.0 6 5 1 73 5 0.0 6 5 74 12 0.0 6 11 1 75 5 0.0 6 5 76 6 0.0 6 6 77 12 0.0 6 10 2 78 10 0.0 6 9 1 79 6 0.0 6 5 1 80 6 0.0 6 6 81 4 0.0 6 4 82 3 0.0 6 3 83 8 0.0 6 7 1 84 7 0.0 6 7 85 7 0.0 6 7 86 12 0.0 6 12 87 11 0.0 6 10 1 88 9 0.0 6 8 1 89 11 0.0 6 11 90 9 0.0 6 8 1 91 9 0.0 6 8 1 92 10 0.0 6 8 2 93 9 0.0 6 8 0 0 0 0 1 94 11 0.0 6 11 95 3 0.0 6 2 1 96 7 0.0 6 7 97 11 0.0 6 11 98 10 0.0 6 9 1 99 10 0.0 6 10 100 11 0.0 6 11 101 11 0.0 6 11 102 17 0.0 6 14 2 1 103 13 0.0 6 13 104 15 0.0 6 14 1 105 13 0.0 6 13 106 22 0.0 6 22 107 21 0.0 6 20 0 1 108 19 0.0 6 18 1 109 13 0.0 6 11 2 110 6 0.0 6 6 111 18 0.0 6 18 112 20 0.0 6 19 1 113 14 0.0 6 14 114 15 0.0 6 14 0 1 115 13 0.0 6 13 116 17 0.0 6 16 1 117 15 0.0 6 14 1 118 21 0.0 6 21 119 16 0.0 6 16 120 11 0.0 6 11 121 22 0.0 6 22 122 31 0.0 6 31 123 25 0.0 6 23 2 124 21 0.0 6 20 1 125 25 0.0 6 25 126 34 0.0 6 34 127 29 0.0 6 29 128 32 0.0 6 30 2 129 29 0.0 6 29 130 16 0.0 6 15 1 131 24 0.0 6 24 132 40 0.0 6 37 2 1 133 19 0.0 6 18 1 134 33 0.0 6 30 3 135 34 0.0 6 34 136 28 0.0 6 28 137 45 0.0 6 43 2 138 32 0.0 6 32 139 41 0.0 6 40 1 140 56 0.0 6 55 1 141 59 0.0 6 58 1 142 60 0.0 6 60 143 130 0.0 6 127 3 144 226 0.0 6 226 145 314 0.0 6 314 146 190 0.0 6 188 2 147 197 0.0 6 194 3 148 245 0.0 6 243 2 149 202 0.0 6 201 1 150 187 0.0 6 187 151 229 0.0 6 228 1 152 199 0.0 6 198 1 153 178 0.0 6 176 2 154 241 0.0 6 240 1 155 158 0.0 6 158 156 161 0.0 6 158 2 0 0 1 157 183 0.0 6 183 158 137 0.0 6 137 159 132 0.0 6 130 2 160 169 0.0 6 167 2 161 174 0.0 6 173 1 162 193 0.0 6 193 163 179 0.0 6 179 164 175 0.0 6 174 1 165 177 0.0 6 175 2 166 206 0.0 6 206 167 145 0.0 6 144 1 168 173 0.0 6 172 1 169 201 0.0 6 199 2 170 229 0.0 6 225 4 171 190 0.0 6 185 4 1 172 212 0.0 6 208 3 1 173 207 0.0 6 204 3 174 226 0.0 6 224 2 175 213 0.0 6 210 2 0 0 1 176 217 0.0 6 214 2 0 1 177 213 0.0 6 210 1 1 1 178 245 0.0 6 244 1 179 199 0.0 6 199 180 264 0.0 6 261 2 1 181 688 0.0 6 685 3 182 330 0.0 6 328 2 183 229 0.0 6 228 1 184 247 0.0 6 246 1 185 292 0.0 6 291 1 186 337 0.0 6 332 5 187 309 0.0 6 305 3 1 188 384 0.0 6 376 6 2 189 279 0.0 6 274 5 190 291 0.0 6 286 5 191 318 0.0 6 313 5 192 321 0.0 6 316 4 0 1 193 354 0.0 6 351 3 194 389 0.0 6 385 3 1 195 403 0.0 6 396 6 1 196 505 0.0 6 499 5 1 197 470 0.0 6 462 5 0 0 2 0 1 198 590 0.0 6 575 15 199 665 0.0 6 658 5 1 0 0 0 1 200 764 0.0 6 751 12 1 201 587 0.0 6 579 8 202 584 0.0 6 577 5 1 0 1 203 606 0.0 6 597 8 0 0 0 1 204 573 0.0 6 566 7 205 747 0.0 6 739 8 206 610 0.0 6 603 6 1 207 581 0.0 6 574 7 208 685 0.0 6 671 13 1 209 582 0.0 6 574 7 0 1 210 641 0.0 6 637 4 211 509 0.0 6 507 2 212 540 0.0 6 533 6 1 213 706 0.0 6 694 11 0 0 1 214 736 0.0 6 728 8 215 639 0.0 6 632 6 1 216 784 0.0 6 772 11 1 217 871 0.0 6 859 12 218 667 0.0 6 658 7 0 1 1 219 660 0.0 6 655 5 220 701 0.0 6 689 10 0 1 1 221 690 0.0 6 678 12 222 785 0.0 6 769 14 1 1 223 831 0.0 6 818 12 0 0 0 0 1 224 749 0.0 6 733 13 1 0 0 2 225 655 0.0 6 648 7 226 667 0.0 6 661 6 227 735 0.0 6 728 6 0 1 228 609 0.0 6 604 3 0 0 0 1 1 229 558 0.0 6 556 1 0 0 1 230 503 0.0 6 497 6 231 478 0.0 6 471 7 232 543 0.0 6 535 8 233 581 0.0 6 574 7 234 624 0.0 6 620 4 235 692 0.0 6 682 10 236 807 0.0 6 795 12 237 805 0.0 6 792 13 238 904 0.0 6 886 17 1 239 770 0.0 6 759 11 240 788 0.0 6 772 16 241 919 0.0 6 903 15 1 242 712 0.0 6 693 17 2 243 769 0.0 6 754 11 3 1 244 667 0.0 6 652 13 2 245 727 0.0 6 714 12 0 1 246 673 0.0 6 658 15 247 804 0.0 6 790 13 0 1 248 635 0.0 6 619 15 0 0 1 249 611 0.0 6 601 10 250 615 0.0 6 599 16 251 611 0.0 6 603 8 252 528 0.0 6 514 13 1 253 596 0.0 6 586 9 1 254 486 0.0 6 477 9 255 586 0.0 6 573 13 256 619 0.0 6 604 15 257 467 0.0 6 457 10 258 511 0.0 6 495 15 1 259 693 0.0 6 671 20 1 1 260 690 0.0 6 672 17 0 1 261 533 0.0 6 518 11 4 262 386 0.0 6 383 3 263 352 0.0 6 344 7 0 0 1 264 392 0.0 6 388 4 265 474 0.0 6 469 5 266 372 0.0 6 366 6 267 513 0.0 6 491 21 0 1 268 874 0.0 6 849 24 1 269 504 0.0 6 489 15 270 547 0.0 6 539 8 271 573 0.0 6 557 16 272 674 0.0 6 658 16 273 877 0.0 6 862 15 274 797 0.0 6 784 13 275 888 0.0 6 853 33 2 276 973 0.0 6 943 30 277 2037 0.0 6 1966 68 3 278 3715 0.0 6 3598 110 6 0 0 1 279 5920 0.0 6 5784 132 2 2 280 11155 0.0 6 10900 248 6 0 0 0 1 281 3093 0.0 6 3022 68 2 1 282 29 0.0 6 26 1 2 283 3 0.0 6 1 1 1 285 2 0.0 6 2 288 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 913 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 13 366.7 1 13 61 36 0.0 6 36 70 3 0.0 6 3 71 5 0.0 6 5 72 3 0.0 6 3 73 7 0.0 6 7 74 5 0.0 6 5 75 7 0.0 6 7 76 10 0.0 6 10 77 6 0.0 6 6 78 4 0.0 6 4 79 3 0.0 6 3 80 6 0.0 6 6 81 2 0.0 6 2 83 5 0.0 6 5 84 2 0.0 6 2 85 2 0.0 6 2 86 5 0.0 6 5 87 5 0.0 6 5 88 2 0.0 6 2 89 1 0.0 6 1 90 2 0.0 6 2 91 5 0.0 6 5 92 3 0.0 6 3 93 3 0.0 6 3 94 2 0.0 6 2 95 4 0.0 6 4 96 5 0.0 6 5 97 3 0.0 6 3 98 6 0.0 6 6 99 2 0.0 6 2 100 2 0.0 6 2 101 3 0.0 6 3 102 3 0.0 6 3 103 2 0.0 6 2 104 4 0.0 6 4 105 3 0.0 6 3 106 3 0.0 6 3 107 6 0.0 6 6 108 14 0.0 6 14 109 5 0.0 6 5 110 3 0.0 6 3 111 1 0.0 6 1 112 2 0.0 6 2 113 6 0.0 6 6 114 8 0.0 6 8 115 4 0.0 6 4 116 2 0.0 6 2 117 7 0.0 6 7 118 3 0.0 6 3 119 3 0.0 6 3 120 4 0.0 6 4 121 2 0.0 6 2 122 5 0.0 6 5 123 5 0.0 6 5 124 4 0.0 6 4 125 5 0.0 6 5 126 4 0.0 6 4 127 6 0.0 6 6 128 5 0.0 6 5 129 3 0.0 6 3 130 7 0.0 6 7 131 3 0.0 6 3 132 8 0.0 6 8 133 2 0.0 6 2 134 2 0.0 6 2 135 4 0.0 6 4 136 4 0.0 6 4 137 5 0.0 6 5 138 6 0.0 6 6 139 5 0.0 6 5 140 6 0.0 6 6 141 4 0.0 6 4 142 5 0.0 6 5 143 6 0.0 6 6 144 5 0.0 6 5 145 2 0.0 6 2 146 5 0.0 6 5 147 3 0.0 6 3 148 10 0.0 6 10 149 11 0.0 6 11 150 5 0.0 6 5 151 3 0.0 6 3 152 6 0.0 6 6 153 6 0.0 6 6 154 4 0.0 6 4 155 3 0.0 6 3 156 6 0.0 6 6 157 1 0.0 6 1 158 5 0.0 6 5 159 3 0.0 6 3 160 12 0.0 6 12 161 10 0.0 6 10 162 8 0.0 6 8 163 3 0.0 6 3 164 13 0.0 6 13 165 10 0.0 6 10 166 3 0.0 6 3 167 8 0.0 6 8 168 9 0.0 6 9 169 5 0.0 6 5 170 4 0.0 6 4 171 6 0.0 6 6 172 6 0.0 6 6 173 7 0.0 6 7 174 2 0.0 6 2 175 4 0.0 6 4 176 17 0.0 6 17 177 6 0.0 6 6 178 3 0.0 6 3 179 8 0.0 6 8 180 7 0.0 6 7 181 5 0.0 6 5 182 6 0.0 6 6 183 8 0.0 6 8 184 5 0.0 6 5 185 3 0.0 6 3 186 5 0.0 6 5 187 8 0.0 6 8 188 8 0.0 6 8 189 10 0.0 6 10 190 12 0.0 6 12 191 3 0.0 6 3 192 5 0.0 6 5 193 3 0.0 6 3 194 3 0.0 6 3 195 3 0.0 6 3 196 8 0.0 6 8 197 4 0.0 6 4 198 7 0.0 6 7 199 6 0.0 6 6 200 4 0.0 6 4 201 7 0.0 6 7 202 3 0.0 6 3 203 8 0.0 6 8 204 2 0.0 6 2 205 2 0.0 6 2 206 6 0.0 6 6 207 4 0.0 6 4 208 2 0.0 6 2 209 9 0.0 6 9 210 9 0.0 6 9 211 4 0.0 6 4 212 6 0.0 6 6 213 5 0.0 6 5 214 2 0.0 6 2 215 1 0.0 6 1 216 9 0.0 6 9 217 2 0.0 6 2 218 6 0.0 6 6 219 2 0.0 6 2 220 2 0.0 6 2 221 1 0.0 6 1 222 9 0.0 6 9 223 1 0.0 6 1 224 2 0.0 6 2 225 7 0.0 6 7 226 1 0.0 6 1 227 5 0.0 6 5 228 5 0.0 6 5 229 2 0.0 6 2 230 1 0.0 6 1 231 2 0.0 6 2 232 3 0.0 6 3 233 1 0.0 6 1 234 2 0.0 6 2 235 3 0.0 6 3 236 2 0.0 6 2 237 4 0.0 6 4 238 2 0.0 6 2 239 4 0.0 6 4 240 4 0.0 6 4 241 3 0.0 6 3 242 1 0.0 6 1 243 2 0.0 6 1 1 244 3 0.0 6 3 245 3 0.0 6 3 246 3 0.0 6 3 249 2 0.0 6 2 250 3 0.0 6 3 251 2 0.0 6 2 252 2 0.0 6 2 253 1 0.0 6 1 254 2 0.0 6 2 257 1 0.0 6 1 258 1 0.0 6 1 259 1 0.0 6 1 261 1 0.0 6 1 263 1 0.0 6 1 267 1 0.0 6 1 268 1 0.0 6 1 269 4 0.0 6 4 270 1 0.0 6 1 271 1 0.0 6 1 272 2 0.0 6 2 273 1 0.0 6 1 274 1 0.0 6 1 276 1 0.0 6 1 277 1 0.0 6 1 281 2 0.0 6 2 286 1 0.0 6 1 287 1 0.0 6 1 289 1 0.0 6 1 300 1 0.0 6 1 301 1 0.0 6 1 303 1 0.0 6 1 305 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 93139 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 366.7 1 1 5 1 91.7 1 1 6 4 22.9 1 3 1 7 1 5.7 2 1 10 5 0.1 3 5 11 7 0.0 3 7 12 2 0.0 3 1 0 0 1 13 21 0.0 3 16 5 14 10 0.0 4 7 1 1 1 15 2 0.0 4 1 1 16 24 0.0 4 20 3 1 17 40 0.0 5 35 5 18 39 0.0 5 36 2 1 19 19 0.0 5 15 2 1 1 20 148 0.0 6 142 6 21 87 0.0 6 84 3 22 37 0.0 6 34 3 23 54 0.0 6 51 3 24 46 0.0 6 44 2 25 33 0.0 6 26 5 2 26 16 0.0 6 13 3 27 24 0.0 6 23 1 28 20 0.0 6 19 1 29 27 0.0 6 22 4 1 30 25 0.0 6 25 31 23 0.0 6 22 1 32 19 0.0 6 18 1 33 14 0.0 6 13 1 34 20 0.0 6 20 35 11 0.0 6 11 36 14 0.0 6 12 0 2 37 17 0.0 6 15 1 0 0 1 38 17 0.0 6 16 0 1 39 15 0.0 6 13 2 40 17 0.0 6 14 2 0 1 41 18 0.0 6 15 3 42 15 0.0 6 14 1 43 19 0.0 6 18 1 44 24 0.0 6 23 1 45 13 0.0 6 13 46 29 0.0 6 28 1 47 21 0.0 6 19 2 48 21 0.0 6 19 2 49 29 0.0 6 29 50 31 0.0 6 27 3 1 51 17 0.0 6 17 52 29 0.0 6 26 3 53 31 0.0 6 28 2 1 54 30 0.0 6 28 2 55 21 0.0 6 17 2 0 0 2 56 29 0.0 6 27 2 57 35 0.0 6 30 4 0 0 1 58 29 0.0 6 27 2 59 15 0.0 6 14 1 60 44 0.0 6 37 6 1 61 19 0.0 6 18 1 62 32 0.0 6 27 4 1 63 32 0.0 6 26 4 1 0 0 1 64 29 0.0 6 25 2 2 65 33 0.0 6 31 1 1 66 54 0.0 6 50 4 67 174 0.0 6 164 8 2 68 60 0.0 6 55 3 0 0 2 69 91 0.0 6 90 0 1 70 46 0.0 6 42 4 71 52 0.0 6 50 2 72 40 0.0 6 38 2 73 31 0.0 6 29 2 74 42 0.0 6 38 2 0 0 0 1 1 75 42 0.0 6 38 3 1 76 49 0.0 6 44 2 0 0 1 1 1 77 41 0.0 6 37 3 1 78 37 0.0 6 34 3 79 54 0.0 6 53 1 80 58 0.0 6 50 8 81 44 0.0 6 42 2 82 60 0.0 6 57 3 83 97 0.0 6 91 5 0 0 1 84 62 0.0 6 58 2 2 85 40 0.0 6 39 1 86 40 0.0 6 39 0 1 87 76 0.0 6 68 8 88 50 0.0 6 47 3 89 64 0.0 6 58 5 1 90 68 0.0 6 64 4 91 62 0.0 6 61 1 92 43 0.0 6 41 1 0 0 1 93 93 0.0 6 85 8 94 101 0.0 6 96 4 1 95 92 0.0 6 87 3 0 0 1 1 96 85 0.0 6 81 4 97 83 0.0 6 77 5 0 1 98 86 0.0 6 80 6 99 96 0.0 6 90 5 1 100 109 0.0 6 106 2 1 101 88 0.0 6 85 3 102 120 0.0 6 116 3 0 0 1 103 110 0.0 6 108 2 104 156 0.0 6 153 2 0 0 1 105 115 0.0 6 111 3 0 0 1 106 83 0.0 6 82 0 1 107 91 0.0 6 90 0 0 0 0 0 1 108 100 0.0 6 98 2 109 95 0.0 6 91 4 110 123 0.0 6 116 5 1 0 0 0 1 111 126 0.0 6 118 8 112 145 0.0 6 138 6 1 113 114 0.0 6 107 6 0 0 0 0 1 114 129 0.0 6 125 2 0 1 1 115 142 0.0 6 134 6 1 1 116 143 0.0 6 139 3 0 0 0 1 117 144 0.0 6 139 4 1 118 161 0.0 6 156 2 0 1 0 1 1 119 156 0.0 6 151 4 0 0 1 120 185 0.0 6 176 9 121 147 0.0 6 140 5 0 0 1 0 1 122 146 0.0 6 142 2 2 123 165 0.0 6 159 4 1 0 0 0 1 124 238 0.0 6 229 8 1 125 242 0.0 6 232 8 1 0 0 0 1 126 217 0.0 6 206 7 3 0 0 0 1 127 254 0.0 6 247 6 1 128 220 0.0 6 212 6 1 0 1 129 232 0.0 6 229 3 130 237 0.0 6 233 4 131 331 0.0 6 327 4 132 350 0.0 6 343 6 1 133 420 0.0 6 407 9 0 1 2 1 134 408 0.0 6 392 12 2 1 0 1 135 520 0.0 6 495 22 2 0 1 136 428 0.0 6 420 8 137 442 0.0 6 430 9 2 0 1 138 441 0.0 6 434 5 2 139 559 0.0 6 549 9 0 0 0 1 140 680 0.0 6 667 11 1 0 1 141 665 0.0 6 650 13 0 2 142 764 0.0 6 752 10 1 0 0 1 143 776 0.0 6 761 12 0 2 0 0 1 144 846 0.0 6 832 12 1 0 0 1 145 690 0.0 6 684 5 0 0 1 146 754 0.0 6 741 11 0 0 0 2 147 718 0.0 6 710 5 1 2 148 933 0.0 6 918 12 2 0 0 1 149 1090 0.0 6 1075 15 150 1436 0.0 6 1414 22 151 899 0.0 6 878 18 1 0 1 1 152 1310 0.0 6 1286 21 1 1 0 0 1 153 1088 0.0 6 1059 26 0 0 2 0 1 154 963 0.0 6 947 13 0 1 0 1 1 155 1052 0.0 6 1031 17 2 1 0 0 1 156 893 0.0 6 875 16 2 157 1087 0.0 6 1068 14 0 2 0 3 158 1040 0.0 6 1022 12 2 1 1 1 1 159 802 0.0 6 787 14 1 160 955 0.0 6 934 18 1 1 0 1 161 740 0.0 6 732 7 0 0 0 0 1 162 672 0.0 6 665 6 0 1 163 889 0.0 6 870 17 0 0 0 2 164 844 0.0 6 830 14 165 852 0.0 6 836 16 166 938 0.0 6 918 19 0 0 0 1 167 785 0.0 6 772 13 168 1033 0.0 6 1015 18 169 721 0.0 6 709 10 0 0 0 1 1 170 719 0.0 6 708 10 0 0 0 1 171 1017 0.0 6 1002 14 1 172 892 0.0 6 875 16 0 1 173 994 0.0 6 977 16 0 1 174 1173 0.0 6 1143 26 2 0 2 175 1497 0.0 6 1462 30 1 0 4 176 1168 0.0 6 1140 27 0 0 1 177 972 0.0 6 957 15 178 830 0.0 6 811 17 0 2 179 809 0.0 6 784 20 1 1 2 0 1 180 685 0.0 6 660 23 0 0 2 181 756 0.0 6 738 16 0 0 2 182 480 0.0 6 466 13 0 1 183 1019 0.0 6 994 22 1 0 1 1 184 946 0.0 6 927 16 2 1 185 1087 0.0 6 1056 27 4 186 1367 0.0 6 1345 22 187 1201 0.0 6 1160 39 2 188 1219 0.0 6 1202 15 1 1 189 1077 0.0 6 1039 36 0 0 1 1 190 1536 0.0 6 1488 47 0 1 191 1474 0.0 6 1432 40 0 0 2 192 891 0.0 6 872 19 193 603 0.0 6 586 15 1 1 194 1019 0.0 6 992 24 1 0 0 0 2 195 1652 0.0 6 1606 44 2 196 1736 0.0 6 1688 44 2 0 0 1 1 197 1146 0.0 6 1109 36 1 198 2194 0.0 6 2129 63 1 1 199 1902 0.0 6 1846 53 1 1 1 200 1751 0.0 6 1691 59 1 201 1248 0.0 6 1222 25 1 202 646 0.0 6 631 14 0 0 1 203 797 0.0 6 772 24 1 204 703 0.0 6 691 12 205 369 0.0 6 361 7 1 206 306 0.0 6 300 6 207 365 0.0 6 354 10 0 0 1 208 421 0.0 6 405 16 209 369 0.0 6 354 12 1 1 0 0 1 210 362 0.0 6 348 13 1 211 591 0.0 6 578 11 1 0 1 212 404 0.0 6 392 10 1 1 213 320 0.0 6 310 9 0 1 214 934 0.0 6 899 30 3 1 1 215 583 0.0 6 565 18 216 641 0.0 6 620 20 0 0 0 0 1 217 499 0.0 6 483 14 1 1 218 538 0.0 6 524 12 2 219 438 0.0 6 428 10 220 350 0.0 6 343 6 1 221 574 0.0 6 561 12 0 0 1 222 550 0.0 6 533 17 223 592 0.0 6 577 14 0 0 1 224 486 0.0 6 476 10 225 476 0.0 6 459 17 226 350 0.0 6 338 12 227 316 0.0 6 309 7 228 314 0.0 6 302 10 1 0 1 229 408 0.0 6 392 15 1 230 215 0.0 6 213 2 231 380 0.0 6 366 14 232 327 0.0 6 320 6 1 233 226 0.0 6 218 7 1 234 157 0.0 6 150 7 235 105 0.0 6 102 2 0 0 1 236 60 0.0 6 59 1 237 30 0.0 6 30 238 70 0.0 6 68 2 239 128 0.0 6 116 11 0 1 240 149 0.0 6 145 4 241 343 0.0 6 340 2 1 242 205 0.0 6 202 3 243 108 0.0 6 104 4 244 82 0.0 6 77 4 0 0 0 1 245 85 0.0 6 84 1 246 76 0.0 6 73 3 247 64 0.0 6 63 1 248 95 0.0 6 94 1 249 35 0.0 6 33 2 250 27 0.0 6 25 2 251 29 0.0 6 25 4 252 10 0.0 6 10 253 12 0.0 6 12 254 2 0.0 6 2 256 1 0.0 6 1 265 1 0.0 6 1 266 1 0.0 6 1 277 2 0.0 6 1 1 278 16 0.0 6 16 279 71 0.0 6 69 0 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 669 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 52 1 0.0 6 1 54 1 0.0 6 1 71 1 0.0 6 1 72 4 0.0 6 4 73 9 0.0 6 9 74 14 0.0 6 14 75 4 0.0 6 4 76 4 0.0 6 4 77 5 0.0 6 5 78 3 0.0 6 3 79 8 0.0 6 8 80 8 0.0 6 8 81 3 0.0 6 3 82 7 0.0 6 7 83 7 0.0 6 7 84 4 0.0 6 4 85 5 0.0 6 4 0 0 0 1 86 5 0.0 6 5 87 1 0.0 6 1 88 2 0.0 6 2 89 4 0.0 6 4 90 6 0.0 6 6 91 6 0.0 6 6 92 3 0.0 6 3 93 4 0.0 6 4 94 3 0.0 6 3 95 2 0.0 6 2 96 3 0.0 6 3 97 1 0.0 6 1 98 4 0.0 6 4 99 1 0.0 6 1 100 2 0.0 6 2 101 1 0.0 6 1 102 7 0.0 6 7 103 5 0.0 6 5 104 4 0.0 6 4 105 1 0.0 6 1 106 2 0.0 6 2 107 7 0.0 6 7 108 7 0.0 6 6 0 0 1 109 6 0.0 6 6 110 18 0.0 6 18 111 6 0.0 6 6 112 4 0.0 6 4 113 1 0.0 6 1 114 1 0.0 6 1 115 6 0.0 6 6 116 7 0.0 6 7 117 2 0.0 6 2 118 6 0.0 6 6 119 2 0.0 6 2 120 7 0.0 6 7 121 5 0.0 6 5 122 6 0.0 6 6 123 7 0.0 6 7 124 5 0.0 6 5 125 4 0.0 6 4 126 1 0.0 6 1 127 4 0.0 6 4 128 9 0.0 6 9 129 3 0.0 6 3 130 2 0.0 6 2 131 4 0.0 6 4 132 11 0.0 6 11 133 5 0.0 6 5 134 9 0.0 6 9 135 2 0.0 6 2 136 5 0.0 6 5 137 7 0.0 6 7 138 5 0.0 6 5 139 5 0.0 6 5 140 3 0.0 6 3 141 3 0.0 6 3 142 1 0.0 6 1 143 11 0.0 6 11 144 4 0.0 6 4 145 4 0.0 6 4 146 3 0.0 6 3 147 8 0.0 6 8 148 3 0.0 6 3 149 4 0.0 6 3 0 0 0 1 150 3 0.0 6 3 151 6 0.0 6 6 152 5 0.0 6 5 153 5 0.0 6 5 154 3 0.0 6 3 155 4 0.0 6 4 156 10 0.0 6 10 157 4 0.0 6 4 158 7 0.0 6 7 159 3 0.0 6 3 160 5 0.0 6 5 161 9 0.0 6 9 162 13 0.0 6 13 163 10 0.0 6 10 164 5 0.0 6 5 165 8 0.0 6 8 166 5 0.0 6 5 167 11 0.0 6 11 168 4 0.0 6 4 169 5 0.0 6 5 170 9 0.0 6 9 171 7 0.0 6 7 172 5 0.0 6 5 173 6 0.0 6 5 0 0 1 174 3 0.0 6 3 175 5 0.0 6 5 176 4 0.0 6 4 177 4 0.0 6 4 178 11 0.0 6 11 179 6 0.0 6 6 180 3 0.0 6 3 181 3 0.0 6 3 182 6 0.0 6 6 183 7 0.0 6 7 184 3 0.0 6 3 185 6 0.0 6 6 186 4 0.0 6 4 187 7 0.0 6 7 188 2 0.0 6 2 189 3 0.0 6 3 190 4 0.0 6 4 191 4 0.0 6 4 192 2 0.0 6 2 193 2 0.0 6 2 194 2 0.0 6 2 195 1 0.0 6 1 196 3 0.0 6 2 0 0 0 1 197 5 0.0 6 5 198 5 0.0 6 5 199 1 0.0 6 1 200 4 0.0 6 4 201 2 0.0 6 2 202 3 0.0 6 3 203 3 0.0 6 3 204 1 0.0 6 1 206 3 0.0 6 3 207 3 0.0 6 3 208 1 0.0 6 1 209 2 0.0 6 2 210 1 0.0 6 1 211 1 0.0 6 1 213 1 0.0 6 1 215 1 0.0 6 1 217 1 0.0 6 1 218 1 0.0 6 1 223 1 0.0 6 1 225 1 0.0 6 0 1 235 1 0.0 6 1 245 1 0.0 6 1 246 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_04_87_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_04_314_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_04_87_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_04_314_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.00 s (25 µs/read; 2.36 M reads/minute). === Summary === Total read pairs processed: 78,781 Read 1 with adapter: 78,781 (100.0%) Read 2 with adapter: 78,758 (100.0%) == Read fate breakdown == Pairs that were too short: 36 (0.0%) Pairs discarded as untrimmed: 23 (0.0%) Pairs written (passing filters): 78,722 (99.9%) Total basepairs processed: 33,828,289 bp Read 1: 18,433,196 bp Read 2: 15,395,093 bp Total written (filtered): 4,915,209 bp (14.5%) Read 1: 2,493,262 bp Read 2: 2,421,947 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 76242 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 2 307.7 1 2 6 2 19.2 1 1 0 1 7 1 4.8 2 0 0 1 9 2 0.3 2 1 1 10 1 0.1 3 1 11 2 0.0 3 2 15 2 0.0 4 2 16 1 0.0 4 1 17 2 0.0 5 2 18 1 0.0 5 1 19 1 0.0 5 1 20 156 0.0 6 150 6 21 75 0.0 6 72 3 22 96 0.0 6 93 3 23 62 0.0 6 59 3 24 34 0.0 6 33 1 25 26 0.0 6 24 2 26 25 0.0 6 24 1 27 15 0.0 6 14 1 28 25 0.0 6 24 1 29 19 0.0 6 19 30 8 0.0 6 7 1 31 11 0.0 6 10 1 32 14 0.0 6 11 3 33 3 0.0 6 3 34 11 0.0 6 11 35 7 0.0 6 6 1 36 4 0.0 6 3 0 0 0 0 0 1 37 3 0.0 6 3 38 6 0.0 6 5 1 39 8 0.0 6 8 40 9 0.0 6 9 41 6 0.0 6 6 42 4 0.0 6 4 43 6 0.0 6 5 1 44 11 0.0 6 11 45 5 0.0 6 5 46 6 0.0 6 6 47 4 0.0 6 4 48 5 0.0 6 5 49 4 0.0 6 4 50 4 0.0 6 3 0 0 0 0 1 51 8 0.0 6 7 1 52 9 0.0 6 9 53 9 0.0 6 9 54 5 0.0 6 5 55 4 0.0 6 4 56 3 0.0 6 3 57 8 0.0 6 8 58 8 0.0 6 7 1 59 7 0.0 6 7 60 2 0.0 6 2 61 6 0.0 6 6 62 4 0.0 6 4 63 4 0.0 6 4 64 8 0.0 6 8 65 5 0.0 6 5 66 10 0.0 6 10 67 10 0.0 6 10 68 11 0.0 6 9 1 1 69 4 0.0 6 4 70 8 0.0 6 8 71 8 0.0 6 7 1 72 9 0.0 6 9 73 11 0.0 6 11 74 16 0.0 6 16 75 10 0.0 6 10 76 17 0.0 6 15 2 77 17 0.0 6 16 1 78 6 0.0 6 6 79 8 0.0 6 8 80 5 0.0 6 5 81 12 0.0 6 12 82 15 0.0 6 15 83 10 0.0 6 9 0 0 0 1 84 9 0.0 6 9 85 10 0.0 6 10 86 16 0.0 6 16 87 6 0.0 6 6 88 11 0.0 6 10 1 89 9 0.0 6 8 1 90 10 0.0 6 9 0 0 0 1 91 8 0.0 6 7 1 92 7 0.0 6 6 1 93 12 0.0 6 12 94 13 0.0 6 13 95 14 0.0 6 11 0 1 1 0 0 1 96 10 0.0 6 10 97 10 0.0 6 10 98 10 0.0 6 9 0 0 1 99 13 0.0 6 13 100 11 0.0 6 10 1 101 15 0.0 6 14 1 102 18 0.0 6 17 0 0 0 0 0 1 103 15 0.0 6 15 104 25 0.0 6 23 2 105 17 0.0 6 17 106 22 0.0 6 21 1 107 27 0.0 6 27 108 22 0.0 6 21 0 1 109 22 0.0 6 22 110 21 0.0 6 20 1 111 17 0.0 6 17 112 21 0.0 6 21 113 26 0.0 6 26 114 34 0.0 6 32 2 115 13 0.0 6 13 116 19 0.0 6 17 1 0 1 117 28 0.0 6 26 1 0 1 118 26 0.0 6 25 0 0 0 0 1 119 37 0.0 6 37 120 35 0.0 6 35 121 33 0.0 6 32 1 122 21 0.0 6 21 123 28 0.0 6 28 124 35 0.0 6 34 1 125 25 0.0 6 25 126 34 0.0 6 34 127 29 0.0 6 29 128 40 0.0 6 39 1 129 42 0.0 6 42 130 27 0.0 6 27 131 36 0.0 6 35 1 132 42 0.0 6 41 1 133 38 0.0 6 38 134 33 0.0 6 33 135 44 0.0 6 44 136 40 0.0 6 40 137 47 0.0 6 47 138 49 0.0 6 49 139 55 0.0 6 55 140 312 0.0 6 310 1 0 0 0 0 1 141 279 0.0 6 278 1 142 775 0.0 6 772 3 143 507 0.0 6 506 1 144 177 0.0 6 176 0 0 1 145 231 0.0 6 230 0 0 1 146 391 0.0 6 390 0 1 147 704 0.0 6 704 148 400 0.0 6 398 2 149 231 0.0 6 229 0 1 0 0 0 1 150 455 0.0 6 454 0 0 1 151 569 0.0 6 567 1 0 0 0 0 1 152 529 0.0 6 527 2 153 611 0.0 6 603 6 0 0 0 1 1 154 1338 0.0 6 1311 22 0 0 0 0 5 155 335 0.0 6 331 2 1 0 0 1 156 311 0.0 6 309 2 157 382 0.0 6 381 0 0 1 158 168 0.0 6 168 159 366 0.0 6 366 160 658 0.0 6 656 2 161 734 0.0 6 731 3 162 933 0.0 6 930 3 163 200 0.0 6 199 1 164 149 0.0 6 149 165 328 0.0 6 326 2 166 916 0.0 6 914 2 167 507 0.0 6 505 2 168 625 0.0 6 623 1 0 1 169 251 0.0 6 250 0 1 170 335 0.0 6 334 1 171 235 0.0 6 227 7 1 172 115 0.0 6 111 3 0 0 0 0 1 173 238 0.0 6 237 1 174 173 0.0 6 173 175 131 0.0 6 129 1 1 176 167 0.0 6 164 2 0 0 0 0 1 177 192 0.0 6 192 178 504 0.0 6 502 2 179 540 0.0 6 539 1 180 206 0.0 6 206 181 234 0.0 6 229 4 0 0 0 0 1 182 914 0.0 6 910 4 183 357 0.0 6 356 1 184 272 0.0 6 270 0 0 2 185 658 0.0 6 656 1 0 0 0 0 1 186 1529 0.0 6 1526 3 187 429 0.0 6 427 0 0 2 188 945 0.0 6 942 2 0 1 189 640 0.0 6 638 2 190 584 0.0 6 581 2 0 1 191 603 0.0 6 601 2 192 961 0.0 6 959 2 193 298 0.0 6 296 2 194 401 0.0 6 396 4 1 195 1053 0.0 6 1047 6 196 345 0.0 6 341 4 197 321 0.0 6 316 5 198 430 0.0 6 421 7 1 0 0 0 1 199 851 0.0 6 844 4 0 1 1 1 200 602 0.0 6 594 5 0 2 1 201 671 0.0 6 665 5 1 202 822 0.0 6 816 5 0 0 0 0 1 203 581 0.0 6 571 8 0 1 0 1 204 655 0.0 6 651 2 2 205 594 0.0 6 586 6 0 2 206 593 0.0 6 587 4 0 1 0 1 207 1050 0.0 6 1044 5 0 0 0 0 1 208 879 0.0 6 872 7 209 1031 0.0 6 1024 5 0 0 0 1 1 210 1117 0.0 6 1109 6 1 0 0 0 1 211 882 0.0 6 876 5 1 212 729 0.0 6 728 1 213 911 0.0 6 905 6 214 860 0.0 6 852 7 1 215 1338 0.0 6 1333 4 0 1 216 743 0.0 6 740 1 1 1 217 873 0.0 6 864 7 0 2 218 924 0.0 6 921 3 219 696 0.0 6 687 7 1 0 0 1 220 898 0.0 6 893 3 0 2 221 858 0.0 6 854 2 0 2 222 595 0.0 6 590 5 223 676 0.0 6 670 6 224 559 0.0 6 559 225 486 0.0 6 483 3 226 597 0.0 6 593 4 227 827 0.0 6 821 5 1 228 1186 0.0 6 1177 4 4 0 1 229 626 0.0 6 621 4 0 1 230 634 0.0 6 633 0 0 0 0 1 231 647 0.0 6 644 3 232 777 0.0 6 776 1 233 695 0.0 6 692 1 1 1 234 975 0.0 6 967 8 235 1406 0.0 6 1399 5 1 1 236 589 0.0 6 585 3 1 237 471 0.0 6 469 1 1 238 582 0.0 6 580 1 0 1 239 361 0.0 6 359 1 1 240 403 0.0 6 399 1 1 2 241 429 0.0 6 429 242 517 0.0 6 513 2 0 0 0 1 1 243 721 0.0 6 716 4 1 244 758 0.0 6 753 5 245 398 0.0 6 394 4 246 408 0.0 6 404 3 0 0 0 0 1 247 489 0.0 6 486 2 1 248 433 0.0 6 427 3 2 1 249 222 0.0 6 221 1 250 274 0.0 6 272 2 251 324 0.0 6 322 2 252 289 0.0 6 286 2 1 253 332 0.0 6 330 0 1 0 0 1 254 286 0.0 6 282 3 1 255 355 0.0 6 353 2 256 368 0.0 6 364 1 2 1 257 481 0.0 6 477 3 1 258 181 0.0 6 179 1 0 0 0 0 1 259 199 0.0 6 199 260 179 0.0 6 175 3 1 261 167 0.0 6 165 1 1 262 189 0.0 6 188 1 263 160 0.0 6 156 2 1 1 264 157 0.0 6 157 265 178 0.0 6 177 1 266 217 0.0 6 216 1 267 229 0.0 6 227 1 0 1 268 266 0.0 6 265 1 269 349 0.0 6 345 3 0 1 270 201 0.0 6 196 4 0 1 271 203 0.0 6 199 2 2 272 204 0.0 6 200 1 2 1 273 211 0.0 6 207 3 0 1 274 190 0.0 6 189 0 0 1 275 238 0.0 6 238 276 181 0.0 6 181 277 182 0.0 6 181 1 278 263 0.0 6 260 3 279 282 0.0 6 280 2 280 1220 0.0 6 1208 8 2 1 1 281 569 0.0 6 559 8 1 0 0 0 1 282 5 0.0 6 5 283 3 0.0 6 3 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2539 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 5 307.7 1 5 60 1 0.0 6 0 1 61 4 0.0 6 4 69 5 0.0 6 5 70 6 0.0 6 6 71 15 0.0 6 15 72 28 0.0 6 28 73 18 0.0 6 18 74 6 0.0 6 6 75 13 0.0 6 12 1 76 27 0.0 6 27 77 38 0.0 6 38 78 14 0.0 6 14 79 12 0.0 6 12 80 20 0.0 6 20 81 16 0.0 6 16 82 18 0.0 6 18 83 23 0.0 6 23 84 27 0.0 6 27 85 5 0.0 6 5 86 10 0.0 6 10 87 13 0.0 6 13 88 6 0.0 6 6 89 19 0.0 6 19 90 37 0.0 6 37 91 39 0.0 6 39 92 37 0.0 6 37 93 2 0.0 6 2 94 2 0.0 6 2 95 13 0.0 6 13 96 25 0.0 6 25 97 14 0.0 6 14 98 17 0.0 6 17 99 5 0.0 6 5 100 8 0.0 6 8 101 5 0.0 6 5 102 1 0.0 6 1 103 3 0.0 6 3 105 1 0.0 6 1 107 4 0.0 6 4 108 19 0.0 6 19 109 18 0.0 6 18 110 1 0.0 6 1 111 14 0.0 6 14 112 52 0.0 6 52 113 5 0.0 6 5 114 9 0.0 6 8 1 115 39 0.0 6 39 116 58 0.0 6 57 1 117 12 0.0 6 12 118 32 0.0 6 32 119 20 0.0 6 20 120 20 0.0 6 20 121 24 0.0 6 24 122 30 0.0 6 29 1 123 5 0.0 6 5 124 7 0.0 6 7 125 33 0.0 6 33 126 4 0.0 6 4 128 5 0.0 6 5 129 15 0.0 6 15 130 2 0.0 6 2 131 4 0.0 6 4 132 14 0.0 6 14 133 7 0.0 6 7 134 23 0.0 6 23 135 4 0.0 6 4 136 15 0.0 6 15 137 30 0.0 6 30 138 17 0.0 6 17 139 27 0.0 6 27 140 15 0.0 6 15 141 13 0.0 6 13 142 33 0.0 6 33 143 16 0.0 6 16 144 7 0.0 6 7 145 31 0.0 6 31 146 10 0.0 6 10 147 19 0.0 6 19 148 37 0.0 6 37 149 11 0.0 6 11 150 41 0.0 6 41 151 29 0.0 6 29 152 8 0.0 6 8 153 1 0.0 6 1 154 1 0.0 6 0 0 0 0 0 0 1 156 3 0.0 6 2 0 0 0 0 0 1 157 19 0.0 6 19 158 78 0.0 6 78 159 4 0.0 6 4 160 6 0.0 6 6 161 2 0.0 6 2 162 3 0.0 6 3 163 14 0.0 6 14 164 40 0.0 6 40 165 77 0.0 6 77 166 36 0.0 6 35 1 167 2 0.0 6 2 170 1 0.0 6 1 171 6 0.0 6 6 172 20 0.0 6 20 173 91 0.0 6 91 174 87 0.0 6 86 1 175 12 0.0 6 12 176 20 0.0 6 20 177 37 0.0 6 37 178 53 0.0 6 53 179 2 0.0 6 2 183 4 0.0 6 4 184 7 0.0 6 7 185 6 0.0 6 6 186 28 0.0 6 28 187 14 0.0 6 14 189 1 0.0 6 1 194 2 0.0 6 2 195 4 0.0 6 4 196 1 0.0 6 1 197 11 0.0 6 11 198 55 0.0 6 55 199 5 0.0 6 5 200 8 0.0 6 8 201 16 0.0 6 16 202 1 0.0 6 1 203 4 0.0 6 4 204 9 0.0 6 9 205 5 0.0 6 5 211 1 0.0 6 1 212 2 0.0 6 2 214 1 0.0 6 1 217 3 0.0 6 3 218 7 0.0 6 7 219 21 0.0 6 21 220 57 0.0 6 57 221 1 0.0 6 1 222 2 0.0 6 2 223 1 0.0 6 1 224 2 0.0 6 2 226 2 0.0 6 2 227 4 0.0 6 4 228 1 0.0 6 1 230 3 0.0 6 3 231 10 0.0 6 10 232 12 0.0 6 12 233 1 0.0 6 1 234 2 0.0 6 2 235 2 0.0 6 2 238 1 0.0 6 1 239 3 0.0 6 3 242 2 0.0 6 2 243 32 0.0 6 32 244 56 0.0 6 56 245 19 0.0 6 19 248 1 0.0 6 1 249 2 0.0 6 2 250 8 0.0 6 8 251 3 0.0 6 3 252 1 0.0 6 1 258 1 0.0 6 1 259 8 0.0 6 8 260 42 0.0 6 42 281 4 0.0 6 4 282 3 0.0 6 3 287 1 0.0 6 1 289 1 0.0 6 1 292 2 0.0 6 2 293 4 0.0 6 4 294 13 0.0 6 13 299 2 0.0 6 2 303 1 0.0 6 1 310 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 75519 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 76.9 1 1 7 2 4.8 2 2 10 1 0.1 3 1 11 5 0.0 3 2 3 12 2 0.0 3 2 13 4 0.0 3 4 14 11 0.0 4 10 0 0 1 15 4 0.0 4 2 1 1 16 14 0.0 4 14 17 11 0.0 5 11 18 4 0.0 5 2 1 0 1 19 42 0.0 5 35 7 20 91 0.0 6 85 6 21 60 0.0 6 56 4 22 46 0.0 6 42 4 23 45 0.0 6 43 2 24 33 0.0 6 29 4 25 21 0.0 6 19 1 1 26 24 0.0 6 23 1 27 20 0.0 6 19 1 28 18 0.0 6 18 29 15 0.0 6 13 2 30 16 0.0 6 16 31 13 0.0 6 11 1 0 0 1 32 16 0.0 6 15 0 0 0 0 0 1 33 9 0.0 6 9 34 19 0.0 6 14 4 0 0 1 35 10 0.0 6 10 36 13 0.0 6 12 1 37 10 0.0 6 8 1 1 38 10 0.0 6 9 1 39 11 0.0 6 10 1 40 7 0.0 6 7 41 9 0.0 6 8 1 42 10 0.0 6 10 43 18 0.0 6 17 1 44 15 0.0 6 11 1 1 0 0 0 2 45 8 0.0 6 7 0 0 0 0 0 1 46 15 0.0 6 12 2 0 1 47 14 0.0 6 13 0 0 0 0 0 1 48 21 0.0 6 15 6 49 21 0.0 6 18 1 1 0 1 50 13 0.0 6 12 1 51 17 0.0 6 15 1 0 0 0 0 1 52 16 0.0 6 13 2 1 53 29 0.0 6 27 2 54 24 0.0 6 23 1 55 18 0.0 6 16 1 0 0 1 56 23 0.0 6 17 5 1 57 25 0.0 6 25 58 17 0.0 6 17 59 20 0.0 6 18 1 1 60 20 0.0 6 19 0 0 0 0 0 1 61 22 0.0 6 19 2 1 62 15 0.0 6 14 0 1 63 16 0.0 6 15 1 64 15 0.0 6 13 1 0 0 0 0 1 65 29 0.0 6 25 3 0 0 1 66 30 0.0 6 29 1 67 21 0.0 6 21 68 21 0.0 6 20 1 69 41 0.0 6 40 1 70 148 0.0 6 144 4 71 40 0.0 6 40 72 62 0.0 6 57 3 0 0 2 73 42 0.0 6 39 3 74 49 0.0 6 46 3 75 27 0.0 6 26 1 76 19 0.0 6 15 2 1 0 1 77 37 0.0 6 37 78 36 0.0 6 31 4 0 1 79 28 0.0 6 26 2 80 32 0.0 6 30 2 81 26 0.0 6 24 2 82 36 0.0 6 35 1 83 56 0.0 6 51 2 0 0 1 1 1 84 55 0.0 6 52 3 85 52 0.0 6 44 4 1 0 2 1 86 72 0.0 6 70 1 0 0 0 0 1 87 26 0.0 6 24 2 88 37 0.0 6 34 2 1 89 39 0.0 6 39 90 42 0.0 6 41 1 91 33 0.0 6 33 92 52 0.0 6 49 2 1 93 34 0.0 6 32 1 0 0 1 94 55 0.0 6 52 2 1 95 41 0.0 6 39 2 96 57 0.0 6 57 97 54 0.0 6 52 2 98 70 0.0 6 68 2 99 62 0.0 6 59 2 0 0 1 100 36 0.0 6 34 2 101 67 0.0 6 62 5 102 47 0.0 6 46 1 103 43 0.0 6 40 1 1 1 104 56 0.0 6 55 1 105 57 0.0 6 55 2 106 77 0.0 6 75 2 107 144 0.0 6 137 6 0 0 0 1 108 88 0.0 6 84 2 0 1 1 109 87 0.0 6 84 2 0 0 0 1 110 82 0.0 6 80 2 111 83 0.0 6 80 3 112 85 0.0 6 82 3 113 110 0.0 6 107 0 2 0 1 114 113 0.0 6 110 3 115 106 0.0 6 102 4 116 119 0.0 6 116 2 1 117 125 0.0 6 122 1 1 0 1 118 140 0.0 6 136 4 119 110 0.0 6 108 2 120 111 0.0 6 109 2 121 153 0.0 6 146 6 1 122 146 0.0 6 144 2 123 110 0.0 6 110 124 117 0.0 6 114 2 0 0 0 1 125 117 0.0 6 114 3 126 125 0.0 6 122 3 127 158 0.0 6 151 6 0 0 0 1 128 178 0.0 6 177 1 129 175 0.0 6 173 2 130 188 0.0 6 184 3 0 1 131 203 0.0 6 201 2 132 217 0.0 6 213 4 133 317 0.0 6 310 6 0 0 1 134 253 0.0 6 246 5 1 0 0 1 135 369 0.0 6 365 2 0 0 2 136 416 0.0 6 407 8 0 0 0 1 137 419 0.0 6 414 5 138 652 0.0 6 642 9 0 0 1 139 550 0.0 6 545 5 140 837 0.0 6 831 6 141 547 0.0 6 543 4 142 527 0.0 6 520 4 2 0 0 1 143 656 0.0 6 646 10 144 865 0.0 6 846 18 0 0 1 145 1115 0.0 6 1103 11 0 0 0 1 146 795 0.0 6 784 9 0 0 2 147 754 0.0 6 742 12 148 955 0.0 6 949 6 149 945 0.0 6 935 7 1 0 0 1 1 150 908 0.0 6 893 14 1 151 1132 0.0 6 1118 12 2 152 1110 0.0 6 1101 6 1 0 2 153 1193 0.0 6 1185 7 1 154 1062 0.0 6 1051 10 0 1 155 1189 0.0 6 1171 18 156 1272 0.0 6 1252 16 1 1 2 157 1111 0.0 6 1097 8 1 0 5 158 1440 0.0 6 1424 13 3 159 1163 0.0 6 1149 11 2 0 1 160 1350 0.0 6 1334 12 0 1 2 1 161 931 0.0 6 918 8 3 0 1 1 162 984 0.0 6 973 10 0 0 1 163 1099 0.0 6 1085 10 1 1 2 164 1202 0.0 6 1192 7 2 1 165 881 0.0 6 871 9 0 1 166 1001 0.0 6 990 8 2 0 0 1 167 989 0.0 6 979 9 0 0 1 168 875 0.0 6 865 10 169 976 0.0 6 958 16 0 1 0 1 170 836 0.0 6 825 10 1 171 947 0.0 6 932 14 0 0 0 0 1 172 893 0.0 6 883 9 1 173 822 0.0 6 816 5 0 0 0 1 174 951 0.0 6 945 6 175 1058 0.0 6 1053 4 0 0 1 176 1011 0.0 6 1003 7 1 177 1064 0.0 6 1052 11 0 0 0 0 1 178 1023 0.0 6 1017 5 0 0 0 1 179 1308 0.0 6 1289 16 0 0 0 3 180 1069 0.0 6 1062 7 181 1083 0.0 6 1077 5 0 0 0 0 1 182 890 0.0 6 877 12 0 0 0 1 183 893 0.0 6 883 9 0 0 1 184 953 0.0 6 946 4 0 0 0 2 1 185 1062 0.0 6 1049 11 1 0 0 1 186 1264 0.0 6 1253 11 187 1450 0.0 6 1436 13 0 0 0 1 188 1432 0.0 6 1415 16 0 0 0 1 189 789 0.0 6 780 7 0 0 1 1 190 967 0.0 6 959 8 191 843 0.0 6 838 5 192 748 0.0 6 743 5 193 657 0.0 6 654 3 194 648 0.0 6 645 1 1 0 1 195 436 0.0 6 432 3 0 0 1 196 523 0.0 6 519 4 197 515 0.0 6 512 3 198 515 0.0 6 510 4 0 0 1 199 657 0.0 6 653 3 1 200 560 0.0 6 548 9 2 0 0 1 201 510 0.0 6 505 5 202 480 0.0 6 477 3 203 414 0.0 6 409 5 204 420 0.0 6 415 5 205 330 0.0 6 326 4 206 295 0.0 6 290 5 207 366 0.0 6 359 7 208 345 0.0 6 343 1 0 0 0 0 1 209 541 0.0 6 535 5 0 1 210 390 0.0 6 387 3 211 373 0.0 6 365 7 0 0 1 212 307 0.0 6 306 1 213 204 0.0 6 202 2 214 207 0.0 6 206 1 215 337 0.0 6 337 216 299 0.0 6 296 2 0 0 1 217 283 0.0 6 280 3 218 194 0.0 6 192 2 219 219 0.0 6 215 2 0 0 2 220 141 0.0 6 140 1 221 176 0.0 6 175 0 0 0 1 222 107 0.0 6 105 2 223 94 0.0 6 94 224 123 0.0 6 122 1 225 117 0.0 6 114 3 226 149 0.0 6 149 227 60 0.0 6 58 2 228 72 0.0 6 71 1 229 89 0.0 6 88 1 230 78 0.0 6 78 231 79 0.0 6 78 1 232 82 0.0 6 82 233 65 0.0 6 63 1 1 234 67 0.0 6 66 1 235 41 0.0 6 40 1 236 19 0.0 6 17 2 237 35 0.0 6 34 0 0 0 1 238 29 0.0 6 29 239 16 0.0 6 16 240 27 0.0 6 26 0 0 0 1 241 38 0.0 6 38 242 21 0.0 6 21 243 8 0.0 6 8 244 23 0.0 6 22 1 245 14 0.0 6 14 246 13 0.0 6 13 247 6 0.0 6 5 1 248 12 0.0 6 12 249 6 0.0 6 6 250 6 0.0 6 6 251 11 0.0 6 10 1 252 5 0.0 6 5 253 3 0.0 6 3 254 8 0.0 6 8 256 4 0.0 6 4 257 1 0.0 6 1 258 1 0.0 6 1 259 1 0.0 6 1 260 1 0.0 6 1 280 1 0.0 6 1 281 3 0.0 6 3 282 83 0.0 6 83 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 3239 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 17 1 0.0 5 0 0 0 0 1 46 1 0.0 6 0 1 52 2 0.0 6 2 68 2 0.0 6 2 69 8 0.0 6 8 70 10 0.0 6 9 0 0 0 0 0 1 71 30 0.0 6 30 72 29 0.0 6 29 73 6 0.0 6 6 74 10 0.0 6 10 75 16 0.0 6 16 76 38 0.0 6 38 77 11 0.0 6 11 78 4 0.0 6 4 79 10 0.0 6 10 80 27 0.0 6 26 0 0 1 81 16 0.0 6 16 82 36 0.0 6 31 5 83 1046 0.0 6 1018 28 84 15 0.0 6 15 85 15 0.0 6 15 86 19 0.0 6 17 0 0 1 1 87 9 0.0 6 5 0 0 0 4 88 18 0.0 6 18 89 33 0.0 6 33 90 33 0.0 6 33 91 39 0.0 6 39 92 1 0.0 6 1 93 2 0.0 6 2 94 18 0.0 6 17 0 0 0 1 95 30 0.0 6 30 96 15 0.0 6 15 97 33 0.0 6 33 98 10 0.0 6 10 99 14 0.0 6 14 100 5 0.0 6 5 101 2 0.0 6 1 0 0 0 1 102 7 0.0 6 7 103 4 0.0 6 4 105 2 0.0 6 1 1 107 21 0.0 6 21 108 13 0.0 6 13 110 5 0.0 6 5 111 42 0.0 6 42 112 3 0.0 6 3 113 5 0.0 6 5 114 22 0.0 6 22 115 66 0.0 6 65 0 0 0 1 116 6 0.0 6 5 1 117 39 0.0 6 39 118 22 0.0 6 22 119 19 0.0 6 19 120 21 0.0 6 21 121 41 0.0 6 41 122 8 0.0 6 7 0 0 0 1 123 10 0.0 6 10 124 40 0.0 6 39 0 0 1 125 1 0.0 6 1 127 3 0.0 6 3 128 21 0.0 6 21 129 1 0.0 6 1 130 2 0.0 6 2 131 21 0.0 6 21 132 1 0.0 6 1 133 21 0.0 6 21 134 5 0.0 6 5 135 18 0.0 6 18 136 33 0.0 6 32 0 0 1 137 5 0.0 6 5 138 36 0.0 6 35 1 139 14 0.0 6 14 140 14 0.0 6 14 141 36 0.0 6 36 142 20 0.0 6 20 143 10 0.0 6 10 144 34 0.0 6 34 145 13 0.0 6 12 1 146 12 0.0 6 11 0 0 0 1 147 27 0.0 6 27 148 6 0.0 6 5 1 149 29 0.0 6 29 150 37 0.0 6 37 151 6 0.0 6 6 154 1 0.0 6 1 155 1 0.0 6 1 156 6 0.0 6 6 157 82 0.0 6 82 158 2 0.0 6 2 159 6 0.0 6 5 0 0 0 1 160 1 0.0 6 1 161 7 0.0 6 6 0 0 0 1 162 6 0.0 6 6 163 59 0.0 6 59 164 76 0.0 6 76 165 41 0.0 6 41 166 2 0.0 6 2 169 1 0.0 6 1 170 5 0.0 6 5 171 16 0.0 6 16 172 65 0.0 6 65 173 89 0.0 6 89 174 4 0.0 6 4 175 10 0.0 6 8 0 0 1 1 176 31 0.0 6 31 177 63 0.0 6 62 0 0 0 1 178 1 0.0 6 1 181 2 0.0 6 2 182 1 0.0 6 1 183 6 0.0 6 6 184 2 0.0 6 2 185 25 0.0 6 25 186 19 0.0 6 19 187 2 0.0 6 2 193 1 0.0 6 1 194 2 0.0 6 2 195 2 0.0 6 2 196 10 0.0 6 10 197 52 0.0 6 52 198 1 0.0 6 1 199 4 0.0 6 4 200 23 0.0 6 23 203 5 0.0 6 5 204 1 0.0 6 1 211 1 0.0 6 1 216 3 0.0 6 3 217 3 0.0 6 3 218 5 0.0 6 5 219 21 0.0 6 21 220 1 0.0 6 1 221 1 0.0 6 1 226 2 0.0 6 2 230 4 0.0 6 4 231 18 0.0 6 18 234 2 0.0 6 2 237 1 0.0 6 1 242 1 0.0 6 1 243 6 0.0 6 6 244 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_05_88_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_05_315_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_05_88_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_05_315_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.79 s (24 µs/read; 2.55 M reads/minute). === Summary === Total read pairs processed: 75,819 Read 1 with adapter: 75,819 (100.0%) Read 2 with adapter: 75,771 (99.9%) == Read fate breakdown == Pairs that were too short: 26 (0.0%) Pairs discarded as untrimmed: 48 (0.1%) Pairs written (passing filters): 75,745 (99.9%) Total basepairs processed: 33,356,892 bp Read 1: 18,973,393 bp Read 2: 14,383,499 bp Total written (filtered): 4,627,567 bp (13.9%) Read 1: 2,329,381 bp Read 2: 2,298,186 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 74577 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 296.2 1 1 6 3 18.5 1 0 0 3 7 2 4.6 2 2 9 3 0.3 2 2 1 11 1 0.0 3 1 12 1 0.0 3 1 14 3 0.0 4 1 1 0 0 1 15 1 0.0 4 1 16 1 0.0 4 1 17 1 0.0 5 1 18 1 0.0 5 1 19 2 0.0 5 1 1 20 133 0.0 6 130 3 21 77 0.0 6 74 3 22 67 0.0 6 67 23 39 0.0 6 37 2 24 18 0.0 6 17 0 1 25 31 0.0 6 31 26 18 0.0 6 18 27 21 0.0 6 21 28 9 0.0 6 9 29 15 0.0 6 15 30 10 0.0 6 9 1 31 6 0.0 6 6 32 9 0.0 6 9 33 7 0.0 6 6 0 0 0 0 1 34 9 0.0 6 9 35 7 0.0 6 6 1 36 9 0.0 6 9 37 7 0.0 6 7 38 5 0.0 6 5 39 6 0.0 6 4 2 40 5 0.0 6 5 41 4 0.0 6 4 42 5 0.0 6 5 43 7 0.0 6 7 44 13 0.0 6 12 0 0 0 1 45 8 0.0 6 8 46 6 0.0 6 6 47 8 0.0 6 8 48 5 0.0 6 5 49 7 0.0 6 5 2 50 4 0.0 6 4 51 2 0.0 6 2 52 11 0.0 6 10 1 53 10 0.0 6 10 54 9 0.0 6 9 55 9 0.0 6 8 1 56 3 0.0 6 3 57 7 0.0 6 6 1 58 8 0.0 6 8 59 5 0.0 6 5 60 4 0.0 6 4 61 2 0.0 6 2 62 6 0.0 6 6 63 5 0.0 6 4 1 64 8 0.0 6 8 65 5 0.0 6 5 66 6 0.0 6 6 67 8 0.0 6 8 68 6 0.0 6 6 69 11 0.0 6 11 70 6 0.0 6 6 71 5 0.0 6 5 72 8 0.0 6 8 73 13 0.0 6 12 1 74 16 0.0 6 15 1 75 7 0.0 6 7 76 8 0.0 6 7 0 0 0 0 0 1 77 10 0.0 6 9 1 78 15 0.0 6 14 0 0 0 0 0 1 79 7 0.0 6 6 1 80 8 0.0 6 8 81 8 0.0 6 8 82 10 0.0 6 10 83 5 0.0 6 5 84 14 0.0 6 13 1 85 13 0.0 6 13 86 12 0.0 6 12 87 10 0.0 6 10 88 6 0.0 6 6 89 10 0.0 6 10 90 6 0.0 6 6 91 8 0.0 6 8 92 4 0.0 6 3 1 93 7 0.0 6 6 0 0 0 1 94 10 0.0 6 9 1 95 6 0.0 6 6 96 12 0.0 6 12 97 5 0.0 6 5 98 7 0.0 6 7 99 9 0.0 6 9 100 8 0.0 6 7 1 101 10 0.0 6 10 102 15 0.0 6 14 1 103 17 0.0 6 17 104 16 0.0 6 16 105 19 0.0 6 19 106 31 0.0 6 31 107 23 0.0 6 23 108 18 0.0 6 17 0 1 109 23 0.0 6 22 1 110 10 0.0 6 9 0 1 111 20 0.0 6 20 112 16 0.0 6 16 113 12 0.0 6 12 114 21 0.0 6 21 115 21 0.0 6 20 1 116 19 0.0 6 18 1 117 17 0.0 6 17 118 23 0.0 6 20 0 0 0 0 3 119 25 0.0 6 25 120 19 0.0 6 17 1 0 0 0 0 1 121 23 0.0 6 23 122 22 0.0 6 20 2 123 29 0.0 6 29 124 28 0.0 6 28 125 29 0.0 6 28 1 126 21 0.0 6 21 127 31 0.0 6 31 128 30 0.0 6 29 1 129 18 0.0 6 18 130 39 0.0 6 39 131 39 0.0 6 39 132 39 0.0 6 39 133 25 0.0 6 25 134 23 0.0 6 23 135 32 0.0 6 32 136 38 0.0 6 38 137 42 0.0 6 42 138 39 0.0 6 39 139 53 0.0 6 51 2 140 73 0.0 6 72 1 141 141 0.0 6 138 3 142 299 0.0 6 299 143 435 0.0 6 434 1 144 281 0.0 6 281 145 271 0.0 6 270 1 146 391 0.0 6 390 1 147 271 0.0 6 270 0 0 1 148 322 0.0 6 321 0 0 1 149 352 0.0 6 351 1 150 331 0.0 6 331 151 321 0.0 6 320 1 152 311 0.0 6 309 2 153 227 0.0 6 226 0 1 154 206 0.0 6 205 1 155 233 0.0 6 232 1 156 241 0.0 6 240 1 157 244 0.0 6 243 1 158 271 0.0 6 271 159 255 0.0 6 253 1 0 0 0 0 1 160 286 0.0 6 285 1 161 271 0.0 6 269 2 162 280 0.0 6 280 163 243 0.0 6 242 1 164 331 0.0 6 330 1 165 212 0.0 6 208 3 0 0 0 0 1 166 226 0.0 6 224 2 167 320 0.0 6 316 4 168 240 0.0 6 239 1 169 259 0.0 6 258 1 170 274 0.0 6 269 5 171 278 0.0 6 276 2 172 289 0.0 6 289 173 299 0.0 6 299 174 303 0.0 6 303 175 315 0.0 6 314 1 176 333 0.0 6 331 2 177 275 0.0 6 273 2 178 345 0.0 6 343 2 179 634 0.0 6 630 2 0 0 1 1 180 314 0.0 6 311 3 181 278 0.0 6 276 2 182 291 0.0 6 288 2 0 1 183 284 0.0 6 282 2 184 297 0.0 6 291 5 1 185 339 0.0 6 336 3 186 359 0.0 6 357 2 187 329 0.0 6 328 1 188 358 0.0 6 357 1 189 267 0.0 6 265 2 190 291 0.0 6 287 4 191 347 0.0 6 342 5 192 335 0.0 6 329 4 1 0 0 0 1 193 431 0.0 6 429 2 194 446 0.0 6 444 2 195 417 0.0 6 413 4 196 532 0.0 6 525 5 1 0 1 197 630 0.0 6 627 3 198 651 0.0 6 647 2 1 1 199 677 0.0 6 674 3 200 734 0.0 6 720 12 1 0 1 201 572 0.0 6 564 8 202 529 0.0 6 524 4 0 1 203 746 0.0 6 736 9 0 1 204 524 0.0 6 518 5 0 0 0 1 205 557 0.0 6 555 2 206 594 0.0 6 590 4 207 667 0.0 6 661 6 208 877 0.0 6 868 9 209 687 0.0 6 681 5 1 210 708 0.0 6 698 10 211 548 0.0 6 541 6 0 1 212 621 0.0 6 614 7 213 656 0.0 6 651 4 0 0 0 0 1 214 764 0.0 6 760 4 215 735 0.0 6 727 7 1 216 801 0.0 6 797 4 217 944 0.0 6 932 11 1 218 814 0.0 6 810 4 219 768 0.0 6 763 4 1 220 798 0.0 6 791 6 0 1 221 719 0.0 6 715 4 222 604 0.0 6 596 7 1 223 614 0.0 6 608 5 0 0 0 1 224 656 0.0 6 653 3 225 722 0.0 6 715 5 0 1 0 0 1 226 764 0.0 6 759 5 227 888 0.0 6 876 12 228 843 0.0 6 832 10 0 0 1 229 651 0.0 6 647 4 230 709 0.0 6 704 3 1 0 0 1 231 617 0.0 6 607 9 0 0 1 232 647 0.0 6 643 4 233 698 0.0 6 693 5 234 713 0.0 6 705 8 235 640 0.0 6 633 6 0 0 0 1 236 592 0.0 6 587 3 1 0 0 1 237 546 0.0 6 538 7 1 238 503 0.0 6 497 4 2 239 578 0.0 6 572 6 240 571 0.0 6 564 6 0 0 0 0 1 241 534 0.0 6 529 5 242 571 0.0 6 567 4 243 543 0.0 6 536 7 244 534 0.0 6 529 5 245 533 0.0 6 525 8 246 389 0.0 6 383 5 1 247 400 0.0 6 396 3 1 248 368 0.0 6 366 2 249 378 0.0 6 374 4 250 372 0.0 6 370 2 251 389 0.0 6 385 4 252 392 0.0 6 389 2 1 253 367 0.0 6 362 5 254 384 0.0 6 379 4 1 255 344 0.0 6 340 3 1 256 298 0.0 6 295 3 257 315 0.0 6 312 2 1 258 281 0.0 6 276 4 1 259 381 0.0 6 375 6 260 425 0.0 6 420 4 1 261 344 0.0 6 339 5 262 381 0.0 6 369 11 0 1 263 392 0.0 6 384 6 2 264 300 0.0 6 295 5 265 327 0.0 6 323 4 266 247 0.0 6 246 1 267 272 0.0 6 266 6 268 265 0.0 6 259 4 1 0 0 0 1 269 265 0.0 6 264 0 0 1 270 216 0.0 6 210 5 0 0 0 1 271 359 0.0 6 343 16 272 357 0.0 6 346 10 1 273 247 0.0 6 242 5 274 246 0.0 6 243 3 275 372 0.0 6 360 11 1 276 497 0.0 6 483 13 0 0 0 1 277 503 0.0 6 497 6 278 378 0.0 6 375 2 0 1 279 518 0.0 6 508 10 280 1147 0.0 6 1116 28 2 1 281 9472 0.0 6 9324 143 4 1 282 22 0.0 6 20 2 283 1 0.0 6 1 285 1 0.0 6 1 286 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1242 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 10 296.2 1 10 61 16 0.0 6 16 67 1 0.0 6 1 68 1 0.0 6 1 70 7 0.0 6 7 71 10 0.0 6 10 72 8 0.0 6 8 73 4 0.0 6 4 74 2 0.0 6 2 75 9 0.0 6 9 76 4 0.0 6 4 77 10 0.0 6 10 78 6 0.0 6 6 79 7 0.0 6 7 80 7 0.0 6 7 81 3 0.0 6 3 82 2 0.0 6 2 83 3 0.0 6 3 84 5 0.0 6 5 85 6 0.0 6 6 86 5 0.0 6 5 87 13 0.0 6 13 88 6 0.0 6 6 89 5 0.0 6 5 90 5 0.0 6 5 91 8 0.0 6 8 92 6 0.0 6 6 93 11 0.0 6 11 94 6 0.0 6 6 95 4 0.0 6 4 96 9 0.0 6 9 97 6 0.0 6 6 98 4 0.0 6 4 99 4 0.0 6 4 100 9 0.0 6 9 101 7 0.0 6 7 102 8 0.0 6 8 103 8 0.0 6 8 104 5 0.0 6 5 105 7 0.0 6 7 106 2 0.0 6 2 107 8 0.0 6 8 108 11 0.0 6 11 109 8 0.0 6 8 110 4 0.0 6 4 111 3 0.0 6 3 112 6 0.0 6 6 113 4 0.0 6 4 114 4 0.0 6 4 115 7 0.0 6 7 116 3 0.0 6 3 117 5 0.0 6 5 118 2 0.0 6 2 119 6 0.0 6 6 120 5 0.0 6 5 121 9 0.0 6 9 122 6 0.0 6 6 123 3 0.0 6 3 124 6 0.0 6 6 125 3 0.0 6 3 126 11 0.0 6 11 127 4 0.0 6 4 128 6 0.0 6 6 129 6 0.0 6 6 130 11 0.0 6 11 131 5 0.0 6 5 132 7 0.0 6 7 133 7 0.0 6 7 134 2 0.0 6 2 135 2 0.0 6 2 136 5 0.0 6 5 137 13 0.0 6 13 138 9 0.0 6 9 139 10 0.0 6 10 140 8 0.0 6 8 141 7 0.0 6 7 142 3 0.0 6 3 143 8 0.0 6 8 144 4 0.0 6 4 145 5 0.0 6 5 146 3 0.0 6 3 147 9 0.0 6 9 148 6 0.0 6 6 149 5 0.0 6 5 150 4 0.0 6 4 151 6 0.0 6 6 152 4 0.0 6 4 153 4 0.0 6 4 154 5 0.0 6 5 155 6 0.0 6 6 156 10 0.0 6 10 157 8 0.0 6 8 158 9 0.0 6 9 159 8 0.0 6 8 160 8 0.0 6 8 161 14 0.0 6 14 162 14 0.0 6 14 163 7 0.0 6 7 164 8 0.0 6 8 165 9 0.0 6 9 166 4 0.0 6 4 167 6 0.0 6 6 168 7 0.0 6 7 169 5 0.0 6 5 170 7 0.0 6 7 171 7 0.0 6 7 172 4 0.0 6 4 173 12 0.0 6 12 174 9 0.0 6 9 175 8 0.0 6 8 176 12 0.0 6 12 177 15 0.0 6 15 178 7 0.0 6 7 179 3 0.0 6 3 180 10 0.0 6 10 181 5 0.0 6 5 182 8 0.0 6 8 183 12 0.0 6 12 184 10 0.0 6 10 185 9 0.0 6 9 186 8 0.0 6 8 187 9 0.0 6 9 188 9 0.0 6 9 189 7 0.0 6 7 190 13 0.0 6 13 191 9 0.0 6 9 192 7 0.0 6 7 193 7 0.0 6 7 194 5 0.0 6 5 195 10 0.0 6 10 196 9 0.0 6 9 197 10 0.0 6 10 198 4 0.0 6 4 199 11 0.0 6 11 200 8 0.0 6 8 201 12 0.0 6 12 202 9 0.0 6 9 203 7 0.0 6 7 204 5 0.0 6 5 205 6 0.0 6 6 206 6 0.0 6 6 207 8 0.0 6 8 208 3 0.0 6 3 209 3 0.0 6 3 210 8 0.0 6 8 211 5 0.0 6 5 212 4 0.0 6 4 213 7 0.0 6 7 214 8 0.0 6 8 215 5 0.0 6 5 216 7 0.0 6 7 217 8 0.0 6 8 218 4 0.0 6 4 219 6 0.0 6 6 220 3 0.0 6 3 221 5 0.0 6 5 222 7 0.0 6 7 223 10 0.0 6 10 224 2 0.0 6 2 225 7 0.0 6 7 226 5 0.0 6 5 227 5 0.0 6 5 228 6 0.0 6 6 229 2 0.0 6 2 230 2 0.0 6 2 231 4 0.0 6 4 232 1 0.0 6 1 233 4 0.0 6 4 234 3 0.0 6 3 235 8 0.0 6 8 236 5 0.0 6 5 237 4 0.0 6 4 238 3 0.0 6 3 239 5 0.0 6 5 240 6 0.0 6 6 241 6 0.0 6 6 242 2 0.0 6 2 243 4 0.0 6 4 244 4 0.0 6 4 245 4 0.0 6 4 246 4 0.0 6 4 247 3 0.0 6 3 248 4 0.0 6 4 249 3 0.0 6 3 250 6 0.0 6 6 251 5 0.0 6 5 252 5 0.0 6 5 253 1 0.0 6 1 254 3 0.0 6 3 255 2 0.0 6 2 256 5 0.0 6 5 257 2 0.0 6 2 258 5 0.0 6 5 259 1 0.0 6 1 260 3 0.0 6 3 262 4 0.0 6 4 264 1 0.0 6 1 266 1 0.0 6 1 267 1 0.0 6 1 268 2 0.0 6 2 269 1 0.0 6 1 271 2 0.0 6 2 278 2 0.0 6 2 279 2 0.0 6 2 280 1 0.0 6 1 282 1 0.0 6 1 287 2 0.0 6 2 289 2 0.0 6 2 291 1 0.0 6 1 294 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 74834 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 9 2 0.3 2 2 11 2 0.0 3 2 12 7 0.0 3 7 13 15 0.0 3 10 4 0 1 14 10 0.0 4 10 15 2 0.0 4 1 0 0 1 16 18 0.0 4 17 1 17 24 0.0 5 23 1 18 18 0.0 5 18 19 22 0.0 5 21 1 20 139 0.0 6 136 3 21 79 0.0 6 76 2 0 1 22 51 0.0 6 48 3 23 39 0.0 6 38 1 24 39 0.0 6 38 1 25 33 0.0 6 33 26 25 0.0 6 24 1 27 24 0.0 6 24 28 17 0.0 6 17 29 12 0.0 6 10 2 30 37 0.0 6 34 1 1 0 0 1 31 19 0.0 6 19 32 24 0.0 6 24 33 14 0.0 6 12 2 34 14 0.0 6 14 35 15 0.0 6 13 2 36 19 0.0 6 18 1 37 13 0.0 6 13 38 24 0.0 6 22 1 1 39 6 0.0 6 5 1 40 15 0.0 6 15 41 18 0.0 6 18 42 17 0.0 6 16 1 43 17 0.0 6 14 2 0 0 0 0 1 44 19 0.0 6 17 2 45 15 0.0 6 14 1 46 22 0.0 6 22 47 19 0.0 6 18 1 48 20 0.0 6 20 49 16 0.0 6 15 1 50 29 0.0 6 27 2 51 29 0.0 6 28 1 52 26 0.0 6 26 53 41 0.0 6 40 1 54 28 0.0 6 24 4 55 37 0.0 6 32 4 0 0 1 56 19 0.0 6 18 1 57 33 0.0 6 28 5 58 28 0.0 6 28 59 36 0.0 6 34 2 60 40 0.0 6 37 2 0 0 0 0 1 61 21 0.0 6 19 0 0 1 0 0 1 62 30 0.0 6 29 1 63 30 0.0 6 27 2 1 64 26 0.0 6 25 1 65 36 0.0 6 34 1 0 1 66 40 0.0 6 38 2 67 30 0.0 6 28 2 68 61 0.0 6 61 69 146 0.0 6 136 7 0 3 70 68 0.0 6 64 3 0 1 71 80 0.0 6 72 7 1 72 58 0.0 6 53 5 73 54 0.0 6 53 1 74 40 0.0 6 38 2 75 35 0.0 6 32 3 76 43 0.0 6 39 4 77 53 0.0 6 52 0 0 0 1 78 55 0.0 6 53 1 1 79 65 0.0 6 62 3 80 50 0.0 6 46 3 1 81 64 0.0 6 61 2 1 82 73 0.0 6 70 2 0 1 83 62 0.0 6 60 1 0 0 1 84 79 0.0 6 78 1 85 93 0.0 6 90 2 0 0 1 86 59 0.0 6 57 2 87 58 0.0 6 56 2 88 54 0.0 6 53 1 89 51 0.0 6 47 4 90 67 0.0 6 63 3 0 0 1 91 66 0.0 6 61 4 0 1 92 80 0.0 6 78 2 93 103 0.0 6 98 4 0 1 94 91 0.0 6 83 7 0 0 1 95 83 0.0 6 78 4 0 1 96 93 0.0 6 92 1 97 96 0.0 6 94 2 98 98 0.0 6 95 2 0 0 1 99 108 0.0 6 104 4 100 100 0.0 6 98 2 101 86 0.0 6 81 4 1 102 96 0.0 6 94 2 103 107 0.0 6 99 7 1 104 116 0.0 6 113 3 105 140 0.0 6 132 5 2 0 0 0 1 106 215 0.0 6 211 4 107 129 0.0 6 124 5 108 123 0.0 6 117 6 109 148 0.0 6 138 8 1 0 0 0 1 110 135 0.0 6 131 2 2 111 132 0.0 6 127 4 0 0 0 1 112 170 0.0 6 162 8 113 161 0.0 6 160 1 114 181 0.0 6 178 2 0 1 115 172 0.0 6 164 8 116 173 0.0 6 170 3 117 167 0.0 6 161 5 0 0 0 0 1 118 171 0.0 6 166 5 119 213 0.0 6 205 8 120 231 0.0 6 226 5 121 208 0.0 6 203 3 1 0 1 122 217 0.0 6 214 3 123 219 0.0 6 213 6 124 236 0.0 6 231 5 125 252 0.0 6 245 6 1 126 241 0.0 6 234 7 127 323 0.0 6 314 8 0 0 0 1 128 340 0.0 6 330 10 129 308 0.0 6 297 8 0 0 2 1 130 342 0.0 6 335 5 0 1 0 1 131 402 0.0 6 393 6 0 1 1 0 1 132 394 0.0 6 389 5 133 459 0.0 6 454 4 0 0 1 134 479 0.0 6 469 9 1 135 530 0.0 6 516 10 1 1 0 1 1 136 530 0.0 6 521 5 1 1 1 1 137 587 0.0 6 573 11 2 0 0 0 1 138 593 0.0 6 581 11 0 0 0 1 139 718 0.0 6 708 9 0 0 1 140 746 0.0 6 733 11 0 0 0 2 141 724 0.0 6 711 12 1 142 803 0.0 6 791 6 0 1 2 1 2 143 894 0.0 6 878 13 1 1 1 144 937 0.0 6 915 21 1 145 876 0.0 6 865 8 0 0 1 2 146 942 0.0 6 923 17 0 1 1 147 901 0.0 6 885 14 2 148 1035 0.0 6 1024 10 0 0 1 149 1037 0.0 6 1025 12 150 1151 0.0 6 1128 19 1 1 1 0 1 151 1116 0.0 6 1102 9 3 1 1 152 1706 0.0 6 1676 23 2 1 1 2 1 153 1187 0.0 6 1171 14 0 0 0 0 2 154 1469 0.0 6 1440 25 1 0 2 1 155 1376 0.0 6 1352 24 156 1179 0.0 6 1164 13 0 0 1 0 1 157 1295 0.0 6 1274 20 0 0 1 158 1154 0.0 6 1131 22 0 0 1 159 1105 0.0 6 1094 9 1 0 1 160 1204 0.0 6 1177 21 3 1 1 0 1 161 1090 0.0 6 1070 17 1 0 1 1 162 1010 0.0 6 990 14 3 0 2 1 163 959 0.0 6 939 16 1 1 1 0 1 164 872 0.0 6 858 10 0 1 0 0 3 165 902 0.0 6 886 13 2 0 0 0 1 166 955 0.0 6 941 10 2 1 1 167 808 0.0 6 797 10 0 1 168 1121 0.0 6 1103 16 1 0 0 0 1 169 803 0.0 6 788 11 1 1 1 1 170 1019 0.0 6 1005 13 0 1 171 1016 0.0 6 1007 8 0 0 0 1 172 776 0.0 6 765 11 173 907 0.0 6 894 11 0 0 1 0 1 174 985 0.0 6 980 5 175 1045 0.0 6 1031 13 0 0 0 0 1 176 955 0.0 6 939 13 1 0 1 1 177 929 0.0 6 908 17 0 2 0 1 1 178 810 0.0 6 796 11 1 2 179 773 0.0 6 765 7 0 0 0 1 180 725 0.0 6 716 6 2 0 0 0 1 181 665 0.0 6 658 6 0 1 182 530 0.0 6 524 6 183 627 0.0 6 619 6 0 1 0 0 1 184 421 0.0 6 417 3 0 0 1 185 750 0.0 6 744 6 186 1145 0.0 6 1123 20 2 187 956 0.0 6 944 9 1 0 1 0 1 188 818 0.0 6 805 12 1 189 720 0.0 6 709 11 190 814 0.0 6 799 15 191 723 0.0 6 711 11 0 0 1 192 598 0.0 6 585 12 0 0 1 193 500 0.0 6 487 12 0 0 1 194 562 0.0 6 544 14 2 0 0 1 1 195 627 0.0 6 610 16 1 196 974 0.0 6 948 23 2 0 0 0 1 197 336 0.0 6 324 10 0 1 1 198 854 0.0 6 832 20 0 1 1 199 1082 0.0 6 1055 24 0 1 2 200 357 0.0 6 350 6 1 201 442 0.0 6 435 7 202 287 0.0 6 282 5 203 273 0.0 6 260 13 204 371 0.0 6 361 10 205 185 0.0 6 182 2 1 206 163 0.0 6 161 1 0 0 0 0 1 207 129 0.0 6 127 1 1 208 166 0.0 6 162 4 209 140 0.0 6 137 3 210 133 0.0 6 127 6 211 168 0.0 6 162 4 1 1 212 126 0.0 6 122 4 213 75 0.0 6 74 1 214 89 0.0 6 88 1 215 109 0.0 6 108 1 216 253 0.0 6 249 3 1 217 166 0.0 6 160 6 218 104 0.0 6 99 4 0 1 219 87 0.0 6 86 1 220 99 0.0 6 97 2 221 84 0.0 6 83 1 222 131 0.0 6 128 3 223 367 0.0 6 360 7 224 52 0.0 6 51 1 225 94 0.0 6 90 3 0 0 0 0 1 226 65 0.0 6 62 3 227 52 0.0 6 52 228 47 0.0 6 46 1 229 71 0.0 6 71 230 28 0.0 6 28 231 34 0.0 6 34 232 62 0.0 6 60 2 233 84 0.0 6 82 2 234 30 0.0 6 27 3 235 15 0.0 6 15 236 15 0.0 6 14 1 237 9 0.0 6 9 238 5 0.0 6 4 1 239 12 0.0 6 12 240 21 0.0 6 21 241 18 0.0 6 18 242 40 0.0 6 40 243 40 0.0 6 40 244 19 0.0 6 19 245 12 0.0 6 12 246 4 0.0 6 4 247 3 0.0 6 3 249 3 0.0 6 3 250 5 0.0 6 4 1 251 5 0.0 6 5 252 1 0.0 6 0 1 253 2 0.0 6 2 254 1 0.0 6 1 255 1 0.0 6 1 257 1 0.0 6 1 261 1 0.0 6 1 278 2 0.0 6 2 279 8 0.0 6 8 280 21 0.0 6 20 1 281 117 0.0 6 114 3 283 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 937 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 47 3 0.0 6 0 3 52 1 0.0 6 1 60 1 0.0 6 1 63 1 0.0 6 1 67 2 0.0 6 2 68 2 0.0 6 2 69 1 0.0 6 1 70 5 0.0 6 5 71 7 0.0 6 7 72 21 0.0 6 21 73 5 0.0 6 5 74 6 0.0 6 6 75 7 0.0 6 6 1 76 9 0.0 6 9 77 3 0.0 6 3 78 10 0.0 6 10 79 9 0.0 6 9 80 9 0.0 6 9 81 10 0.0 6 10 82 5 0.0 6 5 83 6 0.0 6 5 1 84 6 0.0 6 6 85 7 0.0 6 6 0 0 0 1 86 8 0.0 6 8 87 10 0.0 6 10 88 7 0.0 6 7 89 7 0.0 6 7 90 11 0.0 6 11 91 7 0.0 6 7 92 6 0.0 6 6 93 7 0.0 6 7 94 3 0.0 6 3 95 5 0.0 6 5 96 5 0.0 6 5 97 9 0.0 6 9 98 4 0.0 6 4 99 7 0.0 6 7 100 3 0.0 6 3 101 8 0.0 6 8 102 9 0.0 6 9 103 10 0.0 6 10 104 6 0.0 6 6 105 5 0.0 6 5 106 4 0.0 6 4 107 9 0.0 6 9 108 14 0.0 6 14 109 5 0.0 6 4 1 110 5 0.0 6 4 1 111 7 0.0 6 7 112 7 0.0 6 7 113 6 0.0 6 6 114 5 0.0 6 5 115 11 0.0 6 10 0 0 0 1 116 8 0.0 6 8 117 5 0.0 6 5 118 3 0.0 6 3 119 3 0.0 6 3 120 2 0.0 6 2 121 4 0.0 6 4 122 5 0.0 6 5 123 4 0.0 6 4 124 3 0.0 6 3 125 6 0.0 6 6 126 9 0.0 6 9 127 7 0.0 6 7 128 4 0.0 6 4 129 4 0.0 6 4 130 7 0.0 6 7 131 4 0.0 6 4 132 11 0.0 6 9 0 0 1 1 133 8 0.0 6 8 134 6 0.0 6 6 135 11 0.0 6 10 0 0 0 1 136 6 0.0 6 5 1 137 11 0.0 6 11 138 11 0.0 6 11 139 5 0.0 6 5 140 13 0.0 6 13 141 8 0.0 6 8 142 7 0.0 6 7 143 3 0.0 6 3 144 9 0.0 6 9 145 6 0.0 6 6 146 3 0.0 6 3 147 6 0.0 6 6 148 6 0.0 6 6 149 10 0.0 6 10 150 5 0.0 6 5 151 5 0.0 6 5 152 5 0.0 6 5 153 5 0.0 6 5 154 11 0.0 6 11 155 6 0.0 6 6 156 16 0.0 6 16 157 6 0.0 6 6 158 3 0.0 6 3 159 14 0.0 6 14 160 7 0.0 6 7 161 12 0.0 6 12 162 11 0.0 6 11 163 11 0.0 6 11 164 9 0.0 6 9 165 16 0.0 6 16 166 5 0.0 6 5 167 5 0.0 6 5 168 10 0.0 6 10 169 2 0.0 6 2 170 3 0.0 6 3 171 12 0.0 6 11 0 0 0 1 172 9 0.0 6 9 173 4 0.0 6 4 174 7 0.0 6 7 175 8 0.0 6 8 176 5 0.0 6 4 0 0 1 177 10 0.0 6 10 178 10 0.0 6 10 179 2 0.0 6 2 180 9 0.0 6 9 181 7 0.0 6 7 182 3 0.0 6 3 183 6 0.0 6 6 184 5 0.0 6 4 0 1 185 8 0.0 6 8 186 5 0.0 6 5 187 4 0.0 6 4 188 8 0.0 6 8 189 1 0.0 6 1 190 4 0.0 6 4 191 11 0.0 6 11 192 5 0.0 6 5 193 2 0.0 6 2 194 6 0.0 6 6 195 5 0.0 6 5 196 4 0.0 6 4 197 3 0.0 6 3 198 1 0.0 6 1 199 5 0.0 6 5 200 3 0.0 6 3 201 6 0.0 6 6 202 3 0.0 6 3 203 1 0.0 6 1 204 1 0.0 6 1 205 3 0.0 6 3 209 3 0.0 6 3 210 2 0.0 6 2 211 3 0.0 6 3 213 1 0.0 6 1 214 1 0.0 6 1 216 3 0.0 6 3 217 1 0.0 6 1 219 1 0.0 6 1 220 1 0.0 6 1 226 1 0.0 6 1 231 1 0.0 6 1 235 1 0.0 6 1 246 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_06_89_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_06_316_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_06_89_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_06_316_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.05 s (26 µs/read; 2.27 M reads/minute). === Summary === Total read pairs processed: 77,650 Read 1 with adapter: 77,650 (100.0%) Read 2 with adapter: 77,619 (100.0%) == Read fate breakdown == Pairs that were too short: 16 (0.0%) Pairs discarded as untrimmed: 31 (0.0%) Pairs written (passing filters): 77,603 (99.9%) Total basepairs processed: 36,538,322 bp Read 1: 21,004,070 bp Read 2: 15,534,252 bp Total written (filtered): 4,621,947 bp (12.6%) Read 1: 2,357,472 bp Read 2: 2,264,475 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 76904 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 19.0 1 0 0 1 7 1 4.7 2 0 0 1 9 2 0.3 2 1 0 0 1 10 2 0.1 3 2 12 1 0.0 3 1 14 1 0.0 4 1 15 1 0.0 4 1 16 2 0.0 4 1 1 17 2 0.0 5 2 18 2 0.0 5 2 19 1 0.0 5 1 20 62 0.0 6 61 1 21 34 0.0 6 31 3 22 28 0.0 6 28 23 16 0.0 6 15 1 24 13 0.0 6 13 25 23 0.0 6 21 2 26 13 0.0 6 13 27 3 0.0 6 3 28 2 0.0 6 2 29 7 0.0 6 7 30 4 0.0 6 3 1 31 5 0.0 6 5 32 4 0.0 6 3 0 1 33 4 0.0 6 4 34 8 0.0 6 8 35 5 0.0 6 5 36 4 0.0 6 4 37 3 0.0 6 3 38 3 0.0 6 3 39 9 0.0 6 9 40 5 0.0 6 5 41 2 0.0 6 2 42 4 0.0 6 4 43 6 0.0 6 6 44 4 0.0 6 3 0 1 45 5 0.0 6 4 1 46 4 0.0 6 4 47 2 0.0 6 2 48 1 0.0 6 1 49 6 0.0 6 6 50 4 0.0 6 4 51 5 0.0 6 5 52 6 0.0 6 5 1 53 4 0.0 6 4 54 4 0.0 6 4 55 5 0.0 6 5 56 6 0.0 6 5 1 57 5 0.0 6 5 58 9 0.0 6 9 59 4 0.0 6 4 60 4 0.0 6 3 0 0 0 1 61 4 0.0 6 4 62 5 0.0 6 5 63 7 0.0 6 7 64 3 0.0 6 3 65 7 0.0 6 5 2 66 5 0.0 6 5 67 5 0.0 6 5 68 2 0.0 6 2 69 4 0.0 6 4 70 6 0.0 6 6 71 9 0.0 6 9 72 6 0.0 6 6 73 6 0.0 6 6 74 5 0.0 6 5 75 8 0.0 6 8 76 2 0.0 6 2 77 10 0.0 6 9 1 78 6 0.0 6 6 79 12 0.0 6 11 1 80 3 0.0 6 3 81 7 0.0 6 7 82 6 0.0 6 6 83 13 0.0 6 10 2 1 84 7 0.0 6 6 1 85 6 0.0 6 5 1 86 17 0.0 6 17 87 4 0.0 6 4 88 9 0.0 6 8 0 0 0 0 1 89 10 0.0 6 10 90 5 0.0 6 5 91 5 0.0 6 3 1 0 0 0 0 1 92 5 0.0 6 5 93 4 0.0 6 4 94 5 0.0 6 4 0 1 95 3 0.0 6 3 96 7 0.0 6 7 97 9 0.0 6 9 98 9 0.0 6 7 2 99 6 0.0 6 5 1 100 8 0.0 6 8 101 7 0.0 6 7 102 15 0.0 6 15 103 9 0.0 6 9 104 7 0.0 6 7 105 13 0.0 6 13 106 8 0.0 6 6 2 107 17 0.0 6 16 1 108 8 0.0 6 8 109 13 0.0 6 11 2 110 5 0.0 6 4 1 111 12 0.0 6 12 112 11 0.0 6 11 113 8 0.0 6 8 114 13 0.0 6 10 3 115 12 0.0 6 12 116 16 0.0 6 15 1 117 9 0.0 6 9 118 10 0.0 6 9 1 119 15 0.0 6 15 120 14 0.0 6 14 121 9 0.0 6 8 0 1 122 17 0.0 6 16 1 123 18 0.0 6 18 124 16 0.0 6 15 1 125 19 0.0 6 18 1 126 20 0.0 6 20 127 12 0.0 6 12 128 24 0.0 6 24 129 13 0.0 6 12 1 130 19 0.0 6 19 131 15 0.0 6 15 132 16 0.0 6 16 133 25 0.0 6 25 134 23 0.0 6 22 1 135 36 0.0 6 36 136 30 0.0 6 28 2 137 23 0.0 6 23 138 48 0.0 6 47 1 139 29 0.0 6 28 1 140 59 0.0 6 57 2 141 115 0.0 6 114 0 0 0 0 1 142 190 0.0 6 189 1 143 102 0.0 6 102 144 125 0.0 6 125 145 185 0.0 6 185 146 171 0.0 6 169 1 1 147 93 0.0 6 92 1 148 111 0.0 6 108 3 149 141 0.0 6 138 3 150 109 0.0 6 104 4 1 151 81 0.0 6 79 2 152 118 0.0 6 116 2 153 109 0.0 6 108 1 154 101 0.0 6 101 155 105 0.0 6 104 1 156 90 0.0 6 90 157 117 0.0 6 116 1 158 107 0.0 6 105 2 159 117 0.0 6 115 1 0 1 160 154 0.0 6 153 1 161 133 0.0 6 129 4 162 142 0.0 6 140 2 163 134 0.0 6 132 2 164 126 0.0 6 124 2 165 124 0.0 6 121 3 166 162 0.0 6 162 167 99 0.0 6 99 168 96 0.0 6 95 1 169 179 0.0 6 178 1 170 172 0.0 6 170 2 171 155 0.0 6 152 3 172 168 0.0 6 166 2 173 144 0.0 6 144 174 177 0.0 6 176 1 175 243 0.0 6 241 2 176 138 0.0 6 134 4 177 204 0.0 6 203 1 178 410 0.0 6 408 1 0 0 0 0 1 179 206 0.0 6 204 2 180 146 0.0 6 144 2 181 183 0.0 6 181 2 182 187 0.0 6 184 3 183 148 0.0 6 145 3 184 176 0.0 6 175 1 185 207 0.0 6 202 5 186 264 0.0 6 258 6 187 203 0.0 6 202 1 188 219 0.0 6 217 2 189 183 0.0 6 179 2 0 2 190 227 0.0 6 225 2 191 240 0.0 6 238 2 192 268 0.0 6 260 8 193 323 0.0 6 318 5 194 287 0.0 6 279 8 195 309 0.0 6 306 3 196 405 0.0 6 397 6 2 197 375 0.0 6 369 6 198 435 0.0 6 427 7 0 0 0 1 199 491 0.0 6 483 7 1 200 483 0.0 6 479 4 201 449 0.0 6 445 4 202 470 0.0 6 464 4 1 0 1 203 483 0.0 6 472 10 1 204 429 0.0 6 423 6 205 475 0.0 6 471 3 1 206 397 0.0 6 385 12 207 524 0.0 6 514 9 0 0 0 1 208 640 0.0 6 630 9 0 0 0 0 1 209 452 0.0 6 442 10 210 477 0.0 6 466 9 1 0 1 211 359 0.0 6 350 8 1 212 315 0.0 6 311 4 213 543 0.0 6 538 4 0 0 0 0 1 214 563 0.0 6 552 11 215 567 0.0 6 562 5 216 571 0.0 6 559 10 1 0 0 1 217 782 0.0 6 772 9 1 218 558 0.0 6 544 11 2 0 0 1 219 582 0.0 6 575 6 1 220 567 0.0 6 559 8 221 505 0.0 6 499 6 222 520 0.0 6 513 5 0 0 0 0 2 223 520 0.0 6 511 8 1 224 433 0.0 6 427 6 225 463 0.0 6 453 10 226 624 0.0 6 614 9 0 0 0 1 227 674 0.0 6 665 8 1 228 753 0.0 6 743 9 1 229 585 0.0 6 576 9 230 633 0.0 6 626 7 231 511 0.0 6 503 7 1 232 596 0.0 6 593 3 233 621 0.0 6 611 10 234 615 0.0 6 607 8 235 562 0.0 6 557 4 1 236 488 0.0 6 482 6 237 474 0.0 6 466 8 238 514 0.0 6 507 7 239 454 0.0 6 444 10 240 544 0.0 6 533 9 1 0 1 241 642 0.0 6 631 11 242 572 0.0 6 564 7 0 1 243 544 0.0 6 537 7 244 523 0.0 6 511 11 1 245 568 0.0 6 556 11 0 0 0 0 1 246 334 0.0 6 327 6 1 247 384 0.0 6 381 3 248 310 0.0 6 301 9 249 457 0.0 6 447 10 250 366 0.0 6 361 5 251 407 0.0 6 397 9 0 0 0 1 252 402 0.0 6 388 13 1 253 345 0.0 6 335 10 254 514 0.0 6 500 14 255 389 0.0 6 382 6 1 256 421 0.0 6 413 7 0 0 0 0 1 257 349 0.0 6 343 6 258 367 0.0 6 353 14 259 494 0.0 6 480 13 1 260 627 0.0 6 610 16 1 261 363 0.0 6 353 10 262 443 0.0 6 424 19 263 555 0.0 6 544 9 1 1 264 450 0.0 6 434 16 265 459 0.0 6 456 3 266 330 0.0 6 322 8 267 434 0.0 6 422 11 1 268 410 0.0 6 392 18 269 457 0.0 6 446 10 1 270 277 0.0 6 264 13 271 554 0.0 6 523 29 2 272 860 0.0 6 840 18 2 273 493 0.0 6 485 7 1 274 576 0.0 6 562 13 1 275 732 0.0 6 711 19 2 276 1036 0.0 6 1015 20 1 277 1020 0.0 6 999 21 278 793 0.0 6 776 16 1 279 1351 0.0 6 1327 22 2 280 2355 0.0 6 2259 91 3 1 0 1 281 19903 0.0 6 19536 361 3 2 1 282 8 0.0 6 7 1 283 5 0.0 6 5 284 3 0.0 6 1 0 1 1 285 2 0.0 6 2 287 1 0.0 6 1 291 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 746 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 10 303.3 1 10 61 10 0.0 6 10 70 3 0.0 6 3 71 5 0.0 6 5 72 2 0.0 6 2 73 2 0.0 6 2 74 4 0.0 6 4 75 3 0.0 6 3 76 3 0.0 6 3 77 2 0.0 6 2 78 2 0.0 6 2 79 6 0.0 6 6 80 9 0.0 6 9 81 1 0.0 6 1 82 1 0.0 6 1 83 1 0.0 6 1 84 2 0.0 6 2 85 2 0.0 6 2 86 2 0.0 6 2 87 4 0.0 6 4 89 2 0.0 6 2 90 4 0.0 6 4 91 2 0.0 6 2 92 4 0.0 6 4 93 1 0.0 6 1 94 3 0.0 6 3 95 4 0.0 6 4 96 3 0.0 6 3 97 2 0.0 6 2 99 2 0.0 6 2 100 1 0.0 6 1 101 3 0.0 6 3 102 5 0.0 6 5 103 1 0.0 6 1 104 4 0.0 6 4 105 3 0.0 6 3 106 1 0.0 6 1 107 3 0.0 6 3 108 12 0.0 6 12 109 3 0.0 6 3 110 2 0.0 6 2 111 2 0.0 6 2 112 3 0.0 6 3 115 1 0.0 6 1 116 2 0.0 6 2 118 1 0.0 6 1 119 2 0.0 6 2 120 3 0.0 6 3 121 2 0.0 6 2 122 5 0.0 6 5 124 2 0.0 6 2 125 3 0.0 6 3 126 4 0.0 6 4 127 5 0.0 6 5 128 4 0.0 6 4 129 1 0.0 6 1 130 2 0.0 6 2 131 3 0.0 6 3 132 6 0.0 6 6 133 5 0.0 6 5 135 1 0.0 6 1 136 2 0.0 6 2 137 1 0.0 6 1 138 2 0.0 6 2 139 3 0.0 6 3 140 4 0.0 6 4 141 2 0.0 6 2 142 1 0.0 6 1 143 2 0.0 6 2 144 4 0.0 6 4 145 3 0.0 6 3 146 3 0.0 6 3 147 1 0.0 6 1 148 4 0.0 6 4 149 5 0.0 6 5 150 2 0.0 6 2 151 2 0.0 6 2 152 2 0.0 6 2 153 6 0.0 6 6 154 6 0.0 6 6 155 8 0.0 6 8 156 4 0.0 6 4 157 3 0.0 6 3 158 2 0.0 6 2 159 2 0.0 6 2 160 4 0.0 6 4 161 4 0.0 6 4 162 8 0.0 6 8 163 5 0.0 6 5 164 6 0.0 6 6 165 3 0.0 6 3 166 3 0.0 6 3 167 4 0.0 6 4 168 5 0.0 6 5 169 7 0.0 6 7 170 9 0.0 6 9 171 9 0.0 6 9 172 8 0.0 6 8 173 5 0.0 6 5 174 8 0.0 6 8 175 2 0.0 6 2 176 5 0.0 6 5 178 6 0.0 6 6 179 2 0.0 6 2 180 4 0.0 6 4 181 3 0.0 6 3 182 1 0.0 6 1 183 8 0.0 6 8 184 1 0.0 6 1 185 7 0.0 6 7 186 6 0.0 6 6 187 4 0.0 6 4 188 4 0.0 6 4 189 6 0.0 6 6 190 5 0.0 6 5 191 9 0.0 6 9 192 2 0.0 6 2 193 4 0.0 6 4 194 3 0.0 6 3 195 4 0.0 6 4 196 4 0.0 6 4 197 2 0.0 6 2 198 3 0.0 6 3 199 5 0.0 6 5 200 8 0.0 6 8 201 12 0.0 6 12 202 2 0.0 6 2 203 7 0.0 6 7 204 6 0.0 6 6 205 7 0.0 6 7 206 11 0.0 6 11 207 10 0.0 6 10 208 6 0.0 6 6 209 7 0.0 6 7 210 11 0.0 6 11 211 6 0.0 6 6 212 1 0.0 6 1 213 4 0.0 6 4 214 8 0.0 6 8 215 1 0.0 6 1 216 6 0.0 6 6 217 2 0.0 6 2 218 4 0.0 6 4 219 5 0.0 6 5 220 5 0.0 6 5 221 3 0.0 6 3 222 2 0.0 6 2 223 7 0.0 6 7 224 2 0.0 6 2 225 5 0.0 6 5 226 5 0.0 6 5 227 4 0.0 6 4 228 5 0.0 6 5 229 2 0.0 6 2 230 4 0.0 6 4 231 5 0.0 6 5 232 5 0.0 6 5 233 5 0.0 6 5 234 3 0.0 6 3 235 3 0.0 6 3 236 3 0.0 6 3 237 1 0.0 6 1 238 6 0.0 6 6 239 3 0.0 6 3 241 3 0.0 6 3 242 3 0.0 6 3 243 3 0.0 6 3 245 5 0.0 6 5 246 6 0.0 6 5 1 247 2 0.0 6 2 248 4 0.0 6 4 249 1 0.0 6 1 250 3 0.0 6 3 251 2 0.0 6 2 252 4 0.0 6 4 253 3 0.0 6 3 254 1 0.0 6 1 255 3 0.0 6 3 257 2 0.0 6 2 258 4 0.0 6 4 260 2 0.0 6 2 262 1 0.0 6 1 263 1 0.0 6 1 266 2 0.0 6 2 267 2 0.0 6 2 269 1 0.0 6 1 270 4 0.0 6 4 271 5 0.0 6 5 272 2 0.0 6 2 273 2 0.0 6 2 274 2 0.0 6 2 275 1 0.0 6 1 276 1 0.0 6 1 278 2 0.0 6 2 280 1 0.0 6 1 282 1 0.0 6 1 283 2 0.0 6 2 286 1 0.0 6 1 289 1 0.0 6 1 291 1 0.0 6 1 292 1 0.0 6 1 294 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 77114 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 19.0 1 1 10 2 0.1 3 1 1 11 1 0.0 3 1 12 4 0.0 3 4 13 6 0.0 3 5 0 1 14 13 0.0 4 12 1 15 1 0.0 4 1 16 8 0.0 4 7 1 17 26 0.0 5 23 3 18 4 0.0 5 3 1 19 65 0.0 5 59 6 20 117 0.0 6 112 5 21 84 0.0 6 83 1 22 39 0.0 6 38 1 23 30 0.0 6 23 6 1 24 29 0.0 6 28 0 1 25 22 0.0 6 18 4 26 24 0.0 6 21 2 0 1 27 26 0.0 6 23 2 1 28 15 0.0 6 12 3 29 27 0.0 6 22 5 30 22 0.0 6 19 3 31 22 0.0 6 22 32 15 0.0 6 13 2 33 20 0.0 6 16 2 1 0 1 34 16 0.0 6 15 1 35 9 0.0 6 9 36 17 0.0 6 17 37 13 0.0 6 11 1 1 38 13 0.0 6 13 39 19 0.0 6 18 0 0 0 1 40 10 0.0 6 8 2 41 15 0.0 6 13 1 0 1 42 17 0.0 6 14 3 43 16 0.0 6 13 2 1 44 11 0.0 6 11 45 12 0.0 6 11 1 46 24 0.0 6 21 3 47 22 0.0 6 22 48 14 0.0 6 12 1 1 49 20 0.0 6 19 1 50 26 0.0 6 24 1 1 51 25 0.0 6 24 1 52 32 0.0 6 27 3 1 0 1 53 20 0.0 6 18 2 54 29 0.0 6 25 3 0 0 1 55 25 0.0 6 22 3 56 36 0.0 6 29 5 2 57 18 0.0 6 17 1 58 28 0.0 6 25 3 59 35 0.0 6 31 4 60 24 0.0 6 21 3 61 22 0.0 6 20 1 0 0 0 1 62 31 0.0 6 30 1 63 19 0.0 6 16 2 0 0 1 64 21 0.0 6 20 1 65 23 0.0 6 21 1 1 66 24 0.0 6 24 67 35 0.0 6 34 0 1 68 33 0.0 6 30 3 69 45 0.0 6 41 2 2 70 142 0.0 6 139 2 0 0 0 1 71 54 0.0 6 48 6 72 83 0.0 6 82 1 73 33 0.0 6 27 6 74 53 0.0 6 50 3 75 36 0.0 6 35 0 1 76 36 0.0 6 35 1 77 47 0.0 6 42 4 0 1 78 43 0.0 6 40 3 79 28 0.0 6 27 1 80 29 0.0 6 27 1 1 81 32 0.0 6 30 2 82 47 0.0 6 44 2 0 0 1 83 62 0.0 6 58 3 0 0 1 84 62 0.0 6 59 2 1 85 49 0.0 6 45 3 0 1 86 82 0.0 6 76 6 87 56 0.0 6 49 7 88 46 0.0 6 45 1 89 38 0.0 6 34 4 90 63 0.0 6 56 5 1 1 91 59 0.0 6 54 3 1 1 92 54 0.0 6 50 2 1 1 93 75 0.0 6 69 6 94 109 0.0 6 99 5 3 2 95 70 0.0 6 66 3 1 96 88 0.0 6 83 3 0 0 0 1 1 97 65 0.0 6 61 3 1 98 62 0.0 6 57 3 1 0 0 1 99 89 0.0 6 83 6 100 76 0.0 6 70 5 0 0 0 1 101 81 0.0 6 78 3 102 93 0.0 6 82 8 1 1 1 103 106 0.0 6 103 3 104 96 0.0 6 91 3 1 0 1 105 90 0.0 6 87 2 1 106 91 0.0 6 90 1 107 123 0.0 6 115 8 108 85 0.0 6 84 1 109 86 0.0 6 85 1 110 97 0.0 6 92 5 111 88 0.0 6 83 5 112 100 0.0 6 96 4 113 97 0.0 6 93 3 1 114 111 0.0 6 105 6 115 150 0.0 6 140 10 116 120 0.0 6 114 6 117 112 0.0 6 108 4 118 120 0.0 6 117 3 119 122 0.0 6 120 1 0 0 0 1 120 165 0.0 6 161 3 0 0 1 121 151 0.0 6 146 5 122 136 0.0 6 133 3 123 161 0.0 6 157 3 0 0 1 124 222 0.0 6 212 8 0 1 0 0 1 125 155 0.0 6 152 3 126 153 0.0 6 151 1 0 0 0 0 1 127 211 0.0 6 206 4 1 128 203 0.0 6 195 5 1 1 0 0 1 129 243 0.0 6 236 7 130 194 0.0 6 186 7 0 1 131 244 0.0 6 239 5 132 324 0.0 6 315 8 0 0 1 133 328 0.0 6 321 7 134 345 0.0 6 331 9 1 1 3 135 400 0.0 6 388 9 0 1 1 1 136 384 0.0 6 371 11 0 0 2 137 425 0.0 6 415 10 138 424 0.0 6 407 14 0 0 1 0 2 139 411 0.0 6 401 9 1 140 484 0.0 6 475 7 0 0 0 1 1 141 456 0.0 6 445 7 2 2 142 539 0.0 6 524 14 0 0 0 1 143 553 0.0 6 539 13 0 0 1 144 647 0.0 6 630 15 0 0 1 0 1 145 567 0.0 6 553 11 0 0 1 1 1 146 507 0.0 6 496 11 147 511 0.0 6 500 9 1 0 0 0 1 148 547 0.0 6 533 10 2 0 0 1 1 149 698 0.0 6 673 21 1 0 2 1 150 797 0.0 6 770 21 2 0 2 2 151 751 0.0 6 733 17 0 0 1 152 966 0.0 6 948 15 2 0 1 153 1093 0.0 6 1066 26 0 0 0 0 1 154 856 0.0 6 836 17 0 1 2 155 1066 0.0 6 1035 27 1 2 0 0 1 156 1033 0.0 6 1004 26 0 0 2 0 1 157 886 0.0 6 855 29 0 1 1 158 1152 0.0 6 1125 24 0 3 159 747 0.0 6 735 11 0 1 160 997 0.0 6 975 19 0 0 0 2 1 161 825 0.0 6 804 18 2 1 162 717 0.0 6 703 14 163 833 0.0 6 819 13 0 0 0 1 164 768 0.0 6 752 11 2 0 2 0 1 165 777 0.0 6 761 10 1 1 1 2 1 166 990 0.0 6 969 17 0 1 0 0 3 167 610 0.0 6 597 10 1 0 1 0 1 168 946 0.0 6 927 19 169 708 0.0 6 698 9 0 0 0 0 1 170 639 0.0 6 622 14 1 1 0 1 171 966 0.0 6 946 19 0 1 172 598 0.0 6 589 8 1 173 695 0.0 6 677 15 0 1 1 1 174 780 0.0 6 758 19 1 0 1 1 175 1158 0.0 6 1135 21 0 0 2 176 916 0.0 6 893 19 1 2 1 177 1003 0.0 6 982 19 0 0 0 2 178 688 0.0 6 678 10 179 942 0.0 6 923 18 1 180 836 0.0 6 815 19 0 2 181 1127 0.0 6 1099 27 0 0 0 1 182 552 0.0 6 529 20 0 1 0 1 1 183 852 0.0 6 835 14 1 0 2 184 432 0.0 6 419 12 0 1 185 416 0.0 6 403 12 1 186 1050 0.0 6 1022 28 187 1287 0.0 6 1249 33 1 2 1 1 188 1358 0.0 6 1324 30 1 1 2 189 1076 0.0 6 1051 25 190 1534 0.0 6 1496 37 1 191 1258 0.0 6 1225 32 1 192 998 0.0 6 973 22 1 1 1 193 744 0.0 6 730 13 0 0 0 1 194 978 0.0 6 955 22 1 195 1049 0.0 6 1006 42 0 0 0 1 196 2341 0.0 6 2289 51 0 0 1 197 791 0.0 6 771 19 0 0 0 1 198 1342 0.0 6 1304 34 3 0 0 1 199 2699 0.0 6 2617 78 1 0 3 200 793 0.0 6 779 14 201 1010 0.0 6 988 22 202 594 0.0 6 576 17 1 203 513 0.0 6 496 17 204 535 0.0 6 526 9 205 350 0.0 6 341 8 0 0 1 206 318 0.0 6 310 6 1 0 0 1 207 212 0.0 6 208 3 0 0 1 208 312 0.0 6 305 6 0 0 1 209 325 0.0 6 314 10 0 0 1 210 312 0.0 6 301 8 2 1 211 425 0.0 6 410 15 212 468 0.0 6 454 14 213 152 0.0 6 150 1 0 0 0 0 1 214 321 0.0 6 321 215 190 0.0 6 185 4 0 0 1 216 453 0.0 6 442 10 1 217 513 0.0 6 500 13 218 247 0.0 6 244 3 219 261 0.0 6 253 8 220 205 0.0 6 201 4 221 294 0.0 6 283 11 222 528 0.0 6 515 12 0 0 1 223 519 0.0 6 506 13 224 156 0.0 6 151 5 225 275 0.0 6 270 5 226 156 0.0 6 153 3 227 198 0.0 6 194 4 228 106 0.0 6 102 4 229 183 0.0 6 176 6 0 0 1 230 100 0.0 6 100 231 107 0.0 6 106 1 232 162 0.0 6 159 3 233 154 0.0 6 153 1 234 87 0.0 6 85 2 235 74 0.0 6 72 2 236 26 0.0 6 25 1 237 46 0.0 6 45 1 238 18 0.0 6 18 239 20 0.0 6 20 240 39 0.0 6 39 241 77 0.0 6 74 3 242 114 0.0 6 107 7 243 60 0.0 6 59 1 244 155 0.0 6 153 2 245 40 0.0 6 38 2 246 32 0.0 6 32 247 14 0.0 6 13 1 248 15 0.0 6 15 249 22 0.0 6 21 1 250 13 0.0 6 13 251 25 0.0 6 25 252 9 0.0 6 9 253 6 0.0 6 5 1 254 10 0.0 6 10 255 1 0.0 6 1 256 2 0.0 6 2 279 3 0.0 6 2 1 280 4 0.0 6 3 1 281 17 0.0 6 17 282 69 0.0 6 67 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 505 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 4 0.0 6 4 70 4 0.0 6 4 71 7 0.0 6 7 72 4 0.0 6 4 73 8 0.0 6 8 74 9 0.0 6 9 75 4 0.0 6 4 76 6 0.0 6 6 77 4 0.0 6 4 78 2 0.0 6 2 79 1 0.0 6 1 80 1 0.0 6 1 81 1 0.0 6 1 82 4 0.0 6 4 83 4 0.0 6 4 84 2 0.0 6 2 85 2 0.0 6 2 86 2 0.0 6 2 87 2 0.0 6 2 88 4 0.0 6 4 89 4 0.0 6 4 90 1 0.0 6 1 91 6 0.0 6 6 92 1 0.0 6 1 93 1 0.0 6 1 94 1 0.0 6 1 95 4 0.0 6 4 97 2 0.0 6 2 98 3 0.0 6 3 99 5 0.0 6 5 100 5 0.0 6 5 101 3 0.0 6 3 102 2 0.0 6 2 103 3 0.0 6 2 0 0 1 104 7 0.0 6 7 106 6 0.0 6 6 107 16 0.0 6 16 108 8 0.0 6 8 109 2 0.0 6 2 110 4 0.0 6 4 111 4 0.0 6 4 112 2 0.0 6 2 113 2 0.0 6 2 114 3 0.0 6 3 115 2 0.0 6 2 116 1 0.0 6 1 117 3 0.0 6 3 118 4 0.0 6 4 119 1 0.0 6 1 120 1 0.0 6 1 121 6 0.0 6 5 1 122 4 0.0 6 4 123 6 0.0 6 6 124 4 0.0 6 4 125 1 0.0 6 1 126 4 0.0 6 4 127 5 0.0 6 5 128 1 0.0 6 1 129 2 0.0 6 2 130 4 0.0 6 4 131 9 0.0 6 9 132 5 0.0 6 5 133 3 0.0 6 3 134 5 0.0 6 5 135 2 0.0 6 2 136 3 0.0 6 3 137 4 0.0 6 4 138 1 0.0 6 1 139 6 0.0 6 6 140 6 0.0 6 5 0 0 1 141 2 0.0 6 2 142 2 0.0 6 2 143 3 0.0 6 3 144 5 0.0 6 5 145 3 0.0 6 3 146 3 0.0 6 3 147 7 0.0 6 7 148 4 0.0 6 4 149 2 0.0 6 2 150 4 0.0 6 4 152 4 0.0 6 4 153 1 0.0 6 1 154 4 0.0 6 4 155 3 0.0 6 3 156 3 0.0 6 2 0 0 1 157 2 0.0 6 2 158 3 0.0 6 3 159 4 0.0 6 4 160 2 0.0 6 2 161 12 0.0 6 12 162 3 0.0 6 3 163 3 0.0 6 3 164 8 0.0 6 8 165 2 0.0 6 2 166 6 0.0 6 6 167 7 0.0 6 7 168 3 0.0 6 3 169 2 0.0 6 2 170 12 0.0 6 12 171 7 0.0 6 7 172 5 0.0 6 5 173 1 0.0 6 1 174 1 0.0 6 1 175 4 0.0 6 4 176 2 0.0 6 2 177 2 0.0 6 2 178 4 0.0 6 4 179 6 0.0 6 6 180 4 0.0 6 4 181 3 0.0 6 3 182 2 0.0 6 2 183 1 0.0 6 1 184 5 0.0 6 5 185 5 0.0 6 5 186 5 0.0 6 5 187 2 0.0 6 2 188 6 0.0 6 6 189 9 0.0 6 9 190 2 0.0 6 2 191 2 0.0 6 2 193 3 0.0 6 3 194 6 0.0 6 6 195 5 0.0 6 5 196 5 0.0 6 4 1 197 1 0.0 6 1 198 4 0.0 6 4 199 3 0.0 6 3 200 2 0.0 6 2 202 1 0.0 6 1 203 4 0.0 6 4 207 1 0.0 6 1 208 3 0.0 6 3 209 2 0.0 6 2 210 1 0.0 6 1 212 1 0.0 6 1 224 1 0.0 6 1 225 1 0.0 6 1 233 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_15_90_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_15_317_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_15_90_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_15_317_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.74 s (24 µs/read; 2.48 M reads/minute). === Summary === Total read pairs processed: 113,166 Read 1 with adapter: 113,166 (100.0%) Read 2 with adapter: 113,101 (99.9%) == Read fate breakdown == Pairs that were too short: 50 (0.0%) Pairs discarded as untrimmed: 65 (0.1%) Pairs written (passing filters): 113,051 (99.9%) Total basepairs processed: 49,967,471 bp Read 1: 27,338,534 bp Read 2: 22,628,937 bp Total written (filtered): 7,311,039 bp (14.6%) Read 1: 3,572,681 bp Read 2: 3,738,358 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 110046 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 2 442.1 1 2 5 1 110.5 1 1 6 7 27.6 1 2 0 5 7 2 6.9 2 0 0 2 8 1 1.7 2 1 9 1 0.4 2 0 1 10 5 0.1 3 4 0 0 1 11 1 0.0 3 1 12 1 0.0 3 1 13 1 0.0 3 0 0 1 14 1 0.0 4 0 1 15 3 0.0 4 2 1 16 3 0.0 4 3 17 4 0.0 5 3 1 18 2 0.0 5 2 19 1 0.0 5 1 20 178 0.0 6 173 5 21 139 0.0 6 136 3 22 67 0.0 6 65 2 23 68 0.0 6 68 24 45 0.0 6 44 1 25 46 0.0 6 46 26 24 0.0 6 23 1 27 25 0.0 6 24 1 28 26 0.0 6 24 1 1 29 16 0.0 6 15 0 0 0 1 30 21 0.0 6 21 31 11 0.0 6 11 32 10 0.0 6 8 1 1 33 9 0.0 6 9 34 12 0.0 6 8 4 35 6 0.0 6 6 36 10 0.0 6 10 37 7 0.0 6 5 2 38 15 0.0 6 14 1 39 9 0.0 6 9 40 11 0.0 6 10 1 41 7 0.0 6 6 1 42 4 0.0 6 4 43 5 0.0 6 4 1 44 5 0.0 6 5 45 5 0.0 6 5 46 6 0.0 6 5 1 47 8 0.0 6 7 1 48 3 0.0 6 3 49 4 0.0 6 4 50 3 0.0 6 3 51 5 0.0 6 5 52 12 0.0 6 12 53 5 0.0 6 5 54 2 0.0 6 2 55 7 0.0 6 7 56 8 0.0 6 7 1 57 2 0.0 6 2 58 6 0.0 6 6 59 4 0.0 6 4 60 5 0.0 6 5 61 2 0.0 6 2 62 7 0.0 6 7 63 6 0.0 6 6 64 6 0.0 6 6 65 4 0.0 6 4 66 16 0.0 6 16 67 7 0.0 6 6 1 68 7 0.0 6 6 0 0 0 0 0 1 69 6 0.0 6 4 1 0 0 0 0 1 70 10 0.0 6 10 71 8 0.0 6 7 1 72 4 0.0 6 4 73 5 0.0 6 5 74 12 0.0 6 12 75 9 0.0 6 9 76 13 0.0 6 13 77 10 0.0 6 9 1 78 11 0.0 6 11 79 10 0.0 6 10 80 7 0.0 6 7 81 9 0.0 6 9 82 12 0.0 6 12 83 20 0.0 6 20 84 17 0.0 6 17 85 15 0.0 6 15 86 10 0.0 6 9 1 87 13 0.0 6 12 1 88 7 0.0 6 7 89 8 0.0 6 8 90 6 0.0 6 6 91 13 0.0 6 13 92 6 0.0 6 6 93 6 0.0 6 6 94 2 0.0 6 2 95 7 0.0 6 6 0 1 96 11 0.0 6 10 1 97 9 0.0 6 8 1 98 11 0.0 6 11 99 8 0.0 6 8 100 24 0.0 6 24 101 14 0.0 6 14 102 20 0.0 6 19 0 0 0 0 0 1 103 16 0.0 6 16 104 14 0.0 6 13 1 105 27 0.0 6 27 106 19 0.0 6 19 107 24 0.0 6 20 4 108 14 0.0 6 14 109 18 0.0 6 18 110 23 0.0 6 23 111 21 0.0 6 21 112 39 0.0 6 39 113 27 0.0 6 27 114 40 0.0 6 39 1 115 20 0.0 6 19 1 116 27 0.0 6 27 117 38 0.0 6 37 1 118 19 0.0 6 19 119 28 0.0 6 26 2 120 26 0.0 6 26 121 34 0.0 6 34 122 36 0.0 6 35 1 123 49 0.0 6 49 124 26 0.0 6 26 125 31 0.0 6 29 1 1 126 28 0.0 6 28 127 31 0.0 6 29 2 128 42 0.0 6 42 129 41 0.0 6 41 130 34 0.0 6 33 1 131 45 0.0 6 44 1 132 36 0.0 6 36 133 69 0.0 6 68 1 134 47 0.0 6 45 1 0 0 0 0 1 135 82 0.0 6 82 136 59 0.0 6 57 2 137 73 0.0 6 71 1 1 138 52 0.0 6 52 139 42 0.0 6 42 140 59 0.0 6 58 1 141 63 0.0 6 63 142 123 0.0 6 122 1 143 481 0.0 6 480 1 144 236 0.0 6 235 1 145 1146 0.0 6 1144 2 146 249 0.0 6 249 147 555 0.0 6 552 3 148 1197 0.0 6 1192 3 0 1 0 0 1 149 860 0.0 6 857 2 1 150 1101 0.0 6 1096 3 2 151 1284 0.0 6 1280 4 152 295 0.0 6 293 2 153 619 0.0 6 617 2 154 381 0.0 6 379 1 0 0 1 155 1011 0.0 6 1010 0 0 1 156 1998 0.0 6 1994 4 157 439 0.0 6 434 5 158 168 0.0 6 165 3 159 143 0.0 6 141 2 160 432 0.0 6 430 1 0 0 0 1 161 249 0.0 6 246 3 162 1156 0.0 6 1152 4 163 1377 0.0 6 1375 2 164 422 0.0 6 420 1 0 0 0 1 165 293 0.0 6 290 2 0 0 1 166 1727 0.0 6 1722 5 167 724 0.0 6 718 5 0 0 1 168 3158 0.0 6 3153 4 0 0 1 169 752 0.0 6 751 1 170 1676 0.0 6 1673 3 171 376 0.0 6 375 0 1 172 142 0.0 6 141 1 173 160 0.0 6 158 2 174 182 0.0 6 182 175 341 0.0 6 339 2 176 325 0.0 6 324 1 177 1359 0.0 6 1353 6 178 2336 0.0 6 2332 2 0 0 0 1 1 179 295 0.0 6 295 180 350 0.0 6 347 3 181 1985 0.0 6 1983 2 182 436 0.0 6 433 3 183 206 0.0 6 203 3 184 360 0.0 6 355 5 185 2711 0.0 6 2706 5 186 418 0.0 6 416 2 187 186 0.0 6 185 1 188 299 0.0 6 298 1 189 421 0.0 6 419 2 190 2251 0.0 6 2243 7 0 1 191 334 0.0 6 331 3 192 192 0.0 6 191 1 193 143 0.0 6 141 2 194 214 0.0 6 211 3 195 178 0.0 6 177 1 196 309 0.0 6 307 2 197 389 0.0 6 387 2 198 532 0.0 6 524 7 1 199 995 0.0 6 989 4 1 1 200 3399 0.0 6 3393 6 201 680 0.0 6 674 6 202 530 0.0 6 523 5 1 0 0 0 1 203 1029 0.0 6 1017 10 2 204 3351 0.0 6 3341 10 205 705 0.0 6 704 1 206 1769 0.0 6 1763 6 207 352 0.0 6 347 5 208 264 0.0 6 260 3 1 209 231 0.0 6 227 4 210 281 0.0 6 275 6 211 363 0.0 6 361 2 212 160 0.0 6 157 3 213 233 0.0 6 226 6 1 214 332 0.0 6 328 4 215 265 0.0 6 259 6 216 350 0.0 6 341 8 0 0 0 0 1 217 488 0.0 6 485 2 1 218 418 0.0 6 413 5 219 505 0.0 6 497 8 220 576 0.0 6 571 4 0 0 0 1 221 602 0.0 6 597 5 222 320 0.0 6 317 3 223 482 0.0 6 478 4 224 363 0.0 6 362 1 225 550 0.0 6 545 5 226 1143 0.0 6 1135 8 227 1798 0.0 6 1786 11 0 0 0 0 1 228 729 0.0 6 724 5 229 875 0.0 6 864 10 1 230 424 0.0 6 415 9 231 337 0.0 6 332 4 0 0 0 0 1 232 404 0.0 6 394 10 233 415 0.0 6 408 7 234 502 0.0 6 496 5 0 0 0 0 1 235 464 0.0 6 455 9 236 479 0.0 6 474 5 237 583 0.0 6 581 2 238 633 0.0 6 626 5 1 0 0 0 1 239 1078 0.0 6 1073 5 240 577 0.0 6 569 7 0 0 0 0 1 241 589 0.0 6 581 7 0 0 0 1 242 635 0.0 6 628 7 243 782 0.0 6 777 5 244 1655 0.0 6 1649 6 245 347 0.0 6 343 4 246 249 0.0 6 248 1 247 381 0.0 6 377 4 248 366 0.0 6 364 2 249 380 0.0 6 373 7 250 367 0.0 6 364 3 251 391 0.0 6 384 7 252 465 0.0 6 456 9 253 408 0.0 6 401 7 254 543 0.0 6 535 8 255 415 0.0 6 404 11 256 410 0.0 6 404 6 257 416 0.0 6 410 5 1 258 450 0.0 6 438 9 2 0 0 1 259 703 0.0 6 687 16 260 1295 0.0 6 1284 10 1 261 352 0.0 6 346 4 2 262 510 0.0 6 504 5 1 263 544 0.0 6 529 14 0 0 0 1 264 417 0.0 6 406 11 265 614 0.0 6 602 12 266 445 0.0 6 441 4 267 382 0.0 6 374 8 268 346 0.0 6 334 12 269 467 0.0 6 461 6 270 417 0.0 6 410 7 271 729 0.0 6 713 15 1 272 1275 0.0 6 1251 23 0 0 0 0 1 273 337 0.0 6 323 13 1 274 337 0.0 6 322 15 275 410 0.0 6 397 12 1 276 718 0.0 6 699 19 277 673 0.0 6 664 7 1 0 0 0 1 278 292 0.0 6 285 7 279 542 0.0 6 524 17 1 280 872 0.0 6 832 36 3 1 281 13669 0.0 6 13372 293 4 282 6 0.0 6 5 0 1 283 2 0.0 6 2 284 2 0.0 6 2 289 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 3120 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 20 442.1 1 20 60 1 0.0 6 0 1 67 1 0.0 6 0 1 68 1 0.0 6 1 69 4 0.0 6 4 70 17 0.0 6 17 71 22 0.0 6 22 72 42 0.0 6 41 1 73 6 0.0 6 6 74 25 0.0 6 25 75 43 0.0 6 43 76 35 0.0 6 35 77 44 0.0 6 44 78 50 0.0 6 50 79 14 0.0 6 14 80 24 0.0 6 24 81 15 0.0 6 15 82 47 0.0 6 47 83 75 0.0 6 74 1 84 4 0.0 6 4 86 2 0.0 6 2 87 18 0.0 6 18 88 12 0.0 6 12 89 51 0.0 6 51 90 38 0.0 6 37 1 91 4 0.0 6 4 92 20 0.0 6 20 93 76 0.0 6 76 94 55 0.0 6 54 1 95 135 0.0 6 135 96 31 0.0 6 31 97 53 0.0 6 53 101 2 0.0 6 2 102 8 0.0 6 8 103 17 0.0 6 17 104 59 0.0 6 59 105 84 0.0 6 84 106 4 0.0 6 4 107 37 0.0 6 37 108 77 0.0 6 77 109 1 0.0 6 1 110 1 0.0 6 1 111 35 0.0 6 35 112 93 0.0 6 93 113 6 0.0 6 5 1 114 2 0.0 6 2 115 7 0.0 6 7 116 29 0.0 6 29 117 98 0.0 6 98 118 7 0.0 6 7 122 1 0.0 6 1 124 1 0.0 6 1 125 1 0.0 6 1 126 45 0.0 6 45 127 111 0.0 6 111 128 5 0.0 6 5 129 2 0.0 6 1 1 130 52 0.0 6 52 131 154 0.0 6 152 2 132 27 0.0 6 27 133 71 0.0 6 71 134 4 0.0 6 4 141 3 0.0 6 3 142 1 0.0 6 1 144 1 0.0 6 1 145 3 0.0 6 3 146 1 0.0 6 1 149 1 0.0 6 1 150 1 0.0 6 1 151 6 0.0 6 6 152 1 0.0 6 1 168 1 0.0 6 1 169 1 0.0 6 1 170 22 0.0 6 22 171 54 0.0 6 54 172 1 0.0 6 1 179 1 0.0 6 1 184 5 0.0 6 5 185 2 0.0 6 2 186 54 0.0 6 54 187 179 0.0 6 177 2 188 51 0.0 6 51 189 74 0.0 6 74 190 5 0.0 6 4 1 194 1 0.0 6 1 197 2 0.0 6 2 198 28 0.0 6 27 1 199 117 0.0 6 117 200 16 0.0 6 16 201 2 0.0 6 2 202 5 0.0 6 5 203 33 0.0 6 33 204 89 0.0 6 88 0 0 0 0 0 1 205 4 0.0 6 4 206 1 0.0 6 1 207 10 0.0 6 10 208 16 0.0 6 16 209 1 0.0 6 1 210 1 0.0 6 0 0 0 0 0 0 1 212 4 0.0 6 4 213 15 0.0 6 15 214 1 0.0 6 1 217 1 0.0 6 1 219 18 0.0 6 18 220 66 0.0 6 66 221 1 0.0 6 1 227 1 0.0 6 1 230 5 0.0 6 5 231 47 0.0 6 47 232 103 0.0 6 103 233 7 0.0 6 7 234 1 0.0 6 1 237 1 0.0 6 1 266 1 0.0 6 1 267 6 0.0 6 6 268 16 0.0 6 16 269 3 0.0 6 3 299 1 0.0 6 1 300 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 109893 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 1768.2 0 0 3 6 1 27.6 1 1 7 1 6.9 2 0 1 9 1 0.4 2 1 10 3 0.1 3 2 1 11 5 0.0 3 5 12 1 0.0 3 0 1 13 9 0.0 3 8 1 14 7 0.0 4 4 3 15 1 0.0 4 1 16 25 0.0 4 23 2 17 23 0.0 5 18 4 1 18 20 0.0 5 18 2 19 18 0.0 5 16 2 20 116 0.0 6 111 5 21 99 0.0 6 96 3 22 49 0.0 6 47 2 23 33 0.0 6 32 1 24 33 0.0 6 31 2 25 36 0.0 6 34 1 1 26 34 0.0 6 32 2 27 23 0.0 6 23 28 31 0.0 6 28 2 0 0 0 0 1 29 29 0.0 6 26 1 1 0 0 0 1 30 27 0.0 6 26 1 31 17 0.0 6 15 2 32 17 0.0 6 16 1 33 10 0.0 6 7 3 34 17 0.0 6 16 1 35 14 0.0 6 14 36 7 0.0 6 3 2 1 0 0 0 1 37 13 0.0 6 13 38 12 0.0 6 12 39 19 0.0 6 18 1 40 15 0.0 6 14 1 41 17 0.0 6 15 1 0 1 42 12 0.0 6 12 43 24 0.0 6 21 2 1 44 14 0.0 6 14 45 14 0.0 6 12 1 0 1 46 21 0.0 6 19 2 47 19 0.0 6 17 2 48 24 0.0 6 21 3 49 23 0.0 6 20 2 0 0 0 0 1 50 20 0.0 6 19 1 51 16 0.0 6 15 1 52 30 0.0 6 24 5 0 0 0 1 53 16 0.0 6 15 1 54 31 0.0 6 27 4 55 18 0.0 6 16 2 56 20 0.0 6 18 2 57 27 0.0 6 23 4 58 27 0.0 6 25 2 59 25 0.0 6 23 1 1 60 23 0.0 6 21 2 61 19 0.0 6 19 62 27 0.0 6 25 1 0 0 0 1 63 30 0.0 6 26 4 64 40 0.0 6 36 2 1 1 65 30 0.0 6 26 3 0 0 0 0 1 66 41 0.0 6 36 3 1 0 0 1 67 212 0.0 6 197 11 3 1 68 60 0.0 6 58 2 69 90 0.0 6 87 3 70 33 0.0 6 31 2 71 63 0.0 6 58 4 0 1 72 50 0.0 6 47 2 1 73 25 0.0 6 23 1 0 0 0 0 1 74 24 0.0 6 22 2 75 27 0.0 6 25 2 76 39 0.0 6 35 4 77 41 0.0 6 35 3 0 0 2 0 1 78 30 0.0 6 29 1 79 39 0.0 6 38 1 80 46 0.0 6 44 2 81 31 0.0 6 28 3 82 43 0.0 6 41 1 0 0 0 1 83 86 0.0 6 83 3 84 41 0.0 6 34 4 3 85 35 0.0 6 34 1 86 44 0.0 6 40 4 87 49 0.0 6 45 4 88 29 0.0 6 27 2 89 49 0.0 6 47 2 90 55 0.0 6 49 6 91 60 0.0 6 56 4 92 55 0.0 6 54 1 93 81 0.0 6 77 3 1 94 77 0.0 6 75 1 1 95 80 0.0 6 75 5 96 80 0.0 6 78 1 1 97 64 0.0 6 62 1 1 98 63 0.0 6 59 4 99 61 0.0 6 57 3 0 0 0 1 100 80 0.0 6 75 5 101 99 0.0 6 95 4 102 98 0.0 6 96 2 103 102 0.0 6 98 4 104 128 0.0 6 123 5 105 98 0.0 6 96 2 106 74 0.0 6 70 3 1 107 99 0.0 6 93 5 1 108 95 0.0 6 91 4 109 91 0.0 6 86 5 110 140 0.0 6 135 3 2 111 134 0.0 6 130 3 0 0 1 112 138 0.0 6 134 3 0 0 1 113 87 0.0 6 82 5 114 132 0.0 6 125 6 1 115 120 0.0 6 116 4 116 121 0.0 6 118 3 117 147 0.0 6 144 1 1 0 0 1 118 125 0.0 6 122 3 119 132 0.0 6 131 0 0 1 120 174 0.0 6 169 5 121 177 0.0 6 172 5 122 156 0.0 6 148 6 1 0 0 0 1 123 193 0.0 6 187 3 2 0 1 124 179 0.0 6 175 4 125 232 0.0 6 225 7 126 223 0.0 6 216 4 3 127 228 0.0 6 224 3 1 128 209 0.0 6 205 2 1 0 1 129 275 0.0 6 272 3 130 255 0.0 6 250 5 131 328 0.0 6 325 3 132 334 0.0 6 325 8 0 0 0 0 1 133 376 0.0 6 370 6 134 514 0.0 6 509 5 135 516 0.0 6 504 11 0 0 0 1 136 430 0.0 6 417 9 1 1 0 1 1 137 444 0.0 6 436 8 138 711 0.0 6 705 6 139 616 0.0 6 605 11 140 1343 0.0 6 1331 12 141 820 0.0 6 812 7 1 142 1230 0.0 6 1214 14 0 0 1 1 143 1420 0.0 6 1409 10 1 144 1315 0.0 6 1306 8 0 0 0 0 1 145 1456 0.0 6 1443 12 1 146 1702 0.0 6 1690 11 0 0 0 1 147 799 0.0 6 789 8 1 0 0 1 148 1292 0.0 6 1279 10 1 0 1 0 1 149 1023 0.0 6 1005 16 0 0 0 1 1 150 2277 0.0 6 2253 18 2 1 1 1 1 151 2024 0.0 6 2003 11 0 4 2 4 152 1876 0.0 6 1825 43 0 0 0 7 1 153 1161 0.0 6 1139 19 0 0 0 2 1 154 1181 0.0 6 1153 21 2 1 0 2 2 155 1559 0.0 6 1539 14 3 0 1 2 156 1343 0.0 6 1329 13 1 157 2007 0.0 6 1986 19 0 1 0 0 1 158 1886 0.0 6 1866 16 1 1 1 0 1 159 1338 0.0 6 1330 8 160 1486 0.0 6 1466 18 2 161 1745 0.0 6 1730 13 1 0 0 0 1 162 1151 0.0 6 1134 16 1 163 2283 0.0 6 2260 21 1 0 0 1 164 1352 0.0 6 1332 19 0 0 0 0 1 165 1547 0.0 6 1529 15 2 1 166 1007 0.0 6 982 22 3 167 893 0.0 6 874 17 0 0 0 1 1 168 1389 0.0 6 1364 22 1 0 2 169 1239 0.0 6 1221 18 170 934 0.0 6 924 10 171 1358 0.0 6 1328 27 2 0 1 172 1680 0.0 6 1658 22 173 1718 0.0 6 1707 11 174 1313 0.0 6 1296 14 2 0 1 175 1426 0.0 6 1410 16 176 1831 0.0 6 1816 10 5 177 1088 0.0 6 1070 17 1 178 1447 0.0 6 1428 19 179 1110 0.0 6 1098 8 3 0 1 180 1253 0.0 6 1239 12 0 1 1 181 923 0.0 6 903 18 2 182 782 0.0 6 766 14 1 0 1 183 1317 0.0 6 1296 20 0 0 1 184 1683 0.0 6 1654 26 3 185 2155 0.0 6 2128 24 2 0 0 0 1 186 1248 0.0 6 1210 32 4 0 0 1 1 187 1100 0.0 6 1070 29 0 0 1 188 1430 0.0 6 1389 35 5 1 189 1245 0.0 6 1211 30 3 1 190 1397 0.0 6 1360 34 2 0 1 191 1492 0.0 6 1456 35 1 192 880 0.0 6 862 18 193 855 0.0 6 836 17 0 0 1 1 194 1017 0.0 6 994 23 195 1382 0.0 6 1342 39 1 196 2107 0.0 6 2043 59 0 0 5 197 1634 0.0 6 1593 36 4 0 0 1 198 1470 0.0 6 1439 28 1 0 1 0 1 199 2013 0.0 6 1976 36 0 0 1 200 932 0.0 6 911 19 2 201 975 0.0 6 957 17 0 0 1 202 797 0.0 6 784 12 0 0 1 203 625 0.0 6 616 9 204 703 0.0 6 689 14 205 472 0.0 6 470 2 206 399 0.0 6 394 4 0 0 1 207 382 0.0 6 377 4 0 0 1 208 451 0.0 6 442 9 209 360 0.0 6 356 4 210 300 0.0 6 292 7 1 211 304 0.0 6 300 4 212 343 0.0 6 336 6 1 213 508 0.0 6 505 2 0 0 1 214 600 0.0 6 588 10 0 1 1 215 414 0.0 6 408 6 216 513 0.0 6 501 11 0 0 0 1 217 389 0.0 6 385 3 1 218 333 0.0 6 332 1 219 269 0.0 6 265 4 220 113 0.0 6 109 3 0 0 0 1 221 350 0.0 6 343 6 1 222 410 0.0 6 403 7 223 436 0.0 6 421 14 0 0 1 224 115 0.0 6 114 1 225 220 0.0 6 219 1 226 122 0.0 6 122 227 172 0.0 6 166 5 0 0 0 1 228 99 0.0 6 97 2 229 168 0.0 6 164 4 230 107 0.0 6 101 5 0 0 1 231 145 0.0 6 140 5 232 201 0.0 6 194 7 233 102 0.0 6 101 1 234 63 0.0 6 60 3 235 37 0.0 6 37 236 17 0.0 6 17 237 17 0.0 6 17 238 19 0.0 6 19 239 14 0.0 6 14 240 49 0.0 6 47 2 241 74 0.0 6 72 1 0 0 1 242 63 0.0 6 61 2 243 63 0.0 6 61 2 244 43 0.0 6 42 1 245 12 0.0 6 12 246 11 0.0 6 11 247 6 0.0 6 5 1 248 9 0.0 6 8 1 249 2 0.0 6 2 250 9 0.0 6 9 251 11 0.0 6 10 1 252 2 0.0 6 2 253 1 0.0 6 1 274 1 0.0 6 1 276 1 0.0 6 1 277 1 0.0 6 1 278 24 0.0 6 23 1 279 34 0.0 6 32 0 1 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 3208 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 1 0.0 6 1 71 3 0.0 6 3 72 28 0.0 6 28 73 10 0.0 6 10 74 56 0.0 6 56 75 5 0.0 6 5 76 33 0.0 6 33 77 48 0.0 6 48 78 26 0.0 6 26 79 57 0.0 6 57 80 44 0.0 6 44 81 4 0.0 6 4 82 32 0.0 6 32 83 20 0.0 6 20 84 54 0.0 6 54 85 121 0.0 6 121 86 4 0.0 6 4 88 9 0.0 6 9 89 12 0.0 6 12 90 12 0.0 6 12 91 55 0.0 6 55 92 77 0.0 6 77 93 4 0.0 6 4 94 17 0.0 6 17 95 87 0.0 6 87 96 40 0.0 6 40 97 140 0.0 6 140 98 33 0.0 6 33 99 100 0.0 6 100 100 2 0.0 6 1 0 0 1 101 1 0.0 6 1 103 4 0.0 6 2 0 0 0 2 104 6 0.0 6 6 105 6 0.0 6 6 106 75 0.0 6 75 107 124 0.0 6 124 108 2 0.0 6 2 109 17 0.0 6 17 110 128 0.0 6 127 0 0 1 111 1 0.0 6 1 112 2 0.0 6 2 113 26 0.0 6 26 114 136 0.0 6 136 115 1 0.0 6 1 116 3 0.0 6 3 117 14 0.0 6 13 0 0 1 118 35 0.0 6 34 0 0 0 1 119 118 0.0 6 118 120 5 0.0 6 5 124 1 0.0 6 1 125 1 0.0 6 1 127 5 0.0 6 5 128 28 0.0 6 28 129 173 0.0 6 173 131 8 0.0 6 8 132 38 0.0 6 38 133 244 0.0 6 244 134 28 0.0 6 28 135 106 0.0 6 106 136 7 0.0 6 6 0 0 1 137 3 0.0 6 1 0 0 0 2 142 1 0.0 6 1 143 2 0.0 6 1 1 148 3 0.0 6 3 152 2 0.0 6 2 153 4 0.0 6 4 169 1 0.0 6 1 170 1 0.0 6 1 171 2 0.0 6 1 1 172 13 0.0 6 13 173 79 0.0 6 79 174 3 0.0 6 3 182 1 0.0 6 1 183 1 0.0 6 1 187 1 0.0 6 1 188 30 0.0 6 29 1 189 113 0.0 6 113 190 23 0.0 6 23 191 76 0.0 6 76 192 3 0.0 6 3 194 1 0.0 6 0 0 0 1 195 1 0.0 6 0 0 0 0 1 196 1 0.0 6 0 1 199 2 0.0 6 2 200 15 0.0 6 15 201 92 0.0 6 92 202 10 0.0 6 10 203 1 0.0 6 1 204 3 0.0 6 3 205 18 0.0 6 18 206 89 0.0 6 89 208 1 0.0 6 1 209 5 0.0 6 5 210 23 0.0 6 22 1 211 2 0.0 6 2 214 2 0.0 6 1 0 0 0 1 215 8 0.0 6 8 220 1 0.0 6 1 221 6 0.0 6 5 1 222 49 0.0 6 49 223 1 0.0 6 1 232 2 0.0 6 2 233 6 0.0 6 6 234 28 0.0 6 28 235 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_16_91_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_16_318_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_16_91_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_16_318_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.15 s (19 µs/read; 3.10 M reads/minute). === Summary === Total read pairs processed: 111,073 Read 1 with adapter: 111,073 (100.0%) Read 2 with adapter: 111,012 (99.9%) == Read fate breakdown == Pairs that were too short: 36 (0.0%) Pairs discarded as untrimmed: 61 (0.1%) Pairs written (passing filters): 110,976 (99.9%) Total basepairs processed: 46,244,491 bp Read 1: 24,894,715 bp Read 2: 21,349,776 bp Total written (filtered): 7,092,652 bp (15.3%) Read 1: 3,580,901 bp Read 2: 3,511,751 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 106866 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 3 27.1 1 2 0 1 7 2 6.8 2 1 1 9 7 0.4 2 4 3 10 2 0.1 3 2 11 1 0.0 3 1 14 1 0.0 4 1 15 2 0.0 4 2 16 10 0.0 4 9 1 17 3 0.0 5 2 1 18 2 0.0 5 1 1 19 6 0.0 5 5 1 20 254 0.0 6 245 9 21 109 0.0 6 102 7 22 150 0.0 6 146 3 1 23 93 0.0 6 86 5 1 1 24 86 0.0 6 83 2 1 25 74 0.0 6 73 1 26 55 0.0 6 52 1 1 1 27 33 0.0 6 29 3 1 28 25 0.0 6 24 1 29 20 0.0 6 20 30 14 0.0 6 14 31 6 0.0 6 6 32 9 0.0 6 8 0 1 33 12 0.0 6 10 1 1 34 7 0.0 6 7 35 10 0.0 6 9 1 36 6 0.0 6 6 37 16 0.0 6 16 38 8 0.0 6 8 39 6 0.0 6 5 1 40 5 0.0 6 5 41 18 0.0 6 18 42 8 0.0 6 7 1 43 8 0.0 6 7 1 44 17 0.0 6 16 1 45 5 0.0 6 5 46 7 0.0 6 7 47 8 0.0 6 7 1 48 12 0.0 6 12 49 6 0.0 6 5 1 50 3 0.0 6 2 1 51 19 0.0 6 18 1 52 17 0.0 6 17 53 12 0.0 6 12 54 9 0.0 6 9 55 12 0.0 6 12 56 3 0.0 6 2 1 57 8 0.0 6 7 1 58 4 0.0 6 4 59 6 0.0 6 5 1 60 7 0.0 6 7 61 7 0.0 6 7 62 7 0.0 6 6 1 63 12 0.0 6 12 64 10 0.0 6 9 0 1 65 6 0.0 6 6 66 9 0.0 6 9 67 5 0.0 6 5 68 2 0.0 6 2 69 7 0.0 6 6 0 0 0 0 1 70 8 0.0 6 8 71 10 0.0 6 10 72 13 0.0 6 13 73 13 0.0 6 13 74 14 0.0 6 13 1 75 15 0.0 6 15 76 13 0.0 6 12 0 0 1 77 6 0.0 6 6 78 6 0.0 6 6 79 14 0.0 6 13 1 80 6 0.0 6 6 81 14 0.0 6 14 82 9 0.0 6 8 0 0 0 1 83 4 0.0 6 4 84 12 0.0 6 12 85 16 0.0 6 14 2 86 18 0.0 6 18 87 21 0.0 6 20 1 88 12 0.0 6 11 1 89 20 0.0 6 20 90 20 0.0 6 20 91 15 0.0 6 15 92 12 0.0 6 10 2 93 5 0.0 6 5 94 18 0.0 6 18 95 10 0.0 6 10 96 10 0.0 6 9 1 97 16 0.0 6 15 1 98 15 0.0 6 14 1 99 19 0.0 6 19 100 21 0.0 6 21 101 22 0.0 6 22 102 16 0.0 6 16 103 28 0.0 6 25 3 104 28 0.0 6 28 105 34 0.0 6 33 0 1 106 33 0.0 6 32 1 107 40 0.0 6 40 108 36 0.0 6 36 109 38 0.0 6 38 110 34 0.0 6 34 111 22 0.0 6 22 112 40 0.0 6 40 113 34 0.0 6 33 1 114 28 0.0 6 28 115 22 0.0 6 21 1 116 36 0.0 6 35 0 0 0 1 117 47 0.0 6 47 118 38 0.0 6 38 119 18 0.0 6 17 1 120 37 0.0 6 35 2 121 28 0.0 6 28 122 34 0.0 6 34 123 41 0.0 6 41 124 36 0.0 6 36 125 33 0.0 6 33 126 58 0.0 6 58 127 57 0.0 6 57 128 43 0.0 6 41 2 129 28 0.0 6 28 130 43 0.0 6 43 131 57 0.0 6 57 132 61 0.0 6 60 1 133 62 0.0 6 62 134 67 0.0 6 65 2 135 49 0.0 6 49 136 70 0.0 6 68 2 137 65 0.0 6 65 138 97 0.0 6 97 139 95 0.0 6 95 140 145 0.0 6 145 141 160 0.0 6 159 1 142 933 0.0 6 932 1 143 1366 0.0 6 1366 144 363 0.0 6 361 2 145 1741 0.0 6 1736 3 1 0 1 146 1841 0.0 6 1835 4 0 0 0 2 147 811 0.0 6 811 148 264 0.0 6 263 1 149 278 0.0 6 276 1 0 0 1 150 1214 0.0 6 1209 4 0 0 0 0 1 151 3050 0.0 6 3044 4 1 0 0 1 152 609 0.0 6 606 3 153 1900 0.0 6 1896 3 0 0 1 154 391 0.0 6 391 155 579 0.0 6 578 1 156 228 0.0 6 227 1 157 173 0.0 6 173 158 331 0.0 6 327 4 159 682 0.0 6 679 3 160 1884 0.0 6 1878 6 161 364 0.0 6 363 1 162 273 0.0 6 273 163 142 0.0 6 142 164 200 0.0 6 199 1 165 327 0.0 6 326 1 166 2606 0.0 6 2603 3 167 826 0.0 6 824 2 168 213 0.0 6 213 169 280 0.0 6 276 4 170 482 0.0 6 480 2 171 3059 0.0 6 3053 5 0 0 0 1 172 640 0.0 6 640 173 2843 0.0 6 2838 5 174 796 0.0 6 793 1 1 0 0 0 1 175 251 0.0 6 251 176 961 0.0 6 958 3 177 455 0.0 6 453 2 178 213 0.0 6 212 1 179 148 0.0 6 147 1 180 196 0.0 6 195 1 181 243 0.0 6 239 3 0 0 0 1 182 284 0.0 6 283 1 183 321 0.0 6 319 1 0 0 0 1 184 658 0.0 6 655 3 185 257 0.0 6 253 3 0 0 0 0 1 186 314 0.0 6 314 187 371 0.0 6 369 2 188 412 0.0 6 410 2 189 465 0.0 6 462 3 190 695 0.0 6 693 2 191 3680 0.0 6 3671 9 192 1489 0.0 6 1485 4 193 4721 0.0 6 4711 8 1 0 1 194 735 0.0 6 731 4 195 363 0.0 6 359 4 196 432 0.0 6 429 2 0 0 0 0 1 197 310 0.0 6 305 5 198 567 0.0 6 560 6 0 1 199 647 0.0 6 644 2 0 0 0 0 1 200 494 0.0 6 489 5 201 527 0.0 6 523 4 202 492 0.0 6 485 7 203 596 0.0 6 593 3 204 722 0.0 6 715 6 0 0 1 205 943 0.0 6 940 2 1 206 1104 0.0 6 1096 7 1 207 1750 0.0 6 1742 8 208 1782 0.0 6 1771 9 1 1 209 2383 0.0 6 2367 14 1 1 210 5769 0.0 6 5758 8 2 0 1 211 1323 0.0 6 1318 5 212 720 0.0 6 716 3 0 0 0 1 213 1021 0.0 6 1018 2 0 0 0 0 1 214 1054 0.0 6 1054 215 1313 0.0 6 1306 6 0 0 1 216 2062 0.0 6 2052 6 0 2 0 2 217 5599 0.0 6 5587 11 1 218 849 0.0 6 842 6 0 0 1 219 1124 0.0 6 1119 5 220 1171 0.0 6 1167 3 0 1 221 627 0.0 6 620 7 222 556 0.0 6 550 5 0 0 0 0 1 223 478 0.0 6 474 3 1 224 773 0.0 6 765 4 1 1 0 1 1 225 1419 0.0 6 1414 4 0 0 0 0 1 226 412 0.0 6 407 4 0 1 227 271 0.0 6 269 2 228 282 0.0 6 276 4 1 0 0 1 229 266 0.0 6 262 4 230 297 0.0 6 296 0 0 1 231 359 0.0 6 354 5 232 423 0.0 6 417 5 0 1 233 264 0.0 6 259 3 0 0 2 234 299 0.0 6 294 4 0 0 0 0 1 235 333 0.0 6 331 2 236 360 0.0 6 352 8 237 307 0.0 6 303 2 0 0 1 1 238 407 0.0 6 405 1 0 0 1 239 654 0.0 6 645 9 240 224 0.0 6 222 2 241 327 0.0 6 321 6 242 233 0.0 6 229 4 243 240 0.0 6 229 9 1 0 0 0 1 244 208 0.0 6 202 6 245 296 0.0 6 294 2 246 431 0.0 6 429 2 247 291 0.0 6 287 2 0 2 248 226 0.0 6 226 249 158 0.0 6 154 4 250 248 0.0 6 244 3 0 0 0 1 251 149 0.0 6 147 1 0 0 0 1 252 144 0.0 6 142 0 0 1 0 0 1 253 146 0.0 6 143 2 0 0 0 0 1 254 146 0.0 6 142 3 0 0 0 0 1 255 171 0.0 6 165 5 0 0 0 1 256 214 0.0 6 207 3 2 0 1 1 257 181 0.0 6 176 4 0 0 0 1 258 159 0.0 6 155 3 0 0 0 1 259 220 0.0 6 217 3 260 169 0.0 6 165 2 1 0 0 1 261 187 0.0 6 186 1 262 251 0.0 6 250 0 0 0 0 1 263 96 0.0 6 92 4 264 145 0.0 6 145 265 117 0.0 6 115 1 1 266 198 0.0 6 194 3 0 0 0 1 267 152 0.0 6 151 0 1 268 167 0.0 6 165 1 0 0 0 1 269 175 0.0 6 175 270 223 0.0 6 216 6 0 0 1 271 413 0.0 6 405 7 0 0 1 272 227 0.0 6 224 3 273 184 0.0 6 183 1 274 89 0.0 6 86 2 0 0 0 1 275 270 0.0 6 268 2 276 403 0.0 6 403 277 360 0.0 6 355 5 278 425 0.0 6 421 2 2 279 355 0.0 6 352 2 0 0 0 1 280 1333 0.0 6 1324 8 0 0 0 1 281 395 0.0 6 391 3 0 0 0 1 282 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 4207 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 17 433.9 1 17 70 15 0.0 6 15 71 49 0.0 6 48 1 72 50 0.0 6 50 73 23 0.0 6 22 1 74 88 0.0 6 88 75 72 0.0 6 72 76 30 0.0 6 30 77 2 0.0 6 2 78 21 0.0 6 21 79 81 0.0 6 81 80 140 0.0 6 140 81 32 0.0 6 32 82 68 0.0 6 67 1 83 13 0.0 6 13 84 15 0.0 6 15 85 5 0.0 6 5 86 2 0.0 6 1 0 0 0 0 0 1 87 7 0.0 6 7 88 38 0.0 6 38 89 75 0.0 6 75 90 1 0.0 6 1 91 6 0.0 6 6 92 2 0.0 6 2 93 3 0.0 6 3 94 41 0.0 6 41 95 116 0.0 6 116 96 18 0.0 6 18 97 1 0.0 6 1 98 3 0.0 6 3 99 41 0.0 6 41 100 137 0.0 6 135 2 101 42 0.0 6 42 102 125 0.0 6 125 103 12 0.0 6 12 104 13 0.0 6 13 105 43 0.0 6 43 106 5 0.0 6 5 107 2 0.0 6 2 111 8 0.0 6 8 113 3 0.0 6 3 116 1 0.0 6 1 117 1 0.0 6 1 118 10 0.0 6 10 119 52 0.0 6 52 120 163 0.0 6 163 121 74 0.0 6 74 122 228 0.0 6 227 1 123 7 0.0 6 7 124 6 0.0 6 6 127 1 0.0 6 1 131 2 0.0 6 2 132 4 0.0 6 4 134 5 0.0 6 5 135 22 0.0 6 22 136 37 0.0 6 37 137 12 0.0 6 11 1 138 120 0.0 6 120 139 324 0.0 6 323 1 140 57 0.0 6 57 142 1 0.0 6 1 143 8 0.0 6 8 144 14 0.0 6 13 1 145 144 0.0 6 144 146 374 0.0 6 374 147 32 0.0 6 32 148 47 0.0 6 47 149 83 0.0 6 83 150 6 0.0 6 6 151 18 0.0 6 18 152 5 0.0 6 4 0 0 0 0 0 1 153 59 0.0 6 59 154 171 0.0 6 170 0 0 0 0 0 1 155 21 0.0 6 21 159 2 0.0 6 1 0 0 0 0 0 1 160 10 0.0 6 10 161 25 0.0 6 25 164 5 0.0 6 5 165 18 0.0 6 18 166 1 0.0 6 1 167 6 0.0 6 6 168 30 0.0 6 30 169 6 0.0 6 6 170 34 0.0 6 34 171 2 0.0 6 2 172 2 0.0 6 2 174 4 0.0 6 4 175 26 0.0 6 26 176 8 0.0 6 8 177 34 0.0 6 34 178 13 0.0 6 13 179 36 0.0 6 36 180 1 0.0 6 1 182 4 0.0 6 4 183 7 0.0 6 7 190 1 0.0 6 1 191 3 0.0 6 3 192 3 0.0 6 3 193 1 0.0 6 1 194 3 0.0 6 3 195 3 0.0 6 3 199 1 0.0 6 1 200 16 0.0 6 16 201 48 0.0 6 48 202 1 0.0 6 1 203 1 0.0 6 1 204 2 0.0 6 2 205 5 0.0 6 5 206 1 0.0 6 1 208 5 0.0 6 5 209 18 0.0 6 18 219 1 0.0 6 1 220 1 0.0 6 1 229 1 0.0 6 1 231 22 0.0 6 22 232 45 0.0 6 44 1 234 1 0.0 6 0 0 1 245 5 0.0 6 5 246 21 0.0 6 21 247 54 0.0 6 54 248 3 0.0 6 3 254 3 0.0 6 3 259 2 0.0 6 2 277 1 0.0 6 1 280 16 0.0 6 16 281 24 0.0 6 24 282 1 0.0 6 1 283 2 0.0 6 2 284 4 0.0 6 4 288 1 0.0 6 1 289 2 0.0 6 2 290 4 0.0 6 4 297 1 0.0 6 1 298 1 0.0 6 1 304 9 0.0 6 9 305 20 0.0 6 20 306 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 106650 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 108.5 1 1 6 1 27.1 1 1 9 2 0.4 2 2 10 1 0.1 3 1 11 4 0.0 3 3 1 12 9 0.0 3 9 13 20 0.0 3 19 1 14 5 0.0 4 4 0 0 1 16 25 0.0 4 24 1 17 25 0.0 5 22 2 1 18 15 0.0 5 13 2 19 20 0.0 5 18 1 1 20 146 0.0 6 140 6 21 104 0.0 6 94 9 1 22 72 0.0 6 69 2 1 23 58 0.0 6 54 3 1 24 20 0.0 6 19 1 25 20 0.0 6 18 2 26 25 0.0 6 22 2 1 27 26 0.0 6 25 1 28 11 0.0 6 9 2 29 14 0.0 6 12 2 30 11 0.0 6 10 1 31 15 0.0 6 14 1 32 19 0.0 6 17 1 1 33 14 0.0 6 13 1 34 7 0.0 6 7 35 13 0.0 6 12 1 36 18 0.0 6 17 1 37 12 0.0 6 10 2 38 10 0.0 6 10 39 13 0.0 6 12 1 40 7 0.0 6 7 41 12 0.0 6 12 42 16 0.0 6 16 43 9 0.0 6 8 1 44 13 0.0 6 12 1 45 12 0.0 6 12 46 18 0.0 6 18 47 22 0.0 6 20 2 48 15 0.0 6 14 1 49 13 0.0 6 12 1 50 26 0.0 6 25 1 51 23 0.0 6 21 2 52 21 0.0 6 20 1 53 37 0.0 6 34 2 0 0 0 0 1 54 27 0.0 6 25 2 55 29 0.0 6 28 1 56 34 0.0 6 31 3 57 25 0.0 6 22 2 0 1 58 32 0.0 6 29 3 59 23 0.0 6 22 1 60 31 0.0 6 27 3 0 0 0 0 1 61 18 0.0 6 13 2 3 62 39 0.0 6 35 3 1 63 20 0.0 6 16 3 0 1 64 27 0.0 6 26 1 65 54 0.0 6 53 0 0 0 0 0 1 66 38 0.0 6 37 1 67 42 0.0 6 41 0 0 0 0 1 68 52 0.0 6 50 2 69 198 0.0 6 185 11 1 1 70 74 0.0 6 68 5 0 0 0 0 1 71 113 0.0 6 103 10 72 36 0.0 6 35 1 73 67 0.0 6 65 2 74 35 0.0 6 33 1 1 75 28 0.0 6 27 0 1 76 36 0.0 6 35 1 77 46 0.0 6 45 1 78 43 0.0 6 42 0 1 79 45 0.0 6 45 80 44 0.0 6 40 3 1 81 57 0.0 6 55 2 82 53 0.0 6 51 2 83 74 0.0 6 71 3 84 63 0.0 6 61 0 1 0 0 0 1 85 89 0.0 6 89 86 41 0.0 6 39 2 87 50 0.0 6 45 5 88 47 0.0 6 46 1 89 60 0.0 6 58 1 0 0 0 0 1 90 47 0.0 6 45 1 1 91 47 0.0 6 44 2 1 92 66 0.0 6 64 2 93 69 0.0 6 68 1 94 76 0.0 6 74 2 95 80 0.0 6 76 4 96 75 0.0 6 70 2 1 0 1 0 1 97 75 0.0 6 69 2 3 1 98 105 0.0 6 102 3 99 88 0.0 6 83 4 0 1 100 90 0.0 6 89 0 0 0 0 1 101 58 0.0 6 57 1 102 75 0.0 6 74 0 0 1 103 85 0.0 6 82 2 1 104 80 0.0 6 72 6 0 2 105 117 0.0 6 113 4 106 200 0.0 6 193 5 2 107 128 0.0 6 125 2 0 1 108 114 0.0 6 109 5 109 130 0.0 6 125 5 110 125 0.0 6 125 111 163 0.0 6 155 6 1 0 1 112 147 0.0 6 141 4 2 113 205 0.0 6 200 5 114 212 0.0 6 210 2 115 126 0.0 6 120 5 0 0 1 116 214 0.0 6 214 117 194 0.0 6 187 5 2 118 159 0.0 6 156 2 1 119 239 0.0 6 234 5 120 202 0.0 6 198 3 1 121 219 0.0 6 213 5 0 0 0 0 1 122 228 0.0 6 221 7 123 173 0.0 6 172 1 124 188 0.0 6 183 4 1 125 238 0.0 6 235 2 1 126 263 0.0 6 261 2 127 276 0.0 6 273 3 128 342 0.0 6 334 6 0 0 0 1 1 129 294 0.0 6 288 6 130 364 0.0 6 358 5 0 1 131 434 0.0 6 427 6 0 0 0 1 132 370 0.0 6 361 8 0 0 0 0 1 133 431 0.0 6 424 7 134 490 0.0 6 480 8 1 0 0 0 1 135 572 0.0 6 567 5 136 689 0.0 6 680 9 137 740 0.0 6 733 6 0 0 0 0 1 138 744 0.0 6 735 9 139 1379 0.0 6 1372 7 140 1464 0.0 6 1457 5 1 1 141 839 0.0 6 833 5 0 0 0 0 1 142 1887 0.0 6 1875 11 0 0 0 0 1 143 1862 0.0 6 1857 2 1 1 0 1 144 1262 0.0 6 1255 7 145 808 0.0 6 806 1 1 146 1350 0.0 6 1338 10 0 0 2 147 1742 0.0 6 1727 13 1 0 0 1 148 3237 0.0 6 3223 12 1 0 0 1 149 1274 0.0 6 1259 12 2 0 0 0 1 150 2355 0.0 6 2337 16 0 0 0 2 151 1214 0.0 6 1193 17 2 1 1 152 1886 0.0 6 1868 15 2 1 153 1453 0.0 6 1440 11 1 0 0 1 154 1412 0.0 6 1397 15 155 1791 0.0 6 1771 15 3 0 0 1 1 156 1817 0.0 6 1798 16 2 1 157 2130 0.0 6 2112 16 1 0 0 1 158 1312 0.0 6 1296 10 3 0 0 1 2 159 1441 0.0 6 1435 4 0 1 0 1 160 1741 0.0 6 1732 8 0 1 161 1321 0.0 6 1312 8 0 0 1 162 1645 0.0 6 1634 10 0 1 163 2714 0.0 6 2704 9 1 164 1230 0.0 6 1217 9 0 1 1 1 1 165 1319 0.0 6 1308 10 1 166 1474 0.0 6 1458 14 1 0 0 1 167 1328 0.0 6 1321 6 0 1 168 2599 0.0 6 2588 7 1 2 0 1 169 1459 0.0 6 1449 9 1 170 2449 0.0 6 2433 12 1 1 1 1 171 1535 0.0 6 1530 4 0 0 1 172 1000 0.0 6 992 7 0 1 173 1818 0.0 6 1809 6 0 1 1 0 1 174 1301 0.0 6 1285 13 0 1 1 1 175 1073 0.0 6 1063 10 176 990 0.0 6 976 14 177 1050 0.0 6 1036 10 1 1 2 178 1104 0.0 6 1094 10 179 750 0.0 6 744 6 180 1234 0.0 6 1229 4 0 0 0 0 1 181 898 0.0 6 890 8 182 1129 0.0 6 1125 3 0 0 0 0 1 183 1055 0.0 6 1048 5 2 184 1166 0.0 6 1158 8 185 1408 0.0 6 1397 7 1 0 3 186 1660 0.0 6 1652 7 0 1 187 2090 0.0 6 2082 8 188 2168 0.0 6 2162 4 1 1 189 1109 0.0 6 1102 6 0 1 190 1510 0.0 6 1503 6 0 0 1 191 954 0.0 6 947 4 1 0 2 192 821 0.0 6 819 2 193 667 0.0 6 660 6 0 0 1 194 537 0.0 6 531 4 1 0 0 0 1 195 548 0.0 6 544 4 196 593 0.0 6 587 6 197 619 0.0 6 609 9 1 198 569 0.0 6 564 4 0 1 199 421 0.0 6 414 6 0 0 0 1 200 515 0.0 6 508 6 0 0 1 201 580 0.0 6 577 3 202 446 0.0 6 442 4 203 480 0.0 6 478 1 1 204 395 0.0 6 393 2 205 489 0.0 6 484 4 0 1 206 500 0.0 6 496 2 0 2 207 593 0.0 6 592 0 0 1 208 178 0.0 6 177 1 209 146 0.0 6 145 0 0 1 210 154 0.0 6 152 2 211 128 0.0 6 125 2 0 1 212 137 0.0 6 134 3 213 146 0.0 6 146 214 248 0.0 6 248 215 273 0.0 6 271 0 0 2 216 143 0.0 6 140 2 0 1 217 79 0.0 6 78 1 218 105 0.0 6 103 2 219 111 0.0 6 110 0 0 1 220 98 0.0 6 97 1 221 105 0.0 6 99 5 0 1 222 133 0.0 6 126 7 223 106 0.0 6 101 3 0 2 224 129 0.0 6 122 5 0 2 225 38 0.0 6 36 0 2 226 90 0.0 6 87 2 0 0 1 227 210 0.0 6 200 9 0 1 228 81 0.0 6 77 3 0 1 229 175 0.0 6 167 6 0 2 230 160 0.0 6 155 4 0 1 231 135 0.0 6 130 3 0 2 232 60 0.0 6 59 1 233 52 0.0 6 50 1 0 1 234 36 0.0 6 35 1 235 40 0.0 6 39 1 236 8 0.0 6 8 237 21 0.0 6 19 2 238 33 0.0 6 33 239 14 0.0 6 14 240 64 0.0 6 62 1 0 1 241 13 0.0 6 13 242 5 0.0 6 4 1 243 15 0.0 6 14 1 244 28 0.0 6 27 0 0 1 245 12 0.0 6 11 0 1 246 88 0.0 6 85 2 0 1 247 19 0.0 6 18 1 248 20 0.0 6 19 1 249 50 0.0 6 49 1 250 16 0.0 6 15 1 251 16 0.0 6 16 252 6 0.0 6 6 253 3 0.0 6 2 1 254 8 0.0 6 8 255 6 0.0 6 6 256 8 0.0 6 8 257 1 0.0 6 1 258 1 0.0 6 1 259 1 0.0 6 1 260 1 0.0 6 1 262 3 0.0 6 3 280 11 0.0 6 11 281 78 0.0 6 74 2 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 4362 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 2 0.0 6 2 70 8 0.0 6 8 71 48 0.0 6 48 72 63 0.0 6 63 73 13 0.0 6 12 1 74 97 0.0 6 97 75 89 0.0 6 89 76 32 0.0 6 31 1 78 13 0.0 6 11 0 0 0 2 79 91 0.0 6 91 80 164 0.0 6 163 0 0 0 1 81 31 0.0 6 31 82 107 0.0 6 107 83 9 0.0 6 9 84 24 0.0 6 23 0 0 0 1 85 4 0.0 6 2 0 0 0 2 86 2 0.0 6 2 87 13 0.0 6 13 88 32 0.0 6 32 89 96 0.0 6 96 90 2 0.0 6 2 91 8 0.0 6 7 0 0 0 1 92 2 0.0 6 2 93 3 0.0 6 3 94 34 0.0 6 34 95 134 0.0 6 134 96 25 0.0 6 25 97 4 0.0 6 4 98 2 0.0 6 1 0 0 0 1 99 29 0.0 6 27 0 0 0 2 100 173 0.0 6 172 1 101 37 0.0 6 37 102 156 0.0 6 156 103 34 0.0 6 34 104 8 0.0 6 7 0 0 0 1 105 55 0.0 6 55 106 9 0.0 6 8 0 0 0 1 108 1 0.0 6 1 109 2 0.0 6 2 111 6 0.0 6 6 113 3 0.0 6 3 115 1 0.0 6 1 118 4 0.0 6 4 119 42 0.0 6 41 1 120 215 0.0 6 215 121 48 0.0 6 48 122 239 0.0 6 237 1 0 1 123 13 0.0 6 12 0 0 1 124 8 0.0 6 6 0 0 0 2 125 1 0.0 6 1 126 2 0.0 6 0 0 0 0 2 129 1 0.0 6 1 131 2 0.0 6 2 132 5 0.0 6 5 134 6 0.0 6 6 135 24 0.0 6 24 136 42 0.0 6 42 137 6 0.0 6 5 1 138 106 0.0 6 106 139 470 0.0 6 468 1 0 1 140 72 0.0 6 71 1 142 4 0.0 6 4 143 5 0.0 6 3 0 0 0 2 144 8 0.0 6 7 0 0 0 1 145 99 0.0 6 99 146 460 0.0 6 460 147 19 0.0 6 19 148 52 0.0 6 52 149 73 0.0 6 71 0 0 1 1 150 10 0.0 6 8 1 0 0 1 151 20 0.0 6 20 152 6 0.0 6 5 0 0 1 153 52 0.0 6 51 1 154 223 0.0 6 223 155 35 0.0 6 34 0 0 0 1 156 1 0.0 6 0 0 0 1 157 1 0.0 6 0 0 0 1 160 3 0.0 6 3 161 27 0.0 6 27 164 7 0.0 6 6 0 0 1 165 17 0.0 6 17 166 1 0.0 6 1 167 3 0.0 6 3 168 21 0.0 6 21 169 5 0.0 6 5 170 30 0.0 6 30 172 1 0.0 6 0 0 0 0 1 173 2 0.0 6 2 174 7 0.0 6 7 175 40 0.0 6 40 176 6 0.0 6 6 177 33 0.0 6 33 178 8 0.0 6 8 179 27 0.0 6 26 1 181 1 0.0 6 1 183 7 0.0 6 7 190 2 0.0 6 2 191 1 0.0 6 1 192 6 0.0 6 6 193 1 0.0 6 1 194 1 0.0 6 1 199 3 0.0 6 3 200 12 0.0 6 12 201 36 0.0 6 36 202 1 0.0 6 1 204 1 0.0 6 1 209 3 0.0 6 3 219 1 0.0 6 1 231 3 0.0 6 3 232 4 0.0 6 4 233 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_17_92_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_17_319_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_17_92_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_17_319_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.32 s (28 µs/read; 2.11 M reads/minute). === Summary === Total read pairs processed: 117,012 Read 1 with adapter: 117,012 (100.0%) Read 2 with adapter: 116,940 (99.9%) == Read fate breakdown == Pairs that were too short: 1 (0.0%) Pairs discarded as untrimmed: 72 (0.1%) Pairs written (passing filters): 116,939 (99.9%) Total basepairs processed: 58,615,048 bp Read 1: 33,448,680 bp Read 2: 25,166,368 bp Total written (filtered): 6,907,675 bp (11.8%) Read 1: 3,512,602 bp Read 2: 3,395,073 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 116914 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 457.1 1 1 5 2 114.3 1 2 6 2 28.6 1 0 0 2 7 1 7.1 2 1 9 3 0.4 2 2 1 13 1 0.0 3 1 19 1 0.0 5 1 20 30 0.0 6 27 3 21 16 0.0 6 15 1 22 5 0.0 6 5 23 9 0.0 6 8 1 24 5 0.0 6 5 25 13 0.0 6 12 1 26 9 0.0 6 9 27 2 0.0 6 2 28 6 0.0 6 5 1 29 3 0.0 6 3 30 4 0.0 6 4 31 3 0.0 6 3 32 4 0.0 6 3 1 33 4 0.0 6 4 34 6 0.0 6 4 2 35 4 0.0 6 4 36 1 0.0 6 0 1 37 6 0.0 6 6 38 6 0.0 6 5 1 39 2 0.0 6 2 40 7 0.0 6 5 2 41 7 0.0 6 5 2 42 2 0.0 6 2 43 4 0.0 6 4 44 4 0.0 6 2 2 45 7 0.0 6 6 1 46 6 0.0 6 5 1 49 3 0.0 6 3 50 2 0.0 6 2 51 1 0.0 6 1 52 3 0.0 6 2 1 53 8 0.0 6 8 54 13 0.0 6 11 2 56 9 0.0 6 9 57 7 0.0 6 7 58 10 0.0 6 10 59 3 0.0 6 3 60 3 0.0 6 2 1 62 5 0.0 6 5 63 2 0.0 6 2 64 2 0.0 6 2 65 4 0.0 6 2 1 1 66 3 0.0 6 2 1 67 1 0.0 6 1 68 1 0.0 6 1 69 4 0.0 6 4 72 3 0.0 6 3 73 1 0.0 6 1 74 3 0.0 6 3 75 3 0.0 6 3 76 1 0.0 6 1 77 4 0.0 6 4 78 10 0.0 6 10 79 3 0.0 6 2 0 1 80 7 0.0 6 7 81 3 0.0 6 3 83 5 0.0 6 4 1 84 3 0.0 6 3 85 2 0.0 6 1 1 86 11 0.0 6 11 87 11 0.0 6 10 1 88 5 0.0 6 5 89 8 0.0 6 8 90 7 0.0 6 6 1 91 4 0.0 6 4 92 10 0.0 6 10 93 2 0.0 6 2 94 3 0.0 6 3 96 6 0.0 6 6 97 9 0.0 6 8 1 98 10 0.0 6 9 1 99 5 0.0 6 5 100 8 0.0 6 6 2 101 17 0.0 6 17 102 18 0.0 6 17 1 103 10 0.0 6 9 1 104 10 0.0 6 9 1 105 6 0.0 6 5 1 106 8 0.0 6 8 107 15 0.0 6 14 1 108 16 0.0 6 14 2 109 12 0.0 6 9 3 110 6 0.0 6 6 111 11 0.0 6 10 1 112 11 0.0 6 11 113 9 0.0 6 9 114 16 0.0 6 16 115 13 0.0 6 13 116 11 0.0 6 10 1 117 16 0.0 6 12 4 118 4 0.0 6 3 1 119 8 0.0 6 8 120 1 0.0 6 1 121 7 0.0 6 5 2 122 28 0.0 6 25 2 0 1 123 24 0.0 6 23 1 124 11 0.0 6 10 1 125 8 0.0 6 7 1 126 17 0.0 6 15 2 127 10 0.0 6 10 128 35 0.0 6 33 2 129 14 0.0 6 14 130 15 0.0 6 14 1 131 8 0.0 6 6 2 132 11 0.0 6 11 133 37 0.0 6 36 1 134 43 0.0 6 42 1 135 61 0.0 6 58 3 136 22 0.0 6 22 137 73 0.0 6 72 0 1 138 51 0.0 6 49 2 139 58 0.0 6 54 4 140 206 0.0 6 203 3 141 271 0.0 6 270 1 142 139 0.0 6 138 1 143 108 0.0 6 107 1 144 99 0.0 6 98 1 145 46 0.0 6 41 4 1 146 27 0.0 6 26 1 147 37 0.0 6 33 4 148 26 0.0 6 23 2 1 149 29 0.0 6 26 3 150 23 0.0 6 17 4 0 1 0 1 151 26 0.0 6 25 1 152 15 0.0 6 15 153 26 0.0 6 23 3 154 44 0.0 6 44 155 48 0.0 6 46 2 156 146 0.0 6 144 2 157 58 0.0 6 56 2 158 39 0.0 6 37 1 1 159 27 0.0 6 25 2 160 41 0.0 6 40 1 161 176 0.0 6 176 162 33 0.0 6 30 3 163 32 0.0 6 32 164 58 0.0 6 55 3 165 61 0.0 6 59 2 166 28 0.0 6 27 1 167 44 0.0 6 44 168 205 0.0 6 201 3 1 169 112 0.0 6 110 2 170 119 0.0 6 115 4 171 51 0.0 6 47 4 172 154 0.0 6 144 10 173 114 0.0 6 111 3 174 99 0.0 6 92 7 175 70 0.0 6 69 1 176 68 0.0 6 65 3 177 87 0.0 6 85 2 178 70 0.0 6 64 5 1 179 73 0.0 6 69 4 180 97 0.0 6 93 4 181 166 0.0 6 162 4 182 131 0.0 6 125 6 183 50 0.0 6 45 4 1 184 78 0.0 6 77 1 185 250 0.0 6 242 7 1 186 262 0.0 6 256 6 187 170 0.0 6 165 5 188 286 0.0 6 278 8 189 126 0.0 6 120 6 190 259 0.0 6 251 8 191 322 0.0 6 312 10 192 260 0.0 6 248 12 193 385 0.0 6 372 12 1 194 300 0.0 6 285 15 195 385 0.0 6 372 13 196 383 0.0 6 367 16 197 206 0.0 6 200 5 1 198 457 0.0 6 447 9 0 1 199 420 0.0 6 404 13 2 1 200 660 0.0 6 638 22 201 423 0.0 6 407 16 202 572 0.0 6 557 15 203 365 0.0 6 355 10 204 475 0.0 6 460 15 205 540 0.0 6 513 27 206 656 0.0 6 640 16 207 426 0.0 6 409 17 208 459 0.0 6 442 16 1 209 262 0.0 6 254 8 210 296 0.0 6 288 7 0 0 1 211 335 0.0 6 328 7 212 214 0.0 6 208 6 213 349 0.0 6 326 21 2 214 1051 0.0 6 1036 15 215 278 0.0 6 267 10 1 216 598 0.0 6 588 10 217 324 0.0 6 314 10 218 412 0.0 6 396 14 1 1 219 553 0.0 6 538 14 0 1 220 349 0.0 6 336 13 221 328 0.0 6 318 8 2 222 440 0.0 6 421 19 223 987 0.0 6 965 22 224 834 0.0 6 809 25 225 745 0.0 6 728 17 226 533 0.0 6 514 19 227 674 0.0 6 666 7 1 228 431 0.0 6 423 8 229 378 0.0 6 370 8 230 291 0.0 6 286 5 231 311 0.0 6 302 9 232 246 0.0 6 242 4 233 194 0.0 6 187 6 1 234 295 0.0 6 286 8 0 1 235 208 0.0 6 200 7 1 236 281 0.0 6 269 8 4 237 701 0.0 6 679 21 1 238 1217 0.0 6 1184 32 0 1 239 792 0.0 6 770 21 1 240 1119 0.0 6 1080 36 3 241 523 0.0 6 495 28 242 1172 0.0 6 1147 24 1 243 334 0.0 6 320 12 0 1 1 244 510 0.0 6 499 11 245 419 0.0 6 402 17 246 937 0.0 6 917 20 247 482 0.0 6 460 22 248 706 0.0 6 680 24 2 249 612 0.0 6 592 20 250 665 0.0 6 634 30 1 251 1128 0.0 6 1090 38 252 825 0.0 6 805 18 2 253 546 0.0 6 526 20 254 484 0.0 6 458 25 1 255 639 0.0 6 601 33 4 0 0 0 1 256 880 0.0 6 852 27 1 257 1230 0.0 6 1206 24 258 491 0.0 6 471 19 0 1 259 966 0.0 6 922 44 260 1411 0.0 6 1375 35 1 261 935 0.0 6 902 32 1 262 608 0.0 6 587 20 1 263 476 0.0 6 460 15 0 1 264 445 0.0 6 431 14 265 328 0.0 6 305 22 1 266 653 0.0 6 626 26 1 267 616 0.0 6 604 12 268 372 0.0 6 343 28 1 269 1382 0.0 6 1344 36 2 270 616 0.0 6 591 23 1 0 0 0 1 271 1137 0.0 6 1109 27 1 272 585 0.0 6 572 12 1 273 846 0.0 6 807 38 0 1 274 1419 0.0 6 1379 40 275 1142 0.0 6 1106 35 1 276 1109 0.0 6 1063 44 0 2 277 2920 0.0 6 2848 66 5 1 278 2413 0.0 6 2269 138 6 279 12028 0.0 6 11727 292 8 1 280 28869 0.0 6 28189 670 8 2 281 13127 0.0 6 12891 227 8 0 1 282 16 0.0 6 10 2 2 2 283 6 0.0 6 6 284 2 0.0 6 1 0 1 285 2 0.0 6 1 0 0 0 1 286 2 0.0 6 2 288 1 0.0 6 0 1 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 98 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 5 457.1 1 5 66 1 0.0 6 1 67 1 0.0 6 1 69 2 0.0 6 2 70 8 0.0 6 8 71 6 0.0 6 6 72 4 0.0 6 4 73 3 0.0 6 3 74 2 0.0 6 2 90 2 0.0 6 2 91 5 0.0 6 5 97 2 0.0 6 2 98 2 0.0 6 2 99 1 0.0 6 1 132 1 0.0 6 1 133 3 0.0 6 3 135 3 0.0 6 3 136 3 0.0 6 3 137 1 0.0 6 1 143 4 0.0 6 4 144 5 0.0 6 5 153 2 0.0 6 2 154 6 0.0 6 6 155 2 0.0 6 2 157 2 0.0 6 2 158 6 0.0 6 6 159 1 0.0 6 1 203 1 0.0 6 1 204 1 0.0 6 1 274 4 0.0 6 4 275 8 0.0 6 8 276 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 116846 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1828.3 0 2 4 1 457.1 1 1 6 5 28.6 1 4 1 7 3 7.1 2 2 1 9 1 0.4 2 1 10 1 0.1 3 1 11 4 0.0 3 3 1 12 14 0.0 3 12 2 13 13 0.0 3 9 3 1 14 11 0.0 4 9 2 15 3 0.0 4 3 16 9 0.0 4 8 1 17 27 0.0 5 23 3 1 18 6 0.0 5 4 2 19 51 0.0 5 38 11 2 20 129 0.0 6 124 5 21 119 0.0 6 105 13 1 22 80 0.0 6 71 8 1 23 66 0.0 6 62 3 1 24 43 0.0 6 38 5 25 26 0.0 6 25 1 26 28 0.0 6 27 0 0 0 1 27 23 0.0 6 21 1 0 0 1 28 22 0.0 6 20 2 29 26 0.0 6 23 3 30 25 0.0 6 21 4 31 23 0.0 6 21 1 0 0 0 1 32 17 0.0 6 17 33 30 0.0 6 26 4 34 23 0.0 6 22 0 1 35 21 0.0 6 21 36 17 0.0 6 15 1 0 1 37 15 0.0 6 15 38 26 0.0 6 24 2 39 15 0.0 6 14 1 40 10 0.0 6 10 41 11 0.0 6 10 0 1 42 13 0.0 6 12 1 43 11 0.0 6 10 1 44 29 0.0 6 26 2 0 0 0 1 45 10 0.0 6 9 1 46 40 0.0 6 36 4 47 33 0.0 6 29 4 48 28 0.0 6 24 3 0 0 1 49 41 0.0 6 37 3 1 50 31 0.0 6 25 5 0 0 1 51 32 0.0 6 28 3 1 52 41 0.0 6 36 5 53 21 0.0 6 17 4 54 30 0.0 6 25 3 0 2 55 49 0.0 6 44 3 2 56 51 0.0 6 44 5 1 0 1 57 57 0.0 6 50 6 1 58 29 0.0 6 28 1 59 23 0.0 6 17 4 0 0 2 60 43 0.0 6 37 5 1 61 19 0.0 6 18 1 62 25 0.0 6 22 3 63 27 0.0 6 25 2 64 15 0.0 6 13 2 65 10 0.0 6 7 3 66 20 0.0 6 18 2 67 29 0.0 6 26 2 1 68 32 0.0 6 29 2 1 69 58 0.0 6 49 8 1 70 196 0.0 6 186 10 71 50 0.0 6 44 4 0 0 1 1 72 144 0.0 6 134 10 73 33 0.0 6 31 0 2 74 46 0.0 6 43 3 75 30 0.0 6 26 2 2 76 43 0.0 6 37 6 77 29 0.0 6 24 4 0 0 1 78 38 0.0 6 36 2 79 27 0.0 6 24 2 1 80 41 0.0 6 35 5 1 81 19 0.0 6 18 1 82 35 0.0 6 32 3 83 61 0.0 6 57 4 84 91 0.0 6 85 6 85 80 0.0 6 77 3 86 106 0.0 6 96 9 1 87 99 0.0 6 88 10 1 88 35 0.0 6 28 6 0 0 0 1 89 47 0.0 6 43 3 1 90 80 0.0 6 80 91 52 0.0 6 46 6 92 43 0.0 6 40 3 93 65 0.0 6 59 5 1 94 163 0.0 6 154 9 95 56 0.0 6 54 2 96 108 0.0 6 99 8 1 97 80 0.0 6 72 6 0 0 2 98 51 0.0 6 47 3 0 0 0 1 99 127 0.0 6 113 12 1 0 1 100 86 0.0 6 81 4 1 101 87 0.0 6 81 6 102 188 0.0 6 172 13 1 0 1 1 103 160 0.0 6 149 9 1 0 1 104 133 0.0 6 120 13 105 144 0.0 6 132 10 0 0 2 106 91 0.0 6 87 3 0 1 107 139 0.0 6 132 6 1 108 103 0.0 6 97 6 109 121 0.0 6 114 7 110 107 0.0 6 96 11 111 149 0.0 6 140 8 0 0 1 112 164 0.0 6 148 15 0 1 113 119 0.0 6 108 9 2 114 129 0.0 6 119 9 1 115 141 0.0 6 132 8 0 0 1 116 91 0.0 6 88 3 117 70 0.0 6 68 2 118 86 0.0 6 83 3 119 133 0.0 6 123 8 1 0 1 120 145 0.0 6 141 3 1 121 85 0.0 6 79 5 0 0 1 122 117 0.0 6 108 8 1 123 123 0.0 6 111 12 124 137 0.0 6 127 9 0 0 1 125 79 0.0 6 73 6 126 59 0.0 6 53 6 127 106 0.0 6 103 3 128 109 0.0 6 102 7 129 111 0.0 6 100 10 0 1 130 74 0.0 6 70 4 131 115 0.0 6 110 3 2 132 148 0.0 6 144 4 133 126 0.0 6 123 3 134 215 0.0 6 202 13 135 429 0.0 6 413 14 2 136 304 0.0 6 294 10 137 406 0.0 6 381 20 2 0 3 138 339 0.0 6 330 9 139 491 0.0 6 471 19 1 140 311 0.0 6 292 18 0 0 1 141 281 0.0 6 269 12 142 479 0.0 6 457 20 1 0 1 143 376 0.0 6 361 13 1 1 144 556 0.0 6 526 29 0 0 1 145 313 0.0 6 300 13 146 266 0.0 6 251 14 1 147 155 0.0 6 149 6 148 234 0.0 6 221 12 1 149 479 0.0 6 456 20 1 0 2 150 607 0.0 6 581 24 1 0 1 151 342 0.0 6 317 24 0 0 1 152 760 0.0 6 719 37 2 0 2 153 705 0.0 6 678 26 1 154 705 0.0 6 681 21 1 0 1 1 155 700 0.0 6 678 19 2 1 156 599 0.0 6 579 20 157 685 0.0 6 652 31 1 0 1 158 850 0.0 6 823 27 159 447 0.0 6 429 17 0 0 1 160 820 0.0 6 791 27 1 0 1 161 581 0.0 6 562 18 0 0 1 162 426 0.0 6 407 16 2 1 163 741 0.0 6 710 28 2 0 1 164 657 0.0 6 635 20 1 1 165 888 0.0 6 857 30 1 166 977 0.0 6 955 21 1 167 495 0.0 6 469 25 1 168 1049 0.0 6 1016 31 1 0 1 169 381 0.0 6 363 15 1 0 2 170 413 0.0 6 395 17 0 0 1 171 895 0.0 6 857 36 1 0 1 172 424 0.0 6 409 15 173 736 0.0 6 716 20 174 972 0.0 6 937 33 1 1 175 2142 0.0 6 2064 72 3 0 3 176 1063 0.0 6 1020 39 3 0 1 177 1164 0.0 6 1114 47 1 1 0 1 178 841 0.0 6 808 32 0 0 1 179 1018 0.0 6 974 40 2 0 2 180 1034 0.0 6 987 45 1 1 181 1525 0.0 6 1458 60 6 0 1 182 752 0.0 6 710 40 2 183 1437 0.0 6 1384 50 2 1 184 368 0.0 6 348 13 4 1 2 185 388 0.0 6 361 21 2 2 2 186 1559 0.0 6 1505 51 2 0 1 187 2062 0.0 6 1982 77 2 0 1 188 2538 0.0 6 2444 88 4 0 0 1 1 189 1968 0.0 6 1887 71 6 2 2 190 3114 0.0 6 3001 108 1 0 3 1 191 2445 0.0 6 2365 80 192 1778 0.0 6 1709 68 1 193 1251 0.0 6 1197 45 5 0 4 194 1875 0.0 6 1810 53 6 2 4 195 2143 0.0 6 2049 88 5 1 196 5490 0.0 6 5331 145 5 0 8 1 197 1259 0.0 6 1200 54 3 0 2 198 2489 0.0 6 2381 104 2 0 2 199 5915 0.0 6 5750 157 3 1 4 200 1963 0.0 6 1891 67 2 1 2 201 2759 0.0 6 2668 85 4 0 2 202 1754 0.0 6 1708 44 1 1 203 1374 0.0 6 1319 52 2 1 204 1514 0.0 6 1465 47 2 205 803 0.0 6 777 24 1 0 1 206 717 0.0 6 693 24 207 492 0.0 6 475 15 0 0 2 208 925 0.0 6 888 31 3 2 1 209 666 0.0 6 650 16 210 674 0.0 6 651 22 0 0 1 211 896 0.0 6 874 21 1 212 955 0.0 6 928 26 1 213 223 0.0 6 216 7 214 581 0.0 6 568 10 2 0 1 215 533 0.0 6 508 25 216 793 0.0 6 758 34 1 217 1921 0.0 6 1867 47 4 1 2 218 1005 0.0 6 961 43 0 0 1 219 1267 0.0 6 1229 37 0 0 1 220 766 0.0 6 736 26 0 2 2 221 1626 0.0 6 1579 45 1 0 1 222 540 0.0 6 516 23 1 223 1100 0.0 6 1061 39 224 912 0.0 6 883 28 0 0 1 225 1298 0.0 6 1265 32 1 226 1137 0.0 6 1104 30 1 0 2 227 1061 0.0 6 1033 26 1 0 1 228 523 0.0 6 508 14 0 1 229 943 0.0 6 912 29 0 0 1 0 1 230 669 0.0 6 649 20 231 656 0.0 6 634 22 232 783 0.0 6 759 22 0 0 2 233 678 0.0 6 656 21 0 0 0 0 1 234 486 0.0 6 473 11 1 0 1 235 407 0.0 6 396 9 2 236 155 0.0 6 152 3 237 204 0.0 6 199 4 0 1 238 90 0.0 6 85 5 239 72 0.0 6 69 2 1 240 151 0.0 6 146 4 1 241 326 0.0 6 315 11 242 417 0.0 6 408 8 1 243 113 0.0 6 111 2 244 435 0.0 6 423 11 0 0 1 245 258 0.0 6 250 8 246 194 0.0 6 190 4 247 144 0.0 6 138 4 2 248 170 0.0 6 165 4 1 249 164 0.0 6 156 7 1 250 77 0.0 6 75 2 251 166 0.0 6 163 3 252 40 0.0 6 39 1 253 27 0.0 6 25 1 1 254 127 0.0 6 122 5 255 22 0.0 6 21 1 256 6 0.0 6 6 257 4 0.0 6 4 258 3 0.0 6 3 259 4 0.0 6 4 260 2 0.0 6 2 265 1 0.0 6 1 267 1 0.0 6 1 277 1 0.0 6 1 280 3 0.0 6 3 281 41 0.0 6 37 4 282 77 0.0 6 74 3 285 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 94 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 50 1 0.0 6 1 56 1 0.0 6 1 69 8 0.0 6 8 70 6 0.0 6 6 71 2 0.0 6 2 72 3 0.0 6 3 73 3 0.0 6 3 74 2 0.0 6 2 84 1 0.0 6 1 85 6 0.0 6 6 88 1 0.0 6 1 89 1 0.0 6 1 90 5 0.0 6 5 91 1 0.0 6 1 96 1 0.0 6 1 97 4 0.0 6 4 107 1 0.0 6 1 132 1 0.0 6 1 133 2 0.0 6 2 134 4 0.0 6 4 135 5 0.0 6 5 141 1 0.0 6 1 142 1 0.0 6 1 143 7 0.0 6 7 152 2 0.0 6 2 153 13 0.0 6 13 156 3 0.0 6 3 157 7 0.0 6 7 158 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_18_93_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_18_320_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_18_93_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_18_320_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.38 s (30 µs/read; 1.97 M reads/minute). === Summary === Total read pairs processed: 78,219 Read 1 with adapter: 78,219 (100.0%) Read 2 with adapter: 78,181 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 38 (0.0%) Pairs written (passing filters): 78,181 (100.0%) Total basepairs processed: 39,895,548 bp Read 1: 22,369,965 bp Read 2: 17,525,583 bp Total written (filtered): 4,846,400 bp (12.1%) Read 1: 2,345,254 bp Read 2: 2,501,146 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 78207 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 305.5 1 1 6 2 19.1 1 0 0 2 7 1 4.8 2 1 8 1 1.2 2 1 9 2 0.3 2 2 12 1 0.0 3 1 15 2 0.0 4 2 16 1 0.0 4 0 1 20 17 0.0 6 14 3 21 10 0.0 6 9 1 22 5 0.0 6 5 23 3 0.0 6 3 24 2 0.0 6 2 25 11 0.0 6 10 1 26 4 0.0 6 4 27 1 0.0 6 1 28 2 0.0 6 1 1 30 1 0.0 6 1 32 2 0.0 6 2 33 3 0.0 6 3 34 6 0.0 6 6 35 4 0.0 6 4 36 2 0.0 6 2 37 6 0.0 6 6 38 4 0.0 6 4 39 2 0.0 6 2 41 1 0.0 6 1 42 1 0.0 6 1 44 3 0.0 6 3 45 6 0.0 6 6 46 6 0.0 6 6 47 4 0.0 6 4 49 3 0.0 6 3 50 1 0.0 6 1 52 1 0.0 6 0 0 0 0 1 53 4 0.0 6 3 1 54 9 0.0 6 8 1 55 2 0.0 6 1 1 56 3 0.0 6 2 0 1 57 6 0.0 6 6 58 6 0.0 6 6 59 2 0.0 6 2 60 4 0.0 6 3 1 61 1 0.0 6 1 62 3 0.0 6 3 64 3 0.0 6 3 65 1 0.0 6 1 66 2 0.0 6 2 67 1 0.0 6 1 68 2 0.0 6 2 72 3 0.0 6 3 73 3 0.0 6 3 76 2 0.0 6 2 77 5 0.0 6 5 78 13 0.0 6 12 1 79 3 0.0 6 3 81 3 0.0 6 3 83 3 0.0 6 3 84 2 0.0 6 2 85 1 0.0 6 1 86 9 0.0 6 9 87 3 0.0 6 3 88 1 0.0 6 1 89 5 0.0 6 5 91 2 0.0 6 1 1 92 6 0.0 6 3 2 1 93 2 0.0 6 1 1 94 1 0.0 6 1 97 10 0.0 6 10 98 5 0.0 6 5 99 1 0.0 6 1 100 2 0.0 6 2 101 15 0.0 6 14 1 102 5 0.0 6 5 103 7 0.0 6 7 104 5 0.0 6 4 1 105 5 0.0 6 5 106 5 0.0 6 5 107 3 0.0 6 3 108 14 0.0 6 13 1 109 8 0.0 6 8 110 2 0.0 6 2 111 1 0.0 6 1 112 8 0.0 6 7 1 113 8 0.0 6 7 1 114 10 0.0 6 10 115 10 0.0 6 8 2 116 1 0.0 6 1 117 7 0.0 6 7 118 3 0.0 6 3 119 7 0.0 6 6 1 120 1 0.0 6 1 121 1 0.0 6 1 122 11 0.0 6 10 0 0 0 0 1 123 15 0.0 6 15 124 11 0.0 6 11 125 11 0.0 6 11 126 17 0.0 6 15 2 127 6 0.0 6 6 128 48 0.0 6 44 2 1 1 129 14 0.0 6 13 1 130 9 0.0 6 7 1 0 0 0 1 131 8 0.0 6 8 132 12 0.0 6 11 1 133 57 0.0 6 57 134 104 0.0 6 103 1 135 53 0.0 6 50 3 136 33 0.0 6 32 1 137 20 0.0 6 20 138 8 0.0 6 7 1 139 14 0.0 6 14 140 11 0.0 6 11 141 31 0.0 6 31 142 17 0.0 6 17 143 36 0.0 6 36 144 22 0.0 6 22 145 29 0.0 6 28 1 146 10 0.0 6 10 147 14 0.0 6 14 148 19 0.0 6 19 149 20 0.0 6 18 2 150 26 0.0 6 22 4 151 12 0.0 6 11 1 152 12 0.0 6 12 153 27 0.0 6 27 154 13 0.0 6 13 155 23 0.0 6 23 156 17 0.0 6 12 5 157 67 0.0 6 67 158 13 0.0 6 12 1 159 11 0.0 6 11 160 10 0.0 6 8 2 161 19 0.0 6 18 1 162 19 0.0 6 17 2 163 19 0.0 6 18 1 164 14 0.0 6 13 1 165 29 0.0 6 29 166 25 0.0 6 25 167 46 0.0 6 43 3 168 39 0.0 6 39 169 11 0.0 6 10 0 0 1 170 23 0.0 6 21 2 171 67 0.0 6 63 4 172 33 0.0 6 31 2 173 41 0.0 6 40 1 174 28 0.0 6 27 1 175 26 0.0 6 24 2 176 30 0.0 6 30 177 25 0.0 6 25 178 20 0.0 6 19 1 179 39 0.0 6 38 1 180 83 0.0 6 81 2 181 45 0.0 6 44 1 182 42 0.0 6 42 183 27 0.0 6 25 2 184 52 0.0 6 49 2 1 185 69 0.0 6 65 4 186 50 0.0 6 49 1 187 70 0.0 6 68 2 188 75 0.0 6 75 189 105 0.0 6 103 2 190 106 0.0 6 102 4 191 159 0.0 6 153 5 1 192 112 0.0 6 111 1 193 78 0.0 6 75 3 194 90 0.0 6 87 3 195 79 0.0 6 77 2 196 104 0.0 6 103 1 197 63 0.0 6 61 2 198 91 0.0 6 91 199 124 0.0 6 121 3 200 137 0.0 6 136 1 201 146 0.0 6 143 2 0 0 1 202 135 0.0 6 130 5 203 102 0.0 6 100 2 204 106 0.0 6 103 2 1 205 190 0.0 6 185 4 0 0 1 206 251 0.0 6 245 6 207 201 0.0 6 194 7 208 482 0.0 6 469 13 209 281 0.0 6 273 8 210 268 0.0 6 261 7 211 494 0.0 6 486 8 212 106 0.0 6 96 8 1 1 213 627 0.0 6 615 11 0 0 1 214 145 0.0 6 140 4 1 215 223 0.0 6 219 4 216 159 0.0 6 153 6 217 271 0.0 6 265 6 218 158 0.0 6 153 5 219 247 0.0 6 243 4 220 215 0.0 6 207 8 221 481 0.0 6 478 3 222 189 0.0 6 180 9 223 707 0.0 6 699 7 1 224 320 0.0 6 306 14 225 466 0.0 6 452 14 226 545 0.0 6 533 12 227 391 0.0 6 383 8 228 356 0.0 6 346 10 229 251 0.0 6 243 7 1 230 196 0.0 6 194 2 231 195 0.0 6 191 4 232 184 0.0 6 180 4 233 229 0.0 6 221 8 234 310 0.0 6 307 3 235 215 0.0 6 207 8 236 318 0.0 6 304 13 1 237 1212 0.0 6 1191 21 238 226 0.0 6 221 4 1 239 376 0.0 6 369 6 1 240 295 0.0 6 290 5 241 249 0.0 6 246 3 242 251 0.0 6 247 4 243 526 0.0 6 507 19 244 1077 0.0 6 1056 21 245 340 0.0 6 330 9 1 246 237 0.0 6 230 7 247 599 0.0 6 586 13 248 549 0.0 6 533 16 249 1668 0.0 6 1642 26 250 677 0.0 6 655 21 1 251 636 0.0 6 624 10 2 252 958 0.0 6 933 24 1 253 1171 0.0 6 1133 36 1 1 254 1757 0.0 6 1724 32 1 255 807 0.0 6 781 25 0 1 256 2807 0.0 6 2762 44 1 257 792 0.0 6 760 32 258 1605 0.0 6 1581 24 259 1004 0.0 6 974 29 1 260 2916 0.0 6 2863 50 3 261 859 0.0 6 839 20 262 605 0.0 6 591 12 1 0 0 0 1 263 787 0.0 6 772 14 0 1 264 1474 0.0 6 1445 26 3 265 739 0.0 6 719 19 1 266 1565 0.0 6 1546 19 267 1023 0.0 6 993 30 268 1751 0.0 6 1723 28 269 516 0.0 6 510 5 1 270 527 0.0 6 514 13 271 778 0.0 6 766 11 1 272 788 0.0 6 773 14 0 1 273 1460 0.0 6 1434 26 274 1389 0.0 6 1358 30 1 275 1997 0.0 6 1956 38 2 1 276 2753 0.0 6 2708 39 5 1 277 2029 0.0 6 1974 52 2 0 0 0 1 278 2632 0.0 6 2573 56 3 279 2572 0.0 6 2504 65 1 2 280 10625 0.0 6 10397 222 5 0 0 0 1 281 4704 0.0 6 4487 213 3 0 0 0 1 282 136 0.0 6 110 23 0 3 283 4 0.0 6 4 284 3 0.0 6 1 1 0 0 1 285 6 0.0 6 4 1 1 286 1 0.0 6 1 287 1 0.0 6 1 288 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 12 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 5 305.5 1 5 71 1 0.0 6 1 84 1 0.0 6 1 114 1 0.0 6 1 135 1 0.0 6 1 136 1 0.0 6 1 199 1 0.0 6 1 220 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 78175 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 4.8 2 1 8 1 1.2 2 1 10 5 0.1 3 4 0 1 11 3 0.0 3 3 13 12 0.0 3 11 1 14 4 0.0 4 3 1 15 3 0.0 4 2 1 16 10 0.0 4 9 1 17 28 0.0 5 22 6 18 10 0.0 5 8 2 19 15 0.0 5 14 1 20 35 0.0 6 34 0 1 21 50 0.0 6 49 1 22 40 0.0 6 39 1 23 24 0.0 6 22 2 24 13 0.0 6 12 1 25 24 0.0 6 17 7 26 13 0.0 6 12 1 27 13 0.0 6 11 2 28 5 0.0 6 4 1 29 16 0.0 6 15 1 30 14 0.0 6 13 1 31 15 0.0 6 13 2 32 11 0.0 6 11 33 10 0.0 6 10 34 15 0.0 6 14 0 1 35 8 0.0 6 5 3 36 6 0.0 6 5 0 1 37 15 0.0 6 14 0 1 38 3 0.0 6 3 39 10 0.0 6 8 2 40 1 0.0 6 0 1 41 11 0.0 6 10 1 42 8 0.0 6 6 1 0 0 1 43 8 0.0 6 7 1 44 26 0.0 6 24 2 45 10 0.0 6 9 1 46 19 0.0 6 17 1 0 0 1 47 13 0.0 6 11 2 48 13 0.0 6 10 2 1 49 9 0.0 6 8 0 0 0 1 50 16 0.0 6 14 2 51 10 0.0 6 10 52 20 0.0 6 18 1 0 0 1 53 19 0.0 6 18 1 54 26 0.0 6 24 2 55 19 0.0 6 15 4 56 11 0.0 6 10 1 57 22 0.0 6 18 4 58 14 0.0 6 11 1 1 0 1 59 7 0.0 6 6 1 60 16 0.0 6 11 4 1 61 11 0.0 6 9 2 62 12 0.0 6 12 63 8 0.0 6 7 1 64 12 0.0 6 10 2 65 3 0.0 6 3 66 27 0.0 6 21 4 1 1 67 144 0.0 6 136 8 68 38 0.0 6 37 1 69 54 0.0 6 54 70 13 0.0 6 12 0 0 0 1 71 32 0.0 6 27 5 72 25 0.0 6 23 2 73 7 0.0 6 6 1 74 12 0.0 6 11 1 75 15 0.0 6 12 3 76 27 0.0 6 25 1 1 77 13 0.0 6 10 2 1 78 24 0.0 6 19 5 79 13 0.0 6 13 80 13 0.0 6 12 1 81 12 0.0 6 11 0 1 82 30 0.0 6 25 5 83 60 0.0 6 53 6 1 84 40 0.0 6 37 3 85 17 0.0 6 16 1 86 21 0.0 6 18 2 1 87 42 0.0 6 40 2 88 21 0.0 6 19 1 1 89 24 0.0 6 23 1 90 31 0.0 6 30 1 91 33 0.0 6 30 3 92 17 0.0 6 15 2 93 47 0.0 6 43 4 94 46 0.0 6 43 2 0 1 95 37 0.0 6 34 3 96 30 0.0 6 27 2 1 97 28 0.0 6 25 3 98 29 0.0 6 25 3 0 1 99 48 0.0 6 42 6 100 44 0.0 6 40 2 1 1 101 49 0.0 6 44 5 102 62 0.0 6 54 6 2 103 65 0.0 6 61 4 104 59 0.0 6 53 5 1 105 66 0.0 6 59 4 3 106 29 0.0 6 28 1 107 49 0.0 6 45 4 108 43 0.0 6 39 3 1 109 38 0.0 6 38 110 43 0.0 6 38 4 0 0 1 111 46 0.0 6 39 4 1 2 112 23 0.0 6 22 1 113 41 0.0 6 35 4 0 0 1 0 1 114 62 0.0 6 57 4 1 115 43 0.0 6 40 3 116 45 0.0 6 44 1 117 29 0.0 6 28 1 118 39 0.0 6 36 3 119 44 0.0 6 38 5 0 1 120 64 0.0 6 59 5 121 39 0.0 6 35 4 122 38 0.0 6 35 3 123 38 0.0 6 37 0 0 0 0 1 124 62 0.0 6 60 2 125 55 0.0 6 49 6 126 60 0.0 6 54 3 3 127 68 0.0 6 63 5 128 43 0.0 6 42 1 129 35 0.0 6 30 5 130 41 0.0 6 40 1 131 106 0.0 6 102 4 132 120 0.0 6 111 7 2 133 74 0.0 6 74 134 184 0.0 6 178 5 1 135 238 0.0 6 232 6 136 105 0.0 6 104 0 1 137 140 0.0 6 136 4 138 56 0.0 6 55 1 139 130 0.0 6 127 2 0 0 0 1 140 266 0.0 6 249 16 1 141 173 0.0 6 160 13 142 259 0.0 6 248 10 1 143 219 0.0 6 205 13 0 1 144 365 0.0 6 350 14 1 145 186 0.0 6 180 5 0 0 1 146 202 0.0 6 194 7 0 0 1 147 119 0.0 6 117 2 148 235 0.0 6 217 17 1 149 326 0.0 6 311 12 1 1 0 1 150 576 0.0 6 548 25 1 1 1 151 240 0.0 6 229 10 0 0 1 152 424 0.0 6 413 11 153 297 0.0 6 281 15 1 154 288 0.0 6 275 12 0 0 1 155 201 0.0 6 191 8 2 156 251 0.0 6 238 11 1 1 157 355 0.0 6 339 14 1 1 158 411 0.0 6 397 14 159 221 0.0 6 212 9 160 390 0.0 6 373 17 161 189 0.0 6 180 6 1 0 1 1 162 147 0.0 6 138 8 0 0 0 0 1 163 356 0.0 6 344 10 2 164 366 0.0 6 354 12 165 426 0.0 6 411 15 166 599 0.0 6 578 15 1 1 3 0 1 167 346 0.0 6 331 15 168 774 0.0 6 749 25 169 343 0.0 6 335 7 1 170 473 0.0 6 458 13 0 0 2 171 675 0.0 6 658 16 1 172 226 0.0 6 216 9 1 173 360 0.0 6 351 8 1 174 651 0.0 6 624 25 2 175 976 0.0 6 948 26 1 0 0 1 176 703 0.0 6 675 27 1 177 840 0.0 6 815 23 2 178 669 0.0 6 648 20 1 179 751 0.0 6 722 26 1 1 1 180 620 0.0 6 598 20 2 181 732 0.0 6 714 14 3 1 182 149 0.0 6 142 7 183 804 0.0 6 785 19 184 790 0.0 6 766 22 1 0 1 185 980 0.0 6 938 40 1 1 186 1852 0.0 6 1803 42 4 2 1 187 1607 0.0 6 1560 43 1 0 3 188 1954 0.0 6 1909 43 2 189 842 0.0 6 823 17 0 0 2 190 1426 0.0 6 1386 36 3 1 191 1353 0.0 6 1307 44 2 192 959 0.0 6 921 38 193 2038 0.0 6 1994 41 1 1 1 194 686 0.0 6 670 15 0 0 1 195 1750 0.0 6 1711 38 1 196 677 0.0 6 656 18 0 0 2 1 197 1493 0.0 6 1443 45 2 0 1 1 1 198 2161 0.0 6 2098 59 0 0 4 199 1636 0.0 6 1597 37 1 1 200 1402 0.0 6 1356 45 1 201 1484 0.0 6 1448 36 202 1319 0.0 6 1281 33 3 0 2 203 1111 0.0 6 1072 37 2 204 1140 0.0 6 1108 31 1 205 1888 0.0 6 1829 55 3 1 206 293 0.0 6 280 11 2 207 933 0.0 6 907 24 1 0 1 208 1219 0.0 6 1189 29 1 209 890 0.0 6 872 16 1 0 1 210 291 0.0 6 279 10 1 1 211 873 0.0 6 856 15 1 0 1 212 1108 0.0 6 1084 23 0 0 1 213 997 0.0 6 969 25 2 0 1 214 639 0.0 6 618 21 215 499 0.0 6 486 12 1 216 509 0.0 6 494 12 0 1 1 0 1 217 294 0.0 6 286 8 218 380 0.0 6 368 12 219 382 0.0 6 375 7 220 279 0.0 6 274 5 221 413 0.0 6 396 16 0 0 1 222 384 0.0 6 369 12 3 223 703 0.0 6 680 21 1 0 1 224 1252 0.0 6 1223 28 0 0 1 225 821 0.0 6 803 17 1 226 898 0.0 6 860 34 1 2 0 0 1 227 1714 0.0 6 1666 44 0 0 4 228 716 0.0 6 692 23 0 1 229 2009 0.0 6 1965 44 230 225 0.0 6 220 5 231 741 0.0 6 724 17 232 582 0.0 6 572 9 0 1 233 341 0.0 6 335 5 1 234 491 0.0 6 473 18 235 204 0.0 6 191 9 2 1 1 236 43 0.0 6 41 2 237 103 0.0 6 95 8 238 230 0.0 6 222 8 239 130 0.0 6 126 3 1 240 49 0.0 6 48 1 241 134 0.0 6 129 5 242 485 0.0 6 474 11 243 233 0.0 6 224 9 244 256 0.0 6 247 9 245 139 0.0 6 137 2 246 271 0.0 6 265 6 247 155 0.0 6 149 6 248 441 0.0 6 426 15 249 212 0.0 6 205 6 0 1 250 51 0.0 6 49 2 251 478 0.0 6 468 10 252 87 0.0 6 87 253 43 0.0 6 42 1 254 6 0.0 6 6 255 5 0.0 6 5 257 2 0.0 6 2 261 1 0.0 6 1 268 1 0.0 6 1 269 1 0.0 6 1 276 2 0.0 6 2 277 15 0.0 6 15 278 41 0.0 6 41 279 293 0.0 6 290 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 6 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 74 2 0.0 6 2 116 2 0.0 6 2 201 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_19_94_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_19_321_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_19_94_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_19_321_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.56 s (41 µs/read; 1.46 M reads/minute). === Summary === Total read pairs processed: 62,386 Read 1 with adapter: 62,386 (100.0%) Read 2 with adapter: 62,362 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 24 (0.0%) Pairs written (passing filters): 62,362 (100.0%) Total basepairs processed: 32,622,374 bp Read 1: 18,154,458 bp Read 2: 14,467,916 bp Total written (filtered): 3,742,414 bp (11.5%) Read 1: 1,870,906 bp Read 2: 1,871,508 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 62379 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 8 1 1.0 2 0 1 9 2 0.2 2 0 2 20 10 0.0 6 10 21 7 0.0 6 7 22 5 0.0 6 5 23 3 0.0 6 3 24 4 0.0 6 4 25 3 0.0 6 3 26 4 0.0 6 4 27 3 0.0 6 3 33 2 0.0 6 2 34 1 0.0 6 1 36 1 0.0 6 0 0 1 37 1 0.0 6 1 39 2 0.0 6 2 40 4 0.0 6 4 41 1 0.0 6 1 42 1 0.0 6 1 44 1 0.0 6 1 45 27 0.0 6 27 46 6 0.0 6 6 47 1 0.0 6 1 48 1 0.0 6 1 50 1 0.0 6 1 51 3 0.0 6 3 52 2 0.0 6 2 54 1 0.0 6 1 55 5 0.0 6 5 56 1 0.0 6 1 57 1 0.0 6 1 58 2 0.0 6 2 60 2 0.0 6 2 61 2 0.0 6 2 63 2 0.0 6 2 64 3 0.0 6 3 65 2 0.0 6 2 66 1 0.0 6 1 67 1 0.0 6 1 69 1 0.0 6 0 0 1 70 2 0.0 6 1 1 71 1 0.0 6 1 74 1 0.0 6 1 75 1 0.0 6 1 76 1 0.0 6 0 1 78 2 0.0 6 2 83 2 0.0 6 2 84 1 0.0 6 1 87 8 0.0 6 7 1 88 4 0.0 6 4 89 1 0.0 6 1 90 1 0.0 6 1 91 1 0.0 6 1 93 5 0.0 6 4 1 94 1 0.0 6 1 96 1 0.0 6 1 98 2 0.0 6 2 99 4 0.0 6 4 101 2 0.0 6 2 102 6 0.0 6 6 103 5 0.0 6 5 104 1 0.0 6 1 105 4 0.0 6 4 107 7 0.0 6 5 2 108 8 0.0 6 6 2 109 2 0.0 6 2 111 1 0.0 6 1 112 5 0.0 6 5 113 3 0.0 6 3 114 3 0.0 6 3 115 4 0.0 6 4 116 1 0.0 6 1 117 6 0.0 6 5 1 118 1 0.0 6 1 119 1 0.0 6 1 120 4 0.0 6 2 1 1 121 14 0.0 6 14 122 12 0.0 6 12 123 4 0.0 6 4 124 4 0.0 6 4 125 6 0.0 6 5 1 126 9 0.0 6 8 1 127 13 0.0 6 13 128 12 0.0 6 11 1 129 8 0.0 6 8 130 21 0.0 6 20 1 131 4 0.0 6 4 132 11 0.0 6 10 1 133 67 0.0 6 66 1 134 18 0.0 6 18 135 6 0.0 6 5 1 136 4 0.0 6 4 137 2 0.0 6 1 1 138 3 0.0 6 3 139 14 0.0 6 14 140 12 0.0 6 12 141 19 0.0 6 19 142 15 0.0 6 14 1 143 9 0.0 6 9 144 20 0.0 6 19 1 145 29 0.0 6 29 146 23 0.0 6 23 147 13 0.0 6 12 1 148 30 0.0 6 29 1 149 20 0.0 6 20 150 18 0.0 6 18 151 19 0.0 6 19 152 14 0.0 6 13 1 153 20 0.0 6 19 1 154 7 0.0 6 7 155 9 0.0 6 8 1 156 8 0.0 6 8 157 14 0.0 6 14 158 27 0.0 6 27 159 16 0.0 6 16 160 9 0.0 6 9 161 21 0.0 6 20 1 162 28 0.0 6 27 1 163 20 0.0 6 20 164 9 0.0 6 8 1 165 29 0.0 6 28 1 166 14 0.0 6 12 2 167 13 0.0 6 13 168 23 0.0 6 21 1 1 169 11 0.0 6 11 170 11 0.0 6 11 171 32 0.0 6 32 172 34 0.0 6 34 173 13 0.0 6 13 174 28 0.0 6 27 1 175 11 0.0 6 10 1 176 9 0.0 6 9 177 18 0.0 6 18 178 23 0.0 6 21 2 179 25 0.0 6 24 1 180 9 0.0 6 7 2 181 18 0.0 6 18 182 25 0.0 6 25 183 22 0.0 6 22 184 15 0.0 6 13 2 185 27 0.0 6 27 186 28 0.0 6 26 2 187 52 0.0 6 50 2 188 71 0.0 6 68 3 189 66 0.0 6 64 2 190 38 0.0 6 38 191 25 0.0 6 24 1 192 9 0.0 6 9 193 27 0.0 6 25 2 194 35 0.0 6 34 1 195 17 0.0 6 16 1 196 38 0.0 6 38 197 29 0.0 6 29 198 50 0.0 6 50 199 67 0.0 6 62 5 200 62 0.0 6 61 1 201 82 0.0 6 82 202 85 0.0 6 84 1 203 57 0.0 6 56 1 204 47 0.0 6 46 1 205 88 0.0 6 82 6 206 126 0.0 6 122 3 1 207 413 0.0 6 400 13 208 269 0.0 6 259 10 209 196 0.0 6 187 9 210 409 0.0 6 403 6 211 388 0.0 6 376 12 212 289 0.0 6 282 7 213 128 0.0 6 126 2 214 150 0.0 6 149 1 215 62 0.0 6 61 1 216 109 0.0 6 103 6 217 80 0.0 6 79 1 218 164 0.0 6 163 1 219 103 0.0 6 100 3 220 179 0.0 6 174 5 221 137 0.0 6 132 5 222 212 0.0 6 210 2 223 96 0.0 6 95 1 224 75 0.0 6 74 1 225 58 0.0 6 56 1 0 0 0 1 226 107 0.0 6 103 4 227 238 0.0 6 231 7 228 191 0.0 6 187 4 229 79 0.0 6 73 5 1 230 162 0.0 6 156 6 231 356 0.0 6 351 4 1 232 72 0.0 6 70 2 233 146 0.0 6 146 234 211 0.0 6 211 235 99 0.0 6 94 5 236 353 0.0 6 344 8 1 237 64 0.0 6 63 1 238 364 0.0 6 361 3 239 129 0.0 6 128 1 240 65 0.0 6 63 2 241 135 0.0 6 133 2 242 109 0.0 6 106 3 243 147 0.0 6 144 3 244 344 0.0 6 336 7 0 0 1 245 460 0.0 6 447 13 246 269 0.0 6 262 7 247 118 0.0 6 115 3 248 454 0.0 6 444 10 249 484 0.0 6 467 17 250 1088 0.0 6 1063 25 251 516 0.0 6 507 9 252 375 0.0 6 360 15 253 855 0.0 6 837 18 254 1036 0.0 6 1008 26 1 1 255 1560 0.0 6 1531 28 0 0 0 0 1 256 685 0.0 6 670 15 257 1908 0.0 6 1876 32 258 580 0.0 6 564 16 259 1740 0.0 6 1704 34 2 260 1184 0.0 6 1149 34 1 261 2366 0.0 6 2328 38 262 460 0.0 6 447 12 1 263 326 0.0 6 314 12 264 627 0.0 6 608 18 1 265 1276 0.0 6 1246 30 266 444 0.0 6 432 12 267 1265 0.0 6 1246 19 268 386 0.0 6 361 25 269 949 0.0 6 930 19 270 324 0.0 6 315 8 1 271 610 0.0 6 596 14 272 362 0.0 6 344 18 273 341 0.0 6 324 17 274 1475 0.0 6 1448 27 275 1373 0.0 6 1329 43 1 276 2300 0.0 6 2248 52 277 4362 0.0 6 4264 96 2 278 3911 0.0 6 3826 84 1 279 5472 0.0 6 5374 97 1 280 9788 0.0 6 9542 243 2 1 281 1310 0.0 6 1236 59 15 282 30 0.0 6 25 5 283 21 0.0 6 19 2 284 2 0.0 6 0 2 285 3 0.0 6 0 1 0 2 286 1 0.0 6 1 288 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 7 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 3 243.7 1 3 76 1 0.0 6 1 77 1 0.0 6 1 86 1 0.0 6 1 105 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 62349 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 9 1 0.2 2 1 11 5 0.0 3 4 0 1 12 3 0.0 3 3 13 9 0.0 3 9 14 2 0.0 4 2 16 9 0.0 4 8 1 17 6 0.0 5 5 1 18 5 0.0 5 5 19 3 0.0 5 2 1 20 25 0.0 6 25 21 39 0.0 6 38 1 22 33 0.0 6 30 3 23 20 0.0 6 18 2 24 15 0.0 6 15 25 13 0.0 6 13 26 9 0.0 6 7 2 27 4 0.0 6 3 1 28 4 0.0 6 2 2 29 9 0.0 6 9 30 9 0.0 6 8 0 0 1 32 5 0.0 6 5 33 2 0.0 6 2 34 8 0.0 6 7 0 1 35 2 0.0 6 2 36 4 0.0 6 4 37 8 0.0 6 8 38 4 0.0 6 4 39 4 0.0 6 4 40 3 0.0 6 3 41 7 0.0 6 7 42 5 0.0 6 5 43 6 0.0 6 5 1 44 22 0.0 6 19 3 45 5 0.0 6 5 46 11 0.0 6 11 47 11 0.0 6 10 1 48 7 0.0 6 7 49 14 0.0 6 14 50 11 0.0 6 10 1 51 6 0.0 6 6 52 10 0.0 6 10 53 7 0.0 6 7 54 9 0.0 6 8 1 55 10 0.0 6 10 56 19 0.0 6 17 1 0 1 57 5 0.0 6 4 1 58 10 0.0 6 9 1 59 12 0.0 6 10 1 0 0 0 1 60 7 0.0 6 7 61 12 0.0 6 8 3 1 62 5 0.0 6 5 63 5 0.0 6 5 64 13 0.0 6 13 65 12 0.0 6 11 1 66 7 0.0 6 6 1 67 13 0.0 6 10 3 68 24 0.0 6 22 2 69 111 0.0 6 107 3 1 70 15 0.0 6 14 1 71 52 0.0 6 47 5 72 28 0.0 6 25 3 73 19 0.0 6 19 74 15 0.0 6 15 75 7 0.0 6 7 76 20 0.0 6 20 77 24 0.0 6 23 1 78 30 0.0 6 29 1 79 17 0.0 6 16 1 80 17 0.0 6 14 3 81 16 0.0 6 15 1 82 14 0.0 6 13 0 0 0 1 83 27 0.0 6 23 3 1 84 40 0.0 6 38 2 85 28 0.0 6 25 3 86 14 0.0 6 12 2 87 8 0.0 6 8 88 1 0.0 6 1 89 13 0.0 6 11 2 90 11 0.0 6 10 1 91 10 0.0 6 10 92 16 0.0 6 15 1 93 15 0.0 6 15 94 53 0.0 6 53 95 33 0.0 6 31 2 96 28 0.0 6 26 2 97 60 0.0 6 54 6 98 33 0.0 6 31 2 99 20 0.0 6 19 0 1 100 24 0.0 6 24 101 16 0.0 6 14 2 102 36 0.0 6 34 2 103 37 0.0 6 37 104 47 0.0 6 45 2 105 55 0.0 6 51 3 0 1 106 27 0.0 6 26 1 107 47 0.0 6 44 2 0 0 1 108 15 0.0 6 13 2 109 7 0.0 6 7 110 31 0.0 6 31 111 9 0.0 6 9 112 9 0.0 6 7 1 1 113 13 0.0 6 13 114 6 0.0 6 6 115 58 0.0 6 54 4 116 83 0.0 6 79 3 0 0 1 117 90 0.0 6 80 7 2 0 1 118 29 0.0 6 27 2 119 65 0.0 6 59 5 0 0 1 120 36 0.0 6 35 1 121 61 0.0 6 57 3 1 122 37 0.0 6 34 2 1 123 102 0.0 6 94 7 1 124 48 0.0 6 47 0 0 0 1 125 43 0.0 6 40 3 126 95 0.0 6 90 3 2 127 136 0.0 6 132 4 128 43 0.0 6 41 1 1 129 66 0.0 6 65 1 130 99 0.0 6 95 4 131 92 0.0 6 87 5 132 25 0.0 6 23 2 133 32 0.0 6 28 2 2 134 62 0.0 6 61 1 135 75 0.0 6 73 2 136 100 0.0 6 97 3 137 58 0.0 6 52 6 138 173 0.0 6 165 7 0 0 1 139 112 0.0 6 107 3 2 140 91 0.0 6 84 7 141 152 0.0 6 147 5 142 100 0.0 6 91 8 0 0 0 0 1 143 208 0.0 6 202 5 0 0 1 144 166 0.0 6 163 3 145 74 0.0 6 68 6 146 145 0.0 6 143 2 147 107 0.0 6 104 3 148 188 0.0 6 184 4 149 139 0.0 6 132 7 150 215 0.0 6 206 9 151 146 0.0 6 141 5 152 206 0.0 6 202 4 153 156 0.0 6 151 4 1 154 229 0.0 6 222 7 155 561 0.0 6 535 24 1 0 1 156 786 0.0 6 769 17 157 498 0.0 6 485 13 158 186 0.0 6 177 9 159 296 0.0 6 287 9 160 130 0.0 6 127 3 161 161 0.0 6 157 4 162 213 0.0 6 207 6 163 352 0.0 6 340 9 2 0 1 164 722 0.0 6 706 15 0 1 165 276 0.0 6 267 8 1 166 439 0.0 6 427 12 167 258 0.0 6 249 8 1 168 171 0.0 6 165 5 1 169 202 0.0 6 194 7 1 170 369 0.0 6 361 8 171 341 0.0 6 330 11 172 587 0.0 6 575 11 1 173 307 0.0 6 295 10 1 0 1 174 733 0.0 6 706 25 0 1 1 175 605 0.0 6 598 7 176 189 0.0 6 183 5 1 177 197 0.0 6 193 3 1 178 194 0.0 6 184 9 0 0 1 179 338 0.0 6 326 12 180 126 0.0 6 120 3 1 0 1 1 181 164 0.0 6 156 7 1 182 175 0.0 6 165 9 1 183 388 0.0 6 377 8 2 0 0 1 184 109 0.0 6 104 4 0 0 0 1 185 324 0.0 6 315 9 186 233 0.0 6 225 7 1 187 740 0.0 6 722 17 1 188 514 0.0 6 495 18 1 189 662 0.0 6 641 21 190 281 0.0 6 270 11 191 398 0.0 6 384 14 192 657 0.0 6 643 14 193 772 0.0 6 753 17 2 194 716 0.0 6 696 19 0 0 1 195 587 0.0 6 578 7 0 1 1 196 394 0.0 6 385 8 0 0 1 197 251 0.0 6 242 9 198 772 0.0 6 742 27 1 0 2 199 1001 0.0 6 977 24 200 1034 0.0 6 1006 26 2 201 694 0.0 6 674 19 1 202 768 0.0 6 748 20 203 658 0.0 6 647 10 0 1 204 610 0.0 6 591 18 0 1 205 494 0.0 6 478 16 206 817 0.0 6 801 16 207 214 0.0 6 205 8 0 1 208 628 0.0 6 611 17 209 456 0.0 6 442 14 210 331 0.0 6 325 6 211 76 0.0 6 71 4 0 1 212 197 0.0 6 191 5 1 213 60 0.0 6 56 3 0 1 214 208 0.0 6 204 3 0 0 1 215 155 0.0 6 155 216 500 0.0 6 482 16 0 2 217 384 0.0 6 375 8 1 218 539 0.0 6 528 10 0 0 0 1 219 629 0.0 6 614 12 2 0 1 220 264 0.0 6 258 5 0 1 221 189 0.0 6 183 4 1 1 222 233 0.0 6 218 12 2 1 223 1009 0.0 6 978 31 224 3707 0.0 6 3636 67 0 2 2 225 1442 0.0 6 1405 30 3 2 1 1 226 1291 0.0 6 1232 57 1 1 227 5694 0.0 6 5582 105 3 0 3 1 228 642 0.0 6 613 24 3 1 1 229 1799 0.0 6 1767 28 2 1 1 230 351 0.0 6 337 13 1 231 2038 0.0 6 1995 41 0 0 2 232 1138 0.0 6 1110 24 2 1 0 1 233 1305 0.0 6 1276 29 234 1800 0.0 6 1758 41 1 235 342 0.0 6 339 3 236 138 0.0 6 135 3 237 158 0.0 6 150 7 0 0 0 1 238 181 0.0 6 177 4 239 78 0.0 6 77 1 240 61 0.0 6 57 4 241 459 0.0 6 449 10 242 123 0.0 6 116 6 1 243 440 0.0 6 429 10 0 0 1 244 748 0.0 6 737 10 1 245 86 0.0 6 81 5 246 289 0.0 6 279 10 247 138 0.0 6 135 3 248 293 0.0 6 283 9 0 1 249 537 0.0 6 523 14 250 150 0.0 6 142 7 1 251 205 0.0 6 198 7 252 389 0.0 6 378 11 253 128 0.0 6 125 3 254 314 0.0 6 308 6 255 201 0.0 6 197 3 0 0 1 256 128 0.0 6 125 3 257 33 0.0 6 31 2 258 3 0.0 6 3 259 1 0.0 6 1 260 1 0.0 6 1 261 1 0.0 6 1 263 4 0.0 6 4 264 1 0.0 6 1 265 5 0.0 6 4 1 266 4 0.0 6 4 267 2 0.0 6 2 269 2 0.0 6 2 270 1 0.0 6 0 1 279 7 0.0 6 7 280 29 0.0 6 28 0 1 281 73 0.0 6 70 3 282 3 0.0 6 2 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 13 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 74 2 0.0 6 2 77 2 0.0 6 2 80 1 0.0 6 1 86 1 0.0 6 1 87 1 0.0 6 1 94 1 0.0 6 1 115 2 0.0 6 0 0 0 0 0 2 117 1 0.0 6 0 0 0 0 0 1 119 1 0.0 6 1 163 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_20_95_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_20_322_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_20_95_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_20_322_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.59 s (42 µs/read; 1.42 M reads/minute). === Summary === Total read pairs processed: 61,363 Read 1 with adapter: 61,363 (100.0%) Read 2 with adapter: 61,315 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 48 (0.1%) Pairs written (passing filters): 61,315 (99.9%) Total basepairs processed: 30,794,371 bp Read 1: 17,825,970 bp Read 2: 12,968,401 bp Total written (filtered): 3,801,711 bp (12.3%) Read 1: 1,839,447 bp Read 2: 1,962,264 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 61345 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 239.7 1 1 5 1 59.9 1 1 7 1 3.7 2 0 0 1 15 1 0.0 4 1 20 11 0.0 6 9 2 21 9 0.0 6 9 22 3 0.0 6 3 23 2 0.0 6 2 24 3 0.0 6 3 25 3 0.0 6 3 26 3 0.0 6 3 27 1 0.0 6 1 28 1 0.0 6 1 29 2 0.0 6 2 30 2 0.0 6 1 1 31 2 0.0 6 1 0 0 1 33 1 0.0 6 0 1 34 6 0.0 6 5 1 35 1 0.0 6 1 38 4 0.0 6 3 0 0 0 0 1 39 2 0.0 6 2 40 2 0.0 6 2 41 5 0.0 6 5 44 4 0.0 6 3 1 45 5 0.0 6 5 46 4 0.0 6 4 47 1 0.0 6 1 48 3 0.0 6 2 1 50 2 0.0 6 1 1 53 2 0.0 6 2 54 3 0.0 6 1 1 1 55 1 0.0 6 0 0 1 56 3 0.0 6 3 57 3 0.0 6 3 58 2 0.0 6 2 59 3 0.0 6 1 2 60 1 0.0 6 1 61 1 0.0 6 1 62 5 0.0 6 5 63 2 0.0 6 2 64 3 0.0 6 1 2 65 3 0.0 6 2 1 67 1 0.0 6 1 68 4 0.0 6 4 69 1 0.0 6 1 70 1 0.0 6 1 71 1 0.0 6 1 72 2 0.0 6 1 1 73 2 0.0 6 2 74 2 0.0 6 2 76 4 0.0 6 4 77 3 0.0 6 3 78 7 0.0 6 6 1 79 2 0.0 6 2 80 1 0.0 6 0 1 81 1 0.0 6 1 85 1 0.0 6 1 86 4 0.0 6 4 87 4 0.0 6 3 1 88 1 0.0 6 1 89 3 0.0 6 3 90 2 0.0 6 2 91 1 0.0 6 1 92 4 0.0 6 4 93 3 0.0 6 3 94 2 0.0 6 2 96 1 0.0 6 1 97 4 0.0 6 4 98 5 0.0 6 5 100 1 0.0 6 1 101 5 0.0 6 4 1 102 4 0.0 6 4 103 3 0.0 6 3 104 2 0.0 6 2 105 1 0.0 6 1 106 9 0.0 6 9 107 3 0.0 6 3 108 6 0.0 6 6 109 5 0.0 6 5 110 1 0.0 6 1 111 4 0.0 6 4 112 2 0.0 6 2 113 2 0.0 6 2 114 5 0.0 6 5 115 3 0.0 6 1 1 1 116 2 0.0 6 2 117 2 0.0 6 2 118 2 0.0 6 1 1 119 7 0.0 6 7 120 3 0.0 6 3 121 8 0.0 6 8 122 12 0.0 6 11 1 123 12 0.0 6 12 124 6 0.0 6 6 125 10 0.0 6 10 126 3 0.0 6 2 1 127 8 0.0 6 7 1 128 25 0.0 6 21 4 129 7 0.0 6 7 130 7 0.0 6 6 1 131 3 0.0 6 2 0 1 132 6 0.0 6 5 1 133 17 0.0 6 17 134 11 0.0 6 11 135 12 0.0 6 12 136 11 0.0 6 11 137 36 0.0 6 35 1 138 25 0.0 6 25 139 13 0.0 6 13 140 15 0.0 6 14 1 141 11 0.0 6 10 1 142 17 0.0 6 17 143 34 0.0 6 33 1 144 69 0.0 6 68 1 145 57 0.0 6 55 2 146 10 0.0 6 10 147 12 0.0 6 11 1 148 34 0.0 6 30 3 1 149 57 0.0 6 55 2 150 42 0.0 6 39 0 3 151 24 0.0 6 23 1 152 16 0.0 6 14 2 153 16 0.0 6 14 2 154 36 0.0 6 36 155 11 0.0 6 11 156 19 0.0 6 19 157 24 0.0 6 21 3 158 18 0.0 6 18 159 29 0.0 6 28 1 160 28 0.0 6 27 1 161 29 0.0 6 28 1 162 44 0.0 6 44 163 41 0.0 6 39 2 164 29 0.0 6 29 165 22 0.0 6 22 166 32 0.0 6 31 1 167 18 0.0 6 18 168 29 0.0 6 28 1 169 14 0.0 6 13 1 170 15 0.0 6 13 2 171 31 0.0 6 30 1 172 14 0.0 6 13 1 173 39 0.0 6 37 2 174 18 0.0 6 18 175 41 0.0 6 41 176 23 0.0 6 23 177 46 0.0 6 44 2 178 34 0.0 6 34 179 33 0.0 6 33 180 6 0.0 6 6 181 38 0.0 6 37 1 182 76 0.0 6 76 183 100 0.0 6 96 4 184 140 0.0 6 135 5 185 121 0.0 6 117 4 186 167 0.0 6 165 1 1 187 96 0.0 6 90 5 1 188 70 0.0 6 64 6 189 55 0.0 6 54 1 190 69 0.0 6 66 3 191 90 0.0 6 87 3 192 89 0.0 6 86 3 193 82 0.0 6 80 2 194 63 0.0 6 61 2 195 86 0.0 6 82 4 196 128 0.0 6 124 4 197 133 0.0 6 132 1 198 174 0.0 6 168 6 199 169 0.0 6 165 4 200 181 0.0 6 178 3 201 181 0.0 6 175 5 1 202 161 0.0 6 156 5 203 234 0.0 6 229 4 1 204 153 0.0 6 146 7 205 235 0.0 6 230 5 206 170 0.0 6 161 8 1 207 323 0.0 6 312 11 208 270 0.0 6 262 7 0 0 0 0 1 209 199 0.0 6 189 10 210 194 0.0 6 192 2 211 114 0.0 6 112 2 212 93 0.0 6 92 1 213 180 0.0 6 176 4 214 199 0.0 6 196 2 1 215 120 0.0 6 115 5 216 144 0.0 6 136 8 217 394 0.0 6 384 10 218 181 0.0 6 178 2 1 219 155 0.0 6 152 3 220 148 0.0 6 141 6 1 221 173 0.0 6 164 8 1 222 239 0.0 6 233 6 223 101 0.0 6 99 2 224 98 0.0 6 95 3 225 188 0.0 6 178 9 1 226 383 0.0 6 369 14 227 410 0.0 6 390 20 228 437 0.0 6 429 7 1 229 319 0.0 6 312 7 230 378 0.0 6 373 5 231 240 0.0 6 230 10 232 445 0.0 6 444 1 233 317 0.0 6 302 14 0 1 234 293 0.0 6 287 6 235 154 0.0 6 149 4 1 236 197 0.0 6 195 2 237 156 0.0 6 148 8 238 204 0.0 6 193 10 1 239 212 0.0 6 204 8 240 348 0.0 6 334 12 2 241 419 0.0 6 400 18 0 1 242 399 0.0 6 391 8 243 311 0.0 6 302 9 244 300 0.0 6 288 12 245 362 0.0 6 356 6 246 133 0.0 6 124 9 247 198 0.0 6 188 10 248 124 0.0 6 118 6 249 389 0.0 6 381 7 1 250 238 0.0 6 230 7 1 251 281 0.0 6 267 14 252 297 0.0 6 290 7 253 208 0.0 6 201 6 0 1 254 486 0.0 6 473 13 255 228 0.0 6 221 7 256 264 0.0 6 258 6 257 188 0.0 6 180 8 258 295 0.0 6 279 16 259 474 0.0 6 458 15 1 260 724 0.0 6 708 15 1 261 245 0.0 6 229 16 262 495 0.0 6 481 14 263 757 0.0 6 747 8 0 2 264 465 0.0 6 444 20 1 265 672 0.0 6 660 12 266 351 0.0 6 338 13 267 412 0.0 6 394 17 1 268 389 0.0 6 372 16 0 1 269 398 0.0 6 390 8 270 177 0.0 6 164 13 271 592 0.0 6 551 39 1 0 1 272 925 0.0 6 908 15 2 273 525 0.0 6 514 10 1 274 575 0.0 6 555 20 275 743 0.0 6 712 29 2 276 1257 0.0 6 1231 26 277 996 0.0 6 977 19 278 409 0.0 6 394 13 2 279 768 0.0 6 747 19 1 1 280 519 0.0 6 428 82 6 3 281 30448 0.0 6 29985 455 7 1 282 10 0.0 6 8 2 283 4 0.0 6 4 284 1 0.0 6 1 289 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 18 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 11 239.7 1 11 71 1 0.0 6 1 72 1 0.0 6 1 74 1 0.0 6 1 86 1 0.0 6 0 0 0 0 0 0 1 167 1 0.0 6 1 195 1 0.0 6 1 228 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 61294 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 958.8 0 0 2 5 1 59.9 1 1 6 2 15.0 1 2 9 1 0.2 2 1 10 3 0.1 3 3 11 1 0.0 3 1 12 2 0.0 3 2 13 10 0.0 3 8 2 14 7 0.0 4 6 0 1 16 15 0.0 4 11 4 17 23 0.0 5 18 4 1 18 9 0.0 5 8 1 19 17 0.0 5 13 4 20 64 0.0 6 60 4 21 52 0.0 6 49 3 22 28 0.0 6 28 23 41 0.0 6 37 4 24 30 0.0 6 30 25 33 0.0 6 31 1 1 26 21 0.0 6 19 1 0 0 1 27 9 0.0 6 9 28 18 0.0 6 16 2 29 25 0.0 6 23 1 1 30 26 0.0 6 24 2 31 11 0.0 6 11 32 13 0.0 6 13 33 4 0.0 6 4 34 10 0.0 6 10 35 12 0.0 6 10 2 36 7 0.0 6 5 2 37 11 0.0 6 10 1 38 4 0.0 6 4 39 7 0.0 6 7 40 10 0.0 6 8 2 41 16 0.0 6 14 2 42 7 0.0 6 5 2 43 12 0.0 6 9 1 2 44 20 0.0 6 19 1 45 5 0.0 6 3 2 46 26 0.0 6 24 1 1 47 18 0.0 6 16 2 48 21 0.0 6 19 2 49 23 0.0 6 20 2 1 50 14 0.0 6 12 2 51 12 0.0 6 11 1 52 30 0.0 6 28 2 53 21 0.0 6 18 3 54 26 0.0 6 23 2 0 0 0 1 55 19 0.0 6 16 3 56 16 0.0 6 15 1 57 27 0.0 6 25 1 0 0 1 58 16 0.0 6 13 3 59 15 0.0 6 13 2 60 20 0.0 6 18 2 61 9 0.0 6 9 62 17 0.0 6 16 1 63 7 0.0 6 5 2 64 11 0.0 6 9 1 0 0 1 65 10 0.0 6 8 2 66 28 0.0 6 25 1 1 0 1 67 133 0.0 6 131 1 0 0 1 68 28 0.0 6 27 1 69 41 0.0 6 40 1 70 15 0.0 6 12 3 71 23 0.0 6 21 2 72 27 0.0 6 26 1 73 13 0.0 6 11 2 74 11 0.0 6 10 0 1 75 15 0.0 6 13 1 1 76 20 0.0 6 18 1 1 77 13 0.0 6 11 2 78 36 0.0 6 32 2 2 79 28 0.0 6 27 1 80 13 0.0 6 12 1 81 13 0.0 6 12 0 1 82 33 0.0 6 29 1 2 0 1 83 58 0.0 6 53 5 84 34 0.0 6 31 3 85 27 0.0 6 23 3 1 86 19 0.0 6 18 1 87 44 0.0 6 37 7 88 21 0.0 6 18 3 89 26 0.0 6 26 90 38 0.0 6 33 3 0 1 1 91 27 0.0 6 23 4 92 27 0.0 6 26 1 93 47 0.0 6 41 4 0 1 0 1 94 82 0.0 6 72 10 95 30 0.0 6 26 4 96 44 0.0 6 38 5 1 97 43 0.0 6 40 3 98 39 0.0 6 36 3 99 39 0.0 6 36 2 1 100 32 0.0 6 30 2 101 35 0.0 6 31 3 1 102 79 0.0 6 76 2 1 103 72 0.0 6 65 6 1 104 64 0.0 6 60 2 2 105 56 0.0 6 51 4 1 106 49 0.0 6 45 4 107 53 0.0 6 47 5 1 108 49 0.0 6 46 3 109 43 0.0 6 38 4 0 1 110 44 0.0 6 42 1 1 111 43 0.0 6 40 3 112 40 0.0 6 35 5 113 33 0.0 6 32 1 114 69 0.0 6 65 4 115 42 0.0 6 40 1 0 0 1 116 63 0.0 6 55 8 117 35 0.0 6 33 2 118 54 0.0 6 52 1 1 119 40 0.0 6 34 4 1 0 1 120 127 0.0 6 118 8 1 121 68 0.0 6 64 3 0 0 1 122 106 0.0 6 104 2 123 87 0.0 6 82 5 124 120 0.0 6 113 6 1 125 99 0.0 6 94 5 126 95 0.0 6 89 6 127 116 0.0 6 113 3 128 60 0.0 6 58 2 129 60 0.0 6 56 2 2 130 71 0.0 6 68 3 131 167 0.0 6 162 4 1 132 220 0.0 6 207 12 1 133 217 0.0 6 206 10 1 134 276 0.0 6 265 10 0 0 1 135 412 0.0 6 398 13 0 0 1 136 110 0.0 6 104 6 137 199 0.0 6 189 8 2 138 96 0.0 6 90 6 139 223 0.0 6 214 9 140 300 0.0 6 283 16 0 1 141 264 0.0 6 253 10 0 1 142 450 0.0 6 430 15 2 0 3 143 343 0.0 6 325 16 1 1 144 487 0.0 6 475 11 1 145 214 0.0 6 206 8 146 213 0.0 6 202 10 0 0 1 147 136 0.0 6 129 6 1 148 237 0.0 6 231 5 1 149 462 0.0 6 446 15 0 0 1 150 728 0.0 6 702 26 151 193 0.0 6 174 17 0 0 2 152 682 0.0 6 659 21 0 1 1 153 341 0.0 6 324 15 1 1 154 510 0.0 6 499 10 1 155 440 0.0 6 423 17 156 389 0.0 6 374 14 1 157 565 0.0 6 547 17 0 0 1 158 560 0.0 6 542 18 159 256 0.0 6 244 11 0 1 160 473 0.0 6 460 13 161 310 0.0 6 292 18 162 202 0.0 6 195 6 0 1 163 368 0.0 6 356 11 1 164 392 0.0 6 381 11 165 441 0.0 6 415 23 2 0 1 166 594 0.0 6 581 12 0 0 1 167 249 0.0 6 237 12 168 928 0.0 6 906 21 0 0 1 169 208 0.0 6 197 8 1 2 170 354 0.0 6 340 13 1 171 924 0.0 6 903 21 172 247 0.0 6 245 2 173 315 0.0 6 305 7 3 174 546 0.0 6 520 24 1 1 175 987 0.0 6 959 28 176 570 0.0 6 551 17 2 177 598 0.0 6 576 20 1 0 1 178 513 0.0 6 493 20 179 686 0.0 6 664 21 0 0 1 180 558 0.0 6 537 21 181 810 0.0 6 789 19 1 0 1 182 160 0.0 6 142 16 1 0 1 183 603 0.0 6 582 18 1 0 2 184 706 0.0 6 688 16 1 1 185 831 0.0 6 803 27 1 186 1391 0.0 6 1349 41 1 187 1360 0.0 6 1322 37 0 0 1 188 1243 0.0 6 1203 36 1 1 1 1 189 1112 0.0 6 1074 37 0 0 0 0 1 190 1553 0.0 6 1504 44 2 0 3 191 1540 0.0 6 1496 42 2 192 943 0.0 6 925 18 193 564 0.0 6 540 24 194 1187 0.0 6 1158 27 2 195 1682 0.0 6 1618 61 2 0 1 196 2930 0.0 6 2864 60 2 1 3 197 773 0.0 6 746 25 1 0 0 1 198 1636 0.0 6 1579 56 0 0 1 199 2861 0.0 6 2786 68 2 0 4 1 200 854 0.0 6 835 19 201 1225 0.0 6 1200 25 202 559 0.0 6 549 8 2 203 595 0.0 6 576 18 0 0 1 204 722 0.0 6 700 20 1 1 205 286 0.0 6 278 7 0 1 206 261 0.0 6 255 6 207 157 0.0 6 151 6 208 350 0.0 6 341 8 0 1 209 259 0.0 6 254 4 0 1 210 253 0.0 6 249 4 211 187 0.0 6 184 3 212 345 0.0 6 335 10 213 145 0.0 6 141 3 1 214 507 0.0 6 492 14 1 215 431 0.0 6 422 8 0 1 216 675 0.0 6 652 23 217 254 0.0 6 247 7 218 244 0.0 6 238 6 219 225 0.0 6 223 2 220 53 0.0 6 53 221 245 0.0 6 240 4 1 222 478 0.0 6 468 8 0 2 223 683 0.0 6 668 14 0 0 1 224 160 0.0 6 155 5 225 370 0.0 6 362 8 226 125 0.0 6 123 2 227 253 0.0 6 249 4 228 94 0.0 6 89 4 1 229 290 0.0 6 287 3 230 122 0.0 6 120 2 231 212 0.0 6 210 2 232 307 0.0 6 297 10 233 184 0.0 6 183 1 234 87 0.0 6 86 1 235 40 0.0 6 40 236 8 0.0 6 7 1 237 22 0.0 6 22 238 24 0.0 6 23 1 239 21 0.0 6 21 240 77 0.0 6 76 1 241 126 0.0 6 121 5 242 170 0.0 6 165 5 243 145 0.0 6 144 1 244 92 0.0 6 88 4 245 37 0.0 6 36 1 246 39 0.0 6 38 0 1 247 14 0.0 6 14 248 40 0.0 6 39 1 249 8 0.0 6 8 250 3 0.0 6 3 251 23 0.0 6 22 1 252 3 0.0 6 3 253 5 0.0 6 5 276 1 0.0 6 1 277 3 0.0 6 3 278 50 0.0 6 49 1 279 20 0.0 6 19 1 280 1 0.0 6 1 281 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 21 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 13 2 0.0 3 0 0 0 0 2 72 4 0.0 6 4 73 1 0.0 6 1 74 1 0.0 6 1 78 2 0.0 6 2 89 1 0.0 6 1 90 1 0.0 6 1 95 1 0.0 6 1 97 1 0.0 6 1 111 1 0.0 6 1 112 1 0.0 6 1 136 1 0.0 6 1 144 1 0.0 6 1 146 1 0.0 6 1 155 1 0.0 6 1 197 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_01_84_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_01_311_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_01_84_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_01_311_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_02_85_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_02_312_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_02_85_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_02_312_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_03_86_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_03_313_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_03_86_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_03_313_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_04_87_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_04_314_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_04_87_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_04_314_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_05_88_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_05_315_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_05_88_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_05_315_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_06_89_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_06_316_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_06_89_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_06_316_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_15_90_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_15_317_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_15_90_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_15_317_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_16_91_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_16_318_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_16_91_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_16_318_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_17_92_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_17_319_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_17_92_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_17_319_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_18_93_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_18_320_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_18_93_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_18_320_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_19_94_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_19_321_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_19_94_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_19_321_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_20_95_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_08-Rev_20_322_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_20_95_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_08-Rev_20_322_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_01_96_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_01_323_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_01_96_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_01_323_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.03 s (31 µs/read; 1.93 M reads/minute). === Summary === Total read pairs processed: 97,633 Read 1 with adapter: 97,627 (100.0%) Read 2 with adapter: 97,569 (99.9%) == Read fate breakdown == Pairs that were too short: 10 (0.0%) Pairs discarded as untrimmed: 70 (0.1%) Pairs written (passing filters): 97,553 (99.9%) Total basepairs processed: 47,924,564 bp Read 1: 27,337,315 bp Read 2: 20,587,249 bp Total written (filtered): 5,972,124 bp (12.5%) Read 1: 3,039,155 bp Read 2: 2,932,969 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 97257 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 381.4 1 0 1 6 3 23.8 1 1 0 2 7 2 6.0 2 1 0 1 9 2 0.4 2 2 16 2 0.0 4 1 1 17 1 0.0 5 1 18 1 0.0 5 0 1 19 2 0.0 5 2 20 47 0.0 6 46 1 21 26 0.0 6 26 22 22 0.0 6 22 23 8 0.0 6 7 1 24 8 0.0 6 8 25 25 0.0 6 25 26 10 0.0 6 9 1 27 3 0.0 6 3 28 10 0.0 6 9 1 29 3 0.0 6 3 30 1 0.0 6 1 31 2 0.0 6 2 33 6 0.0 6 6 34 3 0.0 6 3 35 2 0.0 6 2 36 1 0.0 6 1 37 4 0.0 6 4 38 8 0.0 6 7 0 0 0 1 39 3 0.0 6 3 40 7 0.0 6 7 42 1 0.0 6 1 43 3 0.0 6 3 44 4 0.0 6 3 1 45 6 0.0 6 5 1 46 5 0.0 6 5 47 2 0.0 6 2 49 8 0.0 6 7 1 50 9 0.0 6 8 1 51 1 0.0 6 1 52 6 0.0 6 5 1 53 2 0.0 6 2 54 6 0.0 6 6 55 3 0.0 6 3 56 4 0.0 6 4 57 5 0.0 6 5 59 2 0.0 6 2 62 6 0.0 6 5 1 63 6 0.0 6 6 64 4 0.0 6 4 66 5 0.0 6 3 2 67 5 0.0 6 4 1 68 3 0.0 6 3 69 8 0.0 6 8 70 4 0.0 6 3 1 71 5 0.0 6 5 72 1 0.0 6 1 74 2 0.0 6 2 75 1 0.0 6 1 76 5 0.0 6 4 1 77 6 0.0 6 6 78 4 0.0 6 4 79 4 0.0 6 4 80 1 0.0 6 1 81 3 0.0 6 3 82 4 0.0 6 4 83 3 0.0 6 3 84 5 0.0 6 5 85 2 0.0 6 2 86 9 0.0 6 9 87 10 0.0 6 10 88 7 0.0 6 5 1 1 89 9 0.0 6 9 90 8 0.0 6 7 1 91 5 0.0 6 5 92 7 0.0 6 5 2 93 8 0.0 6 6 2 94 1 0.0 6 1 95 3 0.0 6 3 96 1 0.0 6 1 97 8 0.0 6 7 1 98 9 0.0 6 9 99 8 0.0 6 8 100 7 0.0 6 7 101 13 0.0 6 12 1 102 9 0.0 6 9 103 9 0.0 6 9 104 8 0.0 6 7 1 105 4 0.0 6 4 106 6 0.0 6 5 1 107 10 0.0 6 10 108 22 0.0 6 20 1 1 109 8 0.0 6 8 110 7 0.0 6 4 3 111 11 0.0 6 10 1 112 9 0.0 6 8 1 113 7 0.0 6 5 2 114 12 0.0 6 11 1 115 10 0.0 6 9 1 116 8 0.0 6 5 2 1 117 13 0.0 6 12 1 118 16 0.0 6 16 119 9 0.0 6 9 120 4 0.0 6 4 121 13 0.0 6 11 2 122 9 0.0 6 9 123 23 0.0 6 22 1 124 11 0.0 6 11 125 14 0.0 6 12 2 126 8 0.0 6 8 127 16 0.0 6 16 128 21 0.0 6 21 129 22 0.0 6 22 130 23 0.0 6 20 3 131 8 0.0 6 8 132 8 0.0 6 8 133 19 0.0 6 19 134 19 0.0 6 19 135 33 0.0 6 32 1 136 20 0.0 6 20 137 53 0.0 6 52 1 138 27 0.0 6 27 139 30 0.0 6 28 2 140 46 0.0 6 45 1 141 97 0.0 6 96 1 142 176 0.0 6 174 1 1 143 129 0.0 6 126 3 144 94 0.0 6 93 1 145 87 0.0 6 86 1 146 142 0.0 6 139 3 147 130 0.0 6 128 2 148 105 0.0 6 103 2 149 123 0.0 6 121 2 150 132 0.0 6 131 1 151 86 0.0 6 84 2 152 78 0.0 6 78 153 89 0.0 6 88 0 1 154 69 0.0 6 69 155 70 0.0 6 70 156 67 0.0 6 66 1 157 73 0.0 6 71 2 158 72 0.0 6 68 4 159 85 0.0 6 83 1 0 0 0 1 160 74 0.0 6 72 2 161 147 0.0 6 145 2 162 89 0.0 6 89 163 77 0.0 6 76 0 0 1 164 119 0.0 6 118 1 165 81 0.0 6 79 2 166 52 0.0 6 52 167 61 0.0 6 59 2 168 87 0.0 6 85 0 1 0 1 169 109 0.0 6 108 1 170 124 0.0 6 124 171 89 0.0 6 88 1 172 134 0.0 6 129 5 173 110 0.0 6 110 174 131 0.0 6 128 1 1 0 0 0 1 175 110 0.0 6 107 2 0 0 0 0 1 176 71 0.0 6 69 2 177 101 0.0 6 101 178 102 0.0 6 100 1 1 179 164 0.0 6 163 1 180 139 0.0 6 136 3 181 145 0.0 6 141 4 182 158 0.0 6 153 5 183 111 0.0 6 108 3 184 119 0.0 6 113 6 185 214 0.0 6 208 5 1 186 278 0.0 6 269 9 187 265 0.0 6 261 4 188 269 0.0 6 260 8 0 1 189 184 0.0 6 176 8 190 205 0.0 6 201 4 191 235 0.0 6 229 6 192 264 0.0 6 254 10 193 360 0.0 6 348 11 1 194 320 0.0 6 311 9 195 392 0.0 6 386 6 196 435 0.0 6 421 14 197 454 0.0 6 436 18 198 550 0.0 6 536 13 1 199 461 0.0 6 446 14 0 0 0 1 200 545 0.0 6 526 19 201 485 0.0 6 472 13 202 508 0.0 6 496 12 203 403 0.0 6 392 9 2 204 485 0.0 6 471 12 1 0 0 0 1 205 514 0.0 6 500 13 0 1 206 595 0.0 6 583 11 0 1 207 615 0.0 6 604 9 1 1 208 472 0.0 6 462 10 209 434 0.0 6 423 11 210 315 0.0 6 306 8 0 0 0 1 211 353 0.0 6 345 7 0 1 212 425 0.0 6 418 6 1 213 460 0.0 6 451 9 214 675 0.0 6 660 15 215 488 0.0 6 473 15 216 549 0.0 6 531 17 0 0 0 0 1 217 423 0.0 6 418 5 218 445 0.0 6 436 9 219 509 0.0 6 503 6 220 397 0.0 6 390 7 221 294 0.0 6 289 5 222 386 0.0 6 368 15 2 1 223 840 0.0 6 819 20 1 224 689 0.0 6 666 23 225 704 0.0 6 684 17 2 1 226 683 0.0 6 669 14 227 680 0.0 6 672 8 228 441 0.0 6 437 4 229 413 0.0 6 408 5 230 390 0.0 6 382 8 231 427 0.0 6 423 4 232 425 0.0 6 421 4 233 382 0.0 6 379 2 0 1 234 407 0.0 6 396 11 235 325 0.0 6 318 7 236 309 0.0 6 295 13 0 0 1 237 577 0.0 6 565 11 1 238 901 0.0 6 875 25 0 0 0 0 1 239 946 0.0 6 925 20 1 240 797 0.0 6 770 27 241 647 0.0 6 628 16 3 242 1005 0.0 6 983 21 1 243 580 0.0 6 567 12 0 1 244 622 0.0 6 603 18 1 245 470 0.0 6 460 10 246 727 0.0 6 714 12 0 1 247 489 0.0 6 477 11 0 1 248 625 0.0 6 610 15 249 642 0.0 6 632 9 0 1 250 521 0.0 6 508 12 0 1 251 763 0.0 6 745 17 1 252 648 0.0 6 630 17 1 253 422 0.0 6 409 12 1 254 429 0.0 6 419 9 0 0 1 255 386 0.0 6 373 12 0 1 256 741 0.0 6 716 25 257 860 0.0 6 840 20 258 573 0.0 6 555 16 2 259 733 0.0 6 713 19 0 1 260 1275 0.0 6 1244 29 2 261 952 0.0 6 931 20 1 262 563 0.0 6 553 9 1 263 367 0.0 6 357 10 264 548 0.0 6 534 13 0 0 0 0 1 265 504 0.0 6 492 12 266 902 0.0 6 871 28 2 0 1 267 500 0.0 6 490 8 2 268 460 0.0 6 436 22 1 1 269 2121 0.0 6 2068 50 3 270 1384 0.0 6 1353 30 0 0 0 0 1 271 868 0.0 6 848 18 1 1 272 507 0.0 6 491 16 273 921 0.0 6 887 33 1 274 2550 0.0 6 2489 59 2 275 1778 0.0 6 1741 37 276 1454 0.0 6 1413 38 3 277 2224 0.0 6 2158 59 5 2 278 2215 0.0 6 2073 131 10 1 279 30053 0.0 6 29259 782 12 280 1450 0.0 6 1323 117 7 2 1 281 72 0.0 6 63 7 0 2 282 6 0.0 6 6 283 1 0.0 6 1 284 4 0.0 6 4 285 1 0.0 6 1 286 2 0.0 6 2 290 1 0.0 6 1 291 1 0.0 6 0 0 0 0 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 370 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 1 0.0 6 1 71 2 0.0 6 2 72 6 0.0 6 6 73 3 0.0 6 3 74 4 0.0 6 4 75 3 0.0 6 3 77 1 0.0 6 1 78 4 0.0 6 4 79 2 0.0 6 2 80 1 0.0 6 1 81 4 0.0 6 4 82 1 0.0 6 1 83 1 0.0 6 1 84 3 0.0 6 3 85 1 0.0 6 1 86 2 0.0 6 2 87 1 0.0 6 1 88 4 0.0 6 4 89 1 0.0 6 1 91 6 0.0 6 6 92 4 0.0 6 4 93 1 0.0 6 1 95 3 0.0 6 3 96 1 0.0 6 1 97 2 0.0 6 2 98 1 0.0 6 1 99 1 0.0 6 1 100 3 0.0 6 3 104 3 0.0 6 3 105 1 0.0 6 1 106 1 0.0 6 1 107 1 0.0 6 1 108 2 0.0 6 2 109 4 0.0 6 4 110 1 0.0 6 1 113 1 0.0 6 1 115 4 0.0 6 4 116 3 0.0 6 3 118 1 0.0 6 1 119 1 0.0 6 1 120 2 0.0 6 2 122 1 0.0 6 1 123 2 0.0 6 2 124 3 0.0 6 3 126 1 0.0 6 1 127 1 0.0 6 1 131 1 0.0 6 1 132 2 0.0 6 2 133 1 0.0 6 1 134 3 0.0 6 3 135 6 0.0 6 6 136 1 0.0 6 1 137 1 0.0 6 1 138 2 0.0 6 2 139 1 0.0 6 1 141 2 0.0 6 2 142 2 0.0 6 2 143 1 0.0 6 1 144 2 0.0 6 2 145 1 0.0 6 1 147 1 0.0 6 1 148 1 0.0 6 1 149 2 0.0 6 2 150 1 0.0 6 1 151 2 0.0 6 2 152 2 0.0 6 2 153 3 0.0 6 3 154 5 0.0 6 5 156 1 0.0 6 1 159 1 0.0 6 1 160 2 0.0 6 2 161 2 0.0 6 2 162 4 0.0 6 4 163 6 0.0 6 6 164 1 0.0 6 1 165 1 0.0 6 1 167 2 0.0 6 2 168 6 0.0 6 6 169 5 0.0 6 5 170 1 0.0 6 1 171 2 0.0 6 2 172 1 0.0 6 1 173 1 0.0 6 1 174 2 0.0 6 2 175 2 0.0 6 2 176 2 0.0 6 2 177 4 0.0 6 4 178 1 0.0 6 1 179 2 0.0 6 2 180 1 0.0 6 1 182 2 0.0 6 2 184 2 0.0 6 2 186 2 0.0 6 2 187 1 0.0 6 1 188 1 0.0 6 1 189 4 0.0 6 4 190 4 0.0 6 4 191 1 0.0 6 1 192 3 0.0 6 3 193 4 0.0 6 4 194 3 0.0 6 3 195 2 0.0 6 2 197 4 0.0 6 4 198 2 0.0 6 2 199 4 0.0 6 4 200 6 0.0 6 6 201 1 0.0 6 1 202 1 0.0 6 1 203 2 0.0 6 2 205 4 0.0 6 4 206 4 0.0 6 4 207 1 0.0 6 1 208 4 0.0 6 4 209 2 0.0 6 2 210 2 0.0 6 2 211 2 0.0 6 2 212 1 0.0 6 1 213 5 0.0 6 5 214 3 0.0 6 3 215 1 0.0 6 1 218 1 0.0 6 1 219 2 0.0 6 2 220 4 0.0 6 4 221 4 0.0 6 4 223 4 0.0 6 4 224 4 0.0 6 4 226 2 0.0 6 2 227 1 0.0 6 1 228 2 0.0 6 2 230 1 0.0 6 1 231 5 0.0 6 5 232 1 0.0 6 1 233 2 0.0 6 2 234 3 0.0 6 3 235 6 0.0 6 6 236 3 0.0 6 3 237 3 0.0 6 3 238 2 0.0 6 2 239 3 0.0 6 3 241 1 0.0 6 1 242 1 0.0 6 1 244 2 0.0 6 2 245 1 0.0 6 1 246 3 0.0 6 3 247 3 0.0 6 3 248 3 0.0 6 3 249 2 0.0 6 2 250 2 0.0 6 2 252 2 0.0 6 2 253 3 0.0 6 3 254 2 0.0 6 2 255 1 0.0 6 1 256 2 0.0 6 2 257 1 0.0 6 1 258 2 0.0 6 2 262 1 0.0 6 1 263 1 0.0 6 1 266 1 0.0 6 1 267 1 0.0 6 1 268 1 0.0 6 1 269 1 0.0 6 1 270 1 0.0 6 1 274 1 0.0 6 1 276 1 0.0 6 1 279 1 0.0 6 1 288 1 0.0 6 1 295 1 0.0 6 1 298 1 0.0 6 1 302 1 0.0 6 1 303 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 97318 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 9 2 0.4 2 1 1 10 1 0.1 3 1 11 4 0.0 3 4 12 5 0.0 3 4 1 13 18 0.0 3 16 2 14 6 0.0 4 5 1 15 2 0.0 4 2 16 16 0.0 4 14 2 17 22 0.0 5 20 2 18 17 0.0 5 13 4 19 20 0.0 5 17 2 1 20 92 0.0 6 88 4 21 84 0.0 6 77 6 0 1 22 55 0.0 6 51 3 0 1 23 41 0.0 6 37 3 1 24 20 0.0 6 20 25 16 0.0 6 16 26 27 0.0 6 27 27 18 0.0 6 16 2 28 14 0.0 6 13 1 29 21 0.0 6 21 30 20 0.0 6 18 1 1 31 8 0.0 6 8 32 13 0.0 6 13 33 14 0.0 6 12 2 34 17 0.0 6 16 1 35 14 0.0 6 14 36 11 0.0 6 11 37 15 0.0 6 15 38 29 0.0 6 27 2 39 16 0.0 6 15 1 40 12 0.0 6 12 41 12 0.0 6 11 1 42 13 0.0 6 13 43 8 0.0 6 8 44 23 0.0 6 22 1 45 14 0.0 6 13 1 46 24 0.0 6 23 0 0 0 1 47 31 0.0 6 28 3 48 12 0.0 6 12 49 29 0.0 6 28 1 50 37 0.0 6 34 3 51 8 0.0 6 5 3 52 27 0.0 6 26 1 53 17 0.0 6 17 54 28 0.0 6 23 4 1 55 27 0.0 6 24 3 56 35 0.0 6 31 4 57 23 0.0 6 21 2 58 23 0.0 6 22 1 59 19 0.0 6 18 1 60 29 0.0 6 26 2 0 0 1 61 17 0.0 6 13 4 62 30 0.0 6 29 1 63 15 0.0 6 15 64 21 0.0 6 20 1 65 26 0.0 6 23 3 66 39 0.0 6 33 6 67 36 0.0 6 32 2 1 0 1 68 52 0.0 6 47 5 69 184 0.0 6 176 7 1 70 52 0.0 6 50 2 71 83 0.0 6 79 3 1 72 75 0.0 6 71 4 73 55 0.0 6 53 2 74 33 0.0 6 32 1 75 27 0.0 6 27 76 45 0.0 6 35 10 77 43 0.0 6 38 5 78 40 0.0 6 39 1 79 41 0.0 6 38 3 80 36 0.0 6 33 3 81 26 0.0 6 24 1 0 1 82 71 0.0 6 66 4 1 83 65 0.0 6 61 3 1 84 56 0.0 6 54 2 85 72 0.0 6 65 7 86 46 0.0 6 43 3 87 61 0.0 6 59 2 88 28 0.0 6 27 0 0 0 0 0 1 89 65 0.0 6 61 3 1 90 58 0.0 6 54 4 91 55 0.0 6 53 1 0 0 1 92 42 0.0 6 41 1 93 105 0.0 6 96 9 94 144 0.0 6 135 9 95 69 0.0 6 66 2 0 0 0 0 1 96 68 0.0 6 65 1 1 1 97 64 0.0 6 59 4 0 0 0 1 98 84 0.0 6 79 5 99 77 0.0 6 74 2 0 0 0 0 1 100 75 0.0 6 72 2 1 101 70 0.0 6 66 3 0 0 1 102 111 0.0 6 107 4 103 107 0.0 6 100 6 1 104 100 0.0 6 87 10 2 1 105 125 0.0 6 121 4 106 113 0.0 6 103 10 107 108 0.0 6 100 7 1 108 80 0.0 6 74 5 0 0 1 109 90 0.0 6 88 1 1 110 76 0.0 6 72 4 111 108 0.0 6 103 4 1 112 104 0.0 6 100 3 1 113 85 0.0 6 82 2 1 114 108 0.0 6 107 0 0 0 1 115 116 0.0 6 108 7 1 116 102 0.0 6 97 4 1 117 89 0.0 6 86 1 0 2 118 124 0.0 6 118 6 119 165 0.0 6 158 6 1 120 161 0.0 6 151 7 2 0 1 121 104 0.0 6 97 6 0 0 1 122 138 0.0 6 135 2 0 0 1 123 112 0.0 6 111 1 124 145 0.0 6 141 4 125 110 0.0 6 106 4 126 106 0.0 6 104 2 127 154 0.0 6 147 4 2 0 1 128 111 0.0 6 109 1 0 0 1 129 154 0.0 6 151 2 1 130 145 0.0 6 140 3 1 0 0 0 1 131 239 0.0 6 230 9 132 233 0.0 6 229 4 133 184 0.0 6 176 5 3 134 234 0.0 6 226 8 135 512 0.0 6 488 19 0 0 4 1 136 295 0.0 6 281 12 2 137 354 0.0 6 343 10 0 0 0 1 138 212 0.0 6 201 8 1 1 0 0 1 139 348 0.0 6 341 7 140 495 0.0 6 479 13 1 2 141 457 0.0 6 443 12 0 1 0 0 1 142 596 0.0 6 582 14 143 531 0.0 6 514 14 1 0 1 0 1 144 687 0.0 6 667 19 0 1 145 497 0.0 6 488 8 0 1 146 505 0.0 6 483 19 2 0 1 147 348 0.0 6 337 9 0 0 2 148 470 0.0 6 455 11 2 1 1 149 643 0.0 6 622 18 2 0 1 150 828 0.0 6 800 28 151 512 0.0 6 495 17 152 1221 0.0 6 1183 33 3 1 1 153 581 0.0 6 560 17 2 0 1 0 1 154 877 0.0 6 853 24 155 867 0.0 6 839 23 2 1 0 1 1 156 636 0.0 6 618 14 1 0 3 157 873 0.0 6 845 25 2 0 1 158 865 0.0 6 844 18 1 2 159 574 0.0 6 546 26 2 160 852 0.0 6 823 25 1 1 1 0 1 161 550 0.0 6 536 10 2 2 162 577 0.0 6 556 19 1 1 163 685 0.0 6 666 16 1 0 0 0 2 164 588 0.0 6 573 14 0 0 1 165 738 0.0 6 711 25 0 0 1 0 1 166 718 0.0 6 701 16 0 0 0 0 1 167 467 0.0 6 451 14 0 1 1 168 835 0.0 6 810 24 0 1 169 418 0.0 6 401 15 0 1 0 1 170 536 0.0 6 520 16 171 689 0.0 6 667 20 1 0 0 1 172 501 0.0 6 485 13 2 0 1 173 737 0.0 6 716 18 1 1 1 174 1014 0.0 6 976 34 3 0 1 175 1793 0.0 6 1743 49 0 1 176 1071 0.0 6 1047 21 2 1 177 970 0.0 6 930 37 1 0 1 0 1 178 840 0.0 6 812 25 2 0 1 179 905 0.0 6 883 22 180 867 0.0 6 836 30 0 0 1 181 909 0.0 6 885 21 2 1 182 452 0.0 6 424 26 2 183 1001 0.0 6 967 31 2 0 0 1 184 227 0.0 6 216 9 2 185 596 0.0 6 576 19 0 0 1 186 1937 0.0 6 1890 45 0 0 1 0 1 187 1767 0.0 6 1701 61 1 1 3 188 1890 0.0 6 1824 60 4 0 2 189 1488 0.0 6 1436 47 3 1 0 0 1 190 2529 0.0 6 2459 67 2 0 1 191 1956 0.0 6 1897 58 1 192 1290 0.0 6 1250 39 0 0 0 0 1 193 1008 0.0 6 965 43 194 1625 0.0 6 1570 53 1 0 1 195 3070 0.0 6 2972 91 2 2 3 196 2868 0.0 6 2777 87 1 0 3 197 742 0.0 6 712 24 3 1 2 198 3090 0.0 6 2970 116 0 0 4 199 4584 0.0 6 4475 103 1 1 2 2 200 1366 0.0 6 1328 36 0 1 1 201 1685 0.0 6 1616 67 2 202 1042 0.0 6 1003 39 203 976 0.0 6 943 30 2 0 1 204 1617 0.0 6 1577 39 1 205 418 0.0 6 403 14 0 1 206 410 0.0 6 397 13 207 268 0.0 6 257 8 2 1 208 564 0.0 6 541 23 209 481 0.0 6 473 8 210 391 0.0 6 375 15 0 0 0 0 1 211 483 0.0 6 472 11 212 428 0.0 6 417 11 213 154 0.0 6 147 6 1 214 479 0.0 6 455 22 0 1 1 215 759 0.0 6 727 32 216 881 0.0 6 847 32 1 0 1 217 987 0.0 6 963 23 1 218 797 0.0 6 775 19 2 0 1 219 859 0.0 6 826 29 1 0 3 220 484 0.0 6 455 25 1 1 2 221 758 0.0 6 733 25 222 154 0.0 6 142 10 0 2 223 687 0.0 6 664 22 1 224 889 0.0 6 861 27 1 225 1000 0.0 6 969 30 1 226 968 0.0 6 938 30 227 533 0.0 6 514 19 228 350 0.0 6 335 14 0 0 1 229 488 0.0 6 469 18 0 0 1 230 378 0.0 6 363 15 231 381 0.0 6 376 5 232 362 0.0 6 353 7 1 0 1 233 602 0.0 6 585 17 234 376 0.0 6 365 11 235 137 0.0 6 130 7 236 91 0.0 6 87 4 237 76 0.0 6 75 1 238 40 0.0 6 38 2 239 30 0.0 6 29 1 240 164 0.0 6 159 5 241 151 0.0 6 148 3 242 260 0.0 6 254 6 243 290 0.0 6 283 7 244 173 0.0 6 169 4 245 94 0.0 6 89 5 246 105 0.0 6 102 3 247 65 0.0 6 63 2 248 64 0.0 6 60 4 249 80 0.0 6 79 1 250 38 0.0 6 38 251 16 0.0 6 15 1 252 10 0.0 6 10 253 29 0.0 6 26 3 254 9 0.0 6 9 255 5 0.0 6 5 257 1 0.0 6 1 258 1 0.0 6 1 260 1 0.0 6 1 261 1 0.0 6 1 279 4 0.0 6 4 280 16 0.0 6 16 281 46 0.0 6 43 2 0 0 0 0 1 282 1 0.0 6 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 251 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 50 1 0.0 6 1 51 1 0.0 6 0 1 53 1 0.0 6 1 62 1 0.0 6 1 69 2 0.0 6 2 70 1 0.0 6 1 71 3 0.0 6 3 72 4 0.0 6 4 73 2 0.0 6 2 74 4 0.0 6 4 75 6 0.0 6 6 76 3 0.0 6 3 77 1 0.0 6 1 78 4 0.0 6 4 79 9 0.0 6 9 80 4 0.0 6 4 81 3 0.0 6 3 82 3 0.0 6 3 83 1 0.0 6 1 84 1 0.0 6 1 85 2 0.0 6 2 86 2 0.0 6 2 87 1 0.0 6 1 88 1 0.0 6 1 89 1 0.0 6 1 90 1 0.0 6 1 91 2 0.0 6 2 92 2 0.0 6 2 93 2 0.0 6 2 94 3 0.0 6 3 95 1 0.0 6 1 96 3 0.0 6 3 97 1 0.0 6 1 99 2 0.0 6 2 100 1 0.0 6 1 101 1 0.0 6 1 102 2 0.0 6 2 104 3 0.0 6 3 105 1 0.0 6 1 106 1 0.0 6 1 107 1 0.0 6 1 108 4 0.0 6 4 109 2 0.0 6 2 110 1 0.0 6 1 112 2 0.0 6 2 113 1 0.0 6 1 114 1 0.0 6 1 115 3 0.0 6 3 117 2 0.0 6 2 118 1 0.0 6 1 120 1 0.0 6 1 121 2 0.0 6 2 122 4 0.0 6 4 123 2 0.0 6 2 124 4 0.0 6 4 125 2 0.0 6 2 126 1 0.0 6 1 127 2 0.0 6 2 129 1 0.0 6 1 132 1 0.0 6 1 133 3 0.0 6 3 136 2 0.0 6 2 137 2 0.0 6 2 138 6 0.0 6 6 140 2 0.0 6 2 141 1 0.0 6 1 142 3 0.0 6 3 143 3 0.0 6 3 144 1 0.0 6 1 145 2 0.0 6 2 146 1 0.0 6 1 148 3 0.0 6 3 150 3 0.0 6 3 152 1 0.0 6 1 153 1 0.0 6 1 154 1 0.0 6 1 155 4 0.0 6 4 156 2 0.0 6 2 157 3 0.0 6 3 159 1 0.0 6 1 160 3 0.0 6 3 161 1 0.0 6 1 162 1 0.0 6 1 163 3 0.0 6 3 165 1 0.0 6 1 166 1 0.0 6 1 167 2 0.0 6 2 168 2 0.0 6 2 169 1 0.0 6 1 170 2 0.0 6 2 171 2 0.0 6 2 172 3 0.0 6 3 173 2 0.0 6 2 175 2 0.0 6 2 176 2 0.0 6 2 177 1 0.0 6 1 178 5 0.0 6 5 179 1 0.0 6 1 184 1 0.0 6 1 185 1 0.0 6 1 187 1 0.0 6 1 188 2 0.0 6 2 189 3 0.0 6 3 190 2 0.0 6 2 191 2 0.0 6 2 192 5 0.0 6 5 193 2 0.0 6 2 195 3 0.0 6 2 0 0 0 1 196 3 0.0 6 3 197 1 0.0 6 1 199 3 0.0 6 3 200 1 0.0 6 1 202 1 0.0 6 1 204 1 0.0 6 1 205 1 0.0 6 1 206 1 0.0 6 1 208 2 0.0 6 2 210 1 0.0 6 1 213 1 0.0 6 1 214 1 0.0 6 1 217 2 0.0 6 2 220 1 0.0 6 1 223 1 0.0 6 1 235 1 0.0 6 1 244 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_02_97_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_02_324_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_02_97_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_02_324_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.76 s (44 µs/read; 1.37 M reads/minute). === Summary === Total read pairs processed: 63,320 Read 1 with adapter: 63,314 (100.0%) Read 2 with adapter: 63,320 (100.0%) == Read fate breakdown == Pairs that were too short: 26 (0.0%) Pairs discarded as untrimmed: 6 (0.0%) Pairs written (passing filters): 63,288 (99.9%) Total basepairs processed: 27,416,005 bp Read 1: 15,537,284 bp Read 2: 11,878,721 bp Total written (filtered): 3,995,627 bp (14.6%) Read 1: 2,017,019 bp Read 2: 1,978,608 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 61964 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 989.4 0 2 1 4 3 247.3 1 1 2 6 1 15.5 1 0 0 1 7 2 3.9 2 2 9 3 0.2 2 2 1 10 2 0.1 3 2 15 1 0.0 4 1 16 4 0.0 4 4 17 3 0.0 5 2 1 20 89 0.0 6 86 3 21 37 0.0 6 37 22 38 0.0 6 37 1 23 34 0.0 6 34 24 24 0.0 6 24 25 23 0.0 6 23 26 17 0.0 6 16 1 27 11 0.0 6 9 1 0 0 0 0 1 28 12 0.0 6 12 29 9 0.0 6 9 30 5 0.0 6 5 31 5 0.0 6 5 32 6 0.0 6 6 33 7 0.0 6 6 1 34 3 0.0 6 3 35 6 0.0 6 6 36 3 0.0 6 3 37 6 0.0 6 6 38 3 0.0 6 2 1 39 4 0.0 6 4 40 3 0.0 6 3 41 5 0.0 6 4 1 42 4 0.0 6 3 1 43 4 0.0 6 4 44 7 0.0 6 5 2 45 2 0.0 6 2 46 5 0.0 6 5 47 7 0.0 6 7 48 2 0.0 6 2 49 3 0.0 6 2 0 0 0 1 50 7 0.0 6 7 51 5 0.0 6 5 52 4 0.0 6 3 1 53 9 0.0 6 8 0 1 54 3 0.0 6 3 55 5 0.0 6 5 56 3 0.0 6 3 57 6 0.0 6 6 58 6 0.0 6 6 59 6 0.0 6 6 60 5 0.0 6 4 1 61 4 0.0 6 4 62 9 0.0 6 7 2 63 4 0.0 6 4 64 3 0.0 6 3 65 5 0.0 6 5 66 7 0.0 6 7 67 7 0.0 6 7 68 4 0.0 6 4 69 6 0.0 6 6 70 3 0.0 6 3 71 13 0.0 6 13 72 7 0.0 6 7 73 8 0.0 6 8 74 4 0.0 6 4 75 6 0.0 6 6 76 12 0.0 6 10 2 77 9 0.0 6 9 78 7 0.0 6 7 79 5 0.0 6 5 80 5 0.0 6 4 1 81 8 0.0 6 8 82 8 0.0 6 8 83 10 0.0 6 10 84 11 0.0 6 10 1 85 11 0.0 6 11 86 7 0.0 6 6 0 0 0 0 1 87 8 0.0 6 8 88 10 0.0 6 10 89 10 0.0 6 10 90 9 0.0 6 8 1 91 5 0.0 6 5 92 2 0.0 6 2 93 8 0.0 6 8 94 7 0.0 6 6 1 95 3 0.0 6 3 96 19 0.0 6 17 0 2 97 6 0.0 6 5 1 98 16 0.0 6 16 99 10 0.0 6 10 100 8 0.0 6 7 0 0 0 0 1 101 12 0.0 6 11 1 102 14 0.0 6 14 103 12 0.0 6 11 1 104 13 0.0 6 13 105 18 0.0 6 18 106 10 0.0 6 9 1 107 13 0.0 6 13 108 13 0.0 6 12 0 1 109 19 0.0 6 19 110 18 0.0 6 18 111 24 0.0 6 23 0 0 1 112 17 0.0 6 17 113 22 0.0 6 22 114 19 0.0 6 19 115 15 0.0 6 13 1 0 0 0 0 1 116 18 0.0 6 18 117 18 0.0 6 18 118 24 0.0 6 23 0 0 1 119 20 0.0 6 20 120 18 0.0 6 18 121 23 0.0 6 21 2 122 18 0.0 6 18 123 19 0.0 6 19 124 13 0.0 6 13 125 23 0.0 6 22 1 126 22 0.0 6 21 1 127 21 0.0 6 20 1 128 21 0.0 6 21 129 30 0.0 6 28 2 130 31 0.0 6 30 1 131 35 0.0 6 35 132 36 0.0 6 35 1 133 34 0.0 6 33 1 134 28 0.0 6 28 135 47 0.0 6 46 1 136 44 0.0 6 42 2 137 34 0.0 6 33 1 138 29 0.0 6 28 1 139 43 0.0 6 43 140 39 0.0 6 38 0 1 141 63 0.0 6 62 1 142 130 0.0 6 128 2 143 184 0.0 6 184 144 247 0.0 6 247 145 172 0.0 6 170 0 1 0 1 146 180 0.0 6 178 2 147 245 0.0 6 244 1 148 161 0.0 6 160 1 149 166 0.0 6 163 3 150 177 0.0 6 177 151 148 0.0 6 148 152 163 0.0 6 163 153 160 0.0 6 160 154 128 0.0 6 124 4 155 156 0.0 6 155 0 1 156 182 0.0 6 179 3 157 152 0.0 6 152 158 157 0.0 6 154 2 1 159 175 0.0 6 173 2 160 161 0.0 6 161 161 174 0.0 6 168 6 162 180 0.0 6 180 163 204 0.0 6 202 2 164 190 0.0 6 185 5 165 223 0.0 6 220 3 166 191 0.0 6 188 2 1 167 192 0.0 6 189 3 168 198 0.0 6 196 2 169 211 0.0 6 208 2 0 0 0 1 170 252 0.0 6 249 3 171 224 0.0 6 220 4 172 203 0.0 6 202 1 173 233 0.0 6 232 1 174 258 0.0 6 255 3 175 230 0.0 6 227 3 176 244 0.0 6 239 5 177 290 0.0 6 287 2 0 0 1 178 246 0.0 6 240 6 179 257 0.0 6 254 3 180 538 0.0 6 534 3 0 0 0 0 1 181 316 0.0 6 312 4 182 272 0.0 6 272 183 317 0.0 6 313 4 184 264 0.0 6 262 2 185 298 0.0 6 297 0 0 0 0 0 1 186 329 0.0 6 324 5 187 341 0.0 6 338 3 188 348 0.0 6 346 1 1 189 328 0.0 6 323 5 190 326 0.0 6 323 3 191 368 0.0 6 365 3 192 353 0.0 6 351 1 0 0 1 193 414 0.0 6 412 2 194 489 0.0 6 485 4 195 568 0.0 6 562 6 196 568 0.0 6 563 5 197 680 0.0 6 676 4 198 869 0.0 6 862 7 199 798 0.0 6 791 6 0 0 0 0 1 200 672 0.0 6 669 3 201 633 0.0 6 627 6 202 611 0.0 6 605 6 203 585 0.0 6 576 7 1 0 0 1 204 810 0.0 6 807 3 205 663 0.0 6 661 2 206 676 0.0 6 669 6 1 207 913 0.0 6 909 3 0 1 208 711 0.0 6 710 1 209 790 0.0 6 785 4 0 0 1 210 678 0.0 6 672 5 0 0 0 1 211 704 0.0 6 700 4 212 848 0.0 6 842 5 0 0 0 0 1 213 862 0.0 6 856 6 214 879 0.0 6 873 5 0 0 0 1 215 949 0.0 6 940 8 0 0 0 0 1 216 1128 0.0 6 1124 3 0 0 0 0 1 217 875 0.0 6 868 6 0 0 1 218 836 0.0 6 828 7 0 0 1 219 949 0.0 6 942 6 0 0 1 220 813 0.0 6 807 6 221 694 0.0 6 686 7 1 222 664 0.0 6 658 6 223 706 0.0 6 699 7 224 741 0.0 6 739 1 0 0 0 1 225 732 0.0 6 729 2 0 0 0 0 1 226 941 0.0 6 931 8 2 227 829 0.0 6 819 8 0 0 0 2 228 756 0.0 6 751 5 229 640 0.0 6 634 6 230 645 0.0 6 640 5 231 683 0.0 6 679 4 232 667 0.0 6 658 8 0 0 1 233 667 0.0 6 662 4 0 0 1 234 656 0.0 6 652 2 1 1 235 582 0.0 6 580 2 236 538 0.0 6 535 2 1 237 567 0.0 6 562 5 238 525 0.0 6 520 2 2 0 0 1 239 525 0.0 6 521 3 0 1 240 589 0.0 6 588 1 241 472 0.0 6 466 5 0 0 0 0 1 242 497 0.0 6 495 2 243 497 0.0 6 495 2 244 497 0.0 6 493 3 1 245 448 0.0 6 445 3 246 370 0.0 6 363 6 0 0 0 0 1 247 329 0.0 6 325 3 1 248 310 0.0 6 308 2 249 373 0.0 6 370 3 250 342 0.0 6 341 1 251 316 0.0 6 313 2 1 252 311 0.0 6 306 4 0 0 0 1 253 285 0.0 6 282 3 254 312 0.0 6 307 4 0 1 255 265 0.0 6 259 6 256 296 0.0 6 294 2 257 275 0.0 6 273 2 258 299 0.0 6 296 2 1 259 280 0.0 6 278 2 260 301 0.0 6 300 0 0 0 0 0 1 261 220 0.0 6 217 2 1 262 192 0.0 6 191 1 263 219 0.0 6 219 264 180 0.0 6 179 0 1 265 172 0.0 6 171 1 266 161 0.0 6 160 1 267 148 0.0 6 148 268 157 0.0 6 156 1 269 182 0.0 6 180 2 270 178 0.0 6 178 271 150 0.0 6 148 2 272 136 0.0 6 136 273 119 0.0 6 119 274 165 0.0 6 161 4 275 181 0.0 6 179 2 276 250 0.0 6 247 3 277 299 0.0 6 296 3 278 371 0.0 6 364 6 1 279 1284 0.0 6 1272 11 1 280 2183 0.0 6 2155 27 1 281 19 0.0 6 15 3 1 283 1 0.0 6 1 284 2 0.0 6 1 0 0 1 286 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1350 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 32 0.0 6 30 2 70 1 0.0 6 1 71 1 0.0 6 1 72 6 0.0 6 6 73 7 0.0 6 7 74 2 0.0 6 2 75 5 0.0 6 5 76 5 0.0 6 5 77 4 0.0 6 4 78 6 0.0 6 6 79 6 0.0 6 6 80 2 0.0 6 2 81 3 0.0 6 3 82 6 0.0 6 6 83 1 0.0 6 1 84 2 0.0 6 2 85 5 0.0 6 4 1 86 3 0.0 6 3 87 5 0.0 6 5 88 2 0.0 6 2 89 3 0.0 6 3 90 2 0.0 6 2 91 5 0.0 6 5 92 5 0.0 6 5 93 2 0.0 6 2 94 11 0.0 6 11 95 2 0.0 6 2 96 6 0.0 6 6 97 8 0.0 6 8 99 4 0.0 6 4 100 7 0.0 6 7 101 7 0.0 6 7 102 4 0.0 6 4 103 8 0.0 6 8 104 8 0.0 6 8 105 3 0.0 6 3 106 3 0.0 6 3 107 4 0.0 6 4 108 13 0.0 6 13 109 16 0.0 6 16 110 9 0.0 6 9 111 3 0.0 6 3 112 5 0.0 6 5 113 4 0.0 6 4 114 4 0.0 6 4 115 5 0.0 6 5 116 6 0.0 6 6 117 4 0.0 6 4 118 2 0.0 6 2 119 4 0.0 6 4 120 4 0.0 6 4 121 7 0.0 6 7 122 3 0.0 6 3 123 8 0.0 6 8 124 9 0.0 6 9 125 7 0.0 6 7 126 5 0.0 6 5 127 10 0.0 6 10 128 5 0.0 6 5 129 5 0.0 6 5 130 11 0.0 6 11 131 5 0.0 6 5 132 5 0.0 6 5 133 9 0.0 6 9 134 9 0.0 6 9 135 5 0.0 6 5 136 5 0.0 6 5 137 7 0.0 6 7 138 9 0.0 6 9 139 5 0.0 6 5 140 5 0.0 6 5 141 5 0.0 6 5 142 7 0.0 6 7 143 8 0.0 6 8 144 13 0.0 6 13 145 7 0.0 6 6 1 147 4 0.0 6 4 148 12 0.0 6 12 149 10 0.0 6 10 150 5 0.0 6 5 151 9 0.0 6 9 152 9 0.0 6 9 153 6 0.0 6 6 154 4 0.0 6 4 155 5 0.0 6 5 156 11 0.0 6 11 157 11 0.0 6 11 158 14 0.0 6 14 159 4 0.0 6 4 160 5 0.0 6 5 161 7 0.0 6 7 162 8 0.0 6 8 163 9 0.0 6 9 164 6 0.0 6 6 165 11 0.0 6 11 166 9 0.0 6 9 167 14 0.0 6 13 0 0 0 0 0 1 168 14 0.0 6 14 169 16 0.0 6 16 170 11 0.0 6 11 171 7 0.0 6 7 172 11 0.0 6 11 173 9 0.0 6 9 174 10 0.0 6 10 175 11 0.0 6 11 176 11 0.0 6 11 177 15 0.0 6 15 178 11 0.0 6 11 179 13 0.0 6 13 180 9 0.0 6 9 181 8 0.0 6 8 182 8 0.0 6 8 183 10 0.0 6 10 184 18 0.0 6 18 185 6 0.0 6 6 186 12 0.0 6 12 187 15 0.0 6 15 188 9 0.0 6 9 189 8 0.0 6 8 190 9 0.0 6 9 191 10 0.0 6 10 192 2 0.0 6 2 193 8 0.0 6 8 194 7 0.0 6 7 195 5 0.0 6 5 196 11 0.0 6 11 197 5 0.0 6 5 198 6 0.0 6 6 199 2 0.0 6 2 200 15 0.0 6 15 201 14 0.0 6 14 202 13 0.0 6 13 203 7 0.0 6 7 204 15 0.0 6 15 205 4 0.0 6 4 206 7 0.0 6 7 207 7 0.0 6 7 208 8 0.0 6 8 209 8 0.0 6 8 210 6 0.0 6 6 211 9 0.0 6 9 212 6 0.0 6 6 213 7 0.0 6 7 214 8 0.0 6 8 215 5 0.0 6 5 216 5 0.0 6 5 217 5 0.0 6 5 218 13 0.0 6 13 219 10 0.0 6 10 220 11 0.0 6 11 221 7 0.0 6 7 222 5 0.0 6 5 223 5 0.0 6 5 224 7 0.0 6 7 225 11 0.0 6 10 1 226 5 0.0 6 5 227 7 0.0 6 7 228 3 0.0 6 3 229 10 0.0 6 10 230 10 0.0 6 10 231 6 0.0 6 6 232 7 0.0 6 6 0 0 0 0 0 1 233 6 0.0 6 6 234 5 0.0 6 5 235 6 0.0 6 6 236 9 0.0 6 9 237 5 0.0 6 5 238 2 0.0 6 2 239 9 0.0 6 9 240 6 0.0 6 6 241 5 0.0 6 5 242 4 0.0 6 4 243 3 0.0 6 3 244 3 0.0 6 3 245 5 0.0 6 5 246 4 0.0 6 4 247 3 0.0 6 3 248 5 0.0 6 5 249 7 0.0 6 7 250 3 0.0 6 3 251 2 0.0 6 2 252 2 0.0 6 2 253 3 0.0 6 3 254 1 0.0 6 1 255 3 0.0 6 3 256 3 0.0 6 3 257 3 0.0 6 3 258 2 0.0 6 2 259 1 0.0 6 1 260 1 0.0 6 1 261 2 0.0 6 2 262 4 0.0 6 4 263 3 0.0 6 3 265 3 0.0 6 3 266 4 0.0 6 4 269 1 0.0 6 1 270 5 0.0 6 5 271 1 0.0 6 1 272 3 0.0 6 3 273 1 0.0 6 1 274 1 0.0 6 1 275 2 0.0 6 2 276 1 0.0 6 1 280 1 0.0 6 1 285 1 0.0 6 1 286 2 0.0 6 2 287 2 0.0 6 2 289 1 0.0 6 1 291 1 0.0 6 1 294 1 0.0 6 1 295 1 0.0 6 1 296 1 0.0 6 1 300 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 62305 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 989.4 0 1 5 1 61.8 1 1 7 1 3.9 2 1 9 1 0.2 2 1 10 4 0.1 3 4 11 2 0.0 3 2 12 11 0.0 3 10 1 13 4 0.0 3 4 14 22 0.0 4 19 1 0 2 15 6 0.0 4 4 1 1 16 8 0.0 4 7 1 17 46 0.0 5 43 2 1 18 30 0.0 5 27 3 19 24 0.0 5 23 1 20 84 0.0 6 82 2 21 34 0.0 6 30 4 22 22 0.0 6 22 23 23 0.0 6 23 24 14 0.0 6 13 1 25 21 0.0 6 21 26 18 0.0 6 18 27 12 0.0 6 12 28 26 0.0 6 26 29 13 0.0 6 10 1 1 0 1 30 15 0.0 6 13 1 0 0 0 0 1 31 24 0.0 6 21 2 1 32 16 0.0 6 12 3 0 0 0 1 33 10 0.0 6 9 1 34 23 0.0 6 21 2 35 14 0.0 6 14 36 16 0.0 6 16 37 14 0.0 6 14 38 19 0.0 6 17 2 39 15 0.0 6 13 1 0 0 1 40 12 0.0 6 11 1 41 22 0.0 6 18 4 42 11 0.0 6 10 1 43 15 0.0 6 14 1 44 18 0.0 6 17 1 45 17 0.0 6 13 4 46 22 0.0 6 19 2 1 47 14 0.0 6 14 48 21 0.0 6 20 1 49 25 0.0 6 23 2 50 17 0.0 6 17 51 20 0.0 6 18 2 52 19 0.0 6 19 53 28 0.0 6 24 3 1 54 19 0.0 6 18 1 55 25 0.0 6 24 1 56 21 0.0 6 20 1 57 30 0.0 6 26 2 2 58 20 0.0 6 19 0 1 59 31 0.0 6 28 2 1 60 22 0.0 6 20 2 61 25 0.0 6 25 62 30 0.0 6 29 1 63 25 0.0 6 25 64 34 0.0 6 32 2 65 37 0.0 6 35 2 66 37 0.0 6 35 2 67 53 0.0 6 45 5 2 1 68 146 0.0 6 137 8 1 69 56 0.0 6 53 2 1 70 77 0.0 6 71 6 71 51 0.0 6 47 4 72 43 0.0 6 41 0 1 0 1 73 39 0.0 6 37 2 74 37 0.0 6 36 1 75 49 0.0 6 48 1 76 31 0.0 6 29 1 0 0 0 0 1 77 58 0.0 6 55 2 0 0 0 1 78 41 0.0 6 38 3 79 55 0.0 6 51 4 80 46 0.0 6 44 2 81 52 0.0 6 50 1 1 82 80 0.0 6 73 4 2 1 83 65 0.0 6 62 2 1 84 75 0.0 6 72 3 85 59 0.0 6 55 3 1 86 63 0.0 6 60 3 87 36 0.0 6 31 5 88 54 0.0 6 48 5 0 0 0 0 1 89 59 0.0 6 56 2 0 0 0 0 1 90 50 0.0 6 47 2 0 0 1 91 66 0.0 6 61 5 92 72 0.0 6 68 4 93 81 0.0 6 73 8 94 90 0.0 6 85 5 95 80 0.0 6 76 3 0 0 1 96 90 0.0 6 85 4 0 0 1 97 96 0.0 6 91 3 1 0 0 0 1 98 100 0.0 6 96 4 99 86 0.0 6 84 1 0 0 0 0 1 100 91 0.0 6 88 2 0 0 1 101 84 0.0 6 79 4 0 1 102 93 0.0 6 91 2 103 119 0.0 6 116 3 104 121 0.0 6 117 2 0 1 0 1 105 177 0.0 6 171 3 0 2 0 0 1 106 121 0.0 6 116 5 107 126 0.0 6 122 4 108 111 0.0 6 105 6 109 126 0.0 6 122 3 0 0 0 0 1 110 120 0.0 6 114 5 0 0 1 111 133 0.0 6 129 3 0 0 0 1 112 173 0.0 6 172 1 113 170 0.0 6 159 10 0 1 114 141 0.0 6 133 5 0 1 0 2 115 165 0.0 6 157 7 0 1 116 204 0.0 6 195 8 0 0 0 1 117 167 0.0 6 161 6 118 198 0.0 6 194 2 0 0 1 0 1 119 210 0.0 6 203 6 1 120 192 0.0 6 189 3 121 181 0.0 6 175 6 122 191 0.0 6 184 5 0 1 0 0 1 123 201 0.0 6 195 6 124 217 0.0 6 209 7 0 0 0 1 125 260 0.0 6 256 4 126 267 0.0 6 255 11 0 0 1 127 260 0.0 6 256 3 0 0 1 128 317 0.0 6 309 5 2 1 129 326 0.0 6 319 7 130 377 0.0 6 361 15 1 131 361 0.0 6 353 6 0 2 132 399 0.0 6 390 8 0 1 133 444 0.0 6 424 18 0 1 0 0 1 134 462 0.0 6 449 12 1 135 453 0.0 6 443 9 1 136 464 0.0 6 444 17 0 0 2 1 137 455 0.0 6 443 10 2 138 466 0.0 6 456 10 139 477 0.0 6 466 9 1 1 140 661 0.0 6 648 11 0 0 0 1 1 141 662 0.0 6 653 8 0 0 1 142 709 0.0 6 696 10 0 0 0 1 2 143 732 0.0 6 722 8 1 0 0 0 1 144 719 0.0 6 709 8 0 2 145 773 0.0 6 763 7 0 1 0 0 2 146 818 0.0 6 797 18 2 0 1 147 844 0.0 6 819 19 2 2 2 148 877 0.0 6 862 12 0 2 0 0 1 149 981 0.0 6 951 25 1 2 1 1 150 1140 0.0 6 1123 12 0 1 3 0 1 151 1392 0.0 6 1369 22 0 0 0 0 1 152 1071 0.0 6 1050 19 2 153 1235 0.0 6 1198 30 0 1 0 3 3 154 1289 0.0 6 1264 20 0 0 1 1 3 155 990 0.0 6 969 20 1 156 1101 0.0 6 1085 12 1 0 2 0 1 157 1011 0.0 6 991 13 1 1 2 2 1 158 1098 0.0 6 1078 13 2 1 1 3 159 1056 0.0 6 1041 8 1 0 1 2 3 160 962 0.0 6 945 15 0 1 0 1 161 977 0.0 6 961 14 0 1 0 0 1 162 870 0.0 6 846 20 1 0 0 1 2 163 783 0.0 6 770 9 1 1 1 0 1 164 846 0.0 6 833 8 1 0 2 1 1 165 826 0.0 6 810 15 1 166 762 0.0 6 750 11 0 0 0 1 167 931 0.0 6 923 6 1 0 0 1 168 825 0.0 6 810 12 0 1 0 2 169 898 0.0 6 888 7 0 0 1 0 2 170 800 0.0 6 791 7 1 1 171 784 0.0 6 772 8 1 1 0 2 172 895 0.0 6 877 14 1 0 1 2 173 845 0.0 6 831 9 1 1 0 2 1 174 907 0.0 6 894 11 1 0 0 0 1 175 841 0.0 6 832 9 176 791 0.0 6 785 5 0 0 0 0 1 177 902 0.0 6 896 6 178 702 0.0 6 691 11 179 647 0.0 6 637 10 180 535 0.0 6 526 7 1 1 181 547 0.0 6 544 3 182 495 0.0 6 487 4 0 0 1 2 1 183 487 0.0 6 480 4 1 0 0 1 1 184 743 0.0 6 735 7 0 0 1 185 919 0.0 6 915 4 186 742 0.0 6 738 4 187 566 0.0 6 559 5 1 1 188 566 0.0 6 558 6 1 0 0 1 189 505 0.0 6 499 5 0 0 1 190 516 0.0 6 510 5 0 0 1 191 451 0.0 6 445 5 0 0 1 192 472 0.0 6 465 7 193 336 0.0 6 332 3 1 194 319 0.0 6 313 5 0 1 195 331 0.0 6 324 7 196 361 0.0 6 354 5 0 0 0 0 2 197 403 0.0 6 396 6 0 0 0 1 198 369 0.0 6 367 1 0 0 0 1 199 355 0.0 6 344 11 200 257 0.0 6 253 4 201 235 0.0 6 230 5 202 196 0.0 6 194 1 0 1 203 210 0.0 6 205 5 204 170 0.0 6 170 205 159 0.0 6 159 206 138 0.0 6 137 1 207 136 0.0 6 136 208 136 0.0 6 135 1 209 136 0.0 6 135 1 210 86 0.0 6 85 1 211 107 0.0 6 105 2 212 85 0.0 6 83 2 213 90 0.0 6 89 1 214 99 0.0 6 96 3 215 106 0.0 6 104 2 216 79 0.0 6 78 1 217 75 0.0 6 72 3 218 57 0.0 6 57 219 58 0.0 6 54 2 0 1 0 1 220 40 0.0 6 38 2 221 35 0.0 6 34 1 222 70 0.0 6 69 1 223 55 0.0 6 51 3 0 0 0 0 1 224 46 0.0 6 46 225 52 0.0 6 50 2 226 25 0.0 6 25 227 37 0.0 6 37 228 41 0.0 6 41 229 34 0.0 6 34 230 13 0.0 6 13 231 21 0.0 6 20 1 232 20 0.0 6 20 233 23 0.0 6 22 1 234 18 0.0 6 18 235 9 0.0 6 9 236 8 0.0 6 6 0 1 1 237 7 0.0 6 6 1 238 7 0.0 6 7 239 5 0.0 6 5 240 1 0.0 6 0 1 241 8 0.0 6 8 242 12 0.0 6 12 243 6 0.0 6 6 244 7 0.0 6 7 245 4 0.0 6 4 246 4 0.0 6 4 248 4 0.0 6 4 249 2 0.0 6 2 250 3 0.0 6 3 252 5 0.0 6 4 1 253 1 0.0 6 1 279 19 0.0 6 18 1 280 108 0.0 6 107 0 1 281 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1015 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 3 99 989.4 0 99 55 2 0.0 6 2 58 1 0.0 6 1 61 1 0.0 6 1 66 1 0.0 6 1 70 2 0.0 6 2 71 1 0.0 6 1 72 6 0.0 6 6 73 6 0.0 6 6 74 5 0.0 6 4 0 0 1 75 5 0.0 6 5 76 12 0.0 6 12 77 3 0.0 6 3 78 5 0.0 6 5 79 7 0.0 6 7 80 4 0.0 6 4 81 3 0.0 6 3 82 4 0.0 6 4 83 1 0.0 6 1 84 3 0.0 6 3 85 4 0.0 6 4 86 5 0.0 6 5 87 4 0.0 6 4 88 3 0.0 6 3 89 3 0.0 6 3 90 4 0.0 6 4 91 7 0.0 6 7 92 4 0.0 6 4 93 3 0.0 6 3 94 5 0.0 6 5 95 5 0.0 6 5 96 4 0.0 6 4 97 3 0.0 6 3 98 3 0.0 6 3 99 2 0.0 6 1 0 1 100 4 0.0 6 3 1 101 3 0.0 6 3 102 4 0.0 6 4 103 4 0.0 6 4 104 3 0.0 6 3 105 2 0.0 6 2 106 3 0.0 6 3 107 2 0.0 6 2 108 6 0.0 6 6 109 16 0.0 6 16 110 5 0.0 6 5 111 4 0.0 6 4 112 4 0.0 6 4 113 7 0.0 6 7 114 3 0.0 6 3 115 7 0.0 6 7 116 7 0.0 6 7 117 2 0.0 6 2 118 5 0.0 6 5 119 4 0.0 6 3 0 0 0 1 120 2 0.0 6 2 121 3 0.0 6 3 122 6 0.0 6 6 123 3 0.0 6 3 124 8 0.0 6 7 0 0 0 1 125 3 0.0 6 3 126 7 0.0 6 7 127 10 0.0 6 10 128 5 0.0 6 4 1 129 7 0.0 6 7 130 4 0.0 6 4 131 8 0.0 6 8 132 7 0.0 6 7 133 8 0.0 6 8 134 7 0.0 6 7 135 11 0.0 6 11 136 7 0.0 6 7 137 12 0.0 6 12 138 10 0.0 6 10 139 7 0.0 6 7 140 7 0.0 6 7 141 6 0.0 6 6 142 8 0.0 6 8 143 8 0.0 6 8 144 6 0.0 6 6 145 10 0.0 6 10 146 9 0.0 6 9 147 7 0.0 6 7 148 6 0.0 6 6 149 7 0.0 6 7 150 5 0.0 6 5 151 2 0.0 6 2 152 11 0.0 6 11 153 5 0.0 6 5 154 9 0.0 6 8 0 0 0 1 155 8 0.0 6 8 156 9 0.0 6 9 157 10 0.0 6 10 158 12 0.0 6 12 159 11 0.0 6 11 160 5 0.0 6 5 161 11 0.0 6 11 162 17 0.0 6 17 163 13 0.0 6 13 164 14 0.0 6 14 165 9 0.0 6 9 166 9 0.0 6 9 167 10 0.0 6 10 168 6 0.0 6 6 169 13 0.0 6 13 170 8 0.0 6 8 171 10 0.0 6 9 0 0 1 172 14 0.0 6 14 173 11 0.0 6 11 174 12 0.0 6 12 175 10 0.0 6 10 176 8 0.0 6 8 177 13 0.0 6 13 178 12 0.0 6 12 179 8 0.0 6 8 180 5 0.0 6 5 181 5 0.0 6 5 182 4 0.0 6 4 183 7 0.0 6 7 184 9 0.0 6 8 1 185 4 0.0 6 4 186 10 0.0 6 10 187 6 0.0 6 5 0 0 0 1 188 7 0.0 6 7 189 4 0.0 6 4 190 7 0.0 6 7 191 7 0.0 6 7 192 4 0.0 6 4 193 5 0.0 6 5 194 7 0.0 6 6 0 0 0 1 195 10 0.0 6 10 196 7 0.0 6 7 197 5 0.0 6 5 198 3 0.0 6 3 199 6 0.0 6 6 200 9 0.0 6 9 201 4 0.0 6 4 202 4 0.0 6 4 203 2 0.0 6 2 204 2 0.0 6 2 205 4 0.0 6 4 206 2 0.0 6 2 207 6 0.0 6 6 208 3 0.0 6 3 209 1 0.0 6 1 211 3 0.0 6 3 212 4 0.0 6 4 213 2 0.0 6 2 214 3 0.0 6 3 215 1 0.0 6 1 217 1 0.0 6 1 218 1 0.0 6 1 219 2 0.0 6 2 220 2 0.0 6 2 221 1 0.0 6 1 223 1 0.0 6 1 224 1 0.0 6 1 227 2 0.0 6 2 234 1 0.0 6 1 236 1 0.0 6 1 238 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_03_98_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_03_325_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_03_98_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_03_325_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 8.57 s (78 µs/read; 0.77 M reads/minute). === Summary === Total read pairs processed: 109,592 Read 1 with adapter: 109,583 (100.0%) Read 2 with adapter: 109,519 (99.9%) == Read fate breakdown == Pairs that were too short: 1 (0.0%) Pairs discarded as untrimmed: 81 (0.1%) Pairs written (passing filters): 109,510 (99.9%) Total basepairs processed: 56,276,740 bp Read 1: 31,762,534 bp Read 2: 24,514,206 bp Total written (filtered): 6,903,756 bp (12.3%) Read 1: 3,398,796 bp Read 2: 3,504,960 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 109498 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1712.4 0 0 2 4 4 428.1 1 1 3 5 2 107.0 1 2 6 2 26.8 1 0 0 2 7 3 6.7 2 2 0 1 8 2 1.7 2 2 9 5 0.4 2 4 1 10 2 0.1 3 2 14 2 0.0 4 2 15 1 0.0 4 1 16 1 0.0 4 1 20 24 0.0 6 23 1 21 19 0.0 6 19 22 7 0.0 6 6 1 23 2 0.0 6 2 24 9 0.0 6 8 1 25 13 0.0 6 13 26 8 0.0 6 8 27 7 0.0 6 6 1 28 10 0.0 6 10 29 2 0.0 6 2 30 3 0.0 6 3 31 4 0.0 6 4 33 2 0.0 6 2 34 4 0.0 6 4 35 3 0.0 6 3 36 4 0.0 6 3 1 37 3 0.0 6 2 1 38 4 0.0 6 4 39 3 0.0 6 3 40 5 0.0 6 5 41 6 0.0 6 5 1 42 2 0.0 6 2 44 5 0.0 6 5 45 7 0.0 6 7 47 1 0.0 6 1 48 3 0.0 6 2 0 1 49 2 0.0 6 2 50 4 0.0 6 3 1 52 1 0.0 6 1 53 2 0.0 6 2 54 6 0.0 6 5 1 55 1 0.0 6 1 56 8 0.0 6 7 1 57 5 0.0 6 5 58 3 0.0 6 2 1 59 1 0.0 6 1 60 1 0.0 6 1 61 1 0.0 6 1 62 5 0.0 6 4 1 63 3 0.0 6 2 1 64 1 0.0 6 1 65 2 0.0 6 1 1 66 2 0.0 6 1 1 67 3 0.0 6 3 68 6 0.0 6 4 1 1 69 2 0.0 6 2 71 1 0.0 6 1 72 2 0.0 6 1 1 73 1 0.0 6 1 74 1 0.0 6 1 75 1 0.0 6 1 76 1 0.0 6 1 77 7 0.0 6 7 78 8 0.0 6 8 79 3 0.0 6 3 80 1 0.0 6 1 81 1 0.0 6 1 82 3 0.0 6 2 0 1 83 1 0.0 6 1 84 1 0.0 6 1 85 1 0.0 6 1 86 17 0.0 6 17 87 6 0.0 6 6 88 1 0.0 6 1 89 11 0.0 6 11 90 7 0.0 6 7 91 5 0.0 6 5 92 5 0.0 6 4 1 93 6 0.0 6 6 94 3 0.0 6 3 95 4 0.0 6 4 96 1 0.0 6 1 97 8 0.0 6 7 1 98 9 0.0 6 9 99 4 0.0 6 4 100 8 0.0 6 8 101 13 0.0 6 12 1 102 7 0.0 6 6 1 103 1 0.0 6 1 104 6 0.0 6 6 105 3 0.0 6 3 106 7 0.0 6 5 2 107 14 0.0 6 10 3 0 0 1 108 14 0.0 6 14 109 5 0.0 6 5 110 3 0.0 6 3 111 7 0.0 6 6 0 1 112 9 0.0 6 8 1 113 19 0.0 6 19 114 10 0.0 6 10 115 9 0.0 6 9 116 8 0.0 6 7 0 0 1 117 10 0.0 6 10 118 9 0.0 6 8 0 1 119 13 0.0 6 13 120 8 0.0 6 6 2 121 10 0.0 6 9 1 122 32 0.0 6 28 4 123 15 0.0 6 14 1 124 16 0.0 6 15 0 1 125 26 0.0 6 25 1 126 28 0.0 6 26 2 127 10 0.0 6 10 128 30 0.0 6 30 129 19 0.0 6 19 130 13 0.0 6 12 1 131 9 0.0 6 9 132 57 0.0 6 57 133 19 0.0 6 19 134 62 0.0 6 59 3 135 40 0.0 6 39 1 136 17 0.0 6 17 137 47 0.0 6 45 2 138 24 0.0 6 23 1 139 12 0.0 6 12 140 15 0.0 6 13 2 141 31 0.0 6 28 3 142 18 0.0 6 17 1 143 83 0.0 6 82 1 144 86 0.0 6 82 3 1 145 76 0.0 6 74 2 146 53 0.0 6 51 2 147 43 0.0 6 43 148 81 0.0 6 80 0 1 149 76 0.0 6 74 2 150 61 0.0 6 59 2 151 55 0.0 6 55 152 53 0.0 6 50 3 153 52 0.0 6 52 154 41 0.0 6 41 155 29 0.0 6 29 156 51 0.0 6 50 1 157 33 0.0 6 33 158 30 0.0 6 30 159 38 0.0 6 37 0 0 1 160 49 0.0 6 47 1 1 161 46 0.0 6 45 1 162 39 0.0 6 39 163 51 0.0 6 50 1 164 61 0.0 6 58 3 165 48 0.0 6 46 2 166 63 0.0 6 60 1 2 167 63 0.0 6 61 2 168 63 0.0 6 63 169 59 0.0 6 57 1 1 170 84 0.0 6 83 1 171 67 0.0 6 67 172 46 0.0 6 45 1 173 57 0.0 6 55 2 174 70 0.0 6 68 2 175 72 0.0 6 71 1 176 56 0.0 6 56 177 80 0.0 6 77 3 178 54 0.0 6 54 179 49 0.0 6 46 2 1 180 87 0.0 6 86 1 181 113 0.0 6 112 1 182 82 0.0 6 81 1 183 105 0.0 6 100 5 184 77 0.0 6 75 2 185 66 0.0 6 65 1 186 72 0.0 6 70 2 187 97 0.0 6 97 188 99 0.0 6 94 5 189 111 0.0 6 108 3 190 89 0.0 6 84 5 191 130 0.0 6 126 4 192 153 0.0 6 147 6 193 143 0.0 6 137 6 194 144 0.0 6 142 2 195 160 0.0 6 154 5 1 196 166 0.0 6 164 2 197 171 0.0 6 165 6 198 171 0.0 6 168 3 199 166 0.0 6 160 5 1 200 160 0.0 6 154 6 201 161 0.0 6 155 6 202 177 0.0 6 173 4 203 203 0.0 6 198 4 0 1 204 201 0.0 6 195 6 205 222 0.0 6 214 8 206 237 0.0 6 231 6 207 290 0.0 6 280 9 1 208 297 0.0 6 289 8 209 359 0.0 6 350 9 210 366 0.0 6 350 16 211 585 0.0 6 570 15 212 309 0.0 6 302 6 1 213 424 0.0 6 416 7 0 0 0 1 214 607 0.0 6 589 17 0 1 215 235 0.0 6 224 10 0 1 216 572 0.0 6 560 12 217 314 0.0 6 306 7 1 218 245 0.0 6 240 5 219 273 0.0 6 266 7 220 259 0.0 6 256 3 221 240 0.0 6 235 5 222 346 0.0 6 337 9 223 353 0.0 6 340 12 1 224 455 0.0 6 443 10 1 1 225 299 0.0 6 289 9 1 226 1060 0.0 6 1034 23 1 1 1 227 592 0.0 6 579 12 1 228 506 0.0 6 487 18 0 0 0 0 1 229 523 0.0 6 505 18 230 382 0.0 6 368 14 231 367 0.0 6 355 11 1 232 308 0.0 6 301 4 1 1 1 233 330 0.0 6 323 7 234 361 0.0 6 353 8 235 237 0.0 6 226 9 2 236 258 0.0 6 249 9 237 508 0.0 6 497 11 238 219 0.0 6 208 10 1 239 410 0.0 6 385 22 2 1 240 978 0.0 6 953 25 241 421 0.0 6 410 11 242 410 0.0 6 402 8 243 432 0.0 6 420 12 244 407 0.0 6 393 11 3 245 507 0.0 6 490 15 2 246 360 0.0 6 351 9 247 771 0.0 6 751 20 248 397 0.0 6 384 11 1 1 249 736 0.0 6 718 18 250 830 0.0 6 808 21 1 251 660 0.0 6 635 23 1 1 252 1915 0.0 6 1868 47 253 799 0.0 6 772 26 0 1 254 844 0.0 6 829 14 1 255 686 0.0 6 670 16 256 1410 0.0 6 1371 37 2 257 1411 0.0 6 1369 37 5 258 1621 0.0 6 1565 53 2 1 259 2346 0.0 6 2298 47 1 260 1564 0.0 6 1517 46 1 261 2203 0.0 6 2155 44 4 262 1004 0.0 6 966 35 2 1 263 2221 0.0 6 2177 40 3 1 264 984 0.0 6 966 15 2 1 265 660 0.0 6 634 26 266 1290 0.0 6 1262 28 267 1366 0.0 6 1347 19 268 770 0.0 6 741 28 1 269 1375 0.0 6 1339 35 1 270 1083 0.0 6 1055 27 1 271 883 0.0 6 867 16 272 769 0.0 6 746 21 2 273 825 0.0 6 807 17 1 274 951 0.0 6 926 24 1 275 1305 0.0 6 1260 43 2 276 3344 0.0 6 3277 64 3 277 3132 0.0 6 3041 90 1 278 3203 0.0 6 3105 94 4 279 19939 0.0 6 19517 400 18 3 1 280 22571 0.0 6 21933 631 7 281 222 0.0 6 196 20 5 1 282 7 0.0 6 4 0 2 0 1 283 8 0.0 6 4 1 0 1 2 284 4 0.0 6 3 1 285 2 0.0 6 2 286 1 0.0 6 1 288 3 0.0 6 3 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 85 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 3 0.0 6 3 71 1 0.0 6 1 72 4 0.0 6 4 73 2 0.0 6 2 74 2 0.0 6 2 76 4 0.0 6 4 77 1 0.0 6 1 78 1 0.0 6 1 81 2 0.0 6 2 82 1 0.0 6 1 83 1 0.0 6 1 84 1 0.0 6 1 85 1 0.0 6 1 86 1 0.0 6 1 88 1 0.0 6 1 89 1 0.0 6 1 91 1 0.0 6 1 92 1 0.0 6 1 94 1 0.0 6 1 102 1 0.0 6 1 103 1 0.0 6 1 104 1 0.0 6 1 108 1 0.0 6 1 109 3 0.0 6 3 110 1 0.0 6 1 111 1 0.0 6 1 117 1 0.0 6 1 120 1 0.0 6 1 122 1 0.0 6 1 125 1 0.0 6 1 126 1 0.0 6 1 127 1 0.0 6 1 128 1 0.0 6 1 130 1 0.0 6 1 133 1 0.0 6 1 135 2 0.0 6 2 136 1 0.0 6 1 138 1 0.0 6 1 139 1 0.0 6 1 141 3 0.0 6 3 144 2 0.0 6 2 148 1 0.0 6 1 156 2 0.0 6 2 157 2 0.0 6 2 162 1 0.0 6 1 165 1 0.0 6 1 168 2 0.0 6 2 169 1 0.0 6 1 170 1 0.0 6 1 174 1 0.0 6 1 175 1 0.0 6 1 176 1 0.0 6 1 181 1 0.0 6 1 189 1 0.0 6 1 194 1 0.0 6 1 196 1 0.0 6 1 202 2 0.0 6 2 214 1 0.0 6 1 217 1 0.0 6 1 229 1 0.0 6 1 232 1 0.0 6 1 235 1 0.0 6 1 236 1 0.0 6 1 250 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 109461 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1712.4 0 0 2 4 2 428.1 1 1 1 8 1 1.7 2 0 1 10 4 0.1 3 4 11 8 0.0 3 5 2 0 0 1 12 2 0.0 3 0 2 13 7 0.0 3 6 0 0 1 14 10 0.0 4 10 15 1 0.0 4 1 16 13 0.0 4 13 17 14 0.0 5 9 5 18 24 0.0 5 20 4 19 13 0.0 5 10 3 20 74 0.0 6 68 5 1 21 61 0.0 6 59 2 22 33 0.0 6 31 2 23 34 0.0 6 32 2 24 31 0.0 6 30 1 25 32 0.0 6 31 1 26 26 0.0 6 25 1 27 20 0.0 6 19 1 28 18 0.0 6 18 29 19 0.0 6 17 2 30 18 0.0 6 16 1 0 0 1 31 15 0.0 6 15 32 14 0.0 6 12 1 1 33 10 0.0 6 10 34 14 0.0 6 12 2 35 8 0.0 6 8 36 8 0.0 6 7 1 37 24 0.0 6 24 38 10 0.0 6 10 39 8 0.0 6 8 40 10 0.0 6 9 1 41 10 0.0 6 8 2 42 9 0.0 6 7 2 43 17 0.0 6 12 4 0 0 1 44 22 0.0 6 21 1 45 9 0.0 6 9 46 21 0.0 6 17 2 2 47 30 0.0 6 28 2 48 11 0.0 6 10 1 49 18 0.0 6 15 2 1 50 20 0.0 6 16 2 2 51 12 0.0 6 10 2 52 42 0.0 6 39 2 0 1 53 28 0.0 6 25 2 0 0 1 54 33 0.0 6 28 5 55 25 0.0 6 22 3 56 15 0.0 6 14 1 57 28 0.0 6 27 1 58 26 0.0 6 24 2 59 6 0.0 6 4 1 1 60 21 0.0 6 19 1 1 61 10 0.0 6 8 2 62 12 0.0 6 11 1 63 20 0.0 6 19 1 64 23 0.0 6 19 4 65 8 0.0 6 7 1 66 27 0.0 6 23 3 1 67 217 0.0 6 208 7 1 0 1 68 53 0.0 6 46 6 0 1 69 95 0.0 6 93 2 70 14 0.0 6 14 71 33 0.0 6 31 2 72 33 0.0 6 31 1 1 73 15 0.0 6 14 1 74 18 0.0 6 17 1 75 14 0.0 6 13 1 76 29 0.0 6 27 1 0 1 77 26 0.0 6 24 2 78 27 0.0 6 22 5 79 17 0.0 6 16 1 80 22 0.0 6 20 1 0 0 0 1 81 19 0.0 6 18 1 82 45 0.0 6 40 4 1 83 94 0.0 6 86 5 2 0 0 1 84 56 0.0 6 48 6 1 0 1 85 26 0.0 6 23 3 86 25 0.0 6 22 2 1 87 54 0.0 6 47 7 88 30 0.0 6 27 3 89 33 0.0 6 31 2 90 57 0.0 6 48 8 0 0 1 91 40 0.0 6 39 0 1 92 27 0.0 6 26 1 93 69 0.0 6 66 3 94 73 0.0 6 67 5 0 1 95 45 0.0 6 41 4 96 56 0.0 6 50 5 0 0 1 97 44 0.0 6 42 2 98 43 0.0 6 40 3 99 69 0.0 6 65 2 2 100 67 0.0 6 62 5 101 51 0.0 6 48 2 1 102 84 0.0 6 77 6 1 103 101 0.0 6 94 6 1 104 93 0.0 6 90 3 105 75 0.0 6 65 7 2 0 1 106 48 0.0 6 42 5 1 107 68 0.0 6 63 5 108 51 0.0 6 47 4 109 64 0.0 6 61 3 110 62 0.0 6 54 7 1 111 73 0.0 6 67 6 112 55 0.0 6 52 3 113 55 0.0 6 47 8 114 95 0.0 6 87 7 1 115 78 0.0 6 76 1 0 1 116 73 0.0 6 68 4 0 0 1 117 53 0.0 6 48 5 118 72 0.0 6 66 6 119 77 0.0 6 72 3 1 0 1 120 97 0.0 6 92 4 1 121 70 0.0 6 66 4 122 87 0.0 6 86 1 123 104 0.0 6 97 4 3 124 115 0.0 6 110 4 0 0 1 125 120 0.0 6 111 7 2 126 97 0.0 6 88 8 0 0 1 127 142 0.0 6 137 3 2 128 97 0.0 6 92 4 0 0 0 1 129 69 0.0 6 66 2 1 130 72 0.0 6 69 3 131 147 0.0 6 141 5 0 0 1 132 201 0.0 6 199 2 133 142 0.0 6 136 6 134 267 0.0 6 258 8 0 0 1 135 362 0.0 6 344 16 1 1 136 176 0.0 6 170 6 137 216 0.0 6 209 7 138 99 0.0 6 98 1 139 220 0.0 6 215 5 140 363 0.0 6 350 12 1 141 301 0.0 6 284 15 1 0 1 142 405 0.0 6 393 11 0 0 0 0 1 143 348 0.0 6 337 10 1 144 542 0.0 6 526 15 0 1 145 326 0.0 6 314 10 1 0 1 146 313 0.0 6 301 12 147 224 0.0 6 219 5 148 328 0.0 6 319 9 149 510 0.0 6 491 18 0 0 1 150 917 0.0 6 878 38 1 151 375 0.0 6 365 7 2 0 1 152 727 0.0 6 695 29 3 153 489 0.0 6 464 22 0 0 2 1 154 438 0.0 6 419 19 155 349 0.0 6 330 16 0 1 1 0 1 156 370 0.0 6 346 20 1 1 1 0 1 157 551 0.0 6 529 21 1 158 600 0.0 6 579 19 1 0 1 159 273 0.0 6 262 10 0 0 1 160 522 0.0 6 508 14 161 299 0.0 6 282 12 2 0 3 162 283 0.0 6 272 9 0 1 1 163 526 0.0 6 513 11 1 0 1 164 573 0.0 6 556 14 1 2 165 644 0.0 6 616 24 2 0 2 166 779 0.0 6 749 28 1 0 1 167 559 0.0 6 541 17 1 168 975 0.0 6 946 27 2 169 459 0.0 6 440 15 2 0 2 170 557 0.0 6 540 16 1 171 970 0.0 6 944 23 2 0 1 172 454 0.0 6 434 18 1 0 1 173 614 0.0 6 595 18 0 1 174 943 0.0 6 920 22 0 1 175 1213 0.0 6 1177 34 1 1 176 1025 0.0 6 987 32 3 3 177 1024 0.0 6 993 31 178 746 0.0 6 722 22 1 0 1 179 888 0.0 6 866 19 1 1 0 0 1 180 873 0.0 6 846 26 1 181 968 0.0 6 942 22 2 1 1 182 306 0.0 6 293 13 183 962 0.0 6 935 27 184 1000 0.0 6 967 29 2 1 1 185 1116 0.0 6 1074 40 2 186 1951 0.0 6 1894 51 3 0 3 187 1704 0.0 6 1653 48 2 0 1 188 1820 0.0 6 1764 49 4 1 2 189 1741 0.0 6 1680 57 2 0 1 0 1 190 2188 0.0 6 2103 79 4 1 0 1 191 2544 0.0 6 2474 65 3 1 1 192 1292 0.0 6 1252 38 1 0 1 193 1581 0.0 6 1540 37 2 0 1 0 1 194 1579 0.0 6 1528 47 2 1 1 195 1790 0.0 6 1735 54 0 0 1 196 2870 0.0 6 2783 79 4 1 2 1 197 1113 0.0 6 1054 54 0 1 3 0 1 198 2446 0.0 6 2375 67 1 0 3 199 2335 0.0 6 2262 66 4 1 2 200 1824 0.0 6 1780 40 3 0 1 201 2355 0.0 6 2285 65 3 0 2 202 2040 0.0 6 1991 41 6 0 1 1 203 1312 0.0 6 1276 34 1 0 1 204 1636 0.0 6 1586 46 4 205 1408 0.0 6 1368 36 2 1 1 206 1022 0.0 6 987 35 207 1240 0.0 6 1199 40 1 208 1775 0.0 6 1715 58 0 0 2 209 493 0.0 6 480 12 1 210 610 0.0 6 581 24 3 2 211 1273 0.0 6 1241 30 1 0 1 212 1057 0.0 6 1022 31 1 0 3 213 650 0.0 6 625 23 1 0 1 214 1336 0.0 6 1293 39 1 0 2 0 1 215 1309 0.0 6 1278 27 3 1 216 1594 0.0 6 1556 37 0 0 1 217 964 0.0 6 941 22 0 0 1 218 658 0.0 6 640 18 219 650 0.0 6 628 22 220 327 0.0 6 308 18 1 221 692 0.0 6 667 23 1 1 222 944 0.0 6 917 25 1 0 1 223 955 0.0 6 931 21 2 1 224 683 0.0 6 664 17 0 2 225 695 0.0 6 675 19 1 226 911 0.0 6 871 39 1 227 1548 0.0 6 1495 53 228 1235 0.0 6 1194 35 3 2 1 229 1760 0.0 6 1704 53 0 1 2 230 3008 0.0 6 2919 86 3 231 996 0.0 6 966 29 1 232 1800 0.0 6 1753 45 1 0 1 233 410 0.0 6 393 17 234 575 0.0 6 557 16 2 235 339 0.0 6 329 10 236 226 0.0 6 219 7 237 245 0.0 6 241 3 0 1 238 1006 0.0 6 986 20 239 142 0.0 6 138 4 240 204 0.0 6 195 7 1 1 241 441 0.0 6 431 9 0 0 1 242 403 0.0 6 388 15 243 211 0.0 6 206 5 244 196 0.0 6 193 3 245 434 0.0 6 422 12 246 325 0.0 6 318 7 247 277 0.0 6 272 5 248 329 0.0 6 314 15 249 149 0.0 6 146 3 250 82 0.0 6 79 3 251 413 0.0 6 408 4 1 252 46 0.0 6 46 253 40 0.0 6 38 2 254 33 0.0 6 32 0 0 1 255 10 0.0 6 9 1 256 7 0.0 6 7 257 3 0.0 6 3 258 1 0.0 6 1 259 2 0.0 6 2 260 2 0.0 6 2 263 2 0.0 6 2 264 1 0.0 6 1 265 3 0.0 6 3 274 1 0.0 6 1 277 6 0.0 6 5 0 1 278 64 0.0 6 63 1 279 188 0.0 6 180 8 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 58 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 2 0.0 6 2 73 2 0.0 6 2 74 1 0.0 6 1 75 2 0.0 6 2 77 4 0.0 6 4 79 1 0.0 6 1 87 1 0.0 6 1 88 1 0.0 6 1 92 2 0.0 6 2 94 2 0.0 6 2 95 1 0.0 6 1 96 1 0.0 6 1 97 1 0.0 6 1 103 1 0.0 6 1 108 1 0.0 6 1 109 3 0.0 6 3 110 6 0.0 6 6 120 1 0.0 6 1 122 1 0.0 6 1 126 1 0.0 6 1 128 1 0.0 6 1 135 1 0.0 6 1 137 2 0.0 6 2 150 1 0.0 6 1 157 1 0.0 6 1 158 1 0.0 6 1 163 1 0.0 6 1 169 1 0.0 6 1 170 1 0.0 6 1 173 1 0.0 6 1 174 2 0.0 6 2 177 1 0.0 6 1 178 1 0.0 6 1 182 1 0.0 6 1 185 1 0.0 6 1 191 2 0.0 6 2 195 2 0.0 6 2 196 1 0.0 6 1 201 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_04_99_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_04_326_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_04_99_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_04_326_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.81 s (37 µs/read; 1.62 M reads/minute). === Summary === Total read pairs processed: 76,130 Read 1 with adapter: 76,122 (100.0%) Read 2 with adapter: 76,083 (99.9%) == Read fate breakdown == Pairs that were too short: 45 (0.1%) Pairs discarded as untrimmed: 55 (0.1%) Pairs written (passing filters): 76,030 (99.9%) Total basepairs processed: 32,545,303 bp Read 1: 18,353,210 bp Read 2: 14,192,093 bp Total written (filtered): 4,686,785 bp (14.4%) Read 1: 2,438,097 bp Read 2: 2,248,688 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 74226 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 1189.5 0 2 1 4 1 297.4 1 0 1 7 2 4.6 2 2 9 2 0.3 2 1 1 10 1 0.1 3 1 11 1 0.0 3 1 13 1 0.0 3 1 14 1 0.0 4 1 16 4 0.0 4 3 1 17 7 0.0 5 7 19 2 0.0 5 2 20 114 0.0 6 108 6 21 46 0.0 6 44 2 22 67 0.0 6 66 1 23 44 0.0 6 42 2 24 22 0.0 6 20 2 25 16 0.0 6 16 26 18 0.0 6 18 27 15 0.0 6 14 1 28 15 0.0 6 14 1 29 10 0.0 6 9 1 30 8 0.0 6 8 31 7 0.0 6 7 32 3 0.0 6 2 0 0 1 33 8 0.0 6 8 34 5 0.0 6 5 35 7 0.0 6 7 36 4 0.0 6 4 37 6 0.0 6 6 38 3 0.0 6 3 39 9 0.0 6 9 40 9 0.0 6 7 2 41 9 0.0 6 9 42 9 0.0 6 8 1 43 9 0.0 6 9 44 8 0.0 6 7 1 45 7 0.0 6 6 0 1 46 6 0.0 6 5 1 47 4 0.0 6 4 48 7 0.0 6 7 49 4 0.0 6 4 50 7 0.0 6 7 51 5 0.0 6 5 52 7 0.0 6 5 2 53 4 0.0 6 4 54 12 0.0 6 12 55 7 0.0 6 7 56 5 0.0 6 5 57 2 0.0 6 2 58 4 0.0 6 3 1 59 7 0.0 6 7 60 3 0.0 6 3 61 4 0.0 6 4 62 8 0.0 6 7 1 63 6 0.0 6 6 64 8 0.0 6 8 65 9 0.0 6 9 66 8 0.0 6 7 1 67 12 0.0 6 12 68 4 0.0 6 4 69 8 0.0 6 8 70 5 0.0 6 5 71 14 0.0 6 14 72 9 0.0 6 9 73 11 0.0 6 11 74 7 0.0 6 6 1 75 12 0.0 6 12 76 19 0.0 6 19 77 8 0.0 6 7 1 78 6 0.0 6 6 79 8 0.0 6 8 80 5 0.0 6 4 1 81 10 0.0 6 10 82 13 0.0 6 12 0 0 0 0 1 83 16 0.0 6 16 84 18 0.0 6 18 85 13 0.0 6 13 86 12 0.0 6 11 1 87 8 0.0 6 8 88 9 0.0 6 8 1 89 13 0.0 6 10 3 90 15 0.0 6 13 2 91 6 0.0 6 6 92 8 0.0 6 7 1 93 10 0.0 6 9 1 94 5 0.0 6 5 95 7 0.0 6 7 96 12 0.0 6 11 0 0 0 0 1 97 10 0.0 6 10 98 16 0.0 6 16 99 18 0.0 6 18 100 11 0.0 6 11 101 24 0.0 6 24 102 21 0.0 6 21 103 11 0.0 6 11 104 16 0.0 6 16 105 18 0.0 6 18 106 25 0.0 6 23 2 107 21 0.0 6 20 1 108 19 0.0 6 17 2 109 20 0.0 6 19 1 110 15 0.0 6 15 111 16 0.0 6 15 1 112 18 0.0 6 18 113 21 0.0 6 21 114 26 0.0 6 25 1 115 18 0.0 6 16 2 116 34 0.0 6 34 117 30 0.0 6 30 118 31 0.0 6 30 1 119 20 0.0 6 19 1 120 23 0.0 6 22 1 121 27 0.0 6 25 2 122 18 0.0 6 16 1 0 0 0 1 123 33 0.0 6 32 1 124 34 0.0 6 33 0 1 125 27 0.0 6 25 2 126 35 0.0 6 34 0 1 127 28 0.0 6 28 128 28 0.0 6 28 129 27 0.0 6 25 2 130 30 0.0 6 29 1 131 36 0.0 6 35 1 132 37 0.0 6 36 1 133 31 0.0 6 30 1 134 37 0.0 6 37 135 43 0.0 6 43 136 45 0.0 6 43 2 137 42 0.0 6 42 138 55 0.0 6 55 139 77 0.0 6 75 2 140 183 0.0 6 183 141 376 0.0 6 373 2 0 1 142 437 0.0 6 436 1 143 342 0.0 6 339 2 0 0 0 0 1 144 281 0.0 6 280 1 145 371 0.0 6 371 146 358 0.0 6 356 2 147 272 0.0 6 270 2 148 332 0.0 6 331 1 149 376 0.0 6 373 3 150 418 0.0 6 417 1 151 327 0.0 6 325 1 0 0 0 0 1 152 286 0.0 6 285 1 153 272 0.0 6 269 3 154 341 0.0 6 339 2 155 336 0.0 6 336 156 281 0.0 6 281 157 297 0.0 6 296 1 158 290 0.0 6 289 0 1 159 354 0.0 6 351 2 1 160 316 0.0 6 316 161 272 0.0 6 269 3 162 227 0.0 6 225 2 163 323 0.0 6 319 4 164 290 0.0 6 289 1 165 187 0.0 6 186 1 166 377 0.0 6 377 167 271 0.0 6 269 2 168 355 0.0 6 351 3 1 169 288 0.0 6 288 170 436 0.0 6 436 171 347 0.0 6 343 4 172 356 0.0 6 354 1 0 0 1 173 431 0.0 6 429 1 1 174 259 0.0 6 258 0 0 0 0 0 1 175 340 0.0 6 337 3 176 375 0.0 6 373 1 0 0 1 177 332 0.0 6 331 0 0 0 0 0 1 178 661 0.0 6 656 5 179 398 0.0 6 395 2 0 1 180 354 0.0 6 348 5 0 0 1 181 371 0.0 6 366 4 0 0 0 0 1 182 339 0.0 6 338 0 0 1 183 357 0.0 6 356 0 0 0 0 1 184 506 0.0 6 505 0 0 1 185 371 0.0 6 368 3 186 364 0.0 6 362 2 187 374 0.0 6 369 3 2 188 462 0.0 6 459 3 189 346 0.0 6 342 4 190 414 0.0 6 409 5 191 401 0.0 6 398 2 1 192 528 0.0 6 515 12 0 0 0 0 1 193 657 0.0 6 656 1 194 477 0.0 6 473 3 0 0 0 1 195 538 0.0 6 535 3 196 719 0.0 6 713 6 197 691 0.0 6 680 10 1 198 860 0.0 6 852 7 0 0 0 0 1 199 935 0.0 6 924 10 1 200 812 0.0 6 803 9 201 587 0.0 6 580 5 1 0 0 1 202 825 0.0 6 817 6 1 1 203 631 0.0 6 621 9 1 204 657 0.0 6 649 6 1 0 1 205 778 0.0 6 773 4 1 206 803 0.0 6 797 6 207 946 0.0 6 938 7 1 208 814 0.0 6 808 4 1 0 0 0 1 209 855 0.0 6 853 2 210 726 0.0 6 722 3 1 211 670 0.0 6 659 9 1 0 0 1 212 914 0.0 6 899 15 213 902 0.0 6 896 4 0 0 1 1 214 1034 0.0 6 1024 8 0 0 0 0 2 215 1054 0.0 6 1049 4 0 0 0 0 1 216 1192 0.0 6 1181 8 1 2 217 984 0.0 6 976 6 1 0 1 218 951 0.0 6 942 8 0 0 0 0 1 219 993 0.0 6 979 11 0 0 0 2 1 220 988 0.0 6 974 10 1 1 1 1 221 754 0.0 6 746 6 1 0 0 1 222 729 0.0 6 719 8 0 0 1 1 223 787 0.0 6 781 5 0 1 224 901 0.0 6 893 6 0 0 0 1 1 225 816 0.0 6 808 7 0 0 1 226 971 0.0 6 960 9 0 1 0 0 1 227 938 0.0 6 931 5 0 0 1 1 228 731 0.0 6 722 8 1 229 670 0.0 6 667 2 1 230 686 0.0 6 680 5 1 231 790 0.0 6 784 5 1 232 737 0.0 6 733 4 233 809 0.0 6 804 5 234 716 0.0 6 711 3 0 0 0 2 235 655 0.0 6 647 6 0 0 1 1 236 593 0.0 6 588 5 237 657 0.0 6 653 4 238 615 0.0 6 608 6 0 1 239 629 0.0 6 624 2 0 0 0 1 2 240 657 0.0 6 654 3 241 559 0.0 6 555 3 1 242 540 0.0 6 533 5 0 2 243 532 0.0 6 528 2 1 1 244 552 0.0 6 549 3 245 462 0.0 6 460 2 246 448 0.0 6 443 5 247 425 0.0 6 422 3 248 375 0.0 6 371 3 0 0 1 249 365 0.0 6 363 1 0 0 0 1 250 379 0.0 6 375 4 251 390 0.0 6 385 4 0 0 0 0 1 252 368 0.0 6 365 3 253 307 0.0 6 305 2 254 323 0.0 6 320 3 255 325 0.0 6 323 1 1 256 315 0.0 6 314 0 1 257 300 0.0 6 299 1 258 315 0.0 6 311 2 1 0 0 1 259 286 0.0 6 284 2 260 361 0.0 6 358 3 261 351 0.0 6 346 5 262 233 0.0 6 233 263 252 0.0 6 251 1 264 238 0.0 6 237 1 265 194 0.0 6 193 1 266 205 0.0 6 203 2 267 224 0.0 6 221 3 268 187 0.0 6 187 269 214 0.0 6 212 2 270 257 0.0 6 256 1 271 179 0.0 6 177 1 0 0 0 0 1 272 161 0.0 6 159 2 273 158 0.0 6 155 3 274 187 0.0 6 185 2 275 237 0.0 6 234 2 1 276 290 0.0 6 289 1 277 318 0.0 6 316 2 278 422 0.0 6 417 5 279 1549 0.0 6 1529 19 0 0 0 1 280 1929 0.0 6 1910 18 1 281 13 0.0 6 12 1 282 3 0.0 6 3 288 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1896 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 47 0.0 6 46 1 70 2 0.0 6 2 71 7 0.0 6 7 72 15 0.0 6 15 73 17 0.0 6 16 1 74 6 0.0 6 6 75 6 0.0 6 6 76 19 0.0 6 19 77 11 0.0 6 11 78 9 0.0 6 9 79 18 0.0 6 18 80 11 0.0 6 11 81 16 0.0 6 16 82 9 0.0 6 9 83 5 0.0 6 5 84 9 0.0 6 9 85 7 0.0 6 7 86 8 0.0 6 8 87 7 0.0 6 7 88 2 0.0 6 2 89 9 0.0 6 9 90 7 0.0 6 7 91 9 0.0 6 9 92 5 0.0 6 5 93 7 0.0 6 7 94 9 0.0 6 9 95 3 0.0 6 3 96 8 0.0 6 8 97 16 0.0 6 16 98 6 0.0 6 6 99 5 0.0 6 5 100 9 0.0 6 9 101 13 0.0 6 13 102 8 0.0 6 8 103 15 0.0 6 15 104 10 0.0 6 10 105 8 0.0 6 7 0 0 0 0 0 1 106 6 0.0 6 6 107 12 0.0 6 12 108 11 0.0 6 11 109 12 0.0 6 12 110 8 0.0 6 8 111 5 0.0 6 5 112 5 0.0 6 5 113 13 0.0 6 13 114 3 0.0 6 3 115 13 0.0 6 13 116 5 0.0 6 5 117 2 0.0 6 2 118 10 0.0 6 10 119 10 0.0 6 10 120 6 0.0 6 6 121 7 0.0 6 7 122 8 0.0 6 8 123 14 0.0 6 13 0 0 0 0 0 1 124 9 0.0 6 9 125 6 0.0 6 6 126 8 0.0 6 8 127 9 0.0 6 9 128 8 0.0 6 8 129 12 0.0 6 12 130 8 0.0 6 8 131 8 0.0 6 8 132 6 0.0 6 6 133 16 0.0 6 16 134 5 0.0 6 5 135 15 0.0 6 15 136 14 0.0 6 14 137 6 0.0 6 6 138 7 0.0 6 7 139 4 0.0 6 4 140 13 0.0 6 13 141 13 0.0 6 13 142 7 0.0 6 7 143 6 0.0 6 6 144 10 0.0 6 10 145 7 0.0 6 7 146 10 0.0 6 10 147 6 0.0 6 6 148 10 0.0 6 10 149 10 0.0 6 10 150 5 0.0 6 5 151 15 0.0 6 15 152 11 0.0 6 11 153 11 0.0 6 11 154 16 0.0 6 16 155 24 0.0 6 24 156 10 0.0 6 10 157 11 0.0 6 11 158 10 0.0 6 10 159 9 0.0 6 9 160 8 0.0 6 8 161 25 0.0 6 25 162 21 0.0 6 21 163 11 0.0 6 11 164 10 0.0 6 10 165 5 0.0 6 5 166 4 0.0 6 4 167 12 0.0 6 12 168 10 0.0 6 10 169 14 0.0 6 14 170 11 0.0 6 11 171 16 0.0 6 16 172 15 0.0 6 15 173 9 0.0 6 9 174 13 0.0 6 13 175 5 0.0 6 5 176 10 0.0 6 10 177 9 0.0 6 9 178 13 0.0 6 13 179 17 0.0 6 17 180 9 0.0 6 9 181 14 0.0 6 14 182 4 0.0 6 4 183 8 0.0 6 8 184 16 0.0 6 16 185 2 0.0 6 2 186 8 0.0 6 8 187 7 0.0 6 7 188 12 0.0 6 12 189 11 0.0 6 11 190 7 0.0 6 7 191 14 0.0 6 14 192 12 0.0 6 12 193 5 0.0 6 5 194 10 0.0 6 10 195 12 0.0 6 11 1 196 9 0.0 6 9 197 9 0.0 6 9 198 10 0.0 6 10 199 9 0.0 6 9 200 14 0.0 6 14 201 14 0.0 6 14 202 13 0.0 6 13 203 12 0.0 6 12 204 15 0.0 6 15 205 12 0.0 6 12 206 5 0.0 6 5 207 10 0.0 6 10 208 23 0.0 6 23 209 10 0.0 6 10 210 11 0.0 6 11 211 11 0.0 6 11 212 16 0.0 6 16 213 17 0.0 6 17 214 8 0.0 6 8 215 5 0.0 6 5 216 8 0.0 6 8 217 10 0.0 6 9 1 218 4 0.0 6 4 219 3 0.0 6 3 220 7 0.0 6 7 221 6 0.0 6 6 222 7 0.0 6 7 223 10 0.0 6 10 224 7 0.0 6 7 225 9 0.0 6 9 226 12 0.0 6 12 227 17 0.0 6 17 228 5 0.0 6 5 229 10 0.0 6 10 230 10 0.0 6 10 231 6 0.0 6 5 1 232 8 0.0 6 8 233 5 0.0 6 5 234 12 0.0 6 12 235 9 0.0 6 9 236 8 0.0 6 8 237 2 0.0 6 2 239 4 0.0 6 4 240 5 0.0 6 5 241 7 0.0 6 7 242 5 0.0 6 5 243 6 0.0 6 6 244 5 0.0 6 5 245 8 0.0 6 8 246 9 0.0 6 9 247 14 0.0 6 14 248 10 0.0 6 10 249 6 0.0 6 6 250 4 0.0 6 4 251 6 0.0 6 6 252 4 0.0 6 4 253 5 0.0 6 5 254 5 0.0 6 5 255 6 0.0 6 6 256 5 0.0 6 5 257 2 0.0 6 2 258 6 0.0 6 6 259 9 0.0 6 9 260 8 0.0 6 8 261 6 0.0 6 6 262 3 0.0 6 3 263 3 0.0 6 3 264 1 0.0 6 1 265 2 0.0 6 2 267 4 0.0 6 4 268 4 0.0 6 4 269 4 0.0 6 4 270 3 0.0 6 3 271 3 0.0 6 3 272 2 0.0 6 2 273 1 0.0 6 1 274 4 0.0 6 4 275 7 0.0 6 7 276 2 0.0 6 2 278 2 0.0 6 2 280 1 0.0 6 1 281 2 0.0 6 2 282 2 0.0 6 2 283 1 0.0 6 1 284 1 0.0 6 1 286 2 0.0 6 2 288 3 0.0 6 3 289 3 0.0 6 3 290 1 0.0 6 1 291 1 0.0 6 1 292 1 0.0 6 1 293 2 0.0 6 2 295 2 0.0 6 2 297 1 0.0 6 1 303 1 0.0 6 1 310 1 0.0 6 1 311 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 74597 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1189.5 0 1 5 1 74.3 1 1 6 1 18.6 1 1 9 1 0.3 2 1 11 3 0.0 3 3 12 7 0.0 3 7 13 7 0.0 3 6 1 14 9 0.0 4 9 15 2 0.0 4 1 1 16 8 0.0 4 7 1 17 28 0.0 5 26 2 18 6 0.0 5 3 3 19 83 0.0 5 78 4 1 20 117 0.0 6 112 5 21 60 0.0 6 59 1 22 41 0.0 6 40 1 23 46 0.0 6 41 4 1 24 30 0.0 6 28 2 25 30 0.0 6 28 2 26 27 0.0 6 25 2 27 18 0.0 6 15 1 1 1 28 26 0.0 6 23 3 29 13 0.0 6 12 1 30 16 0.0 6 15 1 31 24 0.0 6 22 2 32 18 0.0 6 16 2 33 24 0.0 6 21 3 34 13 0.0 6 10 2 1 35 17 0.0 6 12 4 0 0 1 36 14 0.0 6 12 1 1 37 15 0.0 6 15 38 21 0.0 6 20 1 39 19 0.0 6 18 1 40 17 0.0 6 16 1 41 11 0.0 6 11 42 20 0.0 6 18 2 43 21 0.0 6 20 0 0 1 44 14 0.0 6 13 1 45 22 0.0 6 21 1 46 21 0.0 6 19 1 0 0 0 1 47 22 0.0 6 22 48 22 0.0 6 21 1 49 14 0.0 6 12 2 50 29 0.0 6 28 1 51 23 0.0 6 23 52 17 0.0 6 17 53 19 0.0 6 16 3 54 22 0.0 6 21 0 0 0 1 55 24 0.0 6 22 2 56 30 0.0 6 25 4 1 57 28 0.0 6 26 1 1 58 28 0.0 6 27 1 59 32 0.0 6 27 4 0 1 60 27 0.0 6 26 1 61 33 0.0 6 31 2 62 38 0.0 6 36 1 0 1 63 36 0.0 6 34 2 64 33 0.0 6 32 1 65 45 0.0 6 34 8 1 1 1 66 43 0.0 6 38 3 2 67 43 0.0 6 40 3 68 42 0.0 6 41 1 69 59 0.0 6 55 2 1 0 1 70 163 0.0 6 153 7 2 0 1 71 79 0.0 6 75 4 72 85 0.0 6 83 0 0 1 0 1 73 44 0.0 6 41 3 74 65 0.0 6 63 1 0 0 0 1 75 50 0.0 6 48 2 76 47 0.0 6 43 4 77 46 0.0 6 44 2 78 46 0.0 6 43 1 0 0 0 1 1 79 70 0.0 6 64 4 0 1 1 80 69 0.0 6 65 3 1 81 59 0.0 6 53 5 1 82 81 0.0 6 78 2 1 83 80 0.0 6 79 1 84 73 0.0 6 71 1 0 0 0 1 85 77 0.0 6 73 1 3 86 90 0.0 6 82 8 87 50 0.0 6 47 3 88 53 0.0 6 50 3 89 61 0.0 6 55 6 90 73 0.0 6 65 6 0 0 1 1 91 65 0.0 6 64 1 92 67 0.0 6 64 3 93 95 0.0 6 90 4 1 94 81 0.0 6 79 0 1 0 0 0 1 95 106 0.0 6 102 2 1 1 96 103 0.0 6 94 7 2 97 71 0.0 6 66 3 1 1 98 100 0.0 6 97 3 99 103 0.0 6 95 7 1 100 105 0.0 6 102 1 0 1 0 0 1 101 98 0.0 6 96 2 102 89 0.0 6 88 1 103 94 0.0 6 90 3 1 104 105 0.0 6 96 7 1 0 0 0 1 105 131 0.0 6 128 2 0 0 0 1 106 130 0.0 6 125 5 107 233 0.0 6 219 11 1 1 0 1 108 161 0.0 6 154 6 1 109 146 0.0 6 144 2 110 139 0.0 6 136 2 0 0 1 111 134 0.0 6 128 6 112 157 0.0 6 152 4 0 0 0 1 113 160 0.0 6 159 1 114 147 0.0 6 144 2 0 0 1 115 198 0.0 6 191 6 0 0 1 116 190 0.0 6 185 5 117 159 0.0 6 155 3 0 0 0 1 118 217 0.0 6 212 5 119 206 0.0 6 200 5 0 0 0 1 120 224 0.0 6 219 3 0 0 1 0 1 121 223 0.0 6 212 9 0 0 1 0 1 122 209 0.0 6 206 2 1 123 201 0.0 6 194 6 0 0 0 1 124 203 0.0 6 198 3 1 0 0 1 125 247 0.0 6 244 2 1 126 274 0.0 6 264 7 2 0 0 1 127 271 0.0 6 262 6 0 1 1 0 1 128 327 0.0 6 310 13 3 1 129 312 0.0 6 298 12 0 0 2 130 420 0.0 6 408 11 0 1 131 405 0.0 6 397 5 0 1 0 2 132 375 0.0 6 366 8 0 0 0 1 133 448 0.0 6 432 16 134 473 0.0 6 457 13 0 2 0 0 1 135 500 0.0 6 480 18 1 1 136 546 0.0 6 525 20 0 0 0 1 137 608 0.0 6 587 17 1 0 1 2 138 613 0.0 6 601 11 0 0 0 0 1 139 654 0.0 6 638 14 0 0 1 0 1 140 811 0.0 6 799 12 141 804 0.0 6 790 12 0 0 2 142 866 0.0 6 847 15 2 0 1 1 143 812 0.0 6 799 10 1 1 0 1 144 904 0.0 6 889 13 1 0 0 0 1 145 1010 0.0 6 993 15 0 1 1 146 925 0.0 6 900 21 0 0 0 2 2 147 999 0.0 6 978 17 1 1 1 1 148 1125 0.0 6 1103 19 1 1 0 1 149 1167 0.0 6 1137 24 1 0 2 1 2 150 1242 0.0 6 1216 20 2 0 2 0 2 151 1260 0.0 6 1228 26 2 0 1 1 2 152 1339 0.0 6 1311 22 3 0 1 1 1 153 1711 0.0 6 1681 23 2 3 0 1 1 154 1399 0.0 6 1381 14 2 0 1 0 1 155 1559 0.0 6 1516 33 4 0 2 2 2 156 1551 0.0 6 1516 28 0 1 1 4 1 157 1346 0.0 6 1325 20 0 0 0 0 1 158 1428 0.0 6 1403 21 2 1 1 159 1257 0.0 6 1233 20 2 0 1 0 1 160 1305 0.0 6 1274 23 3 1 3 0 1 161 1175 0.0 6 1156 15 0 1 2 0 1 162 1177 0.0 6 1157 18 0 1 1 163 1147 0.0 6 1128 14 2 0 1 1 1 164 1066 0.0 6 1048 15 0 0 1 1 1 165 988 0.0 6 978 6 1 2 0 1 166 1080 0.0 6 1053 23 1 1 2 167 974 0.0 6 956 15 0 1 0 1 1 168 977 0.0 6 956 16 1 0 1 2 1 169 1178 0.0 6 1162 14 0 0 1 0 1 170 901 0.0 6 882 16 1 1 0 1 171 1183 0.0 6 1166 13 2 1 1 172 964 0.0 6 949 14 0 1 173 919 0.0 6 910 7 0 1 0 0 1 174 1087 0.0 6 1073 11 1 1 1 175 1125 0.0 6 1107 15 0 1 2 176 979 0.0 6 962 14 0 0 1 1 1 177 941 0.0 6 931 8 2 178 906 0.0 6 893 11 1 0 0 0 1 179 900 0.0 6 885 14 0 0 0 1 180 740 0.0 6 730 7 1 0 0 1 1 181 750 0.0 6 741 6 1 0 1 1 182 635 0.0 6 633 1 0 0 1 183 572 0.0 6 566 3 0 1 1 1 184 581 0.0 6 574 6 0 0 1 185 557 0.0 6 549 6 0 0 1 1 186 780 0.0 6 772 7 1 187 1052 0.0 6 1040 11 0 0 0 0 1 188 871 0.0 6 859 10 2 189 625 0.0 6 623 2 190 543 0.0 6 537 6 191 582 0.0 6 576 6 192 504 0.0 6 496 8 193 438 0.0 6 432 5 0 0 0 1 194 515 0.0 6 508 6 1 195 382 0.0 6 377 5 196 369 0.0 6 365 4 197 311 0.0 6 308 2 0 0 1 198 257 0.0 6 254 2 0 1 199 363 0.0 6 357 5 0 0 0 0 1 200 335 0.0 6 331 4 201 305 0.0 6 300 5 202 257 0.0 6 253 4 203 240 0.0 6 238 2 204 217 0.0 6 217 205 209 0.0 6 206 3 206 198 0.0 6 195 3 207 171 0.0 6 170 1 208 176 0.0 6 175 1 209 138 0.0 6 138 210 145 0.0 6 142 2 0 0 0 1 211 153 0.0 6 151 1 0 0 0 0 1 212 121 0.0 6 120 1 213 98 0.0 6 97 1 214 109 0.0 6 107 2 215 119 0.0 6 116 2 0 1 216 120 0.0 6 118 2 217 126 0.0 6 122 4 218 89 0.0 6 87 2 219 87 0.0 6 85 2 220 65 0.0 6 65 221 62 0.0 6 61 1 222 53 0.0 6 53 223 43 0.0 6 43 224 50 0.0 6 49 1 225 37 0.0 6 36 1 226 46 0.0 6 44 2 227 48 0.0 6 47 1 228 38 0.0 6 38 229 29 0.0 6 27 2 230 49 0.0 6 48 0 0 0 1 231 36 0.0 6 34 2 232 37 0.0 6 36 1 233 19 0.0 6 19 234 19 0.0 6 19 235 26 0.0 6 25 1 236 9 0.0 6 9 237 11 0.0 6 11 238 8 0.0 6 8 239 3 0.0 6 3 240 9 0.0 6 8 1 241 8 0.0 6 7 1 242 4 0.0 6 3 1 243 4 0.0 6 4 244 7 0.0 6 6 1 245 12 0.0 6 12 246 8 0.0 6 7 0 0 1 247 5 0.0 6 5 248 4 0.0 6 3 1 249 5 0.0 6 5 250 4 0.0 6 4 251 8 0.0 6 8 252 4 0.0 6 4 253 2 0.0 6 2 254 12 0.0 6 11 1 255 3 0.0 6 3 260 1 0.0 6 1 279 2 0.0 6 2 280 3 0.0 6 3 281 11 0.0 6 11 282 101 0.0 6 99 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1486 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 14 1 0.0 4 0 0 0 0 1 67 2 0.0 6 2 68 8 0.0 6 7 1 69 8 0.0 6 8 70 15 0.0 6 15 71 18 0.0 6 18 72 11 0.0 6 11 73 9 0.0 6 9 74 15 0.0 6 15 75 13 0.0 6 12 0 0 1 76 11 0.0 6 11 77 15 0.0 6 15 78 12 0.0 6 12 79 15 0.0 6 15 80 7 0.0 6 7 81 10 0.0 6 10 82 12 0.0 6 12 83 12 0.0 6 12 84 12 0.0 6 12 85 8 0.0 6 8 86 5 0.0 6 5 87 10 0.0 6 10 88 14 0.0 6 13 1 89 17 0.0 6 17 90 11 0.0 6 11 91 7 0.0 6 7 92 13 0.0 6 13 93 10 0.0 6 10 94 5 0.0 6 5 95 13 0.0 6 13 96 7 0.0 6 7 97 9 0.0 6 9 98 6 0.0 6 6 99 11 0.0 6 11 100 15 0.0 6 15 101 15 0.0 6 15 102 7 0.0 6 7 103 6 0.0 6 6 104 12 0.0 6 12 105 9 0.0 6 9 106 8 0.0 6 8 107 27 0.0 6 27 108 14 0.0 6 13 0 0 0 1 109 12 0.0 6 11 0 1 110 8 0.0 6 8 111 6 0.0 6 6 112 10 0.0 6 10 113 17 0.0 6 17 114 6 0.0 6 6 115 5 0.0 6 5 116 2 0.0 6 2 117 13 0.0 6 13 118 6 0.0 6 6 119 14 0.0 6 14 120 7 0.0 6 7 121 3 0.0 6 3 122 13 0.0 6 13 123 8 0.0 6 8 124 13 0.0 6 13 125 13 0.0 6 12 0 0 0 1 126 7 0.0 6 7 127 8 0.0 6 8 128 12 0.0 6 12 129 22 0.0 6 22 130 9 0.0 6 9 131 42 0.0 6 42 132 7 0.0 6 7 133 4 0.0 6 4 134 20 0.0 6 20 135 12 0.0 6 12 136 12 0.0 6 12 137 13 0.0 6 13 138 10 0.0 6 10 139 13 0.0 6 13 140 10 0.0 6 10 141 10 0.0 6 10 142 6 0.0 6 6 143 5 0.0 6 5 144 13 0.0 6 13 145 15 0.0 6 15 146 15 0.0 6 15 147 2 0.0 6 2 148 9 0.0 6 9 149 10 0.0 6 10 150 8 0.0 6 8 151 5 0.0 6 5 152 12 0.0 6 12 153 21 0.0 6 21 154 5 0.0 6 5 155 12 0.0 6 12 156 10 0.0 6 10 157 9 0.0 6 8 0 0 0 1 158 7 0.0 6 7 159 9 0.0 6 9 160 14 0.0 6 13 0 0 1 161 11 0.0 6 11 162 11 0.0 6 11 163 8 0.0 6 8 164 9 0.0 6 9 165 13 0.0 6 13 166 11 0.0 6 11 167 9 0.0 6 9 168 78 0.0 6 78 169 14 0.0 6 14 170 10 0.0 6 10 171 12 0.0 6 11 0 0 1 172 10 0.0 6 8 0 0 1 1 173 5 0.0 6 5 174 6 0.0 6 5 0 0 0 1 175 12 0.0 6 12 176 13 0.0 6 13 177 10 0.0 6 10 178 4 0.0 6 4 179 8 0.0 6 8 180 6 0.0 6 6 181 5 0.0 6 5 182 12 0.0 6 12 183 10 0.0 6 10 184 10 0.0 6 10 185 9 0.0 6 8 1 186 6 0.0 6 6 187 2 0.0 6 2 188 9 0.0 6 9 189 8 0.0 6 8 190 5 0.0 6 5 191 4 0.0 6 4 192 7 0.0 6 6 0 0 0 1 193 3 0.0 6 3 194 10 0.0 6 10 195 5 0.0 6 5 196 2 0.0 6 2 197 9 0.0 6 9 198 12 0.0 6 12 199 2 0.0 6 2 200 7 0.0 6 7 201 1 0.0 6 1 202 7 0.0 6 7 203 4 0.0 6 4 204 3 0.0 6 3 205 1 0.0 6 1 206 5 0.0 6 5 207 4 0.0 6 4 208 6 0.0 6 6 209 4 0.0 6 4 210 4 0.0 6 4 211 6 0.0 6 6 212 2 0.0 6 2 213 1 0.0 6 1 214 2 0.0 6 2 216 2 0.0 6 2 217 1 0.0 6 1 218 1 0.0 6 1 220 1 0.0 6 1 221 1 0.0 6 1 222 2 0.0 6 2 224 3 0.0 6 3 229 1 0.0 6 1 230 2 0.0 6 2 232 2 0.0 6 2 233 2 0.0 6 2 241 1 0.0 6 1 251 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_05_100_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_05_327_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_05_100_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_05_327_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 6.85 s (90 µs/read; 0.67 M reads/minute). === Summary === Total read pairs processed: 76,461 Read 1 with adapter: 76,456 (100.0%) Read 2 with adapter: 76,423 (100.0%) == Read fate breakdown == Pairs that were too short: 9 (0.0%) Pairs discarded as untrimmed: 41 (0.1%) Pairs written (passing filters): 76,411 (99.9%) Total basepairs processed: 36,875,597 bp Read 1: 20,856,303 bp Read 2: 16,019,294 bp Total written (filtered): 4,698,384 bp (12.7%) Read 1: 2,395,726 bp Read 2: 2,302,658 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 75853 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 6 1194.7 0 0 6 4 2 298.7 1 0 2 6 2 18.7 1 0 0 2 7 4 4.7 2 2 0 2 9 4 0.3 2 2 2 11 1 0.0 3 1 16 3 0.0 4 1 2 17 2 0.0 5 2 20 55 0.0 6 54 1 21 37 0.0 6 36 1 22 24 0.0 6 24 23 13 0.0 6 11 2 24 12 0.0 6 11 1 25 9 0.0 6 8 1 26 9 0.0 6 9 27 5 0.0 6 5 28 11 0.0 6 11 29 3 0.0 6 3 30 3 0.0 6 2 1 31 3 0.0 6 1 2 32 3 0.0 6 3 33 4 0.0 6 4 34 2 0.0 6 2 35 2 0.0 6 2 36 3 0.0 6 2 1 37 1 0.0 6 1 38 2 0.0 6 2 40 4 0.0 6 4 41 1 0.0 6 1 42 5 0.0 6 5 43 3 0.0 6 3 44 3 0.0 6 3 45 3 0.0 6 3 46 1 0.0 6 1 47 2 0.0 6 2 48 3 0.0 6 3 50 6 0.0 6 6 51 4 0.0 6 4 52 3 0.0 6 3 53 5 0.0 6 4 0 0 0 0 1 54 3 0.0 6 2 1 55 1 0.0 6 0 1 56 7 0.0 6 6 1 57 5 0.0 6 4 1 58 5 0.0 6 5 59 4 0.0 6 4 60 3 0.0 6 3 61 2 0.0 6 2 62 7 0.0 6 5 2 63 4 0.0 6 4 64 4 0.0 6 4 65 3 0.0 6 3 66 5 0.0 6 4 1 67 5 0.0 6 5 68 6 0.0 6 6 69 1 0.0 6 1 70 3 0.0 6 3 71 6 0.0 6 6 72 5 0.0 6 5 73 8 0.0 6 8 74 7 0.0 6 7 75 2 0.0 6 2 76 4 0.0 6 4 77 4 0.0 6 4 78 4 0.0 6 4 79 8 0.0 6 8 80 10 0.0 6 10 81 5 0.0 6 5 82 1 0.0 6 1 83 3 0.0 6 3 84 5 0.0 6 4 1 85 4 0.0 6 4 86 6 0.0 6 6 87 7 0.0 6 7 88 9 0.0 6 9 89 10 0.0 6 8 1 0 0 0 0 1 90 4 0.0 6 4 91 4 0.0 6 4 92 5 0.0 6 4 1 93 5 0.0 6 4 1 94 3 0.0 6 2 1 95 2 0.0 6 2 96 5 0.0 6 4 1 97 3 0.0 6 3 98 10 0.0 6 9 1 99 8 0.0 6 8 100 7 0.0 6 7 101 7 0.0 6 6 0 1 102 7 0.0 6 7 103 3 0.0 6 3 104 7 0.0 6 7 105 11 0.0 6 10 1 106 11 0.0 6 11 107 6 0.0 6 4 1 1 108 10 0.0 6 9 1 109 10 0.0 6 10 110 5 0.0 6 4 1 111 12 0.0 6 12 112 13 0.0 6 12 1 113 11 0.0 6 11 114 9 0.0 6 7 2 115 5 0.0 6 5 116 12 0.0 6 10 2 117 9 0.0 6 9 118 10 0.0 6 9 1 119 7 0.0 6 7 120 11 0.0 6 11 121 10 0.0 6 10 122 18 0.0 6 16 1 0 0 0 1 123 19 0.0 6 19 124 15 0.0 6 13 2 125 19 0.0 6 18 1 126 19 0.0 6 17 1 0 0 0 1 127 22 0.0 6 21 0 0 0 1 128 31 0.0 6 28 3 129 26 0.0 6 24 2 130 17 0.0 6 16 1 131 19 0.0 6 19 132 27 0.0 6 27 133 23 0.0 6 23 134 33 0.0 6 33 135 28 0.0 6 28 136 28 0.0 6 28 137 29 0.0 6 29 138 14 0.0 6 13 1 139 25 0.0 6 25 140 34 0.0 6 34 141 83 0.0 6 83 142 129 0.0 6 128 1 143 157 0.0 6 157 144 126 0.0 6 122 3 1 145 105 0.0 6 104 1 146 153 0.0 6 153 147 130 0.0 6 129 1 148 130 0.0 6 129 1 149 133 0.0 6 133 150 164 0.0 6 163 1 151 125 0.0 6 124 1 152 86 0.0 6 86 153 71 0.0 6 71 154 93 0.0 6 93 155 114 0.0 6 112 2 156 76 0.0 6 75 1 157 61 0.0 6 60 1 158 92 0.0 6 92 159 90 0.0 6 88 1 0 1 160 117 0.0 6 117 161 153 0.0 6 152 1 162 145 0.0 6 145 163 95 0.0 6 94 1 164 117 0.0 6 116 1 165 118 0.0 6 118 166 105 0.0 6 105 167 98 0.0 6 97 0 0 1 168 83 0.0 6 83 169 136 0.0 6 136 170 123 0.0 6 121 2 171 127 0.0 6 126 1 172 155 0.0 6 153 2 173 199 0.0 6 192 7 174 109 0.0 6 107 2 175 118 0.0 6 117 0 0 1 176 132 0.0 6 130 2 177 138 0.0 6 136 2 178 148 0.0 6 147 1 179 270 0.0 6 268 2 180 186 0.0 6 185 1 181 139 0.0 6 135 4 182 157 0.0 6 154 3 183 138 0.0 6 136 2 184 162 0.0 6 160 2 185 200 0.0 6 198 2 186 195 0.0 6 195 187 165 0.0 6 164 1 188 156 0.0 6 151 4 0 1 189 160 0.0 6 159 1 190 158 0.0 6 156 2 191 183 0.0 6 180 3 192 181 0.0 6 179 2 193 235 0.0 6 233 2 194 287 0.0 6 279 8 195 323 0.0 6 319 3 0 0 1 196 331 0.0 6 330 1 197 323 0.0 6 315 8 198 447 0.0 6 437 8 2 199 372 0.0 6 368 2 1 1 200 405 0.0 6 398 7 201 322 0.0 6 316 6 202 373 0.0 6 369 3 0 0 0 1 203 361 0.0 6 357 3 0 0 0 1 204 362 0.0 6 357 5 205 355 0.0 6 350 5 206 438 0.0 6 431 6 1 207 495 0.0 6 491 4 208 483 0.0 6 476 7 209 451 0.0 6 446 5 210 380 0.0 6 373 6 0 0 1 211 502 0.0 6 493 9 212 376 0.0 6 367 9 213 615 0.0 6 603 11 0 1 214 477 0.0 6 470 7 215 484 0.0 6 480 4 216 543 0.0 6 540 3 217 476 0.0 6 467 8 0 0 0 0 1 218 448 0.0 6 444 4 219 532 0.0 6 524 8 220 424 0.0 6 417 7 221 497 0.0 6 484 12 1 222 391 0.0 6 384 6 1 223 677 0.0 6 664 13 224 634 0.0 6 626 8 225 565 0.0 6 556 9 226 638 0.0 6 631 7 227 496 0.0 6 488 6 1 0 0 0 1 228 482 0.0 6 475 7 229 423 0.0 6 413 10 230 450 0.0 6 443 6 1 231 501 0.0 6 494 7 232 389 0.0 6 384 5 233 394 0.0 6 388 5 1 234 488 0.0 6 481 7 235 395 0.0 6 391 3 1 236 555 0.0 6 543 12 237 763 0.0 6 751 11 1 238 533 0.0 6 526 6 0 0 0 1 239 584 0.0 6 575 8 1 240 471 0.0 6 461 9 0 0 1 241 546 0.0 6 539 7 242 614 0.0 6 601 12 0 1 243 653 0.0 6 639 13 1 244 627 0.0 6 619 8 245 536 0.0 6 523 13 246 459 0.0 6 448 11 247 600 0.0 6 592 8 248 479 0.0 6 467 12 249 896 0.0 6 875 20 1 250 496 0.0 6 482 14 251 607 0.0 6 593 13 0 1 252 633 0.0 6 624 8 1 253 748 0.0 6 731 16 0 0 0 0 1 254 721 0.0 6 705 14 2 255 575 0.0 6 558 17 256 1245 0.0 6 1215 29 1 257 567 0.0 6 553 14 258 973 0.0 6 946 26 1 259 671 0.0 6 645 26 260 1557 0.0 6 1531 25 1 261 500 0.0 6 485 15 262 418 0.0 6 405 13 263 530 0.0 6 519 11 264 632 0.0 6 616 14 2 265 415 0.0 6 404 11 266 801 0.0 6 784 16 1 267 344 0.0 6 332 12 268 527 0.0 6 517 9 0 0 1 269 664 0.0 6 646 17 1 270 538 0.0 6 530 7 1 271 394 0.0 6 385 9 272 466 0.0 6 453 13 273 889 0.0 6 871 18 274 1161 0.0 6 1138 20 2 1 275 1183 0.0 6 1155 28 276 1910 0.0 6 1870 37 2 1 277 2263 0.0 6 2207 54 2 278 2757 0.0 6 2655 97 5 279 12339 0.0 6 12045 285 4 2 0 1 2 280 2802 0.0 6 2718 79 5 281 40 0.0 6 36 4 282 7 0.0 6 7 283 4 0.0 6 3 0 0 0 1 285 2 0.0 6 2 287 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 603 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 35 0.0 6 33 2 71 3 0.0 6 3 72 3 0.0 6 3 73 3 0.0 6 3 74 6 0.0 6 6 75 1 0.0 6 1 76 1 0.0 6 1 77 7 0.0 6 7 78 5 0.0 6 5 79 5 0.0 6 5 80 5 0.0 6 5 82 4 0.0 6 4 84 1 0.0 6 1 85 7 0.0 6 7 86 1 0.0 6 1 87 1 0.0 6 1 88 3 0.0 6 3 89 1 0.0 6 1 90 3 0.0 6 3 91 6 0.0 6 6 92 1 0.0 6 1 93 1 0.0 6 1 94 4 0.0 6 4 95 3 0.0 6 3 98 2 0.0 6 2 99 2 0.0 6 2 100 1 0.0 6 1 101 3 0.0 6 3 102 2 0.0 6 2 103 2 0.0 6 2 104 2 0.0 6 2 105 1 0.0 6 1 106 5 0.0 6 5 107 3 0.0 6 3 108 2 0.0 6 2 109 4 0.0 6 4 110 3 0.0 6 3 111 1 0.0 6 1 112 2 0.0 6 2 114 7 0.0 6 7 115 3 0.0 6 3 116 2 0.0 6 2 117 3 0.0 6 3 118 1 0.0 6 1 119 1 0.0 6 1 123 1 0.0 6 1 124 2 0.0 6 2 125 1 0.0 6 1 126 9 0.0 6 9 127 4 0.0 6 4 128 4 0.0 6 4 129 2 0.0 6 2 130 3 0.0 6 3 132 4 0.0 6 4 133 5 0.0 6 5 134 1 0.0 6 1 135 1 0.0 6 1 136 2 0.0 6 2 137 4 0.0 6 4 138 3 0.0 6 3 139 3 0.0 6 3 140 4 0.0 6 4 141 2 0.0 6 2 142 2 0.0 6 2 143 1 0.0 6 1 144 2 0.0 6 2 145 3 0.0 6 3 146 4 0.0 6 4 147 2 0.0 6 2 148 4 0.0 6 4 149 3 0.0 6 3 150 4 0.0 6 4 151 6 0.0 6 6 152 3 0.0 6 3 153 3 0.0 6 3 154 1 0.0 6 1 155 2 0.0 6 2 156 5 0.0 6 5 157 1 0.0 6 1 158 4 0.0 6 4 159 3 0.0 6 3 160 8 0.0 6 8 161 3 0.0 6 3 162 4 0.0 6 4 163 7 0.0 6 7 164 4 0.0 6 4 165 1 0.0 6 1 166 3 0.0 6 3 167 5 0.0 6 5 169 3 0.0 6 3 170 3 0.0 6 3 171 2 0.0 6 2 172 1 0.0 6 1 173 11 0.0 6 11 174 5 0.0 6 5 175 3 0.0 6 3 176 11 0.0 6 11 177 2 0.0 6 2 178 5 0.0 6 5 179 1 0.0 6 1 181 2 0.0 6 2 182 4 0.0 6 4 183 6 0.0 6 6 184 3 0.0 6 3 185 4 0.0 6 4 186 3 0.0 6 3 187 6 0.0 6 6 188 10 0.0 6 10 189 4 0.0 6 4 190 3 0.0 6 3 191 3 0.0 6 3 192 9 0.0 6 9 193 8 0.0 6 8 194 2 0.0 6 2 195 4 0.0 6 4 196 7 0.0 6 7 197 2 0.0 6 2 198 3 0.0 6 3 199 5 0.0 6 5 200 6 0.0 6 6 201 8 0.0 6 8 202 2 0.0 6 2 203 4 0.0 6 4 204 1 0.0 6 1 205 3 0.0 6 3 206 3 0.0 6 3 207 1 0.0 6 1 208 2 0.0 6 2 209 3 0.0 6 3 210 6 0.0 6 6 211 3 0.0 6 3 212 2 0.0 6 2 213 2 0.0 6 2 214 3 0.0 6 3 215 3 0.0 6 3 216 2 0.0 6 2 217 7 0.0 6 7 218 2 0.0 6 2 219 2 0.0 6 2 220 2 0.0 6 2 221 3 0.0 6 3 222 1 0.0 6 1 223 8 0.0 6 8 224 5 0.0 6 5 225 1 0.0 6 1 226 3 0.0 6 3 227 2 0.0 6 2 228 2 0.0 6 2 229 2 0.0 6 2 230 4 0.0 6 4 231 2 0.0 6 2 232 2 0.0 6 2 233 1 0.0 6 1 234 1 0.0 6 1 235 4 0.0 6 4 236 4 0.0 6 4 238 1 0.0 6 1 239 2 0.0 6 2 240 3 0.0 6 3 241 2 0.0 6 2 242 3 0.0 6 3 243 3 0.0 6 3 244 4 0.0 6 4 245 1 0.0 6 1 247 4 0.0 6 4 248 2 0.0 6 2 251 4 0.0 6 4 253 4 0.0 6 4 254 1 0.0 6 1 259 3 0.0 6 3 260 1 0.0 6 1 263 1 0.0 6 1 265 2 0.0 6 2 267 1 0.0 6 1 268 1 0.0 6 1 273 1 0.0 6 1 274 1 0.0 6 1 275 1 0.0 6 1 277 1 0.0 6 1 278 2 0.0 6 2 280 1 0.0 6 1 288 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 76023 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 18.7 1 1 11 2 0.0 3 2 12 4 0.0 3 4 13 15 0.0 3 12 3 14 6 0.0 4 6 15 1 0.0 4 1 16 10 0.0 4 9 1 17 17 0.0 5 16 0 1 18 11 0.0 5 11 19 14 0.0 5 13 0 1 20 65 0.0 6 63 2 21 57 0.0 6 54 3 22 31 0.0 6 31 23 30 0.0 6 29 1 24 13 0.0 6 12 1 25 20 0.0 6 19 1 26 14 0.0 6 14 27 13 0.0 6 12 1 28 10 0.0 6 9 0 0 0 0 0 1 29 18 0.0 6 17 0 1 30 16 0.0 6 14 1 1 31 10 0.0 6 10 32 10 0.0 6 10 33 6 0.0 6 6 34 13 0.0 6 13 35 16 0.0 6 16 36 6 0.0 6 6 37 15 0.0 6 14 0 1 38 10 0.0 6 10 39 16 0.0 6 12 3 0 1 40 5 0.0 6 5 41 9 0.0 6 8 1 42 6 0.0 6 5 0 0 1 43 9 0.0 6 8 1 44 25 0.0 6 24 1 45 18 0.0 6 17 1 46 20 0.0 6 19 1 47 24 0.0 6 22 1 0 1 48 9 0.0 6 7 2 49 16 0.0 6 16 50 15 0.0 6 14 1 51 22 0.0 6 20 2 52 21 0.0 6 19 2 53 11 0.0 6 10 1 54 18 0.0 6 18 55 33 0.0 6 28 5 56 23 0.0 6 20 3 57 15 0.0 6 14 1 58 18 0.0 6 15 3 59 17 0.0 6 15 2 60 23 0.0 6 21 2 61 17 0.0 6 15 2 62 19 0.0 6 18 1 63 14 0.0 6 13 1 64 16 0.0 6 15 1 65 18 0.0 6 16 2 66 24 0.0 6 22 2 67 18 0.0 6 17 1 68 34 0.0 6 31 3 69 136 0.0 6 134 2 70 34 0.0 6 32 1 0 1 71 80 0.0 6 76 3 1 72 32 0.0 6 32 73 31 0.0 6 30 1 74 24 0.0 6 22 2 75 29 0.0 6 27 2 76 34 0.0 6 32 2 77 28 0.0 6 28 78 41 0.0 6 41 79 34 0.0 6 32 2 80 24 0.0 6 21 3 81 33 0.0 6 32 0 1 82 43 0.0 6 42 1 83 44 0.0 6 40 4 84 64 0.0 6 59 5 85 54 0.0 6 50 2 0 1 1 86 43 0.0 6 40 3 87 43 0.0 6 39 3 0 1 88 22 0.0 6 20 2 89 53 0.0 6 47 5 0 0 0 0 1 90 45 0.0 6 43 2 91 32 0.0 6 29 3 92 47 0.0 6 45 2 93 76 0.0 6 70 4 0 0 1 0 1 94 98 0.0 6 95 2 1 95 61 0.0 6 57 4 96 56 0.0 6 51 4 1 97 65 0.0 6 61 4 98 68 0.0 6 64 3 1 99 59 0.0 6 55 3 0 0 1 100 65 0.0 6 59 6 101 47 0.0 6 45 2 102 64 0.0 6 63 1 103 99 0.0 6 97 2 104 98 0.0 6 93 4 0 0 0 1 105 81 0.0 6 76 5 106 115 0.0 6 111 4 107 85 0.0 6 81 4 108 76 0.0 6 73 3 109 85 0.0 6 81 4 110 81 0.0 6 76 4 1 111 82 0.0 6 76 6 112 93 0.0 6 90 2 1 113 94 0.0 6 89 4 1 114 119 0.0 6 115 4 115 105 0.0 6 102 3 116 94 0.0 6 90 4 117 92 0.0 6 91 0 0 0 0 1 118 101 0.0 6 100 1 119 121 0.0 6 118 3 120 146 0.0 6 140 6 121 110 0.0 6 105 4 1 122 120 0.0 6 114 5 0 0 0 0 1 123 117 0.0 6 112 4 0 0 0 0 1 124 131 0.0 6 126 4 0 1 125 120 0.0 6 116 3 1 126 130 0.0 6 125 5 127 177 0.0 6 169 6 1 1 128 146 0.0 6 141 4 0 0 0 0 1 129 172 0.0 6 165 7 130 185 0.0 6 178 6 0 0 0 0 1 131 205 0.0 6 200 5 132 207 0.0 6 201 6 133 221 0.0 6 211 9 0 1 134 271 0.0 6 262 8 0 0 0 1 135 373 0.0 6 357 12 3 0 1 136 316 0.0 6 304 11 0 0 0 0 1 137 301 0.0 6 290 7 1 2 0 1 138 246 0.0 6 235 9 0 0 1 0 1 139 291 0.0 6 287 4 140 373 0.0 6 363 9 0 0 1 141 397 0.0 6 389 7 0 0 0 0 1 142 462 0.0 6 446 12 2 0 0 2 143 450 0.0 6 438 10 1 0 1 144 559 0.0 6 545 13 0 0 1 145 431 0.0 6 424 6 0 0 1 146 477 0.0 6 467 9 0 0 0 1 147 422 0.0 6 408 12 0 0 2 148 536 0.0 6 523 11 0 0 0 1 1 149 573 0.0 6 559 13 1 150 635 0.0 6 618 14 1 0 1 0 1 151 611 0.0 6 599 7 4 0 0 1 152 919 0.0 6 898 15 3 0 2 1 153 634 0.0 6 626 7 0 0 1 154 803 0.0 6 778 22 0 0 1 1 1 155 725 0.0 6 709 15 0 1 156 625 0.0 6 608 17 157 720 0.0 6 701 17 0 0 2 158 684 0.0 6 669 10 0 1 4 159 592 0.0 6 576 14 2 160 769 0.0 6 750 18 1 161 515 0.0 6 502 12 0 0 0 1 162 579 0.0 6 565 12 0 0 2 163 631 0.0 6 618 12 0 0 1 164 550 0.0 6 538 10 1 1 165 685 0.0 6 669 13 0 1 1 1 166 701 0.0 6 679 20 2 167 571 0.0 6 550 19 1 1 168 750 0.0 6 739 11 169 424 0.0 6 416 7 0 0 0 1 170 640 0.0 6 626 12 1 0 0 1 171 697 0.0 6 686 10 1 172 478 0.0 6 470 7 1 173 593 0.0 6 575 17 1 174 759 0.0 6 735 23 1 175 1090 0.0 6 1064 26 176 785 0.0 6 764 19 1 1 177 1049 0.0 6 1018 29 1 1 178 806 0.0 6 785 18 2 1 179 731 0.0 6 708 18 2 2 1 180 869 0.0 6 845 23 1 181 674 0.0 6 660 14 182 403 0.0 6 397 5 0 0 0 0 1 183 793 0.0 6 775 18 184 372 0.0 6 365 6 1 185 625 0.0 6 604 21 186 1706 0.0 6 1654 47 1 1 3 187 1542 0.0 6 1502 34 1 1 3 1 188 1336 0.0 6 1287 48 1 189 977 0.0 6 941 34 1 0 1 190 1375 0.0 6 1344 27 1 2 1 191 1119 0.0 6 1097 19 3 192 913 0.0 6 888 23 1 0 1 193 1243 0.0 6 1218 22 3 194 805 0.0 6 773 31 0 0 1 195 1164 0.0 6 1123 40 0 0 1 196 1093 0.0 6 1070 22 0 1 197 684 0.0 6 661 21 0 0 2 198 1622 0.0 6 1578 43 0 0 1 199 1634 0.0 6 1590 44 200 939 0.0 6 907 29 2 0 0 1 201 862 0.0 6 834 27 1 202 708 0.0 6 689 16 2 1 203 629 0.0 6 617 12 204 641 0.0 6 629 11 0 0 0 0 1 205 657 0.0 6 641 15 1 206 276 0.0 6 266 9 1 207 367 0.0 6 356 10 0 0 1 208 662 0.0 6 644 18 209 485 0.0 6 466 17 0 0 2 210 447 0.0 6 436 9 2 211 698 0.0 6 675 21 1 0 1 212 577 0.0 6 562 13 0 1 1 213 501 0.0 6 480 20 1 214 859 0.0 6 832 25 1 1 215 474 0.0 6 463 10 0 0 1 216 737 0.0 6 723 12 2 217 457 0.0 6 439 18 218 489 0.0 6 473 15 1 219 482 0.0 6 467 14 0 0 1 220 369 0.0 6 353 14 2 221 514 0.0 6 501 13 222 326 0.0 6 317 9 223 529 0.0 6 505 20 3 1 224 702 0.0 6 684 18 225 527 0.0 6 513 14 226 410 0.0 6 399 11 227 857 0.0 6 837 20 228 387 0.0 6 372 13 2 229 592 0.0 6 577 15 230 242 0.0 6 237 5 231 314 0.0 6 301 11 0 0 1 0 1 232 286 0.0 6 277 9 233 427 0.0 6 408 17 1 1 234 400 0.0 6 396 4 235 201 0.0 6 197 4 236 118 0.0 6 113 5 237 76 0.0 6 74 2 238 70 0.0 6 68 2 239 72 0.0 6 68 4 240 96 0.0 6 95 1 241 101 0.0 6 97 4 242 138 0.0 6 136 2 243 241 0.0 6 233 8 244 154 0.0 6 150 3 0 0 1 245 95 0.0 6 92 3 246 102 0.0 6 100 2 247 63 0.0 6 63 248 88 0.0 6 86 2 249 186 0.0 6 181 5 250 88 0.0 6 83 5 251 59 0.0 6 59 252 83 0.0 6 80 3 253 64 0.0 6 61 3 254 84 0.0 6 83 1 255 62 0.0 6 61 0 0 0 0 1 256 14 0.0 6 14 257 5 0.0 6 5 258 2 0.0 6 2 259 3 0.0 6 3 264 1 0.0 6 1 269 2 0.0 6 2 271 1 0.0 6 1 278 3 0.0 6 3 279 4 0.0 6 4 280 54 0.0 6 51 3 281 181 0.0 6 177 4 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 400 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 59 2 0.0 6 2 70 2 0.0 6 2 71 6 0.0 6 6 72 2 0.0 6 2 73 2 0.0 6 2 74 4 0.0 6 4 75 3 0.0 6 3 76 3 0.0 6 3 77 1 0.0 6 1 78 3 0.0 6 3 79 3 0.0 6 3 80 2 0.0 6 2 81 2 0.0 6 2 82 1 0.0 6 1 83 4 0.0 6 4 84 1 0.0 6 1 85 2 0.0 6 2 86 3 0.0 6 3 87 3 0.0 6 3 88 1 0.0 6 1 89 2 0.0 6 2 90 7 0.0 6 6 0 0 0 1 91 5 0.0 6 5 92 2 0.0 6 2 93 3 0.0 6 3 94 3 0.0 6 3 95 5 0.0 6 5 96 1 0.0 6 1 97 4 0.0 6 4 98 1 0.0 6 1 99 4 0.0 6 4 101 2 0.0 6 2 102 4 0.0 6 4 103 6 0.0 6 6 104 1 0.0 6 1 105 2 0.0 6 2 106 2 0.0 6 2 107 5 0.0 6 5 108 11 0.0 6 11 109 2 0.0 6 2 110 5 0.0 6 5 111 1 0.0 6 1 112 2 0.0 6 2 113 4 0.0 6 4 114 4 0.0 6 4 115 1 0.0 6 1 116 3 0.0 6 3 118 4 0.0 6 4 119 2 0.0 6 2 120 2 0.0 6 2 122 1 0.0 6 1 123 5 0.0 6 5 124 3 0.0 6 3 125 3 0.0 6 3 126 2 0.0 6 2 127 1 0.0 6 1 129 3 0.0 6 3 130 4 0.0 6 4 131 2 0.0 6 2 132 7 0.0 6 7 133 1 0.0 6 1 134 1 0.0 6 1 135 9 0.0 6 9 136 4 0.0 6 4 137 2 0.0 6 2 138 7 0.0 6 7 139 3 0.0 6 3 140 3 0.0 6 3 141 1 0.0 6 1 143 5 0.0 6 5 144 5 0.0 6 5 145 3 0.0 6 3 146 2 0.0 6 2 147 3 0.0 6 3 148 2 0.0 6 2 149 4 0.0 6 3 0 0 0 1 150 3 0.0 6 3 151 3 0.0 6 3 152 2 0.0 6 2 153 1 0.0 6 1 154 2 0.0 6 2 155 2 0.0 6 2 156 3 0.0 6 3 157 3 0.0 6 3 158 5 0.0 6 5 159 6 0.0 6 6 160 4 0.0 6 4 161 5 0.0 6 5 162 3 0.0 6 3 163 3 0.0 6 3 164 2 0.0 6 2 165 5 0.0 6 5 166 5 0.0 6 5 167 3 0.0 6 3 168 4 0.0 6 4 169 3 0.0 6 3 170 1 0.0 6 1 172 4 0.0 6 4 173 6 0.0 6 6 174 6 0.0 6 6 175 5 0.0 6 5 176 1 0.0 6 1 177 3 0.0 6 3 178 1 0.0 6 1 180 3 0.0 6 3 181 2 0.0 6 2 182 2 0.0 6 2 183 2 0.0 6 2 184 2 0.0 6 2 185 2 0.0 6 2 186 5 0.0 6 5 187 5 0.0 6 4 1 189 2 0.0 6 2 190 5 0.0 6 5 191 2 0.0 6 2 192 3 0.0 6 3 193 3 0.0 6 3 194 1 0.0 6 1 195 2 0.0 6 2 196 1 0.0 6 1 197 1 0.0 6 1 198 1 0.0 6 1 199 2 0.0 6 1 0 1 200 1 0.0 6 1 201 5 0.0 6 5 202 2 0.0 6 2 203 1 0.0 6 1 204 2 0.0 6 2 205 1 0.0 6 1 206 1 0.0 6 1 209 1 0.0 6 1 213 2 0.0 6 2 214 1 0.0 6 1 216 1 0.0 6 1 221 1 0.0 6 1 225 1 0.0 6 1 226 1 0.0 6 1 230 1 0.0 6 1 234 1 0.0 6 1 235 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_06_101_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_06_328_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_06_101_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_06_328_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 9.55 s (168 µs/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 56,706 Read 1 with adapter: 56,702 (100.0%) Read 2 with adapter: 56,676 (99.9%) == Read fate breakdown == Pairs that were too short: 34 (0.1%) Pairs discarded as untrimmed: 34 (0.1%) Pairs written (passing filters): 56,638 (99.9%) Total basepairs processed: 24,338,152 bp Read 1: 13,704,552 bp Read 2: 10,633,600 bp Total written (filtered): 3,486,875 bp (14.3%) Read 1: 1,812,815 bp Read 2: 1,674,060 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 55439 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 886.0 0 0 2 4 2 221.5 1 1 1 6 1 13.8 1 0 0 1 7 1 3.5 2 1 8 1 0.9 2 1 9 2 0.2 2 1 1 10 1 0.1 3 1 12 1 0.0 3 1 13 1 0.0 3 1 14 1 0.0 4 1 15 2 0.0 4 2 16 4 0.0 4 2 2 17 4 0.0 5 3 0 1 19 3 0.0 5 2 1 20 74 0.0 6 73 1 21 45 0.0 6 44 1 22 42 0.0 6 40 2 23 24 0.0 6 23 1 24 15 0.0 6 14 1 25 17 0.0 6 17 26 8 0.0 6 7 1 27 9 0.0 6 9 28 10 0.0 6 10 29 12 0.0 6 12 30 5 0.0 6 5 31 5 0.0 6 5 32 6 0.0 6 6 33 5 0.0 6 4 1 34 5 0.0 6 5 35 3 0.0 6 3 36 1 0.0 6 1 37 3 0.0 6 3 38 4 0.0 6 3 1 39 5 0.0 6 5 40 1 0.0 6 1 41 8 0.0 6 7 1 42 5 0.0 6 4 1 43 7 0.0 6 7 44 7 0.0 6 7 45 3 0.0 6 2 1 46 5 0.0 6 5 47 3 0.0 6 3 48 2 0.0 6 1 1 49 5 0.0 6 5 50 7 0.0 6 7 51 8 0.0 6 8 52 2 0.0 6 2 53 7 0.0 6 7 54 7 0.0 6 7 55 4 0.0 6 4 56 6 0.0 6 5 1 57 4 0.0 6 4 58 3 0.0 6 2 1 59 5 0.0 6 5 60 6 0.0 6 6 61 4 0.0 6 4 62 3 0.0 6 3 63 4 0.0 6 4 64 3 0.0 6 3 65 6 0.0 6 6 66 7 0.0 6 7 67 4 0.0 6 4 68 2 0.0 6 2 69 3 0.0 6 3 70 5 0.0 6 5 71 13 0.0 6 13 72 6 0.0 6 5 1 73 7 0.0 6 6 1 74 6 0.0 6 5 1 75 9 0.0 6 9 76 10 0.0 6 10 77 8 0.0 6 8 78 4 0.0 6 4 79 5 0.0 6 5 80 4 0.0 6 4 81 10 0.0 6 9 1 82 6 0.0 6 5 1 83 9 0.0 6 8 1 84 5 0.0 6 5 85 12 0.0 6 12 86 14 0.0 6 14 87 6 0.0 6 5 1 88 9 0.0 6 9 89 11 0.0 6 10 1 90 6 0.0 6 6 91 6 0.0 6 6 92 8 0.0 6 8 93 6 0.0 6 6 94 5 0.0 6 5 95 3 0.0 6 3 96 11 0.0 6 11 97 4 0.0 6 4 98 10 0.0 6 10 99 4 0.0 6 3 1 100 9 0.0 6 9 101 12 0.0 6 10 2 102 18 0.0 6 16 2 103 9 0.0 6 9 104 13 0.0 6 12 1 105 18 0.0 6 18 106 23 0.0 6 20 3 107 13 0.0 6 12 1 108 20 0.0 6 20 109 10 0.0 6 10 110 10 0.0 6 9 0 0 0 1 111 20 0.0 6 20 112 20 0.0 6 19 1 113 14 0.0 6 14 114 17 0.0 6 17 115 20 0.0 6 20 116 21 0.0 6 20 1 117 20 0.0 6 20 118 17 0.0 6 16 1 119 8 0.0 6 8 120 21 0.0 6 20 0 0 1 121 20 0.0 6 19 1 122 13 0.0 6 13 123 14 0.0 6 13 1 124 19 0.0 6 18 1 125 16 0.0 6 16 126 49 0.0 6 49 127 27 0.0 6 26 1 128 20 0.0 6 20 129 27 0.0 6 27 130 23 0.0 6 23 131 22 0.0 6 21 1 132 19 0.0 6 19 133 22 0.0 6 22 134 24 0.0 6 22 2 135 34 0.0 6 34 136 32 0.0 6 31 1 137 26 0.0 6 26 138 36 0.0 6 36 139 58 0.0 6 57 0 0 1 140 141 0.0 6 139 1 0 1 141 282 0.0 6 282 142 398 0.0 6 397 1 143 308 0.0 6 308 144 260 0.0 6 260 145 422 0.0 6 421 1 146 397 0.0 6 396 1 147 409 0.0 6 408 1 148 393 0.0 6 393 149 463 0.0 6 463 150 543 0.0 6 541 2 151 353 0.0 6 353 152 271 0.0 6 269 1 0 1 153 250 0.0 6 248 2 154 270 0.0 6 268 1 1 155 237 0.0 6 237 156 214 0.0 6 211 3 157 246 0.0 6 246 158 221 0.0 6 218 2 0 1 159 240 0.0 6 239 1 160 276 0.0 6 274 1 0 1 161 257 0.0 6 255 2 162 267 0.0 6 265 2 163 276 0.0 6 275 1 164 211 0.0 6 211 165 240 0.0 6 239 1 166 218 0.0 6 215 3 167 192 0.0 6 191 1 168 224 0.0 6 223 1 169 233 0.0 6 231 2 170 201 0.0 6 200 1 171 195 0.0 6 195 172 273 0.0 6 272 1 173 278 0.0 6 274 4 174 211 0.0 6 210 0 0 0 0 1 175 253 0.0 6 250 3 176 263 0.0 6 259 4 177 278 0.0 6 276 2 178 443 0.0 6 443 179 244 0.0 6 241 3 180 196 0.0 6 196 181 230 0.0 6 230 182 213 0.0 6 211 1 1 183 241 0.0 6 238 3 184 292 0.0 6 290 2 185 272 0.0 6 271 1 186 245 0.0 6 243 2 187 298 0.0 6 294 3 0 0 1 188 222 0.0 6 222 189 257 0.0 6 253 2 0 1 0 1 190 280 0.0 6 278 1 1 191 292 0.0 6 288 3 0 0 0 0 1 192 330 0.0 6 328 1 0 1 193 364 0.0 6 361 3 194 347 0.0 6 340 7 195 393 0.0 6 389 3 0 1 196 495 0.0 6 491 3 0 0 1 197 481 0.0 6 474 7 198 569 0.0 6 561 7 1 199 592 0.0 6 589 3 200 470 0.0 6 462 8 201 441 0.0 6 440 1 202 540 0.0 6 539 1 203 424 0.0 6 423 1 204 466 0.0 6 464 2 205 459 0.0 6 454 4 0 0 0 1 206 460 0.0 6 454 5 0 1 207 677 0.0 6 670 7 208 564 0.0 6 558 6 209 537 0.0 6 532 3 0 0 1 0 1 210 439 0.0 6 431 8 211 410 0.0 6 408 2 212 566 0.0 6 562 4 213 592 0.0 6 588 3 0 0 0 0 1 214 586 0.0 6 583 3 215 688 0.0 6 682 5 0 0 0 1 216 797 0.0 6 791 4 2 217 671 0.0 6 664 7 218 644 0.0 6 638 6 219 665 0.0 6 662 3 220 611 0.0 6 605 5 0 1 221 515 0.0 6 513 2 222 477 0.0 6 473 3 1 223 510 0.0 6 506 4 224 551 0.0 6 549 2 225 623 0.0 6 619 4 226 612 0.0 6 607 5 227 615 0.0 6 611 4 228 537 0.0 6 530 5 2 229 525 0.0 6 525 230 560 0.0 6 554 5 0 1 231 529 0.0 6 526 3 232 525 0.0 6 522 2 0 0 1 233 540 0.0 6 538 2 234 572 0.0 6 569 3 235 506 0.0 6 503 2 0 0 1 236 407 0.0 6 406 1 237 492 0.0 6 487 5 238 559 0.0 6 558 1 239 417 0.0 6 414 3 240 451 0.0 6 448 2 0 1 241 456 0.0 6 449 7 242 455 0.0 6 451 4 243 431 0.0 6 428 3 244 433 0.0 6 432 1 245 343 0.0 6 340 3 246 362 0.0 6 360 2 247 330 0.0 6 328 2 248 317 0.0 6 316 0 1 249 276 0.0 6 274 1 0 0 1 250 303 0.0 6 302 1 251 331 0.0 6 327 4 252 303 0.0 6 303 253 266 0.0 6 262 2 0 0 0 1 1 254 291 0.0 6 289 2 255 264 0.0 6 261 3 256 268 0.0 6 267 1 257 274 0.0 6 272 2 258 231 0.0 6 228 3 259 263 0.0 6 263 260 330 0.0 6 330 261 261 0.0 6 258 3 262 188 0.0 6 188 263 212 0.0 6 211 1 264 183 0.0 6 181 2 265 197 0.0 6 196 1 266 175 0.0 6 174 1 267 208 0.0 6 208 268 160 0.0 6 160 269 196 0.0 6 194 2 270 210 0.0 6 208 2 271 178 0.0 6 178 272 138 0.0 6 138 273 172 0.0 6 172 274 221 0.0 6 219 2 275 210 0.0 6 208 2 276 264 0.0 6 262 2 277 311 0.0 6 307 4 278 380 0.0 6 379 1 279 1692 0.0 6 1674 16 2 280 1623 0.0 6 1619 3 0 0 0 1 281 20 0.0 6 19 1 282 1 0.0 6 1 283 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1263 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 30 0.0 6 29 1 62 78 0.0 6 77 1 69 1 0.0 6 1 70 1 0.0 6 1 71 5 0.0 6 5 72 12 0.0 6 12 73 7 0.0 6 7 74 9 0.0 6 9 75 10 0.0 6 10 76 9 0.0 6 9 77 12 0.0 6 12 78 10 0.0 6 10 79 13 0.0 6 13 80 9 0.0 6 9 81 15 0.0 6 15 82 10 0.0 6 10 83 9 0.0 6 9 84 1 0.0 6 1 85 11 0.0 6 11 86 11 0.0 6 10 1 87 5 0.0 6 5 88 10 0.0 6 10 89 6 0.0 6 6 90 10 0.0 6 10 91 9 0.0 6 9 92 11 0.0 6 11 93 10 0.0 6 10 94 4 0.0 6 4 95 5 0.0 6 5 96 4 0.0 6 4 97 5 0.0 6 5 98 2 0.0 6 2 99 8 0.0 6 8 100 2 0.0 6 2 101 3 0.0 6 3 102 3 0.0 6 3 103 2 0.0 6 2 104 4 0.0 6 4 105 3 0.0 6 3 106 6 0.0 6 6 107 5 0.0 6 5 108 12 0.0 6 12 109 8 0.0 6 8 110 9 0.0 6 9 112 1 0.0 6 1 113 8 0.0 6 8 114 2 0.0 6 2 115 5 0.0 6 5 116 3 0.0 6 3 117 3 0.0 6 3 118 3 0.0 6 3 119 6 0.0 6 6 120 6 0.0 6 6 121 2 0.0 6 2 122 6 0.0 6 6 123 4 0.0 6 4 124 6 0.0 6 6 125 5 0.0 6 5 126 4 0.0 6 4 127 5 0.0 6 5 128 3 0.0 6 3 129 3 0.0 6 3 130 4 0.0 6 4 131 1 0.0 6 1 132 8 0.0 6 8 133 3 0.0 6 3 134 2 0.0 6 2 135 4 0.0 6 4 136 7 0.0 6 7 137 4 0.0 6 4 138 6 0.0 6 6 139 1 0.0 6 1 140 1 0.0 6 1 141 2 0.0 6 2 142 4 0.0 6 4 143 5 0.0 6 5 144 10 0.0 6 10 145 1 0.0 6 1 146 4 0.0 6 4 147 6 0.0 6 6 148 9 0.0 6 9 149 7 0.0 6 7 150 3 0.0 6 3 151 7 0.0 6 7 152 4 0.0 6 4 153 7 0.0 6 7 154 9 0.0 6 9 155 6 0.0 6 6 156 5 0.0 6 5 157 7 0.0 6 7 158 10 0.0 6 10 159 4 0.0 6 4 160 4 0.0 6 4 161 3 0.0 6 3 162 8 0.0 6 8 163 5 0.0 6 5 164 7 0.0 6 7 165 3 0.0 6 3 166 7 0.0 6 7 167 6 0.0 6 6 168 8 0.0 6 8 169 6 0.0 6 6 170 5 0.0 6 5 171 3 0.0 6 3 172 11 0.0 6 11 173 6 0.0 6 5 1 174 5 0.0 6 5 175 6 0.0 6 6 176 6 0.0 6 6 177 8 0.0 6 8 178 7 0.0 6 7 179 3 0.0 6 3 180 8 0.0 6 8 181 6 0.0 6 6 182 6 0.0 6 6 183 6 0.0 6 6 184 5 0.0 6 5 185 3 0.0 6 3 186 3 0.0 6 3 187 8 0.0 6 8 188 4 0.0 6 4 189 9 0.0 6 9 190 10 0.0 6 10 191 10 0.0 6 10 192 10 0.0 6 10 193 8 0.0 6 8 194 5 0.0 6 5 195 6 0.0 6 6 196 5 0.0 6 5 197 5 0.0 6 5 198 4 0.0 6 4 199 10 0.0 6 10 200 5 0.0 6 5 201 14 0.0 6 14 202 15 0.0 6 15 203 8 0.0 6 8 204 8 0.0 6 8 205 11 0.0 6 11 206 2 0.0 6 2 207 5 0.0 6 5 208 7 0.0 6 7 210 6 0.0 6 6 211 11 0.0 6 11 212 9 0.0 6 9 213 4 0.0 6 4 214 3 0.0 6 3 215 5 0.0 6 5 217 6 0.0 6 6 218 5 0.0 6 5 219 5 0.0 6 5 220 7 0.0 6 7 221 3 0.0 6 3 222 7 0.0 6 7 223 4 0.0 6 4 224 8 0.0 6 8 225 4 0.0 6 4 226 7 0.0 6 7 227 6 0.0 6 6 228 3 0.0 6 3 229 2 0.0 6 2 230 9 0.0 6 9 231 6 0.0 6 6 232 3 0.0 6 3 233 7 0.0 6 7 234 5 0.0 6 5 235 3 0.0 6 3 236 4 0.0 6 4 237 8 0.0 6 8 238 7 0.0 6 7 239 8 0.0 6 8 240 5 0.0 6 5 241 2 0.0 6 2 242 6 0.0 6 6 243 2 0.0 6 2 244 4 0.0 6 4 245 6 0.0 6 6 246 10 0.0 6 10 247 8 0.0 6 8 248 2 0.0 6 2 249 3 0.0 6 3 250 2 0.0 6 2 251 3 0.0 6 2 1 252 6 0.0 6 6 253 2 0.0 6 2 254 2 0.0 6 2 255 1 0.0 6 1 256 5 0.0 6 5 258 6 0.0 6 6 259 3 0.0 6 3 260 3 0.0 6 3 261 5 0.0 6 5 262 6 0.0 6 6 263 3 0.0 6 3 264 4 0.0 6 4 265 2 0.0 6 2 266 1 0.0 6 1 268 1 0.0 6 1 269 2 0.0 6 2 270 1 0.0 6 1 271 2 0.0 6 2 272 1 0.0 6 1 273 1 0.0 6 1 274 2 0.0 6 2 275 3 0.0 6 3 277 1 0.0 6 1 278 1 0.0 6 1 279 1 0.0 6 1 280 1 0.0 6 1 281 4 0.0 6 4 282 6 0.0 6 6 283 2 0.0 6 2 286 2 0.0 6 2 288 1 0.0 6 1 289 1 0.0 6 1 292 1 0.0 6 1 293 1 0.0 6 1 294 2 0.0 6 2 300 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 55762 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 886.0 0 1 4 2 221.5 1 1 1 7 1 3.5 2 0 0 1 9 1 0.2 2 1 12 3 0.0 3 2 0 0 1 13 2 0.0 3 2 14 4 0.0 4 3 1 15 3 0.0 4 3 16 5 0.0 4 4 0 1 17 24 0.0 5 22 2 18 5 0.0 5 3 1 1 19 44 0.0 5 40 4 20 79 0.0 6 76 3 21 41 0.0 6 41 22 31 0.0 6 31 23 20 0.0 6 19 1 24 20 0.0 6 18 1 1 25 17 0.0 6 17 26 10 0.0 6 9 1 27 11 0.0 6 11 28 10 0.0 6 10 29 15 0.0 6 14 1 30 8 0.0 6 6 2 31 23 0.0 6 18 2 1 2 32 16 0.0 6 13 3 33 20 0.0 6 18 1 0 1 34 15 0.0 6 13 2 35 15 0.0 6 10 5 36 8 0.0 6 7 1 37 13 0.0 6 13 38 11 0.0 6 11 39 13 0.0 6 11 2 40 16 0.0 6 13 2 0 0 0 0 1 41 10 0.0 6 9 1 42 11 0.0 6 10 1 43 18 0.0 6 17 1 44 8 0.0 6 8 45 13 0.0 6 11 1 0 0 0 0 1 46 10 0.0 6 8 2 47 17 0.0 6 14 3 48 17 0.0 6 17 49 19 0.0 6 19 50 23 0.0 6 21 1 0 0 1 51 26 0.0 6 22 1 0 3 52 13 0.0 6 13 53 24 0.0 6 23 1 54 30 0.0 6 26 3 0 0 0 1 55 23 0.0 6 22 1 56 25 0.0 6 25 57 15 0.0 6 15 58 18 0.0 6 16 1 1 59 20 0.0 6 20 60 27 0.0 6 27 61 32 0.0 6 31 1 62 14 0.0 6 13 1 63 21 0.0 6 20 1 64 16 0.0 6 13 2 1 65 22 0.0 6 20 2 66 23 0.0 6 22 1 67 34 0.0 6 33 1 68 28 0.0 6 26 2 69 45 0.0 6 42 2 1 70 113 0.0 6 104 5 2 1 0 1 71 54 0.0 6 53 1 72 52 0.0 6 50 1 0 0 0 1 73 41 0.0 6 40 1 74 58 0.0 6 53 4 0 0 0 0 1 75 34 0.0 6 33 0 0 0 1 76 30 0.0 6 28 1 1 77 35 0.0 6 32 3 78 29 0.0 6 27 2 79 30 0.0 6 29 0 0 0 1 80 31 0.0 6 28 3 81 41 0.0 6 36 4 0 1 82 47 0.0 6 47 83 42 0.0 6 36 4 0 0 0 2 84 55 0.0 6 52 1 1 0 0 1 85 49 0.0 6 47 2 86 80 0.0 6 76 2 1 1 87 40 0.0 6 37 3 88 51 0.0 6 48 2 0 1 89 54 0.0 6 51 2 1 90 42 0.0 6 42 91 47 0.0 6 47 92 44 0.0 6 43 0 0 0 0 1 93 70 0.0 6 64 4 0 0 1 0 1 94 60 0.0 6 57 3 95 66 0.0 6 61 4 0 0 0 1 96 65 0.0 6 62 2 1 97 78 0.0 6 76 2 98 54 0.0 6 53 0 0 0 1 99 75 0.0 6 71 1 3 100 66 0.0 6 62 3 0 1 101 75 0.0 6 72 1 1 0 0 0 1 102 70 0.0 6 68 1 1 103 76 0.0 6 71 4 1 104 75 0.0 6 72 3 105 95 0.0 6 92 3 106 113 0.0 6 104 3 1 0 1 2 2 107 162 0.0 6 154 5 0 0 2 1 108 88 0.0 6 85 3 109 89 0.0 6 87 1 1 110 102 0.0 6 95 4 0 2 0 1 111 77 0.0 6 76 0 1 112 98 0.0 6 92 5 0 0 1 113 121 0.0 6 115 5 0 1 114 121 0.0 6 116 3 1 0 1 115 141 0.0 6 137 3 1 116 117 0.0 6 114 3 117 112 0.0 6 109 2 0 0 1 118 156 0.0 6 151 4 0 0 0 0 1 119 110 0.0 6 109 0 1 120 130 0.0 6 128 2 121 177 0.0 6 173 3 0 0 0 0 1 122 148 0.0 6 147 1 123 155 0.0 6 153 2 124 163 0.0 6 157 5 1 125 198 0.0 6 192 5 0 0 0 1 126 195 0.0 6 191 4 127 214 0.0 6 209 4 0 1 128 261 0.0 6 255 5 1 129 233 0.0 6 226 6 0 0 1 130 301 0.0 6 285 10 1 3 2 131 269 0.0 6 263 5 1 132 285 0.0 6 280 4 1 133 309 0.0 6 306 2 0 1 134 326 0.0 6 320 4 0 0 0 1 1 135 372 0.0 6 365 7 136 386 0.0 6 377 7 0 0 2 137 415 0.0 6 409 5 0 0 1 138 466 0.0 6 463 2 0 0 1 139 493 0.0 6 488 4 0 0 1 140 572 0.0 6 560 12 141 629 0.0 6 622 6 1 142 623 0.0 6 613 8 1 0 0 1 143 652 0.0 6 643 8 1 144 793 0.0 6 781 10 0 2 145 818 0.0 6 803 11 1 0 0 1 2 146 789 0.0 6 776 10 1 1 1 147 836 0.0 6 825 9 0 1 1 148 882 0.0 6 868 10 0 0 3 1 149 962 0.0 6 948 11 0 1 1 1 150 888 0.0 6 879 9 151 976 0.0 6 958 14 1 0 2 1 152 968 0.0 6 944 18 1 0 1 2 2 153 1252 0.0 6 1232 16 1 0 3 154 1011 0.0 6 992 14 0 1 2 2 155 1110 0.0 6 1095 12 2 0 1 156 1115 0.0 6 1098 14 0 1 0 1 1 157 910 0.0 6 888 19 0 0 1 2 158 983 0.0 6 960 20 0 0 1 0 2 159 901 0.0 6 881 16 0 1 1 1 1 160 983 0.0 6 972 8 0 0 2 0 1 161 922 0.0 6 911 9 1 0 0 1 162 821 0.0 6 809 9 2 1 163 839 0.0 6 821 16 0 1 1 164 775 0.0 6 768 4 1 0 0 0 2 165 700 0.0 6 690 8 1 0 0 0 1 166 741 0.0 6 727 12 1 1 167 689 0.0 6 682 5 2 168 635 0.0 6 624 8 0 1 0 1 1 169 762 0.0 6 749 13 170 661 0.0 6 646 14 0 1 171 803 0.0 6 797 6 172 714 0.0 6 705 7 0 1 1 173 695 0.0 6 684 11 174 803 0.0 6 792 8 1 0 1 0 1 175 822 0.0 6 812 8 1 0 0 0 1 176 753 0.0 6 743 8 0 1 0 0 1 177 756 0.0 6 750 6 178 620 0.0 6 613 4 1 1 0 1 179 661 0.0 6 655 5 1 180 554 0.0 6 547 6 1 181 560 0.0 6 553 5 1 1 182 452 0.0 6 447 5 183 440 0.0 6 434 6 184 418 0.0 6 414 3 1 185 429 0.0 6 423 3 2 1 186 615 0.0 6 611 3 0 0 1 187 734 0.0 6 723 9 0 1 0 0 1 188 666 0.0 6 659 6 1 189 480 0.0 6 472 7 1 190 491 0.0 6 489 2 191 416 0.0 6 410 5 0 0 1 192 369 0.0 6 365 4 193 340 0.0 6 337 3 194 376 0.0 6 369 5 1 0 0 1 195 300 0.0 6 292 7 0 0 0 0 1 196 373 0.0 6 369 4 197 247 0.0 6 243 2 2 198 259 0.0 6 255 4 199 383 0.0 6 377 6 200 263 0.0 6 259 3 1 201 278 0.0 6 271 6 1 202 239 0.0 6 238 1 203 223 0.0 6 221 1 0 1 204 187 0.0 6 182 4 0 1 205 187 0.0 6 183 4 206 142 0.0 6 142 207 120 0.0 6 120 208 150 0.0 6 149 1 209 143 0.0 6 141 2 210 120 0.0 6 118 2 211 100 0.0 6 100 212 119 0.0 6 119 213 70 0.0 6 69 0 0 1 214 83 0.0 6 81 2 215 96 0.0 6 93 3 216 116 0.0 6 115 1 217 125 0.0 6 125 218 67 0.0 6 66 1 219 70 0.0 6 67 2 1 220 69 0.0 6 69 221 72 0.0 6 72 222 50 0.0 6 49 1 223 54 0.0 6 54 224 68 0.0 6 67 1 225 57 0.0 6 57 226 45 0.0 6 45 227 44 0.0 6 43 0 0 1 228 41 0.0 6 40 0 1 229 58 0.0 6 55 1 1 1 230 29 0.0 6 29 231 25 0.0 6 24 1 232 25 0.0 6 25 233 30 0.0 6 30 234 21 0.0 6 21 235 10 0.0 6 10 236 11 0.0 6 11 237 12 0.0 6 12 238 6 0.0 6 6 239 9 0.0 6 9 240 9 0.0 6 9 241 9 0.0 6 9 242 10 0.0 6 9 0 0 1 243 6 0.0 6 6 244 14 0.0 6 14 245 5 0.0 6 5 246 6 0.0 6 6 247 1 0.0 6 1 248 4 0.0 6 4 249 4 0.0 6 4 250 5 0.0 6 5 254 1 0.0 6 1 280 1 0.0 6 1 281 13 0.0 6 13 282 77 0.0 6 73 2 1 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 914 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 54 1 0.0 6 1 55 34 0.0 6 34 64 1 0.0 6 1 69 4 0.0 6 4 70 9 0.0 6 9 71 9 0.0 6 9 72 10 0.0 6 10 73 11 0.0 6 10 1 74 16 0.0 6 16 75 22 0.0 6 22 76 13 0.0 6 13 77 18 0.0 6 18 78 16 0.0 6 16 79 20 0.0 6 20 80 9 0.0 6 9 81 12 0.0 6 12 82 9 0.0 6 9 83 8 0.0 6 7 0 0 0 1 84 7 0.0 6 7 85 11 0.0 6 11 86 8 0.0 6 8 87 8 0.0 6 8 88 3 0.0 6 3 89 11 0.0 6 10 0 0 1 90 10 0.0 6 10 91 13 0.0 6 12 1 92 8 0.0 6 8 93 10 0.0 6 10 94 4 0.0 6 4 95 9 0.0 6 9 96 8 0.0 6 8 97 2 0.0 6 2 98 9 0.0 6 8 1 99 2 0.0 6 2 100 2 0.0 6 2 101 3 0.0 6 3 102 10 0.0 6 10 103 6 0.0 6 6 104 7 0.0 6 7 105 2 0.0 6 2 106 7 0.0 6 7 107 7 0.0 6 7 108 4 0.0 6 4 109 3 0.0 6 3 110 6 0.0 6 6 111 7 0.0 6 7 112 4 0.0 6 4 113 2 0.0 6 2 114 5 0.0 6 4 0 0 1 115 2 0.0 6 2 116 5 0.0 6 5 117 7 0.0 6 7 118 4 0.0 6 4 119 2 0.0 6 2 120 5 0.0 6 5 121 6 0.0 6 6 122 5 0.0 6 5 123 6 0.0 6 6 124 6 0.0 6 6 125 8 0.0 6 8 126 9 0.0 6 9 127 3 0.0 6 3 128 2 0.0 6 2 129 2 0.0 6 2 130 9 0.0 6 9 131 5 0.0 6 5 132 2 0.0 6 2 133 7 0.0 6 7 134 2 0.0 6 2 135 3 0.0 6 3 136 7 0.0 6 7 137 6 0.0 6 6 138 2 0.0 6 2 139 3 0.0 6 3 140 10 0.0 6 10 141 5 0.0 6 5 142 3 0.0 6 3 143 2 0.0 6 2 144 3 0.0 6 3 145 2 0.0 6 2 146 4 0.0 6 4 147 8 0.0 6 8 148 2 0.0 6 2 149 6 0.0 6 6 150 4 0.0 6 4 151 6 0.0 6 6 152 4 0.0 6 4 153 7 0.0 6 7 154 4 0.0 6 4 155 5 0.0 6 5 156 2 0.0 6 2 157 5 0.0 6 5 158 8 0.0 6 8 159 6 0.0 6 6 160 7 0.0 6 7 161 6 0.0 6 6 162 6 0.0 6 6 163 4 0.0 6 4 164 8 0.0 6 8 165 8 0.0 6 8 166 3 0.0 6 3 167 8 0.0 6 8 168 7 0.0 6 7 169 5 0.0 6 5 170 6 0.0 6 6 171 8 0.0 6 8 172 6 0.0 6 6 173 8 0.0 6 8 174 4 0.0 6 4 175 4 0.0 6 3 0 0 0 1 176 9 0.0 6 9 177 9 0.0 6 8 1 178 7 0.0 6 7 179 2 0.0 6 2 180 2 0.0 6 2 181 9 0.0 6 9 182 2 0.0 6 2 184 6 0.0 6 6 185 7 0.0 6 7 186 4 0.0 6 4 187 5 0.0 6 5 188 4 0.0 6 4 189 6 0.0 6 6 190 7 0.0 6 7 191 4 0.0 6 4 192 2 0.0 6 2 193 4 0.0 6 4 194 2 0.0 6 2 195 6 0.0 6 6 196 4 0.0 6 4 197 4 0.0 6 4 198 3 0.0 6 3 199 4 0.0 6 4 200 10 0.0 6 10 202 4 0.0 6 4 203 7 0.0 6 7 204 2 0.0 6 2 205 2 0.0 6 2 206 4 0.0 6 4 208 2 0.0 6 2 209 1 0.0 6 1 210 2 0.0 6 2 211 3 0.0 6 3 212 4 0.0 6 4 213 1 0.0 6 1 214 2 0.0 6 2 215 4 0.0 6 4 216 2 0.0 6 2 218 1 0.0 6 1 219 2 0.0 6 1 0 0 1 222 4 0.0 6 4 223 1 0.0 6 1 225 3 0.0 6 3 227 1 0.0 6 1 228 2 0.0 6 2 230 1 0.0 6 1 232 1 0.0 6 1 235 1 0.0 6 1 237 1 0.0 6 1 243 1 0.0 6 1 259 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_15_102_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_15_329_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_15_102_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_15_329_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 7.84 s (72 µs/read; 0.83 M reads/minute). === Summary === Total read pairs processed: 108,436 Read 1 with adapter: 108,425 (100.0%) Read 2 with adapter: 108,374 (99.9%) == Read fate breakdown == Pairs that were too short: 28 (0.0%) Pairs discarded as untrimmed: 71 (0.1%) Pairs written (passing filters): 108,337 (99.9%) Total basepairs processed: 47,629,773 bp Read 1: 25,791,004 bp Read 2: 21,838,769 bp Total written (filtered): 7,173,110 bp (15.1%) Read 1: 3,561,866 bp Read 2: 3,611,244 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 105147 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 1694.3 0 3 4 3 423.6 1 2 1 5 1 105.9 1 0 0 1 6 2 26.5 1 0 0 2 7 4 6.6 2 2 0 2 8 1 1.7 2 1 9 1 0.4 2 1 10 2 0.1 3 1 1 14 2 0.0 4 2 15 1 0.0 4 1 16 3 0.0 4 2 1 17 3 0.0 5 3 19 2 0.0 5 2 20 181 0.0 6 179 2 21 83 0.0 6 78 5 22 118 0.0 6 113 4 1 23 57 0.0 6 55 2 24 74 0.0 6 71 3 25 31 0.0 6 29 2 26 29 0.0 6 27 1 1 27 47 0.0 6 45 2 28 23 0.0 6 21 2 29 12 0.0 6 12 30 12 0.0 6 12 31 9 0.0 6 9 32 10 0.0 6 10 33 12 0.0 6 12 34 6 0.0 6 6 35 13 0.0 6 9 3 0 0 1 36 13 0.0 6 13 37 6 0.0 6 6 38 14 0.0 6 13 0 1 39 11 0.0 6 11 40 6 0.0 6 5 0 0 0 1 41 7 0.0 6 6 0 0 0 0 0 1 42 5 0.0 6 4 1 43 9 0.0 6 9 44 3 0.0 6 3 45 4 0.0 6 4 46 4 0.0 6 4 47 3 0.0 6 3 48 4 0.0 6 4 49 2 0.0 6 2 50 6 0.0 6 4 1 1 51 4 0.0 6 4 52 3 0.0 6 3 53 1 0.0 6 1 54 6 0.0 6 6 55 1 0.0 6 1 56 6 0.0 6 6 57 5 0.0 6 4 1 58 9 0.0 6 9 59 7 0.0 6 7 60 8 0.0 6 8 61 7 0.0 6 5 1 1 62 9 0.0 6 9 63 7 0.0 6 7 64 9 0.0 6 9 65 12 0.0 6 11 1 66 5 0.0 6 4 1 67 5 0.0 6 5 68 4 0.0 6 4 69 4 0.0 6 4 70 7 0.0 6 7 71 7 0.0 6 7 72 7 0.0 6 7 73 6 0.0 6 5 1 74 7 0.0 6 7 75 13 0.0 6 13 76 12 0.0 6 11 1 77 7 0.0 6 7 78 7 0.0 6 7 79 8 0.0 6 8 80 3 0.0 6 3 81 9 0.0 6 9 82 4 0.0 6 4 83 8 0.0 6 8 84 10 0.0 6 10 85 6 0.0 6 6 86 19 0.0 6 19 87 3 0.0 6 2 0 1 88 5 0.0 6 5 89 9 0.0 6 9 90 4 0.0 6 4 91 9 0.0 6 9 92 4 0.0 6 4 93 6 0.0 6 4 1 0 1 94 11 0.0 6 11 95 9 0.0 6 8 1 96 5 0.0 6 5 97 3 0.0 6 3 98 12 0.0 6 12 99 3 0.0 6 3 100 8 0.0 6 8 101 7 0.0 6 7 102 14 0.0 6 14 103 22 0.0 6 21 1 104 27 0.0 6 25 2 105 19 0.0 6 19 106 30 0.0 6 29 1 107 27 0.0 6 23 3 0 0 0 0 1 108 12 0.0 6 12 109 21 0.0 6 21 110 17 0.0 6 16 1 111 19 0.0 6 17 2 112 17 0.0 6 16 1 113 14 0.0 6 14 114 23 0.0 6 23 115 24 0.0 6 24 116 17 0.0 6 14 2 0 0 1 117 18 0.0 6 18 118 15 0.0 6 15 119 9 0.0 6 8 0 0 0 0 0 1 120 18 0.0 6 17 0 0 0 0 1 121 23 0.0 6 22 1 122 18 0.0 6 17 1 123 21 0.0 6 20 1 124 22 0.0 6 22 125 36 0.0 6 36 126 25 0.0 6 24 1 127 41 0.0 6 40 1 128 47 0.0 6 45 2 129 47 0.0 6 45 1 1 130 47 0.0 6 46 1 131 29 0.0 6 29 132 45 0.0 6 44 1 133 41 0.0 6 41 134 43 0.0 6 42 0 0 1 135 30 0.0 6 30 136 52 0.0 6 52 137 54 0.0 6 53 1 138 75 0.0 6 75 139 63 0.0 6 63 140 68 0.0 6 68 141 93 0.0 6 93 142 169 0.0 6 169 143 1133 0.0 6 1131 2 144 756 0.0 6 754 2 145 733 0.0 6 728 5 146 193 0.0 6 192 1 147 698 0.0 6 695 2 0 0 1 148 253 0.0 6 251 2 149 1809 0.0 6 1808 1 150 260 0.0 6 258 2 151 92 0.0 6 91 1 152 95 0.0 6 94 0 0 1 153 74 0.0 6 74 154 79 0.0 6 79 155 98 0.0 6 98 156 129 0.0 6 129 157 127 0.0 6 124 3 158 179 0.0 6 179 159 195 0.0 6 194 1 160 299 0.0 6 297 2 161 1756 0.0 6 1750 5 0 1 162 1736 0.0 6 1733 3 163 1786 0.0 6 1781 4 0 0 0 1 164 414 0.0 6 414 165 304 0.0 6 301 3 166 1988 0.0 6 1984 4 167 303 0.0 6 302 1 168 151 0.0 6 150 0 1 169 200 0.0 6 197 3 170 318 0.0 6 315 3 171 1996 0.0 6 1993 3 172 510 0.0 6 510 173 275 0.0 6 268 6 0 0 1 174 396 0.0 6 390 5 1 175 1956 0.0 6 1951 3 0 0 0 2 176 1672 0.0 6 1670 1 0 0 0 1 177 442 0.0 6 439 3 178 529 0.0 6 526 3 179 2109 0.0 6 2106 3 180 520 0.0 6 513 7 181 1620 0.0 6 1615 5 182 410 0.0 6 408 2 183 155 0.0 6 154 1 184 136 0.0 6 135 1 185 184 0.0 6 180 3 1 186 192 0.0 6 190 2 187 298 0.0 6 297 1 188 197 0.0 6 197 189 237 0.0 6 235 2 190 416 0.0 6 408 7 1 191 1864 0.0 6 1861 3 192 409 0.0 6 407 2 193 327 0.0 6 323 4 194 393 0.0 6 390 3 195 511 0.0 6 506 4 1 196 1149 0.0 6 1148 1 197 4605 0.0 6 4593 12 198 1425 0.0 6 1419 6 199 3638 0.0 6 3631 7 200 389 0.0 6 388 1 201 291 0.0 6 288 3 202 316 0.0 6 313 2 0 0 1 203 412 0.0 6 409 3 204 451 0.0 6 447 3 1 205 1208 0.0 6 1200 8 206 567 0.0 6 564 3 207 841 0.0 6 834 7 208 1049 0.0 6 1046 3 209 1384 0.0 6 1377 7 210 1392 0.0 6 1390 2 211 2948 0.0 6 2943 5 212 922 0.0 6 920 2 213 986 0.0 6 979 7 214 1484 0.0 6 1476 8 215 4010 0.0 6 4002 5 3 216 859 0.0 6 852 7 217 549 0.0 6 544 5 218 973 0.0 6 965 8 219 1018 0.0 6 1014 3 0 0 0 0 1 220 1893 0.0 6 1888 5 221 341 0.0 6 337 4 222 422 0.0 6 416 6 223 459 0.0 6 455 3 1 224 576 0.0 6 570 6 225 807 0.0 6 800 7 226 992 0.0 6 985 6 0 0 1 227 1226 0.0 6 1217 9 228 1000 0.0 6 995 4 1 229 1250 0.0 6 1243 7 230 2899 0.0 6 2890 8 0 0 1 231 344 0.0 6 325 19 232 264 0.0 6 261 3 233 216 0.0 6 210 6 234 281 0.0 6 274 7 235 250 0.0 6 246 4 236 150 0.0 6 146 4 237 232 0.0 6 230 2 238 166 0.0 6 165 1 239 195 0.0 6 184 10 1 240 311 0.0 6 308 2 1 241 389 0.0 6 379 10 242 311 0.0 6 301 9 0 1 243 364 0.0 6 352 12 244 225 0.0 6 215 10 245 319 0.0 6 306 11 2 246 189 0.0 6 184 5 247 208 0.0 6 203 5 248 205 0.0 6 202 2 0 1 249 363 0.0 6 360 3 250 270 0.0 6 266 4 251 256 0.0 6 250 5 1 252 331 0.0 6 322 9 253 300 0.0 6 290 10 254 279 0.0 6 266 13 255 205 0.0 6 202 2 1 256 155 0.0 6 150 5 257 194 0.0 6 191 3 258 237 0.0 6 228 8 1 259 332 0.0 6 317 14 0 0 1 260 579 0.0 6 568 10 0 0 0 1 261 210 0.0 6 206 4 262 259 0.0 6 244 15 263 621 0.0 6 605 15 1 264 373 0.0 6 365 7 0 0 0 1 265 348 0.0 6 343 5 266 322 0.0 6 316 5 1 267 309 0.0 6 297 11 0 0 0 1 268 285 0.0 6 279 5 0 0 0 0 1 269 350 0.0 6 342 8 270 198 0.0 6 193 5 271 407 0.0 6 395 12 272 757 0.0 6 743 14 273 272 0.0 6 265 7 274 255 0.0 6 245 9 1 275 499 0.0 6 482 16 1 276 731 0.0 6 712 17 1 0 1 277 671 0.0 6 654 15 1 0 1 278 519 0.0 6 511 8 279 798 0.0 6 775 18 4 1 280 7478 0.0 6 7217 260 1 281 72 0.0 6 59 13 282 5 0.0 6 5 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 3278 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 1 0.0 6 0 1 68 1 0.0 6 1 70 14 0.0 6 14 71 57 0.0 6 57 72 28 0.0 6 28 73 22 0.0 6 22 74 7 0.0 6 7 75 30 0.0 6 30 76 23 0.0 6 23 77 77 0.0 6 77 78 3 0.0 6 3 80 2 0.0 6 2 84 1 0.0 6 1 88 27 0.0 6 27 89 73 0.0 6 73 90 83 0.0 6 83 91 58 0.0 6 58 92 1 0.0 6 1 93 30 0.0 6 30 94 77 0.0 6 77 95 6 0.0 6 6 96 1 0.0 6 1 97 3 0.0 6 3 98 31 0.0 6 31 99 88 0.0 6 88 100 5 0.0 6 5 101 1 0.0 6 1 102 31 0.0 6 31 103 90 0.0 6 90 104 53 0.0 6 53 105 3 0.0 6 3 106 30 0.0 6 29 1 107 95 0.0 6 95 108 30 0.0 6 30 109 58 0.0 6 58 110 3 0.0 6 3 116 1 0.0 6 1 117 1 0.0 6 1 118 20 0.0 6 20 119 77 0.0 6 77 120 6 0.0 6 6 122 1 0.0 6 1 123 4 0.0 6 4 124 43 0.0 6 43 125 159 0.0 6 159 126 53 0.0 6 53 127 142 0.0 6 142 128 11 0.0 6 11 131 2 0.0 6 2 132 18 0.0 6 18 133 36 0.0 6 36 134 5 0.0 6 5 136 2 0.0 6 2 137 3 0.0 6 3 138 66 0.0 6 66 139 166 0.0 6 166 140 7 0.0 6 7 141 5 0.0 6 5 142 88 0.0 6 88 143 243 0.0 6 243 144 12 0.0 6 12 146 4 0.0 6 4 147 25 0.0 6 25 148 60 0.0 6 60 149 3 0.0 6 3 153 1 0.0 6 1 154 1 0.0 6 1 155 5 0.0 6 5 156 11 0.0 6 11 157 125 0.0 6 125 158 349 0.0 6 349 159 16 0.0 6 15 1 160 1 0.0 6 1 179 4 0.0 6 4 180 61 0.0 6 61 181 121 0.0 6 121 182 6 0.0 6 6 183 3 0.0 6 3 203 1 0.0 6 1 209 1 0.0 6 1 224 1 0.0 6 1 229 2 0.0 6 2 232 24 0.0 6 24 233 81 0.0 6 80 1 234 6 0.0 6 6 268 1 0.0 6 1 271 6 0.0 6 6 272 6 0.0 6 6 275 1 0.0 6 1 294 12 0.0 6 12 295 14 0.0 6 14 306 4 0.0 6 4 307 8 0.0 6 8 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 104876 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1694.3 0 1 4 2 423.6 1 1 1 6 2 26.5 1 1 1 9 1 0.4 2 1 10 5 0.1 3 4 1 11 3 0.0 3 3 12 2 0.0 3 1 1 13 12 0.0 3 11 1 14 7 0.0 4 5 2 16 16 0.0 4 14 2 17 22 0.0 5 19 3 18 13 0.0 5 10 2 1 19 11 0.0 5 11 20 120 0.0 6 114 6 21 123 0.0 6 119 4 22 48 0.0 6 43 5 23 31 0.0 6 31 24 38 0.0 6 36 0 2 25 30 0.0 6 29 0 1 26 27 0.0 6 26 0 0 0 1 27 18 0.0 6 16 1 1 28 17 0.0 6 16 1 29 22 0.0 6 21 1 30 20 0.0 6 20 31 7 0.0 6 7 32 13 0.0 6 10 2 1 33 19 0.0 6 16 2 0 0 0 1 34 10 0.0 6 10 35 14 0.0 6 12 2 36 8 0.0 6 7 0 0 0 0 0 1 37 10 0.0 6 10 38 13 0.0 6 12 0 0 0 0 1 39 10 0.0 6 9 1 40 13 0.0 6 12 1 41 16 0.0 6 15 1 42 11 0.0 6 7 4 43 12 0.0 6 8 4 44 19 0.0 6 17 2 45 17 0.0 6 16 1 46 20 0.0 6 19 1 47 24 0.0 6 23 1 48 24 0.0 6 20 3 0 0 0 1 49 16 0.0 6 13 3 50 12 0.0 6 8 3 0 0 1 51 16 0.0 6 13 3 52 33 0.0 6 30 3 53 18 0.0 6 15 1 0 1 0 0 1 54 19 0.0 6 17 0 1 0 1 55 19 0.0 6 17 1 1 56 16 0.0 6 15 1 57 23 0.0 6 23 58 22 0.0 6 18 3 1 59 24 0.0 6 22 1 1 60 21 0.0 6 19 2 61 13 0.0 6 13 62 25 0.0 6 22 3 63 31 0.0 6 30 1 64 32 0.0 6 32 65 19 0.0 6 18 0 0 0 0 0 1 66 35 0.0 6 33 1 0 0 0 0 1 67 193 0.0 6 186 5 0 0 1 0 1 68 52 0.0 6 45 4 1 0 0 2 69 100 0.0 6 93 7 70 30 0.0 6 28 2 71 49 0.0 6 45 4 72 28 0.0 6 28 73 21 0.0 6 19 2 74 29 0.0 6 28 0 0 0 1 75 19 0.0 6 17 2 76 28 0.0 6 27 1 77 30 0.0 6 27 2 1 78 37 0.0 6 36 1 79 36 0.0 6 35 0 1 80 27 0.0 6 25 1 1 81 27 0.0 6 25 1 0 0 0 1 82 44 0.0 6 44 83 70 0.0 6 68 2 84 33 0.0 6 28 3 0 0 0 1 1 85 23 0.0 6 21 2 86 23 0.0 6 23 87 34 0.0 6 33 0 1 88 21 0.0 6 19 2 89 42 0.0 6 39 1 1 0 0 0 1 90 50 0.0 6 49 0 0 0 0 0 1 91 61 0.0 6 58 3 92 41 0.0 6 39 2 93 69 0.0 6 68 1 94 48 0.0 6 45 2 0 0 1 95 64 0.0 6 59 4 1 96 54 0.0 6 51 3 97 66 0.0 6 62 4 98 59 0.0 6 55 2 2 99 49 0.0 6 48 0 0 0 0 1 100 54 0.0 6 52 1 0 0 0 1 101 62 0.0 6 58 2 1 0 1 102 73 0.0 6 72 1 103 100 0.0 6 97 3 104 104 0.0 6 97 4 2 0 0 0 1 105 78 0.0 6 76 1 1 106 66 0.0 6 60 4 1 1 107 81 0.0 6 77 4 108 83 0.0 6 81 0 2 109 82 0.0 6 80 2 110 72 0.0 6 69 3 111 102 0.0 6 101 1 112 106 0.0 6 102 1 1 0 0 1 1 113 108 0.0 6 99 8 1 114 119 0.0 6 117 2 115 107 0.0 6 106 1 116 105 0.0 6 102 3 117 109 0.0 6 108 0 0 0 1 118 128 0.0 6 125 2 0 0 1 119 101 0.0 6 97 4 120 137 0.0 6 133 3 1 121 109 0.0 6 106 3 122 149 0.0 6 146 3 123 124 0.0 6 119 5 124 176 0.0 6 174 2 125 204 0.0 6 199 4 0 0 0 0 1 126 175 0.0 6 171 4 127 201 0.0 6 199 2 128 222 0.0 6 218 4 129 203 0.0 6 197 5 0 0 0 1 130 218 0.0 6 215 3 131 238 0.0 6 233 4 1 132 296 0.0 6 292 4 133 377 0.0 6 371 4 1 0 0 1 134 399 0.0 6 390 8 0 0 1 135 481 0.0 6 474 6 1 136 415 0.0 6 413 2 137 471 0.0 6 466 4 1 138 514 0.0 6 509 4 1 139 1257 0.0 6 1252 4 0 0 1 140 985 0.0 6 978 7 141 1079 0.0 6 1067 11 0 0 1 142 906 0.0 6 895 8 0 0 2 1 143 1035 0.0 6 1027 6 0 0 2 144 959 0.0 6 947 9 0 0 2 0 1 145 1944 0.0 6 1932 10 0 0 2 146 778 0.0 6 771 6 0 0 1 147 526 0.0 6 518 8 148 671 0.0 6 656 13 0 0 1 1 149 825 0.0 6 806 15 1 0 3 150 1414 0.0 6 1397 13 2 0 0 0 2 151 826 0.0 6 817 7 0 1 0 1 152 1350 0.0 6 1338 11 0 0 0 1 153 1330 0.0 6 1321 9 154 1151 0.0 6 1139 9 0 0 2 0 1 155 1547 0.0 6 1536 10 0 1 156 1493 0.0 6 1477 14 0 1 1 157 2523 0.0 6 2508 10 1 0 1 2 1 158 2012 0.0 6 2000 11 0 0 0 0 1 159 1438 0.0 6 1428 6 1 0 1 0 2 160 1200 0.0 6 1191 7 0 0 1 0 1 161 1037 0.0 6 1027 6 2 1 0 1 162 1342 0.0 6 1335 6 0 0 1 163 965 0.0 6 958 7 164 921 0.0 6 909 10 0 0 1 1 165 931 0.0 6 914 13 1 0 1 1 1 166 1304 0.0 6 1286 17 1 167 1857 0.0 6 1836 19 1 0 0 1 168 1384 0.0 6 1365 19 169 1302 0.0 6 1290 9 2 0 0 0 1 170 1442 0.0 6 1430 9 1 0 0 0 2 171 2257 0.0 6 2246 10 0 0 0 0 1 172 2187 0.0 6 2181 3 0 0 0 1 2 173 1517 0.0 6 1503 9 2 0 1 0 2 174 1520 0.0 6 1507 11 0 0 0 2 175 2236 0.0 6 2212 19 0 1 0 3 1 176 1582 0.0 6 1568 11 0 0 0 2 1 177 1678 0.0 6 1664 12 1 0 1 178 1154 0.0 6 1139 14 0 0 1 179 851 0.0 6 839 10 0 0 0 1 1 180 985 0.0 6 973 10 1 0 1 181 1255 0.0 6 1243 10 1 0 1 182 808 0.0 6 800 7 0 0 0 1 183 996 0.0 6 983 11 2 184 2032 0.0 6 2018 9 0 0 0 2 3 185 1609 0.0 6 1596 12 0 0 0 0 1 186 1865 0.0 6 1834 28 0 0 1 1 1 187 2144 0.0 6 2121 21 0 0 1 0 1 188 1531 0.0 6 1511 17 0 0 1 1 1 189 1450 0.0 6 1424 23 0 0 2 0 1 190 1764 0.0 6 1731 28 1 0 1 3 191 1544 0.0 6 1522 18 1 0 0 0 3 192 1353 0.0 6 1333 15 0 1 1 2 1 193 1233 0.0 6 1216 17 194 1454 0.0 6 1425 26 2 0 1 195 1357 0.0 6 1329 24 1 1 1 1 196 1699 0.0 6 1665 34 197 804 0.0 6 782 19 1 1 1 198 988 0.0 6 954 31 1 0 1 1 199 1523 0.0 6 1489 32 0 0 0 1 1 200 763 0.0 6 747 14 1 0 0 1 201 705 0.0 6 679 22 1 0 1 1 1 202 648 0.0 6 640 7 0 0 0 1 203 587 0.0 6 582 5 204 687 0.0 6 674 13 205 443 0.0 6 438 5 206 451 0.0 6 450 1 207 517 0.0 6 513 4 208 417 0.0 6 411 5 0 0 1 209 646 0.0 6 639 7 210 311 0.0 6 305 6 211 502 0.0 6 500 2 212 196 0.0 6 188 7 1 213 157 0.0 6 156 1 214 302 0.0 6 295 7 215 275 0.0 6 267 8 216 365 0.0 6 357 7 0 0 0 1 217 122 0.0 6 120 2 218 130 0.0 6 124 5 1 219 148 0.0 6 144 4 220 57 0.0 6 57 221 160 0.0 6 156 4 222 339 0.0 6 330 7 0 0 0 2 223 417 0.0 6 405 10 1 1 224 176 0.0 6 173 2 0 0 0 1 225 415 0.0 6 410 5 226 344 0.0 6 340 4 227 167 0.0 6 159 8 228 118 0.0 6 115 3 229 138 0.0 6 132 6 230 123 0.0 6 118 4 0 0 1 231 157 0.0 6 154 3 232 154 0.0 6 149 5 233 83 0.0 6 80 3 234 53 0.0 6 52 1 235 37 0.0 6 37 236 15 0.0 6 15 237 16 0.0 6 16 238 89 0.0 6 88 1 239 65 0.0 6 63 2 240 33 0.0 6 31 2 241 90 0.0 6 87 3 242 54 0.0 6 51 3 243 59 0.0 6 56 3 244 38 0.0 6 38 245 33 0.0 6 32 1 246 23 0.0 6 23 247 14 0.0 6 13 1 248 57 0.0 6 55 2 249 7 0.0 6 6 1 250 5 0.0 6 5 251 15 0.0 6 15 252 1 0.0 6 1 253 22 0.0 6 21 1 254 9 0.0 6 9 255 1 0.0 6 1 260 1 0.0 6 1 275 1 0.0 6 0 1 277 3 0.0 6 1 2 278 22 0.0 6 22 279 30 0.0 6 29 1 280 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 3498 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 48 1 0.0 6 0 0 1 49 1 0.0 6 0 1 53 1 0.0 6 1 54 1 0.0 6 1 55 2 0.0 6 2 59 1 0.0 6 0 0 0 0 1 62 1 0.0 6 1 64 1 0.0 6 1 69 1 0.0 6 1 70 2 0.0 6 2 71 6 0.0 6 6 72 50 0.0 6 50 73 73 0.0 6 70 3 74 55 0.0 6 53 2 75 6 0.0 6 6 76 27 0.0 6 27 77 21 0.0 6 21 78 92 0.0 6 92 79 1 0.0 6 1 83 1 0.0 6 0 1 85 1 0.0 6 1 87 2 0.0 6 2 88 5 0.0 6 5 89 19 0.0 6 19 90 114 0.0 6 113 1 91 104 0.0 6 104 92 82 0.0 6 82 93 5 0.0 6 5 94 18 0.0 6 16 0 0 1 1 95 102 0.0 6 99 1 0 0 1 0 1 96 1 0.0 6 1 98 6 0.0 6 5 1 99 23 0.0 6 23 100 100 0.0 6 100 101 5 0.0 6 5 102 5 0.0 6 4 1 103 21 0.0 6 21 104 109 0.0 6 109 105 74 0.0 6 74 106 5 0.0 6 4 0 0 1 107 17 0.0 6 17 108 122 0.0 6 122 109 18 0.0 6 18 110 68 0.0 6 68 111 1 0.0 6 1 112 2 0.0 6 0 0 0 1 1 113 1 0.0 6 0 0 0 1 116 1 0.0 6 1 117 1 0.0 6 1 118 2 0.0 6 2 119 14 0.0 6 14 120 81 0.0 6 81 121 1 0.0 6 1 124 6 0.0 6 6 125 46 0.0 6 45 1 126 178 0.0 6 177 1 127 34 0.0 6 34 128 200 0.0 6 200 129 1 0.0 6 1 130 1 0.0 6 0 0 0 0 1 131 1 0.0 6 1 132 2 0.0 6 2 133 10 0.0 6 10 134 61 0.0 6 61 135 2 0.0 6 2 137 2 0.0 6 2 138 3 0.0 6 1 0 0 0 2 139 47 0.0 6 47 140 260 0.0 6 260 141 9 0.0 6 9 142 5 0.0 6 5 143 53 0.0 6 53 144 282 0.0 6 279 1 0 0 2 145 9 0.0 6 8 0 0 0 1 147 12 0.0 6 12 148 23 0.0 6 21 0 0 0 2 149 67 0.0 6 67 150 1 0.0 6 1 152 1 0.0 6 0 0 0 1 153 1 0.0 6 1 155 1 0.0 6 1 156 3 0.0 6 2 1 157 10 0.0 6 9 1 158 93 0.0 6 93 159 388 0.0 6 388 160 11 0.0 6 11 161 1 0.0 6 0 0 0 1 162 2 0.0 6 2 163 1 0.0 6 0 0 0 0 1 177 1 0.0 6 1 178 2 0.0 6 2 179 2 0.0 6 2 180 2 0.0 6 2 181 39 0.0 6 39 182 129 0.0 6 129 183 4 0.0 6 4 184 2 0.0 6 2 185 1 0.0 6 1 188 1 0.0 6 1 202 1 0.0 6 1 210 1 0.0 6 1 229 2 0.0 6 2 233 3 0.0 6 3 234 6 0.0 6 6 235 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_16_103_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_16_330_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_16_103_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_16_330_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.24 s (79 µs/read; 0.76 M reads/minute). === Summary === Total read pairs processed: 66,259 Read 1 with adapter: 66,254 (100.0%) Read 2 with adapter: 66,225 (99.9%) == Read fate breakdown == Pairs that were too short: 4 (0.0%) Pairs discarded as untrimmed: 39 (0.1%) Pairs written (passing filters): 66,216 (99.9%) Total basepairs processed: 32,565,382 bp Read 1: 18,491,569 bp Read 2: 14,073,813 bp Total written (filtered): 4,071,699 bp (12.5%) Read 1: 2,070,456 bp Read 2: 2,001,243 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 65943 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.8% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 2 258.8 1 0 2 6 1 16.2 1 0 0 1 7 2 4.0 2 0 0 2 8 1 1.0 2 1 10 1 0.1 3 1 13 1 0.0 3 0 1 14 1 0.0 4 1 16 1 0.0 4 1 17 3 0.0 5 2 0 1 19 2 0.0 5 2 20 37 0.0 6 37 21 10 0.0 6 10 22 3 0.0 6 3 23 8 0.0 6 8 24 2 0.0 6 2 25 9 0.0 6 9 26 9 0.0 6 8 1 27 5 0.0 6 5 28 3 0.0 6 3 29 2 0.0 6 2 30 2 0.0 6 1 1 31 2 0.0 6 2 32 2 0.0 6 2 33 2 0.0 6 2 34 5 0.0 6 5 35 4 0.0 6 4 36 2 0.0 6 2 37 1 0.0 6 1 38 4 0.0 6 4 40 3 0.0 6 3 41 1 0.0 6 1 42 1 0.0 6 1 43 1 0.0 6 1 44 6 0.0 6 5 1 45 2 0.0 6 2 46 6 0.0 6 6 47 2 0.0 6 2 48 1 0.0 6 1 49 4 0.0 6 3 1 50 4 0.0 6 3 1 51 1 0.0 6 1 52 2 0.0 6 2 53 4 0.0 6 4 54 4 0.0 6 4 55 3 0.0 6 3 56 3 0.0 6 3 57 4 0.0 6 4 58 1 0.0 6 1 60 2 0.0 6 2 61 4 0.0 6 4 62 3 0.0 6 3 63 2 0.0 6 1 1 64 1 0.0 6 1 65 2 0.0 6 2 67 1 0.0 6 1 68 2 0.0 6 2 69 1 0.0 6 0 0 1 70 2 0.0 6 2 72 2 0.0 6 1 1 73 2 0.0 6 2 74 1 0.0 6 1 76 2 0.0 6 2 77 3 0.0 6 3 78 3 0.0 6 3 79 1 0.0 6 1 80 2 0.0 6 2 81 3 0.0 6 3 82 1 0.0 6 1 83 3 0.0 6 3 84 2 0.0 6 1 1 86 13 0.0 6 13 89 3 0.0 6 3 90 2 0.0 6 2 91 1 0.0 6 1 92 4 0.0 6 4 93 1 0.0 6 1 94 3 0.0 6 3 95 2 0.0 6 2 96 2 0.0 6 1 1 97 7 0.0 6 7 98 7 0.0 6 7 99 2 0.0 6 2 100 5 0.0 6 5 101 12 0.0 6 11 1 102 9 0.0 6 9 103 3 0.0 6 3 104 4 0.0 6 4 105 3 0.0 6 2 0 0 0 0 0 1 106 3 0.0 6 1 2 107 2 0.0 6 1 1 108 9 0.0 6 8 1 109 2 0.0 6 2 110 1 0.0 6 1 111 5 0.0 6 5 112 9 0.0 6 8 1 113 4 0.0 6 3 1 114 6 0.0 6 6 115 7 0.0 6 7 116 3 0.0 6 3 117 8 0.0 6 8 118 5 0.0 6 4 1 119 6 0.0 6 6 120 5 0.0 6 5 121 8 0.0 6 7 1 122 12 0.0 6 11 1 123 6 0.0 6 6 124 6 0.0 6 5 1 125 11 0.0 6 11 126 8 0.0 6 8 127 13 0.0 6 11 2 128 17 0.0 6 17 129 21 0.0 6 19 2 130 10 0.0 6 10 131 36 0.0 6 35 1 132 20 0.0 6 19 1 133 15 0.0 6 15 134 18 0.0 6 18 135 19 0.0 6 16 2 0 0 0 1 136 17 0.0 6 14 3 137 25 0.0 6 25 138 31 0.0 6 29 1 1 139 19 0.0 6 17 2 140 44 0.0 6 44 141 254 0.0 6 253 0 0 1 142 52 0.0 6 52 143 31 0.0 6 30 1 144 252 0.0 6 251 0 0 1 145 180 0.0 6 178 2 146 130 0.0 6 130 147 238 0.0 6 237 0 0 0 0 0 1 148 53 0.0 6 53 149 29 0.0 6 29 150 36 0.0 6 32 4 151 41 0.0 6 41 152 19 0.0 6 19 153 19 0.0 6 19 154 23 0.0 6 22 1 155 18 0.0 6 17 0 1 156 24 0.0 6 23 1 157 26 0.0 6 26 158 31 0.0 6 31 159 38 0.0 6 37 1 160 57 0.0 6 57 161 447 0.0 6 445 0 1 0 0 0 1 162 80 0.0 6 80 163 51 0.0 6 48 3 164 76 0.0 6 73 3 165 333 0.0 6 332 1 166 254 0.0 6 254 167 65 0.0 6 64 1 168 75 0.0 6 75 169 368 0.0 6 364 4 170 93 0.0 6 93 171 68 0.0 6 67 1 172 293 0.0 6 292 1 173 167 0.0 6 166 1 174 846 0.0 6 844 2 175 174 0.0 6 174 176 65 0.0 6 65 177 88 0.0 6 84 4 178 53 0.0 6 52 1 179 310 0.0 6 308 2 180 157 0.0 6 157 181 78 0.0 6 76 1 0 1 182 75 0.0 6 74 0 0 1 183 372 0.0 6 368 3 0 0 0 0 1 184 108 0.0 6 106 2 185 134 0.0 6 130 4 186 79 0.0 6 77 1 1 187 60 0.0 6 59 1 188 141 0.0 6 138 3 189 131 0.0 6 127 4 190 120 0.0 6 116 4 191 175 0.0 6 170 5 192 199 0.0 6 195 4 193 147 0.0 6 142 5 194 100 0.0 6 97 3 195 178 0.0 6 172 6 196 202 0.0 6 196 6 197 199 0.0 6 194 5 198 257 0.0 6 249 7 0 0 0 1 199 210 0.0 6 200 10 200 271 0.0 6 260 10 1 201 238 0.0 6 233 4 1 202 301 0.0 6 296 5 203 486 0.0 6 476 9 0 0 0 0 1 204 379 0.0 6 373 6 205 279 0.0 6 271 6 2 206 310 0.0 6 301 9 207 506 0.0 6 500 6 208 973 0.0 6 964 9 209 328 0.0 6 319 9 210 206 0.0 6 200 6 211 154 0.0 6 152 2 212 145 0.0 6 140 4 0 1 213 289 0.0 6 285 4 214 439 0.0 6 437 2 215 283 0.0 6 278 5 216 615 0.0 6 609 6 217 333 0.0 6 324 9 218 292 0.0 6 289 3 219 233 0.0 6 226 7 220 183 0.0 6 179 4 221 188 0.0 6 180 8 222 292 0.0 6 285 6 0 1 223 274 0.0 6 269 4 1 224 186 0.0 6 182 4 225 355 0.0 6 349 6 226 421 0.0 6 406 13 2 227 321 0.0 6 318 2 0 1 228 294 0.0 6 286 8 229 272 0.0 6 266 5 0 1 230 234 0.0 6 231 3 231 199 0.0 6 191 8 232 242 0.0 6 233 9 233 281 0.0 6 278 3 234 259 0.0 6 252 7 235 202 0.0 6 196 6 236 172 0.0 6 165 5 2 237 253 0.0 6 245 7 0 1 238 256 0.0 6 253 3 239 275 0.0 6 271 4 240 321 0.0 6 308 12 1 241 505 0.0 6 493 11 1 242 370 0.0 6 360 10 243 264 0.0 6 250 14 244 286 0.0 6 279 7 245 448 0.0 6 437 11 246 209 0.0 6 204 5 247 274 0.0 6 268 6 248 208 0.0 6 202 5 1 249 350 0.0 6 345 5 250 331 0.0 6 326 5 251 315 0.0 6 310 4 1 252 302 0.0 6 299 3 253 190 0.0 6 180 8 2 254 230 0.0 6 227 3 255 218 0.0 6 209 9 256 292 0.0 6 285 7 257 228 0.0 6 226 2 258 243 0.0 6 233 8 1 1 259 390 0.0 6 380 10 260 482 0.0 6 474 6 2 261 222 0.0 6 215 6 0 1 262 254 0.0 6 245 9 263 517 0.0 6 509 7 1 264 267 0.0 6 263 4 265 275 0.0 6 271 4 266 227 0.0 6 222 5 267 254 0.0 6 244 10 268 379 0.0 6 363 15 0 0 1 269 469 0.0 6 459 10 270 331 0.0 6 326 5 271 336 0.0 6 327 8 0 1 272 448 0.0 6 435 12 1 273 501 0.0 6 487 14 274 580 0.0 6 567 12 1 275 303 0.0 6 290 12 1 276 944 0.0 6 911 32 0 0 1 277 1611 0.0 6 1568 41 2 278 2138 0.0 6 2079 57 2 279 6316 0.0 6 6154 157 4 0 0 0 1 280 21409 0.0 6 20795 600 8 6 281 154 0.0 6 128 23 0 1 2 282 8 0.0 6 8 283 5 0.0 6 4 1 284 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 311 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 1 0.0 6 1 70 3 0.0 6 3 71 6 0.0 6 6 72 1 0.0 6 1 73 1 0.0 6 1 74 10 0.0 6 10 75 6 0.0 6 6 76 8 0.0 6 8 77 2 0.0 6 2 78 1 0.0 6 1 79 2 0.0 6 2 90 6 0.0 6 6 91 9 0.0 6 9 92 2 0.0 6 2 93 1 0.0 6 0 1 94 6 0.0 6 6 95 13 0.0 6 13 96 6 0.0 6 6 98 7 0.0 6 7 99 6 0.0 6 6 101 4 0.0 6 4 102 3 0.0 6 3 103 4 0.0 6 4 104 28 0.0 6 28 105 4 0.0 6 4 107 1 0.0 6 1 108 2 0.0 6 2 109 8 0.0 6 8 110 6 0.0 6 6 112 2 0.0 6 2 113 8 0.0 6 8 115 1 0.0 6 1 132 3 0.0 6 3 133 4 0.0 6 4 136 1 0.0 6 1 137 11 0.0 6 11 138 26 0.0 6 26 139 1 0.0 6 1 142 1 0.0 6 1 143 5 0.0 6 5 144 5 0.0 6 5 145 8 0.0 6 8 146 15 0.0 6 15 147 1 0.0 6 1 154 9 0.0 6 9 155 11 0.0 6 11 180 3 0.0 6 3 181 1 0.0 6 1 183 1 0.0 6 1 218 3 0.0 6 3 219 9 0.0 6 9 222 1 0.0 6 1 243 5 0.0 6 5 244 9 0.0 6 9 250 2 0.0 6 2 251 1 0.0 6 1 252 5 0.0 6 5 253 1 0.0 6 1 293 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 65907 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 258.8 1 0 1 6 2 16.2 1 2 9 1 0.3 2 1 11 1 0.0 3 1 12 2 0.0 3 2 13 16 0.0 3 13 3 16 14 0.0 4 13 0 1 17 16 0.0 5 14 2 18 6 0.0 5 5 1 19 7 0.0 5 6 1 20 46 0.0 6 46 21 47 0.0 6 46 1 22 41 0.0 6 40 1 23 23 0.0 6 21 1 1 24 16 0.0 6 16 25 12 0.0 6 10 2 26 13 0.0 6 11 2 27 6 0.0 6 6 28 5 0.0 6 5 29 16 0.0 6 15 1 30 14 0.0 6 12 1 1 31 9 0.0 6 8 1 32 3 0.0 6 3 33 5 0.0 6 5 34 13 0.0 6 13 35 4 0.0 6 3 1 36 7 0.0 6 6 1 37 5 0.0 6 5 38 10 0.0 6 6 4 39 13 0.0 6 13 40 8 0.0 6 8 41 7 0.0 6 6 1 42 3 0.0 6 3 43 4 0.0 6 2 2 44 13 0.0 6 11 2 45 7 0.0 6 7 46 19 0.0 6 17 1 1 47 22 0.0 6 20 2 48 7 0.0 6 5 2 49 23 0.0 6 19 3 1 50 22 0.0 6 18 4 51 4 0.0 6 4 52 18 0.0 6 15 2 1 53 13 0.0 6 11 2 54 23 0.0 6 22 1 55 11 0.0 6 9 1 1 56 15 0.0 6 14 0 1 57 13 0.0 6 11 2 58 9 0.0 6 8 1 59 7 0.0 6 6 1 60 9 0.0 6 9 61 7 0.0 6 6 1 62 10 0.0 6 9 1 63 10 0.0 6 10 64 16 0.0 6 15 1 65 7 0.0 6 7 66 16 0.0 6 11 5 67 11 0.0 6 11 68 29 0.0 6 28 0 1 69 103 0.0 6 100 2 1 70 25 0.0 6 22 3 71 69 0.0 6 64 5 72 33 0.0 6 31 2 73 21 0.0 6 20 1 74 16 0.0 6 15 1 75 12 0.0 6 11 0 1 76 28 0.0 6 23 4 1 77 8 0.0 6 7 1 78 21 0.0 6 21 79 25 0.0 6 23 2 80 20 0.0 6 19 1 81 21 0.0 6 21 82 16 0.0 6 15 0 1 83 29 0.0 6 28 1 84 26 0.0 6 24 2 85 37 0.0 6 34 3 86 15 0.0 6 15 87 26 0.0 6 24 2 88 13 0.0 6 12 1 89 32 0.0 6 30 2 90 33 0.0 6 32 1 91 36 0.0 6 34 2 92 13 0.0 6 12 1 93 51 0.0 6 46 4 1 94 81 0.0 6 76 4 1 95 42 0.0 6 39 0 2 0 1 96 34 0.0 6 31 3 97 40 0.0 6 38 2 98 38 0.0 6 31 6 1 99 30 0.0 6 28 2 100 48 0.0 6 44 4 101 40 0.0 6 39 1 102 61 0.0 6 58 3 103 63 0.0 6 58 4 0 0 1 104 64 0.0 6 60 4 105 57 0.0 6 53 4 106 46 0.0 6 46 107 54 0.0 6 50 4 108 37 0.0 6 34 2 0 0 1 109 54 0.0 6 48 6 110 42 0.0 6 41 1 111 47 0.0 6 43 3 1 112 37 0.0 6 36 1 113 33 0.0 6 32 1 114 57 0.0 6 56 1 115 50 0.0 6 48 2 116 33 0.0 6 32 0 1 117 49 0.0 6 47 1 1 118 59 0.0 6 57 1 1 119 57 0.0 6 53 3 0 0 1 120 119 0.0 6 110 9 121 74 0.0 6 70 3 0 1 122 73 0.0 6 69 3 1 123 78 0.0 6 72 6 124 79 0.0 6 74 5 125 64 0.0 6 61 2 1 126 68 0.0 6 65 3 127 93 0.0 6 84 9 128 62 0.0 6 56 5 1 129 55 0.0 6 52 2 1 130 62 0.0 6 60 2 131 100 0.0 6 96 4 132 128 0.0 6 125 2 1 133 89 0.0 6 87 1 0 0 1 134 111 0.0 6 110 1 135 292 0.0 6 280 11 1 136 193 0.0 6 188 4 1 137 238 0.0 6 233 4 1 138 178 0.0 6 174 4 139 322 0.0 6 315 7 140 257 0.0 6 254 3 141 244 0.0 6 238 6 142 493 0.0 6 481 10 1 0 0 0 1 143 390 0.0 6 379 9 0 1 1 144 459 0.0 6 454 5 145 336 0.0 6 327 8 1 146 239 0.0 6 236 2 1 147 164 0.0 6 156 7 1 148 242 0.0 6 235 7 149 278 0.0 6 268 8 1 0 0 1 150 457 0.0 6 450 7 151 219 0.0 6 212 5 1 1 152 697 0.0 6 681 16 153 323 0.0 6 314 9 154 434 0.0 6 420 12 1 0 1 155 543 0.0 6 522 21 156 402 0.0 6 391 10 1 157 575 0.0 6 563 11 1 158 634 0.0 6 619 15 159 610 0.0 6 592 16 1 1 160 631 0.0 6 618 13 161 380 0.0 6 372 7 1 162 387 0.0 6 378 9 163 567 0.0 6 553 10 4 164 429 0.0 6 422 4 3 165 515 0.0 6 499 15 1 166 588 0.0 6 573 14 0 0 1 167 441 0.0 6 429 12 168 679 0.0 6 657 19 1 0 2 169 295 0.0 6 288 6 0 1 170 567 0.0 6 550 16 1 171 661 0.0 6 642 19 172 555 0.0 6 548 6 0 0 1 173 392 0.0 6 381 10 0 1 174 599 0.0 6 580 18 1 175 1019 0.0 6 984 34 0 0 1 176 681 0.0 6 655 26 177 881 0.0 6 858 21 0 0 1 1 178 737 0.0 6 720 14 1 1 1 179 799 0.0 6 779 19 1 180 789 0.0 6 764 23 0 1 1 181 817 0.0 6 792 23 2 182 341 0.0 6 331 8 1 0 1 183 648 0.0 6 631 15 1 0 1 184 281 0.0 6 270 8 1 1 1 185 357 0.0 6 342 14 1 186 1468 0.0 6 1426 41 0 0 1 187 1256 0.0 6 1214 39 1 0 0 1 1 188 1219 0.0 6 1177 38 3 1 189 1124 0.0 6 1084 35 0 3 1 0 1 190 1800 0.0 6 1748 49 1 0 2 191 1330 0.0 6 1292 37 0 0 0 1 192 878 0.0 6 855 23 193 762 0.0 6 736 25 1 194 1202 0.0 6 1158 40 3 0 0 1 195 1343 0.0 6 1304 39 196 1924 0.0 6 1874 44 3 1 2 197 668 0.0 6 642 22 2 1 1 198 2166 0.0 6 2093 67 4 1 1 199 2745 0.0 6 2671 72 1 0 0 1 200 1147 0.0 6 1111 35 1 201 1259 0.0 6 1218 39 1 0 1 202 716 0.0 6 684 31 1 203 848 0.0 6 824 23 0 0 1 204 1256 0.0 6 1231 24 0 0 1 205 407 0.0 6 399 8 206 446 0.0 6 434 12 207 439 0.0 6 428 11 208 397 0.0 6 385 12 209 316 0.0 6 306 10 210 289 0.0 6 285 4 211 347 0.0 6 337 8 1 1 212 246 0.0 6 236 9 0 0 1 213 123 0.0 6 119 3 1 214 277 0.0 6 271 6 215 353 0.0 6 337 16 216 836 0.0 6 810 26 217 611 0.0 6 589 22 218 512 0.0 6 495 16 1 219 478 0.0 6 460 18 220 441 0.0 6 426 15 221 435 0.0 6 426 9 222 209 0.0 6 200 9 223 491 0.0 6 477 13 1 224 429 0.0 6 417 12 225 533 0.0 6 509 22 0 0 2 226 478 0.0 6 461 16 1 227 270 0.0 6 257 11 0 1 0 1 228 235 0.0 6 229 6 229 306 0.0 6 297 9 230 230 0.0 6 224 4 1 1 231 228 0.0 6 218 10 232 297 0.0 6 288 9 233 369 0.0 6 364 5 234 192 0.0 6 187 5 235 77 0.0 6 72 5 236 57 0.0 6 54 3 237 53 0.0 6 52 0 0 1 238 27 0.0 6 26 1 239 27 0.0 6 25 2 240 121 0.0 6 118 3 241 103 0.0 6 100 3 242 161 0.0 6 156 4 1 243 261 0.0 6 255 6 244 103 0.0 6 101 2 245 49 0.0 6 46 3 246 50 0.0 6 50 247 44 0.0 6 42 2 248 34 0.0 6 34 249 26 0.0 6 26 250 34 0.0 6 34 251 8 0.0 6 7 1 252 10 0.0 6 10 253 31 0.0 6 30 1 254 8 0.0 6 8 255 3 0.0 6 3 256 5 0.0 6 5 257 2 0.0 6 2 258 2 0.0 6 2 264 1 0.0 6 1 278 1 0.0 6 1 279 1 0.0 6 1 280 11 0.0 6 11 281 36 0.0 6 35 1 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 318 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 1 0.0 6 1 65 1 0.0 6 1 70 11 0.0 6 11 72 4 0.0 6 4 73 10 0.0 6 10 74 2 0.0 6 2 75 7 0.0 6 7 76 7 0.0 6 7 89 4 0.0 6 4 90 15 0.0 6 15 91 2 0.0 6 2 93 2 0.0 6 2 94 3 0.0 6 3 95 7 0.0 6 7 97 4 0.0 6 4 98 11 0.0 6 11 100 4 0.0 6 4 101 8 0.0 6 8 102 8 0.0 6 8 103 33 0.0 6 33 104 1 0.0 6 1 107 3 0.0 6 3 108 11 0.0 6 11 109 2 0.0 6 2 111 3 0.0 6 3 112 17 0.0 6 17 129 1 0.0 6 1 130 1 0.0 6 1 131 1 0.0 6 1 132 10 0.0 6 10 134 1 0.0 6 1 135 2 0.0 6 2 136 9 0.0 6 9 137 32 0.0 6 32 141 1 0.0 6 1 142 4 0.0 6 4 143 12 0.0 6 12 144 4 0.0 6 4 145 24 0.0 6 24 152 1 0.0 6 1 153 3 0.0 6 3 154 11 0.0 6 11 155 2 0.0 6 2 172 1 0.0 6 1 179 2 0.0 6 2 180 5 0.0 6 5 181 1 0.0 6 1 182 1 0.0 6 1 217 3 0.0 6 3 218 2 0.0 6 2 237 1 0.0 6 1 238 1 0.0 6 1 250 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_17_104_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_17_331_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_17_104_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_17_331_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.58 s (96 µs/read; 0.62 M reads/minute). === Summary === Total read pairs processed: 57,965 Read 1 with adapter: 57,963 (100.0%) Read 2 with adapter: 57,937 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 30 (0.1%) Pairs written (passing filters): 57,935 (99.9%) Total basepairs processed: 29,216,221 bp Read 1: 16,723,469 bp Read 2: 12,492,752 bp Total written (filtered): 3,477,473 bp (11.9%) Read 1: 1,796,519 bp Read 2: 1,680,954 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 57940 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 905.7 0 0 1 4 1 226.4 1 1 7 2 3.5 2 1 0 1 19 1 0.0 5 0 1 20 9 0.0 6 8 1 21 7 0.0 6 7 22 4 0.0 6 3 1 23 4 0.0 6 4 24 2 0.0 6 2 25 8 0.0 6 8 26 4 0.0 6 4 27 1 0.0 6 1 28 4 0.0 6 3 1 29 2 0.0 6 2 30 2 0.0 6 2 33 3 0.0 6 3 34 4 0.0 6 3 0 1 35 1 0.0 6 1 36 2 0.0 6 2 38 7 0.0 6 7 40 3 0.0 6 3 41 1 0.0 6 1 42 2 0.0 6 2 44 3 0.0 6 3 45 3 0.0 6 3 46 2 0.0 6 2 48 1 0.0 6 1 49 1 0.0 6 0 0 1 50 2 0.0 6 2 51 1 0.0 6 1 52 2 0.0 6 2 53 3 0.0 6 3 55 1 0.0 6 1 56 2 0.0 6 2 57 4 0.0 6 4 59 1 0.0 6 0 1 60 3 0.0 6 2 1 61 2 0.0 6 1 1 62 3 0.0 6 3 63 3 0.0 6 3 64 1 0.0 6 1 65 4 0.0 6 4 66 1 0.0 6 1 67 5 0.0 6 5 68 2 0.0 6 2 69 2 0.0 6 2 71 1 0.0 6 1 73 2 0.0 6 2 74 3 0.0 6 3 75 1 0.0 6 1 76 1 0.0 6 1 77 3 0.0 6 2 1 81 1 0.0 6 1 82 2 0.0 6 2 85 1 0.0 6 1 86 7 0.0 6 7 87 2 0.0 6 2 89 2 0.0 6 1 1 90 5 0.0 6 5 91 2 0.0 6 2 92 1 0.0 6 1 93 3 0.0 6 3 95 1 0.0 6 1 97 3 0.0 6 2 1 98 4 0.0 6 3 0 1 99 1 0.0 6 1 100 3 0.0 6 3 101 10 0.0 6 10 102 14 0.0 6 12 2 103 3 0.0 6 3 104 5 0.0 6 5 105 2 0.0 6 2 106 4 0.0 6 4 107 8 0.0 6 6 2 108 9 0.0 6 9 109 2 0.0 6 2 110 1 0.0 6 1 111 3 0.0 6 3 112 11 0.0 6 11 113 12 0.0 6 11 1 114 3 0.0 6 2 1 116 3 0.0 6 3 117 3 0.0 6 2 1 118 4 0.0 6 4 119 4 0.0 6 3 1 120 1 0.0 6 1 121 12 0.0 6 11 0 1 122 10 0.0 6 10 123 17 0.0 6 15 2 124 5 0.0 6 5 125 6 0.0 6 5 1 126 7 0.0 6 7 127 12 0.0 6 11 1 128 11 0.0 6 11 129 3 0.0 6 3 130 9 0.0 6 9 131 6 0.0 6 6 132 13 0.0 6 12 1 133 5 0.0 6 5 134 16 0.0 6 15 1 135 20 0.0 6 18 2 136 9 0.0 6 9 137 14 0.0 6 12 2 138 19 0.0 6 18 1 139 13 0.0 6 11 2 140 70 0.0 6 70 141 34 0.0 6 34 142 36 0.0 6 36 143 25 0.0 6 25 144 19 0.0 6 18 1 145 28 0.0 6 28 146 40 0.0 6 39 1 147 28 0.0 6 28 148 31 0.0 6 30 1 149 22 0.0 6 22 150 33 0.0 6 33 151 27 0.0 6 26 0 1 152 19 0.0 6 19 153 21 0.0 6 21 154 38 0.0 6 38 155 20 0.0 6 20 156 12 0.0 6 12 157 27 0.0 6 26 1 158 17 0.0 6 16 1 159 20 0.0 6 19 1 160 17 0.0 6 16 1 161 46 0.0 6 45 1 162 35 0.0 6 34 1 163 31 0.0 6 31 164 20 0.0 6 19 1 165 15 0.0 6 15 166 27 0.0 6 25 2 167 22 0.0 6 22 168 27 0.0 6 26 1 169 49 0.0 6 48 1 170 54 0.0 6 52 2 171 62 0.0 6 61 1 172 61 0.0 6 59 2 173 39 0.0 6 36 2 1 174 46 0.0 6 45 1 175 31 0.0 6 31 176 48 0.0 6 48 177 35 0.0 6 35 178 33 0.0 6 32 0 0 1 179 61 0.0 6 59 2 180 53 0.0 6 52 1 181 62 0.0 6 60 2 182 84 0.0 6 81 3 183 81 0.0 6 79 1 0 1 184 62 0.0 6 61 1 185 106 0.0 6 106 186 102 0.0 6 100 2 187 98 0.0 6 97 1 188 113 0.0 6 109 4 189 70 0.0 6 69 1 190 108 0.0 6 102 5 1 191 114 0.0 6 111 2 1 192 114 0.0 6 111 3 193 123 0.0 6 119 4 194 132 0.0 6 131 1 195 157 0.0 6 156 1 196 173 0.0 6 167 6 197 165 0.0 6 162 3 198 226 0.0 6 223 3 199 196 0.0 6 189 6 0 0 0 1 200 219 0.0 6 209 10 201 160 0.0 6 157 3 202 229 0.0 6 224 4 0 1 203 176 0.0 6 170 6 204 209 0.0 6 201 8 205 229 0.0 6 227 1 1 206 231 0.0 6 224 7 207 222 0.0 6 216 6 208 181 0.0 6 175 6 209 209 0.0 6 205 4 210 127 0.0 6 124 3 211 122 0.0 6 122 212 171 0.0 6 163 8 213 196 0.0 6 190 6 214 245 0.0 6 237 7 1 215 161 0.0 6 154 7 216 230 0.0 6 226 4 217 198 0.0 6 195 3 218 174 0.0 6 169 3 1 1 219 223 0.0 6 219 3 1 220 183 0.0 6 177 6 221 191 0.0 6 186 5 222 195 0.0 6 189 6 223 353 0.0 6 349 4 224 251 0.0 6 247 4 225 333 0.0 6 325 8 226 323 0.0 6 316 7 227 243 0.0 6 238 5 228 208 0.0 6 204 4 229 191 0.0 6 184 6 1 230 243 0.0 6 237 6 231 187 0.0 6 184 3 232 148 0.0 6 147 1 233 148 0.0 6 144 4 234 162 0.0 6 158 3 1 235 148 0.0 6 143 5 236 174 0.0 6 169 5 237 364 0.0 6 355 9 238 391 0.0 6 377 13 0 1 239 344 0.0 6 335 9 240 446 0.0 6 434 12 241 319 0.0 6 307 10 2 242 432 0.0 6 418 14 243 255 0.0 6 248 7 244 336 0.0 6 327 9 245 259 0.0 6 252 6 1 246 367 0.0 6 352 14 0 1 247 323 0.0 6 315 8 248 316 0.0 6 309 7 249 384 0.0 6 368 15 1 250 338 0.0 6 329 9 251 444 0.0 6 436 7 1 252 414 0.0 6 407 7 253 297 0.0 6 291 5 1 254 246 0.0 6 240 5 0 1 255 366 0.0 6 356 10 256 495 0.0 6 485 10 257 452 0.0 6 441 11 258 400 0.0 6 386 13 0 1 259 560 0.0 6 545 15 260 584 0.0 6 566 18 261 447 0.0 6 427 18 1 0 0 1 262 258 0.0 6 252 6 263 337 0.0 6 332 5 264 309 0.0 6 304 5 265 203 0.0 6 197 6 266 429 0.0 6 421 8 267 379 0.0 6 364 14 1 268 317 0.0 6 307 10 269 731 0.0 6 718 12 1 270 440 0.0 6 421 19 271 436 0.0 6 430 6 272 310 0.0 6 300 10 273 471 0.0 6 460 11 274 779 0.0 6 765 14 275 568 0.0 6 549 17 2 276 1185 0.0 6 1158 26 1 277 1449 0.0 6 1402 45 2 278 2058 0.0 6 1999 57 2 279 11770 0.0 6 11483 280 6 1 280 14124 0.0 6 13867 254 2 0 1 281 57 0.0 6 53 4 282 4 0.0 6 4 283 1 0.0 6 1 284 2 0.0 6 1 1 288 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 23 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 1 0.0 6 1 71 1 0.0 6 1 81 1 0.0 6 1 82 1 0.0 6 1 83 2 0.0 6 2 92 1 0.0 6 1 103 1 0.0 6 1 113 1 0.0 6 1 122 3 0.0 6 3 123 1 0.0 6 1 141 1 0.0 6 1 171 1 0.0 6 1 213 1 0.0 6 1 218 1 0.0 6 1 250 2 0.0 6 2 269 3 0.0 6 3 270 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 57910 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 226.4 1 0 1 6 1 14.2 1 1 7 3 3.5 2 2 1 10 1 0.1 3 1 11 1 0.0 3 1 12 3 0.0 3 3 13 5 0.0 3 4 1 14 2 0.0 4 2 15 1 0.0 4 0 1 16 7 0.0 4 5 1 1 17 13 0.0 5 13 18 2 0.0 5 2 19 32 0.0 5 27 5 20 68 0.0 6 64 4 21 40 0.0 6 39 1 22 31 0.0 6 28 3 23 20 0.0 6 18 2 24 30 0.0 6 29 1 25 12 0.0 6 10 2 26 12 0.0 6 12 27 12 0.0 6 12 28 3 0.0 6 3 29 11 0.0 6 9 1 0 0 1 30 5 0.0 6 5 31 6 0.0 6 6 32 11 0.0 6 11 33 8 0.0 6 8 34 6 0.0 6 4 2 35 5 0.0 6 5 36 11 0.0 6 11 37 7 0.0 6 6 1 38 16 0.0 6 16 39 8 0.0 6 8 40 11 0.0 6 10 1 41 9 0.0 6 9 42 3 0.0 6 3 43 10 0.0 6 10 44 13 0.0 6 12 1 45 6 0.0 6 5 0 0 0 1 46 17 0.0 6 16 1 47 13 0.0 6 10 3 48 11 0.0 6 9 1 0 0 1 49 21 0.0 6 17 3 0 0 1 50 19 0.0 6 17 2 51 9 0.0 6 6 1 1 0 1 52 19 0.0 6 17 2 53 13 0.0 6 12 1 54 4 0.0 6 4 55 29 0.0 6 25 4 56 23 0.0 6 21 2 57 28 0.0 6 26 2 58 12 0.0 6 11 1 59 14 0.0 6 11 2 1 60 26 0.0 6 23 2 0 0 1 61 12 0.0 6 12 62 14 0.0 6 13 1 63 12 0.0 6 11 1 64 9 0.0 6 9 65 5 0.0 6 4 0 0 0 0 1 66 14 0.0 6 14 67 13 0.0 6 12 1 68 15 0.0 6 15 69 40 0.0 6 33 6 1 70 93 0.0 6 86 7 71 31 0.0 6 29 2 72 66 0.0 6 63 2 1 73 10 0.0 6 10 74 26 0.0 6 24 2 75 13 0.0 6 12 1 76 11 0.0 6 10 1 77 22 0.0 6 20 1 0 0 1 78 28 0.0 6 27 1 79 11 0.0 6 10 0 1 80 23 0.0 6 21 1 0 0 1 81 19 0.0 6 16 3 82 18 0.0 6 17 0 1 83 47 0.0 6 42 5 84 39 0.0 6 38 0 0 0 1 85 35 0.0 6 33 2 86 31 0.0 6 29 2 87 53 0.0 6 48 4 1 88 17 0.0 6 17 89 14 0.0 6 11 3 90 33 0.0 6 30 3 91 32 0.0 6 31 1 92 33 0.0 6 31 2 93 34 0.0 6 32 1 1 94 92 0.0 6 87 5 95 30 0.0 6 28 1 1 96 43 0.0 6 34 9 97 27 0.0 6 25 2 98 33 0.0 6 31 1 0 1 99 71 0.0 6 65 5 0 1 100 35 0.0 6 32 2 1 101 41 0.0 6 34 7 102 61 0.0 6 55 4 1 0 1 103 58 0.0 6 51 6 0 0 1 104 58 0.0 6 57 1 105 66 0.0 6 61 4 0 0 1 106 35 0.0 6 32 3 107 68 0.0 6 57 11 108 47 0.0 6 43 2 1 1 109 32 0.0 6 27 5 110 36 0.0 6 32 3 1 111 50 0.0 6 48 2 112 53 0.0 6 51 2 113 39 0.0 6 37 2 114 52 0.0 6 51 1 115 52 0.0 6 48 3 0 0 0 0 1 116 41 0.0 6 39 1 1 117 32 0.0 6 28 4 118 36 0.0 6 35 1 119 56 0.0 6 56 120 78 0.0 6 72 6 121 47 0.0 6 46 1 122 66 0.0 6 62 2 2 123 74 0.0 6 69 5 124 61 0.0 6 60 1 125 37 0.0 6 36 1 126 36 0.0 6 35 1 127 50 0.0 6 47 3 128 37 0.0 6 36 1 129 54 0.0 6 52 1 0 0 1 130 50 0.0 6 46 3 0 1 131 69 0.0 6 66 3 132 89 0.0 6 84 5 133 48 0.0 6 47 1 134 109 0.0 6 104 4 1 135 229 0.0 6 216 10 0 1 2 136 135 0.0 6 128 6 0 1 137 159 0.0 6 155 4 138 99 0.0 6 98 1 139 159 0.0 6 154 5 140 156 0.0 6 151 5 141 190 0.0 6 182 8 142 239 0.0 6 234 5 143 195 0.0 6 189 6 144 309 0.0 6 295 14 145 161 0.0 6 153 8 146 161 0.0 6 156 5 147 119 0.0 6 112 5 1 1 148 143 0.0 6 139 4 149 257 0.0 6 250 7 150 316 0.0 6 303 13 151 218 0.0 6 209 9 152 433 0.0 6 418 14 0 0 1 153 412 0.0 6 400 12 154 312 0.0 6 300 10 1 0 1 155 369 0.0 6 354 14 0 1 156 309 0.0 6 300 8 0 0 1 157 347 0.0 6 339 6 0 1 0 0 1 158 413 0.0 6 395 16 0 2 159 215 0.0 6 211 4 160 402 0.0 6 392 8 1 0 1 161 219 0.0 6 209 9 0 0 1 162 217 0.0 6 207 10 163 371 0.0 6 357 12 1 0 1 164 324 0.0 6 312 12 165 427 0.0 6 418 9 166 476 0.0 6 455 21 167 272 0.0 6 267 5 168 430 0.0 6 418 11 1 169 195 0.0 6 192 3 170 259 0.0 6 248 9 0 1 0 1 171 388 0.0 6 378 9 1 172 203 0.0 6 193 9 1 173 364 0.0 6 348 13 2 1 174 468 0.0 6 447 21 175 920 0.0 6 892 26 1 0 1 176 525 0.0 6 512 11 2 177 553 0.0 6 531 22 178 500 0.0 6 481 18 1 179 547 0.0 6 532 15 180 576 0.0 6 556 18 2 181 716 0.0 6 696 18 2 182 453 0.0 6 443 10 183 728 0.0 6 712 14 1 1 184 196 0.0 6 189 6 1 185 218 0.0 6 208 9 0 1 186 755 0.0 6 728 24 3 187 1044 0.0 6 1011 31 1 0 1 188 1309 0.0 6 1277 30 2 189 924 0.0 6 899 24 1 190 1566 0.0 6 1528 37 0 0 1 191 1195 0.0 6 1171 24 192 889 0.0 6 866 21 2 193 641 0.0 6 626 12 3 194 944 0.0 6 917 25 1 1 195 1016 0.0 6 977 38 0 0 1 196 2433 0.0 6 2393 39 1 197 751 0.0 6 729 22 198 1604 0.0 6 1551 49 1 1 1 1 199 2718 0.0 6 2646 67 2 0 1 2 200 1083 0.0 6 1056 24 1 0 2 201 1381 0.0 6 1355 26 202 851 0.0 6 829 21 0 0 1 203 716 0.0 6 693 23 204 741 0.0 6 724 17 205 422 0.0 6 408 13 0 0 1 206 398 0.0 6 387 11 207 287 0.0 6 281 6 208 474 0.0 6 461 12 0 1 209 321 0.0 6 316 5 210 361 0.0 6 353 7 0 0 0 0 1 211 535 0.0 6 521 13 0 0 1 212 470 0.0 6 459 11 213 152 0.0 6 147 2 1 1 1 214 322 0.0 6 314 6 1 1 215 343 0.0 6 335 8 216 465 0.0 6 450 14 1 217 855 0.0 6 836 19 218 460 0.0 6 443 16 0 0 0 1 219 556 0.0 6 543 13 220 364 0.0 6 357 6 0 0 1 221 629 0.0 6 617 11 1 222 249 0.0 6 240 8 1 223 446 0.0 6 435 11 224 434 0.0 6 420 14 225 545 0.0 6 530 15 226 503 0.0 6 494 8 0 0 1 227 494 0.0 6 488 6 228 281 0.0 6 277 4 229 527 0.0 6 512 14 1 230 372 0.0 6 366 6 231 384 0.0 6 371 12 0 1 232 450 0.0 6 432 16 0 1 1 233 362 0.0 6 350 12 234 205 0.0 6 201 4 235 185 0.0 6 179 6 236 80 0.0 6 77 3 237 114 0.0 6 109 5 238 57 0.0 6 56 1 239 45 0.0 6 45 240 83 0.0 6 83 241 169 0.0 6 168 1 242 162 0.0 6 161 1 243 91 0.0 6 90 1 244 236 0.0 6 234 2 245 144 0.0 6 142 2 246 72 0.0 6 70 2 247 62 0.0 6 60 2 248 49 0.0 6 49 249 59 0.0 6 57 1 1 250 44 0.0 6 42 2 251 88 0.0 6 87 1 252 26 0.0 6 26 253 14 0.0 6 14 254 63 0.0 6 61 1 1 255 10 0.0 6 10 256 13 0.0 6 13 257 6 0.0 6 6 258 5 0.0 6 5 266 1 0.0 6 1 280 3 0.0 6 3 281 27 0.0 6 26 1 282 46 0.0 6 44 1 1 286 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 27 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 2 0.0 6 2 74 1 0.0 6 1 83 1 0.0 6 1 92 1 0.0 6 1 103 1 0.0 6 1 108 2 0.0 6 2 112 2 0.0 6 2 124 1 0.0 6 1 135 2 0.0 6 2 137 2 0.0 6 2 141 1 0.0 6 1 147 1 0.0 6 1 153 2 0.0 6 2 154 1 0.0 6 1 158 1 0.0 6 1 159 1 0.0 6 1 162 1 0.0 6 1 167 1 0.0 6 1 172 1 0.0 6 1 177 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_18_105_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_18_332_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_18_105_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_18_332_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.26 s (138 µs/read; 0.43 M reads/minute). === Summary === Total read pairs processed: 9,083 Read 1 with adapter: 9,081 (100.0%) Read 2 with adapter: 9,076 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 9 (0.1%) Pairs written (passing filters): 9,074 (99.9%) Total basepairs processed: 4,097,894 bp Read 1: 2,243,233 bp Read 2: 1,854,661 bp Total written (filtered): 596,058 bp (14.5%) Read 1: 293,014 bp Read 2: 303,044 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 8911 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.8% G: 0.0% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 35.5 1 1 5 1 8.9 1 1 9 1 0.0 2 1 19 2 0.0 5 0 2 20 11 0.0 6 11 21 4 0.0 6 3 1 22 2 0.0 6 2 23 1 0.0 6 1 24 3 0.0 6 3 25 4 0.0 6 4 31 2 0.0 6 2 32 1 0.0 6 1 33 2 0.0 6 1 1 35 1 0.0 6 1 39 1 0.0 6 1 40 2 0.0 6 2 41 4 0.0 6 4 43 2 0.0 6 2 45 2 0.0 6 2 46 1 0.0 6 1 48 1 0.0 6 1 49 1 0.0 6 1 61 1 0.0 6 1 65 1 0.0 6 1 66 1 0.0 6 1 70 1 0.0 6 1 73 2 0.0 6 2 75 2 0.0 6 2 76 2 0.0 6 1 1 77 1 0.0 6 1 78 1 0.0 6 1 79 1 0.0 6 1 81 1 0.0 6 1 83 1 0.0 6 1 86 1 0.0 6 1 89 1 0.0 6 1 90 4 0.0 6 4 93 1 0.0 6 1 94 1 0.0 6 1 95 1 0.0 6 1 96 1 0.0 6 1 98 3 0.0 6 3 100 1 0.0 6 1 101 3 0.0 6 3 102 1 0.0 6 1 103 4 0.0 6 4 104 5 0.0 6 5 105 1 0.0 6 1 107 1 0.0 6 0 1 108 2 0.0 6 2 109 9 0.0 6 9 110 1 0.0 6 1 111 4 0.0 6 4 112 10 0.0 6 10 113 8 0.0 6 8 114 4 0.0 6 4 115 4 0.0 6 4 116 8 0.0 6 8 117 1 0.0 6 1 120 1 0.0 6 1 121 3 0.0 6 3 122 3 0.0 6 3 123 2 0.0 6 2 125 1 0.0 6 1 126 1 0.0 6 1 127 5 0.0 6 5 128 5 0.0 6 5 129 3 0.0 6 3 130 4 0.0 6 3 1 131 6 0.0 6 5 1 132 5 0.0 6 5 133 11 0.0 6 11 134 10 0.0 6 10 135 7 0.0 6 7 136 9 0.0 6 9 137 7 0.0 6 7 138 5 0.0 6 5 139 6 0.0 6 6 140 12 0.0 6 12 141 19 0.0 6 18 1 142 18 0.0 6 17 1 143 31 0.0 6 31 144 69 0.0 6 69 145 540 0.0 6 538 2 146 901 0.0 6 899 2 147 152 0.0 6 150 2 148 103 0.0 6 102 1 149 912 0.0 6 910 2 150 128 0.0 6 128 151 3 0.0 6 3 152 2 0.0 6 1 1 153 19 0.0 6 18 1 154 8 0.0 6 8 155 1 0.0 6 1 156 1 0.0 6 1 157 3 0.0 6 2 1 158 7 0.0 6 6 1 159 43 0.0 6 43 160 8 0.0 6 8 161 2 0.0 6 2 162 3 0.0 6 2 1 163 1 0.0 6 1 164 2 0.0 6 2 165 4 0.0 6 4 167 2 0.0 6 2 168 3 0.0 6 2 1 169 6 0.0 6 6 170 4 0.0 6 2 2 172 4 0.0 6 4 173 5 0.0 6 5 174 2 0.0 6 2 175 1 0.0 6 1 176 2 0.0 6 2 177 3 0.0 6 3 178 4 0.0 6 3 1 179 8 0.0 6 7 1 180 2 0.0 6 2 181 6 0.0 6 4 2 182 6 0.0 6 6 183 3 0.0 6 2 1 184 3 0.0 6 3 185 13 0.0 6 12 1 186 10 0.0 6 10 187 11 0.0 6 11 188 10 0.0 6 9 1 189 7 0.0 6 7 190 9 0.0 6 9 191 9 0.0 6 8 0 0 0 1 192 6 0.0 6 5 1 193 9 0.0 6 8 1 194 5 0.0 6 5 195 10 0.0 6 10 196 14 0.0 6 13 1 197 5 0.0 6 5 198 25 0.0 6 23 2 199 15 0.0 6 13 2 200 22 0.0 6 19 3 201 18 0.0 6 18 202 23 0.0 6 22 1 203 14 0.0 6 13 1 204 12 0.0 6 11 1 205 22 0.0 6 21 1 206 21 0.0 6 21 207 31 0.0 6 30 1 208 26 0.0 6 26 209 22 0.0 6 21 1 210 15 0.0 6 15 211 20 0.0 6 20 212 21 0.0 6 21 213 17 0.0 6 16 1 214 24 0.0 6 24 215 10 0.0 6 10 216 13 0.0 6 12 1 217 13 0.0 6 13 218 20 0.0 6 20 219 25 0.0 6 24 1 220 15 0.0 6 15 221 17 0.0 6 17 222 22 0.0 6 20 2 223 30 0.0 6 29 1 224 32 0.0 6 31 1 225 23 0.0 6 22 1 226 39 0.0 6 38 1 227 29 0.0 6 28 1 228 26 0.0 6 26 229 17 0.0 6 17 230 20 0.0 6 19 1 231 23 0.0 6 21 2 232 17 0.0 6 15 2 233 14 0.0 6 14 234 30 0.0 6 27 3 235 11 0.0 6 8 3 236 32 0.0 6 31 1 237 24 0.0 6 22 2 238 42 0.0 6 40 2 239 38 0.0 6 37 1 240 26 0.0 6 24 2 241 22 0.0 6 22 242 49 0.0 6 49 243 16 0.0 6 16 244 29 0.0 6 29 245 29 0.0 6 27 2 246 45 0.0 6 40 5 247 16 0.0 6 16 248 37 0.0 6 35 2 249 33 0.0 6 32 1 250 61 0.0 6 60 1 251 66 0.0 6 65 1 252 35 0.0 6 32 3 253 26 0.0 6 24 2 254 59 0.0 6 55 4 255 77 0.0 6 72 4 0 0 0 1 256 78 0.0 6 71 7 257 99 0.0 6 97 1 1 258 41 0.0 6 39 2 259 104 0.0 6 101 3 260 109 0.0 6 104 5 261 163 0.0 6 159 4 262 31 0.0 6 27 4 263 18 0.0 6 17 0 0 0 0 0 1 264 60 0.0 6 57 3 265 52 0.0 6 50 2 266 55 0.0 6 52 3 267 78 0.0 6 74 4 268 47 0.0 6 42 5 269 97 0.0 6 92 5 270 32 0.0 6 30 2 271 64 0.0 6 63 1 272 20 0.0 6 18 2 273 53 0.0 6 50 3 274 117 0.0 6 115 2 275 104 0.0 6 100 4 276 239 0.0 6 231 8 277 247 0.0 6 240 6 1 278 207 0.0 6 199 8 279 1685 0.0 6 1629 51 5 280 136 0.0 6 129 7 281 5 0.0 6 4 1 283 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 170 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 5 0.0 6 5 73 24 0.0 6 24 74 50 0.0 6 50 75 2 0.0 6 2 76 15 0.0 6 15 77 62 0.0 6 62 78 1 0.0 6 1 81 2 0.0 6 2 82 1 0.0 6 0 0 0 0 0 0 1 83 1 0.0 6 1 86 2 0.0 6 2 87 5 0.0 6 5 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 8868 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.8% G: 0.1% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 10 2 0.0 3 1 1 11 1 0.0 3 0 0 1 14 1 0.0 4 1 16 2 0.0 4 1 1 17 1 0.0 5 0 1 18 3 0.0 5 3 20 9 0.0 6 9 21 6 0.0 6 6 22 1 0.0 6 1 23 2 0.0 6 2 24 3 0.0 6 3 25 2 0.0 6 1 1 30 1 0.0 6 1 32 1 0.0 6 1 34 2 0.0 6 2 38 1 0.0 6 1 39 2 0.0 6 2 41 2 0.0 6 1 0 0 0 1 42 1 0.0 6 0 1 43 1 0.0 6 1 44 1 0.0 6 1 45 2 0.0 6 2 46 5 0.0 6 3 0 2 47 2 0.0 6 2 48 1 0.0 6 1 49 4 0.0 6 4 50 2 0.0 6 2 51 1 0.0 6 1 52 2 0.0 6 2 53 3 0.0 6 3 54 1 0.0 6 1 55 4 0.0 6 3 1 56 1 0.0 6 1 57 1 0.0 6 1 58 5 0.0 6 3 0 1 1 59 3 0.0 6 3 60 3 0.0 6 3 62 1 0.0 6 1 63 1 0.0 6 1 64 3 0.0 6 3 65 2 0.0 6 2 66 3 0.0 6 3 67 22 0.0 6 22 68 11 0.0 6 11 69 6 0.0 6 6 70 3 0.0 6 2 1 71 8 0.0 6 8 72 3 0.0 6 3 73 1 0.0 6 1 74 4 0.0 6 4 75 6 0.0 6 5 1 76 2 0.0 6 2 77 5 0.0 6 5 78 2 0.0 6 2 79 7 0.0 6 7 80 1 0.0 6 1 81 5 0.0 6 5 82 3 0.0 6 3 83 3 0.0 6 2 1 84 5 0.0 6 5 85 6 0.0 6 5 1 86 4 0.0 6 4 87 2 0.0 6 2 88 7 0.0 6 7 89 5 0.0 6 5 90 2 0.0 6 2 91 7 0.0 6 6 1 92 7 0.0 6 7 93 7 0.0 6 7 94 6 0.0 6 5 1 95 3 0.0 6 3 96 13 0.0 6 11 2 97 7 0.0 6 6 1 98 2 0.0 6 2 99 4 0.0 6 4 100 4 0.0 6 4 101 7 0.0 6 7 102 8 0.0 6 8 103 8 0.0 6 8 104 23 0.0 6 23 105 11 0.0 6 11 106 4 0.0 6 4 107 7 0.0 6 6 1 108 4 0.0 6 4 109 13 0.0 6 13 110 12 0.0 6 11 1 111 16 0.0 6 16 112 19 0.0 6 18 1 113 19 0.0 6 19 114 11 0.0 6 10 0 0 0 1 115 11 0.0 6 11 116 14 0.0 6 13 1 117 11 0.0 6 10 1 118 18 0.0 6 17 1 119 23 0.0 6 23 120 16 0.0 6 16 121 10 0.0 6 10 122 23 0.0 6 23 123 21 0.0 6 21 124 19 0.0 6 18 0 0 1 125 46 0.0 6 45 1 126 24 0.0 6 23 1 127 24 0.0 6 24 128 27 0.0 6 27 129 23 0.0 6 23 130 29 0.0 6 28 1 131 20 0.0 6 20 132 27 0.0 6 27 133 22 0.0 6 21 1 134 36 0.0 6 35 1 135 53 0.0 6 52 0 0 1 136 35 0.0 6 35 137 42 0.0 6 42 138 37 0.0 6 37 139 100 0.0 6 100 140 150 0.0 6 150 141 488 0.0 6 485 2 0 1 142 733 0.0 6 730 3 143 140 0.0 6 140 144 177 0.0 6 174 3 145 620 0.0 6 618 2 146 103 0.0 6 102 1 147 9 0.0 6 9 148 23 0.0 6 21 1 0 0 1 149 37 0.0 6 36 1 150 43 0.0 6 42 1 151 20 0.0 6 18 2 152 33 0.0 6 32 1 153 31 0.0 6 30 1 154 37 0.0 6 34 3 155 51 0.0 6 48 3 156 43 0.0 6 41 2 157 47 0.0 6 45 1 1 158 25 0.0 6 22 3 159 20 0.0 6 16 4 160 21 0.0 6 19 1 0 1 161 17 0.0 6 16 1 162 20 0.0 6 18 2 163 29 0.0 6 27 2 164 53 0.0 6 50 3 165 46 0.0 6 42 3 1 166 40 0.0 6 39 1 167 20 0.0 6 19 1 168 38 0.0 6 35 2 0 0 1 169 14 0.0 6 14 170 28 0.0 6 27 1 171 39 0.0 6 38 1 172 53 0.0 6 51 2 173 37 0.0 6 36 1 174 58 0.0 6 55 3 175 87 0.0 6 87 176 38 0.0 6 36 2 177 45 0.0 6 43 1 0 0 1 178 33 0.0 6 32 1 179 53 0.0 6 52 1 180 27 0.0 6 25 2 181 46 0.0 6 42 3 1 182 26 0.0 6 25 1 183 71 0.0 6 69 2 184 37 0.0 6 36 1 185 60 0.0 6 58 2 186 66 0.0 6 62 4 187 82 0.0 6 79 2 1 188 68 0.0 6 60 8 189 85 0.0 6 82 3 190 80 0.0 6 79 1 191 90 0.0 6 88 2 192 62 0.0 6 59 3 193 36 0.0 6 34 1 0 0 1 194 77 0.0 6 72 5 195 97 0.0 6 94 3 196 176 0.0 6 168 8 197 41 0.0 6 36 5 198 117 0.0 6 111 6 199 149 0.0 6 142 7 200 100 0.0 6 92 8 201 105 0.0 6 97 8 202 85 0.0 6 82 3 203 72 0.0 6 68 4 204 60 0.0 6 60 205 49 0.0 6 47 2 206 77 0.0 6 72 3 2 207 15 0.0 6 15 208 40 0.0 6 38 2 209 35 0.0 6 35 210 26 0.0 6 24 1 1 211 16 0.0 6 15 1 212 35 0.0 6 33 2 213 16 0.0 6 15 1 214 53 0.0 6 50 3 215 41 0.0 6 40 1 216 45 0.0 6 44 1 217 40 0.0 6 38 1 0 1 218 61 0.0 6 55 6 219 84 0.0 6 81 3 220 16 0.0 6 14 1 0 0 1 221 57 0.0 6 56 0 1 222 50 0.0 6 45 5 223 125 0.0 6 119 6 224 162 0.0 6 156 6 225 93 0.0 6 90 3 226 108 0.0 6 102 6 227 175 0.0 6 171 4 228 64 0.0 6 60 4 229 167 0.0 6 161 6 230 32 0.0 6 31 1 231 83 0.0 6 82 1 232 75 0.0 6 73 2 233 33 0.0 6 32 1 234 37 0.0 6 35 2 235 16 0.0 6 14 1 1 236 5 0.0 6 5 237 7 0.0 6 7 238 24 0.0 6 24 239 18 0.0 6 18 240 5 0.0 6 5 241 23 0.0 6 20 3 242 27 0.0 6 23 4 243 28 0.0 6 28 244 33 0.0 6 32 1 245 5 0.0 6 5 246 19 0.0 6 19 247 9 0.0 6 7 2 248 30 0.0 6 30 249 10 0.0 6 10 250 1 0.0 6 1 251 19 0.0 6 19 252 5 0.0 6 5 253 2 0.0 6 2 278 6 0.0 6 6 279 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 208 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 2 0.0 6 2 72 1 0.0 6 1 73 2 0.0 6 2 74 40 0.0 6 40 75 71 0.0 6 71 76 1 0.0 6 1 77 9 0.0 6 9 78 76 0.0 6 76 81 1 0.0 6 0 0 0 1 82 1 0.0 6 1 88 4 0.0 6 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_19_106_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_19_333_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_19_106_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_19_333_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.88 s (67 µs/read; 0.89 M reads/minute). === Summary === Total read pairs processed: 87,199 Read 1 with adapter: 87,183 (100.0%) Read 2 with adapter: 87,153 (99.9%) == Read fate breakdown == Pairs that were too short: 51 (0.1%) Pairs discarded as untrimmed: 62 (0.1%) Pairs written (passing filters): 87,086 (99.9%) Total basepairs processed: 36,484,252 bp Read 1: 20,086,465 bp Read 2: 16,397,787 bp Total written (filtered): 5,729,868 bp (15.7%) Read 1: 3,026,265 bp Read 2: 2,703,603 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 81813 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 5 1362.5 0 3 2 4 2 340.6 1 1 1 5 2 85.2 1 2 6 5 21.3 1 2 0 3 7 1 5.3 2 0 0 1 8 1 1.3 2 1 9 2 0.3 2 2 11 1 0.0 3 1 14 1 0.0 4 1 15 1 0.0 4 1 16 5 0.0 4 2 2 0 1 17 2 0.0 5 1 1 19 1 0.0 5 1 20 177 0.0 6 174 3 21 89 0.0 6 84 5 22 90 0.0 6 87 3 23 41 0.0 6 40 1 24 30 0.0 6 28 2 25 41 0.0 6 37 4 26 34 0.0 6 33 1 27 14 0.0 6 13 0 0 1 28 16 0.0 6 14 1 1 29 10 0.0 6 10 30 8 0.0 6 8 31 8 0.0 6 7 1 32 7 0.0 6 7 33 7 0.0 6 7 34 9 0.0 6 9 35 9 0.0 6 9 36 11 0.0 6 9 1 0 0 0 1 37 2 0.0 6 2 38 6 0.0 6 6 39 19 0.0 6 18 1 40 11 0.0 6 11 41 3 0.0 6 3 42 3 0.0 6 3 43 10 0.0 6 10 44 6 0.0 6 6 45 17 0.0 6 14 3 46 3 0.0 6 3 47 5 0.0 6 5 48 10 0.0 6 10 49 4 0.0 6 4 50 1 0.0 6 1 51 11 0.0 6 10 1 52 6 0.0 6 5 1 53 8 0.0 6 8 54 2 0.0 6 2 55 3 0.0 6 2 1 56 4 0.0 6 3 1 57 5 0.0 6 5 58 6 0.0 6 6 59 11 0.0 6 10 1 60 3 0.0 6 3 61 2 0.0 6 2 62 3 0.0 6 3 63 15 0.0 6 14 1 64 8 0.0 6 8 65 9 0.0 6 8 1 66 8 0.0 6 7 1 67 9 0.0 6 9 68 8 0.0 6 8 69 6 0.0 6 6 70 3 0.0 6 3 71 13 0.0 6 12 1 72 12 0.0 6 12 73 6 0.0 6 6 74 13 0.0 6 13 75 7 0.0 6 7 76 10 0.0 6 10 77 10 0.0 6 10 78 8 0.0 6 8 79 13 0.0 6 13 80 9 0.0 6 9 81 11 0.0 6 11 82 7 0.0 6 7 83 11 0.0 6 11 84 17 0.0 6 17 85 10 0.0 6 10 86 12 0.0 6 12 87 12 0.0 6 12 88 12 0.0 6 11 1 89 10 0.0 6 10 90 8 0.0 6 8 91 10 0.0 6 9 1 92 15 0.0 6 15 93 7 0.0 6 7 94 4 0.0 6 4 95 8 0.0 6 8 96 7 0.0 6 7 97 9 0.0 6 9 98 12 0.0 6 12 99 15 0.0 6 15 100 17 0.0 6 17 101 15 0.0 6 14 1 102 19 0.0 6 18 1 103 29 0.0 6 28 1 104 18 0.0 6 18 105 18 0.0 6 17 1 106 23 0.0 6 23 107 22 0.0 6 22 108 20 0.0 6 19 1 109 19 0.0 6 19 110 21 0.0 6 21 111 18 0.0 6 18 112 25 0.0 6 24 0 1 113 21 0.0 6 21 114 20 0.0 6 20 115 16 0.0 6 16 116 36 0.0 6 34 0 2 117 19 0.0 6 19 118 20 0.0 6 18 2 119 22 0.0 6 21 0 1 120 20 0.0 6 20 121 25 0.0 6 25 122 21 0.0 6 21 123 31 0.0 6 30 1 124 22 0.0 6 22 125 26 0.0 6 25 1 126 37 0.0 6 37 127 30 0.0 6 30 128 24 0.0 6 24 129 48 0.0 6 48 130 44 0.0 6 43 0 1 131 44 0.0 6 43 1 132 38 0.0 6 38 133 42 0.0 6 42 134 38 0.0 6 38 135 47 0.0 6 46 1 136 58 0.0 6 58 137 52 0.0 6 51 1 138 69 0.0 6 68 1 139 58 0.0 6 58 140 127 0.0 6 126 1 141 525 0.0 6 523 2 142 1502 0.0 6 1499 3 143 573 0.0 6 573 144 700 0.0 6 698 2 145 536 0.0 6 535 1 146 1048 0.0 6 1046 2 147 650 0.0 6 647 3 148 496 0.0 6 495 0 1 149 746 0.0 6 746 150 511 0.0 6 510 1 151 922 0.0 6 921 1 152 281 0.0 6 281 153 327 0.0 6 325 2 154 324 0.0 6 322 1 0 1 155 356 0.0 6 355 1 156 268 0.0 6 267 0 0 1 157 228 0.0 6 226 2 158 900 0.0 6 896 4 159 601 0.0 6 600 1 160 355 0.0 6 355 161 307 0.0 6 305 0 1 0 1 162 319 0.0 6 317 2 163 703 0.0 6 700 3 164 633 0.0 6 631 1 1 165 670 0.0 6 667 3 166 200 0.0 6 200 167 316 0.0 6 316 168 383 0.0 6 382 1 169 997 0.0 6 995 2 170 676 0.0 6 672 4 171 390 0.0 6 386 2 0 0 1 1 172 310 0.0 6 307 1 0 1 1 173 989 0.0 6 989 174 390 0.0 6 388 2 175 348 0.0 6 346 2 176 450 0.0 6 448 1 0 0 1 177 286 0.0 6 284 2 178 651 0.0 6 647 2 0 0 1 1 179 814 0.0 6 811 3 180 734 0.0 6 733 1 181 354 0.0 6 352 2 182 753 0.0 6 752 1 183 549 0.0 6 544 4 1 184 345 0.0 6 342 3 185 481 0.0 6 479 2 186 608 0.0 6 606 2 187 362 0.0 6 358 3 0 0 0 1 188 328 0.0 6 325 2 0 1 189 421 0.0 6 420 1 190 518 0.0 6 516 2 191 317 0.0 6 314 2 0 0 0 0 1 192 481 0.0 6 479 2 193 452 0.0 6 452 194 746 0.0 6 746 195 777 0.0 6 773 4 196 769 0.0 6 765 1 2 0 0 1 197 838 0.0 6 834 3 0 0 0 1 198 920 0.0 6 916 1 0 0 1 2 199 772 0.0 6 763 9 200 1079 0.0 6 1076 3 201 605 0.0 6 603 2 202 693 0.0 6 688 3 0 0 1 0 1 203 1221 0.0 6 1218 3 204 1117 0.0 6 1115 2 205 581 0.0 6 575 5 0 1 206 727 0.0 6 720 6 0 0 0 1 207 1158 0.0 6 1150 7 0 0 0 0 1 208 786 0.0 6 780 5 0 1 209 1186 0.0 6 1184 2 210 731 0.0 6 727 4 211 679 0.0 6 673 6 212 650 0.0 6 648 2 213 578 0.0 6 572 6 214 760 0.0 6 756 3 0 0 0 1 215 901 0.0 6 894 7 216 1087 0.0 6 1080 5 1 0 0 1 217 581 0.0 6 578 3 218 747 0.0 6 743 2 1 0 0 0 1 219 946 0.0 6 940 6 220 1128 0.0 6 1125 1 0 1 0 0 1 221 1066 0.0 6 1064 2 222 577 0.0 6 570 4 1 0 0 0 2 223 711 0.0 6 708 2 0 1 224 726 0.0 6 722 4 225 1159 0.0 6 1152 6 0 0 0 0 1 226 1070 0.0 6 1065 5 227 1384 0.0 6 1381 3 228 822 0.0 6 819 2 1 229 476 0.0 6 474 2 230 571 0.0 6 569 2 231 632 0.0 6 628 3 1 232 1038 0.0 6 1030 8 233 421 0.0 6 417 3 1 234 491 0.0 6 488 2 1 235 464 0.0 6 461 3 236 410 0.0 6 409 1 237 535 0.0 6 530 5 238 427 0.0 6 423 3 0 0 0 1 239 481 0.0 6 476 5 240 501 0.0 6 494 7 241 695 0.0 6 693 2 242 500 0.0 6 498 2 243 493 0.0 6 489 4 244 672 0.0 6 666 6 245 424 0.0 6 421 3 246 279 0.0 6 279 247 295 0.0 6 291 3 1 248 341 0.0 6 339 2 249 426 0.0 6 423 3 250 336 0.0 6 335 0 0 1 251 285 0.0 6 284 1 252 327 0.0 6 324 3 253 344 0.0 6 338 6 254 383 0.0 6 379 3 0 0 0 0 1 255 314 0.0 6 308 6 256 336 0.0 6 334 2 257 286 0.0 6 282 3 0 1 258 300 0.0 6 295 5 259 282 0.0 6 280 1 0 0 0 0 1 260 340 0.0 6 336 4 261 342 0.0 6 337 5 262 215 0.0 6 214 1 263 138 0.0 6 136 2 264 161 0.0 6 161 265 220 0.0 6 219 1 266 136 0.0 6 136 267 160 0.0 6 159 1 268 126 0.0 6 124 2 269 169 0.0 6 167 2 270 162 0.0 6 161 1 271 126 0.0 6 126 272 115 0.0 6 114 1 273 117 0.0 6 113 2 1 0 0 1 274 212 0.0 6 206 6 275 208 0.0 6 206 2 276 346 0.0 6 338 8 277 394 0.0 6 382 10 2 278 441 0.0 6 434 6 1 279 1544 0.0 6 1506 38 280 1226 0.0 6 1217 9 281 9 0.0 6 8 0 1 283 1 0.0 6 1 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 5370 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 47 1 0.0 6 1 48 3 0.0 6 1 0 0 2 49 3 0.0 6 1 1 1 50 29 0.0 6 12 15 2 51 2581 0.0 6 2493 87 0 1 52 5 0.0 6 3 2 57 1 0.0 6 0 0 0 0 0 0 1 63 1 0.0 6 1 68 1 0.0 6 1 70 9 0.0 6 8 1 71 33 0.0 6 33 72 77 0.0 6 77 73 28 0.0 6 28 74 30 0.0 6 30 75 31 0.0 6 31 76 43 0.0 6 42 1 77 26 0.0 6 25 1 78 29 0.0 6 29 79 27 0.0 6 27 80 18 0.0 6 18 81 34 0.0 6 34 82 3 0.0 6 3 83 9 0.0 6 9 84 21 0.0 6 21 85 8 0.0 6 8 86 15 0.0 6 15 87 19 0.0 6 19 88 33 0.0 6 33 89 19 0.0 6 19 90 11 0.0 6 11 91 10 0.0 6 10 92 13 0.0 6 12 1 93 28 0.0 6 28 94 32 0.0 6 31 1 95 26 0.0 6 26 96 5 0.0 6 5 97 6 0.0 6 6 98 13 0.0 6 13 99 29 0.0 6 29 100 31 0.0 6 31 101 7 0.0 6 7 102 16 0.0 6 16 103 39 0.0 6 39 104 10 0.0 6 10 105 4 0.0 6 4 106 19 0.0 6 19 107 7 0.0 6 7 108 37 0.0 6 37 109 34 0.0 6 34 110 30 0.0 6 30 111 7 0.0 6 7 112 25 0.0 6 25 113 10 0.0 6 10 114 14 0.0 6 14 115 15 0.0 6 15 116 19 0.0 6 19 117 2 0.0 6 2 118 5 0.0 6 5 119 13 0.0 6 13 120 7 0.0 6 7 122 6 0.0 6 5 0 0 0 0 0 1 123 13 0.0 6 13 124 20 0.0 6 20 125 9 0.0 6 9 126 21 0.0 6 21 127 17 0.0 6 17 128 18 0.0 6 18 129 13 0.0 6 13 130 21 0.0 6 21 131 7 0.0 6 7 132 16 0.0 6 16 133 63 0.0 6 63 134 31 0.0 6 31 135 4 0.0 6 4 136 16 0.0 6 16 137 29 0.0 6 29 138 21 0.0 6 21 139 38 0.0 6 38 140 22 0.0 6 22 141 10 0.0 6 10 142 4 0.0 6 4 143 8 0.0 6 8 144 23 0.0 6 23 145 8 0.0 6 8 146 18 0.0 6 18 147 1 0.0 6 1 148 1 0.0 6 1 149 5 0.0 6 5 150 24 0.0 6 24 151 13 0.0 6 13 152 2 0.0 6 2 153 7 0.0 6 7 154 18 0.0 6 18 155 56 0.0 6 55 1 156 14 0.0 6 14 157 57 0.0 6 57 158 44 0.0 6 44 159 14 0.0 6 14 160 2 0.0 6 2 161 5 0.0 6 5 162 3 0.0 6 3 163 1 0.0 6 1 164 2 0.0 6 2 165 4 0.0 6 4 166 11 0.0 6 11 167 20 0.0 6 20 168 6 0.0 6 6 169 3 0.0 6 3 170 7 0.0 6 7 171 12 0.0 6 12 172 21 0.0 6 21 173 19 0.0 6 19 174 37 0.0 6 37 175 40 0.0 6 40 176 6 0.0 6 6 177 7 0.0 6 7 178 18 0.0 6 18 179 21 0.0 6 21 180 4 0.0 6 4 181 9 0.0 6 9 182 9 0.0 6 9 183 27 0.0 6 27 184 54 0.0 6 54 185 11 0.0 6 11 186 12 0.0 6 12 187 1 0.0 6 1 188 6 0.0 6 6 189 21 0.0 6 21 190 58 0.0 6 58 191 24 0.0 6 24 192 4 0.0 6 4 193 13 0.0 6 13 194 31 0.0 6 30 1 195 71 0.0 6 71 196 2 0.0 6 2 197 3 0.0 6 3 198 4 0.0 6 4 199 6 0.0 6 6 200 13 0.0 6 13 201 3 0.0 6 3 202 1 0.0 6 1 203 18 0.0 6 18 204 29 0.0 6 29 205 2 0.0 6 2 206 5 0.0 6 5 207 20 0.0 6 20 208 4 0.0 6 4 209 3 0.0 6 3 210 6 0.0 6 6 211 28 0.0 6 28 212 6 0.0 6 6 213 3 0.0 6 3 214 3 0.0 6 3 215 4 0.0 6 4 216 2 0.0 6 2 218 8 0.0 6 8 219 14 0.0 6 14 220 15 0.0 6 15 221 21 0.0 6 21 222 4 0.0 6 4 223 9 0.0 6 9 224 9 0.0 6 9 225 18 0.0 6 18 226 2 0.0 6 2 227 1 0.0 6 1 228 1 0.0 6 1 229 3 0.0 6 3 230 15 0.0 6 15 231 1 0.0 6 1 232 4 0.0 6 4 233 4 0.0 6 4 236 5 0.0 6 5 237 3 0.0 6 3 240 4 0.0 6 4 241 5 0.0 6 5 242 1 0.0 6 1 243 2 0.0 6 2 244 2 0.0 6 2 245 1 0.0 6 1 246 1 0.0 6 1 247 2 0.0 6 2 248 8 0.0 6 8 249 3 0.0 6 3 250 3 0.0 6 3 251 2 0.0 6 2 252 2 0.0 6 2 253 3 0.0 6 3 254 10 0.0 6 10 255 5 0.0 6 5 256 4 0.0 6 4 258 3 0.0 6 3 260 2 0.0 6 2 261 3 0.0 6 3 266 1 0.0 6 1 267 1 0.0 6 1 268 1 0.0 6 1 271 3 0.0 6 3 272 1 0.0 6 1 277 1 0.0 6 1 278 3 0.0 6 3 279 1 0.0 6 1 281 1 0.0 6 1 282 1 0.0 6 1 286 1 0.0 6 1 288 1 0.0 6 1 290 6 0.0 6 5 1 291 1 0.0 6 1 293 2 0.0 6 2 295 2 0.0 6 2 297 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 84571 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 340.6 1 1 6 1 21.3 1 1 7 1 5.3 2 1 11 2 0.0 3 2 12 3 0.0 3 2 1 13 12 0.0 3 8 3 1 14 11 0.0 4 10 1 16 17 0.0 4 14 2 0 1 17 13 0.0 5 13 18 12 0.0 5 12 19 10 0.0 5 8 2 20 140 0.0 6 135 5 21 87 0.0 6 86 1 22 49 0.0 6 46 3 23 43 0.0 6 42 1 24 25 0.0 6 23 1 1 25 23 0.0 6 21 2 26 24 0.0 6 23 1 27 29 0.0 6 28 1 28 23 0.0 6 22 1 29 16 0.0 6 15 1 30 20 0.0 6 20 31 18 0.0 6 15 2 1 32 21 0.0 6 20 1 33 23 0.0 6 22 1 34 10 0.0 6 10 35 11 0.0 6 11 36 25 0.0 6 24 1 37 9 0.0 6 9 38 11 0.0 6 10 1 39 15 0.0 6 14 1 40 6 0.0 6 6 41 10 0.0 6 8 2 42 18 0.0 6 18 43 17 0.0 6 12 5 44 17 0.0 6 14 2 0 1 45 19 0.0 6 17 1 0 0 1 46 14 0.0 6 13 0 0 0 1 47 23 0.0 6 23 48 23 0.0 6 21 1 1 49 12 0.0 6 11 1 50 19 0.0 6 17 1 1 51 27 0.0 6 25 2 52 22 0.0 6 21 1 53 27 0.0 6 25 1 0 0 0 1 54 16 0.0 6 14 2 55 20 0.0 6 18 2 56 20 0.0 6 18 2 57 23 0.0 6 22 1 58 22 0.0 6 21 1 59 34 0.0 6 28 5 0 1 60 35 0.0 6 31 4 61 33 0.0 6 26 5 2 62 21 0.0 6 20 1 63 24 0.0 6 19 3 0 1 1 64 41 0.0 6 37 4 65 43 0.0 6 38 5 66 42 0.0 6 39 2 1 67 33 0.0 6 33 68 50 0.0 6 43 7 69 161 0.0 6 152 8 0 0 0 0 1 70 61 0.0 6 57 3 0 0 0 1 71 90 0.0 6 87 3 72 44 0.0 6 43 1 73 54 0.0 6 54 74 58 0.0 6 55 2 1 75 51 0.0 6 43 8 76 46 0.0 6 46 77 39 0.0 6 35 2 0 0 0 1 1 78 50 0.0 6 46 4 79 49 0.0 6 48 1 80 38 0.0 6 35 1 2 81 66 0.0 6 64 2 82 56 0.0 6 53 3 83 55 0.0 6 52 3 84 65 0.0 6 63 2 85 91 0.0 6 85 5 0 0 0 1 86 52 0.0 6 48 2 0 1 0 0 1 87 64 0.0 6 60 3 0 0 0 1 88 54 0.0 6 51 3 89 47 0.0 6 41 6 90 65 0.0 6 62 3 91 50 0.0 6 45 4 0 1 92 71 0.0 6 68 3 93 75 0.0 6 67 8 94 74 0.0 6 68 5 0 0 1 95 95 0.0 6 93 2 96 78 0.0 6 74 4 97 102 0.0 6 101 1 98 85 0.0 6 82 1 1 1 99 98 0.0 6 96 2 100 93 0.0 6 90 2 0 0 1 101 85 0.0 6 82 3 102 131 0.0 6 127 4 103 99 0.0 6 94 5 104 125 0.0 6 124 1 105 158 0.0 6 154 3 0 0 0 0 1 106 208 0.0 6 204 4 107 137 0.0 6 131 6 108 110 0.0 6 102 7 0 0 0 0 1 109 132 0.0 6 131 1 110 115 0.0 6 109 6 111 138 0.0 6 137 1 112 159 0.0 6 152 5 0 0 2 113 172 0.0 6 167 4 0 0 0 1 114 180 0.0 6 174 5 0 0 1 115 179 0.0 6 167 12 116 216 0.0 6 209 3 0 1 2 1 117 319 0.0 6 308 5 1 1 3 1 118 406 0.0 6 386 16 1 2 1 119 1778 0.0 6 1723 38 0 1 16 120 485 0.0 6 471 9 0 1 4 121 204 0.0 6 201 3 122 185 0.0 6 179 5 0 1 123 234 0.0 6 226 5 2 0 0 0 1 124 221 0.0 6 213 8 125 254 0.0 6 250 4 126 283 0.0 6 275 7 0 0 1 127 341 0.0 6 333 7 1 128 252 0.0 6 248 4 129 348 0.0 6 337 9 0 0 0 0 2 130 392 0.0 6 382 9 0 0 0 0 1 131 350 0.0 6 345 4 0 0 0 1 132 366 0.0 6 349 14 0 1 1 0 1 133 406 0.0 6 390 15 0 0 0 0 1 134 484 0.0 6 474 10 135 460 0.0 6 451 9 136 577 0.0 6 561 8 2 1 0 2 3 137 662 0.0 6 641 18 0 1 0 0 2 138 627 0.0 6 616 10 1 139 974 0.0 6 965 6 0 0 0 0 3 140 1474 0.0 6 1467 6 0 0 0 1 141 988 0.0 6 975 10 0 0 0 0 3 142 1068 0.0 6 1050 17 0 0 0 0 1 143 1122 0.0 6 1111 10 0 0 1 144 1505 0.0 6 1494 8 0 1 1 0 1 145 1096 0.0 6 1085 10 0 0 0 1 146 1082 0.0 6 1064 16 1 0 0 0 1 147 1334 0.0 6 1320 11 1 0 1 1 148 1236 0.0 6 1212 24 149 1402 0.0 6 1381 20 1 150 1147 0.0 6 1132 14 1 151 1297 0.0 6 1277 18 0 0 1 1 152 1657 0.0 6 1620 31 1 1 0 1 3 153 1446 0.0 6 1428 17 0 0 0 0 1 154 1604 0.0 6 1583 19 0 0 0 2 155 1551 0.0 6 1526 17 3 0 2 0 3 156 1546 0.0 6 1533 12 0 1 157 1467 0.0 6 1447 18 2 158 1191 0.0 6 1173 15 1 0 0 1 1 159 1428 0.0 6 1410 16 1 0 0 0 1 160 1363 0.0 6 1348 13 2 161 1205 0.0 6 1186 13 3 1 2 162 1287 0.0 6 1272 13 1 0 0 1 163 1152 0.0 6 1138 12 0 0 0 1 1 164 1040 0.0 6 1019 20 0 0 0 0 1 165 1042 0.0 6 1034 8 166 998 0.0 6 987 11 167 1322 0.0 6 1312 9 0 0 1 168 1219 0.0 6 1205 11 1 0 2 169 967 0.0 6 955 11 0 1 170 1130 0.0 6 1115 13 0 0 2 171 1228 0.0 6 1210 15 0 2 1 172 1195 0.0 6 1190 5 173 1054 0.0 6 1048 5 0 0 0 1 174 1163 0.0 6 1158 5 175 1337 0.0 6 1328 7 0 1 0 0 1 176 1066 0.0 6 1058 6 1 0 0 0 1 177 1053 0.0 6 1044 8 1 178 1077 0.0 6 1067 10 179 895 0.0 6 890 5 180 886 0.0 6 876 8 0 1 0 0 1 181 702 0.0 6 696 5 1 182 656 0.0 6 651 5 183 762 0.0 6 752 8 0 0 1 1 184 813 0.0 6 807 3 1 1 1 185 967 0.0 6 957 8 1 1 186 986 0.0 6 977 6 0 1 1 1 187 959 0.0 6 951 8 188 698 0.0 6 696 2 189 643 0.0 6 639 3 1 190 624 0.0 6 618 5 1 191 706 0.0 6 704 2 192 593 0.0 6 588 5 193 644 0.0 6 636 8 194 395 0.0 6 393 2 195 443 0.0 6 439 4 196 327 0.0 6 322 5 197 302 0.0 6 298 3 0 0 0 1 198 514 0.0 6 511 3 199 407 0.0 6 404 3 200 378 0.0 6 377 1 201 355 0.0 6 354 1 202 336 0.0 6 334 0 0 1 0 1 203 280 0.0 6 274 5 0 1 204 280 0.0 6 279 1 205 239 0.0 6 238 1 206 194 0.0 6 192 2 207 194 0.0 6 193 0 0 0 0 0 1 208 202 0.0 6 199 2 1 209 153 0.0 6 152 1 210 118 0.0 6 116 1 0 0 1 211 121 0.0 6 120 1 212 125 0.0 6 125 213 110 0.0 6 109 1 214 124 0.0 6 123 1 215 81 0.0 6 79 2 216 120 0.0 6 119 1 217 97 0.0 6 97 218 129 0.0 6 126 3 219 111 0.0 6 105 5 0 0 0 1 220 78 0.0 6 77 1 221 66 0.0 6 66 222 52 0.0 6 51 1 223 101 0.0 6 98 3 224 227 0.0 6 215 11 1 225 120 0.0 6 118 1 1 226 82 0.0 6 79 3 227 261 0.0 6 252 9 228 50 0.0 6 50 229 91 0.0 6 86 5 230 21 0.0 6 21 231 99 0.0 6 96 2 0 0 1 232 63 0.0 6 61 2 233 84 0.0 6 76 8 234 76 0.0 6 76 235 16 0.0 6 15 1 236 13 0.0 6 13 237 6 0.0 6 6 238 9 0.0 6 9 239 1 0.0 6 1 240 6 0.0 6 6 241 18 0.0 6 18 242 8 0.0 6 7 0 1 243 31 0.0 6 31 244 42 0.0 6 42 245 11 0.0 6 11 246 18 0.0 6 17 1 247 11 0.0 6 10 1 248 8 0.0 6 8 249 22 0.0 6 22 250 7 0.0 6 6 1 251 19 0.0 6 19 252 16 0.0 6 15 1 253 7 0.0 6 7 254 14 0.0 6 14 255 7 0.0 6 7 256 1 0.0 6 1 257 1 0.0 6 1 279 2 0.0 6 2 280 11 0.0 6 11 281 42 0.0 6 42 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2582 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 5 0.0 6 5 70 23 0.0 6 23 71 81 0.0 6 81 72 15 0.0 6 15 73 31 0.0 6 31 74 18 0.0 6 18 75 55 0.0 6 55 76 28 0.0 6 28 77 27 0.0 6 27 78 26 0.0 6 26 79 18 0.0 6 18 80 52 0.0 6 51 0 0 1 81 5 0.0 6 5 82 21 0.0 6 21 83 10 0.0 6 10 84 15 0.0 6 15 85 8 0.0 6 8 86 6 0.0 6 6 87 41 0.0 6 41 88 34 0.0 6 34 89 14 0.0 6 14 90 10 0.0 6 10 91 19 0.0 6 19 92 32 0.0 6 31 0 0 0 1 93 22 0.0 6 22 94 31 0.0 6 30 1 95 1 0.0 6 1 96 11 0.0 6 11 97 12 0.0 6 12 98 53 0.0 6 53 99 22 0.0 6 21 1 100 14 0.0 6 13 0 0 1 101 17 0.0 6 16 1 102 51 0.0 6 50 0 0 0 1 103 14 0.0 6 14 104 15 0.0 6 15 105 15 0.0 6 15 106 4 0.0 6 4 107 30 0.0 6 29 0 0 0 1 108 51 0.0 6 51 109 25 0.0 6 25 110 13 0.0 6 13 111 33 0.0 6 33 112 17 0.0 6 17 113 8 0.0 6 8 114 13 0.0 6 13 115 20 0.0 6 20 116 3 0.0 6 3 117 3 0.0 6 3 118 16 0.0 6 15 0 0 0 1 119 102 0.0 6 102 120 4 0.0 6 4 121 4 0.0 6 4 122 8 0.0 6 8 123 15 0.0 6 15 124 4 0.0 6 4 125 21 0.0 6 21 126 13 0.0 6 13 127 18 0.0 6 18 128 13 0.0 6 13 129 44 0.0 6 44 130 10 0.0 6 10 131 14 0.0 6 14 132 60 0.0 6 60 133 59 0.0 6 59 134 7 0.0 6 7 135 14 0.0 6 13 0 0 1 136 27 0.0 6 27 137 18 0.0 6 17 0 0 0 1 138 44 0.0 6 44 139 24 0.0 6 24 140 20 0.0 6 20 141 5 0.0 6 5 142 3 0.0 6 2 0 0 0 1 143 24 0.0 6 24 144 12 0.0 6 12 145 20 0.0 6 20 148 14 0.0 6 14 149 18 0.0 6 18 150 27 0.0 6 27 151 2 0.0 6 2 152 11 0.0 6 11 153 19 0.0 6 18 0 0 1 154 61 0.0 6 61 155 15 0.0 6 15 156 50 0.0 6 50 157 64 0.0 6 64 158 6 0.0 6 6 159 1 0.0 6 1 160 4 0.0 6 4 161 2 0.0 6 2 162 7 0.0 6 6 0 0 0 1 163 7 0.0 6 7 164 10 0.0 6 10 165 9 0.0 6 9 166 16 0.0 6 16 167 2 0.0 6 2 168 12 0.0 6 11 0 0 0 1 169 6 0.0 6 6 170 9 0.0 6 8 1 171 14 0.0 6 14 172 22 0.0 6 22 173 51 0.0 6 51 174 34 0.0 6 34 175 7 0.0 6 6 0 0 1 177 11 0.0 6 11 178 27 0.0 6 27 179 2 0.0 6 2 180 8 0.0 6 7 1 181 7 0.0 6 7 182 20 0.0 6 20 183 64 0.0 6 64 184 3 0.0 6 1 2 185 4 0.0 6 4 186 5 0.0 6 4 0 0 0 1 188 17 0.0 6 17 189 37 0.0 6 37 190 22 0.0 6 22 191 4 0.0 6 4 192 7 0.0 6 6 0 0 1 193 9 0.0 6 9 194 37 0.0 6 37 195 1 0.0 6 1 196 2 0.0 6 2 197 1 0.0 6 1 198 2 0.0 6 2 199 7 0.0 6 7 200 1 0.0 6 1 202 2 0.0 6 2 203 21 0.0 6 21 206 3 0.0 6 3 207 1 0.0 6 1 209 1 0.0 6 1 210 6 0.0 6 6 211 2 0.0 6 2 212 5 0.0 6 5 213 1 0.0 6 1 215 1 0.0 6 1 218 1 0.0 6 1 219 3 0.0 6 3 220 7 0.0 6 7 221 2 0.0 6 2 222 2 0.0 6 2 224 4 0.0 6 4 227 1 0.0 6 1 228 3 0.0 6 3 229 2 0.0 6 2 243 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_20_107_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_20_334_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_20_107_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_20_334_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.86 s (82 µs/read; 0.73 M reads/minute). === Summary === Total read pairs processed: 59,492 Read 1 with adapter: 59,486 (100.0%) Read 2 with adapter: 59,462 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 36 (0.1%) Pairs written (passing filters): 59,456 (99.9%) Total basepairs processed: 30,673,377 bp Read 1: 17,258,943 bp Read 2: 13,414,434 bp Total written (filtered): 3,745,030 bp (12.2%) Read 1: 1,842,895 bp Read 2: 1,902,135 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 59483 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 232.4 1 0 1 6 2 14.5 1 0 0 2 7 1 3.6 2 1 9 1 0.2 2 0 1 11 1 0.0 3 1 15 1 0.0 4 1 17 1 0.0 5 1 20 7 0.0 6 7 21 11 0.0 6 11 22 2 0.0 6 2 23 4 0.0 6 4 24 1 0.0 6 0 0 1 25 10 0.0 6 10 26 12 0.0 6 10 2 27 2 0.0 6 2 28 4 0.0 6 4 29 1 0.0 6 1 30 1 0.0 6 1 31 1 0.0 6 1 32 1 0.0 6 1 33 4 0.0 6 4 34 2 0.0 6 2 35 1 0.0 6 1 38 4 0.0 6 3 1 39 2 0.0 6 2 40 3 0.0 6 3 41 4 0.0 6 4 43 1 0.0 6 0 1 44 3 0.0 6 3 45 5 0.0 6 5 46 3 0.0 6 3 47 1 0.0 6 1 51 1 0.0 6 1 53 5 0.0 6 4 1 54 1 0.0 6 1 56 5 0.0 6 5 57 2 0.0 6 2 58 1 0.0 6 1 60 3 0.0 6 3 61 1 0.0 6 1 62 2 0.0 6 1 1 63 2 0.0 6 2 64 2 0.0 6 2 65 2 0.0 6 2 68 2 0.0 6 2 69 2 0.0 6 2 70 1 0.0 6 1 72 1 0.0 6 1 73 2 0.0 6 2 77 3 0.0 6 3 78 2 0.0 6 1 1 80 1 0.0 6 1 82 1 0.0 6 1 86 9 0.0 6 9 87 3 0.0 6 2 1 89 5 0.0 6 5 90 2 0.0 6 1 1 91 2 0.0 6 2 92 2 0.0 6 2 93 1 0.0 6 1 94 1 0.0 6 1 97 4 0.0 6 4 98 5 0.0 6 5 99 1 0.0 6 1 100 6 0.0 6 6 101 10 0.0 6 10 102 6 0.0 6 6 103 6 0.0 6 5 1 104 4 0.0 6 4 105 1 0.0 6 1 106 3 0.0 6 3 107 6 0.0 6 5 0 1 108 12 0.0 6 11 1 109 3 0.0 6 2 1 111 3 0.0 6 3 112 6 0.0 6 5 0 1 113 3 0.0 6 3 114 2 0.0 6 2 115 7 0.0 6 5 2 116 3 0.0 6 3 117 7 0.0 6 7 118 8 0.0 6 5 3 119 2 0.0 6 2 120 2 0.0 6 2 121 12 0.0 6 10 1 1 122 17 0.0 6 13 4 123 10 0.0 6 10 124 10 0.0 6 9 1 125 18 0.0 6 17 1 126 6 0.0 6 6 127 8 0.0 6 7 0 1 128 22 0.0 6 21 1 129 9 0.0 6 8 1 130 7 0.0 6 7 131 3 0.0 6 3 132 23 0.0 6 22 1 133 4 0.0 6 4 134 21 0.0 6 21 135 19 0.0 6 19 136 20 0.0 6 19 1 137 27 0.0 6 27 138 12 0.0 6 12 139 12 0.0 6 9 3 140 17 0.0 6 17 141 12 0.0 6 11 1 142 13 0.0 6 13 143 28 0.0 6 27 1 144 23 0.0 6 23 145 17 0.0 6 17 146 4 0.0 6 4 147 6 0.0 6 6 148 17 0.0 6 17 149 25 0.0 6 24 1 150 9 0.0 6 8 1 151 21 0.0 6 21 152 31 0.0 6 31 153 21 0.0 6 20 1 154 18 0.0 6 18 155 16 0.0 6 16 156 14 0.0 6 14 157 8 0.0 6 7 1 158 6 0.0 6 5 0 0 0 1 159 7 0.0 6 7 160 12 0.0 6 12 161 17 0.0 6 16 1 162 15 0.0 6 14 1 163 13 0.0 6 12 1 164 29 0.0 6 28 1 165 20 0.0 6 20 166 7 0.0 6 7 167 14 0.0 6 14 168 28 0.0 6 27 1 169 16 0.0 6 15 1 170 42 0.0 6 40 2 171 34 0.0 6 32 2 172 18 0.0 6 18 173 20 0.0 6 20 174 30 0.0 6 30 175 14 0.0 6 14 176 26 0.0 6 25 1 177 36 0.0 6 33 3 178 17 0.0 6 17 179 16 0.0 6 12 4 180 28 0.0 6 28 181 18 0.0 6 17 1 182 23 0.0 6 21 2 183 59 0.0 6 58 1 184 28 0.0 6 27 1 185 42 0.0 6 40 2 186 53 0.0 6 49 4 187 39 0.0 6 37 2 188 35 0.0 6 34 1 189 19 0.0 6 17 2 190 25 0.0 6 24 1 191 47 0.0 6 46 1 192 58 0.0 6 56 2 193 64 0.0 6 64 194 62 0.0 6 59 3 195 73 0.0 6 70 3 196 57 0.0 6 55 2 197 82 0.0 6 79 3 198 74 0.0 6 70 4 199 83 0.0 6 80 3 200 59 0.0 6 58 1 201 58 0.0 6 56 2 202 53 0.0 6 53 203 89 0.0 6 85 4 204 99 0.0 6 96 1 2 205 139 0.0 6 138 1 206 91 0.0 6 91 207 141 0.0 6 136 5 208 189 0.0 6 184 5 209 215 0.0 6 210 5 210 156 0.0 6 150 5 1 211 372 0.0 6 363 9 212 246 0.0 6 235 10 1 213 209 0.0 6 199 10 214 359 0.0 6 352 7 215 114 0.0 6 108 5 0 1 216 397 0.0 6 389 8 217 166 0.0 6 156 10 218 185 0.0 6 182 3 219 106 0.0 6 102 4 220 138 0.0 6 138 221 69 0.0 6 65 4 222 203 0.0 6 198 5 223 97 0.0 6 90 7 224 270 0.0 6 262 7 1 225 183 0.0 6 178 5 226 501 0.0 6 488 11 2 227 341 0.0 6 336 5 228 297 0.0 6 291 6 229 368 0.0 6 364 4 230 319 0.0 6 311 7 0 1 231 295 0.0 6 287 8 232 235 0.0 6 232 3 233 200 0.0 6 194 5 0 1 234 218 0.0 6 213 5 235 129 0.0 6 126 3 236 157 0.0 6 153 4 237 185 0.0 6 181 4 238 178 0.0 6 175 3 239 268 0.0 6 261 7 240 740 0.0 6 731 9 241 191 0.0 6 186 5 242 247 0.0 6 240 7 243 163 0.0 6 158 5 244 158 0.0 6 153 5 245 185 0.0 6 180 5 246 242 0.0 6 234 8 247 515 0.0 6 509 6 248 248 0.0 6 243 4 1 249 236 0.0 6 231 5 250 677 0.0 6 668 8 1 251 336 0.0 6 325 11 252 1357 0.0 6 1334 23 253 393 0.0 6 388 5 254 325 0.0 6 315 9 1 255 563 0.0 6 551 11 1 256 544 0.0 6 518 22 3 1 257 900 0.0 6 877 22 1 258 879 0.0 6 855 23 1 259 1696 0.0 6 1669 27 260 783 0.0 6 767 15 0 1 261 1653 0.0 6 1625 26 2 262 704 0.0 6 686 17 1 263 1799 0.0 6 1771 28 264 518 0.0 6 511 6 1 265 439 0.0 6 432 7 266 701 0.0 6 687 14 267 891 0.0 6 879 12 268 476 0.0 6 461 14 1 269 957 0.0 6 938 18 1 270 693 0.0 6 673 20 271 730 0.0 6 716 13 1 272 463 0.0 6 453 10 273 485 0.0 6 478 6 0 1 274 448 0.0 6 441 7 275 816 0.0 6 778 37 1 276 2342 0.0 6 2300 42 277 1918 0.0 6 1862 52 3 1 278 2199 0.0 6 2138 61 279 15558 0.0 6 15267 282 7 1 0 0 1 280 3912 0.0 6 3769 140 2 1 281 56 0.0 6 50 5 1 282 3 0.0 6 2 0 1 283 4 0.0 6 3 0 0 1 284 2 0.0 6 2 285 2 0.0 6 2 287 1 0.0 6 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 3 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 76 1 0.0 6 1 116 1 0.0 6 1 124 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 59460 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 58.1 1 1 10 5 0.1 3 5 11 3 0.0 3 3 13 7 0.0 3 5 2 14 4 0.0 4 3 1 15 1 0.0 4 0 1 16 8 0.0 4 7 1 17 9 0.0 5 8 1 18 7 0.0 5 6 1 19 9 0.0 5 8 1 20 23 0.0 6 22 1 21 39 0.0 6 39 22 20 0.0 6 20 23 14 0.0 6 13 0 0 1 24 21 0.0 6 20 1 25 13 0.0 6 12 1 26 7 0.0 6 6 1 27 12 0.0 6 12 28 6 0.0 6 5 1 29 13 0.0 6 11 2 30 3 0.0 6 3 31 5 0.0 6 4 1 32 3 0.0 6 3 33 3 0.0 6 3 34 10 0.0 6 8 1 1 35 8 0.0 6 8 36 5 0.0 6 5 37 6 0.0 6 5 0 0 0 1 38 6 0.0 6 5 0 1 39 8 0.0 6 6 2 40 8 0.0 6 8 41 3 0.0 6 2 0 0 0 1 42 4 0.0 6 3 0 0 0 1 43 9 0.0 6 9 44 17 0.0 6 16 1 45 1 0.0 6 0 1 46 14 0.0 6 12 1 1 47 16 0.0 6 16 48 10 0.0 6 8 1 1 49 12 0.0 6 11 1 50 13 0.0 6 13 51 4 0.0 6 4 52 23 0.0 6 22 1 53 8 0.0 6 8 54 20 0.0 6 19 1 55 7 0.0 6 6 1 56 15 0.0 6 11 3 0 0 1 57 16 0.0 6 13 3 58 14 0.0 6 14 59 4 0.0 6 4 60 15 0.0 6 11 3 0 0 1 61 12 0.0 6 10 2 62 6 0.0 6 6 63 12 0.0 6 9 3 64 17 0.0 6 16 1 65 1 0.0 6 1 66 24 0.0 6 19 3 1 1 67 135 0.0 6 127 7 1 68 23 0.0 6 21 2 69 44 0.0 6 41 2 0 0 1 70 14 0.0 6 13 1 71 25 0.0 6 23 2 72 20 0.0 6 17 3 73 8 0.0 6 8 74 7 0.0 6 7 75 8 0.0 6 7 1 76 13 0.0 6 13 77 6 0.0 6 6 78 15 0.0 6 13 2 79 15 0.0 6 15 80 7 0.0 6 6 0 1 81 4 0.0 6 4 82 23 0.0 6 16 4 2 0 1 83 49 0.0 6 49 84 16 0.0 6 16 85 20 0.0 6 16 3 1 86 19 0.0 6 15 3 0 0 1 87 43 0.0 6 39 3 0 0 1 88 12 0.0 6 11 1 89 20 0.0 6 17 3 90 26 0.0 6 23 3 91 14 0.0 6 11 3 92 16 0.0 6 16 93 42 0.0 6 37 4 1 94 33 0.0 6 29 4 95 40 0.0 6 38 2 96 39 0.0 6 35 2 1 0 1 97 16 0.0 6 15 1 98 24 0.0 6 23 1 99 24 0.0 6 22 1 0 1 100 19 0.0 6 17 2 101 21 0.0 6 19 2 102 62 0.0 6 56 4 0 0 1 1 103 54 0.0 6 48 5 0 0 1 104 42 0.0 6 41 1 105 51 0.0 6 47 3 1 106 31 0.0 6 30 1 107 39 0.0 6 37 2 108 22 0.0 6 21 1 109 27 0.0 6 27 110 36 0.0 6 35 1 111 38 0.0 6 31 7 112 24 0.0 6 24 113 23 0.0 6 21 2 114 41 0.0 6 38 3 115 42 0.0 6 38 3 1 116 39 0.0 6 38 1 117 27 0.0 6 21 2 1 1 1 1 118 32 0.0 6 30 2 119 33 0.0 6 33 120 72 0.0 6 67 5 121 34 0.0 6 32 2 122 39 0.0 6 37 2 123 40 0.0 6 36 3 0 1 124 53 0.0 6 49 4 125 49 0.0 6 47 2 126 47 0.0 6 43 4 127 55 0.0 6 50 4 1 128 44 0.0 6 39 5 129 29 0.0 6 25 4 130 35 0.0 6 31 4 131 84 0.0 6 82 2 132 116 0.0 6 111 5 133 69 0.0 6 68 1 134 127 0.0 6 119 7 1 135 193 0.0 6 186 7 136 63 0.0 6 59 3 1 137 100 0.0 6 99 1 138 42 0.0 6 39 3 139 91 0.0 6 87 4 140 167 0.0 6 162 5 141 141 0.0 6 135 6 142 193 0.0 6 188 4 1 143 187 0.0 6 181 5 1 144 277 0.0 6 267 8 1 0 1 145 133 0.0 6 130 3 146 189 0.0 6 181 6 1 0 0 0 1 147 107 0.0 6 98 9 148 129 0.0 6 127 2 149 240 0.0 6 230 9 0 0 1 150 475 0.0 6 461 14 151 161 0.0 6 159 2 152 354 0.0 6 338 14 1 0 0 1 153 268 0.0 6 258 10 154 215 0.0 6 207 8 155 165 0.0 6 158 7 156 198 0.0 6 191 6 0 0 1 157 321 0.0 6 311 10 158 337 0.0 6 323 14 159 152 0.0 6 143 9 160 270 0.0 6 261 9 161 156 0.0 6 151 5 162 131 0.0 6 126 5 163 280 0.0 6 271 7 2 164 264 0.0 6 253 10 1 165 327 0.0 6 320 7 166 436 0.0 6 428 8 167 315 0.0 6 298 14 1 1 1 168 552 0.0 6 528 24 169 210 0.0 6 206 3 0 1 170 299 0.0 6 287 11 1 171 583 0.0 6 567 15 1 172 262 0.0 6 252 10 173 314 0.0 6 304 10 174 484 0.0 6 471 12 0 0 1 175 500 0.0 6 490 9 0 0 1 176 453 0.0 6 435 14 2 0 2 177 522 0.0 6 501 20 1 178 391 0.0 6 380 10 1 179 477 0.0 6 463 12 2 180 551 0.0 6 541 10 181 580 0.0 6 561 18 1 182 165 0.0 6 153 11 0 1 183 577 0.0 6 561 15 1 184 531 0.0 6 515 15 0 0 1 185 503 0.0 6 487 15 1 186 928 0.0 6 902 26 187 783 0.0 6 753 26 3 0 1 188 891 0.0 6 857 32 1 0 1 189 947 0.0 6 918 26 2 0 1 190 1186 0.0 6 1150 34 1 0 1 191 1367 0.0 6 1339 28 192 591 0.0 6 570 19 1 1 193 989 0.0 6 966 21 1 1 194 904 0.0 6 878 26 195 888 0.0 6 853 34 0 1 196 1611 0.0 6 1564 42 3 2 197 518 0.0 6 498 19 0 0 1 198 1472 0.0 6 1432 36 1 0 3 199 1046 0.0 6 1025 20 0 0 0 1 200 971 0.0 6 948 21 1 0 1 201 1060 0.0 6 1036 23 1 202 1028 0.0 6 1004 24 203 656 0.0 6 638 16 1 1 204 936 0.0 6 916 20 205 762 0.0 6 740 21 0 1 206 649 0.0 6 631 17 0 0 1 207 878 0.0 6 849 28 0 0 0 0 1 208 1083 0.0 6 1065 18 209 144 0.0 6 139 4 0 1 210 319 0.0 6 306 12 0 1 211 794 0.0 6 775 19 212 517 0.0 6 510 5 2 213 295 0.0 6 288 7 214 742 0.0 6 719 21 1 0 1 215 851 0.0 6 831 20 216 1040 0.0 6 1021 16 2 0 1 217 574 0.0 6 562 12 218 347 0.0 6 342 5 219 263 0.0 6 257 6 220 132 0.0 6 124 8 221 326 0.0 6 315 11 222 448 0.0 6 443 4 0 0 1 223 283 0.0 6 280 3 224 215 0.0 6 211 3 0 1 225 278 0.0 6 270 7 0 1 226 436 0.0 6 413 21 1 0 1 227 874 0.0 6 854 20 228 888 0.0 6 864 16 3 0 4 1 229 1084 0.0 6 1054 29 1 230 2497 0.0 6 2425 68 2 0 2 231 630 0.0 6 602 26 1 0 1 232 1301 0.0 6 1281 19 0 0 1 233 155 0.0 6 151 4 234 345 0.0 6 335 9 0 1 235 208 0.0 6 201 7 236 179 0.0 6 175 4 237 176 0.0 6 170 6 238 827 0.0 6 815 11 0 0 1 239 99 0.0 6 96 3 240 96 0.0 6 94 2 241 220 0.0 6 217 3 242 143 0.0 6 139 4 243 52 0.0 6 51 1 244 70 0.0 6 66 4 245 322 0.0 6 312 9 0 0 1 246 180 0.0 6 173 7 247 163 0.0 6 160 3 248 128 0.0 6 127 0 0 0 1 249 71 0.0 6 69 2 250 64 0.0 6 62 2 251 258 0.0 6 254 4 252 33 0.0 6 30 2 1 253 22 0.0 6 20 2 254 23 0.0 6 22 1 255 10 0.0 6 10 256 6 0.0 6 5 1 257 2 0.0 6 2 258 1 0.0 6 1 259 1 0.0 6 1 263 1 0.0 6 1 265 1 0.0 6 1 266 1 0.0 6 1 272 1 0.0 6 1 277 4 0.0 6 4 278 45 0.0 6 44 1 279 97 0.0 6 93 3 0 0 0 0 1 280 3 0.0 6 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 85 1 0.0 6 1 110 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_01_96_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_01_323_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_01_96_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_01_323_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_02_97_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_02_324_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_02_97_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_02_324_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_03_98_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_03_325_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_03_98_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_03_325_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_04_99_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_04_326_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_04_99_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_04_326_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_05_100_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_05_327_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_05_100_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_05_327_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_06_101_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_06_328_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_06_101_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_06_328_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_15_102_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_15_329_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_15_102_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_15_329_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_16_103_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_16_330_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_16_103_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_16_330_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_17_104_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_17_331_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_17_104_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_17_331_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_18_105_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_18_332_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_18_105_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_18_332_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_19_106_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_19_333_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_19_106_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_19_333_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_20_107_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_09-Rev_20_334_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_20_107_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_09-Rev_20_334_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_01_108_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_01_335_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_01_108_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_01_335_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.62 s (70 µs/read; 0.86 M reads/minute). === Summary === Total read pairs processed: 80,582 Read 1 with adapter: 80,569 (100.0%) Read 2 with adapter: 80,534 (99.9%) == Read fate breakdown == Pairs that were too short: 4 (0.0%) Pairs discarded as untrimmed: 61 (0.1%) Pairs written (passing filters): 80,517 (99.9%) Total basepairs processed: 39,568,492 bp Read 1: 22,480,823 bp Read 2: 17,087,669 bp Total written (filtered): 5,001,604 bp (12.6%) Read 1: 2,583,177 bp Read 2: 2,418,427 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 80403 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1259.1 0 0 2 4 2 314.8 1 2 6 1 19.7 1 0 0 1 7 1 4.9 2 0 1 16 2 0.0 4 1 1 17 1 0.0 5 1 20 14 0.0 6 12 2 21 14 0.0 6 14 22 10 0.0 6 8 2 23 8 0.0 6 8 24 8 0.0 6 8 25 12 0.0 6 11 1 26 6 0.0 6 6 27 8 0.0 6 6 1 0 1 28 2 0.0 6 1 1 29 3 0.0 6 3 30 2 0.0 6 2 31 2 0.0 6 2 32 1 0.0 6 0 1 33 4 0.0 6 4 34 2 0.0 6 2 35 4 0.0 6 3 1 36 3 0.0 6 3 37 3 0.0 6 3 38 8 0.0 6 5 1 1 0 1 39 2 0.0 6 2 40 6 0.0 6 6 41 2 0.0 6 2 42 7 0.0 6 7 43 2 0.0 6 2 44 4 0.0 6 3 1 45 9 0.0 6 8 1 46 4 0.0 6 2 2 47 4 0.0 6 4 49 2 0.0 6 2 50 9 0.0 6 9 51 1 0.0 6 0 0 1 52 5 0.0 6 5 53 2 0.0 6 2 55 1 0.0 6 1 57 2 0.0 6 1 0 1 59 2 0.0 6 2 60 5 0.0 6 5 61 3 0.0 6 2 1 62 2 0.0 6 2 63 1 0.0 6 1 64 9 0.0 6 6 3 65 4 0.0 6 4 66 1 0.0 6 0 1 67 4 0.0 6 4 68 7 0.0 6 6 1 69 2 0.0 6 2 70 2 0.0 6 2 71 1 0.0 6 0 1 72 2 0.0 6 2 73 3 0.0 6 3 74 3 0.0 6 3 75 1 0.0 6 1 76 1 0.0 6 1 77 3 0.0 6 2 1 78 4 0.0 6 4 79 3 0.0 6 3 81 3 0.0 6 3 82 1 0.0 6 1 83 3 0.0 6 3 84 1 0.0 6 1 85 3 0.0 6 1 1 1 86 18 0.0 6 17 1 87 13 0.0 6 13 88 2 0.0 6 2 89 12 0.0 6 12 90 6 0.0 6 5 1 91 2 0.0 6 2 92 6 0.0 6 4 2 93 5 0.0 6 5 94 5 0.0 6 5 95 3 0.0 6 2 1 96 1 0.0 6 1 97 12 0.0 6 12 98 10 0.0 6 9 1 99 6 0.0 6 5 1 100 11 0.0 6 10 1 101 16 0.0 6 12 3 1 102 15 0.0 6 14 1 103 6 0.0 6 4 2 104 5 0.0 6 5 105 17 0.0 6 17 106 7 0.0 6 5 2 107 16 0.0 6 14 2 108 19 0.0 6 19 109 9 0.0 6 8 1 110 6 0.0 6 6 111 10 0.0 6 9 1 112 19 0.0 6 19 113 11 0.0 6 10 1 114 6 0.0 6 5 1 115 13 0.0 6 13 116 8 0.0 6 8 117 13 0.0 6 12 1 118 16 0.0 6 14 2 119 16 0.0 6 15 1 120 5 0.0 6 5 121 8 0.0 6 8 122 20 0.0 6 18 1 1 123 16 0.0 6 16 124 15 0.0 6 14 1 125 9 0.0 6 9 126 9 0.0 6 8 1 127 15 0.0 6 11 4 128 21 0.0 6 19 2 129 13 0.0 6 12 1 130 7 0.0 6 7 131 7 0.0 6 7 132 15 0.0 6 15 133 28 0.0 6 25 3 134 36 0.0 6 33 3 135 38 0.0 6 37 1 136 19 0.0 6 19 137 30 0.0 6 30 138 25 0.0 6 25 139 23 0.0 6 22 1 140 52 0.0 6 48 4 141 89 0.0 6 89 142 130 0.0 6 129 1 143 130 0.0 6 128 2 144 92 0.0 6 91 1 145 71 0.0 6 70 1 146 125 0.0 6 124 1 147 92 0.0 6 91 1 148 73 0.0 6 73 149 67 0.0 6 65 2 150 84 0.0 6 82 2 151 70 0.0 6 67 3 152 65 0.0 6 63 2 153 47 0.0 6 46 1 154 71 0.0 6 70 1 155 68 0.0 6 67 0 1 156 65 0.0 6 63 2 157 70 0.0 6 69 1 158 58 0.0 6 56 2 159 50 0.0 6 48 2 160 64 0.0 6 63 1 161 86 0.0 6 85 1 162 66 0.0 6 66 163 58 0.0 6 57 1 164 79 0.0 6 79 165 63 0.0 6 62 1 166 47 0.0 6 46 1 167 52 0.0 6 50 2 168 71 0.0 6 71 169 70 0.0 6 67 3 170 107 0.0 6 103 4 171 77 0.0 6 75 2 172 167 0.0 6 161 6 173 96 0.0 6 93 2 1 174 105 0.0 6 102 3 175 75 0.0 6 75 176 95 0.0 6 92 3 177 59 0.0 6 58 1 178 62 0.0 6 62 179 159 0.0 6 154 4 1 180 110 0.0 6 108 2 181 171 0.0 6 166 5 182 188 0.0 6 185 3 183 98 0.0 6 96 2 184 80 0.0 6 74 5 1 185 172 0.0 6 167 5 186 244 0.0 6 237 7 187 146 0.0 6 142 4 188 235 0.0 6 230 4 1 189 141 0.0 6 136 5 190 245 0.0 6 235 9 1 191 228 0.0 6 225 3 192 209 0.0 6 198 11 193 336 0.0 6 326 10 194 272 0.0 6 262 9 0 1 195 391 0.0 6 385 6 196 452 0.0 6 440 11 1 197 502 0.0 6 492 8 1 1 198 554 0.0 6 535 19 199 357 0.0 6 341 13 2 0 1 200 531 0.0 6 510 21 201 385 0.0 6 376 9 202 435 0.0 6 428 7 203 354 0.0 6 342 12 204 421 0.0 6 412 8 0 0 0 1 205 509 0.0 6 496 12 0 0 0 1 206 633 0.0 6 619 14 207 404 0.0 6 396 8 208 332 0.0 6 323 9 209 255 0.0 6 250 5 210 292 0.0 6 283 9 211 313 0.0 6 302 10 1 212 355 0.0 6 348 6 1 213 415 0.0 6 404 8 2 0 0 0 1 214 532 0.0 6 522 10 215 456 0.0 6 448 7 1 216 303 0.0 6 293 9 1 217 328 0.0 6 323 5 218 404 0.0 6 395 9 219 344 0.0 6 334 9 0 1 220 255 0.0 6 243 11 1 221 258 0.0 6 250 7 1 222 603 0.0 6 588 15 223 770 0.0 6 749 17 3 1 224 574 0.0 6 562 12 225 663 0.0 6 656 7 226 556 0.0 6 543 13 227 415 0.0 6 411 4 228 363 0.0 6 352 11 229 303 0.0 6 293 10 230 472 0.0 6 460 12 231 346 0.0 6 342 4 232 332 0.0 6 323 8 0 1 233 300 0.0 6 295 5 234 254 0.0 6 252 2 235 255 0.0 6 245 9 0 1 236 437 0.0 6 430 7 237 793 0.0 6 771 22 238 927 0.0 6 904 23 239 759 0.0 6 746 12 1 240 607 0.0 6 591 16 241 778 0.0 6 760 17 1 242 732 0.0 6 718 13 0 0 1 243 472 0.0 6 466 5 1 244 482 0.0 6 473 9 245 593 0.0 6 579 14 246 542 0.0 6 532 10 247 540 0.0 6 527 13 248 563 0.0 6 552 11 249 568 0.0 6 552 15 1 250 714 0.0 6 691 22 1 251 632 0.0 6 618 14 252 371 0.0 6 362 9 253 433 0.0 6 425 8 254 359 0.0 6 353 6 255 641 0.0 6 626 15 256 785 0.0 6 769 15 1 257 592 0.0 6 578 14 258 608 0.0 6 593 15 259 1077 0.0 6 1050 24 2 1 260 1047 0.0 6 1022 22 2 0 0 1 261 468 0.0 6 458 8 1 0 1 262 389 0.0 6 382 6 1 263 386 0.0 6 377 9 264 415 0.0 6 404 10 1 265 716 0.0 6 703 12 1 266 652 0.0 6 639 13 267 389 0.0 6 377 12 268 1537 0.0 6 1504 33 269 1595 0.0 6 1553 41 1 270 677 0.0 6 665 11 0 1 271 558 0.0 6 539 19 272 594 0.0 6 576 17 1 273 1883 0.0 6 1850 33 274 1960 0.0 6 1927 30 2 1 275 1109 0.0 6 1078 27 2 0 2 276 1573 0.0 6 1529 43 1 277 1787 0.0 6 1692 93 2 278 22608 0.0 6 22060 537 10 1 279 966 0.0 6 879 84 2 1 280 32 0.0 6 30 1 0 0 0 1 281 8 0.0 6 6 1 1 282 5 0.0 6 3 1 0 0 0 1 283 3 0.0 6 3 284 2 0.0 6 2 286 1 0.0 6 1 287 1 0.0 6 1 288 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 166 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 1 0.0 6 1 62 1 0.0 6 1 63 2 0.0 6 2 73 2 0.0 6 2 74 3 0.0 6 3 75 4 0.0 6 4 76 3 0.0 6 3 77 2 0.0 6 2 78 1 0.0 6 1 80 1 0.0 6 1 82 1 0.0 6 1 83 2 0.0 6 2 84 2 0.0 6 2 86 1 0.0 6 1 87 2 0.0 6 2 88 1 0.0 6 1 89 1 0.0 6 1 90 1 0.0 6 1 92 2 0.0 6 2 95 2 0.0 6 2 97 1 0.0 6 1 99 1 0.0 6 1 100 3 0.0 6 3 104 3 0.0 6 3 107 1 0.0 6 1 108 1 0.0 6 1 109 1 0.0 6 1 110 2 0.0 6 2 111 3 0.0 6 3 112 2 0.0 6 2 114 1 0.0 6 1 116 1 0.0 6 1 117 1 0.0 6 1 120 1 0.0 6 1 121 1 0.0 6 1 123 1 0.0 6 1 124 2 0.0 6 2 125 2 0.0 6 2 126 3 0.0 6 3 127 2 0.0 6 2 128 1 0.0 6 1 129 1 0.0 6 1 132 1 0.0 6 1 133 1 0.0 6 1 134 1 0.0 6 1 136 1 0.0 6 1 137 1 0.0 6 1 143 1 0.0 6 1 146 1 0.0 6 1 147 1 0.0 6 1 150 1 0.0 6 1 154 1 0.0 6 1 163 2 0.0 6 2 164 1 0.0 6 1 165 1 0.0 6 1 169 1 0.0 6 1 170 2 0.0 6 2 178 1 0.0 6 1 179 2 0.0 6 2 181 2 0.0 6 2 186 2 0.0 6 2 187 2 0.0 6 2 188 2 0.0 6 2 189 3 0.0 6 3 191 1 0.0 6 1 192 1 0.0 6 1 196 1 0.0 6 1 199 1 0.0 6 1 200 1 0.0 6 1 201 1 0.0 6 1 202 2 0.0 6 2 203 2 0.0 6 2 204 2 0.0 6 2 209 2 0.0 6 2 210 1 0.0 6 1 211 1 0.0 6 1 212 2 0.0 6 2 213 1 0.0 6 1 214 2 0.0 6 2 215 1 0.0 6 1 217 1 0.0 6 1 219 3 0.0 6 3 220 3 0.0 6 3 221 2 0.0 6 2 222 1 0.0 6 1 226 1 0.0 6 1 227 2 0.0 6 2 228 4 0.0 6 4 232 1 0.0 6 1 234 1 0.0 6 1 238 1 0.0 6 1 239 2 0.0 6 2 244 1 0.0 6 1 245 1 0.0 6 1 246 1 0.0 6 1 247 1 0.0 6 1 249 2 0.0 6 2 251 1 0.0 6 1 254 1 0.0 6 1 256 1 0.0 6 1 257 1 0.0 6 1 258 1 0.0 6 1 259 2 0.0 6 2 264 2 0.0 6 2 266 1 0.0 6 1 272 1 0.0 6 1 278 1 0.0 6 1 282 1 0.0 6 1 283 1 0.0 6 1 293 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 80426 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 9 1 0.3 2 1 11 3 0.0 3 3 12 2 0.0 3 2 13 12 0.0 3 8 4 14 10 0.0 4 10 16 14 0.0 4 13 1 17 9 0.0 5 8 1 18 7 0.0 5 6 0 1 19 19 0.0 5 19 20 82 0.0 6 77 3 2 21 69 0.0 6 63 5 1 22 30 0.0 6 29 1 23 25 0.0 6 20 5 24 14 0.0 6 13 1 25 20 0.0 6 18 2 26 12 0.0 6 11 0 1 27 5 0.0 6 5 28 10 0.0 6 9 1 29 11 0.0 6 10 0 1 30 17 0.0 6 15 2 31 8 0.0 6 8 32 10 0.0 6 9 1 33 7 0.0 6 4 3 34 11 0.0 6 10 1 35 7 0.0 6 7 36 9 0.0 6 7 2 37 12 0.0 6 12 38 15 0.0 6 15 39 9 0.0 6 8 1 40 6 0.0 6 6 41 8 0.0 6 8 42 6 0.0 6 6 43 7 0.0 6 7 44 16 0.0 6 16 45 21 0.0 6 21 46 18 0.0 6 16 2 47 16 0.0 6 13 2 1 48 10 0.0 6 9 1 49 22 0.0 6 21 1 50 21 0.0 6 20 1 51 8 0.0 6 8 52 28 0.0 6 23 4 1 53 16 0.0 6 14 2 54 30 0.0 6 28 2 55 17 0.0 6 16 1 56 32 0.0 6 30 2 57 18 0.0 6 15 2 1 58 15 0.0 6 11 4 59 20 0.0 6 20 60 27 0.0 6 26 1 61 18 0.0 6 17 1 62 19 0.0 6 18 1 63 15 0.0 6 13 2 64 18 0.0 6 16 2 65 14 0.0 6 12 2 66 27 0.0 6 22 5 67 37 0.0 6 32 5 68 59 0.0 6 56 3 69 161 0.0 6 154 7 70 35 0.0 6 33 2 71 67 0.0 6 64 3 72 59 0.0 6 52 7 73 39 0.0 6 35 4 74 26 0.0 6 24 1 0 1 75 22 0.0 6 21 1 76 43 0.0 6 41 2 77 32 0.0 6 31 1 78 41 0.0 6 39 1 0 0 1 79 31 0.0 6 29 1 0 0 1 80 22 0.0 6 17 5 81 26 0.0 6 25 1 82 43 0.0 6 38 5 83 63 0.0 6 58 5 84 73 0.0 6 70 3 85 56 0.0 6 51 4 1 86 24 0.0 6 22 2 87 52 0.0 6 47 5 88 27 0.0 6 27 89 44 0.0 6 37 4 1 0 1 1 90 53 0.0 6 52 1 91 55 0.0 6 54 1 92 30 0.0 6 26 4 93 64 0.0 6 62 2 94 95 0.0 6 89 6 95 46 0.0 6 43 3 96 60 0.0 6 53 6 0 0 1 97 59 0.0 6 53 6 98 57 0.0 6 55 2 99 69 0.0 6 66 3 100 65 0.0 6 61 3 1 101 56 0.0 6 54 2 102 79 0.0 6 73 5 1 103 90 0.0 6 87 3 104 106 0.0 6 103 2 1 105 96 0.0 6 90 5 1 106 82 0.0 6 79 3 107 78 0.0 6 73 4 1 108 79 0.0 6 77 2 109 77 0.0 6 72 5 110 76 0.0 6 71 5 111 71 0.0 6 65 6 112 67 0.0 6 60 5 1 0 1 113 68 0.0 6 66 1 1 114 103 0.0 6 98 4 1 115 97 0.0 6 93 4 116 79 0.0 6 75 4 117 64 0.0 6 59 4 0 0 1 118 96 0.0 6 91 5 119 131 0.0 6 127 4 120 178 0.0 6 172 6 121 85 0.0 6 83 2 122 95 0.0 6 92 3 123 120 0.0 6 116 4 124 131 0.0 6 127 4 125 95 0.0 6 93 2 126 83 0.0 6 81 2 127 110 0.0 6 105 4 1 128 82 0.0 6 80 1 1 129 87 0.0 6 85 1 0 0 1 130 82 0.0 6 77 5 131 153 0.0 6 149 4 132 149 0.0 6 146 2 0 0 1 133 126 0.0 6 123 3 134 205 0.0 6 202 3 135 384 0.0 6 372 12 136 247 0.0 6 236 10 1 137 269 0.0 6 258 10 1 138 151 0.0 6 147 3 0 1 139 227 0.0 6 216 10 0 0 0 1 140 349 0.0 6 337 11 1 141 407 0.0 6 388 18 1 142 484 0.0 6 463 18 1 0 2 143 396 0.0 6 385 11 144 573 0.0 6 553 20 145 342 0.0 6 332 10 146 352 0.0 6 337 14 0 1 147 236 0.0 6 221 14 1 148 322 0.0 6 318 3 0 1 149 477 0.0 6 465 11 1 150 662 0.0 6 644 15 1 0 1 1 151 364 0.0 6 341 18 2 1 2 152 960 0.0 6 932 24 1 2 1 153 482 0.0 6 466 15 0 0 1 154 669 0.0 6 650 19 155 571 0.0 6 558 13 156 483 0.0 6 466 16 1 157 635 0.0 6 617 15 1 2 158 605 0.0 6 590 13 0 0 1 1 159 420 0.0 6 408 11 0 0 1 160 613 0.0 6 599 14 161 401 0.0 6 392 9 162 390 0.0 6 380 10 163 533 0.0 6 525 8 164 477 0.0 6 457 20 165 666 0.0 6 641 25 166 630 0.0 6 611 17 1 0 1 167 424 0.0 6 417 7 168 659 0.0 6 646 10 1 0 1 1 169 303 0.0 6 291 12 170 465 0.0 6 450 12 2 1 171 552 0.0 6 525 26 1 172 393 0.0 6 383 9 0 1 173 612 0.0 6 587 20 4 0 0 1 174 771 0.0 6 738 29 2 0 1 1 175 1472 0.0 6 1428 42 1 1 176 900 0.0 6 874 24 0 1 1 177 819 0.0 6 803 14 1 0 1 178 764 0.0 6 742 22 179 817 0.0 6 795 22 180 888 0.0 6 862 24 1 1 181 856 0.0 6 831 22 2 0 0 0 1 182 456 0.0 6 433 20 0 0 2 1 183 931 0.0 6 900 30 0 1 184 199 0.0 6 192 6 1 185 531 0.0 6 517 14 186 1714 0.0 6 1673 39 0 1 0 1 187 1518 0.0 6 1481 35 0 0 1 1 188 1569 0.0 6 1523 41 3 0 2 189 1190 0.0 6 1153 37 190 2107 0.0 6 2041 63 2 0 1 191 1677 0.0 6 1627 45 4 0 1 192 1150 0.0 6 1117 31 0 0 1 1 193 725 0.0 6 702 20 2 0 0 1 194 1316 0.0 6 1256 53 4 0 3 195 2479 0.0 6 2417 58 2 2 196 1517 0.0 6 1471 43 0 2 1 197 1122 0.0 6 1075 42 1 1 3 198 3677 0.0 6 3577 98 1 0 1 199 2492 0.0 6 2428 61 1 0 2 200 1900 0.0 6 1845 53 0 1 1 201 1466 0.0 6 1429 35 1 0 1 202 917 0.0 6 890 24 2 0 1 203 1095 0.0 6 1064 30 1 204 950 0.0 6 932 17 1 205 540 0.0 6 524 14 0 0 2 206 351 0.0 6 347 4 207 391 0.0 6 378 13 208 530 0.0 6 513 14 1 0 2 209 386 0.0 6 375 10 1 210 514 0.0 6 494 18 1 0 1 211 525 0.0 6 512 13 212 278 0.0 6 268 9 0 0 0 0 1 213 261 0.0 6 251 9 1 214 330 0.0 6 324 3 2 0 0 0 1 215 556 0.0 6 532 23 1 216 1047 0.0 6 1018 27 1 1 217 708 0.0 6 693 13 2 218 648 0.0 6 631 17 219 588 0.0 6 568 17 1 2 220 841 0.0 6 818 23 221 459 0.0 6 446 12 0 0 1 222 420 0.0 6 401 19 223 612 0.0 6 601 11 224 746 0.0 6 724 21 0 1 225 715 0.0 6 698 17 226 550 0.0 6 535 15 227 307 0.0 6 300 5 0 1 1 228 317 0.0 6 310 7 229 348 0.0 6 343 5 230 287 0.0 6 283 3 1 231 309 0.0 6 299 10 232 350 0.0 6 338 12 233 314 0.0 6 310 4 234 201 0.0 6 196 5 235 80 0.0 6 77 3 236 91 0.0 6 89 2 237 52 0.0 6 50 2 238 36 0.0 6 36 239 49 0.0 6 49 240 143 0.0 6 139 4 241 198 0.0 6 189 9 242 132 0.0 6 129 3 243 235 0.0 6 227 7 0 0 1 244 147 0.0 6 144 2 0 1 245 105 0.0 6 103 2 246 62 0.0 6 60 2 247 74 0.0 6 72 2 248 62 0.0 6 60 2 249 36 0.0 6 34 1 0 1 250 52 0.0 6 51 1 251 14 0.0 6 14 252 5 0.0 6 4 1 253 20 0.0 6 19 0 1 254 5 0.0 6 5 256 3 0.0 6 3 257 1 0.0 6 1 278 1 0.0 6 1 279 3 0.0 6 3 280 30 0.0 6 30 281 146 0.0 6 143 2 1 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 108 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 54 1 0.0 6 1 69 2 0.0 6 2 70 1 0.0 6 1 72 1 0.0 6 1 73 1 0.0 6 1 74 2 0.0 6 2 75 3 0.0 6 3 76 1 0.0 6 1 78 1 0.0 6 1 79 1 0.0 6 1 80 2 0.0 6 2 81 2 0.0 6 2 83 1 0.0 6 1 84 1 0.0 6 1 86 1 0.0 6 1 87 1 0.0 6 1 89 1 0.0 6 1 90 1 0.0 6 1 91 3 0.0 6 3 92 2 0.0 6 2 93 2 0.0 6 2 97 1 0.0 6 1 98 1 0.0 6 1 100 2 0.0 6 2 102 1 0.0 6 1 104 1 0.0 6 1 105 1 0.0 6 1 108 4 0.0 6 4 109 1 0.0 6 1 110 1 0.0 6 1 112 1 0.0 6 1 113 3 0.0 6 3 117 1 0.0 6 1 118 2 0.0 6 2 121 1 0.0 6 1 123 1 0.0 6 1 126 2 0.0 6 2 127 2 0.0 6 2 128 1 0.0 6 1 130 1 0.0 6 1 131 2 0.0 6 2 132 3 0.0 6 3 133 1 0.0 6 1 136 1 0.0 6 1 137 1 0.0 6 1 138 3 0.0 6 3 140 3 0.0 6 3 142 1 0.0 6 1 143 1 0.0 6 1 144 1 0.0 6 1 145 1 0.0 6 1 146 1 0.0 6 1 147 1 0.0 6 1 148 2 0.0 6 2 149 1 0.0 6 1 151 1 0.0 6 1 152 1 0.0 6 1 157 1 0.0 6 1 159 1 0.0 6 1 161 2 0.0 6 2 162 1 0.0 6 1 164 2 0.0 6 1 0 1 168 1 0.0 6 1 169 3 0.0 6 3 171 2 0.0 6 2 175 1 0.0 6 1 179 1 0.0 6 1 183 1 0.0 6 1 187 1 0.0 6 1 188 1 0.0 6 1 189 1 0.0 6 1 193 1 0.0 6 1 197 1 0.0 6 1 199 2 0.0 6 2 220 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_02_109_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_02_336_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_02_109_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_02_336_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 6.47 s (66 µs/read; 0.91 M reads/minute). === Summary === Total read pairs processed: 98,391 Read 1 with adapter: 98,384 (100.0%) Read 2 with adapter: 98,391 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 7 (0.0%) Pairs written (passing filters): 98,384 (100.0%) Total basepairs processed: 49,877,320 bp Read 1: 27,916,834 bp Read 2: 21,960,486 bp Total written (filtered): 6,195,570 bp (12.4%) Read 1: 3,147,624 bp Read 2: 3,047,946 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 98381 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1537.4 0 0 1 4 1 384.3 1 0 1 6 1 24.0 1 0 0 1 7 2 6.0 2 0 0 2 9 2 0.4 2 2 15 1 0.0 4 1 16 3 0.0 4 2 1 17 1 0.0 5 1 18 1 0.0 5 0 1 20 15 0.0 6 14 1 21 7 0.0 6 6 1 22 7 0.0 6 6 1 24 5 0.0 6 5 25 10 0.0 6 10 26 6 0.0 6 6 27 3 0.0 6 3 28 4 0.0 6 3 1 29 1 0.0 6 1 30 5 0.0 6 5 31 2 0.0 6 2 32 1 0.0 6 1 34 5 0.0 6 4 1 35 1 0.0 6 1 36 2 0.0 6 2 37 4 0.0 6 2 0 0 0 1 0 1 38 6 0.0 6 6 39 1 0.0 6 1 40 3 0.0 6 3 42 4 0.0 6 4 44 10 0.0 6 10 45 7 0.0 6 7 46 4 0.0 6 3 0 1 47 4 0.0 6 3 1 48 2 0.0 6 2 49 4 0.0 6 3 0 1 50 4 0.0 6 4 51 2 0.0 6 0 1 0 1 53 5 0.0 6 5 54 4 0.0 6 4 55 1 0.0 6 1 56 4 0.0 6 4 57 2 0.0 6 2 58 1 0.0 6 1 60 3 0.0 6 2 1 61 1 0.0 6 1 62 2 0.0 6 2 63 2 0.0 6 1 1 64 9 0.0 6 7 2 65 2 0.0 6 1 1 67 3 0.0 6 3 68 2 0.0 6 1 1 69 2 0.0 6 2 70 3 0.0 6 2 1 72 4 0.0 6 4 73 1 0.0 6 1 76 1 0.0 6 1 77 5 0.0 6 4 1 78 2 0.0 6 2 79 1 0.0 6 1 80 1 0.0 6 1 81 3 0.0 6 2 1 82 2 0.0 6 2 83 2 0.0 6 2 84 1 0.0 6 1 85 2 0.0 6 2 86 11 0.0 6 11 87 10 0.0 6 9 1 88 1 0.0 6 1 89 11 0.0 6 11 90 3 0.0 6 3 91 3 0.0 6 3 92 11 0.0 6 10 1 93 2 0.0 6 1 1 94 2 0.0 6 2 95 1 0.0 6 1 96 1 0.0 6 1 97 6 0.0 6 5 1 98 15 0.0 6 14 1 100 9 0.0 6 9 101 19 0.0 6 18 1 102 8 0.0 6 7 1 103 6 0.0 6 6 104 3 0.0 6 3 105 5 0.0 6 4 1 106 5 0.0 6 3 1 0 1 107 11 0.0 6 9 1 1 108 13 0.0 6 12 1 109 7 0.0 6 7 110 2 0.0 6 0 2 111 4 0.0 6 4 112 13 0.0 6 13 113 6 0.0 6 6 114 6 0.0 6 5 0 1 115 21 0.0 6 20 1 116 9 0.0 6 9 117 11 0.0 6 10 1 118 5 0.0 6 5 119 12 0.0 6 9 2 1 120 4 0.0 6 4 121 13 0.0 6 12 1 122 15 0.0 6 14 1 123 17 0.0 6 16 1 124 10 0.0 6 9 1 125 13 0.0 6 13 126 18 0.0 6 17 1 127 12 0.0 6 10 1 1 128 26 0.0 6 25 1 129 19 0.0 6 17 1 1 130 14 0.0 6 14 131 16 0.0 6 15 1 132 21 0.0 6 19 2 133 41 0.0 6 39 2 134 104 0.0 6 101 3 135 61 0.0 6 58 3 136 28 0.0 6 27 1 137 14 0.0 6 14 138 11 0.0 6 11 139 9 0.0 6 9 140 25 0.0 6 23 2 141 26 0.0 6 25 1 142 34 0.0 6 33 1 143 12 0.0 6 11 1 144 15 0.0 6 15 145 17 0.0 6 16 1 146 21 0.0 6 20 1 147 8 0.0 6 7 1 148 35 0.0 6 34 1 149 41 0.0 6 39 2 150 36 0.0 6 36 151 29 0.0 6 27 2 152 31 0.0 6 30 1 153 44 0.0 6 41 3 154 20 0.0 6 19 1 155 25 0.0 6 25 156 7 0.0 6 7 157 34 0.0 6 34 158 9 0.0 6 9 159 17 0.0 6 15 2 160 26 0.0 6 25 1 161 28 0.0 6 27 1 162 28 0.0 6 25 2 1 163 26 0.0 6 25 1 164 20 0.0 6 20 165 35 0.0 6 34 1 166 38 0.0 6 35 3 167 82 0.0 6 81 1 168 66 0.0 6 64 2 169 12 0.0 6 10 2 170 46 0.0 6 45 1 171 85 0.0 6 82 3 172 25 0.0 6 24 1 173 42 0.0 6 41 1 174 20 0.0 6 20 175 31 0.0 6 31 176 29 0.0 6 26 2 1 177 36 0.0 6 34 1 1 178 3 0.0 6 3 179 30 0.0 6 28 1 1 180 132 0.0 6 125 7 181 61 0.0 6 58 3 182 45 0.0 6 45 183 34 0.0 6 33 1 184 66 0.0 6 63 3 185 121 0.0 6 119 2 186 34 0.0 6 34 187 38 0.0 6 38 188 78 0.0 6 76 2 189 109 0.0 6 107 1 1 190 163 0.0 6 154 9 191 233 0.0 6 225 7 1 192 106 0.0 6 100 5 1 193 89 0.0 6 85 3 0 1 194 113 0.0 6 111 2 195 122 0.0 6 118 3 1 196 128 0.0 6 127 1 197 81 0.0 6 80 1 198 85 0.0 6 82 2 1 199 92 0.0 6 89 3 200 146 0.0 6 140 6 201 204 0.0 6 200 4 202 166 0.0 6 164 2 203 107 0.0 6 104 2 0 1 204 130 0.0 6 122 8 205 325 0.0 6 314 11 206 466 0.0 6 459 7 207 333 0.0 6 324 9 208 837 0.0 6 811 24 1 1 209 412 0.0 6 399 13 210 501 0.0 6 481 20 211 1012 0.0 6 993 18 1 212 246 0.0 6 234 12 213 1083 0.0 6 1056 25 1 0 1 214 190 0.0 6 181 9 215 334 0.0 6 325 9 216 252 0.0 6 241 11 217 455 0.0 6 442 13 218 188 0.0 6 178 10 219 503 0.0 6 492 10 1 220 265 0.0 6 248 16 0 1 221 780 0.0 6 766 14 222 223 0.0 6 207 16 223 1513 0.0 6 1484 26 3 224 591 0.0 6 574 16 1 225 812 0.0 6 801 11 226 1096 0.0 6 1074 20 1 0 0 1 227 507 0.0 6 493 12 1 1 228 645 0.0 6 637 8 229 355 0.0 6 345 10 230 345 0.0 6 337 8 231 345 0.0 6 336 9 232 284 0.0 6 281 3 233 361 0.0 6 347 14 234 541 0.0 6 537 4 235 215 0.0 6 211 4 236 416 0.0 6 393 23 237 1933 0.0 6 1893 39 1 238 226 0.0 6 220 6 239 405 0.0 6 397 8 240 242 0.0 6 236 6 241 206 0.0 6 202 4 242 234 0.0 6 227 7 243 717 0.0 6 695 22 244 1414 0.0 6 1392 21 1 245 463 0.0 6 457 6 246 327 0.0 6 313 12 1 1 247 852 0.0 6 836 14 1 1 248 709 0.0 6 668 39 2 249 2971 0.0 6 2908 62 1 250 1047 0.0 6 1022 23 2 251 922 0.0 6 894 27 1 252 1167 0.0 6 1136 29 2 253 2291 0.0 6 2231 57 1 2 254 2416 0.0 6 2370 46 255 1926 0.0 6 1857 69 256 5041 0.0 6 4956 81 3 1 257 1441 0.0 6 1390 49 1 0 1 258 4435 0.0 6 4360 75 259 1554 0.0 6 1501 51 1 0 1 260 5371 0.0 6 5287 84 261 462 0.0 6 448 13 0 0 1 262 362 0.0 6 350 12 263 985 0.0 6 956 29 264 1254 0.0 6 1233 20 1 265 445 0.0 6 421 24 266 1257 0.0 6 1242 14 1 267 605 0.0 6 595 10 268 1110 0.0 6 1081 28 1 269 319 0.0 6 304 15 270 641 0.0 6 625 16 271 418 0.0 6 408 9 1 272 670 0.0 6 656 13 0 1 273 2033 0.0 6 1990 42 1 274 1517 0.0 6 1476 37 4 275 3874 0.0 6 3779 91 4 276 5560 0.0 6 5450 106 4 277 2971 0.0 6 2887 80 3 1 278 14111 0.0 6 13872 233 4 2 279 195 0.0 6 178 13 3 1 280 20 0.0 6 19 0 1 281 8 0.0 6 5 1 1 1 282 8 0.0 6 8 283 1 0.0 6 1 285 5 0.0 6 5 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 3 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 74 1 0.0 6 1 78 1 0.0 6 1 115 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 98304 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 6.0 2 1 10 2 0.1 3 2 11 3 0.0 3 3 12 6 0.0 3 5 1 13 11 0.0 3 10 1 14 4 0.0 4 4 15 3 0.0 4 3 16 7 0.0 4 7 17 22 0.0 5 21 1 18 14 0.0 5 12 1 1 19 6 0.0 5 6 20 12 0.0 6 12 21 31 0.0 6 29 2 22 37 0.0 6 36 1 23 23 0.0 6 22 1 24 19 0.0 6 17 2 25 21 0.0 6 20 1 26 22 0.0 6 17 5 27 13 0.0 6 11 2 28 6 0.0 6 5 1 29 17 0.0 6 14 3 30 7 0.0 6 5 2 31 8 0.0 6 8 32 10 0.0 6 10 33 6 0.0 6 5 1 34 13 0.0 6 11 1 0 0 1 35 11 0.0 6 9 2 36 3 0.0 6 3 37 7 0.0 6 7 38 16 0.0 6 16 39 13 0.0 6 12 1 40 12 0.0 6 11 1 41 5 0.0 6 5 42 6 0.0 6 4 2 43 9 0.0 6 8 1 44 27 0.0 6 26 1 45 11 0.0 6 9 2 46 31 0.0 6 28 3 47 20 0.0 6 17 3 48 6 0.0 6 5 1 49 31 0.0 6 28 3 50 15 0.0 6 13 2 51 13 0.0 6 12 1 52 27 0.0 6 25 2 53 23 0.0 6 22 1 54 35 0.0 6 33 1 1 55 12 0.0 6 10 1 1 56 17 0.0 6 15 2 57 36 0.0 6 32 3 1 58 22 0.0 6 20 2 59 20 0.0 6 20 60 18 0.0 6 15 3 61 16 0.0 6 15 0 1 62 9 0.0 6 8 1 63 10 0.0 6 8 2 64 10 0.0 6 10 65 6 0.0 6 4 1 1 66 15 0.0 6 13 2 67 49 0.0 6 46 3 68 173 0.0 6 168 5 69 40 0.0 6 36 4 70 62 0.0 6 58 4 71 31 0.0 6 28 3 72 63 0.0 6 59 4 73 11 0.0 6 11 74 13 0.0 6 12 1 75 15 0.0 6 12 3 76 28 0.0 6 26 2 77 21 0.0 6 18 3 78 40 0.0 6 39 1 79 31 0.0 6 28 3 80 35 0.0 6 33 2 81 14 0.0 6 12 2 82 16 0.0 6 15 1 83 101 0.0 6 93 8 84 93 0.0 6 87 6 85 39 0.0 6 39 86 37 0.0 6 35 1 1 87 65 0.0 6 60 5 88 22 0.0 6 19 2 1 89 29 0.0 6 28 1 90 37 0.0 6 34 3 91 35 0.0 6 32 3 92 18 0.0 6 14 4 93 42 0.0 6 39 3 94 139 0.0 6 131 8 95 47 0.0 6 43 2 2 96 41 0.0 6 37 2 2 97 31 0.0 6 31 98 36 0.0 6 32 4 99 54 0.0 6 51 3 100 51 0.0 6 48 3 101 61 0.0 6 58 3 102 73 0.0 6 68 4 1 103 94 0.0 6 90 4 104 78 0.0 6 75 2 0 1 105 138 0.0 6 136 2 106 59 0.0 6 54 3 1 0 1 107 41 0.0 6 39 2 108 47 0.0 6 44 3 109 52 0.0 6 50 2 110 64 0.0 6 61 2 1 111 60 0.0 6 55 5 112 44 0.0 6 42 2 113 46 0.0 6 43 2 1 114 73 0.0 6 71 2 115 47 0.0 6 45 2 116 63 0.0 6 58 4 1 117 21 0.0 6 21 118 56 0.0 6 51 4 0 0 1 119 66 0.0 6 66 120 94 0.0 6 86 7 1 121 49 0.0 6 44 5 122 66 0.0 6 63 3 123 59 0.0 6 55 3 1 124 88 0.0 6 85 3 125 78 0.0 6 74 4 126 50 0.0 6 46 4 127 108 0.0 6 101 7 128 50 0.0 6 47 2 1 129 40 0.0 6 35 4 1 130 40 0.0 6 38 2 131 124 0.0 6 116 6 0 1 1 132 152 0.0 6 147 5 133 74 0.0 6 69 4 1 134 181 0.0 6 174 7 135 358 0.0 6 347 11 136 136 0.0 6 131 5 137 252 0.0 6 243 9 138 76 0.0 6 74 2 139 167 0.0 6 163 4 140 372 0.0 6 365 7 141 270 0.0 6 262 7 0 1 142 357 0.0 6 343 13 1 143 420 0.0 6 409 8 2 1 144 515 0.0 6 501 13 1 145 309 0.0 6 298 11 146 319 0.0 6 302 15 1 1 147 362 0.0 6 351 10 1 148 322 0.0 6 313 8 1 149 377 0.0 6 363 10 2 0 2 150 460 0.0 6 447 13 151 486 0.0 6 473 13 152 313 0.0 6 305 8 153 406 0.0 6 398 7 1 154 368 0.0 6 359 8 1 155 203 0.0 6 195 6 0 2 156 351 0.0 6 345 4 1 1 157 456 0.0 6 442 14 158 543 0.0 6 535 8 159 246 0.0 6 241 4 1 160 380 0.0 6 369 10 1 161 278 0.0 6 273 5 162 150 0.0 6 148 2 163 425 0.0 6 412 11 1 1 164 570 0.0 6 552 18 165 571 0.0 6 560 10 0 0 1 166 797 0.0 6 791 6 167 421 0.0 6 405 15 0 1 168 1138 0.0 6 1113 25 169 438 0.0 6 423 14 1 170 581 0.0 6 564 15 1 0 1 171 770 0.0 6 753 17 172 265 0.0 6 258 7 173 464 0.0 6 449 15 174 685 0.0 6 670 13 0 0 1 1 175 1321 0.0 6 1304 16 0 0 1 176 798 0.0 6 768 27 2 0 0 1 177 1301 0.0 6 1275 25 1 178 946 0.0 6 922 23 0 1 179 1195 0.0 6 1174 21 180 1048 0.0 6 1025 20 2 0 0 0 1 181 1447 0.0 6 1416 29 1 0 1 182 350 0.0 6 339 9 1 0 1 183 1452 0.0 6 1430 21 0 0 0 1 184 1185 0.0 6 1163 19 2 0 0 1 185 876 0.0 6 849 26 0 0 1 186 2618 0.0 6 2560 51 2 0 4 0 1 187 1829 0.0 6 1783 44 1 0 1 188 2427 0.0 6 2384 41 1 0 1 189 1136 0.0 6 1099 35 1 1 190 1376 0.0 6 1337 38 0 0 0 1 191 1211 0.0 6 1172 37 1 0 1 192 1168 0.0 6 1131 36 0 1 193 2239 0.0 6 2191 46 1 1 194 558 0.0 6 539 18 1 195 1637 0.0 6 1600 33 1 1 2 196 370 0.0 6 359 7 4 197 1808 0.0 6 1776 29 0 0 3 198 2647 0.0 6 2581 65 0 0 1 199 1737 0.0 6 1705 30 2 200 1318 0.0 6 1295 20 0 1 2 201 1436 0.0 6 1394 38 1 0 3 202 1305 0.0 6 1285 19 1 203 1084 0.0 6 1070 13 0 1 204 748 0.0 6 721 26 1 205 1610 0.0 6 1572 33 1 0 4 206 296 0.0 6 287 8 1 207 1056 0.0 6 1035 21 208 1881 0.0 6 1842 38 0 1 209 1389 0.0 6 1369 17 2 0 1 210 369 0.0 6 355 13 0 1 211 773 0.0 6 748 24 1 212 1015 0.0 6 983 32 213 1479 0.0 6 1444 34 0 0 0 1 214 555 0.0 6 538 17 215 750 0.0 6 729 20 0 0 1 216 576 0.0 6 567 8 1 217 493 0.0 6 486 6 1 218 541 0.0 6 531 10 219 495 0.0 6 479 13 2 0 1 220 226 0.0 6 222 3 0 0 1 221 152 0.0 6 146 4 1 1 222 436 0.0 6 418 14 1 2 1 223 1213 0.0 6 1177 32 3 0 1 224 2346 0.0 6 2298 47 0 0 1 225 1420 0.0 6 1389 27 2 1 1 226 1174 0.0 6 1131 42 1 227 4333 0.0 6 4249 77 3 1 3 228 1220 0.0 6 1190 30 229 2440 0.0 6 2404 35 0 0 1 230 157 0.0 6 151 5 0 1 231 513 0.0 6 504 9 232 623 0.0 6 608 14 0 0 1 233 987 0.0 6 965 19 3 234 719 0.0 6 699 20 235 421 0.0 6 413 8 236 186 0.0 6 181 5 237 291 0.0 6 288 3 238 358 0.0 6 352 5 1 239 164 0.0 6 158 6 240 53 0.0 6 51 1 1 241 44 0.0 6 41 3 242 720 0.0 6 715 5 243 422 0.0 6 409 12 0 0 1 244 284 0.0 6 276 8 245 179 0.0 6 176 3 246 259 0.0 6 253 6 247 165 0.0 6 161 4 248 284 0.0 6 281 3 249 396 0.0 6 384 12 250 88 0.0 6 84 4 251 297 0.0 6 289 7 1 252 489 0.0 6 477 11 1 253 48 0.0 6 45 3 254 29 0.0 6 29 255 22 0.0 6 21 1 256 3 0.0 6 3 257 3 0.0 6 3 261 1 0.0 6 1 263 1 0.0 6 1 264 2 0.0 6 2 266 2 0.0 6 2 267 1 0.0 6 1 274 1 0.0 6 1 277 1 0.0 6 1 278 6 0.0 6 6 279 33 0.0 6 31 2 280 29 0.0 6 25 4 281 6 0.0 6 4 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 87 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 3 85 1537.4 0 85 82 1 0.0 6 1 141 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_03_110_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_03_337_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_03_110_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_03_337_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.23 s (56 µs/read; 1.07 M reads/minute). === Summary === Total read pairs processed: 93,285 Read 1 with adapter: 93,276 (100.0%) Read 2 with adapter: 93,216 (99.9%) == Read fate breakdown == Pairs that were too short: 14 (0.0%) Pairs discarded as untrimmed: 78 (0.1%) Pairs written (passing filters): 93,193 (99.9%) Total basepairs processed: 43,672,429 bp Read 1: 24,656,086 bp Read 2: 19,016,343 bp Total written (filtered): 6,016,169 bp (13.8%) Read 1: 3,018,547 bp Read 2: 2,997,622 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 92502 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1457.6 0 1 1 4 4 364.4 1 4 6 2 22.8 1 0 0 2 7 3 5.7 2 2 0 1 8 1 1.4 2 1 9 6 0.4 2 5 0 0 1 15 5 0.0 4 5 16 1 0.0 4 0 1 17 2 0.0 5 2 19 3 0.0 5 1 2 20 64 0.0 6 61 3 21 41 0.0 6 40 1 22 30 0.0 6 28 2 23 24 0.0 6 24 24 16 0.0 6 15 1 25 17 0.0 6 17 26 16 0.0 6 14 2 27 7 0.0 6 7 28 7 0.0 6 7 29 5 0.0 6 5 30 3 0.0 6 3 31 3 0.0 6 3 32 8 0.0 6 8 33 6 0.0 6 5 1 34 2 0.0 6 2 35 6 0.0 6 6 36 2 0.0 6 2 37 3 0.0 6 3 38 4 0.0 6 4 39 5 0.0 6 5 40 7 0.0 6 7 41 5 0.0 6 5 42 2 0.0 6 2 43 2 0.0 6 2 44 9 0.0 6 8 1 45 11 0.0 6 10 1 46 7 0.0 6 7 47 3 0.0 6 2 1 48 3 0.0 6 3 49 6 0.0 6 6 50 6 0.0 6 5 0 1 51 7 0.0 6 7 52 7 0.0 6 7 53 4 0.0 6 4 54 10 0.0 6 9 1 55 4 0.0 6 4 56 10 0.0 6 9 0 0 0 1 57 4 0.0 6 4 58 3 0.0 6 3 59 6 0.0 6 6 60 5 0.0 6 5 61 6 0.0 6 6 62 5 0.0 6 5 63 4 0.0 6 4 64 7 0.0 6 6 1 65 8 0.0 6 6 2 66 8 0.0 6 7 1 67 7 0.0 6 7 68 2 0.0 6 2 69 5 0.0 6 4 1 70 3 0.0 6 2 1 71 8 0.0 6 7 0 0 0 1 72 7 0.0 6 7 73 5 0.0 6 4 1 74 7 0.0 6 7 75 9 0.0 6 9 76 6 0.0 6 6 77 9 0.0 6 9 78 6 0.0 6 5 1 79 6 0.0 6 6 80 4 0.0 6 3 1 81 8 0.0 6 8 82 10 0.0 6 9 0 0 0 0 1 83 9 0.0 6 8 1 84 7 0.0 6 4 3 85 8 0.0 6 8 86 11 0.0 6 11 87 5 0.0 6 5 88 8 0.0 6 8 89 11 0.0 6 11 90 8 0.0 6 8 91 6 0.0 6 5 1 92 8 0.0 6 8 93 7 0.0 6 7 94 6 0.0 6 5 1 95 4 0.0 6 2 1 0 0 0 1 96 4 0.0 6 4 97 11 0.0 6 11 98 14 0.0 6 14 99 3 0.0 6 2 1 100 12 0.0 6 11 1 101 16 0.0 6 16 102 7 0.0 6 7 103 12 0.0 6 12 104 11 0.0 6 11 105 14 0.0 6 12 2 106 13 0.0 6 13 107 11 0.0 6 11 108 13 0.0 6 12 1 109 14 0.0 6 13 1 110 14 0.0 6 13 1 111 15 0.0 6 15 112 23 0.0 6 22 1 113 10 0.0 6 10 114 18 0.0 6 18 115 17 0.0 6 16 1 116 12 0.0 6 12 117 12 0.0 6 12 118 15 0.0 6 14 1 119 20 0.0 6 19 1 120 11 0.0 6 10 1 121 14 0.0 6 14 122 26 0.0 6 25 1 123 35 0.0 6 31 4 124 18 0.0 6 17 1 125 23 0.0 6 23 126 24 0.0 6 24 127 18 0.0 6 18 128 36 0.0 6 33 3 129 23 0.0 6 22 1 130 24 0.0 6 20 2 1 0 1 131 12 0.0 6 11 1 132 15 0.0 6 15 133 28 0.0 6 27 1 134 42 0.0 6 41 1 135 30 0.0 6 29 1 136 30 0.0 6 30 137 63 0.0 6 61 2 138 64 0.0 6 64 139 36 0.0 6 34 2 140 56 0.0 6 54 2 141 52 0.0 6 51 0 0 1 142 59 0.0 6 59 143 149 0.0 6 146 3 144 293 0.0 6 291 2 145 385 0.0 6 385 146 228 0.0 6 228 147 187 0.0 6 183 3 1 148 281 0.0 6 277 3 0 0 1 149 260 0.0 6 258 2 150 195 0.0 6 188 7 151 284 0.0 6 281 3 152 226 0.0 6 224 1 1 153 178 0.0 6 177 0 0 1 154 188 0.0 6 186 1 0 0 0 0 1 155 145 0.0 6 145 156 166 0.0 6 164 2 157 183 0.0 6 183 158 188 0.0 6 186 2 159 183 0.0 6 182 1 160 208 0.0 6 204 4 161 195 0.0 6 193 2 162 174 0.0 6 173 1 163 232 0.0 6 228 4 164 213 0.0 6 208 5 165 195 0.0 6 192 2 0 0 0 0 1 166 210 0.0 6 207 3 167 177 0.0 6 175 2 168 186 0.0 6 184 2 169 256 0.0 6 252 4 170 167 0.0 6 166 1 171 179 0.0 6 179 172 215 0.0 6 210 5 173 224 0.0 6 221 2 0 0 1 174 201 0.0 6 199 2 175 307 0.0 6 302 5 176 242 0.0 6 240 2 177 244 0.0 6 242 2 178 212 0.0 6 212 179 256 0.0 6 252 4 180 289 0.0 6 288 1 181 569 0.0 6 567 2 182 349 0.0 6 341 7 1 183 268 0.0 6 266 2 184 376 0.0 6 369 6 1 185 378 0.0 6 372 6 186 461 0.0 6 448 13 187 320 0.0 6 314 6 188 267 0.0 6 259 8 189 308 0.0 6 306 2 190 336 0.0 6 331 5 191 342 0.0 6 338 4 192 363 0.0 6 360 3 193 428 0.0 6 420 8 194 412 0.0 6 406 6 195 462 0.0 6 458 4 196 663 0.0 6 654 9 197 657 0.0 6 647 10 198 713 0.0 6 704 9 199 535 0.0 6 525 9 0 0 1 200 515 0.0 6 510 5 201 550 0.0 6 537 12 1 202 543 0.0 6 531 12 203 676 0.0 6 667 8 1 204 600 0.0 6 593 7 205 837 0.0 6 830 7 206 671 0.0 6 653 17 1 207 770 0.0 6 753 15 1 0 0 1 208 908 0.0 6 890 17 1 209 662 0.0 6 656 6 210 582 0.0 6 573 9 211 577 0.0 6 564 13 212 608 0.0 6 599 9 213 745 0.0 6 734 11 214 720 0.0 6 711 9 215 854 0.0 6 846 7 1 216 637 0.0 6 626 11 217 887 0.0 6 877 9 1 218 758 0.0 6 750 6 1 0 0 1 219 660 0.0 6 653 7 220 532 0.0 6 521 10 0 0 0 1 221 540 0.0 6 533 7 222 763 0.0 6 754 9 223 526 0.0 6 518 8 224 452 0.0 6 442 8 0 0 2 225 752 0.0 6 738 14 226 1299 0.0 6 1273 25 0 0 1 227 945 0.0 6 933 11 1 228 1016 0.0 6 1007 9 229 792 0.0 6 778 12 2 230 942 0.0 6 927 14 0 0 1 231 630 0.0 6 619 11 232 783 0.0 6 769 13 1 233 828 0.0 6 816 11 1 234 730 0.0 6 717 12 1 235 515 0.0 6 507 8 236 481 0.0 6 476 4 0 1 237 445 0.0 6 435 10 238 554 0.0 6 546 6 0 1 1 239 565 0.0 6 552 13 240 829 0.0 6 815 14 241 993 0.0 6 975 17 1 242 921 0.0 6 900 21 243 801 0.0 6 787 14 244 646 0.0 6 635 10 1 245 937 0.0 6 921 16 246 433 0.0 6 428 5 247 490 0.0 6 472 17 1 248 321 0.0 6 312 9 249 863 0.0 6 845 18 250 583 0.0 6 569 14 251 759 0.0 6 739 20 252 645 0.0 6 629 14 2 253 573 0.0 6 556 17 254 1265 0.0 6 1240 25 255 710 0.0 6 693 16 1 256 604 0.0 6 591 13 257 851 0.0 6 829 19 2 0 1 258 926 0.0 6 898 27 0 0 1 259 1114 0.0 6 1094 18 2 260 1357 0.0 6 1334 22 0 1 261 671 0.0 6 654 16 1 262 701 0.0 6 686 15 263 1389 0.0 6 1366 23 264 614 0.0 6 598 14 2 265 992 0.0 6 982 10 266 516 0.0 6 501 13 2 267 885 0.0 6 865 19 1 268 767 0.0 6 751 15 1 269 903 0.0 6 888 14 1 270 507 0.0 6 494 12 1 271 640 0.0 6 623 15 1 1 272 1462 0.0 6 1434 27 0 1 273 1066 0.0 6 1036 29 1 274 1240 0.0 6 1216 24 275 1412 0.0 6 1377 35 276 2348 0.0 6 2303 43 2 277 2535 0.0 6 2485 48 2 278 2586 0.0 6 2527 51 3 2 3 279 8416 0.0 6 8046 352 16 1 1 280 131 0.0 6 115 16 281 24 0.0 6 22 1 1 282 4 0.0 6 2 0 0 0 2 283 2 0.0 6 2 284 2 0.0 6 2 286 1 0.0 6 1 287 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 774 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 25 0.0 6 23 2 71 2 0.0 6 2 72 3 0.0 6 3 73 13 0.0 6 13 74 13 0.0 6 13 75 5 0.0 6 5 76 3 0.0 6 3 77 11 0.0 6 11 78 6 0.0 6 6 79 3 0.0 6 3 80 4 0.0 6 4 81 5 0.0 6 5 82 7 0.0 6 7 83 3 0.0 6 3 84 6 0.0 6 6 85 3 0.0 6 3 86 6 0.0 6 6 87 3 0.0 6 3 89 3 0.0 6 3 90 3 0.0 6 3 91 3 0.0 6 3 92 6 0.0 6 6 93 3 0.0 6 3 94 2 0.0 6 2 95 5 0.0 6 5 96 2 0.0 6 2 97 1 0.0 6 1 98 4 0.0 6 4 99 6 0.0 6 6 100 2 0.0 6 2 101 1 0.0 6 1 102 2 0.0 6 2 103 2 0.0 6 2 104 5 0.0 6 5 105 6 0.0 6 6 106 4 0.0 6 4 107 1 0.0 6 1 108 8 0.0 6 8 109 10 0.0 6 10 110 8 0.0 6 8 112 5 0.0 6 5 113 6 0.0 6 6 115 3 0.0 6 3 116 4 0.0 6 4 117 2 0.0 6 2 118 2 0.0 6 2 119 7 0.0 6 7 120 2 0.0 6 2 121 4 0.0 6 4 122 1 0.0 6 1 123 6 0.0 6 6 124 2 0.0 6 2 125 6 0.0 6 6 126 2 0.0 6 2 127 3 0.0 6 3 128 5 0.0 6 5 129 2 0.0 6 2 130 2 0.0 6 2 131 3 0.0 6 3 132 2 0.0 6 2 133 8 0.0 6 8 134 12 0.0 6 12 135 7 0.0 6 7 136 5 0.0 6 5 137 4 0.0 6 4 138 5 0.0 6 5 139 5 0.0 6 5 140 3 0.0 6 3 141 4 0.0 6 4 142 4 0.0 6 4 143 1 0.0 6 1 144 4 0.0 6 4 145 5 0.0 6 5 146 4 0.0 6 4 147 4 0.0 6 4 148 5 0.0 6 5 149 7 0.0 6 7 150 6 0.0 6 6 151 4 0.0 6 4 152 9 0.0 6 9 153 7 0.0 6 7 154 5 0.0 6 5 155 4 0.0 6 4 156 4 0.0 6 4 157 4 0.0 6 4 158 5 0.0 6 5 159 4 0.0 6 4 160 3 0.0 6 3 161 4 0.0 6 4 162 6 0.0 6 5 0 0 0 0 0 1 163 7 0.0 6 7 164 2 0.0 6 2 165 5 0.0 6 5 166 8 0.0 6 8 167 11 0.0 6 11 168 9 0.0 6 9 169 8 0.0 6 8 170 1 0.0 6 1 171 3 0.0 6 3 172 3 0.0 6 3 173 7 0.0 6 7 174 9 0.0 6 9 175 2 0.0 6 2 176 2 0.0 6 2 177 7 0.0 6 6 1 178 3 0.0 6 3 179 1 0.0 6 1 180 7 0.0 6 7 181 6 0.0 6 6 182 4 0.0 6 4 183 4 0.0 6 4 184 6 0.0 6 6 185 8 0.0 6 8 186 1 0.0 6 1 187 7 0.0 6 7 188 5 0.0 6 5 189 5 0.0 6 5 190 3 0.0 6 3 191 3 0.0 6 3 192 4 0.0 6 4 193 2 0.0 6 2 194 4 0.0 6 4 195 4 0.0 6 4 196 1 0.0 6 1 197 1 0.0 6 1 198 2 0.0 6 2 199 2 0.0 6 2 200 3 0.0 6 3 201 3 0.0 6 3 202 4 0.0 6 4 203 4 0.0 6 4 204 4 0.0 6 4 205 3 0.0 6 3 206 6 0.0 6 6 207 4 0.0 6 4 208 5 0.0 6 5 209 6 0.0 6 6 210 3 0.0 6 3 211 4 0.0 6 4 212 3 0.0 6 3 213 3 0.0 6 2 1 214 4 0.0 6 4 215 5 0.0 6 5 216 5 0.0 6 5 217 1 0.0 6 1 218 6 0.0 6 6 219 3 0.0 6 3 220 5 0.0 6 5 221 5 0.0 6 5 222 5 0.0 6 5 223 6 0.0 6 6 224 2 0.0 6 2 225 2 0.0 6 2 226 3 0.0 6 3 227 3 0.0 6 3 228 3 0.0 6 3 229 3 0.0 6 3 230 2 0.0 6 2 231 4 0.0 6 4 232 3 0.0 6 3 233 2 0.0 6 2 234 2 0.0 6 2 235 1 0.0 6 1 236 1 0.0 6 1 237 2 0.0 6 2 238 3 0.0 6 3 239 1 0.0 6 1 241 5 0.0 6 5 242 2 0.0 6 2 244 1 0.0 6 1 249 1 0.0 6 1 250 2 0.0 6 2 251 4 0.0 6 4 252 1 0.0 6 1 253 2 0.0 6 2 254 1 0.0 6 1 255 2 0.0 6 2 256 1 0.0 6 1 257 1 0.0 6 1 258 1 0.0 6 1 261 2 0.0 6 2 262 2 0.0 6 2 265 1 0.0 6 1 266 1 0.0 6 1 267 1 0.0 6 1 275 1 0.0 6 1 285 1 0.0 6 1 288 1 0.0 6 1 292 1 0.0 6 1 295 1 0.0 6 1 296 1 0.0 6 1 297 1 0.0 6 1 298 1 0.0 6 1 299 1 0.0 6 1 305 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 92619 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1457.6 0 1 8 1 1.4 2 0 0 0 1 10 4 0.1 3 4 11 6 0.0 3 6 12 1 0.0 3 1 13 8 0.0 3 8 14 12 0.0 4 11 0 1 15 3 0.0 4 2 0 1 16 11 0.0 4 8 2 1 17 22 0.0 5 17 5 18 24 0.0 5 22 2 19 18 0.0 5 18 20 93 0.0 6 89 4 21 58 0.0 6 56 1 1 22 42 0.0 6 40 2 23 26 0.0 6 25 1 24 36 0.0 6 33 2 1 25 28 0.0 6 28 26 17 0.0 6 16 1 27 19 0.0 6 18 1 28 15 0.0 6 15 29 16 0.0 6 16 30 29 0.0 6 28 1 31 18 0.0 6 16 2 32 14 0.0 6 13 1 33 13 0.0 6 13 34 14 0.0 6 13 1 35 19 0.0 6 19 36 14 0.0 6 13 1 37 13 0.0 6 11 2 38 8 0.0 6 7 0 1 39 15 0.0 6 14 1 40 14 0.0 6 12 2 41 15 0.0 6 13 2 42 12 0.0 6 11 1 43 19 0.0 6 18 1 44 18 0.0 6 18 45 12 0.0 6 9 2 0 0 0 0 1 46 28 0.0 6 25 2 1 47 30 0.0 6 28 2 48 16 0.0 6 14 1 1 49 26 0.0 6 24 1 0 0 1 50 32 0.0 6 30 2 51 10 0.0 6 9 1 52 25 0.0 6 25 53 41 0.0 6 39 2 54 35 0.0 6 32 3 55 22 0.0 6 19 1 1 0 0 0 1 56 26 0.0 6 23 3 57 35 0.0 6 26 9 58 33 0.0 6 29 2 1 0 1 59 28 0.0 6 26 1 0 0 0 0 1 60 24 0.0 6 21 3 61 23 0.0 6 22 1 62 24 0.0 6 21 1 1 0 1 63 23 0.0 6 21 2 64 36 0.0 6 32 4 65 21 0.0 6 19 2 66 54 0.0 6 47 6 1 67 159 0.0 6 158 1 68 76 0.0 6 72 4 69 81 0.0 6 80 1 70 34 0.0 6 33 1 71 57 0.0 6 56 0 1 72 47 0.0 6 45 2 73 24 0.0 6 21 3 74 41 0.0 6 41 75 42 0.0 6 34 8 76 31 0.0 6 29 2 77 38 0.0 6 34 4 78 36 0.0 6 34 1 0 0 0 1 79 42 0.0 6 40 2 80 33 0.0 6 30 2 1 81 34 0.0 6 31 1 0 1 1 82 54 0.0 6 50 3 1 83 110 0.0 6 107 2 1 84 57 0.0 6 53 4 85 40 0.0 6 33 5 1 1 86 39 0.0 6 38 1 87 59 0.0 6 49 8 1 0 1 88 35 0.0 6 31 4 89 55 0.0 6 49 5 0 0 1 90 55 0.0 6 50 4 1 91 59 0.0 6 56 2 0 1 92 59 0.0 6 57 2 93 92 0.0 6 85 7 94 93 0.0 6 89 2 0 0 2 95 63 0.0 6 63 96 83 0.0 6 78 3 1 0 1 97 66 0.0 6 63 3 98 76 0.0 6 74 2 99 82 0.0 6 78 4 100 69 0.0 6 67 2 101 92 0.0 6 85 6 1 102 119 0.0 6 112 6 0 0 1 103 130 0.0 6 123 6 1 104 148 0.0 6 139 8 1 105 108 0.0 6 104 4 106 82 0.0 6 80 2 107 94 0.0 6 93 0 1 108 113 0.0 6 106 6 0 1 109 99 0.0 6 94 5 110 130 0.0 6 125 4 0 1 111 116 0.0 6 107 9 112 106 0.0 6 104 2 113 115 0.0 6 112 2 0 0 1 114 140 0.0 6 129 9 1 0 0 1 115 116 0.0 6 114 2 116 131 0.0 6 125 6 117 122 0.0 6 119 3 118 133 0.0 6 125 6 0 0 1 0 1 119 127 0.0 6 118 8 0 0 1 120 195 0.0 6 185 10 121 169 0.0 6 155 12 0 2 122 182 0.0 6 176 4 0 1 0 0 1 123 186 0.0 6 179 6 0 0 1 124 228 0.0 6 221 6 1 125 252 0.0 6 245 5 1 0 1 126 205 0.0 6 196 8 0 0 0 1 127 233 0.0 6 224 9 128 251 0.0 6 245 4 0 2 129 216 0.0 6 209 6 1 130 247 0.0 6 235 9 2 0 0 0 1 131 353 0.0 6 339 14 132 373 0.0 6 359 13 1 133 368 0.0 6 359 7 0 1 1 134 512 0.0 6 497 14 0 0 0 1 135 587 0.0 6 567 17 1 0 2 136 337 0.0 6 320 16 1 137 489 0.0 6 476 12 0 0 0 0 1 138 405 0.0 6 394 10 1 139 421 0.0 6 412 7 0 0 1 0 1 140 584 0.0 6 577 7 141 769 0.0 6 754 14 0 0 1 142 843 0.0 6 824 17 0 0 1 1 143 707 0.0 6 687 18 2 144 882 0.0 6 860 20 2 145 797 0.0 6 772 23 2 146 728 0.0 6 714 12 1 0 1 147 621 0.0 6 611 4 2 0 2 2 148 817 0.0 6 804 12 1 149 1079 0.0 6 1057 21 0 0 0 0 1 150 1518 0.0 6 1490 23 1 1 2 1 151 819 0.0 6 807 10 0 0 2 152 1369 0.0 6 1341 27 1 153 993 0.0 6 968 24 0 0 0 1 154 1013 0.0 6 986 25 1 0 1 155 1064 0.0 6 1047 12 2 1 0 1 1 156 892 0.0 6 880 11 0 1 157 1193 0.0 6 1172 20 0 0 0 0 1 158 1167 0.0 6 1142 22 3 159 722 0.0 6 704 14 1 0 0 1 2 160 1107 0.0 6 1089 16 0 0 2 161 881 0.0 6 863 15 0 0 2 1 162 665 0.0 6 653 12 163 886 0.0 6 864 18 1 0 3 164 846 0.0 6 828 15 3 165 852 0.0 6 834 15 1 0 1 0 1 166 1063 0.0 6 1046 15 2 167 735 0.0 6 716 17 1 0 0 0 1 168 1422 0.0 6 1396 24 1 0 1 169 691 0.0 6 678 12 0 0 1 170 797 0.0 6 781 15 0 0 0 1 171 1376 0.0 6 1330 46 172 723 0.0 6 708 11 2 0 1 1 173 772 0.0 6 758 14 174 998 0.0 6 968 29 0 1 175 1400 0.0 6 1364 31 1 1 2 1 176 1013 0.0 6 985 26 0 1 1 177 957 0.0 6 937 19 1 178 958 0.0 6 934 23 1 179 981 0.0 6 951 28 1 1 180 787 0.0 6 763 21 2 1 181 979 0.0 6 956 22 1 182 378 0.0 6 359 18 0 0 1 183 900 0.0 6 869 29 1 0 0 0 1 184 1127 0.0 6 1101 24 0 0 2 185 1288 0.0 6 1255 26 5 0 1 1 186 1760 0.0 6 1698 59 1 0 2 187 1506 0.0 6 1462 41 2 0 1 188 1319 0.0 6 1285 29 1 1 3 189 1443 0.0 6 1408 35 190 1719 0.0 6 1660 57 2 191 1688 0.0 6 1636 49 1 0 2 192 1030 0.0 6 1007 23 193 731 0.0 6 703 24 1 2 0 1 194 1242 0.0 6 1209 30 2 1 195 1645 0.0 6 1583 59 0 1 2 196 3051 0.0 6 2965 81 3 0 1 1 197 904 0.0 6 872 30 0 2 198 1849 0.0 6 1791 53 4 1 199 2802 0.0 6 2727 74 1 200 979 0.0 6 945 32 1 0 1 201 1313 0.0 6 1281 29 2 0 1 202 755 0.0 6 741 14 203 712 0.0 6 693 18 1 204 699 0.0 6 681 16 0 1 1 205 343 0.0 6 336 5 2 206 317 0.0 6 309 8 207 228 0.0 6 221 7 208 405 0.0 6 393 11 1 209 256 0.0 6 250 6 210 275 0.0 6 262 13 211 235 0.0 6 228 7 212 402 0.0 6 391 10 0 1 213 179 0.0 6 169 10 214 669 0.0 6 657 10 2 215 470 0.0 6 457 11 2 216 759 0.0 6 746 13 217 284 0.0 6 270 14 218 243 0.0 6 238 5 219 244 0.0 6 237 7 220 109 0.0 6 108 1 221 257 0.0 6 244 11 2 222 553 0.0 6 538 13 1 0 1 223 633 0.0 6 614 18 0 0 1 224 165 0.0 6 161 4 225 350 0.0 6 340 10 226 169 0.0 6 164 4 0 0 1 227 242 0.0 6 235 6 1 228 157 0.0 6 152 4 1 229 307 0.0 6 304 3 230 256 0.0 6 247 9 231 230 0.0 6 225 5 232 374 0.0 6 367 6 1 233 184 0.0 6 178 6 234 113 0.0 6 111 2 235 44 0.0 6 41 2 0 0 0 0 1 236 17 0.0 6 15 2 237 20 0.0 6 18 2 238 60 0.0 6 59 1 239 36 0.0 6 35 1 240 78 0.0 6 76 2 241 133 0.0 6 130 3 242 161 0.0 6 158 3 243 157 0.0 6 152 5 244 102 0.0 6 99 2 1 245 45 0.0 6 44 1 246 44 0.0 6 42 2 247 17 0.0 6 17 248 41 0.0 6 40 1 249 14 0.0 6 13 1 250 12 0.0 6 11 1 251 32 0.0 6 32 252 3 0.0 6 3 253 2 0.0 6 2 254 1 0.0 6 1 255 1 0.0 6 1 257 1 0.0 6 1 260 1 0.0 6 1 274 1 0.0 6 1 277 5 0.0 6 5 278 56 0.0 6 53 3 279 69 0.0 6 66 3 280 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 597 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 52 2 0.0 6 2 55 3 0.0 6 3 56 1 0.0 6 1 57 1 0.0 6 1 58 1 0.0 6 1 59 1 0.0 6 1 65 1 0.0 6 1 67 1 0.0 6 1 71 2 0.0 6 2 72 9 0.0 6 9 73 9 0.0 6 9 74 15 0.0 6 15 75 8 0.0 6 8 76 12 0.0 6 12 77 2 0.0 6 2 78 2 0.0 6 2 79 4 0.0 6 4 80 4 0.0 6 4 81 5 0.0 6 5 82 4 0.0 6 4 83 2 0.0 6 2 84 2 0.0 6 2 85 4 0.0 6 4 86 3 0.0 6 3 87 5 0.0 6 5 89 5 0.0 6 5 90 5 0.0 6 5 91 3 0.0 6 3 92 8 0.0 6 8 93 1 0.0 6 1 94 4 0.0 6 4 95 9 0.0 6 9 96 1 0.0 6 1 97 4 0.0 6 4 98 8 0.0 6 8 99 4 0.0 6 4 100 2 0.0 6 2 101 3 0.0 6 3 102 4 0.0 6 4 103 4 0.0 6 4 104 4 0.0 6 4 105 2 0.0 6 2 106 3 0.0 6 3 107 3 0.0 6 3 108 6 0.0 6 6 109 1 0.0 6 1 110 16 0.0 6 16 111 5 0.0 6 5 112 1 0.0 6 1 113 5 0.0 6 5 114 6 0.0 6 6 115 5 0.0 6 5 116 3 0.0 6 3 117 6 0.0 6 6 118 3 0.0 6 3 119 3 0.0 6 3 120 5 0.0 6 5 121 2 0.0 6 2 122 3 0.0 6 3 123 5 0.0 6 5 124 5 0.0 6 5 125 10 0.0 6 10 126 2 0.0 6 2 127 5 0.0 6 5 128 4 0.0 6 4 129 3 0.0 6 3 130 3 0.0 6 3 131 4 0.0 6 4 132 2 0.0 6 2 133 4 0.0 6 4 134 4 0.0 6 4 135 5 0.0 6 5 136 6 0.0 6 6 137 6 0.0 6 6 138 2 0.0 6 2 139 5 0.0 6 5 140 2 0.0 6 2 141 2 0.0 6 2 143 8 0.0 6 8 144 12 0.0 6 12 145 4 0.0 6 4 146 1 0.0 6 1 147 4 0.0 6 4 148 9 0.0 6 9 149 4 0.0 6 4 150 1 0.0 6 1 151 8 0.0 6 8 152 6 0.0 6 6 153 4 0.0 6 4 154 2 0.0 6 2 155 4 0.0 6 4 156 2 0.0 6 2 157 4 0.0 6 4 158 7 0.0 6 7 159 3 0.0 6 2 0 0 1 161 4 0.0 6 4 162 4 0.0 6 4 163 11 0.0 6 11 164 4 0.0 6 4 165 7 0.0 6 7 166 5 0.0 6 5 167 5 0.0 6 5 168 6 0.0 6 6 169 7 0.0 6 7 170 6 0.0 6 6 171 2 0.0 6 2 172 7 0.0 6 7 173 4 0.0 6 4 174 3 0.0 6 3 175 8 0.0 6 8 176 5 0.0 6 5 177 5 0.0 6 5 178 6 0.0 6 6 179 3 0.0 6 3 180 3 0.0 6 3 181 3 0.0 6 3 182 5 0.0 6 5 183 2 0.0 6 2 184 5 0.0 6 5 185 1 0.0 6 1 186 3 0.0 6 3 187 3 0.0 6 3 188 2 0.0 6 2 189 9 0.0 6 9 190 1 0.0 6 1 191 3 0.0 6 3 192 3 0.0 6 3 193 1 0.0 6 1 194 3 0.0 6 3 195 1 0.0 6 1 196 2 0.0 6 2 197 1 0.0 6 1 198 3 0.0 6 3 202 1 0.0 6 1 203 2 0.0 6 2 204 2 0.0 6 2 205 1 0.0 6 1 206 5 0.0 6 5 211 1 0.0 6 1 212 2 0.0 6 2 213 4 0.0 6 4 214 1 0.0 6 1 215 1 0.0 6 1 218 2 0.0 6 2 226 1 0.0 6 1 227 1 0.0 6 1 228 2 0.0 6 2 229 1 0.0 6 1 234 1 0.0 6 0 0 1 244 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_04_111_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_04_338_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_04_111_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_04_338_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.82 s (55 µs/read; 1.09 M reads/minute). === Summary === Total read pairs processed: 69,193 Read 1 with adapter: 69,179 (100.0%) Read 2 with adapter: 69,160 (100.0%) == Read fate breakdown == Pairs that were too short: 32 (0.0%) Pairs discarded as untrimmed: 47 (0.1%) Pairs written (passing filters): 69,114 (99.9%) Total basepairs processed: 29,627,992 bp Read 1: 16,671,464 bp Read 2: 12,956,528 bp Total written (filtered): 4,312,169 bp (14.6%) Read 1: 2,274,740 bp Read 2: 2,037,429 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 67627 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1081.1 0 1 4 8 270.3 1 4 4 6 2 16.9 1 1 0 1 7 2 4.2 2 1 0 1 8 1 1.1 2 1 9 3 0.3 2 2 1 15 2 0.0 4 2 16 4 0.0 4 4 17 1 0.0 5 1 19 3 0.0 5 2 1 20 90 0.0 6 89 1 21 45 0.0 6 45 22 48 0.0 6 48 23 30 0.0 6 30 24 26 0.0 6 26 25 22 0.0 6 21 1 26 22 0.0 6 21 0 0 0 0 0 1 27 13 0.0 6 13 28 13 0.0 6 13 29 4 0.0 6 4 30 6 0.0 6 6 31 14 0.0 6 13 1 32 8 0.0 6 8 33 1 0.0 6 1 34 8 0.0 6 7 0 0 0 0 1 35 5 0.0 6 5 36 6 0.0 6 5 1 37 4 0.0 6 4 38 8 0.0 6 8 39 10 0.0 6 9 1 40 9 0.0 6 9 41 10 0.0 6 10 42 6 0.0 6 6 43 7 0.0 6 6 1 44 5 0.0 6 4 1 45 8 0.0 6 8 46 4 0.0 6 4 47 8 0.0 6 6 2 48 7 0.0 6 7 49 10 0.0 6 10 50 12 0.0 6 11 1 51 10 0.0 6 10 52 7 0.0 6 7 53 7 0.0 6 7 54 6 0.0 6 6 55 5 0.0 6 5 56 4 0.0 6 4 57 4 0.0 6 4 58 4 0.0 6 4 59 8 0.0 6 8 60 6 0.0 6 5 0 0 1 61 5 0.0 6 4 0 0 1 62 8 0.0 6 7 1 63 8 0.0 6 8 64 10 0.0 6 10 65 10 0.0 6 10 66 18 0.0 6 16 2 67 8 0.0 6 8 68 3 0.0 6 3 69 11 0.0 6 10 1 70 10 0.0 6 10 71 11 0.0 6 11 72 8 0.0 6 8 73 9 0.0 6 8 1 74 13 0.0 6 13 75 13 0.0 6 12 1 76 12 0.0 6 11 1 77 4 0.0 6 4 78 9 0.0 6 9 79 9 0.0 6 9 80 9 0.0 6 9 81 8 0.0 6 8 82 10 0.0 6 10 83 14 0.0 6 12 2 84 15 0.0 6 14 1 85 7 0.0 6 7 86 6 0.0 6 6 87 9 0.0 6 9 88 8 0.0 6 8 89 7 0.0 6 6 1 90 16 0.0 6 16 91 8 0.0 6 8 92 19 0.0 6 18 1 93 5 0.0 6 5 94 14 0.0 6 13 1 95 10 0.0 6 10 96 14 0.0 6 13 0 0 0 0 1 97 11 0.0 6 11 98 7 0.0 6 7 99 9 0.0 6 9 100 14 0.0 6 13 1 101 15 0.0 6 15 102 16 0.0 6 15 0 0 0 0 1 103 14 0.0 6 14 104 17 0.0 6 17 105 14 0.0 6 14 106 16 0.0 6 16 107 9 0.0 6 9 108 19 0.0 6 18 0 0 1 109 14 0.0 6 14 110 20 0.0 6 20 111 24 0.0 6 24 112 24 0.0 6 23 1 113 22 0.0 6 22 114 19 0.0 6 19 115 16 0.0 6 14 2 116 18 0.0 6 17 0 0 1 117 23 0.0 6 22 1 118 19 0.0 6 19 119 18 0.0 6 16 2 120 25 0.0 6 25 121 29 0.0 6 27 2 122 20 0.0 6 20 123 33 0.0 6 33 124 28 0.0 6 27 1 125 31 0.0 6 31 126 32 0.0 6 31 1 127 37 0.0 6 37 128 21 0.0 6 21 129 34 0.0 6 34 130 35 0.0 6 34 1 131 43 0.0 6 41 2 132 46 0.0 6 44 2 133 32 0.0 6 30 1 0 1 134 45 0.0 6 44 1 135 27 0.0 6 27 136 43 0.0 6 43 137 55 0.0 6 54 1 138 61 0.0 6 60 1 139 66 0.0 6 65 1 140 166 0.0 6 163 3 141 282 0.0 6 281 1 142 325 0.0 6 325 143 263 0.0 6 263 144 266 0.0 6 265 1 145 333 0.0 6 332 1 146 253 0.0 6 253 147 277 0.0 6 277 148 355 0.0 6 353 2 149 265 0.0 6 264 1 150 231 0.0 6 231 151 224 0.0 6 220 3 0 0 0 1 152 230 0.0 6 230 153 227 0.0 6 227 154 245 0.0 6 245 155 228 0.0 6 226 1 1 156 217 0.0 6 217 157 239 0.0 6 238 1 158 238 0.0 6 237 1 159 289 0.0 6 287 2 160 306 0.0 6 304 2 161 255 0.0 6 252 3 162 318 0.0 6 318 163 322 0.0 6 321 1 164 271 0.0 6 267 4 165 248 0.0 6 244 3 0 0 0 0 1 166 281 0.0 6 280 1 167 293 0.0 6 292 1 168 288 0.0 6 286 2 169 351 0.0 6 348 2 0 0 0 0 1 170 297 0.0 6 295 1 0 0 0 0 1 171 298 0.0 6 294 2 0 2 172 297 0.0 6 296 1 173 379 0.0 6 377 2 174 291 0.0 6 288 2 0 0 0 0 1 175 323 0.0 6 320 3 176 347 0.0 6 345 2 177 306 0.0 6 305 1 178 554 0.0 6 552 2 179 362 0.0 6 361 1 180 357 0.0 6 355 2 181 366 0.0 6 366 182 346 0.0 6 338 8 183 399 0.0 6 397 2 184 402 0.0 6 398 4 185 360 0.0 6 358 2 186 380 0.0 6 377 3 187 493 0.0 6 487 5 1 188 358 0.0 6 358 189 369 0.0 6 364 4 0 1 190 437 0.0 6 435 1 0 1 191 469 0.0 6 466 2 0 0 1 192 554 0.0 6 550 4 193 561 0.0 6 558 3 194 575 0.0 6 569 6 195 669 0.0 6 664 5 196 907 0.0 6 902 4 0 0 1 197 796 0.0 6 790 5 0 1 198 916 0.0 6 910 5 1 199 706 0.0 6 704 2 200 642 0.0 6 637 4 0 0 0 1 201 669 0.0 6 663 6 202 713 0.0 6 707 5 0 0 0 1 203 599 0.0 6 594 5 204 623 0.0 6 617 6 205 791 0.0 6 790 1 206 960 0.0 6 955 4 0 0 0 1 207 728 0.0 6 725 3 208 812 0.0 6 806 6 209 649 0.0 6 646 3 210 714 0.0 6 713 1 211 836 0.0 6 829 6 0 0 1 212 854 0.0 6 846 5 1 0 1 0 1 213 862 0.0 6 858 3 0 0 0 1 214 1025 0.0 6 1018 7 215 1183 0.0 6 1173 9 0 0 0 1 216 906 0.0 6 896 10 217 898 0.0 6 887 9 0 0 1 1 218 958 0.0 6 950 7 0 0 0 1 219 903 0.0 6 900 3 220 688 0.0 6 686 2 221 687 0.0 6 683 4 222 727 0.0 6 721 4 1 0 0 0 1 223 766 0.0 6 764 1 0 0 0 1 224 774 0.0 6 769 3 1 0 1 225 823 0.0 6 816 5 2 226 852 0.0 6 846 6 227 648 0.0 6 643 4 0 0 0 1 228 678 0.0 6 671 6 1 229 666 0.0 6 661 5 230 681 0.0 6 674 6 0 0 1 231 691 0.0 6 686 3 1 1 232 705 0.0 6 703 0 1 0 0 1 233 678 0.0 6 677 0 0 0 0 0 1 234 644 0.0 6 639 2 0 1 0 0 2 235 592 0.0 6 587 4 0 0 1 236 567 0.0 6 563 3 0 0 0 1 237 546 0.0 6 539 7 238 618 0.0 6 614 4 239 510 0.0 6 508 1 1 240 555 0.0 6 551 4 241 568 0.0 6 566 2 242 492 0.0 6 491 1 243 498 0.0 6 493 4 1 244 507 0.0 6 504 3 245 414 0.0 6 409 3 0 1 0 0 1 246 358 0.0 6 357 1 247 342 0.0 6 340 2 248 336 0.0 6 335 1 249 308 0.0 6 308 250 338 0.0 6 338 251 307 0.0 6 307 252 281 0.0 6 279 1 0 0 0 1 253 323 0.0 6 322 1 254 306 0.0 6 305 1 255 279 0.0 6 278 1 256 283 0.0 6 280 2 1 257 265 0.0 6 262 3 258 265 0.0 6 264 0 1 259 291 0.0 6 291 260 229 0.0 6 228 1 261 208 0.0 6 208 262 216 0.0 6 215 1 263 186 0.0 6 186 264 162 0.0 6 162 265 181 0.0 6 180 1 266 182 0.0 6 180 2 267 187 0.0 6 187 268 184 0.0 6 182 0 1 0 0 1 269 189 0.0 6 188 1 270 148 0.0 6 145 3 271 140 0.0 6 138 1 1 272 108 0.0 6 108 273 174 0.0 6 174 274 194 0.0 6 192 2 275 212 0.0 6 209 1 0 0 1 0 1 276 265 0.0 6 261 4 277 367 0.0 6 366 1 278 1078 0.0 6 1070 7 1 279 1827 0.0 6 1818 8 0 1 280 9 0.0 6 9 281 3 0.0 6 2 0 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1552 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 2 0.0 6 0 2 63 83 0.0 6 81 2 64 1 0.0 6 1 68 1 0.0 6 0 0 0 0 0 0 1 71 1 0.0 6 1 72 5 0.0 6 5 73 9 0.0 6 9 74 11 0.0 6 11 75 7 0.0 6 7 76 9 0.0 6 9 77 10 0.0 6 10 78 9 0.0 6 8 1 79 5 0.0 6 5 80 12 0.0 6 12 81 4 0.0 6 4 82 5 0.0 6 5 83 8 0.0 6 8 84 6 0.0 6 6 85 8 0.0 6 8 86 10 0.0 6 10 87 7 0.0 6 7 88 5 0.0 6 5 89 2 0.0 6 2 90 12 0.0 6 12 91 11 0.0 6 11 92 5 0.0 6 5 93 7 0.0 6 7 94 6 0.0 6 6 95 6 0.0 6 6 96 6 0.0 6 6 97 3 0.0 6 3 98 13 0.0 6 13 99 6 0.0 6 6 100 7 0.0 6 7 101 6 0.0 6 6 102 5 0.0 6 4 1 103 6 0.0 6 6 104 5 0.0 6 5 105 7 0.0 6 7 106 3 0.0 6 3 107 6 0.0 6 6 108 4 0.0 6 4 109 13 0.0 6 13 110 18 0.0 6 17 0 1 111 5 0.0 6 5 112 8 0.0 6 8 113 9 0.0 6 9 114 5 0.0 6 5 115 8 0.0 6 8 116 4 0.0 6 4 117 5 0.0 6 5 118 6 0.0 6 6 119 11 0.0 6 11 120 6 0.0 6 6 121 4 0.0 6 4 122 12 0.0 6 12 123 8 0.0 6 8 124 4 0.0 6 4 125 3 0.0 6 3 126 3 0.0 6 3 127 9 0.0 6 9 128 6 0.0 6 6 129 5 0.0 6 5 130 8 0.0 6 8 131 4 0.0 6 4 132 7 0.0 6 7 133 8 0.0 6 8 134 15 0.0 6 15 135 1 0.0 6 1 136 8 0.0 6 8 137 6 0.0 6 6 138 8 0.0 6 8 139 6 0.0 6 6 140 3 0.0 6 3 141 5 0.0 6 5 142 5 0.0 6 5 143 10 0.0 6 10 144 10 0.0 6 10 145 6 0.0 6 6 146 14 0.0 6 14 147 7 0.0 6 7 148 9 0.0 6 9 149 13 0.0 6 13 150 6 0.0 6 6 151 8 0.0 6 8 152 8 0.0 6 8 153 4 0.0 6 4 154 6 0.0 6 6 155 4 0.0 6 4 156 6 0.0 6 6 157 8 0.0 6 8 158 13 0.0 6 13 159 4 0.0 6 4 160 8 0.0 6 8 161 9 0.0 6 9 162 9 0.0 6 9 163 7 0.0 6 7 164 12 0.0 6 12 165 3 0.0 6 3 166 5 0.0 6 5 167 14 0.0 6 14 168 11 0.0 6 11 169 14 0.0 6 14 170 5 0.0 6 5 171 8 0.0 6 8 172 8 0.0 6 8 173 10 0.0 6 10 174 7 0.0 6 7 175 8 0.0 6 8 176 11 0.0 6 11 177 14 0.0 6 14 178 16 0.0 6 16 179 8 0.0 6 8 180 6 0.0 6 6 181 6 0.0 6 6 182 6 0.0 6 6 183 10 0.0 6 10 184 4 0.0 6 4 185 13 0.0 6 13 186 14 0.0 6 14 187 10 0.0 6 10 188 13 0.0 6 13 189 14 0.0 6 14 190 15 0.0 6 15 191 12 0.0 6 12 192 8 0.0 6 8 193 8 0.0 6 8 194 10 0.0 6 10 195 9 0.0 6 9 196 9 0.0 6 9 197 9 0.0 6 9 198 6 0.0 6 6 199 13 0.0 6 13 200 12 0.0 6 12 201 6 0.0 6 6 202 6 0.0 6 6 203 16 0.0 6 16 204 6 0.0 6 6 205 8 0.0 6 8 206 12 0.0 6 12 207 8 0.0 6 8 208 5 0.0 6 5 209 12 0.0 6 12 210 6 0.0 6 6 211 10 0.0 6 10 212 12 0.0 6 12 213 9 0.0 6 9 214 7 0.0 6 7 215 11 0.0 6 11 216 14 0.0 6 14 217 7 0.0 6 7 218 5 0.0 6 5 219 7 0.0 6 7 220 5 0.0 6 5 221 6 0.0 6 6 222 7 0.0 6 7 223 8 0.0 6 8 224 11 0.0 6 11 225 7 0.0 6 7 226 6 0.0 6 6 227 4 0.0 6 4 228 14 0.0 6 14 229 5 0.0 6 5 230 7 0.0 6 7 231 3 0.0 6 3 232 4 0.0 6 4 233 5 0.0 6 5 234 7 0.0 6 7 235 9 0.0 6 9 236 3 0.0 6 3 237 5 0.0 6 5 238 5 0.0 6 5 239 6 0.0 6 6 240 3 0.0 6 3 241 3 0.0 6 3 242 7 0.0 6 7 243 2 0.0 6 2 244 8 0.0 6 8 245 6 0.0 6 6 246 8 0.0 6 8 247 3 0.0 6 3 248 7 0.0 6 7 249 5 0.0 6 5 250 5 0.0 6 5 251 4 0.0 6 4 252 6 0.0 6 6 253 5 0.0 6 5 254 5 0.0 6 5 255 5 0.0 6 5 257 3 0.0 6 3 258 3 0.0 6 3 259 7 0.0 6 7 260 1 0.0 6 1 261 2 0.0 6 2 262 3 0.0 6 3 263 1 0.0 6 1 264 2 0.0 6 2 265 1 0.0 6 1 266 2 0.0 6 2 267 3 0.0 6 3 268 2 0.0 6 2 269 3 0.0 6 3 270 4 0.0 6 4 271 2 0.0 6 2 272 1 0.0 6 1 273 1 0.0 6 1 274 2 0.0 6 2 275 1 0.0 6 1 276 2 0.0 6 2 277 1 0.0 6 1 278 2 0.0 6 2 280 1 0.0 6 1 283 1 0.0 6 1 285 1 0.0 6 1 286 2 0.0 6 2 287 1 0.0 6 1 289 2 0.0 6 2 292 1 0.0 6 1 295 1 0.0 6 1 296 2 0.0 6 2 297 3 0.0 6 3 298 1 0.0 6 1 311 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 68058 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1081.1 0 1 8 1 1.1 2 1 10 1 0.1 3 1 11 2 0.0 3 1 0 1 12 4 0.0 3 4 13 4 0.0 3 3 1 14 12 0.0 4 11 1 15 2 0.0 4 2 16 4 0.0 4 4 17 27 0.0 5 23 3 1 18 4 0.0 5 2 2 19 46 0.0 5 40 4 2 20 99 0.0 6 94 5 21 55 0.0 6 53 2 22 47 0.0 6 45 2 23 30 0.0 6 25 4 1 24 19 0.0 6 18 1 25 21 0.0 6 19 1 0 0 1 26 22 0.0 6 21 1 27 18 0.0 6 17 0 0 0 1 28 23 0.0 6 22 1 29 20 0.0 6 20 30 12 0.0 6 11 1 31 18 0.0 6 17 1 32 20 0.0 6 16 4 33 18 0.0 6 16 2 34 17 0.0 6 16 1 35 12 0.0 6 12 36 21 0.0 6 19 2 37 16 0.0 6 13 2 0 0 0 1 38 15 0.0 6 13 1 0 0 1 39 10 0.0 6 8 2 40 10 0.0 6 9 1 41 22 0.0 6 18 4 42 12 0.0 6 9 1 0 2 43 18 0.0 6 15 1 2 44 17 0.0 6 16 1 45 19 0.0 6 17 2 46 10 0.0 6 8 1 1 47 14 0.0 6 13 1 48 20 0.0 6 19 1 49 21 0.0 6 19 1 0 0 0 1 50 13 0.0 6 11 2 51 28 0.0 6 22 4 2 52 28 0.0 6 28 53 21 0.0 6 15 6 54 25 0.0 6 23 2 55 17 0.0 6 16 1 56 20 0.0 6 19 1 57 22 0.0 6 21 1 58 24 0.0 6 23 1 59 25 0.0 6 23 2 60 31 0.0 6 28 2 0 0 1 61 39 0.0 6 36 2 0 0 0 1 62 23 0.0 6 19 2 1 1 63 35 0.0 6 29 4 1 0 1 64 23 0.0 6 22 1 65 26 0.0 6 24 2 66 31 0.0 6 28 0 0 1 2 67 40 0.0 6 40 68 37 0.0 6 34 2 0 1 69 61 0.0 6 57 4 70 145 0.0 6 141 3 1 71 50 0.0 6 41 6 2 0 1 72 71 0.0 6 64 6 0 0 1 73 49 0.0 6 45 4 74 51 0.0 6 49 1 1 75 41 0.0 6 39 1 1 76 37 0.0 6 35 1 0 0 0 1 77 42 0.0 6 39 1 0 1 1 78 47 0.0 6 44 3 79 41 0.0 6 35 4 0 0 0 1 1 80 33 0.0 6 27 5 1 81 45 0.0 6 40 4 1 82 68 0.0 6 62 4 0 0 0 1 1 83 70 0.0 6 67 2 1 84 52 0.0 6 50 1 1 85 60 0.0 6 58 1 0 0 0 1 86 80 0.0 6 77 0 1 0 0 1 1 87 47 0.0 6 46 1 88 58 0.0 6 53 3 2 89 49 0.0 6 45 3 0 0 1 90 52 0.0 6 50 2 91 70 0.0 6 63 5 0 2 92 65 0.0 6 60 5 93 74 0.0 6 72 1 0 0 0 0 1 94 73 0.0 6 70 2 1 95 78 0.0 6 73 4 0 0 0 1 96 89 0.0 6 86 3 97 75 0.0 6 74 1 98 93 0.0 6 87 6 99 92 0.0 6 88 4 100 86 0.0 6 81 5 101 78 0.0 6 76 1 0 1 102 96 0.0 6 94 2 103 87 0.0 6 82 5 104 104 0.0 6 99 5 105 93 0.0 6 87 4 1 0 0 0 1 106 139 0.0 6 137 2 107 209 0.0 6 200 6 1 0 0 2 108 124 0.0 6 119 4 0 0 1 109 102 0.0 6 101 1 110 127 0.0 6 124 1 1 0 1 111 120 0.0 6 118 1 0 0 1 112 117 0.0 6 116 1 113 159 0.0 6 155 3 0 0 0 1 114 156 0.0 6 153 3 115 158 0.0 6 155 1 1 0 0 1 116 163 0.0 6 157 5 0 1 117 147 0.0 6 139 5 1 2 118 166 0.0 6 163 2 1 119 159 0.0 6 154 4 1 120 198 0.0 6 194 4 121 241 0.0 6 233 7 1 122 208 0.0 6 197 11 123 178 0.0 6 173 3 0 0 1 0 1 124 169 0.0 6 166 2 0 0 0 0 1 125 210 0.0 6 203 4 1 1 0 1 126 295 0.0 6 290 5 127 274 0.0 6 264 8 0 1 0 0 1 128 334 0.0 6 326 6 1 0 1 129 288 0.0 6 280 5 2 0 0 0 1 130 348 0.0 6 340 8 131 421 0.0 6 409 10 1 0 0 0 1 132 375 0.0 6 362 10 2 0 1 133 425 0.0 6 416 7 1 1 134 429 0.0 6 418 8 1 1 0 1 135 432 0.0 6 425 6 0 0 0 0 1 136 468 0.0 6 456 10 1 1 137 516 0.0 6 503 12 1 138 513 0.0 6 501 10 0 1 0 1 139 531 0.0 6 521 9 0 1 140 608 0.0 6 597 6 0 1 0 1 3 141 670 0.0 6 660 8 2 142 786 0.0 6 777 8 0 1 143 752 0.0 6 740 10 1 0 1 144 743 0.0 6 734 8 1 145 898 0.0 6 887 10 1 146 824 0.0 6 810 12 0 0 2 147 919 0.0 6 905 14 148 994 0.0 6 977 16 0 0 0 0 1 149 988 0.0 6 972 11 1 0 1 1 2 150 928 0.0 6 915 11 1 0 1 151 1119 0.0 6 1105 9 2 1 1 1 152 1223 0.0 6 1197 20 1 1 1 1 2 153 1508 0.0 6 1485 21 0 0 0 1 1 154 1176 0.0 6 1152 24 155 1522 0.0 6 1497 23 1 0 1 156 1309 0.0 6 1290 15 2 0 0 1 1 157 1176 0.0 6 1160 11 2 1 1 1 158 1286 0.0 6 1273 11 0 0 1 1 159 1075 0.0 6 1056 16 1 0 1 0 1 160 1256 0.0 6 1242 9 2 0 0 3 161 1197 0.0 6 1179 16 0 0 0 2 162 1072 0.0 6 1055 12 1 1 0 2 1 163 1095 0.0 6 1083 11 0 1 164 989 0.0 6 971 16 0 1 1 165 908 0.0 6 892 10 0 2 0 2 2 166 990 0.0 6 980 7 1 2 167 940 0.0 6 927 12 0 0 0 1 168 891 0.0 6 874 15 2 169 1165 0.0 6 1154 10 0 0 0 0 1 170 882 0.0 6 876 5 0 0 0 1 171 1031 0.0 6 1024 6 0 0 0 0 1 172 950 0.0 6 936 13 1 173 924 0.0 6 914 8 1 1 174 1006 0.0 6 991 11 1 1 0 1 1 175 1028 0.0 6 1014 10 1 0 1 0 2 176 1025 0.0 6 1014 7 1 1 1 0 1 177 859 0.0 6 847 10 1 0 0 0 1 178 850 0.0 6 840 9 0 0 0 1 179 937 0.0 6 923 13 1 180 706 0.0 6 702 4 181 747 0.0 6 741 5 0 0 1 182 557 0.0 6 551 6 183 553 0.0 6 548 3 1 0 0 0 1 184 545 0.0 6 534 9 1 0 1 185 520 0.0 6 515 5 186 820 0.0 6 810 10 187 1038 0.0 6 1029 8 1 188 771 0.0 6 761 9 1 189 575 0.0 6 567 6 0 0 2 190 559 0.0 6 554 3 0 0 1 1 191 522 0.0 6 515 5 0 1 0 0 1 192 518 0.0 6 511 7 193 468 0.0 6 464 3 0 0 1 194 498 0.0 6 494 4 195 332 0.0 6 330 2 196 400 0.0 6 399 1 197 299 0.0 6 293 5 1 198 274 0.0 6 272 1 0 0 0 1 199 402 0.0 6 392 7 0 0 0 2 1 200 256 0.0 6 254 2 201 282 0.0 6 280 2 202 216 0.0 6 213 3 203 224 0.0 6 223 1 204 184 0.0 6 182 2 205 208 0.0 6 206 2 206 183 0.0 6 180 2 0 0 1 207 167 0.0 6 165 2 208 154 0.0 6 154 209 152 0.0 6 151 0 0 0 0 1 210 136 0.0 6 136 211 142 0.0 6 139 3 212 135 0.0 6 134 1 213 90 0.0 6 88 2 214 109 0.0 6 107 2 215 109 0.0 6 109 216 100 0.0 6 100 217 98 0.0 6 98 218 70 0.0 6 69 1 219 76 0.0 6 73 3 220 60 0.0 6 59 0 0 1 221 72 0.0 6 70 1 0 1 222 41 0.0 6 40 0 0 0 0 0 1 223 34 0.0 6 34 224 55 0.0 6 54 1 225 47 0.0 6 45 1 0 0 0 0 1 226 33 0.0 6 32 1 227 26 0.0 6 25 1 228 17 0.0 6 17 229 32 0.0 6 32 230 27 0.0 6 27 231 21 0.0 6 19 1 0 0 0 1 232 16 0.0 6 16 233 14 0.0 6 14 234 14 0.0 6 14 235 8 0.0 6 8 236 5 0.0 6 5 237 7 0.0 6 7 238 5 0.0 6 5 239 4 0.0 6 4 240 3 0.0 6 3 241 2 0.0 6 2 242 6 0.0 6 6 243 3 0.0 6 3 244 5 0.0 6 4 1 245 2 0.0 6 2 246 1 0.0 6 1 247 3 0.0 6 3 248 1 0.0 6 1 251 2 0.0 6 2 254 1 0.0 6 1 255 1 0.0 6 1 278 1 0.0 6 1 279 1 0.0 6 1 280 1 0.0 6 1 281 17 0.0 6 17 282 82 0.0 6 79 3 283 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1102 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 16 1 0.0 4 0 0 0 0 1 50 1 0.0 6 1 59 1 0.0 6 1 60 1 0.0 6 1 62 2 0.0 6 2 65 1 0.0 6 1 66 1 0.0 6 1 67 1 0.0 6 1 68 15 0.0 6 15 69 1 0.0 6 1 70 12 0.0 6 12 71 18 0.0 6 18 72 6 0.0 6 6 73 9 0.0 6 9 74 12 0.0 6 12 75 10 0.0 6 10 76 10 0.0 6 10 77 7 0.0 6 7 78 9 0.0 6 9 79 5 0.0 6 5 80 3 0.0 6 3 81 7 0.0 6 7 82 8 0.0 6 8 83 7 0.0 6 7 84 3 0.0 6 3 85 8 0.0 6 8 86 3 0.0 6 3 87 9 0.0 6 9 88 7 0.0 6 7 89 5 0.0 6 5 90 6 0.0 6 6 91 8 0.0 6 7 0 0 0 0 1 92 12 0.0 6 12 93 7 0.0 6 7 94 4 0.0 6 4 95 6 0.0 6 6 96 9 0.0 6 9 97 12 0.0 6 11 1 98 10 0.0 6 10 99 13 0.0 6 13 100 6 0.0 6 6 101 4 0.0 6 4 102 6 0.0 6 6 103 5 0.0 6 5 104 11 0.0 6 11 105 5 0.0 6 5 106 7 0.0 6 6 1 107 19 0.0 6 19 108 6 0.0 6 6 109 10 0.0 6 10 110 5 0.0 6 5 111 7 0.0 6 7 112 7 0.0 6 7 113 7 0.0 6 7 114 7 0.0 6 7 115 10 0.0 6 10 116 7 0.0 6 7 117 10 0.0 6 10 118 3 0.0 6 3 119 7 0.0 6 7 120 12 0.0 6 11 1 121 8 0.0 6 8 122 4 0.0 6 4 123 6 0.0 6 6 124 10 0.0 6 10 125 11 0.0 6 11 126 4 0.0 6 4 127 6 0.0 6 6 128 14 0.0 6 14 129 4 0.0 6 4 130 3 0.0 6 3 131 13 0.0 6 13 132 3 0.0 6 3 133 9 0.0 6 8 0 0 0 1 134 8 0.0 6 8 135 9 0.0 6 9 136 13 0.0 6 12 0 0 1 137 6 0.0 6 6 138 9 0.0 6 9 139 6 0.0 6 6 140 12 0.0 6 12 141 15 0.0 6 15 142 4 0.0 6 4 143 8 0.0 6 8 144 7 0.0 6 7 145 5 0.0 6 5 146 10 0.0 6 10 147 11 0.0 6 11 148 6 0.0 6 6 149 11 0.0 6 11 150 7 0.0 6 7 151 5 0.0 6 5 152 5 0.0 6 5 153 6 0.0 6 6 154 9 0.0 6 9 155 9 0.0 6 9 156 12 0.0 6 12 157 5 0.0 6 5 158 16 0.0 6 16 159 12 0.0 6 12 160 4 0.0 6 4 161 14 0.0 6 14 162 13 0.0 6 13 163 8 0.0 6 8 164 5 0.0 6 5 165 7 0.0 6 7 166 10 0.0 6 10 167 10 0.0 6 10 168 5 0.0 6 5 169 6 0.0 6 6 170 8 0.0 6 8 171 5 0.0 6 5 172 6 0.0 6 6 173 9 0.0 6 9 174 8 0.0 6 8 175 10 0.0 6 10 176 11 0.0 6 11 177 7 0.0 6 7 178 8 0.0 6 8 179 9 0.0 6 9 180 7 0.0 6 7 181 8 0.0 6 8 182 4 0.0 6 4 183 7 0.0 6 7 184 4 0.0 6 4 185 6 0.0 6 6 186 11 0.0 6 11 187 7 0.0 6 7 188 4 0.0 6 4 189 4 0.0 6 4 190 2 0.0 6 2 192 8 0.0 6 8 193 3 0.0 6 3 194 3 0.0 6 3 195 9 0.0 6 9 196 4 0.0 6 4 197 7 0.0 6 7 198 6 0.0 6 5 1 199 1 0.0 6 1 200 4 0.0 6 4 201 6 0.0 6 6 202 6 0.0 6 6 203 2 0.0 6 2 204 5 0.0 6 5 205 4 0.0 6 4 206 3 0.0 6 3 207 3 0.0 6 3 208 3 0.0 6 3 209 3 0.0 6 3 210 3 0.0 6 3 211 5 0.0 6 5 212 4 0.0 6 4 213 5 0.0 6 5 214 3 0.0 6 3 215 3 0.0 6 3 216 2 0.0 6 2 218 4 0.0 6 4 221 2 0.0 6 2 223 1 0.0 6 1 227 1 0.0 6 1 228 1 0.0 6 1 229 1 0.0 6 1 231 1 0.0 6 1 232 1 0.0 6 1 235 1 0.0 6 1 237 1 0.0 6 1 241 1 0.0 6 1 243 1 0.0 6 1 246 1 0.0 6 1 248 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_05_112_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_05_339_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_05_112_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_05_339_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.31 s (32 µs/read; 1.85 M reads/minute). === Summary === Total read pairs processed: 71,204 Read 1 with adapter: 71,195 (100.0%) Read 2 with adapter: 71,177 (100.0%) == Read fate breakdown == Pairs that were too short: 13 (0.0%) Pairs discarded as untrimmed: 36 (0.1%) Pairs written (passing filters): 71,155 (99.9%) Total basepairs processed: 31,956,076 bp Read 1: 17,948,017 bp Read 2: 14,008,059 bp Total written (filtered): 4,479,154 bp (14.0%) Read 1: 2,320,584 bp Read 2: 2,158,570 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 70299 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1112.6 0 1 6 1 17.4 1 0 0 1 7 2 4.3 2 0 1 1 9 1 0.3 2 1 11 1 0.0 3 1 15 1 0.0 4 0 1 17 5 0.0 5 5 18 1 0.0 5 1 20 75 0.0 6 74 1 21 43 0.0 6 43 22 34 0.0 6 32 2 23 24 0.0 6 24 24 25 0.0 6 24 1 25 15 0.0 6 15 26 23 0.0 6 21 2 27 6 0.0 6 6 28 10 0.0 6 9 1 29 5 0.0 6 5 30 7 0.0 6 7 31 2 0.0 6 1 0 1 32 7 0.0 6 7 33 7 0.0 6 6 1 34 2 0.0 6 2 35 7 0.0 6 7 36 5 0.0 6 4 0 0 0 1 37 3 0.0 6 2 1 38 5 0.0 6 4 1 39 6 0.0 6 6 40 6 0.0 6 6 41 4 0.0 6 4 42 7 0.0 6 5 2 43 3 0.0 6 2 1 44 8 0.0 6 8 45 8 0.0 6 7 1 46 5 0.0 6 4 1 47 5 0.0 6 5 48 5 0.0 6 4 1 49 2 0.0 6 2 50 7 0.0 6 7 51 1 0.0 6 1 52 5 0.0 6 5 53 2 0.0 6 2 54 9 0.0 6 9 55 4 0.0 6 2 2 56 5 0.0 6 5 57 5 0.0 6 5 58 8 0.0 6 7 1 59 3 0.0 6 2 1 60 1 0.0 6 1 61 6 0.0 6 6 62 2 0.0 6 1 1 63 8 0.0 6 8 64 12 0.0 6 10 1 0 0 0 0 1 65 12 0.0 6 12 66 6 0.0 6 6 67 5 0.0 6 5 68 5 0.0 6 5 69 8 0.0 6 7 1 70 5 0.0 6 5 71 6 0.0 6 6 72 3 0.0 6 3 73 3 0.0 6 3 74 16 0.0 6 16 75 12 0.0 6 12 76 5 0.0 6 5 77 7 0.0 6 7 78 4 0.0 6 4 79 1 0.0 6 1 80 5 0.0 6 4 1 81 7 0.0 6 7 82 6 0.0 6 6 83 10 0.0 6 10 84 10 0.0 6 10 85 3 0.0 6 3 86 13 0.0 6 13 87 9 0.0 6 9 88 6 0.0 6 5 0 0 1 89 13 0.0 6 12 1 90 10 0.0 6 9 1 91 5 0.0 6 4 1 92 8 0.0 6 7 1 93 7 0.0 6 5 2 94 3 0.0 6 3 95 7 0.0 6 7 96 6 0.0 6 5 1 97 7 0.0 6 5 2 98 9 0.0 6 8 1 99 6 0.0 6 6 100 10 0.0 6 10 101 10 0.0 6 10 102 12 0.0 6 12 103 14 0.0 6 13 1 104 10 0.0 6 10 105 22 0.0 6 22 106 16 0.0 6 16 107 11 0.0 6 10 0 0 0 0 1 108 19 0.0 6 18 1 109 12 0.0 6 12 110 12 0.0 6 11 0 0 0 1 111 19 0.0 6 19 112 25 0.0 6 24 1 113 20 0.0 6 20 114 28 0.0 6 27 1 115 19 0.0 6 18 1 116 20 0.0 6 20 117 17 0.0 6 17 118 29 0.0 6 27 2 119 18 0.0 6 18 120 19 0.0 6 16 2 1 121 23 0.0 6 22 1 122 22 0.0 6 22 123 16 0.0 6 16 124 24 0.0 6 24 125 37 0.0 6 36 1 126 15 0.0 6 15 127 21 0.0 6 21 128 25 0.0 6 25 129 25 0.0 6 24 0 1 130 22 0.0 6 22 131 24 0.0 6 24 132 27 0.0 6 27 133 15 0.0 6 15 134 41 0.0 6 41 135 28 0.0 6 26 2 136 29 0.0 6 29 137 50 0.0 6 48 2 138 56 0.0 6 54 1 0 0 0 1 139 60 0.0 6 58 2 140 88 0.0 6 87 0 0 0 0 0 1 141 228 0.0 6 226 2 142 313 0.0 6 313 143 426 0.0 6 424 0 0 2 144 341 0.0 6 340 1 145 354 0.0 6 352 1 1 146 518 0.0 6 515 2 0 0 0 0 1 147 387 0.0 6 386 1 148 468 0.0 6 467 1 149 453 0.0 6 450 3 150 415 0.0 6 413 2 151 531 0.0 6 530 0 0 0 1 152 357 0.0 6 356 0 0 0 1 153 262 0.0 6 261 1 154 277 0.0 6 274 3 155 258 0.0 6 257 0 1 156 233 0.0 6 233 157 221 0.0 6 221 158 282 0.0 6 280 2 159 246 0.0 6 245 1 160 300 0.0 6 296 3 0 1 161 354 0.0 6 353 1 162 282 0.0 6 281 1 163 239 0.0 6 238 1 164 312 0.0 6 311 1 165 228 0.0 6 228 166 210 0.0 6 210 167 237 0.0 6 234 3 168 223 0.0 6 221 2 169 249 0.0 6 247 2 170 242 0.0 6 241 0 0 1 171 237 0.0 6 236 1 172 249 0.0 6 249 173 254 0.0 6 254 174 276 0.0 6 274 2 175 254 0.0 6 252 1 0 1 176 264 0.0 6 259 4 0 0 0 0 1 177 256 0.0 6 255 1 178 245 0.0 6 242 3 179 526 0.0 6 525 1 180 256 0.0 6 251 5 181 248 0.0 6 248 182 275 0.0 6 271 3 0 0 1 183 271 0.0 6 269 2 184 305 0.0 6 301 4 185 330 0.0 6 324 6 186 332 0.0 6 331 1 187 281 0.0 6 281 188 258 0.0 6 254 3 0 0 0 1 189 246 0.0 6 244 2 190 293 0.0 6 290 3 191 326 0.0 6 321 5 192 372 0.0 6 370 2 193 416 0.0 6 412 4 194 451 0.0 6 447 3 0 0 0 0 1 195 483 0.0 6 476 6 0 1 196 559 0.0 6 554 4 1 197 604 0.0 6 598 5 1 198 648 0.0 6 641 6 1 199 490 0.0 6 483 7 200 443 0.0 6 441 2 201 452 0.0 6 448 4 202 462 0.0 6 459 3 203 610 0.0 6 604 6 204 460 0.0 6 459 1 205 601 0.0 6 597 4 206 615 0.0 6 604 11 207 557 0.0 6 547 10 208 648 0.0 6 642 6 209 526 0.0 6 515 11 210 459 0.0 6 457 2 211 496 0.0 6 490 4 0 0 0 2 212 535 0.0 6 529 5 0 1 213 592 0.0 6 583 9 214 587 0.0 6 584 3 215 739 0.0 6 734 4 0 0 0 1 216 568 0.0 6 563 5 217 627 0.0 6 624 3 218 614 0.0 6 608 6 219 540 0.0 6 533 7 220 438 0.0 6 432 6 221 527 0.0 6 525 1 0 0 1 222 603 0.0 6 598 5 223 483 0.0 6 479 3 0 0 1 224 471 0.0 6 463 7 1 225 620 0.0 6 611 9 226 926 0.0 6 913 13 227 636 0.0 6 626 10 228 574 0.0 6 572 2 229 546 0.0 6 538 7 0 0 0 1 230 596 0.0 6 588 8 231 529 0.0 6 525 4 232 607 0.0 6 600 7 233 595 0.0 6 584 11 234 551 0.0 6 541 9 0 0 1 235 384 0.0 6 379 4 1 236 410 0.0 6 406 4 237 376 0.0 6 372 4 238 453 0.0 6 447 6 239 444 0.0 6 439 5 240 512 0.0 6 502 9 0 0 0 1 241 650 0.0 6 639 10 1 242 587 0.0 6 576 11 243 560 0.0 6 547 12 1 244 446 0.0 6 439 7 245 622 0.0 6 617 5 246 320 0.0 6 313 7 247 344 0.0 6 338 6 248 278 0.0 6 271 7 249 548 0.0 6 542 5 0 0 1 250 403 0.0 6 396 7 251 477 0.0 6 466 11 252 349 0.0 6 343 6 253 383 0.0 6 372 11 254 730 0.0 6 722 7 1 255 439 0.0 6 431 8 256 395 0.0 6 390 5 257 504 0.0 6 495 9 258 554 0.0 6 541 13 259 802 0.0 6 787 15 260 852 0.0 6 841 11 261 376 0.0 6 372 4 262 346 0.0 6 335 11 263 796 0.0 6 786 10 264 299 0.0 6 294 5 265 512 0.0 6 501 10 0 0 0 0 1 266 330 0.0 6 326 4 267 424 0.0 6 418 6 268 404 0.0 6 398 6 269 526 0.0 6 516 9 0 0 0 0 1 270 257 0.0 6 251 5 1 271 383 0.0 6 376 6 1 272 738 0.0 6 728 10 273 557 0.0 6 546 9 1 1 274 727 0.0 6 716 11 275 766 0.0 6 749 17 276 1080 0.0 6 1059 21 277 1509 0.0 6 1472 37 278 2049 0.0 6 2022 26 0 1 279 4311 0.0 6 4164 138 8 0 1 280 70 0.0 6 63 6 1 281 16 0.0 6 14 1 1 282 1 0.0 6 1 283 2 0.0 6 2 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 896 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 3 0.0 6 3 63 59 0.0 6 57 2 65 1 0.0 6 1 69 1 0.0 6 1 71 2 0.0 6 2 72 9 0.0 6 9 73 13 0.0 6 13 74 16 0.0 6 15 1 75 9 0.0 6 9 76 9 0.0 6 9 77 17 0.0 6 17 78 8 0.0 6 8 79 12 0.0 6 12 80 15 0.0 6 15 81 5 0.0 6 5 82 9 0.0 6 9 83 9 0.0 6 9 84 7 0.0 6 7 85 8 0.0 6 8 86 2 0.0 6 2 87 5 0.0 6 5 88 6 0.0 6 6 89 2 0.0 6 2 90 12 0.0 6 12 91 14 0.0 6 14 92 4 0.0 6 4 93 3 0.0 6 3 94 2 0.0 6 2 95 4 0.0 6 4 96 2 0.0 6 2 97 4 0.0 6 4 98 4 0.0 6 4 99 2 0.0 6 2 100 8 0.0 6 8 101 6 0.0 6 6 102 5 0.0 6 5 103 6 0.0 6 6 104 3 0.0 6 3 105 3 0.0 6 3 107 6 0.0 6 6 108 3 0.0 6 3 109 5 0.0 6 5 110 11 0.0 6 11 111 2 0.0 6 2 112 5 0.0 6 5 113 3 0.0 6 3 115 7 0.0 6 7 116 1 0.0 6 1 117 5 0.0 6 5 118 6 0.0 6 6 119 4 0.0 6 4 120 4 0.0 6 4 121 1 0.0 6 1 122 4 0.0 6 4 123 4 0.0 6 4 124 6 0.0 6 6 125 7 0.0 6 7 126 4 0.0 6 4 127 4 0.0 6 4 128 3 0.0 6 3 129 1 0.0 6 1 131 2 0.0 6 2 132 4 0.0 6 4 133 6 0.0 6 6 134 9 0.0 6 9 135 3 0.0 6 3 136 6 0.0 6 6 137 6 0.0 6 6 138 3 0.0 6 3 139 6 0.0 6 6 140 4 0.0 6 4 141 5 0.0 6 5 142 1 0.0 6 1 143 3 0.0 6 3 144 3 0.0 6 3 145 6 0.0 6 6 146 2 0.0 6 2 147 6 0.0 6 6 148 4 0.0 6 4 149 6 0.0 6 6 150 2 0.0 6 2 151 5 0.0 6 5 152 7 0.0 6 7 153 6 0.0 6 6 154 2 0.0 6 2 155 2 0.0 6 2 156 3 0.0 6 3 157 3 0.0 6 3 158 9 0.0 6 9 159 4 0.0 6 4 160 1 0.0 6 1 161 4 0.0 6 4 162 8 0.0 6 8 163 5 0.0 6 5 164 5 0.0 6 5 165 5 0.0 6 5 166 6 0.0 6 6 167 7 0.0 6 7 168 5 0.0 6 5 169 6 0.0 6 6 170 3 0.0 6 3 171 1 0.0 6 1 172 3 0.0 6 3 173 5 0.0 6 5 174 1 0.0 6 1 175 4 0.0 6 4 176 3 0.0 6 3 177 4 0.0 6 4 178 5 0.0 6 5 179 5 0.0 6 5 180 2 0.0 6 2 181 4 0.0 6 4 182 7 0.0 6 7 183 2 0.0 6 2 184 6 0.0 6 6 185 6 0.0 6 6 186 5 0.0 6 5 187 9 0.0 6 9 188 6 0.0 6 6 189 3 0.0 6 3 190 8 0.0 6 7 1 191 2 0.0 6 2 192 5 0.0 6 5 193 5 0.0 6 5 194 5 0.0 6 5 195 2 0.0 6 2 196 6 0.0 6 6 197 3 0.0 6 3 198 8 0.0 6 8 199 5 0.0 6 5 200 7 0.0 6 7 201 3 0.0 6 3 202 3 0.0 6 3 203 8 0.0 6 8 204 3 0.0 6 3 205 3 0.0 6 3 206 4 0.0 6 4 207 3 0.0 6 3 208 6 0.0 6 6 209 2 0.0 6 2 210 2 0.0 6 2 211 1 0.0 6 1 212 9 0.0 6 9 213 3 0.0 6 3 214 7 0.0 6 7 215 1 0.0 6 1 216 2 0.0 6 2 217 6 0.0 6 6 218 6 0.0 6 6 219 3 0.0 6 3 220 1 0.0 6 1 221 4 0.0 6 4 222 4 0.0 6 4 223 4 0.0 6 4 224 4 0.0 6 4 225 4 0.0 6 4 227 3 0.0 6 3 228 2 0.0 6 2 229 6 0.0 6 6 230 5 0.0 6 5 231 2 0.0 6 2 232 3 0.0 6 3 233 2 0.0 6 2 234 1 0.0 6 1 235 1 0.0 6 1 236 1 0.0 6 1 237 1 0.0 6 1 238 2 0.0 6 2 239 7 0.0 6 7 242 2 0.0 6 2 243 1 0.0 6 1 244 2 0.0 6 2 245 3 0.0 6 3 246 3 0.0 6 3 248 2 0.0 6 2 250 2 0.0 6 2 251 1 0.0 6 1 252 1 0.0 6 1 253 2 0.0 6 2 254 1 0.0 6 1 257 1 0.0 6 1 258 2 0.0 6 2 259 1 0.0 6 1 260 2 0.0 6 2 261 2 0.0 6 2 262 2 0.0 6 2 266 1 0.0 6 1 271 1 0.0 6 1 277 1 0.0 6 1 279 1 0.0 6 1 282 1 0.0 6 1 283 1 0.0 6 1 293 1 0.0 6 1 298 1 0.0 6 1 303 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 70490 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1112.6 0 1 8 1 1.1 2 1 12 2 0.0 3 2 13 13 0.0 3 11 2 14 9 0.0 4 8 1 15 1 0.0 4 1 16 14 0.0 4 14 17 12 0.0 5 12 18 8 0.0 5 7 1 19 13 0.0 5 12 0 1 20 72 0.0 6 71 1 21 53 0.0 6 50 2 0 1 22 34 0.0 6 32 2 23 16 0.0 6 16 24 17 0.0 6 16 1 25 21 0.0 6 19 2 26 20 0.0 6 18 2 27 16 0.0 6 15 1 28 10 0.0 6 10 29 9 0.0 6 8 0 1 30 10 0.0 6 9 1 31 13 0.0 6 13 32 16 0.0 6 16 33 6 0.0 6 5 1 34 5 0.0 6 5 35 12 0.0 6 12 36 9 0.0 6 9 37 10 0.0 6 9 1 38 19 0.0 6 15 4 39 7 0.0 6 7 40 6 0.0 6 6 41 12 0.0 6 12 42 10 0.0 6 10 43 12 0.0 6 9 3 44 13 0.0 6 9 4 45 10 0.0 6 8 2 46 17 0.0 6 17 47 15 0.0 6 14 1 48 16 0.0 6 16 49 16 0.0 6 15 1 50 16 0.0 6 12 4 51 11 0.0 6 11 52 21 0.0 6 19 1 1 53 20 0.0 6 19 1 54 20 0.0 6 17 3 55 21 0.0 6 21 56 24 0.0 6 21 3 57 18 0.0 6 17 1 58 18 0.0 6 18 59 15 0.0 6 15 60 21 0.0 6 17 3 1 61 25 0.0 6 23 2 62 21 0.0 6 20 1 63 27 0.0 6 24 2 0 1 64 21 0.0 6 20 1 65 20 0.0 6 20 66 28 0.0 6 23 4 0 1 67 31 0.0 6 29 2 68 41 0.0 6 36 2 2 1 69 132 0.0 6 128 4 70 49 0.0 6 47 2 71 80 0.0 6 79 1 72 42 0.0 6 39 2 0 0 1 73 56 0.0 6 55 1 74 24 0.0 6 23 1 75 30 0.0 6 28 2 76 35 0.0 6 31 4 77 32 0.0 6 28 3 0 0 1 78 41 0.0 6 39 1 1 79 33 0.0 6 33 80 44 0.0 6 43 1 81 39 0.0 6 39 82 53 0.0 6 47 5 0 0 1 83 50 0.0 6 50 84 66 0.0 6 61 4 1 85 58 0.0 6 55 3 86 43 0.0 6 39 4 87 41 0.0 6 36 5 88 33 0.0 6 30 2 1 89 57 0.0 6 55 2 90 48 0.0 6 46 2 91 49 0.0 6 45 3 0 0 1 92 37 0.0 6 37 93 68 0.0 6 66 2 94 80 0.0 6 76 4 95 61 0.0 6 61 96 65 0.0 6 62 2 0 0 0 0 1 97 68 0.0 6 64 3 1 98 81 0.0 6 78 2 1 99 66 0.0 6 63 3 100 70 0.0 6 68 2 101 69 0.0 6 68 1 102 84 0.0 6 82 1 0 1 103 80 0.0 6 79 1 104 97 0.0 6 93 3 0 0 1 105 107 0.0 6 104 3 106 145 0.0 6 136 4 2 1 2 107 99 0.0 6 97 1 1 108 75 0.0 6 74 1 109 94 0.0 6 90 3 0 0 0 0 1 110 90 0.0 6 86 4 111 95 0.0 6 93 1 1 112 100 0.0 6 97 3 113 95 0.0 6 92 3 114 133 0.0 6 130 2 0 0 1 115 104 0.0 6 100 3 0 0 0 1 116 104 0.0 6 102 2 117 116 0.0 6 110 5 1 118 136 0.0 6 134 2 119 168 0.0 6 165 3 120 192 0.0 6 187 5 121 149 0.0 6 140 6 0 0 1 0 2 122 170 0.0 6 164 5 0 0 0 1 123 187 0.0 6 183 4 124 208 0.0 6 202 4 0 0 0 1 1 125 166 0.0 6 162 4 126 179 0.0 6 175 4 127 229 0.0 6 224 5 128 192 0.0 6 190 2 129 207 0.0 6 198 8 0 1 130 256 0.0 6 251 5 131 334 0.0 6 324 8 1 0 0 0 1 132 343 0.0 6 334 8 1 133 351 0.0 6 345 5 0 1 134 340 0.0 6 330 7 1 0 1 1 135 443 0.0 6 431 10 2 136 402 0.0 6 395 6 0 0 1 137 435 0.0 6 424 9 0 1 1 138 372 0.0 6 367 3 0 0 0 0 2 139 405 0.0 6 399 4 0 0 0 2 140 556 0.0 6 553 2 1 141 668 0.0 6 654 10 1 0 2 1 142 810 0.0 6 797 10 0 0 0 1 2 143 741 0.0 6 720 17 2 0 0 0 2 144 833 0.0 6 824 7 0 0 0 1 1 145 875 0.0 6 866 8 0 0 1 146 857 0.0 6 844 13 147 778 0.0 6 769 9 148 853 0.0 6 839 10 1 0 2 0 1 149 983 0.0 6 974 8 0 0 0 0 1 150 1163 0.0 6 1145 15 0 3 151 968 0.0 6 957 9 0 0 0 1 1 152 1344 0.0 6 1324 19 0 0 0 0 1 153 952 0.0 6 935 15 1 1 154 1073 0.0 6 1058 12 0 0 1 1 1 155 1103 0.0 6 1092 10 0 0 0 0 1 156 904 0.0 6 889 14 0 0 0 0 1 157 1029 0.0 6 1012 16 1 158 943 0.0 6 931 10 1 0 0 1 159 805 0.0 6 790 14 0 0 0 0 1 160 989 0.0 6 975 13 0 0 1 161 824 0.0 6 811 12 0 1 162 803 0.0 6 791 9 0 0 2 1 163 847 0.0 6 831 15 1 164 676 0.0 6 665 9 1 0 1 165 757 0.0 6 750 4 1 1 0 1 166 822 0.0 6 811 7 1 1 2 167 603 0.0 6 597 5 0 0 1 168 952 0.0 6 939 11 0 1 0 1 169 582 0.0 6 575 7 170 816 0.0 6 805 10 1 171 840 0.0 6 829 10 0 1 172 570 0.0 6 556 11 0 1 0 1 1 173 719 0.0 6 702 12 2 2 0 0 1 174 835 0.0 6 816 18 1 175 1107 0.0 6 1088 17 2 176 714 0.0 6 709 5 177 836 0.0 6 827 8 1 178 826 0.0 6 819 6 0 0 0 0 1 179 695 0.0 6 684 8 1 2 180 708 0.0 6 695 12 1 181 778 0.0 6 762 14 0 1 1 182 378 0.0 6 370 7 0 0 1 183 627 0.0 6 619 8 184 416 0.0 6 406 9 1 185 622 0.0 6 614 8 186 1288 0.0 6 1257 30 1 187 1056 0.0 6 1031 19 2 0 4 188 988 0.0 6 976 12 189 826 0.0 6 803 19 2 0 2 190 1130 0.0 6 1108 22 191 939 0.0 6 915 23 0 0 1 192 723 0.0 6 711 12 193 636 0.0 6 619 15 2 194 739 0.0 6 720 17 1 0 1 195 951 0.0 6 929 20 1 0 1 196 1550 0.0 6 1500 48 1 0 1 197 353 0.0 6 341 11 1 198 1256 0.0 6 1228 27 0 0 1 199 1778 0.0 6 1745 32 0 0 1 200 495 0.0 6 477 16 0 0 2 201 624 0.0 6 605 16 1 1 0 1 202 398 0.0 6 391 5 1 0 0 1 203 413 0.0 6 403 10 204 423 0.0 6 413 10 205 182 0.0 6 176 5 0 0 0 0 1 206 186 0.0 6 183 3 207 151 0.0 6 145 6 208 249 0.0 6 244 5 209 183 0.0 6 183 210 180 0.0 6 174 6 211 227 0.0 6 222 5 212 216 0.0 6 214 2 213 74 0.0 6 72 1 0 1 214 139 0.0 6 135 4 215 142 0.0 6 137 5 216 444 0.0 6 436 8 217 273 0.0 6 270 3 218 198 0.0 6 192 6 219 150 0.0 6 147 3 220 158 0.0 6 152 6 221 180 0.0 6 174 5 1 222 283 0.0 6 276 7 223 593 0.0 6 582 10 0 0 1 224 109 0.0 6 107 2 225 202 0.0 6 197 5 226 120 0.0 6 117 3 227 129 0.0 6 127 1 0 1 228 49 0.0 6 47 2 229 90 0.0 6 90 230 64 0.0 6 63 1 231 72 0.0 6 71 1 232 90 0.0 6 88 2 233 185 0.0 6 177 8 234 69 0.0 6 69 235 31 0.0 6 31 236 22 0.0 6 22 237 12 0.0 6 12 238 7 0.0 6 7 239 8 0.0 6 8 240 58 0.0 6 57 1 241 36 0.0 6 36 242 77 0.0 6 73 4 243 89 0.0 6 86 3 244 36 0.0 6 36 245 9 0.0 6 9 246 15 0.0 6 15 247 6 0.0 6 6 248 16 0.0 6 16 249 12 0.0 6 11 1 250 17 0.0 6 16 1 251 11 0.0 6 11 252 2 0.0 6 1 1 253 13 0.0 6 13 254 4 0.0 6 4 256 1 0.0 6 1 276 1 0.0 6 1 278 1 0.0 6 1 279 8 0.0 6 8 280 26 0.0 6 26 281 161 0.0 6 154 7 283 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 687 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 51 1 0.0 6 1 53 1 0.0 6 1 54 1 0.0 6 1 55 1 0.0 6 1 63 1 0.0 6 1 65 3 0.0 6 3 66 1 0.0 6 1 67 5 0.0 6 5 68 1 0.0 6 1 69 1 0.0 6 1 70 1 0.0 6 1 71 8 0.0 6 8 72 18 0.0 6 18 73 9 0.0 6 9 74 12 0.0 6 12 75 14 0.0 6 14 76 8 0.0 6 8 77 6 0.0 6 6 78 12 0.0 6 12 79 18 0.0 6 18 80 10 0.0 6 10 81 11 0.0 6 11 82 12 0.0 6 12 83 4 0.0 6 4 84 2 0.0 6 2 85 4 0.0 6 4 86 6 0.0 6 6 87 5 0.0 6 5 88 7 0.0 6 7 89 5 0.0 6 5 90 9 0.0 6 9 91 5 0.0 6 5 92 3 0.0 6 3 93 8 0.0 6 8 94 5 0.0 6 5 95 7 0.0 6 7 96 2 0.0 6 2 97 4 0.0 6 4 98 5 0.0 6 5 99 6 0.0 6 6 100 5 0.0 6 4 0 0 1 101 1 0.0 6 1 102 6 0.0 6 5 1 103 3 0.0 6 3 104 4 0.0 6 4 105 3 0.0 6 3 106 3 0.0 6 3 107 6 0.0 6 6 108 11 0.0 6 11 109 4 0.0 6 4 110 5 0.0 6 5 111 6 0.0 6 6 112 6 0.0 6 6 113 4 0.0 6 4 114 3 0.0 6 3 115 2 0.0 6 2 116 2 0.0 6 2 117 4 0.0 6 4 118 4 0.0 6 4 120 5 0.0 6 5 121 5 0.0 6 5 122 5 0.0 6 5 123 5 0.0 6 5 124 9 0.0 6 9 125 5 0.0 6 5 126 7 0.0 6 7 127 4 0.0 6 4 128 4 0.0 6 4 129 1 0.0 6 1 130 3 0.0 6 3 131 3 0.0 6 3 132 4 0.0 6 4 133 4 0.0 6 4 134 5 0.0 6 5 135 2 0.0 6 2 136 5 0.0 6 5 137 4 0.0 6 3 1 138 2 0.0 6 2 139 10 0.0 6 10 140 7 0.0 6 7 141 1 0.0 6 1 142 10 0.0 6 10 143 8 0.0 6 8 144 8 0.0 6 8 145 4 0.0 6 4 146 4 0.0 6 4 147 9 0.0 6 9 148 4 0.0 6 4 149 10 0.0 6 10 150 14 0.0 6 14 152 5 0.0 6 5 153 5 0.0 6 5 154 4 0.0 6 4 155 3 0.0 6 3 156 5 0.0 6 5 157 4 0.0 6 4 158 3 0.0 6 3 159 6 0.0 6 6 160 9 0.0 6 9 161 3 0.0 6 3 162 12 0.0 6 12 163 1 0.0 6 1 164 2 0.0 6 2 165 3 0.0 6 3 166 6 0.0 6 6 167 2 0.0 6 2 168 7 0.0 6 7 169 3 0.0 6 3 170 1 0.0 6 1 171 2 0.0 6 2 172 6 0.0 6 6 173 4 0.0 6 4 174 6 0.0 6 6 176 5 0.0 6 5 177 5 0.0 6 5 178 3 0.0 6 3 179 4 0.0 6 4 180 2 0.0 6 2 181 4 0.0 6 4 182 5 0.0 6 5 183 6 0.0 6 6 184 1 0.0 6 1 185 5 0.0 6 5 186 5 0.0 6 5 187 3 0.0 6 3 188 2 0.0 6 2 189 2 0.0 6 2 190 4 0.0 6 4 191 2 0.0 6 2 193 1 0.0 6 1 194 1 0.0 6 1 196 2 0.0 6 2 197 1 0.0 6 1 198 1 0.0 6 1 200 1 0.0 6 1 201 2 0.0 6 2 202 1 0.0 6 1 203 1 0.0 6 1 205 4 0.0 6 4 206 1 0.0 6 1 208 1 0.0 6 1 210 2 0.0 6 2 211 1 0.0 6 1 212 1 0.0 6 1 214 1 0.0 6 1 217 1 0.0 6 1 221 1 0.0 6 1 225 1 0.0 6 1 226 1 0.0 6 1 229 1 0.0 6 1 232 2 0.0 6 2 242 1 0.0 6 1 243 1 0.0 6 1 246 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_06_113_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_06_340_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_06_113_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_06_340_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.11 s (38 µs/read; 1.58 M reads/minute). === Summary === Total read pairs processed: 55,728 Read 1 with adapter: 55,720 (100.0%) Read 2 with adapter: 55,710 (100.0%) == Read fate breakdown == Pairs that were too short: 39 (0.1%) Pairs discarded as untrimmed: 26 (0.0%) Pairs written (passing filters): 55,663 (99.9%) Total basepairs processed: 23,903,346 bp Read 1: 13,453,058 bp Read 2: 10,450,288 bp Total written (filtered): 3,487,605 bp (14.6%) Read 1: 1,842,177 bp Read 2: 1,645,428 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 54312 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 870.8 0 0 3 4 4 217.7 1 1 3 6 1 13.6 1 0 0 1 7 5 3.4 2 1 0 4 8 1 0.9 2 1 9 1 0.2 2 1 15 1 0.0 4 1 17 6 0.0 5 5 1 19 1 0.0 5 1 20 60 0.0 6 60 21 36 0.0 6 35 1 22 38 0.0 6 38 23 26 0.0 6 25 1 24 15 0.0 6 13 2 25 21 0.0 6 20 0 1 26 13 0.0 6 13 27 7 0.0 6 7 28 7 0.0 6 5 2 29 18 0.0 6 17 1 30 7 0.0 6 7 31 9 0.0 6 8 0 0 1 32 3 0.0 6 3 33 5 0.0 6 3 1 1 34 4 0.0 6 3 1 35 1 0.0 6 1 36 3 0.0 6 2 1 37 4 0.0 6 3 1 38 3 0.0 6 2 0 0 1 39 11 0.0 6 9 2 40 3 0.0 6 3 41 5 0.0 6 5 42 7 0.0 6 7 43 7 0.0 6 7 44 7 0.0 6 7 45 6 0.0 6 5 1 46 3 0.0 6 3 47 5 0.0 6 5 48 3 0.0 6 3 49 5 0.0 6 5 50 4 0.0 6 4 51 6 0.0 6 6 52 7 0.0 6 7 53 6 0.0 6 6 54 7 0.0 6 5 2 55 2 0.0 6 2 56 5 0.0 6 5 57 4 0.0 6 4 58 3 0.0 6 3 59 6 0.0 6 6 60 8 0.0 6 8 61 5 0.0 6 5 62 4 0.0 6 3 1 63 8 0.0 6 8 64 8 0.0 6 8 65 11 0.0 6 10 1 66 9 0.0 6 9 67 6 0.0 6 6 68 8 0.0 6 7 1 69 5 0.0 6 5 70 13 0.0 6 11 1 0 0 0 0 1 71 5 0.0 6 5 72 3 0.0 6 3 73 6 0.0 6 6 74 7 0.0 6 7 75 5 0.0 6 5 76 5 0.0 6 5 77 7 0.0 6 6 0 1 78 10 0.0 6 9 1 79 7 0.0 6 7 80 8 0.0 6 8 81 9 0.0 6 9 82 7 0.0 6 7 83 5 0.0 6 5 84 6 0.0 6 5 1 85 7 0.0 6 7 86 7 0.0 6 5 2 87 9 0.0 6 8 1 88 13 0.0 6 13 89 7 0.0 6 7 90 9 0.0 6 9 91 6 0.0 6 6 92 5 0.0 6 5 93 10 0.0 6 10 94 5 0.0 6 5 95 11 0.0 6 10 1 96 5 0.0 6 4 1 97 13 0.0 6 13 98 9 0.0 6 9 99 7 0.0 6 7 100 13 0.0 6 13 101 9 0.0 6 9 102 14 0.0 6 14 103 13 0.0 6 13 104 15 0.0 6 15 105 15 0.0 6 14 0 0 0 0 0 1 106 13 0.0 6 13 107 15 0.0 6 15 108 17 0.0 6 16 1 109 11 0.0 6 11 110 18 0.0 6 18 111 19 0.0 6 19 112 17 0.0 6 17 113 17 0.0 6 17 114 13 0.0 6 13 115 15 0.0 6 12 2 1 116 17 0.0 6 17 117 14 0.0 6 12 2 118 24 0.0 6 21 3 119 20 0.0 6 20 120 21 0.0 6 21 121 20 0.0 6 19 1 122 23 0.0 6 23 123 21 0.0 6 19 2 124 17 0.0 6 17 125 24 0.0 6 24 126 27 0.0 6 26 0 0 0 0 1 127 22 0.0 6 22 128 20 0.0 6 20 129 26 0.0 6 26 130 25 0.0 6 24 1 131 30 0.0 6 29 0 0 0 0 0 1 132 26 0.0 6 25 1 133 33 0.0 6 33 134 38 0.0 6 36 2 135 29 0.0 6 29 136 32 0.0 6 31 1 137 35 0.0 6 35 138 43 0.0 6 43 139 62 0.0 6 61 1 140 114 0.0 6 113 1 141 188 0.0 6 187 1 142 336 0.0 6 335 1 143 302 0.0 6 300 2 144 257 0.0 6 257 145 304 0.0 6 304 146 358 0.0 6 356 1 0 1 147 388 0.0 6 388 148 336 0.0 6 335 1 149 276 0.0 6 274 1 0 0 0 1 150 346 0.0 6 345 1 151 308 0.0 6 307 1 152 244 0.0 6 243 1 153 182 0.0 6 182 154 253 0.0 6 252 0 1 155 221 0.0 6 217 4 156 196 0.0 6 194 2 157 262 0.0 6 262 158 303 0.0 6 302 1 159 256 0.0 6 256 160 314 0.0 6 311 2 0 0 1 161 343 0.0 6 341 2 162 295 0.0 6 294 0 1 163 241 0.0 6 239 2 164 186 0.0 6 183 3 165 223 0.0 6 220 2 0 0 1 166 243 0.0 6 241 2 167 239 0.0 6 236 3 168 258 0.0 6 255 1 1 0 0 1 169 255 0.0 6 253 2 170 296 0.0 6 295 1 171 284 0.0 6 284 172 252 0.0 6 249 3 173 309 0.0 6 308 1 174 227 0.0 6 225 1 0 0 1 175 232 0.0 6 229 2 1 176 243 0.0 6 242 1 177 268 0.0 6 266 2 178 426 0.0 6 423 2 0 1 179 268 0.0 6 266 2 180 214 0.0 6 212 2 181 197 0.0 6 193 3 0 0 0 0 1 182 219 0.0 6 216 3 183 326 0.0 6 323 3 184 304 0.0 6 298 6 185 343 0.0 6 339 3 0 1 186 253 0.0 6 249 4 187 296 0.0 6 292 4 188 309 0.0 6 307 1 0 1 189 240 0.0 6 237 3 190 300 0.0 6 299 1 191 270 0.0 6 268 2 192 347 0.0 6 342 4 0 0 0 1 193 369 0.0 6 364 4 0 0 0 0 1 194 381 0.0 6 376 4 1 195 426 0.0 6 423 2 0 0 0 0 1 196 574 0.0 6 570 3 0 1 197 565 0.0 6 557 7 0 0 0 0 1 198 622 0.0 6 614 8 199 439 0.0 6 435 4 200 366 0.0 6 363 2 0 0 1 201 423 0.0 6 418 3 2 202 472 0.0 6 469 1 0 0 1 1 203 405 0.0 6 401 3 1 204 500 0.0 6 493 5 1 0 0 0 1 205 518 0.0 6 510 7 0 0 0 1 206 695 0.0 6 690 5 207 587 0.0 6 584 3 208 574 0.0 6 566 7 1 209 539 0.0 6 538 1 210 575 0.0 6 565 10 211 578 0.0 6 573 3 1 0 0 1 212 668 0.0 6 663 5 213 633 0.0 6 627 6 214 717 0.0 6 710 6 0 0 0 1 215 856 0.0 6 849 6 1 216 624 0.0 6 618 6 217 657 0.0 6 651 6 218 772 0.0 6 762 9 1 219 592 0.0 6 589 3 220 488 0.0 6 485 3 221 521 0.0 6 515 6 222 517 0.0 6 511 6 223 622 0.0 6 616 6 224 594 0.0 6 589 5 225 686 0.0 6 679 7 226 645 0.0 6 640 5 227 502 0.0 6 500 1 0 1 228 563 0.0 6 555 7 0 0 1 229 517 0.0 6 512 5 230 551 0.0 6 547 3 1 231 550 0.0 6 545 4 0 0 0 1 232 524 0.0 6 514 7 1 1 0 0 1 233 501 0.0 6 496 3 1 1 234 487 0.0 6 481 5 0 0 0 1 235 419 0.0 6 412 6 0 0 1 236 527 0.0 6 524 1 0 0 0 0 2 237 481 0.0 6 475 5 0 0 1 238 524 0.0 6 521 3 239 465 0.0 6 465 240 426 0.0 6 423 3 241 389 0.0 6 388 1 242 451 0.0 6 448 3 243 463 0.0 6 457 3 2 0 0 1 244 415 0.0 6 409 4 2 245 319 0.0 6 317 2 246 278 0.0 6 278 247 290 0.0 6 288 2 248 283 0.0 6 282 0 1 249 294 0.0 6 293 1 250 323 0.0 6 319 3 1 251 255 0.0 6 254 0 1 252 240 0.0 6 237 3 253 264 0.0 6 262 2 254 241 0.0 6 238 3 255 236 0.0 6 235 1 256 237 0.0 6 236 1 257 229 0.0 6 229 258 232 0.0 6 231 1 259 271 0.0 6 271 260 188 0.0 6 188 261 180 0.0 6 179 1 262 157 0.0 6 156 1 263 195 0.0 6 194 1 264 194 0.0 6 191 3 265 193 0.0 6 191 2 266 154 0.0 6 153 1 267 176 0.0 6 174 2 268 173 0.0 6 172 0 0 1 269 202 0.0 6 202 270 157 0.0 6 155 2 271 131 0.0 6 131 272 138 0.0 6 138 273 172 0.0 6 172 274 205 0.0 6 203 2 275 213 0.0 6 210 3 276 266 0.0 6 266 277 367 0.0 6 366 1 278 1165 0.0 6 1156 9 279 1679 0.0 6 1661 15 0 1 1 0 1 280 9 0.0 6 8 0 0 1 281 4 0.0 6 4 282 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1408 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 1 0.0 6 0 1 63 59 0.0 6 59 71 10 0.0 6 10 72 5 0.0 6 5 73 4 0.0 6 3 1 74 11 0.0 6 11 75 11 0.0 6 11 76 6 0.0 6 6 77 8 0.0 6 8 78 10 0.0 6 10 79 17 0.0 6 17 80 11 0.0 6 11 81 12 0.0 6 12 82 23 0.0 6 23 83 15 0.0 6 15 84 8 0.0 6 8 85 5 0.0 6 5 86 9 0.0 6 9 87 2 0.0 6 2 88 4 0.0 6 4 89 6 0.0 6 6 90 6 0.0 6 6 91 11 0.0 6 11 92 5 0.0 6 5 93 13 0.0 6 13 94 6 0.0 6 6 95 5 0.0 6 5 96 3 0.0 6 3 97 4 0.0 6 4 98 11 0.0 6 11 99 1 0.0 6 1 100 6 0.0 6 6 101 12 0.0 6 12 102 7 0.0 6 7 103 4 0.0 6 4 104 10 0.0 6 10 105 7 0.0 6 7 106 8 0.0 6 8 107 6 0.0 6 6 108 8 0.0 6 8 109 6 0.0 6 6 110 16 0.0 6 16 111 7 0.0 6 7 112 4 0.0 6 4 113 5 0.0 6 5 114 4 0.0 6 4 115 10 0.0 6 10 116 5 0.0 6 5 117 8 0.0 6 8 118 5 0.0 6 5 119 2 0.0 6 2 120 3 0.0 6 3 121 5 0.0 6 5 122 7 0.0 6 7 123 9 0.0 6 9 124 7 0.0 6 7 125 3 0.0 6 3 126 1 0.0 6 1 127 5 0.0 6 5 128 4 0.0 6 4 129 4 0.0 6 4 130 2 0.0 6 2 131 2 0.0 6 2 132 3 0.0 6 2 1 133 6 0.0 6 6 134 9 0.0 6 9 135 7 0.0 6 7 136 3 0.0 6 3 137 2 0.0 6 2 138 11 0.0 6 11 139 6 0.0 6 6 140 10 0.0 6 10 141 7 0.0 6 7 142 7 0.0 6 7 143 8 0.0 6 8 144 3 0.0 6 3 145 1 0.0 6 1 146 3 0.0 6 3 147 3 0.0 6 3 148 5 0.0 6 5 149 10 0.0 6 10 150 14 0.0 6 14 151 6 0.0 6 6 152 8 0.0 6 8 153 9 0.0 6 9 154 3 0.0 6 3 155 3 0.0 6 3 156 4 0.0 6 4 157 5 0.0 6 5 158 4 0.0 6 4 159 5 0.0 6 5 160 5 0.0 6 5 161 5 0.0 6 5 162 7 0.0 6 7 163 15 0.0 6 15 164 4 0.0 6 4 165 5 0.0 6 5 166 8 0.0 6 8 167 6 0.0 6 6 168 7 0.0 6 7 169 6 0.0 6 6 170 5 0.0 6 5 171 4 0.0 6 4 172 8 0.0 6 8 173 9 0.0 6 9 174 9 0.0 6 9 175 9 0.0 6 9 176 6 0.0 6 6 177 7 0.0 6 7 178 9 0.0 6 9 179 14 0.0 6 14 180 2 0.0 6 2 181 4 0.0 6 4 182 4 0.0 6 4 183 5 0.0 6 5 184 7 0.0 6 7 185 8 0.0 6 7 0 0 0 0 0 1 186 8 0.0 6 8 187 9 0.0 6 9 188 12 0.0 6 12 189 17 0.0 6 17 190 5 0.0 6 5 191 16 0.0 6 16 192 11 0.0 6 11 193 5 0.0 6 5 194 4 0.0 6 4 195 8 0.0 6 8 196 10 0.0 6 10 197 5 0.0 6 5 198 6 0.0 6 6 199 7 0.0 6 7 200 9 0.0 6 9 201 11 0.0 6 11 202 8 0.0 6 8 203 26 0.0 6 26 204 5 0.0 6 5 205 8 0.0 6 8 206 11 0.0 6 11 207 6 0.0 6 6 208 6 0.0 6 6 209 10 0.0 6 10 210 9 0.0 6 9 211 11 0.0 6 11 212 14 0.0 6 14 213 11 0.0 6 11 214 14 0.0 6 14 215 11 0.0 6 11 216 3 0.0 6 3 217 8 0.0 6 8 218 10 0.0 6 10 219 11 0.0 6 11 220 17 0.0 6 16 1 221 8 0.0 6 7 1 222 9 0.0 6 9 223 3 0.0 6 3 224 6 0.0 6 6 225 3 0.0 6 3 226 5 0.0 6 5 227 8 0.0 6 8 228 5 0.0 6 5 229 9 0.0 6 9 230 2 0.0 6 2 231 3 0.0 6 3 232 5 0.0 6 5 233 3 0.0 6 3 234 3 0.0 6 3 235 4 0.0 6 4 236 2 0.0 6 2 237 2 0.0 6 2 238 4 0.0 6 4 239 3 0.0 6 3 240 4 0.0 6 4 241 2 0.0 6 2 242 8 0.0 6 8 243 10 0.0 6 10 244 8 0.0 6 8 245 4 0.0 6 4 246 2 0.0 6 2 247 6 0.0 6 6 248 5 0.0 6 5 249 6 0.0 6 6 251 3 0.0 6 3 252 2 0.0 6 2 253 2 0.0 6 2 254 3 0.0 6 3 255 7 0.0 6 6 1 256 4 0.0 6 4 257 2 0.0 6 2 258 4 0.0 6 4 259 3 0.0 6 3 260 9 0.0 6 9 261 3 0.0 6 3 262 2 0.0 6 2 266 2 0.0 6 2 267 5 0.0 6 4 1 268 1 0.0 6 1 269 5 0.0 6 5 270 1 0.0 6 1 272 4 0.0 6 4 273 1 0.0 6 1 274 2 0.0 6 2 275 2 0.0 6 2 278 1 0.0 6 1 279 1 0.0 6 1 280 1 0.0 6 1 281 3 0.0 6 3 282 2 0.0 6 2 284 2 0.0 6 2 285 1 0.0 6 1 290 1 0.0 6 1 293 1 0.0 6 1 294 2 0.0 6 2 295 1 0.0 6 1 298 1 0.0 6 1 300 1 0.0 6 1 303 1 0.0 6 1 305 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 54734 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 3 3.4 2 3 8 1 0.9 2 0 1 9 1 0.2 2 1 11 1 0.0 3 1 12 6 0.0 3 6 13 6 0.0 3 3 1 0 2 14 8 0.0 4 7 1 16 2 0.0 4 2 17 15 0.0 5 14 1 18 3 0.0 5 2 1 19 37 0.0 5 33 3 1 20 89 0.0 6 85 3 1 21 38 0.0 6 38 22 30 0.0 6 28 2 23 31 0.0 6 29 0 2 24 18 0.0 6 18 25 7 0.0 6 6 1 26 24 0.0 6 23 1 27 6 0.0 6 6 28 11 0.0 6 10 1 29 11 0.0 6 11 30 17 0.0 6 17 31 18 0.0 6 16 2 32 9 0.0 6 9 33 13 0.0 6 12 0 0 0 0 0 1 34 11 0.0 6 9 1 0 1 35 9 0.0 6 9 36 7 0.0 6 5 1 0 0 0 0 1 37 9 0.0 6 9 38 16 0.0 6 14 1 0 0 0 0 1 39 14 0.0 6 14 40 6 0.0 6 6 41 14 0.0 6 13 1 42 21 0.0 6 20 1 43 12 0.0 6 11 0 1 44 13 0.0 6 12 0 0 0 0 0 1 45 10 0.0 6 10 46 11 0.0 6 11 47 9 0.0 6 8 1 48 19 0.0 6 18 1 49 16 0.0 6 13 2 1 50 26 0.0 6 24 2 51 22 0.0 6 19 3 52 24 0.0 6 21 2 0 0 0 0 1 53 15 0.0 6 15 54 15 0.0 6 14 1 55 22 0.0 6 20 1 0 0 0 1 56 23 0.0 6 21 2 57 10 0.0 6 10 58 15 0.0 6 13 1 1 59 16 0.0 6 14 2 60 14 0.0 6 14 61 26 0.0 6 23 2 0 0 0 0 1 62 18 0.0 6 17 0 1 63 16 0.0 6 15 1 64 16 0.0 6 14 2 65 17 0.0 6 15 1 0 0 0 1 66 33 0.0 6 32 1 67 27 0.0 6 25 0 0 0 0 0 2 68 37 0.0 6 33 4 69 44 0.0 6 42 2 70 114 0.0 6 108 4 0 1 0 0 1 71 57 0.0 6 55 2 72 55 0.0 6 50 4 1 73 31 0.0 6 31 74 33 0.0 6 29 3 0 0 1 75 25 0.0 6 22 2 0 0 1 76 30 0.0 6 29 1 77 37 0.0 6 34 3 78 34 0.0 6 32 0 1 0 0 0 1 79 26 0.0 6 22 4 80 29 0.0 6 27 1 0 0 1 81 48 0.0 6 45 3 82 34 0.0 6 33 1 83 49 0.0 6 45 2 2 84 46 0.0 6 43 3 85 52 0.0 6 50 2 86 48 0.0 6 47 0 1 87 44 0.0 6 41 3 88 34 0.0 6 32 1 1 89 34 0.0 6 33 0 1 90 43 0.0 6 40 3 91 48 0.0 6 41 4 1 1 0 1 92 49 0.0 6 46 1 0 0 1 0 1 93 57 0.0 6 53 3 0 1 94 50 0.0 6 48 2 95 50 0.0 6 47 2 0 0 0 0 1 96 71 0.0 6 68 3 97 58 0.0 6 57 0 0 1 98 75 0.0 6 71 3 0 0 1 99 74 0.0 6 66 6 1 0 0 1 100 83 0.0 6 81 1 0 0 0 0 1 101 72 0.0 6 66 6 102 64 0.0 6 62 1 1 103 63 0.0 6 60 3 104 77 0.0 6 73 3 0 0 1 105 83 0.0 6 79 3 1 106 87 0.0 6 82 4 0 0 0 0 1 107 155 0.0 6 151 3 0 0 0 1 108 98 0.0 6 94 3 0 0 1 109 83 0.0 6 80 3 110 82 0.0 6 80 2 111 102 0.0 6 101 1 112 111 0.0 6 108 3 113 104 0.0 6 100 2 1 0 0 1 114 127 0.0 6 122 2 0 0 0 1 2 115 129 0.0 6 118 7 2 0 0 0 2 116 136 0.0 6 134 2 117 152 0.0 6 148 3 0 0 0 1 118 157 0.0 6 152 3 1 0 0 1 119 149 0.0 6 147 2 120 167 0.0 6 160 5 0 0 0 2 121 161 0.0 6 158 2 0 0 1 122 147 0.0 6 145 1 0 0 0 0 1 123 141 0.0 6 139 2 124 156 0.0 6 154 2 125 166 0.0 6 164 2 126 185 0.0 6 179 4 1 0 1 127 216 0.0 6 210 3 1 0 1 1 128 251 0.0 6 243 8 129 247 0.0 6 244 3 130 287 0.0 6 269 14 3 0 0 1 131 306 0.0 6 297 6 0 0 3 132 270 0.0 6 264 5 0 0 0 1 133 309 0.0 6 304 5 134 352 0.0 6 342 8 0 0 0 2 135 323 0.0 6 311 10 0 0 1 0 1 136 401 0.0 6 391 8 0 1 0 1 137 447 0.0 6 435 11 1 138 386 0.0 6 375 9 2 139 403 0.0 6 396 5 0 1 0 0 1 140 488 0.0 6 482 4 2 141 498 0.0 6 484 11 1 0 0 1 1 142 669 0.0 6 652 15 0 0 1 0 1 143 712 0.0 6 698 8 2 0 3 0 1 144 697 0.0 6 679 16 0 1 0 0 1 145 754 0.0 6 740 12 1 0 1 146 762 0.0 6 744 14 0 0 1 2 1 147 811 0.0 6 793 13 3 1 0 0 1 148 814 0.0 6 804 9 1 149 792 0.0 6 780 7 2 0 2 0 1 150 856 0.0 6 839 13 1 1 1 1 151 1041 0.0 6 1024 14 1 0 1 0 1 152 1020 0.0 6 988 27 1 0 2 1 1 153 1235 0.0 6 1198 30 3 2 0 1 1 154 997 0.0 6 976 17 2 0 1 0 1 155 1217 0.0 6 1191 23 1 1 1 156 1108 0.0 6 1077 26 1 0 3 0 1 157 1006 0.0 6 989 13 2 1 0 1 158 1009 0.0 6 986 17 0 3 1 1 1 159 920 0.0 6 904 14 0 0 1 0 1 160 1022 0.0 6 1002 17 1 1 1 161 1035 0.0 6 1011 20 0 1 0 1 2 162 823 0.0 6 805 14 2 0 0 1 1 163 793 0.0 6 776 12 2 1 0 1 1 164 759 0.0 6 748 8 1 0 2 165 702 0.0 6 689 10 0 0 3 166 735 0.0 6 718 12 1 1 3 167 735 0.0 6 718 16 0 1 168 690 0.0 6 677 10 0 2 1 169 858 0.0 6 844 13 0 1 170 666 0.0 6 655 8 1 1 0 1 171 803 0.0 6 789 12 0 0 0 0 2 172 698 0.0 6 690 6 0 1 0 0 1 173 722 0.0 6 708 11 1 1 0 1 174 744 0.0 6 734 9 0 1 175 752 0.0 6 738 10 0 2 0 1 1 176 793 0.0 6 778 12 1 0 1 0 1 177 748 0.0 6 737 11 178 668 0.0 6 655 10 1 1 0 1 179 663 0.0 6 649 11 2 0 1 180 541 0.0 6 535 5 0 0 0 0 1 181 536 0.0 6 533 2 0 0 1 182 424 0.0 6 421 2 0 0 1 183 420 0.0 6 417 1 0 1 1 184 392 0.0 6 390 2 185 407 0.0 6 400 5 0 0 0 1 1 186 679 0.0 6 666 11 0 0 0 2 187 767 0.0 6 763 4 188 653 0.0 6 645 8 189 479 0.0 6 475 4 190 461 0.0 6 452 9 191 418 0.0 6 413 5 192 401 0.0 6 398 2 1 193 331 0.0 6 328 3 194 341 0.0 6 332 9 195 293 0.0 6 290 2 1 196 321 0.0 6 315 5 0 0 0 1 197 280 0.0 6 275 4 0 0 0 1 198 210 0.0 6 207 3 199 326 0.0 6 323 3 200 249 0.0 6 245 4 201 246 0.0 6 243 3 202 230 0.0 6 230 203 181 0.0 6 177 3 0 0 1 204 177 0.0 6 176 1 205 159 0.0 6 159 206 147 0.0 6 145 2 207 129 0.0 6 129 208 148 0.0 6 147 1 209 131 0.0 6 129 0 0 0 1 0 1 210 109 0.0 6 107 2 211 114 0.0 6 114 212 102 0.0 6 101 1 213 76 0.0 6 76 214 76 0.0 6 75 1 215 88 0.0 6 88 216 79 0.0 6 79 217 118 0.0 6 117 1 218 69 0.0 6 67 1 1 219 55 0.0 6 54 1 220 64 0.0 6 64 221 56 0.0 6 56 222 38 0.0 6 37 1 223 34 0.0 6 34 224 36 0.0 6 36 225 43 0.0 6 43 226 22 0.0 6 22 227 29 0.0 6 29 228 25 0.0 6 25 229 24 0.0 6 23 1 230 13 0.0 6 13 231 22 0.0 6 21 1 232 14 0.0 6 14 233 19 0.0 6 19 234 13 0.0 6 13 235 7 0.0 6 7 236 4 0.0 6 4 237 1 0.0 6 1 238 4 0.0 6 4 239 2 0.0 6 2 240 2 0.0 6 2 241 13 0.0 6 13 242 4 0.0 6 4 243 5 0.0 6 4 0 0 0 0 0 1 244 11 0.0 6 11 245 1 0.0 6 1 246 2 0.0 6 2 248 3 0.0 6 3 249 2 0.0 6 2 250 2 0.0 6 2 251 3 0.0 6 3 254 6 0.0 6 6 257 1 0.0 6 1 262 1 0.0 6 1 268 1 0.0 6 1 280 1 0.0 6 1 281 13 0.0 6 13 282 105 0.0 6 103 2 283 1 0.0 6 1 286 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 976 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 57 1 0.0 6 1 66 1 0.0 6 1 68 1 0.0 6 1 69 2 0.0 6 2 70 7 0.0 6 7 71 6 0.0 6 6 72 10 0.0 6 10 73 4 0.0 6 4 74 10 0.0 6 10 75 19 0.0 6 19 76 23 0.0 6 23 77 9 0.0 6 9 78 8 0.0 6 8 79 13 0.0 6 13 80 16 0.0 6 16 81 13 0.0 6 13 82 6 0.0 6 6 83 9 0.0 6 9 84 7 0.0 6 7 85 5 0.0 6 5 86 13 0.0 6 13 87 5 0.0 6 5 88 8 0.0 6 8 89 9 0.0 6 9 90 8 0.0 6 8 91 10 0.0 6 10 92 8 0.0 6 8 93 4 0.0 6 4 94 8 0.0 6 8 95 8 0.0 6 8 96 5 0.0 6 5 97 11 0.0 6 11 98 8 0.0 6 8 99 8 0.0 6 8 100 7 0.0 6 7 101 9 0.0 6 9 102 9 0.0 6 9 103 3 0.0 6 3 104 3 0.0 6 3 105 4 0.0 6 4 106 11 0.0 6 11 107 10 0.0 6 10 108 5 0.0 6 5 109 5 0.0 6 5 110 5 0.0 6 5 111 5 0.0 6 5 112 10 0.0 6 10 113 5 0.0 6 5 114 10 0.0 6 10 115 3 0.0 6 3 116 6 0.0 6 5 1 117 10 0.0 6 10 118 7 0.0 6 7 119 5 0.0 6 5 120 9 0.0 6 9 121 7 0.0 6 7 122 5 0.0 6 5 123 4 0.0 6 4 124 4 0.0 6 3 0 0 0 1 125 7 0.0 6 7 126 3 0.0 6 3 127 5 0.0 6 5 128 7 0.0 6 7 129 2 0.0 6 2 130 6 0.0 6 6 131 14 0.0 6 14 132 2 0.0 6 2 133 3 0.0 6 3 134 3 0.0 6 3 135 5 0.0 6 5 136 1 0.0 6 1 137 6 0.0 6 6 138 9 0.0 6 9 139 14 0.0 6 14 140 4 0.0 6 4 141 8 0.0 6 8 142 3 0.0 6 3 143 5 0.0 6 5 144 2 0.0 6 2 145 6 0.0 6 6 146 9 0.0 6 9 147 4 0.0 6 4 148 4 0.0 6 4 149 5 0.0 6 5 150 8 0.0 6 8 151 3 0.0 6 3 152 8 0.0 6 8 153 3 0.0 6 3 154 6 0.0 6 6 155 8 0.0 6 8 156 10 0.0 6 10 157 6 0.0 6 6 158 3 0.0 6 3 159 11 0.0 6 10 0 0 1 160 9 0.0 6 9 161 9 0.0 6 9 162 2 0.0 6 2 163 3 0.0 6 3 164 9 0.0 6 9 165 7 0.0 6 7 166 6 0.0 6 6 167 14 0.0 6 13 0 1 168 2 0.0 6 2 169 5 0.0 6 5 170 7 0.0 6 7 171 11 0.0 6 11 172 12 0.0 6 12 173 5 0.0 6 5 174 9 0.0 6 9 175 5 0.0 6 5 176 6 0.0 6 6 177 1 0.0 6 1 178 6 0.0 6 6 179 4 0.0 6 4 180 13 0.0 6 13 181 7 0.0 6 7 182 5 0.0 6 5 183 5 0.0 6 5 184 5 0.0 6 5 185 13 0.0 6 12 1 186 11 0.0 6 11 187 4 0.0 6 4 188 11 0.0 6 11 189 9 0.0 6 8 0 0 0 1 190 3 0.0 6 2 0 0 1 191 2 0.0 6 2 192 3 0.0 6 3 194 8 0.0 6 7 1 195 5 0.0 6 5 196 5 0.0 6 4 1 197 5 0.0 6 5 198 3 0.0 6 3 199 1 0.0 6 1 200 5 0.0 6 5 201 5 0.0 6 5 202 4 0.0 6 4 203 5 0.0 6 5 204 3 0.0 6 3 205 3 0.0 6 3 206 4 0.0 6 4 207 4 0.0 6 4 208 3 0.0 6 3 209 4 0.0 6 4 210 13 0.0 6 12 0 0 0 0 1 211 3 0.0 6 3 212 2 0.0 6 2 213 2 0.0 6 2 214 1 0.0 6 1 215 2 0.0 6 2 218 1 0.0 6 1 221 1 0.0 6 1 222 2 0.0 6 2 224 2 0.0 6 2 225 1 0.0 6 1 226 1 0.0 6 1 228 1 0.0 6 1 230 2 0.0 6 2 231 1 0.0 6 1 234 1 0.0 6 1 236 1 0.0 6 1 239 1 0.0 6 1 250 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_15_114_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_15_341_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_15_114_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_15_341_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.39 s (680 µs/read; 0.09 M reads/minute). === Summary === Total read pairs processed: 576 Read 1 with adapter: 576 (100.0%) Read 2 with adapter: 574 (99.7%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 2 (0.3%) Pairs written (passing filters): 574 (99.7%) Total basepairs processed: 287,367 bp Read 1: 163,227 bp Read 2: 124,140 bp Total written (filtered): 36,918 bp (12.8%) Read 1: 18,503 bp Read 2: 18,415 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 574 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.7% G: 0.2% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 20 1 0.0 6 1 21 1 0.0 6 1 83 1 0.0 6 1 97 1 0.0 6 1 103 1 0.0 6 1 111 1 0.0 6 1 132 1 0.0 6 1 134 1 0.0 6 1 135 2 0.0 6 2 140 1 0.0 6 1 142 1 0.0 6 1 145 1 0.0 6 1 148 1 0.0 6 1 149 1 0.0 6 1 151 1 0.0 6 1 155 1 0.0 6 1 156 1 0.0 6 1 157 2 0.0 6 2 158 2 0.0 6 2 164 1 0.0 6 1 165 1 0.0 6 1 167 1 0.0 6 0 1 169 4 0.0 6 4 173 1 0.0 6 1 175 1 0.0 6 1 176 1 0.0 6 1 179 1 0.0 6 1 181 2 0.0 6 2 182 2 0.0 6 2 183 1 0.0 6 1 185 2 0.0 6 2 187 1 0.0 6 1 189 1 0.0 6 1 190 1 0.0 6 1 191 1 0.0 6 1 192 2 0.0 6 2 193 3 0.0 6 3 196 2 0.0 6 2 197 3 0.0 6 3 198 2 0.0 6 2 199 2 0.0 6 2 200 3 0.0 6 3 201 1 0.0 6 1 202 2 0.0 6 2 203 2 0.0 6 2 204 3 0.0 6 3 205 3 0.0 6 3 206 3 0.0 6 3 207 4 0.0 6 4 208 4 0.0 6 4 209 1 0.0 6 1 210 2 0.0 6 2 211 2 0.0 6 2 213 4 0.0 6 4 214 2 0.0 6 2 216 2 0.0 6 2 217 5 0.0 6 4 1 218 2 0.0 6 2 219 1 0.0 6 1 220 3 0.0 6 3 222 2 0.0 6 2 223 4 0.0 6 4 225 8 0.0 6 8 226 2 0.0 6 2 227 1 0.0 6 1 229 2 0.0 6 2 230 3 0.0 6 3 231 2 0.0 6 2 232 1 0.0 6 1 233 3 0.0 6 3 234 1 0.0 6 1 235 1 0.0 6 1 236 1 0.0 6 1 237 4 0.0 6 4 238 4 0.0 6 4 239 5 0.0 6 5 240 3 0.0 6 3 241 5 0.0 6 5 242 4 0.0 6 4 243 3 0.0 6 3 244 3 0.0 6 3 245 4 0.0 6 4 246 4 0.0 6 4 248 7 0.0 6 6 0 1 249 2 0.0 6 2 250 2 0.0 6 2 251 8 0.0 6 7 1 252 8 0.0 6 8 253 3 0.0 6 2 1 254 2 0.0 6 2 255 2 0.0 6 2 256 2 0.0 6 2 257 4 0.0 6 4 258 6 0.0 6 6 259 3 0.0 6 2 1 260 4 0.0 6 4 261 6 0.0 6 5 1 262 5 0.0 6 5 263 6 0.0 6 5 1 264 7 0.0 6 7 266 5 0.0 6 4 1 267 3 0.0 6 3 268 6 0.0 6 6 269 14 0.0 6 14 271 3 0.0 6 3 272 7 0.0 6 7 273 11 0.0 6 9 1 1 274 15 0.0 6 14 1 275 7 0.0 6 7 276 16 0.0 6 16 277 15 0.0 6 15 278 96 0.0 6 90 4 2 279 122 0.0 6 119 3 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 83 1 0.0 6 1 87 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 573 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.8% G: 0.2% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 9.0 0 1 1 20 3 0.0 6 3 22 1 0.0 6 1 30 1 0.0 6 1 35 1 0.0 6 1 56 1 0.0 6 1 62 1 0.0 6 1 63 1 0.0 6 1 64 1 0.0 6 1 67 2 0.0 6 2 76 1 0.0 6 0 1 84 1 0.0 6 1 87 1 0.0 6 0 1 89 1 0.0 6 1 94 2 0.0 6 1 1 95 1 0.0 6 1 98 1 0.0 6 1 100 3 0.0 6 3 103 1 0.0 6 1 104 1 0.0 6 1 105 1 0.0 6 1 107 1 0.0 6 1 110 1 0.0 6 1 117 1 0.0 6 1 119 2 0.0 6 2 120 4 0.0 6 4 121 1 0.0 6 1 125 2 0.0 6 2 126 1 0.0 6 1 128 1 0.0 6 1 130 1 0.0 6 0 1 131 1 0.0 6 1 133 1 0.0 6 1 134 2 0.0 6 2 135 5 0.0 6 4 1 136 1 0.0 6 1 137 1 0.0 6 1 140 1 0.0 6 1 141 1 0.0 6 1 142 1 0.0 6 1 143 3 0.0 6 3 144 4 0.0 6 4 145 3 0.0 6 3 146 2 0.0 6 2 147 1 0.0 6 1 148 2 0.0 6 2 149 5 0.0 6 5 150 5 0.0 6 5 151 3 0.0 6 3 152 1 0.0 6 1 154 3 0.0 6 3 155 2 0.0 6 2 156 1 0.0 6 1 157 4 0.0 6 4 159 2 0.0 6 1 1 160 3 0.0 6 3 161 6 0.0 6 6 162 2 0.0 6 2 163 3 0.0 6 3 164 4 0.0 6 3 1 165 5 0.0 6 5 166 6 0.0 6 5 0 0 0 0 1 167 3 0.0 6 3 168 5 0.0 6 5 169 1 0.0 6 1 170 3 0.0 6 3 171 7 0.0 6 7 172 2 0.0 6 2 173 6 0.0 6 5 1 174 7 0.0 6 6 0 0 0 1 175 8 0.0 6 7 0 1 176 4 0.0 6 4 177 6 0.0 6 6 178 7 0.0 6 6 1 179 4 0.0 6 4 180 5 0.0 6 4 0 1 181 6 0.0 6 6 182 2 0.0 6 2 183 8 0.0 6 8 184 3 0.0 6 3 185 7 0.0 6 6 1 186 17 0.0 6 15 2 187 8 0.0 6 8 188 8 0.0 6 8 189 9 0.0 6 9 190 14 0.0 6 13 1 191 5 0.0 6 5 192 4 0.0 6 4 193 4 0.0 6 4 194 15 0.0 6 15 195 17 0.0 6 16 1 196 14 0.0 6 13 1 197 9 0.0 6 8 0 1 198 16 0.0 6 14 2 199 18 0.0 6 16 2 200 11 0.0 6 10 1 201 16 0.0 6 16 202 8 0.0 6 8 203 12 0.0 6 11 1 204 4 0.0 6 4 205 6 0.0 6 6 206 5 0.0 6 5 207 3 0.0 6 3 208 2 0.0 6 2 209 5 0.0 6 5 210 6 0.0 6 6 211 3 0.0 6 3 212 3 0.0 6 3 214 6 0.0 6 6 215 6 0.0 6 5 1 216 9 0.0 6 8 1 217 5 0.0 6 5 218 3 0.0 6 3 219 6 0.0 6 6 220 1 0.0 6 1 221 4 0.0 6 4 222 6 0.0 6 6 223 7 0.0 6 7 224 5 0.0 6 5 225 4 0.0 6 4 226 4 0.0 6 4 227 2 0.0 6 2 228 2 0.0 6 2 229 5 0.0 6 5 230 5 0.0 6 5 231 4 0.0 6 4 232 5 0.0 6 5 233 2 0.0 6 2 235 1 0.0 6 1 238 1 0.0 6 1 240 1 0.0 6 1 241 7 0.0 6 7 242 1 0.0 6 1 243 1 0.0 6 1 246 4 0.0 6 4 247 1 0.0 6 1 248 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 79 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_16_115_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_16_342_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_16_115_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_16_342_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.36 s (33 µs/read; 1.82 M reads/minute). === Summary === Total read pairs processed: 71,486 Read 1 with adapter: 71,476 (100.0%) Read 2 with adapter: 71,468 (100.0%) == Read fate breakdown == Pairs that were too short: 11 (0.0%) Pairs discarded as untrimmed: 28 (0.0%) Pairs written (passing filters): 71,447 (99.9%) Total basepairs processed: 33,393,029 bp Read 1: 18,195,379 bp Read 2: 15,197,650 bp Total written (filtered): 4,582,748 bp (13.7%) Read 1: 2,374,152 bp Read 2: 2,208,596 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 70002 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1117.0 0 0 1 4 6 279.2 1 2 4 6 2 17.5 1 0 0 2 7 2 4.4 2 2 8 1 1.1 2 1 13 1 0.0 3 1 14 2 0.0 4 2 15 3 0.0 4 3 16 5 0.0 4 5 19 3 0.0 5 1 2 20 79 0.0 6 74 5 21 27 0.0 6 27 22 35 0.0 6 34 1 23 22 0.0 6 21 1 24 18 0.0 6 15 1 1 0 1 25 11 0.0 6 9 2 26 11 0.0 6 10 1 27 7 0.0 6 7 28 2 0.0 6 1 1 29 9 0.0 6 9 30 1 0.0 6 1 31 10 0.0 6 10 32 4 0.0 6 4 33 5 0.0 6 5 34 2 0.0 6 2 36 3 0.0 6 3 37 4 0.0 6 4 38 2 0.0 6 2 39 3 0.0 6 3 40 3 0.0 6 3 41 2 0.0 6 2 42 3 0.0 6 3 43 2 0.0 6 1 1 44 2 0.0 6 2 45 1 0.0 6 1 46 4 0.0 6 4 47 5 0.0 6 5 48 3 0.0 6 3 49 5 0.0 6 5 50 1 0.0 6 1 51 2 0.0 6 2 52 9 0.0 6 9 53 6 0.0 6 6 54 2 0.0 6 2 55 1 0.0 6 1 56 6 0.0 6 5 1 57 7 0.0 6 7 58 2 0.0 6 2 59 5 0.0 6 5 60 5 0.0 6 5 61 3 0.0 6 3 62 6 0.0 6 6 63 5 0.0 6 5 64 4 0.0 6 4 65 4 0.0 6 4 66 7 0.0 6 7 67 4 0.0 6 4 68 3 0.0 6 3 69 2 0.0 6 2 70 3 0.0 6 2 0 1 71 2 0.0 6 2 72 4 0.0 6 4 74 2 0.0 6 2 75 3 0.0 6 3 76 4 0.0 6 4 77 3 0.0 6 3 79 3 0.0 6 3 80 1 0.0 6 1 81 6 0.0 6 5 1 82 4 0.0 6 4 83 4 0.0 6 4 84 9 0.0 6 9 85 1 0.0 6 1 86 10 0.0 6 9 1 87 2 0.0 6 1 0 1 88 6 0.0 6 6 89 8 0.0 6 7 1 90 3 0.0 6 3 91 4 0.0 6 3 1 92 4 0.0 6 4 93 2 0.0 6 2 94 2 0.0 6 2 95 4 0.0 6 3 0 0 0 0 1 96 6 0.0 6 6 97 4 0.0 6 4 98 4 0.0 6 4 99 8 0.0 6 7 1 100 5 0.0 6 4 1 101 10 0.0 6 10 102 14 0.0 6 14 103 11 0.0 6 10 1 104 11 0.0 6 10 1 105 9 0.0 6 8 1 106 12 0.0 6 11 0 0 1 107 9 0.0 6 9 108 15 0.0 6 15 109 12 0.0 6 12 110 10 0.0 6 10 111 10 0.0 6 8 2 112 14 0.0 6 13 1 113 8 0.0 6 8 114 14 0.0 6 14 115 9 0.0 6 9 116 13 0.0 6 13 117 20 0.0 6 19 0 0 0 0 0 1 118 13 0.0 6 13 119 12 0.0 6 10 1 0 0 0 1 120 10 0.0 6 10 121 18 0.0 6 18 122 22 0.0 6 22 123 16 0.0 6 16 124 10 0.0 6 10 125 8 0.0 6 8 126 13 0.0 6 13 127 10 0.0 6 10 128 27 0.0 6 26 1 129 13 0.0 6 13 130 15 0.0 6 15 131 23 0.0 6 23 132 34 0.0 6 34 133 33 0.0 6 33 134 31 0.0 6 31 135 25 0.0 6 25 136 39 0.0 6 39 137 45 0.0 6 44 1 138 45 0.0 6 44 1 139 47 0.0 6 46 1 140 28 0.0 6 28 141 121 0.0 6 121 142 106 0.0 6 105 1 143 551 0.0 6 551 144 101 0.0 6 101 145 54 0.0 6 54 146 60 0.0 6 60 147 197 0.0 6 196 1 148 102 0.0 6 102 149 174 0.0 6 171 1 1 0 1 150 1035 0.0 6 1031 4 151 188 0.0 6 186 1 1 152 152 0.0 6 152 153 106 0.0 6 106 154 377 0.0 6 374 3 155 1126 0.0 6 1125 1 156 623 0.0 6 623 157 111 0.0 6 111 158 69 0.0 6 69 159 90 0.0 6 89 1 160 89 0.0 6 88 1 161 54 0.0 6 54 162 79 0.0 6 78 1 163 127 0.0 6 126 0 0 1 164 66 0.0 6 65 0 0 1 165 68 0.0 6 68 166 77 0.0 6 75 2 167 100 0.0 6 99 1 168 169 0.0 6 168 0 0 0 0 0 1 169 295 0.0 6 295 170 1430 0.0 6 1426 3 1 171 1365 0.0 6 1363 1 0 0 0 1 172 1902 0.0 6 1898 4 173 249 0.0 6 248 1 174 119 0.0 6 119 175 370 0.0 6 369 1 176 205 0.0 6 203 2 177 57 0.0 6 57 178 64 0.0 6 64 179 101 0.0 6 96 5 180 270 0.0 6 268 1 0 1 181 233 0.0 6 229 3 0 1 182 1902 0.0 6 1902 183 390 0.0 6 386 4 184 77 0.0 6 77 185 75 0.0 6 74 0 0 0 1 186 105 0.0 6 103 2 187 119 0.0 6 118 1 188 133 0.0 6 129 3 1 189 166 0.0 6 164 2 190 203 0.0 6 203 191 381 0.0 6 380 1 192 2741 0.0 6 2732 9 193 720 0.0 6 718 2 194 331 0.0 6 329 2 195 522 0.0 6 522 196 1461 0.0 6 1459 2 197 369 0.0 6 364 4 0 1 198 161 0.0 6 158 3 199 173 0.0 6 173 200 229 0.0 6 227 2 201 159 0.0 6 155 3 1 202 177 0.0 6 177 203 221 0.0 6 219 2 204 278 0.0 6 274 4 205 409 0.0 6 403 5 0 1 206 1089 0.0 6 1084 5 207 557 0.0 6 554 3 208 1091 0.0 6 1084 7 209 1598 0.0 6 1593 5 210 466 0.0 6 459 7 211 826 0.0 6 818 8 212 229 0.0 6 224 5 213 161 0.0 6 158 3 214 295 0.0 6 289 6 215 158 0.0 6 155 3 216 396 0.0 6 390 6 217 191 0.0 6 189 1 0 1 218 294 0.0 6 291 3 219 223 0.0 6 219 4 220 241 0.0 6 239 2 221 235 0.0 6 233 2 222 388 0.0 6 384 4 223 401 0.0 6 390 9 1 1 224 458 0.0 6 451 7 225 620 0.0 6 616 4 226 792 0.0 6 789 3 227 707 0.0 6 701 5 1 228 1490 0.0 6 1483 6 1 229 268 0.0 6 263 5 230 178 0.0 6 174 4 231 236 0.0 6 233 2 1 232 273 0.0 6 267 6 233 159 0.0 6 153 6 234 242 0.0 6 238 4 235 169 0.0 6 169 236 182 0.0 6 175 7 237 264 0.0 6 259 5 238 205 0.0 6 201 4 239 366 0.0 6 360 6 240 502 0.0 6 495 7 241 292 0.0 6 289 3 242 391 0.0 6 386 3 1 0 0 1 243 605 0.0 6 602 2 0 0 0 1 244 1251 0.0 6 1248 1 2 245 87 0.0 6 87 246 100 0.0 6 93 7 247 176 0.0 6 170 6 248 133 0.0 6 132 1 249 114 0.0 6 108 6 250 237 0.0 6 229 7 1 251 180 0.0 6 170 10 252 600 0.0 6 582 17 1 253 189 0.0 6 185 2 2 254 124 0.0 6 123 1 255 174 0.0 6 167 7 256 299 0.0 6 284 14 1 257 404 0.0 6 390 14 258 400 0.0 6 378 22 259 702 0.0 6 676 26 260 240 0.0 6 224 16 261 408 0.0 6 398 10 262 206 0.0 6 198 8 263 432 0.0 6 421 10 1 264 198 0.0 6 195 3 265 140 0.0 6 132 8 266 331 0.0 6 316 14 1 267 365 0.0 6 354 10 1 268 146 0.0 6 137 9 269 403 0.0 6 381 22 270 162 0.0 6 155 7 271 275 0.0 6 267 8 272 137 0.0 6 129 8 273 221 0.0 6 214 7 274 226 0.0 6 209 17 275 374 0.0 6 349 24 0 1 276 1267 0.0 6 1232 35 277 1040 0.0 6 1008 28 3 1 278 784 0.0 6 681 99 3 0 0 1 279 14636 0.0 6 14284 344 7 0 1 280 7 0.0 6 1 3 2 1 281 5 0.0 6 3 2 284 1 0.0 6 1 288 1 0.0 6 1 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1474 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 3 0.0 6 3 72 4 0.0 6 4 73 12 0.0 6 12 74 21 0.0 6 21 76 1 0.0 6 1 78 5 0.0 6 5 79 1 0.0 6 1 80 15 0.0 6 15 81 39 0.0 6 39 82 6 0.0 6 6 83 5 0.0 6 5 84 5 0.0 6 5 85 21 0.0 6 21 86 38 0.0 6 38 87 22 0.0 6 22 99 1 0.0 6 1 100 17 0.0 6 17 101 60 0.0 6 60 102 65 0.0 6 65 103 53 0.0 6 53 105 3 0.0 6 3 106 14 0.0 6 14 107 1 0.0 6 1 108 1 0.0 6 1 110 4 0.0 6 4 111 9 0.0 6 9 112 29 0.0 6 29 113 69 0.0 6 69 114 5 0.0 6 5 122 34 0.0 6 34 123 108 0.0 6 107 1 124 12 0.0 6 12 125 3 0.0 6 3 126 22 0.0 6 22 127 59 0.0 6 59 128 3 0.0 6 3 129 1 0.0 6 1 130 1 0.0 6 1 131 7 0.0 6 7 132 2 0.0 6 2 133 1 0.0 6 1 135 3 0.0 6 3 136 15 0.0 6 15 137 29 0.0 6 29 138 11 0.0 6 11 139 46 0.0 6 46 140 96 0.0 6 96 141 5 0.0 6 5 154 1 0.0 6 1 155 3 0.0 6 3 156 7 0.0 6 6 1 157 3 0.0 6 3 158 44 0.0 6 43 1 159 145 0.0 6 144 1 160 8 0.0 6 8 172 2 0.0 6 2 173 4 0.0 6 4 174 47 0.0 6 47 175 161 0.0 6 160 1 176 5 0.0 6 5 181 6 0.0 6 6 182 7 0.0 6 7 184 1 0.0 6 1 185 4 0.0 6 4 186 4 0.0 6 4 195 1 0.0 6 1 196 2 0.0 6 2 204 1 0.0 6 1 217 1 0.0 6 1 225 4 0.0 6 4 231 1 0.0 6 1 246 3 0.0 6 3 247 1 0.0 6 1 252 5 0.0 6 5 253 11 0.0 6 11 254 2 0.0 6 2 284 1 0.0 6 1 297 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 69974 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 69.8 1 0 1 7 1 4.4 2 0 1 10 1 0.1 3 1 11 3 0.0 3 3 13 9 0.0 3 7 2 14 4 0.0 4 4 15 1 0.0 4 0 1 16 5 0.0 4 5 17 3 0.0 5 2 1 18 4 0.0 5 4 19 4 0.0 5 3 1 20 41 0.0 6 39 2 21 30 0.0 6 29 1 22 22 0.0 6 21 1 23 20 0.0 6 18 2 24 12 0.0 6 12 25 10 0.0 6 9 1 26 7 0.0 6 5 2 27 8 0.0 6 8 28 8 0.0 6 5 1 1 0 1 29 11 0.0 6 10 0 1 30 13 0.0 6 13 31 9 0.0 6 8 1 32 4 0.0 6 4 33 5 0.0 6 5 34 2 0.0 6 2 35 9 0.0 6 8 1 36 6 0.0 6 5 1 37 4 0.0 6 4 38 5 0.0 6 5 39 7 0.0 6 7 40 5 0.0 6 3 2 41 4 0.0 6 4 42 4 0.0 6 3 0 0 1 43 9 0.0 6 8 1 44 8 0.0 6 7 1 45 8 0.0 6 7 1 46 12 0.0 6 10 2 47 7 0.0 6 7 48 7 0.0 6 6 0 0 0 0 1 49 7 0.0 6 7 50 11 0.0 6 10 1 51 2 0.0 6 2 52 7 0.0 6 6 1 53 9 0.0 6 7 2 54 15 0.0 6 13 0 1 0 0 1 55 13 0.0 6 13 56 11 0.0 6 9 2 57 6 0.0 6 5 1 58 6 0.0 6 4 2 59 8 0.0 6 7 1 60 11 0.0 6 10 0 1 61 7 0.0 6 5 2 62 8 0.0 6 7 0 0 0 0 1 63 3 0.0 6 2 0 0 0 1 64 14 0.0 6 13 1 65 9 0.0 6 8 1 66 18 0.0 6 17 1 67 15 0.0 6 13 2 68 35 0.0 6 33 2 69 125 0.0 6 121 3 0 1 70 38 0.0 6 35 2 1 71 66 0.0 6 64 2 72 24 0.0 6 22 2 73 31 0.0 6 29 2 74 14 0.0 6 13 1 75 13 0.0 6 12 1 76 20 0.0 6 19 1 77 21 0.0 6 19 2 78 13 0.0 6 12 1 79 10 0.0 6 10 80 6 0.0 6 5 1 81 16 0.0 6 15 1 82 12 0.0 6 12 83 23 0.0 6 23 84 23 0.0 6 22 1 85 54 0.0 6 50 4 86 25 0.0 6 24 1 87 18 0.0 6 16 2 88 12 0.0 6 12 89 20 0.0 6 18 2 90 18 0.0 6 17 1 91 29 0.0 6 27 2 92 17 0.0 6 16 1 93 39 0.0 6 37 2 94 44 0.0 6 42 2 95 38 0.0 6 38 96 24 0.0 6 24 97 32 0.0 6 32 98 20 0.0 6 20 99 25 0.0 6 24 0 0 1 100 38 0.0 6 37 1 101 31 0.0 6 29 2 102 27 0.0 6 25 2 103 33 0.0 6 32 1 104 46 0.0 6 43 2 1 105 52 0.0 6 48 4 106 58 0.0 6 58 107 30 0.0 6 30 108 43 0.0 6 41 2 109 42 0.0 6 40 1 0 0 0 1 110 38 0.0 6 36 2 111 45 0.0 6 44 1 112 50 0.0 6 49 1 113 37 0.0 6 37 114 54 0.0 6 53 1 115 56 0.0 6 55 1 116 48 0.0 6 47 1 117 39 0.0 6 38 1 118 41 0.0 6 39 2 119 66 0.0 6 64 1 0 1 120 75 0.0 6 72 3 121 51 0.0 6 51 122 56 0.0 6 53 3 123 72 0.0 6 70 2 124 56 0.0 6 56 125 83 0.0 6 81 2 126 65 0.0 6 64 0 1 127 102 0.0 6 99 3 128 79 0.0 6 76 3 129 97 0.0 6 95 2 130 105 0.0 6 103 2 131 107 0.0 6 106 1 132 137 0.0 6 131 5 0 0 0 0 1 133 116 0.0 6 115 1 134 103 0.0 6 102 1 135 189 0.0 6 186 2 1 136 182 0.0 6 179 2 0 0 0 1 137 228 0.0 6 226 2 138 277 0.0 6 272 3 1 0 1 139 207 0.0 6 206 1 140 313 0.0 6 309 3 1 141 316 0.0 6 313 2 1 142 541 0.0 6 537 4 143 343 0.0 6 340 3 144 275 0.0 6 275 145 273 0.0 6 270 3 146 457 0.0 6 453 4 147 376 0.0 6 375 0 0 0 1 148 420 0.0 6 417 3 149 1057 0.0 6 1050 6 1 150 581 0.0 6 576 4 1 151 581 0.0 6 577 4 152 772 0.0 6 762 8 0 0 2 153 851 0.0 6 848 3 154 1355 0.0 6 1348 7 155 722 0.0 6 718 4 156 383 0.0 6 381 2 157 502 0.0 6 496 6 158 435 0.0 6 428 4 1 0 2 159 334 0.0 6 330 4 160 549 0.0 6 546 3 161 445 0.0 6 441 4 162 466 0.0 6 462 3 0 0 1 163 525 0.0 6 521 4 164 698 0.0 6 691 6 0 0 1 165 775 0.0 6 768 5 1 1 166 702 0.0 6 693 8 0 0 1 167 680 0.0 6 670 10 168 1090 0.0 6 1084 6 169 1279 0.0 6 1274 5 170 1369 0.0 6 1363 6 171 1246 0.0 6 1240 6 172 574 0.0 6 570 4 173 1355 0.0 6 1347 8 174 959 0.0 6 947 12 175 761 0.0 6 749 9 2 0 0 1 176 708 0.0 6 700 8 177 772 0.0 6 757 13 0 0 2 178 1036 0.0 6 1021 14 0 0 1 179 608 0.0 6 603 4 0 0 0 0 1 180 836 0.0 6 825 11 181 787 0.0 6 779 8 182 438 0.0 6 434 4 183 453 0.0 6 448 5 184 513 0.0 6 509 3 0 1 185 779 0.0 6 771 7 0 1 186 921 0.0 6 906 13 1 0 1 187 977 0.0 6 956 17 1 1 1 0 1 188 1026 0.0 6 1015 9 0 0 1 0 1 189 1167 0.0 6 1153 14 190 1114 0.0 6 1091 21 2 191 1296 0.0 6 1270 25 0 1 192 689 0.0 6 678 11 193 886 0.0 6 862 24 194 608 0.0 6 594 14 195 655 0.0 6 638 16 0 0 1 196 781 0.0 6 758 23 197 387 0.0 6 374 12 1 198 1035 0.0 6 1001 33 0 0 1 199 400 0.0 6 386 14 200 695 0.0 6 672 23 201 798 0.0 6 778 19 1 202 788 0.0 6 768 18 1 0 1 203 605 0.0 6 592 11 2 204 752 0.0 6 727 24 0 0 1 205 619 0.0 6 596 21 1 0 1 206 602 0.0 6 587 13 0 0 2 207 573 0.0 6 555 18 208 1039 0.0 6 1014 23 1 1 209 365 0.0 6 359 6 210 562 0.0 6 540 21 0 0 1 211 691 0.0 6 669 22 212 468 0.0 6 453 15 213 199 0.0 6 193 5 1 214 406 0.0 6 388 18 215 500 0.0 6 476 24 216 862 0.0 6 830 29 0 0 3 217 436 0.0 6 420 15 1 218 350 0.0 6 342 8 219 387 0.0 6 380 7 220 215 0.0 6 210 5 221 276 0.0 6 273 2 0 0 1 222 332 0.0 6 324 8 223 260 0.0 6 251 8 0 0 1 224 239 0.0 6 230 6 1 1 1 225 260 0.0 6 251 7 2 226 467 0.0 6 448 18 0 1 227 855 0.0 6 838 17 228 441 0.0 6 422 16 2 0 1 229 714 0.0 6 687 25 0 0 1 1 230 1937 0.0 6 1867 67 0 0 3 231 448 0.0 6 422 23 0 0 3 232 846 0.0 6 815 30 0 0 1 233 85 0.0 6 80 4 0 0 0 1 234 466 0.0 6 449 15 0 0 2 235 211 0.0 6 198 11 0 0 1 1 236 216 0.0 6 202 13 1 237 241 0.0 6 228 13 238 140 0.0 6 137 3 239 17 0.0 6 17 240 63 0.0 6 59 4 241 121 0.0 6 116 5 242 61 0.0 6 61 243 46 0.0 6 43 3 244 71 0.0 6 70 1 245 250 0.0 6 240 8 0 0 2 246 184 0.0 6 175 9 247 101 0.0 6 100 1 248 68 0.0 6 63 5 249 163 0.0 6 156 6 0 0 1 250 60 0.0 6 57 3 251 194 0.0 6 188 5 1 252 79 0.0 6 76 3 253 110 0.0 6 105 5 254 35 0.0 6 31 4 255 28 0.0 6 27 1 256 16 0.0 6 15 1 257 1 0.0 6 1 262 1 0.0 6 1 265 2 0.0 6 2 275 1 0.0 6 1 276 1 0.0 6 1 279 2 0.0 6 2 280 17 0.0 6 17 281 23 0.0 6 20 3 282 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1494 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 1 0.0 6 0 0 1 47 1 0.0 6 0 1 51 2 0.0 6 2 69 1 0.0 6 1 70 6 0.0 6 6 71 11 0.0 6 11 72 15 0.0 6 15 74 2 0.0 6 2 75 1 0.0 6 1 76 7 0.0 6 7 78 7 0.0 6 7 79 52 0.0 6 52 80 4 0.0 6 4 81 5 0.0 6 5 82 6 0.0 6 5 0 0 1 83 20 0.0 6 20 84 50 0.0 6 50 85 20 0.0 6 20 87 1 0.0 6 0 0 0 0 1 88 1 0.0 6 1 91 1 0.0 6 1 92 1 0.0 6 1 96 2 0.0 6 2 97 2 0.0 6 2 98 20 0.0 6 20 99 73 0.0 6 73 100 62 0.0 6 62 101 69 0.0 6 68 1 102 5 0.0 6 4 1 103 1 0.0 6 1 104 15 0.0 6 15 105 2 0.0 6 1 0 0 0 1 107 1 0.0 6 1 108 1 0.0 6 1 109 11 0.0 6 11 110 19 0.0 6 19 111 79 0.0 6 79 112 5 0.0 6 5 118 1 0.0 6 1 119 7 0.0 6 7 120 24 0.0 6 24 121 118 0.0 6 118 122 21 0.0 6 21 123 2 0.0 6 1 1 124 18 0.0 6 18 125 58 0.0 6 58 126 3 0.0 6 3 127 2 0.0 6 2 129 7 0.0 6 7 130 1 0.0 6 1 132 3 0.0 6 3 133 1 0.0 6 0 1 134 6 0.0 6 6 135 36 0.0 6 35 1 136 3 0.0 6 3 137 54 0.0 6 54 138 99 0.0 6 99 139 3 0.0 6 3 142 1 0.0 6 0 0 0 0 1 151 1 0.0 6 1 153 3 0.0 6 2 1 154 13 0.0 6 13 155 2 0.0 6 2 156 29 0.0 6 29 157 140 0.0 6 140 158 7 0.0 6 7 160 1 0.0 6 0 0 0 0 1 169 1 0.0 6 1 170 2 0.0 6 2 171 5 0.0 6 4 1 172 42 0.0 6 42 173 168 0.0 6 168 174 5 0.0 6 4 1 177 1 0.0 6 1 178 1 0.0 6 1 179 2 0.0 6 2 180 6 0.0 6 6 182 2 0.0 6 2 183 1 0.0 6 1 184 2 0.0 6 2 186 1 0.0 6 0 0 0 1 199 1 0.0 6 1 219 1 0.0 6 1 222 1 0.0 6 1 223 1 0.0 6 1 250 3 0.0 6 3 251 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_17_116_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_17_343_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_17_116_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_17_343_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.63 s (29 µs/read; 2.10 M reads/minute). === Summary === Total read pairs processed: 91,916 Read 1 with adapter: 91,895 (100.0%) Read 2 with adapter: 91,878 (100.0%) == Read fate breakdown == Pairs that were too short: 25 (0.0%) Pairs discarded as untrimmed: 58 (0.1%) Pairs written (passing filters): 91,833 (99.9%) Total basepairs processed: 37,036,471 bp Read 1: 20,001,750 bp Read 2: 17,034,721 bp Total written (filtered): 5,895,526 bp (15.9%) Read 1: 3,080,670 bp Read 2: 2,814,856 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 89373 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 1436.2 0 1 3 4 7 359.0 1 4 3 5 1 89.8 1 1 6 4 22.4 1 0 0 4 7 5 5.6 2 1 0 4 8 1 1.4 2 1 9 2 0.4 2 2 10 2 0.1 3 1 1 13 4 0.0 3 4 14 2 0.0 4 2 15 2 0.0 4 1 0 1 16 2 0.0 4 1 1 17 6 0.0 5 6 18 1 0.0 5 1 19 1 0.0 5 0 1 20 149 0.0 6 146 3 21 67 0.0 6 67 22 66 0.0 6 63 2 0 1 23 61 0.0 6 60 1 24 43 0.0 6 41 1 0 1 25 30 0.0 6 30 26 32 0.0 6 31 1 27 21 0.0 6 20 1 28 28 0.0 6 28 29 15 0.0 6 14 1 30 19 0.0 6 17 2 31 20 0.0 6 19 0 0 0 0 1 32 20 0.0 6 19 1 33 12 0.0 6 11 1 34 13 0.0 6 13 35 10 0.0 6 9 1 36 10 0.0 6 9 1 37 12 0.0 6 11 1 38 18 0.0 6 16 1 0 0 0 0 1 39 11 0.0 6 11 40 7 0.0 6 7 41 16 0.0 6 15 1 42 11 0.0 6 9 1 1 43 7 0.0 6 7 44 7 0.0 6 5 1 0 0 1 45 5 0.0 6 5 46 10 0.0 6 9 1 47 12 0.0 6 12 48 7 0.0 6 6 1 49 11 0.0 6 11 50 8 0.0 6 6 2 51 15 0.0 6 14 1 52 11 0.0 6 10 1 53 6 0.0 6 6 54 5 0.0 6 4 1 55 5 0.0 6 4 1 56 5 0.0 6 5 57 7 0.0 6 7 58 11 0.0 6 11 59 8 0.0 6 8 60 9 0.0 6 9 61 7 0.0 6 7 62 3 0.0 6 3 63 4 0.0 6 4 64 8 0.0 6 8 65 3 0.0 6 3 66 15 0.0 6 15 67 7 0.0 6 7 68 8 0.0 6 7 0 0 0 0 0 1 69 13 0.0 6 13 70 16 0.0 6 13 3 71 21 0.0 6 19 2 72 12 0.0 6 9 2 0 1 73 18 0.0 6 18 74 15 0.0 6 14 0 0 0 0 1 75 20 0.0 6 20 76 14 0.0 6 14 77 9 0.0 6 8 1 78 16 0.0 6 14 1 0 0 0 0 1 79 11 0.0 6 11 80 14 0.0 6 14 81 16 0.0 6 16 82 15 0.0 6 15 83 15 0.0 6 15 84 26 0.0 6 25 1 85 20 0.0 6 20 86 3 0.0 6 3 87 17 0.0 6 17 88 9 0.0 6 8 1 89 10 0.0 6 10 90 9 0.0 6 8 1 91 12 0.0 6 12 92 10 0.0 6 10 93 11 0.0 6 10 0 1 94 15 0.0 6 14 1 95 15 0.0 6 15 96 19 0.0 6 19 97 14 0.0 6 13 1 98 6 0.0 6 6 99 13 0.0 6 12 1 100 16 0.0 6 16 101 16 0.0 6 16 102 22 0.0 6 21 1 103 24 0.0 6 23 1 104 28 0.0 6 26 1 0 0 0 1 105 34 0.0 6 34 106 24 0.0 6 23 1 107 24 0.0 6 23 1 108 44 0.0 6 43 1 109 23 0.0 6 23 110 43 0.0 6 42 1 111 47 0.0 6 46 1 112 52 0.0 6 52 113 42 0.0 6 41 1 114 45 0.0 6 44 1 115 44 0.0 6 41 0 0 0 0 3 116 61 0.0 6 61 117 58 0.0 6 57 0 0 0 0 1 118 55 0.0 6 54 0 0 0 0 1 119 37 0.0 6 37 120 48 0.0 6 48 121 38 0.0 6 38 122 58 0.0 6 58 123 52 0.0 6 52 124 45 0.0 6 43 1 0 0 0 0 1 125 52 0.0 6 50 1 0 0 0 0 1 126 55 0.0 6 55 127 48 0.0 6 48 128 54 0.0 6 53 0 1 129 66 0.0 6 66 130 54 0.0 6 53 1 131 84 0.0 6 83 1 132 58 0.0 6 58 133 64 0.0 6 64 134 84 0.0 6 83 1 135 87 0.0 6 86 1 136 90 0.0 6 90 137 84 0.0 6 83 1 138 104 0.0 6 103 1 139 92 0.0 6 92 140 408 0.0 6 406 1 1 141 564 0.0 6 563 1 142 657 0.0 6 656 1 143 714 0.0 6 713 1 144 598 0.0 6 597 1 145 913 0.0 6 911 1 0 0 0 1 146 1401 0.0 6 1399 1 0 0 0 1 147 1571 0.0 6 1567 3 1 148 1713 0.0 6 1709 4 149 1797 0.0 6 1795 1 0 0 0 0 1 150 3074 0.0 6 3071 3 151 1025 0.0 6 1023 2 152 728 0.0 6 727 1 153 706 0.0 6 704 0 0 0 0 1 1 154 625 0.0 6 621 4 155 706 0.0 6 704 2 156 1317 0.0 6 1317 157 863 0.0 6 861 2 158 846 0.0 6 845 1 159 1187 0.0 6 1185 2 160 1123 0.0 6 1118 1 0 0 0 0 4 161 1035 0.0 6 1028 7 162 1048 0.0 6 1046 2 163 599 0.0 6 599 164 1024 0.0 6 1019 4 0 0 1 165 751 0.0 6 750 0 0 0 1 166 851 0.0 6 850 1 167 1154 0.0 6 1149 4 0 1 168 818 0.0 6 817 1 169 948 0.0 6 948 170 976 0.0 6 975 1 171 623 0.0 6 621 2 172 590 0.0 6 590 173 446 0.0 6 446 174 873 0.0 6 871 0 0 1 0 1 175 683 0.0 6 681 1 0 0 0 1 176 534 0.0 6 533 1 177 541 0.0 6 539 2 178 762 0.0 6 758 4 179 617 0.0 6 613 3 0 1 180 749 0.0 6 748 1 181 928 0.0 6 927 1 182 510 0.0 6 508 1 0 0 1 183 666 0.0 6 664 2 184 423 0.0 6 420 2 1 185 684 0.0 6 682 2 186 928 0.0 6 927 1 187 562 0.0 6 560 2 188 488 0.0 6 484 3 0 0 0 0 1 189 773 0.0 6 772 1 190 587 0.0 6 584 3 191 634 0.0 6 633 1 192 678 0.0 6 674 4 193 761 0.0 6 759 2 194 792 0.0 6 788 4 195 985 0.0 6 982 2 1 196 869 0.0 6 867 1 0 0 0 0 1 197 995 0.0 6 990 5 198 1374 0.0 6 1370 4 199 858 0.0 6 852 6 200 1056 0.0 6 1053 3 201 1126 0.0 6 1121 4 1 202 966 0.0 6 964 1 0 0 0 1 203 862 0.0 6 857 4 0 0 1 204 771 0.0 6 768 3 205 1178 0.0 6 1172 6 206 994 0.0 6 987 7 207 849 0.0 6 847 2 208 945 0.0 6 941 4 209 731 0.0 6 729 2 210 639 0.0 6 637 1 0 1 211 769 0.0 6 763 4 1 1 212 833 0.0 6 829 4 213 759 0.0 6 754 4 1 214 773 0.0 6 769 2 1 0 1 215 1068 0.0 6 1066 2 216 655 0.0 6 648 6 0 0 0 1 217 704 0.0 6 702 0 1 1 218 846 0.0 6 840 6 219 726 0.0 6 722 2 0 1 1 220 621 0.0 6 619 1 0 1 221 586 0.0 6 583 3 222 591 0.0 6 590 1 223 539 0.0 6 535 3 0 1 224 540 0.0 6 538 2 225 663 0.0 6 660 3 226 784 0.0 6 782 1 0 0 0 0 1 227 655 0.0 6 652 2 0 0 1 228 452 0.0 6 451 1 229 429 0.0 6 428 1 230 429 0.0 6 427 1 1 231 438 0.0 6 434 4 232 403 0.0 6 403 233 351 0.0 6 349 0 0 1 0 1 234 317 0.0 6 316 1 235 270 0.0 6 269 0 0 0 0 0 1 236 277 0.0 6 275 1 0 0 1 237 349 0.0 6 345 2 0 0 1 0 1 238 354 0.0 6 354 239 282 0.0 6 280 2 240 289 0.0 6 286 3 241 306 0.0 6 304 1 0 0 0 1 242 262 0.0 6 260 2 243 330 0.0 6 325 3 0 2 244 230 0.0 6 229 0 1 245 192 0.0 6 189 2 1 246 167 0.0 6 167 247 181 0.0 6 180 1 248 224 0.0 6 223 1 249 174 0.0 6 174 250 161 0.0 6 161 251 142 0.0 6 137 5 252 132 0.0 6 130 1 0 1 253 135 0.0 6 132 3 254 127 0.0 6 126 1 255 136 0.0 6 135 1 256 102 0.0 6 101 1 257 117 0.0 6 117 258 118 0.0 6 118 259 119 0.0 6 119 260 119 0.0 6 119 261 85 0.0 6 85 262 89 0.0 6 89 263 82 0.0 6 82 264 76 0.0 6 76 265 70 0.0 6 69 1 266 69 0.0 6 69 267 80 0.0 6 80 268 66 0.0 6 66 269 57 0.0 6 57 270 68 0.0 6 67 1 271 63 0.0 6 63 272 49 0.0 6 48 1 273 60 0.0 6 60 274 82 0.0 6 82 275 104 0.0 6 103 1 276 115 0.0 6 114 1 277 152 0.0 6 152 278 543 0.0 6 537 4 0 0 0 1 1 279 1028 0.0 6 1020 7 0 0 0 0 1 280 9 0.0 6 9 281 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2522 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 5 0.0 6 5 71 7 0.0 6 7 72 13 0.0 6 13 73 13 0.0 6 13 74 32 0.0 6 32 75 16 0.0 6 16 76 22 0.0 6 22 77 48 0.0 6 47 1 78 57 0.0 6 57 79 61 0.0 6 61 80 46 0.0 6 46 81 76 0.0 6 76 82 101 0.0 6 101 83 32 0.0 6 32 84 31 0.0 6 31 85 21 0.0 6 21 86 19 0.0 6 19 87 27 0.0 6 26 1 88 41 0.0 6 41 89 36 0.0 6 36 90 35 0.0 6 35 91 36 0.0 6 36 92 28 0.0 6 28 93 29 0.0 6 29 94 22 0.0 6 22 95 15 0.0 6 14 1 96 22 0.0 6 20 2 97 17 0.0 6 17 98 28 0.0 6 28 99 38 0.0 6 38 100 16 0.0 6 16 101 35 0.0 6 35 102 32 0.0 6 32 103 18 0.0 6 18 104 12 0.0 6 12 105 15 0.0 6 15 106 35 0.0 6 35 107 17 0.0 6 16 0 0 0 0 0 1 108 11 0.0 6 11 109 17 0.0 6 17 110 27 0.0 6 27 111 18 0.0 6 18 112 15 0.0 6 15 113 25 0.0 6 25 114 10 0.0 6 10 115 10 0.0 6 10 116 14 0.0 6 14 117 19 0.0 6 19 118 22 0.0 6 22 119 7 0.0 6 7 120 18 0.0 6 18 121 16 0.0 6 16 122 8 0.0 6 8 123 11 0.0 6 11 124 9 0.0 6 9 125 15 0.0 6 15 126 16 0.0 6 16 127 10 0.0 6 10 128 10 0.0 6 10 129 18 0.0 6 18 130 21 0.0 6 21 131 16 0.0 6 16 132 25 0.0 6 25 133 24 0.0 6 24 134 40 0.0 6 40 135 21 0.0 6 20 1 136 19 0.0 6 19 137 18 0.0 6 18 138 11 0.0 6 11 139 21 0.0 6 21 140 12 0.0 6 12 141 16 0.0 6 16 142 16 0.0 6 16 143 18 0.0 6 18 144 21 0.0 6 21 145 16 0.0 6 16 146 20 0.0 6 20 147 19 0.0 6 19 148 6 0.0 6 6 149 13 0.0 6 13 150 19 0.0 6 19 151 15 0.0 6 15 152 2 0.0 6 2 153 17 0.0 6 17 154 16 0.0 6 16 155 9 0.0 6 9 156 4 0.0 6 4 157 14 0.0 6 14 158 25 0.0 6 25 159 18 0.0 6 18 160 9 0.0 6 9 161 9 0.0 6 9 162 15 0.0 6 15 163 19 0.0 6 19 164 17 0.0 6 17 165 14 0.0 6 14 166 6 0.0 6 6 167 3 0.0 6 3 168 9 0.0 6 8 1 169 11 0.0 6 11 170 9 0.0 6 9 171 4 0.0 6 4 172 13 0.0 6 13 173 11 0.0 6 11 174 12 0.0 6 12 175 10 0.0 6 10 176 11 0.0 6 11 177 9 0.0 6 9 178 3 0.0 6 3 179 1 0.0 6 1 180 6 0.0 6 6 181 4 0.0 6 4 182 12 0.0 6 12 183 3 0.0 6 3 184 3 0.0 6 3 185 12 0.0 6 12 186 7 0.0 6 7 187 6 0.0 6 6 188 5 0.0 6 5 189 10 0.0 6 10 190 3 0.0 6 3 191 11 0.0 6 11 192 22 0.0 6 22 193 6 0.0 6 6 194 3 0.0 6 3 195 4 0.0 6 4 197 2 0.0 6 2 198 5 0.0 6 5 199 6 0.0 6 6 200 7 0.0 6 7 201 9 0.0 6 9 202 4 0.0 6 4 203 9 0.0 6 9 204 2 0.0 6 2 205 3 0.0 6 3 206 5 0.0 6 5 207 1 0.0 6 1 208 2 0.0 6 1 1 209 3 0.0 6 3 210 6 0.0 6 6 211 6 0.0 6 6 212 3 0.0 6 3 213 7 0.0 6 7 214 9 0.0 6 9 215 5 0.0 6 5 216 4 0.0 6 4 217 4 0.0 6 4 218 10 0.0 6 10 220 3 0.0 6 3 221 2 0.0 6 2 223 2 0.0 6 2 224 1 0.0 6 1 225 2 0.0 6 2 226 2 0.0 6 2 228 6 0.0 6 6 229 1 0.0 6 1 230 3 0.0 6 3 231 1 0.0 6 1 232 6 0.0 6 6 233 6 0.0 6 6 234 3 0.0 6 3 235 1 0.0 6 1 238 1 0.0 6 1 239 6 0.0 6 6 240 2 0.0 6 2 241 8 0.0 6 8 242 3 0.0 6 3 243 2 0.0 6 2 245 1 0.0 6 1 246 1 0.0 6 1 247 1 0.0 6 1 248 2 0.0 6 2 249 8 0.0 6 8 250 1 0.0 6 1 252 4 0.0 6 3 1 254 2 0.0 6 2 258 2 0.0 6 2 259 1 0.0 6 1 260 2 0.0 6 2 263 1 0.0 6 1 266 3 0.0 6 3 269 1 0.0 6 1 270 1 0.0 6 1 275 1 0.0 6 1 276 1 0.0 6 1 277 1 0.0 6 1 278 2 0.0 6 2 279 1 0.0 6 1 282 3 0.0 6 3 285 1 0.0 6 1 290 1 0.0 6 1 292 1 0.0 6 1 300 1 0.0 6 1 308 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 88699 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1436.2 0 1 7 2 5.6 2 2 10 1 0.1 3 1 11 4 0.0 3 4 12 9 0.0 3 9 13 7 0.0 3 4 3 14 7 0.0 4 7 15 1 0.0 4 1 16 5 0.0 4 5 17 21 0.0 5 17 3 1 18 7 0.0 5 5 2 19 54 0.0 5 50 2 1 1 20 110 0.0 6 108 2 21 54 0.0 6 51 3 22 55 0.0 6 51 4 23 28 0.0 6 24 3 0 1 24 28 0.0 6 26 2 25 22 0.0 6 21 1 26 12 0.0 6 12 27 20 0.0 6 19 1 28 18 0.0 6 15 2 1 29 18 0.0 6 15 3 30 15 0.0 6 15 31 20 0.0 6 17 3 32 16 0.0 6 13 2 1 33 13 0.0 6 11 2 34 15 0.0 6 11 3 0 1 35 14 0.0 6 12 2 36 16 0.0 6 14 2 37 9 0.0 6 9 38 26 0.0 6 24 1 1 39 10 0.0 6 8 1 0 0 0 0 1 40 12 0.0 6 12 41 8 0.0 6 8 42 15 0.0 6 14 1 43 12 0.0 6 11 1 44 18 0.0 6 17 1 45 17 0.0 6 14 1 1 0 0 0 1 46 16 0.0 6 15 0 1 47 16 0.0 6 14 1 0 0 1 48 22 0.0 6 21 1 49 9 0.0 6 9 50 22 0.0 6 22 51 23 0.0 6 19 3 0 1 52 19 0.0 6 14 4 1 53 13 0.0 6 12 1 54 29 0.0 6 27 2 55 28 0.0 6 27 1 56 18 0.0 6 16 1 0 0 1 57 15 0.0 6 12 3 58 18 0.0 6 18 59 26 0.0 6 22 4 60 23 0.0 6 23 61 27 0.0 6 26 1 62 26 0.0 6 24 0 1 1 63 37 0.0 6 33 4 64 19 0.0 6 19 65 35 0.0 6 34 1 66 39 0.0 6 36 2 1 67 42 0.0 6 38 2 1 0 1 68 37 0.0 6 35 1 0 1 69 54 0.0 6 53 1 70 175 0.0 6 174 0 0 0 0 1 71 60 0.0 6 56 3 0 0 0 1 72 83 0.0 6 80 3 73 44 0.0 6 43 1 74 61 0.0 6 60 1 75 61 0.0 6 59 0 2 76 48 0.0 6 45 0 1 1 0 0 1 77 56 0.0 6 55 1 78 53 0.0 6 52 1 79 52 0.0 6 49 3 80 29 0.0 6 26 3 81 36 0.0 6 35 0 0 0 0 1 82 56 0.0 6 54 2 83 57 0.0 6 52 4 1 84 55 0.0 6 50 5 85 52 0.0 6 46 6 86 86 0.0 6 82 3 1 87 63 0.0 6 61 2 88 42 0.0 6 42 89 55 0.0 6 54 1 90 52 0.0 6 48 4 91 58 0.0 6 54 4 92 65 0.0 6 64 1 93 64 0.0 6 61 2 1 94 75 0.0 6 74 0 0 1 95 78 0.0 6 72 2 2 0 1 0 1 96 108 0.0 6 102 4 1 0 0 0 1 97 64 0.0 6 61 2 0 0 0 1 98 89 0.0 6 84 5 99 113 0.0 6 110 3 100 87 0.0 6 87 101 83 0.0 6 81 2 102 84 0.0 6 81 1 1 0 0 1 103 94 0.0 6 91 3 104 103 0.0 6 96 4 0 1 0 0 2 105 112 0.0 6 109 3 106 139 0.0 6 136 3 107 191 0.0 6 189 2 108 106 0.0 6 102 3 0 1 109 103 0.0 6 102 1 110 126 0.0 6 125 1 111 110 0.0 6 105 4 0 0 1 112 151 0.0 6 146 3 1 0 0 1 113 138 0.0 6 135 2 1 114 174 0.0 6 173 1 115 175 0.0 6 173 2 116 197 0.0 6 190 6 0 1 117 186 0.0 6 183 3 118 176 0.0 6 170 5 1 119 175 0.0 6 173 2 120 215 0.0 6 209 4 0 1 1 121 258 0.0 6 256 2 122 235 0.0 6 229 6 123 197 0.0 6 197 124 215 0.0 6 211 2 1 1 125 243 0.0 6 239 3 1 126 272 0.0 6 269 3 127 325 0.0 6 321 3 0 1 128 347 0.0 6 340 6 0 0 0 0 1 129 343 0.0 6 337 5 0 1 130 376 0.0 6 372 4 131 453 0.0 6 443 8 1 0 0 0 1 132 422 0.0 6 419 3 133 482 0.0 6 475 5 1 0 0 0 1 134 527 0.0 6 517 8 1 1 135 500 0.0 6 492 7 0 0 1 136 565 0.0 6 559 2 2 1 0 0 1 137 643 0.0 6 630 10 2 0 0 0 1 138 617 0.0 6 609 6 0 0 1 1 139 644 0.0 6 634 8 0 0 1 0 1 140 862 0.0 6 856 5 0 0 0 0 1 141 931 0.0 6 923 8 142 1096 0.0 6 1087 9 143 1222 0.0 6 1212 7 0 2 0 1 144 1271 0.0 6 1258 8 0 0 1 1 3 145 1716 0.0 6 1704 10 0 0 0 0 2 146 1814 0.0 6 1807 7 147 2134 0.0 6 2123 10 0 0 1 148 2162 0.0 6 2152 7 2 0 0 1 149 2371 0.0 6 2357 14 150 3063 0.0 6 3042 21 151 1862 0.0 6 1847 13 0 1 0 1 152 1746 0.0 6 1728 15 0 0 0 3 153 2114 0.0 6 2100 12 1 0 0 1 154 1629 0.0 6 1615 12 0 0 1 0 1 155 1961 0.0 6 1948 12 1 156 2295 0.0 6 2281 12 0 1 0 1 157 1627 0.0 6 1608 14 2 0 1 1 1 158 1791 0.0 6 1778 11 1 1 159 1729 0.0 6 1720 9 160 1707 0.0 6 1698 8 0 0 0 0 1 161 1729 0.0 6 1715 13 0 0 1 162 1450 0.0 6 1445 5 163 1341 0.0 6 1331 10 164 1474 0.0 6 1463 10 1 165 1088 0.0 6 1078 8 1 0 0 0 1 166 1316 0.0 6 1305 7 2 1 1 167 1247 0.0 6 1240 7 168 1064 0.0 6 1060 3 0 1 169 1415 0.0 6 1410 5 170 1130 0.0 6 1123 7 171 1343 0.0 6 1337 5 1 172 1090 0.0 6 1081 8 0 0 1 173 1056 0.0 6 1045 11 174 1226 0.0 6 1218 7 1 175 1143 0.0 6 1135 7 0 1 176 1111 0.0 6 1104 6 0 1 177 1066 0.0 6 1059 7 178 1026 0.0 6 1020 3 2 1 179 1065 0.0 6 1060 4 0 0 1 180 856 0.0 6 851 5 181 929 0.0 6 926 3 182 602 0.0 6 600 1 1 183 655 0.0 6 651 4 184 577 0.0 6 572 4 0 1 185 555 0.0 6 554 0 1 186 844 0.0 6 838 6 187 982 0.0 6 977 5 188 866 0.0 6 860 5 0 0 1 189 668 0.0 6 662 3 1 1 0 0 1 190 589 0.0 6 589 191 506 0.0 6 503 2 0 0 0 0 1 192 468 0.0 6 465 3 193 432 0.0 6 426 4 0 1 1 194 433 0.0 6 430 3 195 329 0.0 6 328 1 196 292 0.0 6 291 1 197 261 0.0 6 260 1 198 236 0.0 6 235 1 199 339 0.0 6 337 1 0 0 0 0 1 200 307 0.0 6 305 1 1 201 294 0.0 6 294 202 215 0.0 6 213 2 203 168 0.0 6 168 204 172 0.0 6 171 1 205 159 0.0 6 158 1 206 159 0.0 6 156 3 207 111 0.0 6 109 2 208 106 0.0 6 106 209 111 0.0 6 109 2 210 106 0.0 6 105 1 211 78 0.0 6 78 212 83 0.0 6 82 1 213 67 0.0 6 66 1 214 64 0.0 6 63 1 215 70 0.0 6 69 1 216 72 0.0 6 71 1 217 74 0.0 6 73 0 0 0 0 0 1 218 41 0.0 6 41 219 37 0.0 6 37 220 45 0.0 6 44 1 221 40 0.0 6 40 222 26 0.0 6 26 223 21 0.0 6 21 224 30 0.0 6 30 225 31 0.0 6 30 1 226 36 0.0 6 36 227 15 0.0 6 15 228 11 0.0 6 11 229 19 0.0 6 19 230 11 0.0 6 11 231 6 0.0 6 6 232 7 0.0 6 7 233 3 0.0 6 3 234 2 0.0 6 2 235 3 0.0 6 3 236 1 0.0 6 1 237 3 0.0 6 3 238 1 0.0 6 1 239 1 0.0 6 1 240 2 0.0 6 2 241 1 0.0 6 1 243 1 0.0 6 1 244 3 0.0 6 3 247 1 0.0 6 1 248 2 0.0 6 2 249 1 0.0 6 1 251 2 0.0 6 2 252 1 0.0 6 1 255 1 0.0 6 1 279 1 0.0 6 1 280 1 0.0 6 1 281 8 0.0 6 8 282 54 0.0 6 53 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 3179 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 5 0.0 6 0 5 50 3 0.0 6 3 68 2 0.0 6 2 69 14 0.0 6 14 70 21 0.0 6 21 71 25 0.0 6 25 72 25 0.0 6 25 73 14 0.0 6 14 74 39 0.0 6 39 75 49 0.0 6 48 0 0 0 1 76 49 0.0 6 49 77 79 0.0 6 79 78 91 0.0 6 91 79 151 0.0 6 151 80 39 0.0 6 39 81 29 0.0 6 28 0 0 0 1 82 16 0.0 6 16 83 26 0.0 6 26 84 33 0.0 6 33 85 60 0.0 6 60 86 30 0.0 6 28 1 0 0 1 87 34 0.0 6 34 88 62 0.0 6 62 89 37 0.0 6 37 90 303 0.0 6 302 1 91 32 0.0 6 32 92 23 0.0 6 22 1 93 187 0.0 6 187 94 21 0.0 6 21 95 39 0.0 6 39 96 49 0.0 6 49 97 35 0.0 6 34 0 0 0 1 98 34 0.0 6 34 99 29 0.0 6 28 0 0 0 1 100 25 0.0 6 24 0 0 0 1 101 13 0.0 6 12 0 0 1 102 12 0.0 6 12 103 37 0.0 6 37 104 25 0.0 6 25 105 18 0.0 6 18 106 16 0.0 6 16 107 31 0.0 6 31 108 24 0.0 6 24 109 18 0.0 6 18 110 27 0.0 6 27 111 17 0.0 6 17 112 19 0.0 6 18 0 0 1 113 12 0.0 6 12 114 11 0.0 6 11 115 29 0.0 6 28 0 0 0 1 116 13 0.0 6 13 117 15 0.0 6 14 1 118 29 0.0 6 29 119 11 0.0 6 11 120 9 0.0 6 9 121 8 0.0 6 8 122 23 0.0 6 23 123 15 0.0 6 15 124 14 0.0 6 14 125 22 0.0 6 22 126 17 0.0 6 17 127 18 0.0 6 18 128 17 0.0 6 16 1 129 41 0.0 6 41 130 38 0.0 6 38 131 43 0.0 6 42 0 0 0 1 132 27 0.0 6 27 133 13 0.0 6 13 134 41 0.0 6 41 135 13 0.0 6 13 136 16 0.0 6 16 137 22 0.0 6 22 138 14 0.0 6 14 139 21 0.0 6 21 140 21 0.0 6 21 141 13 0.0 6 12 0 0 1 142 12 0.0 6 12 143 12 0.0 6 12 144 33 0.0 6 33 145 9 0.0 6 8 0 0 0 1 146 7 0.0 6 7 147 28 0.0 6 28 148 32 0.0 6 32 149 9 0.0 6 9 150 23 0.0 6 23 151 23 0.0 6 23 152 17 0.0 6 17 153 7 0.0 6 7 154 10 0.0 6 10 155 45 0.0 6 45 156 19 0.0 6 19 157 12 0.0 6 12 158 8 0.0 6 8 159 18 0.0 6 18 160 17 0.0 6 17 161 18 0.0 6 18 162 8 0.0 6 6 1 0 0 1 163 5 0.0 6 5 164 5 0.0 6 5 165 2 0.0 6 2 166 13 0.0 6 13 167 12 0.0 6 12 168 4 0.0 6 4 169 10 0.0 6 10 170 11 0.0 6 11 171 8 0.0 6 8 172 17 0.0 6 17 173 6 0.0 6 6 174 9 0.0 6 9 176 12 0.0 6 11 0 0 1 177 11 0.0 6 11 178 5 0.0 6 5 179 14 0.0 6 13 1 180 3 0.0 6 3 181 6 0.0 6 6 182 7 0.0 6 7 183 8 0.0 6 8 184 5 0.0 6 5 185 6 0.0 6 6 186 6 0.0 6 6 188 5 0.0 6 5 189 12 0.0 6 12 190 3 0.0 6 3 191 4 0.0 6 4 192 2 0.0 6 2 193 2 0.0 6 2 195 1 0.0 6 1 196 3 0.0 6 3 197 5 0.0 6 5 198 2 0.0 6 2 199 2 0.0 6 2 200 12 0.0 6 12 201 1 0.0 6 1 202 3 0.0 6 3 203 1 0.0 6 1 204 2 0.0 6 2 206 1 0.0 6 1 207 1 0.0 6 1 210 1 0.0 6 1 211 1 0.0 6 1 212 2 0.0 6 2 214 1 0.0 6 1 216 1 0.0 6 1 218 1 0.0 6 1 219 1 0.0 6 1 222 1 0.0 6 1 227 1 0.0 6 1 228 1 0.0 6 1 229 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_18_117_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_18_344_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_18_117_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_18_344_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.75 s (34 µs/read; 1.79 M reads/minute). === Summary === Total read pairs processed: 51,983 Read 1 with adapter: 51,978 (100.0%) Read 2 with adapter: 51,954 (99.9%) == Read fate breakdown == Pairs that were too short: 4 (0.0%) Pairs discarded as untrimmed: 33 (0.1%) Pairs written (passing filters): 51,946 (99.9%) Total basepairs processed: 24,833,127 bp Read 1: 13,507,594 bp Read 2: 11,325,533 bp Total written (filtered): 3,391,903 bp (13.7%) Read 1: 1,699,736 bp Read 2: 1,692,167 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 51367 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 812.2 0 0 2 4 1 203.1 1 1 5 1 50.8 1 1 7 2 3.2 2 1 0 1 9 5 0.2 2 4 1 14 1 0.0 4 1 17 3 0.0 5 3 18 1 0.0 5 1 19 1 0.0 5 0 1 20 45 0.0 6 44 1 21 23 0.0 6 23 22 16 0.0 6 16 23 6 0.0 6 6 24 10 0.0 6 9 1 25 7 0.0 6 6 1 26 9 0.0 6 8 1 27 6 0.0 6 6 28 4 0.0 6 4 29 6 0.0 6 6 30 3 0.0 6 3 31 4 0.0 6 3 1 32 1 0.0 6 1 33 2 0.0 6 1 1 34 2 0.0 6 2 35 4 0.0 6 4 36 3 0.0 6 3 37 4 0.0 6 4 38 4 0.0 6 3 1 39 3 0.0 6 3 40 7 0.0 6 7 41 4 0.0 6 4 42 4 0.0 6 4 43 1 0.0 6 1 44 6 0.0 6 5 1 46 3 0.0 6 3 47 1 0.0 6 1 48 2 0.0 6 2 49 2 0.0 6 2 50 1 0.0 6 1 51 3 0.0 6 1 2 52 2 0.0 6 2 53 3 0.0 6 3 54 4 0.0 6 2 2 55 1 0.0 6 1 56 3 0.0 6 3 57 1 0.0 6 1 58 3 0.0 6 3 59 3 0.0 6 3 60 3 0.0 6 3 61 2 0.0 6 2 62 2 0.0 6 2 63 4 0.0 6 3 1 64 3 0.0 6 2 1 65 2 0.0 6 1 1 66 1 0.0 6 1 67 2 0.0 6 2 68 1 0.0 6 1 69 2 0.0 6 2 70 3 0.0 6 3 71 4 0.0 6 4 72 2 0.0 6 2 73 5 0.0 6 5 74 3 0.0 6 3 75 2 0.0 6 2 76 1 0.0 6 1 78 4 0.0 6 3 1 79 2 0.0 6 2 80 1 0.0 6 1 81 1 0.0 6 1 82 2 0.0 6 2 83 5 0.0 6 5 84 3 0.0 6 3 85 1 0.0 6 1 86 5 0.0 6 5 87 5 0.0 6 5 88 5 0.0 6 4 0 0 0 0 1 89 3 0.0 6 3 90 6 0.0 6 3 2 0 0 0 1 91 3 0.0 6 3 92 3 0.0 6 3 93 3 0.0 6 2 1 94 2 0.0 6 2 95 3 0.0 6 3 96 4 0.0 6 4 97 6 0.0 6 6 98 5 0.0 6 4 1 99 10 0.0 6 9 1 100 6 0.0 6 6 101 11 0.0 6 11 102 9 0.0 6 8 1 103 8 0.0 6 8 104 9 0.0 6 9 105 9 0.0 6 9 106 6 0.0 6 6 107 15 0.0 6 14 1 108 18 0.0 6 17 1 109 7 0.0 6 7 110 13 0.0 6 13 111 5 0.0 6 5 112 14 0.0 6 14 113 10 0.0 6 8 2 114 8 0.0 6 8 115 9 0.0 6 8 0 0 0 1 116 5 0.0 6 5 117 7 0.0 6 5 0 1 0 0 0 1 118 5 0.0 6 5 119 7 0.0 6 5 1 1 120 5 0.0 6 5 121 8 0.0 6 8 122 23 0.0 6 23 123 13 0.0 6 13 124 18 0.0 6 18 125 9 0.0 6 9 126 24 0.0 6 23 1 127 29 0.0 6 29 128 27 0.0 6 26 0 1 129 23 0.0 6 22 1 130 13 0.0 6 12 1 131 14 0.0 6 13 1 132 30 0.0 6 30 133 13 0.0 6 13 134 29 0.0 6 29 135 34 0.0 6 34 136 24 0.0 6 24 137 25 0.0 6 24 1 138 27 0.0 6 27 139 40 0.0 6 40 140 41 0.0 6 41 141 53 0.0 6 51 2 142 118 0.0 6 118 143 609 0.0 6 609 144 197 0.0 6 196 1 145 652 0.0 6 650 2 146 407 0.0 6 407 147 458 0.0 6 457 1 148 128 0.0 6 127 1 149 23 0.0 6 23 150 21 0.0 6 20 1 151 20 0.0 6 20 152 18 0.0 6 18 153 19 0.0 6 19 154 23 0.0 6 23 155 44 0.0 6 43 1 156 46 0.0 6 46 157 72 0.0 6 71 1 158 67 0.0 6 66 1 159 124 0.0 6 121 3 160 785 0.0 6 783 2 161 207 0.0 6 206 1 162 67 0.0 6 67 163 110 0.0 6 110 164 77 0.0 6 76 1 165 408 0.0 6 407 1 166 96 0.0 6 95 0 1 167 145 0.0 6 145 168 978 0.0 6 976 2 169 141 0.0 6 141 170 93 0.0 6 92 1 171 531 0.0 6 530 1 172 138 0.0 6 136 1 0 0 0 1 173 92 0.0 6 91 0 0 0 1 174 215 0.0 6 213 2 175 1174 0.0 6 1173 1 176 290 0.0 6 289 1 177 294 0.0 6 288 6 178 2138 0.0 6 2136 2 179 354 0.0 6 354 180 52 0.0 6 50 2 181 31 0.0 6 31 182 20 0.0 6 19 1 183 18 0.0 6 18 184 13 0.0 6 13 185 30 0.0 6 29 1 186 31 0.0 6 31 187 28 0.0 6 27 1 188 30 0.0 6 29 1 189 70 0.0 6 69 1 190 44 0.0 6 42 2 191 33 0.0 6 30 3 192 66 0.0 6 64 2 193 135 0.0 6 133 2 194 95 0.0 6 92 2 0 1 195 188 0.0 6 182 6 196 229 0.0 6 224 5 197 172 0.0 6 168 4 198 248 0.0 6 241 7 199 101 0.0 6 98 3 200 259 0.0 6 248 11 201 178 0.0 6 178 202 101 0.0 6 99 2 203 73 0.0 6 71 2 204 120 0.0 6 116 4 205 192 0.0 6 190 2 206 231 0.0 6 228 2 1 207 100 0.0 6 94 6 208 188 0.0 6 185 3 209 129 0.0 6 126 3 210 198 0.0 6 191 6 1 211 155 0.0 6 149 6 212 179 0.0 6 173 6 213 342 0.0 6 336 5 0 0 1 214 251 0.0 6 240 11 215 223 0.0 6 215 8 216 221 0.0 6 217 4 217 184 0.0 6 183 1 218 176 0.0 6 173 3 219 165 0.0 6 160 4 0 0 0 0 1 220 122 0.0 6 119 3 221 114 0.0 6 110 3 1 222 174 0.0 6 173 1 223 149 0.0 6 146 3 224 430 0.0 6 423 7 225 264 0.0 6 260 4 226 311 0.0 6 306 5 227 235 0.0 6 234 1 228 322 0.0 6 320 2 229 567 0.0 6 563 4 230 235 0.0 6 229 6 231 496 0.0 6 490 6 232 307 0.0 6 304 3 233 333 0.0 6 327 4 2 234 500 0.0 6 491 9 235 238 0.0 6 223 15 236 833 0.0 6 819 14 237 266 0.0 6 258 7 1 238 322 0.0 6 316 6 239 259 0.0 6 252 7 240 311 0.0 6 299 12 241 439 0.0 6 426 12 1 242 661 0.0 6 629 32 243 1024 0.0 6 994 27 2 1 244 411 0.0 6 393 16 1 1 245 742 0.0 6 720 21 0 0 1 246 422 0.0 6 404 18 247 1369 0.0 6 1317 52 248 359 0.0 6 345 13 1 249 275 0.0 6 264 11 250 531 0.0 6 520 11 251 808 0.0 6 795 13 252 306 0.0 6 296 10 253 590 0.0 6 572 18 254 352 0.0 6 345 7 255 346 0.0 6 335 10 1 256 137 0.0 6 130 7 257 155 0.0 6 148 7 258 137 0.0 6 132 5 259 255 0.0 6 242 12 0 1 260 465 0.0 6 449 15 1 261 323 0.0 6 306 17 262 422 0.0 6 411 11 263 675 0.0 6 658 17 264 461 0.0 6 449 11 1 265 647 0.0 6 625 20 2 266 510 0.0 6 497 13 267 358 0.0 6 351 7 268 279 0.0 6 269 10 269 529 0.0 6 518 11 270 335 0.0 6 328 7 271 387 0.0 6 377 10 272 458 0.0 6 445 12 1 273 721 0.0 6 691 30 274 1712 0.0 6 1653 59 275 1249 0.0 6 1217 31 1 276 920 0.0 6 885 34 0 1 277 2759 0.0 6 2700 56 1 2 278 3578 0.0 6 3460 116 1 1 279 716 0.0 6 693 22 1 280 4 0.0 6 3 1 281 3 0.0 6 0 1 1 0 0 1 283 1 0.0 6 0 0 0 1 284 3 0.0 6 3 285 2 0.0 6 2 286 1 0.0 6 1 287 2 0.0 6 2 289 1 0.0 6 1 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 611 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 1 0.0 6 1 71 5 0.0 6 5 72 25 0.0 6 25 73 10 0.0 6 10 74 23 0.0 6 23 75 19 0.0 6 19 76 11 0.0 6 11 77 5 0.0 6 5 85 1 0.0 6 1 86 3 0.0 6 3 87 1 0.0 6 1 88 9 0.0 6 9 89 25 0.0 6 25 90 3 0.0 6 3 92 4 0.0 6 4 93 6 0.0 6 6 94 15 0.0 6 15 95 3 0.0 6 3 96 14 0.0 6 14 97 52 0.0 6 52 98 1 0.0 6 1 99 5 0.0 6 5 100 18 0.0 6 18 101 1 0.0 6 1 102 1 0.0 6 1 103 8 0.0 6 8 104 42 0.0 6 42 105 4 0.0 6 4 106 35 0.0 6 35 107 79 0.0 6 79 108 7 0.0 6 7 115 1 0.0 6 1 130 1 0.0 6 1 135 6 0.0 6 6 156 3 0.0 6 3 157 19 0.0 6 19 158 52 0.0 6 52 159 2 0.0 6 2 162 9 0.0 6 9 163 22 0.0 6 22 164 1 0.0 6 1 165 1 0.0 6 1 166 5 0.0 6 5 167 15 0.0 6 15 168 1 0.0 6 1 218 1 0.0 6 1 219 1 0.0 6 1 221 2 0.0 6 2 222 1 0.0 6 1 223 3 0.0 6 3 230 1 0.0 6 1 239 1 0.0 6 1 240 6 0.0 6 6 241 8 0.0 6 8 244 1 0.0 6 1 252 2 0.0 6 2 253 4 0.0 6 4 256 1 0.0 6 1 263 2 0.0 6 2 264 2 0.0 6 2 282 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 51301 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 812.2 0 0 1 11 1 0.0 3 1 13 4 0.0 3 3 1 14 3 0.0 4 3 15 2 0.0 4 1 1 16 3 0.0 4 3 17 7 0.0 5 5 2 18 8 0.0 5 7 1 19 2 0.0 5 2 20 32 0.0 6 28 4 21 33 0.0 6 33 22 8 0.0 6 8 23 4 0.0 6 4 24 8 0.0 6 7 1 25 16 0.0 6 15 1 26 6 0.0 6 4 1 1 27 3 0.0 6 3 28 3 0.0 6 3 29 6 0.0 6 6 30 3 0.0 6 3 31 6 0.0 6 6 32 4 0.0 6 4 33 5 0.0 6 5 34 2 0.0 6 2 35 3 0.0 6 3 36 4 0.0 6 4 37 5 0.0 6 4 0 1 38 6 0.0 6 5 1 39 4 0.0 6 4 40 1 0.0 6 1 41 4 0.0 6 3 1 42 4 0.0 6 4 43 8 0.0 6 8 44 4 0.0 6 4 45 4 0.0 6 3 1 46 13 0.0 6 12 1 47 8 0.0 6 6 2 48 3 0.0 6 3 49 11 0.0 6 9 2 50 7 0.0 6 7 51 8 0.0 6 8 52 10 0.0 6 9 1 53 9 0.0 6 8 1 54 9 0.0 6 8 1 55 5 0.0 6 5 56 14 0.0 6 12 2 57 9 0.0 6 8 1 58 13 0.0 6 11 1 0 0 1 59 5 0.0 6 4 1 60 6 0.0 6 6 61 1 0.0 6 0 1 62 5 0.0 6 3 1 1 63 7 0.0 6 5 2 64 6 0.0 6 6 65 14 0.0 6 13 1 66 19 0.0 6 17 1 1 67 101 0.0 6 96 4 1 68 23 0.0 6 22 0 1 69 45 0.0 6 45 70 9 0.0 6 9 71 23 0.0 6 22 1 72 20 0.0 6 18 1 0 0 1 73 9 0.0 6 9 74 5 0.0 6 5 75 9 0.0 6 7 1 1 76 16 0.0 6 16 77 11 0.0 6 11 78 15 0.0 6 14 1 79 14 0.0 6 14 80 22 0.0 6 20 2 81 14 0.0 6 13 1 82 22 0.0 6 20 2 83 36 0.0 6 33 3 84 19 0.0 6 18 1 85 17 0.0 6 15 2 86 12 0.0 6 12 87 21 0.0 6 19 1 1 88 12 0.0 6 12 89 14 0.0 6 13 0 1 90 17 0.0 6 14 2 0 1 91 18 0.0 6 18 92 18 0.0 6 17 1 93 21 0.0 6 18 3 94 24 0.0 6 22 2 95 16 0.0 6 16 96 11 0.0 6 9 2 97 14 0.0 6 14 98 16 0.0 6 16 99 12 0.0 6 12 100 19 0.0 6 19 101 11 0.0 6 11 102 25 0.0 6 25 103 32 0.0 6 30 2 104 37 0.0 6 36 1 105 35 0.0 6 33 2 106 15 0.0 6 15 107 23 0.0 6 20 2 1 108 36 0.0 6 34 2 109 20 0.0 6 20 110 26 0.0 6 26 111 40 0.0 6 39 1 112 28 0.0 6 27 1 113 37 0.0 6 35 1 0 0 1 114 39 0.0 6 37 2 115 46 0.0 6 46 116 45 0.0 6 43 1 0 0 1 117 43 0.0 6 43 118 42 0.0 6 40 1 0 0 1 119 47 0.0 6 46 1 120 44 0.0 6 41 2 0 0 1 121 49 0.0 6 46 2 1 122 49 0.0 6 47 2 123 64 0.0 6 61 3 124 68 0.0 6 67 1 125 64 0.0 6 58 6 126 52 0.0 6 50 2 127 50 0.0 6 48 2 128 55 0.0 6 55 129 51 0.0 6 50 1 130 49 0.0 6 47 1 1 131 68 0.0 6 67 1 132 97 0.0 6 92 4 1 133 135 0.0 6 132 2 1 134 139 0.0 6 137 2 135 187 0.0 6 182 4 1 136 166 0.0 6 162 4 137 156 0.0 6 153 2 1 138 246 0.0 6 242 4 139 297 0.0 6 291 4 1 0 1 140 618 0.0 6 615 3 141 379 0.0 6 376 2 0 0 1 142 713 0.0 6 709 4 143 529 0.0 6 522 6 1 144 492 0.0 6 487 5 145 260 0.0 6 260 146 173 0.0 6 172 1 147 121 0.0 6 118 2 1 148 286 0.0 6 282 3 0 0 1 149 370 0.0 6 363 7 150 300 0.0 6 294 5 0 1 151 377 0.0 6 371 6 152 373 0.0 6 363 7 1 1 1 153 447 0.0 6 438 8 0 1 154 334 0.0 6 329 4 1 155 433 0.0 6 421 12 156 466 0.0 6 457 8 1 157 698 0.0 6 693 5 158 403 0.0 6 398 5 159 333 0.0 6 326 7 160 362 0.0 6 356 6 161 301 0.0 6 296 4 0 0 1 162 406 0.0 6 401 5 163 338 0.0 6 327 11 164 440 0.0 6 433 7 165 595 0.0 6 586 9 166 362 0.0 6 358 3 0 0 1 167 517 0.0 6 509 7 0 0 1 168 499 0.0 6 491 8 169 370 0.0 6 365 5 170 507 0.0 6 499 7 0 0 1 171 484 0.0 6 472 10 1 1 172 914 0.0 6 903 11 173 611 0.0 6 597 13 0 0 1 174 855 0.0 6 842 10 0 0 3 175 1087 0.0 6 1070 14 2 0 1 176 589 0.0 6 569 20 177 757 0.0 6 737 20 178 318 0.0 6 303 13 2 179 314 0.0 6 298 14 2 180 562 0.0 6 546 15 0 0 0 1 181 120 0.0 6 116 4 182 205 0.0 6 201 4 183 242 0.0 6 235 6 0 0 1 184 427 0.0 6 412 13 1 1 185 609 0.0 6 587 20 1 1 186 601 0.0 6 586 14 1 187 301 0.0 6 297 4 188 458 0.0 6 442 16 189 377 0.0 6 362 13 1 0 1 190 351 0.0 6 340 9 2 191 495 0.0 6 479 15 1 192 624 0.0 6 610 13 0 0 1 193 230 0.0 6 226 4 194 359 0.0 6 348 11 195 634 0.0 6 606 27 0 0 1 196 456 0.0 6 448 8 197 209 0.0 6 199 10 198 378 0.0 6 370 8 199 361 0.0 6 355 6 200 488 0.0 6 479 8 0 0 1 201 258 0.0 6 249 9 202 321 0.0 6 314 7 203 188 0.0 6 187 1 204 228 0.0 6 219 8 0 1 205 303 0.0 6 294 7 0 1 1 206 273 0.0 6 266 7 207 267 0.0 6 257 8 0 0 2 208 254 0.0 6 250 3 1 209 319 0.0 6 308 10 0 1 210 307 0.0 6 287 20 211 1634 0.0 6 1581 50 1 1 1 212 310 0.0 6 290 17 3 213 753 0.0 6 712 37 2 1 0 0 1 214 2117 0.0 6 2053 61 1 1 1 215 517 0.0 6 497 19 0 1 216 1175 0.0 6 1143 31 1 217 357 0.0 6 344 12 0 0 1 218 374 0.0 6 358 15 0 0 0 1 219 312 0.0 6 296 16 220 278 0.0 6 270 8 221 734 0.0 6 715 18 0 1 222 807 0.0 6 785 22 223 259 0.0 6 251 7 0 0 1 224 492 0.0 6 480 11 1 225 396 0.0 6 387 9 226 421 0.0 6 409 10 1 0 1 227 257 0.0 6 251 6 228 513 0.0 6 489 22 1 0 1 229 738 0.0 6 720 18 230 181 0.0 6 178 3 231 153 0.0 6 146 7 232 164 0.0 6 156 8 233 244 0.0 6 235 8 1 234 470 0.0 6 455 14 0 1 235 445 0.0 6 434 11 236 230 0.0 6 224 6 237 88 0.0 6 83 4 1 238 307 0.0 6 296 11 239 388 0.0 6 371 17 240 369 0.0 6 361 8 241 716 0.0 6 692 24 242 241 0.0 6 235 5 1 243 200 0.0 6 194 6 244 138 0.0 6 133 4 0 0 1 245 54 0.0 6 52 2 246 71 0.0 6 68 3 247 88 0.0 6 82 5 0 0 1 248 217 0.0 6 213 4 249 75 0.0 6 74 0 0 0 1 250 122 0.0 6 117 5 251 91 0.0 6 89 2 252 33 0.0 6 31 2 253 21 0.0 6 20 1 254 9 0.0 6 8 1 255 2 0.0 6 2 256 1 0.0 6 1 259 2 0.0 6 2 263 2 0.0 6 2 264 2 0.0 6 2 265 1 0.0 6 1 278 3 0.0 6 3 279 6 0.0 6 6 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 653 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 3 0.0 6 3 71 5 0.0 6 5 72 22 0.0 6 22 73 5 0.0 6 5 74 21 0.0 6 21 75 16 0.0 6 16 76 21 0.0 6 21 77 5 0.0 6 4 1 87 2 0.0 6 2 88 7 0.0 6 7 89 32 0.0 6 32 90 6 0.0 6 6 91 1 0.0 6 1 92 5 0.0 6 4 0 0 1 93 5 0.0 6 4 0 0 0 1 94 19 0.0 6 19 95 1 0.0 6 1 96 10 0.0 6 10 97 48 0.0 6 48 98 2 0.0 6 2 99 2 0.0 6 2 100 21 0.0 6 21 102 1 0.0 6 1 103 16 0.0 6 16 104 42 0.0 6 41 1 105 1 0.0 6 1 106 20 0.0 6 20 107 115 0.0 6 115 108 7 0.0 6 7 118 4 0.0 6 4 122 1 0.0 6 1 128 1 0.0 6 1 131 1 0.0 6 1 133 1 0.0 6 1 134 3 0.0 6 3 135 8 0.0 6 8 152 1 0.0 6 1 156 3 0.0 6 3 157 10 0.0 6 10 158 59 0.0 6 59 161 2 0.0 6 2 162 7 0.0 6 7 163 37 0.0 6 37 164 2 0.0 6 2 166 7 0.0 6 7 167 28 0.0 6 28 168 1 0.0 6 1 237 1 0.0 6 1 239 1 0.0 6 1 240 4 0.0 6 4 241 7 0.0 6 7 253 3 0.0 6 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_19_118_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_19_345_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_19_118_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_19_345_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.65 s (268 µs/read; 0.22 M reads/minute). === Summary === Total read pairs processed: 2,416 Read 1 with adapter: 2,415 (100.0%) Read 2 with adapter: 2,416 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 1 (0.0%) Pairs written (passing filters): 2,415 (100.0%) Total basepairs processed: 1,110,909 bp Read 1: 620,841 bp Read 2: 490,068 bp Total written (filtered): 151,434 bp (13.6%) Read 1: 78,220 bp Read 2: 73,214 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 2398 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 11 1 0.0 3 1 17 1 0.0 5 1 20 1 0.0 6 1 21 3 0.0 6 3 22 1 0.0 6 1 24 1 0.0 6 1 29 1 0.0 6 1 41 1 0.0 6 1 45 1 0.0 6 1 52 1 0.0 6 1 61 1 0.0 6 1 68 1 0.0 6 1 71 1 0.0 6 1 77 1 0.0 6 1 78 1 0.0 6 1 81 1 0.0 6 1 92 1 0.0 6 1 94 1 0.0 6 1 101 1 0.0 6 1 102 1 0.0 6 0 0 1 107 1 0.0 6 1 111 1 0.0 6 1 114 1 0.0 6 1 117 1 0.0 6 1 120 1 0.0 6 1 122 1 0.0 6 1 123 1 0.0 6 1 124 2 0.0 6 2 127 3 0.0 6 3 128 1 0.0 6 1 129 2 0.0 6 2 130 1 0.0 6 1 131 1 0.0 6 1 132 2 0.0 6 2 135 1 0.0 6 1 136 1 0.0 6 1 137 1 0.0 6 1 138 1 0.0 6 1 139 5 0.0 6 5 140 5 0.0 6 5 141 8 0.0 6 8 142 13 0.0 6 13 143 29 0.0 6 29 144 17 0.0 6 16 1 145 13 0.0 6 13 146 32 0.0 6 32 147 33 0.0 6 33 148 14 0.0 6 14 149 13 0.0 6 13 150 8 0.0 6 8 151 12 0.0 6 12 152 11 0.0 6 11 153 7 0.0 6 7 154 2 0.0 6 2 155 5 0.0 6 5 156 2 0.0 6 2 157 6 0.0 6 6 158 11 0.0 6 11 159 22 0.0 6 22 160 20 0.0 6 20 161 11 0.0 6 11 162 5 0.0 6 5 163 6 0.0 6 6 164 16 0.0 6 16 165 11 0.0 6 11 166 14 0.0 6 14 167 12 0.0 6 11 1 168 13 0.0 6 13 169 7 0.0 6 7 170 7 0.0 6 7 171 8 0.0 6 8 172 15 0.0 6 15 173 3 0.0 6 3 174 4 0.0 6 4 175 4 0.0 6 4 176 8 0.0 6 8 177 16 0.0 6 16 178 8 0.0 6 8 179 16 0.0 6 16 180 6 0.0 6 6 181 3 0.0 6 3 182 5 0.0 6 5 183 12 0.0 6 12 184 13 0.0 6 13 185 9 0.0 6 9 186 10 0.0 6 10 187 7 0.0 6 7 188 4 0.0 6 4 189 2 0.0 6 2 190 9 0.0 6 9 191 6 0.0 6 6 192 7 0.0 6 7 193 8 0.0 6 8 194 11 0.0 6 11 195 18 0.0 6 18 196 18 0.0 6 18 197 11 0.0 6 11 198 9 0.0 6 9 199 10 0.0 6 10 200 10 0.0 6 10 201 14 0.0 6 13 1 202 11 0.0 6 11 203 12 0.0 6 12 204 11 0.0 6 11 205 11 0.0 6 11 206 8 0.0 6 8 207 17 0.0 6 17 208 8 0.0 6 7 1 209 14 0.0 6 14 210 13 0.0 6 13 211 11 0.0 6 11 212 9 0.0 6 9 213 16 0.0 6 16 214 17 0.0 6 17 215 13 0.0 6 12 1 216 11 0.0 6 11 217 12 0.0 6 12 218 16 0.0 6 16 219 10 0.0 6 10 220 10 0.0 6 10 221 8 0.0 6 8 222 11 0.0 6 11 223 6 0.0 6 6 224 6 0.0 6 6 225 13 0.0 6 12 1 226 27 0.0 6 26 1 227 23 0.0 6 23 228 14 0.0 6 14 229 18 0.0 6 18 230 11 0.0 6 11 231 12 0.0 6 12 232 21 0.0 6 21 233 15 0.0 6 14 0 0 1 234 17 0.0 6 17 235 15 0.0 6 15 236 16 0.0 6 16 237 7 0.0 6 7 238 5 0.0 6 5 239 10 0.0 6 10 240 18 0.0 6 18 241 25 0.0 6 25 242 33 0.0 6 33 243 18 0.0 6 18 244 16 0.0 6 16 245 24 0.0 6 24 246 7 0.0 6 7 247 25 0.0 6 24 1 248 11 0.0 6 9 2 249 23 0.0 6 22 1 250 12 0.0 6 12 251 30 0.0 6 29 1 252 14 0.0 6 14 253 15 0.0 6 15 254 24 0.0 6 22 2 255 12 0.0 6 12 256 16 0.0 6 15 1 257 18 0.0 6 18 258 17 0.0 6 15 2 259 41 0.0 6 39 2 260 35 0.0 6 34 1 261 12 0.0 6 12 262 17 0.0 6 17 263 47 0.0 6 45 2 264 10 0.0 6 10 265 33 0.0 6 32 1 266 18 0.0 6 18 267 14 0.0 6 14 268 24 0.0 6 23 1 269 28 0.0 6 27 1 270 11 0.0 6 11 271 15 0.0 6 14 1 272 40 0.0 6 40 273 24 0.0 6 24 274 34 0.0 6 34 275 46 0.0 6 45 1 276 45 0.0 6 43 2 277 94 0.0 6 93 1 278 76 0.0 6 74 2 279 169 0.0 6 163 6 280 3 0.0 6 2 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 17 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 3 0.0 6 3 78 1 0.0 6 1 79 3 0.0 6 3 92 1 0.0 6 1 96 1 0.0 6 1 102 1 0.0 6 1 103 1 0.0 6 1 110 1 0.0 6 1 171 1 0.0 6 1 173 2 0.0 6 2 174 1 0.0 6 1 193 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 2401 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 0.1 2 0 1 12 1 0.0 3 1 13 1 0.0 3 1 16 1 0.0 4 1 17 1 0.0 5 1 20 1 0.0 6 1 21 2 0.0 6 2 23 1 0.0 6 1 24 2 0.0 6 2 26 1 0.0 6 1 27 1 0.0 6 1 28 1 0.0 6 1 30 1 0.0 6 1 31 1 0.0 6 1 34 1 0.0 6 1 38 1 0.0 6 1 39 2 0.0 6 2 40 1 0.0 6 1 44 1 0.0 6 1 46 2 0.0 6 2 53 3 0.0 6 3 55 1 0.0 6 1 56 2 0.0 6 2 57 1 0.0 6 1 59 1 0.0 6 1 60 1 0.0 6 0 1 61 2 0.0 6 2 63 2 0.0 6 2 64 1 0.0 6 1 69 3 0.0 6 3 70 1 0.0 6 1 71 3 0.0 6 3 72 1 0.0 6 1 73 2 0.0 6 2 75 1 0.0 6 1 78 1 0.0 6 1 79 1 0.0 6 1 80 3 0.0 6 3 82 2 0.0 6 2 83 1 0.0 6 1 84 3 0.0 6 3 85 3 0.0 6 3 88 3 0.0 6 3 90 1 0.0 6 1 91 4 0.0 6 4 92 1 0.0 6 1 94 2 0.0 6 2 95 2 0.0 6 2 97 2 0.0 6 2 99 2 0.0 6 2 100 2 0.0 6 2 101 4 0.0 6 4 102 1 0.0 6 1 103 3 0.0 6 2 1 104 1 0.0 6 1 105 3 0.0 6 3 106 1 0.0 6 1 108 2 0.0 6 1 0 0 0 1 109 2 0.0 6 1 0 0 0 0 1 110 1 0.0 6 1 111 1 0.0 6 1 112 2 0.0 6 2 113 3 0.0 6 3 114 1 0.0 6 1 115 3 0.0 6 3 116 4 0.0 6 4 117 3 0.0 6 3 118 4 0.0 6 4 119 3 0.0 6 3 120 5 0.0 6 5 121 3 0.0 6 3 122 8 0.0 6 8 123 4 0.0 6 4 124 8 0.0 6 8 125 6 0.0 6 6 126 6 0.0 6 6 127 6 0.0 6 6 128 3 0.0 6 3 129 8 0.0 6 8 130 5 0.0 6 5 131 12 0.0 6 12 132 11 0.0 6 11 133 4 0.0 6 4 134 15 0.0 6 15 135 13 0.0 6 12 1 136 17 0.0 6 17 137 11 0.0 6 11 138 11 0.0 6 11 139 13 0.0 6 13 140 18 0.0 6 18 141 28 0.0 6 28 142 35 0.0 6 35 143 29 0.0 6 28 1 144 26 0.0 6 26 145 30 0.0 6 29 1 146 37 0.0 6 37 147 19 0.0 6 19 148 14 0.0 6 14 149 14 0.0 6 14 150 35 0.0 6 34 1 151 23 0.0 6 23 152 43 0.0 6 43 153 18 0.0 6 17 1 154 30 0.0 6 30 155 28 0.0 6 28 156 23 0.0 6 23 157 34 0.0 6 32 2 158 27 0.0 6 27 159 32 0.0 6 32 160 24 0.0 6 24 161 17 0.0 6 17 162 16 0.0 6 16 163 18 0.0 6 17 1 164 20 0.0 6 20 165 33 0.0 6 31 2 166 27 0.0 6 26 1 167 13 0.0 6 13 168 26 0.0 6 25 1 169 18 0.0 6 18 170 22 0.0 6 20 1 0 0 1 171 33 0.0 6 33 172 8 0.0 6 7 0 0 0 1 173 12 0.0 6 11 1 174 18 0.0 6 18 175 37 0.0 6 35 2 176 17 0.0 6 16 0 0 0 1 177 28 0.0 6 27 1 178 22 0.0 6 22 179 21 0.0 6 21 180 24 0.0 6 23 1 181 16 0.0 6 16 182 13 0.0 6 13 183 14 0.0 6 14 184 14 0.0 6 13 1 185 18 0.0 6 18 186 50 0.0 6 48 2 187 42 0.0 6 42 188 34 0.0 6 33 1 189 36 0.0 6 34 2 190 45 0.0 6 44 1 191 36 0.0 6 35 1 192 31 0.0 6 31 193 18 0.0 6 17 1 194 29 0.0 6 26 3 195 52 0.0 6 50 1 0 0 1 196 86 0.0 6 82 4 197 15 0.0 6 15 198 61 0.0 6 60 1 199 67 0.0 6 64 3 200 17 0.0 6 14 2 1 201 23 0.0 6 22 1 202 23 0.0 6 22 1 203 12 0.0 6 12 204 12 0.0 6 12 205 5 0.0 6 5 206 11 0.0 6 10 1 207 9 0.0 6 9 208 9 0.0 6 9 209 7 0.0 6 7 210 5 0.0 6 4 1 211 18 0.0 6 18 212 9 0.0 6 9 213 2 0.0 6 2 214 32 0.0 6 30 2 215 6 0.0 6 4 2 216 33 0.0 6 29 4 217 13 0.0 6 13 218 8 0.0 6 8 219 10 0.0 6 10 220 8 0.0 6 8 221 16 0.0 6 15 1 222 23 0.0 6 23 223 29 0.0 6 29 224 3 0.0 6 3 225 11 0.0 6 11 226 5 0.0 6 5 227 2 0.0 6 2 228 4 0.0 6 4 229 4 0.0 6 4 230 4 0.0 6 4 231 6 0.0 6 6 232 1 0.0 6 1 233 6 0.0 6 6 235 4 0.0 6 4 236 4 0.0 6 4 238 1 0.0 6 1 239 2 0.0 6 2 240 3 0.0 6 3 241 2 0.0 6 2 242 4 0.0 6 4 243 4 0.0 6 4 244 1 0.0 6 1 245 1 0.0 6 1 246 2 0.0 6 2 248 1 0.0 6 1 249 2 0.0 6 2 250 1 0.0 6 1 252 1 0.0 6 1 253 3 0.0 6 3 279 1 0.0 6 1 280 2 0.0 6 2 281 9 0.0 6 9 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 15 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 75 2 0.0 6 2 76 2 0.0 6 2 78 2 0.0 6 2 80 1 0.0 6 1 99 1 0.0 6 1 101 1 0.0 6 1 108 1 0.0 6 1 123 1 0.0 6 1 130 2 0.0 6 2 160 1 0.0 6 1 170 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_20_119_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_20_346_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_20_119_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_20_346_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.41 s (63 µs/read; 0.95 M reads/minute). === Summary === Total read pairs processed: 38,014 Read 1 with adapter: 38,013 (100.0%) Read 2 with adapter: 37,976 (99.9%) == Read fate breakdown == Pairs that were too short: 1 (0.0%) Pairs discarded as untrimmed: 39 (0.1%) Pairs written (passing filters): 37,974 (99.9%) Total basepairs processed: 19,050,489 bp Read 1: 10,782,539 bp Read 2: 8,267,950 bp Total written (filtered): 2,429,948 bp (12.8%) Read 1: 1,215,112 bp Read 2: 1,214,836 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 38010 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 37.1 1 1 7 1 2.3 2 0 0 1 14 1 0.0 4 1 15 1 0.0 4 1 20 4 0.0 6 4 21 5 0.0 6 5 22 4 0.0 6 4 24 1 0.0 6 1 25 7 0.0 6 6 1 26 3 0.0 6 3 28 1 0.0 6 0 1 29 2 0.0 6 1 1 30 2 0.0 6 2 31 2 0.0 6 2 32 2 0.0 6 2 34 1 0.0 6 0 1 37 1 0.0 6 1 38 1 0.0 6 1 40 3 0.0 6 2 1 41 1 0.0 6 1 42 4 0.0 6 4 44 1 0.0 6 1 45 3 0.0 6 3 47 2 0.0 6 2 50 2 0.0 6 2 52 1 0.0 6 1 53 2 0.0 6 2 54 1 0.0 6 1 56 4 0.0 6 4 57 1 0.0 6 1 60 2 0.0 6 2 61 3 0.0 6 3 62 1 0.0 6 1 63 1 0.0 6 1 64 3 0.0 6 3 65 2 0.0 6 2 67 2 0.0 6 2 68 2 0.0 6 2 69 1 0.0 6 1 70 1 0.0 6 1 72 3 0.0 6 3 73 1 0.0 6 1 74 1 0.0 6 1 78 2 0.0 6 2 79 1 0.0 6 1 80 1 0.0 6 1 81 1 0.0 6 1 82 1 0.0 6 1 86 6 0.0 6 6 87 2 0.0 6 1 1 89 3 0.0 6 3 90 5 0.0 6 4 1 91 1 0.0 6 0 1 93 2 0.0 6 2 94 1 0.0 6 1 97 2 0.0 6 2 98 8 0.0 6 8 100 3 0.0 6 2 1 101 8 0.0 6 8 102 1 0.0 6 1 103 2 0.0 6 2 104 1 0.0 6 1 105 3 0.0 6 2 1 106 1 0.0 6 1 107 1 0.0 6 1 108 10 0.0 6 9 1 109 1 0.0 6 1 110 1 0.0 6 1 111 5 0.0 6 4 1 112 4 0.0 6 3 0 1 113 3 0.0 6 3 114 2 0.0 6 2 115 2 0.0 6 2 116 3 0.0 6 3 117 5 0.0 6 5 118 2 0.0 6 2 119 5 0.0 6 4 1 120 2 0.0 6 2 121 5 0.0 6 5 122 9 0.0 6 8 0 1 123 4 0.0 6 4 124 8 0.0 6 6 2 125 6 0.0 6 5 1 126 4 0.0 6 4 127 2 0.0 6 2 128 17 0.0 6 17 129 5 0.0 6 5 130 4 0.0 6 4 131 1 0.0 6 1 132 7 0.0 6 7 133 11 0.0 6 11 134 6 0.0 6 6 135 13 0.0 6 12 1 136 7 0.0 6 7 137 23 0.0 6 22 1 138 16 0.0 6 15 1 139 5 0.0 6 5 140 14 0.0 6 14 141 16 0.0 6 16 142 14 0.0 6 14 143 22 0.0 6 21 1 144 7 0.0 6 6 1 145 24 0.0 6 24 146 25 0.0 6 24 1 147 16 0.0 6 15 0 0 1 148 18 0.0 6 16 2 149 27 0.0 6 24 3 150 21 0.0 6 20 1 151 17 0.0 6 17 152 18 0.0 6 17 1 153 5 0.0 6 5 154 11 0.0 6 10 1 155 21 0.0 6 21 156 12 0.0 6 12 157 16 0.0 6 14 2 158 14 0.0 6 13 1 159 29 0.0 6 29 160 27 0.0 6 27 161 20 0.0 6 18 2 162 29 0.0 6 28 1 163 16 0.0 6 15 1 164 14 0.0 6 12 2 165 21 0.0 6 21 166 20 0.0 6 19 0 1 167 15 0.0 6 15 168 27 0.0 6 27 169 26 0.0 6 25 1 170 27 0.0 6 27 171 27 0.0 6 26 1 172 19 0.0 6 19 173 29 0.0 6 28 1 174 19 0.0 6 17 1 1 175 28 0.0 6 27 1 176 30 0.0 6 30 177 34 0.0 6 33 1 178 23 0.0 6 21 2 179 37 0.0 6 36 1 180 25 0.0 6 23 2 181 21 0.0 6 20 0 1 182 56 0.0 6 52 4 183 58 0.0 6 57 1 184 74 0.0 6 72 2 185 97 0.0 6 92 5 186 126 0.0 6 123 3 187 71 0.0 6 68 3 188 55 0.0 6 51 4 189 64 0.0 6 63 1 190 63 0.0 6 62 1 191 74 0.0 6 69 4 1 192 79 0.0 6 74 5 193 81 0.0 6 78 2 1 194 91 0.0 6 89 2 195 121 0.0 6 118 3 196 103 0.0 6 100 3 197 155 0.0 6 152 3 198 163 0.0 6 159 4 199 116 0.0 6 114 2 200 146 0.0 6 144 2 201 129 0.0 6 123 6 202 129 0.0 6 127 2 203 179 0.0 6 172 7 204 97 0.0 6 94 3 205 202 0.0 6 194 8 206 167 0.0 6 165 2 207 196 0.0 6 195 1 208 198 0.0 6 192 6 209 188 0.0 6 183 5 210 146 0.0 6 144 2 211 205 0.0 6 202 3 212 134 0.0 6 132 2 213 174 0.0 6 171 3 214 213 0.0 6 209 4 215 136 0.0 6 134 2 216 205 0.0 6 200 5 217 196 0.0 6 186 10 218 170 0.0 6 168 2 219 130 0.0 6 128 2 220 103 0.0 6 103 221 105 0.0 6 101 4 222 238 0.0 6 236 2 223 110 0.0 6 108 2 224 140 0.0 6 132 7 0 1 225 198 0.0 6 193 5 226 446 0.0 6 440 6 227 316 0.0 6 308 8 228 292 0.0 6 282 10 229 261 0.0 6 256 4 1 230 253 0.0 6 245 8 231 232 0.0 6 228 4 232 262 0.0 6 254 7 0 0 1 233 231 0.0 6 227 4 234 193 0.0 6 191 2 235 88 0.0 6 84 4 236 161 0.0 6 155 6 237 212 0.0 6 209 3 238 253 0.0 6 247 5 1 239 278 0.0 6 269 9 240 365 0.0 6 358 7 241 340 0.0 6 333 7 242 341 0.0 6 336 5 243 263 0.0 6 257 6 244 219 0.0 6 213 6 245 339 0.0 6 334 5 246 201 0.0 6 192 8 1 247 271 0.0 6 267 4 248 182 0.0 6 177 5 249 319 0.0 6 314 5 250 355 0.0 6 349 6 251 302 0.0 6 294 8 252 408 0.0 6 403 5 253 228 0.0 6 223 5 254 469 0.0 6 460 9 255 357 0.0 6 344 13 256 332 0.0 6 322 10 257 387 0.0 6 379 8 258 490 0.0 6 471 19 259 641 0.0 6 629 10 2 260 641 0.0 6 631 10 261 373 0.0 6 367 5 1 262 243 0.0 6 235 8 263 648 0.0 6 634 14 264 228 0.0 6 221 7 265 409 0.0 6 397 11 1 266 317 0.0 6 316 1 267 430 0.0 6 420 10 268 475 0.0 6 462 13 269 571 0.0 6 554 17 270 323 0.0 6 315 8 271 316 0.0 6 308 8 272 613 0.0 6 603 9 0 1 273 592 0.0 6 585 7 274 638 0.0 6 628 10 275 670 0.0 6 655 15 276 1298 0.0 6 1282 16 277 1341 0.0 6 1307 34 278 2975 0.0 6 2882 89 2 1 0 1 279 7475 0.0 6 7281 184 10 280 36 0.0 6 28 7 1 281 5 0.0 6 4 0 1 282 1 0.0 6 0 0 0 1 283 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 3 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 73 1 0.0 6 1 74 1 0.0 6 1 110 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 37973 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 9.3 1 1 10 2 0.0 3 1 0 0 1 11 2 0.0 3 2 12 1 0.0 3 1 13 5 0.0 3 4 1 14 3 0.0 4 2 1 16 5 0.0 4 4 1 17 9 0.0 5 7 1 1 18 7 0.0 5 7 19 6 0.0 5 6 20 25 0.0 6 24 1 21 20 0.0 6 20 22 9 0.0 6 8 1 23 9 0.0 6 7 2 24 17 0.0 6 16 1 25 14 0.0 6 14 26 8 0.0 6 8 27 8 0.0 6 8 28 7 0.0 6 7 29 13 0.0 6 13 30 3 0.0 6 3 31 6 0.0 6 6 32 6 0.0 6 6 33 5 0.0 6 5 34 5 0.0 6 5 35 4 0.0 6 4 36 6 0.0 6 5 0 1 37 8 0.0 6 8 38 3 0.0 6 3 39 6 0.0 6 4 1 1 40 5 0.0 6 5 41 6 0.0 6 6 42 1 0.0 6 1 43 5 0.0 6 4 1 44 9 0.0 6 8 1 45 5 0.0 6 2 1 1 0 1 46 15 0.0 6 11 4 47 5 0.0 6 5 48 10 0.0 6 9 1 49 16 0.0 6 15 1 50 7 0.0 6 6 1 51 3 0.0 6 3 52 19 0.0 6 16 2 1 53 9 0.0 6 9 54 12 0.0 6 12 55 6 0.0 6 5 1 56 13 0.0 6 13 57 10 0.0 6 8 1 1 58 11 0.0 6 11 59 7 0.0 6 7 60 14 0.0 6 11 2 1 61 7 0.0 6 5 2 62 5 0.0 6 4 1 63 5 0.0 6 5 64 7 0.0 6 6 1 65 5 0.0 6 5 66 12 0.0 6 10 1 1 67 72 0.0 6 71 1 68 17 0.0 6 15 2 69 21 0.0 6 20 1 70 6 0.0 6 5 0 1 71 19 0.0 6 18 1 72 20 0.0 6 20 73 6 0.0 6 5 1 74 13 0.0 6 12 1 75 8 0.0 6 7 1 76 16 0.0 6 15 1 77 11 0.0 6 10 1 78 17 0.0 6 17 79 15 0.0 6 11 4 80 8 0.0 6 7 0 1 81 5 0.0 6 5 82 17 0.0 6 16 1 83 33 0.0 6 32 1 84 20 0.0 6 19 1 85 12 0.0 6 10 2 86 9 0.0 6 8 1 87 27 0.0 6 25 2 88 15 0.0 6 12 3 89 12 0.0 6 10 2 90 12 0.0 6 9 3 91 18 0.0 6 17 1 92 9 0.0 6 9 93 29 0.0 6 25 4 94 35 0.0 6 35 95 21 0.0 6 19 2 96 21 0.0 6 19 2 97 15 0.0 6 15 98 20 0.0 6 19 1 99 27 0.0 6 26 1 100 33 0.0 6 33 101 29 0.0 6 26 3 102 28 0.0 6 25 3 103 37 0.0 6 35 2 104 62 0.0 6 60 1 1 105 33 0.0 6 31 2 106 19 0.0 6 18 1 107 24 0.0 6 23 1 108 17 0.0 6 17 109 23 0.0 6 23 110 30 0.0 6 29 1 111 37 0.0 6 33 2 1 0 1 112 21 0.0 6 20 0 1 113 23 0.0 6 22 1 114 42 0.0 6 39 3 115 18 0.0 6 18 116 27 0.0 6 27 117 22 0.0 6 21 1 118 31 0.0 6 31 119 25 0.0 6 23 1 0 0 1 120 44 0.0 6 42 2 121 34 0.0 6 33 0 1 122 47 0.0 6 47 123 43 0.0 6 43 124 62 0.0 6 59 3 125 55 0.0 6 49 5 0 0 1 126 41 0.0 6 40 1 127 48 0.0 6 45 3 128 23 0.0 6 23 129 39 0.0 6 36 3 130 41 0.0 6 38 2 0 0 1 131 86 0.0 6 85 1 132 94 0.0 6 89 5 133 70 0.0 6 68 1 0 1 134 117 0.0 6 113 3 0 0 1 135 176 0.0 6 173 3 136 74 0.0 6 69 4 0 1 137 96 0.0 6 93 2 1 138 56 0.0 6 52 4 139 91 0.0 6 85 5 1 140 135 0.0 6 128 7 141 137 0.0 6 131 5 0 0 1 142 181 0.0 6 175 4 0 0 2 143 153 0.0 6 151 2 144 232 0.0 6 227 5 145 135 0.0 6 133 2 146 106 0.0 6 102 4 147 73 0.0 6 68 5 148 124 0.0 6 116 6 1 0 0 0 1 149 242 0.0 6 230 11 1 150 343 0.0 6 326 16 1 151 101 0.0 6 96 5 152 365 0.0 6 350 15 153 178 0.0 6 168 9 1 154 239 0.0 6 234 5 155 208 0.0 6 197 10 1 156 179 0.0 6 173 6 157 262 0.0 6 252 9 0 0 1 158 254 0.0 6 243 11 159 120 0.0 6 117 3 160 238 0.0 6 230 8 161 131 0.0 6 127 3 0 0 1 162 106 0.0 6 98 7 1 163 222 0.0 6 219 3 164 219 0.0 6 211 7 0 1 165 216 0.0 6 211 5 166 303 0.0 6 293 10 167 161 0.0 6 155 5 1 168 439 0.0 6 432 7 169 161 0.0 6 156 5 170 185 0.0 6 174 11 171 450 0.0 6 436 13 0 1 172 164 0.0 6 160 4 173 238 0.0 6 235 3 174 359 0.0 6 346 11 0 1 0 0 1 175 500 0.0 6 490 10 176 351 0.0 6 339 10 2 177 358 0.0 6 346 12 178 334 0.0 6 319 14 1 179 361 0.0 6 353 7 1 180 365 0.0 6 353 12 181 420 0.0 6 406 12 2 182 101 0.0 6 97 2 0 1 1 183 379 0.0 6 368 11 184 358 0.0 6 348 10 185 426 0.0 6 406 20 186 844 0.0 6 824 18 1 1 187 667 0.0 6 651 14 1 1 188 679 0.0 6 662 16 1 189 669 0.0 6 647 22 190 941 0.0 6 917 24 191 1007 0.0 6 988 17 2 192 498 0.0 6 484 13 0 0 1 193 490 0.0 6 474 14 1 0 1 194 693 0.0 6 672 19 1 0 0 1 195 959 0.0 6 925 34 196 1428 0.0 6 1392 36 197 543 0.0 6 516 26 1 198 1169 0.0 6 1149 19 1 199 1243 0.0 6 1213 28 1 0 1 200 665 0.0 6 651 12 0 0 2 201 772 0.0 6 748 22 1 1 202 489 0.0 6 485 4 203 430 0.0 6 418 9 2 1 204 466 0.0 6 457 9 205 265 0.0 6 255 9 0 0 1 206 242 0.0 6 234 7 0 0 0 1 207 231 0.0 6 225 6 208 396 0.0 6 390 6 209 147 0.0 6 141 6 210 148 0.0 6 141 6 1 211 277 0.0 6 270 6 1 212 242 0.0 6 240 2 213 125 0.0 6 119 4 0 2 214 383 0.0 6 376 7 215 345 0.0 6 339 5 0 0 1 216 517 0.0 6 507 10 217 258 0.0 6 246 11 1 218 259 0.0 6 251 8 219 155 0.0 6 150 5 220 107 0.0 6 101 5 0 0 1 221 199 0.0 6 191 8 222 334 0.0 6 318 16 223 339 0.0 6 327 11 1 224 154 0.0 6 151 3 225 194 0.0 6 187 6 1 226 171 0.0 6 163 8 227 315 0.0 6 311 4 228 268 0.0 6 262 6 229 305 0.0 6 298 7 230 607 0.0 6 594 12 0 0 1 231 230 0.0 6 225 5 232 445 0.0 6 437 8 233 114 0.0 6 114 234 105 0.0 6 100 5 235 64 0.0 6 61 3 236 41 0.0 6 40 1 237 46 0.0 6 44 1 0 0 0 1 238 194 0.0 6 189 5 239 35 0.0 6 35 240 51 0.0 6 51 241 126 0.0 6 122 3 0 0 0 1 242 99 0.0 6 94 4 1 243 76 0.0 6 76 244 43 0.0 6 42 1 245 95 0.0 6 92 3 246 61 0.0 6 60 1 247 41 0.0 6 41 248 46 0.0 6 46 249 9 0.0 6 8 1 250 19 0.0 6 19 251 49 0.0 6 49 252 3 0.0 6 3 253 4 0.0 6 4 254 5 0.0 6 5 255 3 0.0 6 3 256 1 0.0 6 1 261 1 0.0 6 1 277 4 0.0 6 4 278 17 0.0 6 17 279 20 0.0 6 20 280 2 0.0 6 2 281 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 3 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 88 1 0.0 6 1 157 1 0.0 6 1 223 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_01_108_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_01_335_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_01_108_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_01_335_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_02_109_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_02_336_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_02_109_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_02_336_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_03_110_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_03_337_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_03_110_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_03_337_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_04_111_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_04_338_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_04_111_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_04_338_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_05_112_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_05_339_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_05_112_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_05_339_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_06_113_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_06_340_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_06_113_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_06_340_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_15_114_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_15_341_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_15_114_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_15_341_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_16_115_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_16_342_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_16_115_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_16_342_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_17_116_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_17_343_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_17_116_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_17_343_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_18_117_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_18_344_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_18_117_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_18_344_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_19_118_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_19_345_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_19_118_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_19_345_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_20_119_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_10-Rev_20_346_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_20_119_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_10-Rev_20_346_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_01_120_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_01_347_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_01_120_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_01_347_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.96 s (32 µs/read; 1.86 M reads/minute). === Summary === Total read pairs processed: 91,950 Read 1 with adapter: 91,943 (100.0%) Read 2 with adapter: 91,891 (99.9%) == Read fate breakdown == Pairs that were too short: 18 (0.0%) Pairs discarded as untrimmed: 66 (0.1%) Pairs written (passing filters): 91,866 (99.9%) Total basepairs processed: 43,739,604 bp Read 1: 24,890,401 bp Read 2: 18,849,203 bp Total written (filtered): 5,824,413 bp (13.3%) Read 1: 3,056,805 bp Read 2: 2,767,608 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 91368 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1436.7 0 1 4 8 359.2 1 6 2 5 1 89.8 1 1 6 5 22.4 1 1 0 4 7 6 5.6 2 1 0 5 9 3 0.4 2 2 1 12 1 0.0 3 1 15 1 0.0 4 0 1 16 3 0.0 4 3 17 2 0.0 5 1 1 19 3 0.0 5 2 1 20 72 0.0 6 70 2 21 29 0.0 6 28 1 22 29 0.0 6 29 23 14 0.0 6 14 24 5 0.0 6 5 25 17 0.0 6 16 1 26 15 0.0 6 15 27 8 0.0 6 7 1 28 4 0.0 6 4 29 6 0.0 6 6 30 5 0.0 6 5 31 5 0.0 6 4 1 32 3 0.0 6 2 0 1 33 5 0.0 6 5 34 1 0.0 6 1 35 2 0.0 6 2 36 2 0.0 6 1 1 37 3 0.0 6 3 38 10 0.0 6 8 2 39 2 0.0 6 2 40 17 0.0 6 16 1 41 5 0.0 6 5 42 1 0.0 6 1 43 1 0.0 6 1 44 8 0.0 6 8 45 14 0.0 6 13 1 46 8 0.0 6 8 47 3 0.0 6 2 1 48 4 0.0 6 4 49 2 0.0 6 2 50 1 0.0 6 1 51 4 0.0 6 4 52 5 0.0 6 5 53 7 0.0 6 7 55 6 0.0 6 6 56 7 0.0 6 7 57 6 0.0 6 6 58 2 0.0 6 2 59 2 0.0 6 2 60 6 0.0 6 6 61 1 0.0 6 1 62 2 0.0 6 2 63 3 0.0 6 3 64 7 0.0 6 6 1 65 4 0.0 6 4 66 5 0.0 6 5 67 7 0.0 6 6 1 68 4 0.0 6 4 69 1 0.0 6 1 70 9 0.0 6 8 1 71 8 0.0 6 8 72 3 0.0 6 3 73 5 0.0 6 4 1 74 2 0.0 6 2 75 4 0.0 6 4 76 3 0.0 6 3 77 6 0.0 6 6 78 2 0.0 6 2 79 3 0.0 6 3 80 2 0.0 6 2 81 8 0.0 6 8 82 4 0.0 6 3 0 0 0 0 0 1 83 5 0.0 6 5 84 8 0.0 6 8 85 6 0.0 6 6 86 9 0.0 6 9 87 11 0.0 6 11 88 5 0.0 6 5 89 5 0.0 6 5 90 4 0.0 6 4 91 3 0.0 6 2 1 92 1 0.0 6 1 93 8 0.0 6 7 1 94 7 0.0 6 7 95 6 0.0 6 6 96 7 0.0 6 7 97 8 0.0 6 8 98 9 0.0 6 9 99 10 0.0 6 10 100 11 0.0 6 11 101 12 0.0 6 12 102 8 0.0 6 8 103 14 0.0 6 14 104 17 0.0 6 16 0 1 105 10 0.0 6 10 106 10 0.0 6 9 1 107 10 0.0 6 10 108 8 0.0 6 8 109 10 0.0 6 10 110 15 0.0 6 15 111 20 0.0 6 19 1 112 11 0.0 6 10 1 113 23 0.0 6 21 2 114 14 0.0 6 14 115 20 0.0 6 19 0 0 0 0 1 116 10 0.0 6 10 117 19 0.0 6 18 1 118 15 0.0 6 15 119 16 0.0 6 16 120 13 0.0 6 12 1 121 30 0.0 6 28 2 122 28 0.0 6 28 123 18 0.0 6 17 1 124 28 0.0 6 26 2 125 16 0.0 6 16 126 47 0.0 6 46 1 127 48 0.0 6 46 2 128 38 0.0 6 36 2 129 22 0.0 6 21 1 130 31 0.0 6 30 1 131 31 0.0 6 29 2 132 30 0.0 6 29 1 133 24 0.0 6 23 1 134 30 0.0 6 29 1 135 45 0.0 6 43 2 136 53 0.0 6 53 137 43 0.0 6 42 1 138 41 0.0 6 41 139 69 0.0 6 66 3 140 50 0.0 6 49 1 141 153 0.0 6 153 142 307 0.0 6 304 3 143 366 0.0 6 360 3 3 144 239 0.0 6 237 2 145 216 0.0 6 215 1 146 355 0.0 6 352 2 1 147 303 0.0 6 303 148 284 0.0 6 282 2 149 284 0.0 6 284 150 205 0.0 6 203 2 151 236 0.0 6 233 3 152 192 0.0 6 192 153 153 0.0 6 152 1 154 179 0.0 6 179 155 193 0.0 6 192 1 156 156 0.0 6 156 157 125 0.0 6 123 2 158 167 0.0 6 166 1 159 169 0.0 6 164 5 160 180 0.0 6 177 3 161 221 0.0 6 220 1 162 182 0.0 6 180 1 1 163 166 0.0 6 165 1 164 194 0.0 6 193 1 165 128 0.0 6 128 166 155 0.0 6 155 167 204 0.0 6 201 2 1 168 193 0.0 6 189 3 1 169 227 0.0 6 225 2 170 242 0.0 6 239 2 1 171 221 0.0 6 216 4 1 172 181 0.0 6 179 2 173 180 0.0 6 179 1 174 182 0.0 6 179 3 175 203 0.0 6 199 3 1 176 210 0.0 6 206 4 177 209 0.0 6 207 2 178 287 0.0 6 284 3 179 591 0.0 6 586 5 180 348 0.0 6 340 8 181 256 0.0 6 254 1 1 182 234 0.0 6 229 5 183 207 0.0 6 200 6 1 184 266 0.0 6 257 9 185 284 0.0 6 281 3 186 247 0.0 6 244 3 187 248 0.0 6 244 3 0 0 0 1 188 245 0.0 6 241 4 189 230 0.0 6 227 3 190 254 0.0 6 250 4 191 288 0.0 6 286 1 1 192 283 0.0 6 276 7 193 351 0.0 6 347 3 1 194 406 0.0 6 401 5 195 443 0.0 6 433 9 0 0 0 0 1 196 450 0.0 6 448 2 197 545 0.0 6 533 12 198 436 0.0 6 432 4 199 451 0.0 6 443 5 0 1 0 0 2 200 368 0.0 6 360 6 2 201 386 0.0 6 377 8 1 202 451 0.0 6 440 10 1 203 485 0.0 6 479 5 0 0 0 0 1 204 404 0.0 6 394 10 205 427 0.0 6 422 5 206 395 0.0 6 390 4 0 0 0 0 1 207 433 0.0 6 426 5 0 2 208 351 0.0 6 346 5 209 324 0.0 6 319 4 0 1 210 388 0.0 6 377 11 211 673 0.0 6 661 10 0 0 0 1 1 212 620 0.0 6 605 15 213 685 0.0 6 670 15 214 636 0.0 6 621 13 1 1 215 574 0.0 6 568 5 0 0 1 216 582 0.0 6 572 9 1 217 521 0.0 6 512 9 218 458 0.0 6 446 12 219 465 0.0 6 458 6 1 220 750 0.0 6 734 12 2 1 1 221 807 0.0 6 796 11 222 608 0.0 6 596 12 223 537 0.0 6 528 6 2 0 0 1 224 684 0.0 6 669 15 225 490 0.0 6 480 7 1 0 0 1 1 226 423 0.0 6 412 10 1 227 678 0.0 6 666 12 228 635 0.0 6 620 14 1 229 509 0.0 6 500 8 1 230 595 0.0 6 585 9 0 1 231 551 0.0 6 539 11 0 1 232 722 0.0 6 710 12 233 618 0.0 6 602 15 0 0 0 0 1 234 637 0.0 6 623 14 235 895 0.0 6 875 19 1 236 905 0.0 6 882 23 237 786 0.0 6 771 15 238 605 0.0 6 593 11 1 239 776 0.0 6 760 15 1 240 680 0.0 6 668 12 241 523 0.0 6 509 10 3 0 1 242 521 0.0 6 509 12 243 596 0.0 6 586 10 244 497 0.0 6 486 10 1 245 475 0.0 6 461 13 1 246 510 0.0 6 499 10 1 247 446 0.0 6 435 11 248 709 0.0 6 687 22 249 588 0.0 6 565 21 1 0 1 250 433 0.0 6 419 14 251 401 0.0 6 390 10 0 0 0 1 252 447 0.0 6 433 12 2 253 698 0.0 6 684 13 1 254 1010 0.0 6 986 23 1 255 594 0.0 6 575 19 256 606 0.0 6 581 25 257 1018 0.0 6 983 35 258 1143 0.0 6 1102 38 3 259 617 0.0 6 606 11 260 361 0.0 6 349 12 261 368 0.0 6 358 10 262 416 0.0 6 407 9 263 494 0.0 6 482 11 0 1 264 471 0.0 6 450 19 1 1 265 457 0.0 6 425 32 266 1333 0.0 6 1294 39 267 1468 0.0 6 1440 28 268 1113 0.0 6 1078 34 1 269 1226 0.0 6 1192 34 270 1231 0.0 6 1194 36 1 271 1063 0.0 6 1038 25 272 972 0.0 6 944 27 1 273 584 0.0 6 571 13 274 1301 0.0 6 1269 28 1 1 0 2 275 1133 0.0 6 1093 34 4 2 276 3203 0.0 6 3140 63 277 14962 0.0 6 14611 341 8 1 0 1 278 7056 0.0 6 6921 130 3 2 279 98 0.0 6 89 7 1 1 280 4 0.0 6 3 0 1 281 1 0.0 6 1 287 1 0.0 6 0 0 0 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 575 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 64 32 0.0 6 32 72 3 0.0 6 3 73 7 0.0 6 7 74 5 0.0 6 5 75 8 0.0 6 8 76 5 0.0 6 5 77 9 0.0 6 9 78 9 0.0 6 9 79 7 0.0 6 7 80 6 0.0 6 6 81 4 0.0 6 4 82 4 0.0 6 4 83 1 0.0 6 1 84 6 0.0 6 6 85 6 0.0 6 6 86 8 0.0 6 8 87 1 0.0 6 1 88 1 0.0 6 1 89 3 0.0 6 3 91 4 0.0 6 4 92 3 0.0 6 3 93 5 0.0 6 5 94 2 0.0 6 2 95 5 0.0 6 5 96 3 0.0 6 3 97 1 0.0 6 1 98 3 0.0 6 3 99 7 0.0 6 7 100 1 0.0 6 1 101 3 0.0 6 3 102 2 0.0 6 2 103 1 0.0 6 1 104 4 0.0 6 4 105 4 0.0 6 4 106 1 0.0 6 1 107 4 0.0 6 4 108 1 0.0 6 1 109 2 0.0 6 2 110 9 0.0 6 9 111 12 0.0 6 12 112 3 0.0 6 3 113 2 0.0 6 2 114 6 0.0 6 6 116 2 0.0 6 2 117 3 0.0 6 3 118 2 0.0 6 2 119 2 0.0 6 2 120 2 0.0 6 2 121 2 0.0 6 2 122 4 0.0 6 4 123 2 0.0 6 2 124 3 0.0 6 3 125 3 0.0 6 3 126 3 0.0 6 3 127 2 0.0 6 2 128 2 0.0 6 2 129 1 0.0 6 1 130 3 0.0 6 3 131 1 0.0 6 1 132 1 0.0 6 1 134 4 0.0 6 4 135 8 0.0 6 8 136 6 0.0 6 6 137 3 0.0 6 3 138 3 0.0 6 3 139 2 0.0 6 2 140 5 0.0 6 5 141 1 0.0 6 1 142 2 0.0 6 2 143 2 0.0 6 2 144 1 0.0 6 1 145 5 0.0 6 5 146 5 0.0 6 5 147 1 0.0 6 1 149 1 0.0 6 1 150 3 0.0 6 3 151 1 0.0 6 1 152 3 0.0 6 3 153 2 0.0 6 2 154 1 0.0 6 1 155 2 0.0 6 2 156 6 0.0 6 6 157 2 0.0 6 2 158 3 0.0 6 3 159 2 0.0 6 2 160 2 0.0 6 2 161 2 0.0 6 2 162 1 0.0 6 1 163 3 0.0 6 3 164 4 0.0 6 4 165 3 0.0 6 3 166 2 0.0 6 2 167 2 0.0 6 2 168 3 0.0 6 3 169 1 0.0 6 1 170 4 0.0 6 4 172 4 0.0 6 4 173 4 0.0 6 4 174 1 0.0 6 1 175 6 0.0 6 6 176 4 0.0 6 4 177 3 0.0 6 3 178 5 0.0 6 5 179 5 0.0 6 5 180 5 0.0 6 5 181 4 0.0 6 4 182 5 0.0 6 4 0 0 0 0 0 1 183 1 0.0 6 1 185 2 0.0 6 2 186 1 0.0 6 1 187 1 0.0 6 1 188 4 0.0 6 4 189 4 0.0 6 4 190 1 0.0 6 1 191 3 0.0 6 3 192 3 0.0 6 3 193 4 0.0 6 4 194 3 0.0 6 3 195 1 0.0 6 1 196 2 0.0 6 2 197 4 0.0 6 4 198 4 0.0 6 4 199 2 0.0 6 2 201 2 0.0 6 2 202 3 0.0 6 3 203 1 0.0 6 1 204 6 0.0 6 6 205 2 0.0 6 2 206 2 0.0 6 2 207 4 0.0 6 4 208 1 0.0 6 1 209 2 0.0 6 2 210 3 0.0 6 3 211 2 0.0 6 2 212 2 0.0 6 2 213 3 0.0 6 3 215 2 0.0 6 2 216 4 0.0 6 4 217 3 0.0 6 3 219 4 0.0 6 4 221 3 0.0 6 3 222 3 0.0 6 3 223 1 0.0 6 1 224 3 0.0 6 3 225 1 0.0 6 1 226 4 0.0 6 4 227 3 0.0 6 3 228 1 0.0 6 1 229 4 0.0 6 4 230 2 0.0 6 2 231 2 0.0 6 2 232 2 0.0 6 2 233 3 0.0 6 3 234 3 0.0 6 3 235 4 0.0 6 4 236 4 0.0 6 4 237 2 0.0 6 2 238 1 0.0 6 1 240 4 0.0 6 4 241 3 0.0 6 3 242 2 0.0 6 2 243 1 0.0 6 1 244 2 0.0 6 2 246 1 0.0 6 1 247 2 0.0 6 2 248 1 0.0 6 1 251 2 0.0 6 2 254 2 0.0 6 2 255 2 0.0 6 2 256 1 0.0 6 1 257 1 0.0 6 1 260 1 0.0 6 1 261 3 0.0 6 3 262 1 0.0 6 1 264 1 0.0 6 1 266 1 0.0 6 1 267 1 0.0 6 1 269 2 0.0 6 2 270 1 0.0 6 1 273 2 0.0 6 2 279 1 0.0 6 1 280 1 0.0 6 1 285 1 0.0 6 1 288 1 0.0 6 1 289 1 0.0 6 1 295 1 0.0 6 1 298 1 0.0 6 1 300 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 91495 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 5.6 2 1 9 2 0.4 2 1 1 11 6 0.0 3 5 0 0 1 12 5 0.0 3 3 1 1 13 12 0.0 3 10 0 2 14 6 0.0 4 5 1 15 2 0.0 4 1 1 16 13 0.0 4 12 0 1 17 8 0.0 5 4 4 18 10 0.0 5 10 19 15 0.0 5 11 3 1 20 89 0.0 6 84 5 21 59 0.0 6 57 1 1 22 53 0.0 6 50 3 23 36 0.0 6 35 1 24 33 0.0 6 32 1 25 21 0.0 6 19 2 26 24 0.0 6 23 1 27 15 0.0 6 14 1 28 15 0.0 6 14 1 29 20 0.0 6 18 2 30 28 0.0 6 25 3 31 23 0.0 6 22 1 32 21 0.0 6 18 3 33 10 0.0 6 9 1 34 17 0.0 6 14 3 35 18 0.0 6 17 1 36 9 0.0 6 9 37 20 0.0 6 20 38 24 0.0 6 24 39 17 0.0 6 16 1 40 8 0.0 6 8 41 11 0.0 6 7 3 1 42 15 0.0 6 14 1 43 10 0.0 6 10 44 16 0.0 6 16 45 13 0.0 6 12 1 46 18 0.0 6 17 1 47 20 0.0 6 19 1 48 29 0.0 6 27 2 49 15 0.0 6 14 1 50 15 0.0 6 12 3 51 18 0.0 6 16 2 52 20 0.0 6 17 3 53 15 0.0 6 12 3 54 19 0.0 6 16 3 55 34 0.0 6 31 3 56 22 0.0 6 20 2 57 31 0.0 6 27 3 1 58 26 0.0 6 25 1 59 17 0.0 6 16 1 60 21 0.0 6 20 1 61 14 0.0 6 11 3 62 19 0.0 6 18 1 63 11 0.0 6 11 64 22 0.0 6 21 1 65 16 0.0 6 15 0 0 1 66 30 0.0 6 29 1 67 27 0.0 6 24 2 0 0 1 68 45 0.0 6 42 2 0 0 0 1 69 140 0.0 6 127 12 0 0 1 70 56 0.0 6 50 6 71 89 0.0 6 82 7 72 48 0.0 6 44 4 73 42 0.0 6 38 4 74 27 0.0 6 25 2 75 25 0.0 6 23 1 0 1 76 46 0.0 6 45 1 77 47 0.0 6 46 1 78 63 0.0 6 61 2 79 29 0.0 6 28 1 80 28 0.0 6 23 5 81 30 0.0 6 27 3 82 54 0.0 6 51 3 83 54 0.0 6 50 4 84 63 0.0 6 61 2 85 82 0.0 6 77 4 0 0 1 86 42 0.0 6 40 2 87 55 0.0 6 52 3 88 39 0.0 6 38 1 89 54 0.0 6 50 4 90 63 0.0 6 62 1 91 54 0.0 6 49 5 92 59 0.0 6 56 3 93 80 0.0 6 69 10 0 0 0 0 1 94 118 0.0 6 110 5 1 1 1 95 71 0.0 6 64 7 96 73 0.0 6 68 4 0 1 97 106 0.0 6 103 1 1 0 1 98 84 0.0 6 81 1 1 0 1 99 56 0.0 6 51 4 1 100 82 0.0 6 80 2 101 66 0.0 6 65 1 102 118 0.0 6 114 4 103 123 0.0 6 117 5 1 104 113 0.0 6 107 6 105 126 0.0 6 115 11 106 159 0.0 6 155 4 107 146 0.0 6 137 8 1 108 86 0.0 6 83 3 109 106 0.0 6 102 3 1 110 113 0.0 6 106 7 111 96 0.0 6 94 1 1 112 119 0.0 6 113 4 1 1 113 107 0.0 6 103 4 114 122 0.0 6 118 4 115 124 0.0 6 119 3 2 116 130 0.0 6 127 2 0 0 0 1 117 100 0.0 6 95 4 0 0 1 118 135 0.0 6 132 1 1 0 1 119 150 0.0 6 142 8 120 217 0.0 6 211 6 121 156 0.0 6 149 6 0 0 1 122 171 0.0 6 166 5 123 202 0.0 6 195 5 0 1 0 0 1 124 197 0.0 6 191 6 125 179 0.0 6 175 4 126 183 0.0 6 177 6 127 237 0.0 6 226 10 0 0 0 1 128 197 0.0 6 193 2 0 0 1 1 129 202 0.0 6 198 4 130 201 0.0 6 197 2 0 1 0 0 1 131 319 0.0 6 312 5 1 0 1 132 401 0.0 6 391 9 1 133 403 0.0 6 393 8 0 0 1 0 1 134 400 0.0 6 389 11 135 585 0.0 6 575 10 136 401 0.0 6 390 8 1 0 0 1 1 137 472 0.0 6 464 8 138 376 0.0 6 368 8 139 403 0.0 6 394 6 1 1 0 0 1 140 436 0.0 6 427 8 0 0 0 0 1 141 513 0.0 6 503 9 1 142 824 0.0 6 815 8 1 143 762 0.0 6 748 9 2 0 2 0 1 144 859 0.0 6 837 22 145 640 0.0 6 625 13 1 0 1 146 759 0.0 6 742 14 1 0 0 2 147 646 0.0 6 632 11 2 0 1 148 750 0.0 6 741 9 149 848 0.0 6 831 16 1 150 1104 0.0 6 1081 21 1 1 151 869 0.0 6 843 23 1 1 0 1 152 1468 0.0 6 1431 32 2 1 0 1 1 153 930 0.0 6 906 20 1 1 2 154 1194 0.0 6 1163 24 0 2 5 155 1015 0.0 6 999 15 0 0 0 1 156 842 0.0 6 818 19 1 1 1 0 2 157 984 0.0 6 963 21 158 1016 0.0 6 989 24 2 0 1 159 804 0.0 6 789 14 0 0 0 0 1 160 1016 0.0 6 1003 10 1 0 0 1 1 161 751 0.0 6 743 6 1 0 1 162 763 0.0 6 745 17 0 0 1 163 760 0.0 6 738 20 2 164 716 0.0 6 701 9 5 0 1 165 735 0.0 6 715 17 2 1 166 865 0.0 6 847 18 167 505 0.0 6 492 13 168 1120 0.0 6 1101 17 0 1 1 169 492 0.0 6 486 6 170 776 0.0 6 755 19 1 0 1 171 1021 0.0 6 992 28 0 0 1 172 518 0.0 6 510 7 0 1 173 637 0.0 6 625 10 1 1 174 854 0.0 6 831 21 0 0 2 175 1021 0.0 6 999 20 0 0 2 176 803 0.0 6 773 27 1 0 1 0 1 177 644 0.0 6 629 15 178 1267 0.0 6 1236 30 0 0 1 179 894 0.0 6 865 28 0 1 180 1016 0.0 6 980 35 0 1 181 792 0.0 6 767 21 3 1 182 436 0.0 6 421 14 0 0 1 183 741 0.0 6 726 11 3 1 184 192 0.0 6 187 3 0 0 1 1 185 403 0.0 6 390 10 2 0 1 186 1760 0.0 6 1715 42 3 187 1330 0.0 6 1297 32 1 188 1677 0.0 6 1628 46 2 1 189 1648 0.0 6 1608 38 1 0 1 190 1992 0.0 6 1934 54 1 0 1 2 191 1426 0.0 6 1387 35 1 2 1 192 861 0.0 6 818 40 2 0 0 0 1 193 723 0.0 6 696 22 3 1 1 194 1247 0.0 6 1206 39 1 0 1 195 1633 0.0 6 1576 56 1 196 1811 0.0 6 1773 36 0 1 1 197 415 0.0 6 393 19 3 198 1483 0.0 6 1429 51 1 0 2 199 3222 0.0 6 3141 79 0 0 2 200 1773 0.0 6 1727 43 2 1 201 1302 0.0 6 1250 51 1 202 1443 0.0 6 1407 34 2 203 1035 0.0 6 1007 25 3 204 952 0.0 6 918 32 1 0 1 205 326 0.0 6 319 6 0 1 206 408 0.0 6 402 6 207 207 0.0 6 202 5 208 497 0.0 6 482 14 0 0 1 209 438 0.0 6 428 9 1 210 355 0.0 6 347 6 0 0 2 211 405 0.0 6 392 13 212 398 0.0 6 387 10 1 213 216 0.0 6 203 12 1 214 590 0.0 6 573 16 1 215 435 0.0 6 415 20 216 1931 0.0 6 1869 56 2 0 3 1 217 854 0.0 6 827 25 1 0 1 218 1124 0.0 6 1099 25 219 625 0.0 6 609 16 220 765 0.0 6 736 28 0 0 1 221 1438 0.0 6 1396 41 1 222 375 0.0 6 361 13 1 223 1186 0.0 6 1150 33 1 0 2 224 121 0.0 6 115 6 225 216 0.0 6 208 7 0 0 1 226 138 0.0 6 135 3 227 131 0.0 6 127 4 228 56 0.0 6 52 4 229 144 0.0 6 141 3 230 107 0.0 6 101 5 1 231 136 0.0 6 131 5 232 187 0.0 6 184 3 233 287 0.0 6 282 5 234 114 0.0 6 112 2 235 62 0.0 6 62 236 46 0.0 6 44 2 237 54 0.0 6 54 238 11 0.0 6 10 1 239 11 0.0 6 10 0 0 0 1 240 92 0.0 6 88 3 0 0 0 0 1 241 94 0.0 6 90 4 242 64 0.0 6 63 1 243 221 0.0 6 215 6 244 118 0.0 6 116 2 245 64 0.0 6 60 4 246 99 0.0 6 97 2 247 24 0.0 6 24 248 39 0.0 6 38 1 249 62 0.0 6 60 2 250 29 0.0 6 29 251 21 0.0 6 21 252 8 0.0 6 8 253 35 0.0 6 35 254 6 0.0 6 5 1 255 5 0.0 6 5 256 2 0.0 6 2 259 2 0.0 6 2 260 1 0.0 6 1 262 1 0.0 6 1 263 1 0.0 6 1 269 1 0.0 6 1 279 7 0.0 6 7 280 12 0.0 6 12 281 50 0.0 6 49 0 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 396 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 47 1 0.0 6 0 1 52 3 0.0 6 3 69 1 0.0 6 1 70 9 0.0 6 9 71 3 0.0 6 3 72 5 0.0 6 5 73 6 0.0 6 6 74 1 0.0 6 1 75 5 0.0 6 5 76 6 0.0 6 6 77 7 0.0 6 7 78 4 0.0 6 4 79 5 0.0 6 5 80 5 0.0 6 5 81 4 0.0 6 4 82 3 0.0 6 3 83 5 0.0 6 5 84 4 0.0 6 4 85 1 0.0 6 1 86 4 0.0 6 4 87 4 0.0 6 4 88 1 0.0 6 1 90 5 0.0 6 5 91 1 0.0 6 1 92 2 0.0 6 2 93 5 0.0 6 5 94 5 0.0 6 5 95 5 0.0 6 5 97 3 0.0 6 3 98 2 0.0 6 2 99 5 0.0 6 5 100 5 0.0 6 5 101 4 0.0 6 4 102 5 0.0 6 5 103 1 0.0 6 1 104 2 0.0 6 2 105 2 0.0 6 2 107 11 0.0 6 11 108 9 0.0 6 9 109 3 0.0 6 3 110 2 0.0 6 2 112 3 0.0 6 3 113 2 0.0 6 2 114 5 0.0 6 5 115 2 0.0 6 2 116 1 0.0 6 1 117 1 0.0 6 1 118 2 0.0 6 2 119 2 0.0 6 2 120 5 0.0 6 5 121 4 0.0 6 4 122 2 0.0 6 2 123 5 0.0 6 5 124 6 0.0 6 6 125 2 0.0 6 2 126 2 0.0 6 2 127 1 0.0 6 1 128 1 0.0 6 1 129 5 0.0 6 5 130 3 0.0 6 3 131 3 0.0 6 3 132 3 0.0 6 3 133 3 0.0 6 3 134 2 0.0 6 2 135 1 0.0 6 1 136 2 0.0 6 2 137 1 0.0 6 1 138 2 0.0 6 2 139 3 0.0 6 3 140 3 0.0 6 3 141 1 0.0 6 1 142 2 0.0 6 2 143 7 0.0 6 7 145 2 0.0 6 2 146 3 0.0 6 3 147 1 0.0 6 1 148 1 0.0 6 1 149 4 0.0 6 4 150 2 0.0 6 2 151 2 0.0 6 2 152 5 0.0 6 5 153 2 0.0 6 2 154 2 0.0 6 2 155 2 0.0 6 2 156 5 0.0 6 5 157 1 0.0 6 1 158 3 0.0 6 3 159 4 0.0 6 4 160 4 0.0 6 4 161 6 0.0 6 5 1 162 5 0.0 6 5 164 1 0.0 6 1 165 6 0.0 6 6 166 2 0.0 6 2 167 2 0.0 6 2 168 2 0.0 6 2 169 4 0.0 6 4 170 2 0.0 6 2 171 2 0.0 6 2 172 2 0.0 6 2 173 6 0.0 6 6 174 5 0.0 6 5 176 4 0.0 6 4 177 5 0.0 6 5 179 1 0.0 6 1 180 1 0.0 6 1 182 4 0.0 6 4 183 2 0.0 6 2 184 2 0.0 6 2 185 2 0.0 6 2 186 1 0.0 6 1 187 1 0.0 6 1 188 2 0.0 6 2 190 3 0.0 6 3 191 1 0.0 6 1 192 3 0.0 6 3 193 2 0.0 6 2 194 2 0.0 6 2 195 1 0.0 6 1 196 2 0.0 6 2 197 1 0.0 6 1 198 1 0.0 6 1 199 2 0.0 6 2 203 2 0.0 6 1 0 0 0 1 204 2 0.0 6 2 207 2 0.0 6 2 211 3 0.0 6 3 217 1 0.0 6 1 219 1 0.0 6 1 220 1 0.0 6 1 221 1 0.0 6 1 223 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_02_121_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_02_348_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_02_121_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_02_348_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 6.67 s (78 µs/read; 0.77 M reads/minute). === Summary === Total read pairs processed: 85,321 Read 1 with adapter: 85,316 (100.0%) Read 2 with adapter: 85,321 (100.0%) == Read fate breakdown == Pairs that were too short: 8 (0.0%) Pairs discarded as untrimmed: 5 (0.0%) Pairs written (passing filters): 85,308 (100.0%) Total basepairs processed: 41,429,060 bp Read 1: 23,376,210 bp Read 2: 18,052,850 bp Total written (filtered): 5,481,915 bp (13.2%) Read 1: 2,830,010 bp Read 2: 2,651,905 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 84967 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1333.1 0 1 5 1 83.3 1 1 7 4 5.2 2 1 0 3 11 1 0.0 3 1 15 1 0.0 4 0 1 16 2 0.0 4 1 1 17 1 0.0 5 1 19 1 0.0 5 1 20 53 0.0 6 53 21 18 0.0 6 18 22 10 0.0 6 10 23 16 0.0 6 16 24 7 0.0 6 7 25 10 0.0 6 10 26 10 0.0 6 10 27 2 0.0 6 1 1 28 5 0.0 6 3 0 0 1 0 1 29 4 0.0 6 4 30 7 0.0 6 6 1 31 4 0.0 6 3 0 1 32 4 0.0 6 4 33 5 0.0 6 5 34 2 0.0 6 2 35 2 0.0 6 2 36 3 0.0 6 3 37 7 0.0 6 6 1 38 5 0.0 6 5 39 4 0.0 6 4 40 5 0.0 6 5 41 2 0.0 6 2 42 1 0.0 6 1 43 3 0.0 6 2 1 44 8 0.0 6 8 45 9 0.0 6 9 46 3 0.0 6 3 47 4 0.0 6 3 1 48 1 0.0 6 1 49 2 0.0 6 2 51 4 0.0 6 3 0 1 52 7 0.0 6 7 53 4 0.0 6 4 54 1 0.0 6 1 55 6 0.0 6 5 1 56 6 0.0 6 6 57 6 0.0 6 4 1 1 58 5 0.0 6 5 59 3 0.0 6 3 60 3 0.0 6 2 1 61 4 0.0 6 3 1 62 7 0.0 6 5 2 63 5 0.0 6 5 64 7 0.0 6 7 65 6 0.0 6 5 1 66 2 0.0 6 2 67 3 0.0 6 3 68 5 0.0 6 4 1 69 3 0.0 6 3 70 5 0.0 6 5 71 1 0.0 6 1 72 2 0.0 6 1 1 73 5 0.0 6 4 1 74 5 0.0 6 4 1 75 3 0.0 6 3 76 3 0.0 6 3 77 4 0.0 6 3 0 0 1 78 10 0.0 6 10 79 5 0.0 6 5 80 3 0.0 6 3 81 3 0.0 6 3 82 11 0.0 6 11 83 2 0.0 6 2 84 8 0.0 6 7 1 85 6 0.0 6 6 86 11 0.0 6 11 87 7 0.0 6 7 89 7 0.0 6 7 90 2 0.0 6 2 91 6 0.0 6 6 92 6 0.0 6 6 93 4 0.0 6 4 94 5 0.0 6 5 95 4 0.0 6 4 96 3 0.0 6 3 97 9 0.0 6 8 1 98 14 0.0 6 13 1 99 4 0.0 6 3 1 100 10 0.0 6 10 101 17 0.0 6 17 102 15 0.0 6 15 103 13 0.0 6 13 104 9 0.0 6 8 0 0 1 105 7 0.0 6 7 106 6 0.0 6 6 107 8 0.0 6 7 1 108 8 0.0 6 8 109 8 0.0 6 7 0 0 0 0 0 1 110 11 0.0 6 11 111 12 0.0 6 11 1 112 23 0.0 6 22 0 0 1 113 10 0.0 6 9 0 1 114 20 0.0 6 19 1 115 13 0.0 6 13 116 10 0.0 6 9 1 117 16 0.0 6 16 118 7 0.0 6 7 119 17 0.0 6 17 120 17 0.0 6 16 1 121 28 0.0 6 28 122 31 0.0 6 29 2 123 26 0.0 6 25 1 124 22 0.0 6 21 1 125 19 0.0 6 19 126 40 0.0 6 39 1 127 33 0.0 6 33 128 34 0.0 6 34 129 20 0.0 6 17 3 130 19 0.0 6 19 131 20 0.0 6 19 1 132 23 0.0 6 21 2 133 44 0.0 6 42 2 134 42 0.0 6 42 135 38 0.0 6 38 136 30 0.0 6 30 137 32 0.0 6 31 0 1 138 34 0.0 6 32 2 139 35 0.0 6 33 2 140 34 0.0 6 33 1 141 54 0.0 6 53 1 142 140 0.0 6 136 2 1 1 143 222 0.0 6 221 1 144 281 0.0 6 281 145 211 0.0 6 208 3 146 178 0.0 6 175 3 147 286 0.0 6 284 2 148 216 0.0 6 215 1 149 151 0.0 6 150 1 150 164 0.0 6 163 1 151 142 0.0 6 139 3 152 141 0.0 6 138 3 153 134 0.0 6 134 154 115 0.0 6 113 1 1 155 103 0.0 6 101 2 156 106 0.0 6 106 157 120 0.0 6 120 158 108 0.0 6 106 2 159 143 0.0 6 139 4 160 134 0.0 6 134 161 170 0.0 6 167 3 162 138 0.0 6 138 163 155 0.0 6 154 1 164 142 0.0 6 142 165 170 0.0 6 164 6 166 126 0.0 6 125 1 167 142 0.0 6 140 2 168 128 0.0 6 127 1 169 116 0.0 6 116 170 150 0.0 6 146 3 1 171 189 0.0 6 188 1 172 156 0.0 6 153 3 173 139 0.0 6 135 4 174 144 0.0 6 143 1 175 142 0.0 6 139 3 176 121 0.0 6 119 2 177 182 0.0 6 179 3 178 144 0.0 6 138 5 0 0 0 1 179 192 0.0 6 184 7 1 180 352 0.0 6 349 3 181 235 0.0 6 231 4 182 181 0.0 6 177 4 183 163 0.0 6 160 3 184 197 0.0 6 192 5 185 210 0.0 6 207 3 186 207 0.0 6 201 6 187 197 0.0 6 194 2 1 188 178 0.0 6 176 2 189 213 0.0 6 208 5 190 239 0.0 6 234 5 191 308 0.0 6 301 7 192 254 0.0 6 249 5 193 288 0.0 6 283 5 194 315 0.0 6 308 6 1 195 348 0.0 6 343 5 196 377 0.0 6 369 6 2 197 342 0.0 6 339 3 198 359 0.0 6 353 6 199 281 0.0 6 277 4 200 340 0.0 6 334 6 201 321 0.0 6 318 2 1 202 335 0.0 6 330 5 203 350 0.0 6 344 6 204 388 0.0 6 381 7 205 448 0.0 6 438 10 206 462 0.0 6 452 9 1 207 473 0.0 6 459 13 0 1 208 543 0.0 6 528 13 0 0 1 1 209 373 0.0 6 367 6 210 427 0.0 6 417 9 0 1 211 688 0.0 6 673 13 1 0 1 212 526 0.0 6 509 17 213 779 0.0 6 765 14 214 559 0.0 6 545 14 215 429 0.0 6 425 4 216 438 0.0 6 429 9 217 600 0.0 6 587 13 218 352 0.0 6 346 6 219 380 0.0 6 369 11 220 391 0.0 6 378 13 221 732 0.0 6 716 16 222 573 0.0 6 563 10 223 925 0.0 6 905 19 0 0 1 224 688 0.0 6 672 16 225 662 0.0 6 652 9 1 226 696 0.0 6 687 9 227 590 0.0 6 578 11 1 228 612 0.0 6 608 4 229 490 0.0 6 486 4 230 415 0.0 6 410 5 231 372 0.0 6 369 3 232 362 0.0 6 357 5 233 419 0.0 6 410 8 0 1 234 431 0.0 6 424 6 0 1 235 421 0.0 6 407 13 0 0 0 1 236 550 0.0 6 524 26 237 1087 0.0 6 1065 22 238 387 0.0 6 375 12 239 448 0.0 6 442 6 240 525 0.0 6 514 11 241 339 0.0 6 338 1 242 440 0.0 6 429 11 243 567 0.0 6 551 16 244 724 0.0 6 712 12 245 372 0.0 6 357 15 246 344 0.0 6 337 7 247 572 0.0 6 562 10 248 389 0.0 6 370 19 249 1223 0.0 6 1198 25 250 482 0.0 6 473 8 1 251 399 0.0 6 390 9 252 515 0.0 6 506 8 1 253 750 0.0 6 729 19 0 1 0 0 1 254 1219 0.0 6 1189 30 255 881 0.0 6 844 37 256 1257 0.0 6 1228 26 2 1 257 783 0.0 6 756 25 2 258 1798 0.0 6 1754 43 1 259 701 0.0 6 682 19 260 1117 0.0 6 1085 30 2 261 369 0.0 6 356 13 262 491 0.0 6 484 7 263 679 0.0 6 665 13 1 264 995 0.0 6 979 16 265 324 0.0 6 313 9 1 1 266 795 0.0 6 775 19 1 267 1387 0.0 6 1352 35 268 710 0.0 6 696 13 1 269 695 0.0 6 678 17 270 351 0.0 6 347 4 271 713 0.0 6 694 19 272 1371 0.0 6 1336 35 273 1283 0.0 6 1249 34 274 1879 0.0 6 1828 51 275 2654 0.0 6 2582 71 1 276 3337 0.0 6 3248 82 6 1 277 15014 0.0 6 14701 308 5 278 3374 0.0 6 3292 81 1 279 101 0.0 6 96 4 0 1 280 9 0.0 6 8 0 1 281 3 0.0 6 2 0 0 0 0 1 282 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 349 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 1 0.0 6 0 1 64 11 0.0 6 11 72 1 0.0 6 1 73 9 0.0 6 9 74 7 0.0 6 7 75 7 0.0 6 7 76 6 0.0 6 6 77 5 0.0 6 5 78 9 0.0 6 9 79 2 0.0 6 2 80 2 0.0 6 2 82 3 0.0 6 3 83 3 0.0 6 3 85 2 0.0 6 2 86 1 0.0 6 1 87 2 0.0 6 2 88 4 0.0 6 4 89 2 0.0 6 2 90 1 0.0 6 1 91 2 0.0 6 2 92 2 0.0 6 2 94 2 0.0 6 2 95 3 0.0 6 3 96 1 0.0 6 1 97 1 0.0 6 1 98 3 0.0 6 3 99 3 0.0 6 3 100 2 0.0 6 2 101 1 0.0 6 1 102 1 0.0 6 1 104 4 0.0 6 4 105 1 0.0 6 1 107 1 0.0 6 1 110 4 0.0 6 4 111 5 0.0 6 5 112 4 0.0 6 4 114 1 0.0 6 1 115 1 0.0 6 1 116 2 0.0 6 2 117 2 0.0 6 2 118 2 0.0 6 2 120 1 0.0 6 1 122 2 0.0 6 2 124 4 0.0 6 4 125 3 0.0 6 3 126 4 0.0 6 4 127 1 0.0 6 1 128 2 0.0 6 2 129 2 0.0 6 2 130 1 0.0 6 1 131 2 0.0 6 2 132 2 0.0 6 2 133 2 0.0 6 2 134 5 0.0 6 5 135 2 0.0 6 2 136 1 0.0 6 1 138 2 0.0 6 2 139 3 0.0 6 3 140 1 0.0 6 1 141 3 0.0 6 3 142 3 0.0 6 3 143 2 0.0 6 2 144 3 0.0 6 3 146 2 0.0 6 2 147 1 0.0 6 1 148 1 0.0 6 1 150 1 0.0 6 1 151 2 0.0 6 2 152 1 0.0 6 1 153 1 0.0 6 1 155 1 0.0 6 1 156 1 0.0 6 1 157 2 0.0 6 2 158 2 0.0 6 2 159 3 0.0 6 3 160 3 0.0 6 3 161 3 0.0 6 3 162 1 0.0 6 1 163 3 0.0 6 3 164 4 0.0 6 4 165 1 0.0 6 1 167 3 0.0 6 3 168 2 0.0 6 2 169 1 0.0 6 1 170 4 0.0 6 4 171 4 0.0 6 4 172 1 0.0 6 1 173 7 0.0 6 7 174 2 0.0 6 2 175 3 0.0 6 3 176 2 0.0 6 2 177 1 0.0 6 1 178 2 0.0 6 2 179 5 0.0 6 5 180 3 0.0 6 3 181 3 0.0 6 3 182 2 0.0 6 2 183 3 0.0 6 3 184 2 0.0 6 2 185 3 0.0 6 3 186 3 0.0 6 3 188 1 0.0 6 1 189 2 0.0 6 2 190 2 0.0 6 2 191 3 0.0 6 3 192 4 0.0 6 4 194 3 0.0 6 3 195 2 0.0 6 2 197 1 0.0 6 1 198 3 0.0 6 3 199 4 0.0 6 4 200 1 0.0 6 1 201 1 0.0 6 1 202 4 0.0 6 4 206 1 0.0 6 1 207 2 0.0 6 2 208 1 0.0 6 1 211 2 0.0 6 2 212 5 0.0 6 5 213 2 0.0 6 2 214 1 0.0 6 1 216 2 0.0 6 2 218 1 0.0 6 1 219 2 0.0 6 2 220 1 0.0 6 1 221 1 0.0 6 1 222 1 0.0 6 1 224 1 0.0 6 1 225 1 0.0 6 1 227 1 0.0 6 1 228 2 0.0 6 2 229 1 0.0 6 1 230 1 0.0 6 1 231 3 0.0 6 3 233 1 0.0 6 1 234 1 0.0 6 1 235 2 0.0 6 2 236 2 0.0 6 2 237 2 0.0 6 2 238 1 0.0 6 1 239 1 0.0 6 1 243 2 0.0 6 2 246 1 0.0 6 1 247 1 0.0 6 1 259 1 0.0 6 1 261 1 0.0 6 1 264 1 0.0 6 1 265 1 0.0 6 1 270 1 0.0 6 1 283 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 84945 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 20.8 1 1 10 3 0.1 3 3 11 7 0.0 3 5 2 12 16 0.0 3 14 1 0 1 13 17 0.0 3 14 3 14 15 0.0 4 14 0 1 15 2 0.0 4 2 16 4 0.0 4 3 1 17 29 0.0 5 27 2 18 17 0.0 5 15 2 19 14 0.0 5 13 1 20 45 0.0 6 43 1 1 21 43 0.0 6 42 1 22 38 0.0 6 36 1 1 23 29 0.0 6 29 24 31 0.0 6 31 25 27 0.0 6 24 3 26 14 0.0 6 12 2 27 17 0.0 6 14 2 0 1 28 8 0.0 6 8 29 7 0.0 6 6 1 30 15 0.0 6 12 3 31 13 0.0 6 11 2 32 6 0.0 6 6 33 4 0.0 6 3 1 34 18 0.0 6 17 1 35 15 0.0 6 14 1 36 13 0.0 6 12 1 37 6 0.0 6 5 1 38 13 0.0 6 9 2 1 0 0 1 39 12 0.0 6 12 40 8 0.0 6 8 41 13 0.0 6 11 2 42 9 0.0 6 9 43 8 0.0 6 6 2 44 23 0.0 6 21 2 45 7 0.0 6 7 46 31 0.0 6 30 1 47 25 0.0 6 23 2 48 22 0.0 6 19 3 49 34 0.0 6 33 1 50 22 0.0 6 20 1 1 51 15 0.0 6 13 2 52 30 0.0 6 29 0 0 0 0 0 1 53 23 0.0 6 18 4 1 54 17 0.0 6 13 4 55 39 0.0 6 35 4 56 20 0.0 6 20 57 27 0.0 6 25 2 58 20 0.0 6 19 1 59 23 0.0 6 21 2 60 20 0.0 6 18 2 61 13 0.0 6 11 2 62 9 0.0 6 8 0 0 1 63 13 0.0 6 11 1 1 64 17 0.0 6 14 2 0 0 1 65 16 0.0 6 13 2 0 0 1 66 18 0.0 6 17 1 67 45 0.0 6 42 2 1 68 146 0.0 6 139 7 69 42 0.0 6 39 3 70 73 0.0 6 68 4 1 71 36 0.0 6 33 3 72 64 0.0 6 60 3 1 73 22 0.0 6 20 2 74 23 0.0 6 22 1 75 20 0.0 6 18 2 76 29 0.0 6 27 2 77 31 0.0 6 29 1 1 78 41 0.0 6 39 2 79 43 0.0 6 41 2 80 38 0.0 6 34 4 81 29 0.0 6 27 2 82 32 0.0 6 29 2 1 83 77 0.0 6 74 3 84 66 0.0 6 58 7 1 85 51 0.0 6 48 3 86 38 0.0 6 35 3 87 62 0.0 6 59 3 88 43 0.0 6 42 1 89 41 0.0 6 39 2 90 60 0.0 6 56 4 91 63 0.0 6 57 5 0 1 92 36 0.0 6 33 2 1 93 46 0.0 6 41 5 94 122 0.0 6 113 9 95 60 0.0 6 55 4 0 0 0 1 96 70 0.0 6 69 1 97 56 0.0 6 52 2 2 98 51 0.0 6 47 4 99 63 0.0 6 60 2 0 0 0 0 1 100 66 0.0 6 64 1 0 0 1 101 59 0.0 6 53 5 1 102 89 0.0 6 89 103 91 0.0 6 86 5 104 116 0.0 6 111 3 1 0 0 0 1 105 144 0.0 6 135 9 106 55 0.0 6 53 1 1 107 73 0.0 6 69 4 108 62 0.0 6 59 3 109 66 0.0 6 65 1 110 62 0.0 6 59 3 111 67 0.0 6 64 3 112 79 0.0 6 74 4 1 113 73 0.0 6 72 1 114 91 0.0 6 84 7 115 87 0.0 6 86 1 116 96 0.0 6 91 5 117 58 0.0 6 56 2 118 105 0.0 6 104 1 119 122 0.0 6 117 5 120 127 0.0 6 121 5 1 121 87 0.0 6 83 4 122 121 0.0 6 117 3 0 0 0 0 1 123 133 0.0 6 127 5 1 124 149 0.0 6 147 2 125 141 0.0 6 133 7 1 126 129 0.0 6 123 5 1 127 142 0.0 6 136 6 128 101 0.0 6 98 2 0 0 1 129 108 0.0 6 107 1 130 132 0.0 6 129 3 131 196 0.0 6 192 4 132 238 0.0 6 227 11 133 214 0.0 6 204 9 1 134 271 0.0 6 265 6 135 458 0.0 6 437 18 1 2 136 261 0.0 6 248 10 2 1 137 306 0.0 6 301 5 138 192 0.0 6 188 4 139 240 0.0 6 234 5 1 140 419 0.0 6 402 17 141 449 0.0 6 438 11 142 609 0.0 6 595 14 143 608 0.0 6 597 11 144 721 0.0 6 697 22 2 145 524 0.0 6 518 6 146 558 0.0 6 552 5 1 147 481 0.0 6 472 7 1 0 1 148 502 0.0 6 489 13 149 679 0.0 6 666 11 1 1 150 812 0.0 6 786 24 1 0 1 151 752 0.0 6 728 22 0 2 152 813 0.0 6 790 22 1 153 730 0.0 6 716 13 0 1 154 797 0.0 6 778 16 1 1 1 155 601 0.0 6 589 11 1 156 620 0.0 6 610 8 1 0 0 0 1 157 716 0.0 6 706 9 1 158 870 0.0 6 850 20 159 515 0.0 6 501 14 160 686 0.0 6 673 13 161 510 0.0 6 504 6 162 486 0.0 6 476 9 0 0 1 163 632 0.0 6 617 14 1 164 671 0.0 6 651 20 165 646 0.0 6 629 17 166 776 0.0 6 761 13 1 0 0 1 167 580 0.0 6 564 14 0 0 2 168 1071 0.0 6 1040 26 2 3 169 504 0.0 6 489 11 1 0 1 2 170 553 0.0 6 541 10 0 0 2 171 879 0.0 6 848 29 1 0 1 172 475 0.0 6 463 11 0 0 0 0 1 173 575 0.0 6 564 9 1 0 0 1 174 768 0.0 6 756 12 175 1099 0.0 6 1070 28 0 1 176 700 0.0 6 682 18 177 1037 0.0 6 1018 18 1 178 740 0.0 6 715 22 0 2 1 179 1008 0.0 6 984 21 0 1 1 1 180 858 0.0 6 834 23 0 1 181 1231 0.0 6 1203 26 1 0 0 0 1 182 361 0.0 6 350 10 0 0 1 183 930 0.0 6 907 21 2 184 629 0.0 6 614 14 1 185 616 0.0 6 592 24 186 1793 0.0 6 1742 49 2 187 1192 0.0 6 1157 31 2 0 2 188 1639 0.0 6 1586 48 4 0 1 189 1112 0.0 6 1073 37 1 0 1 190 1910 0.0 6 1853 49 3 0 4 1 191 1382 0.0 6 1344 31 3 2 2 192 1091 0.0 6 1063 27 1 193 1193 0.0 6 1159 28 3 1 1 1 194 926 0.0 6 901 23 0 0 2 195 1444 0.0 6 1401 42 0 0 1 196 1851 0.0 6 1810 40 1 197 1042 0.0 6 1012 28 1 1 198 1569 0.0 6 1519 48 0 1 0 1 199 2140 0.0 6 2082 56 1 1 200 1220 0.0 6 1183 36 1 201 1586 0.0 6 1531 51 1 0 3 202 1108 0.0 6 1071 36 0 0 0 1 203 757 0.0 6 731 26 204 665 0.0 6 653 11 0 1 205 723 0.0 6 706 17 206 263 0.0 6 249 14 207 452 0.0 6 432 20 208 936 0.0 6 918 18 209 654 0.0 6 641 13 210 341 0.0 6 331 7 2 0 0 0 1 211 419 0.0 6 407 12 212 668 0.0 6 653 14 0 0 1 213 669 0.0 6 655 13 1 214 428 0.0 6 418 8 2 215 639 0.0 6 620 18 0 1 216 851 0.0 6 832 19 217 467 0.0 6 458 9 218 510 0.0 6 496 12 1 0 1 219 396 0.0 6 387 9 220 361 0.0 6 347 13 1 221 813 0.0 6 792 21 222 385 0.0 6 370 13 1 0 1 223 727 0.0 6 705 21 0 0 1 224 859 0.0 6 832 25 1 0 0 1 225 545 0.0 6 530 14 1 226 450 0.0 6 432 16 1 1 227 1565 0.0 6 1530 31 2 1 1 228 472 0.0 6 453 19 229 978 0.0 6 955 23 230 108 0.0 6 104 3 1 231 191 0.0 6 184 7 232 357 0.0 6 350 7 233 475 0.0 6 457 18 234 268 0.0 6 264 3 0 0 1 235 142 0.0 6 134 8 236 96 0.0 6 92 4 237 110 0.0 6 108 2 238 139 0.0 6 136 2 0 1 239 72 0.0 6 70 2 240 83 0.0 6 80 2 1 241 50 0.0 6 47 2 0 1 242 328 0.0 6 319 9 243 224 0.0 6 219 5 244 155 0.0 6 150 5 245 88 0.0 6 85 2 0 1 246 111 0.0 6 109 2 247 65 0.0 6 60 5 248 119 0.0 6 117 2 249 165 0.0 6 161 4 250 40 0.0 6 39 1 251 101 0.0 6 98 3 252 188 0.0 6 184 4 253 21 0.0 6 21 254 21 0.0 6 20 1 255 9 0.0 6 8 1 256 1 0.0 6 1 263 2 0.0 6 1 1 265 1 0.0 6 1 266 1 0.0 6 1 267 1 0.0 6 1 277 1 0.0 6 1 278 4 0.0 6 4 279 30 0.0 6 28 1 1 280 40 0.0 6 35 4 0 0 0 0 1 281 3 0.0 6 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 376 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 3 79 1333.1 0 79 4 1 333.3 1 0 1 51 1 0.0 6 1 52 2 0.0 6 2 59 1 0.0 6 1 60 1 0.0 6 0 0 0 0 0 0 1 67 1 0.0 6 1 68 1 0.0 6 1 70 1 0.0 6 1 71 2 0.0 6 2 72 6 0.0 6 6 73 8 0.0 6 8 74 3 0.0 6 3 75 5 0.0 6 5 76 3 0.0 6 3 77 4 0.0 6 4 78 1 0.0 6 1 79 5 0.0 6 5 80 1 0.0 6 1 81 1 0.0 6 1 82 3 0.0 6 3 83 2 0.0 6 2 84 4 0.0 6 4 85 1 0.0 6 1 86 2 0.0 6 2 88 1 0.0 6 1 89 2 0.0 6 2 90 4 0.0 6 4 91 5 0.0 6 5 92 4 0.0 6 4 93 3 0.0 6 3 94 1 0.0 6 1 95 1 0.0 6 1 97 1 0.0 6 1 98 2 0.0 6 2 99 4 0.0 6 4 100 4 0.0 6 4 101 2 0.0 6 2 102 2 0.0 6 2 103 1 0.0 6 1 104 4 0.0 6 4 105 4 0.0 6 4 106 3 0.0 6 3 107 1 0.0 6 1 108 4 0.0 6 4 109 8 0.0 6 8 110 1 0.0 6 1 112 1 0.0 6 1 113 2 0.0 6 2 114 2 0.0 6 2 115 2 0.0 6 2 116 3 0.0 6 3 117 1 0.0 6 1 118 3 0.0 6 3 119 1 0.0 6 1 120 1 0.0 6 1 122 4 0.0 6 4 123 1 0.0 6 1 124 3 0.0 6 2 1 125 3 0.0 6 3 127 1 0.0 6 1 128 3 0.0 6 3 129 2 0.0 6 2 131 1 0.0 6 1 132 2 0.0 6 2 133 5 0.0 6 5 134 1 0.0 6 1 135 1 0.0 6 1 136 3 0.0 6 3 137 3 0.0 6 3 138 1 0.0 6 1 139 3 0.0 6 3 140 3 0.0 6 3 141 3 0.0 6 3 142 1 0.0 6 1 143 1 0.0 6 1 144 3 0.0 6 3 145 2 0.0 6 2 146 2 0.0 6 2 147 1 0.0 6 1 148 1 0.0 6 1 151 1 0.0 6 1 152 4 0.0 6 4 153 3 0.0 6 3 154 2 0.0 6 2 155 1 0.0 6 1 156 3 0.0 6 3 157 8 0.0 6 8 158 4 0.0 6 4 159 4 0.0 6 4 161 2 0.0 6 2 162 2 0.0 6 2 163 6 0.0 6 6 164 1 0.0 6 1 165 2 0.0 6 2 166 1 0.0 6 1 167 2 0.0 6 2 168 1 0.0 6 1 169 2 0.0 6 2 171 2 0.0 6 2 173 2 0.0 6 2 175 6 0.0 6 6 176 3 0.0 6 3 177 4 0.0 6 4 178 2 0.0 6 2 179 1 0.0 6 1 180 1 0.0 6 1 181 2 0.0 6 2 183 1 0.0 6 1 184 2 0.0 6 2 185 4 0.0 6 3 0 0 0 0 1 186 4 0.0 6 4 187 3 0.0 6 3 188 2 0.0 6 2 189 1 0.0 6 1 191 2 0.0 6 2 193 1 0.0 6 1 195 1 0.0 6 1 196 2 0.0 6 2 198 1 0.0 6 1 199 1 0.0 6 1 200 1 0.0 6 1 203 2 0.0 6 2 204 1 0.0 6 1 218 1 0.0 6 1 227 2 0.0 6 2 232 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_03_122_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_03_349_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_03_122_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_03_349_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.79 s (45 µs/read; 1.35 M reads/minute). === Summary === Total read pairs processed: 129,751 Read 1 with adapter: 129,740 (100.0%) Read 2 with adapter: 129,675 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 87 (0.1%) Pairs written (passing filters): 129,664 (99.9%) Total basepairs processed: 68,078,000 bp Read 1: 37,855,603 bp Read 2: 30,222,397 bp Total written (filtered): 8,423,614 bp (12.4%) Read 1: 4,276,167 bp Read 2: 4,147,447 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 129740 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 2027.4 0 2 4 2 506.8 1 2 5 2 126.7 1 1 0 1 6 3 31.7 1 0 0 3 7 4 7.9 2 0 0 4 9 1 0.5 2 1 10 2 0.1 3 1 1 14 1 0.0 4 1 15 4 0.0 4 4 16 5 0.0 4 3 2 17 5 0.0 5 3 0 1 1 19 1 0.0 5 1 20 17 0.0 6 17 21 12 0.0 6 12 22 14 0.0 6 14 23 8 0.0 6 7 1 24 14 0.0 6 12 1 1 25 10 0.0 6 9 1 26 3 0.0 6 3 27 2 0.0 6 2 29 2 0.0 6 2 30 2 0.0 6 2 31 3 0.0 6 2 1 32 3 0.0 6 3 33 8 0.0 6 7 0 0 0 0 1 34 6 0.0 6 5 1 35 1 0.0 6 1 36 3 0.0 6 3 37 11 0.0 6 9 1 0 0 1 38 6 0.0 6 6 39 2 0.0 6 1 0 0 0 0 0 1 40 6 0.0 6 6 41 3 0.0 6 1 1 0 0 0 0 1 42 2 0.0 6 2 43 1 0.0 6 1 44 7 0.0 6 7 45 67 0.0 6 65 2 46 19 0.0 6 19 47 5 0.0 6 4 1 48 6 0.0 6 6 49 2 0.0 6 1 0 0 1 50 2 0.0 6 2 51 4 0.0 6 4 52 4 0.0 6 4 54 7 0.0 6 7 55 5 0.0 6 5 56 1 0.0 6 1 57 10 0.0 6 10 58 5 0.0 6 5 59 5 0.0 6 4 1 60 2 0.0 6 2 61 1 0.0 6 1 62 2 0.0 6 2 63 9 0.0 6 8 1 64 1 0.0 6 1 65 11 0.0 6 11 66 3 0.0 6 3 68 4 0.0 6 4 69 6 0.0 6 6 70 1 0.0 6 0 1 71 1 0.0 6 1 73 3 0.0 6 3 74 9 0.0 6 8 1 75 4 0.0 6 2 2 76 4 0.0 6 4 77 2 0.0 6 2 78 2 0.0 6 2 79 6 0.0 6 6 80 1 0.0 6 1 81 2 0.0 6 2 82 6 0.0 6 6 83 3 0.0 6 2 1 85 2 0.0 6 2 87 13 0.0 6 13 88 4 0.0 6 4 89 1 0.0 6 1 90 3 0.0 6 3 91 2 0.0 6 2 92 2 0.0 6 2 94 3 0.0 6 2 0 1 98 4 0.0 6 4 99 6 0.0 6 5 1 101 2 0.0 6 2 102 3 0.0 6 1 2 103 6 0.0 6 5 1 104 4 0.0 6 2 2 105 13 0.0 6 12 1 107 8 0.0 6 6 2 108 10 0.0 6 10 109 2 0.0 6 2 110 1 0.0 6 1 111 10 0.0 6 9 1 112 9 0.0 6 9 113 3 0.0 6 2 1 114 2 0.0 6 2 115 17 0.0 6 16 1 116 5 0.0 6 3 0 1 1 117 4 0.0 6 4 118 1 0.0 6 1 119 10 0.0 6 8 2 120 8 0.0 6 8 121 17 0.0 6 16 1 122 31 0.0 6 30 1 123 12 0.0 6 12 124 11 0.0 6 10 1 125 12 0.0 6 10 2 126 20 0.0 6 19 1 127 27 0.0 6 21 6 128 28 0.0 6 28 129 22 0.0 6 18 4 130 21 0.0 6 21 131 9 0.0 6 8 1 132 25 0.0 6 25 133 61 0.0 6 57 4 134 49 0.0 6 48 1 135 35 0.0 6 32 2 0 1 136 25 0.0 6 25 137 6 0.0 6 6 138 20 0.0 6 17 3 139 26 0.0 6 25 1 140 13 0.0 6 11 2 141 12 0.0 6 12 142 16 0.0 6 15 1 143 15 0.0 6 15 144 55 0.0 6 52 3 145 38 0.0 6 38 146 36 0.0 6 32 4 147 21 0.0 6 20 1 148 35 0.0 6 33 2 149 21 0.0 6 20 1 150 37 0.0 6 36 1 151 20 0.0 6 20 152 30 0.0 6 29 1 153 48 0.0 6 47 1 154 19 0.0 6 19 155 9 0.0 6 9 156 5 0.0 6 5 157 20 0.0 6 20 158 32 0.0 6 32 159 47 0.0 6 46 1 160 20 0.0 6 19 1 161 46 0.0 6 42 4 162 41 0.0 6 41 163 42 0.0 6 40 2 164 10 0.0 6 10 165 29 0.0 6 27 2 166 45 0.0 6 44 1 167 23 0.0 6 23 168 46 0.0 6 43 3 169 29 0.0 6 28 1 170 25 0.0 6 24 1 171 32 0.0 6 31 1 172 29 0.0 6 28 1 173 16 0.0 6 15 1 174 42 0.0 6 42 175 23 0.0 6 23 176 12 0.0 6 12 177 37 0.0 6 35 2 178 39 0.0 6 37 2 179 78 0.0 6 77 1 180 30 0.0 6 27 3 181 70 0.0 6 69 1 182 121 0.0 6 118 2 1 183 51 0.0 6 45 5 1 184 38 0.0 6 37 0 0 1 185 75 0.0 6 74 1 186 103 0.0 6 102 1 187 115 0.0 6 113 2 188 170 0.0 6 164 6 189 109 0.0 6 106 3 190 102 0.0 6 100 2 191 57 0.0 6 55 2 192 33 0.0 6 30 2 1 193 70 0.0 6 67 3 194 123 0.0 6 120 3 195 90 0.0 6 89 1 196 121 0.0 6 116 5 197 111 0.0 6 107 3 0 0 1 198 123 0.0 6 120 3 199 242 0.0 6 241 1 200 159 0.0 6 155 4 201 161 0.0 6 156 4 1 202 221 0.0 6 217 4 203 127 0.0 6 124 3 204 137 0.0 6 131 5 1 205 287 0.0 6 276 11 206 424 0.0 6 410 14 207 1120 0.0 6 1088 32 208 665 0.0 6 655 10 209 532 0.0 6 510 21 1 210 976 0.0 6 952 23 0 0 1 211 835 0.0 6 818 17 212 583 0.0 6 575 8 213 300 0.0 6 291 8 1 214 399 0.0 6 393 6 215 162 0.0 6 149 11 2 216 229 0.0 6 224 5 217 149 0.0 6 148 1 218 360 0.0 6 352 8 219 235 0.0 6 224 11 220 355 0.0 6 342 12 0 1 221 371 0.0 6 363 8 222 638 0.0 6 623 15 223 415 0.0 6 403 12 224 183 0.0 6 178 5 225 262 0.0 6 257 5 226 203 0.0 6 200 3 227 496 0.0 6 491 5 228 406 0.0 6 398 7 1 229 151 0.0 6 144 7 230 377 0.0 6 367 9 0 1 231 697 0.0 6 680 16 0 1 232 265 0.0 6 258 7 233 356 0.0 6 348 7 1 234 429 0.0 6 421 8 235 223 0.0 6 211 11 1 236 854 0.0 6 845 9 237 234 0.0 6 221 12 1 238 746 0.0 6 732 14 239 451 0.0 6 443 7 0 1 240 240 0.0 6 232 8 241 313 0.0 6 308 5 242 298 0.0 6 289 9 243 281 0.0 6 272 9 244 644 0.0 6 628 16 245 845 0.0 6 828 17 246 607 0.0 6 594 12 0 0 1 247 350 0.0 6 343 7 248 1179 0.0 6 1157 19 3 249 799 0.0 6 771 26 2 250 2789 0.0 6 2739 49 0 1 251 1092 0.0 6 1071 20 1 252 515 0.0 6 497 16 2 253 1013 0.0 6 978 32 3 254 1629 0.0 6 1595 32 2 255 2768 0.0 6 2711 56 1 256 2309 0.0 6 2259 49 0 1 257 3775 0.0 6 3710 62 3 258 1570 0.0 6 1523 47 259 4053 0.0 6 3991 60 2 260 1849 0.0 6 1805 43 1 261 3163 0.0 6 3109 52 2 262 1670 0.0 6 1645 22 2 0 1 263 1063 0.0 6 1037 24 2 264 2095 0.0 6 2056 36 3 265 2762 0.0 6 2712 49 1 266 1239 0.0 6 1206 33 267 2462 0.0 6 2411 49 2 268 2311 0.0 6 2257 54 269 1613 0.0 6 1585 28 270 568 0.0 6 557 11 271 1028 0.0 6 1006 21 0 0 1 272 1000 0.0 6 988 11 0 1 273 1549 0.0 6 1508 41 274 5498 0.0 6 5383 107 5 1 1 1 275 3655 0.0 6 3551 98 5 0 1 276 6290 0.0 6 6150 133 4 1 2 277 41401 0.0 6 40682 699 19 0 1 278 853 0.0 6 696 154 2 1 279 254 0.0 6 246 7 0 0 1 280 5 0.0 6 5 281 2 0.0 6 1 0 0 1 282 1 0.0 6 0 1 284 1 0.0 6 1 285 1 0.0 6 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 0 times === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 129675 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 2027.4 0 0 4 4 1 506.8 1 1 6 1 31.7 1 1 10 3 0.1 3 3 11 4 0.0 3 3 0 1 13 12 0.0 3 10 2 14 7 0.0 4 6 0 1 15 1 0.0 4 0 1 16 7 0.0 4 5 2 17 18 0.0 5 14 4 18 19 0.0 5 17 2 19 3 0.0 5 3 20 46 0.0 6 46 21 51 0.0 6 49 1 1 22 40 0.0 6 40 23 27 0.0 6 24 3 24 23 0.0 6 21 2 25 28 0.0 6 26 2 26 13 0.0 6 13 27 22 0.0 6 21 1 28 19 0.0 6 18 1 29 21 0.0 6 20 1 30 23 0.0 6 22 1 31 7 0.0 6 6 1 32 13 0.0 6 12 1 33 13 0.0 6 13 34 10 0.0 6 8 2 35 13 0.0 6 12 1 36 6 0.0 6 6 37 19 0.0 6 17 2 38 10 0.0 6 10 39 11 0.0 6 9 1 0 0 1 40 16 0.0 6 15 1 41 9 0.0 6 9 42 8 0.0 6 8 43 13 0.0 6 12 1 44 19 0.0 6 18 1 45 3 0.0 6 3 46 26 0.0 6 22 2 2 47 23 0.0 6 22 1 48 10 0.0 6 7 3 49 18 0.0 6 16 2 50 8 0.0 6 6 1 1 51 12 0.0 6 12 52 30 0.0 6 27 3 53 13 0.0 6 11 1 1 54 25 0.0 6 23 2 55 30 0.0 6 25 3 2 56 16 0.0 6 16 57 37 0.0 6 35 2 58 23 0.0 6 18 4 0 0 1 59 14 0.0 6 13 1 60 31 0.0 6 29 1 1 61 13 0.0 6 12 0 1 62 14 0.0 6 12 1 1 63 14 0.0 6 13 1 64 27 0.0 6 26 1 65 22 0.0 6 21 1 66 42 0.0 6 35 7 67 236 0.0 6 231 5 68 56 0.0 6 49 5 0 0 1 1 69 97 0.0 6 95 2 70 17 0.0 6 16 1 71 51 0.0 6 46 5 72 48 0.0 6 44 4 73 19 0.0 6 18 1 74 18 0.0 6 16 2 75 26 0.0 6 25 0 0 0 1 76 42 0.0 6 38 4 77 42 0.0 6 38 3 1 78 84 0.0 6 81 2 0 0 1 79 56 0.0 6 52 4 80 39 0.0 6 36 2 0 0 0 1 81 33 0.0 6 30 2 0 0 1 82 69 0.0 6 65 4 83 70 0.0 6 66 4 84 46 0.0 6 42 3 1 85 23 0.0 6 22 1 86 8 0.0 6 7 1 87 23 0.0 6 22 1 88 13 0.0 6 11 2 89 33 0.0 6 29 4 90 34 0.0 6 32 2 91 22 0.0 6 20 1 0 0 1 92 33 0.0 6 30 3 93 60 0.0 6 56 4 94 80 0.0 6 73 6 1 95 35 0.0 6 31 4 96 54 0.0 6 48 5 1 97 213 0.0 6 202 10 0 0 1 98 57 0.0 6 51 4 1 0 1 99 24 0.0 6 23 1 100 58 0.0 6 52 6 101 53 0.0 6 49 4 102 74 0.0 6 69 5 103 76 0.0 6 69 7 104 265 0.0 6 250 13 2 105 173 0.0 6 167 6 106 27 0.0 6 26 1 107 96 0.0 6 88 8 108 58 0.0 6 53 5 109 22 0.0 6 20 2 110 39 0.0 6 38 1 111 18 0.0 6 16 0 1 0 1 112 20 0.0 6 19 1 113 15 0.0 6 14 1 114 36 0.0 6 32 4 115 110 0.0 6 104 6 116 48 0.0 6 48 117 59 0.0 6 56 2 0 0 1 118 33 0.0 6 29 3 1 119 89 0.0 6 83 4 2 120 54 0.0 6 52 1 0 1 121 68 0.0 6 64 4 122 55 0.0 6 51 4 123 156 0.0 6 149 4 1 1 0 1 124 89 0.0 6 86 3 125 105 0.0 6 102 3 126 164 0.0 6 157 7 127 231 0.0 6 218 13 128 67 0.0 6 64 2 1 129 158 0.0 6 149 9 130 150 0.0 6 141 8 1 131 150 0.0 6 147 3 132 64 0.0 6 59 5 133 79 0.0 6 75 3 1 134 126 0.0 6 121 4 0 0 1 135 157 0.0 6 153 4 136 118 0.0 6 116 1 0 0 1 137 56 0.0 6 55 1 138 266 0.0 6 253 11 1 0 1 139 157 0.0 6 143 13 1 140 139 0.0 6 136 2 0 0 1 141 230 0.0 6 224 6 142 182 0.0 6 174 7 1 143 387 0.0 6 373 11 0 1 2 144 255 0.0 6 243 9 2 0 1 145 116 0.0 6 112 4 146 387 0.0 6 374 11 1 0 0 1 147 173 0.0 6 166 6 1 148 329 0.0 6 318 11 149 306 0.0 6 296 10 150 418 0.0 6 398 18 2 151 367 0.0 6 362 5 152 445 0.0 6 432 13 153 475 0.0 6 459 15 1 154 572 0.0 6 556 16 155 1108 0.0 6 1078 26 2 0 2 156 1285 0.0 6 1245 40 157 1104 0.0 6 1079 24 0 0 1 158 614 0.0 6 602 10 1 0 0 1 159 656 0.0 6 642 13 0 0 1 160 223 0.0 6 214 8 1 161 419 0.0 6 408 9 2 162 434 0.0 6 418 12 2 1 1 163 710 0.0 6 679 30 1 164 1618 0.0 6 1579 38 1 165 671 0.0 6 644 22 2 0 1 1 1 166 1014 0.0 6 986 27 1 167 614 0.0 6 603 11 168 467 0.0 6 448 16 2 0 1 169 638 0.0 6 617 19 0 1 1 170 791 0.0 6 766 25 171 1055 0.0 6 1039 15 0 0 1 172 1435 0.0 6 1402 31 0 0 2 173 662 0.0 6 646 13 2 0 1 174 1780 0.0 6 1737 41 0 1 1 175 1170 0.0 6 1150 18 1 0 1 176 525 0.0 6 500 24 0 1 177 576 0.0 6 563 12 1 178 560 0.0 6 542 17 1 179 751 0.0 6 730 20 1 180 251 0.0 6 244 6 1 181 459 0.0 6 449 10 182 893 0.0 6 873 18 1 0 1 183 1695 0.0 6 1661 32 2 184 593 0.0 6 574 16 0 1 2 185 1164 0.0 6 1147 17 186 411 0.0 6 389 20 1 1 187 1232 0.0 6 1198 30 2 0 2 188 805 0.0 6 786 18 0 0 1 189 1145 0.0 6 1121 24 190 362 0.0 6 353 8 1 191 703 0.0 6 678 24 1 192 1248 0.0 6 1214 33 1 193 1160 0.0 6 1126 31 2 0 1 194 1003 0.0 6 976 25 2 195 983 0.0 6 963 19 0 1 196 765 0.0 6 744 19 1 1 197 519 0.0 6 494 23 0 2 198 1118 0.0 6 1099 19 199 1592 0.0 6 1549 40 1 0 2 200 2280 0.0 6 2237 40 1 1 1 201 1321 0.0 6 1288 32 0 0 1 202 1969 0.0 6 1932 36 0 0 1 203 1442 0.0 6 1408 30 3 0 1 204 1350 0.0 6 1317 32 1 205 1320 0.0 6 1286 34 206 2234 0.0 6 2195 38 1 207 229 0.0 6 221 7 1 208 894 0.0 6 875 19 209 1160 0.0 6 1121 37 1 0 1 210 684 0.0 6 669 13 0 0 2 211 149 0.0 6 138 11 212 497 0.0 6 489 8 213 179 0.0 6 174 4 0 1 214 1033 0.0 6 1017 16 215 442 0.0 6 424 18 216 1321 0.0 6 1297 23 0 0 1 217 569 0.0 6 550 19 218 1623 0.0 6 1586 35 2 219 1882 0.0 6 1854 27 0 0 1 220 241 0.0 6 233 7 0 0 1 221 617 0.0 6 597 15 1 2 2 222 1086 0.0 6 1052 29 4 1 223 2664 0.0 6 2593 64 2 0 5 224 7413 0.0 6 7269 135 4 1 4 225 2639 0.0 6 2565 60 10 2 0 1 1 226 3879 0.0 6 3758 119 0 2 227 8735 0.0 6 8569 157 3 0 5 1 228 1889 0.0 6 1833 53 0 2 1 229 6821 0.0 6 6703 109 5 0 4 230 831 0.0 6 815 15 1 231 4613 0.0 6 4529 81 2 0 1 232 2493 0.0 6 2436 55 0 1 1 233 1257 0.0 6 1221 33 2 0 0 1 234 1595 0.0 6 1564 29 1 0 1 235 513 0.0 6 498 15 236 72 0.0 6 69 2 1 237 178 0.0 6 167 8 3 238 771 0.0 6 753 18 239 436 0.0 6 426 10 240 74 0.0 6 71 3 241 674 0.0 6 665 6 3 242 356 0.0 6 344 11 1 243 856 0.0 6 840 16 244 1340 0.0 6 1311 29 245 158 0.0 6 152 5 0 1 246 493 0.0 6 485 8 247 217 0.0 6 213 3 1 248 1296 0.0 6 1265 31 249 500 0.0 6 490 10 250 143 0.0 6 135 5 2 0 1 251 1293 0.0 6 1265 26 1 1 252 215 0.0 6 210 5 253 117 0.0 6 112 5 254 18 0.0 6 18 255 6 0.0 6 6 256 4 0.0 6 4 257 2 0.0 6 2 258 2 0.0 6 2 259 1 0.0 6 1 260 1 0.0 6 1 261 4 0.0 6 4 262 5 0.0 6 5 263 3 0.0 6 3 264 4 0.0 6 4 265 6 0.0 6 5 1 266 2 0.0 6 2 267 1 0.0 6 1 268 1 0.0 6 1 269 2 0.0 6 2 271 1 0.0 6 1 272 1 0.0 6 1 274 1 0.0 6 1 276 2 0.0 6 2 277 7 0.0 6 7 278 96 0.0 6 94 2 279 64 0.0 6 51 13 280 15 0.0 6 15 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 0 times WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_04_123_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_04_350_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_04_123_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_04_350_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.63 s (31437 µs/read; 0.00 M reads/minute). === Summary === Total read pairs processed: 52 Read 1 with adapter: 52 (100.0%) Read 2 with adapter: 52 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 0 (0.0%) Pairs written (passing filters): 52 (100.0%) Total basepairs processed: 26,501 bp Read 1: 15,443 bp Read 2: 11,058 bp Total written (filtered): 3,224 bp (12.2%) Read 1: 1,716 bp Read 2: 1,508 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 52 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 130 1 0.0 6 1 175 1 0.0 6 1 200 1 0.0 6 1 207 1 0.0 6 1 216 1 0.0 6 1 236 1 0.0 6 1 240 1 0.0 6 1 242 1 0.0 6 1 243 1 0.0 6 1 247 1 0.0 6 1 254 1 0.0 6 1 256 1 0.0 6 0 1 263 1 0.0 6 1 269 1 0.0 6 1 271 1 0.0 6 1 274 1 0.0 6 1 277 4 0.0 6 3 1 278 32 0.0 6 32 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 0 times === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 52 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 19 1 0.0 5 0 1 28 1 0.0 6 1 102 1 0.0 6 1 104 1 0.0 6 1 109 1 0.0 6 1 135 1 0.0 6 1 154 1 0.0 6 1 157 1 0.0 6 1 158 2 0.0 6 1 1 172 1 0.0 6 1 173 1 0.0 6 1 175 2 0.0 6 2 177 3 0.0 6 3 178 1 0.0 6 1 180 1 0.0 6 1 183 1 0.0 6 1 185 1 0.0 6 1 188 1 0.0 6 1 189 1 0.0 6 1 191 1 0.0 6 1 192 3 0.0 6 3 194 1 0.0 6 1 195 2 0.0 6 2 196 1 0.0 6 1 199 2 0.0 6 2 200 1 0.0 6 1 201 2 0.0 6 1 1 208 1 0.0 6 1 209 2 0.0 6 2 212 1 0.0 6 1 214 2 0.0 6 1 1 218 2 0.0 6 2 219 1 0.0 6 1 221 1 0.0 6 1 224 2 0.0 6 1 1 227 1 0.0 6 1 240 1 0.0 6 1 244 1 0.0 6 1 249 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 0 times WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_05_124_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_05_351_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_05_124_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_05_351_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 6.10 s (111 µs/read; 0.54 M reads/minute). === Summary === Total read pairs processed: 54,875 Read 1 with adapter: 54,867 (100.0%) Read 2 with adapter: 54,847 (99.9%) == Read fate breakdown == Pairs that were too short: 24 (0.0%) Pairs discarded as untrimmed: 31 (0.1%) Pairs written (passing filters): 54,820 (99.9%) Total basepairs processed: 23,634,747 bp Read 1: 13,376,098 bp Read 2: 10,258,649 bp Total written (filtered): 3,535,168 bp (15.0%) Read 1: 1,862,292 bp Read 2: 1,672,876 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 53566 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 2 214.4 1 1 1 5 1 53.6 1 1 6 1 13.4 1 0 0 1 7 5 3.3 2 1 0 4 9 1 0.2 2 0 1 16 3 0.0 4 3 17 3 0.0 5 3 18 1 0.0 5 1 19 3 0.0 5 2 1 20 90 0.0 6 88 2 21 27 0.0 6 27 22 30 0.0 6 29 1 23 28 0.0 6 27 1 24 16 0.0 6 16 25 17 0.0 6 17 26 17 0.0 6 17 27 16 0.0 6 15 0 0 1 28 8 0.0 6 8 29 10 0.0 6 10 30 4 0.0 6 4 31 11 0.0 6 11 32 5 0.0 6 5 33 11 0.0 6 10 0 0 0 0 0 1 34 4 0.0 6 4 35 5 0.0 6 5 36 2 0.0 6 2 37 3 0.0 6 2 1 38 4 0.0 6 3 0 0 0 0 1 39 8 0.0 6 8 40 7 0.0 6 6 1 41 11 0.0 6 10 0 0 1 42 3 0.0 6 3 43 3 0.0 6 3 44 8 0.0 6 7 1 45 3 0.0 6 2 0 1 46 8 0.0 6 6 1 0 0 0 1 47 5 0.0 6 4 0 1 48 8 0.0 6 7 1 49 5 0.0 6 5 50 6 0.0 6 6 51 2 0.0 6 2 52 2 0.0 6 2 53 4 0.0 6 4 54 2 0.0 6 2 55 4 0.0 6 3 0 0 0 0 0 1 56 7 0.0 6 7 57 6 0.0 6 6 58 7 0.0 6 7 59 7 0.0 6 7 60 6 0.0 6 6 61 2 0.0 6 2 62 4 0.0 6 4 63 8 0.0 6 7 1 64 6 0.0 6 6 65 7 0.0 6 7 66 7 0.0 6 6 1 67 8 0.0 6 8 68 8 0.0 6 8 69 6 0.0 6 6 70 6 0.0 6 5 1 71 15 0.0 6 14 1 72 11 0.0 6 10 1 73 10 0.0 6 10 74 6 0.0 6 6 75 8 0.0 6 7 1 76 5 0.0 6 5 77 8 0.0 6 8 78 8 0.0 6 8 79 1 0.0 6 1 80 9 0.0 6 8 1 81 5 0.0 6 5 82 9 0.0 6 9 83 5 0.0 6 5 84 7 0.0 6 7 85 6 0.0 6 6 86 7 0.0 6 6 0 0 0 0 0 1 87 5 0.0 6 5 88 3 0.0 6 3 89 3 0.0 6 3 90 4 0.0 6 3 1 91 10 0.0 6 9 0 1 92 6 0.0 6 5 1 93 7 0.0 6 7 94 8 0.0 6 8 95 6 0.0 6 6 96 7 0.0 6 7 97 8 0.0 6 8 98 8 0.0 6 8 99 10 0.0 6 10 100 12 0.0 6 10 2 101 7 0.0 6 7 102 15 0.0 6 15 103 12 0.0 6 12 104 23 0.0 6 23 105 13 0.0 6 12 1 106 12 0.0 6 11 1 107 15 0.0 6 15 108 10 0.0 6 10 109 17 0.0 6 17 110 8 0.0 6 7 1 111 13 0.0 6 12 1 112 13 0.0 6 13 113 20 0.0 6 20 114 16 0.0 6 15 1 115 13 0.0 6 13 116 18 0.0 6 18 117 22 0.0 6 22 118 18 0.0 6 18 119 15 0.0 6 15 120 24 0.0 6 24 121 15 0.0 6 15 122 22 0.0 6 21 0 1 123 23 0.0 6 21 2 124 23 0.0 6 21 2 125 24 0.0 6 22 2 126 21 0.0 6 21 127 27 0.0 6 25 2 128 34 0.0 6 34 129 27 0.0 6 26 0 0 0 0 1 130 33 0.0 6 33 131 27 0.0 6 24 2 0 0 0 0 1 132 21 0.0 6 21 133 36 0.0 6 36 134 34 0.0 6 32 2 135 28 0.0 6 28 136 24 0.0 6 24 137 46 0.0 6 45 1 138 54 0.0 6 53 1 139 50 0.0 6 49 1 140 46 0.0 6 46 141 145 0.0 6 144 1 142 220 0.0 6 220 143 327 0.0 6 324 3 144 192 0.0 6 190 2 145 199 0.0 6 198 0 1 146 285 0.0 6 285 147 240 0.0 6 238 2 148 305 0.0 6 304 1 149 270 0.0 6 268 1 1 150 218 0.0 6 218 151 289 0.0 6 287 2 152 215 0.0 6 215 153 188 0.0 6 185 2 0 1 154 248 0.0 6 247 0 0 1 155 179 0.0 6 179 156 268 0.0 6 266 2 157 244 0.0 6 240 4 158 221 0.0 6 221 159 253 0.0 6 251 2 160 177 0.0 6 176 1 161 254 0.0 6 251 2 0 1 162 209 0.0 6 208 1 163 215 0.0 6 214 1 164 269 0.0 6 267 2 165 193 0.0 6 191 2 166 216 0.0 6 215 1 167 228 0.0 6 228 168 174 0.0 6 174 169 249 0.0 6 246 3 170 286 0.0 6 284 1 0 0 0 0 1 171 216 0.0 6 213 2 0 0 0 0 1 172 176 0.0 6 175 1 173 251 0.0 6 251 174 238 0.0 6 238 175 237 0.0 6 235 2 176 178 0.0 6 174 4 177 234 0.0 6 233 1 178 254 0.0 6 251 3 179 576 0.0 6 573 2 1 180 327 0.0 6 327 181 254 0.0 6 252 2 182 290 0.0 6 288 2 183 258 0.0 6 254 4 184 278 0.0 6 274 2 1 0 0 0 1 185 341 0.0 6 338 3 186 301 0.0 6 298 3 187 245 0.0 6 239 6 188 455 0.0 6 453 2 189 301 0.0 6 298 3 190 305 0.0 6 303 2 191 335 0.0 6 330 5 192 369 0.0 6 364 5 193 397 0.0 6 391 6 194 552 0.0 6 548 4 195 571 0.0 6 564 7 196 572 0.0 6 569 1 0 1 0 0 1 197 650 0.0 6 645 5 198 527 0.0 6 520 6 0 1 199 406 0.0 6 400 6 200 476 0.0 6 469 7 201 420 0.0 6 414 5 0 1 202 481 0.0 6 477 3 0 1 203 562 0.0 6 559 2 1 204 539 0.0 6 534 3 1 0 1 205 648 0.0 6 642 5 0 0 0 0 1 206 535 0.0 6 529 6 207 609 0.0 6 604 5 208 485 0.0 6 478 5 2 209 492 0.0 6 486 5 1 210 561 0.0 6 556 4 0 0 1 211 633 0.0 6 629 3 1 212 569 0.0 6 564 5 213 752 0.0 6 745 5 1 1 214 873 0.0 6 865 5 1 1 0 1 215 696 0.0 6 690 5 0 0 0 0 1 216 668 0.0 6 662 5 0 1 217 694 0.0 6 688 6 218 616 0.0 6 615 1 219 534 0.0 6 529 4 0 0 0 0 1 220 531 0.0 6 528 0 2 0 0 1 221 561 0.0 6 559 1 0 0 0 1 222 571 0.0 6 568 2 1 223 621 0.0 6 616 4 0 0 0 0 1 224 636 0.0 6 632 4 225 613 0.0 6 605 8 226 549 0.0 6 546 3 227 511 0.0 6 507 4 228 506 0.0 6 497 7 0 0 0 0 2 229 540 0.0 6 536 3 0 1 230 480 0.0 6 476 3 1 231 522 0.0 6 517 4 1 232 542 0.0 6 536 5 1 233 488 0.0 6 488 234 397 0.0 6 395 2 235 419 0.0 6 417 2 236 436 0.0 6 433 3 237 486 0.0 6 483 1 0 0 1 0 1 238 470 0.0 6 461 8 0 0 0 1 239 530 0.0 6 526 4 240 470 0.0 6 461 8 0 0 0 1 241 382 0.0 6 380 2 242 415 0.0 6 411 4 243 359 0.0 6 355 2 2 244 338 0.0 6 337 1 245 296 0.0 6 295 0 1 246 277 0.0 6 277 247 262 0.0 6 259 2 0 0 0 1 248 298 0.0 6 296 2 249 292 0.0 6 292 250 274 0.0 6 273 1 251 243 0.0 6 242 1 252 250 0.0 6 247 3 253 204 0.0 6 203 1 254 226 0.0 6 226 255 199 0.0 6 198 1 256 265 0.0 6 265 257 266 0.0 6 263 3 258 266 0.0 6 263 1 0 0 0 2 259 225 0.0 6 225 260 167 0.0 6 166 1 261 195 0.0 6 192 3 262 162 0.0 6 159 3 263 158 0.0 6 155 2 1 264 154 0.0 6 152 2 265 152 0.0 6 148 4 266 158 0.0 6 158 267 191 0.0 6 189 2 268 173 0.0 6 171 2 269 161 0.0 6 158 3 270 160 0.0 6 159 1 271 129 0.0 6 127 2 272 201 0.0 6 194 7 273 238 0.0 6 235 3 274 234 0.0 6 232 2 275 259 0.0 6 258 1 276 345 0.0 6 343 2 277 1036 0.0 6 1023 11 2 278 2451 0.0 6 2426 23 2 279 62 0.0 6 61 0 0 1 280 3 0.0 6 3 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1301 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 1 0.0 6 1 63 2 0.0 6 2 64 53 0.0 6 53 65 1 0.0 6 1 72 1 0.0 6 1 73 1 0.0 6 1 74 8 0.0 6 8 75 6 0.0 6 6 76 5 0.0 6 5 77 6 0.0 6 6 78 8 0.0 6 8 79 7 0.0 6 7 80 5 0.0 6 5 81 10 0.0 6 10 82 8 0.0 6 8 83 11 0.0 6 10 0 0 0 0 0 1 84 4 0.0 6 4 85 14 0.0 6 14 86 8 0.0 6 8 87 5 0.0 6 5 88 9 0.0 6 9 89 3 0.0 6 3 90 5 0.0 6 5 91 8 0.0 6 8 92 2 0.0 6 2 93 2 0.0 6 2 94 3 0.0 6 3 95 5 0.0 6 5 96 11 0.0 6 11 97 3 0.0 6 3 98 6 0.0 6 6 99 7 0.0 6 7 100 2 0.0 6 2 101 7 0.0 6 7 102 9 0.0 6 9 103 3 0.0 6 2 1 104 5 0.0 6 5 105 8 0.0 6 8 106 7 0.0 6 7 107 5 0.0 6 5 108 2 0.0 6 2 109 7 0.0 6 7 110 6 0.0 6 6 111 16 0.0 6 16 112 3 0.0 6 3 113 6 0.0 6 6 114 5 0.0 6 5 115 1 0.0 6 1 116 3 0.0 6 3 117 4 0.0 6 4 118 4 0.0 6 4 119 12 0.0 6 12 120 12 0.0 6 12 121 4 0.0 6 4 122 2 0.0 6 2 124 5 0.0 6 5 125 6 0.0 6 6 126 9 0.0 6 9 127 5 0.0 6 5 128 6 0.0 6 6 129 7 0.0 6 7 130 4 0.0 6 4 131 7 0.0 6 7 132 3 0.0 6 3 133 4 0.0 6 4 134 5 0.0 6 5 135 7 0.0 6 7 136 6 0.0 6 6 137 5 0.0 6 5 138 4 0.0 6 4 139 6 0.0 6 6 140 3 0.0 6 3 141 8 0.0 6 8 142 3 0.0 6 3 143 8 0.0 6 8 144 4 0.0 6 4 145 6 0.0 6 6 146 8 0.0 6 8 147 6 0.0 6 6 148 5 0.0 6 5 149 4 0.0 6 4 150 15 0.0 6 15 151 10 0.0 6 10 152 7 0.0 6 7 153 9 0.0 6 9 154 6 0.0 6 6 155 6 0.0 6 6 156 9 0.0 6 8 1 157 6 0.0 6 6 158 6 0.0 6 6 159 9 0.0 6 9 160 4 0.0 6 4 161 11 0.0 6 11 162 6 0.0 6 6 163 10 0.0 6 10 164 7 0.0 6 7 165 10 0.0 6 10 166 8 0.0 6 8 167 6 0.0 6 6 168 7 0.0 6 7 169 7 0.0 6 7 170 7 0.0 6 7 171 7 0.0 6 7 172 8 0.0 6 8 173 4 0.0 6 4 174 6 0.0 6 6 175 8 0.0 6 8 176 9 0.0 6 9 177 10 0.0 6 10 178 7 0.0 6 7 179 9 0.0 6 9 180 8 0.0 6 8 181 8 0.0 6 8 182 10 0.0 6 10 183 6 0.0 6 6 184 4 0.0 6 4 185 2 0.0 6 2 186 8 0.0 6 8 187 8 0.0 6 8 188 10 0.0 6 10 189 10 0.0 6 10 190 16 0.0 6 16 191 11 0.0 6 11 192 15 0.0 6 15 193 10 0.0 6 10 194 11 0.0 6 11 195 5 0.0 6 5 196 7 0.0 6 7 197 6 0.0 6 6 198 8 0.0 6 7 0 0 0 0 0 1 199 10 0.0 6 10 200 6 0.0 6 6 201 4 0.0 6 4 202 11 0.0 6 11 203 4 0.0 6 4 204 20 0.0 6 20 205 9 0.0 6 9 206 11 0.0 6 11 207 6 0.0 6 6 208 5 0.0 6 5 209 4 0.0 6 4 210 9 0.0 6 9 211 6 0.0 6 6 212 8 0.0 6 8 213 10 0.0 6 10 214 3 0.0 6 3 215 7 0.0 6 7 216 8 0.0 6 8 217 7 0.0 6 7 218 3 0.0 6 3 219 8 0.0 6 8 220 9 0.0 6 9 221 11 0.0 6 11 222 8 0.0 6 8 223 2 0.0 6 2 224 4 0.0 6 4 225 6 0.0 6 6 226 3 0.0 6 3 227 4 0.0 6 4 228 9 0.0 6 9 229 7 0.0 6 7 230 7 0.0 6 7 231 6 0.0 6 6 232 7 0.0 6 7 233 9 0.0 6 9 234 5 0.0 6 5 235 2 0.0 6 2 236 3 0.0 6 3 237 5 0.0 6 5 238 5 0.0 6 5 239 2 0.0 6 2 240 7 0.0 6 7 241 4 0.0 6 4 242 5 0.0 6 5 243 3 0.0 6 3 244 1 0.0 6 1 245 2 0.0 6 2 246 4 0.0 6 4 247 4 0.0 6 4 248 1 0.0 6 1 249 6 0.0 6 6 250 6 0.0 6 6 251 4 0.0 6 4 252 3 0.0 6 3 253 8 0.0 6 8 254 2 0.0 6 2 255 3 0.0 6 3 256 1 0.0 6 1 258 4 0.0 6 4 259 3 0.0 6 3 260 6 0.0 6 6 261 1 0.0 6 1 262 1 0.0 6 1 263 1 0.0 6 1 264 3 0.0 6 3 266 4 0.0 6 4 267 1 0.0 6 1 268 2 0.0 6 2 269 1 0.0 6 1 270 2 0.0 6 2 271 2 0.0 6 2 272 3 0.0 6 3 273 3 0.0 6 3 274 2 0.0 6 2 275 2 0.0 6 2 276 1 0.0 6 1 277 1 0.0 6 0 0 0 0 0 0 1 278 1 0.0 6 1 279 2 0.0 6 2 280 2 0.0 6 2 281 2 0.0 6 2 282 1 0.0 6 1 284 1 0.0 6 1 286 1 0.0 6 1 290 1 0.0 6 1 293 1 0.0 6 1 294 2 0.0 6 2 297 1 0.0 6 1 298 1 0.0 6 1 301 1 0.0 6 1 303 1 0.0 6 1 305 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 53886 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 9 1 0.2 2 1 12 3 0.0 3 3 13 11 0.0 3 9 2 14 12 0.0 4 12 16 9 0.0 4 9 17 24 0.0 5 23 1 18 7 0.0 5 7 19 12 0.0 5 10 2 20 100 0.0 6 97 3 21 46 0.0 6 44 2 22 31 0.0 6 29 2 23 29 0.0 6 27 2 24 13 0.0 6 12 1 25 22 0.0 6 22 26 16 0.0 6 14 1 0 0 1 27 12 0.0 6 12 28 12 0.0 6 10 1 0 1 29 10 0.0 6 9 1 30 20 0.0 6 19 0 1 31 15 0.0 6 13 2 32 23 0.0 6 20 2 1 33 8 0.0 6 8 34 7 0.0 6 7 35 9 0.0 6 9 36 16 0.0 6 14 2 37 16 0.0 6 15 1 38 15 0.0 6 14 1 39 10 0.0 6 9 1 40 9 0.0 6 9 41 11 0.0 6 11 42 9 0.0 6 7 1 0 1 43 14 0.0 6 13 1 44 18 0.0 6 16 2 45 11 0.0 6 10 1 46 7 0.0 6 5 2 47 18 0.0 6 16 2 48 14 0.0 6 13 1 49 19 0.0 6 19 50 22 0.0 6 21 1 51 22 0.0 6 21 1 52 17 0.0 6 17 53 35 0.0 6 33 2 54 20 0.0 6 18 2 55 20 0.0 6 19 1 56 21 0.0 6 20 0 0 0 1 57 23 0.0 6 21 1 0 0 0 1 58 23 0.0 6 23 59 21 0.0 6 21 60 26 0.0 6 26 61 21 0.0 6 18 2 1 62 22 0.0 6 21 1 63 16 0.0 6 16 64 27 0.0 6 25 2 65 35 0.0 6 34 1 66 29 0.0 6 29 67 21 0.0 6 19 1 1 68 46 0.0 6 43 3 69 109 0.0 6 108 1 70 53 0.0 6 50 2 0 0 0 1 71 64 0.0 6 63 1 72 40 0.0 6 39 1 73 54 0.0 6 49 5 74 41 0.0 6 39 2 75 33 0.0 6 30 3 76 38 0.0 6 34 2 2 77 37 0.0 6 33 3 1 78 30 0.0 6 28 2 79 31 0.0 6 30 1 80 36 0.0 6 35 0 1 81 42 0.0 6 41 0 0 0 1 82 44 0.0 6 40 3 0 1 83 54 0.0 6 51 3 84 63 0.0 6 62 0 0 1 85 61 0.0 6 57 4 86 30 0.0 6 28 2 87 35 0.0 6 31 3 0 0 1 88 41 0.0 6 41 89 55 0.0 6 53 1 0 1 90 47 0.0 6 46 0 0 0 1 91 58 0.0 6 54 3 0 1 92 63 0.0 6 61 1 0 1 93 55 0.0 6 54 1 94 81 0.0 6 75 5 1 95 79 0.0 6 77 1 0 0 0 1 96 56 0.0 6 54 2 97 77 0.0 6 73 4 98 81 0.0 6 81 99 85 0.0 6 84 1 100 71 0.0 6 71 101 62 0.0 6 61 1 102 75 0.0 6 73 2 103 73 0.0 6 72 1 104 82 0.0 6 81 1 105 94 0.0 6 90 4 106 162 0.0 6 155 6 0 1 107 94 0.0 6 91 2 1 108 89 0.0 6 87 1 1 109 101 0.0 6 98 3 110 106 0.0 6 102 3 0 1 111 102 0.0 6 95 6 1 112 107 0.0 6 104 3 113 116 0.0 6 109 6 0 1 114 148 0.0 6 143 5 115 119 0.0 6 116 1 1 0 1 116 124 0.0 6 116 8 117 150 0.0 6 143 6 0 1 118 147 0.0 6 141 4 1 0 1 119 163 0.0 6 155 7 0 1 120 167 0.0 6 157 9 0 0 0 0 1 121 176 0.0 6 165 9 1 0 0 0 1 122 156 0.0 6 155 1 123 161 0.0 6 156 4 1 124 209 0.0 6 205 3 0 0 0 0 1 125 214 0.0 6 208 5 0 0 0 0 1 126 226 0.0 6 217 8 0 0 0 0 1 127 247 0.0 6 241 5 0 0 1 128 270 0.0 6 264 5 0 0 0 1 129 248 0.0 6 241 5 0 0 0 1 1 130 283 0.0 6 274 6 0 1 1 1 131 333 0.0 6 328 5 132 358 0.0 6 349 8 0 0 0 1 133 403 0.0 6 396 7 134 384 0.0 6 371 11 0 1 0 0 1 135 363 0.0 6 357 2 1 0 1 2 136 406 0.0 6 395 10 0 0 0 1 137 441 0.0 6 430 4 0 1 2 1 3 138 379 0.0 6 375 4 139 434 0.0 6 425 8 0 0 0 0 1 140 428 0.0 6 419 8 1 141 510 0.0 6 499 9 1 1 142 609 0.0 6 604 5 143 667 0.0 6 656 10 1 144 664 0.0 6 655 8 0 1 145 677 0.0 6 667 10 146 800 0.0 6 788 10 2 147 756 0.0 6 743 11 0 1 1 148 856 0.0 6 840 13 2 0 1 149 887 0.0 6 871 14 0 0 0 2 150 974 0.0 6 952 18 1 0 1 1 1 151 1083 0.0 6 1065 16 0 0 1 1 152 1330 0.0 6 1310 19 0 0 1 153 938 0.0 6 912 23 0 2 1 154 1252 0.0 6 1219 24 2 1 1 0 5 155 1133 0.0 6 1121 10 0 1 0 1 156 969 0.0 6 958 8 0 1 0 2 157 949 0.0 6 936 11 0 0 0 1 1 158 906 0.0 6 889 16 0 0 0 1 159 963 0.0 6 952 9 0 0 1 0 1 160 937 0.0 6 915 17 0 1 2 0 2 161 911 0.0 6 893 17 0 0 0 0 1 162 820 0.0 6 809 8 2 1 163 736 0.0 6 724 11 0 0 0 1 164 726 0.0 6 718 6 0 0 0 1 1 165 714 0.0 6 696 15 0 1 2 166 712 0.0 6 708 2 0 0 0 1 1 167 625 0.0 6 615 9 0 1 168 764 0.0 6 750 12 1 1 169 659 0.0 6 652 5 1 1 170 748 0.0 6 742 6 171 708 0.0 6 693 14 0 0 1 172 687 0.0 6 677 8 0 0 0 1 1 173 699 0.0 6 687 9 1 1 1 174 741 0.0 6 734 6 0 0 1 175 668 0.0 6 656 10 1 1 176 630 0.0 6 626 3 0 0 1 177 704 0.0 6 695 4 0 1 2 2 178 674 0.0 6 662 11 0 0 0 0 1 179 580 0.0 6 575 5 180 527 0.0 6 521 5 1 181 424 0.0 6 420 4 182 387 0.0 6 377 8 0 0 1 0 1 183 348 0.0 6 343 5 184 411 0.0 6 401 8 0 1 1 185 521 0.0 6 516 4 0 0 0 1 186 737 0.0 6 730 7 187 620 0.0 6 614 4 1 0 1 188 528 0.0 6 520 7 0 0 0 1 189 390 0.0 6 385 4 1 190 464 0.0 6 461 2 0 0 0 0 1 191 384 0.0 6 381 3 192 347 0.0 6 338 7 1 0 1 193 344 0.0 6 340 3 0 0 0 1 194 293 0.0 6 289 4 195 300 0.0 6 298 1 0 1 196 294 0.0 6 287 7 197 233 0.0 6 230 2 0 0 0 1 198 302 0.0 6 290 11 0 0 1 199 334 0.0 6 329 5 200 236 0.0 6 232 4 201 207 0.0 6 205 2 202 167 0.0 6 167 203 170 0.0 6 168 2 204 170 0.0 6 167 2 1 205 122 0.0 6 120 2 206 135 0.0 6 130 4 0 0 0 1 207 116 0.0 6 112 4 208 110 0.0 6 108 2 209 120 0.0 6 119 1 210 87 0.0 6 87 211 102 0.0 6 102 212 85 0.0 6 85 213 67 0.0 6 66 1 214 93 0.0 6 89 3 1 215 76 0.0 6 74 2 216 138 0.0 6 134 4 217 81 0.0 6 80 0 0 0 0 1 218 84 0.0 6 83 0 0 0 1 219 70 0.0 6 68 1 1 220 68 0.0 6 64 3 1 221 77 0.0 6 77 222 61 0.0 6 61 223 83 0.0 6 82 1 224 32 0.0 6 32 225 20 0.0 6 20 226 22 0.0 6 22 227 22 0.0 6 22 228 19 0.0 6 19 229 19 0.0 6 19 230 11 0.0 6 11 231 16 0.0 6 15 1 232 14 0.0 6 14 233 14 0.0 6 14 234 6 0.0 6 6 235 4 0.0 6 4 236 6 0.0 6 6 237 1 0.0 6 1 238 2 0.0 6 2 239 3 0.0 6 3 240 3 0.0 6 3 241 3 0.0 6 3 242 5 0.0 6 5 243 13 0.0 6 12 1 244 2 0.0 6 2 245 3 0.0 6 1 2 246 5 0.0 6 4 1 247 3 0.0 6 3 249 3 0.0 6 3 250 1 0.0 6 1 251 2 0.0 6 2 252 1 0.0 6 1 253 3 0.0 6 2 1 255 2 0.0 6 2 257 1 0.0 6 1 278 1 0.0 6 1 279 2 0.0 6 2 280 12 0.0 6 12 281 80 0.0 6 79 1 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 961 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 53 2 0.0 6 2 56 2 0.0 6 2 58 1 0.0 6 1 59 1 0.0 6 1 68 1 0.0 6 1 69 13 0.0 6 13 70 5 0.0 6 5 71 9 0.0 6 9 72 9 0.0 6 9 73 8 0.0 6 8 74 5 0.0 6 5 75 8 0.0 6 8 76 10 0.0 6 10 77 6 0.0 6 5 0 0 1 78 10 0.0 6 10 79 8 0.0 6 8 80 14 0.0 6 14 81 7 0.0 6 7 82 5 0.0 6 5 83 8 0.0 6 8 84 7 0.0 6 7 85 14 0.0 6 14 86 9 0.0 6 9 87 6 0.0 6 6 88 9 0.0 6 9 89 4 0.0 6 4 90 6 0.0 6 6 91 6 0.0 6 6 92 3 0.0 6 3 93 8 0.0 6 8 94 9 0.0 6 9 95 7 0.0 6 7 96 1 0.0 6 1 97 3 0.0 6 3 98 22 0.0 6 22 99 7 0.0 6 7 100 5 0.0 6 5 101 4 0.0 6 4 102 4 0.0 6 4 103 7 0.0 6 7 104 8 0.0 6 8 105 4 0.0 6 4 106 6 0.0 6 6 107 5 0.0 6 5 108 19 0.0 6 19 109 6 0.0 6 6 110 6 0.0 6 6 111 5 0.0 6 5 112 2 0.0 6 2 113 3 0.0 6 3 114 4 0.0 6 4 115 3 0.0 6 3 116 6 0.0 6 5 1 117 28 0.0 6 28 118 4 0.0 6 4 119 7 0.0 6 7 120 3 0.0 6 3 121 2 0.0 6 2 122 8 0.0 6 8 123 12 0.0 6 12 124 9 0.0 6 9 125 5 0.0 6 4 1 126 9 0.0 6 9 127 5 0.0 6 5 128 4 0.0 6 4 129 3 0.0 6 3 130 7 0.0 6 7 131 3 0.0 6 3 132 8 0.0 6 8 133 5 0.0 6 5 134 8 0.0 6 8 135 5 0.0 6 5 136 8 0.0 6 8 137 15 0.0 6 15 138 3 0.0 6 3 139 4 0.0 6 4 140 7 0.0 6 7 141 6 0.0 6 6 142 6 0.0 6 6 143 10 0.0 6 10 144 5 0.0 6 5 145 13 0.0 6 13 146 7 0.0 6 7 147 7 0.0 6 7 148 10 0.0 6 10 149 8 0.0 6 8 150 5 0.0 6 5 151 7 0.0 6 7 152 9 0.0 6 9 153 6 0.0 6 5 0 0 1 154 5 0.0 6 5 155 9 0.0 6 9 156 10 0.0 6 9 1 157 7 0.0 6 7 158 7 0.0 6 7 159 4 0.0 6 4 160 5 0.0 6 5 161 4 0.0 6 4 162 12 0.0 6 12 163 8 0.0 6 8 164 4 0.0 6 4 165 14 0.0 6 14 166 8 0.0 6 8 167 6 0.0 6 6 168 13 0.0 6 13 169 10 0.0 6 10 170 4 0.0 6 4 171 11 0.0 6 11 172 27 0.0 6 27 173 6 0.0 6 6 174 5 0.0 6 5 175 10 0.0 6 10 176 4 0.0 6 4 177 5 0.0 6 5 178 10 0.0 6 10 179 3 0.0 6 3 180 2 0.0 6 2 181 2 0.0 6 2 182 3 0.0 6 3 183 4 0.0 6 4 184 4 0.0 6 4 185 10 0.0 6 10 186 8 0.0 6 8 187 16 0.0 6 16 188 4 0.0 6 4 189 6 0.0 6 6 190 2 0.0 6 2 191 2 0.0 6 2 192 4 0.0 6 4 193 4 0.0 6 4 194 4 0.0 6 4 195 1 0.0 6 1 196 3 0.0 6 3 198 1 0.0 6 1 199 6 0.0 6 6 200 3 0.0 6 3 201 8 0.0 6 8 203 3 0.0 6 3 204 3 0.0 6 3 205 1 0.0 6 0 0 0 0 1 207 1 0.0 6 1 208 2 0.0 6 2 209 1 0.0 6 1 210 2 0.0 6 2 211 2 0.0 6 2 212 2 0.0 6 2 213 1 0.0 6 1 214 4 0.0 6 4 215 1 0.0 6 1 217 1 0.0 6 1 218 4 0.0 6 4 220 1 0.0 6 1 226 2 0.0 6 2 232 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_06_125_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_06_352_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_06_125_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_06_352_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 6.81 s (116 µs/read; 0.52 M reads/minute). === Summary === Total read pairs processed: 58,859 Read 1 with adapter: 58,855 (100.0%) Read 2 with adapter: 58,831 (100.0%) == Read fate breakdown == Pairs that were too short: 33 (0.1%) Pairs discarded as untrimmed: 32 (0.1%) Pairs written (passing filters): 58,794 (99.9%) Total basepairs processed: 25,679,888 bp Read 1: 14,624,052 bp Read 2: 11,055,836 bp Total written (filtered): 3,712,037 bp (14.5%) Read 1: 1,986,443 bp Read 2: 1,725,594 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 57566 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 3 229.9 1 2 1 6 1 14.4 1 0 0 1 7 3 3.6 2 1 0 2 8 1 0.9 2 1 9 1 0.2 2 1 13 1 0.0 3 1 16 1 0.0 4 1 18 1 0.0 5 1 19 1 0.0 5 1 20 49 0.0 6 48 1 21 43 0.0 6 40 3 22 33 0.0 6 33 23 19 0.0 6 19 24 17 0.0 6 17 25 16 0.0 6 16 26 19 0.0 6 19 27 8 0.0 6 8 28 8 0.0 6 8 29 7 0.0 6 7 30 6 0.0 6 6 31 8 0.0 6 7 1 32 2 0.0 6 2 33 6 0.0 6 6 34 6 0.0 6 6 35 1 0.0 6 1 36 4 0.0 6 4 37 2 0.0 6 2 38 2 0.0 6 2 39 7 0.0 6 6 1 40 8 0.0 6 7 1 41 11 0.0 6 11 42 5 0.0 6 5 43 5 0.0 6 5 44 6 0.0 6 6 45 1 0.0 6 1 46 1 0.0 6 1 47 3 0.0 6 3 48 5 0.0 6 5 49 6 0.0 6 6 50 11 0.0 6 10 1 51 4 0.0 6 4 52 6 0.0 6 6 53 4 0.0 6 3 1 54 5 0.0 6 5 55 6 0.0 6 6 56 4 0.0 6 4 57 3 0.0 6 3 58 5 0.0 6 5 59 4 0.0 6 4 60 9 0.0 6 8 1 61 5 0.0 6 5 62 7 0.0 6 7 63 8 0.0 6 8 64 2 0.0 6 2 65 5 0.0 6 5 66 16 0.0 6 16 67 7 0.0 6 7 68 3 0.0 6 2 1 69 11 0.0 6 10 1 70 5 0.0 6 4 0 0 1 71 8 0.0 6 7 1 72 12 0.0 6 11 1 73 6 0.0 6 6 74 8 0.0 6 8 75 10 0.0 6 10 76 4 0.0 6 4 77 7 0.0 6 7 78 8 0.0 6 6 2 79 9 0.0 6 7 2 80 9 0.0 6 9 81 8 0.0 6 7 1 82 7 0.0 6 6 0 0 1 83 16 0.0 6 16 84 12 0.0 6 11 1 85 10 0.0 6 10 86 14 0.0 6 12 2 87 11 0.0 6 11 88 8 0.0 6 7 0 0 0 0 1 89 10 0.0 6 9 1 90 5 0.0 6 5 91 8 0.0 6 6 1 0 0 0 0 1 92 7 0.0 6 7 93 5 0.0 6 5 94 7 0.0 6 5 1 1 95 5 0.0 6 5 96 8 0.0 6 7 0 1 97 12 0.0 6 11 1 98 7 0.0 6 7 99 9 0.0 6 7 1 1 100 10 0.0 6 9 1 101 15 0.0 6 15 102 14 0.0 6 13 1 103 10 0.0 6 10 104 17 0.0 6 17 105 13 0.0 6 12 1 106 9 0.0 6 7 2 107 12 0.0 6 12 108 13 0.0 6 12 1 109 13 0.0 6 13 110 15 0.0 6 15 111 13 0.0 6 12 1 112 13 0.0 6 13 113 9 0.0 6 9 114 19 0.0 6 19 115 15 0.0 6 15 116 14 0.0 6 14 117 19 0.0 6 17 1 0 0 0 1 118 24 0.0 6 23 1 119 17 0.0 6 17 120 29 0.0 6 26 1 0 0 1 1 121 15 0.0 6 14 1 122 20 0.0 6 18 1 0 0 0 1 123 18 0.0 6 18 124 19 0.0 6 19 125 22 0.0 6 20 2 126 31 0.0 6 31 127 28 0.0 6 25 1 1 0 0 0 1 128 27 0.0 6 26 1 129 26 0.0 6 25 1 130 23 0.0 6 23 131 24 0.0 6 22 2 132 22 0.0 6 20 2 133 29 0.0 6 29 134 33 0.0 6 32 1 135 30 0.0 6 30 136 24 0.0 6 24 137 30 0.0 6 30 138 35 0.0 6 35 139 52 0.0 6 51 1 140 127 0.0 6 126 1 141 192 0.0 6 191 1 142 230 0.0 6 229 1 143 155 0.0 6 155 144 125 0.0 6 123 1 0 0 1 145 216 0.0 6 214 2 146 190 0.0 6 189 1 147 202 0.0 6 202 148 184 0.0 6 184 149 159 0.0 6 158 1 150 196 0.0 6 193 3 151 257 0.0 6 257 152 152 0.0 6 152 153 124 0.0 6 124 154 99 0.0 6 97 2 155 148 0.0 6 147 1 156 207 0.0 6 206 1 157 202 0.0 6 201 1 158 187 0.0 6 187 159 186 0.0 6 182 4 160 203 0.0 6 202 1 161 225 0.0 6 223 2 162 171 0.0 6 167 4 163 124 0.0 6 122 2 164 134 0.0 6 130 4 165 116 0.0 6 114 2 166 173 0.0 6 172 1 167 132 0.0 6 130 2 168 175 0.0 6 174 0 1 169 205 0.0 6 205 170 158 0.0 6 155 3 171 242 0.0 6 239 1 0 0 1 1 172 236 0.0 6 235 1 173 276 0.0 6 275 1 174 261 0.0 6 259 0 1 0 1 175 185 0.0 6 181 4 176 233 0.0 6 228 4 0 0 0 0 1 177 199 0.0 6 199 178 289 0.0 6 288 1 179 223 0.0 6 217 6 180 164 0.0 6 161 3 181 273 0.0 6 270 2 0 1 182 235 0.0 6 233 1 0 0 0 0 1 183 245 0.0 6 242 3 184 246 0.0 6 243 2 1 185 311 0.0 6 310 1 186 279 0.0 6 273 6 187 307 0.0 6 306 1 188 305 0.0 6 302 3 189 338 0.0 6 335 2 1 190 292 0.0 6 287 4 1 191 254 0.0 6 252 2 192 381 0.0 6 377 4 193 456 0.0 6 452 3 0 0 0 0 1 194 497 0.0 6 493 3 1 195 619 0.0 6 616 3 196 749 0.0 6 744 5 197 718 0.0 6 709 8 1 198 591 0.0 6 581 10 199 490 0.0 6 488 2 200 492 0.0 6 486 5 0 0 0 1 201 430 0.0 6 424 5 0 0 1 202 644 0.0 6 637 5 1 1 203 524 0.0 6 520 3 0 0 1 204 576 0.0 6 569 7 205 742 0.0 6 738 4 206 624 0.0 6 617 7 207 728 0.0 6 721 5 0 1 0 0 1 208 589 0.0 6 585 4 209 599 0.0 6 592 7 210 709 0.0 6 704 2 1 1 0 0 1 211 746 0.0 6 732 13 1 212 643 0.0 6 634 8 0 1 213 777 0.0 6 768 6 2 1 214 922 0.0 6 912 9 0 0 0 0 1 215 777 0.0 6 774 2 1 216 696 0.0 6 690 4 1 1 217 794 0.0 6 789 5 218 713 0.0 6 703 8 0 1 1 219 611 0.0 6 608 3 220 668 0.0 6 662 6 221 659 0.0 6 654 5 222 668 0.0 6 654 13 1 223 632 0.0 6 627 2 2 1 224 823 0.0 6 814 8 1 225 723 0.0 6 713 9 1 226 653 0.0 6 648 5 227 613 0.0 6 605 6 0 1 0 1 228 668 0.0 6 659 8 0 0 0 0 1 229 570 0.0 6 560 9 0 0 0 1 230 657 0.0 6 654 2 1 231 630 0.0 6 625 5 232 602 0.0 6 599 2 0 0 0 0 1 233 475 0.0 6 471 4 234 508 0.0 6 503 3 0 0 1 1 235 547 0.0 6 543 4 236 503 0.0 6 496 6 0 1 237 545 0.0 6 541 4 238 563 0.0 6 560 2 0 0 1 239 518 0.0 6 514 3 0 0 0 1 240 518 0.0 6 516 1 1 241 518 0.0 6 514 3 1 242 463 0.0 6 462 1 243 485 0.0 6 481 4 244 396 0.0 6 393 3 245 330 0.0 6 328 2 246 294 0.0 6 293 0 1 247 321 0.0 6 320 1 248 339 0.0 6 338 1 249 365 0.0 6 361 3 1 250 370 0.0 6 366 4 251 324 0.0 6 323 1 252 324 0.0 6 320 3 0 0 1 253 311 0.0 6 307 4 254 344 0.0 6 342 2 255 310 0.0 6 308 2 256 259 0.0 6 259 257 309 0.0 6 304 5 258 347 0.0 6 342 5 259 298 0.0 6 295 3 260 304 0.0 6 301 3 261 242 0.0 6 241 0 0 0 0 0 1 262 211 0.0 6 209 2 263 248 0.0 6 245 2 0 0 0 0 1 264 184 0.0 6 182 1 0 1 265 226 0.0 6 224 2 266 205 0.0 6 203 2 267 250 0.0 6 245 5 268 249 0.0 6 245 4 269 203 0.0 6 202 0 1 270 165 0.0 6 165 271 161 0.0 6 161 272 292 0.0 6 286 5 1 273 237 0.0 6 232 4 0 1 274 309 0.0 6 301 8 275 364 0.0 6 363 1 276 562 0.0 6 556 5 0 0 0 1 277 1564 0.0 6 1553 9 2 278 1982 0.0 6 1971 6 3 0 0 2 279 41 0.0 6 39 1 1 280 5 0.0 6 5 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1289 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 2 0.0 6 1 1 64 17 0.0 6 17 72 2 0.0 6 2 73 5 0.0 6 5 74 9 0.0 6 9 75 6 0.0 6 6 77 4 0.0 6 4 78 11 0.0 6 11 79 6 0.0 6 6 80 5 0.0 6 5 81 9 0.0 6 8 1 82 3 0.0 6 3 83 7 0.0 6 7 84 6 0.0 6 6 85 5 0.0 6 5 86 3 0.0 6 3 87 7 0.0 6 7 88 9 0.0 6 9 89 7 0.0 6 7 90 4 0.0 6 4 91 7 0.0 6 7 92 4 0.0 6 4 94 5 0.0 6 5 95 3 0.0 6 3 96 2 0.0 6 2 97 1 0.0 6 1 99 3 0.0 6 3 100 4 0.0 6 4 101 4 0.0 6 4 102 3 0.0 6 3 104 1 0.0 6 1 105 6 0.0 6 6 106 7 0.0 6 7 107 4 0.0 6 4 109 1 0.0 6 1 110 6 0.0 6 6 111 8 0.0 6 8 112 6 0.0 6 6 113 2 0.0 6 2 114 4 0.0 6 4 115 4 0.0 6 4 116 3 0.0 6 3 117 8 0.0 6 8 118 2 0.0 6 1 1 119 5 0.0 6 5 120 6 0.0 6 6 121 5 0.0 6 5 122 1 0.0 6 1 124 3 0.0 6 3 125 3 0.0 6 3 126 6 0.0 6 6 127 2 0.0 6 2 128 4 0.0 6 4 129 7 0.0 6 7 130 6 0.0 6 6 131 6 0.0 6 6 132 5 0.0 6 5 133 1 0.0 6 1 134 5 0.0 6 5 135 9 0.0 6 9 136 1 0.0 6 1 137 5 0.0 6 5 138 7 0.0 6 7 139 8 0.0 6 8 140 12 0.0 6 12 141 6 0.0 6 6 142 7 0.0 6 7 143 5 0.0 6 5 144 7 0.0 6 7 145 6 0.0 6 6 146 3 0.0 6 3 147 4 0.0 6 4 148 9 0.0 6 9 149 6 0.0 6 6 150 2 0.0 6 2 151 3 0.0 6 3 152 5 0.0 6 5 153 7 0.0 6 7 154 13 0.0 6 13 155 7 0.0 6 7 156 6 0.0 6 6 157 6 0.0 6 6 158 4 0.0 6 4 159 13 0.0 6 13 160 8 0.0 6 8 161 8 0.0 6 8 162 7 0.0 6 7 163 11 0.0 6 11 164 14 0.0 6 14 165 12 0.0 6 12 166 10 0.0 6 10 167 6 0.0 6 6 168 11 0.0 6 11 169 6 0.0 6 6 170 4 0.0 6 4 171 7 0.0 6 6 1 172 11 0.0 6 11 173 12 0.0 6 12 174 11 0.0 6 11 175 11 0.0 6 11 176 18 0.0 6 18 177 8 0.0 6 8 178 5 0.0 6 5 179 3 0.0 6 3 180 10 0.0 6 10 181 9 0.0 6 9 182 5 0.0 6 5 183 15 0.0 6 15 184 1 0.0 6 1 185 16 0.0 6 16 186 15 0.0 6 15 187 15 0.0 6 15 188 8 0.0 6 8 189 14 0.0 6 14 190 10 0.0 6 10 191 12 0.0 6 12 192 7 0.0 6 7 193 10 0.0 6 10 194 11 0.0 6 11 195 6 0.0 6 6 196 7 0.0 6 7 197 10 0.0 6 10 198 2 0.0 6 2 199 2 0.0 6 2 200 4 0.0 6 4 201 14 0.0 6 14 202 5 0.0 6 5 203 9 0.0 6 9 204 7 0.0 6 7 205 8 0.0 6 8 206 6 0.0 6 6 207 6 0.0 6 6 208 5 0.0 6 5 209 6 0.0 6 6 210 12 0.0 6 12 211 4 0.0 6 4 212 6 0.0 6 6 213 12 0.0 6 12 214 13 0.0 6 13 215 5 0.0 6 5 216 7 0.0 6 7 217 22 0.0 6 22 218 8 0.0 6 8 219 11 0.0 6 11 220 6 0.0 6 6 221 8 0.0 6 8 222 3 0.0 6 3 223 4 0.0 6 4 224 2 0.0 6 2 225 4 0.0 6 4 226 13 0.0 6 13 227 15 0.0 6 15 228 5 0.0 6 5 229 4 0.0 6 4 230 5 0.0 6 5 231 11 0.0 6 11 232 7 0.0 6 7 233 9 0.0 6 9 234 3 0.0 6 3 235 9 0.0 6 9 236 2 0.0 6 2 237 3 0.0 6 3 238 2 0.0 6 2 240 5 0.0 6 5 241 5 0.0 6 5 242 9 0.0 6 9 243 3 0.0 6 3 244 2 0.0 6 2 245 6 0.0 6 6 246 8 0.0 6 8 247 3 0.0 6 3 248 7 0.0 6 7 249 10 0.0 6 10 250 8 0.0 6 7 1 251 6 0.0 6 6 252 10 0.0 6 10 253 1 0.0 6 1 254 6 0.0 6 6 255 5 0.0 6 5 256 8 0.0 6 8 257 5 0.0 6 5 258 5 0.0 6 4 0 0 0 1 259 2 0.0 6 2 260 3 0.0 6 3 261 7 0.0 6 7 262 3 0.0 6 3 263 4 0.0 6 4 264 1 0.0 6 1 265 5 0.0 6 5 267 1 0.0 6 1 268 2 0.0 6 2 270 1 0.0 6 1 271 3 0.0 6 3 272 2 0.0 6 2 273 3 0.0 6 3 275 1 0.0 6 1 276 2 0.0 6 2 278 1 0.0 6 1 279 3 0.0 6 3 280 2 0.0 6 2 283 1 0.0 6 1 284 7 0.0 6 7 285 2 0.0 6 2 286 2 0.0 6 2 288 1 0.0 6 1 291 1 0.0 6 1 292 1 0.0 6 1 293 1 0.0 6 1 294 2 0.0 6 2 295 1 0.0 6 1 300 1 0.0 6 1 302 2 0.0 6 2 303 2 0.0 6 2 308 2 0.0 6 2 309 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 57877 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 229.9 1 0 1 5 1 57.5 1 1 7 2 3.6 2 2 10 1 0.1 3 1 11 1 0.0 3 1 12 2 0.0 3 1 1 13 2 0.0 3 1 1 14 6 0.0 4 5 1 15 2 0.0 4 2 16 8 0.0 4 5 2 0 1 17 22 0.0 5 20 2 18 4 0.0 5 3 1 19 58 0.0 5 53 5 20 68 0.0 6 67 1 21 61 0.0 6 59 2 22 35 0.0 6 32 3 23 19 0.0 6 19 24 20 0.0 6 17 3 25 21 0.0 6 19 2 26 15 0.0 6 13 2 27 20 0.0 6 18 1 0 0 0 0 1 28 17 0.0 6 14 2 1 29 20 0.0 6 18 2 30 17 0.0 6 15 2 31 14 0.0 6 13 1 32 15 0.0 6 12 0 1 0 0 1 1 33 12 0.0 6 9 3 34 17 0.0 6 17 35 10 0.0 6 8 1 0 0 1 36 14 0.0 6 14 37 16 0.0 6 16 38 9 0.0 6 8 1 39 18 0.0 6 17 1 40 15 0.0 6 14 1 41 12 0.0 6 11 0 0 1 42 14 0.0 6 13 1 43 20 0.0 6 17 3 44 17 0.0 6 16 1 45 14 0.0 6 14 46 15 0.0 6 14 0 0 1 47 7 0.0 6 6 1 48 21 0.0 6 21 49 18 0.0 6 17 1 50 15 0.0 6 15 51 22 0.0 6 20 1 1 52 21 0.0 6 18 2 1 53 22 0.0 6 21 1 54 29 0.0 6 26 2 0 1 55 17 0.0 6 17 56 20 0.0 6 18 1 0 0 0 0 1 57 13 0.0 6 12 1 58 23 0.0 6 20 2 1 59 22 0.0 6 18 3 0 0 0 0 1 60 28 0.0 6 22 5 0 1 61 34 0.0 6 30 3 1 62 29 0.0 6 24 4 1 63 36 0.0 6 32 3 0 0 0 0 1 64 22 0.0 6 20 2 65 26 0.0 6 25 0 1 66 23 0.0 6 22 1 67 48 0.0 6 46 2 68 36 0.0 6 34 2 69 57 0.0 6 55 2 70 130 0.0 6 121 6 1 1 0 0 1 71 57 0.0 6 54 3 72 62 0.0 6 60 2 73 32 0.0 6 32 74 34 0.0 6 33 0 1 75 38 0.0 6 36 1 1 76 34 0.0 6 34 77 46 0.0 6 41 4 0 0 0 0 1 78 35 0.0 6 35 79 41 0.0 6 40 1 80 34 0.0 6 30 4 81 37 0.0 6 35 2 82 49 0.0 6 45 3 0 0 1 83 47 0.0 6 44 3 84 56 0.0 6 54 1 1 85 57 0.0 6 51 6 86 54 0.0 6 51 3 87 37 0.0 6 36 1 88 51 0.0 6 46 4 1 89 42 0.0 6 41 1 90 54 0.0 6 52 2 91 51 0.0 6 46 4 1 92 56 0.0 6 53 2 0 0 0 0 1 93 54 0.0 6 51 3 94 55 0.0 6 54 1 95 67 0.0 6 63 4 96 89 0.0 6 83 6 97 61 0.0 6 59 2 98 72 0.0 6 69 3 99 83 0.0 6 82 1 100 91 0.0 6 84 6 0 0 0 0 1 101 64 0.0 6 64 102 77 0.0 6 75 2 103 90 0.0 6 89 1 104 82 0.0 6 77 3 1 1 105 87 0.0 6 85 2 106 104 0.0 6 101 3 107 149 0.0 6 144 3 0 0 0 1 1 108 92 0.0 6 85 3 2 0 1 0 1 109 82 0.0 6 79 2 0 0 0 0 1 110 105 0.0 6 103 2 111 102 0.0 6 100 2 112 108 0.0 6 104 3 1 113 143 0.0 6 134 7 0 1 0 1 114 133 0.0 6 128 4 1 115 146 0.0 6 139 7 116 147 0.0 6 137 7 1 0 1 0 1 117 121 0.0 6 116 5 118 182 0.0 6 174 7 1 119 164 0.0 6 155 9 120 181 0.0 6 172 8 0 1 121 186 0.0 6 179 3 1 2 1 122 194 0.0 6 187 6 0 0 0 0 1 123 179 0.0 6 170 8 0 0 1 124 168 0.0 6 165 2 0 0 0 0 1 125 177 0.0 6 169 6 0 0 0 1 1 126 203 0.0 6 198 5 127 242 0.0 6 238 4 128 302 0.0 6 287 10 1 1 1 0 2 129 267 0.0 6 258 9 130 251 0.0 6 245 5 1 131 313 0.0 6 300 12 1 132 312 0.0 6 299 11 0 0 1 1 133 360 0.0 6 357 2 1 134 402 0.0 6 391 11 135 370 0.0 6 358 12 136 428 0.0 6 409 18 0 0 1 137 444 0.0 6 425 13 2 1 2 0 1 138 417 0.0 6 400 11 3 0 0 2 1 139 396 0.0 6 387 8 0 0 1 140 487 0.0 6 470 13 2 0 0 0 2 141 505 0.0 6 495 9 0 0 1 142 619 0.0 6 605 11 1 0 0 1 1 143 639 0.0 6 622 14 0 1 1 1 144 580 0.0 6 566 13 0 1 145 675 0.0 6 662 10 0 1 0 0 2 146 674 0.0 6 659 10 1 0 1 1 2 147 747 0.0 6 732 13 0 2 148 769 0.0 6 746 16 3 0 2 1 1 149 809 0.0 6 788 18 1 1 0 1 150 824 0.0 6 805 16 2 1 151 959 0.0 6 933 21 2 1 1 0 1 152 1178 0.0 6 1166 9 1 0 1 0 1 153 1297 0.0 6 1269 23 3 1 0 0 1 154 967 0.0 6 945 20 0 1 0 0 1 155 1134 0.0 6 1107 24 0 2 1 156 1234 0.0 6 1209 21 1 1 2 157 1032 0.0 6 1012 18 1 0 0 0 1 158 1087 0.0 6 1065 20 0 1 1 159 865 0.0 6 845 18 0 1 0 0 1 160 1020 0.0 6 1001 15 0 0 2 0 2 161 996 0.0 6 974 19 1 0 0 1 1 162 852 0.0 6 831 16 2 0 0 0 3 163 896 0.0 6 880 14 1 1 164 784 0.0 6 767 14 1 0 1 1 165 734 0.0 6 715 16 1 1 0 0 1 166 799 0.0 6 780 15 1 0 1 1 1 167 744 0.0 6 731 12 1 168 705 0.0 6 688 14 1 1 1 169 908 0.0 6 888 15 3 0 1 0 1 170 698 0.0 6 687 10 0 0 1 171 845 0.0 6 829 12 1 0 0 0 3 172 820 0.0 6 807 12 0 0 0 1 173 763 0.0 6 754 8 1 174 867 0.0 6 851 13 3 175 807 0.0 6 795 8 1 0 2 0 1 176 801 0.0 6 784 14 0 1 1 0 1 177 747 0.0 6 740 7 178 665 0.0 6 648 15 0 0 0 0 2 179 782 0.0 6 771 8 2 0 0 0 1 180 612 0.0 6 599 12 1 181 626 0.0 6 621 4 0 0 0 0 1 182 501 0.0 6 492 8 0 1 183 460 0.0 6 455 4 0 0 1 184 432 0.0 6 422 10 185 456 0.0 6 449 6 0 1 186 673 0.0 6 662 10 1 187 822 0.0 6 810 10 1 0 0 1 188 791 0.0 6 778 11 0 1 0 0 1 189 512 0.0 6 497 12 1 0 1 1 190 528 0.0 6 520 5 1 0 1 0 1 191 498 0.0 6 490 5 0 0 2 0 1 192 439 0.0 6 431 6 1 0 0 1 193 412 0.0 6 409 2 0 0 0 1 194 419 0.0 6 416 3 195 371 0.0 6 362 8 0 1 196 427 0.0 6 419 8 197 287 0.0 6 284 3 198 254 0.0 6 249 5 199 428 0.0 6 424 3 0 0 1 200 251 0.0 6 246 4 0 1 201 259 0.0 6 256 3 202 205 0.0 6 198 6 0 0 0 0 1 203 190 0.0 6 189 1 204 172 0.0 6 172 205 177 0.0 6 171 6 206 161 0.0 6 159 2 207 139 0.0 6 137 1 1 208 161 0.0 6 159 2 209 144 0.0 6 143 1 210 143 0.0 6 143 211 121 0.0 6 120 1 212 106 0.0 6 101 5 213 98 0.0 6 95 2 0 1 214 83 0.0 6 81 2 215 99 0.0 6 99 216 114 0.0 6 111 3 217 143 0.0 6 140 3 218 79 0.0 6 77 1 1 219 85 0.0 6 81 4 220 61 0.0 6 60 1 221 56 0.0 6 53 2 0 0 0 1 222 83 0.0 6 80 2 1 223 56 0.0 6 56 224 63 0.0 6 63 225 46 0.0 6 46 226 22 0.0 6 22 227 36 0.0 6 33 2 0 1 228 29 0.0 6 29 229 16 0.0 6 15 1 230 18 0.0 6 17 1 231 13 0.0 6 12 1 232 14 0.0 6 13 1 233 24 0.0 6 24 234 17 0.0 6 17 235 11 0.0 6 10 1 236 12 0.0 6 12 237 5 0.0 6 5 238 8 0.0 6 8 239 2 0.0 6 2 240 5 0.0 6 5 241 7 0.0 6 7 242 9 0.0 6 8 1 243 7 0.0 6 7 244 9 0.0 6 9 245 4 0.0 6 3 1 246 3 0.0 6 3 247 2 0.0 6 2 248 6 0.0 6 6 249 1 0.0 6 1 250 1 0.0 6 1 251 5 0.0 6 5 252 1 0.0 6 1 253 3 0.0 6 3 254 6 0.0 6 4 2 256 2 0.0 6 2 257 1 0.0 6 1 280 3 0.0 6 3 281 10 0.0 6 10 282 87 0.0 6 86 1 283 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 954 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 1 0.0 6 0 1 50 3 0.0 6 3 67 1 0.0 6 1 68 4 0.0 6 4 69 4 0.0 6 4 70 5 0.0 6 5 71 14 0.0 6 14 72 6 0.0 6 6 73 1 0.0 6 1 74 9 0.0 6 9 75 7 0.0 6 7 76 11 0.0 6 11 77 7 0.0 6 7 78 6 0.0 6 6 79 6 0.0 6 5 1 80 9 0.0 6 9 81 5 0.0 6 5 82 5 0.0 6 5 83 3 0.0 6 3 84 2 0.0 6 2 85 2 0.0 6 2 86 6 0.0 6 6 87 3 0.0 6 3 88 5 0.0 6 5 89 6 0.0 6 6 90 9 0.0 6 9 91 4 0.0 6 4 92 1 0.0 6 1 93 5 0.0 6 5 94 1 0.0 6 1 95 6 0.0 6 6 96 2 0.0 6 2 97 3 0.0 6 3 98 6 0.0 6 6 99 3 0.0 6 3 100 4 0.0 6 4 101 10 0.0 6 10 102 9 0.0 6 9 103 3 0.0 6 3 104 2 0.0 6 1 1 105 2 0.0 6 2 106 5 0.0 6 5 107 14 0.0 6 14 108 4 0.0 6 4 109 2 0.0 6 2 110 9 0.0 6 9 111 1 0.0 6 1 112 11 0.0 6 11 113 4 0.0 6 4 114 4 0.0 6 4 115 3 0.0 6 3 116 5 0.0 6 5 117 5 0.0 6 5 118 1 0.0 6 1 119 3 0.0 6 2 1 120 3 0.0 6 3 121 5 0.0 6 5 122 6 0.0 6 6 123 2 0.0 6 2 124 9 0.0 6 9 125 10 0.0 6 10 126 3 0.0 6 3 127 5 0.0 6 5 128 13 0.0 6 13 129 3 0.0 6 3 130 1 0.0 6 1 131 13 0.0 6 13 132 5 0.0 6 5 133 9 0.0 6 9 134 4 0.0 6 4 135 2 0.0 6 2 136 13 0.0 6 13 137 7 0.0 6 7 138 3 0.0 6 3 139 7 0.0 6 7 140 8 0.0 6 8 141 2 0.0 6 2 142 3 0.0 6 3 143 6 0.0 6 6 144 11 0.0 6 11 145 4 0.0 6 4 146 6 0.0 6 6 147 1 0.0 6 1 148 11 0.0 6 11 149 7 0.0 6 7 150 9 0.0 6 9 151 6 0.0 6 6 152 12 0.0 6 12 153 2 0.0 6 2 154 4 0.0 6 4 155 4 0.0 6 4 156 6 0.0 6 6 157 8 0.0 6 8 158 9 0.0 6 9 159 21 0.0 6 21 160 10 0.0 6 10 161 8 0.0 6 8 162 6 0.0 6 6 163 7 0.0 6 7 164 19 0.0 6 19 165 8 0.0 6 8 166 10 0.0 6 10 167 14 0.0 6 14 168 10 0.0 6 10 169 6 0.0 6 6 170 12 0.0 6 12 171 15 0.0 6 14 1 172 16 0.0 6 16 173 8 0.0 6 8 174 3 0.0 6 3 175 9 0.0 6 9 176 10 0.0 6 10 177 14 0.0 6 14 178 1 0.0 6 1 179 9 0.0 6 9 180 10 0.0 6 10 181 4 0.0 6 4 182 13 0.0 6 13 183 15 0.0 6 15 184 1 0.0 6 1 185 14 0.0 6 14 186 17 0.0 6 17 187 9 0.0 6 9 188 4 0.0 6 4 189 7 0.0 6 6 0 0 0 1 190 6 0.0 6 6 191 11 0.0 6 11 192 4 0.0 6 4 193 9 0.0 6 9 194 6 0.0 6 6 195 1 0.0 6 1 196 5 0.0 6 4 0 0 1 197 6 0.0 6 6 198 2 0.0 6 2 199 6 0.0 6 6 200 9 0.0 6 9 201 4 0.0 6 3 0 0 1 202 4 0.0 6 4 203 3 0.0 6 3 204 2 0.0 6 2 205 5 0.0 6 5 206 2 0.0 6 2 207 4 0.0 6 4 208 2 0.0 6 2 209 6 0.0 6 6 210 3 0.0 6 3 211 2 0.0 6 2 212 5 0.0 6 5 213 5 0.0 6 5 214 1 0.0 6 1 215 1 0.0 6 1 216 3 0.0 6 3 217 2 0.0 6 2 221 1 0.0 6 1 225 1 0.0 6 1 226 2 0.0 6 2 230 1 0.0 6 1 231 1 0.0 6 1 246 1 0.0 6 1 247 1 0.0 6 1 250 2 0.0 6 2 254 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_15_126_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_15_353_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_15_126_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_15_353_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 9.99 s (88 µs/read; 0.68 M reads/minute). === Summary === Total read pairs processed: 113,210 Read 1 with adapter: 113,196 (100.0%) Read 2 with adapter: 113,148 (99.9%) == Read fate breakdown == Pairs that were too short: 58 (0.1%) Pairs discarded as untrimmed: 75 (0.1%) Pairs written (passing filters): 113,077 (99.9%) Total basepairs processed: 48,535,141 bp Read 1: 26,038,269 bp Read 2: 22,496,872 bp Total written (filtered): 7,741,757 bp (16.0%) Read 1: 3,971,611 bp Read 2: 3,770,146 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 108940 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1768.9 0 0 2 4 5 442.2 1 4 1 5 1 110.6 1 1 6 9 27.6 1 3 0 6 7 6 6.9 2 2 0 4 8 3 1.7 2 3 9 3 0.4 2 3 10 2 0.1 3 2 11 1 0.0 3 1 14 1 0.0 4 1 15 1 0.0 4 1 16 5 0.0 4 5 17 1 0.0 5 0 1 18 1 0.0 5 1 19 5 0.0 5 4 1 20 179 0.0 6 179 21 105 0.0 6 98 7 22 125 0.0 6 118 6 1 23 116 0.0 6 112 4 24 66 0.0 6 62 2 1 0 1 25 49 0.0 6 47 2 26 46 0.0 6 44 2 27 30 0.0 6 30 28 21 0.0 6 21 29 16 0.0 6 15 0 0 0 1 30 18 0.0 6 18 31 15 0.0 6 13 2 32 8 0.0 6 7 1 33 6 0.0 6 6 34 24 0.0 6 23 1 35 9 0.0 6 9 36 8 0.0 6 8 37 11 0.0 6 11 38 5 0.0 6 5 39 14 0.0 6 14 40 3 0.0 6 3 41 7 0.0 6 7 42 8 0.0 6 8 43 11 0.0 6 11 44 7 0.0 6 7 45 11 0.0 6 10 1 46 4 0.0 6 4 47 10 0.0 6 9 1 48 11 0.0 6 10 1 49 9 0.0 6 9 50 7 0.0 6 6 1 51 10 0.0 6 9 1 52 14 0.0 6 14 53 6 0.0 6 6 54 4 0.0 6 3 1 55 6 0.0 6 5 1 56 7 0.0 6 7 57 9 0.0 6 8 1 58 10 0.0 6 9 1 59 2 0.0 6 2 60 15 0.0 6 15 61 18 0.0 6 15 2 1 62 6 0.0 6 4 1 0 0 1 63 3 0.0 6 3 64 14 0.0 6 12 2 65 7 0.0 6 6 1 66 2 0.0 6 2 67 4 0.0 6 4 68 2 0.0 6 2 69 8 0.0 6 8 70 6 0.0 6 5 0 0 0 0 0 1 71 12 0.0 6 11 1 72 18 0.0 6 18 73 10 0.0 6 9 1 74 17 0.0 6 17 75 13 0.0 6 13 76 12 0.0 6 12 77 10 0.0 6 9 1 78 11 0.0 6 11 79 13 0.0 6 13 80 5 0.0 6 5 81 7 0.0 6 5 2 82 10 0.0 6 9 1 83 39 0.0 6 36 3 84 13 0.0 6 13 85 13 0.0 6 12 1 86 13 0.0 6 13 87 8 0.0 6 5 2 1 88 14 0.0 6 14 89 13 0.0 6 13 90 12 0.0 6 11 0 1 91 8 0.0 6 6 2 92 4 0.0 6 4 93 5 0.0 6 5 94 12 0.0 6 12 95 6 0.0 6 6 96 9 0.0 6 9 97 10 0.0 6 10 98 14 0.0 6 14 99 14 0.0 6 13 1 100 18 0.0 6 16 2 101 18 0.0 6 17 1 102 24 0.0 6 24 103 28 0.0 6 27 1 104 38 0.0 6 36 2 105 19 0.0 6 18 1 106 20 0.0 6 20 107 21 0.0 6 20 1 108 17 0.0 6 17 109 36 0.0 6 33 2 0 1 110 33 0.0 6 32 1 111 31 0.0 6 31 112 51 0.0 6 49 2 113 25 0.0 6 25 114 33 0.0 6 33 115 22 0.0 6 22 116 23 0.0 6 23 117 36 0.0 6 35 1 118 40 0.0 6 39 1 119 21 0.0 6 19 2 120 24 0.0 6 24 121 40 0.0 6 39 1 122 26 0.0 6 24 2 123 30 0.0 6 29 1 124 30 0.0 6 30 125 31 0.0 6 31 126 42 0.0 6 42 127 72 0.0 6 72 128 45 0.0 6 44 1 129 35 0.0 6 35 130 56 0.0 6 54 1 1 131 46 0.0 6 46 132 45 0.0 6 44 0 0 0 1 133 93 0.0 6 93 134 53 0.0 6 53 135 68 0.0 6 68 136 57 0.0 6 57 137 50 0.0 6 48 2 138 65 0.0 6 65 139 91 0.0 6 91 140 89 0.0 6 88 1 141 116 0.0 6 116 142 133 0.0 6 132 0 1 143 145 0.0 6 144 1 144 208 0.0 6 206 2 145 1309 0.0 6 1305 4 146 1744 0.0 6 1740 3 0 1 147 373 0.0 6 371 2 148 1073 0.0 6 1072 1 149 234 0.0 6 234 150 117 0.0 6 115 1 0 1 151 103 0.0 6 103 152 109 0.0 6 109 153 148 0.0 6 147 1 154 973 0.0 6 971 2 155 255 0.0 6 253 1 0 1 156 134 0.0 6 133 1 157 170 0.0 6 169 0 1 158 194 0.0 6 193 1 159 311 0.0 6 310 1 160 249 0.0 6 248 1 161 1611 0.0 6 1608 3 162 388 0.0 6 388 163 197 0.0 6 197 164 290 0.0 6 287 3 165 405 0.0 6 404 1 166 2484 0.0 6 2483 0 0 0 0 1 167 1708 0.0 6 1703 5 168 581 0.0 6 579 1 0 0 1 169 2280 0.0 6 2279 1 170 564 0.0 6 563 0 0 0 0 0 1 171 360 0.0 6 360 172 446 0.0 6 443 2 0 0 0 1 173 690 0.0 6 688 1 0 0 1 174 3544 0.0 6 3536 4 0 1 1 2 175 3389 0.0 6 3384 5 176 3341 0.0 6 3334 6 0 1 177 939 0.0 6 938 1 178 2835 0.0 6 2830 5 179 590 0.0 6 590 180 498 0.0 6 495 3 181 384 0.0 6 384 182 2455 0.0 6 2451 4 183 1705 0.0 6 1700 5 184 487 0.0 6 487 185 341 0.0 6 341 186 370 0.0 6 368 2 187 689 0.0 6 686 3 188 3660 0.0 6 3649 10 0 1 189 631 0.0 6 629 2 190 303 0.0 6 300 3 191 561 0.0 6 556 4 1 192 3299 0.0 6 3293 2 2 2 193 651 0.0 6 649 2 194 278 0.0 6 277 1 195 298 0.0 6 297 1 196 422 0.0 6 420 1 0 0 1 197 643 0.0 6 641 2 198 400 0.0 6 400 199 334 0.0 6 332 2 200 364 0.0 6 362 2 201 477 0.0 6 473 4 202 464 0.0 6 461 3 203 721 0.0 6 720 0 0 1 204 1367 0.0 6 1363 2 1 1 205 1022 0.0 6 1016 5 0 1 206 1160 0.0 6 1154 6 207 2198 0.0 6 2191 7 208 3754 0.0 6 3747 7 209 724 0.0 6 721 3 210 932 0.0 6 928 3 0 1 211 1314 0.0 6 1311 3 212 1801 0.0 6 1799 2 213 5239 0.0 6 5230 8 0 1 214 1796 0.0 6 1792 4 215 685 0.0 6 683 2 216 994 0.0 6 987 7 217 1290 0.0 6 1288 2 218 1142 0.0 6 1138 4 219 1466 0.0 6 1460 6 220 3183 0.0 6 3180 2 1 221 241 0.0 6 239 2 222 178 0.0 6 175 3 223 123 0.0 6 121 2 224 248 0.0 6 247 1 225 274 0.0 6 274 226 271 0.0 6 269 2 227 403 0.0 6 400 3 228 254 0.0 6 253 1 229 226 0.0 6 222 3 0 0 1 230 261 0.0 6 260 1 231 337 0.0 6 336 1 232 569 0.0 6 568 0 0 1 233 338 0.0 6 336 2 234 400 0.0 6 398 1 0 1 235 435 0.0 6 433 2 236 469 0.0 6 466 3 237 981 0.0 6 980 1 238 301 0.0 6 301 239 258 0.0 6 256 2 240 406 0.0 6 401 5 241 467 0.0 6 467 242 510 0.0 6 507 3 243 1056 0.0 6 1055 1 244 346 0.0 6 345 1 245 227 0.0 6 223 4 246 195 0.0 6 195 247 308 0.0 6 307 1 248 243 0.0 6 241 2 249 254 0.0 6 253 0 1 250 282 0.0 6 282 251 338 0.0 6 336 2 252 428 0.0 6 426 1 0 1 253 796 0.0 6 793 3 254 225 0.0 6 224 1 255 201 0.0 6 201 256 254 0.0 6 252 2 257 171 0.0 6 165 4 1 0 0 0 1 258 200 0.0 6 198 2 259 198 0.0 6 195 3 260 245 0.0 6 245 261 210 0.0 6 208 1 1 262 466 0.0 6 465 1 263 136 0.0 6 132 4 264 144 0.0 6 142 2 265 151 0.0 6 147 3 0 1 266 120 0.0 6 119 1 267 111 0.0 6 106 5 268 129 0.0 6 128 0 0 1 269 138 0.0 6 135 3 270 116 0.0 6 116 271 135 0.0 6 133 2 272 247 0.0 6 245 2 273 194 0.0 6 192 2 274 443 0.0 6 441 2 275 394 0.0 6 389 5 276 878 0.0 6 870 7 0 1 277 984 0.0 6 978 6 278 1556 0.0 6 1548 7 1 279 71 0.0 6 71 280 11 0.0 6 11 281 1 0.0 6 1 282 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 4256 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 53 2 0.0 6 2 56 1 0.0 6 1 63 1 0.0 6 0 1 71 2 0.0 6 2 73 1 0.0 6 1 74 19 0.0 6 19 75 62 0.0 6 62 76 77 0.0 6 77 77 23 0.0 6 23 78 52 0.0 6 51 1 79 1 0.0 6 1 80 1 0.0 6 1 81 1 0.0 6 0 0 0 0 0 0 1 82 3 0.0 6 3 83 9 0.0 6 9 84 45 0.0 6 45 85 4 0.0 6 4 86 1 0.0 6 1 88 1 0.0 6 1 89 1 0.0 6 1 90 18 0.0 6 18 91 63 0.0 6 63 92 5 0.0 6 5 93 1 0.0 6 1 94 1 0.0 6 1 95 38 0.0 6 38 96 108 0.0 6 108 97 68 0.0 6 68 98 32 0.0 6 32 99 93 0.0 6 93 100 2 0.0 6 2 101 1 0.0 6 1 102 6 0.0 6 6 103 35 0.0 6 35 104 158 0.0 6 156 2 105 144 0.0 6 143 1 106 124 0.0 6 124 107 60 0.0 6 60 108 111 0.0 6 111 109 8 0.0 6 8 110 5 0.0 6 5 111 36 0.0 6 36 112 100 0.0 6 100 113 56 0.0 6 56 114 2 0.0 6 2 115 4 0.0 6 4 116 5 0.0 6 5 117 65 0.0 6 65 118 158 0.0 6 158 119 15 0.0 6 15 120 4 0.0 6 4 121 53 0.0 6 53 122 120 0.0 6 119 1 123 9 0.0 6 8 1 129 1 0.0 6 1 130 1 0.0 6 1 135 1 0.0 6 1 136 3 0.0 6 3 137 70 0.0 6 70 138 207 0.0 6 207 139 11 0.0 6 10 1 140 5 0.0 6 5 141 7 0.0 6 7 142 103 0.0 6 103 143 307 0.0 6 307 144 10 0.0 6 10 145 4 0.0 6 4 146 19 0.0 6 19 147 54 0.0 6 54 148 7 0.0 6 7 149 29 0.0 6 29 150 55 0.0 6 55 151 1 0.0 6 1 157 9 0.0 6 9 158 5 0.0 6 5 162 1 0.0 6 1 163 1 0.0 6 1 164 4 0.0 6 4 165 5 0.0 6 5 166 50 0.0 6 50 167 142 0.0 6 142 168 8 0.0 6 8 169 1 0.0 6 1 170 1 0.0 6 1 171 9 0.0 6 9 173 9 0.0 6 8 0 0 0 0 0 1 174 21 0.0 6 21 177 1 0.0 6 0 0 0 0 0 0 1 179 1 0.0 6 1 180 4 0.0 6 4 181 14 0.0 6 14 182 129 0.0 6 129 183 359 0.0 6 359 184 23 0.0 6 22 1 186 1 0.0 6 1 187 1 0.0 6 1 189 12 0.0 6 12 190 17 0.0 6 17 194 3 0.0 6 3 195 7 0.0 6 7 200 7 0.0 6 7 201 4 0.0 6 4 204 7 0.0 6 7 205 46 0.0 6 46 206 127 0.0 6 127 207 9 0.0 6 9 208 1 0.0 6 1 213 1 0.0 6 1 214 3 0.0 6 3 215 56 0.0 6 56 216 107 0.0 6 107 217 8 0.0 6 7 1 222 1 0.0 6 1 223 4 0.0 6 4 224 30 0.0 6 30 225 84 0.0 6 84 228 1 0.0 6 1 229 4 0.0 6 4 244 1 0.0 6 1 251 1 0.0 6 1 253 3 0.0 6 3 271 1 0.0 6 1 275 1 0.0 6 1 291 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 108735 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1768.9 0 1 1 9 1 0.4 2 1 10 10 0.1 3 8 2 11 6 0.0 3 6 12 5 0.0 3 4 1 13 11 0.0 3 11 14 4 0.0 4 3 1 16 10 0.0 4 9 0 0 1 17 25 0.0 5 19 5 1 18 27 0.0 5 20 7 19 16 0.0 5 15 1 20 131 0.0 6 126 5 21 82 0.0 6 78 4 22 45 0.0 6 43 2 23 23 0.0 6 23 24 42 0.0 6 40 2 25 38 0.0 6 36 2 26 29 0.0 6 28 1 27 19 0.0 6 19 28 34 0.0 6 32 2 29 25 0.0 6 20 5 30 18 0.0 6 17 1 31 15 0.0 6 14 1 32 12 0.0 6 11 1 33 7 0.0 6 3 3 1 34 9 0.0 6 9 35 15 0.0 6 14 1 36 14 0.0 6 12 1 1 37 18 0.0 6 17 1 38 8 0.0 6 6 1 0 0 0 0 1 39 12 0.0 6 12 40 11 0.0 6 11 41 16 0.0 6 15 0 1 42 18 0.0 6 15 3 43 12 0.0 6 12 44 5 0.0 6 5 45 15 0.0 6 14 0 0 0 0 0 1 46 15 0.0 6 15 47 13 0.0 6 11 1 1 48 8 0.0 6 7 1 49 20 0.0 6 16 3 0 0 1 50 15 0.0 6 14 1 51 15 0.0 6 12 3 52 15 0.0 6 13 2 53 19 0.0 6 17 1 0 1 54 17 0.0 6 17 55 27 0.0 6 24 2 0 1 56 29 0.0 6 27 2 57 24 0.0 6 21 3 58 33 0.0 6 29 1 1 0 1 1 59 22 0.0 6 20 2 60 18 0.0 6 12 4 1 1 61 22 0.0 6 18 4 62 16 0.0 6 13 3 63 20 0.0 6 18 1 0 1 64 27 0.0 6 24 2 0 1 65 25 0.0 6 23 2 66 46 0.0 6 44 2 67 197 0.0 6 186 8 1 1 1 68 54 0.0 6 49 3 1 0 0 0 1 69 104 0.0 6 98 4 0 0 2 70 37 0.0 6 34 2 0 0 1 71 69 0.0 6 68 1 72 44 0.0 6 40 4 73 27 0.0 6 25 1 0 0 1 74 35 0.0 6 35 75 36 0.0 6 35 1 76 32 0.0 6 30 1 0 0 1 77 34 0.0 6 29 4 1 78 35 0.0 6 34 1 79 31 0.0 6 31 80 50 0.0 6 48 1 0 0 0 0 1 81 47 0.0 6 43 2 1 0 0 0 1 82 42 0.0 6 39 3 83 63 0.0 6 57 3 2 0 0 0 1 84 30 0.0 6 29 1 85 33 0.0 6 32 0 0 0 1 86 29 0.0 6 27 1 0 1 87 36 0.0 6 31 5 88 49 0.0 6 46 2 1 89 34 0.0 6 34 90 57 0.0 6 52 4 0 0 1 91 60 0.0 6 56 3 1 92 66 0.0 6 62 1 1 0 0 0 2 93 66 0.0 6 62 3 0 0 1 94 40 0.0 6 36 4 95 56 0.0 6 55 1 96 71 0.0 6 69 1 0 0 1 97 68 0.0 6 65 3 98 65 0.0 6 60 4 1 99 56 0.0 6 55 1 100 49 0.0 6 48 1 101 44 0.0 6 41 3 102 73 0.0 6 72 1 103 89 0.0 6 89 104 126 0.0 6 124 2 105 89 0.0 6 88 1 106 96 0.0 6 93 3 107 87 0.0 6 85 2 108 94 0.0 6 92 2 109 79 0.0 6 77 2 110 125 0.0 6 120 5 111 81 0.0 6 79 2 112 100 0.0 6 98 1 1 113 115 0.0 6 112 3 114 124 0.0 6 123 1 115 136 0.0 6 134 2 116 130 0.0 6 128 1 0 0 1 117 148 0.0 6 146 2 118 165 0.0 6 162 3 119 142 0.0 6 141 1 120 154 0.0 6 151 2 1 121 128 0.0 6 126 2 122 136 0.0 6 135 0 0 0 1 123 132 0.0 6 132 124 186 0.0 6 182 3 0 1 125 189 0.0 6 186 3 126 182 0.0 6 179 3 127 222 0.0 6 222 128 189 0.0 6 188 1 129 260 0.0 6 254 4 1 1 130 237 0.0 6 231 5 1 131 257 0.0 6 253 4 132 378 0.0 6 375 3 133 353 0.0 6 348 4 1 134 451 0.0 6 447 4 135 369 0.0 6 364 3 0 1 1 136 466 0.0 6 457 8 0 0 1 137 373 0.0 6 372 1 138 473 0.0 6 470 1 1 0 1 139 568 0.0 6 559 7 0 0 1 1 140 473 0.0 6 468 3 0 1 1 141 731 0.0 6 725 4 0 1 1 142 1035 0.0 6 1025 5 1 2 2 143 1449 0.0 6 1440 8 0 0 1 144 1856 0.0 6 1846 7 0 1 2 145 1154 0.0 6 1133 13 1 1 6 146 1685 0.0 6 1662 19 0 2 2 147 1049 0.0 6 1043 4 0 0 2 148 1214 0.0 6 1202 8 0 0 4 149 1078 0.0 6 1065 13 150 1918 0.0 6 1908 7 0 1 2 151 1258 0.0 6 1251 6 0 0 0 1 152 2071 0.0 6 2065 5 0 0 1 153 1409 0.0 6 1401 8 154 1068 0.0 6 1061 5 1 0 1 155 1411 0.0 6 1405 6 156 1429 0.0 6 1423 5 0 1 157 1785 0.0 6 1776 9 158 1561 0.0 6 1548 12 1 159 2031 0.0 6 2021 9 0 1 160 1449 0.0 6 1444 5 161 1132 0.0 6 1125 7 162 1183 0.0 6 1175 7 1 163 1621 0.0 6 1617 4 164 2092 0.0 6 2084 8 165 1689 0.0 6 1679 9 1 166 1733 0.0 6 1723 10 167 2224 0.0 6 2218 6 168 1625 0.0 6 1615 8 0 1 0 0 1 169 1690 0.0 6 1678 11 0 0 1 170 1583 0.0 6 1576 5 0 1 0 0 1 171 1858 0.0 6 1847 7 2 1 0 1 172 2639 0.0 6 2629 7 0 2 1 173 3173 0.0 6 3166 5 0 0 1 0 1 174 2309 0.0 6 2302 5 1 1 175 1533 0.0 6 1529 4 176 2150 0.0 6 2142 7 0 0 1 177 1224 0.0 6 1215 7 1 0 1 178 1320 0.0 6 1309 10 1 179 1911 0.0 6 1907 3 0 1 180 1773 0.0 6 1772 1 181 1951 0.0 6 1942 8 1 182 681 0.0 6 677 3 0 0 0 1 183 2026 0.0 6 2018 7 0 0 1 184 2395 0.0 6 2393 2 185 2020 0.0 6 2016 3 0 0 1 186 1860 0.0 6 1850 8 2 187 1255 0.0 6 1248 6 1 188 1119 0.0 6 1107 9 2 0 1 189 1204 0.0 6 1198 6 190 1162 0.0 6 1149 11 2 191 1100 0.0 6 1094 6 192 914 0.0 6 911 2 0 1 193 689 0.0 6 688 1 194 1045 0.0 6 1043 2 195 622 0.0 6 618 4 196 693 0.0 6 688 5 197 484 0.0 6 483 1 198 400 0.0 6 396 4 199 605 0.0 6 599 5 0 0 1 200 532 0.0 6 531 1 201 414 0.0 6 412 1 0 0 0 1 202 514 0.0 6 512 2 203 451 0.0 6 451 204 610 0.0 6 608 2 205 458 0.0 6 456 2 206 588 0.0 6 585 3 207 431 0.0 6 431 208 333 0.0 6 331 1 0 0 0 1 209 441 0.0 6 440 1 210 228 0.0 6 228 211 584 0.0 6 583 1 212 407 0.0 6 405 2 213 212 0.0 6 211 0 1 214 424 0.0 6 422 2 215 360 0.0 6 358 2 216 252 0.0 6 251 1 217 299 0.0 6 299 218 241 0.0 6 241 219 246 0.0 6 243 3 220 202 0.0 6 201 1 221 287 0.0 6 285 2 222 149 0.0 6 147 2 223 219 0.0 6 215 4 224 146 0.0 6 145 1 225 144 0.0 6 143 1 226 138 0.0 6 138 227 74 0.0 6 72 2 228 49 0.0 6 49 229 74 0.0 6 74 230 53 0.0 6 53 231 48 0.0 6 48 232 52 0.0 6 52 233 44 0.0 6 44 234 53 0.0 6 52 1 235 21 0.0 6 21 236 13 0.0 6 13 237 12 0.0 6 12 238 8 0.0 6 8 239 24 0.0 6 24 240 30 0.0 6 29 0 0 0 1 241 21 0.0 6 21 242 10 0.0 6 10 243 10 0.0 6 10 244 9 0.0 6 9 245 2 0.0 6 2 246 3 0.0 6 3 247 6 0.0 6 6 248 5 0.0 6 5 249 4 0.0 6 4 250 1 0.0 6 1 251 3 0.0 6 2 1 252 1 0.0 6 1 277 3 0.0 6 3 278 17 0.0 6 17 279 189 0.0 6 188 1 280 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 4413 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 49 1 0.0 6 0 0 1 53 1 0.0 6 1 54 1 0.0 6 1 62 1 0.0 6 1 71 5 0.0 6 5 73 17 0.0 6 17 74 79 0.0 6 79 75 73 0.0 6 73 76 8 0.0 6 8 77 71 0.0 6 71 78 1 0.0 6 0 0 0 0 1 79 1 0.0 6 0 0 0 0 1 81 1 0.0 6 1 82 8 0.0 6 8 83 54 0.0 6 54 86 2 0.0 6 2 87 2 0.0 6 1 0 0 0 1 88 6 0.0 6 6 89 20 0.0 6 20 90 75 0.0 6 75 91 2 0.0 6 2 93 4 0.0 6 4 94 24 0.0 6 24 95 150 0.0 6 150 96 67 0.0 6 67 97 33 0.0 6 32 0 0 1 98 119 0.0 6 119 99 4 0.0 6 4 100 3 0.0 6 2 0 0 0 1 101 5 0.0 6 5 102 32 0.0 6 32 103 165 0.0 6 163 2 104 169 0.0 6 168 0 1 105 181 0.0 6 180 1 106 54 0.0 6 53 0 0 1 107 137 0.0 6 136 0 0 0 1 108 4 0.0 6 3 0 0 0 1 109 2 0.0 6 2 110 30 0.0 6 30 111 121 0.0 6 120 0 0 0 1 112 69 0.0 6 69 113 4 0.0 6 4 115 7 0.0 6 4 0 0 0 3 116 25 0.0 6 25 117 205 0.0 6 205 118 10 0.0 6 10 119 2 0.0 6 2 120 40 0.0 6 38 0 0 2 121 168 0.0 6 165 1 0 0 2 122 2 0.0 6 1 1 124 1 0.0 6 1 129 1 0.0 6 1 132 2 0.0 6 2 134 3 0.0 6 3 135 8 0.0 6 6 1 0 0 1 136 57 0.0 6 57 137 276 0.0 6 276 138 8 0.0 6 7 1 139 1 0.0 6 1 140 5 0.0 6 4 1 141 65 0.0 6 64 0 0 0 1 142 342 0.0 6 342 143 10 0.0 6 9 1 144 1 0.0 6 1 145 16 0.0 6 15 0 0 0 1 146 83 0.0 6 83 147 4 0.0 6 4 148 20 0.0 6 20 149 94 0.0 6 94 150 3 0.0 6 3 152 1 0.0 6 0 0 0 0 1 155 2 0.0 6 2 156 5 0.0 6 5 157 5 0.0 6 5 161 1 0.0 6 1 162 1 0.0 6 1 163 2 0.0 6 2 165 26 0.0 6 25 1 166 151 0.0 6 151 167 2 0.0 6 2 168 1 0.0 6 1 169 1 0.0 6 1 170 7 0.0 6 7 171 1 0.0 6 1 172 7 0.0 6 7 173 28 0.0 6 28 174 3 0.0 6 2 1 176 1 0.0 6 0 0 0 0 1 179 2 0.0 6 2 180 11 0.0 6 11 181 74 0.0 6 73 1 182 386 0.0 6 385 1 183 7 0.0 6 7 184 3 0.0 6 2 0 1 185 1 0.0 6 0 0 0 0 1 188 8 0.0 6 8 189 16 0.0 6 16 193 1 0.0 6 1 194 15 0.0 6 15 195 1 0.0 6 1 198 1 0.0 6 1 200 3 0.0 6 3 203 5 0.0 6 5 204 31 0.0 6 31 205 134 0.0 6 134 206 10 0.0 6 10 207 1 0.0 6 0 0 0 0 1 209 2 0.0 6 0 0 0 0 2 212 1 0.0 6 1 213 1 0.0 6 1 214 20 0.0 6 20 215 93 0.0 6 93 216 3 0.0 6 3 218 2 0.0 6 1 0 0 0 1 219 2 0.0 6 0 0 0 0 2 222 2 0.0 6 2 223 11 0.0 6 11 224 54 0.0 6 54 225 1 0.0 6 1 227 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_16_127_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_16_354_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_16_127_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_16_354_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 6.39 s (53 µs/read; 1.12 M reads/minute). === Summary === Total read pairs processed: 119,849 Read 1 with adapter: 119,844 (100.0%) Read 2 with adapter: 119,813 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 41 (0.0%) Pairs written (passing filters): 119,808 (100.0%) Total basepairs processed: 63,246,717 bp Read 1: 34,953,227 bp Read 2: 28,293,490 bp Total written (filtered): 7,548,815 bp (11.9%) Read 1: 3,952,999 bp Read 2: 3,595,816 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 119821 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1872.6 0 0 2 5 3 117.0 1 3 6 4 29.3 1 0 0 4 7 3 7.3 2 1 0 2 9 3 0.5 2 2 1 15 3 0.0 4 3 16 2 0.0 4 1 0 1 17 1 0.0 5 1 19 1 0.0 5 1 20 22 0.0 6 22 21 11 0.0 6 11 22 10 0.0 6 9 1 23 11 0.0 6 9 2 24 9 0.0 6 9 25 2 0.0 6 1 1 26 2 0.0 6 1 1 27 4 0.0 6 4 29 1 0.0 6 0 1 30 2 0.0 6 2 31 7 0.0 6 7 32 1 0.0 6 1 33 4 0.0 6 4 34 3 0.0 6 3 36 2 0.0 6 1 1 37 4 0.0 6 4 38 8 0.0 6 8 39 4 0.0 6 4 40 8 0.0 6 7 1 41 5 0.0 6 5 42 3 0.0 6 3 43 2 0.0 6 2 44 6 0.0 6 6 45 107 0.0 6 106 1 46 17 0.0 6 17 47 7 0.0 6 7 48 8 0.0 6 8 49 2 0.0 6 2 50 6 0.0 6 6 51 2 0.0 6 2 52 2 0.0 6 2 53 3 0.0 6 3 54 11 0.0 6 11 55 3 0.0 6 3 56 1 0.0 6 1 57 9 0.0 6 8 1 58 9 0.0 6 7 2 59 3 0.0 6 3 60 4 0.0 6 4 61 4 0.0 6 4 62 1 0.0 6 1 63 4 0.0 6 4 64 1 0.0 6 1 65 10 0.0 6 10 66 2 0.0 6 2 67 2 0.0 6 2 68 5 0.0 6 5 69 9 0.0 6 8 1 70 3 0.0 6 3 73 1 0.0 6 1 74 6 0.0 6 6 75 2 0.0 6 2 76 4 0.0 6 4 77 1 0.0 6 1 78 2 0.0 6 1 1 79 4 0.0 6 4 80 2 0.0 6 2 82 3 0.0 6 3 83 2 0.0 6 2 84 1 0.0 6 1 86 1 0.0 6 0 1 87 20 0.0 6 20 88 7 0.0 6 7 90 2 0.0 6 2 91 2 0.0 6 2 92 1 0.0 6 1 93 2 0.0 6 2 94 1 0.0 6 1 96 1 0.0 6 1 98 2 0.0 6 2 99 4 0.0 6 4 100 1 0.0 6 1 101 3 0.0 6 3 102 6 0.0 6 4 1 0 1 103 2 0.0 6 2 104 1 0.0 6 1 105 7 0.0 6 7 106 3 0.0 6 3 107 7 0.0 6 7 108 8 0.0 6 8 109 3 0.0 6 2 1 110 2 0.0 6 2 111 7 0.0 6 6 1 112 8 0.0 6 8 113 6 0.0 6 6 114 4 0.0 6 3 1 115 15 0.0 6 14 1 116 1 0.0 6 1 117 2 0.0 6 1 1 118 3 0.0 6 1 2 119 9 0.0 6 9 120 6 0.0 6 5 1 121 12 0.0 6 12 122 21 0.0 6 21 123 13 0.0 6 12 0 1 124 13 0.0 6 13 125 6 0.0 6 6 126 14 0.0 6 14 127 28 0.0 6 28 128 35 0.0 6 34 1 129 20 0.0 6 20 130 16 0.0 6 16 131 11 0.0 6 7 4 132 19 0.0 6 18 1 133 49 0.0 6 47 1 1 134 31 0.0 6 28 3 135 14 0.0 6 14 136 16 0.0 6 15 1 137 4 0.0 6 4 138 23 0.0 6 21 2 139 46 0.0 6 44 2 140 21 0.0 6 20 1 141 137 0.0 6 136 1 142 49 0.0 6 49 143 47 0.0 6 45 2 144 70 0.0 6 70 145 41 0.0 6 39 1 0 1 146 42 0.0 6 36 6 147 80 0.0 6 80 148 65 0.0 6 63 2 149 34 0.0 6 32 1 1 150 36 0.0 6 35 1 151 22 0.0 6 22 152 31 0.0 6 30 1 153 57 0.0 6 57 154 32 0.0 6 30 2 155 14 0.0 6 14 156 9 0.0 6 9 157 16 0.0 6 16 158 74 0.0 6 73 1 159 33 0.0 6 31 2 160 36 0.0 6 35 1 161 54 0.0 6 52 2 162 38 0.0 6 37 1 163 33 0.0 6 30 3 164 14 0.0 6 13 1 165 41 0.0 6 38 3 166 37 0.0 6 37 167 38 0.0 6 35 3 168 34 0.0 6 32 2 169 19 0.0 6 19 170 17 0.0 6 16 1 171 28 0.0 6 26 2 172 25 0.0 6 25 173 36 0.0 6 36 174 33 0.0 6 32 1 175 31 0.0 6 28 3 176 15 0.0 6 14 0 1 177 34 0.0 6 33 1 178 32 0.0 6 31 1 179 52 0.0 6 49 3 180 42 0.0 6 41 1 181 65 0.0 6 64 1 182 83 0.0 6 81 2 183 60 0.0 6 56 4 184 47 0.0 6 46 1 185 105 0.0 6 102 3 186 94 0.0 6 90 4 187 91 0.0 6 89 2 188 147 0.0 6 147 189 97 0.0 6 93 3 1 190 75 0.0 6 74 1 191 51 0.0 6 48 3 192 35 0.0 6 34 1 193 73 0.0 6 73 194 78 0.0 6 78 195 88 0.0 6 86 1 1 196 134 0.0 6 131 3 197 80 0.0 6 79 1 198 94 0.0 6 92 1 1 199 164 0.0 6 159 5 200 133 0.0 6 123 9 0 1 201 168 0.0 6 163 5 202 172 0.0 6 164 8 203 121 0.0 6 117 4 204 130 0.0 6 123 6 0 0 1 205 242 0.0 6 235 7 206 327 0.0 6 315 12 207 850 0.0 6 826 23 1 208 545 0.0 6 524 20 0 1 209 441 0.0 6 428 11 2 210 779 0.0 6 750 28 1 211 737 0.0 6 713 23 1 212 545 0.0 6 526 18 1 213 372 0.0 6 362 10 214 424 0.0 6 412 11 0 0 0 1 215 194 0.0 6 188 6 216 283 0.0 6 274 9 217 222 0.0 6 215 5 2 218 340 0.0 6 332 8 219 244 0.0 6 235 9 220 315 0.0 6 300 14 1 221 423 0.0 6 410 13 222 608 0.0 6 592 16 223 391 0.0 6 379 12 224 183 0.0 6 176 7 225 270 0.0 6 264 6 226 211 0.0 6 204 7 227 493 0.0 6 481 12 228 384 0.0 6 375 9 229 184 0.0 6 177 7 230 369 0.0 6 357 12 231 650 0.0 6 639 10 1 232 268 0.0 6 262 6 233 303 0.0 6 298 5 234 385 0.0 6 374 9 1 0 0 0 1 235 214 0.0 6 200 14 236 748 0.0 6 738 9 0 0 0 0 1 237 207 0.0 6 193 14 238 659 0.0 6 645 13 1 239 397 0.0 6 392 4 1 240 159 0.0 6 155 4 241 292 0.0 6 285 7 242 261 0.0 6 249 12 243 298 0.0 6 283 13 1 0 1 244 559 0.0 6 542 16 1 245 761 0.0 6 739 20 0 2 246 541 0.0 6 529 12 247 331 0.0 6 310 21 248 1158 0.0 6 1131 24 2 0 1 249 694 0.0 6 668 25 1 250 2432 0.0 6 2385 47 251 829 0.0 6 816 13 252 423 0.0 6 401 21 1 253 1100 0.0 6 1056 42 2 254 1371 0.0 6 1307 62 2 255 2422 0.0 6 2371 48 3 256 2170 0.0 6 2108 61 1 257 3434 0.0 6 3386 45 3 258 1425 0.0 6 1372 47 6 259 3398 0.0 6 3312 83 3 260 1597 0.0 6 1541 55 1 261 3021 0.0 6 2951 68 1 0 1 262 1368 0.0 6 1330 38 263 883 0.0 6 858 24 1 264 1783 0.0 6 1722 60 0 0 1 265 2263 0.0 6 2216 46 1 266 961 0.0 6 928 30 2 1 267 2007 0.0 6 1963 41 2 1 268 1175 0.0 6 1129 44 2 269 1741 0.0 6 1693 47 0 0 1 270 669 0.0 6 650 17 1 1 271 957 0.0 6 941 15 1 272 885 0.0 6 856 26 2 1 273 1642 0.0 6 1572 68 2 274 5404 0.0 6 5257 141 4 2 275 3858 0.0 6 3719 130 6 1 2 276 5594 0.0 6 5405 184 5 277 39817 0.0 6 38784 1006 22 2 2 1 278 802 0.0 6 629 168 4 0 1 279 179 0.0 6 168 5 4 2 280 5 0.0 6 2 1 0 1 0 1 281 5 0.0 6 2 1 0 0 2 282 2 0.0 6 0 1 0 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 23 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 73 3 0.0 6 3 75 3 0.0 6 3 79 2 0.0 6 2 83 1 0.0 6 1 84 2 0.0 6 2 85 2 0.0 6 2 90 1 0.0 6 1 103 1 0.0 6 1 120 1 0.0 6 1 127 1 0.0 6 1 128 1 0.0 6 1 131 1 0.0 6 1 138 1 0.0 6 1 154 1 0.0 6 1 155 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 119781 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 117.0 1 1 6 3 29.3 1 2 1 7 1 7.3 2 0 1 10 1 0.1 3 1 11 5 0.0 3 5 12 7 0.0 3 7 13 13 0.0 3 11 2 14 1 0.0 4 1 15 3 0.0 4 3 16 3 0.0 4 3 17 5 0.0 5 4 1 18 7 0.0 5 3 3 1 19 9 0.0 5 9 20 43 0.0 6 39 3 1 21 40 0.0 6 39 1 22 39 0.0 6 39 23 16 0.0 6 15 0 1 24 8 0.0 6 8 25 14 0.0 6 12 2 26 8 0.0 6 7 1 27 10 0.0 6 10 28 9 0.0 6 9 29 13 0.0 6 12 1 30 15 0.0 6 13 2 31 10 0.0 6 9 1 32 7 0.0 6 7 33 4 0.0 6 4 34 7 0.0 6 6 1 35 10 0.0 6 9 1 36 5 0.0 6 5 37 9 0.0 6 8 1 38 16 0.0 6 16 39 11 0.0 6 11 40 10 0.0 6 9 1 41 11 0.0 6 10 1 42 4 0.0 6 4 43 7 0.0 6 7 44 17 0.0 6 13 3 0 0 1 45 5 0.0 6 3 2 46 14 0.0 6 13 1 47 20 0.0 6 17 3 48 11 0.0 6 10 1 49 20 0.0 6 19 1 50 11 0.0 6 10 1 51 9 0.0 6 8 1 52 15 0.0 6 15 53 4 0.0 6 4 54 12 0.0 6 11 1 55 23 0.0 6 23 56 21 0.0 6 19 0 1 1 57 20 0.0 6 19 1 58 17 0.0 6 17 59 15 0.0 6 14 1 60 14 0.0 6 10 4 61 4 0.0 6 2 2 62 6 0.0 6 6 63 11 0.0 6 9 2 64 13 0.0 6 13 65 16 0.0 6 13 3 66 20 0.0 6 19 1 67 15 0.0 6 12 3 68 26 0.0 6 25 1 69 191 0.0 6 178 12 1 70 32 0.0 6 31 1 71 97 0.0 6 92 4 1 72 55 0.0 6 53 2 73 22 0.0 6 22 74 21 0.0 6 19 2 75 15 0.0 6 13 2 76 42 0.0 6 39 2 1 77 33 0.0 6 32 0 1 78 44 0.0 6 43 1 79 33 0.0 6 32 1 80 44 0.0 6 38 5 1 81 25 0.0 6 24 1 82 21 0.0 6 20 1 83 37 0.0 6 34 3 84 82 0.0 6 78 2 0 0 2 85 43 0.0 6 39 4 86 16 0.0 6 13 3 87 13 0.0 6 13 88 3 0.0 6 3 89 25 0.0 6 23 2 90 28 0.0 6 25 2 0 0 1 91 20 0.0 6 18 1 1 92 25 0.0 6 23 1 0 0 1 93 43 0.0 6 38 3 2 94 68 0.0 6 62 5 1 95 34 0.0 6 29 5 96 33 0.0 6 31 2 97 120 0.0 6 113 7 98 43 0.0 6 41 2 99 22 0.0 6 20 1 1 100 42 0.0 6 41 0 0 0 0 1 101 46 0.0 6 44 2 102 55 0.0 6 53 2 103 76 0.0 6 69 6 1 104 86 0.0 6 81 5 105 99 0.0 6 95 4 106 36 0.0 6 33 3 107 78 0.0 6 72 6 108 30 0.0 6 27 3 109 14 0.0 6 13 1 110 33 0.0 6 33 111 9 0.0 6 9 112 9 0.0 6 9 113 23 0.0 6 23 114 29 0.0 6 26 3 115 66 0.0 6 60 6 116 74 0.0 6 68 5 0 0 1 117 127 0.0 6 120 5 1 0 1 118 44 0.0 6 43 1 119 118 0.0 6 110 4 4 120 54 0.0 6 46 7 0 0 1 121 92 0.0 6 85 7 122 66 0.0 6 61 3 2 123 165 0.0 6 151 11 2 0 1 124 75 0.0 6 72 3 125 89 0.0 6 88 1 126 127 0.0 6 118 9 127 212 0.0 6 196 14 2 128 73 0.0 6 64 8 1 129 144 0.0 6 138 6 130 148 0.0 6 141 6 0 1 131 135 0.0 6 128 6 0 1 132 46 0.0 6 42 4 133 55 0.0 6 53 2 134 87 0.0 6 86 1 135 153 0.0 6 143 9 0 1 136 157 0.0 6 152 5 137 105 0.0 6 101 4 138 256 0.0 6 236 19 1 139 154 0.0 6 148 6 140 117 0.0 6 113 4 141 309 0.0 6 297 10 1 0 1 142 155 0.0 6 143 12 143 274 0.0 6 260 13 0 0 1 144 238 0.0 6 230 6 0 0 2 145 97 0.0 6 90 6 0 0 1 146 245 0.0 6 233 12 147 219 0.0 6 212 6 1 148 324 0.0 6 310 13 1 149 212 0.0 6 202 8 1 0 1 150 256 0.0 6 252 4 151 259 0.0 6 248 11 152 337 0.0 6 328 8 1 153 269 0.0 6 256 13 154 375 0.0 6 355 18 1 0 1 155 906 0.0 6 868 36 1 1 156 1245 0.0 6 1197 48 157 855 0.0 6 824 27 0 0 4 158 333 0.0 6 324 8 0 0 1 159 437 0.0 6 423 13 0 1 160 212 0.0 6 197 13 1 0 1 161 251 0.0 6 232 18 0 1 162 335 0.0 6 323 11 1 163 510 0.0 6 482 26 0 1 1 164 1102 0.0 6 1054 44 2 0 2 165 477 0.0 6 450 25 0 1 1 166 809 0.0 6 777 29 1 1 1 167 405 0.0 6 389 13 2 0 1 168 244 0.0 6 233 11 169 366 0.0 6 351 13 1 0 0 0 1 170 563 0.0 6 547 14 1 0 0 1 171 600 0.0 6 570 30 172 960 0.0 6 925 31 2 0 2 173 432 0.0 6 409 22 1 174 1127 0.0 6 1089 35 3 175 1009 0.0 6 977 31 0 0 0 0 1 176 356 0.0 6 343 11 1 0 1 177 326 0.0 6 311 13 2 178 374 0.0 6 344 29 0 1 179 562 0.0 6 538 21 2 0 0 1 180 290 0.0 6 277 10 1 2 181 426 0.0 6 413 10 1 2 182 319 0.0 6 303 15 1 183 557 0.0 6 537 19 1 184 201 0.0 6 188 11 0 1 1 185 684 0.0 6 653 29 1 1 186 436 0.0 6 413 21 0 0 1 0 1 187 1262 0.0 6 1213 45 2 1 1 188 824 0.0 6 786 32 2 1 2 0 1 189 1187 0.0 6 1146 40 1 190 498 0.0 6 468 28 1 0 1 191 827 0.0 6 792 34 0 0 1 192 1107 0.0 6 1066 40 1 193 1335 0.0 6 1303 31 0 1 194 1269 0.0 6 1214 53 1 0 1 195 997 0.0 6 964 31 1 0 1 196 635 0.0 6 605 29 1 197 435 0.0 6 416 16 3 198 1567 0.0 6 1516 48 3 199 1780 0.0 6 1718 60 2 200 1780 0.0 6 1723 54 1 0 1 1 201 1254 0.0 6 1199 51 4 202 1515 0.0 6 1470 41 2 0 1 1 203 1186 0.0 6 1143 40 2 1 204 1092 0.0 6 1060 30 1 1 205 911 0.0 6 882 26 1 1 1 206 1472 0.0 6 1432 38 1 1 207 251 0.0 6 235 15 1 208 937 0.0 6 901 33 0 0 3 209 1097 0.0 6 1057 37 1 0 2 210 620 0.0 6 594 23 2 1 211 127 0.0 6 117 7 0 3 212 388 0.0 6 370 17 0 0 1 213 95 0.0 6 90 4 0 1 214 345 0.0 6 332 13 215 303 0.0 6 288 14 0 0 1 216 960 0.0 6 925 33 2 217 685 0.0 6 659 24 2 218 1135 0.0 6 1102 33 219 1183 0.0 6 1152 29 1 0 1 220 459 0.0 6 438 18 2 1 221 393 0.0 6 370 20 1 2 222 505 0.0 6 469 27 8 1 223 2274 0.0 6 2132 136 3 0 3 224 7621 0.0 6 7366 236 8 2 8 1 225 2826 0.0 6 2726 80 14 0 5 1 226 2760 0.0 6 2609 146 5 227 12074 0.0 6 11731 328 3 1 10 1 228 1525 0.0 6 1440 81 2 1 1 229 4005 0.0 6 3872 126 4 0 3 230 705 0.0 6 673 24 6 1 1 231 3869 0.0 6 3734 126 5 0 4 232 2282 0.0 6 2215 59 6 1 1 233 3001 0.0 6 2899 98 3 1 234 4172 0.0 6 4048 114 5 0 5 235 745 0.0 6 725 18 1 1 236 357 0.0 6 347 8 0 1 1 237 389 0.0 6 379 10 238 433 0.0 6 420 12 0 0 1 239 148 0.0 6 144 4 240 158 0.0 6 147 11 241 977 0.0 6 951 25 1 242 234 0.0 6 226 7 1 243 1060 0.0 6 1029 29 2 244 1758 0.0 6 1711 45 0 0 1 1 245 215 0.0 6 207 7 0 1 246 662 0.0 6 644 18 247 278 0.0 6 270 7 0 0 1 248 647 0.0 6 625 19 1 0 2 249 1225 0.0 6 1195 29 1 250 335 0.0 6 319 16 251 454 0.0 6 436 18 252 887 0.0 6 852 33 1 1 253 331 0.0 6 322 8 1 254 776 0.0 6 758 18 255 502 0.0 6 492 10 256 274 0.0 6 270 4 257 95 0.0 6 87 8 258 12 0.0 6 12 259 2 0.0 6 2 260 4 0.0 6 4 261 3 0.0 6 3 262 6 0.0 6 6 263 13 0.0 6 13 264 3 0.0 6 3 265 5 0.0 6 5 266 8 0.0 6 8 267 2 0.0 6 2 268 5 0.0 6 5 269 4 0.0 6 4 271 3 0.0 6 3 272 2 0.0 6 2 277 3 0.0 6 3 278 2 0.0 6 1 1 279 25 0.0 6 24 1 280 85 0.0 6 81 3 1 281 160 0.0 6 147 11 1 0 1 282 8 0.0 6 3 5 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 32 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 57 6 0.0 6 6 69 2 0.0 6 2 70 2 0.0 6 2 71 1 0.0 6 1 72 3 0.0 6 3 76 1 0.0 6 1 77 1 0.0 6 1 82 1 0.0 6 1 84 1 0.0 6 1 102 1 0.0 6 1 103 1 0.0 6 1 109 1 0.0 6 1 110 1 0.0 6 1 113 1 0.0 6 1 114 1 0.0 6 0 0 0 0 0 1 115 2 0.0 6 0 0 0 0 0 2 116 2 0.0 6 1 0 0 0 0 1 117 2 0.0 6 1 0 0 0 0 1 135 1 0.0 6 1 157 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_17_128_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_17_355_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_17_128_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_17_355_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.10 s (44 µs/read; 1.37 M reads/minute). === Summary === Total read pairs processed: 93,552 Read 1 with adapter: 93,538 (100.0%) Read 2 with adapter: 93,501 (99.9%) == Read fate breakdown == Pairs that were too short: 31 (0.0%) Pairs discarded as untrimmed: 65 (0.1%) Pairs written (passing filters): 93,456 (99.9%) Total basepairs processed: 38,082,524 bp Read 1: 20,838,077 bp Read 2: 17,244,447 bp Total written (filtered): 6,058,440 bp (15.9%) Read 1: 3,228,023 bp Read 2: 2,830,417 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 90796 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 1461.8 0 1 3 4 5 365.4 1 3 2 5 2 91.4 1 2 6 5 22.8 1 3 0 2 7 4 5.7 2 2 0 2 9 5 0.4 2 5 11 1 0.0 3 0 1 13 2 0.0 3 2 15 1 0.0 4 1 16 3 0.0 4 2 1 17 3 0.0 5 3 18 2 0.0 5 2 19 2 0.0 5 2 20 163 0.0 6 158 5 21 94 0.0 6 94 22 65 0.0 6 65 23 49 0.0 6 48 1 24 38 0.0 6 35 1 1 0 1 25 53 0.0 6 53 26 27 0.0 6 26 1 27 30 0.0 6 29 1 28 18 0.0 6 17 1 29 14 0.0 6 13 1 30 11 0.0 6 11 31 14 0.0 6 12 2 32 4 0.0 6 4 33 10 0.0 6 10 34 13 0.0 6 11 2 35 8 0.0 6 8 36 16 0.0 6 15 1 37 11 0.0 6 11 38 8 0.0 6 8 39 14 0.0 6 14 40 9 0.0 6 9 41 19 0.0 6 17 2 42 7 0.0 6 7 43 10 0.0 6 9 1 44 8 0.0 6 8 45 6 0.0 6 6 46 9 0.0 6 8 1 47 12 0.0 6 12 48 19 0.0 6 18 1 49 14 0.0 6 13 1 50 13 0.0 6 12 1 51 11 0.0 6 11 52 5 0.0 6 5 53 5 0.0 6 5 54 9 0.0 6 9 55 8 0.0 6 7 1 56 6 0.0 6 4 2 57 6 0.0 6 5 1 58 7 0.0 6 6 1 59 10 0.0 6 8 1 0 1 60 5 0.0 6 5 61 14 0.0 6 13 1 62 5 0.0 6 5 63 16 0.0 6 16 64 13 0.0 6 13 65 10 0.0 6 10 66 11 0.0 6 10 1 67 9 0.0 6 8 1 68 12 0.0 6 12 69 13 0.0 6 12 1 70 8 0.0 6 6 1 1 71 24 0.0 6 23 1 72 17 0.0 6 15 2 73 15 0.0 6 14 0 1 74 11 0.0 6 10 0 0 0 0 1 75 13 0.0 6 12 0 0 0 1 76 14 0.0 6 13 1 77 13 0.0 6 13 78 17 0.0 6 17 79 11 0.0 6 11 80 17 0.0 6 17 81 18 0.0 6 18 82 18 0.0 6 17 1 83 16 0.0 6 15 0 1 84 21 0.0 6 21 85 23 0.0 6 22 0 1 86 11 0.0 6 11 87 16 0.0 6 16 88 18 0.0 6 18 89 7 0.0 6 7 90 7 0.0 6 7 91 18 0.0 6 17 1 92 9 0.0 6 9 93 20 0.0 6 20 94 19 0.0 6 19 95 12 0.0 6 12 96 14 0.0 6 13 0 0 0 0 0 1 97 13 0.0 6 13 98 16 0.0 6 15 1 99 25 0.0 6 24 1 100 23 0.0 6 22 1 101 25 0.0 6 25 102 31 0.0 6 30 1 103 40 0.0 6 40 104 35 0.0 6 34 0 0 0 0 0 1 105 34 0.0 6 34 106 25 0.0 6 24 1 107 27 0.0 6 27 108 35 0.0 6 33 2 109 36 0.0 6 34 2 110 31 0.0 6 30 1 111 35 0.0 6 35 112 36 0.0 6 36 113 40 0.0 6 40 114 64 0.0 6 62 2 115 54 0.0 6 53 0 0 0 0 1 116 49 0.0 6 48 1 117 50 0.0 6 47 1 0 0 0 2 118 47 0.0 6 47 119 37 0.0 6 37 120 26 0.0 6 25 1 121 36 0.0 6 36 122 42 0.0 6 41 0 0 1 123 46 0.0 6 45 1 124 55 0.0 6 54 1 125 54 0.0 6 53 1 126 50 0.0 6 50 127 58 0.0 6 58 128 69 0.0 6 69 129 80 0.0 6 78 2 130 68 0.0 6 68 131 70 0.0 6 70 132 77 0.0 6 75 2 133 76 0.0 6 76 134 77 0.0 6 77 135 89 0.0 6 88 1 136 106 0.0 6 105 1 137 89 0.0 6 87 2 138 83 0.0 6 80 3 139 154 0.0 6 154 140 302 0.0 6 300 2 141 661 0.0 6 660 1 142 764 0.0 6 761 2 1 143 695 0.0 6 694 1 144 804 0.0 6 804 145 1056 0.0 6 1056 146 837 0.0 6 834 3 147 1671 0.0 6 1666 5 148 1366 0.0 6 1363 2 0 0 0 1 149 1301 0.0 6 1297 4 150 1515 0.0 6 1513 1 0 1 151 746 0.0 6 745 1 152 686 0.0 6 685 1 153 761 0.0 6 759 1 0 1 154 653 0.0 6 653 155 630 0.0 6 630 156 616 0.0 6 615 1 157 931 0.0 6 928 3 158 815 0.0 6 811 4 159 1058 0.0 6 1054 4 160 828 0.0 6 825 3 161 940 0.0 6 939 1 162 770 0.0 6 768 2 163 738 0.0 6 737 1 164 906 0.0 6 906 165 588 0.0 6 586 2 166 1071 0.0 6 1066 5 167 580 0.0 6 577 3 168 692 0.0 6 691 1 169 689 0.0 6 685 4 170 1029 0.0 6 1026 2 0 0 1 171 762 0.0 6 758 4 172 738 0.0 6 735 2 0 0 1 173 739 0.0 6 736 2 1 174 703 0.0 6 702 1 175 541 0.0 6 540 1 176 637 0.0 6 637 177 474 0.0 6 473 1 178 830 0.0 6 828 1 0 0 1 179 727 0.0 6 726 1 180 565 0.0 6 563 2 181 718 0.0 6 710 7 1 182 648 0.0 6 644 3 0 0 0 0 1 183 877 0.0 6 876 1 184 693 0.0 6 691 2 185 729 0.0 6 726 2 0 0 0 0 1 186 662 0.0 6 658 4 187 545 0.0 6 545 188 655 0.0 6 647 7 0 0 0 1 189 571 0.0 6 570 1 190 517 0.0 6 516 1 191 838 0.0 6 833 5 192 701 0.0 6 697 3 0 0 0 0 1 193 1150 0.0 6 1144 4 0 1 0 0 1 194 1243 0.0 6 1240 2 0 0 0 1 195 847 0.0 6 843 4 196 1234 0.0 6 1230 3 0 1 197 1268 0.0 6 1262 4 0 1 0 0 1 198 894 0.0 6 892 2 199 772 0.0 6 769 3 200 911 0.0 6 910 1 201 766 0.0 6 761 4 0 0 0 0 1 202 1307 0.0 6 1302 5 203 972 0.0 6 967 5 204 826 0.0 6 823 2 1 205 1207 0.0 6 1201 5 0 0 0 0 1 206 948 0.0 6 947 1 207 1055 0.0 6 1052 1 0 1 0 1 208 726 0.0 6 722 3 0 0 0 1 209 957 0.0 6 955 2 210 949 0.0 6 948 1 211 894 0.0 6 890 3 0 0 0 1 212 891 0.0 6 888 3 213 868 0.0 6 864 4 214 1025 0.0 6 1024 1 215 827 0.0 6 825 2 216 904 0.0 6 901 2 0 1 217 943 0.0 6 934 7 0 0 0 0 2 218 830 0.0 6 826 4 219 649 0.0 6 646 3 220 731 0.0 6 724 7 221 704 0.0 6 702 2 222 645 0.0 6 643 2 223 686 0.0 6 683 1 0 0 0 1 1 224 889 0.0 6 888 1 225 691 0.0 6 689 2 226 704 0.0 6 701 3 227 505 0.0 6 503 2 228 521 0.0 6 518 3 229 583 0.0 6 581 1 0 1 230 512 0.0 6 508 3 0 0 1 231 542 0.0 6 541 1 232 580 0.0 6 576 3 0 0 0 1 233 531 0.0 6 527 4 234 428 0.0 6 424 4 235 508 0.0 6 503 3 1 0 1 236 393 0.0 6 390 3 237 439 0.0 6 436 3 238 414 0.0 6 412 2 239 356 0.0 6 355 1 240 412 0.0 6 410 2 241 350 0.0 6 345 4 0 0 1 242 316 0.0 6 316 243 326 0.0 6 323 3 244 288 0.0 6 286 2 245 263 0.0 6 262 1 246 210 0.0 6 210 247 254 0.0 6 250 4 248 189 0.0 6 188 1 249 213 0.0 6 213 250 194 0.0 6 190 4 251 163 0.0 6 160 2 0 0 0 1 252 157 0.0 6 157 253 172 0.0 6 170 2 254 165 0.0 6 163 1 0 0 1 255 174 0.0 6 171 3 256 184 0.0 6 183 1 257 165 0.0 6 164 1 258 181 0.0 6 180 0 0 0 0 1 259 159 0.0 6 159 260 116 0.0 6 114 2 261 115 0.0 6 115 262 104 0.0 6 104 263 110 0.0 6 110 264 107 0.0 6 107 265 85 0.0 6 85 266 99 0.0 6 99 267 99 0.0 6 99 268 114 0.0 6 112 2 269 88 0.0 6 88 270 106 0.0 6 105 1 271 84 0.0 6 84 272 96 0.0 6 96 273 113 0.0 6 113 274 133 0.0 6 132 1 275 152 0.0 6 151 1 276 240 0.0 6 239 0 0 1 277 662 0.0 6 651 11 278 1201 0.0 6 1193 8 279 23 0.0 6 23 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2742 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 59 2 0.0 6 2 61 1 0.0 6 0 0 0 1 62 2 0.0 6 1 0 1 63 9 0.0 6 7 2 64 118 0.0 6 115 3 66 1 0.0 6 1 72 4 0.0 6 3 1 73 13 0.0 6 13 74 26 0.0 6 26 75 28 0.0 6 28 76 24 0.0 6 24 77 27 0.0 6 27 78 38 0.0 6 38 79 42 0.0 6 42 80 49 0.0 6 49 81 53 0.0 6 53 82 50 0.0 6 50 83 66 0.0 6 66 84 14 0.0 6 14 85 17 0.0 6 17 86 26 0.0 6 25 1 87 16 0.0 6 16 88 11 0.0 6 11 89 17 0.0 6 17 90 40 0.0 6 40 91 30 0.0 6 30 92 34 0.0 6 34 93 23 0.0 6 23 94 24 0.0 6 24 95 32 0.0 6 32 96 25 0.0 6 25 97 20 0.0 6 20 98 22 0.0 6 22 99 32 0.0 6 32 100 23 0.0 6 23 101 26 0.0 6 26 102 23 0.0 6 23 103 30 0.0 6 30 104 17 0.0 6 17 105 22 0.0 6 21 1 106 21 0.0 6 21 107 23 0.0 6 23 108 34 0.0 6 34 109 20 0.0 6 19 0 0 0 0 0 1 110 14 0.0 6 14 111 22 0.0 6 22 112 15 0.0 6 15 113 20 0.0 6 20 114 14 0.0 6 14 115 23 0.0 6 23 116 25 0.0 6 25 117 12 0.0 6 12 118 19 0.0 6 19 119 8 0.0 6 8 120 9 0.0 6 9 121 9 0.0 6 9 122 7 0.0 6 7 123 6 0.0 6 6 124 15 0.0 6 15 125 15 0.0 6 15 126 32 0.0 6 32 127 19 0.0 6 19 128 12 0.0 6 12 129 22 0.0 6 22 130 15 0.0 6 15 131 9 0.0 6 9 132 10 0.0 6 10 133 13 0.0 6 13 134 24 0.0 6 24 135 50 0.0 6 50 136 26 0.0 6 26 137 17 0.0 6 17 138 12 0.0 6 11 0 0 0 0 0 1 139 23 0.0 6 22 1 140 20 0.0 6 20 141 12 0.0 6 12 142 35 0.0 6 35 143 17 0.0 6 17 144 16 0.0 6 16 145 14 0.0 6 13 1 146 12 0.0 6 12 147 8 0.0 6 8 148 18 0.0 6 18 149 16 0.0 6 16 150 22 0.0 6 22 151 18 0.0 6 18 152 17 0.0 6 17 153 16 0.0 6 16 154 21 0.0 6 21 155 13 0.0 6 13 156 9 0.0 6 9 157 19 0.0 6 19 158 16 0.0 6 16 159 24 0.0 6 24 160 7 0.0 6 7 161 5 0.0 6 5 162 12 0.0 6 12 163 8 0.0 6 8 164 17 0.0 6 17 165 16 0.0 6 16 166 19 0.0 6 19 167 11 0.0 6 11 168 10 0.0 6 10 169 7 0.0 6 7 170 8 0.0 6 8 171 14 0.0 6 14 172 11 0.0 6 11 173 10 0.0 6 9 1 174 15 0.0 6 15 175 8 0.0 6 8 176 10 0.0 6 10 177 7 0.0 6 7 178 6 0.0 6 6 179 3 0.0 6 3 180 11 0.0 6 11 181 8 0.0 6 8 182 13 0.0 6 13 183 7 0.0 6 7 184 10 0.0 6 10 185 10 0.0 6 10 186 12 0.0 6 12 187 4 0.0 6 4 188 10 0.0 6 10 189 16 0.0 6 16 190 25 0.0 6 25 191 11 0.0 6 11 192 14 0.0 6 14 193 8 0.0 6 8 194 4 0.0 6 4 195 11 0.0 6 11 196 5 0.0 6 5 197 6 0.0 6 6 198 10 0.0 6 10 199 10 0.0 6 10 200 7 0.0 6 7 201 5 0.0 6 5 202 11 0.0 6 11 203 10 0.0 6 10 204 2 0.0 6 2 205 7 0.0 6 7 206 3 0.0 6 3 207 4 0.0 6 4 208 3 0.0 6 3 209 17 0.0 6 17 210 9 0.0 6 9 211 3 0.0 6 3 212 6 0.0 6 6 213 11 0.0 6 11 214 3 0.0 6 3 215 3 0.0 6 3 216 5 0.0 6 5 217 7 0.0 6 7 219 2 0.0 6 2 220 3 0.0 6 3 221 2 0.0 6 2 222 13 0.0 6 13 223 4 0.0 6 4 224 5 0.0 6 5 225 7 0.0 6 7 226 7 0.0 6 7 227 4 0.0 6 4 228 5 0.0 6 5 229 4 0.0 6 4 230 2 0.0 6 2 231 6 0.0 6 6 232 6 0.0 6 6 233 4 0.0 6 4 234 5 0.0 6 5 235 1 0.0 6 1 236 5 0.0 6 5 237 4 0.0 6 4 238 4 0.0 6 4 239 4 0.0 6 4 240 6 0.0 6 6 241 3 0.0 6 3 242 4 0.0 6 4 243 3 0.0 6 3 244 4 0.0 6 4 246 5 0.0 6 5 247 1 0.0 6 1 248 3 0.0 6 3 249 4 0.0 6 4 250 5 0.0 6 5 252 2 0.0 6 2 253 8 0.0 6 8 254 4 0.0 6 4 255 3 0.0 6 3 256 5 0.0 6 5 257 1 0.0 6 1 258 2 0.0 6 2 259 1 0.0 6 1 260 1 0.0 6 1 261 5 0.0 6 5 262 1 0.0 6 1 263 3 0.0 6 3 266 1 0.0 6 1 269 2 0.0 6 2 271 1 0.0 6 1 272 1 0.0 6 1 274 2 0.0 6 2 277 1 0.0 6 1 280 1 0.0 6 1 283 1 0.0 6 1 296 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 90684 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 91.4 1 1 7 6 5.7 2 5 1 12 8 0.0 3 8 13 8 0.0 3 7 0 1 14 11 0.0 4 10 1 15 6 0.0 4 5 1 16 11 0.0 4 9 2 17 29 0.0 5 25 2 2 18 8 0.0 5 7 1 19 76 0.0 5 66 10 20 115 0.0 6 110 5 21 58 0.0 6 58 22 48 0.0 6 45 3 23 31 0.0 6 29 2 24 28 0.0 6 27 0 1 25 27 0.0 6 26 1 26 15 0.0 6 15 27 24 0.0 6 23 0 0 0 1 28 26 0.0 6 20 5 0 1 29 22 0.0 6 21 1 30 19 0.0 6 19 31 26 0.0 6 24 1 0 0 0 0 1 32 28 0.0 6 25 3 33 18 0.0 6 18 34 17 0.0 6 16 1 35 14 0.0 6 14 36 11 0.0 6 9 1 0 1 37 26 0.0 6 23 1 1 1 38 15 0.0 6 14 1 39 19 0.0 6 17 2 40 13 0.0 6 13 41 7 0.0 6 3 3 0 0 0 0 1 42 20 0.0 6 20 43 26 0.0 6 23 2 0 1 44 24 0.0 6 20 3 0 0 0 1 45 25 0.0 6 24 1 46 15 0.0 6 14 0 1 47 19 0.0 6 17 1 0 0 0 1 48 26 0.0 6 23 3 49 20 0.0 6 18 2 50 19 0.0 6 15 3 0 1 51 30 0.0 6 28 2 52 19 0.0 6 16 2 1 53 24 0.0 6 22 1 0 0 0 1 54 29 0.0 6 25 1 3 55 32 0.0 6 32 56 26 0.0 6 25 0 1 57 31 0.0 6 28 3 58 22 0.0 6 21 1 59 30 0.0 6 26 2 2 60 22 0.0 6 18 1 1 0 1 1 61 54 0.0 6 53 1 62 21 0.0 6 21 63 39 0.0 6 38 0 0 0 1 64 35 0.0 6 32 3 65 48 0.0 6 45 2 0 0 0 1 66 36 0.0 6 34 2 67 47 0.0 6 44 3 68 36 0.0 6 31 3 0 2 69 68 0.0 6 63 4 0 1 70 191 0.0 6 183 7 0 0 1 71 65 0.0 6 60 3 0 0 1 0 1 72 90 0.0 6 88 2 73 49 0.0 6 47 2 74 65 0.0 6 61 4 75 46 0.0 6 45 1 76 38 0.0 6 38 77 62 0.0 6 59 1 1 1 78 66 0.0 6 64 2 79 58 0.0 6 57 1 80 67 0.0 6 65 2 81 53 0.0 6 49 4 82 85 0.0 6 80 5 83 69 0.0 6 67 1 0 0 0 0 1 84 78 0.0 6 76 2 85 95 0.0 6 89 5 0 1 86 101 0.0 6 97 4 87 66 0.0 6 66 88 64 0.0 6 62 2 89 63 0.0 6 61 1 0 0 0 1 90 75 0.0 6 73 2 91 67 0.0 6 63 3 0 1 92 86 0.0 6 82 2 1 0 0 0 1 93 93 0.0 6 90 3 94 88 0.0 6 83 5 95 77 0.0 6 75 2 96 125 0.0 6 119 3 1 0 0 1 1 97 108 0.0 6 105 2 0 0 0 0 1 98 107 0.0 6 101 1 4 1 99 144 0.0 6 142 1 0 0 0 0 1 100 96 0.0 6 93 2 0 0 1 101 92 0.0 6 92 102 92 0.0 6 91 0 1 103 118 0.0 6 110 7 0 0 1 104 122 0.0 6 121 1 105 120 0.0 6 116 2 0 1 0 0 1 106 143 0.0 6 140 2 0 1 107 248 0.0 6 236 9 0 1 2 108 148 0.0 6 145 3 109 142 0.0 6 136 5 1 110 158 0.0 6 156 1 1 111 137 0.0 6 133 3 0 0 1 112 153 0.0 6 150 3 113 176 0.0 6 170 6 114 175 0.0 6 168 6 0 0 0 0 1 115 226 0.0 6 225 0 0 0 0 0 1 116 198 0.0 6 192 5 0 0 0 1 117 207 0.0 6 204 3 118 218 0.0 6 213 5 119 209 0.0 6 204 2 0 1 1 1 120 238 0.0 6 237 1 121 294 0.0 6 287 7 122 221 0.0 6 217 3 0 0 1 123 222 0.0 6 216 5 0 0 1 124 235 0.0 6 232 1 0 0 1 0 1 125 249 0.0 6 245 2 1 0 1 126 314 0.0 6 310 3 0 1 127 370 0.0 6 365 4 0 0 1 128 414 0.0 6 410 4 129 448 0.0 6 442 5 1 130 445 0.0 6 438 6 0 0 1 131 491 0.0 6 480 9 0 0 1 0 1 132 593 0.0 6 584 8 0 0 0 1 133 539 0.0 6 533 4 0 1 0 0 1 134 581 0.0 6 575 5 1 135 562 0.0 6 552 6 2 0 1 0 1 136 595 0.0 6 591 4 137 668 0.0 6 658 8 1 0 1 138 692 0.0 6 680 9 1 0 1 0 1 139 702 0.0 6 690 12 140 774 0.0 6 769 5 141 888 0.0 6 883 3 0 0 0 1 1 142 1121 0.0 6 1110 10 1 143 1295 0.0 6 1282 11 0 0 2 144 1376 0.0 6 1360 15 1 145 1621 0.0 6 1610 7 0 1 2 0 1 146 1634 0.0 6 1626 6 2 147 1649 0.0 6 1631 13 0 1 1 1 2 148 2106 0.0 6 2087 18 0 0 0 1 149 2066 0.0 6 2050 14 1 0 1 150 1982 0.0 6 1962 19 0 1 151 2257 0.0 6 2244 9 1 0 1 0 2 152 1960 0.0 6 1940 17 1 0 0 0 2 153 2222 0.0 6 2202 14 2 0 1 3 154 1830 0.0 6 1817 10 1 0 1 0 1 155 2136 0.0 6 2119 15 0 0 0 0 2 156 2088 0.0 6 2072 16 157 1678 0.0 6 1658 18 1 1 158 1934 0.0 6 1921 12 0 0 0 1 159 1655 0.0 6 1644 11 160 1828 0.0 6 1820 6 0 1 1 161 1711 0.0 6 1700 10 0 0 0 1 162 1531 0.0 6 1524 7 163 1512 0.0 6 1506 4 0 0 2 164 1461 0.0 6 1446 14 0 0 1 165 1306 0.0 6 1293 10 0 1 0 1 1 166 1306 0.0 6 1294 11 0 0 0 1 167 1287 0.0 6 1281 6 168 1162 0.0 6 1140 21 0 0 1 169 1397 0.0 6 1387 3 0 1 4 0 2 170 1180 0.0 6 1175 4 0 0 0 0 1 171 1507 0.0 6 1500 6 0 0 0 0 1 172 1200 0.0 6 1195 5 173 1128 0.0 6 1123 5 174 1215 0.0 6 1208 7 175 1150 0.0 6 1141 6 0 0 1 0 2 176 1134 0.0 6 1125 6 0 0 1 1 1 177 1044 0.0 6 1036 6 1 0 1 178 1022 0.0 6 1014 7 0 0 0 0 1 179 1083 0.0 6 1077 5 0 0 1 180 881 0.0 6 873 5 1 1 0 1 181 800 0.0 6 792 7 0 0 1 182 679 0.0 6 675 3 0 0 0 1 183 612 0.0 6 606 6 184 579 0.0 6 574 3 1 0 0 0 1 185 555 0.0 6 551 4 186 867 0.0 6 856 9 0 1 0 1 187 950 0.0 6 943 5 1 1 188 830 0.0 6 826 2 1 0 0 1 189 554 0.0 6 548 6 190 545 0.0 6 542 2 0 1 191 543 0.0 6 540 2 0 1 192 498 0.0 6 497 1 193 441 0.0 6 436 4 0 0 0 0 1 194 450 0.0 6 443 6 0 0 1 195 357 0.0 6 352 5 196 289 0.0 6 286 3 197 278 0.0 6 276 2 198 275 0.0 6 270 5 199 325 0.0 6 323 2 200 257 0.0 6 255 2 201 209 0.0 6 208 0 1 202 221 0.0 6 219 2 203 193 0.0 6 189 3 0 0 0 1 204 159 0.0 6 159 205 172 0.0 6 172 206 135 0.0 6 134 1 207 131 0.0 6 128 3 208 116 0.0 6 115 0 1 209 108 0.0 6 108 210 116 0.0 6 116 211 69 0.0 6 68 1 212 85 0.0 6 84 1 213 55 0.0 6 55 214 62 0.0 6 61 1 215 69 0.0 6 67 1 1 216 79 0.0 6 78 1 217 71 0.0 6 70 1 218 60 0.0 6 60 219 54 0.0 6 54 220 37 0.0 6 35 2 221 34 0.0 6 34 222 28 0.0 6 28 223 22 0.0 6 22 224 37 0.0 6 36 1 225 30 0.0 6 30 226 23 0.0 6 23 227 15 0.0 6 15 228 14 0.0 6 13 1 229 12 0.0 6 12 230 15 0.0 6 15 231 11 0.0 6 11 232 8 0.0 6 7 1 233 5 0.0 6 5 234 3 0.0 6 2 1 235 6 0.0 6 4 2 236 5 0.0 6 5 237 2 0.0 6 2 238 7 0.0 6 7 239 5 0.0 6 5 240 1 0.0 6 1 241 4 0.0 6 4 242 3 0.0 6 3 243 4 0.0 6 3 0 0 0 1 244 6 0.0 6 6 245 3 0.0 6 3 246 1 0.0 6 1 247 1 0.0 6 1 248 2 0.0 6 2 249 1 0.0 6 1 250 1 0.0 6 1 251 2 0.0 6 2 253 1 0.0 6 1 254 1 0.0 6 1 256 1 0.0 6 1 278 1 0.0 6 1 279 1 0.0 6 0 0 0 0 0 1 280 1 0.0 6 1 281 7 0.0 6 7 282 51 0.0 6 49 0 0 0 0 1 1 283 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2817 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 2 0.0 6 0 2 49 1 0.0 6 1 60 3 0.0 6 3 61 24 0.0 6 24 64 3 0.0 6 1 2 65 46 0.0 6 45 1 67 2 0.0 6 1 1 68 27 0.0 6 26 1 69 14 0.0 6 14 70 32 0.0 6 32 71 24 0.0 6 24 72 29 0.0 6 29 73 26 0.0 6 26 74 33 0.0 6 33 75 26 0.0 6 24 2 76 71 0.0 6 71 77 58 0.0 6 58 78 52 0.0 6 50 2 79 65 0.0 6 65 80 30 0.0 6 29 0 0 0 1 81 23 0.0 6 22 0 0 0 1 82 63 0.0 6 62 0 0 1 83 29 0.0 6 29 84 26 0.0 6 26 85 20 0.0 6 20 86 26 0.0 6 26 87 26 0.0 6 25 0 0 0 1 88 30 0.0 6 30 89 41 0.0 6 41 90 40 0.0 6 40 91 33 0.0 6 32 1 92 27 0.0 6 26 1 93 33 0.0 6 33 94 22 0.0 6 22 95 70 0.0 6 68 1 0 0 1 96 18 0.0 6 18 97 24 0.0 6 24 98 36 0.0 6 36 99 34 0.0 6 33 0 0 0 1 100 25 0.0 6 25 101 31 0.0 6 31 102 77 0.0 6 77 103 18 0.0 6 17 1 104 21 0.0 6 21 105 26 0.0 6 25 0 0 1 106 21 0.0 6 19 1 0 0 1 107 32 0.0 6 32 108 34 0.0 6 34 109 6 0.0 6 5 1 110 37 0.0 6 35 2 111 17 0.0 6 16 0 0 0 1 112 28 0.0 6 28 113 30 0.0 6 30 114 22 0.0 6 21 0 0 1 115 11 0.0 6 10 1 116 14 0.0 6 14 117 17 0.0 6 17 118 18 0.0 6 18 119 9 0.0 6 9 120 17 0.0 6 16 1 121 13 0.0 6 13 122 21 0.0 6 21 123 30 0.0 6 30 124 10 0.0 6 10 125 26 0.0 6 26 126 20 0.0 6 20 127 13 0.0 6 13 128 19 0.0 6 19 129 17 0.0 6 17 130 39 0.0 6 39 131 47 0.0 6 47 132 30 0.0 6 30 133 18 0.0 6 18 134 17 0.0 6 17 135 18 0.0 6 17 0 0 1 136 28 0.0 6 28 137 15 0.0 6 15 138 25 0.0 6 22 0 0 0 0 3 139 19 0.0 6 19 140 16 0.0 6 16 141 21 0.0 6 21 142 21 0.0 6 21 143 9 0.0 6 9 144 24 0.0 6 24 145 12 0.0 6 12 146 19 0.0 6 19 147 14 0.0 6 14 148 15 0.0 6 15 149 23 0.0 6 23 150 20 0.0 6 20 151 12 0.0 6 11 0 0 0 0 0 1 152 10 0.0 6 10 153 14 0.0 6 13 1 154 17 0.0 6 17 155 18 0.0 6 18 156 12 0.0 6 10 1 0 0 1 157 15 0.0 6 14 0 0 0 1 158 19 0.0 6 19 159 11 0.0 6 11 160 7 0.0 6 7 161 7 0.0 6 7 162 25 0.0 6 24 0 0 0 1 163 20 0.0 6 20 164 10 0.0 6 10 165 7 0.0 6 7 166 11 0.0 6 11 167 17 0.0 6 17 168 9 0.0 6 8 1 169 15 0.0 6 15 170 5 0.0 6 5 171 13 0.0 6 13 172 15 0.0 6 15 173 7 0.0 6 7 174 13 0.0 6 13 175 8 0.0 6 8 176 8 0.0 6 8 177 5 0.0 6 5 178 9 0.0 6 9 179 3 0.0 6 3 180 4 0.0 6 4 181 9 0.0 6 9 182 9 0.0 6 9 183 3 0.0 6 3 184 3 0.0 6 2 0 0 0 1 185 9 0.0 6 9 186 8 0.0 6 8 187 8 0.0 6 8 188 6 0.0 6 6 189 5 0.0 6 5 190 5 0.0 6 5 191 3 0.0 6 3 192 8 0.0 6 8 193 5 0.0 6 5 194 8 0.0 6 8 195 5 0.0 6 5 196 3 0.0 6 3 197 7 0.0 6 7 198 5 0.0 6 4 1 200 5 0.0 6 5 201 1 0.0 6 1 202 4 0.0 6 4 204 6 0.0 6 6 205 3 0.0 6 3 206 1 0.0 6 1 208 2 0.0 6 1 0 0 0 1 209 3 0.0 6 3 211 2 0.0 6 2 214 1 0.0 6 1 215 1 0.0 6 1 218 1 0.0 6 1 219 1 0.0 6 1 220 2 0.0 6 2 221 2 0.0 6 2 222 1 0.0 6 1 223 1 0.0 6 1 224 1 0.0 6 1 225 1 0.0 6 1 229 1 0.0 6 1 232 1 0.0 6 1 237 1 0.0 6 1 250 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_18_129_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_18_356_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_18_129_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_18_356_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.29 s (59 µs/read; 1.01 M reads/minute). === Summary === Total read pairs processed: 89,368 Read 1 with adapter: 89,366 (100.0%) Read 2 with adapter: 89,316 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 54 (0.1%) Pairs written (passing filters): 89,314 (99.9%) Total basepairs processed: 45,781,429 bp Read 1: 25,335,960 bp Read 2: 20,445,469 bp Total written (filtered): 5,811,703 bp (12.7%) Read 1: 2,951,013 bp Read 2: 2,860,690 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 89275 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1396.4 0 0 1 6 2 21.8 1 0 0 2 7 1 5.5 2 0 0 1 9 1 0.3 2 0 0 1 15 1 0.0 4 1 16 2 0.0 4 2 17 1 0.0 5 1 19 1 0.0 5 1 20 18 0.0 6 18 21 9 0.0 6 8 1 22 2 0.0 6 2 23 9 0.0 6 8 1 24 3 0.0 6 2 1 25 8 0.0 6 8 26 12 0.0 6 11 0 1 27 5 0.0 6 5 28 3 0.0 6 3 29 5 0.0 6 5 30 3 0.0 6 3 31 9 0.0 6 9 32 2 0.0 6 2 33 2 0.0 6 2 34 4 0.0 6 4 35 3 0.0 6 3 36 3 0.0 6 2 1 37 2 0.0 6 2 38 8 0.0 6 7 1 39 1 0.0 6 1 40 7 0.0 6 6 1 41 1 0.0 6 1 43 2 0.0 6 2 44 7 0.0 6 7 45 17 0.0 6 16 1 46 17 0.0 6 17 47 5 0.0 6 5 48 2 0.0 6 2 49 1 0.0 6 1 50 4 0.0 6 4 51 2 0.0 6 2 52 4 0.0 6 4 53 7 0.0 6 6 1 54 4 0.0 6 3 1 55 6 0.0 6 5 1 56 13 0.0 6 13 57 5 0.0 6 5 58 6 0.0 6 4 2 59 5 0.0 6 5 60 2 0.0 6 1 1 61 1 0.0 6 1 62 6 0.0 6 6 63 7 0.0 6 7 64 8 0.0 6 7 1 65 7 0.0 6 7 66 1 0.0 6 1 67 4 0.0 6 4 68 3 0.0 6 3 69 3 0.0 6 3 70 1 0.0 6 1 71 1 0.0 6 1 72 2 0.0 6 1 1 73 6 0.0 6 5 1 74 5 0.0 6 5 75 3 0.0 6 3 77 1 0.0 6 1 78 7 0.0 6 7 79 1 0.0 6 1 80 4 0.0 6 3 1 81 1 0.0 6 1 82 1 0.0 6 1 83 2 0.0 6 2 84 2 0.0 6 2 86 4 0.0 6 4 87 7 0.0 6 7 88 2 0.0 6 1 1 89 8 0.0 6 8 90 4 0.0 6 4 91 3 0.0 6 3 92 5 0.0 6 4 1 93 4 0.0 6 4 94 2 0.0 6 1 1 95 1 0.0 6 1 96 1 0.0 6 1 97 5 0.0 6 5 98 1 0.0 6 1 99 9 0.0 6 7 2 100 2 0.0 6 1 1 101 15 0.0 6 14 1 102 10 0.0 6 7 3 103 6 0.0 6 6 104 4 0.0 6 4 105 24 0.0 6 24 106 8 0.0 6 5 2 1 107 8 0.0 6 8 108 20 0.0 6 19 1 109 2 0.0 6 2 110 2 0.0 6 2 111 9 0.0 6 9 112 10 0.0 6 10 113 16 0.0 6 16 114 6 0.0 6 6 115 18 0.0 6 15 3 116 4 0.0 6 4 117 9 0.0 6 9 118 5 0.0 6 5 119 11 0.0 6 10 1 120 3 0.0 6 3 121 28 0.0 6 26 2 122 36 0.0 6 36 123 18 0.0 6 16 2 124 15 0.0 6 15 125 10 0.0 6 9 1 126 38 0.0 6 37 0 1 127 61 0.0 6 60 1 128 67 0.0 6 64 3 129 34 0.0 6 34 130 17 0.0 6 16 1 131 13 0.0 6 13 132 8 0.0 6 7 1 133 11 0.0 6 10 1 134 27 0.0 6 26 1 135 14 0.0 6 13 1 136 17 0.0 6 16 1 137 44 0.0 6 44 138 27 0.0 6 25 2 139 30 0.0 6 30 140 18 0.0 6 18 141 44 0.0 6 43 1 142 23 0.0 6 23 143 102 0.0 6 102 144 29 0.0 6 29 145 97 0.0 6 97 146 69 0.0 6 69 147 41 0.0 6 40 1 148 32 0.0 6 32 149 52 0.0 6 49 3 150 71 0.0 6 70 1 151 30 0.0 6 30 152 28 0.0 6 27 1 153 29 0.0 6 29 154 48 0.0 6 47 1 155 51 0.0 6 50 1 156 145 0.0 6 144 1 157 33 0.0 6 33 158 27 0.0 6 26 1 159 53 0.0 6 53 160 61 0.0 6 59 2 161 229 0.0 6 228 1 162 42 0.0 6 42 163 42 0.0 6 41 1 164 86 0.0 6 86 165 70 0.0 6 70 166 49 0.0 6 48 1 167 150 0.0 6 147 3 168 70 0.0 6 70 169 54 0.0 6 53 1 170 196 0.0 6 194 2 171 170 0.0 6 169 1 172 35 0.0 6 35 173 74 0.0 6 74 174 56 0.0 6 54 2 175 29 0.0 6 27 2 176 69 0.0 6 67 2 177 62 0.0 6 56 6 178 107 0.0 6 101 6 179 83 0.0 6 78 5 180 56 0.0 6 55 1 181 66 0.0 6 65 1 182 46 0.0 6 45 1 183 29 0.0 6 29 184 27 0.0 6 26 0 0 1 185 39 0.0 6 37 2 186 25 0.0 6 23 2 187 54 0.0 6 53 1 188 58 0.0 6 55 3 189 75 0.0 6 73 2 190 36 0.0 6 36 191 70 0.0 6 67 3 192 77 0.0 6 73 4 193 246 0.0 6 241 5 194 149 0.0 6 142 7 195 273 0.0 6 269 4 196 210 0.0 6 204 6 197 271 0.0 6 265 6 198 524 0.0 6 511 10 3 199 144 0.0 6 133 11 200 426 0.0 6 416 10 201 131 0.0 6 126 5 202 180 0.0 6 174 5 1 203 216 0.0 6 209 6 0 1 204 261 0.0 6 257 4 205 256 0.0 6 247 9 206 330 0.0 6 319 11 207 224 0.0 6 214 10 208 424 0.0 6 409 14 1 209 262 0.0 6 253 9 210 467 0.0 6 458 9 211 387 0.0 6 376 10 0 1 212 361 0.0 6 347 13 0 1 213 741 0.0 6 719 22 214 709 0.0 6 685 23 1 215 398 0.0 6 387 11 216 420 0.0 6 411 9 217 180 0.0 6 175 5 218 169 0.0 6 168 1 219 151 0.0 6 145 6 220 184 0.0 6 182 2 221 195 0.0 6 188 7 222 178 0.0 6 172 3 3 223 168 0.0 6 159 9 224 658 0.0 6 644 14 225 149 0.0 6 142 7 226 339 0.0 6 326 12 1 227 220 0.0 6 214 6 228 131 0.0 6 127 3 0 1 229 171 0.0 6 166 5 230 286 0.0 6 274 12 231 890 0.0 6 878 12 232 423 0.0 6 414 9 233 338 0.0 6 322 16 234 615 0.0 6 603 12 235 421 0.0 6 401 20 236 1635 0.0 6 1612 22 1 237 424 0.0 6 415 9 238 306 0.0 6 292 13 1 239 561 0.0 6 541 18 1 1 240 609 0.0 6 585 23 1 241 1034 0.0 6 1000 32 1 0 1 242 1647 0.0 6 1590 55 2 243 2350 0.0 6 2287 63 244 1091 0.0 6 1060 30 1 245 1774 0.0 6 1740 31 3 246 1232 0.0 6 1179 52 1 247 3149 0.0 6 3078 70 1 248 796 0.0 6 778 18 249 675 0.0 6 650 25 250 1279 0.0 6 1237 42 251 1372 0.0 6 1343 29 252 647 0.0 6 615 31 0 0 1 253 1258 0.0 6 1231 26 1 254 847 0.0 6 817 30 255 729 0.0 6 713 14 1 1 256 359 0.0 6 352 6 1 257 378 0.0 6 361 16 1 258 403 0.0 6 394 9 259 438 0.0 6 417 19 1 1 260 858 0.0 6 831 26 1 261 653 0.0 6 629 23 1 262 1169 0.0 6 1130 37 2 263 1174 0.0 6 1139 35 264 996 0.0 6 957 38 1 265 1359 0.0 6 1318 41 266 820 0.0 6 797 22 1 267 566 0.0 6 547 18 0 0 0 1 268 508 0.0 6 482 25 1 269 732 0.0 6 716 14 2 270 752 0.0 6 738 14 271 1030 0.0 6 1002 27 1 272 1379 0.0 6 1334 41 4 273 1958 0.0 6 1882 71 4 1 274 5884 0.0 6 5741 140 3 275 5645 0.0 6 5540 100 3 0 2 276 2958 0.0 6 2838 118 1 1 277 16414 0.0 6 16026 376 10 1 0 1 278 1015 0.0 6 977 37 0 0 0 0 1 279 39 0.0 6 30 8 1 280 4 0.0 6 1 1 1 0 1 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 91 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 2 0.0 6 2 73 6 0.0 6 6 74 1 0.0 6 1 75 2 0.0 6 2 76 1 0.0 6 1 77 3 0.0 6 3 79 4 0.0 6 4 85 1 0.0 6 1 86 5 0.0 6 5 89 1 0.0 6 1 90 1 0.0 6 1 91 5 0.0 6 5 93 1 0.0 6 1 94 4 0.0 6 4 95 1 0.0 6 1 96 1 0.0 6 1 97 3 0.0 6 3 99 4 0.0 6 4 100 5 0.0 6 5 101 1 0.0 6 1 102 1 0.0 6 1 103 2 0.0 6 2 105 2 0.0 6 2 109 1 0.0 6 1 110 1 0.0 6 1 120 3 0.0 6 3 121 1 0.0 6 1 127 4 0.0 6 4 128 4 0.0 6 4 129 1 0.0 6 1 131 2 0.0 6 2 132 1 0.0 6 1 133 1 0.0 6 1 140 2 0.0 6 2 141 1 0.0 6 1 156 1 0.0 6 1 167 1 0.0 6 1 168 2 0.0 6 2 173 1 0.0 6 1 174 2 0.0 6 2 175 1 0.0 6 1 222 1 0.0 6 1 251 1 0.0 6 1 253 1 0.0 6 1 254 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 89238 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 349.1 1 1 10 6 0.1 3 6 11 5 0.0 3 5 12 1 0.0 3 0 1 13 5 0.0 3 4 1 14 4 0.0 4 4 15 2 0.0 4 1 1 16 8 0.0 4 8 17 13 0.0 5 11 2 18 11 0.0 5 10 1 19 8 0.0 5 7 1 20 23 0.0 6 22 1 21 22 0.0 6 22 22 23 0.0 6 23 23 18 0.0 6 17 1 24 15 0.0 6 13 2 25 13 0.0 6 12 1 26 12 0.0 6 12 27 9 0.0 6 8 1 28 10 0.0 6 10 29 14 0.0 6 13 1 30 15 0.0 6 12 3 31 8 0.0 6 8 32 7 0.0 6 7 33 5 0.0 6 4 0 1 34 10 0.0 6 10 35 3 0.0 6 3 36 3 0.0 6 3 37 11 0.0 6 9 1 0 1 38 12 0.0 6 12 39 7 0.0 6 4 2 0 0 1 40 10 0.0 6 10 41 8 0.0 6 7 1 42 4 0.0 6 4 43 18 0.0 6 16 2 44 14 0.0 6 12 2 45 8 0.0 6 6 1 1 46 15 0.0 6 14 1 47 14 0.0 6 12 2 48 8 0.0 6 7 0 1 49 10 0.0 6 8 2 50 16 0.0 6 15 1 51 11 0.0 6 10 0 0 0 0 0 1 52 23 0.0 6 22 1 53 16 0.0 6 13 2 0 0 0 1 54 22 0.0 6 21 1 55 13 0.0 6 12 1 56 11 0.0 6 9 0 1 0 1 57 15 0.0 6 9 5 1 58 16 0.0 6 12 2 0 2 59 5 0.0 6 5 60 14 0.0 6 13 1 61 11 0.0 6 11 62 7 0.0 6 7 63 7 0.0 6 5 2 64 12 0.0 6 9 3 65 18 0.0 6 15 3 66 23 0.0 6 20 0 1 1 1 67 154 0.0 6 145 6 1 0 1 0 1 68 35 0.0 6 33 2 69 56 0.0 6 55 1 70 9 0.0 6 8 1 71 31 0.0 6 31 72 29 0.0 6 28 1 73 9 0.0 6 8 1 74 10 0.0 6 9 1 75 15 0.0 6 13 2 76 20 0.0 6 20 77 14 0.0 6 13 0 1 78 40 0.0 6 40 79 26 0.0 6 26 80 18 0.0 6 14 3 1 81 12 0.0 6 11 1 82 44 0.0 6 43 1 83 68 0.0 6 59 8 0 0 1 84 49 0.0 6 48 1 85 19 0.0 6 19 86 15 0.0 6 14 1 87 37 0.0 6 33 3 1 88 22 0.0 6 16 5 1 89 30 0.0 6 27 3 90 31 0.0 6 29 1 1 91 38 0.0 6 35 3 92 23 0.0 6 18 5 93 41 0.0 6 36 3 1 0 1 94 56 0.0 6 53 3 95 23 0.0 6 22 1 96 39 0.0 6 36 1 0 0 2 97 34 0.0 6 33 1 98 36 0.0 6 34 2 99 30 0.0 6 25 5 100 31 0.0 6 29 2 101 32 0.0 6 30 2 102 55 0.0 6 51 3 1 103 69 0.0 6 65 4 104 54 0.0 6 53 1 105 51 0.0 6 50 1 106 36 0.0 6 33 2 0 1 107 32 0.0 6 27 4 1 108 45 0.0 6 44 1 109 44 0.0 6 39 5 110 44 0.0 6 43 1 111 44 0.0 6 40 4 112 33 0.0 6 32 1 113 42 0.0 6 41 1 114 71 0.0 6 62 5 4 115 55 0.0 6 47 7 1 116 36 0.0 6 33 3 117 31 0.0 6 30 1 118 45 0.0 6 42 3 119 26 0.0 6 25 1 120 58 0.0 6 52 6 121 65 0.0 6 59 6 122 71 0.0 6 65 6 123 49 0.0 6 46 3 124 84 0.0 6 81 3 125 63 0.0 6 58 4 0 0 1 126 46 0.0 6 45 1 127 58 0.0 6 54 3 1 128 32 0.0 6 28 2 2 129 38 0.0 6 34 4 130 34 0.0 6 33 1 131 41 0.0 6 41 132 171 0.0 6 163 8 133 161 0.0 6 155 6 134 175 0.0 6 170 4 1 135 231 0.0 6 221 10 136 267 0.0 6 256 10 0 0 1 137 144 0.0 6 136 8 138 288 0.0 6 277 11 139 237 0.0 6 232 4 1 140 125 0.0 6 121 3 0 0 1 141 334 0.0 6 321 12 1 142 263 0.0 6 249 13 0 0 1 143 423 0.0 6 412 9 2 144 197 0.0 6 189 7 1 145 194 0.0 6 184 9 0 1 146 92 0.0 6 88 4 147 195 0.0 6 186 6 1 0 1 1 148 340 0.0 6 321 18 1 149 484 0.0 6 468 13 2 0 1 150 286 0.0 6 274 11 1 151 518 0.0 6 499 17 2 152 375 0.0 6 364 10 1 153 650 0.0 6 625 22 2 1 154 397 0.0 6 387 9 0 0 1 155 332 0.0 6 324 7 0 0 1 156 410 0.0 6 397 11 0 0 1 1 157 480 0.0 6 464 16 158 314 0.0 6 307 7 159 442 0.0 6 431 10 0 1 160 278 0.0 6 268 8 2 161 203 0.0 6 195 5 1 1 1 162 412 0.0 6 397 14 0 0 1 163 474 0.0 6 453 19 1 1 164 496 0.0 6 479 15 1 0 1 165 702 0.0 6 673 28 0 1 166 324 0.0 6 315 9 167 883 0.0 6 858 21 2 1 1 168 429 0.0 6 416 13 169 361 0.0 6 352 9 170 654 0.0 6 632 19 3 171 274 0.0 6 265 9 172 378 0.0 6 366 11 1 173 639 0.0 6 619 18 1 0 1 174 1199 0.0 6 1159 36 2 0 2 175 810 0.0 6 767 40 2 0 1 176 1201 0.0 6 1148 49 2 1 1 177 1729 0.0 6 1675 49 3 0 2 178 691 0.0 6 660 28 1 0 2 179 665 0.0 6 638 25 1 1 180 1281 0.0 6 1258 23 181 205 0.0 6 195 10 182 332 0.0 6 322 7 3 183 394 0.0 6 376 16 2 184 594 0.0 6 560 33 1 185 1269 0.0 6 1221 45 2 0 1 186 1150 0.0 6 1121 29 187 726 0.0 6 693 29 2 1 1 188 955 0.0 6 921 33 0 0 1 189 736 0.0 6 717 19 190 733 0.0 6 708 23 1 0 1 191 952 0.0 6 916 35 1 192 1409 0.0 6 1361 48 193 450 0.0 6 439 10 1 194 814 0.0 6 786 27 1 195 1321 0.0 6 1288 32 1 196 821 0.0 6 786 33 2 197 434 0.0 6 415 19 198 797 0.0 6 753 42 0 1 1 199 736 0.0 6 709 24 1 1 1 200 1082 0.0 6 1052 27 1 0 2 201 550 0.0 6 527 21 1 0 1 202 658 0.0 6 630 25 3 203 289 0.0 6 279 10 204 381 0.0 6 370 11 205 461 0.0 6 434 26 1 206 542 0.0 6 523 19 207 435 0.0 6 416 15 3 1 208 365 0.0 6 355 9 0 0 0 1 209 490 0.0 6 468 18 3 0 1 210 795 0.0 6 749 44 2 211 3870 0.0 6 3764 93 6 3 2 2 212 653 0.0 6 616 34 2 0 1 213 1704 0.0 6 1616 81 4 2 1 214 5674 0.0 6 5506 159 6 0 3 215 1505 0.0 6 1439 63 1 0 2 216 3114 0.0 6 3022 84 6 1 1 217 886 0.0 6 856 29 0 0 1 218 980 0.0 6 944 31 2 0 3 219 856 0.0 6 826 23 5 1 1 220 571 0.0 6 545 23 2 0 1 221 1554 0.0 6 1509 44 1 222 1553 0.0 6 1502 49 2 223 522 0.0 6 507 15 224 931 0.0 6 910 19 2 225 994 0.0 6 960 31 1 0 2 226 906 0.0 6 872 31 1 0 2 227 538 0.0 6 522 14 2 228 1170 0.0 6 1127 42 1 229 1728 0.0 6 1666 60 1 1 230 386 0.0 6 372 12 0 1 1 231 342 0.0 6 333 9 232 308 0.0 6 286 21 1 233 545 0.0 6 522 21 2 234 1091 0.0 6 1057 33 1 235 1160 0.0 6 1124 35 0 0 1 236 565 0.0 6 547 17 1 237 185 0.0 6 175 9 0 0 1 238 796 0.0 6 759 36 0 0 1 239 1060 0.0 6 1029 31 240 820 0.0 6 790 29 1 241 1982 0.0 6 1929 49 2 0 2 242 674 0.0 6 659 14 0 0 1 243 509 0.0 6 499 8 0 0 2 244 370 0.0 6 358 12 245 131 0.0 6 126 5 246 163 0.0 6 157 6 247 170 0.0 6 167 3 248 540 0.0 6 519 20 0 1 249 164 0.0 6 154 10 250 320 0.0 6 309 11 251 284 0.0 6 269 14 0 1 252 70 0.0 6 67 3 253 42 0.0 6 42 254 26 0.0 6 24 2 255 7 0.0 6 5 2 256 3 0.0 6 3 257 4 0.0 6 4 258 3 0.0 6 3 259 3 0.0 6 3 260 2 0.0 6 2 261 1 0.0 6 1 262 1 0.0 6 1 263 2 0.0 6 2 264 4 0.0 6 4 265 2 0.0 6 2 266 3 0.0 6 3 267 3 0.0 6 3 275 1 0.0 6 0 0 0 1 277 1 0.0 6 1 278 4 0.0 6 4 279 17 0.0 6 17 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 78 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 1 0.0 6 1 72 2 0.0 6 2 73 1 0.0 6 1 74 1 0.0 6 1 75 1 0.0 6 1 76 1 0.0 6 0 1 78 1 0.0 6 1 81 1 0.0 6 1 82 2 0.0 6 2 83 1 0.0 6 1 84 1 0.0 6 1 85 4 0.0 6 4 90 12 0.0 6 12 92 1 0.0 6 1 93 2 0.0 6 2 96 6 0.0 6 6 98 2 0.0 6 2 99 8 0.0 6 8 100 4 0.0 6 4 101 1 0.0 6 1 102 2 0.0 6 1 0 0 1 109 1 0.0 6 1 110 1 0.0 6 1 127 4 0.0 6 4 130 1 0.0 6 1 131 5 0.0 6 5 135 1 0.0 6 1 136 1 0.0 6 1 139 1 0.0 6 1 140 2 0.0 6 2 147 2 0.0 6 2 156 1 0.0 6 1 168 1 0.0 6 1 173 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_19_130_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_19_357_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_19_130_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_19_357_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.88 s (70 µs/read; 0.86 M reads/minute). === Summary === Total read pairs processed: 55,552 Read 1 with adapter: 55,548 (100.0%) Read 2 with adapter: 55,519 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 36 (0.1%) Pairs written (passing filters): 55,516 (99.9%) Total basepairs processed: 27,334,494 bp Read 1: 15,602,462 bp Read 2: 11,732,032 bp Total written (filtered): 3,526,840 bp (12.9%) Read 1: 1,846,953 bp Read 2: 1,679,887 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 55284 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 868.0 0 1 2 4 1 217.0 1 1 6 4 13.6 1 1 1 2 7 1 3.4 2 0 0 1 13 1 0.0 3 1 15 1 0.0 4 1 16 1 0.0 4 1 19 2 0.0 5 2 20 20 0.0 6 20 21 9 0.0 6 9 22 6 0.0 6 6 23 12 0.0 6 12 24 7 0.0 6 7 25 13 0.0 6 13 26 8 0.0 6 8 27 5 0.0 6 5 28 4 0.0 6 4 29 3 0.0 6 3 31 4 0.0 6 4 34 4 0.0 6 4 35 1 0.0 6 0 1 37 4 0.0 6 2 2 38 6 0.0 6 6 39 1 0.0 6 1 40 2 0.0 6 2 41 3 0.0 6 2 0 1 44 7 0.0 6 6 1 45 4 0.0 6 4 46 7 0.0 6 7 47 3 0.0 6 3 48 2 0.0 6 2 49 3 0.0 6 3 50 4 0.0 6 4 51 1 0.0 6 1 52 3 0.0 6 3 54 1 0.0 6 1 55 1 0.0 6 1 56 1 0.0 6 0 0 1 57 3 0.0 6 3 58 1 0.0 6 1 60 1 0.0 6 1 61 3 0.0 6 3 62 2 0.0 6 2 63 2 0.0 6 2 64 3 0.0 6 3 65 1 0.0 6 1 66 2 0.0 6 2 67 6 0.0 6 6 68 1 0.0 6 1 69 2 0.0 6 2 71 1 0.0 6 1 72 1 0.0 6 1 73 4 0.0 6 4 74 1 0.0 6 1 75 1 0.0 6 1 76 1 0.0 6 1 77 4 0.0 6 4 78 5 0.0 6 3 2 79 2 0.0 6 2 82 2 0.0 6 2 83 3 0.0 6 3 84 2 0.0 6 2 86 6 0.0 6 6 87 3 0.0 6 3 88 6 0.0 6 6 89 5 0.0 6 5 90 2 0.0 6 1 1 91 2 0.0 6 2 92 3 0.0 6 3 93 2 0.0 6 2 94 2 0.0 6 2 96 2 0.0 6 2 97 2 0.0 6 2 98 10 0.0 6 10 99 3 0.0 6 3 100 4 0.0 6 4 101 7 0.0 6 7 102 6 0.0 6 6 103 7 0.0 6 7 104 5 0.0 6 3 2 105 6 0.0 6 5 0 0 1 106 3 0.0 6 3 107 3 0.0 6 3 108 9 0.0 6 9 109 11 0.0 6 11 110 7 0.0 6 7 111 8 0.0 6 8 112 15 0.0 6 15 113 10 0.0 6 10 114 6 0.0 6 5 0 0 0 0 0 1 115 9 0.0 6 8 1 116 4 0.0 6 4 117 7 0.0 6 7 118 12 0.0 6 12 119 5 0.0 6 5 120 11 0.0 6 10 1 121 10 0.0 6 9 1 122 27 0.0 6 27 123 15 0.0 6 15 124 7 0.0 6 7 125 10 0.0 6 10 126 13 0.0 6 13 127 17 0.0 6 17 128 20 0.0 6 19 1 129 19 0.0 6 19 130 12 0.0 6 12 131 5 0.0 6 5 132 8 0.0 6 7 1 133 19 0.0 6 18 1 134 15 0.0 6 15 135 19 0.0 6 18 1 136 18 0.0 6 17 1 137 29 0.0 6 29 138 14 0.0 6 13 1 139 29 0.0 6 29 140 36 0.0 6 35 1 141 59 0.0 6 59 142 228 0.0 6 228 143 271 0.0 6 269 1 1 144 225 0.0 6 221 3 1 145 156 0.0 6 155 1 146 967 0.0 6 965 2 147 155 0.0 6 155 148 98 0.0 6 95 3 149 181 0.0 6 177 4 150 169 0.0 6 169 151 373 0.0 6 373 152 407 0.0 6 407 153 107 0.0 6 106 1 154 56 0.0 6 56 155 39 0.0 6 37 2 156 78 0.0 6 77 1 157 147 0.0 6 145 2 158 88 0.0 6 88 159 44 0.0 6 44 160 68 0.0 6 64 4 161 79 0.0 6 78 1 162 451 0.0 6 449 2 163 120 0.0 6 119 1 164 46 0.0 6 45 1 165 53 0.0 6 52 1 166 39 0.0 6 38 1 167 45 0.0 6 44 1 168 70 0.0 6 67 3 169 168 0.0 6 167 1 170 150 0.0 6 149 1 171 35 0.0 6 35 172 56 0.0 6 55 1 173 67 0.0 6 67 174 90 0.0 6 89 1 175 32 0.0 6 32 176 38 0.0 6 38 177 30 0.0 6 29 1 178 41 0.0 6 41 179 44 0.0 6 40 3 0 1 180 42 0.0 6 41 1 181 84 0.0 6 82 2 182 102 0.0 6 99 3 183 274 0.0 6 270 4 184 64 0.0 6 63 1 185 140 0.0 6 138 2 186 120 0.0 6 118 2 187 108 0.0 6 103 5 188 146 0.0 6 142 4 189 415 0.0 6 414 1 190 159 0.0 6 154 5 191 104 0.0 6 100 4 192 97 0.0 6 94 2 1 193 162 0.0 6 159 3 194 140 0.0 6 136 4 195 151 0.0 6 142 9 196 155 0.0 6 147 8 197 248 0.0 6 238 9 1 198 119 0.0 6 114 4 1 199 130 0.0 6 122 8 200 197 0.0 6 189 8 201 188 0.0 6 182 5 1 202 197 0.0 6 191 6 203 175 0.0 6 171 4 204 155 0.0 6 153 2 205 224 0.0 6 218 6 206 231 0.0 6 228 3 207 189 0.0 6 183 5 1 208 127 0.0 6 122 5 209 174 0.0 6 167 7 210 123 0.0 6 121 2 211 103 0.0 6 102 1 212 100 0.0 6 96 3 1 213 176 0.0 6 173 3 214 205 0.0 6 197 8 215 247 0.0 6 239 8 216 136 0.0 6 129 7 217 144 0.0 6 140 3 0 1 218 160 0.0 6 156 4 219 118 0.0 6 115 2 0 1 220 105 0.0 6 104 1 221 99 0.0 6 91 8 222 199 0.0 6 193 6 223 185 0.0 6 177 8 224 255 0.0 6 251 4 225 188 0.0 6 183 4 1 226 209 0.0 6 205 4 227 107 0.0 6 103 3 1 228 129 0.0 6 126 3 229 134 0.0 6 131 3 230 126 0.0 6 123 3 231 128 0.0 6 126 2 232 114 0.0 6 108 6 233 71 0.0 6 70 1 234 98 0.0 6 98 235 94 0.0 6 92 2 236 154 0.0 6 149 4 0 1 237 325 0.0 6 311 13 1 238 280 0.0 6 271 9 239 333 0.0 6 322 11 240 215 0.0 6 207 8 241 333 0.0 6 323 10 242 231 0.0 6 228 3 243 201 0.0 6 192 9 244 123 0.0 6 114 9 245 218 0.0 6 208 10 246 206 0.0 6 195 10 1 247 186 0.0 6 175 11 248 189 0.0 6 188 1 249 188 0.0 6 183 5 250 242 0.0 6 237 5 251 165 0.0 6 154 11 252 195 0.0 6 186 9 253 133 0.0 6 128 4 1 254 144 0.0 6 138 6 255 200 0.0 6 191 9 256 359 0.0 6 349 10 257 192 0.0 6 190 2 258 230 0.0 6 218 10 2 259 346 0.0 6 333 11 2 260 181 0.0 6 173 8 261 218 0.0 6 214 4 262 99 0.0 6 97 2 263 102 0.0 6 97 5 264 109 0.0 6 106 3 265 235 0.0 6 217 18 266 101 0.0 6 93 8 267 172 0.0 6 166 6 268 551 0.0 6 522 29 269 238 0.0 6 231 7 270 319 0.0 6 311 8 271 144 0.0 6 136 8 272 307 0.0 6 286 19 1 0 0 0 1 273 768 0.0 6 739 28 1 274 535 0.0 6 514 21 275 519 0.0 6 505 12 1 1 276 543 0.0 6 523 19 1 277 3601 0.0 6 3434 150 15 0 2 278 25980 0.0 6 25384 582 13 0 1 279 270 0.0 6 250 17 3 280 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 264 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 73 1 0.0 6 1 74 8 0.0 6 8 75 10 0.0 6 10 76 11 0.0 6 11 77 16 0.0 6 16 78 30 0.0 6 30 79 2 0.0 6 2 80 3 0.0 6 3 81 5 0.0 6 4 1 82 6 0.0 6 6 83 16 0.0 6 16 84 12 0.0 6 12 85 2 0.0 6 2 86 1 0.0 6 1 87 2 0.0 6 2 88 3 0.0 6 3 89 2 0.0 6 2 90 3 0.0 6 3 91 2 0.0 6 2 92 2 0.0 6 2 93 5 0.0 6 5 94 17 0.0 6 16 1 95 2 0.0 6 2 96 2 0.0 6 2 97 3 0.0 6 3 98 1 0.0 6 1 99 2 0.0 6 2 100 2 0.0 6 2 101 7 0.0 6 7 102 5 0.0 6 5 103 1 0.0 6 1 105 1 0.0 6 1 106 3 0.0 6 3 115 7 0.0 6 7 116 2 0.0 6 2 120 3 0.0 6 3 121 13 0.0 6 13 122 1 0.0 6 1 125 1 0.0 6 1 128 6 0.0 6 6 129 3 0.0 6 3 136 1 0.0 6 1 141 1 0.0 6 0 1 154 1 0.0 6 1 156 1 0.0 6 1 163 1 0.0 6 1 164 1 0.0 6 1 168 1 0.0 6 1 172 1 0.0 6 1 173 5 0.0 6 5 174 7 0.0 6 7 178 1 0.0 6 1 185 2 0.0 6 2 190 2 0.0 6 2 191 6 0.0 6 6 201 1 0.0 6 1 202 2 0.0 6 2 206 1 0.0 6 1 221 1 0.0 6 1 222 1 0.0 6 1 255 1 0.0 6 1 272 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 55244 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 217.0 1 1 6 2 13.6 1 2 8 1 0.8 2 1 9 2 0.2 2 2 12 5 0.0 3 4 1 13 15 0.0 3 13 2 14 3 0.0 4 3 16 5 0.0 4 3 2 17 4 0.0 5 3 1 18 11 0.0 5 9 2 19 14 0.0 5 13 1 20 44 0.0 6 42 2 21 36 0.0 6 32 4 22 29 0.0 6 28 1 23 16 0.0 6 16 24 16 0.0 6 16 25 12 0.0 6 12 26 12 0.0 6 11 1 27 7 0.0 6 7 28 4 0.0 6 3 1 29 8 0.0 6 7 0 1 30 4 0.0 6 4 31 5 0.0 6 5 32 4 0.0 6 4 33 5 0.0 6 4 1 34 11 0.0 6 11 35 4 0.0 6 3 0 0 1 36 5 0.0 6 4 1 37 8 0.0 6 7 1 38 9 0.0 6 9 39 7 0.0 6 7 40 8 0.0 6 8 41 7 0.0 6 7 42 6 0.0 6 5 0 1 43 5 0.0 6 5 44 14 0.0 6 13 1 45 9 0.0 6 9 46 22 0.0 6 20 2 47 10 0.0 6 8 2 48 4 0.0 6 4 49 17 0.0 6 14 2 1 50 15 0.0 6 14 1 51 8 0.0 6 8 52 14 0.0 6 12 1 1 53 6 0.0 6 6 54 12 0.0 6 11 1 55 23 0.0 6 19 3 0 0 1 56 23 0.0 6 21 2 57 6 0.0 6 5 1 58 11 0.0 6 10 1 59 7 0.0 6 6 1 60 7 0.0 6 6 1 61 10 0.0 6 10 62 10 0.0 6 9 1 63 7 0.0 6 6 1 64 11 0.0 6 10 1 65 11 0.0 6 11 66 11 0.0 6 10 1 67 16 0.0 6 16 68 19 0.0 6 19 69 99 0.0 6 94 4 0 0 1 70 26 0.0 6 23 3 71 58 0.0 6 56 2 72 34 0.0 6 31 2 1 73 26 0.0 6 26 74 18 0.0 6 16 2 75 10 0.0 6 10 76 27 0.0 6 27 77 15 0.0 6 13 2 78 38 0.0 6 35 3 79 21 0.0 6 20 0 1 80 17 0.0 6 15 2 81 13 0.0 6 13 82 21 0.0 6 20 1 83 39 0.0 6 37 2 84 39 0.0 6 35 3 0 0 0 1 85 43 0.0 6 42 1 86 27 0.0 6 24 2 1 87 40 0.0 6 34 4 2 88 16 0.0 6 15 1 89 29 0.0 6 27 2 90 41 0.0 6 37 4 91 40 0.0 6 39 1 92 24 0.0 6 20 4 93 45 0.0 6 42 3 94 82 0.0 6 75 7 95 30 0.0 6 28 2 96 36 0.0 6 34 2 97 37 0.0 6 34 2 0 0 1 98 25 0.0 6 24 1 99 40 0.0 6 40 100 40 0.0 6 37 0 2 1 101 36 0.0 6 31 5 102 65 0.0 6 61 4 103 58 0.0 6 54 4 104 70 0.0 6 68 2 105 68 0.0 6 63 5 106 63 0.0 6 60 3 107 60 0.0 6 60 108 52 0.0 6 49 3 109 35 0.0 6 35 110 51 0.0 6 47 3 1 111 57 0.0 6 55 2 112 46 0.0 6 42 4 113 51 0.0 6 48 3 114 58 0.0 6 53 3 1 1 115 67 0.0 6 67 116 55 0.0 6 50 5 117 59 0.0 6 56 3 118 58 0.0 6 54 4 119 100 0.0 6 97 3 120 84 0.0 6 80 4 121 69 0.0 6 69 122 73 0.0 6 70 3 123 65 0.0 6 63 2 124 87 0.0 6 85 1 1 125 55 0.0 6 54 1 126 45 0.0 6 44 1 127 79 0.0 6 79 128 66 0.0 6 65 1 129 81 0.0 6 78 1 1 0 0 1 130 70 0.0 6 66 4 131 95 0.0 6 91 3 0 0 1 132 110 0.0 6 104 6 133 84 0.0 6 78 6 134 124 0.0 6 117 7 135 250 0.0 6 244 6 136 136 0.0 6 134 1 0 1 137 185 0.0 6 176 8 1 138 109 0.0 6 103 6 139 165 0.0 6 163 2 140 235 0.0 6 230 5 141 228 0.0 6 223 4 1 142 472 0.0 6 461 11 143 430 0.0 6 420 10 144 551 0.0 6 537 13 1 145 362 0.0 6 357 5 146 844 0.0 6 837 7 147 221 0.0 6 216 4 1 148 225 0.0 6 219 6 149 394 0.0 6 381 8 3 0 1 0 1 150 493 0.0 6 486 5 1 0 1 151 464 0.0 6 458 6 152 829 0.0 6 814 15 153 311 0.0 6 298 13 154 360 0.0 6 346 12 2 155 362 0.0 6 354 8 156 282 0.0 6 273 9 157 439 0.0 6 430 8 1 158 435 0.0 6 415 20 159 285 0.0 6 275 10 160 451 0.0 6 433 16 0 0 1 0 1 161 302 0.0 6 291 11 162 434 0.0 6 423 9 0 1 0 0 1 163 368 0.0 6 364 4 164 290 0.0 6 276 13 0 0 1 165 411 0.0 6 397 13 1 166 408 0.0 6 398 9 1 167 222 0.0 6 215 7 168 506 0.0 6 490 15 1 169 247 0.0 6 237 9 1 170 294 0.0 6 285 8 0 0 1 171 378 0.0 6 366 11 1 172 274 0.0 6 264 9 0 0 1 173 407 0.0 6 392 14 1 174 449 0.0 6 429 17 2 0 0 1 175 949 0.0 6 915 31 1 0 2 176 545 0.0 6 531 13 0 1 177 471 0.0 6 454 17 178 381 0.0 6 365 15 0 0 0 1 179 480 0.0 6 462 16 0 0 2 180 474 0.0 6 452 20 1 1 181 548 0.0 6 528 20 182 220 0.0 6 209 11 183 607 0.0 6 591 15 0 0 1 184 131 0.0 6 128 2 1 185 266 0.0 6 255 10 0 0 1 186 1122 0.0 6 1091 26 3 0 2 187 1080 0.0 6 1035 41 3 1 188 1123 0.0 6 1083 38 2 189 842 0.0 6 815 26 1 190 1441 0.0 6 1391 48 1 0 1 191 945 0.0 6 912 32 0 0 1 192 686 0.0 6 667 19 193 471 0.0 6 445 23 2 0 1 194 818 0.0 6 784 32 2 195 1263 0.0 6 1219 42 1 0 1 196 1649 0.0 6 1601 46 0 0 2 197 546 0.0 6 507 36 1 1 1 198 2027 0.0 6 1955 69 3 199 2251 0.0 6 2181 66 2 0 2 200 1143 0.0 6 1108 33 1 0 1 201 975 0.0 6 944 30 0 1 202 613 0.0 6 581 31 0 0 1 203 639 0.0 6 608 30 0 0 1 204 734 0.0 6 701 29 1 1 2 205 251 0.0 6 240 11 206 260 0.0 6 251 8 1 207 200 0.0 6 188 11 1 208 343 0.0 6 328 13 1 1 209 269 0.0 6 256 12 0 0 1 210 296 0.0 6 280 15 1 211 338 0.0 6 325 12 1 212 249 0.0 6 238 10 0 0 1 213 137 0.0 6 128 8 0 0 1 214 277 0.0 6 270 6 1 215 433 0.0 6 419 14 216 679 0.0 6 656 22 1 217 539 0.0 6 523 16 218 452 0.0 6 436 16 219 427 0.0 6 409 17 0 0 1 220 383 0.0 6 367 14 0 0 2 221 372 0.0 6 363 9 222 147 0.0 6 137 9 0 0 1 223 359 0.0 6 341 16 1 0 1 224 535 0.0 6 505 29 0 1 225 503 0.0 6 483 19 0 0 1 226 553 0.0 6 532 20 1 227 316 0.0 6 306 9 1 228 293 0.0 6 280 12 1 229 302 0.0 6 296 6 230 280 0.0 6 272 8 231 225 0.0 6 219 6 232 242 0.0 6 232 8 0 0 2 233 321 0.0 6 313 8 234 190 0.0 6 184 6 235 64 0.0 6 62 2 236 60 0.0 6 59 1 237 35 0.0 6 33 2 238 31 0.0 6 30 1 239 22 0.0 6 21 1 240 99 0.0 6 94 5 241 112 0.0 6 107 4 1 242 123 0.0 6 119 3 0 0 1 243 183 0.0 6 172 11 244 80 0.0 6 76 3 1 245 56 0.0 6 53 3 246 42 0.0 6 42 247 38 0.0 6 36 2 248 28 0.0 6 27 1 249 48 0.0 6 48 250 16 0.0 6 15 1 251 11 0.0 6 11 252 8 0.0 6 8 253 13 0.0 6 13 254 3 0.0 6 3 255 4 0.0 6 4 256 1 0.0 6 1 257 1 0.0 6 1 278 1 0.0 6 1 279 2 0.0 6 2 280 4 0.0 6 4 281 10 0.0 6 10 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 275 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 52 1 0.0 6 1 70 1 0.0 6 1 71 13 0.0 6 13 72 8 0.0 6 8 73 4 0.0 6 4 74 7 0.0 6 7 75 46 0.0 6 46 76 3 0.0 6 3 77 5 0.0 6 5 78 10 0.0 6 10 79 3 0.0 6 3 80 13 0.0 6 13 81 14 0.0 6 14 85 5 0.0 6 5 86 6 0.0 6 6 87 2 0.0 6 2 89 4 0.0 6 3 0 0 0 1 90 3 0.0 6 3 91 28 0.0 6 28 92 2 0.0 6 1 1 94 1 0.0 6 1 95 1 0.0 6 1 97 2 0.0 6 2 98 5 0.0 6 5 99 4 0.0 6 4 103 2 0.0 6 2 105 1 0.0 6 1 110 2 0.0 6 2 111 1 0.0 6 1 112 9 0.0 6 9 113 1 0.0 6 1 114 1 0.0 6 1 115 1 0.0 6 1 117 2 0.0 6 2 118 19 0.0 6 19 119 1 0.0 6 1 121 1 0.0 6 0 0 0 1 122 1 0.0 6 1 125 1 0.0 6 1 126 3 0.0 6 3 129 1 0.0 6 1 130 2 0.0 6 2 132 3 0.0 6 3 135 1 0.0 6 1 145 1 0.0 6 1 146 1 0.0 6 1 153 1 0.0 6 1 160 1 0.0 6 1 161 5 0.0 6 5 163 1 0.0 6 1 164 1 0.0 6 1 165 2 0.0 6 2 169 1 0.0 6 1 171 9 0.0 6 9 172 2 0.0 6 2 174 2 0.0 6 2 187 2 0.0 6 2 188 1 0.0 6 1 199 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_20_131_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_20_358_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_20_131_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_20_358_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.87 s (87267 µs/read; 0.00 M reads/minute). === Summary === Total read pairs processed: 10 Read 1 with adapter: 10 (100.0%) Read 2 with adapter: 10 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 0 (0.0%) Pairs written (passing filters): 10 (100.0%) Total basepairs processed: 5,125 bp Read 1: 2,887 bp Read 2: 2,238 bp Total written (filtered): 650 bp (12.7%) Read 1: 330 bp Read 2: 320 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 10 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 185 1 0.0 6 1 217 1 0.0 6 1 248 1 0.0 6 1 250 1 0.0 6 1 272 1 0.0 6 1 275 1 0.0 6 1 277 2 0.0 6 2 278 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 0 times === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 10 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 97 1 0.0 6 1 134 1 0.0 6 1 142 1 0.0 6 1 196 1 0.0 6 1 199 1 0.0 6 1 214 1 0.0 6 1 227 1 0.0 6 1 229 2 0.0 6 2 251 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 0 times cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_01_120_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_01_347_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_01_120_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_01_347_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_02_121_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_02_348_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_02_121_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_02_348_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_03_122_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_03_349_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_03_122_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_03_349_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_04_123_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_04_350_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_04_123_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_04_350_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_05_124_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_05_351_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_05_124_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_05_351_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_06_125_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_06_352_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_06_125_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_06_352_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_15_126_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_15_353_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_15_126_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_15_353_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_16_127_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_16_354_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_16_127_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_16_354_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_17_128_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_17_355_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_17_128_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_17_355_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_18_129_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_18_356_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_18_129_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_18_356_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_19_130_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_19_357_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_19_130_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_19_357_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_20_131_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_11-Rev_20_358_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_20_131_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_11-Rev_20_358_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_01_132_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_01_359_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_01_132_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_01_359_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.64 s (69 µs/read; 0.86 M reads/minute). === Summary === Total read pairs processed: 66,815 Read 1 with adapter: 66,809 (100.0%) Read 2 with adapter: 66,752 (99.9%) == Read fate breakdown == Pairs that were too short: 27 (0.0%) Pairs discarded as untrimmed: 69 (0.1%) Pairs written (passing filters): 66,719 (99.9%) Total basepairs processed: 28,922,897 bp Read 1: 16,668,282 bp Read 2: 12,254,615 bp Total written (filtered): 4,077,733 bp (14.1%) Read 1: 2,056,233 bp Read 2: 2,021,500 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 65415 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 1044.0 0 2 1 4 3 261.0 1 0 3 7 3 4.1 2 1 0 2 8 1 1.0 2 1 9 4 0.3 2 3 0 0 1 10 4 0.1 3 4 11 1 0.0 3 1 15 2 0.0 4 2 18 2 0.0 5 1 1 19 2 0.0 5 0 2 20 104 0.0 6 101 3 21 57 0.0 6 53 4 22 48 0.0 6 46 2 23 35 0.0 6 35 24 21 0.0 6 20 1 25 18 0.0 6 18 26 15 0.0 6 14 0 1 27 12 0.0 6 12 28 8 0.0 6 8 29 11 0.0 6 11 30 5 0.0 6 5 31 5 0.0 6 4 1 32 6 0.0 6 6 33 6 0.0 6 6 34 6 0.0 6 6 35 7 0.0 6 6 0 0 1 36 10 0.0 6 10 37 7 0.0 6 7 38 5 0.0 6 4 1 39 6 0.0 6 6 40 8 0.0 6 8 41 10 0.0 6 9 1 42 2 0.0 6 2 43 2 0.0 6 2 44 7 0.0 6 7 45 6 0.0 6 6 46 6 0.0 6 6 47 5 0.0 6 4 1 48 6 0.0 6 6 49 7 0.0 6 7 50 5 0.0 6 5 51 5 0.0 6 5 52 5 0.0 6 5 53 10 0.0 6 9 1 54 9 0.0 6 8 0 0 0 0 1 55 7 0.0 6 7 56 3 0.0 6 2 1 57 6 0.0 6 5 1 58 9 0.0 6 9 59 2 0.0 6 2 60 2 0.0 6 2 61 8 0.0 6 8 62 4 0.0 6 4 63 3 0.0 6 3 64 5 0.0 6 5 65 5 0.0 6 4 0 0 0 1 66 6 0.0 6 6 67 8 0.0 6 8 68 4 0.0 6 3 1 69 4 0.0 6 3 1 70 4 0.0 6 4 71 7 0.0 6 6 0 0 1 72 7 0.0 6 7 73 9 0.0 6 9 74 11 0.0 6 11 75 11 0.0 6 10 1 76 6 0.0 6 6 77 12 0.0 6 12 78 5 0.0 6 5 79 6 0.0 6 4 1 0 0 0 1 80 7 0.0 6 6 1 81 9 0.0 6 9 82 9 0.0 6 7 0 1 0 1 83 9 0.0 6 9 84 14 0.0 6 13 0 0 1 85 15 0.0 6 14 1 86 20 0.0 6 19 1 87 11 0.0 6 8 2 0 0 0 0 1 88 11 0.0 6 9 2 89 15 0.0 6 15 90 10 0.0 6 8 2 91 13 0.0 6 11 2 92 7 0.0 6 7 93 7 0.0 6 7 94 11 0.0 6 10 1 95 9 0.0 6 7 2 96 5 0.0 6 5 97 7 0.0 6 5 1 0 0 0 1 98 11 0.0 6 11 99 5 0.0 6 5 100 11 0.0 6 11 101 8 0.0 6 8 102 12 0.0 6 12 103 13 0.0 6 13 104 12 0.0 6 11 1 105 10 0.0 6 10 106 9 0.0 6 9 107 23 0.0 6 23 108 6 0.0 6 6 109 12 0.0 6 12 110 19 0.0 6 19 111 13 0.0 6 13 112 15 0.0 6 13 2 113 13 0.0 6 12 1 114 18 0.0 6 16 2 115 17 0.0 6 16 1 116 19 0.0 6 19 117 29 0.0 6 28 0 1 118 18 0.0 6 18 119 14 0.0 6 13 1 120 19 0.0 6 19 121 19 0.0 6 19 122 17 0.0 6 17 123 21 0.0 6 19 2 124 18 0.0 6 17 1 125 22 0.0 6 22 126 17 0.0 6 17 127 13 0.0 6 13 128 26 0.0 6 25 1 129 20 0.0 6 20 130 33 0.0 6 33 131 33 0.0 6 32 1 132 30 0.0 6 29 1 133 26 0.0 6 25 1 134 28 0.0 6 26 1 0 0 0 1 135 42 0.0 6 42 136 28 0.0 6 27 1 137 41 0.0 6 41 138 32 0.0 6 32 139 31 0.0 6 30 1 140 31 0.0 6 31 141 110 0.0 6 110 142 175 0.0 6 173 2 143 239 0.0 6 235 4 144 154 0.0 6 153 1 145 192 0.0 6 192 146 218 0.0 6 216 2 147 190 0.0 6 190 148 196 0.0 6 194 2 149 217 0.0 6 216 1 150 172 0.0 6 170 1 1 151 195 0.0 6 195 152 175 0.0 6 175 153 139 0.0 6 137 2 154 140 0.0 6 140 155 152 0.0 6 150 1 0 0 1 156 153 0.0 6 151 2 157 143 0.0 6 142 1 158 172 0.0 6 170 1 0 0 1 159 146 0.0 6 145 1 160 156 0.0 6 155 0 0 0 0 0 1 161 166 0.0 6 164 2 162 161 0.0 6 160 1 163 178 0.0 6 177 1 164 213 0.0 6 210 3 165 184 0.0 6 180 4 166 191 0.0 6 190 1 167 208 0.0 6 207 0 0 1 168 171 0.0 6 169 0 0 0 2 169 168 0.0 6 168 170 196 0.0 6 195 1 171 211 0.0 6 208 3 172 168 0.0 6 164 4 173 228 0.0 6 225 3 174 206 0.0 6 203 2 0 0 0 1 175 191 0.0 6 189 2 176 223 0.0 6 220 2 0 0 0 0 1 177 246 0.0 6 242 4 178 288 0.0 6 283 5 179 449 0.0 6 447 1 1 180 265 0.0 6 263 2 181 237 0.0 6 235 2 182 235 0.0 6 233 2 183 238 0.0 6 233 2 0 1 0 0 2 184 262 0.0 6 260 2 185 267 0.0 6 266 1 186 336 0.0 6 333 3 187 321 0.0 6 312 8 1 188 270 0.0 6 267 2 0 0 0 1 189 270 0.0 6 260 7 1 0 0 1 1 190 266 0.0 6 265 1 191 267 0.0 6 263 2 1 1 192 334 0.0 6 330 3 0 0 1 193 373 0.0 6 365 5 1 0 0 1 1 194 413 0.0 6 408 5 195 403 0.0 6 392 8 0 2 1 196 480 0.0 6 477 3 197 615 0.0 6 604 10 0 0 0 1 198 696 0.0 6 684 11 1 199 647 0.0 6 640 5 0 0 0 1 1 200 689 0.0 6 678 11 201 581 0.0 6 573 6 0 0 0 2 202 553 0.0 6 552 0 0 0 0 1 203 647 0.0 6 637 9 0 0 0 1 204 540 0.0 6 532 6 0 0 0 1 1 205 537 0.0 6 534 3 206 566 0.0 6 563 3 207 662 0.0 6 648 12 0 1 0 1 208 816 0.0 6 800 15 0 1 209 676 0.0 6 666 8 0 0 0 0 2 210 782 0.0 6 774 6 1 1 211 524 0.0 6 517 6 0 0 0 0 1 212 604 0.0 6 597 6 0 1 213 763 0.0 6 752 9 0 0 1 0 1 214 879 0.0 6 871 5 1 2 215 828 0.0 6 818 8 1 1 216 965 0.0 6 954 8 1 1 0 1 217 1094 0.0 6 1086 8 218 876 0.0 6 869 6 0 1 219 872 0.0 6 859 10 0 2 0 1 220 900 0.0 6 893 7 221 773 0.0 6 763 8 1 0 0 0 1 222 658 0.0 6 647 10 0 0 0 0 1 223 702 0.0 6 694 8 224 651 0.0 6 643 3 2 0 2 1 225 692 0.0 6 685 5 0 0 0 0 2 226 746 0.0 6 739 6 0 0 0 1 227 844 0.0 6 833 11 228 849 0.0 6 841 6 1 1 229 655 0.0 6 650 3 2 230 753 0.0 6 743 8 1 0 0 1 231 691 0.0 6 688 1 2 232 741 0.0 6 733 6 0 1 0 0 1 233 677 0.0 6 668 7 2 234 695 0.0 6 686 8 0 1 235 740 0.0 6 737 3 236 730 0.0 6 720 9 0 0 1 237 679 0.0 6 674 4 1 238 642 0.0 6 638 2 0 1 0 0 1 239 643 0.0 6 637 5 0 0 0 0 1 240 614 0.0 6 608 5 0 0 1 241 615 0.0 6 602 13 242 617 0.0 6 609 8 243 658 0.0 6 652 6 244 593 0.0 6 587 6 245 521 0.0 6 517 4 246 534 0.0 6 529 5 247 481 0.0 6 477 3 0 0 0 0 1 248 447 0.0 6 446 1 249 434 0.0 6 427 7 250 441 0.0 6 438 3 251 455 0.0 6 452 3 252 459 0.0 6 457 1 1 253 441 0.0 6 441 254 395 0.0 6 395 255 411 0.0 6 409 2 256 384 0.0 6 382 2 257 368 0.0 6 363 4 1 258 353 0.0 6 350 3 259 354 0.0 6 351 3 260 351 0.0 6 348 3 261 426 0.0 6 422 3 0 0 0 1 262 357 0.0 6 357 263 276 0.0 6 274 1 0 0 0 1 264 294 0.0 6 293 1 265 286 0.0 6 286 266 304 0.0 6 303 1 267 260 0.0 6 258 2 268 254 0.0 6 253 1 269 230 0.0 6 228 2 270 278 0.0 6 277 1 271 291 0.0 6 290 0 1 272 259 0.0 6 258 1 273 233 0.0 6 233 274 212 0.0 6 210 2 275 288 0.0 6 285 3 276 299 0.0 6 298 0 0 0 0 0 1 277 385 0.0 6 381 3 1 278 470 0.0 6 466 4 279 552 0.0 6 551 1 280 1895 0.0 6 1889 5 0 0 0 1 281 2322 0.0 6 2305 15 2 282 16 0.0 6 16 283 4 0.0 6 3 1 284 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1394 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 5 0.0 6 4 1 70 4 0.0 6 4 71 2 0.0 6 2 72 11 0.0 6 11 73 3 0.0 6 3 74 6 0.0 6 6 75 9 0.0 6 9 76 2 0.0 6 2 77 3 0.0 6 3 78 2 0.0 6 2 79 7 0.0 6 7 80 5 0.0 6 5 81 9 0.0 6 9 82 3 0.0 6 3 83 4 0.0 6 4 84 8 0.0 6 8 85 5 0.0 6 4 0 0 0 0 0 1 86 5 0.0 6 5 87 6 0.0 6 6 88 2 0.0 6 2 89 1 0.0 6 1 90 8 0.0 6 8 91 7 0.0 6 7 92 4 0.0 6 4 93 6 0.0 6 6 94 6 0.0 6 6 95 1 0.0 6 1 96 2 0.0 6 2 97 5 0.0 6 5 98 6 0.0 6 6 99 3 0.0 6 3 100 6 0.0 6 6 101 3 0.0 6 3 102 4 0.0 6 4 103 5 0.0 6 5 104 5 0.0 6 5 105 6 0.0 6 6 106 5 0.0 6 5 107 11 0.0 6 11 108 10 0.0 6 10 109 4 0.0 6 4 110 4 0.0 6 4 111 4 0.0 6 4 112 4 0.0 6 4 113 1 0.0 6 1 114 4 0.0 6 4 115 7 0.0 6 7 116 3 0.0 6 3 117 4 0.0 6 4 118 3 0.0 6 3 119 5 0.0 6 5 120 2 0.0 6 2 121 7 0.0 6 7 122 2 0.0 6 2 123 12 0.0 6 12 124 5 0.0 6 5 125 4 0.0 6 4 126 6 0.0 6 6 127 8 0.0 6 8 128 2 0.0 6 2 129 5 0.0 6 5 130 4 0.0 6 4 131 5 0.0 6 5 132 8 0.0 6 8 133 8 0.0 6 8 134 5 0.0 6 5 135 4 0.0 6 4 136 3 0.0 6 3 137 4 0.0 6 4 138 7 0.0 6 7 139 8 0.0 6 8 140 4 0.0 6 4 141 7 0.0 6 7 142 4 0.0 6 4 143 5 0.0 6 5 144 7 0.0 6 6 1 145 3 0.0 6 3 146 7 0.0 6 7 147 8 0.0 6 8 148 6 0.0 6 6 149 9 0.0 6 9 150 12 0.0 6 12 151 1 0.0 6 1 152 10 0.0 6 10 153 2 0.0 6 2 154 8 0.0 6 8 155 11 0.0 6 11 156 4 0.0 6 4 157 6 0.0 6 6 158 8 0.0 6 8 159 5 0.0 6 5 160 6 0.0 6 6 161 11 0.0 6 11 162 8 0.0 6 8 163 4 0.0 6 4 164 4 0.0 6 4 165 5 0.0 6 5 166 7 0.0 6 7 167 3 0.0 6 3 168 9 0.0 6 9 169 8 0.0 6 8 170 8 0.0 6 8 171 13 0.0 6 13 172 7 0.0 6 7 173 4 0.0 6 4 174 7 0.0 6 7 175 8 0.0 6 8 176 9 0.0 6 9 177 10 0.0 6 10 178 16 0.0 6 16 179 3 0.0 6 3 180 5 0.0 6 5 181 7 0.0 6 7 182 6 0.0 6 6 183 7 0.0 6 7 184 9 0.0 6 9 185 8 0.0 6 8 186 11 0.0 6 11 187 6 0.0 6 6 188 6 0.0 6 6 189 8 0.0 6 8 190 10 0.0 6 10 191 8 0.0 6 8 192 15 0.0 6 15 193 10 0.0 6 10 194 6 0.0 6 6 195 7 0.0 6 7 196 8 0.0 6 8 197 7 0.0 6 7 198 11 0.0 6 11 199 14 0.0 6 14 200 14 0.0 6 14 201 14 0.0 6 14 202 10 0.0 6 10 203 7 0.0 6 7 204 15 0.0 6 14 1 205 5 0.0 6 5 206 10 0.0 6 10 207 9 0.0 6 9 208 8 0.0 6 8 209 8 0.0 6 8 210 10 0.0 6 10 211 11 0.0 6 11 212 11 0.0 6 11 213 13 0.0 6 13 214 7 0.0 6 7 215 7 0.0 6 7 216 16 0.0 6 16 217 10 0.0 6 10 218 6 0.0 6 6 219 8 0.0 6 8 220 7 0.0 6 7 221 9 0.0 6 9 222 13 0.0 6 12 1 223 8 0.0 6 8 224 9 0.0 6 9 225 13 0.0 6 13 226 14 0.0 6 14 227 16 0.0 6 16 228 9 0.0 6 9 229 9 0.0 6 9 230 9 0.0 6 9 231 10 0.0 6 10 232 10 0.0 6 10 233 8 0.0 6 8 234 11 0.0 6 11 235 5 0.0 6 5 236 5 0.0 6 5 237 2 0.0 6 2 238 6 0.0 6 6 239 6 0.0 6 6 240 8 0.0 6 8 241 4 0.0 6 4 242 6 0.0 6 6 243 6 0.0 6 6 244 5 0.0 6 5 245 10 0.0 6 10 246 6 0.0 6 6 247 9 0.0 6 9 248 14 0.0 6 14 249 5 0.0 6 5 250 6 0.0 6 6 251 6 0.0 6 6 252 7 0.0 6 6 1 253 3 0.0 6 3 254 6 0.0 6 6 255 6 0.0 6 6 256 8 0.0 6 8 257 5 0.0 6 5 258 7 0.0 6 7 259 7 0.0 6 7 260 6 0.0 6 6 262 2 0.0 6 2 263 2 0.0 6 2 264 4 0.0 6 4 265 4 0.0 6 4 266 4 0.0 6 4 267 4 0.0 6 4 268 4 0.0 6 4 270 7 0.0 6 7 271 1 0.0 6 1 272 2 0.0 6 2 273 2 0.0 6 2 274 2 0.0 6 2 275 1 0.0 6 1 276 1 0.0 6 1 277 1 0.0 6 1 278 5 0.0 6 5 280 1 0.0 6 1 281 5 0.0 6 5 282 2 0.0 6 2 283 2 0.0 6 2 284 1 0.0 6 1 286 2 0.0 6 2 288 1 0.0 6 0 1 290 1 0.0 6 1 291 1 0.0 6 1 293 2 0.0 6 1 1 296 1 0.0 6 1 297 1 0.0 6 1 298 1 0.0 6 1 301 1 0.0 6 1 302 1 0.0 6 1 303 2 0.0 6 2 305 1 0.0 6 1 306 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 65951 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1044.0 0 1 6 1 16.3 1 0 1 8 1 1.0 2 1 9 1 0.3 2 1 10 1 0.1 3 0 1 11 2 0.0 3 2 12 2 0.0 3 1 1 13 15 0.0 3 14 1 14 10 0.0 4 9 1 16 13 0.0 4 12 1 17 27 0.0 5 26 1 18 13 0.0 5 11 2 19 40 0.0 5 37 3 20 115 0.0 6 111 3 0 1 21 78 0.0 6 77 1 22 41 0.0 6 38 3 23 39 0.0 6 37 2 24 26 0.0 6 26 25 32 0.0 6 30 2 26 22 0.0 6 22 27 27 0.0 6 27 28 26 0.0 6 22 2 0 0 1 0 1 29 22 0.0 6 22 30 27 0.0 6 26 1 31 28 0.0 6 28 32 22 0.0 6 19 2 0 0 1 33 20 0.0 6 19 1 34 13 0.0 6 11 1 0 1 35 9 0.0 6 7 0 1 0 0 1 36 20 0.0 6 19 1 37 20 0.0 6 19 1 38 21 0.0 6 21 39 19 0.0 6 18 1 40 13 0.0 6 10 3 41 24 0.0 6 22 2 42 31 0.0 6 28 3 43 32 0.0 6 30 2 44 17 0.0 6 17 45 19 0.0 6 19 46 22 0.0 6 20 2 47 17 0.0 6 17 48 20 0.0 6 19 1 49 19 0.0 6 19 50 23 0.0 6 23 51 32 0.0 6 31 1 52 28 0.0 6 26 2 53 29 0.0 6 29 54 25 0.0 6 22 3 55 26 0.0 6 23 2 0 1 56 24 0.0 6 22 2 57 30 0.0 6 26 4 58 38 0.0 6 37 1 59 41 0.0 6 41 60 33 0.0 6 31 2 61 32 0.0 6 30 2 62 29 0.0 6 28 1 63 29 0.0 6 26 1 1 1 64 31 0.0 6 31 65 54 0.0 6 51 1 1 1 66 45 0.0 6 45 67 29 0.0 6 25 4 68 69 0.0 6 66 2 1 69 130 0.0 6 124 6 70 84 0.0 6 78 5 0 0 0 1 71 81 0.0 6 74 7 72 52 0.0 6 50 2 73 64 0.0 6 59 4 0 0 0 0 1 74 50 0.0 6 46 3 0 1 75 44 0.0 6 40 3 0 0 0 0 1 76 49 0.0 6 43 6 77 54 0.0 6 49 4 0 0 1 78 55 0.0 6 54 1 79 52 0.0 6 49 2 1 80 61 0.0 6 54 6 0 0 1 81 51 0.0 6 48 3 82 74 0.0 6 70 2 0 1 0 1 83 79 0.0 6 74 4 1 84 73 0.0 6 67 4 0 2 85 84 0.0 6 82 2 86 61 0.0 6 57 4 87 59 0.0 6 54 4 0 0 0 1 88 82 0.0 6 77 3 1 0 0 0 1 89 80 0.0 6 76 3 1 90 52 0.0 6 50 2 91 67 0.0 6 64 3 92 87 0.0 6 86 0 1 93 84 0.0 6 83 0 0 0 1 94 95 0.0 6 90 5 95 106 0.0 6 102 2 1 0 1 96 85 0.0 6 82 3 97 104 0.0 6 102 2 98 136 0.0 6 131 3 0 2 99 108 0.0 6 103 3 0 1 0 1 100 102 0.0 6 99 3 101 105 0.0 6 101 4 102 107 0.0 6 105 2 103 106 0.0 6 102 4 104 140 0.0 6 133 7 105 162 0.0 6 151 9 0 2 106 251 0.0 6 245 3 0 0 3 107 157 0.0 6 153 4 108 128 0.0 6 126 1 0 0 0 0 1 109 167 0.0 6 160 6 1 110 173 0.0 6 168 5 111 138 0.0 6 135 2 0 0 0 0 1 112 193 0.0 6 181 10 0 2 113 175 0.0 6 167 7 0 0 0 0 1 114 208 0.0 6 200 5 1 0 0 0 2 115 209 0.0 6 201 5 2 1 116 184 0.0 6 178 5 0 0 0 1 117 244 0.0 6 235 7 0 0 1 1 118 226 0.0 6 218 7 1 119 235 0.0 6 228 6 0 1 120 259 0.0 6 254 5 121 232 0.0 6 223 9 122 243 0.0 6 239 4 123 249 0.0 6 238 7 0 0 2 2 124 269 0.0 6 258 7 2 1 0 0 1 125 276 0.0 6 268 3 1 1 2 1 126 293 0.0 6 283 9 0 0 0 1 127 372 0.0 6 358 13 1 128 394 0.0 6 383 9 1 0 1 129 356 0.0 6 342 13 0 0 0 0 1 130 483 0.0 6 472 9 1 1 131 481 0.0 6 470 9 0 1 0 0 1 132 439 0.0 6 426 10 0 1 0 0 2 133 541 0.0 6 520 17 2 1 0 1 134 508 0.0 6 494 11 0 0 0 1 2 135 575 0.0 6 561 12 1 0 0 1 136 663 0.0 6 639 20 1 1 1 1 137 616 0.0 6 598 15 1 0 1 0 1 138 582 0.0 6 566 15 0 0 1 139 657 0.0 6 638 14 1 1 2 0 1 140 700 0.0 6 685 15 141 685 0.0 6 671 13 0 0 1 142 793 0.0 6 770 19 2 0 1 1 143 889 0.0 6 870 16 1 1 0 1 144 834 0.0 6 812 19 2 0 0 1 145 863 0.0 6 844 14 1 0 1 0 3 146 962 0.0 6 940 20 0 0 1 0 1 147 939 0.0 6 916 18 2 1 0 2 148 1031 0.0 6 1006 18 2 1 1 1 2 149 1014 0.0 6 993 15 0 2 2 1 1 150 1198 0.0 6 1169 23 3 0 2 1 151 1309 0.0 6 1265 37 2 3 2 152 1681 0.0 6 1643 28 5 2 1 2 153 1131 0.0 6 1107 19 1 1 2 0 1 154 1620 0.0 6 1586 29 1 2 1 1 155 1428 0.0 6 1395 29 1 0 2 0 1 156 1111 0.0 6 1088 19 1 0 1 0 2 157 1236 0.0 6 1204 27 2 1 1 1 158 1166 0.0 6 1140 20 2 1 1 2 159 1168 0.0 6 1141 24 0 1 2 160 1171 0.0 6 1137 28 3 0 1 2 161 950 0.0 6 924 19 1 2 2 1 1 162 1013 0.0 6 985 18 5 1 1 0 3 163 934 0.0 6 916 13 1 1 1 0 2 164 819 0.0 6 800 18 1 165 835 0.0 6 820 12 0 2 1 166 778 0.0 6 762 14 0 0 1 0 1 167 748 0.0 6 735 12 0 0 0 1 168 904 0.0 6 883 18 0 1 0 0 2 169 734 0.0 6 720 14 170 900 0.0 6 881 17 1 0 0 0 1 171 791 0.0 6 781 6 0 1 1 1 1 172 825 0.0 6 802 17 2 0 1 3 173 889 0.0 6 876 12 0 0 0 0 1 174 871 0.0 6 857 11 1 1 0 0 1 175 837 0.0 6 823 9 1 1 0 1 2 176 804 0.0 6 794 10 177 677 0.0 6 662 13 2 178 741 0.0 6 728 12 0 0 1 179 584 0.0 6 578 6 180 574 0.0 6 566 6 0 0 2 181 458 0.0 6 450 8 182 459 0.0 6 451 6 1 0 1 183 442 0.0 6 438 4 184 461 0.0 6 459 1 0 0 1 185 618 0.0 6 614 4 186 792 0.0 6 782 6 0 1 1 1 1 187 712 0.0 6 701 9 1 1 188 495 0.0 6 487 6 0 0 1 1 189 429 0.0 6 425 4 190 419 0.0 6 414 5 191 412 0.0 6 406 6 192 359 0.0 6 357 2 193 385 0.0 6 377 5 1 1 1 194 311 0.0 6 309 1 0 0 0 0 1 195 307 0.0 6 302 4 0 0 1 196 244 0.0 6 241 2 0 0 0 0 1 197 225 0.0 6 222 3 198 305 0.0 6 300 3 0 0 1 1 199 231 0.0 6 228 3 200 234 0.0 6 230 2 0 0 1 0 1 201 205 0.0 6 204 1 202 183 0.0 6 182 0 0 1 203 149 0.0 6 149 204 137 0.0 6 135 2 205 136 0.0 6 133 2 0 0 1 206 150 0.0 6 147 2 0 1 207 141 0.0 6 141 208 112 0.0 6 111 0 1 209 104 0.0 6 104 210 93 0.0 6 93 211 73 0.0 6 71 2 212 64 0.0 6 63 1 213 95 0.0 6 94 1 214 99 0.0 6 99 215 96 0.0 6 95 1 216 99 0.0 6 99 217 64 0.0 6 62 2 218 51 0.0 6 51 219 61 0.0 6 60 1 220 48 0.0 6 48 221 32 0.0 6 31 1 222 29 0.0 6 29 223 44 0.0 6 44 224 43 0.0 6 42 1 225 30 0.0 6 30 226 23 0.0 6 23 227 19 0.0 6 19 228 16 0.0 6 16 229 17 0.0 6 17 230 18 0.0 6 18 231 14 0.0 6 12 2 232 7 0.0 6 7 233 5 0.0 6 5 234 10 0.0 6 10 235 6 0.0 6 6 236 6 0.0 6 6 237 8 0.0 6 8 238 2 0.0 6 2 239 4 0.0 6 4 240 3 0.0 6 3 241 4 0.0 6 4 242 2 0.0 6 2 243 2 0.0 6 2 244 7 0.0 6 7 245 1 0.0 6 1 246 1 0.0 6 1 248 3 0.0 6 3 249 1 0.0 6 1 251 1 0.0 6 1 253 1 0.0 6 1 254 1 0.0 6 1 258 1 0.0 6 1 277 2 0.0 6 1 1 279 3 0.0 6 3 280 20 0.0 6 20 281 110 0.0 6 107 2 1 282 4 0.0 6 4 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 801 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 50 1 0.0 6 1 51 1 0.0 6 1 54 1 0.0 6 1 69 2 0.0 6 2 70 2 0.0 6 2 71 7 0.0 6 7 72 7 0.0 6 7 73 2 0.0 6 2 74 5 0.0 6 5 75 9 0.0 6 9 76 11 0.0 6 11 77 5 0.0 6 5 78 5 0.0 6 4 1 79 10 0.0 6 10 80 3 0.0 6 3 81 5 0.0 6 5 82 3 0.0 6 3 83 4 0.0 6 4 84 4 0.0 6 4 85 6 0.0 6 6 86 5 0.0 6 5 87 6 0.0 6 6 88 4 0.0 6 4 89 3 0.0 6 3 90 2 0.0 6 2 92 3 0.0 6 3 93 8 0.0 6 8 94 8 0.0 6 8 95 3 0.0 6 3 96 3 0.0 6 3 97 6 0.0 6 6 98 6 0.0 6 6 99 5 0.0 6 5 100 6 0.0 6 6 101 4 0.0 6 4 102 6 0.0 6 6 103 7 0.0 6 7 104 3 0.0 6 2 0 0 0 1 105 4 0.0 6 4 106 10 0.0 6 10 107 10 0.0 6 10 108 8 0.0 6 8 109 8 0.0 6 8 110 3 0.0 6 3 111 4 0.0 6 4 112 2 0.0 6 2 113 3 0.0 6 3 114 1 0.0 6 1 115 9 0.0 6 9 116 6 0.0 6 6 117 4 0.0 6 4 118 4 0.0 6 4 119 5 0.0 6 5 120 5 0.0 6 5 121 4 0.0 6 4 122 3 0.0 6 3 123 4 0.0 6 4 124 2 0.0 6 2 125 7 0.0 6 7 126 11 0.0 6 11 127 4 0.0 6 4 128 2 0.0 6 2 129 3 0.0 6 3 130 8 0.0 6 8 131 8 0.0 6 8 132 10 0.0 6 10 133 3 0.0 6 3 134 6 0.0 6 6 135 9 0.0 6 9 136 9 0.0 6 8 1 137 7 0.0 6 7 138 4 0.0 6 4 139 8 0.0 6 8 140 9 0.0 6 8 1 141 2 0.0 6 2 142 7 0.0 6 7 143 7 0.0 6 7 144 8 0.0 6 8 145 9 0.0 6 9 146 7 0.0 6 7 147 5 0.0 6 5 148 6 0.0 6 6 149 8 0.0 6 8 150 2 0.0 6 2 151 2 0.0 6 2 152 7 0.0 6 7 153 4 0.0 6 4 154 6 0.0 6 6 155 7 0.0 6 7 156 3 0.0 6 3 157 8 0.0 6 8 158 8 0.0 6 8 159 6 0.0 6 6 160 7 0.0 6 7 161 4 0.0 6 4 162 5 0.0 6 5 163 8 0.0 6 7 0 0 1 164 7 0.0 6 7 165 8 0.0 6 8 166 4 0.0 6 4 167 10 0.0 6 10 168 5 0.0 6 4 1 169 6 0.0 6 6 170 7 0.0 6 7 171 6 0.0 6 6 172 9 0.0 6 9 173 4 0.0 6 4 174 7 0.0 6 7 175 6 0.0 6 5 1 176 4 0.0 6 4 177 11 0.0 6 11 178 4 0.0 6 4 179 2 0.0 6 2 180 4 0.0 6 4 181 6 0.0 6 6 182 4 0.0 6 4 183 9 0.0 6 9 184 9 0.0 6 9 185 14 0.0 6 14 186 6 0.0 6 5 1 187 10 0.0 6 10 188 3 0.0 6 3 189 5 0.0 6 5 190 5 0.0 6 5 191 4 0.0 6 4 192 9 0.0 6 9 193 3 0.0 6 3 194 1 0.0 6 1 195 1 0.0 6 1 196 5 0.0 6 5 197 3 0.0 6 3 198 2 0.0 6 2 199 10 0.0 6 10 200 4 0.0 6 4 201 4 0.0 6 4 202 2 0.0 6 2 203 1 0.0 6 1 204 5 0.0 6 5 205 1 0.0 6 1 206 5 0.0 6 5 209 4 0.0 6 4 210 2 0.0 6 2 211 5 0.0 6 5 212 1 0.0 6 1 213 2 0.0 6 2 215 3 0.0 6 3 216 5 0.0 6 5 217 3 0.0 6 3 218 1 0.0 6 1 221 3 0.0 6 3 222 2 0.0 6 2 225 1 0.0 6 1 226 2 0.0 6 2 227 1 0.0 6 1 230 1 0.0 6 1 231 1 0.0 6 1 232 1 0.0 6 1 237 1 0.0 6 1 238 1 0.0 6 1 240 1 0.0 6 1 242 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_02_133_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_02_360_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_02_133_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_02_360_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.77 s (69 µs/read; 0.87 M reads/minute). === Summary === Total read pairs processed: 84,050 Read 1 with adapter: 84,036 (100.0%) Read 2 with adapter: 84,050 (100.0%) == Read fate breakdown == Pairs that were too short: 10 (0.0%) Pairs discarded as untrimmed: 14 (0.0%) Pairs written (passing filters): 84,026 (100.0%) Total basepairs processed: 39,601,213 bp Read 1: 22,774,165 bp Read 2: 16,827,048 bp Total written (filtered): 5,164,792 bp (13.0%) Read 1: 2,548,071 bp Read 2: 2,616,721 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 83417 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1313.3 0 1 6 2 20.5 1 0 0 2 7 1 5.1 2 1 8 2 1.3 2 1 0 0 1 9 1 0.3 2 1 10 3 0.1 3 3 18 1 0.0 5 0 1 19 2 0.0 5 2 20 59 0.0 6 58 1 21 35 0.0 6 35 22 32 0.0 6 32 23 23 0.0 6 22 1 24 14 0.0 6 14 25 19 0.0 6 19 26 6 0.0 6 6 27 4 0.0 6 4 28 5 0.0 6 5 29 11 0.0 6 11 30 4 0.0 6 4 31 2 0.0 6 2 32 4 0.0 6 4 33 2 0.0 6 2 34 7 0.0 6 7 35 3 0.0 6 3 36 2 0.0 6 1 1 37 4 0.0 6 2 2 38 2 0.0 6 2 39 8 0.0 6 8 40 1 0.0 6 1 41 1 0.0 6 1 42 3 0.0 6 3 43 7 0.0 6 7 44 6 0.0 6 6 45 5 0.0 6 5 46 8 0.0 6 8 47 3 0.0 6 3 48 4 0.0 6 4 49 2 0.0 6 1 1 50 4 0.0 6 4 51 2 0.0 6 2 52 4 0.0 6 4 53 9 0.0 6 8 1 54 5 0.0 6 5 55 3 0.0 6 3 56 8 0.0 6 8 57 3 0.0 6 3 58 6 0.0 6 6 59 4 0.0 6 4 60 3 0.0 6 3 61 4 0.0 6 4 62 4 0.0 6 2 1 1 63 3 0.0 6 3 64 5 0.0 6 5 65 5 0.0 6 5 66 4 0.0 6 4 67 4 0.0 6 4 68 11 0.0 6 9 2 69 7 0.0 6 7 70 2 0.0 6 2 71 4 0.0 6 2 0 0 0 1 1 72 3 0.0 6 3 73 2 0.0 6 2 74 4 0.0 6 4 75 12 0.0 6 12 76 6 0.0 6 5 1 77 3 0.0 6 3 78 9 0.0 6 7 1 1 79 1 0.0 6 1 80 6 0.0 6 5 1 81 3 0.0 6 3 82 4 0.0 6 4 83 2 0.0 6 2 84 6 0.0 6 6 85 12 0.0 6 12 86 16 0.0 6 15 1 87 9 0.0 6 9 88 8 0.0 6 8 89 6 0.0 6 6 90 5 0.0 6 5 91 3 0.0 6 2 1 92 5 0.0 6 5 93 4 0.0 6 4 94 7 0.0 6 7 95 3 0.0 6 3 96 5 0.0 6 4 1 97 6 0.0 6 6 98 6 0.0 6 6 99 7 0.0 6 7 100 8 0.0 6 7 1 101 7 0.0 6 7 102 7 0.0 6 7 103 5 0.0 6 5 104 10 0.0 6 9 1 105 9 0.0 6 9 106 16 0.0 6 16 107 17 0.0 6 17 108 13 0.0 6 11 1 1 109 12 0.0 6 11 1 110 13 0.0 6 13 111 11 0.0 6 11 112 13 0.0 6 12 1 113 14 0.0 6 13 1 114 15 0.0 6 15 115 12 0.0 6 9 3 116 5 0.0 6 5 117 18 0.0 6 16 1 0 1 118 16 0.0 6 16 119 14 0.0 6 12 2 120 8 0.0 6 7 0 1 121 21 0.0 6 18 3 122 23 0.0 6 22 1 123 19 0.0 6 18 1 124 24 0.0 6 24 125 20 0.0 6 18 2 126 16 0.0 6 16 127 18 0.0 6 17 1 128 21 0.0 6 21 129 16 0.0 6 14 2 130 18 0.0 6 18 131 18 0.0 6 18 132 18 0.0 6 17 1 133 19 0.0 6 18 1 134 20 0.0 6 20 135 26 0.0 6 26 136 24 0.0 6 24 137 43 0.0 6 42 1 138 49 0.0 6 47 2 139 41 0.0 6 39 2 140 33 0.0 6 31 2 141 60 0.0 6 58 0 2 142 99 0.0 6 97 2 143 194 0.0 6 194 144 262 0.0 6 262 145 155 0.0 6 153 2 146 140 0.0 6 138 2 147 183 0.0 6 183 148 168 0.0 6 166 2 149 166 0.0 6 164 2 150 194 0.0 6 188 5 1 151 175 0.0 6 175 152 158 0.0 6 157 1 153 155 0.0 6 155 154 140 0.0 6 139 1 155 103 0.0 6 102 1 156 125 0.0 6 125 157 139 0.0 6 138 1 158 123 0.0 6 121 2 159 148 0.0 6 146 2 160 162 0.0 6 157 5 161 165 0.0 6 162 3 162 195 0.0 6 193 1 1 163 161 0.0 6 159 2 164 146 0.0 6 145 1 165 160 0.0 6 158 1 0 1 166 163 0.0 6 162 1 167 137 0.0 6 135 1 1 168 111 0.0 6 111 169 133 0.0 6 130 3 170 153 0.0 6 150 3 171 179 0.0 6 177 1 1 172 160 0.0 6 158 2 173 153 0.0 6 152 1 174 199 0.0 6 195 4 175 198 0.0 6 192 5 1 176 160 0.0 6 158 2 177 225 0.0 6 219 6 178 201 0.0 6 199 2 179 199 0.0 6 195 4 180 375 0.0 6 372 2 1 181 233 0.0 6 230 3 182 202 0.0 6 198 4 183 216 0.0 6 213 2 1 184 290 0.0 6 286 4 185 276 0.0 6 265 11 186 351 0.0 6 342 9 187 227 0.0 6 224 3 188 230 0.0 6 224 6 189 247 0.0 6 243 4 190 207 0.0 6 203 3 0 1 191 225 0.0 6 223 2 192 251 0.0 6 246 5 193 293 0.0 6 286 6 0 0 1 194 314 0.0 6 308 6 195 340 0.0 6 337 3 196 410 0.0 6 406 4 197 405 0.0 6 396 8 1 198 590 0.0 6 582 8 199 582 0.0 6 570 11 0 0 0 0 1 200 565 0.0 6 558 7 201 468 0.0 6 460 8 202 404 0.0 6 397 7 203 528 0.0 6 522 6 204 494 0.0 6 485 9 205 496 0.0 6 488 8 206 438 0.0 6 429 9 207 615 0.0 6 598 16 1 208 653 0.0 6 645 7 1 209 549 0.0 6 538 10 0 0 0 1 210 547 0.0 6 539 8 211 415 0.0 6 410 5 212 404 0.0 6 398 6 213 549 0.0 6 538 10 1 214 586 0.0 6 575 10 0 1 215 518 0.0 6 508 10 216 618 0.0 6 612 6 217 821 0.0 6 808 13 218 535 0.0 6 529 6 219 511 0.0 6 504 5 1 1 220 553 0.0 6 548 5 221 524 0.0 6 515 7 1 1 222 528 0.0 6 520 7 1 223 438 0.0 6 436 2 224 431 0.0 6 414 16 0 0 1 225 480 0.0 6 467 13 226 623 0.0 6 613 10 227 760 0.0 6 746 13 1 228 772 0.0 6 756 16 229 657 0.0 6 640 16 1 230 595 0.0 6 583 11 1 231 533 0.0 6 522 10 1 232 641 0.0 6 627 13 0 0 0 1 233 636 0.0 6 631 5 234 666 0.0 6 658 7 1 235 520 0.0 6 507 13 236 436 0.0 6 428 8 237 378 0.0 6 370 7 0 0 0 0 1 238 477 0.0 6 471 6 239 456 0.0 6 451 5 240 542 0.0 6 529 11 1 1 241 653 0.0 6 639 13 1 242 573 0.0 6 552 20 0 0 0 1 243 543 0.0 6 530 11 1 1 244 480 0.0 6 463 15 0 1 0 0 1 245 548 0.0 6 537 10 0 1 246 300 0.0 6 288 12 247 338 0.0 6 333 5 248 315 0.0 6 302 12 0 1 249 482 0.0 6 471 11 250 380 0.0 6 372 8 251 473 0.0 6 464 9 252 440 0.0 6 426 12 1 0 0 1 253 359 0.0 6 345 13 1 254 536 0.0 6 524 12 255 361 0.0 6 354 7 256 307 0.0 6 298 9 257 338 0.0 6 326 11 1 258 389 0.0 6 378 11 259 553 0.0 6 532 19 2 260 740 0.0 6 723 17 261 289 0.0 6 272 15 2 262 574 0.0 6 555 17 2 263 719 0.0 6 703 15 1 264 522 0.0 6 505 16 1 265 668 0.0 6 654 14 266 365 0.0 6 358 7 267 406 0.0 6 388 17 1 268 412 0.0 6 397 15 269 427 0.0 6 413 14 270 236 0.0 6 224 12 271 683 0.0 6 645 38 272 880 0.0 6 856 22 2 273 547 0.0 6 524 22 0 1 274 555 0.0 6 542 13 275 716 0.0 6 688 28 276 1255 0.0 6 1215 40 277 1013 0.0 6 984 26 3 278 613 0.0 6 600 13 279 1062 0.0 6 1043 19 280 1066 0.0 6 978 78 10 281 25114 0.0 6 24692 410 9 1 1 1 282 14 0.0 6 12 1 1 283 4 0.0 6 3 0 0 1 284 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 619 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 15 0.0 6 15 69 1 0.0 6 1 70 2 0.0 6 2 71 7 0.0 6 6 1 72 4 0.0 6 4 73 6 0.0 6 6 74 7 0.0 6 7 75 6 0.0 6 6 76 2 0.0 6 2 77 2 0.0 6 2 78 3 0.0 6 3 79 2 0.0 6 2 80 2 0.0 6 2 81 2 0.0 6 2 82 2 0.0 6 2 83 1 0.0 6 1 84 2 0.0 6 2 85 2 0.0 6 2 86 2 0.0 6 2 87 2 0.0 6 2 88 2 0.0 6 2 89 2 0.0 6 2 90 5 0.0 6 5 91 2 0.0 6 2 92 4 0.0 6 4 93 2 0.0 6 2 94 2 0.0 6 2 95 2 0.0 6 2 96 3 0.0 6 3 97 3 0.0 6 3 98 1 0.0 6 1 99 1 0.0 6 1 100 4 0.0 6 4 101 4 0.0 6 4 102 2 0.0 6 2 103 2 0.0 6 2 104 3 0.0 6 3 105 3 0.0 6 3 106 3 0.0 6 3 107 6 0.0 6 6 108 10 0.0 6 10 110 1 0.0 6 1 111 3 0.0 6 3 112 1 0.0 6 1 113 2 0.0 6 2 115 1 0.0 6 1 116 1 0.0 6 1 117 2 0.0 6 2 118 2 0.0 6 2 121 1 0.0 6 1 122 4 0.0 6 4 123 5 0.0 6 5 124 4 0.0 6 4 125 1 0.0 6 1 126 2 0.0 6 2 127 5 0.0 6 5 128 2 0.0 6 2 129 3 0.0 6 3 130 6 0.0 6 6 131 3 0.0 6 2 0 0 0 0 0 1 132 4 0.0 6 4 133 4 0.0 6 4 134 5 0.0 6 5 135 2 0.0 6 2 136 1 0.0 6 1 137 1 0.0 6 1 138 6 0.0 6 6 139 4 0.0 6 4 140 6 0.0 6 6 141 4 0.0 6 4 143 4 0.0 6 4 144 2 0.0 6 2 145 4 0.0 6 4 146 2 0.0 6 2 147 3 0.0 6 3 148 6 0.0 6 6 149 4 0.0 6 4 150 3 0.0 6 3 151 2 0.0 6 2 152 2 0.0 6 2 153 1 0.0 6 1 154 1 0.0 6 1 155 5 0.0 6 5 156 4 0.0 6 4 157 9 0.0 6 9 158 5 0.0 6 5 159 5 0.0 6 5 160 5 0.0 6 5 161 4 0.0 6 4 162 5 0.0 6 5 163 2 0.0 6 2 164 2 0.0 6 2 165 3 0.0 6 3 166 4 0.0 6 4 167 5 0.0 6 5 168 6 0.0 6 6 169 5 0.0 6 5 170 4 0.0 6 4 171 5 0.0 6 5 172 4 0.0 6 4 173 7 0.0 6 7 174 1 0.0 6 1 175 1 0.0 6 1 176 2 0.0 6 2 177 7 0.0 6 7 178 5 0.0 6 5 179 4 0.0 6 4 180 2 0.0 6 2 181 1 0.0 6 1 182 4 0.0 6 4 183 1 0.0 6 1 184 7 0.0 6 7 185 4 0.0 6 4 186 4 0.0 6 4 187 6 0.0 6 6 188 5 0.0 6 5 189 11 0.0 6 11 190 3 0.0 6 3 191 4 0.0 6 4 192 7 0.0 6 7 193 2 0.0 6 2 194 4 0.0 6 4 195 3 0.0 6 3 196 4 0.0 6 4 197 8 0.0 6 8 198 5 0.0 6 5 199 4 0.0 6 4 200 4 0.0 6 4 201 7 0.0 6 7 202 3 0.0 6 3 203 3 0.0 6 3 204 1 0.0 6 1 205 5 0.0 6 5 206 2 0.0 6 2 207 3 0.0 6 3 208 3 0.0 6 3 209 6 0.0 6 6 210 2 0.0 6 2 211 5 0.0 6 5 212 3 0.0 6 3 213 1 0.0 6 1 214 1 0.0 6 1 215 2 0.0 6 2 216 4 0.0 6 4 217 2 0.0 6 2 218 3 0.0 6 3 219 4 0.0 6 4 220 1 0.0 6 1 221 3 0.0 6 3 222 2 0.0 6 2 223 4 0.0 6 4 224 4 0.0 6 4 225 4 0.0 6 4 226 4 0.0 6 4 227 2 0.0 6 2 229 5 0.0 6 5 230 2 0.0 6 2 231 1 0.0 6 1 232 3 0.0 6 3 234 4 0.0 6 4 235 3 0.0 6 3 236 3 0.0 6 3 237 1 0.0 6 1 238 2 0.0 6 2 239 3 0.0 6 3 240 2 0.0 6 2 241 3 0.0 6 3 242 1 0.0 6 1 244 1 0.0 6 1 245 1 0.0 6 1 247 3 0.0 6 3 248 2 0.0 6 2 250 3 0.0 6 3 251 3 0.0 6 3 252 3 0.0 6 3 254 2 0.0 6 2 255 1 0.0 6 1 256 1 0.0 6 1 257 1 0.0 6 1 259 1 0.0 6 1 260 1 0.0 6 1 262 1 0.0 6 1 265 1 0.0 6 1 266 2 0.0 6 2 267 1 0.0 6 1 268 1 0.0 6 1 269 1 0.0 6 1 276 1 0.0 6 1 277 2 0.0 6 2 281 1 0.0 6 1 282 2 0.0 6 2 284 1 0.0 6 1 288 1 0.0 6 1 291 1 0.0 6 1 292 1 0.0 6 1 297 1 0.0 6 1 301 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 83466 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 328.3 1 1 7 2 5.1 2 2 8 1 1.3 2 1 9 1 0.3 2 1 10 8 0.1 3 7 1 11 8 0.0 3 6 2 12 12 0.0 3 11 0 0 1 13 14 0.0 3 12 2 14 21 0.0 4 20 1 15 4 0.0 4 3 0 0 0 0 1 16 12 0.0 4 9 3 17 47 0.0 5 43 4 18 27 0.0 5 25 2 19 24 0.0 5 23 1 20 69 0.0 6 68 1 21 71 0.0 6 68 3 22 44 0.0 6 42 2 23 43 0.0 6 42 1 24 28 0.0 6 26 2 25 19 0.0 6 16 3 26 19 0.0 6 16 2 1 27 23 0.0 6 20 3 28 21 0.0 6 20 1 29 19 0.0 6 19 30 16 0.0 6 15 1 31 17 0.0 6 16 1 32 11 0.0 6 10 0 1 33 11 0.0 6 10 1 34 16 0.0 6 16 35 11 0.0 6 11 36 9 0.0 6 9 37 16 0.0 6 16 38 22 0.0 6 22 39 13 0.0 6 11 2 40 11 0.0 6 11 41 10 0.0 6 10 42 12 0.0 6 10 0 0 0 2 43 18 0.0 6 16 2 44 35 0.0 6 31 4 45 7 0.0 6 6 1 46 34 0.0 6 29 4 1 47 29 0.0 6 27 2 48 15 0.0 6 12 3 49 28 0.0 6 27 1 50 40 0.0 6 37 3 51 32 0.0 6 23 9 52 41 0.0 6 36 3 0 1 0 1 53 29 0.0 6 28 0 0 1 54 30 0.0 6 25 4 1 55 28 0.0 6 26 2 56 32 0.0 6 28 3 1 57 43 0.0 6 39 4 58 28 0.0 6 26 2 59 24 0.0 6 24 60 20 0.0 6 16 2 2 61 22 0.0 6 22 62 24 0.0 6 23 1 63 19 0.0 6 18 1 64 35 0.0 6 32 3 65 21 0.0 6 21 66 20 0.0 6 19 1 67 55 0.0 6 53 1 0 1 68 158 0.0 6 153 5 69 58 0.0 6 57 0 0 0 1 70 67 0.0 6 63 4 71 41 0.0 6 39 2 72 72 0.0 6 68 3 0 0 0 1 73 34 0.0 6 32 2 74 16 0.0 6 14 1 0 1 75 18 0.0 6 17 1 76 47 0.0 6 42 4 1 77 32 0.0 6 30 2 78 41 0.0 6 40 1 79 41 0.0 6 37 3 0 1 80 47 0.0 6 42 5 81 31 0.0 6 28 3 82 73 0.0 6 66 6 1 83 88 0.0 6 84 3 1 84 93 0.0 6 91 0 2 85 66 0.0 6 61 4 0 0 1 86 43 0.0 6 40 3 87 76 0.0 6 69 7 88 38 0.0 6 36 2 89 56 0.0 6 55 0 0 1 90 57 0.0 6 51 6 91 63 0.0 6 58 4 1 92 57 0.0 6 53 4 93 71 0.0 6 66 4 0 0 0 1 94 142 0.0 6 132 9 0 1 95 65 0.0 6 57 8 96 81 0.0 6 76 4 1 97 89 0.0 6 85 4 98 71 0.0 6 68 2 1 99 78 0.0 6 73 5 100 61 0.0 6 57 4 101 73 0.0 6 65 7 0 1 102 103 0.0 6 94 7 1 0 0 0 1 103 112 0.0 6 102 9 1 104 129 0.0 6 117 11 1 105 178 0.0 6 167 11 106 96 0.0 6 86 8 2 107 116 0.0 6 107 8 0 0 1 108 89 0.0 6 85 3 1 109 81 0.0 6 80 1 110 102 0.0 6 98 4 111 106 0.0 6 104 1 1 112 104 0.0 6 99 5 113 102 0.0 6 99 3 114 135 0.0 6 130 5 115 120 0.0 6 114 6 116 140 0.0 6 138 2 117 101 0.0 6 97 4 118 143 0.0 6 138 5 119 162 0.0 6 159 2 0 0 0 1 120 203 0.0 6 194 6 3 121 146 0.0 6 136 10 122 187 0.0 6 175 12 123 156 0.0 6 153 3 124 221 0.0 6 208 12 0 0 1 125 196 0.0 6 189 5 1 0 0 1 126 193 0.0 6 187 6 127 229 0.0 6 221 7 0 0 0 0 1 128 194 0.0 6 187 5 0 0 0 1 1 129 218 0.0 6 211 7 130 242 0.0 6 236 6 131 297 0.0 6 285 9 1 0 2 132 430 0.0 6 416 10 3 0 1 133 367 0.0 6 354 12 1 134 465 0.0 6 455 7 2 0 1 135 586 0.0 6 565 20 0 1 136 401 0.0 6 388 12 0 0 0 0 1 137 478 0.0 6 461 15 0 0 1 0 1 138 391 0.0 6 382 8 1 139 513 0.0 6 499 13 0 0 0 1 140 624 0.0 6 610 13 0 0 1 141 562 0.0 6 544 15 1 1 0 1 142 669 0.0 6 650 17 1 0 0 0 1 143 663 0.0 6 642 18 1 1 1 144 806 0.0 6 785 18 1 0 1 0 1 145 626 0.0 6 615 9 1 0 1 146 623 0.0 6 612 9 0 1 1 147 567 0.0 6 555 11 0 0 0 0 1 148 673 0.0 6 661 10 0 0 1 1 149 882 0.0 6 862 20 150 1099 0.0 6 1075 22 2 151 1012 0.0 6 995 16 0 0 1 152 1156 0.0 6 1128 25 0 0 1 1 1 153 1010 0.0 6 985 22 0 1 0 0 2 154 1110 0.0 6 1092 17 0 0 1 155 795 0.0 6 776 17 1 1 156 856 0.0 6 841 12 2 0 0 1 157 1057 0.0 6 1031 21 2 0 0 2 1 158 1097 0.0 6 1062 30 1 1 1 0 2 159 792 0.0 6 771 18 1 1 1 160 937 0.0 6 917 17 0 0 2 1 161 821 0.0 6 805 14 1 0 1 162 712 0.0 6 696 15 0 1 163 801 0.0 6 781 17 1 0 0 1 1 164 803 0.0 6 781 20 0 0 2 165 811 0.0 6 788 22 0 1 166 934 0.0 6 924 10 167 698 0.0 6 681 15 1 0 1 168 1305 0.0 6 1272 32 1 169 633 0.0 6 625 7 0 0 0 0 1 170 715 0.0 6 689 25 0 1 171 1079 0.0 6 1057 20 1 0 1 172 617 0.0 6 605 10 0 0 1 0 1 173 643 0.0 6 624 16 1 0 1 1 174 871 0.0 6 845 22 0 2 0 1 1 175 1256 0.0 6 1231 23 0 0 2 176 888 0.0 6 874 13 0 1 177 1017 0.0 6 995 22 178 777 0.0 6 756 21 179 1029 0.0 6 1002 26 1 180 878 0.0 6 859 18 0 1 181 1382 0.0 6 1354 26 2 182 465 0.0 6 445 20 183 1008 0.0 6 995 13 184 975 0.0 6 948 26 0 1 185 958 0.0 6 924 33 0 0 1 186 1845 0.0 6 1803 41 0 0 1 187 1345 0.0 6 1311 31 2 1 188 1305 0.0 6 1278 25 0 1 1 189 1307 0.0 6 1274 28 2 0 1 2 190 1926 0.0 6 1883 40 1 0 2 191 1665 0.0 6 1628 36 1 192 1306 0.0 6 1277 27 0 1 1 193 846 0.0 6 816 27 2 0 1 194 1155 0.0 6 1121 33 1 195 1152 0.0 6 1117 32 0 1 2 196 3280 0.0 6 3201 76 1 2 197 1098 0.0 6 1053 42 2 1 198 1695 0.0 6 1640 52 2 1 199 2540 0.0 6 2467 69 2 0 2 200 651 0.0 6 624 27 201 1059 0.0 6 1016 38 1 1 3 202 483 0.0 6 470 10 2 0 1 203 366 0.0 6 360 5 0 0 1 204 465 0.0 6 459 6 205 205 0.0 6 198 6 1 206 211 0.0 6 208 3 207 140 0.0 6 135 5 208 249 0.0 6 242 6 1 209 237 0.0 6 231 6 210 216 0.0 6 210 5 1 211 225 0.0 6 215 10 212 365 0.0 6 361 4 213 145 0.0 6 139 6 214 197 0.0 6 191 6 215 265 0.0 6 255 10 216 384 0.0 6 366 17 1 217 209 0.0 6 201 8 218 166 0.0 6 161 3 2 219 169 0.0 6 165 4 220 88 0.0 6 84 4 221 95 0.0 6 90 5 222 294 0.0 6 280 14 223 535 0.0 6 522 12 1 224 117 0.0 6 113 4 225 273 0.0 6 270 3 226 154 0.0 6 149 5 227 124 0.0 6 118 6 228 131 0.0 6 126 5 229 129 0.0 6 126 3 230 69 0.0 6 65 4 231 37 0.0 6 35 2 232 194 0.0 6 184 10 233 169 0.0 6 165 4 234 36 0.0 6 35 1 235 29 0.0 6 28 1 236 16 0.0 6 16 237 12 0.0 6 11 1 238 9 0.0 6 9 239 23 0.0 6 21 2 240 55 0.0 6 53 2 241 47 0.0 6 45 2 242 127 0.0 6 127 243 88 0.0 6 84 4 244 53 0.0 6 52 1 245 11 0.0 6 10 1 246 16 0.0 6 14 2 247 8 0.0 6 8 248 16 0.0 6 16 249 14 0.0 6 13 1 250 7 0.0 6 7 251 7 0.0 6 7 252 4 0.0 6 4 253 2 0.0 6 1 1 276 2 0.0 6 2 278 1 0.0 6 1 279 29 0.0 6 28 0 1 280 78 0.0 6 73 3 1 0 1 281 5 0.0 6 4 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 584 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 3 104 1313.3 0 104 52 1 0.0 6 1 58 1 0.0 6 1 59 1 0.0 6 1 60 1 0.0 6 1 61 1 0.0 6 1 68 1 0.0 6 1 70 3 0.0 6 3 71 3 0.0 6 2 1 72 9 0.0 6 9 73 10 0.0 6 10 74 2 0.0 6 2 75 4 0.0 6 4 76 5 0.0 6 5 77 8 0.0 6 8 78 2 0.0 6 2 79 4 0.0 6 4 80 2 0.0 6 2 81 3 0.0 6 3 82 2 0.0 6 2 83 1 0.0 6 1 84 12 0.0 6 12 85 4 0.0 6 4 86 7 0.0 6 7 87 3 0.0 6 3 88 2 0.0 6 2 89 5 0.0 6 5 90 4 0.0 6 4 91 3 0.0 6 3 92 3 0.0 6 3 93 2 0.0 6 2 94 5 0.0 6 5 95 1 0.0 6 1 96 6 0.0 6 6 98 4 0.0 6 4 99 2 0.0 6 2 100 4 0.0 6 4 101 3 0.0 6 3 102 1 0.0 6 1 103 3 0.0 6 3 104 4 0.0 6 4 105 2 0.0 6 2 106 1 0.0 6 1 108 8 0.0 6 8 109 6 0.0 6 6 110 3 0.0 6 3 111 4 0.0 6 4 112 5 0.0 6 5 113 1 0.0 6 1 114 2 0.0 6 2 115 8 0.0 6 8 116 2 0.0 6 2 117 1 0.0 6 1 119 1 0.0 6 1 120 2 0.0 6 2 121 4 0.0 6 4 122 2 0.0 6 2 123 1 0.0 6 1 124 4 0.0 6 4 125 4 0.0 6 4 126 3 0.0 6 3 127 4 0.0 6 4 128 3 0.0 6 3 129 2 0.0 6 2 130 5 0.0 6 5 131 3 0.0 6 3 132 5 0.0 6 5 133 8 0.0 6 8 134 6 0.0 6 5 1 135 4 0.0 6 4 136 1 0.0 6 1 137 5 0.0 6 5 138 8 0.0 6 8 139 3 0.0 6 3 140 3 0.0 6 3 141 3 0.0 6 2 1 142 4 0.0 6 4 143 2 0.0 6 2 144 4 0.0 6 4 145 2 0.0 6 2 146 1 0.0 6 1 147 3 0.0 6 3 148 5 0.0 6 5 149 4 0.0 6 4 150 4 0.0 6 4 151 2 0.0 6 2 152 2 0.0 6 2 153 5 0.0 6 5 154 1 0.0 6 1 155 4 0.0 6 4 156 3 0.0 6 3 157 8 0.0 6 8 158 2 0.0 6 2 159 3 0.0 6 3 160 2 0.0 6 2 161 5 0.0 6 4 1 162 5 0.0 6 5 163 5 0.0 6 5 164 8 0.0 6 8 165 5 0.0 6 5 166 7 0.0 6 7 167 2 0.0 6 2 168 4 0.0 6 4 169 4 0.0 6 4 170 2 0.0 6 2 171 4 0.0 6 4 172 1 0.0 6 1 173 1 0.0 6 1 174 7 0.0 6 7 175 4 0.0 6 4 176 3 0.0 6 3 177 4 0.0 6 4 178 4 0.0 6 4 179 3 0.0 6 3 182 5 0.0 6 5 183 3 0.0 6 3 184 5 0.0 6 5 185 3 0.0 6 3 186 8 0.0 6 8 187 1 0.0 6 1 189 3 0.0 6 3 190 3 0.0 6 3 191 2 0.0 6 2 192 4 0.0 6 4 193 1 0.0 6 1 194 3 0.0 6 3 196 3 0.0 6 3 198 3 0.0 6 3 199 4 0.0 6 4 200 2 0.0 6 2 203 3 0.0 6 3 205 1 0.0 6 1 206 1 0.0 6 1 208 1 0.0 6 1 209 2 0.0 6 2 212 2 0.0 6 2 216 2 0.0 6 2 217 1 0.0 6 1 219 2 0.0 6 1 0 0 0 1 226 1 0.0 6 1 230 1 0.0 6 1 232 1 0.0 6 1 249 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_03_134_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_03_361_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_03_134_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_03_361_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.48 s (57 µs/read; 1.05 M reads/minute). === Summary === Total read pairs processed: 60,880 Read 1 with adapter: 60,864 (100.0%) Read 2 with adapter: 60,828 (99.9%) == Read fate breakdown == Pairs that were too short: 8 (0.0%) Pairs discarded as untrimmed: 57 (0.1%) Pairs written (passing filters): 60,815 (99.9%) Total basepairs processed: 29,731,598 bp Read 1: 16,913,315 bp Read 2: 12,818,283 bp Total written (filtered): 3,800,136 bp (12.8%) Read 1: 1,843,249 bp Read 2: 1,956,887 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 60511 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 951.2 0 0 1 4 2 237.8 1 0 2 5 1 59.5 1 1 6 6 14.9 1 0 0 6 9 1 0.2 2 1 10 2 0.1 3 2 16 1 0.0 4 1 19 1 0.0 5 1 20 36 0.0 6 35 1 21 14 0.0 6 14 22 20 0.0 6 20 23 11 0.0 6 10 1 24 5 0.0 6 5 25 11 0.0 6 11 26 9 0.0 6 9 29 3 0.0 6 3 30 1 0.0 6 1 31 3 0.0 6 1 2 32 3 0.0 6 3 33 5 0.0 6 4 1 34 3 0.0 6 3 35 3 0.0 6 3 36 1 0.0 6 1 37 1 0.0 6 1 38 2 0.0 6 2 39 4 0.0 6 4 40 2 0.0 6 2 41 1 0.0 6 1 42 3 0.0 6 3 43 3 0.0 6 3 44 2 0.0 6 2 45 6 0.0 6 6 46 4 0.0 6 4 48 4 0.0 6 4 49 1 0.0 6 1 50 1 0.0 6 1 51 2 0.0 6 2 52 2 0.0 6 2 53 4 0.0 6 3 0 0 0 0 1 54 4 0.0 6 4 56 5 0.0 6 5 57 3 0.0 6 1 2 58 1 0.0 6 1 59 4 0.0 6 4 61 1 0.0 6 1 62 4 0.0 6 4 64 2 0.0 6 2 65 1 0.0 6 1 66 2 0.0 6 2 67 4 0.0 6 4 68 3 0.0 6 2 1 69 6 0.0 6 6 70 3 0.0 6 3 71 1 0.0 6 1 72 3 0.0 6 3 73 5 0.0 6 5 74 4 0.0 6 4 75 2 0.0 6 2 76 3 0.0 6 3 77 3 0.0 6 3 78 5 0.0 6 5 79 3 0.0 6 3 80 3 0.0 6 3 81 5 0.0 6 5 82 5 0.0 6 4 1 83 5 0.0 6 4 0 1 84 6 0.0 6 6 85 4 0.0 6 4 86 8 0.0 6 8 87 5 0.0 6 4 1 88 1 0.0 6 1 89 7 0.0 6 6 1 90 2 0.0 6 2 91 3 0.0 6 3 92 6 0.0 6 6 93 8 0.0 6 8 94 4 0.0 6 4 95 2 0.0 6 2 96 3 0.0 6 2 1 97 3 0.0 6 3 98 8 0.0 6 7 1 99 2 0.0 6 2 100 5 0.0 6 5 101 5 0.0 6 5 102 11 0.0 6 8 2 1 103 4 0.0 6 4 104 6 0.0 6 5 0 1 105 7 0.0 6 7 106 12 0.0 6 11 0 0 1 107 5 0.0 6 5 108 15 0.0 6 13 2 109 5 0.0 6 4 1 110 5 0.0 6 5 111 8 0.0 6 8 112 9 0.0 6 8 1 113 10 0.0 6 10 114 9 0.0 6 9 115 11 0.0 6 11 116 8 0.0 6 8 117 12 0.0 6 12 118 4 0.0 6 4 119 9 0.0 6 9 120 3 0.0 6 3 121 8 0.0 6 8 122 11 0.0 6 11 123 19 0.0 6 17 2 124 12 0.0 6 11 1 125 16 0.0 6 15 1 126 13 0.0 6 12 1 127 8 0.0 6 8 128 18 0.0 6 18 129 17 0.0 6 17 130 18 0.0 6 18 131 20 0.0 6 19 1 132 22 0.0 6 22 133 19 0.0 6 19 134 10 0.0 6 9 1 135 13 0.0 6 12 1 136 18 0.0 6 18 137 16 0.0 6 16 138 14 0.0 6 13 1 139 23 0.0 6 23 140 24 0.0 6 24 141 18 0.0 6 17 0 1 142 41 0.0 6 39 2 143 149 0.0 6 149 144 215 0.0 6 215 145 304 0.0 6 302 2 146 152 0.0 6 152 147 136 0.0 6 135 1 148 250 0.0 6 249 1 149 218 0.0 6 216 1 1 150 162 0.0 6 162 151 216 0.0 6 216 152 148 0.0 6 148 153 154 0.0 6 154 154 167 0.0 6 167 155 102 0.0 6 102 156 125 0.0 6 124 1 157 121 0.0 6 121 158 110 0.0 6 110 159 71 0.0 6 71 160 139 0.0 6 139 161 108 0.0 6 106 2 162 105 0.0 6 104 1 163 138 0.0 6 138 164 114 0.0 6 113 1 165 113 0.0 6 113 166 140 0.0 6 140 167 102 0.0 6 102 168 85 0.0 6 85 169 108 0.0 6 107 1 170 116 0.0 6 116 171 121 0.0 6 120 1 172 112 0.0 6 110 2 173 100 0.0 6 99 1 174 124 0.0 6 123 1 175 106 0.0 6 105 1 176 107 0.0 6 107 177 102 0.0 6 100 2 178 138 0.0 6 137 1 179 107 0.0 6 106 1 180 140 0.0 6 138 2 181 369 0.0 6 368 1 182 153 0.0 6 153 183 108 0.0 6 107 1 184 138 0.0 6 138 185 137 0.0 6 137 186 104 0.0 6 101 3 187 150 0.0 6 146 3 0 0 1 188 185 0.0 6 183 2 189 154 0.0 6 154 190 177 0.0 6 175 2 191 145 0.0 6 143 2 192 154 0.0 6 150 3 1 193 175 0.0 6 172 3 194 157 0.0 6 155 2 195 178 0.0 6 175 2 0 0 1 196 202 0.0 6 199 3 197 211 0.0 6 206 5 198 251 0.0 6 245 5 1 199 299 0.0 6 295 4 200 291 0.0 6 289 2 201 250 0.0 6 246 3 1 202 260 0.0 6 256 4 203 242 0.0 6 240 2 204 252 0.0 6 248 4 205 316 0.0 6 315 1 206 250 0.0 6 245 5 207 290 0.0 6 287 3 208 358 0.0 6 352 6 209 273 0.0 6 270 2 1 210 271 0.0 6 268 3 211 205 0.0 6 199 6 212 188 0.0 6 188 213 240 0.0 6 239 1 214 247 0.0 6 244 3 215 246 0.0 6 241 5 216 375 0.0 6 370 4 1 217 371 0.0 6 367 4 218 233 0.0 6 233 219 266 0.0 6 263 3 220 268 0.0 6 266 2 221 274 0.0 6 269 4 0 1 222 256 0.0 6 251 5 223 191 0.0 6 191 224 195 0.0 6 191 4 225 289 0.0 6 284 4 0 0 1 226 334 0.0 6 332 2 227 331 0.0 6 324 7 228 305 0.0 6 300 5 229 274 0.0 6 269 4 1 230 220 0.0 6 213 6 1 231 186 0.0 6 183 3 232 219 0.0 6 216 3 233 196 0.0 6 192 4 234 207 0.0 6 206 1 235 212 0.0 6 210 2 236 198 0.0 6 194 4 237 191 0.0 6 188 3 238 203 0.0 6 197 6 239 260 0.0 6 257 3 240 393 0.0 6 385 8 241 381 0.0 6 372 9 242 355 0.0 6 343 12 243 310 0.0 6 302 7 1 244 390 0.0 6 382 8 245 277 0.0 6 266 11 246 190 0.0 6 183 6 1 247 197 0.0 6 194 3 248 224 0.0 6 215 9 249 239 0.0 6 237 2 250 254 0.0 6 250 4 251 238 0.0 6 227 10 0 0 0 0 1 252 224 0.0 6 219 5 253 251 0.0 6 245 5 1 254 218 0.0 6 213 5 255 210 0.0 6 209 0 1 256 188 0.0 6 182 6 257 201 0.0 6 194 5 2 258 239 0.0 6 231 7 1 259 267 0.0 6 262 5 260 248 0.0 6 239 9 261 264 0.0 6 258 6 262 340 0.0 6 325 13 1 0 1 263 215 0.0 6 210 5 264 204 0.0 6 198 6 265 118 0.0 6 115 2 0 1 266 153 0.0 6 151 2 267 157 0.0 6 153 4 268 274 0.0 6 266 8 269 144 0.0 6 141 2 1 270 155 0.0 6 141 12 1 0 0 1 271 636 0.0 6 613 22 1 272 330 0.0 6 320 9 1 273 259 0.0 6 255 4 274 206 0.0 6 201 5 275 379 0.0 6 354 24 1 276 960 0.0 6 923 36 1 277 1047 0.0 6 1020 25 0 2 278 393 0.0 6 380 11 0 1 1 279 892 0.0 6 866 26 280 3873 0.0 6 3690 174 8 1 281 24211 0.0 6 23784 419 2 2 2 1 1 282 9 0.0 6 7 0 1 1 283 2 0.0 6 1 0 0 1 284 3 0.0 6 2 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 353 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 5 0.0 6 0 5 61 9 0.0 6 9 69 1 0.0 6 1 70 4 0.0 6 4 71 4 0.0 6 4 72 4 0.0 6 4 73 2 0.0 6 2 74 4 0.0 6 4 75 8 0.0 6 8 76 2 0.0 6 2 77 4 0.0 6 4 78 1 0.0 6 1 79 2 0.0 6 2 80 5 0.0 6 5 81 2 0.0 6 2 82 3 0.0 6 3 84 1 0.0 6 1 85 1 0.0 6 1 86 1 0.0 6 1 87 3 0.0 6 3 88 1 0.0 6 1 89 3 0.0 6 3 90 1 0.0 6 1 92 3 0.0 6 3 93 3 0.0 6 3 96 4 0.0 6 4 97 1 0.0 6 1 98 2 0.0 6 2 99 1 0.0 6 1 101 2 0.0 6 2 102 2 0.0 6 2 104 1 0.0 6 1 105 3 0.0 6 3 106 4 0.0 6 4 107 5 0.0 6 5 108 8 0.0 6 8 109 2 0.0 6 2 110 2 0.0 6 2 112 1 0.0 6 1 113 2 0.0 6 2 114 4 0.0 6 4 115 3 0.0 6 3 116 3 0.0 6 3 117 4 0.0 6 4 118 4 0.0 6 4 119 1 0.0 6 1 120 1 0.0 6 1 121 1 0.0 6 1 122 2 0.0 6 2 123 3 0.0 6 3 124 3 0.0 6 3 125 2 0.0 6 2 126 2 0.0 6 2 127 2 0.0 6 2 128 2 0.0 6 2 129 2 0.0 6 2 130 1 0.0 6 1 131 1 0.0 6 1 132 2 0.0 6 2 133 2 0.0 6 2 134 3 0.0 6 3 135 2 0.0 6 2 136 2 0.0 6 2 137 2 0.0 6 1 1 138 1 0.0 6 1 139 1 0.0 6 1 140 6 0.0 6 6 141 3 0.0 6 3 142 1 0.0 6 1 143 2 0.0 6 2 144 3 0.0 6 3 145 1 0.0 6 1 147 1 0.0 6 1 148 2 0.0 6 2 149 2 0.0 6 2 150 3 0.0 6 3 152 1 0.0 6 1 153 2 0.0 6 2 154 5 0.0 6 5 155 1 0.0 6 1 156 1 0.0 6 1 157 3 0.0 6 3 158 1 0.0 6 1 159 3 0.0 6 3 160 2 0.0 6 2 161 3 0.0 6 3 162 1 0.0 6 1 163 1 0.0 6 1 164 4 0.0 6 4 165 4 0.0 6 4 167 3 0.0 6 3 168 4 0.0 6 4 169 2 0.0 6 2 170 1 0.0 6 1 171 1 0.0 6 1 172 3 0.0 6 3 173 1 0.0 6 1 174 1 0.0 6 1 175 5 0.0 6 5 176 1 0.0 6 1 177 1 0.0 6 1 178 3 0.0 6 2 0 0 0 0 0 1 179 3 0.0 6 3 180 1 0.0 6 1 181 1 0.0 6 1 183 2 0.0 6 2 185 3 0.0 6 3 186 2 0.0 6 2 187 1 0.0 6 1 188 4 0.0 6 4 189 3 0.0 6 3 191 2 0.0 6 2 193 3 0.0 6 3 195 4 0.0 6 4 196 1 0.0 6 1 197 1 0.0 6 1 198 3 0.0 6 3 200 2 0.0 6 2 201 1 0.0 6 1 202 1 0.0 6 1 203 3 0.0 6 3 204 1 0.0 6 1 205 3 0.0 6 3 207 1 0.0 6 1 208 1 0.0 6 1 209 2 0.0 6 2 212 1 0.0 6 1 214 1 0.0 6 1 215 2 0.0 6 2 216 3 0.0 6 3 217 2 0.0 6 2 219 1 0.0 6 1 220 1 0.0 6 1 221 1 0.0 6 1 222 1 0.0 6 1 224 1 0.0 6 1 226 1 0.0 6 1 227 2 0.0 6 2 229 1 0.0 6 1 230 3 0.0 6 3 231 2 0.0 6 2 232 3 0.0 6 3 235 3 0.0 6 3 236 1 0.0 6 1 239 1 0.0 6 1 240 1 0.0 6 1 242 2 0.0 6 2 243 2 0.0 6 2 244 1 0.0 6 1 246 1 0.0 6 1 253 1 0.0 6 1 255 1 0.0 6 1 256 1 0.0 6 1 258 2 0.0 6 2 260 1 0.0 6 1 270 1 0.0 6 1 271 1 0.0 6 1 272 1 0.0 6 1 287 1 0.0 6 1 288 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 60501 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 3.7 2 1 10 2 0.1 3 2 11 2 0.0 3 2 12 1 0.0 3 1 13 5 0.0 3 5 14 10 0.0 4 7 3 15 1 0.0 4 1 16 13 0.0 4 12 1 17 8 0.0 5 6 1 1 18 17 0.0 5 16 1 19 18 0.0 5 17 1 20 47 0.0 6 43 4 21 33 0.0 6 32 1 22 27 0.0 6 27 23 25 0.0 6 24 1 24 25 0.0 6 25 25 20 0.0 6 18 2 26 17 0.0 6 16 1 27 13 0.0 6 10 3 28 11 0.0 6 10 1 29 5 0.0 6 5 30 15 0.0 6 14 1 31 14 0.0 6 12 2 32 12 0.0 6 10 2 33 8 0.0 6 8 34 8 0.0 6 8 35 11 0.0 6 11 36 1 0.0 6 0 1 37 8 0.0 6 6 1 1 38 7 0.0 6 6 1 39 4 0.0 6 4 40 14 0.0 6 13 1 41 11 0.0 6 10 1 42 6 0.0 6 6 43 13 0.0 6 12 1 44 12 0.0 6 11 1 45 7 0.0 6 6 1 46 19 0.0 6 16 2 0 0 0 1 47 12 0.0 6 12 48 12 0.0 6 11 1 49 6 0.0 6 6 50 14 0.0 6 12 1 1 51 5 0.0 6 5 52 22 0.0 6 15 5 1 0 1 53 13 0.0 6 10 2 1 54 33 0.0 6 27 6 55 24 0.0 6 21 3 56 16 0.0 6 14 2 57 21 0.0 6 17 3 0 0 0 1 58 15 0.0 6 12 2 0 1 59 13 0.0 6 12 0 0 1 60 15 0.0 6 14 1 61 9 0.0 6 6 2 1 62 9 0.0 6 8 0 1 63 14 0.0 6 13 1 64 24 0.0 6 22 2 65 16 0.0 6 15 1 66 19 0.0 6 17 1 0 0 1 67 107 0.0 6 104 3 68 32 0.0 6 30 2 69 47 0.0 6 45 2 70 17 0.0 6 15 1 1 71 34 0.0 6 31 3 72 19 0.0 6 17 2 73 18 0.0 6 18 74 17 0.0 6 15 2 75 22 0.0 6 21 1 76 19 0.0 6 15 1 3 77 12 0.0 6 12 78 23 0.0 6 23 79 22 0.0 6 21 1 80 28 0.0 6 26 1 1 81 29 0.0 6 28 1 82 36 0.0 6 34 2 83 59 0.0 6 57 2 84 34 0.0 6 31 3 85 21 0.0 6 21 86 20 0.0 6 17 3 87 26 0.0 6 24 1 1 88 24 0.0 6 22 2 89 27 0.0 6 25 2 90 29 0.0 6 29 91 30 0.0 6 27 3 92 22 0.0 6 21 1 93 30 0.0 6 28 2 94 66 0.0 6 55 8 1 0 1 1 95 30 0.0 6 27 1 2 96 34 0.0 6 32 2 97 33 0.0 6 32 1 98 51 0.0 6 49 2 99 53 0.0 6 53 100 40 0.0 6 34 6 101 43 0.0 6 39 4 102 55 0.0 6 52 3 103 77 0.0 6 73 4 104 77 0.0 6 72 5 105 59 0.0 6 53 4 0 1 1 106 41 0.0 6 38 2 1 107 66 0.0 6 61 5 108 60 0.0 6 56 4 109 69 0.0 6 64 4 1 110 58 0.0 6 51 7 111 59 0.0 6 55 4 112 58 0.0 6 57 1 113 53 0.0 6 50 3 114 84 0.0 6 80 4 115 71 0.0 6 67 3 0 1 116 73 0.0 6 72 1 117 78 0.0 6 70 8 118 74 0.0 6 70 3 0 0 1 119 69 0.0 6 66 3 120 86 0.0 6 82 2 1 1 121 65 0.0 6 63 2 122 84 0.0 6 79 4 1 123 78 0.0 6 73 5 124 105 0.0 6 98 7 125 97 0.0 6 94 3 126 97 0.0 6 95 2 127 162 0.0 6 152 9 0 0 0 0 1 128 118 0.0 6 110 7 0 1 129 125 0.0 6 124 1 130 98 0.0 6 97 1 131 156 0.0 6 147 8 0 1 132 181 0.0 6 173 6 2 133 187 0.0 6 183 3 1 134 238 0.0 6 235 3 135 302 0.0 6 292 9 1 136 200 0.0 6 196 4 137 214 0.0 6 212 2 138 233 0.0 6 230 3 139 371 0.0 6 363 7 0 0 1 140 498 0.0 6 484 14 141 371 0.0 6 365 4 1 1 142 393 0.0 6 385 8 143 426 0.0 6 422 4 144 509 0.0 6 500 8 0 1 145 373 0.0 6 369 4 146 432 0.0 6 424 5 2 0 1 147 369 0.0 6 364 5 148 437 0.0 6 425 11 0 1 149 554 0.0 6 527 25 1 0 0 1 150 791 0.0 6 776 14 1 151 429 0.0 6 417 11 0 0 0 0 1 152 766 0.0 6 753 12 1 153 558 0.0 6 549 8 1 154 466 0.0 6 455 10 0 0 0 1 155 519 0.0 6 508 10 0 0 1 156 439 0.0 6 426 11 0 0 1 0 1 157 532 0.0 6 525 6 1 158 531 0.0 6 525 6 159 374 0.0 6 367 7 160 453 0.0 6 445 7 1 161 373 0.0 6 359 13 0 0 0 1 162 288 0.0 6 282 5 1 163 449 0.0 6 440 8 0 1 164 406 0.0 6 394 12 165 492 0.0 6 474 17 0 0 1 166 533 0.0 6 522 10 0 0 1 167 395 0.0 6 388 7 168 601 0.0 6 597 3 0 1 169 339 0.0 6 331 6 0 0 2 170 337 0.0 6 328 8 0 0 0 1 171 533 0.0 6 518 14 0 1 172 431 0.0 6 420 11 173 556 0.0 6 542 13 0 0 0 0 1 174 760 0.0 6 737 21 0 2 175 887 0.0 6 868 18 0 0 0 0 1 176 799 0.0 6 788 10 0 1 177 591 0.0 6 570 19 2 178 506 0.0 6 489 16 0 0 0 1 179 513 0.0 6 494 19 180 452 0.0 6 441 9 0 1 1 181 477 0.0 6 472 4 1 182 246 0.0 6 233 12 1 183 628 0.0 6 613 14 0 0 0 1 184 505 0.0 6 491 12 1 0 0 1 185 719 0.0 6 702 15 1 0 1 186 1013 0.0 6 983 29 0 0 0 1 187 914 0.0 6 889 21 1 0 3 188 905 0.0 6 886 19 189 823 0.0 6 794 29 190 1241 0.0 6 1211 27 1 1 0 0 1 191 1396 0.0 6 1358 34 1 2 1 192 700 0.0 6 678 22 193 434 0.0 6 418 13 2 0 1 194 895 0.0 6 873 19 2 0 1 195 1055 0.0 6 1005 47 2 1 196 2115 0.0 6 2064 46 3 1 1 197 771 0.0 6 739 30 1 1 198 1399 0.0 6 1365 34 199 2104 0.0 6 2060 44 200 826 0.0 6 801 24 0 0 1 201 1169 0.0 6 1123 45 0 1 202 582 0.0 6 566 16 203 533 0.0 6 511 22 204 824 0.0 6 795 28 0 0 1 205 286 0.0 6 274 12 206 352 0.0 6 346 6 207 203 0.0 6 194 7 1 1 208 376 0.0 6 368 8 209 400 0.0 6 390 9 0 1 210 363 0.0 6 352 9 2 211 299 0.0 6 281 17 0 0 1 212 515 0.0 6 506 8 0 0 0 1 213 287 0.0 6 275 11 0 0 0 0 1 214 1036 0.0 6 1012 24 215 499 0.0 6 490 9 216 362 0.0 6 360 2 217 463 0.0 6 450 12 0 1 218 228 0.0 6 220 8 219 361 0.0 6 348 11 0 1 1 220 88 0.0 6 83 5 221 325 0.0 6 312 12 1 222 390 0.0 6 384 5 1 223 514 0.0 6 500 14 224 304 0.0 6 297 7 225 323 0.0 6 316 6 1 226 296 0.0 6 288 7 0 0 1 227 269 0.0 6 262 7 228 116 0.0 6 115 1 229 330 0.0 6 325 4 1 230 151 0.0 6 147 4 231 311 0.0 6 304 7 232 358 0.0 6 350 8 233 216 0.0 6 213 3 234 115 0.0 6 111 4 235 67 0.0 6 65 2 236 22 0.0 6 22 237 17 0.0 6 17 238 24 0.0 6 24 239 36 0.0 6 32 4 240 93 0.0 6 91 2 241 163 0.0 6 160 3 242 204 0.0 6 198 6 243 176 0.0 6 173 3 244 117 0.0 6 116 1 245 57 0.0 6 57 246 88 0.0 6 86 2 247 21 0.0 6 21 248 89 0.0 6 88 1 249 14 0.0 6 13 1 250 15 0.0 6 15 251 28 0.0 6 28 252 5 0.0 6 4 1 253 3 0.0 6 3 254 1 0.0 6 1 255 1 0.0 6 1 257 1 0.0 6 1 258 1 0.0 6 1 277 2 0.0 6 2 278 24 0.0 6 22 2 279 46 0.0 6 46 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 327 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 52 3 0.0 6 3 53 2 0.0 6 2 56 1 0.0 6 1 58 1 0.0 6 1 60 1 0.0 6 1 69 1 0.0 6 1 70 1 0.0 6 1 72 11 0.0 6 11 73 9 0.0 6 9 74 9 0.0 6 9 75 4 0.0 6 4 76 6 0.0 6 6 77 5 0.0 6 5 78 2 0.0 6 2 79 6 0.0 6 6 80 7 0.0 6 7 81 3 0.0 6 3 82 7 0.0 6 7 83 7 0.0 6 7 84 1 0.0 6 1 85 6 0.0 6 6 86 1 0.0 6 1 87 1 0.0 6 1 89 1 0.0 6 1 90 3 0.0 6 3 91 4 0.0 6 4 92 4 0.0 6 4 93 1 0.0 6 1 94 2 0.0 6 2 95 3 0.0 6 3 96 2 0.0 6 2 97 3 0.0 6 3 99 1 0.0 6 1 100 2 0.0 6 2 101 3 0.0 6 3 102 4 0.0 6 3 1 103 3 0.0 6 3 104 2 0.0 6 2 105 1 0.0 6 1 106 1 0.0 6 1 107 3 0.0 6 3 108 2 0.0 6 2 109 7 0.0 6 7 110 7 0.0 6 7 112 4 0.0 6 4 113 2 0.0 6 2 115 2 0.0 6 2 116 2 0.0 6 2 117 3 0.0 6 3 118 3 0.0 6 3 119 1 0.0 6 1 121 2 0.0 6 1 0 0 0 1 122 1 0.0 6 1 123 1 0.0 6 1 125 3 0.0 6 3 126 2 0.0 6 2 127 3 0.0 6 3 128 3 0.0 6 3 129 2 0.0 6 2 131 1 0.0 6 1 132 2 0.0 6 2 133 3 0.0 6 3 134 4 0.0 6 3 0 0 1 135 2 0.0 6 2 136 1 0.0 6 1 137 1 0.0 6 1 138 2 0.0 6 2 139 1 0.0 6 1 141 5 0.0 6 5 142 1 0.0 6 1 143 5 0.0 6 5 144 1 0.0 6 1 146 1 0.0 6 1 147 1 0.0 6 1 148 4 0.0 6 4 149 4 0.0 6 4 150 2 0.0 6 2 151 1 0.0 6 1 152 5 0.0 6 5 153 1 0.0 6 1 154 3 0.0 6 3 155 3 0.0 6 3 156 2 0.0 6 1 1 157 1 0.0 6 1 158 3 0.0 6 3 159 5 0.0 6 5 160 2 0.0 6 2 161 1 0.0 6 1 162 5 0.0 6 5 163 5 0.0 6 5 164 2 0.0 6 2 165 1 0.0 6 1 166 2 0.0 6 2 167 5 0.0 6 5 168 4 0.0 6 4 169 2 0.0 6 2 170 2 0.0 6 2 171 1 0.0 6 1 173 2 0.0 6 2 175 2 0.0 6 2 176 4 0.0 6 4 177 2 0.0 6 1 0 0 0 1 178 1 0.0 6 1 180 1 0.0 6 1 181 2 0.0 6 2 182 2 0.0 6 2 183 1 0.0 6 1 184 1 0.0 6 1 185 1 0.0 6 1 186 1 0.0 6 1 187 1 0.0 6 1 188 2 0.0 6 1 1 189 3 0.0 6 3 191 2 0.0 6 2 193 1 0.0 6 1 194 1 0.0 6 1 196 1 0.0 6 1 197 1 0.0 6 1 199 1 0.0 6 1 201 1 0.0 6 0 1 203 1 0.0 6 1 204 2 0.0 6 2 206 1 0.0 6 1 207 1 0.0 6 1 208 1 0.0 6 1 213 1 0.0 6 1 220 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_04_135_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_04_362_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_04_135_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_04_362_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.50 s (22675 µs/read; 0.00 M reads/minute). === Summary === Total read pairs processed: 22 Read 1 with adapter: 22 (100.0%) Read 2 with adapter: 22 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 0 (0.0%) Pairs written (passing filters): 22 (100.0%) Total basepairs processed: 9,425 bp Read 1: 5,675 bp Read 2: 3,750 bp Total written (filtered): 1,298 bp (13.8%) Read 1: 660 bp Read 2: 638 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 22 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 129 1 0.0 6 1 142 1 0.0 6 1 205 1 0.0 6 1 207 1 0.0 6 1 210 1 0.0 6 1 212 2 0.0 6 2 215 2 0.0 6 2 216 1 0.0 6 1 219 1 0.0 6 1 230 1 0.0 6 1 240 2 0.0 6 2 242 1 0.0 6 1 249 1 0.0 6 1 259 1 0.0 6 1 269 1 0.0 6 1 270 1 0.0 6 1 274 1 0.0 6 1 280 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 0 times === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 22 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 95.5% G: 0.0% T: 4.5% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 20 1 0.0 6 1 47 1 0.0 6 1 86 1 0.0 6 1 87 1 0.0 6 1 99 1 0.0 6 1 102 1 0.0 6 1 108 1 0.0 6 1 131 1 0.0 6 1 138 1 0.0 6 1 139 1 0.0 6 1 155 1 0.0 6 1 158 1 0.0 6 1 159 1 0.0 6 1 167 1 0.0 6 1 168 1 0.0 6 1 182 1 0.0 6 1 187 1 0.0 6 1 190 1 0.0 6 0 0 0 0 0 0 1 191 1 0.0 6 1 192 1 0.0 6 1 198 1 0.0 6 1 208 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 0 times WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_05_136_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_05_363_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_05_136_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_05_363_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.45 s (13537 µs/read; 0.00 M reads/minute). === Summary === Total read pairs processed: 33 Read 1 with adapter: 33 (100.0%) Read 2 with adapter: 33 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 0 (0.0%) Pairs written (passing filters): 33 (100.0%) Total basepairs processed: 14,817 bp Read 1: 8,262 bp Read 2: 6,555 bp Total written (filtered): 1,977 bp (13.3%) Read 1: 990 bp Read 2: 987 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 33 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 41 1 0.0 6 1 126 1 0.0 6 1 150 1 0.0 6 1 165 1 0.0 6 1 188 1 0.0 6 1 191 1 0.0 6 1 198 1 0.0 6 1 201 2 0.0 6 2 202 1 0.0 6 1 204 1 0.0 6 1 208 1 0.0 6 1 210 1 0.0 6 1 217 1 0.0 6 1 218 1 0.0 6 1 222 2 0.0 6 2 223 1 0.0 6 1 224 1 0.0 6 1 237 1 0.0 6 1 243 1 0.0 6 1 246 1 0.0 6 1 248 1 0.0 6 1 252 1 0.0 6 1 257 1 0.0 6 1 260 2 0.0 6 2 269 1 0.0 6 1 272 1 0.0 6 1 277 1 0.0 6 1 279 1 0.0 6 1 280 1 0.0 6 1 281 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 0 times === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 33 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 57 1 0.0 6 1 93 1 0.0 6 1 109 1 0.0 6 1 112 1 0.0 6 1 141 1 0.0 6 1 144 1 0.0 6 1 145 1 0.0 6 1 148 1 0.0 6 1 149 1 0.0 6 1 150 1 0.0 6 1 153 1 0.0 6 1 155 1 0.0 6 1 157 1 0.0 6 1 159 1 0.0 6 1 162 2 0.0 6 1 1 169 2 0.0 6 2 176 1 0.0 6 1 177 2 0.0 6 2 179 1 0.0 6 1 185 1 0.0 6 1 187 1 0.0 6 1 188 1 0.0 6 1 200 1 0.0 6 1 205 1 0.0 6 1 214 1 0.0 6 0 1 220 1 0.0 6 1 222 1 0.0 6 1 224 1 0.0 6 1 230 1 0.0 6 1 250 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 0 times WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_06_137_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_06_364_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_06_137_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_06_364_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.70 s (41 µs/read; 1.46 M reads/minute). === Summary === Total read pairs processed: 65,629 Read 1 with adapter: 65,619 (100.0%) Read 2 with adapter: 65,607 (100.0%) == Read fate breakdown == Pairs that were too short: 30 (0.0%) Pairs discarded as untrimmed: 32 (0.0%) Pairs written (passing filters): 65,567 (99.9%) Total basepairs processed: 28,339,746 bp Read 1: 16,069,881 bp Read 2: 12,269,865 bp Total written (filtered): 3,954,541 bp (14.0%) Read 1: 2,026,363 bp Read 2: 1,928,178 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 64242 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1025.5 0 2 4 1 256.4 1 0 1 7 1 4.0 2 0 1 8 1 1.0 2 1 9 4 0.3 2 3 1 10 1 0.1 3 1 15 1 0.0 4 1 16 1 0.0 4 1 17 1 0.0 5 1 19 2 0.0 5 0 2 20 89 0.0 6 87 2 21 70 0.0 6 67 3 22 61 0.0 6 61 23 45 0.0 6 44 1 24 25 0.0 6 25 25 18 0.0 6 17 0 0 0 1 26 22 0.0 6 21 1 27 14 0.0 6 14 28 14 0.0 6 13 1 29 13 0.0 6 12 1 30 7 0.0 6 7 31 5 0.0 6 5 32 3 0.0 6 3 33 4 0.0 6 4 34 7 0.0 6 6 1 35 6 0.0 6 6 36 4 0.0 6 4 37 4 0.0 6 4 38 2 0.0 6 1 1 39 7 0.0 6 7 40 9 0.0 6 8 1 41 6 0.0 6 6 42 4 0.0 6 4 43 6 0.0 6 6 44 5 0.0 6 5 45 5 0.0 6 4 0 1 46 5 0.0 6 5 47 8 0.0 6 8 48 4 0.0 6 3 1 49 8 0.0 6 8 50 6 0.0 6 5 1 51 4 0.0 6 3 1 52 8 0.0 6 7 1 53 5 0.0 6 5 54 6 0.0 6 6 55 8 0.0 6 6 2 56 4 0.0 6 3 0 0 0 1 57 5 0.0 6 5 58 1 0.0 6 1 59 2 0.0 6 1 1 60 5 0.0 6 5 61 6 0.0 6 5 1 62 4 0.0 6 4 63 5 0.0 6 5 64 4 0.0 6 3 1 65 7 0.0 6 7 66 7 0.0 6 7 67 9 0.0 6 9 68 6 0.0 6 4 2 69 5 0.0 6 5 70 6 0.0 6 6 71 9 0.0 6 8 1 72 10 0.0 6 9 1 73 12 0.0 6 12 74 15 0.0 6 15 75 8 0.0 6 8 76 14 0.0 6 14 77 5 0.0 6 5 78 7 0.0 6 6 1 79 7 0.0 6 7 80 7 0.0 6 7 81 8 0.0 6 8 82 8 0.0 6 8 83 10 0.0 6 10 84 11 0.0 6 11 85 12 0.0 6 12 86 13 0.0 6 13 87 13 0.0 6 13 88 5 0.0 6 5 89 12 0.0 6 11 1 90 9 0.0 6 9 91 14 0.0 6 14 92 9 0.0 6 9 93 8 0.0 6 8 94 15 0.0 6 13 1 0 0 0 1 95 6 0.0 6 6 96 8 0.0 6 7 1 97 11 0.0 6 11 98 7 0.0 6 5 2 99 10 0.0 6 10 100 8 0.0 6 8 101 9 0.0 6 8 1 102 14 0.0 6 14 103 17 0.0 6 17 104 17 0.0 6 15 1 1 105 17 0.0 6 17 106 12 0.0 6 12 107 23 0.0 6 22 1 108 21 0.0 6 20 1 109 14 0.0 6 13 1 110 9 0.0 6 9 111 17 0.0 6 17 112 12 0.0 6 12 113 14 0.0 6 14 114 22 0.0 6 22 115 14 0.0 6 14 116 18 0.0 6 18 117 16 0.0 6 14 2 118 16 0.0 6 16 119 21 0.0 6 19 2 120 18 0.0 6 17 1 121 24 0.0 6 24 122 16 0.0 6 16 123 19 0.0 6 19 124 25 0.0 6 25 125 24 0.0 6 24 126 28 0.0 6 28 127 31 0.0 6 31 128 24 0.0 6 23 1 129 22 0.0 6 22 130 24 0.0 6 24 131 30 0.0 6 29 1 132 28 0.0 6 25 3 133 30 0.0 6 30 134 18 0.0 6 17 1 135 33 0.0 6 31 2 136 46 0.0 6 45 0 0 1 137 37 0.0 6 36 1 138 39 0.0 6 37 1 0 0 0 0 1 139 33 0.0 6 33 140 102 0.0 6 102 141 193 0.0 6 192 1 142 277 0.0 6 277 143 251 0.0 6 251 144 225 0.0 6 225 145 279 0.0 6 279 146 231 0.0 6 231 147 239 0.0 6 239 148 306 0.0 6 305 1 149 213 0.0 6 213 150 301 0.0 6 300 1 151 235 0.0 6 235 152 150 0.0 6 150 153 210 0.0 6 208 2 154 224 0.0 6 224 155 284 0.0 6 282 2 156 187 0.0 6 185 1 1 157 250 0.0 6 249 1 158 216 0.0 6 214 2 159 275 0.0 6 273 2 160 443 0.0 6 440 3 161 371 0.0 6 368 3 162 222 0.0 6 220 2 163 220 0.0 6 220 164 237 0.0 6 235 1 0 0 0 1 165 204 0.0 6 202 2 166 229 0.0 6 227 2 167 298 0.0 6 296 2 168 216 0.0 6 215 1 169 237 0.0 6 236 0 0 1 170 270 0.0 6 270 171 239 0.0 6 236 2 0 0 1 172 321 0.0 6 318 3 173 251 0.0 6 251 174 260 0.0 6 256 4 175 229 0.0 6 228 1 176 185 0.0 6 182 2 0 0 0 0 1 177 243 0.0 6 239 2 0 0 1 1 178 445 0.0 6 443 2 179 265 0.0 6 263 1 0 0 0 1 180 224 0.0 6 224 181 292 0.0 6 291 1 182 242 0.0 6 239 2 0 0 0 1 183 225 0.0 6 221 4 184 258 0.0 6 257 0 1 185 315 0.0 6 311 4 186 271 0.0 6 271 187 319 0.0 6 317 2 188 304 0.0 6 301 3 189 279 0.0 6 277 1 0 1 190 290 0.0 6 287 2 0 0 1 191 332 0.0 6 331 1 192 315 0.0 6 314 1 193 435 0.0 6 434 1 194 471 0.0 6 468 3 195 374 0.0 6 370 4 196 549 0.0 6 547 2 197 553 0.0 6 550 3 198 631 0.0 6 622 8 0 0 0 0 1 199 697 0.0 6 688 5 2 1 0 0 1 200 747 0.0 6 741 5 1 201 574 0.0 6 569 5 202 762 0.0 6 760 2 203 560 0.0 6 558 2 204 503 0.0 6 497 5 0 0 0 0 1 205 585 0.0 6 579 4 0 0 1 1 206 551 0.0 6 547 4 207 621 0.0 6 616 5 208 758 0.0 6 753 5 209 656 0.0 6 652 4 210 799 0.0 6 795 4 211 632 0.0 6 625 7 212 635 0.0 6 628 7 213 736 0.0 6 732 4 214 740 0.0 6 737 3 215 677 0.0 6 672 4 1 216 862 0.0 6 857 5 217 989 0.0 6 983 3 0 1 2 218 758 0.0 6 748 8 0 1 0 0 1 219 822 0.0 6 815 6 1 220 847 0.0 6 842 4 0 1 221 715 0.0 6 713 2 222 646 0.0 6 643 3 223 673 0.0 6 668 4 0 0 0 0 1 224 815 0.0 6 810 5 225 711 0.0 6 708 3 226 806 0.0 6 794 9 0 2 0 0 1 227 763 0.0 6 757 3 0 1 0 1 1 228 765 0.0 6 759 5 0 0 0 1 229 654 0.0 6 650 3 1 230 624 0.0 6 619 5 231 610 0.0 6 603 5 1 1 232 661 0.0 6 654 5 1 0 0 1 233 547 0.0 6 543 3 0 0 0 0 1 234 577 0.0 6 569 8 235 640 0.0 6 637 3 236 563 0.0 6 557 5 1 237 526 0.0 6 524 2 238 575 0.0 6 571 4 239 537 0.0 6 532 4 0 0 0 1 240 587 0.0 6 585 1 0 0 0 1 241 569 0.0 6 564 5 242 568 0.0 6 567 1 243 542 0.0 6 540 2 244 578 0.0 6 571 6 0 1 245 624 0.0 6 618 5 0 1 246 496 0.0 6 493 3 247 568 0.0 6 566 2 248 455 0.0 6 453 2 249 390 0.0 6 390 250 366 0.0 6 364 2 251 371 0.0 6 368 3 252 396 0.0 6 394 1 1 253 376 0.0 6 372 3 0 0 0 1 254 330 0.0 6 328 2 255 344 0.0 6 341 3 256 339 0.0 6 338 1 257 341 0.0 6 338 3 258 290 0.0 6 290 259 268 0.0 6 263 4 0 0 1 260 285 0.0 6 285 261 330 0.0 6 327 3 262 310 0.0 6 309 1 263 244 0.0 6 242 2 264 232 0.0 6 230 2 265 222 0.0 6 222 266 235 0.0 6 235 267 201 0.0 6 200 0 0 0 0 1 268 227 0.0 6 225 2 269 215 0.0 6 214 1 270 236 0.0 6 235 1 271 216 0.0 6 215 1 272 190 0.0 6 190 273 179 0.0 6 178 1 274 165 0.0 6 162 2 0 0 0 0 1 275 199 0.0 6 199 276 251 0.0 6 250 0 0 1 277 286 0.0 6 284 1 1 278 338 0.0 6 338 279 439 0.0 6 436 3 280 1431 0.0 6 1420 9 0 1 1 281 2610 0.0 6 2597 12 1 282 17 0.0 6 16 1 284 1 0.0 6 1 286 1 0.0 6 1 288 1 0.0 6 1 289 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1377 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 33 0.0 6 32 1 63 1 0.0 6 1 68 1 0.0 6 1 69 1 0.0 6 1 70 4 0.0 6 4 71 6 0.0 6 6 72 10 0.0 6 10 73 9 0.0 6 9 74 5 0.0 6 5 75 7 0.0 6 7 76 10 0.0 6 10 77 5 0.0 6 5 78 7 0.0 6 7 79 3 0.0 6 3 80 15 0.0 6 15 81 3 0.0 6 2 1 82 4 0.0 6 4 83 7 0.0 6 7 84 8 0.0 6 8 85 10 0.0 6 10 86 4 0.0 6 4 87 7 0.0 6 7 88 2 0.0 6 2 89 5 0.0 6 5 90 9 0.0 6 9 91 6 0.0 6 6 92 7 0.0 6 7 93 4 0.0 6 4 94 3 0.0 6 3 95 3 0.0 6 3 96 5 0.0 6 5 97 5 0.0 6 5 98 3 0.0 6 3 99 5 0.0 6 5 100 6 0.0 6 6 101 5 0.0 6 5 102 6 0.0 6 6 103 5 0.0 6 5 104 4 0.0 6 4 105 4 0.0 6 4 106 4 0.0 6 4 107 6 0.0 6 6 108 7 0.0 6 7 109 4 0.0 6 4 110 4 0.0 6 4 111 1 0.0 6 1 112 5 0.0 6 5 113 3 0.0 6 3 114 7 0.0 6 7 115 7 0.0 6 7 116 3 0.0 6 3 117 2 0.0 6 2 118 3 0.0 6 3 119 2 0.0 6 2 120 3 0.0 6 3 121 1 0.0 6 1 122 5 0.0 6 5 123 8 0.0 6 8 124 6 0.0 6 6 125 7 0.0 6 7 126 11 0.0 6 11 127 3 0.0 6 3 128 2 0.0 6 2 129 6 0.0 6 6 130 2 0.0 6 2 131 7 0.0 6 7 132 20 0.0 6 20 133 3 0.0 6 3 135 6 0.0 6 6 137 4 0.0 6 4 138 2 0.0 6 2 139 12 0.0 6 12 140 15 0.0 6 15 141 11 0.0 6 11 142 8 0.0 6 7 1 143 6 0.0 6 6 144 7 0.0 6 7 145 3 0.0 6 3 146 6 0.0 6 6 147 10 0.0 6 10 148 6 0.0 6 6 149 7 0.0 6 7 150 3 0.0 6 3 151 7 0.0 6 7 152 7 0.0 6 7 153 5 0.0 6 5 154 6 0.0 6 6 155 7 0.0 6 7 156 4 0.0 6 4 157 10 0.0 6 10 158 2 0.0 6 2 159 7 0.0 6 7 160 12 0.0 6 12 161 10 0.0 6 10 162 7 0.0 6 7 163 5 0.0 6 5 164 4 0.0 6 4 165 4 0.0 6 4 166 6 0.0 6 6 167 7 0.0 6 7 168 10 0.0 6 10 169 12 0.0 6 12 170 11 0.0 6 11 171 9 0.0 6 9 172 7 0.0 6 7 173 4 0.0 6 4 174 9 0.0 6 9 175 17 0.0 6 17 176 13 0.0 6 13 177 11 0.0 6 11 178 5 0.0 6 5 179 4 0.0 6 4 180 5 0.0 6 5 181 4 0.0 6 4 182 11 0.0 6 11 183 13 0.0 6 13 184 5 0.0 6 5 185 4 0.0 6 4 186 20 0.0 6 20 187 20 0.0 6 20 188 15 0.0 6 15 189 10 0.0 6 10 190 4 0.0 6 4 191 6 0.0 6 6 192 7 0.0 6 7 193 14 0.0 6 14 194 9 0.0 6 9 195 11 0.0 6 11 196 3 0.0 6 3 197 9 0.0 6 9 198 4 0.0 6 4 199 7 0.0 6 7 200 9 0.0 6 9 201 9 0.0 6 9 202 3 0.0 6 3 203 7 0.0 6 7 204 10 0.0 6 10 205 10 0.0 6 10 206 8 0.0 6 8 207 8 0.0 6 8 208 8 0.0 6 8 209 6 0.0 6 6 210 29 0.0 6 29 211 32 0.0 6 32 212 7 0.0 6 7 213 5 0.0 6 5 214 4 0.0 6 4 215 9 0.0 6 9 216 8 0.0 6 8 217 9 0.0 6 9 218 5 0.0 6 5 219 9 0.0 6 9 220 8 0.0 6 8 221 9 0.0 6 9 222 7 0.0 6 7 223 3 0.0 6 3 224 9 0.0 6 9 225 9 0.0 6 9 226 9 0.0 6 9 227 5 0.0 6 5 228 3 0.0 6 3 229 8 0.0 6 8 230 7 0.0 6 7 231 5 0.0 6 5 232 7 0.0 6 7 233 3 0.0 6 3 234 4 0.0 6 4 235 4 0.0 6 4 236 8 0.0 6 8 237 6 0.0 6 6 238 3 0.0 6 3 239 4 0.0 6 4 240 8 0.0 6 8 241 4 0.0 6 4 242 12 0.0 6 12 243 12 0.0 6 12 244 3 0.0 6 3 245 6 0.0 6 6 246 12 0.0 6 12 247 12 0.0 6 12 248 7 0.0 6 7 249 3 0.0 6 3 252 6 0.0 6 6 253 4 0.0 6 4 254 2 0.0 6 2 255 1 0.0 6 1 256 3 0.0 6 3 257 4 0.0 6 4 258 4 0.0 6 4 259 2 0.0 6 2 260 4 0.0 6 3 1 261 5 0.0 6 5 262 1 0.0 6 1 263 3 0.0 6 3 264 3 0.0 6 3 265 2 0.0 6 2 267 2 0.0 6 2 268 1 0.0 6 1 269 3 0.0 6 3 270 2 0.0 6 2 271 2 0.0 6 2 273 3 0.0 6 3 274 3 0.0 6 3 275 2 0.0 6 2 276 2 0.0 6 2 277 1 0.0 6 1 278 2 0.0 6 2 279 2 0.0 6 2 280 1 0.0 6 1 284 2 0.0 6 2 285 2 0.0 6 2 286 3 0.0 6 3 288 3 0.0 6 3 291 1 0.0 6 1 295 1 0.0 6 1 297 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 64651 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 16.0 1 1 7 3 4.0 2 3 9 1 0.3 2 1 10 1 0.1 3 0 1 11 4 0.0 3 2 2 12 3 0.0 3 3 13 5 0.0 3 3 1 0 0 0 1 14 11 0.0 4 11 15 2 0.0 4 2 16 3 0.0 4 3 17 26 0.0 5 24 1 1 18 5 0.0 5 5 19 78 0.0 5 75 1 2 20 105 0.0 6 102 2 1 21 57 0.0 6 55 2 22 40 0.0 6 37 3 23 26 0.0 6 26 24 27 0.0 6 27 25 20 0.0 6 19 1 26 26 0.0 6 24 2 27 31 0.0 6 28 3 28 12 0.0 6 10 2 29 24 0.0 6 20 4 30 9 0.0 6 8 1 31 23 0.0 6 22 1 32 16 0.0 6 16 33 18 0.0 6 15 2 1 34 6 0.0 6 4 1 0 0 1 35 12 0.0 6 11 1 36 18 0.0 6 18 37 9 0.0 6 7 2 38 9 0.0 6 9 39 11 0.0 6 11 40 12 0.0 6 11 1 41 9 0.0 6 8 0 1 42 17 0.0 6 14 1 0 0 1 1 43 9 0.0 6 8 1 44 11 0.0 6 11 45 20 0.0 6 18 1 1 46 13 0.0 6 12 1 47 22 0.0 6 21 1 48 15 0.0 6 14 0 1 49 18 0.0 6 18 50 24 0.0 6 23 1 51 27 0.0 6 24 2 0 1 52 23 0.0 6 20 2 1 53 19 0.0 6 18 1 54 20 0.0 6 16 3 1 55 24 0.0 6 22 1 1 56 28 0.0 6 24 2 1 0 1 57 24 0.0 6 22 2 58 27 0.0 6 25 2 59 24 0.0 6 22 2 60 30 0.0 6 28 2 61 22 0.0 6 20 2 62 34 0.0 6 30 3 0 1 63 27 0.0 6 27 64 24 0.0 6 21 3 65 36 0.0 6 33 2 0 1 66 41 0.0 6 39 0 0 1 0 0 1 67 29 0.0 6 26 1 1 0 0 0 1 68 39 0.0 6 38 1 69 63 0.0 6 60 1 1 1 70 133 0.0 6 130 3 71 52 0.0 6 50 2 72 57 0.0 6 55 0 0 1 1 73 47 0.0 6 46 1 74 45 0.0 6 42 2 0 0 1 75 31 0.0 6 29 2 76 47 0.0 6 43 2 1 1 77 49 0.0 6 48 1 78 51 0.0 6 46 3 1 0 0 1 79 50 0.0 6 48 2 80 43 0.0 6 39 2 1 1 81 54 0.0 6 49 2 2 0 0 0 1 82 47 0.0 6 41 5 1 83 83 0.0 6 80 3 84 64 0.0 6 60 3 0 0 1 85 76 0.0 6 69 6 0 1 86 83 0.0 6 78 5 87 54 0.0 6 51 2 1 88 59 0.0 6 55 3 1 89 52 0.0 6 50 1 0 0 0 1 90 47 0.0 6 45 2 91 61 0.0 6 57 4 92 79 0.0 6 76 3 93 74 0.0 6 68 3 1 0 0 0 2 94 63 0.0 6 60 3 95 68 0.0 6 65 1 1 0 1 96 78 0.0 6 76 2 97 66 0.0 6 65 0 0 1 98 95 0.0 6 92 3 99 86 0.0 6 82 4 100 82 0.0 6 78 3 0 1 101 96 0.0 6 91 5 102 76 0.0 6 75 1 103 82 0.0 6 78 4 104 83 0.0 6 80 3 105 102 0.0 6 97 4 0 1 106 125 0.0 6 124 1 107 191 0.0 6 185 5 0 0 1 108 109 0.0 6 104 5 109 129 0.0 6 122 7 110 109 0.0 6 105 4 111 140 0.0 6 135 4 0 1 112 147 0.0 6 136 6 1 0 3 0 1 113 121 0.0 6 118 2 1 114 137 0.0 6 129 6 1 0 0 1 115 158 0.0 6 154 3 0 0 1 116 155 0.0 6 149 6 117 152 0.0 6 147 5 118 153 0.0 6 150 2 0 0 0 0 1 119 154 0.0 6 146 8 120 169 0.0 6 160 7 0 0 0 1 1 121 179 0.0 6 168 9 0 0 1 0 1 122 183 0.0 6 180 3 123 189 0.0 6 177 7 2 3 124 190 0.0 6 185 4 1 125 204 0.0 6 197 6 0 0 1 126 203 0.0 6 197 6 127 246 0.0 6 238 7 0 0 0 1 128 289 0.0 6 272 14 1 0 1 0 1 129 307 0.0 6 299 8 130 325 0.0 6 314 10 0 0 1 131 352 0.0 6 339 10 0 0 0 2 1 132 336 0.0 6 327 9 133 381 0.0 6 376 5 134 407 0.0 6 396 11 135 384 0.0 6 376 4 1 0 1 0 2 136 475 0.0 6 463 11 0 0 1 137 523 0.0 6 511 12 138 572 0.0 6 559 9 1 0 1 1 1 139 596 0.0 6 588 8 140 669 0.0 6 656 10 0 0 0 2 1 141 611 0.0 6 605 4 1 1 142 666 0.0 6 657 5 1 1 1 1 143 739 0.0 6 730 7 1 1 144 747 0.0 6 729 17 0 1 145 820 0.0 6 804 13 1 1 0 0 1 146 870 0.0 6 857 12 0 0 1 147 801 0.0 6 785 14 0 0 0 1 1 148 846 0.0 6 835 9 0 1 1 149 909 0.0 6 886 19 1 0 1 1 1 150 891 0.0 6 879 8 0 1 0 2 1 151 1163 0.0 6 1144 15 0 0 1 1 2 152 1238 0.0 6 1210 23 1 1 1 1 1 153 1490 0.0 6 1467 20 1 1 0 1 154 1176 0.0 6 1150 22 0 1 2 1 155 1496 0.0 6 1467 21 0 2 1 4 1 156 1375 0.0 6 1351 20 1 1 1 0 1 157 1127 0.0 6 1102 19 2 0 2 0 2 158 1246 0.0 6 1220 22 1 1 0 1 1 159 1028 0.0 6 1008 17 0 1 0 2 160 1139 0.0 6 1123 15 0 0 0 0 1 161 1078 0.0 6 1056 19 1 1 1 162 997 0.0 6 982 12 1 0 1 1 163 958 0.0 6 946 11 0 0 0 0 1 164 907 0.0 6 895 10 1 0 0 1 165 829 0.0 6 816 12 0 0 0 1 166 880 0.0 6 870 10 167 865 0.0 6 851 10 2 1 0 0 1 168 816 0.0 6 804 10 1 0 0 1 169 989 0.0 6 976 12 0 1 170 808 0.0 6 800 7 0 0 1 171 1027 0.0 6 1009 13 1 1 1 1 1 172 836 0.0 6 821 14 0 0 1 173 829 0.0 6 813 13 1 1 1 174 850 0.0 6 833 16 0 0 1 175 927 0.0 6 911 13 0 2 0 1 176 1067 0.0 6 1058 8 0 0 1 177 839 0.0 6 830 8 0 0 1 178 777 0.0 6 768 7 0 1 0 1 179 881 0.0 6 871 10 180 661 0.0 6 649 10 0 2 181 748 0.0 6 742 6 182 524 0.0 6 522 2 183 528 0.0 6 524 3 0 0 1 184 496 0.0 6 492 4 185 470 0.0 6 459 8 1 0 1 1 186 730 0.0 6 723 6 0 1 187 932 0.0 6 922 9 0 0 1 188 806 0.0 6 796 9 0 0 0 0 1 189 586 0.0 6 580 4 2 190 533 0.0 6 523 7 1 2 191 510 0.0 6 507 2 0 1 192 441 0.0 6 440 1 193 423 0.0 6 416 7 194 460 0.0 6 452 5 1 0 1 1 195 375 0.0 6 370 4 0 0 1 196 296 0.0 6 295 1 197 281 0.0 6 278 2 0 1 198 263 0.0 6 262 1 199 363 0.0 6 361 2 200 308 0.0 6 304 4 201 292 0.0 6 289 3 202 216 0.0 6 214 2 203 253 0.0 6 250 2 1 204 198 0.0 6 195 3 205 206 0.0 6 204 2 206 160 0.0 6 159 1 207 148 0.0 6 148 208 161 0.0 6 158 3 209 140 0.0 6 139 1 210 142 0.0 6 142 211 127 0.0 6 126 1 212 119 0.0 6 119 213 88 0.0 6 85 3 214 86 0.0 6 86 215 112 0.0 6 110 2 216 86 0.0 6 85 1 217 113 0.0 6 112 1 218 73 0.0 6 73 219 60 0.0 6 59 1 220 67 0.0 6 65 2 221 53 0.0 6 52 0 0 0 0 0 1 222 57 0.0 6 57 223 43 0.0 6 43 224 51 0.0 6 51 225 48 0.0 6 46 2 226 33 0.0 6 33 227 24 0.0 6 24 228 24 0.0 6 23 1 229 25 0.0 6 25 230 10 0.0 6 10 231 12 0.0 6 11 1 232 11 0.0 6 11 233 9 0.0 6 9 234 12 0.0 6 12 235 9 0.0 6 9 236 4 0.0 6 4 237 4 0.0 6 4 238 3 0.0 6 2 1 239 4 0.0 6 4 240 4 0.0 6 4 241 4 0.0 6 4 242 1 0.0 6 1 243 5 0.0 6 5 244 2 0.0 6 2 245 2 0.0 6 2 246 3 0.0 6 3 247 3 0.0 6 3 250 1 0.0 6 1 251 1 0.0 6 1 253 1 0.0 6 1 255 1 0.0 6 1 280 5 0.0 6 4 1 281 12 0.0 6 12 282 102 0.0 6 99 2 0 1 283 2 0.0 6 1 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 956 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 68 3 0.0 6 3 69 5 0.0 6 5 70 7 0.0 6 7 71 6 0.0 6 6 72 8 0.0 6 8 73 5 0.0 6 5 74 12 0.0 6 12 75 9 0.0 6 9 76 7 0.0 6 7 77 6 0.0 6 6 78 8 0.0 6 8 79 8 0.0 6 8 80 11 0.0 6 11 81 10 0.0 6 10 82 3 0.0 6 3 83 7 0.0 6 7 84 10 0.0 6 10 85 5 0.0 6 5 86 13 0.0 6 12 0 0 0 1 87 5 0.0 6 5 88 6 0.0 6 6 89 15 0.0 6 15 90 11 0.0 6 10 0 0 0 1 91 4 0.0 6 4 92 1 0.0 6 1 93 9 0.0 6 9 94 5 0.0 6 5 95 6 0.0 6 6 96 7 0.0 6 7 97 5 0.0 6 5 98 8 0.0 6 8 99 3 0.0 6 3 100 9 0.0 6 9 101 7 0.0 6 7 102 2 0.0 6 2 103 3 0.0 6 3 104 1 0.0 6 1 105 2 0.0 6 2 106 3 0.0 6 3 107 10 0.0 6 10 108 6 0.0 6 6 109 3 0.0 6 3 110 7 0.0 6 7 111 4 0.0 6 4 112 3 0.0 6 3 113 3 0.0 6 3 114 3 0.0 6 3 115 3 0.0 6 3 116 7 0.0 6 7 117 7 0.0 6 7 118 2 0.0 6 2 119 4 0.0 6 4 120 5 0.0 6 5 121 8 0.0 6 8 122 7 0.0 6 7 123 10 0.0 6 10 124 4 0.0 6 4 125 7 0.0 6 7 126 2 0.0 6 2 127 6 0.0 6 6 128 9 0.0 6 9 129 9 0.0 6 9 130 8 0.0 6 8 131 22 0.0 6 22 132 1 0.0 6 1 133 7 0.0 6 7 134 5 0.0 6 5 135 16 0.0 6 16 136 5 0.0 6 5 137 12 0.0 6 12 138 8 0.0 6 8 139 16 0.0 6 16 140 9 0.0 6 9 141 6 0.0 6 6 142 4 0.0 6 4 143 6 0.0 6 5 1 144 6 0.0 6 6 145 5 0.0 6 5 146 5 0.0 6 5 147 4 0.0 6 4 148 8 0.0 6 8 149 5 0.0 6 5 150 4 0.0 6 4 151 5 0.0 6 4 0 1 152 3 0.0 6 3 153 10 0.0 6 10 154 7 0.0 6 7 155 11 0.0 6 11 156 6 0.0 6 6 157 8 0.0 6 8 158 7 0.0 6 7 159 12 0.0 6 12 160 14 0.0 6 14 161 11 0.0 6 11 162 9 0.0 6 9 163 3 0.0 6 3 164 3 0.0 6 3 165 8 0.0 6 8 166 6 0.0 6 6 167 13 0.0 6 13 168 5 0.0 6 5 169 11 0.0 6 11 170 11 0.0 6 11 171 9 0.0 6 8 0 0 0 1 172 6 0.0 6 6 173 6 0.0 6 6 174 11 0.0 6 11 175 7 0.0 6 7 176 15 0.0 6 15 177 9 0.0 6 9 178 6 0.0 6 6 179 7 0.0 6 7 180 4 0.0 6 4 181 7 0.0 6 7 182 4 0.0 6 4 183 5 0.0 6 5 184 4 0.0 6 4 185 9 0.0 6 9 186 8 0.0 6 8 187 8 0.0 6 8 188 18 0.0 6 18 189 1 0.0 6 1 190 3 0.0 6 3 191 3 0.0 6 3 192 7 0.0 6 7 193 10 0.0 6 10 194 3 0.0 6 3 195 2 0.0 6 2 196 2 0.0 6 2 197 5 0.0 6 5 198 4 0.0 6 4 199 3 0.0 6 3 200 6 0.0 6 5 0 1 201 2 0.0 6 2 202 2 0.0 6 2 203 1 0.0 6 1 204 3 0.0 6 3 205 1 0.0 6 1 206 3 0.0 6 3 207 2 0.0 6 2 208 3 0.0 6 3 209 7 0.0 6 7 210 12 0.0 6 12 211 1 0.0 6 1 212 2 0.0 6 2 213 1 0.0 6 1 215 4 0.0 6 4 219 2 0.0 6 2 221 1 0.0 6 1 222 2 0.0 6 2 225 2 0.0 6 2 231 2 0.0 6 2 234 1 0.0 6 1 236 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_15_138_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_15_365_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_15_138_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_15_365_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.48 s (275 µs/read; 0.22 M reads/minute). === Summary === Total read pairs processed: 1,743 Read 1 with adapter: 1,743 (100.0%) Read 2 with adapter: 1,740 (99.8%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 3 (0.2%) Pairs written (passing filters): 1,740 (99.8%) Total basepairs processed: 606,306 bp Read 1: 308,612 bp Read 2: 297,694 bp Total written (filtered): 114,301 bp (18.9%) Read 1: 55,572 bp Read 2: 58,729 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 1693 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 1.7 1 1 20 5 0.0 6 5 21 4 0.0 6 4 22 1 0.0 6 1 26 1 0.0 6 1 41 1 0.0 6 1 43 1 0.0 6 1 47 1 0.0 6 1 51 1 0.0 6 1 52 1 0.0 6 1 66 1 0.0 6 1 71 1 0.0 6 1 73 1 0.0 6 1 75 1 0.0 6 1 76 1 0.0 6 1 77 1 0.0 6 1 78 1 0.0 6 1 79 1 0.0 6 1 80 2 0.0 6 2 81 1 0.0 6 1 83 1 0.0 6 1 87 1 0.0 6 1 89 1 0.0 6 1 97 1 0.0 6 1 104 3 0.0 6 3 106 3 0.0 6 3 107 1 0.0 6 0 1 108 2 0.0 6 2 109 1 0.0 6 1 110 3 0.0 6 3 111 4 0.0 6 4 112 1 0.0 6 1 114 1 0.0 6 1 115 5 0.0 6 5 117 1 0.0 6 1 118 1 0.0 6 1 119 1 0.0 6 1 120 1 0.0 6 1 122 1 0.0 6 1 123 1 0.0 6 1 124 1 0.0 6 1 125 4 0.0 6 4 126 6 0.0 6 6 127 1 0.0 6 1 128 2 0.0 6 2 129 1 0.0 6 1 131 1 0.0 6 1 132 5 0.0 6 5 133 1 0.0 6 1 134 2 0.0 6 2 135 2 0.0 6 2 136 1 0.0 6 1 137 1 0.0 6 1 138 6 0.0 6 6 139 1 0.0 6 1 140 9 0.0 6 9 141 16 0.0 6 16 142 23 0.0 6 23 143 327 0.0 6 326 1 144 224 0.0 6 224 145 194 0.0 6 194 146 29 0.0 6 29 147 21 0.0 6 21 148 295 0.0 6 295 149 126 0.0 6 126 150 94 0.0 6 94 151 17 0.0 6 17 152 16 0.0 6 16 153 9 0.0 6 9 154 16 0.0 6 16 156 12 0.0 6 12 157 7 0.0 6 6 1 158 2 0.0 6 2 159 55 0.0 6 55 160 18 0.0 6 18 161 1 0.0 6 1 162 2 0.0 6 2 163 1 0.0 6 1 164 7 0.0 6 7 165 1 0.0 6 1 169 1 0.0 6 1 171 2 0.0 6 2 173 2 0.0 6 2 174 3 0.0 6 3 175 1 0.0 6 1 178 1 0.0 6 1 185 2 0.0 6 2 186 3 0.0 6 3 190 1 0.0 6 1 192 1 0.0 6 1 197 1 0.0 6 1 199 2 0.0 6 2 200 5 0.0 6 5 202 2 0.0 6 2 203 1 0.0 6 1 205 1 0.0 6 1 206 1 0.0 6 1 208 1 0.0 6 1 209 1 0.0 6 1 211 1 0.0 6 1 212 1 0.0 6 1 213 1 0.0 6 1 214 2 0.0 6 2 215 2 0.0 6 2 216 1 0.0 6 1 217 1 0.0 6 1 218 2 0.0 6 2 220 3 0.0 6 3 221 5 0.0 6 5 227 1 0.0 6 1 228 1 0.0 6 1 234 1 0.0 6 1 240 1 0.0 6 1 245 1 0.0 6 1 246 1 0.0 6 1 247 1 0.0 6 1 248 1 0.0 6 1 249 1 0.0 6 1 255 2 0.0 6 2 257 1 0.0 6 1 261 1 0.0 6 1 267 1 0.0 6 1 277 1 0.0 6 1 278 1 0.0 6 1 279 2 0.0 6 2 280 1 0.0 6 1 281 4 0.0 6 4 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 50 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 6 0.0 6 6 70 8 0.0 6 8 71 11 0.0 6 11 72 5 0.0 6 5 75 10 0.0 6 9 1 76 3 0.0 6 3 77 1 0.0 6 1 78 2 0.0 6 2 79 1 0.0 6 1 85 2 0.0 6 2 89 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 1689 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 12 1 0.0 3 1 20 1 0.0 6 1 24 2 0.0 6 2 25 1 0.0 6 1 34 1 0.0 6 1 38 1 0.0 6 1 39 1 0.0 6 1 41 1 0.0 6 1 42 1 0.0 6 1 47 1 0.0 6 1 51 1 0.0 6 1 57 1 0.0 6 1 67 3 0.0 6 3 72 1 0.0 6 1 73 2 0.0 6 2 74 2 0.0 6 2 75 1 0.0 6 1 76 1 0.0 6 1 82 2 0.0 6 2 84 2 0.0 6 2 85 1 0.0 6 1 86 1 0.0 6 1 87 1 0.0 6 1 89 1 0.0 6 1 92 1 0.0 6 1 94 1 0.0 6 1 95 2 0.0 6 2 96 1 0.0 6 1 97 2 0.0 6 2 98 1 0.0 6 1 100 5 0.0 6 5 102 3 0.0 6 3 103 2 0.0 6 2 104 3 0.0 6 3 105 2 0.0 6 2 106 2 0.0 6 2 107 3 0.0 6 3 108 1 0.0 6 1 109 2 0.0 6 2 110 5 0.0 6 5 111 8 0.0 6 8 112 5 0.0 6 5 113 3 0.0 6 3 114 5 0.0 6 5 115 3 0.0 6 3 116 4 0.0 6 4 117 9 0.0 6 9 118 4 0.0 6 4 119 6 0.0 6 6 120 2 0.0 6 2 121 15 0.0 6 15 122 4 0.0 6 4 123 7 0.0 6 7 124 6 0.0 6 6 125 6 0.0 6 6 126 15 0.0 6 15 127 5 0.0 6 5 128 7 0.0 6 6 1 129 3 0.0 6 3 130 4 0.0 6 4 131 7 0.0 6 7 132 6 0.0 6 6 133 19 0.0 6 19 134 28 0.0 6 28 135 33 0.0 6 33 136 36 0.0 6 36 137 68 0.0 6 68 138 285 0.0 6 284 1 139 160 0.0 6 160 140 147 0.0 6 147 141 45 0.0 6 45 142 52 0.0 6 52 143 223 0.0 6 223 144 113 0.0 6 113 145 63 0.0 6 63 146 21 0.0 6 21 147 11 0.0 6 11 148 11 0.0 6 11 149 13 0.0 6 13 150 10 0.0 6 9 1 151 18 0.0 6 18 152 8 0.0 6 8 153 14 0.0 6 14 154 36 0.0 6 35 1 155 7 0.0 6 7 157 4 0.0 6 4 158 3 0.0 6 3 159 4 0.0 6 4 160 2 0.0 6 2 162 3 0.0 6 3 163 2 0.0 6 2 164 1 0.0 6 1 166 1 0.0 6 1 167 2 0.0 6 2 168 1 0.0 6 1 169 2 0.0 6 2 170 2 0.0 6 2 173 1 0.0 6 1 177 2 0.0 6 2 178 1 0.0 6 1 179 1 0.0 6 1 182 1 0.0 6 1 183 2 0.0 6 2 184 3 0.0 6 3 185 1 0.0 6 1 187 1 0.0 6 1 188 1 0.0 6 1 189 3 0.0 6 3 192 1 0.0 6 1 194 2 0.0 6 1 1 196 1 0.0 6 1 197 3 0.0 6 3 199 1 0.0 6 1 203 2 0.0 6 2 204 2 0.0 6 2 206 1 0.0 6 1 211 1 0.0 6 1 213 2 0.0 6 2 214 1 0.0 6 1 215 2 0.0 6 2 218 1 0.0 6 1 224 1 0.0 6 1 229 1 0.0 6 1 243 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 51 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 2 0.0 6 2 72 15 0.0 6 14 1 73 4 0.0 6 4 74 3 0.0 6 3 76 7 0.0 6 7 77 10 0.0 6 10 78 3 0.0 6 3 87 1 0.0 6 1 88 2 0.0 6 2 129 1 0.0 6 1 144 1 0.0 6 1 150 1 0.0 6 1 177 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_16_139_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_16_366_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_16_139_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_16_366_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.89 s (28 µs/read; 2.12 M reads/minute). === Summary === Total read pairs processed: 102,017 Read 1 with adapter: 102,005 (100.0%) Read 2 with adapter: 101,975 (100.0%) == Read fate breakdown == Pairs that were too short: 54 (0.1%) Pairs discarded as untrimmed: 54 (0.1%) Pairs written (passing filters): 101,909 (99.9%) Total basepairs processed: 42,998,595 bp Read 1: 23,246,708 bp Read 2: 19,751,887 bp Total written (filtered): 6,459,753 bp (15.0%) Read 1: 3,250,304 bp Read 2: 3,209,449 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 98234 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 1594.0 0 2 1 4 4 398.5 1 1 3 5 3 99.6 1 3 6 2 24.9 1 1 0 1 7 3 6.2 2 3 9 4 0.4 2 4 10 1 0.1 3 1 11 1 0.0 3 0 1 15 1 0.0 4 1 16 5 0.0 4 5 17 4 0.0 5 3 1 18 2 0.0 5 2 19 1 0.0 5 1 20 158 0.0 6 151 7 21 89 0.0 6 85 4 22 81 0.0 6 80 0 1 23 64 0.0 6 59 5 24 43 0.0 6 42 0 0 0 1 25 33 0.0 6 33 26 34 0.0 6 28 4 2 27 24 0.0 6 22 2 28 24 0.0 6 23 1 29 24 0.0 6 22 2 30 7 0.0 6 6 1 31 13 0.0 6 11 2 32 11 0.0 6 11 33 11 0.0 6 10 0 0 0 0 1 34 10 0.0 6 8 2 35 7 0.0 6 6 0 1 36 11 0.0 6 11 37 13 0.0 6 13 38 6 0.0 6 5 1 39 11 0.0 6 11 40 10 0.0 6 8 1 1 41 15 0.0 6 15 42 7 0.0 6 6 1 43 7 0.0 6 6 1 44 8 0.0 6 7 0 1 45 1 0.0 6 1 46 6 0.0 6 6 47 2 0.0 6 2 48 6 0.0 6 6 49 5 0.0 6 5 50 5 0.0 6 5 51 8 0.0 6 8 52 11 0.0 6 10 1 53 12 0.0 6 12 54 10 0.0 6 10 55 5 0.0 6 5 56 5 0.0 6 5 58 5 0.0 6 4 1 59 9 0.0 6 8 0 0 1 60 6 0.0 6 6 61 5 0.0 6 5 62 7 0.0 6 6 1 63 10 0.0 6 10 64 4 0.0 6 4 65 14 0.0 6 14 66 7 0.0 6 6 1 67 8 0.0 6 8 68 6 0.0 6 5 1 69 13 0.0 6 12 1 70 8 0.0 6 7 0 0 0 0 1 71 12 0.0 6 11 0 0 0 0 0 1 72 18 0.0 6 17 0 0 1 73 16 0.0 6 16 74 11 0.0 6 11 75 13 0.0 6 13 76 9 0.0 6 9 77 14 0.0 6 14 78 10 0.0 6 9 0 1 79 10 0.0 6 10 80 3 0.0 6 3 81 8 0.0 6 8 82 8 0.0 6 8 83 14 0.0 6 13 0 1 84 10 0.0 6 10 85 8 0.0 6 8 86 19 0.0 6 18 1 87 6 0.0 6 6 88 14 0.0 6 12 0 0 2 89 8 0.0 6 7 1 90 5 0.0 6 5 91 9 0.0 6 9 92 6 0.0 6 6 93 4 0.0 6 4 94 11 0.0 6 11 95 8 0.0 6 8 96 5 0.0 6 5 97 6 0.0 6 6 98 7 0.0 6 7 99 16 0.0 6 15 1 100 9 0.0 6 9 101 13 0.0 6 13 102 18 0.0 6 17 1 103 15 0.0 6 13 2 104 16 0.0 6 16 105 24 0.0 6 23 1 106 20 0.0 6 20 107 31 0.0 6 30 0 0 1 108 15 0.0 6 15 109 25 0.0 6 25 110 17 0.0 6 17 111 13 0.0 6 13 112 22 0.0 6 22 113 11 0.0 6 11 114 25 0.0 6 24 1 115 20 0.0 6 20 116 18 0.0 6 16 1 0 0 0 1 117 26 0.0 6 26 118 40 0.0 6 38 1 0 0 0 1 119 36 0.0 6 34 1 1 120 53 0.0 6 52 0 0 0 1 121 52 0.0 6 51 1 122 47 0.0 6 47 123 56 0.0 6 55 1 124 46 0.0 6 46 125 51 0.0 6 46 2 1 2 126 82 0.0 6 75 3 2 2 127 149 0.0 6 146 2 1 128 81 0.0 6 76 3 2 129 105 0.0 6 102 0 1 2 130 117 0.0 6 114 2 0 1 131 136 0.0 6 131 3 0 2 132 114 0.0 6 111 2 0 1 133 72 0.0 6 70 0 1 0 0 0 1 134 76 0.0 6 74 1 0 1 135 79 0.0 6 79 136 89 0.0 6 88 1 137 63 0.0 6 61 1 0 1 138 56 0.0 6 54 1 1 139 59 0.0 6 59 140 80 0.0 6 79 1 141 83 0.0 6 82 1 142 160 0.0 6 158 2 143 808 0.0 6 805 3 144 518 0.0 6 517 1 145 537 0.0 6 533 2 0 1 1 146 236 0.0 6 235 0 0 1 147 1141 0.0 6 1137 2 1 1 148 903 0.0 6 902 0 0 1 149 164 0.0 6 160 4 150 133 0.0 6 132 0 0 1 151 175 0.0 6 174 0 0 1 152 220 0.0 6 217 3 153 194 0.0 6 191 3 154 301 0.0 6 297 1 1 2 155 2059 0.0 6 2049 6 1 1 0 2 156 1763 0.0 6 1760 1 2 157 1681 0.0 6 1676 4 1 158 527 0.0 6 526 1 159 280 0.0 6 279 1 160 261 0.0 6 259 1 1 161 414 0.0 6 412 2 162 2291 0.0 6 2286 3 0 1 0 1 163 2074 0.0 6 2066 7 1 164 2145 0.0 6 2143 1 1 165 624 0.0 6 622 2 166 1990 0.0 6 1985 5 167 600 0.0 6 597 3 168 2465 0.0 6 2461 3 0 1 169 2446 0.0 6 2441 5 170 339 0.0 6 337 1 1 171 242 0.0 6 240 1 0 0 0 1 172 340 0.0 6 337 1 1 0 0 1 173 1928 0.0 6 1920 6 0 0 0 2 174 393 0.0 6 391 2 175 285 0.0 6 284 1 176 287 0.0 6 285 1 0 0 1 177 604 0.0 6 603 1 178 3202 0.0 6 3192 8 0 1 0 1 179 712 0.0 6 711 1 180 341 0.0 6 337 3 0 1 181 1894 0.0 6 1888 5 0 1 182 621 0.0 6 617 4 183 275 0.0 6 271 3 1 184 427 0.0 6 424 1 1 1 185 1849 0.0 6 1847 1 0 1 186 894 0.0 6 891 1 0 1 0 0 1 187 241 0.0 6 240 1 188 325 0.0 6 323 1 0 0 1 189 305 0.0 6 303 1 1 190 251 0.0 6 250 1 191 411 0.0 6 407 2 1 0 1 192 2344 0.0 6 2336 7 0 1 193 455 0.0 6 453 0 0 1 0 0 1 194 291 0.0 6 285 6 195 239 0.0 6 233 4 0 0 1 0 1 196 340 0.0 6 329 7 2 1 1 197 279 0.0 6 277 1 0 0 0 0 1 198 399 0.0 6 390 7 1 1 199 570 0.0 6 565 4 0 1 200 1028 0.0 6 1023 2 0 3 201 488 0.0 6 482 4 1 0 0 0 1 202 566 0.0 6 557 7 1 1 203 441 0.0 6 434 5 0 1 1 204 518 0.0 6 508 8 2 205 870 0.0 6 858 8 1 2 1 206 976 0.0 6 964 12 207 968 0.0 6 954 6 4 1 3 208 3913 0.0 6 3905 7 0 0 1 209 466 0.0 6 461 5 210 446 0.0 6 438 4 1 1 1 0 1 211 662 0.0 6 654 7 0 0 1 212 961 0.0 6 954 7 213 464 0.0 6 455 7 0 0 1 0 1 214 674 0.0 6 662 8 1 3 215 737 0.0 6 730 5 1 1 216 749 0.0 6 742 3 3 0 0 1 217 1045 0.0 6 1037 3 3 2 218 507 0.0 6 500 4 2 0 0 0 1 219 562 0.0 6 549 6 3 2 1 0 1 220 638 0.0 6 626 9 0 2 0 0 1 221 542 0.0 6 535 4 2 0 0 1 222 648 0.0 6 644 2 2 223 637 0.0 6 625 8 2 2 224 294 0.0 6 290 3 1 225 334 0.0 6 331 3 226 359 0.0 6 357 2 227 628 0.0 6 622 5 0 1 228 534 0.0 6 523 7 1 3 229 502 0.0 6 497 4 0 0 1 230 400 0.0 6 394 5 0 1 231 491 0.0 6 484 4 2 1 232 531 0.0 6 525 5 0 1 233 587 0.0 6 580 6 1 234 527 0.0 6 515 6 2 2 0 0 2 235 593 0.0 6 590 2 1 236 370 0.0 6 366 2 1 0 0 0 1 237 376 0.0 6 371 2 3 238 541 0.0 6 538 2 0 1 239 844 0.0 6 843 0 0 1 240 1245 0.0 6 1237 7 0 1 241 2017 0.0 6 2013 3 0 1 242 360 0.0 6 358 2 243 453 0.0 6 446 5 1 1 244 483 0.0 6 474 5 3 1 245 691 0.0 6 687 3 0 1 246 335 0.0 6 330 2 2 1 247 426 0.0 6 421 3 1 1 248 140 0.0 6 138 1 0 1 249 177 0.0 6 173 3 0 1 250 268 0.0 6 263 3 1 1 251 305 0.0 6 301 4 252 321 0.0 6 319 2 253 482 0.0 6 477 3 2 254 333 0.0 6 322 8 2 1 255 385 0.0 6 383 2 256 255 0.0 6 253 1 1 257 285 0.0 6 285 258 319 0.0 6 313 4 0 0 0 0 2 259 433 0.0 6 431 1 0 1 260 336 0.0 6 333 2 0 0 0 1 261 356 0.0 6 352 4 262 444 0.0 6 437 5 1 1 263 212 0.0 6 210 1 1 264 285 0.0 6 283 2 265 348 0.0 6 338 7 0 2 0 1 266 277 0.0 6 273 4 267 316 0.0 6 316 268 500 0.0 6 500 269 436 0.0 6 434 1 1 270 474 0.0 6 471 1 1 1 271 340 0.0 6 338 1 1 272 237 0.0 6 236 1 273 212 0.0 6 208 3 1 274 271 0.0 6 268 1 1 1 275 187 0.0 6 183 3 1 276 295 0.0 6 292 1 0 2 277 315 0.0 6 314 0 0 1 278 288 0.0 6 286 2 279 322 0.0 6 318 3 1 280 2789 0.0 6 2761 19 5 3 0 1 281 282 0.0 6 279 2 0 1 282 8 0.0 6 8 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 3771 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 18 0.0 6 18 72 46 0.0 6 46 73 22 0.0 6 22 74 29 0.0 6 29 75 14 0.0 6 14 76 59 0.0 6 59 77 37 0.0 6 36 1 78 1 0.0 6 1 81 2 0.0 6 2 82 2 0.0 6 2 83 25 0.0 6 25 84 99 0.0 6 99 85 80 0.0 6 80 86 84 0.0 6 83 1 87 9 0.0 6 9 88 1 0.0 6 1 89 3 0.0 6 3 90 35 0.0 6 35 91 142 0.0 6 141 0 1 92 88 0.0 6 87 1 93 91 0.0 6 91 94 36 0.0 6 36 95 86 0.0 6 86 96 33 0.0 6 33 97 106 0.0 6 106 98 118 0.0 6 118 99 3 0.0 6 3 100 1 0.0 6 1 101 24 0.0 6 24 102 81 0.0 6 81 103 2 0.0 6 2 105 5 0.0 6 5 106 51 0.0 6 51 107 177 0.0 6 177 108 20 0.0 6 20 109 22 0.0 6 22 110 83 0.0 6 83 111 14 0.0 6 14 112 7 0.0 6 6 1 113 32 0.0 6 32 114 82 0.0 6 82 115 25 0.0 6 25 116 5 0.0 6 5 117 4 0.0 6 4 118 1 0.0 6 1 119 1 0.0 6 1 120 34 0.0 6 34 121 114 0.0 6 114 122 3 0.0 6 3 126 2 0.0 6 2 127 4 0.0 6 4 128 9 0.0 6 9 129 22 0.0 6 22 130 2 0.0 6 2 131 1 0.0 6 1 133 1 0.0 6 1 134 7 0.0 6 7 135 29 0.0 6 29 136 65 0.0 6 65 137 202 0.0 6 201 1 138 5 0.0 6 5 140 4 0.0 6 4 141 19 0.0 6 19 142 2 0.0 6 2 149 1 0.0 6 1 150 4 0.0 6 4 151 11 0.0 6 11 153 1 0.0 6 1 155 4 0.0 6 4 156 18 0.0 6 18 157 1 0.0 6 1 159 1 0.0 6 1 162 1 0.0 6 1 163 4 0.0 6 4 167 1 0.0 6 1 172 1 0.0 6 1 173 38 0.0 6 38 174 110 0.0 6 109 1 175 20 0.0 6 20 176 1 0.0 6 1 181 2 0.0 6 2 182 5 0.0 6 5 183 18 0.0 6 17 1 184 104 0.0 6 104 185 124 0.0 6 124 186 23 0.0 6 22 1 187 1 0.0 6 1 192 3 0.0 6 3 193 1 0.0 6 1 198 1 0.0 6 1 200 4 0.0 6 4 201 15 0.0 6 14 1 202 54 0.0 6 54 203 201 0.0 6 201 204 6 0.0 6 6 205 1 0.0 6 1 213 1 0.0 6 1 214 2 0.0 6 2 222 1 0.0 6 1 229 25 0.0 6 25 230 80 0.0 6 79 1 231 4 0.0 6 3 1 235 1 0.0 6 1 240 1 0.0 6 1 242 5 0.0 6 5 243 44 0.0 6 44 244 102 0.0 6 102 245 4 0.0 6 4 250 16 0.0 6 16 251 33 0.0 6 33 252 4 0.0 6 4 253 52 0.0 6 52 254 121 0.0 6 121 255 9 0.0 6 9 256 14 0.0 6 14 259 1 0.0 6 1 260 1 0.0 6 1 279 2 0.0 6 2 282 3 0.0 6 3 288 1 0.0 6 1 290 2 0.0 6 2 294 1 0.0 6 1 297 2 0.0 6 1 1 298 11 0.0 6 11 299 14 0.0 6 14 300 1 0.0 6 1 303 1 0.0 6 1 304 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 98475 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 2 24.9 1 2 8 1 1.6 2 1 11 3 0.0 3 3 12 4 0.0 3 4 13 13 0.0 3 10 2 0 1 14 7 0.0 4 6 1 16 16 0.0 4 13 3 17 10 0.0 5 10 18 15 0.0 5 12 3 19 18 0.0 5 17 1 20 125 0.0 6 118 7 21 69 0.0 6 65 4 22 42 0.0 6 40 2 23 28 0.0 6 28 24 24 0.0 6 23 1 25 20 0.0 6 17 3 26 17 0.0 6 16 1 27 15 0.0 6 15 28 20 0.0 6 19 0 1 29 18 0.0 6 16 2 30 26 0.0 6 25 1 31 25 0.0 6 25 32 12 0.0 6 11 1 33 17 0.0 6 15 2 34 6 0.0 6 5 1 35 11 0.0 6 9 2 36 16 0.0 6 13 3 37 10 0.0 6 10 38 12 0.0 6 10 0 0 1 1 39 14 0.0 6 14 40 12 0.0 6 12 41 12 0.0 6 10 1 0 0 0 0 1 42 15 0.0 6 14 1 43 28 0.0 6 27 1 44 13 0.0 6 12 1 45 34 0.0 6 31 1 0 0 0 0 2 46 47 0.0 6 44 2 0 0 0 0 1 47 43 0.0 6 42 1 48 25 0.0 6 25 49 18 0.0 6 17 0 1 50 22 0.0 6 22 51 28 0.0 6 26 1 0 0 0 1 52 12 0.0 6 12 53 31 0.0 6 30 1 54 25 0.0 6 23 1 0 1 55 26 0.0 6 24 1 0 0 1 56 21 0.0 6 19 1 0 0 0 1 57 26 0.0 6 24 2 58 26 0.0 6 23 2 0 0 0 0 1 59 28 0.0 6 24 3 0 1 60 24 0.0 6 21 3 61 24 0.0 6 21 3 62 21 0.0 6 21 63 19 0.0 6 18 1 64 22 0.0 6 20 2 65 40 0.0 6 37 3 66 46 0.0 6 42 2 1 0 0 1 67 32 0.0 6 31 1 68 63 0.0 6 59 4 69 178 0.0 6 170 5 2 1 70 68 0.0 6 66 0 0 0 1 1 71 89 0.0 6 82 4 1 1 0 1 72 37 0.0 6 34 3 73 85 0.0 6 81 3 0 1 74 41 0.0 6 39 1 0 0 1 75 39 0.0 6 37 1 1 76 36 0.0 6 36 77 54 0.0 6 51 3 78 29 0.0 6 25 3 0 0 1 79 36 0.0 6 33 1 0 0 1 0 1 80 39 0.0 6 38 0 1 81 42 0.0 6 41 1 82 51 0.0 6 48 3 83 48 0.0 6 47 0 0 0 0 0 1 84 67 0.0 6 65 1 0 0 0 0 1 85 81 0.0 6 78 3 86 46 0.0 6 43 1 0 0 1 0 1 87 47 0.0 6 43 3 0 0 1 88 44 0.0 6 43 1 89 56 0.0 6 55 1 90 43 0.0 6 41 2 91 50 0.0 6 49 1 92 62 0.0 6 59 2 0 1 93 63 0.0 6 59 3 0 0 1 94 60 0.0 6 59 1 95 64 0.0 6 58 6 96 80 0.0 6 78 0 2 97 54 0.0 6 51 1 0 1 1 98 73 0.0 6 69 3 0 0 0 0 1 99 60 0.0 6 58 2 100 45 0.0 6 44 1 101 68 0.0 6 67 1 102 53 0.0 6 46 5 0 0 0 1 1 103 42 0.0 6 40 2 104 67 0.0 6 60 5 0 1 0 0 1 105 97 0.0 6 96 1 106 123 0.0 6 119 4 107 86 0.0 6 83 3 108 114 0.0 6 107 5 1 0 1 109 134 0.0 6 131 1 1 0 1 110 100 0.0 6 93 7 111 86 0.0 6 85 1 112 126 0.0 6 123 2 0 0 1 113 107 0.0 6 106 1 114 140 0.0 6 137 3 115 134 0.0 6 128 4 2 116 129 0.0 6 125 4 117 170 0.0 6 164 4 0 0 1 1 118 125 0.0 6 119 6 119 160 0.0 6 155 4 0 0 0 0 1 120 169 0.0 6 166 1 0 0 1 0 1 121 169 0.0 6 165 2 2 122 173 0.0 6 170 1 0 0 1 1 123 131 0.0 6 129 2 124 128 0.0 6 125 2 1 125 135 0.0 6 134 1 126 200 0.0 6 198 2 127 235 0.0 6 227 7 0 0 0 1 128 243 0.0 6 238 4 0 0 1 129 285 0.0 6 277 8 130 237 0.0 6 232 5 131 326 0.0 6 319 5 1 0 1 132 317 0.0 6 309 8 133 390 0.0 6 384 4 2 134 335 0.0 6 332 3 135 587 0.0 6 566 12 2 1 3 3 136 615 0.0 6 602 9 0 0 4 137 650 0.0 6 644 3 1 0 1 1 138 602 0.0 6 596 5 0 1 139 735 0.0 6 719 10 0 0 1 3 2 140 976 0.0 6 967 4 1 0 2 1 1 141 926 0.0 6 910 10 0 0 2 3 1 142 993 0.0 6 980 9 0 2 0 1 1 143 960 0.0 6 942 14 0 2 0 1 1 144 1868 0.0 6 1851 13 0 0 2 2 145 1181 0.0 6 1169 8 0 0 3 0 1 146 870 0.0 6 854 11 0 0 2 2 1 147 1019 0.0 6 1009 9 0 0 0 1 148 1072 0.0 6 1051 15 1 0 3 1 1 149 837 0.0 6 822 13 0 0 1 1 150 1121 0.0 6 1095 16 6 0 2 1 1 151 1711 0.0 6 1675 27 1 0 1 5 2 152 3311 0.0 6 3262 36 4 0 7 2 153 2515 0.0 6 2488 19 3 1 1 2 1 154 2317 0.0 6 2283 26 1 1 3 1 2 155 1792 0.0 6 1762 20 2 0 3 4 1 156 1443 0.0 6 1421 17 2 0 3 157 1983 0.0 6 1954 19 1 1 3 4 1 158 2023 0.0 6 1996 19 2 0 4 2 159 2594 0.0 6 2569 18 2 1 2 2 160 2390 0.0 6 2354 27 2 0 4 0 3 161 2049 0.0 6 2031 16 2 162 1750 0.0 6 1721 25 1 0 1 1 1 163 1372 0.0 6 1363 9 164 1228 0.0 6 1214 12 0 0 2 165 1909 0.0 6 1893 13 1 0 1 0 1 166 1682 0.0 6 1661 15 3 1 1 0 1 167 835 0.0 6 814 18 2 0 0 0 1 168 1304 0.0 6 1269 30 3 1 0 0 1 169 976 0.0 6 955 17 1 1 1 1 170 1802 0.0 6 1776 20 3 0 1 1 1 171 1047 0.0 6 1022 21 1 0 2 1 172 1331 0.0 6 1300 27 1 0 2 1 173 1144 0.0 6 1138 5 0 0 1 174 1491 0.0 6 1478 10 1 0 2 175 2252 0.0 6 2239 10 0 0 1 2 176 1371 0.0 6 1355 14 1 0 1 177 983 0.0 6 975 5 0 1 1 1 178 1648 0.0 6 1633 8 3 0 2 2 179 869 0.0 6 863 6 180 836 0.0 6 823 11 0 0 0 2 181 727 0.0 6 714 13 182 750 0.0 6 747 3 183 640 0.0 6 635 5 184 568 0.0 6 565 2 0 0 0 1 185 922 0.0 6 901 14 4 0 0 2 1 186 1371 0.0 6 1351 17 0 0 1 2 187 1230 0.0 6 1219 9 0 0 0 1 1 188 1154 0.0 6 1148 6 189 1230 0.0 6 1212 16 2 190 689 0.0 6 682 7 191 598 0.0 6 588 10 192 533 0.0 6 527 5 0 0 0 0 1 193 697 0.0 6 691 6 194 575 0.0 6 560 13 1 0 0 0 1 195 593 0.0 6 583 9 0 0 0 1 196 428 0.0 6 416 10 0 0 0 0 2 197 453 0.0 6 445 8 198 673 0.0 6 658 12 2 0 0 0 1 199 615 0.0 6 607 7 0 0 0 1 200 539 0.0 6 538 1 201 694 0.0 6 689 4 1 202 622 0.0 6 618 4 203 549 0.0 6 545 3 0 0 0 1 204 546 0.0 6 545 1 205 843 0.0 6 840 3 206 271 0.0 6 265 5 1 207 195 0.0 6 193 1 1 208 357 0.0 6 352 4 1 209 299 0.0 6 292 6 0 0 1 210 285 0.0 6 279 5 1 211 429 0.0 6 425 3 1 212 379 0.0 6 369 8 2 213 493 0.0 6 480 12 0 0 0 0 1 214 205 0.0 6 201 4 215 304 0.0 6 298 6 216 429 0.0 6 421 7 0 0 0 1 217 250 0.0 6 246 3 1 218 237 0.0 6 222 12 2 0 0 1 219 546 0.0 6 532 10 4 220 251 0.0 6 244 5 1 0 0 1 221 132 0.0 6 128 4 222 187 0.0 6 181 5 1 223 271 0.0 6 267 3 1 224 146 0.0 6 145 1 225 81 0.0 6 80 1 226 67 0.0 6 66 1 227 59 0.0 6 58 1 228 46 0.0 6 46 229 41 0.0 6 40 1 230 42 0.0 6 42 231 42 0.0 6 42 232 28 0.0 6 28 233 44 0.0 6 44 234 43 0.0 6 42 1 235 19 0.0 6 19 236 15 0.0 6 15 237 10 0.0 6 10 238 14 0.0 6 14 239 12 0.0 6 12 240 26 0.0 6 26 241 24 0.0 6 24 242 7 0.0 6 7 243 20 0.0 6 19 0 1 244 19 0.0 6 19 245 11 0.0 6 11 246 5 0.0 6 5 247 13 0.0 6 13 248 20 0.0 6 19 1 249 10 0.0 6 9 1 250 13 0.0 6 13 251 4 0.0 6 4 252 3 0.0 6 3 253 25 0.0 6 25 254 3 0.0 6 3 256 2 0.0 6 2 263 1 0.0 6 1 279 1 0.0 6 1 280 9 0.0 6 8 1 281 21 0.0 6 20 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 3500 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 47 2 0.0 6 0 2 50 1 0.0 6 1 51 3 0.0 6 3 53 1 0.0 6 1 56 1 0.0 6 1 62 1 0.0 6 0 0 0 0 1 63 2 0.0 6 1 0 0 1 66 2 0.0 6 2 70 2 0.0 6 2 71 8 0.0 6 8 72 49 0.0 6 49 73 23 0.0 6 23 74 27 0.0 6 27 75 11 0.0 6 11 76 69 0.0 6 69 77 53 0.0 6 53 78 1 0.0 6 1 80 2 0.0 6 2 81 3 0.0 6 3 82 1 0.0 6 1 83 18 0.0 6 18 84 128 0.0 6 128 85 84 0.0 6 84 86 84 0.0 6 84 87 14 0.0 6 13 0 0 0 1 88 5 0.0 6 2 0 1 1 1 89 6 0.0 6 6 90 25 0.0 6 25 91 136 0.0 6 136 92 110 0.0 6 110 93 101 0.0 6 100 1 94 27 0.0 6 26 1 95 107 0.0 6 105 0 0 0 2 96 21 0.0 6 20 0 0 0 1 97 163 0.0 6 160 1 0 0 2 98 133 0.0 6 133 99 2 0.0 6 2 100 1 0.0 6 0 0 0 1 101 21 0.0 6 21 102 103 0.0 6 103 103 3 0.0 6 3 105 2 0.0 6 1 0 0 1 106 37 0.0 6 35 0 0 0 2 107 208 0.0 6 208 108 29 0.0 6 28 1 109 18 0.0 6 18 110 141 0.0 6 140 1 111 13 0.0 6 13 112 3 0.0 6 3 113 17 0.0 6 16 0 0 1 114 111 0.0 6 109 0 0 0 2 115 31 0.0 6 31 116 1 0.0 6 0 0 0 0 1 117 3 0.0 6 3 118 4 0.0 6 4 119 1 0.0 6 1 120 29 0.0 6 29 121 145 0.0 6 145 122 3 0.0 6 3 124 1 0.0 6 1 126 3 0.0 6 3 127 2 0.0 6 2 128 4 0.0 6 4 129 30 0.0 6 30 130 1 0.0 6 1 133 5 0.0 6 3 1 0 0 1 134 5 0.0 6 5 135 43 0.0 6 42 1 136 31 0.0 6 30 1 137 227 0.0 6 227 138 3 0.0 6 3 139 1 0.0 6 1 140 3 0.0 6 3 141 15 0.0 6 12 0 0 0 3 144 1 0.0 6 1 147 1 0.0 6 1 148 1 0.0 6 1 149 1 0.0 6 1 150 3 0.0 6 3 151 10 0.0 6 10 155 5 0.0 6 5 156 27 0.0 6 27 162 1 0.0 6 1 163 7 0.0 6 7 167 1 0.0 6 1 171 1 0.0 6 1 172 2 0.0 6 2 173 23 0.0 6 23 174 111 0.0 6 111 175 17 0.0 6 16 1 178 1 0.0 6 0 0 0 0 1 181 1 0.0 6 0 1 182 3 0.0 6 3 183 17 0.0 6 17 184 92 0.0 6 92 185 178 0.0 6 178 186 22 0.0 6 20 1 0 1 187 1 0.0 6 1 189 1 0.0 6 0 0 0 0 1 192 3 0.0 6 3 200 4 0.0 6 4 201 9 0.0 6 9 202 33 0.0 6 33 203 147 0.0 6 147 204 5 0.0 6 4 1 207 2 0.0 6 0 0 0 0 2 210 1 0.0 6 1 214 1 0.0 6 1 215 1 0.0 6 1 217 1 0.0 6 0 0 1 229 3 0.0 6 3 230 11 0.0 6 11 250 1 0.0 6 1 251 11 0.0 6 11 253 2 0.0 6 2 254 12 0.0 6 12 256 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_17_140_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_17_367_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_17_140_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_17_367_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.16 s (32 µs/read; 1.86 M reads/minute). === Summary === Total read pairs processed: 67,163 Read 1 with adapter: 67,160 (100.0%) Read 2 with adapter: 67,142 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 24 (0.0%) Pairs written (passing filters): 67,139 (100.0%) Total basepairs processed: 34,799,919 bp Read 1: 19,534,343 bp Read 2: 15,265,576 bp Total written (filtered): 3,961,252 bp (11.4%) Read 1: 2,014,187 bp Read 2: 1,947,065 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 67158 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1049.4 0 0 1 4 1 262.4 1 0 1 8 1 1.0 2 1 9 3 0.3 2 3 10 1 0.1 3 1 16 2 0.0 4 1 1 18 1 0.0 5 0 1 20 9 0.0 6 9 21 7 0.0 6 7 22 6 0.0 6 6 23 4 0.0 6 4 24 3 0.0 6 3 25 2 0.0 6 2 27 1 0.0 6 1 28 3 0.0 6 3 29 2 0.0 6 2 32 1 0.0 6 1 33 2 0.0 6 1 0 0 0 0 1 36 1 0.0 6 1 37 2 0.0 6 2 39 1 0.0 6 1 40 2 0.0 6 2 41 3 0.0 6 3 44 3 0.0 6 3 45 23 0.0 6 23 46 8 0.0 6 8 47 2 0.0 6 2 48 1 0.0 6 1 49 1 0.0 6 0 0 0 0 1 50 1 0.0 6 1 51 2 0.0 6 1 1 53 1 0.0 6 1 54 3 0.0 6 3 55 4 0.0 6 4 57 2 0.0 6 2 58 3 0.0 6 3 60 2 0.0 6 2 63 1 0.0 6 1 65 1 0.0 6 1 66 1 0.0 6 1 68 3 0.0 6 3 69 1 0.0 6 1 70 1 0.0 6 1 71 1 0.0 6 1 73 2 0.0 6 2 74 2 0.0 6 2 75 1 0.0 6 1 76 3 0.0 6 3 77 2 0.0 6 2 78 1 0.0 6 1 79 2 0.0 6 2 80 2 0.0 6 2 85 2 0.0 6 2 87 7 0.0 6 7 88 2 0.0 6 1 1 89 1 0.0 6 1 90 4 0.0 6 4 91 1 0.0 6 1 93 2 0.0 6 2 94 3 0.0 6 3 95 1 0.0 6 1 97 2 0.0 6 2 98 3 0.0 6 3 99 1 0.0 6 1 100 1 0.0 6 0 1 101 2 0.0 6 2 102 10 0.0 6 8 2 103 3 0.0 6 3 104 1 0.0 6 1 105 6 0.0 6 6 106 4 0.0 6 3 1 107 8 0.0 6 8 108 9 0.0 6 9 109 6 0.0 6 5 1 111 6 0.0 6 6 112 3 0.0 6 3 113 1 0.0 6 1 114 2 0.0 6 2 115 9 0.0 6 8 1 116 2 0.0 6 2 117 6 0.0 6 6 118 1 0.0 6 1 119 3 0.0 6 3 120 3 0.0 6 2 1 121 6 0.0 6 6 122 18 0.0 6 18 123 6 0.0 6 5 1 124 8 0.0 6 8 125 7 0.0 6 7 126 7 0.0 6 7 127 10 0.0 6 10 128 15 0.0 6 15 129 9 0.0 6 9 130 11 0.0 6 10 1 131 2 0.0 6 2 132 10 0.0 6 10 133 29 0.0 6 29 134 26 0.0 6 26 135 11 0.0 6 11 136 11 0.0 6 11 137 7 0.0 6 7 138 10 0.0 6 10 139 10 0.0 6 10 140 9 0.0 6 9 141 6 0.0 6 6 142 17 0.0 6 17 143 14 0.0 6 14 144 21 0.0 6 20 1 145 22 0.0 6 22 146 16 0.0 6 15 1 147 5 0.0 6 4 1 148 19 0.0 6 18 0 0 1 149 9 0.0 6 8 1 150 24 0.0 6 23 1 151 8 0.0 6 8 152 18 0.0 6 16 2 153 25 0.0 6 24 1 154 7 0.0 6 6 0 1 155 6 0.0 6 6 156 1 0.0 6 1 157 11 0.0 6 11 158 21 0.0 6 21 159 7 0.0 6 6 1 160 8 0.0 6 8 161 37 0.0 6 35 2 162 23 0.0 6 22 1 163 20 0.0 6 19 1 164 9 0.0 6 9 165 15 0.0 6 14 1 166 11 0.0 6 11 167 9 0.0 6 9 168 12 0.0 6 11 1 169 14 0.0 6 14 170 9 0.0 6 9 171 23 0.0 6 21 2 172 24 0.0 6 23 1 173 17 0.0 6 17 174 33 0.0 6 33 175 22 0.0 6 20 2 176 15 0.0 6 14 1 177 27 0.0 6 26 0 1 178 26 0.0 6 26 179 27 0.0 6 26 1 180 10 0.0 6 10 181 25 0.0 6 24 1 182 34 0.0 6 33 1 183 19 0.0 6 18 1 184 32 0.0 6 32 185 50 0.0 6 47 3 186 47 0.0 6 47 187 49 0.0 6 48 1 188 75 0.0 6 74 1 189 68 0.0 6 66 1 1 190 32 0.0 6 32 191 39 0.0 6 37 2 192 23 0.0 6 23 193 46 0.0 6 45 1 194 35 0.0 6 34 1 195 43 0.0 6 41 1 1 196 71 0.0 6 67 4 197 56 0.0 6 52 4 198 82 0.0 6 81 1 199 88 0.0 6 86 2 200 66 0.0 6 63 3 201 88 0.0 6 85 2 1 202 104 0.0 6 101 3 203 93 0.0 6 92 1 204 64 0.0 6 59 5 205 134 0.0 6 132 2 206 143 0.0 6 136 7 207 436 0.0 6 422 14 208 311 0.0 6 301 10 209 227 0.0 6 224 3 210 432 0.0 6 419 13 211 406 0.0 6 398 8 212 272 0.0 6 262 9 1 213 188 0.0 6 183 5 214 203 0.0 6 200 3 215 83 0.0 6 82 1 216 133 0.0 6 129 4 217 134 0.0 6 133 1 218 171 0.0 6 168 3 219 122 0.0 6 118 4 220 197 0.0 6 191 6 221 163 0.0 6 157 6 222 208 0.0 6 205 2 1 223 122 0.0 6 120 2 224 88 0.0 6 86 2 225 90 0.0 6 86 4 226 157 0.0 6 151 6 227 289 0.0 6 285 3 1 228 224 0.0 6 219 5 229 108 0.0 6 105 3 230 224 0.0 6 223 1 231 368 0.0 6 357 11 232 134 0.0 6 132 2 233 212 0.0 6 207 5 234 245 0.0 6 241 3 1 235 138 0.0 6 135 2 0 1 236 382 0.0 6 377 4 1 237 79 0.0 6 78 1 238 340 0.0 6 334 6 239 157 0.0 6 152 5 240 134 0.0 6 128 6 241 218 0.0 6 214 4 242 178 0.0 6 174 3 1 243 218 0.0 6 210 7 1 244 435 0.0 6 428 7 245 450 0.0 6 443 7 246 311 0.0 6 301 10 247 141 0.0 6 138 2 1 248 523 0.0 6 514 9 249 401 0.0 6 385 16 250 1159 0.0 6 1148 11 251 564 0.0 6 550 14 252 405 0.0 6 388 16 0 1 253 756 0.0 6 731 22 3 254 1278 0.0 6 1254 23 1 255 1604 0.0 6 1583 20 1 256 741 0.0 6 712 28 0 0 1 257 1758 0.0 6 1739 19 258 580 0.0 6 565 15 259 1729 0.0 6 1697 31 0 0 1 260 1105 0.0 6 1083 20 2 261 2384 0.0 6 2344 39 1 262 497 0.0 6 486 10 0 1 263 431 0.0 6 416 14 0 0 0 1 264 741 0.0 6 718 23 265 1268 0.0 6 1248 20 266 476 0.0 6 456 20 267 1149 0.0 6 1126 21 2 268 484 0.0 6 466 17 1 269 814 0.0 6 801 13 270 317 0.0 6 305 12 271 601 0.0 6 589 12 272 465 0.0 6 448 15 1 0 1 273 472 0.0 6 462 9 0 1 274 1557 0.0 6 1523 32 2 275 1640 0.0 6 1604 36 276 2591 0.0 6 2533 56 2 277 3604 0.0 6 3551 52 1 278 3799 0.0 6 3732 64 3 279 3983 0.0 6 3910 72 1 280 10269 0.0 6 10016 248 2 2 1 281 5097 0.0 6 4985 104 8 282 32 0.0 6 28 3 1 283 15 0.0 6 13 2 284 2 0.0 6 0 0 0 0 1 1 285 2 0.0 6 1 0 0 0 0 1 286 3 0.0 6 3 287 1 0.0 6 1 288 3 0.0 6 3 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 1 0.0 6 1 125 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 67136 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 65.6 1 1 7 4 4.1 2 3 1 10 1 0.1 3 0 1 11 2 0.0 3 2 12 2 0.0 3 2 13 3 0.0 3 2 1 14 6 0.0 4 6 16 5 0.0 4 5 17 5 0.0 5 5 19 19 0.0 5 17 2 20 47 0.0 6 45 1 1 21 40 0.0 6 37 3 22 28 0.0 6 26 2 23 23 0.0 6 22 1 24 16 0.0 6 16 25 10 0.0 6 10 26 14 0.0 6 11 3 27 4 0.0 6 4 28 14 0.0 6 13 1 29 9 0.0 6 8 1 30 7 0.0 6 6 1 31 1 0.0 6 1 32 4 0.0 6 4 33 10 0.0 6 10 34 8 0.0 6 8 35 9 0.0 6 7 2 36 3 0.0 6 3 37 5 0.0 6 4 1 38 12 0.0 6 11 1 39 10 0.0 6 8 1 0 0 0 1 40 4 0.0 6 4 41 5 0.0 6 5 42 5 0.0 6 5 43 5 0.0 6 5 44 9 0.0 6 9 45 2 0.0 6 2 46 9 0.0 6 9 47 8 0.0 6 8 48 5 0.0 6 4 1 49 18 0.0 6 17 1 50 14 0.0 6 13 1 51 19 0.0 6 16 2 0 0 1 52 27 0.0 6 25 1 1 53 12 0.0 6 11 1 54 8 0.0 6 6 2 55 18 0.0 6 17 1 56 15 0.0 6 14 1 57 18 0.0 6 15 3 58 15 0.0 6 14 1 59 13 0.0 6 11 2 60 16 0.0 6 15 1 61 4 0.0 6 4 62 8 0.0 6 5 3 63 9 0.0 6 9 64 4 0.0 6 4 65 13 0.0 6 12 1 66 8 0.0 6 8 67 9 0.0 6 8 1 68 19 0.0 6 16 3 69 27 0.0 6 24 1 1 1 70 103 0.0 6 100 3 71 18 0.0 6 16 2 72 81 0.0 6 77 4 73 15 0.0 6 15 74 31 0.0 6 31 75 13 0.0 6 12 1 76 19 0.0 6 16 2 1 77 18 0.0 6 17 1 78 22 0.0 6 20 2 79 22 0.0 6 21 1 80 26 0.0 6 25 1 81 20 0.0 6 15 5 82 27 0.0 6 24 3 83 15 0.0 6 14 1 84 46 0.0 6 41 4 0 0 1 85 17 0.0 6 14 3 86 36 0.0 6 33 3 87 12 0.0 6 11 1 88 6 0.0 6 5 1 89 13 0.0 6 9 3 0 0 1 90 10 0.0 6 10 91 21 0.0 6 20 1 92 26 0.0 6 25 1 93 27 0.0 6 26 1 94 45 0.0 6 43 1 0 0 1 95 23 0.0 6 21 1 0 0 1 96 31 0.0 6 30 1 97 48 0.0 6 47 1 98 16 0.0 6 16 99 22 0.0 6 19 3 100 20 0.0 6 20 101 19 0.0 6 18 1 102 34 0.0 6 31 3 103 41 0.0 6 40 1 104 38 0.0 6 37 1 105 47 0.0 6 39 8 106 29 0.0 6 24 4 0 0 1 107 51 0.0 6 48 2 0 0 1 108 27 0.0 6 27 109 14 0.0 6 13 1 110 26 0.0 6 23 3 111 13 0.0 6 11 2 112 4 0.0 6 4 113 10 0.0 6 9 1 114 15 0.0 6 12 3 115 41 0.0 6 39 2 116 48 0.0 6 46 2 117 34 0.0 6 33 1 118 18 0.0 6 17 1 119 62 0.0 6 57 5 120 33 0.0 6 31 0 1 0 0 1 121 40 0.0 6 38 2 122 39 0.0 6 34 5 123 85 0.0 6 79 5 0 0 1 124 50 0.0 6 47 3 125 54 0.0 6 50 4 126 81 0.0 6 76 5 127 91 0.0 6 84 5 1 0 1 128 45 0.0 6 43 2 129 92 0.0 6 87 5 130 64 0.0 6 59 5 131 107 0.0 6 102 5 132 49 0.0 6 47 2 133 46 0.0 6 41 4 0 0 1 134 77 0.0 6 73 3 1 135 114 0.0 6 109 3 2 136 134 0.0 6 132 1 1 137 82 0.0 6 78 3 0 0 1 138 201 0.0 6 191 10 139 103 0.0 6 101 2 140 114 0.0 6 104 9 0 0 1 141 188 0.0 6 179 9 142 136 0.0 6 131 5 143 226 0.0 6 213 13 144 154 0.0 6 146 7 0 1 145 75 0.0 6 68 6 1 146 161 0.0 6 157 4 147 101 0.0 6 98 3 148 194 0.0 6 189 4 0 0 1 149 191 0.0 6 187 3 1 150 245 0.0 6 239 6 151 194 0.0 6 187 6 1 152 253 0.0 6 246 6 0 0 0 1 153 251 0.0 6 241 10 154 249 0.0 6 239 9 0 1 155 595 0.0 6 569 22 1 1 1 1 156 709 0.0 6 689 20 157 441 0.0 6 423 17 1 158 269 0.0 6 256 13 159 260 0.0 6 254 6 160 220 0.0 6 207 12 0 0 1 161 274 0.0 6 261 11 2 162 281 0.0 6 271 8 2 163 422 0.0 6 410 12 164 895 0.0 6 881 13 1 165 301 0.0 6 296 4 0 0 1 166 596 0.0 6 575 20 1 167 273 0.0 6 260 12 0 1 168 239 0.0 6 232 7 169 422 0.0 6 408 11 0 0 1 1 1 170 436 0.0 6 426 10 171 597 0.0 6 581 15 1 172 850 0.0 6 825 23 1 0 1 173 250 0.0 6 242 7 1 174 881 0.0 6 856 24 0 1 175 726 0.0 6 704 19 1 0 2 176 245 0.0 6 236 8 1 177 323 0.0 6 311 11 1 178 274 0.0 6 264 10 179 530 0.0 6 512 18 180 249 0.0 6 242 5 2 181 345 0.0 6 332 11 2 182 246 0.0 6 238 7 0 1 183 606 0.0 6 592 13 0 0 1 184 222 0.0 6 211 9 0 2 185 551 0.0 6 534 15 0 1 1 186 321 0.0 6 308 12 1 187 818 0.0 6 795 23 188 820 0.0 6 799 19 1 0 0 1 189 986 0.0 6 964 21 0 0 1 190 505 0.0 6 492 13 191 663 0.0 6 646 17 192 846 0.0 6 813 31 0 0 1 1 193 665 0.0 6 643 20 2 194 1035 0.0 6 1005 26 3 0 1 195 586 0.0 6 573 12 1 196 803 0.0 6 786 16 0 0 1 197 375 0.0 6 352 22 1 198 962 0.0 6 937 24 0 0 1 199 1528 0.0 6 1495 30 2 0 1 200 1315 0.0 6 1297 17 0 0 1 201 804 0.0 6 777 26 1 202 806 0.0 6 784 22 203 800 0.0 6 780 20 204 574 0.0 6 557 16 1 205 559 0.0 6 539 19 1 206 1207 0.0 6 1181 26 207 227 0.0 6 223 3 0 0 1 208 516 0.0 6 509 7 209 668 0.0 6 659 8 1 210 421 0.0 6 407 14 211 194 0.0 6 183 11 212 509 0.0 6 493 15 0 1 213 128 0.0 6 121 7 214 264 0.0 6 261 3 215 227 0.0 6 218 9 216 565 0.0 6 555 10 217 368 0.0 6 354 13 1 218 491 0.0 6 478 13 219 728 0.0 6 711 17 220 290 0.0 6 278 10 0 1 1 221 259 0.0 6 250 9 222 363 0.0 6 346 13 3 0 0 1 223 1200 0.0 6 1157 42 0 0 1 224 3500 0.0 6 3440 56 1 1 1 1 225 1357 0.0 6 1328 27 2 226 1208 0.0 6 1162 41 2 2 1 227 5024 0.0 6 4920 102 0 0 2 228 588 0.0 6 567 21 229 1898 0.0 6 1873 23 1 0 1 230 319 0.0 6 303 16 231 884 0.0 6 864 16 2 1 1 232 815 0.0 6 788 25 0 2 233 987 0.0 6 956 29 0 1 1 234 1265 0.0 6 1236 27 0 1 0 1 235 597 0.0 6 585 12 236 74 0.0 6 69 4 1 237 130 0.0 6 128 2 238 209 0.0 6 209 239 64 0.0 6 64 240 28 0.0 6 27 0 0 0 1 241 228 0.0 6 224 3 0 0 1 242 91 0.0 6 89 2 243 155 0.0 6 150 5 244 776 0.0 6 762 14 245 64 0.0 6 60 3 0 0 1 246 104 0.0 6 102 2 247 77 0.0 6 77 248 145 0.0 6 140 5 249 187 0.0 6 184 3 250 67 0.0 6 63 3 1 251 377 0.0 6 362 15 252 258 0.0 6 254 4 253 136 0.0 6 134 1 0 0 1 254 342 0.0 6 333 9 255 20 0.0 6 20 256 21 0.0 6 17 3 0 1 257 10 0.0 6 9 1 258 3 0.0 6 3 267 1 0.0 6 1 275 1 0.0 6 1 276 1 0.0 6 1 278 3 0.0 6 3 279 9 0.0 6 9 280 9 0.0 6 9 281 124 0.0 6 118 6 282 649 0.0 6 631 16 1 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 6 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 75 1 0.0 6 1 90 1 0.0 6 1 113 1 0.0 6 0 0 0 0 0 1 124 2 0.0 6 2 221 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_18_141_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_18_368_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_18_141_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_18_368_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.33 s (35 µs/read; 1.71 M reads/minute). === Summary === Total read pairs processed: 66,525 Read 1 with adapter: 66,517 (100.0%) Read 2 with adapter: 66,458 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 74 (0.1%) Pairs written (passing filters): 66,451 (99.9%) Total basepairs processed: 34,225,011 bp Read 1: 19,681,656 bp Read 2: 14,543,355 bp Total written (filtered): 4,125,277 bp (12.1%) Read 1: 1,996,729 bp Read 2: 2,128,548 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 66464 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1039.5 0 1 1 4 1 259.9 1 0 1 7 1 4.1 2 0 1 9 1 0.3 2 1 15 1 0.0 4 1 16 3 0.0 4 3 17 1 0.0 5 0 1 19 1 0.0 5 1 20 9 0.0 6 8 1 21 8 0.0 6 8 22 5 0.0 6 5 23 1 0.0 6 1 24 3 0.0 6 3 25 5 0.0 6 4 1 26 4 0.0 6 4 27 1 0.0 6 0 1 28 2 0.0 6 2 29 1 0.0 6 0 1 30 2 0.0 6 2 31 1 0.0 6 1 32 1 0.0 6 0 1 33 3 0.0 6 3 34 2 0.0 6 2 36 1 0.0 6 0 1 37 1 0.0 6 1 39 1 0.0 6 1 40 6 0.0 6 4 1 1 41 2 0.0 6 1 0 0 0 0 1 42 1 0.0 6 1 44 3 0.0 6 3 45 4 0.0 6 4 46 5 0.0 6 5 47 1 0.0 6 1 48 1 0.0 6 1 49 1 0.0 6 1 50 1 0.0 6 1 52 1 0.0 6 0 1 53 1 0.0 6 1 54 1 0.0 6 1 55 1 0.0 6 1 56 5 0.0 6 5 57 4 0.0 6 2 2 58 2 0.0 6 2 59 4 0.0 6 3 1 60 1 0.0 6 1 62 4 0.0 6 4 63 3 0.0 6 3 64 2 0.0 6 2 65 2 0.0 6 2 66 3 0.0 6 3 68 1 0.0 6 1 69 1 0.0 6 1 70 1 0.0 6 1 71 1 0.0 6 1 72 3 0.0 6 3 73 2 0.0 6 2 75 1 0.0 6 1 76 2 0.0 6 1 0 1 77 2 0.0 6 2 78 5 0.0 6 5 79 3 0.0 6 3 80 1 0.0 6 1 81 3 0.0 6 3 82 1 0.0 6 1 83 2 0.0 6 1 1 84 1 0.0 6 1 85 1 0.0 6 1 86 8 0.0 6 7 1 87 4 0.0 6 3 1 88 1 0.0 6 1 89 6 0.0 6 6 91 3 0.0 6 3 92 5 0.0 6 4 1 93 2 0.0 6 2 96 2 0.0 6 2 97 4 0.0 6 4 98 4 0.0 6 4 99 1 0.0 6 1 100 3 0.0 6 3 101 12 0.0 6 12 102 5 0.0 6 4 0 0 1 103 5 0.0 6 5 104 5 0.0 6 5 105 7 0.0 6 6 1 106 4 0.0 6 4 107 6 0.0 6 4 1 1 108 5 0.0 6 5 109 4 0.0 6 4 110 2 0.0 6 2 111 5 0.0 6 5 112 3 0.0 6 2 1 113 6 0.0 6 6 114 6 0.0 6 6 115 5 0.0 6 5 116 3 0.0 6 3 117 5 0.0 6 5 118 4 0.0 6 4 119 4 0.0 6 4 120 5 0.0 6 5 121 4 0.0 6 3 1 122 8 0.0 6 5 3 123 13 0.0 6 13 124 8 0.0 6 8 125 3 0.0 6 2 1 126 7 0.0 6 7 127 4 0.0 6 4 128 6 0.0 6 6 129 8 0.0 6 6 2 130 10 0.0 6 10 131 10 0.0 6 8 2 132 20 0.0 6 20 133 15 0.0 6 14 1 134 19 0.0 6 17 1 1 135 19 0.0 6 19 136 7 0.0 6 7 137 9 0.0 6 9 138 12 0.0 6 12 139 3 0.0 6 3 140 10 0.0 6 10 141 15 0.0 6 15 142 13 0.0 6 12 0 1 143 11 0.0 6 9 2 144 28 0.0 6 28 145 61 0.0 6 61 146 14 0.0 6 14 147 14 0.0 6 13 1 148 27 0.0 6 27 149 159 0.0 6 159 150 31 0.0 6 31 151 10 0.0 6 9 1 152 22 0.0 6 21 1 153 161 0.0 6 160 1 154 32 0.0 6 32 155 11 0.0 6 11 156 79 0.0 6 78 1 157 32 0.0 6 32 158 12 0.0 6 12 159 10 0.0 6 9 1 160 24 0.0 6 23 1 161 15 0.0 6 15 162 15 0.0 6 15 163 13 0.0 6 13 164 15 0.0 6 15 165 29 0.0 6 29 166 14 0.0 6 14 167 24 0.0 6 23 1 168 50 0.0 6 47 3 169 309 0.0 6 306 3 170 45 0.0 6 45 171 17 0.0 6 17 172 30 0.0 6 30 173 32 0.0 6 32 174 32 0.0 6 32 175 33 0.0 6 28 4 1 176 28 0.0 6 27 1 177 41 0.0 6 40 1 178 25 0.0 6 25 179 18 0.0 6 17 1 180 30 0.0 6 29 1 181 44 0.0 6 41 3 182 28 0.0 6 27 1 183 43 0.0 6 38 5 184 55 0.0 6 54 1 185 70 0.0 6 70 186 67 0.0 6 66 1 187 51 0.0 6 50 1 188 102 0.0 6 97 5 189 107 0.0 6 103 4 190 53 0.0 6 49 4 191 89 0.0 6 87 2 192 91 0.0 6 86 4 1 193 90 0.0 6 89 1 194 72 0.0 6 69 3 195 93 0.0 6 92 1 196 135 0.0 6 131 4 197 106 0.0 6 102 4 198 143 0.0 6 137 5 1 199 156 0.0 6 154 2 200 132 0.0 6 127 4 1 201 114 0.0 6 107 6 1 202 151 0.0 6 147 4 203 171 0.0 6 165 6 204 135 0.0 6 132 3 205 180 0.0 6 176 4 206 132 0.0 6 126 6 207 168 0.0 6 163 5 208 202 0.0 6 196 6 209 173 0.0 6 166 7 210 141 0.0 6 137 4 211 104 0.0 6 101 2 1 212 76 0.0 6 75 1 213 130 0.0 6 124 5 0 1 214 157 0.0 6 149 8 215 113 0.0 6 106 7 216 139 0.0 6 136 3 217 244 0.0 6 240 4 218 164 0.0 6 158 6 219 102 0.0 6 97 5 220 134 0.0 6 128 5 1 221 174 0.0 6 164 10 222 165 0.0 6 162 3 223 146 0.0 6 141 5 224 115 0.0 6 113 2 225 147 0.0 6 141 6 226 227 0.0 6 222 5 227 205 0.0 6 199 5 1 228 194 0.0 6 192 2 229 152 0.0 6 146 6 230 125 0.0 6 121 4 231 129 0.0 6 123 6 232 121 0.0 6 113 8 233 120 0.0 6 116 4 234 125 0.0 6 120 5 235 120 0.0 6 119 1 236 161 0.0 6 159 2 237 177 0.0 6 172 5 238 151 0.0 6 148 3 239 191 0.0 6 183 8 240 225 0.0 6 209 14 2 241 366 0.0 6 350 14 1 0 1 242 280 0.0 6 260 19 1 243 281 0.0 6 273 8 244 206 0.0 6 192 14 245 313 0.0 6 306 7 246 129 0.0 6 119 10 247 152 0.0 6 145 7 248 139 0.0 6 133 6 249 273 0.0 6 263 10 250 204 0.0 6 199 5 251 221 0.0 6 212 8 1 252 183 0.0 6 177 6 253 129 0.0 6 122 6 1 254 234 0.0 6 228 6 255 159 0.0 6 151 8 256 191 0.0 6 183 7 1 257 168 0.0 6 159 9 258 133 0.0 6 128 5 259 278 0.0 6 254 23 0 1 260 290 0.0 6 284 5 1 261 148 0.0 6 134 13 1 262 220 0.0 6 204 16 263 293 0.0 6 282 7 2 0 2 264 217 0.0 6 204 13 265 214 0.0 6 211 3 266 118 0.0 6 110 8 267 163 0.0 6 156 6 1 268 203 0.0 6 183 19 1 269 274 0.0 6 264 10 270 124 0.0 6 116 8 271 332 0.0 6 312 20 272 548 0.0 6 524 23 1 273 314 0.0 6 297 17 274 490 0.0 6 478 10 2 275 249 0.0 6 223 25 1 276 645 0.0 6 611 34 277 1720 0.0 6 1677 40 3 278 807 0.0 6 785 22 279 2041 0.0 6 1976 64 1 280 2933 0.0 6 2697 218 14 4 281 40135 0.0 6 39302 817 11 3 0 1 1 282 35 0.0 6 28 3 2 2 283 6 0.0 6 6 284 5 0.0 6 3 1 0 0 1 286 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 53 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 64 1 0.0 6 0 0 0 0 0 0 1 72 1 0.0 6 1 73 1 0.0 6 1 75 1 0.0 6 1 76 9 0.0 6 9 79 1 0.0 6 1 80 5 0.0 6 5 83 2 0.0 6 2 95 4 0.0 6 4 96 6 0.0 6 6 134 2 0.0 6 0 0 0 0 0 0 2 137 1 0.0 6 0 0 0 0 0 0 1 152 1 0.0 6 1 162 1 0.0 6 1 163 8 0.0 6 8 178 3 0.0 6 3 181 1 0.0 6 1 191 2 0.0 6 2 192 1 0.0 6 1 204 1 0.0 6 1 205 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 66391 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 16.2 1 1 7 1 4.1 2 0 1 9 1 0.3 2 0 1 10 3 0.1 3 2 1 11 1 0.0 3 1 12 1 0.0 3 0 1 13 4 0.0 3 4 14 9 0.0 4 9 15 1 0.0 4 1 16 11 0.0 4 9 2 17 17 0.0 5 15 2 18 13 0.0 5 13 19 13 0.0 5 10 2 0 1 20 65 0.0 6 64 0 1 21 68 0.0 6 63 5 22 27 0.0 6 26 1 23 23 0.0 6 20 2 1 24 13 0.0 6 13 25 19 0.0 6 18 1 26 14 0.0 6 12 2 27 18 0.0 6 17 1 28 9 0.0 6 7 2 29 10 0.0 6 10 30 20 0.0 6 17 3 31 8 0.0 6 7 1 32 6 0.0 6 6 33 11 0.0 6 11 34 16 0.0 6 16 35 9 0.0 6 7 2 36 7 0.0 6 6 0 0 0 1 37 18 0.0 6 17 1 38 6 0.0 6 6 39 9 0.0 6 9 40 8 0.0 6 6 2 41 8 0.0 6 7 1 42 8 0.0 6 8 43 15 0.0 6 13 2 44 25 0.0 6 23 2 45 9 0.0 6 8 1 46 31 0.0 6 24 5 2 47 22 0.0 6 20 2 48 12 0.0 6 10 2 49 16 0.0 6 15 1 50 18 0.0 6 16 2 51 9 0.0 6 8 1 52 21 0.0 6 17 2 2 53 35 0.0 6 27 7 0 0 1 54 38 0.0 6 28 8 2 55 22 0.0 6 18 4 56 15 0.0 6 12 3 57 21 0.0 6 17 3 1 58 13 0.0 6 11 1 1 59 14 0.0 6 11 3 60 17 0.0 6 16 1 61 7 0.0 6 6 1 62 12 0.0 6 10 2 63 20 0.0 6 18 2 64 10 0.0 6 8 2 65 9 0.0 6 7 2 66 19 0.0 6 17 1 1 67 143 0.0 6 139 2 1 1 68 34 0.0 6 28 4 2 69 40 0.0 6 38 2 70 10 0.0 6 10 71 33 0.0 6 30 3 72 17 0.0 6 16 1 73 16 0.0 6 13 3 74 17 0.0 6 16 1 75 8 0.0 6 6 2 76 19 0.0 6 17 2 77 8 0.0 6 5 3 78 12 0.0 6 8 4 79 13 0.0 6 11 2 80 22 0.0 6 18 4 81 19 0.0 6 14 2 2 0 1 82 21 0.0 6 19 2 83 51 0.0 6 46 4 0 0 1 84 28 0.0 6 27 1 85 30 0.0 6 26 3 1 86 22 0.0 6 20 2 87 36 0.0 6 29 7 88 20 0.0 6 17 3 89 47 0.0 6 43 4 90 28 0.0 6 26 1 1 91 35 0.0 6 29 6 92 25 0.0 6 23 2 93 54 0.0 6 46 6 2 94 82 0.0 6 74 7 1 95 32 0.0 6 26 6 96 39 0.0 6 36 2 1 97 30 0.0 6 29 1 98 30 0.0 6 27 2 0 0 1 99 50 0.0 6 44 5 0 0 1 100 38 0.0 6 34 3 1 101 40 0.0 6 35 5 102 82 0.0 6 74 7 1 103 79 0.0 6 74 5 104 78 0.0 6 71 7 105 53 0.0 6 49 4 106 39 0.0 6 34 5 107 41 0.0 6 38 3 108 43 0.0 6 38 5 109 52 0.0 6 50 2 110 67 0.0 6 64 2 1 111 59 0.0 6 54 5 112 58 0.0 6 50 7 0 0 1 113 68 0.0 6 62 6 114 59 0.0 6 53 5 0 0 1 115 33 0.0 6 29 4 116 41 0.0 6 39 2 117 46 0.0 6 43 3 118 58 0.0 6 53 5 119 62 0.0 6 60 2 120 61 0.0 6 56 5 121 46 0.0 6 42 4 122 56 0.0 6 52 4 123 42 0.0 6 40 1 1 124 75 0.0 6 74 1 125 66 0.0 6 57 7 1 0 0 0 1 126 69 0.0 6 59 10 127 77 0.0 6 75 2 128 37 0.0 6 34 3 129 36 0.0 6 35 1 130 53 0.0 6 51 1 0 0 1 131 107 0.0 6 103 3 1 132 99 0.0 6 95 4 133 97 0.0 6 86 11 134 190 0.0 6 180 9 0 1 135 255 0.0 6 246 9 136 92 0.0 6 85 7 137 130 0.0 6 125 5 138 59 0.0 6 56 2 0 1 139 132 0.0 6 124 8 140 268 0.0 6 254 11 3 141 196 0.0 6 183 12 0 0 1 142 287 0.0 6 276 10 0 0 1 143 239 0.0 6 227 11 1 144 424 0.0 6 407 12 3 0 2 145 182 0.0 6 173 5 2 0 2 146 190 0.0 6 182 7 1 147 118 0.0 6 113 5 148 290 0.0 6 283 7 149 387 0.0 6 359 24 1 1 2 150 584 0.0 6 554 26 1 0 3 151 216 0.0 6 207 6 2 1 152 663 0.0 6 641 20 2 153 311 0.0 6 298 13 154 340 0.0 6 331 8 1 155 293 0.0 6 277 14 1 0 1 156 238 0.0 6 227 11 157 361 0.0 6 350 11 158 341 0.0 6 331 10 159 209 0.0 6 202 7 160 328 0.0 6 313 13 1 1 161 193 0.0 6 181 11 1 162 190 0.0 6 186 4 163 400 0.0 6 383 14 2 0 0 1 164 434 0.0 6 425 9 165 371 0.0 6 355 15 1 166 365 0.0 6 354 10 0 0 1 167 273 0.0 6 257 15 1 168 505 0.0 6 483 20 0 1 1 169 195 0.0 6 188 6 0 1 170 238 0.0 6 226 12 171 497 0.0 6 481 16 172 262 0.0 6 250 10 2 173 459 0.0 6 439 18 2 174 699 0.0 6 662 33 1 2 1 175 888 0.0 6 857 30 1 176 701 0.0 6 671 26 3 0 0 1 177 584 0.0 6 564 20 178 439 0.0 6 419 16 2 2 179 528 0.0 6 497 31 180 479 0.0 6 451 27 0 1 181 586 0.0 6 569 17 182 214 0.0 6 198 16 183 795 0.0 6 761 33 1 184 543 0.0 6 515 25 2 0 1 185 810 0.0 6 775 33 1 1 186 1512 0.0 6 1465 43 3 0 1 187 1222 0.0 6 1179 42 1 188 1136 0.0 6 1095 39 1 1 189 984 0.0 6 933 47 1 1 2 190 1697 0.0 6 1636 58 2 1 191 1891 0.0 6 1838 51 1 0 1 192 900 0.0 6 861 35 1 0 3 193 560 0.0 6 525 30 2 0 2 0 1 194 1030 0.0 6 992 35 2 0 1 195 1450 0.0 6 1394 54 2 196 2572 0.0 6 2484 85 0 0 3 197 994 0.0 6 955 37 2 198 1818 0.0 6 1736 74 4 0 3 1 199 2870 0.0 6 2776 89 0 0 5 200 1310 0.0 6 1264 46 201 1709 0.0 6 1650 55 1 0 3 202 762 0.0 6 733 27 1 1 203 744 0.0 6 723 20 1 204 1274 0.0 6 1236 38 205 348 0.0 6 340 7 0 0 1 206 423 0.0 6 410 11 0 0 2 207 226 0.0 6 217 6 2 0 1 208 467 0.0 6 448 19 209 503 0.0 6 478 22 2 0 0 1 210 511 0.0 6 499 12 211 399 0.0 6 383 13 2 0 1 212 815 0.0 6 785 29 0 0 1 213 469 0.0 6 452 17 214 1719 0.0 6 1662 57 215 740 0.0 6 715 24 1 216 660 0.0 6 635 24 1 217 592 0.0 6 567 21 2 1 1 218 411 0.0 6 391 20 219 648 0.0 6 629 19 220 137 0.0 6 130 6 1 221 515 0.0 6 498 15 1 0 0 1 222 569 0.0 6 547 21 0 1 223 804 0.0 6 784 19 0 0 1 224 426 0.0 6 406 20 225 608 0.0 6 593 14 0 0 1 226 444 0.0 6 437 7 227 440 0.0 6 429 11 228 240 0.0 6 230 8 1 1 229 423 0.0 6 415 7 1 230 245 0.0 6 236 9 231 420 0.0 6 409 11 232 451 0.0 6 432 18 1 233 280 0.0 6 278 1 0 0 1 234 181 0.0 6 174 7 235 101 0.0 6 99 1 0 0 0 1 236 21 0.0 6 21 237 36 0.0 6 33 3 238 52 0.0 6 50 2 239 60 0.0 6 58 2 240 157 0.0 6 153 4 241 165 0.0 6 158 6 1 242 241 0.0 6 239 2 243 212 0.0 6 206 6 244 147 0.0 6 141 6 245 118 0.0 6 114 4 246 153 0.0 6 148 5 247 45 0.0 6 42 3 248 142 0.0 6 140 2 249 30 0.0 6 28 2 250 22 0.0 6 22 251 61 0.0 6 59 2 252 6 0.0 6 5 1 253 6 0.0 6 6 254 3 0.0 6 3 255 3 0.0 6 3 257 1 0.0 6 1 258 1 0.0 6 1 259 1 0.0 6 1 275 1 0.0 6 1 276 1 0.0 6 1 277 5 0.0 6 4 0 0 0 0 1 278 37 0.0 6 37 279 49 0.0 6 45 3 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 67 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 74 2 0.0 6 2 77 2 0.0 6 2 78 7 0.0 6 7 79 1 0.0 6 1 81 4 0.0 6 4 82 5 0.0 6 5 85 4 0.0 6 4 97 3 0.0 6 3 98 17 0.0 6 17 118 1 0.0 6 1 142 1 0.0 6 1 147 1 0.0 6 1 164 4 0.0 6 4 165 10 0.0 6 10 166 1 0.0 6 1 179 2 0.0 6 2 180 1 0.0 6 1 190 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_19_142_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_19_369_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_19_142_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_19_369_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.36 s (33 µs/read; 1.82 M reads/minute). === Summary === Total read pairs processed: 71,854 Read 1 with adapter: 71,848 (100.0%) Read 2 with adapter: 71,815 (99.9%) == Read fate breakdown == Pairs that were too short: 3 (0.0%) Pairs discarded as untrimmed: 45 (0.1%) Pairs written (passing filters): 71,806 (99.9%) Total basepairs processed: 35,210,911 bp Read 1: 19,878,216 bp Read 2: 15,332,695 bp Total written (filtered): 4,338,212 bp (12.3%) Read 1: 2,173,901 bp Read 2: 2,164,311 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 71503 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1122.7 0 2 4 1 280.7 1 0 1 6 2 17.5 1 1 0 1 7 1 4.4 2 1 8 1 1.1 2 0 0 1 9 4 0.3 2 2 2 10 2 0.1 3 2 15 3 0.0 4 3 16 1 0.0 4 0 1 17 1 0.0 5 1 19 3 0.0 5 1 2 20 40 0.0 6 40 21 22 0.0 6 22 22 26 0.0 6 26 23 5 0.0 6 5 24 9 0.0 6 8 1 25 15 0.0 6 15 26 7 0.0 6 7 27 5 0.0 6 5 28 6 0.0 6 5 1 29 5 0.0 6 5 30 1 0.0 6 1 31 6 0.0 6 5 1 32 5 0.0 6 5 34 5 0.0 6 5 36 2 0.0 6 2 37 1 0.0 6 1 38 2 0.0 6 2 39 4 0.0 6 4 40 8 0.0 6 8 41 3 0.0 6 3 42 1 0.0 6 1 43 2 0.0 6 1 0 1 44 1 0.0 6 1 45 7 0.0 6 7 46 6 0.0 6 5 1 47 5 0.0 6 5 50 1 0.0 6 0 0 0 0 0 1 51 4 0.0 6 3 1 52 5 0.0 6 5 53 2 0.0 6 2 54 2 0.0 6 2 55 2 0.0 6 2 56 2 0.0 6 2 57 2 0.0 6 1 1 58 4 0.0 6 4 59 3 0.0 6 3 60 2 0.0 6 2 61 2 0.0 6 2 63 1 0.0 6 1 64 3 0.0 6 3 65 2 0.0 6 2 66 2 0.0 6 1 1 67 3 0.0 6 3 68 2 0.0 6 2 69 2 0.0 6 1 0 0 0 0 0 1 70 2 0.0 6 2 72 4 0.0 6 4 73 6 0.0 6 6 74 1 0.0 6 1 75 1 0.0 6 1 77 8 0.0 6 8 78 7 0.0 6 6 1 79 7 0.0 6 7 80 2 0.0 6 1 1 82 2 0.0 6 2 83 3 0.0 6 3 84 5 0.0 6 5 85 2 0.0 6 2 86 7 0.0 6 7 87 7 0.0 6 7 88 2 0.0 6 2 89 4 0.0 6 4 90 6 0.0 6 6 91 3 0.0 6 3 92 3 0.0 6 3 93 2 0.0 6 2 94 2 0.0 6 2 96 6 0.0 6 6 97 7 0.0 6 7 98 3 0.0 6 1 2 99 5 0.0 6 5 100 5 0.0 6 5 101 11 0.0 6 11 102 14 0.0 6 13 0 0 1 103 7 0.0 6 6 1 104 7 0.0 6 7 105 8 0.0 6 7 1 106 7 0.0 6 7 107 5 0.0 6 4 1 108 10 0.0 6 9 1 109 8 0.0 6 6 2 110 6 0.0 6 5 1 111 5 0.0 6 5 112 9 0.0 6 9 113 4 0.0 6 4 114 17 0.0 6 17 115 7 0.0 6 7 116 3 0.0 6 3 117 7 0.0 6 7 118 9 0.0 6 8 1 119 9 0.0 6 9 120 5 0.0 6 5 121 10 0.0 6 10 122 16 0.0 6 16 123 15 0.0 6 13 2 124 9 0.0 6 8 0 1 125 9 0.0 6 8 1 126 12 0.0 6 12 127 8 0.0 6 8 128 32 0.0 6 32 129 16 0.0 6 14 1 0 0 0 0 1 130 13 0.0 6 12 1 131 11 0.0 6 11 132 20 0.0 6 20 133 8 0.0 6 8 134 18 0.0 6 18 135 19 0.0 6 19 136 25 0.0 6 25 137 35 0.0 6 35 138 17 0.0 6 16 1 139 25 0.0 6 25 140 30 0.0 6 30 141 108 0.0 6 108 142 131 0.0 6 131 143 186 0.0 6 185 1 144 109 0.0 6 109 145 88 0.0 6 86 2 146 130 0.0 6 130 147 132 0.0 6 130 0 1 1 148 203 0.0 6 203 149 142 0.0 6 141 1 150 106 0.0 6 106 151 165 0.0 6 165 152 105 0.0 6 105 153 128 0.0 6 127 1 154 101 0.0 6 101 155 104 0.0 6 104 156 93 0.0 6 93 157 120 0.0 6 118 2 158 122 0.0 6 121 1 159 88 0.0 6 87 1 160 153 0.0 6 150 3 161 148 0.0 6 147 1 162 177 0.0 6 176 1 163 107 0.0 6 106 1 164 111 0.0 6 111 165 107 0.0 6 102 4 0 0 1 166 106 0.0 6 105 1 167 107 0.0 6 107 168 119 0.0 6 118 1 169 112 0.0 6 110 2 170 166 0.0 6 163 2 1 171 98 0.0 6 97 1 172 106 0.0 6 103 3 173 142 0.0 6 141 1 174 175 0.0 6 172 3 175 90 0.0 6 88 2 176 104 0.0 6 104 177 98 0.0 6 96 2 178 150 0.0 6 148 1 0 0 0 1 179 317 0.0 6 316 1 180 135 0.0 6 134 1 181 134 0.0 6 132 2 182 155 0.0 6 154 1 183 125 0.0 6 123 2 184 116 0.0 6 114 2 185 164 0.0 6 164 186 195 0.0 6 191 4 187 182 0.0 6 178 3 0 0 0 0 1 188 229 0.0 6 224 5 189 182 0.0 6 180 2 190 164 0.0 6 160 4 191 219 0.0 6 213 6 192 188 0.0 6 184 4 193 221 0.0 6 219 2 194 266 0.0 6 260 6 195 208 0.0 6 206 2 196 184 0.0 6 181 3 197 251 0.0 6 248 3 198 282 0.0 6 272 10 199 278 0.0 6 272 5 1 200 297 0.0 6 296 1 201 305 0.0 6 301 4 202 264 0.0 6 253 11 203 364 0.0 6 359 4 1 204 280 0.0 6 273 6 1 205 314 0.0 6 308 5 0 0 0 1 206 283 0.0 6 276 7 207 290 0.0 6 283 7 208 340 0.0 6 336 4 209 372 0.0 6 368 4 210 302 0.0 6 300 2 211 376 0.0 6 371 4 0 1 212 283 0.0 6 277 6 213 275 0.0 6 267 8 214 521 0.0 6 513 6 2 215 362 0.0 6 352 9 1 216 554 0.0 6 546 8 217 344 0.0 6 337 7 218 311 0.0 6 309 2 219 348 0.0 6 343 5 220 452 0.0 6 452 221 241 0.0 6 236 4 0 0 1 222 344 0.0 6 338 5 1 223 350 0.0 6 340 10 224 518 0.0 6 510 8 225 363 0.0 6 358 5 226 725 0.0 6 717 6 2 227 444 0.0 6 438 6 228 430 0.0 6 422 8 229 380 0.0 6 378 2 230 275 0.0 6 271 4 231 309 0.0 6 305 4 232 283 0.0 6 279 4 233 208 0.0 6 204 4 234 231 0.0 6 230 1 235 261 0.0 6 256 5 236 228 0.0 6 222 6 237 230 0.0 6 223 7 238 338 0.0 6 329 9 239 519 0.0 6 507 12 240 735 0.0 6 720 14 1 241 346 0.0 6 338 8 242 368 0.0 6 362 6 243 401 0.0 6 396 5 244 246 0.0 6 239 7 245 298 0.0 6 290 8 246 369 0.0 6 362 6 0 0 0 0 1 247 540 0.0 6 533 7 248 273 0.0 6 266 7 249 275 0.0 6 269 6 250 399 0.0 6 390 9 251 370 0.0 6 349 21 252 788 0.0 6 776 12 253 292 0.0 6 285 6 0 0 0 1 254 248 0.0 6 238 10 255 430 0.0 6 421 9 256 438 0.0 6 426 12 257 774 0.0 6 756 16 2 258 571 0.0 6 549 21 1 259 1027 0.0 6 1004 21 1 0 1 260 594 0.0 6 568 25 1 261 1168 0.0 6 1136 30 1 0 1 262 513 0.0 6 486 27 263 799 0.0 6 783 16 264 314 0.0 6 307 7 265 347 0.0 6 342 5 266 426 0.0 6 412 13 1 267 764 0.0 6 745 19 268 247 0.0 6 227 20 269 726 0.0 6 713 11 1 0 1 270 821 0.0 6 804 17 271 724 0.0 6 707 16 1 272 565 0.0 6 549 16 273 337 0.0 6 323 13 1 274 601 0.0 6 588 13 275 998 0.0 6 973 25 276 1077 0.0 6 1037 39 1 277 1597 0.0 6 1557 38 1 0 0 0 1 278 2193 0.0 6 2138 51 3 1 279 4204 0.0 6 4104 98 2 280 14321 0.0 6 14000 313 6 2 281 5220 0.0 6 5146 72 2 282 19 0.0 6 16 2 0 1 283 5 0.0 6 3 0 0 2 284 1 0.0 6 1 285 3 0.0 6 3 286 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 345 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 2 0.0 6 1 1 61 35 0.0 6 35 69 1 0.0 6 1 70 2 0.0 6 2 71 5 0.0 6 5 72 2 0.0 6 2 74 3 0.0 6 3 75 3 0.0 6 3 76 3 0.0 6 3 77 3 0.0 6 3 78 1 0.0 6 1 79 3 0.0 6 3 80 4 0.0 6 4 81 3 0.0 6 3 83 2 0.0 6 2 85 2 0.0 6 2 86 2 0.0 6 2 87 5 0.0 6 5 88 1 0.0 6 1 89 3 0.0 6 3 90 4 0.0 6 4 91 4 0.0 6 4 92 3 0.0 6 2 0 1 93 3 0.0 6 3 94 1 0.0 6 1 95 1 0.0 6 1 96 7 0.0 6 7 97 1 0.0 6 1 98 1 0.0 6 1 99 3 0.0 6 3 102 1 0.0 6 1 103 2 0.0 6 2 104 3 0.0 6 3 105 3 0.0 6 3 106 2 0.0 6 2 107 2 0.0 6 2 108 8 0.0 6 8 109 2 0.0 6 2 110 4 0.0 6 4 111 3 0.0 6 3 112 1 0.0 6 1 115 3 0.0 6 3 116 1 0.0 6 1 117 3 0.0 6 3 119 1 0.0 6 1 120 3 0.0 6 3 121 2 0.0 6 2 122 8 0.0 6 8 123 7 0.0 6 7 124 2 0.0 6 2 125 2 0.0 6 2 126 3 0.0 6 3 127 1 0.0 6 1 129 5 0.0 6 5 130 3 0.0 6 3 131 1 0.0 6 1 132 5 0.0 6 5 135 2 0.0 6 2 136 3 0.0 6 3 137 1 0.0 6 1 138 4 0.0 6 4 139 4 0.0 6 4 140 1 0.0 6 1 141 4 0.0 6 4 142 2 0.0 6 2 143 1 0.0 6 1 144 1 0.0 6 1 145 1 0.0 6 1 146 5 0.0 6 5 147 2 0.0 6 2 148 4 0.0 6 4 149 2 0.0 6 2 150 2 0.0 6 2 151 1 0.0 6 1 152 1 0.0 6 1 153 5 0.0 6 5 154 2 0.0 6 1 1 155 3 0.0 6 3 157 1 0.0 6 1 158 3 0.0 6 3 161 2 0.0 6 2 162 2 0.0 6 2 164 1 0.0 6 1 165 4 0.0 6 4 166 1 0.0 6 1 167 2 0.0 6 2 168 8 0.0 6 8 169 1 0.0 6 1 171 2 0.0 6 2 172 3 0.0 6 3 173 4 0.0 6 4 178 1 0.0 6 1 180 1 0.0 6 1 183 1 0.0 6 1 186 1 0.0 6 1 187 1 0.0 6 1 188 2 0.0 6 2 189 1 0.0 6 1 190 1 0.0 6 1 192 2 0.0 6 2 193 1 0.0 6 1 194 5 0.0 6 5 195 2 0.0 6 2 196 3 0.0 6 3 197 1 0.0 6 1 198 3 0.0 6 3 199 1 0.0 6 1 200 1 0.0 6 1 201 3 0.0 6 3 202 2 0.0 6 2 206 2 0.0 6 2 208 1 0.0 6 1 210 1 0.0 6 1 212 2 0.0 6 2 215 1 0.0 6 1 216 2 0.0 6 2 217 1 0.0 6 1 219 3 0.0 6 3 220 1 0.0 6 1 222 1 0.0 6 1 224 2 0.0 6 2 226 1 0.0 6 1 227 1 0.0 6 1 228 2 0.0 6 2 229 2 0.0 6 2 230 2 0.0 6 2 236 2 0.0 6 2 238 3 0.0 6 3 254 1 0.0 6 1 279 1 0.0 6 1 281 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 71527 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1122.7 0 1 4 1 280.7 1 0 1 7 1 4.4 2 1 11 1 0.0 3 0 1 12 5 0.0 3 3 2 13 9 0.0 3 9 14 6 0.0 4 5 1 16 3 0.0 4 3 17 7 0.0 5 7 18 8 0.0 5 7 1 19 14 0.0 5 13 1 20 64 0.0 6 60 4 21 62 0.0 6 59 3 22 32 0.0 6 28 4 23 32 0.0 6 32 24 16 0.0 6 13 3 25 13 0.0 6 13 26 10 0.0 6 8 2 27 2 0.0 6 2 28 9 0.0 6 8 1 29 11 0.0 6 9 2 30 11 0.0 6 10 1 31 5 0.0 6 5 32 10 0.0 6 9 1 33 10 0.0 6 10 34 11 0.0 6 11 35 11 0.0 6 10 1 36 8 0.0 6 8 37 14 0.0 6 14 38 12 0.0 6 11 1 39 8 0.0 6 8 40 8 0.0 6 8 41 7 0.0 6 5 2 42 11 0.0 6 10 1 43 11 0.0 6 10 1 44 16 0.0 6 16 45 9 0.0 6 8 1 46 16 0.0 6 16 47 16 0.0 6 12 4 48 9 0.0 6 8 1 49 11 0.0 6 11 50 16 0.0 6 15 1 51 12 0.0 6 12 52 18 0.0 6 17 0 1 53 4 0.0 6 3 1 54 13 0.0 6 12 1 55 24 0.0 6 21 3 56 19 0.0 6 18 1 57 5 0.0 6 5 58 11 0.0 6 9 2 59 14 0.0 6 13 1 60 13 0.0 6 11 1 0 0 1 61 11 0.0 6 10 0 1 62 16 0.0 6 14 2 63 5 0.0 6 4 1 64 13 0.0 6 13 65 12 0.0 6 12 66 22 0.0 6 18 4 67 14 0.0 6 12 2 68 34 0.0 6 33 1 69 126 0.0 6 126 70 33 0.0 6 30 3 71 55 0.0 6 55 72 37 0.0 6 35 2 73 29 0.0 6 29 74 18 0.0 6 18 75 12 0.0 6 11 1 76 26 0.0 6 24 2 77 24 0.0 6 22 2 78 25 0.0 6 22 3 79 29 0.0 6 27 2 80 19 0.0 6 18 1 81 26 0.0 6 25 0 1 82 29 0.0 6 25 4 83 37 0.0 6 36 1 84 60 0.0 6 59 1 85 53 0.0 6 51 2 86 38 0.0 6 36 2 87 43 0.0 6 39 4 88 22 0.0 6 22 89 38 0.0 6 37 1 90 37 0.0 6 35 2 91 45 0.0 6 42 2 1 92 34 0.0 6 32 2 93 51 0.0 6 49 2 94 81 0.0 6 76 4 0 0 1 95 35 0.0 6 34 1 96 49 0.0 6 47 2 97 56 0.0 6 52 4 98 51 0.0 6 47 4 99 45 0.0 6 41 4 100 50 0.0 6 45 4 1 101 50 0.0 6 47 1 2 102 67 0.0 6 62 4 0 0 0 1 103 78 0.0 6 71 6 1 104 71 0.0 6 69 2 105 74 0.0 6 70 3 1 106 87 0.0 6 84 2 0 0 0 1 107 57 0.0 6 53 2 1 0 1 108 50 0.0 6 46 4 109 44 0.0 6 44 110 73 0.0 6 69 3 1 111 38 0.0 6 36 2 112 66 0.0 6 65 1 113 48 0.0 6 46 2 114 89 0.0 6 85 3 1 115 79 0.0 6 77 2 116 72 0.0 6 67 5 117 56 0.0 6 56 118 70 0.0 6 66 3 1 119 88 0.0 6 82 5 1 120 140 0.0 6 136 3 1 121 84 0.0 6 79 5 122 79 0.0 6 78 1 123 89 0.0 6 84 4 0 0 1 124 89 0.0 6 88 1 125 80 0.0 6 77 3 126 75 0.0 6 74 1 127 106 0.0 6 104 1 0 1 128 98 0.0 6 96 2 129 129 0.0 6 126 3 130 101 0.0 6 97 4 131 138 0.0 6 136 2 132 158 0.0 6 153 5 133 114 0.0 6 111 2 1 134 201 0.0 6 195 5 1 135 309 0.0 6 301 8 136 263 0.0 6 257 5 1 137 272 0.0 6 269 3 138 232 0.0 6 229 3 139 312 0.0 6 305 7 140 380 0.0 6 371 8 0 0 0 1 141 298 0.0 6 295 3 142 417 0.0 6 408 8 1 143 403 0.0 6 395 8 144 489 0.0 6 481 8 145 370 0.0 6 362 7 0 0 1 146 362 0.0 6 356 6 147 291 0.0 6 287 2 0 0 1 1 148 399 0.0 6 392 6 1 149 471 0.0 6 464 7 150 619 0.0 6 602 16 1 151 436 0.0 6 427 8 1 152 764 0.0 6 746 16 2 153 485 0.0 6 476 9 154 600 0.0 6 589 9 0 0 1 0 1 155 566 0.0 6 560 5 1 156 444 0.0 6 434 8 0 0 2 157 622 0.0 6 608 14 158 638 0.0 6 622 14 2 159 503 0.0 6 491 12 160 669 0.0 6 656 13 161 449 0.0 6 438 10 0 0 1 162 402 0.0 6 393 9 163 529 0.0 6 513 15 1 164 486 0.0 6 472 11 2 0 0 1 165 546 0.0 6 532 14 166 571 0.0 6 562 9 167 463 0.0 6 450 13 168 701 0.0 6 688 13 169 361 0.0 6 356 3 1 1 170 563 0.0 6 550 12 0 0 1 171 623 0.0 6 609 13 0 1 172 344 0.0 6 335 8 1 173 549 0.0 6 539 8 1 1 174 716 0.0 6 692 23 0 0 1 175 1156 0.0 6 1124 31 1 176 715 0.0 6 697 16 0 0 1 0 1 177 766 0.0 6 752 14 178 637 0.0 6 620 15 0 1 1 179 645 0.0 6 626 17 2 180 711 0.0 6 695 16 181 642 0.0 6 629 10 1 0 2 182 314 0.0 6 299 13 1 0 0 1 183 673 0.0 6 660 12 1 184 235 0.0 6 232 3 185 417 0.0 6 406 9 1 1 186 1320 0.0 6 1291 27 2 187 1088 0.0 6 1043 43 0 0 2 188 1251 0.0 6 1213 36 1 0 1 189 1088 0.0 6 1051 35 1 1 190 1501 0.0 6 1467 31 2 0 1 191 1293 0.0 6 1257 35 1 192 854 0.0 6 828 25 0 0 1 193 771 0.0 6 753 16 2 194 840 0.0 6 819 18 2 0 1 195 1323 0.0 6 1274 49 196 2042 0.0 6 1999 42 1 197 461 0.0 6 439 20 2 198 1880 0.0 6 1826 53 0 0 1 199 1989 0.0 6 1932 57 200 880 0.0 6 848 29 1 1 1 201 1141 0.0 6 1116 25 202 911 0.0 6 888 19 1 1 2 203 694 0.0 6 671 22 1 204 1054 0.0 6 1026 22 3 0 2 1 205 540 0.0 6 525 14 0 1 206 487 0.0 6 476 11 207 399 0.0 6 388 11 208 781 0.0 6 761 20 209 264 0.0 6 245 16 2 1 210 448 0.0 6 433 13 1 0 1 211 627 0.0 6 614 12 0 0 1 212 524 0.0 6 513 11 213 161 0.0 6 155 6 214 468 0.0 6 456 12 215 644 0.0 6 628 16 216 949 0.0 6 919 29 1 217 674 0.0 6 650 23 1 218 535 0.0 6 522 11 1 1 219 489 0.0 6 469 19 0 1 220 273 0.0 6 268 5 221 430 0.0 6 421 9 222 303 0.0 6 295 8 223 405 0.0 6 395 10 224 460 0.0 6 449 8 3 225 407 0.0 6 393 10 3 0 1 226 587 0.0 6 571 14 1 0 1 227 602 0.0 6 583 17 2 228 455 0.0 6 435 17 2 0 1 229 692 0.0 6 668 23 0 0 1 230 1440 0.0 6 1390 46 1 1 2 231 424 0.0 6 406 18 232 618 0.0 6 601 17 233 310 0.0 6 304 6 234 560 0.0 6 544 14 1 0 1 235 208 0.0 6 199 8 1 236 193 0.0 6 188 5 237 159 0.0 6 152 7 238 82 0.0 6 80 2 239 32 0.0 6 30 1 1 240 75 0.0 6 71 4 241 129 0.0 6 125 4 242 132 0.0 6 126 5 0 1 243 149 0.0 6 142 6 0 0 1 244 101 0.0 6 100 1 245 163 0.0 6 157 6 246 132 0.0 6 130 2 247 90 0.0 6 85 5 248 56 0.0 6 53 3 249 126 0.0 6 123 3 250 69 0.0 6 68 1 251 150 0.0 6 143 6 1 252 56 0.0 6 53 3 253 74 0.0 6 72 2 254 26 0.0 6 24 2 255 23 0.0 6 23 256 17 0.0 6 16 1 257 2 0.0 6 2 259 2 0.0 6 2 260 2 0.0 6 2 268 2 0.0 6 2 279 3 0.0 6 3 280 20 0.0 6 20 281 37 0.0 6 34 2 0 0 1 282 4 0.0 6 4 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 288 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 55 1 0.0 6 1 66 1 0.0 6 1 69 1 0.0 6 1 70 1 0.0 6 1 71 4 0.0 6 4 72 6 0.0 6 6 73 2 0.0 6 2 74 1 0.0 6 1 75 4 0.0 6 4 76 2 0.0 6 2 77 6 0.0 6 6 78 1 0.0 6 1 79 1 0.0 6 1 80 5 0.0 6 5 81 3 0.0 6 3 82 3 0.0 6 3 83 4 0.0 6 4 84 3 0.0 6 2 0 0 0 1 85 1 0.0 6 1 86 3 0.0 6 3 87 1 0.0 6 1 88 2 0.0 6 2 89 2 0.0 6 2 90 2 0.0 6 2 91 5 0.0 6 5 93 4 0.0 6 4 95 1 0.0 6 1 96 4 0.0 6 4 98 1 0.0 6 1 99 4 0.0 6 4 100 4 0.0 6 4 102 3 0.0 6 3 103 5 0.0 6 5 104 2 0.0 6 2 105 3 0.0 6 3 106 2 0.0 6 2 107 5 0.0 6 5 108 7 0.0 6 7 109 3 0.0 6 3 110 3 0.0 6 3 111 4 0.0 6 4 112 2 0.0 6 2 113 2 0.0 6 2 114 3 0.0 6 3 115 4 0.0 6 4 116 4 0.0 6 4 117 4 0.0 6 4 118 2 0.0 6 2 120 3 0.0 6 3 122 4 0.0 6 4 123 3 0.0 6 3 124 3 0.0 6 2 0 0 0 1 125 3 0.0 6 3 127 1 0.0 6 1 128 1 0.0 6 1 129 4 0.0 6 4 130 5 0.0 6 5 131 3 0.0 6 3 132 5 0.0 6 5 133 4 0.0 6 4 134 1 0.0 6 1 135 4 0.0 6 4 137 2 0.0 6 2 138 5 0.0 6 5 139 1 0.0 6 1 140 1 0.0 6 1 141 2 0.0 6 2 142 3 0.0 6 3 143 1 0.0 6 1 144 2 0.0 6 2 145 3 0.0 6 3 146 3 0.0 6 3 148 3 0.0 6 3 149 4 0.0 6 4 150 3 0.0 6 3 151 2 0.0 6 2 152 2 0.0 6 2 153 4 0.0 6 4 154 2 0.0 6 2 155 1 0.0 6 1 156 4 0.0 6 4 157 4 0.0 6 4 158 1 0.0 6 1 159 1 0.0 6 1 160 1 0.0 6 1 161 2 0.0 6 2 162 1 0.0 6 1 163 1 0.0 6 1 164 1 0.0 6 1 165 1 0.0 6 1 167 2 0.0 6 2 169 2 0.0 6 2 170 3 0.0 6 3 171 2 0.0 6 2 172 4 0.0 6 4 173 1 0.0 6 1 174 2 0.0 6 1 1 175 2 0.0 6 2 179 2 0.0 6 2 180 1 0.0 6 1 182 1 0.0 6 1 183 3 0.0 6 3 184 3 0.0 6 3 186 1 0.0 6 1 188 1 0.0 6 1 192 1 0.0 6 1 193 1 0.0 6 1 195 2 0.0 6 2 196 2 0.0 6 2 198 1 0.0 6 1 204 1 0.0 6 1 207 1 0.0 6 1 208 1 0.0 6 1 218 1 0.0 6 1 239 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_20_143_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_20_370_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_20_143_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_20_370_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.31 s (51461 µs/read; 0.00 M reads/minute). === Summary === Total read pairs processed: 6 Read 1 with adapter: 6 (100.0%) Read 2 with adapter: 6 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 0 (0.0%) Pairs written (passing filters): 6 (100.0%) Total basepairs processed: 2,252 bp Read 1: 1,350 bp Read 2: 902 bp Total written (filtered): 373 bp (16.6%) Read 1: 181 bp Read 2: 192 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 6 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 86 1 0.0 6 1 164 1 0.0 6 1 167 1 0.0 6 1 199 1 0.0 6 1 272 1 0.0 6 1 281 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 0 times === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 6 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 17 1 0.0 5 1 89 1 0.0 6 1 133 1 0.0 6 1 139 1 0.0 6 1 143 1 0.0 6 1 189 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 0 times cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_01_132_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_01_359_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_01_132_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_01_359_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_02_133_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_02_360_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_02_133_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_02_360_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_03_134_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_03_361_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_03_134_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_03_361_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_04_135_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_04_362_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_04_135_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_04_362_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_05_136_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_05_363_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_05_136_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_05_363_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_06_137_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_06_364_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_06_137_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_06_364_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_15_138_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_15_365_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_15_138_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_15_365_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_16_139_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_16_366_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_16_139_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_16_366_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_17_140_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_17_367_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_17_140_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_17_367_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_18_141_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_18_368_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_18_141_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_18_368_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_19_142_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_19_369_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_19_142_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_19_369_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_20_143_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_12-Rev_20_370_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_20_143_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_12-Rev_20_370_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_07_144_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_07_371_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_07_144_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_07_371_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.30 s (60 µs/read; 1.00 M reads/minute). === Summary === Total read pairs processed: 54,959 Read 1 with adapter: 54,956 (100.0%) Read 2 with adapter: 54,909 (99.9%) == Read fate breakdown == Pairs that were too short: 2 (0.0%) Pairs discarded as untrimmed: 53 (0.1%) Pairs written (passing filters): 54,904 (99.9%) Total basepairs processed: 27,292,070 bp Read 1: 15,605,376 bp Read 2: 11,686,694 bp Total written (filtered): 3,581,128 bp (13.1%) Read 1: 1,819,508 bp Read 2: 1,761,620 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 54795 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 858.7 0 0 1 5 2 53.7 1 2 6 1 13.4 1 0 0 1 7 1 3.4 2 1 8 1 0.8 2 1 9 3 0.2 2 3 13 1 0.0 3 1 19 1 0.0 5 1 20 10 0.0 6 9 1 21 6 0.0 6 5 1 22 3 0.0 6 3 23 1 0.0 6 1 24 2 0.0 6 2 25 6 0.0 6 6 26 4 0.0 6 4 27 1 0.0 6 1 28 2 0.0 6 2 29 3 0.0 6 3 30 2 0.0 6 2 31 1 0.0 6 0 1 32 2 0.0 6 2 33 2 0.0 6 2 34 1 0.0 6 1 36 1 0.0 6 1 37 1 0.0 6 1 38 2 0.0 6 2 39 3 0.0 6 3 40 3 0.0 6 2 1 41 2 0.0 6 2 42 1 0.0 6 1 43 2 0.0 6 2 44 5 0.0 6 5 45 1 0.0 6 1 46 4 0.0 6 3 1 47 4 0.0 6 4 48 2 0.0 6 2 49 1 0.0 6 1 50 2 0.0 6 2 51 1 0.0 6 1 52 1 0.0 6 0 0 1 54 1 0.0 6 1 55 2 0.0 6 2 56 4 0.0 6 4 57 3 0.0 6 2 1 59 1 0.0 6 1 60 3 0.0 6 3 62 3 0.0 6 3 63 2 0.0 6 2 64 7 0.0 6 7 66 2 0.0 6 1 0 0 1 67 3 0.0 6 3 69 1 0.0 6 1 70 1 0.0 6 1 71 4 0.0 6 4 72 3 0.0 6 3 73 1 0.0 6 1 74 1 0.0 6 1 77 4 0.0 6 4 78 4 0.0 6 4 79 3 0.0 6 3 80 2 0.0 6 2 82 2 0.0 6 2 83 3 0.0 6 3 85 4 0.0 6 4 86 7 0.0 6 7 87 1 0.0 6 1 88 1 0.0 6 1 89 2 0.0 6 2 90 2 0.0 6 1 1 91 4 0.0 6 4 92 4 0.0 6 4 93 4 0.0 6 4 94 4 0.0 6 4 95 1 0.0 6 1 96 2 0.0 6 2 97 1 0.0 6 1 98 6 0.0 6 6 100 4 0.0 6 3 1 101 2 0.0 6 2 102 8 0.0 6 7 0 0 0 0 0 1 103 5 0.0 6 5 104 6 0.0 6 6 105 5 0.0 6 4 1 106 4 0.0 6 3 1 107 6 0.0 6 4 2 108 11 0.0 6 11 109 3 0.0 6 3 110 2 0.0 6 2 111 11 0.0 6 10 1 112 11 0.0 6 8 2 1 113 13 0.0 6 11 2 114 7 0.0 6 7 115 4 0.0 6 3 1 116 5 0.0 6 4 0 0 1 117 6 0.0 6 6 118 6 0.0 6 4 1 1 119 11 0.0 6 11 120 5 0.0 6 5 121 11 0.0 6 9 2 122 14 0.0 6 13 1 123 13 0.0 6 13 124 8 0.0 6 8 125 3 0.0 6 2 1 126 15 0.0 6 13 2 127 7 0.0 6 6 1 128 23 0.0 6 21 2 129 12 0.0 6 11 1 130 15 0.0 6 13 2 131 10 0.0 6 9 1 132 11 0.0 6 11 133 10 0.0 6 10 134 15 0.0 6 15 135 17 0.0 6 15 2 136 17 0.0 6 16 1 137 14 0.0 6 13 1 138 20 0.0 6 19 0 1 139 21 0.0 6 20 0 1 140 23 0.0 6 21 2 141 28 0.0 6 27 1 142 33 0.0 6 33 143 100 0.0 6 100 144 70 0.0 6 69 1 145 126 0.0 6 125 1 146 69 0.0 6 69 147 116 0.0 6 116 148 96 0.0 6 95 1 149 69 0.0 6 68 1 150 78 0.0 6 78 151 114 0.0 6 114 152 101 0.0 6 101 153 100 0.0 6 98 2 154 76 0.0 6 76 155 75 0.0 6 74 1 156 74 0.0 6 73 0 1 157 56 0.0 6 55 1 158 49 0.0 6 49 159 64 0.0 6 63 1 160 72 0.0 6 70 2 161 79 0.0 6 79 162 81 0.0 6 81 163 73 0.0 6 73 164 59 0.0 6 57 2 165 54 0.0 6 53 1 166 79 0.0 6 76 3 167 61 0.0 6 59 2 168 40 0.0 6 40 169 63 0.0 6 62 1 170 55 0.0 6 55 171 65 0.0 6 64 1 172 100 0.0 6 100 173 106 0.0 6 106 174 90 0.0 6 89 1 175 83 0.0 6 83 176 80 0.0 6 78 2 177 83 0.0 6 83 178 67 0.0 6 66 1 179 63 0.0 6 63 180 82 0.0 6 82 181 164 0.0 6 161 3 182 93 0.0 6 93 183 81 0.0 6 78 3 184 86 0.0 6 83 3 185 109 0.0 6 104 5 186 131 0.0 6 131 187 136 0.0 6 135 0 1 188 144 0.0 6 140 4 189 165 0.0 6 162 3 190 105 0.0 6 104 1 191 135 0.0 6 133 2 192 168 0.0 6 165 2 1 193 175 0.0 6 167 8 194 192 0.0 6 187 4 1 195 260 0.0 6 257 3 196 375 0.0 6 367 7 1 197 211 0.0 6 208 3 198 222 0.0 6 215 6 1 199 179 0.0 6 172 7 200 160 0.0 6 157 3 201 197 0.0 6 191 6 202 185 0.0 6 183 2 203 193 0.0 6 188 5 204 221 0.0 6 219 1 1 205 298 0.0 6 292 6 206 252 0.0 6 247 3 2 207 285 0.0 6 279 6 208 235 0.0 6 228 5 1 0 1 209 198 0.0 6 191 7 210 216 0.0 6 212 4 211 178 0.0 6 175 3 212 148 0.0 6 146 2 213 196 0.0 6 192 4 214 227 0.0 6 222 4 1 215 145 0.0 6 140 4 1 216 186 0.0 6 183 3 217 276 0.0 6 270 6 218 233 0.0 6 226 7 219 191 0.0 6 187 4 220 175 0.0 6 172 3 221 159 0.0 6 152 6 1 222 272 0.0 6 269 3 223 150 0.0 6 146 3 1 224 196 0.0 6 195 1 225 229 0.0 6 223 6 226 315 0.0 6 311 3 1 227 241 0.0 6 237 4 228 220 0.0 6 216 3 1 229 214 0.0 6 211 3 230 231 0.0 6 221 9 1 231 175 0.0 6 167 8 232 140 0.0 6 138 2 233 108 0.0 6 105 3 234 139 0.0 6 138 1 235 150 0.0 6 148 2 236 130 0.0 6 125 5 237 214 0.0 6 205 9 238 186 0.0 6 182 4 239 241 0.0 6 236 5 240 286 0.0 6 279 7 241 504 0.0 6 492 11 0 0 1 242 402 0.0 6 394 7 1 243 418 0.0 6 412 5 1 244 265 0.0 6 255 10 245 407 0.0 6 399 8 246 178 0.0 6 170 8 247 252 0.0 6 246 5 1 248 141 0.0 6 131 8 1 0 1 249 390 0.0 6 386 4 250 234 0.0 6 232 2 251 280 0.0 6 276 4 252 248 0.0 6 243 5 253 161 0.0 6 154 7 254 395 0.0 6 387 8 255 246 0.0 6 238 8 256 225 0.0 6 220 4 0 1 257 244 0.0 6 237 7 258 321 0.0 6 313 8 259 363 0.0 6 344 19 260 342 0.0 6 337 5 261 158 0.0 6 151 7 262 347 0.0 6 336 11 263 541 0.0 6 535 5 1 264 341 0.0 6 328 12 0 0 1 265 442 0.0 6 435 7 266 189 0.0 6 187 2 267 459 0.0 6 444 14 1 268 489 0.0 6 471 18 269 465 0.0 6 455 10 270 200 0.0 6 190 10 271 413 0.0 6 400 12 0 1 272 1423 0.0 6 1401 21 1 273 730 0.0 6 705 25 274 1426 0.0 6 1400 26 275 406 0.0 6 390 15 1 276 1348 0.0 6 1308 40 277 8670 0.0 6 8506 160 4 278 15303 0.0 6 14956 336 9 1 0 1 279 233 0.0 6 212 19 2 280 32 0.0 6 26 2 1 3 281 3 0.0 6 2 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 161 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 1 0.0 6 1 64 14 0.0 6 14 74 1 0.0 6 1 75 1 0.0 6 1 76 1 0.0 6 1 77 3 0.0 6 3 78 1 0.0 6 1 79 1 0.0 6 1 80 2 0.0 6 2 81 6 0.0 6 6 82 3 0.0 6 3 83 3 0.0 6 3 85 3 0.0 6 3 86 3 0.0 6 3 87 1 0.0 6 1 89 1 0.0 6 1 91 1 0.0 6 1 92 1 0.0 6 1 95 4 0.0 6 4 97 1 0.0 6 1 98 1 0.0 6 1 99 1 0.0 6 1 101 1 0.0 6 1 102 1 0.0 6 1 106 2 0.0 6 2 107 4 0.0 6 4 109 1 0.0 6 1 110 1 0.0 6 1 111 2 0.0 6 2 116 1 0.0 6 1 117 1 0.0 6 1 118 1 0.0 6 1 120 2 0.0 6 2 121 1 0.0 6 1 123 1 0.0 6 1 124 2 0.0 6 2 125 1 0.0 6 1 126 1 0.0 6 1 127 1 0.0 6 1 129 2 0.0 6 2 131 1 0.0 6 1 133 1 0.0 6 1 134 3 0.0 6 3 135 2 0.0 6 2 136 1 0.0 6 1 137 1 0.0 6 1 138 2 0.0 6 2 139 2 0.0 6 2 141 1 0.0 6 1 143 1 0.0 6 1 144 1 0.0 6 1 145 1 0.0 6 1 147 1 0.0 6 1 150 1 0.0 6 1 151 2 0.0 6 2 152 2 0.0 6 2 157 1 0.0 6 1 158 1 0.0 6 1 159 1 0.0 6 1 160 3 0.0 6 3 161 2 0.0 6 2 162 1 0.0 6 1 166 1 0.0 6 1 167 1 0.0 6 1 168 1 0.0 6 1 169 2 0.0 6 2 171 1 0.0 6 1 172 3 0.0 6 3 173 1 0.0 6 1 174 2 0.0 6 2 175 1 0.0 6 1 176 1 0.0 6 1 177 1 0.0 6 1 179 1 0.0 6 1 180 1 0.0 6 1 181 1 0.0 6 1 183 2 0.0 6 2 184 1 0.0 6 1 185 1 0.0 6 1 188 2 0.0 6 2 189 1 0.0 6 1 192 1 0.0 6 1 194 2 0.0 6 2 197 1 0.0 6 1 201 2 0.0 6 2 204 1 0.0 6 1 205 1 0.0 6 1 208 1 0.0 6 1 209 3 0.0 6 3 210 1 0.0 6 1 212 1 0.0 6 1 214 1 0.0 6 1 220 1 0.0 6 1 222 1 0.0 6 1 230 1 0.0 6 1 231 1 0.0 6 1 232 1 0.0 6 1 234 1 0.0 6 1 242 1 0.0 6 1 261 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 54765 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 858.7 0 0 2 6 1 13.4 1 1 9 1 0.2 2 0 1 10 3 0.1 3 3 11 3 0.0 3 3 12 2 0.0 3 1 1 13 6 0.0 3 5 1 14 5 0.0 4 4 1 16 1 0.0 4 1 17 13 0.0 5 12 1 18 14 0.0 5 12 2 19 5 0.0 5 5 20 47 0.0 6 44 3 21 45 0.0 6 43 2 22 19 0.0 6 18 1 23 19 0.0 6 16 3 24 24 0.0 6 19 4 1 25 14 0.0 6 11 3 26 15 0.0 6 13 2 27 11 0.0 6 11 28 14 0.0 6 12 1 0 0 1 29 18 0.0 6 18 30 11 0.0 6 10 1 31 8 0.0 6 7 1 32 10 0.0 6 7 3 33 12 0.0 6 11 1 34 7 0.0 6 6 1 35 5 0.0 6 4 1 36 7 0.0 6 7 37 7 0.0 6 6 1 38 7 0.0 6 7 39 9 0.0 6 7 2 40 4 0.0 6 4 41 6 0.0 6 6 42 11 0.0 6 11 43 11 0.0 6 11 44 15 0.0 6 13 1 0 1 45 6 0.0 6 4 2 46 21 0.0 6 20 1 47 19 0.0 6 17 2 48 9 0.0 6 7 2 49 16 0.0 6 15 1 50 14 0.0 6 13 0 1 51 16 0.0 6 13 3 52 27 0.0 6 23 4 53 9 0.0 6 6 3 54 21 0.0 6 19 2 55 11 0.0 6 10 1 56 15 0.0 6 14 1 57 17 0.0 6 16 1 58 14 0.0 6 14 59 10 0.0 6 8 1 1 60 18 0.0 6 15 2 0 0 1 61 13 0.0 6 12 1 62 9 0.0 6 7 2 63 6 0.0 6 5 1 64 12 0.0 6 11 1 65 14 0.0 6 10 3 0 0 1 66 27 0.0 6 25 1 1 67 93 0.0 6 87 6 68 30 0.0 6 26 3 0 0 1 69 39 0.0 6 35 3 1 70 20 0.0 6 18 2 71 28 0.0 6 27 0 0 1 72 18 0.0 6 17 1 73 12 0.0 6 11 1 74 17 0.0 6 17 75 13 0.0 6 9 4 76 18 0.0 6 17 1 77 14 0.0 6 12 2 78 23 0.0 6 21 2 79 12 0.0 6 12 80 17 0.0 6 15 1 1 81 17 0.0 6 16 1 82 34 0.0 6 29 5 83 49 0.0 6 40 8 1 84 31 0.0 6 26 4 1 85 18 0.0 6 15 2 1 86 28 0.0 6 26 2 87 43 0.0 6 34 9 88 25 0.0 6 21 3 1 89 26 0.0 6 22 2 1 1 90 30 0.0 6 28 2 91 20 0.0 6 19 1 92 17 0.0 6 14 3 93 50 0.0 6 43 7 94 50 0.0 6 47 3 95 37 0.0 6 32 5 96 44 0.0 6 37 4 1 1 1 97 37 0.0 6 32 4 1 98 40 0.0 6 37 3 99 43 0.0 6 42 1 100 49 0.0 6 44 4 0 1 101 43 0.0 6 42 1 102 54 0.0 6 50 4 103 85 0.0 6 78 7 104 87 0.0 6 84 2 1 105 76 0.0 6 69 5 0 1 0 0 1 106 46 0.0 6 44 1 0 0 1 107 46 0.0 6 44 2 108 33 0.0 6 30 3 109 50 0.0 6 47 1 2 110 59 0.0 6 52 7 111 58 0.0 6 55 3 112 61 0.0 6 57 2 2 113 44 0.0 6 38 6 114 71 0.0 6 62 8 0 1 115 54 0.0 6 50 4 116 63 0.0 6 56 6 0 1 117 56 0.0 6 55 0 0 0 1 118 64 0.0 6 62 2 119 71 0.0 6 70 1 120 79 0.0 6 76 3 121 48 0.0 6 45 3 122 63 0.0 6 60 2 0 1 123 62 0.0 6 62 124 85 0.0 6 82 3 125 92 0.0 6 88 3 0 0 1 126 70 0.0 6 69 1 127 92 0.0 6 83 7 1 1 128 87 0.0 6 84 3 129 82 0.0 6 77 4 0 0 1 130 67 0.0 6 66 1 131 113 0.0 6 104 8 0 1 132 156 0.0 6 153 3 133 130 0.0 6 120 9 1 134 169 0.0 6 159 8 0 1 1 135 262 0.0 6 256 5 0 0 1 136 136 0.0 6 133 3 137 173 0.0 6 165 6 1 0 1 138 116 0.0 6 111 4 0 0 0 0 1 139 169 0.0 6 166 2 0 0 0 0 1 140 238 0.0 6 232 6 141 281 0.0 6 270 9 2 142 318 0.0 6 307 11 143 334 0.0 6 319 14 1 144 385 0.0 6 375 9 1 145 268 0.0 6 257 9 1 0 1 146 276 0.0 6 270 6 147 228 0.0 6 217 8 2 0 1 148 288 0.0 6 279 8 0 0 0 0 1 149 427 0.0 6 415 9 0 2 0 0 1 150 694 0.0 6 669 22 1 0 1 0 1 151 283 0.0 6 275 7 0 0 0 0 1 152 592 0.0 6 579 11 1 0 1 153 383 0.0 6 372 10 0 0 0 0 1 154 327 0.0 6 317 10 155 341 0.0 6 331 10 156 288 0.0 6 279 8 0 0 0 1 157 446 0.0 6 437 9 158 457 0.0 6 451 6 159 295 0.0 6 289 6 160 402 0.0 6 395 7 161 260 0.0 6 254 3 3 162 251 0.0 6 242 8 1 163 400 0.0 6 387 13 164 398 0.0 6 386 11 1 165 406 0.0 6 388 16 1 0 1 166 461 0.0 6 447 12 0 0 1 0 1 167 311 0.0 6 300 11 168 498 0.0 6 486 10 0 1 0 0 1 169 218 0.0 6 212 6 170 280 0.0 6 271 9 171 487 0.0 6 470 17 172 364 0.0 6 350 12 1 0 1 173 527 0.0 6 506 20 0 0 1 174 623 0.0 6 598 25 175 989 0.0 6 965 22 2 176 636 0.0 6 608 25 1 1 1 177 621 0.0 6 600 19 1 1 178 458 0.0 6 447 9 1 1 179 541 0.0 6 530 11 180 416 0.0 6 406 9 0 1 181 530 0.0 6 517 13 182 210 0.0 6 202 7 1 183 601 0.0 6 583 16 2 184 519 0.0 6 503 14 1 1 185 690 0.0 6 664 25 0 1 186 1160 0.0 6 1119 38 2 0 1 187 998 0.0 6 975 19 3 0 1 188 919 0.0 6 891 26 1 0 1 189 847 0.0 6 812 33 1 0 1 190 1228 0.0 6 1199 25 3 0 1 191 1211 0.0 6 1172 38 1 192 693 0.0 6 678 15 193 426 0.0 6 404 19 1 2 194 886 0.0 6 860 24 2 195 1263 0.0 6 1216 46 0 1 196 2266 0.0 6 2195 68 1 0 1 0 1 197 757 0.0 6 729 27 1 198 1405 0.0 6 1366 38 0 1 199 1964 0.0 6 1914 46 1 0 3 200 811 0.0 6 771 37 1 1 1 201 1177 0.0 6 1139 36 0 0 2 202 510 0.0 6 497 11 1 0 1 203 447 0.0 6 435 8 3 0 0 1 204 871 0.0 6 853 18 205 297 0.0 6 293 4 206 280 0.0 6 270 9 1 207 134 0.0 6 126 5 2 0 1 208 340 0.0 6 324 15 1 209 272 0.0 6 268 3 0 1 210 285 0.0 6 272 12 1 211 309 0.0 6 300 9 212 527 0.0 6 515 12 213 241 0.0 6 231 10 214 943 0.0 6 921 20 1 1 215 469 0.0 6 454 13 2 216 325 0.0 6 312 11 1 0 1 217 406 0.0 6 391 13 1 0 1 218 212 0.0 6 207 5 219 338 0.0 6 329 8 0 0 1 220 113 0.0 6 106 7 221 391 0.0 6 378 12 0 0 1 222 331 0.0 6 322 8 1 223 539 0.0 6 524 14 1 224 304 0.0 6 288 15 1 225 380 0.0 6 375 5 226 232 0.0 6 227 5 227 246 0.0 6 243 3 228 140 0.0 6 137 3 229 312 0.0 6 306 4 2 230 164 0.0 6 161 3 231 263 0.0 6 260 3 232 304 0.0 6 299 5 233 222 0.0 6 212 10 234 113 0.0 6 113 235 72 0.0 6 70 2 236 27 0.0 6 27 237 28 0.0 6 26 2 238 28 0.0 6 27 1 239 18 0.0 6 18 240 81 0.0 6 80 1 241 119 0.0 6 114 5 242 157 0.0 6 155 2 243 89 0.0 6 82 6 0 0 0 0 1 244 97 0.0 6 96 0 1 245 73 0.0 6 72 1 246 89 0.0 6 87 2 247 31 0.0 6 28 3 248 69 0.0 6 68 1 249 16 0.0 6 16 250 15 0.0 6 15 251 26 0.0 6 25 1 252 1 0.0 6 1 253 2 0.0 6 1 1 257 1 0.0 6 0 1 277 1 0.0 6 1 278 15 0.0 6 15 279 43 0.0 6 39 1 0 2 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 144 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 6 0.0 6 6 72 2 0.0 6 2 73 1 0.0 6 1 74 5 0.0 6 5 75 1 0.0 6 1 76 8 0.0 6 8 77 2 0.0 6 2 78 3 0.0 6 3 79 2 0.0 6 2 80 4 0.0 6 4 81 2 0.0 6 2 82 2 0.0 6 2 83 2 0.0 6 2 87 1 0.0 6 1 89 3 0.0 6 3 90 1 0.0 6 1 91 2 0.0 6 2 92 2 0.0 6 2 95 1 0.0 6 1 98 1 0.0 6 1 101 3 0.0 6 3 102 1 0.0 6 1 103 3 0.0 6 3 104 1 0.0 6 1 105 2 0.0 6 2 106 1 0.0 6 1 107 1 0.0 6 1 108 1 0.0 6 1 110 4 0.0 6 4 111 1 0.0 6 1 114 3 0.0 6 3 115 4 0.0 6 4 116 1 0.0 6 1 117 1 0.0 6 1 118 1 0.0 6 1 120 1 0.0 6 1 121 3 0.0 6 3 123 1 0.0 6 1 125 1 0.0 6 1 128 2 0.0 6 2 133 2 0.0 6 2 134 2 0.0 6 2 137 1 0.0 6 1 140 1 0.0 6 1 142 1 0.0 6 1 143 2 0.0 6 2 144 3 0.0 6 3 145 3 0.0 6 3 147 2 0.0 6 2 150 1 0.0 6 1 153 2 0.0 6 2 154 1 0.0 6 1 156 1 0.0 6 1 158 2 0.0 6 2 160 1 0.0 6 1 161 2 0.0 6 2 163 1 0.0 6 1 164 3 0.0 6 3 165 3 0.0 6 3 167 1 0.0 6 1 168 3 0.0 6 3 170 3 0.0 6 3 175 1 0.0 6 1 176 1 0.0 6 1 179 1 0.0 6 1 180 1 0.0 6 1 181 1 0.0 6 1 182 1 0.0 6 1 186 1 0.0 6 1 188 1 0.0 6 1 190 3 0.0 6 3 193 1 0.0 6 1 196 1 0.0 6 1 206 2 0.0 6 2 210 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_08_145_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_08_372_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_08_145_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_08_372_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.20 s (47 µs/read; 1.28 M reads/minute). === Summary === Total read pairs processed: 68,511 Read 1 with adapter: 68,508 (100.0%) Read 2 with adapter: 68,511 (100.0%) == Read fate breakdown == Pairs that were too short: 20 (0.0%) Pairs discarded as untrimmed: 3 (0.0%) Pairs written (passing filters): 68,488 (100.0%) Total basepairs processed: 29,509,437 bp Read 1: 16,466,375 bp Read 2: 13,043,062 bp Total written (filtered): 4,483,775 bp (15.2%) Read 1: 2,322,188 bp Read 2: 2,161,587 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 67358 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 2 267.6 1 1 1 5 2 66.9 1 2 6 4 16.7 1 1 0 3 9 5 0.3 2 3 2 11 1 0.0 3 1 16 2 0.0 4 1 1 17 2 0.0 5 0 0 1 1 18 2 0.0 5 1 1 19 3 0.0 5 3 20 57 0.0 6 56 1 21 33 0.0 6 33 22 26 0.0 6 26 23 26 0.0 6 24 2 24 18 0.0 6 18 25 18 0.0 6 18 26 12 0.0 6 12 27 8 0.0 6 8 28 3 0.0 6 3 29 5 0.0 6 5 30 2 0.0 6 1 1 31 5 0.0 6 5 32 5 0.0 6 5 33 9 0.0 6 8 1 34 5 0.0 6 4 0 0 1 35 1 0.0 6 1 36 3 0.0 6 3 37 4 0.0 6 3 1 38 2 0.0 6 2 39 7 0.0 6 7 40 9 0.0 6 9 41 8 0.0 6 8 42 2 0.0 6 2 43 5 0.0 6 5 44 4 0.0 6 4 45 4 0.0 6 4 46 8 0.0 6 8 47 2 0.0 6 2 48 5 0.0 6 5 49 5 0.0 6 5 50 3 0.0 6 3 51 6 0.0 6 6 52 2 0.0 6 1 0 1 53 4 0.0 6 4 54 5 0.0 6 4 1 55 6 0.0 6 6 56 6 0.0 6 6 57 7 0.0 6 7 58 4 0.0 6 4 59 5 0.0 6 5 60 2 0.0 6 2 61 4 0.0 6 4 62 6 0.0 6 6 63 6 0.0 6 6 64 10 0.0 6 10 65 7 0.0 6 5 1 1 66 7 0.0 6 7 67 3 0.0 6 3 68 6 0.0 6 6 69 2 0.0 6 2 70 10 0.0 6 10 71 7 0.0 6 6 1 72 13 0.0 6 12 1 73 6 0.0 6 6 74 9 0.0 6 9 75 7 0.0 6 7 76 12 0.0 6 12 77 10 0.0 6 10 78 2 0.0 6 2 79 11 0.0 6 11 80 13 0.0 6 12 1 81 13 0.0 6 13 82 14 0.0 6 14 83 12 0.0 6 12 84 12 0.0 6 12 85 12 0.0 6 11 0 1 86 10 0.0 6 10 87 8 0.0 6 8 88 10 0.0 6 10 89 10 0.0 6 10 90 5 0.0 6 5 91 7 0.0 6 7 92 7 0.0 6 7 93 7 0.0 6 6 1 94 8 0.0 6 8 95 6 0.0 6 6 96 8 0.0 6 8 97 5 0.0 6 5 98 9 0.0 6 8 1 99 10 0.0 6 10 100 6 0.0 6 6 101 13 0.0 6 12 1 102 15 0.0 6 14 1 103 20 0.0 6 20 104 10 0.0 6 10 105 8 0.0 6 7 1 106 18 0.0 6 18 107 11 0.0 6 11 108 12 0.0 6 11 0 0 0 0 0 1 109 19 0.0 6 18 1 110 26 0.0 6 26 111 19 0.0 6 19 112 29 0.0 6 29 113 21 0.0 6 21 114 20 0.0 6 20 115 26 0.0 6 26 116 20 0.0 6 20 117 22 0.0 6 21 1 118 24 0.0 6 24 119 27 0.0 6 26 1 120 17 0.0 6 17 121 24 0.0 6 24 122 28 0.0 6 26 1 0 1 123 20 0.0 6 20 124 38 0.0 6 38 125 41 0.0 6 41 126 27 0.0 6 27 127 29 0.0 6 29 128 32 0.0 6 31 1 129 24 0.0 6 24 130 33 0.0 6 32 1 131 36 0.0 6 36 132 28 0.0 6 28 133 47 0.0 6 47 134 35 0.0 6 35 135 37 0.0 6 36 1 136 38 0.0 6 38 137 55 0.0 6 54 0 1 138 51 0.0 6 51 139 61 0.0 6 61 140 64 0.0 6 64 141 108 0.0 6 108 142 304 0.0 6 303 1 143 665 0.0 6 664 1 144 772 0.0 6 772 145 628 0.0 6 628 146 587 0.0 6 587 147 800 0.0 6 798 2 148 674 0.0 6 672 1 0 0 1 149 668 0.0 6 667 1 150 649 0.0 6 649 151 694 0.0 6 693 1 152 933 0.0 6 933 153 627 0.0 6 626 1 154 448 0.0 6 448 155 458 0.0 6 458 156 436 0.0 6 435 1 157 400 0.0 6 397 3 158 374 0.0 6 372 2 159 448 0.0 6 445 2 1 160 437 0.0 6 437 161 422 0.0 6 422 162 458 0.0 6 456 1 1 163 475 0.0 6 475 164 426 0.0 6 425 1 165 425 0.0 6 425 166 318 0.0 6 318 167 358 0.0 6 358 168 337 0.0 6 337 169 312 0.0 6 311 1 170 300 0.0 6 298 2 171 394 0.0 6 394 172 341 0.0 6 340 1 173 326 0.0 6 324 2 174 372 0.0 6 372 175 367 0.0 6 365 2 176 279 0.0 6 277 2 177 348 0.0 6 347 1 178 317 0.0 6 316 1 179 344 0.0 6 342 1 1 180 1018 0.0 6 1018 181 393 0.0 6 392 1 182 355 0.0 6 354 1 183 332 0.0 6 330 2 184 372 0.0 6 371 1 185 392 0.0 6 387 3 2 186 363 0.0 6 360 3 187 312 0.0 6 311 0 0 1 188 340 0.0 6 338 2 189 329 0.0 6 322 7 190 278 0.0 6 277 1 191 387 0.0 6 385 2 192 376 0.0 6 372 4 193 450 0.0 6 449 1 194 490 0.0 6 485 5 195 566 0.0 6 563 3 196 601 0.0 6 594 6 1 197 573 0.0 6 569 4 198 561 0.0 6 561 199 418 0.0 6 416 2 200 453 0.0 6 448 5 201 434 0.0 6 429 5 202 469 0.0 6 464 4 0 1 203 470 0.0 6 467 3 204 674 0.0 6 669 4 1 205 671 0.0 6 666 5 206 503 0.0 6 496 7 207 596 0.0 6 592 4 208 474 0.0 6 468 4 1 0 1 209 460 0.0 6 457 3 210 523 0.0 6 519 4 211 509 0.0 6 504 5 212 461 0.0 6 460 1 213 603 0.0 6 598 5 214 574 0.0 6 570 4 215 513 0.0 6 511 2 216 542 0.0 6 539 3 217 606 0.0 6 599 7 218 491 0.0 6 486 4 1 219 437 0.0 6 435 2 220 433 0.0 6 430 3 221 419 0.0 6 416 3 222 497 0.0 6 496 0 1 223 405 0.0 6 400 5 224 447 0.0 6 446 1 225 543 0.0 6 538 4 0 1 226 684 0.0 6 676 8 227 551 0.0 6 547 4 228 550 0.0 6 546 4 229 459 0.0 6 456 3 230 516 0.0 6 510 6 231 424 0.0 6 421 3 232 492 0.0 6 488 3 1 233 450 0.0 6 444 6 234 408 0.0 6 405 3 235 381 0.0 6 377 4 236 337 0.0 6 332 5 237 385 0.0 6 383 1 1 238 353 0.0 6 351 2 239 377 0.0 6 372 4 1 240 453 0.0 6 450 2 1 241 497 0.0 6 486 11 242 508 0.0 6 505 3 243 439 0.0 6 433 6 244 320 0.0 6 310 10 245 399 0.0 6 398 1 246 234 0.0 6 231 3 247 318 0.0 6 312 6 248 199 0.0 6 197 2 249 447 0.0 6 438 9 250 279 0.0 6 277 2 251 345 0.0 6 339 6 252 289 0.0 6 283 6 253 241 0.0 6 232 8 1 254 431 0.0 6 426 5 255 329 0.0 6 320 9 256 254 0.0 6 248 6 257 378 0.0 6 369 9 258 467 0.0 6 462 5 259 469 0.0 6 461 8 260 392 0.0 6 388 3 1 261 227 0.0 6 224 2 1 262 347 0.0 6 339 8 263 552 0.0 6 539 12 1 264 304 0.0 6 295 9 265 418 0.0 6 411 7 266 262 0.0 6 258 4 267 430 0.0 6 416 14 268 369 0.0 6 360 9 269 269 0.0 6 266 3 270 136 0.0 6 134 2 271 342 0.0 6 332 10 272 902 0.0 6 882 19 1 273 512 0.0 6 499 13 274 599 0.0 6 584 13 2 275 492 0.0 6 482 9 1 276 806 0.0 6 788 17 1 277 2965 0.0 6 2915 49 1 278 1321 0.0 6 1270 46 4 1 279 61 0.0 6 58 3 280 18 0.0 6 15 1 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1150 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 4 0.0 6 3 1 64 134 0.0 6 128 6 72 3 0.0 6 3 73 5 0.0 6 5 74 17 0.0 6 17 75 11 0.0 6 11 76 17 0.0 6 17 77 11 0.0 6 11 78 25 0.0 6 25 79 21 0.0 6 21 80 19 0.0 6 19 81 12 0.0 6 12 82 15 0.0 6 15 83 23 0.0 6 23 84 8 0.0 6 8 85 5 0.0 6 5 86 11 0.0 6 11 87 10 0.0 6 10 88 8 0.0 6 8 89 12 0.0 6 12 90 13 0.0 6 13 91 9 0.0 6 9 92 7 0.0 6 7 93 8 0.0 6 8 94 9 0.0 6 9 95 7 0.0 6 7 96 11 0.0 6 11 97 2 0.0 6 2 98 8 0.0 6 8 99 13 0.0 6 13 100 5 0.0 6 5 101 6 0.0 6 6 102 8 0.0 6 8 103 6 0.0 6 6 104 11 0.0 6 11 105 11 0.0 6 11 106 5 0.0 6 5 107 4 0.0 6 4 108 4 0.0 6 4 109 3 0.0 6 3 110 22 0.0 6 22 111 30 0.0 6 30 112 10 0.0 6 10 113 7 0.0 6 7 114 10 0.0 6 10 115 1 0.0 6 1 116 7 0.0 6 7 117 2 0.0 6 2 118 8 0.0 6 8 119 5 0.0 6 5 120 4 0.0 6 4 121 4 0.0 6 4 122 2 0.0 6 2 123 4 0.0 6 4 124 3 0.0 6 3 125 7 0.0 6 7 126 3 0.0 6 3 127 4 0.0 6 4 128 10 0.0 6 10 129 9 0.0 6 9 130 3 0.0 6 3 131 3 0.0 6 3 132 2 0.0 6 2 133 8 0.0 6 8 134 10 0.0 6 10 135 10 0.0 6 10 136 5 0.0 6 5 137 6 0.0 6 6 138 13 0.0 6 13 139 4 0.0 6 4 140 5 0.0 6 5 141 4 0.0 6 4 142 6 0.0 6 6 143 5 0.0 6 5 144 3 0.0 6 3 145 5 0.0 6 5 146 3 0.0 6 3 147 5 0.0 6 5 148 6 0.0 6 6 149 7 0.0 6 7 150 9 0.0 6 8 1 151 6 0.0 6 6 152 3 0.0 6 3 153 10 0.0 6 10 154 2 0.0 6 2 155 8 0.0 6 8 157 4 0.0 6 4 158 7 0.0 6 7 159 3 0.0 6 3 160 4 0.0 6 4 161 4 0.0 6 4 162 6 0.0 6 6 163 8 0.0 6 8 164 5 0.0 6 4 1 165 6 0.0 6 6 166 3 0.0 6 3 167 4 0.0 6 4 168 4 0.0 6 4 169 2 0.0 6 2 170 8 0.0 6 8 171 8 0.0 6 8 172 7 0.0 6 7 173 2 0.0 6 2 174 4 0.0 6 4 175 3 0.0 6 3 176 3 0.0 6 3 177 4 0.0 6 4 178 5 0.0 6 5 179 6 0.0 6 6 180 3 0.0 6 3 181 4 0.0 6 4 182 4 0.0 6 4 183 6 0.0 6 6 185 1 0.0 6 1 186 8 0.0 6 8 187 5 0.0 6 5 188 5 0.0 6 5 189 4 0.0 6 4 190 5 0.0 6 5 191 3 0.0 6 3 192 2 0.0 6 2 193 10 0.0 6 10 194 7 0.0 6 7 195 4 0.0 6 4 196 4 0.0 6 4 197 2 0.0 6 2 198 3 0.0 6 3 199 3 0.0 6 3 200 2 0.0 6 2 201 5 0.0 6 4 1 202 4 0.0 6 4 203 5 0.0 6 5 204 12 0.0 6 12 205 4 0.0 6 4 206 3 0.0 6 3 207 5 0.0 6 5 208 3 0.0 6 3 209 1 0.0 6 1 210 5 0.0 6 5 211 2 0.0 6 2 212 4 0.0 6 4 213 7 0.0 6 7 214 3 0.0 6 3 215 1 0.0 6 1 216 3 0.0 6 3 217 2 0.0 6 2 218 6 0.0 6 6 219 2 0.0 6 2 221 2 0.0 6 2 222 2 0.0 6 1 1 224 2 0.0 6 2 225 2 0.0 6 2 226 1 0.0 6 1 227 1 0.0 6 1 228 6 0.0 6 6 229 4 0.0 6 4 231 1 0.0 6 1 232 3 0.0 6 3 233 1 0.0 6 1 235 1 0.0 6 1 237 3 0.0 6 3 240 3 0.0 6 3 241 1 0.0 6 1 242 1 0.0 6 1 247 3 0.0 6 3 248 1 0.0 6 1 249 2 0.0 6 2 250 1 0.0 6 1 253 2 0.0 6 2 257 2 0.0 6 2 261 1 0.0 6 1 262 2 0.0 6 2 268 1 0.0 6 1 270 1 0.0 6 1 273 1 0.0 6 1 281 1 0.0 6 1 286 1 0.0 6 1 289 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 67414 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 8 1 1.0 2 0 0 1 10 4 0.1 3 4 11 2 0.0 3 2 12 10 0.0 3 10 13 10 0.0 3 9 1 14 15 0.0 4 14 0 1 15 1 0.0 4 1 16 8 0.0 4 8 17 43 0.0 5 41 2 18 24 0.0 5 22 2 19 13 0.0 5 13 20 60 0.0 6 57 3 21 46 0.0 6 45 1 22 28 0.0 6 27 1 23 21 0.0 6 20 1 24 14 0.0 6 14 25 14 0.0 6 12 2 26 19 0.0 6 18 1 27 17 0.0 6 17 28 5 0.0 6 5 29 13 0.0 6 11 2 30 15 0.0 6 14 1 31 11 0.0 6 10 1 32 11 0.0 6 8 2 0 0 1 33 9 0.0 6 8 1 34 11 0.0 6 10 1 35 9 0.0 6 7 2 36 11 0.0 6 10 1 37 7 0.0 6 5 1 1 38 13 0.0 6 13 39 6 0.0 6 6 40 14 0.0 6 14 41 14 0.0 6 12 2 42 10 0.0 6 10 43 15 0.0 6 13 2 44 29 0.0 6 25 4 45 7 0.0 6 7 46 16 0.0 6 13 3 47 22 0.0 6 21 1 48 22 0.0 6 20 1 0 1 49 31 0.0 6 28 3 50 23 0.0 6 19 4 51 13 0.0 6 11 2 52 17 0.0 6 16 1 53 22 0.0 6 21 1 54 15 0.0 6 15 55 20 0.0 6 18 1 1 56 23 0.0 6 23 57 23 0.0 6 20 2 1 58 19 0.0 6 14 5 59 22 0.0 6 21 0 1 60 20 0.0 6 18 2 61 29 0.0 6 27 1 0 1 62 11 0.0 6 8 2 1 63 17 0.0 6 15 1 1 64 26 0.0 6 26 65 33 0.0 6 28 2 1 0 1 0 1 66 19 0.0 6 18 1 67 38 0.0 6 33 3 2 68 109 0.0 6 107 1 0 0 0 1 69 47 0.0 6 40 6 1 70 80 0.0 6 74 5 0 0 0 0 1 71 38 0.0 6 35 3 72 48 0.0 6 47 0 1 73 22 0.0 6 21 1 74 26 0.0 6 24 1 0 0 0 1 75 35 0.0 6 31 2 0 0 0 0 2 76 42 0.0 6 36 5 0 1 77 24 0.0 6 22 1 0 0 0 0 1 78 42 0.0 6 37 4 1 79 41 0.0 6 41 80 50 0.0 6 46 4 81 46 0.0 6 45 1 82 51 0.0 6 49 1 0 0 0 0 1 83 72 0.0 6 72 84 88 0.0 6 88 85 44 0.0 6 41 3 86 38 0.0 6 37 1 87 54 0.0 6 48 5 1 88 40 0.0 6 38 2 89 35 0.0 6 34 1 90 50 0.0 6 48 1 1 91 51 0.0 6 50 0 1 92 67 0.0 6 63 4 93 69 0.0 6 65 3 0 1 94 94 0.0 6 90 3 1 95 75 0.0 6 67 7 1 96 58 0.0 6 57 1 97 81 0.0 6 77 2 0 1 1 98 67 0.0 6 65 1 1 99 83 0.0 6 79 3 0 0 0 1 100 69 0.0 6 65 4 101 90 0.0 6 88 1 0 1 102 79 0.0 6 75 3 0 0 1 103 88 0.0 6 86 2 104 129 0.0 6 124 3 1 0 0 1 105 187 0.0 6 186 1 106 93 0.0 6 88 5 107 114 0.0 6 110 4 108 87 0.0 6 86 1 109 99 0.0 6 95 4 110 95 0.0 6 92 2 1 111 98 0.0 6 96 2 112 124 0.0 6 118 6 113 142 0.0 6 139 3 114 125 0.0 6 123 2 115 117 0.0 6 113 3 1 116 126 0.0 6 124 2 117 129 0.0 6 125 4 118 156 0.0 6 156 119 143 0.0 6 140 3 120 180 0.0 6 173 5 0 0 1 1 121 181 0.0 6 177 4 122 187 0.0 6 183 3 0 0 1 123 193 0.0 6 192 1 124 186 0.0 6 180 5 1 125 228 0.0 6 224 4 126 246 0.0 6 238 8 127 285 0.0 6 280 5 128 296 0.0 6 291 5 129 265 0.0 6 261 3 0 1 130 296 0.0 6 292 4 131 347 0.0 6 343 3 0 0 0 1 132 483 0.0 6 472 10 1 133 419 0.0 6 410 7 1 0 1 134 483 0.0 6 480 3 135 505 0.0 6 496 8 1 136 409 0.0 6 403 5 1 137 504 0.0 6 496 7 0 0 1 138 419 0.0 6 413 6 139 425 0.0 6 420 3 2 140 586 0.0 6 581 5 141 748 0.0 6 746 2 142 1031 0.0 6 1022 7 1 0 0 0 1 143 1147 0.0 6 1132 10 2 1 1 1 144 1081 0.0 6 1073 8 145 1064 0.0 6 1054 7 2 0 1 146 1096 0.0 6 1080 14 1 0 0 0 1 147 1050 0.0 6 1039 10 0 0 0 1 148 1135 0.0 6 1122 11 2 149 1313 0.0 6 1294 16 2 0 0 1 150 1367 0.0 6 1349 16 0 0 1 1 151 1867 0.0 6 1854 12 1 152 1331 0.0 6 1313 17 0 1 153 1252 0.0 6 1233 18 1 154 1363 0.0 6 1347 15 0 0 0 0 1 155 1022 0.0 6 1011 7 1 2 1 156 1037 0.0 6 1026 11 157 977 0.0 6 963 11 0 0 0 0 3 158 1080 0.0 6 1070 9 1 159 985 0.0 6 974 11 160 1012 0.0 6 996 15 1 161 967 0.0 6 959 7 0 0 1 162 891 0.0 6 883 8 163 767 0.0 6 752 14 1 164 804 0.0 6 798 4 1 0 0 0 1 165 754 0.0 6 745 9 166 822 0.0 6 812 10 167 816 0.0 6 805 9 0 0 0 1 1 168 873 0.0 6 853 18 0 1 1 169 747 0.0 6 738 7 0 0 0 0 2 170 618 0.0 6 608 7 1 1 1 171 800 0.0 6 788 11 0 0 1 172 694 0.0 6 685 7 1 1 173 703 0.0 6 696 6 1 174 750 0.0 6 739 10 0 1 175 879 0.0 6 865 11 2 1 176 645 0.0 6 637 7 0 1 177 777 0.0 6 768 9 178 615 0.0 6 610 5 179 770 0.0 6 760 10 180 595 0.0 6 583 11 1 181 768 0.0 6 746 18 2 0 2 182 334 0.0 6 329 5 183 511 0.0 6 501 9 1 184 590 0.0 6 579 10 1 185 660 0.0 6 653 7 186 910 0.0 6 883 25 0 0 1 0 1 187 685 0.0 6 668 17 188 636 0.0 6 620 16 189 612 0.0 6 599 12 0 1 190 891 0.0 6 871 20 191 808 0.0 6 781 26 0 0 1 192 653 0.0 6 639 14 193 395 0.0 6 388 6 0 1 194 594 0.0 6 582 11 0 0 1 195 627 0.0 6 610 15 0 0 2 196 1136 0.0 6 1098 36 0 0 2 197 503 0.0 6 490 12 1 198 676 0.0 6 655 21 199 993 0.0 6 967 24 2 200 350 0.0 6 349 1 201 408 0.0 6 398 8 0 0 2 202 211 0.0 6 204 6 1 203 180 0.0 6 174 6 204 239 0.0 6 230 9 205 118 0.0 6 113 5 206 119 0.0 6 116 3 207 77 0.0 6 77 208 125 0.0 6 122 3 209 114 0.0 6 111 3 210 97 0.0 6 94 2 1 211 115 0.0 6 113 2 212 117 0.0 6 117 213 52 0.0 6 52 214 81 0.0 6 78 2 0 0 0 1 215 118 0.0 6 112 5 1 216 128 0.0 6 124 4 217 90 0.0 6 90 218 82 0.0 6 82 219 82 0.0 6 79 3 220 42 0.0 6 38 4 221 27 0.0 6 27 222 91 0.0 6 85 6 223 193 0.0 6 188 4 1 224 35 0.0 6 35 225 83 0.0 6 81 2 226 52 0.0 6 50 2 227 45 0.0 6 44 1 228 55 0.0 6 54 1 229 49 0.0 6 49 230 37 0.0 6 35 2 231 20 0.0 6 18 0 2 232 70 0.0 6 68 2 233 66 0.0 6 65 1 234 7 0.0 6 7 235 8 0.0 6 7 1 236 2 0.0 6 2 237 2 0.0 6 2 239 6 0.0 6 4 2 240 22 0.0 6 22 241 5 0.0 6 5 242 32 0.0 6 32 243 25 0.0 6 22 3 244 10 0.0 6 9 1 245 6 0.0 6 6 246 2 0.0 6 2 247 2 0.0 6 2 248 3 0.0 6 3 249 2 0.0 6 2 250 2 0.0 6 2 251 2 0.0 6 2 252 1 0.0 6 1 253 2 0.0 6 1 1 262 1 0.0 6 1 277 1 0.0 6 1 278 1 0.0 6 1 279 8 0.0 6 8 280 46 0.0 6 44 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1097 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 6 60 16.7 1 0 0 60 55 1 0.0 6 1 58 1 0.0 6 1 59 5 0.0 6 5 68 1 0.0 6 1 70 1 0.0 6 1 71 14 0.0 6 14 72 18 0.0 6 18 73 27 0.0 6 27 74 9 0.0 6 9 75 16 0.0 6 16 76 24 0.0 6 24 77 23 0.0 6 23 78 19 0.0 6 19 79 19 0.0 6 19 80 24 0.0 6 23 1 81 27 0.0 6 26 0 0 0 1 82 20 0.0 6 19 1 83 10 0.0 6 10 84 14 0.0 6 13 0 0 0 1 85 19 0.0 6 19 86 13 0.0 6 13 87 11 0.0 6 11 88 13 0.0 6 13 89 10 0.0 6 10 90 12 0.0 6 12 91 16 0.0 6 16 92 16 0.0 6 16 93 12 0.0 6 12 94 11 0.0 6 11 95 10 0.0 6 10 96 12 0.0 6 12 97 9 0.0 6 9 98 7 0.0 6 7 99 11 0.0 6 11 100 10 0.0 6 10 101 10 0.0 6 10 102 7 0.0 6 7 103 8 0.0 6 8 104 11 0.0 6 11 105 6 0.0 6 6 106 2 0.0 6 2 107 7 0.0 6 7 108 22 0.0 6 22 109 28 0.0 6 28 110 7 0.0 6 7 111 7 0.0 6 7 112 9 0.0 6 9 113 9 0.0 6 9 114 4 0.0 6 4 115 5 0.0 6 5 116 9 0.0 6 9 117 9 0.0 6 9 118 6 0.0 6 6 119 3 0.0 6 3 120 4 0.0 6 4 121 9 0.0 6 9 122 5 0.0 6 5 123 7 0.0 6 7 124 4 0.0 6 4 125 6 0.0 6 6 126 7 0.0 6 7 127 2 0.0 6 2 128 2 0.0 6 2 129 5 0.0 6 5 130 2 0.0 6 2 131 3 0.0 6 3 132 4 0.0 6 4 133 14 0.0 6 14 134 5 0.0 6 5 135 10 0.0 6 10 136 5 0.0 6 5 137 4 0.0 6 4 138 5 0.0 6 5 139 6 0.0 6 6 140 8 0.0 6 8 141 7 0.0 6 7 142 2 0.0 6 2 143 9 0.0 6 9 144 5 0.0 6 5 145 5 0.0 6 5 146 9 0.0 6 9 147 8 0.0 6 8 148 5 0.0 6 5 149 7 0.0 6 6 1 150 5 0.0 6 5 151 6 0.0 6 6 152 7 0.0 6 7 153 2 0.0 6 2 154 6 0.0 6 6 155 2 0.0 6 2 156 7 0.0 6 7 157 4 0.0 6 4 158 9 0.0 6 9 159 4 0.0 6 4 160 5 0.0 6 5 161 6 0.0 6 6 162 2 0.0 6 2 163 9 0.0 6 9 164 7 0.0 6 7 165 4 0.0 6 4 166 10 0.0 6 10 167 7 0.0 6 7 168 8 0.0 6 8 169 9 0.0 6 9 170 5 0.0 6 5 171 7 0.0 6 7 172 3 0.0 6 3 173 2 0.0 6 2 174 4 0.0 6 4 175 3 0.0 6 3 176 2 0.0 6 2 177 3 0.0 6 3 178 5 0.0 6 5 179 3 0.0 6 3 181 1 0.0 6 1 182 3 0.0 6 3 183 4 0.0 6 4 184 10 0.0 6 10 185 1 0.0 6 1 186 3 0.0 6 3 187 5 0.0 6 5 188 2 0.0 6 2 189 4 0.0 6 4 190 2 0.0 6 2 191 3 0.0 6 3 193 4 0.0 6 4 194 2 0.0 6 2 195 1 0.0 6 1 196 2 0.0 6 2 197 1 0.0 6 1 198 2 0.0 6 2 199 4 0.0 6 4 200 1 0.0 6 1 201 1 0.0 6 1 202 3 0.0 6 3 204 1 0.0 6 1 205 2 0.0 6 2 206 2 0.0 6 2 207 1 0.0 6 1 208 1 0.0 6 1 212 1 0.0 6 1 216 1 0.0 6 1 219 2 0.0 6 2 223 2 0.0 6 2 230 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_09_146_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_09_373_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_09_146_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_09_373_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.65 s (68 µs/read; 0.88 M reads/minute). === Summary === Total read pairs processed: 67,884 Read 1 with adapter: 67,879 (100.0%) Read 2 with adapter: 67,857 (100.0%) == Read fate breakdown == Pairs that were too short: 15 (0.0%) Pairs discarded as untrimmed: 32 (0.0%) Pairs written (passing filters): 67,837 (99.9%) Total basepairs processed: 28,652,243 bp Read 1: 16,021,213 bp Read 2: 12,631,030 bp Total written (filtered): 4,365,819 bp (15.2%) Read 1: 2,339,129 bp Read 2: 2,026,690 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 65860 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1060.7 0 1 4 2 265.2 1 2 6 2 16.6 1 1 0 1 7 10 4.1 2 4 0 6 9 3 0.3 2 3 12 1 0.0 3 0 0 0 1 13 2 0.0 3 2 14 1 0.0 4 1 16 4 0.0 4 4 17 4 0.0 5 3 1 18 2 0.0 5 2 19 3 0.0 5 3 20 57 0.0 6 55 2 21 43 0.0 6 42 1 22 25 0.0 6 23 2 23 21 0.0 6 21 24 17 0.0 6 16 1 25 14 0.0 6 14 26 11 0.0 6 11 27 7 0.0 6 7 28 7 0.0 6 7 29 10 0.0 6 9 1 30 3 0.0 6 3 31 6 0.0 6 5 0 0 0 0 1 32 6 0.0 6 6 33 5 0.0 6 5 34 8 0.0 6 7 1 35 5 0.0 6 5 36 4 0.0 6 4 37 9 0.0 6 9 38 1 0.0 6 1 39 8 0.0 6 8 40 9 0.0 6 8 1 41 10 0.0 6 9 1 42 3 0.0 6 2 1 43 4 0.0 6 4 44 6 0.0 6 6 45 5 0.0 6 5 46 7 0.0 6 6 1 47 6 0.0 6 6 48 6 0.0 6 6 49 6 0.0 6 6 50 16 0.0 6 14 1 0 0 0 0 1 51 2 0.0 6 2 52 4 0.0 6 3 1 53 4 0.0 6 4 54 3 0.0 6 3 55 3 0.0 6 3 56 6 0.0 6 6 57 5 0.0 6 5 58 8 0.0 6 8 59 4 0.0 6 4 60 2 0.0 6 2 61 2 0.0 6 2 62 5 0.0 6 5 63 10 0.0 6 10 64 7 0.0 6 6 1 65 7 0.0 6 6 1 66 4 0.0 6 4 67 11 0.0 6 10 1 68 11 0.0 6 11 69 11 0.0 6 11 70 8 0.0 6 8 71 13 0.0 6 12 1 72 9 0.0 6 9 73 7 0.0 6 6 1 74 5 0.0 6 5 75 10 0.0 6 10 76 8 0.0 6 8 77 4 0.0 6 4 78 7 0.0 6 7 79 10 0.0 6 10 80 6 0.0 6 6 81 10 0.0 6 10 82 14 0.0 6 13 1 83 18 0.0 6 18 84 4 0.0 6 4 85 11 0.0 6 11 86 13 0.0 6 12 1 87 11 0.0 6 10 1 88 4 0.0 6 4 89 11 0.0 6 11 90 10 0.0 6 9 0 0 0 0 1 91 11 0.0 6 10 1 92 5 0.0 6 5 93 9 0.0 6 9 94 7 0.0 6 7 95 10 0.0 6 9 1 96 7 0.0 6 6 1 97 25 0.0 6 23 1 0 1 98 13 0.0 6 13 99 21 0.0 6 21 100 8 0.0 6 6 1 0 1 101 18 0.0 6 18 102 11 0.0 6 10 1 103 17 0.0 6 16 0 0 1 104 18 0.0 6 16 1 0 1 105 10 0.0 6 10 106 10 0.0 6 10 107 17 0.0 6 17 108 14 0.0 6 13 1 109 24 0.0 6 23 1 110 16 0.0 6 16 111 18 0.0 6 17 0 1 112 29 0.0 6 29 113 15 0.0 6 14 1 114 30 0.0 6 30 115 17 0.0 6 16 1 116 22 0.0 6 22 117 20 0.0 6 20 118 22 0.0 6 21 1 119 13 0.0 6 13 120 22 0.0 6 20 2 121 19 0.0 6 19 122 26 0.0 6 26 123 26 0.0 6 25 1 124 27 0.0 6 25 1 0 0 0 1 125 18 0.0 6 18 126 30 0.0 6 29 1 127 48 0.0 6 47 0 0 0 0 0 1 128 49 0.0 6 49 129 32 0.0 6 31 1 130 39 0.0 6 39 131 35 0.0 6 34 1 132 32 0.0 6 30 2 133 38 0.0 6 36 2 134 109 0.0 6 108 1 135 56 0.0 6 54 1 0 0 0 1 136 43 0.0 6 43 137 36 0.0 6 35 0 0 0 0 1 138 55 0.0 6 54 1 139 72 0.0 6 72 140 169 0.0 6 169 141 320 0.0 6 317 3 142 409 0.0 6 407 2 143 369 0.0 6 369 144 301 0.0 6 299 2 145 514 0.0 6 514 146 450 0.0 6 449 1 147 348 0.0 6 346 2 148 501 0.0 6 499 1 0 1 149 402 0.0 6 400 2 150 533 0.0 6 532 1 151 317 0.0 6 317 152 244 0.0 6 244 153 288 0.0 6 287 1 154 295 0.0 6 295 155 257 0.0 6 255 2 156 364 0.0 6 364 157 368 0.0 6 367 1 158 365 0.0 6 364 1 159 379 0.0 6 378 1 160 378 0.0 6 375 3 161 406 0.0 6 402 2 0 0 1 0 1 162 349 0.0 6 348 1 163 364 0.0 6 362 1 0 1 164 244 0.0 6 241 1 0 0 0 1 1 165 272 0.0 6 272 166 382 0.0 6 382 167 369 0.0 6 368 0 1 168 346 0.0 6 344 2 169 383 0.0 6 382 1 170 381 0.0 6 381 171 341 0.0 6 335 4 0 1 1 172 456 0.0 6 456 173 378 0.0 6 377 1 174 266 0.0 6 265 1 175 359 0.0 6 355 4 176 325 0.0 6 324 1 177 329 0.0 6 327 2 178 838 0.0 6 835 3 179 437 0.0 6 435 2 180 363 0.0 6 360 2 1 181 404 0.0 6 404 182 347 0.0 6 347 183 353 0.0 6 351 1 1 184 409 0.0 6 408 1 185 421 0.0 6 418 3 186 365 0.0 6 362 3 187 397 0.0 6 395 2 188 409 0.0 6 405 3 0 0 1 189 433 0.0 6 428 4 0 1 190 699 0.0 6 697 2 191 523 0.0 6 518 4 1 192 610 0.0 6 608 2 193 716 0.0 6 713 2 0 1 194 639 0.0 6 638 1 195 681 0.0 6 678 3 196 841 0.0 6 836 4 0 0 1 197 904 0.0 6 898 5 0 0 0 1 198 766 0.0 6 759 4 0 2 1 199 638 0.0 6 635 3 200 674 0.0 6 673 1 201 680 0.0 6 670 9 1 202 1008 0.0 6 1004 2 1 0 0 0 1 203 678 0.0 6 677 1 204 774 0.0 6 767 5 1 0 1 205 959 0.0 6 955 3 0 0 0 0 1 206 761 0.0 6 757 4 207 861 0.0 6 856 5 208 549 0.0 6 546 3 209 632 0.0 6 629 2 0 1 210 708 0.0 6 705 3 211 820 0.0 6 813 7 212 751 0.0 6 748 3 213 909 0.0 6 900 7 0 0 1 1 214 1091 0.0 6 1081 9 0 1 215 829 0.0 6 823 4 0 1 0 0 1 216 909 0.0 6 906 2 0 0 1 217 915 0.0 6 909 6 218 807 0.0 6 799 8 219 721 0.0 6 719 1 0 1 220 743 0.0 6 738 5 221 677 0.0 6 674 3 222 777 0.0 6 770 7 223 810 0.0 6 809 1 224 909 0.0 6 906 3 225 767 0.0 6 766 1 226 674 0.0 6 670 3 0 0 0 1 227 572 0.0 6 569 3 228 584 0.0 6 581 3 229 561 0.0 6 558 2 1 230 543 0.0 6 540 3 231 591 0.0 6 589 2 232 584 0.0 6 579 4 0 1 233 576 0.0 6 572 3 0 0 1 234 385 0.0 6 382 2 1 235 445 0.0 6 441 4 236 428 0.0 6 427 1 237 417 0.0 6 413 3 1 238 448 0.0 6 442 5 0 0 1 239 424 0.0 6 418 6 240 445 0.0 6 441 4 241 379 0.0 6 375 4 242 348 0.0 6 346 2 243 338 0.0 6 336 1 0 0 0 1 244 283 0.0 6 280 2 0 0 0 0 1 245 262 0.0 6 261 1 246 249 0.0 6 249 247 221 0.0 6 220 1 248 250 0.0 6 247 3 249 239 0.0 6 239 250 206 0.0 6 203 2 1 251 182 0.0 6 178 4 252 217 0.0 6 215 2 253 172 0.0 6 171 1 254 178 0.0 6 176 2 255 191 0.0 6 191 256 198 0.0 6 195 2 0 0 0 0 1 257 210 0.0 6 209 1 258 184 0.0 6 182 2 259 214 0.0 6 212 2 260 150 0.0 6 148 2 261 135 0.0 6 133 2 262 141 0.0 6 141 263 135 0.0 6 135 264 110 0.0 6 110 265 141 0.0 6 139 2 266 127 0.0 6 127 267 106 0.0 6 106 268 102 0.0 6 102 269 121 0.0 6 121 270 99 0.0 6 98 1 271 114 0.0 6 114 272 116 0.0 6 116 273 130 0.0 6 129 1 274 132 0.0 6 131 1 275 155 0.0 6 152 3 276 372 0.0 6 366 6 277 904 0.0 6 889 12 2 1 278 1916 0.0 6 1884 30 0 1 1 279 66 0.0 6 65 1 280 5 0.0 6 5 281 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2019 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 3 0.0 6 3 63 9 0.0 6 8 1 64 334 0.0 6 327 6 1 65 1 0.0 6 1 72 1 0.0 6 1 73 8 0.0 6 8 74 13 0.0 6 13 75 12 0.0 6 12 76 12 0.0 6 12 77 7 0.0 6 7 78 11 0.0 6 11 79 19 0.0 6 19 80 13 0.0 6 12 1 81 11 0.0 6 11 82 10 0.0 6 10 83 16 0.0 6 16 84 8 0.0 6 8 85 7 0.0 6 7 86 7 0.0 6 7 87 7 0.0 6 7 88 14 0.0 6 14 89 11 0.0 6 11 90 10 0.0 6 9 1 91 12 0.0 6 12 92 12 0.0 6 12 93 7 0.0 6 7 94 11 0.0 6 9 1 1 95 10 0.0 6 10 96 10 0.0 6 10 97 5 0.0 6 5 98 8 0.0 6 8 99 13 0.0 6 13 100 7 0.0 6 7 101 9 0.0 6 9 102 12 0.0 6 12 103 6 0.0 6 6 104 13 0.0 6 13 105 10 0.0 6 10 106 14 0.0 6 14 107 7 0.0 6 7 108 10 0.0 6 10 109 8 0.0 6 8 110 10 0.0 6 10 111 30 0.0 6 30 112 8 0.0 6 8 113 14 0.0 6 14 114 6 0.0 6 6 115 7 0.0 6 7 116 6 0.0 6 6 117 8 0.0 6 7 1 118 10 0.0 6 10 119 3 0.0 6 3 120 5 0.0 6 5 121 10 0.0 6 10 122 3 0.0 6 3 123 10 0.0 6 10 124 7 0.0 6 7 125 8 0.0 6 8 126 14 0.0 6 14 127 13 0.0 6 13 128 9 0.0 6 9 129 10 0.0 6 10 130 11 0.0 6 11 131 5 0.0 6 5 132 8 0.0 6 8 133 13 0.0 6 13 134 29 0.0 6 29 135 20 0.0 6 20 136 16 0.0 6 16 137 12 0.0 6 12 138 5 0.0 6 5 139 13 0.0 6 13 140 10 0.0 6 10 141 11 0.0 6 11 142 5 0.0 6 5 143 7 0.0 6 7 144 6 0.0 6 6 145 7 0.0 6 7 146 12 0.0 6 12 147 5 0.0 6 5 148 7 0.0 6 7 149 16 0.0 6 16 150 13 0.0 6 13 151 2 0.0 6 2 152 13 0.0 6 13 153 18 0.0 6 18 154 17 0.0 6 17 155 10 0.0 6 10 156 18 0.0 6 18 157 17 0.0 6 17 158 10 0.0 6 10 159 15 0.0 6 15 160 6 0.0 6 6 161 15 0.0 6 15 162 10 0.0 6 10 163 24 0.0 6 24 164 9 0.0 6 9 165 15 0.0 6 15 166 10 0.0 6 10 167 10 0.0 6 10 168 13 0.0 6 13 169 5 0.0 6 5 170 14 0.0 6 14 171 16 0.0 6 16 172 9 0.0 6 9 173 11 0.0 6 11 174 9 0.0 6 9 175 18 0.0 6 17 1 176 12 0.0 6 12 177 16 0.0 6 16 178 12 0.0 6 12 179 10 0.0 6 10 180 9 0.0 6 9 181 5 0.0 6 5 182 5 0.0 6 5 183 6 0.0 6 6 184 10 0.0 6 10 185 3 0.0 6 3 186 15 0.0 6 15 187 11 0.0 6 11 188 14 0.0 6 14 189 11 0.0 6 11 190 14 0.0 6 14 191 10 0.0 6 10 192 13 0.0 6 13 193 14 0.0 6 14 194 18 0.0 6 18 195 13 0.0 6 13 196 5 0.0 6 5 197 10 0.0 6 10 198 20 0.0 6 20 199 15 0.0 6 15 200 6 0.0 6 6 201 9 0.0 6 9 202 5 0.0 6 5 203 13 0.0 6 13 204 15 0.0 6 15 205 3 0.0 6 3 206 7 0.0 6 7 207 10 0.0 6 10 208 9 0.0 6 9 209 2 0.0 6 2 210 3 0.0 6 3 211 8 0.0 6 8 212 9 0.0 6 9 213 12 0.0 6 12 214 10 0.0 6 10 215 9 0.0 6 9 216 6 0.0 6 6 217 3 0.0 6 3 218 2 0.0 6 2 219 4 0.0 6 4 220 3 0.0 6 3 221 7 0.0 6 7 222 6 0.0 6 6 223 3 0.0 6 3 224 3 0.0 6 3 225 2 0.0 6 2 226 2 0.0 6 2 227 4 0.0 6 4 228 7 0.0 6 7 229 3 0.0 6 3 230 3 0.0 6 3 231 3 0.0 6 3 232 9 0.0 6 9 233 3 0.0 6 3 234 3 0.0 6 3 235 3 0.0 6 3 236 2 0.0 6 2 237 2 0.0 6 2 238 2 0.0 6 2 239 3 0.0 6 3 241 1 0.0 6 1 242 5 0.0 6 5 243 1 0.0 6 1 244 3 0.0 6 3 245 2 0.0 6 2 246 3 0.0 6 3 247 2 0.0 6 2 249 2 0.0 6 2 250 5 0.0 6 5 251 3 0.0 6 3 252 1 0.0 6 1 254 2 0.0 6 2 255 2 0.0 6 2 256 1 0.0 6 1 257 1 0.0 6 1 259 1 0.0 6 1 260 4 0.0 6 4 261 3 0.0 6 3 262 2 0.0 6 2 263 1 0.0 6 1 264 1 0.0 6 1 265 1 0.0 6 1 266 1 0.0 6 1 267 2 0.0 6 2 268 2 0.0 6 2 269 3 0.0 6 3 270 1 0.0 6 1 272 1 0.0 6 1 273 1 0.0 6 1 276 1 0.0 6 1 278 1 0.0 6 1 281 1 0.0 6 1 284 1 0.0 6 1 288 2 0.0 6 2 290 1 0.0 6 1 294 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 66200 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 4.1 2 1 11 2 0.0 3 2 12 4 0.0 3 4 13 6 0.0 3 3 3 14 14 0.0 4 13 1 16 7 0.0 4 6 1 17 20 0.0 5 19 1 18 2 0.0 5 2 19 44 0.0 5 41 2 1 20 80 0.0 6 76 4 21 40 0.0 6 40 22 31 0.0 6 29 1 1 23 15 0.0 6 13 1 1 24 19 0.0 6 19 25 20 0.0 6 19 1 26 24 0.0 6 22 2 27 16 0.0 6 15 1 28 23 0.0 6 21 2 29 12 0.0 6 12 30 7 0.0 6 6 1 31 11 0.0 6 9 2 32 22 0.0 6 18 3 0 0 0 0 1 33 13 0.0 6 12 1 34 15 0.0 6 14 1 35 8 0.0 6 6 2 36 12 0.0 6 11 1 37 14 0.0 6 13 1 38 15 0.0 6 11 1 0 1 2 39 10 0.0 6 10 40 10 0.0 6 9 1 41 17 0.0 6 15 2 42 11 0.0 6 9 2 43 14 0.0 6 12 1 0 0 1 44 21 0.0 6 17 4 45 20 0.0 6 19 0 0 0 1 46 34 0.0 6 33 0 0 1 47 13 0.0 6 10 3 48 15 0.0 6 15 49 25 0.0 6 23 1 0 0 1 50 24 0.0 6 21 2 1 51 20 0.0 6 19 0 0 0 0 1 52 27 0.0 6 23 3 1 53 22 0.0 6 20 2 54 17 0.0 6 14 1 0 1 0 0 1 55 19 0.0 6 17 1 0 1 56 20 0.0 6 17 2 1 57 18 0.0 6 17 1 58 17 0.0 6 15 2 59 33 0.0 6 30 3 60 17 0.0 6 17 61 26 0.0 6 26 62 38 0.0 6 37 1 63 28 0.0 6 26 1 1 64 17 0.0 6 16 1 65 27 0.0 6 22 5 66 26 0.0 6 24 2 67 26 0.0 6 23 2 1 68 27 0.0 6 26 1 69 45 0.0 6 44 1 70 114 0.0 6 109 2 0 2 0 1 71 56 0.0 6 53 2 0 0 0 0 1 72 61 0.0 6 57 3 1 73 35 0.0 6 32 2 1 74 56 0.0 6 48 7 0 1 75 39 0.0 6 38 0 0 1 76 28 0.0 6 28 77 51 0.0 6 47 4 78 40 0.0 6 38 1 1 79 44 0.0 6 41 3 80 29 0.0 6 26 1 2 81 47 0.0 6 47 82 61 0.0 6 59 1 0 0 1 83 57 0.0 6 53 4 84 55 0.0 6 52 3 85 68 0.0 6 67 1 86 76 0.0 6 69 5 0 0 1 1 87 59 0.0 6 59 88 51 0.0 6 47 3 0 0 0 1 89 44 0.0 6 43 1 90 49 0.0 6 48 1 91 57 0.0 6 56 1 92 61 0.0 6 55 6 93 65 0.0 6 63 2 94 69 0.0 6 67 1 1 95 63 0.0 6 60 3 96 94 0.0 6 88 4 2 97 79 0.0 6 77 2 98 86 0.0 6 81 4 0 0 0 0 1 99 102 0.0 6 96 5 1 100 105 0.0 6 101 3 0 1 101 96 0.0 6 91 4 1 102 70 0.0 6 69 1 103 95 0.0 6 95 104 96 0.0 6 92 4 105 96 0.0 6 94 1 1 106 123 0.0 6 119 3 0 0 0 1 107 206 0.0 6 194 10 0 0 0 2 108 123 0.0 6 118 5 109 141 0.0 6 137 3 0 0 0 1 110 112 0.0 6 110 0 0 0 1 0 1 111 132 0.0 6 129 3 112 141 0.0 6 140 1 113 144 0.0 6 137 5 1 0 1 114 150 0.0 6 145 4 1 115 190 0.0 6 180 9 1 116 157 0.0 6 154 2 1 117 136 0.0 6 131 4 1 118 189 0.0 6 182 7 119 172 0.0 6 165 7 120 200 0.0 6 198 2 121 240 0.0 6 233 4 0 1 2 122 195 0.0 6 192 3 123 166 0.0 6 161 4 1 124 199 0.0 6 193 5 1 125 224 0.0 6 220 4 126 232 0.0 6 230 2 127 289 0.0 6 287 1 0 0 0 1 128 323 0.0 6 319 3 0 0 0 0 1 129 342 0.0 6 328 11 1 0 1 1 130 362 0.0 6 359 3 131 373 0.0 6 366 6 1 132 551 0.0 6 535 12 0 0 0 3 1 133 459 0.0 6 446 9 1 0 0 2 1 134 449 0.0 6 435 11 1 1 0 1 135 452 0.0 6 442 8 0 0 0 1 1 136 481 0.0 6 475 5 0 0 0 1 137 543 0.0 6 537 6 138 502 0.0 6 495 6 0 0 1 139 495 0.0 6 488 7 140 517 0.0 6 509 6 0 2 141 597 0.0 6 585 9 0 2 0 0 1 142 783 0.0 6 773 8 0 0 0 0 2 143 863 0.0 6 854 6 0 0 1 2 144 890 0.0 6 879 7 1 1 1 0 1 145 919 0.0 6 896 21 0 1 0 1 146 1012 0.0 6 999 12 1 147 1045 0.0 6 1032 11 1 1 148 978 0.0 6 959 16 0 1 0 1 1 149 1177 0.0 6 1157 19 1 150 1124 0.0 6 1113 9 1 0 0 0 1 151 1296 0.0 6 1277 15 0 0 1 1 2 152 1260 0.0 6 1240 17 0 0 0 2 1 153 1513 0.0 6 1493 17 0 0 2 0 1 154 1262 0.0 6 1249 11 0 0 1 1 155 1466 0.0 6 1445 18 1 0 1 1 156 1381 0.0 6 1361 17 1 1 0 0 1 157 1178 0.0 6 1163 12 0 2 1 158 1295 0.0 6 1276 13 0 1 2 1 2 159 1096 0.0 6 1083 11 0 0 1 0 1 160 1271 0.0 6 1257 12 0 0 1 1 161 1188 0.0 6 1168 14 2 1 2 0 1 162 1096 0.0 6 1081 11 1 0 0 1 2 163 1052 0.0 6 1042 10 164 1036 0.0 6 1024 10 0 0 0 2 165 863 0.0 6 857 5 0 0 0 0 1 166 1032 0.0 6 1019 12 0 0 1 167 919 0.0 6 912 6 0 0 0 1 168 835 0.0 6 825 8 1 0 1 169 1063 0.0 6 1053 9 0 0 0 0 1 170 852 0.0 6 849 2 0 0 0 1 171 1053 0.0 6 1040 11 0 1 0 0 1 172 790 0.0 6 781 8 0 1 173 879 0.0 6 867 7 2 1 1 0 1 174 964 0.0 6 951 9 0 1 0 0 3 175 896 0.0 6 892 3 0 0 1 176 901 0.0 6 887 13 1 177 853 0.0 6 849 4 178 787 0.0 6 783 2 0 0 0 1 1 179 866 0.0 6 858 7 0 0 0 1 180 628 0.0 6 621 5 0 1 0 0 1 181 630 0.0 6 625 5 182 499 0.0 6 495 4 183 460 0.0 6 457 1 1 0 1 184 406 0.0 6 402 2 1 0 0 0 1 185 437 0.0 6 434 2 0 1 186 631 0.0 6 627 4 187 731 0.0 6 721 10 188 685 0.0 6 676 7 2 189 532 0.0 6 528 4 190 527 0.0 6 523 4 191 451 0.0 6 445 5 1 192 418 0.0 6 416 2 193 395 0.0 6 391 3 1 194 376 0.0 6 374 2 195 313 0.0 6 310 1 1 1 196 300 0.0 6 297 3 197 258 0.0 6 256 2 198 288 0.0 6 283 4 1 199 289 0.0 6 287 2 200 268 0.0 6 263 4 0 1 201 262 0.0 6 259 3 202 182 0.0 6 181 1 203 199 0.0 6 198 1 204 149 0.0 6 148 0 1 205 177 0.0 6 176 0 1 206 138 0.0 6 133 3 0 0 1 0 1 207 135 0.0 6 135 208 124 0.0 6 122 1 0 0 1 209 88 0.0 6 85 3 210 95 0.0 6 95 211 97 0.0 6 95 2 212 84 0.0 6 81 3 213 50 0.0 6 50 214 97 0.0 6 97 215 87 0.0 6 85 2 216 80 0.0 6 78 2 217 79 0.0 6 79 218 52 0.0 6 52 219 69 0.0 6 67 1 0 0 0 0 1 220 49 0.0 6 48 0 0 0 1 221 33 0.0 6 33 222 27 0.0 6 26 0 0 0 0 1 223 29 0.0 6 28 1 224 37 0.0 6 37 225 31 0.0 6 30 1 226 39 0.0 6 38 0 0 0 0 1 227 41 0.0 6 39 2 228 38 0.0 6 37 0 0 0 1 229 43 0.0 6 42 1 230 74 0.0 6 70 4 231 39 0.0 6 37 2 232 75 0.0 6 72 3 233 13 0.0 6 11 2 234 32 0.0 6 32 235 22 0.0 6 22 236 15 0.0 6 14 1 237 13 0.0 6 13 238 12 0.0 6 12 239 4 0.0 6 4 240 4 0.0 6 4 241 11 0.0 6 11 242 3 0.0 6 2 1 243 1 0.0 6 1 244 7 0.0 6 7 245 13 0.0 6 13 246 6 0.0 6 5 1 247 9 0.0 6 8 1 248 4 0.0 6 4 249 10 0.0 6 10 250 3 0.0 6 3 251 20 0.0 6 18 2 252 7 0.0 6 7 253 2 0.0 6 2 254 14 0.0 6 14 255 6 0.0 6 6 256 2 0.0 6 2 278 1 0.0 6 1 280 2 0.0 6 2 281 12 0.0 6 12 282 55 0.0 6 52 2 0 0 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1657 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 57 27 0.0 6 27 62 2 0.0 6 0 1 1 63 102 0.0 6 100 2 64 1 0.0 6 1 67 3 0.0 6 2 0 0 0 1 68 1 0.0 6 1 69 8 0.0 6 7 1 70 7 0.0 6 7 71 11 0.0 6 11 72 15 0.0 6 15 73 14 0.0 6 14 74 15 0.0 6 14 0 0 1 75 23 0.0 6 23 76 11 0.0 6 11 77 19 0.0 6 19 78 16 0.0 6 16 79 21 0.0 6 21 80 11 0.0 6 11 81 4 0.0 6 4 82 14 0.0 6 14 83 8 0.0 6 8 84 8 0.0 6 7 1 85 8 0.0 6 8 86 7 0.0 6 7 87 10 0.0 6 10 88 13 0.0 6 13 89 15 0.0 6 15 90 84 0.0 6 84 91 9 0.0 6 9 92 19 0.0 6 18 0 0 0 1 93 6 0.0 6 6 94 13 0.0 6 13 95 12 0.0 6 12 96 9 0.0 6 9 97 5 0.0 6 5 98 11 0.0 6 11 99 11 0.0 6 11 100 9 0.0 6 9 101 15 0.0 6 15 102 14 0.0 6 14 103 6 0.0 6 6 104 16 0.0 6 16 105 5 0.0 6 5 106 11 0.0 6 11 107 26 0.0 6 26 108 7 0.0 6 7 109 14 0.0 6 14 110 5 0.0 6 5 111 6 0.0 6 6 112 9 0.0 6 9 113 10 0.0 6 10 114 5 0.0 6 5 115 4 0.0 6 4 116 9 0.0 6 9 117 7 0.0 6 7 118 8 0.0 6 8 119 19 0.0 6 19 120 6 0.0 6 6 121 12 0.0 6 12 122 18 0.0 6 18 123 12 0.0 6 12 124 5 0.0 6 5 125 11 0.0 6 11 126 6 0.0 6 6 127 2 0.0 6 2 128 11 0.0 6 11 129 13 0.0 6 13 130 19 0.0 6 18 0 0 0 1 131 24 0.0 6 24 132 12 0.0 6 12 133 19 0.0 6 19 134 18 0.0 6 18 135 12 0.0 6 11 0 0 0 1 136 21 0.0 6 21 137 9 0.0 6 8 0 0 0 1 138 12 0.0 6 12 139 7 0.0 6 7 140 16 0.0 6 16 141 8 0.0 6 8 142 9 0.0 6 9 143 9 0.0 6 9 144 10 0.0 6 10 145 13 0.0 6 13 146 17 0.0 6 17 147 12 0.0 6 12 148 19 0.0 6 19 149 38 0.0 6 38 150 11 0.0 6 11 151 15 0.0 6 15 152 20 0.0 6 20 153 23 0.0 6 23 154 13 0.0 6 13 155 19 0.0 6 19 156 7 0.0 6 7 157 9 0.0 6 9 158 17 0.0 6 17 159 12 0.0 6 12 160 17 0.0 6 17 161 16 0.0 6 16 162 11 0.0 6 11 163 9 0.0 6 9 164 18 0.0 6 18 165 5 0.0 6 5 166 3 0.0 6 3 167 9 0.0 6 9 168 12 0.0 6 12 169 7 0.0 6 7 170 8 0.0 6 6 2 171 12 0.0 6 12 172 8 0.0 6 8 173 12 0.0 6 12 174 9 0.0 6 8 0 0 1 175 8 0.0 6 8 176 7 0.0 6 7 177 6 0.0 6 6 178 4 0.0 6 4 179 9 0.0 6 9 180 13 0.0 6 13 181 2 0.0 6 2 182 9 0.0 6 9 183 4 0.0 6 4 184 7 0.0 6 7 185 7 0.0 6 6 0 0 1 186 10 0.0 6 10 187 9 0.0 6 9 188 4 0.0 6 4 189 6 0.0 6 4 0 1 0 1 190 6 0.0 6 6 191 4 0.0 6 3 0 0 1 192 2 0.0 6 2 193 2 0.0 6 1 0 0 1 194 4 0.0 6 4 195 5 0.0 6 5 197 1 0.0 6 1 199 4 0.0 6 4 200 11 0.0 6 11 201 1 0.0 6 1 202 3 0.0 6 3 203 1 0.0 6 1 204 6 0.0 6 6 205 3 0.0 6 3 206 1 0.0 6 1 208 2 0.0 6 2 209 2 0.0 6 2 210 1 0.0 6 1 211 1 0.0 6 1 212 2 0.0 6 2 213 1 0.0 6 1 217 1 0.0 6 1 218 1 0.0 6 1 223 1 0.0 6 1 224 2 0.0 6 2 227 1 0.0 6 1 230 2 0.0 6 2 233 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_10_147_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_10_374_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_10_147_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_10_374_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.57 s (74 µs/read; 0.81 M reads/minute). === Summary === Total read pairs processed: 48,151 Read 1 with adapter: 48,148 (100.0%) Read 2 with adapter: 48,131 (100.0%) == Read fate breakdown == Pairs that were too short: 25 (0.1%) Pairs discarded as untrimmed: 23 (0.0%) Pairs written (passing filters): 48,103 (99.9%) Total basepairs processed: 19,983,839 bp Read 1: 11,140,835 bp Read 2: 8,843,004 bp Total written (filtered): 3,251,865 bp (16.3%) Read 1: 1,658,065 bp Read 2: 1,593,800 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 46761 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 3 188.1 1 1 2 6 3 11.8 1 0 0 3 7 2 2.9 2 0 0 2 10 1 0.0 3 1 13 1 0.0 3 1 14 1 0.0 4 1 16 2 0.0 4 2 17 1 0.0 5 1 18 3 0.0 5 3 20 46 0.0 6 45 1 21 36 0.0 6 34 2 22 27 0.0 6 27 23 19 0.0 6 19 24 23 0.0 6 23 25 11 0.0 6 10 1 26 13 0.0 6 11 2 27 8 0.0 6 8 28 7 0.0 6 6 0 1 29 4 0.0 6 4 30 6 0.0 6 6 31 6 0.0 6 6 32 4 0.0 6 4 33 3 0.0 6 3 34 5 0.0 6 5 35 4 0.0 6 4 36 2 0.0 6 2 37 4 0.0 6 4 38 2 0.0 6 2 39 6 0.0 6 6 40 5 0.0 6 5 41 5 0.0 6 5 42 3 0.0 6 3 43 1 0.0 6 1 44 4 0.0 6 4 45 4 0.0 6 4 46 7 0.0 6 7 47 2 0.0 6 2 48 4 0.0 6 4 49 5 0.0 6 4 0 1 50 3 0.0 6 3 51 3 0.0 6 3 52 4 0.0 6 3 1 53 7 0.0 6 7 54 4 0.0 6 4 55 8 0.0 6 7 1 56 3 0.0 6 3 57 9 0.0 6 9 58 3 0.0 6 3 59 6 0.0 6 6 60 6 0.0 6 6 61 4 0.0 6 4 62 5 0.0 6 5 63 12 0.0 6 10 2 64 6 0.0 6 6 65 3 0.0 6 3 66 4 0.0 6 4 67 3 0.0 6 3 68 8 0.0 6 8 69 10 0.0 6 10 70 5 0.0 6 5 71 10 0.0 6 10 72 11 0.0 6 11 73 8 0.0 6 8 74 10 0.0 6 10 75 15 0.0 6 15 76 4 0.0 6 4 77 7 0.0 6 6 1 78 9 0.0 6 9 79 8 0.0 6 7 1 80 5 0.0 6 5 81 11 0.0 6 11 82 10 0.0 6 10 83 8 0.0 6 8 84 10 0.0 6 9 1 85 11 0.0 6 11 86 6 0.0 6 5 1 87 9 0.0 6 9 88 10 0.0 6 8 2 89 9 0.0 6 9 90 4 0.0 6 4 91 9 0.0 6 7 0 1 1 92 2 0.0 6 2 93 7 0.0 6 7 94 8 0.0 6 7 1 95 8 0.0 6 6 2 96 7 0.0 6 7 97 11 0.0 6 11 98 4 0.0 6 4 99 11 0.0 6 10 1 100 15 0.0 6 14 1 101 9 0.0 6 9 102 18 0.0 6 18 103 10 0.0 6 10 104 16 0.0 6 16 105 13 0.0 6 13 106 7 0.0 6 7 107 10 0.0 6 10 108 14 0.0 6 13 1 109 21 0.0 6 21 110 22 0.0 6 22 111 18 0.0 6 17 1 112 20 0.0 6 20 113 16 0.0 6 14 2 114 16 0.0 6 16 115 18 0.0 6 17 1 116 23 0.0 6 23 117 15 0.0 6 13 1 1 118 24 0.0 6 24 119 12 0.0 6 12 120 27 0.0 6 27 121 20 0.0 6 20 122 32 0.0 6 30 2 123 25 0.0 6 24 1 124 21 0.0 6 21 125 18 0.0 6 18 126 24 0.0 6 23 1 127 30 0.0 6 30 128 28 0.0 6 28 129 28 0.0 6 28 130 43 0.0 6 43 131 31 0.0 6 31 132 34 0.0 6 34 133 44 0.0 6 44 134 37 0.0 6 37 135 36 0.0 6 36 136 26 0.0 6 26 137 41 0.0 6 40 1 138 47 0.0 6 47 139 69 0.0 6 67 1 1 140 288 0.0 6 276 12 141 70 0.0 6 68 2 142 82 0.0 6 82 143 180 0.0 6 179 1 144 254 0.0 6 254 145 327 0.0 6 325 2 146 362 0.0 6 362 147 353 0.0 6 349 4 148 489 0.0 6 488 1 149 498 0.0 6 495 3 150 451 0.0 6 450 1 151 329 0.0 6 328 1 152 483 0.0 6 482 1 153 790 0.0 6 787 3 154 417 0.0 6 417 155 270 0.0 6 268 2 156 367 0.0 6 366 1 157 258 0.0 6 255 3 158 293 0.0 6 291 1 1 159 268 0.0 6 266 2 160 280 0.0 6 279 0 0 0 1 161 383 0.0 6 383 162 331 0.0 6 329 2 163 329 0.0 6 329 164 306 0.0 6 306 165 361 0.0 6 361 166 271 0.0 6 271 167 335 0.0 6 335 168 182 0.0 6 181 1 169 249 0.0 6 249 170 277 0.0 6 274 3 171 336 0.0 6 334 2 172 352 0.0 6 349 2 0 0 0 0 1 173 339 0.0 6 339 174 297 0.0 6 297 175 282 0.0 6 282 176 237 0.0 6 237 177 212 0.0 6 212 178 228 0.0 6 226 2 179 185 0.0 6 184 1 180 167 0.0 6 167 181 430 0.0 6 429 1 182 266 0.0 6 264 2 183 224 0.0 6 222 1 0 0 1 184 384 0.0 6 383 1 185 325 0.0 6 319 4 0 0 0 1 1 186 428 0.0 6 426 2 187 340 0.0 6 338 1 0 0 0 0 1 188 308 0.0 6 307 1 189 316 0.0 6 314 1 0 0 0 0 1 190 389 0.0 6 387 2 191 570 0.0 6 569 0 0 0 0 1 192 370 0.0 6 368 2 193 375 0.0 6 374 1 194 450 0.0 6 447 2 0 0 0 0 1 195 582 0.0 6 578 4 196 546 0.0 6 542 3 0 0 0 1 197 581 0.0 6 578 3 198 477 0.0 6 473 2 1 1 199 583 0.0 6 582 1 200 489 0.0 6 488 1 201 378 0.0 6 374 3 0 0 0 1 202 434 0.0 6 430 4 203 494 0.0 6 493 1 204 493 0.0 6 491 2 205 743 0.0 6 739 4 206 478 0.0 6 476 1 0 1 207 544 0.0 6 538 5 0 1 208 565 0.0 6 563 2 209 390 0.0 6 387 3 210 501 0.0 6 496 4 0 0 1 211 567 0.0 6 561 4 1 0 1 212 490 0.0 6 486 4 213 623 0.0 6 619 2 0 0 0 1 1 214 675 0.0 6 671 4 215 508 0.0 6 505 1 1 0 1 216 596 0.0 6 588 6 1 0 0 1 217 565 0.0 6 563 2 218 502 0.0 6 496 5 1 219 471 0.0 6 469 2 220 383 0.0 6 381 1 1 221 452 0.0 6 450 2 222 480 0.0 6 479 1 223 420 0.0 6 417 2 0 1 224 469 0.0 6 463 4 1 0 0 1 225 460 0.0 6 458 2 226 409 0.0 6 406 3 227 355 0.0 6 353 1 0 0 1 228 346 0.0 6 345 1 229 333 0.0 6 330 2 0 1 230 352 0.0 6 350 2 231 363 0.0 6 360 2 0 0 1 232 475 0.0 6 474 1 233 317 0.0 6 314 2 0 0 1 234 311 0.0 6 307 3 0 0 0 1 235 260 0.0 6 260 236 293 0.0 6 293 237 308 0.0 6 307 0 0 0 1 238 346 0.0 6 345 1 239 305 0.0 6 304 1 240 278 0.0 6 277 0 0 0 0 1 241 280 0.0 6 280 242 232 0.0 6 231 0 1 243 211 0.0 6 210 1 244 235 0.0 6 234 1 245 165 0.0 6 161 3 0 0 0 0 1 246 144 0.0 6 142 2 247 180 0.0 6 180 248 162 0.0 6 160 2 249 172 0.0 6 172 250 170 0.0 6 170 251 127 0.0 6 127 252 144 0.0 6 144 253 112 0.0 6 110 1 1 254 126 0.0 6 126 255 140 0.0 6 137 3 256 137 0.0 6 134 2 0 0 0 1 257 151 0.0 6 149 2 258 136 0.0 6 135 1 259 150 0.0 6 150 260 96 0.0 6 95 1 261 91 0.0 6 91 262 101 0.0 6 100 1 263 127 0.0 6 125 2 264 100 0.0 6 100 265 67 0.0 6 65 2 266 70 0.0 6 69 1 267 65 0.0 6 65 268 92 0.0 6 92 269 56 0.0 6 55 1 270 59 0.0 6 58 1 271 42 0.0 6 42 272 41 0.0 6 41 273 82 0.0 6 80 2 274 82 0.0 6 80 2 275 129 0.0 6 129 276 172 0.0 6 170 2 277 533 0.0 6 529 3 0 0 1 278 998 0.0 6 984 13 1 279 18 0.0 6 18 280 3 0.0 6 3 281 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1387 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 1 0.0 6 0 0 0 0 1 63 5 0.0 6 4 1 64 181 0.0 6 179 2 65 2 0.0 6 2 72 1 0.0 6 1 73 8 0.0 6 8 74 10 0.0 6 10 75 18 0.0 6 18 76 7 0.0 6 7 77 13 0.0 6 13 78 16 0.0 6 16 79 26 0.0 6 26 80 8 0.0 6 8 81 6 0.0 6 6 82 16 0.0 6 16 83 38 0.0 6 38 84 10 0.0 6 10 85 9 0.0 6 9 86 10 0.0 6 10 87 10 0.0 6 10 88 9 0.0 6 9 89 9 0.0 6 9 90 14 0.0 6 14 91 10 0.0 6 10 92 7 0.0 6 7 93 8 0.0 6 8 94 8 0.0 6 8 95 12 0.0 6 12 96 7 0.0 6 7 97 9 0.0 6 9 98 5 0.0 6 5 99 7 0.0 6 7 100 12 0.0 6 12 101 8 0.0 6 8 102 5 0.0 6 5 103 7 0.0 6 7 104 4 0.0 6 4 105 3 0.0 6 3 106 6 0.0 6 6 107 10 0.0 6 10 108 4 0.0 6 4 109 4 0.0 6 4 110 8 0.0 6 7 1 111 12 0.0 6 12 112 5 0.0 6 5 113 4 0.0 6 4 114 7 0.0 6 7 115 14 0.0 6 14 116 9 0.0 6 9 117 9 0.0 6 9 118 7 0.0 6 7 119 5 0.0 6 5 120 12 0.0 6 11 1 121 12 0.0 6 12 122 8 0.0 6 8 123 4 0.0 6 4 124 8 0.0 6 8 125 8 0.0 6 8 126 2 0.0 6 2 127 3 0.0 6 3 128 8 0.0 6 8 129 10 0.0 6 10 130 8 0.0 6 8 131 9 0.0 6 9 132 6 0.0 6 6 133 6 0.0 6 6 134 8 0.0 6 8 135 19 0.0 6 19 136 3 0.0 6 3 137 12 0.0 6 12 138 12 0.0 6 12 139 5 0.0 6 5 140 7 0.0 6 7 141 1 0.0 6 1 142 2 0.0 6 2 143 8 0.0 6 8 144 4 0.0 6 4 145 5 0.0 6 5 146 6 0.0 6 6 147 3 0.0 6 3 148 4 0.0 6 4 149 12 0.0 6 12 150 6 0.0 6 5 0 1 151 8 0.0 6 8 152 4 0.0 6 4 154 2 0.0 6 2 155 4 0.0 6 4 156 9 0.0 6 9 157 2 0.0 6 2 158 7 0.0 6 7 159 10 0.0 6 10 160 7 0.0 6 7 161 3 0.0 6 3 162 8 0.0 6 8 163 5 0.0 6 5 164 12 0.0 6 12 165 3 0.0 6 3 166 4 0.0 6 4 167 6 0.0 6 6 168 5 0.0 6 5 169 8 0.0 6 8 170 8 0.0 6 8 171 12 0.0 6 12 172 3 0.0 6 3 173 6 0.0 6 6 174 6 0.0 6 6 175 7 0.0 6 7 176 3 0.0 6 3 177 4 0.0 6 4 178 4 0.0 6 4 179 11 0.0 6 11 180 8 0.0 6 8 181 11 0.0 6 11 182 9 0.0 6 9 183 5 0.0 6 5 184 4 0.0 6 4 185 6 0.0 6 6 186 9 0.0 6 9 187 4 0.0 6 4 188 12 0.0 6 12 189 5 0.0 6 5 190 6 0.0 6 6 191 6 0.0 6 6 192 5 0.0 6 5 193 5 0.0 6 5 194 6 0.0 6 6 195 10 0.0 6 10 196 7 0.0 6 7 197 10 0.0 6 10 198 5 0.0 6 5 199 1 0.0 6 1 200 5 0.0 6 5 201 4 0.0 6 4 202 1 0.0 6 1 203 2 0.0 6 2 204 6 0.0 6 6 205 6 0.0 6 6 207 7 0.0 6 7 208 6 0.0 6 6 209 6 0.0 6 6 210 7 0.0 6 7 211 7 0.0 6 6 1 212 2 0.0 6 2 213 9 0.0 6 9 214 2 0.0 6 2 215 6 0.0 6 6 216 2 0.0 6 2 217 4 0.0 6 4 218 7 0.0 6 7 219 5 0.0 6 5 220 2 0.0 6 2 221 4 0.0 6 4 222 7 0.0 6 7 223 8 0.0 6 8 224 1 0.0 6 1 225 4 0.0 6 4 226 8 0.0 6 8 227 1 0.0 6 1 228 4 0.0 6 4 229 1 0.0 6 1 230 5 0.0 6 5 231 2 0.0 6 2 232 1 0.0 6 1 233 5 0.0 6 5 234 3 0.0 6 3 235 1 0.0 6 1 237 2 0.0 6 2 238 2 0.0 6 2 240 4 0.0 6 4 241 1 0.0 6 1 242 2 0.0 6 2 243 2 0.0 6 2 244 1 0.0 6 1 245 1 0.0 6 1 246 3 0.0 6 3 248 2 0.0 6 2 249 5 0.0 6 5 250 4 0.0 6 4 251 1 0.0 6 1 254 2 0.0 6 2 255 2 0.0 6 2 257 1 0.0 6 1 258 2 0.0 6 2 259 2 0.0 6 2 260 2 0.0 6 2 261 2 0.0 6 2 262 2 0.0 6 2 263 1 0.0 6 1 265 3 0.0 6 3 266 2 0.0 6 2 267 1 0.0 6 1 268 3 0.0 6 3 269 3 0.0 6 3 272 2 0.0 6 2 273 1 0.0 6 1 277 1 0.0 6 1 278 1 0.0 6 1 284 1 0.0 6 1 291 1 0.0 6 1 294 1 0.0 6 1 307 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 46666 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 752.4 0 0 2 4 1 188.1 1 1 5 1 47.0 1 1 6 1 11.8 1 1 7 2 2.9 2 2 10 1 0.0 3 1 11 1 0.0 3 1 13 8 0.0 3 7 1 14 7 0.0 4 6 1 16 6 0.0 4 6 17 15 0.0 5 13 1 1 18 22 0.0 5 15 6 1 19 12 0.0 5 10 2 20 63 0.0 6 60 3 21 40 0.0 6 39 1 22 20 0.0 6 18 2 23 28 0.0 6 26 2 24 20 0.0 6 18 2 25 19 0.0 6 18 1 26 18 0.0 6 16 2 27 12 0.0 6 11 1 28 18 0.0 6 18 29 12 0.0 6 9 3 30 12 0.0 6 12 31 12 0.0 6 12 32 15 0.0 6 14 1 33 9 0.0 6 9 34 6 0.0 6 5 1 35 9 0.0 6 8 1 36 15 0.0 6 11 2 1 0 0 1 37 9 0.0 6 8 1 38 6 0.0 6 6 39 8 0.0 6 8 40 14 0.0 6 12 2 41 16 0.0 6 16 42 14 0.0 6 11 3 43 12 0.0 6 10 0 0 0 2 44 10 0.0 6 10 45 12 0.0 6 12 46 8 0.0 6 8 47 10 0.0 6 9 1 48 15 0.0 6 13 2 49 18 0.0 6 15 3 50 18 0.0 6 16 1 1 51 27 0.0 6 25 2 52 27 0.0 6 22 4 0 0 0 1 53 24 0.0 6 22 1 1 54 17 0.0 6 14 3 55 18 0.0 6 17 0 0 0 0 0 1 56 16 0.0 6 15 1 57 19 0.0 6 19 58 24 0.0 6 20 4 59 15 0.0 6 12 2 1 60 17 0.0 6 14 2 0 1 61 14 0.0 6 10 4 62 24 0.0 6 21 3 63 38 0.0 6 37 1 64 32 0.0 6 30 1 1 65 24 0.0 6 23 1 66 46 0.0 6 45 1 67 98 0.0 6 93 4 0 0 0 1 68 38 0.0 6 37 1 69 61 0.0 6 56 3 0 0 2 70 37 0.0 6 32 4 0 1 71 50 0.0 6 46 3 0 1 72 26 0.0 6 24 1 1 73 26 0.0 6 24 1 1 74 34 0.0 6 29 5 75 40 0.0 6 40 76 32 0.0 6 32 77 31 0.0 6 29 1 0 0 0 1 78 28 0.0 6 25 2 0 1 79 44 0.0 6 43 1 80 39 0.0 6 36 2 1 81 42 0.0 6 42 82 57 0.0 6 53 4 83 71 0.0 6 70 1 84 50 0.0 6 48 2 85 39 0.0 6 38 1 86 41 0.0 6 38 3 87 34 0.0 6 32 2 88 27 0.0 6 27 89 48 0.0 6 46 2 90 54 0.0 6 51 1 0 0 1 1 91 38 0.0 6 35 1 0 1 0 0 1 92 52 0.0 6 49 2 0 0 0 0 1 93 64 0.0 6 62 2 94 55 0.0 6 55 95 71 0.0 6 70 0 0 0 0 1 96 85 0.0 6 81 4 97 78 0.0 6 75 3 98 79 0.0 6 79 99 71 0.0 6 67 4 100 78 0.0 6 70 6 0 1 0 0 1 101 80 0.0 6 76 4 102 71 0.0 6 69 1 0 0 0 1 103 103 0.0 6 98 4 1 104 133 0.0 6 131 0 0 1 0 0 1 105 79 0.0 6 76 3 106 82 0.0 6 80 2 107 91 0.0 6 86 4 0 0 1 108 90 0.0 6 88 2 109 102 0.0 6 100 2 110 114 0.0 6 112 2 111 100 0.0 6 99 0 1 112 143 0.0 6 140 2 0 0 0 1 113 143 0.0 6 136 6 0 0 0 1 114 131 0.0 6 127 4 115 120 0.0 6 118 2 116 113 0.0 6 110 3 117 161 0.0 6 155 6 118 179 0.0 6 173 6 119 177 0.0 6 170 5 0 0 0 1 1 120 144 0.0 6 143 1 121 168 0.0 6 163 3 0 1 1 122 143 0.0 6 140 3 123 153 0.0 6 148 4 0 1 124 195 0.0 6 191 4 125 256 0.0 6 248 8 126 197 0.0 6 194 3 127 242 0.0 6 237 5 128 309 0.0 6 305 4 129 285 0.0 6 281 4 130 311 0.0 6 307 4 131 359 0.0 6 350 9 132 412 0.0 6 402 6 1 1 1 1 133 414 0.0 6 405 8 1 134 413 0.0 6 404 4 0 3 1 0 1 135 385 0.0 6 378 7 136 397 0.0 6 387 9 0 0 0 0 1 137 370 0.0 6 362 7 0 0 1 138 350 0.0 6 342 5 0 0 0 0 3 139 435 0.0 6 424 10 0 0 0 1 140 488 0.0 6 483 3 1 1 141 598 0.0 6 594 4 142 722 0.0 6 710 11 0 0 1 143 684 0.0 6 671 10 1 0 0 1 1 144 795 0.0 6 784 11 145 792 0.0 6 777 13 0 1 1 146 891 0.0 6 877 11 0 1 0 0 2 147 956 0.0 6 941 14 1 148 998 0.0 6 990 7 0 0 1 149 1038 0.0 6 1021 15 0 0 2 150 1466 0.0 6 1438 21 1 0 1 3 2 151 1274 0.0 6 1257 16 0 0 1 152 1119 0.0 6 1105 11 1 0 1 0 1 153 1036 0.0 6 1024 11 0 0 1 154 859 0.0 6 847 9 1 2 155 912 0.0 6 898 12 1 1 156 771 0.0 6 760 8 1 2 157 962 0.0 6 950 7 1 2 2 158 831 0.0 6 816 12 1 0 1 1 159 745 0.0 6 738 5 1 0 0 0 1 160 732 0.0 6 723 6 1 1 1 161 725 0.0 6 719 5 0 0 1 162 602 0.0 6 597 5 163 678 0.0 6 673 5 164 582 0.0 6 577 5 165 575 0.0 6 567 8 166 696 0.0 6 689 6 0 0 0 1 167 596 0.0 6 584 10 0 1 1 168 651 0.0 6 646 5 169 592 0.0 6 590 2 170 541 0.0 6 537 4 171 597 0.0 6 590 6 0 0 1 172 576 0.0 6 568 8 173 582 0.0 6 576 6 174 490 0.0 6 486 4 175 490 0.0 6 484 6 176 506 0.0 6 497 7 1 0 1 177 421 0.0 6 415 3 0 1 1 1 178 380 0.0 6 377 3 179 331 0.0 6 328 2 0 1 180 313 0.0 6 310 3 181 267 0.0 6 267 182 293 0.0 6 291 2 183 382 0.0 6 376 5 1 184 556 0.0 6 549 5 0 0 0 1 1 185 484 0.0 6 481 3 186 301 0.0 6 298 3 187 299 0.0 6 297 1 0 0 1 188 311 0.0 6 308 2 0 1 189 258 0.0 6 252 5 1 190 231 0.0 6 230 1 191 243 0.0 6 238 5 192 174 0.0 6 174 193 185 0.0 6 183 2 194 135 0.0 6 133 1 0 0 0 1 195 147 0.0 6 147 196 173 0.0 6 171 2 197 149 0.0 6 149 198 126 0.0 6 125 1 199 115 0.0 6 115 200 102 0.0 6 101 0 0 0 1 201 100 0.0 6 99 1 202 78 0.0 6 77 1 203 83 0.0 6 79 3 1 204 85 0.0 6 83 2 205 58 0.0 6 57 1 206 60 0.0 6 57 1 0 0 1 1 207 52 0.0 6 51 1 208 62 0.0 6 61 1 209 38 0.0 6 37 1 210 40 0.0 6 39 1 211 31 0.0 6 31 212 41 0.0 6 40 1 213 38 0.0 6 37 1 214 47 0.0 6 46 1 215 39 0.0 6 38 1 216 29 0.0 6 27 2 217 30 0.0 6 30 218 29 0.0 6 29 219 32 0.0 6 32 220 24 0.0 6 23 0 0 1 221 28 0.0 6 26 2 222 31 0.0 6 31 223 28 0.0 6 26 1 0 1 224 32 0.0 6 31 1 225 10 0.0 6 10 226 23 0.0 6 23 227 14 0.0 6 14 228 30 0.0 6 30 229 14 0.0 6 14 230 4 0.0 6 3 1 231 13 0.0 6 13 232 5 0.0 6 5 233 2 0.0 6 2 234 3 0.0 6 3 235 7 0.0 6 7 236 1 0.0 6 1 237 2 0.0 6 2 238 1 0.0 6 1 239 2 0.0 6 2 240 5 0.0 6 5 241 3 0.0 6 3 242 1 0.0 6 1 243 1 0.0 6 1 244 1 0.0 6 1 245 1 0.0 6 1 246 1 0.0 6 1 247 1 0.0 6 1 248 3 0.0 6 3 277 3 0.0 6 3 278 6 0.0 6 6 279 43 0.0 6 42 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1465 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 51 1 0.0 6 1 52 2 0.0 6 2 64 1 0.0 6 1 67 1 0.0 6 1 68 9 0.0 6 7 1 1 69 316 0.0 6 312 4 70 1 0.0 6 0 1 71 3 0.0 6 3 72 5 0.0 6 4 0 0 1 73 10 0.0 6 9 0 0 0 1 74 11 0.0 6 10 1 75 12 0.0 6 12 76 7 0.0 6 7 77 24 0.0 6 24 78 26 0.0 6 26 79 23 0.0 6 23 80 4 0.0 6 4 81 32 0.0 6 32 82 28 0.0 6 28 83 11 0.0 6 10 0 0 0 1 84 13 0.0 6 13 85 16 0.0 6 16 86 7 0.0 6 7 87 5 0.0 6 5 88 11 0.0 6 11 89 8 0.0 6 8 90 16 0.0 6 16 91 14 0.0 6 13 0 0 0 1 92 15 0.0 6 15 93 10 0.0 6 10 94 11 0.0 6 11 95 10 0.0 6 10 96 8 0.0 6 8 97 6 0.0 6 6 98 4 0.0 6 4 99 4 0.0 6 4 100 15 0.0 6 15 101 9 0.0 6 9 102 9 0.0 6 9 103 13 0.0 6 13 104 15 0.0 6 15 105 1 0.0 6 1 106 7 0.0 6 7 107 7 0.0 6 7 108 4 0.0 6 4 109 6 0.0 6 6 110 14 0.0 6 14 111 4 0.0 6 4 112 8 0.0 6 8 113 13 0.0 6 13 114 4 0.0 6 4 115 13 0.0 6 13 116 15 0.0 6 12 3 117 6 0.0 6 6 118 2 0.0 6 2 119 11 0.0 6 11 120 12 0.0 6 12 121 4 0.0 6 4 122 5 0.0 6 5 123 9 0.0 6 9 124 14 0.0 6 14 125 5 0.0 6 5 126 8 0.0 6 8 127 12 0.0 6 12 128 17 0.0 6 17 129 8 0.0 6 8 130 5 0.0 6 5 131 7 0.0 6 7 132 15 0.0 6 15 133 7 0.0 6 7 134 16 0.0 6 16 135 7 0.0 6 7 136 6 0.0 6 6 137 28 0.0 6 28 138 6 0.0 6 6 139 3 0.0 6 3 140 5 0.0 6 5 141 6 0.0 6 6 142 9 0.0 6 9 143 6 0.0 6 6 144 5 0.0 6 5 145 4 0.0 6 4 146 3 0.0 6 3 147 3 0.0 6 3 148 9 0.0 6 9 149 7 0.0 6 7 150 5 0.0 6 5 151 19 0.0 6 19 152 6 0.0 6 6 153 2 0.0 6 2 154 4 0.0 6 4 155 9 0.0 6 9 156 5 0.0 6 5 157 8 0.0 6 8 158 5 0.0 6 5 159 5 0.0 6 5 160 3 0.0 6 3 161 8 0.0 6 8 162 11 0.0 6 11 163 22 0.0 6 22 164 5 0.0 6 5 165 8 0.0 6 8 166 12 0.0 6 12 167 7 0.0 6 7 168 6 0.0 6 6 169 13 0.0 6 13 170 13 0.0 6 13 171 4 0.0 6 4 172 4 0.0 6 4 173 14 0.0 6 14 174 10 0.0 6 9 0 0 0 1 175 4 0.0 6 4 176 4 0.0 6 4 177 3 0.0 6 3 178 8 0.0 6 8 179 8 0.0 6 8 180 11 0.0 6 10 1 181 7 0.0 6 7 182 2 0.0 6 2 183 1 0.0 6 1 184 8 0.0 6 8 185 6 0.0 6 6 186 3 0.0 6 3 187 4 0.0 6 3 0 0 1 188 10 0.0 6 10 189 5 0.0 6 5 190 2 0.0 6 2 191 3 0.0 6 3 192 2 0.0 6 2 193 3 0.0 6 3 194 7 0.0 6 7 196 1 0.0 6 1 197 5 0.0 6 5 199 1 0.0 6 1 200 6 0.0 6 6 202 2 0.0 6 2 203 2 0.0 6 2 204 2 0.0 6 2 205 2 0.0 6 2 206 1 0.0 6 1 207 2 0.0 6 2 210 4 0.0 6 4 212 1 0.0 6 1 213 1 0.0 6 1 214 1 0.0 6 1 218 2 0.0 6 2 222 2 0.0 6 2 224 2 0.0 6 2 225 4 0.0 6 4 226 1 0.0 6 1 227 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_11_148_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_11_375_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_11_148_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_11_375_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 6.10 s (127 µs/read; 0.47 M reads/minute). === Summary === Total read pairs processed: 48,013 Read 1 with adapter: 48,008 (100.0%) Read 2 with adapter: 47,998 (100.0%) == Read fate breakdown == Pairs that were too short: 6 (0.0%) Pairs discarded as untrimmed: 19 (0.0%) Pairs written (passing filters): 47,988 (99.9%) Total basepairs processed: 19,326,860 bp Read 1: 10,398,497 bp Read 2: 8,928,363 bp Total written (filtered): 3,143,520 bp (16.3%) Read 1: 1,672,791 bp Read 2: 1,470,729 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 46436 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 750.2 0 1 4 4 187.6 1 3 1 7 3 2.9 2 1 0 2 15 1 0.0 4 0 0 1 16 2 0.0 4 0 2 17 1 0.0 5 1 18 1 0.0 5 1 19 1 0.0 5 1 20 36 0.0 6 34 2 21 42 0.0 6 38 4 22 26 0.0 6 26 23 19 0.0 6 17 2 24 16 0.0 6 15 1 25 8 0.0 6 8 26 11 0.0 6 11 27 9 0.0 6 9 28 7 0.0 6 6 1 29 11 0.0 6 10 0 0 0 1 30 4 0.0 6 3 1 31 6 0.0 6 5 0 1 32 1 0.0 6 1 33 3 0.0 6 3 34 5 0.0 6 5 35 8 0.0 6 8 36 3 0.0 6 3 37 2 0.0 6 2 38 8 0.0 6 8 39 6 0.0 6 5 1 40 2 0.0 6 2 41 10 0.0 6 10 42 2 0.0 6 2 43 6 0.0 6 5 1 44 3 0.0 6 3 45 3 0.0 6 2 1 46 4 0.0 6 4 47 6 0.0 6 6 48 7 0.0 6 6 0 0 0 0 0 1 49 5 0.0 6 5 50 2 0.0 6 2 51 5 0.0 6 5 52 4 0.0 6 4 53 5 0.0 6 5 54 2 0.0 6 2 55 1 0.0 6 1 57 4 0.0 6 4 59 5 0.0 6 4 1 60 5 0.0 6 5 61 3 0.0 6 3 62 6 0.0 6 6 63 1 0.0 6 1 64 1 0.0 6 1 65 2 0.0 6 2 66 9 0.0 6 9 67 6 0.0 6 6 68 5 0.0 6 4 1 69 5 0.0 6 5 70 4 0.0 6 4 71 4 0.0 6 3 1 72 7 0.0 6 6 0 0 1 73 8 0.0 6 8 74 5 0.0 6 5 75 6 0.0 6 6 76 6 0.0 6 5 1 77 10 0.0 6 10 78 6 0.0 6 6 79 3 0.0 6 3 80 7 0.0 6 7 81 8 0.0 6 8 82 6 0.0 6 5 1 83 7 0.0 6 7 84 5 0.0 6 5 85 11 0.0 6 10 1 86 6 0.0 6 6 87 8 0.0 6 8 88 8 0.0 6 8 89 5 0.0 6 5 90 6 0.0 6 6 91 4 0.0 6 4 92 5 0.0 6 5 93 8 0.0 6 8 94 7 0.0 6 7 95 6 0.0 6 6 96 5 0.0 6 5 97 2 0.0 6 1 1 98 5 0.0 6 5 99 14 0.0 6 13 1 100 5 0.0 6 5 101 8 0.0 6 7 1 102 14 0.0 6 14 103 16 0.0 6 16 104 14 0.0 6 14 105 11 0.0 6 11 106 28 0.0 6 28 107 13 0.0 6 13 108 18 0.0 6 18 109 17 0.0 6 17 110 30 0.0 6 29 1 111 21 0.0 6 20 1 112 25 0.0 6 25 113 21 0.0 6 20 1 114 14 0.0 6 14 115 21 0.0 6 21 116 24 0.0 6 22 1 0 0 0 0 1 117 13 0.0 6 13 118 19 0.0 6 18 0 0 0 0 1 119 11 0.0 6 11 120 28 0.0 6 28 121 18 0.0 6 18 122 27 0.0 6 27 123 23 0.0 6 22 1 124 28 0.0 6 28 125 32 0.0 6 32 126 23 0.0 6 23 127 30 0.0 6 30 128 27 0.0 6 26 1 129 38 0.0 6 38 130 47 0.0 6 46 1 131 29 0.0 6 29 132 54 0.0 6 53 1 133 50 0.0 6 49 1 134 53 0.0 6 52 1 135 48 0.0 6 47 0 0 1 136 56 0.0 6 56 137 49 0.0 6 49 138 70 0.0 6 70 139 82 0.0 6 82 140 224 0.0 6 224 141 304 0.0 6 303 1 142 532 0.0 6 531 1 143 965 0.0 6 963 1 1 144 690 0.0 6 687 3 145 998 0.0 6 996 1 0 0 0 1 146 955 0.0 6 953 2 147 889 0.0 6 886 3 148 805 0.0 6 805 149 805 0.0 6 802 3 150 1357 0.0 6 1354 3 151 410 0.0 6 410 152 211 0.0 6 210 1 153 308 0.0 6 307 1 154 399 0.0 6 399 155 215 0.0 6 215 156 357 0.0 6 356 1 157 312 0.0 6 311 1 158 753 0.0 6 753 159 726 0.0 6 726 160 667 0.0 6 665 2 161 287 0.0 6 286 0 1 162 383 0.0 6 381 2 163 343 0.0 6 342 1 164 512 0.0 6 512 165 633 0.0 6 632 1 166 367 0.0 6 365 1 1 167 959 0.0 6 956 3 168 482 0.0 6 479 3 169 1220 0.0 6 1219 1 170 437 0.0 6 437 171 1454 0.0 6 1452 2 172 698 0.0 6 696 1 1 173 580 0.0 6 578 2 174 227 0.0 6 225 2 175 214 0.0 6 214 176 976 0.0 6 975 1 177 166 0.0 6 165 1 178 298 0.0 6 297 1 179 114 0.0 6 111 3 180 165 0.0 6 164 1 181 190 0.0 6 185 5 182 165 0.0 6 165 183 136 0.0 6 136 184 187 0.0 6 187 185 369 0.0 6 367 2 186 141 0.0 6 140 0 0 1 187 108 0.0 6 107 0 0 0 1 188 151 0.0 6 151 189 161 0.0 6 161 190 174 0.0 6 172 1 0 0 0 0 1 191 145 0.0 6 145 192 207 0.0 6 206 1 193 674 0.0 6 673 1 194 352 0.0 6 351 1 195 346 0.0 6 345 1 196 274 0.0 6 274 197 351 0.0 6 349 2 198 323 0.0 6 323 199 319 0.0 6 319 200 810 0.0 6 809 1 201 494 0.0 6 494 202 606 0.0 6 605 1 203 307 0.0 6 306 0 0 1 204 828 0.0 6 826 2 205 434 0.0 6 429 5 206 178 0.0 6 176 2 207 230 0.0 6 228 1 1 208 210 0.0 6 207 3 209 305 0.0 6 305 210 275 0.0 6 273 1 0 0 0 1 211 313 0.0 6 311 2 212 261 0.0 6 261 213 439 0.0 6 438 0 0 1 214 391 0.0 6 389 2 215 438 0.0 6 435 3 216 506 0.0 6 503 3 217 474 0.0 6 472 2 218 335 0.0 6 329 4 0 0 1 1 219 340 0.0 6 338 2 220 244 0.0 6 240 4 221 396 0.0 6 396 222 584 0.0 6 581 3 223 247 0.0 6 245 1 1 224 306 0.0 6 303 2 0 0 0 1 225 317 0.0 6 317 226 278 0.0 6 277 1 227 383 0.0 6 383 228 473 0.0 6 473 229 297 0.0 6 296 1 230 125 0.0 6 125 231 109 0.0 6 109 232 120 0.0 6 117 2 1 233 101 0.0 6 100 1 234 114 0.0 6 113 1 235 169 0.0 6 168 1 236 106 0.0 6 106 237 130 0.0 6 128 2 238 99 0.0 6 99 239 107 0.0 6 106 1 240 130 0.0 6 130 241 128 0.0 6 128 242 155 0.0 6 155 243 75 0.0 6 74 0 0 0 0 1 244 88 0.0 6 88 245 87 0.0 6 87 246 82 0.0 6 82 247 85 0.0 6 85 248 83 0.0 6 83 249 71 0.0 6 70 1 250 87 0.0 6 86 1 251 57 0.0 6 56 1 252 71 0.0 6 71 253 33 0.0 6 33 254 55 0.0 6 55 255 57 0.0 6 56 1 256 56 0.0 6 56 257 56 0.0 6 55 1 258 73 0.0 6 73 259 64 0.0 6 64 260 62 0.0 6 62 261 87 0.0 6 87 262 39 0.0 6 39 263 34 0.0 6 34 264 45 0.0 6 45 265 83 0.0 6 83 266 59 0.0 6 59 267 104 0.0 6 104 268 19 0.0 6 19 269 40 0.0 6 39 0 0 0 0 0 1 270 53 0.0 6 53 271 53 0.0 6 53 272 84 0.0 6 84 273 191 0.0 6 191 274 36 0.0 6 36 275 56 0.0 6 56 276 60 0.0 6 60 277 197 0.0 6 197 278 755 0.0 6 755 279 24 0.0 6 24 280 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1572 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 1 0.0 6 0 1 64 20 0.0 6 20 72 3 0.0 6 3 73 66 0.0 6 64 2 74 7 0.0 6 7 75 29 0.0 6 29 76 36 0.0 6 35 1 77 38 0.0 6 38 78 30 0.0 6 30 79 36 0.0 6 36 80 29 0.0 6 29 81 28 0.0 6 28 82 47 0.0 6 47 83 51 0.0 6 50 0 0 0 1 84 7 0.0 6 7 85 4 0.0 6 4 86 12 0.0 6 12 87 13 0.0 6 13 88 4 0.0 6 4 89 6 0.0 6 6 90 12 0.0 6 12 91 39 0.0 6 39 92 32 0.0 6 31 1 93 20 0.0 6 20 94 9 0.0 6 9 95 14 0.0 6 14 96 11 0.0 6 11 97 13 0.0 6 13 98 23 0.0 6 23 99 11 0.0 6 11 100 34 0.0 6 34 101 17 0.0 6 17 102 46 0.0 6 46 103 11 0.0 6 11 104 53 0.0 6 53 105 20 0.0 6 20 106 14 0.0 6 14 107 5 0.0 6 5 108 10 0.0 6 10 109 34 0.0 6 34 110 7 0.0 6 7 111 11 0.0 6 11 113 4 0.0 6 4 114 10 0.0 6 10 115 3 0.0 6 3 116 2 0.0 6 1 1 117 8 0.0 6 8 118 10 0.0 6 10 119 1 0.0 6 1 121 1 0.0 6 1 122 1 0.0 6 1 123 3 0.0 6 3 124 1 0.0 6 1 125 8 0.0 6 8 126 24 0.0 6 24 127 1 0.0 6 1 128 2 0.0 6 2 131 1 0.0 6 1 132 8 0.0 6 8 133 36 0.0 6 36 134 17 0.0 6 17 135 16 0.0 6 16 136 17 0.0 6 17 137 39 0.0 6 39 138 8 0.0 6 8 139 1 0.0 6 1 141 2 0.0 6 2 142 8 0.0 6 8 143 3 0.0 6 3 144 5 0.0 6 5 145 1 0.0 6 1 146 11 0.0 6 11 147 5 0.0 6 5 148 13 0.0 6 13 149 16 0.0 6 16 150 15 0.0 6 15 151 5 0.0 6 5 152 6 0.0 6 6 153 5 0.0 6 5 154 16 0.0 6 16 155 29 0.0 6 29 158 3 0.0 6 3 159 2 0.0 6 2 160 21 0.0 6 21 161 41 0.0 6 41 163 3 0.0 6 3 164 4 0.0 6 4 165 1 0.0 6 1 166 2 0.0 6 2 167 4 0.0 6 4 168 6 0.0 6 6 169 1 0.0 6 1 170 2 0.0 6 1 1 174 3 0.0 6 3 175 7 0.0 6 7 177 3 0.0 6 3 178 3 0.0 6 3 181 1 0.0 6 1 182 3 0.0 6 3 183 2 0.0 6 2 184 1 0.0 6 1 185 4 0.0 6 4 187 1 0.0 6 1 188 8 0.0 6 8 189 6 0.0 6 6 190 5 0.0 6 5 192 3 0.0 6 3 193 9 0.0 6 9 194 15 0.0 6 15 195 18 0.0 6 18 196 2 0.0 6 2 203 1 0.0 6 1 205 1 0.0 6 1 206 2 0.0 6 2 207 6 0.0 6 6 208 9 0.0 6 9 212 8 0.0 6 8 213 11 0.0 6 11 216 10 0.0 6 10 217 6 0.0 6 6 223 2 0.0 6 2 224 1 0.0 6 1 225 1 0.0 6 1 226 2 0.0 6 2 227 4 0.0 6 4 230 2 0.0 6 2 231 2 0.0 6 2 232 2 0.0 6 2 233 7 0.0 6 7 234 1 0.0 6 1 237 2 0.0 6 2 238 7 0.0 6 7 239 12 0.0 6 12 240 1 0.0 6 1 241 1 0.0 6 1 242 5 0.0 6 5 244 1 0.0 6 1 245 1 0.0 6 1 279 2 0.0 6 2 285 2 0.0 6 2 291 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 46327 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 750.2 0 1 4 1 187.6 1 1 6 2 11.7 1 1 1 7 1 2.9 2 1 10 1 0.0 3 1 12 1 0.0 3 1 13 4 0.0 3 4 14 4 0.0 4 4 16 2 0.0 4 2 17 6 0.0 5 6 19 22 0.0 5 18 4 20 38 0.0 6 38 21 31 0.0 6 30 1 22 16 0.0 6 15 1 23 13 0.0 6 13 24 11 0.0 6 11 25 5 0.0 6 5 26 7 0.0 6 7 27 8 0.0 6 8 28 11 0.0 6 11 29 6 0.0 6 5 1 30 6 0.0 6 5 0 0 0 1 31 7 0.0 6 7 32 8 0.0 6 8 33 4 0.0 6 4 34 5 0.0 6 3 2 35 3 0.0 6 3 36 8 0.0 6 8 37 8 0.0 6 8 38 10 0.0 6 10 39 5 0.0 6 4 0 1 40 7 0.0 6 7 41 7 0.0 6 5 1 0 0 1 42 13 0.0 6 11 2 43 9 0.0 6 9 44 5 0.0 6 4 1 45 8 0.0 6 8 46 5 0.0 6 5 47 8 0.0 6 7 1 48 9 0.0 6 9 49 10 0.0 6 10 50 8 0.0 6 6 2 51 11 0.0 6 10 1 52 11 0.0 6 11 53 20 0.0 6 16 4 54 19 0.0 6 18 1 55 11 0.0 6 11 56 8 0.0 6 8 57 7 0.0 6 5 2 58 12 0.0 6 12 59 12 0.0 6 12 60 9 0.0 6 9 61 11 0.0 6 10 1 62 16 0.0 6 11 3 2 63 13 0.0 6 11 2 64 9 0.0 6 9 65 11 0.0 6 11 66 19 0.0 6 18 1 67 17 0.0 6 15 1 1 68 11 0.0 6 11 69 28 0.0 6 24 1 3 70 77 0.0 6 73 3 1 71 26 0.0 6 24 1 1 72 37 0.0 6 36 1 73 14 0.0 6 11 2 1 74 26 0.0 6 22 1 0 1 0 1 1 75 29 0.0 6 29 76 18 0.0 6 17 1 77 20 0.0 6 19 0 0 0 1 78 26 0.0 6 26 79 30 0.0 6 29 1 80 26 0.0 6 24 2 81 24 0.0 6 23 1 82 30 0.0 6 29 0 0 0 0 0 1 83 31 0.0 6 30 1 84 18 0.0 6 16 2 85 31 0.0 6 30 1 86 50 0.0 6 47 2 0 0 0 1 87 30 0.0 6 30 88 30 0.0 6 30 89 30 0.0 6 28 2 90 23 0.0 6 22 1 91 30 0.0 6 30 92 23 0.0 6 21 2 93 38 0.0 6 37 1 94 36 0.0 6 36 95 35 0.0 6 33 0 2 96 42 0.0 6 42 97 40 0.0 6 40 98 47 0.0 6 46 1 99 47 0.0 6 46 0 1 100 42 0.0 6 39 2 0 0 0 1 101 43 0.0 6 42 1 102 39 0.0 6 39 103 53 0.0 6 53 104 68 0.0 6 65 1 1 1 105 55 0.0 6 52 3 106 69 0.0 6 68 1 107 107 0.0 6 107 108 76 0.0 6 74 2 109 61 0.0 6 60 1 110 59 0.0 6 59 111 59 0.0 6 59 112 71 0.0 6 70 1 113 72 0.0 6 70 1 0 0 1 114 89 0.0 6 87 2 115 119 0.0 6 114 4 1 116 93 0.0 6 91 2 117 89 0.0 6 89 118 100 0.0 6 99 1 119 101 0.0 6 100 1 120 103 0.0 6 102 1 121 130 0.0 6 125 5 122 89 0.0 6 89 123 116 0.0 6 116 124 98 0.0 6 96 0 0 0 1 0 1 125 117 0.0 6 116 0 0 0 0 0 1 126 156 0.0 6 154 2 127 168 0.0 6 168 128 182 0.0 6 180 2 129 178 0.0 6 177 0 0 0 0 0 1 130 196 0.0 6 190 5 0 0 0 0 1 131 191 0.0 6 191 132 275 0.0 6 268 6 0 0 0 0 1 133 239 0.0 6 233 1 0 0 1 2 2 134 267 0.0 6 260 4 0 0 2 0 1 135 229 0.0 6 222 7 136 328 0.0 6 326 2 137 311 0.0 6 309 1 0 0 0 0 1 138 343 0.0 6 340 2 0 0 1 139 373 0.0 6 369 1 0 0 1 0 2 140 380 0.0 6 379 1 141 431 0.0 6 425 4 0 0 1 0 1 142 618 0.0 6 613 3 0 2 143 824 0.0 6 818 6 144 1168 0.0 6 1162 6 145 1088 0.0 6 1083 3 0 0 1 0 1 146 1106 0.0 6 1102 4 147 1244 0.0 6 1236 6 1 0 1 148 1004 0.0 6 999 4 0 0 0 1 149 1079 0.0 6 1074 4 0 0 0 1 150 1157 0.0 6 1151 3 1 1 0 0 1 151 1630 0.0 6 1625 4 0 1 152 823 0.0 6 819 1 0 0 1 0 2 153 973 0.0 6 962 9 1 0 0 1 154 816 0.0 6 804 9 1 0 0 1 1 155 1017 0.0 6 1012 5 156 894 0.0 6 885 5 1 0 1 1 1 157 876 0.0 6 869 3 0 0 4 158 880 0.0 6 875 4 0 1 159 877 0.0 6 873 2 1 0 0 0 1 160 947 0.0 6 939 8 161 859 0.0 6 857 2 162 644 0.0 6 638 4 1 0 0 0 1 163 697 0.0 6 694 3 164 583 0.0 6 583 165 621 0.0 6 618 3 166 605 0.0 6 604 1 167 593 0.0 6 589 4 168 713 0.0 6 711 2 169 846 0.0 6 841 5 170 806 0.0 6 802 4 171 808 0.0 6 804 3 0 1 172 838 0.0 6 835 3 173 583 0.0 6 576 7 174 552 0.0 6 551 1 175 522 0.0 6 517 4 0 0 1 176 490 0.0 6 486 3 0 0 1 177 600 0.0 6 599 1 178 436 0.0 6 434 2 179 410 0.0 6 406 4 180 254 0.0 6 253 1 181 325 0.0 6 323 2 182 253 0.0 6 252 1 183 248 0.0 6 247 1 184 322 0.0 6 322 185 239 0.0 6 239 186 351 0.0 6 351 187 336 0.0 6 334 2 188 333 0.0 6 332 0 0 1 189 275 0.0 6 273 2 190 225 0.0 6 225 191 277 0.0 6 276 0 1 192 238 0.0 6 235 3 193 211 0.0 6 210 1 194 233 0.0 6 233 195 154 0.0 6 154 196 155 0.0 6 155 197 151 0.0 6 151 198 108 0.0 6 108 199 213 0.0 6 212 1 200 155 0.0 6 155 201 208 0.0 6 208 202 112 0.0 6 111 0 0 0 0 1 203 127 0.0 6 127 204 94 0.0 6 94 205 74 0.0 6 74 206 101 0.0 6 101 207 48 0.0 6 48 208 54 0.0 6 54 209 49 0.0 6 49 210 58 0.0 6 58 211 67 0.0 6 67 212 70 0.0 6 70 213 34 0.0 6 34 214 27 0.0 6 27 215 33 0.0 6 33 216 43 0.0 6 43 217 65 0.0 6 65 218 52 0.0 6 52 219 49 0.0 6 49 220 67 0.0 6 67 221 21 0.0 6 21 222 32 0.0 6 32 223 41 0.0 6 41 224 35 0.0 6 35 225 64 0.0 6 64 226 33 0.0 6 33 227 39 0.0 6 39 228 48 0.0 6 48 229 49 0.0 6 49 230 12 0.0 6 12 231 19 0.0 6 19 232 19 0.0 6 19 233 13 0.0 6 13 234 8 0.0 6 8 235 22 0.0 6 22 236 14 0.0 6 14 237 21 0.0 6 21 238 12 0.0 6 12 239 22 0.0 6 22 240 48 0.0 6 48 241 14 0.0 6 14 242 11 0.0 6 11 243 2 0.0 6 2 244 4 0.0 6 4 245 5 0.0 6 5 246 2 0.0 6 2 247 5 0.0 6 5 248 3 0.0 6 3 249 2 0.0 6 2 250 4 0.0 6 4 251 2 0.0 6 2 252 5 0.0 6 5 253 3 0.0 6 3 254 1 0.0 6 1 255 2 0.0 6 2 256 3 0.0 6 3 259 2 0.0 6 2 279 1 0.0 6 1 280 1 0.0 6 1 281 3 0.0 6 3 282 37 0.0 6 35 1 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1671 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 45 1 0.0 6 0 0 1 46 1 0.0 6 0 1 68 2 0.0 6 2 69 2 0.0 6 2 70 14 0.0 6 14 71 26 0.0 6 26 72 38 0.0 6 38 73 29 0.0 6 29 74 47 0.0 6 47 75 58 0.0 6 57 0 0 0 1 76 34 0.0 6 32 1 0 0 1 77 37 0.0 6 36 0 0 0 1 78 37 0.0 6 37 79 72 0.0 6 72 80 16 0.0 6 15 0 0 0 1 81 6 0.0 6 6 82 14 0.0 6 13 0 0 0 1 83 28 0.0 6 27 0 0 0 1 84 12 0.0 6 12 85 7 0.0 6 7 86 17 0.0 6 17 87 37 0.0 6 37 88 28 0.0 6 28 89 32 0.0 6 32 90 9 0.0 6 9 91 17 0.0 6 17 92 16 0.0 6 16 93 16 0.0 6 16 94 35 0.0 6 35 95 26 0.0 6 23 2 1 96 50 0.0 6 50 97 24 0.0 6 24 98 42 0.0 6 42 99 18 0.0 6 18 100 64 0.0 6 64 101 27 0.0 6 26 0 0 0 1 102 29 0.0 6 29 103 7 0.0 6 7 104 9 0.0 6 9 105 45 0.0 6 44 0 0 0 1 106 4 0.0 6 4 107 8 0.0 6 7 0 0 0 1 108 2 0.0 6 2 109 4 0.0 6 4 110 7 0.0 6 7 111 6 0.0 6 6 112 1 0.0 6 1 113 2 0.0 6 2 114 20 0.0 6 20 116 1 0.0 6 1 117 2 0.0 6 2 118 3 0.0 6 3 119 2 0.0 6 2 120 2 0.0 6 2 121 4 0.0 6 4 122 30 0.0 6 30 123 7 0.0 6 7 124 2 0.0 6 2 126 1 0.0 6 1 128 10 0.0 6 10 129 34 0.0 6 34 130 32 0.0 6 32 131 24 0.0 6 24 132 16 0.0 6 16 133 48 0.0 6 48 134 8 0.0 6 7 1 137 3 0.0 6 3 138 8 0.0 6 8 139 6 0.0 6 6 140 5 0.0 6 5 141 5 0.0 6 5 142 22 0.0 6 22 143 2 0.0 6 2 144 13 0.0 6 13 145 10 0.0 6 10 146 18 0.0 6 18 147 2 0.0 6 2 148 10 0.0 6 10 149 2 0.0 6 2 150 15 0.0 6 15 151 26 0.0 6 26 155 3 0.0 6 3 156 20 0.0 6 20 157 36 0.0 6 36 158 1 0.0 6 1 159 2 0.0 6 2 160 5 0.0 6 5 163 4 0.0 6 4 164 25 0.0 6 24 1 165 3 0.0 6 3 166 1 0.0 6 1 168 1 0.0 6 0 0 0 0 1 169 2 0.0 6 2 170 1 0.0 6 1 171 7 0.0 6 7 172 1 0.0 6 1 174 2 0.0 6 2 178 6 0.0 6 6 179 3 0.0 6 3 180 1 0.0 6 1 181 4 0.0 6 4 183 3 0.0 6 3 184 6 0.0 6 6 186 6 0.0 6 6 187 1 0.0 6 0 1 188 2 0.0 6 2 189 7 0.0 6 7 190 1 0.0 6 1 191 16 0.0 6 16 203 2 0.0 6 2 204 7 0.0 6 7 207 1 0.0 6 1 209 1 0.0 6 1 211 1 0.0 6 1 212 2 0.0 6 2 213 7 0.0 6 7 222 4 0.0 6 4 223 1 0.0 6 1 224 1 0.0 6 1 233 1 0.0 6 1 234 4 0.0 6 4 235 13 0.0 6 13 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_12_149_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_12_376_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_12_149_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_12_376_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.77 s (115 µs/read; 0.52 M reads/minute). === Summary === Total read pairs processed: 41,653 Read 1 with adapter: 41,650 (100.0%) Read 2 with adapter: 41,618 (99.9%) == Read fate breakdown == Pairs that were too short: 4 (0.0%) Pairs discarded as untrimmed: 38 (0.1%) Pairs written (passing filters): 41,611 (99.9%) Total basepairs processed: 19,652,643 bp Read 1: 11,075,421 bp Read 2: 8,577,222 bp Total written (filtered): 2,693,243 bp (13.7%) Read 1: 1,389,433 bp Read 2: 1,303,810 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 41357 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 162.7 1 0 1 6 1 10.2 1 0 0 1 7 1 2.5 2 0 0 1 15 1 0.0 4 1 16 1 0.0 4 0 1 19 1 0.0 5 1 20 23 0.0 6 23 21 13 0.0 6 13 22 7 0.0 6 7 23 8 0.0 6 8 24 4 0.0 6 4 25 3 0.0 6 3 26 9 0.0 6 9 27 3 0.0 6 3 28 1 0.0 6 1 31 2 0.0 6 0 1 1 32 1 0.0 6 1 33 4 0.0 6 3 1 34 3 0.0 6 2 1 35 2 0.0 6 2 36 3 0.0 6 3 37 2 0.0 6 2 38 3 0.0 6 2 1 39 1 0.0 6 1 40 1 0.0 6 1 41 2 0.0 6 2 43 1 0.0 6 1 44 3 0.0 6 3 45 1 0.0 6 1 46 3 0.0 6 3 47 2 0.0 6 2 48 1 0.0 6 1 49 4 0.0 6 4 50 1 0.0 6 1 51 3 0.0 6 3 52 3 0.0 6 3 53 4 0.0 6 3 1 54 2 0.0 6 2 55 3 0.0 6 3 56 3 0.0 6 3 57 5 0.0 6 5 58 2 0.0 6 2 59 1 0.0 6 1 60 2 0.0 6 2 62 7 0.0 6 7 63 1 0.0 6 1 64 4 0.0 6 2 2 65 2 0.0 6 2 66 1 0.0 6 1 67 2 0.0 6 2 68 4 0.0 6 4 69 1 0.0 6 1 70 2 0.0 6 2 72 5 0.0 6 5 73 2 0.0 6 2 74 2 0.0 6 2 75 2 0.0 6 2 76 1 0.0 6 1 77 1 0.0 6 1 78 3 0.0 6 3 79 2 0.0 6 2 80 2 0.0 6 2 81 2 0.0 6 2 82 10 0.0 6 9 1 83 5 0.0 6 5 84 4 0.0 6 4 85 3 0.0 6 3 86 4 0.0 6 4 87 4 0.0 6 4 88 1 0.0 6 1 89 8 0.0 6 7 1 90 2 0.0 6 2 91 3 0.0 6 3 92 6 0.0 6 5 0 1 93 6 0.0 6 5 1 94 1 0.0 6 1 95 3 0.0 6 3 96 2 0.0 6 1 1 97 5 0.0 6 4 1 98 7 0.0 6 7 100 5 0.0 6 5 101 5 0.0 6 5 102 5 0.0 6 5 103 8 0.0 6 8 104 4 0.0 6 3 1 105 5 0.0 6 5 106 3 0.0 6 3 107 4 0.0 6 4 108 4 0.0 6 4 109 8 0.0 6 7 0 1 110 8 0.0 6 8 111 9 0.0 6 9 112 10 0.0 6 10 113 13 0.0 6 12 1 114 8 0.0 6 8 115 10 0.0 6 10 116 10 0.0 6 10 117 7 0.0 6 7 118 7 0.0 6 6 1 119 7 0.0 6 7 120 5 0.0 6 5 121 8 0.0 6 8 122 4 0.0 6 4 123 14 0.0 6 14 124 10 0.0 6 10 125 7 0.0 6 7 126 10 0.0 6 10 127 18 0.0 6 18 128 22 0.0 6 22 129 13 0.0 6 13 130 11 0.0 6 11 131 10 0.0 6 9 1 132 25 0.0 6 25 133 14 0.0 6 14 134 19 0.0 6 19 135 21 0.0 6 21 136 18 0.0 6 17 1 137 26 0.0 6 26 138 29 0.0 6 29 139 48 0.0 6 48 140 33 0.0 6 33 141 55 0.0 6 55 142 115 0.0 6 114 1 143 174 0.0 6 174 144 205 0.0 6 205 145 226 0.0 6 226 146 225 0.0 6 225 147 310 0.0 6 310 148 319 0.0 6 319 149 375 0.0 6 375 150 291 0.0 6 290 1 151 325 0.0 6 325 152 399 0.0 6 398 1 153 282 0.0 6 282 154 200 0.0 6 199 1 155 232 0.0 6 231 1 156 177 0.0 6 177 157 186 0.0 6 186 158 151 0.0 6 151 159 201 0.0 6 200 1 160 204 0.0 6 204 161 197 0.0 6 197 162 181 0.0 6 181 163 166 0.0 6 165 1 164 117 0.0 6 117 165 135 0.0 6 134 1 166 137 0.0 6 137 167 85 0.0 6 84 1 168 113 0.0 6 113 169 100 0.0 6 99 1 170 114 0.0 6 113 0 1 171 149 0.0 6 147 2 172 148 0.0 6 148 173 95 0.0 6 94 0 1 174 126 0.0 6 126 175 152 0.0 6 151 1 176 102 0.0 6 102 177 100 0.0 6 100 178 91 0.0 6 90 1 179 82 0.0 6 82 180 183 0.0 6 183 181 113 0.0 6 112 1 182 106 0.0 6 106 183 150 0.0 6 147 2 1 184 97 0.0 6 97 185 93 0.0 6 93 186 68 0.0 6 68 187 114 0.0 6 114 188 98 0.0 6 98 189 125 0.0 6 124 1 190 74 0.0 6 71 3 191 121 0.0 6 121 192 122 0.0 6 117 5 193 164 0.0 6 161 3 194 185 0.0 6 182 3 195 209 0.0 6 209 196 195 0.0 6 193 2 197 175 0.0 6 170 5 198 167 0.0 6 164 2 0 0 1 199 114 0.0 6 112 2 200 105 0.0 6 103 2 201 135 0.0 6 133 2 202 151 0.0 6 151 203 173 0.0 6 171 2 204 204 0.0 6 201 3 205 223 0.0 6 222 1 206 135 0.0 6 133 2 207 202 0.0 6 196 6 208 188 0.0 6 184 4 209 224 0.0 6 217 7 210 190 0.0 6 188 2 211 234 0.0 6 225 8 0 0 1 212 165 0.0 6 164 1 213 179 0.0 6 177 2 214 246 0.0 6 241 5 215 131 0.0 6 130 1 216 221 0.0 6 218 3 217 207 0.0 6 202 3 2 218 166 0.0 6 161 5 219 141 0.0 6 141 220 143 0.0 6 142 1 221 109 0.0 6 108 1 222 195 0.0 6 193 2 223 115 0.0 6 114 1 224 182 0.0 6 176 5 1 225 179 0.0 6 176 3 226 378 0.0 6 373 5 227 251 0.0 6 248 3 228 210 0.0 6 207 3 229 218 0.0 6 214 4 230 222 0.0 6 214 8 231 153 0.0 6 152 1 232 187 0.0 6 183 4 233 162 0.0 6 160 2 234 148 0.0 6 147 1 235 156 0.0 6 155 1 236 127 0.0 6 123 4 237 174 0.0 6 174 238 145 0.0 6 142 3 239 202 0.0 6 199 3 240 262 0.0 6 256 6 241 292 0.0 6 289 3 242 252 0.0 6 248 4 243 232 0.0 6 229 3 244 143 0.0 6 140 3 245 249 0.0 6 245 4 246 145 0.0 6 142 3 247 217 0.0 6 211 6 248 94 0.0 6 92 2 249 226 0.0 6 225 1 250 260 0.0 6 257 2 1 251 193 0.0 6 185 8 252 392 0.0 6 387 5 253 161 0.0 6 157 4 254 251 0.0 6 245 6 255 251 0.0 6 249 2 256 288 0.0 6 282 6 257 409 0.0 6 401 8 258 343 0.0 6 330 13 259 416 0.0 6 409 7 260 252 0.0 6 250 2 261 247 0.0 6 238 9 262 258 0.0 6 248 10 263 444 0.0 6 435 9 264 241 0.0 6 236 5 265 270 0.0 6 264 5 1 266 180 0.0 6 174 6 267 362 0.0 6 356 6 268 296 0.0 6 291 5 269 303 0.0 6 296 7 270 143 0.0 6 138 5 271 344 0.0 6 336 8 272 740 0.0 6 727 12 1 273 496 0.0 6 486 10 274 557 0.0 6 547 8 2 275 322 0.0 6 310 12 276 1390 0.0 6 1361 28 0 1 277 4163 0.0 6 4104 58 1 278 7309 0.0 6 7139 162 5 3 279 193 0.0 6 184 9 280 9 0.0 6 7 1 0 1 281 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 293 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 2 0.0 6 2 64 41 0.0 6 40 1 65 1 0.0 6 1 66 4 0.0 6 4 71 1 0.0 6 1 72 1 0.0 6 1 73 1 0.0 6 1 74 6 0.0 6 6 75 3 0.0 6 3 76 5 0.0 6 5 77 3 0.0 6 3 78 7 0.0 6 7 79 13 0.0 6 13 80 11 0.0 6 11 81 3 0.0 6 3 82 6 0.0 6 6 83 9 0.0 6 9 84 3 0.0 6 3 85 3 0.0 6 3 86 10 0.0 6 10 87 2 0.0 6 2 88 4 0.0 6 4 89 5 0.0 6 5 90 6 0.0 6 6 91 3 0.0 6 3 92 3 0.0 6 3 93 2 0.0 6 2 94 4 0.0 6 4 95 1 0.0 6 1 96 2 0.0 6 2 97 1 0.0 6 1 98 1 0.0 6 1 99 1 0.0 6 1 100 1 0.0 6 1 101 3 0.0 6 3 102 4 0.0 6 4 103 2 0.0 6 2 105 5 0.0 6 5 106 1 0.0 6 1 107 3 0.0 6 3 108 1 0.0 6 1 110 2 0.0 6 2 111 2 0.0 6 2 112 2 0.0 6 2 113 1 0.0 6 1 114 2 0.0 6 2 115 1 0.0 6 1 116 2 0.0 6 2 117 3 0.0 6 3 118 1 0.0 6 1 120 2 0.0 6 2 121 2 0.0 6 2 125 1 0.0 6 1 128 3 0.0 6 3 129 1 0.0 6 1 131 2 0.0 6 2 132 2 0.0 6 2 133 4 0.0 6 4 134 2 0.0 6 2 135 1 0.0 6 1 138 1 0.0 6 1 139 2 0.0 6 2 142 1 0.0 6 1 146 2 0.0 6 2 149 2 0.0 6 2 150 2 0.0 6 2 151 1 0.0 6 1 154 1 0.0 6 0 0 0 0 0 0 1 156 2 0.0 6 2 158 5 0.0 6 5 159 2 0.0 6 2 161 1 0.0 6 1 163 2 0.0 6 2 164 2 0.0 6 2 167 1 0.0 6 1 168 1 0.0 6 1 171 1 0.0 6 1 172 1 0.0 6 1 173 1 0.0 6 1 175 1 0.0 6 1 179 1 0.0 6 1 182 1 0.0 6 1 183 2 0.0 6 2 184 1 0.0 6 1 187 1 0.0 6 1 188 1 0.0 6 1 191 1 0.0 6 1 197 1 0.0 6 1 202 2 0.0 6 2 203 1 0.0 6 1 207 1 0.0 6 1 208 1 0.0 6 1 212 1 0.0 6 1 213 1 0.0 6 1 216 2 0.0 6 2 218 1 0.0 6 1 219 1 0.0 6 1 223 2 0.0 6 2 228 2 0.0 6 2 229 2 0.0 6 2 230 1 0.0 6 1 231 1 0.0 6 1 234 1 0.0 6 1 238 1 0.0 6 1 239 1 0.0 6 1 248 2 0.0 6 2 262 1 0.0 6 1 265 1 0.0 6 1 275 1 0.0 6 1 282 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 41293 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 650.8 0 0 2 5 1 40.7 1 1 8 1 0.6 2 1 10 2 0.0 3 2 11 4 0.0 3 4 12 4 0.0 3 4 13 9 0.0 3 9 14 7 0.0 4 6 0 0 1 15 1 0.0 4 1 16 5 0.0 4 5 17 7 0.0 5 7 18 10 0.0 5 9 1 19 7 0.0 5 7 20 22 0.0 6 22 21 25 0.0 6 23 2 22 20 0.0 6 18 1 1 23 21 0.0 6 18 2 1 24 10 0.0 6 10 25 7 0.0 6 7 26 12 0.0 6 10 2 27 7 0.0 6 7 28 4 0.0 6 3 1 29 6 0.0 6 5 0 1 30 8 0.0 6 8 31 4 0.0 6 3 1 32 5 0.0 6 4 1 33 6 0.0 6 6 34 2 0.0 6 2 35 6 0.0 6 6 36 4 0.0 6 4 37 8 0.0 6 7 1 38 6 0.0 6 6 39 8 0.0 6 7 1 40 4 0.0 6 3 1 41 5 0.0 6 4 1 42 8 0.0 6 8 43 5 0.0 6 5 44 14 0.0 6 12 2 45 11 0.0 6 10 0 0 0 1 46 12 0.0 6 11 1 47 10 0.0 6 9 1 48 12 0.0 6 10 2 49 8 0.0 6 6 2 50 17 0.0 6 17 51 7 0.0 6 7 52 12 0.0 6 12 53 14 0.0 6 13 1 54 17 0.0 6 16 1 55 11 0.0 6 11 56 4 0.0 6 4 57 15 0.0 6 13 2 58 15 0.0 6 14 1 59 17 0.0 6 14 3 60 9 0.0 6 9 61 9 0.0 6 8 1 62 2 0.0 6 2 63 8 0.0 6 8 64 11 0.0 6 10 1 65 6 0.0 6 6 66 12 0.0 6 12 67 32 0.0 6 32 68 73 0.0 6 66 6 1 69 23 0.0 6 23 70 29 0.0 6 28 1 71 16 0.0 6 14 1 0 1 72 26 0.0 6 24 2 73 9 0.0 6 9 74 6 0.0 6 5 1 75 9 0.0 6 9 76 12 0.0 6 11 0 0 0 0 1 77 16 0.0 6 16 78 13 0.0 6 11 2 79 7 0.0 6 7 80 20 0.0 6 18 1 1 81 17 0.0 6 16 1 82 24 0.0 6 21 2 1 83 32 0.0 6 30 2 84 31 0.0 6 31 85 25 0.0 6 24 1 86 12 0.0 6 12 87 22 0.0 6 22 88 27 0.0 6 26 1 89 15 0.0 6 14 1 90 22 0.0 6 20 2 91 14 0.0 6 12 2 92 15 0.0 6 15 93 31 0.0 6 30 1 94 47 0.0 6 43 4 95 30 0.0 6 28 2 96 34 0.0 6 33 1 97 25 0.0 6 22 2 1 98 29 0.0 6 29 99 35 0.0 6 34 1 100 39 0.0 6 37 1 1 101 30 0.0 6 29 1 102 45 0.0 6 43 2 103 50 0.0 6 49 1 104 51 0.0 6 49 2 105 84 0.0 6 81 3 106 47 0.0 6 46 1 107 43 0.0 6 42 1 108 22 0.0 6 22 109 37 0.0 6 35 1 0 0 1 110 44 0.0 6 44 111 42 0.0 6 38 3 0 1 112 36 0.0 6 35 1 113 43 0.0 6 39 4 114 40 0.0 6 39 1 115 47 0.0 6 45 2 116 67 0.0 6 67 117 36 0.0 6 35 1 118 75 0.0 6 74 1 119 61 0.0 6 59 2 120 78 0.0 6 77 1 121 58 0.0 6 56 2 122 66 0.0 6 63 3 123 59 0.0 6 58 1 124 83 0.0 6 79 4 125 86 0.0 6 85 0 1 126 90 0.0 6 90 127 97 0.0 6 95 2 128 79 0.0 6 79 129 94 0.0 6 94 130 78 0.0 6 77 1 131 125 0.0 6 121 4 132 152 0.0 6 148 4 133 135 0.0 6 133 2 134 189 0.0 6 186 3 135 246 0.0 6 239 7 136 129 0.0 6 125 4 137 176 0.0 6 173 2 1 138 128 0.0 6 126 2 139 158 0.0 6 156 2 140 242 0.0 6 234 8 141 264 0.0 6 259 4 0 1 142 369 0.0 6 364 5 143 434 0.0 6 425 7 2 144 463 0.0 6 457 6 145 375 0.0 6 374 1 146 456 0.0 6 451 5 147 385 0.0 6 382 3 148 505 0.0 6 501 3 0 1 149 550 0.0 6 544 6 150 574 0.0 6 573 1 151 669 0.0 6 663 3 2 1 152 530 0.0 6 520 10 153 444 0.0 6 439 4 1 154 524 0.0 6 519 5 155 373 0.0 6 370 3 156 431 0.0 6 425 6 157 379 0.0 6 369 8 0 1 1 158 469 0.0 6 460 7 0 1 0 1 159 379 0.0 6 378 1 160 406 0.0 6 401 5 161 315 0.0 6 311 4 162 265 0.0 6 258 7 163 357 0.0 6 349 8 164 339 0.0 6 332 6 1 165 359 0.0 6 349 7 2 0 1 166 363 0.0 6 358 5 167 350 0.0 6 345 5 168 443 0.0 6 436 7 169 337 0.0 6 332 5 170 282 0.0 6 281 1 171 348 0.0 6 344 3 1 172 287 0.0 6 281 4 2 173 319 0.0 6 313 5 1 174 355 0.0 6 350 5 175 504 0.0 6 493 9 0 0 1 1 176 376 0.0 6 365 10 0 0 1 177 526 0.0 6 512 13 0 0 1 178 349 0.0 6 345 4 179 395 0.0 6 382 12 1 180 368 0.0 6 362 6 181 493 0.0 6 478 15 182 185 0.0 6 182 3 183 466 0.0 6 452 14 184 319 0.0 6 303 15 0 1 185 327 0.0 6 317 10 186 744 0.0 6 714 27 2 0 1 187 463 0.0 6 456 6 0 0 1 188 542 0.0 6 522 18 0 1 0 1 189 577 0.0 6 557 18 1 0 1 190 826 0.0 6 802 24 191 733 0.0 6 711 22 192 546 0.0 6 534 10 2 193 446 0.0 6 434 10 1 0 1 194 583 0.0 6 567 15 1 195 497 0.0 6 482 14 0 1 196 1308 0.0 6 1283 22 1 1 1 197 481 0.0 6 462 18 0 0 0 1 198 793 0.0 6 773 19 0 0 1 199 692 0.0 6 679 12 1 200 372 0.0 6 360 12 201 649 0.0 6 630 18 1 202 309 0.0 6 302 7 203 231 0.0 6 224 7 204 406 0.0 6 398 8 205 238 0.0 6 233 4 1 206 232 0.0 6 224 6 1 0 1 207 225 0.0 6 219 6 208 451 0.0 6 440 11 209 105 0.0 6 103 1 1 210 245 0.0 6 241 4 211 363 0.0 6 355 8 212 385 0.0 6 378 7 213 121 0.0 6 118 3 214 224 0.0 6 218 5 1 215 271 0.0 6 261 9 1 216 240 0.0 6 232 8 217 204 0.0 6 200 4 218 132 0.0 6 127 5 219 166 0.0 6 164 2 220 73 0.0 6 69 4 221 141 0.0 6 138 3 222 157 0.0 6 146 11 223 163 0.0 6 158 5 224 90 0.0 6 83 6 1 225 108 0.0 6 107 1 226 215 0.0 6 209 6 227 303 0.0 6 302 1 228 216 0.0 6 212 3 0 1 229 229 0.0 6 220 8 1 230 613 0.0 6 598 15 231 96 0.0 6 91 5 232 269 0.0 6 264 5 233 107 0.0 6 102 5 234 164 0.0 6 155 7 1 1 235 78 0.0 6 75 3 236 49 0.0 6 48 1 237 46 0.0 6 46 238 42 0.0 6 39 3 239 11 0.0 6 10 1 240 33 0.0 6 33 241 30 0.0 6 30 242 40 0.0 6 39 1 243 29 0.0 6 28 1 244 22 0.0 6 22 245 38 0.0 6 37 0 0 1 246 23 0.0 6 22 1 247 26 0.0 6 25 1 248 20 0.0 6 20 249 53 0.0 6 50 3 250 4 0.0 6 3 1 251 30 0.0 6 30 252 12 0.0 6 11 1 253 16 0.0 6 16 254 64 0.0 6 63 1 255 11 0.0 6 11 256 8 0.0 6 8 257 1 0.0 6 1 258 1 0.0 6 1 278 2 0.0 6 2 279 13 0.0 6 10 3 280 49 0.0 6 49 281 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 325 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 55 1 0.0 6 1 56 1 0.0 6 1 67 3 0.0 6 3 68 28 0.0 6 28 70 1 0.0 6 1 71 5 0.0 6 5 72 7 0.0 6 7 73 4 0.0 6 4 74 6 0.0 6 6 75 4 0.0 6 4 76 11 0.0 6 11 77 4 0.0 6 4 78 14 0.0 6 14 79 6 0.0 6 6 80 8 0.0 6 8 81 12 0.0 6 12 82 10 0.0 6 10 83 4 0.0 6 4 84 4 0.0 6 4 85 3 0.0 6 3 87 4 0.0 6 4 88 5 0.0 6 5 89 11 0.0 6 11 90 3 0.0 6 3 91 3 0.0 6 3 92 5 0.0 6 5 93 4 0.0 6 4 94 6 0.0 6 6 95 1 0.0 6 1 96 3 0.0 6 3 97 3 0.0 6 3 98 2 0.0 6 2 99 4 0.0 6 4 100 1 0.0 6 1 101 5 0.0 6 5 102 2 0.0 6 2 103 2 0.0 6 2 104 5 0.0 6 5 106 1 0.0 6 1 107 2 0.0 6 2 108 4 0.0 6 4 109 3 0.0 6 3 110 1 0.0 6 1 111 3 0.0 6 3 112 3 0.0 6 3 114 1 0.0 6 1 115 2 0.0 6 2 116 1 0.0 6 1 117 3 0.0 6 3 119 1 0.0 6 1 120 2 0.0 6 2 121 2 0.0 6 2 124 3 0.0 6 3 125 1 0.0 6 1 126 2 0.0 6 2 127 1 0.0 6 1 128 3 0.0 6 3 129 3 0.0 6 3 131 1 0.0 6 1 133 4 0.0 6 4 134 1 0.0 6 1 136 1 0.0 6 1 137 2 0.0 6 2 138 1 0.0 6 1 141 2 0.0 6 2 142 3 0.0 6 3 143 1 0.0 6 1 144 2 0.0 6 2 146 1 0.0 6 1 147 1 0.0 6 1 148 3 0.0 6 3 149 1 0.0 6 1 150 1 0.0 6 1 151 1 0.0 6 1 152 1 0.0 6 1 155 3 0.0 6 3 157 6 0.0 6 6 159 5 0.0 6 5 162 3 0.0 6 3 163 4 0.0 6 4 167 1 0.0 6 1 168 1 0.0 6 1 170 2 0.0 6 2 172 1 0.0 6 1 173 1 0.0 6 1 175 1 0.0 6 1 176 2 0.0 6 2 177 3 0.0 6 3 179 1 0.0 6 1 181 1 0.0 6 1 183 2 0.0 6 2 184 1 0.0 6 1 186 1 0.0 6 1 187 2 0.0 6 2 189 1 0.0 6 1 191 1 0.0 6 1 192 1 0.0 6 1 194 1 0.0 6 1 200 1 0.0 6 1 206 1 0.0 6 1 207 1 0.0 6 1 217 1 0.0 6 1 221 1 0.0 6 1 226 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_13_150_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_13_377_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_13_150_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_13_377_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.75 s (109 µs/read; 0.55 M reads/minute). === Summary === Total read pairs processed: 43,558 Read 1 with adapter: 43,555 (100.0%) Read 2 with adapter: 43,536 (99.9%) == Read fate breakdown == Pairs that were too short: 12 (0.0%) Pairs discarded as untrimmed: 25 (0.1%) Pairs written (passing filters): 43,521 (99.9%) Total basepairs processed: 18,476,954 bp Read 1: 10,339,647 bp Read 2: 8,137,307 bp Total written (filtered): 2,822,377 bp (15.3%) Read 1: 1,487,973 bp Read 2: 1,334,404 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 42478 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 680.6 0 1 4 1 170.1 1 1 6 1 10.6 1 0 0 1 7 1 2.7 2 1 13 1 0.0 3 1 15 1 0.0 4 1 16 1 0.0 4 1 17 3 0.0 5 3 18 2 0.0 5 2 19 2 0.0 5 1 1 20 34 0.0 6 34 21 15 0.0 6 14 1 22 15 0.0 6 15 23 14 0.0 6 13 1 24 11 0.0 6 11 25 15 0.0 6 15 26 8 0.0 6 7 0 1 27 10 0.0 6 10 28 11 0.0 6 10 0 0 0 0 0 1 29 3 0.0 6 3 30 3 0.0 6 3 31 2 0.0 6 2 32 3 0.0 6 2 1 33 7 0.0 6 7 34 5 0.0 6 4 1 35 5 0.0 6 5 36 2 0.0 6 2 37 6 0.0 6 5 0 1 38 2 0.0 6 2 39 7 0.0 6 7 40 6 0.0 6 6 41 2 0.0 6 2 42 6 0.0 6 5 0 0 0 0 1 43 1 0.0 6 1 44 4 0.0 6 4 45 2 0.0 6 2 46 4 0.0 6 4 47 5 0.0 6 5 48 3 0.0 6 2 1 49 4 0.0 6 3 1 50 2 0.0 6 2 51 4 0.0 6 4 52 2 0.0 6 2 53 1 0.0 6 1 54 3 0.0 6 3 55 5 0.0 6 5 56 2 0.0 6 2 57 5 0.0 6 4 1 58 3 0.0 6 3 59 4 0.0 6 4 60 2 0.0 6 2 61 4 0.0 6 4 62 1 0.0 6 1 64 5 0.0 6 4 1 65 8 0.0 6 7 1 66 8 0.0 6 8 67 4 0.0 6 4 68 4 0.0 6 4 69 9 0.0 6 9 70 5 0.0 6 5 71 4 0.0 6 3 1 72 3 0.0 6 2 1 73 3 0.0 6 3 74 5 0.0 6 5 75 5 0.0 6 5 76 4 0.0 6 4 79 7 0.0 6 6 0 1 80 4 0.0 6 4 81 7 0.0 6 6 1 82 10 0.0 6 9 1 83 4 0.0 6 4 84 5 0.0 6 5 85 8 0.0 6 8 86 8 0.0 6 8 87 4 0.0 6 4 88 6 0.0 6 6 89 4 0.0 6 4 90 3 0.0 6 3 91 7 0.0 6 7 92 7 0.0 6 6 0 0 0 1 93 12 0.0 6 12 94 4 0.0 6 4 95 4 0.0 6 4 96 4 0.0 6 4 97 3 0.0 6 3 98 9 0.0 6 9 99 9 0.0 6 8 0 1 100 9 0.0 6 9 101 6 0.0 6 6 102 7 0.0 6 6 1 103 10 0.0 6 9 1 104 13 0.0 6 13 105 11 0.0 6 11 106 8 0.0 6 8 107 7 0.0 6 7 108 12 0.0 6 12 109 16 0.0 6 16 110 13 0.0 6 13 111 7 0.0 6 7 112 13 0.0 6 13 113 11 0.0 6 11 114 9 0.0 6 9 115 19 0.0 6 18 1 116 14 0.0 6 14 117 13 0.0 6 13 118 14 0.0 6 14 119 16 0.0 6 16 120 9 0.0 6 9 121 10 0.0 6 9 1 122 20 0.0 6 20 123 17 0.0 6 17 124 16 0.0 6 16 125 17 0.0 6 16 0 0 0 0 1 126 13 0.0 6 11 1 0 0 0 1 127 23 0.0 6 23 128 15 0.0 6 15 129 21 0.0 6 21 130 31 0.0 6 31 131 19 0.0 6 19 132 26 0.0 6 26 133 34 0.0 6 34 134 37 0.0 6 37 135 30 0.0 6 29 0 0 0 1 136 23 0.0 6 21 2 137 31 0.0 6 30 1 138 41 0.0 6 41 139 55 0.0 6 54 1 140 53 0.0 6 52 1 141 187 0.0 6 186 1 142 251 0.0 6 250 1 143 281 0.0 6 281 144 259 0.0 6 259 145 264 0.0 6 263 0 0 0 0 0 1 146 441 0.0 6 441 147 240 0.0 6 240 148 425 0.0 6 424 1 149 286 0.0 6 286 150 312 0.0 6 312 151 573 0.0 6 571 1 0 0 0 1 152 252 0.0 6 251 1 153 225 0.0 6 225 154 171 0.0 6 171 155 212 0.0 6 209 1 0 0 1 0 1 156 227 0.0 6 226 1 157 207 0.0 6 207 158 268 0.0 6 266 2 159 216 0.0 6 216 160 203 0.0 6 202 1 161 263 0.0 6 262 1 162 214 0.0 6 212 1 1 163 237 0.0 6 237 164 247 0.0 6 247 165 275 0.0 6 274 1 166 341 0.0 6 337 3 0 0 1 167 253 0.0 6 252 1 168 209 0.0 6 209 169 291 0.0 6 288 3 170 239 0.0 6 238 1 171 222 0.0 6 220 2 172 177 0.0 6 177 173 294 0.0 6 291 3 174 180 0.0 6 180 175 165 0.0 6 162 3 176 198 0.0 6 198 177 179 0.0 6 179 178 207 0.0 6 207 179 410 0.0 6 408 2 180 307 0.0 6 306 1 181 213 0.0 6 210 2 0 0 1 182 258 0.0 6 258 183 240 0.0 6 239 1 184 234 0.0 6 231 2 1 185 184 0.0 6 182 1 1 186 262 0.0 6 262 187 206 0.0 6 206 188 252 0.0 6 252 189 288 0.0 6 286 2 190 437 0.0 6 436 1 191 306 0.0 6 305 1 192 274 0.0 6 272 2 193 299 0.0 6 296 3 194 441 0.0 6 436 5 195 437 0.0 6 436 1 196 412 0.0 6 408 4 197 552 0.0 6 548 3 1 198 343 0.0 6 342 1 199 292 0.0 6 291 1 200 355 0.0 6 353 2 201 288 0.0 6 284 4 202 391 0.0 6 391 203 485 0.0 6 482 3 204 389 0.0 6 387 2 205 491 0.0 6 489 2 206 445 0.0 6 442 3 207 436 0.0 6 429 5 1 0 0 0 1 208 366 0.0 6 363 3 209 388 0.0 6 388 210 560 0.0 6 558 2 211 445 0.0 6 439 4 1 0 1 212 476 0.0 6 473 3 213 590 0.0 6 587 3 214 658 0.0 6 657 1 215 514 0.0 6 511 1 1 0 0 1 216 470 0.0 6 467 2 0 0 0 0 1 217 537 0.0 6 536 0 0 1 218 506 0.0 6 503 3 219 422 0.0 6 420 2 220 394 0.0 6 391 2 0 0 1 221 482 0.0 6 481 1 222 463 0.0 6 460 3 223 468 0.0 6 464 4 224 537 0.0 6 534 3 225 510 0.0 6 510 226 378 0.0 6 376 2 227 344 0.0 6 343 1 228 366 0.0 6 363 3 229 401 0.0 6 398 3 230 320 0.0 6 320 231 369 0.0 6 366 3 232 342 0.0 6 339 3 233 376 0.0 6 375 1 234 288 0.0 6 288 235 304 0.0 6 304 236 369 0.0 6 369 237 336 0.0 6 336 238 316 0.0 6 313 3 239 366 0.0 6 361 4 0 0 0 1 240 330 0.0 6 328 2 241 283 0.0 6 282 1 242 301 0.0 6 298 3 243 247 0.0 6 246 1 244 239 0.0 6 236 2 0 0 0 1 245 240 0.0 6 240 246 247 0.0 6 246 1 247 204 0.0 6 203 0 0 0 0 0 1 248 205 0.0 6 205 249 206 0.0 6 205 1 250 210 0.0 6 209 1 251 162 0.0 6 162 252 185 0.0 6 185 253 159 0.0 6 157 2 254 181 0.0 6 181 255 159 0.0 6 158 0 0 0 1 256 194 0.0 6 194 257 141 0.0 6 140 1 258 189 0.0 6 189 259 137 0.0 6 137 260 110 0.0 6 109 1 261 115 0.0 6 114 1 262 126 0.0 6 126 263 116 0.0 6 116 264 111 0.0 6 109 1 0 0 0 1 265 100 0.0 6 100 266 117 0.0 6 116 1 267 107 0.0 6 107 268 134 0.0 6 134 269 126 0.0 6 125 1 270 101 0.0 6 101 271 101 0.0 6 100 1 272 128 0.0 6 126 1 0 0 0 1 273 113 0.0 6 112 1 274 149 0.0 6 147 2 275 160 0.0 6 157 3 276 211 0.0 6 211 277 767 0.0 6 760 6 0 0 0 1 278 835 0.0 6 833 2 279 18 0.0 6 17 1 280 1 0.0 6 1 281 1 0.0 6 1 289 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1077 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 2 0.0 6 1 1 64 89 0.0 6 87 2 73 4 0.0 6 4 74 9 0.0 6 9 75 12 0.0 6 12 76 6 0.0 6 6 77 7 0.0 6 7 78 8 0.0 6 8 79 7 0.0 6 7 80 12 0.0 6 12 81 7 0.0 6 7 82 17 0.0 6 17 83 15 0.0 6 15 84 6 0.0 6 6 85 8 0.0 6 8 86 7 0.0 6 7 87 4 0.0 6 4 88 3 0.0 6 3 89 5 0.0 6 5 90 6 0.0 6 6 91 5 0.0 6 5 92 7 0.0 6 7 93 6 0.0 6 6 94 9 0.0 6 9 95 3 0.0 6 3 96 6 0.0 6 6 97 7 0.0 6 7 98 9 0.0 6 9 99 4 0.0 6 4 100 3 0.0 6 3 101 5 0.0 6 5 102 5 0.0 6 5 103 6 0.0 6 6 104 5 0.0 6 5 105 6 0.0 6 6 106 2 0.0 6 2 107 2 0.0 6 2 108 4 0.0 6 4 109 2 0.0 6 2 110 10 0.0 6 10 111 16 0.0 6 16 112 10 0.0 6 10 113 1 0.0 6 1 114 7 0.0 6 7 115 5 0.0 6 5 116 1 0.0 6 1 117 3 0.0 6 3 118 4 0.0 6 4 119 8 0.0 6 8 120 5 0.0 6 5 121 5 0.0 6 5 122 6 0.0 6 6 124 3 0.0 6 3 125 3 0.0 6 3 126 5 0.0 6 5 127 10 0.0 6 10 128 6 0.0 6 6 129 3 0.0 6 3 130 2 0.0 6 2 131 1 0.0 6 1 132 1 0.0 6 1 133 3 0.0 6 3 134 9 0.0 6 9 135 8 0.0 6 8 136 6 0.0 6 6 137 2 0.0 6 2 138 3 0.0 6 3 139 1 0.0 6 1 140 4 0.0 6 4 141 4 0.0 6 4 142 9 0.0 6 9 143 3 0.0 6 3 144 1 0.0 6 0 0 0 0 0 0 1 145 9 0.0 6 9 146 7 0.0 6 7 147 6 0.0 6 6 148 5 0.0 6 5 149 3 0.0 6 3 150 2 0.0 6 2 151 5 0.0 6 5 152 2 0.0 6 2 153 10 0.0 6 10 154 13 0.0 6 13 155 4 0.0 6 4 156 7 0.0 6 7 157 6 0.0 6 6 158 3 0.0 6 3 159 1 0.0 6 1 160 3 0.0 6 3 161 5 0.0 6 5 162 6 0.0 6 6 163 6 0.0 6 6 164 3 0.0 6 3 165 11 0.0 6 11 166 5 0.0 6 5 167 3 0.0 6 3 168 10 0.0 6 10 169 6 0.0 6 6 170 9 0.0 6 9 171 6 0.0 6 6 172 4 0.0 6 4 173 8 0.0 6 8 174 9 0.0 6 9 175 7 0.0 6 7 176 9 0.0 6 9 177 5 0.0 6 5 178 7 0.0 6 7 179 9 0.0 6 9 180 1 0.0 6 1 181 5 0.0 6 5 182 10 0.0 6 10 183 15 0.0 6 15 184 11 0.0 6 11 185 8 0.0 6 8 186 7 0.0 6 7 187 4 0.0 6 4 188 1 0.0 6 1 189 15 0.0 6 15 190 17 0.0 6 17 191 6 0.0 6 6 192 4 0.0 6 4 193 7 0.0 6 7 194 9 0.0 6 9 195 9 0.0 6 9 196 5 0.0 6 4 0 0 0 0 0 1 197 1 0.0 6 1 198 7 0.0 6 7 199 6 0.0 6 6 200 2 0.0 6 2 201 2 0.0 6 2 202 3 0.0 6 3 203 2 0.0 6 2 204 9 0.0 6 9 205 8 0.0 6 8 206 5 0.0 6 5 207 8 0.0 6 8 208 3 0.0 6 3 209 12 0.0 6 12 210 2 0.0 6 2 211 2 0.0 6 2 212 6 0.0 6 6 213 5 0.0 6 5 214 4 0.0 6 4 215 4 0.0 6 4 216 5 0.0 6 5 217 2 0.0 6 2 218 2 0.0 6 2 219 4 0.0 6 4 220 2 0.0 6 2 221 3 0.0 6 3 222 6 0.0 6 6 223 4 0.0 6 4 224 3 0.0 6 3 225 1 0.0 6 1 226 4 0.0 6 4 227 3 0.0 6 3 228 5 0.0 6 5 229 2 0.0 6 2 230 3 0.0 6 3 231 4 0.0 6 4 232 6 0.0 6 6 233 2 0.0 6 2 235 2 0.0 6 2 236 1 0.0 6 1 237 4 0.0 6 4 238 5 0.0 6 5 240 5 0.0 6 5 242 2 0.0 6 2 245 1 0.0 6 1 246 2 0.0 6 2 247 1 0.0 6 1 248 3 0.0 6 3 249 5 0.0 6 5 250 4 0.0 6 4 251 1 0.0 6 1 252 2 0.0 6 2 253 4 0.0 6 4 254 1 0.0 6 1 255 1 0.0 6 1 258 1 0.0 6 1 259 1 0.0 6 1 260 5 0.0 6 5 261 1 0.0 6 1 262 1 0.0 6 1 266 4 0.0 6 4 268 1 0.0 6 1 270 2 0.0 6 2 271 1 0.0 6 1 273 5 0.0 6 5 275 2 0.0 6 2 276 1 0.0 6 1 278 1 0.0 6 1 279 3 0.0 6 3 282 1 0.0 6 1 284 2 0.0 6 2 289 1 0.0 6 1 294 1 0.0 6 1 296 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 42642 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 12 2 0.0 3 2 13 7 0.0 3 7 14 6 0.0 4 4 2 15 2 0.0 4 2 16 9 0.0 4 7 2 17 10 0.0 5 9 1 18 5 0.0 5 5 19 12 0.0 5 11 1 20 41 0.0 6 39 2 21 19 0.0 6 18 1 22 14 0.0 6 13 1 23 17 0.0 6 16 1 24 12 0.0 6 11 0 0 1 25 4 0.0 6 4 26 16 0.0 6 15 1 27 15 0.0 6 14 1 28 11 0.0 6 9 2 29 6 0.0 6 5 0 0 0 1 30 4 0.0 6 3 1 31 8 0.0 6 8 32 12 0.0 6 12 33 7 0.0 6 7 34 9 0.0 6 9 35 6 0.0 6 6 36 4 0.0 6 4 37 6 0.0 6 6 38 5 0.0 6 4 1 39 9 0.0 6 8 1 40 8 0.0 6 8 41 7 0.0 6 7 42 16 0.0 6 14 1 0 1 43 6 0.0 6 6 44 8 0.0 6 7 1 45 9 0.0 6 9 46 9 0.0 6 9 47 12 0.0 6 11 1 48 15 0.0 6 13 2 49 8 0.0 6 8 50 14 0.0 6 14 51 17 0.0 6 15 1 1 52 5 0.0 6 5 53 15 0.0 6 15 54 14 0.0 6 14 55 11 0.0 6 11 56 26 0.0 6 20 4 2 57 8 0.0 6 7 1 58 5 0.0 6 5 59 19 0.0 6 18 1 60 11 0.0 6 9 2 61 16 0.0 6 13 2 1 62 15 0.0 6 15 63 12 0.0 6 12 64 25 0.0 6 24 1 65 16 0.0 6 16 66 26 0.0 6 26 67 21 0.0 6 20 1 68 37 0.0 6 33 4 69 98 0.0 6 94 2 1 0 1 70 43 0.0 6 40 3 71 46 0.0 6 46 72 29 0.0 6 27 2 73 37 0.0 6 35 1 0 1 74 17 0.0 6 17 75 20 0.0 6 19 0 0 0 0 1 76 28 0.0 6 22 5 0 0 0 0 1 77 25 0.0 6 23 1 0 0 0 1 78 26 0.0 6 26 79 31 0.0 6 27 4 80 22 0.0 6 21 0 0 0 0 1 81 26 0.0 6 23 3 82 33 0.0 6 33 83 31 0.0 6 28 3 84 34 0.0 6 33 1 85 41 0.0 6 40 0 0 1 86 37 0.0 6 32 5 87 31 0.0 6 28 3 88 26 0.0 6 24 2 89 38 0.0 6 37 0 0 0 1 90 34 0.0 6 31 2 1 91 37 0.0 6 35 1 0 0 0 1 92 50 0.0 6 47 3 93 47 0.0 6 46 1 94 37 0.0 6 36 1 95 48 0.0 6 48 96 35 0.0 6 33 2 97 49 0.0 6 46 1 0 0 1 1 98 57 0.0 6 56 1 99 37 0.0 6 36 1 100 48 0.0 6 46 2 101 51 0.0 6 51 102 59 0.0 6 57 1 0 0 1 103 62 0.0 6 59 3 104 67 0.0 6 67 105 64 0.0 6 63 1 106 134 0.0 6 134 107 71 0.0 6 69 1 0 1 108 52 0.0 6 49 3 109 73 0.0 6 69 4 110 78 0.0 6 75 3 111 80 0.0 6 80 112 85 0.0 6 83 2 113 96 0.0 6 92 3 0 0 0 0 1 114 107 0.0 6 106 1 115 80 0.0 6 79 1 116 84 0.0 6 82 1 0 1 117 114 0.0 6 112 1 1 118 94 0.0 6 91 2 1 119 137 0.0 6 128 8 0 1 120 103 0.0 6 101 2 121 117 0.0 6 115 2 122 116 0.0 6 114 2 123 146 0.0 6 144 1 0 0 1 124 131 0.0 6 125 6 125 147 0.0 6 144 3 126 153 0.0 6 151 1 0 0 0 1 127 190 0.0 6 187 3 128 206 0.0 6 201 4 0 1 129 204 0.0 6 198 5 0 0 0 1 130 208 0.0 6 202 5 0 0 0 0 1 131 253 0.0 6 247 5 1 132 288 0.0 6 282 5 0 1 133 271 0.0 6 267 4 134 292 0.0 6 288 4 135 288 0.0 6 282 4 0 0 0 1 1 136 324 0.0 6 320 3 0 1 137 336 0.0 6 328 7 1 138 264 0.0 6 258 3 0 0 2 0 1 139 341 0.0 6 336 3 0 1 1 140 346 0.0 6 339 4 1 1 0 0 1 141 463 0.0 6 456 6 0 0 0 1 142 538 0.0 6 531 6 1 143 625 0.0 6 620 3 1 0 0 1 144 596 0.0 6 589 5 0 0 1 1 145 579 0.0 6 574 4 0 1 146 793 0.0 6 785 6 0 0 1 1 147 629 0.0 6 622 6 1 148 820 0.0 6 811 8 0 0 0 1 149 730 0.0 6 723 7 150 840 0.0 6 827 11 0 1 0 0 1 151 1014 0.0 6 1004 10 152 1093 0.0 6 1081 10 0 0 1 1 153 784 0.0 6 780 3 0 1 154 901 0.0 6 891 8 1 0 1 155 919 0.0 6 908 11 156 773 0.0 6 757 11 3 0 1 1 157 815 0.0 6 807 8 158 729 0.0 6 718 9 0 1 0 1 159 738 0.0 6 731 4 1 2 160 770 0.0 6 764 5 0 0 0 1 161 692 0.0 6 682 9 1 162 647 0.0 6 642 4 0 1 163 662 0.0 6 655 7 164 579 0.0 6 571 4 0 1 2 1 165 648 0.0 6 643 4 1 166 586 0.0 6 585 1 167 566 0.0 6 557 9 168 718 0.0 6 712 4 0 2 169 521 0.0 6 510 11 170 652 0.0 6 650 1 1 171 595 0.0 6 585 9 1 172 510 0.0 6 504 5 1 173 612 0.0 6 605 6 1 174 599 0.0 6 592 5 1 0 0 1 175 527 0.0 6 523 4 176 528 0.0 6 520 7 0 1 177 541 0.0 6 536 5 178 562 0.0 6 557 3 1 0 1 179 450 0.0 6 446 2 1 0 0 0 1 180 440 0.0 6 437 3 181 346 0.0 6 345 1 182 350 0.0 6 346 2 0 0 1 1 183 323 0.0 6 320 3 184 307 0.0 6 307 185 486 0.0 6 479 7 186 559 0.0 6 555 3 0 0 0 0 1 187 468 0.0 6 462 5 0 0 0 0 1 188 387 0.0 6 384 3 189 346 0.0 6 345 1 190 306 0.0 6 306 191 268 0.0 6 267 1 192 282 0.0 6 280 1 0 0 0 0 1 193 268 0.0 6 267 1 194 182 0.0 6 182 195 171 0.0 6 167 3 1 196 155 0.0 6 151 4 197 167 0.0 6 161 3 2 0 0 1 198 246 0.0 6 242 3 0 0 0 1 199 185 0.0 6 181 4 200 131 0.0 6 130 1 201 130 0.0 6 129 1 202 125 0.0 6 122 2 1 203 126 0.0 6 126 204 114 0.0 6 114 205 106 0.0 6 106 206 95 0.0 6 94 1 207 80 0.0 6 78 2 208 74 0.0 6 73 1 209 61 0.0 6 61 210 61 0.0 6 61 211 61 0.0 6 61 212 44 0.0 6 43 1 213 52 0.0 6 51 1 214 62 0.0 6 59 3 215 49 0.0 6 49 216 54 0.0 6 51 3 217 42 0.0 6 41 0 0 0 0 1 218 32 0.0 6 32 219 28 0.0 6 28 220 42 0.0 6 42 221 26 0.0 6 26 222 23 0.0 6 23 223 26 0.0 6 25 1 224 31 0.0 6 31 225 21 0.0 6 20 1 226 14 0.0 6 14 227 8 0.0 6 8 228 19 0.0 6 19 229 10 0.0 6 10 230 17 0.0 6 17 231 12 0.0 6 12 232 3 0.0 6 3 233 8 0.0 6 8 235 4 0.0 6 4 237 3 0.0 6 2 1 238 1 0.0 6 1 239 2 0.0 6 2 240 2 0.0 6 2 241 2 0.0 6 2 242 2 0.0 6 2 243 6 0.0 6 5 1 244 1 0.0 6 1 247 1 0.0 6 1 248 3 0.0 6 3 249 1 0.0 6 1 250 2 0.0 6 2 251 1 0.0 6 1 252 1 0.0 6 1 279 1 0.0 6 1 280 4 0.0 6 4 281 33 0.0 6 33 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 894 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 2 0.0 6 2 70 12 0.0 6 12 71 10 0.0 6 10 72 12 0.0 6 11 1 73 8 0.0 6 8 74 10 0.0 6 10 75 14 0.0 6 14 76 8 0.0 6 8 77 19 0.0 6 19 78 18 0.0 6 17 1 79 12 0.0 6 12 80 20 0.0 6 20 81 9 0.0 6 9 82 9 0.0 6 9 83 3 0.0 6 2 0 0 0 1 84 8 0.0 6 8 85 3 0.0 6 3 86 5 0.0 6 5 87 10 0.0 6 10 88 6 0.0 6 6 89 8 0.0 6 8 90 10 0.0 6 10 91 8 0.0 6 8 92 6 0.0 6 6 93 9 0.0 6 9 94 3 0.0 6 3 95 13 0.0 6 13 96 10 0.0 6 10 97 4 0.0 6 4 98 8 0.0 6 8 99 5 0.0 6 4 0 0 1 100 6 0.0 6 6 101 4 0.0 6 4 102 5 0.0 6 4 1 103 2 0.0 6 2 104 8 0.0 6 8 105 5 0.0 6 5 106 3 0.0 6 3 107 8 0.0 6 8 108 10 0.0 6 10 109 13 0.0 6 13 110 3 0.0 6 3 111 6 0.0 6 6 112 5 0.0 6 5 113 3 0.0 6 3 114 3 0.0 6 3 115 7 0.0 6 7 116 5 0.0 6 5 117 7 0.0 6 7 118 1 0.0 6 1 119 10 0.0 6 10 120 6 0.0 6 6 121 3 0.0 6 3 122 4 0.0 6 4 123 6 0.0 6 6 124 12 0.0 6 12 125 11 0.0 6 11 126 6 0.0 6 6 127 1 0.0 6 1 128 3 0.0 6 3 129 3 0.0 6 3 130 3 0.0 6 3 131 7 0.0 6 7 132 12 0.0 6 12 133 5 0.0 6 5 134 1 0.0 6 1 135 9 0.0 6 9 136 9 0.0 6 9 137 7 0.0 6 7 138 9 0.0 6 8 0 0 0 1 139 10 0.0 6 10 140 5 0.0 6 5 141 5 0.0 6 5 142 7 0.0 6 7 143 6 0.0 6 6 144 4 0.0 6 4 145 3 0.0 6 3 146 5 0.0 6 5 147 5 0.0 6 5 148 3 0.0 6 3 149 8 0.0 6 8 150 7 0.0 6 7 151 3 0.0 6 3 152 7 0.0 6 7 153 7 0.0 6 7 154 7 0.0 6 7 155 5 0.0 6 5 156 1 0.0 6 1 157 8 0.0 6 8 158 10 0.0 6 10 159 4 0.0 6 4 160 5 0.0 6 5 161 12 0.0 6 12 162 13 0.0 6 13 163 4 0.0 6 4 164 21 0.0 6 21 165 5 0.0 6 5 166 6 0.0 6 6 167 6 0.0 6 6 168 6 0.0 6 6 169 6 0.0 6 6 170 2 0.0 6 2 171 3 0.0 6 3 172 12 0.0 6 12 173 6 0.0 6 6 174 9 0.0 6 9 175 7 0.0 6 7 176 7 0.0 6 7 177 7 0.0 6 7 178 3 0.0 6 3 179 4 0.0 6 4 180 5 0.0 6 5 181 6 0.0 6 6 182 4 0.0 6 3 0 0 0 1 183 15 0.0 6 15 184 5 0.0 6 5 185 5 0.0 6 5 186 7 0.0 6 7 187 19 0.0 6 19 188 3 0.0 6 3 189 2 0.0 6 2 190 4 0.0 6 4 191 7 0.0 6 7 192 1 0.0 6 1 193 1 0.0 6 1 194 3 0.0 6 3 195 2 0.0 6 2 196 3 0.0 6 3 197 1 0.0 6 1 199 2 0.0 6 2 200 1 0.0 6 0 0 0 0 1 201 1 0.0 6 1 202 1 0.0 6 1 203 1 0.0 6 1 204 1 0.0 6 1 205 2 0.0 6 2 206 2 0.0 6 2 208 4 0.0 6 4 209 1 0.0 6 1 210 1 0.0 6 1 211 1 0.0 6 1 219 2 0.0 6 2 222 1 0.0 6 1 223 1 0.0 6 1 226 1 0.0 6 1 247 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_07_151_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_07_378_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_07_151_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_07_378_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.21 s (81 µs/read; 0.74 M reads/minute). === Summary === Total read pairs processed: 51,759 Read 1 with adapter: 51,752 (100.0%) Read 2 with adapter: 51,723 (99.9%) == Read fate breakdown == Pairs that were too short: 20 (0.0%) Pairs discarded as untrimmed: 43 (0.1%) Pairs written (passing filters): 51,696 (99.9%) Total basepairs processed: 21,786,615 bp Read 1: 12,161,797 bp Read 2: 9,624,818 bp Total written (filtered): 3,393,318 bp (15.6%) Read 1: 1,710,040 bp Read 2: 1,683,278 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 50586 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 808.7 0 0 2 4 5 202.2 1 4 1 5 1 50.5 1 1 7 2 3.2 2 1 0 1 10 2 0.0 3 2 13 2 0.0 3 2 15 1 0.0 4 1 16 1 0.0 4 1 17 6 0.0 5 5 1 18 2 0.0 5 1 1 20 75 0.0 6 72 3 21 40 0.0 6 36 4 22 33 0.0 6 33 23 36 0.0 6 36 24 15 0.0 6 15 25 16 0.0 6 15 1 26 16 0.0 6 15 0 1 27 7 0.0 6 7 28 9 0.0 6 8 1 29 8 0.0 6 8 30 7 0.0 6 7 31 7 0.0 6 7 32 7 0.0 6 5 2 33 7 0.0 6 7 34 10 0.0 6 8 1 1 35 8 0.0 6 7 1 36 8 0.0 6 7 1 37 7 0.0 6 6 1 38 5 0.0 6 4 1 39 12 0.0 6 11 1 40 11 0.0 6 10 1 41 8 0.0 6 7 1 42 5 0.0 6 4 1 43 8 0.0 6 8 44 10 0.0 6 9 1 45 4 0.0 6 4 46 8 0.0 6 8 47 9 0.0 6 7 0 0 0 0 1 1 48 9 0.0 6 9 49 12 0.0 6 11 1 50 4 0.0 6 4 51 9 0.0 6 8 0 0 1 52 3 0.0 6 3 53 7 0.0 6 6 0 1 54 5 0.0 6 5 55 5 0.0 6 4 1 56 5 0.0 6 5 57 5 0.0 6 4 1 58 8 0.0 6 7 1 59 5 0.0 6 5 60 3 0.0 6 3 61 5 0.0 6 5 62 6 0.0 6 6 63 5 0.0 6 4 1 64 7 0.0 6 5 2 65 9 0.0 6 9 66 6 0.0 6 6 67 9 0.0 6 9 68 4 0.0 6 4 69 9 0.0 6 9 70 10 0.0 6 9 1 71 4 0.0 6 3 1 72 9 0.0 6 9 73 11 0.0 6 10 1 74 6 0.0 6 6 75 6 0.0 6 5 1 76 8 0.0 6 8 77 6 0.0 6 6 78 8 0.0 6 8 79 5 0.0 6 5 80 6 0.0 6 6 81 7 0.0 6 7 82 10 0.0 6 10 83 16 0.0 6 15 0 0 0 0 0 1 84 8 0.0 6 8 85 12 0.0 6 11 1 86 6 0.0 6 6 87 6 0.0 6 5 1 88 9 0.0 6 9 89 11 0.0 6 10 1 90 6 0.0 6 6 91 4 0.0 6 4 92 8 0.0 6 8 93 5 0.0 6 5 94 6 0.0 6 6 95 9 0.0 6 9 96 9 0.0 6 9 97 6 0.0 6 6 98 9 0.0 6 7 2 99 7 0.0 6 7 100 14 0.0 6 12 2 101 11 0.0 6 10 1 102 19 0.0 6 19 103 7 0.0 6 7 104 16 0.0 6 16 105 19 0.0 6 19 106 15 0.0 6 15 107 8 0.0 6 8 108 10 0.0 6 10 109 13 0.0 6 13 110 14 0.0 6 13 0 0 0 1 111 14 0.0 6 13 1 112 17 0.0 6 16 1 113 22 0.0 6 21 1 114 13 0.0 6 12 0 0 0 0 1 115 18 0.0 6 17 0 0 0 0 0 1 116 21 0.0 6 21 117 18 0.0 6 18 118 19 0.0 6 19 119 19 0.0 6 19 120 17 0.0 6 16 0 0 0 0 1 121 24 0.0 6 22 2 122 15 0.0 6 13 2 123 28 0.0 6 28 124 16 0.0 6 16 125 24 0.0 6 23 0 1 126 35 0.0 6 34 1 127 22 0.0 6 19 1 0 2 128 23 0.0 6 23 129 21 0.0 6 20 1 130 27 0.0 6 26 1 131 32 0.0 6 31 1 132 26 0.0 6 25 1 133 34 0.0 6 32 2 134 32 0.0 6 32 135 31 0.0 6 30 1 136 32 0.0 6 32 137 38 0.0 6 38 138 39 0.0 6 39 139 40 0.0 6 40 140 31 0.0 6 31 141 59 0.0 6 57 1 0 1 142 74 0.0 6 74 143 146 0.0 6 146 144 338 0.0 6 337 1 145 448 0.0 6 447 0 0 1 146 311 0.0 6 311 147 351 0.0 6 351 148 454 0.0 6 452 2 149 380 0.0 6 380 150 318 0.0 6 318 151 434 0.0 6 433 1 152 410 0.0 6 410 153 354 0.0 6 354 154 275 0.0 6 272 2 0 1 155 237 0.0 6 235 1 0 0 0 0 1 156 255 0.0 6 255 157 286 0.0 6 286 158 282 0.0 6 282 159 267 0.0 6 266 1 160 334 0.0 6 333 1 161 278 0.0 6 275 3 162 309 0.0 6 309 163 293 0.0 6 293 164 261 0.0 6 260 1 165 316 0.0 6 316 166 337 0.0 6 331 5 0 0 0 0 1 167 268 0.0 6 267 1 168 254 0.0 6 252 1 1 169 272 0.0 6 269 3 170 253 0.0 6 253 171 300 0.0 6 299 1 172 303 0.0 6 300 2 0 0 0 1 173 301 0.0 6 298 3 174 307 0.0 6 307 175 318 0.0 6 317 1 176 284 0.0 6 283 1 177 207 0.0 6 204 1 0 0 1 0 1 178 283 0.0 6 283 179 259 0.0 6 255 4 180 340 0.0 6 339 1 181 823 0.0 6 821 2 182 375 0.0 6 374 1 183 342 0.0 6 340 2 184 337 0.0 6 335 2 185 289 0.0 6 286 3 186 287 0.0 6 287 187 287 0.0 6 286 1 188 295 0.0 6 293 1 1 189 290 0.0 6 290 190 316 0.0 6 313 2 1 191 305 0.0 6 303 2 192 370 0.0 6 368 1 1 193 334 0.0 6 332 2 194 393 0.0 6 392 0 1 195 530 0.0 6 522 8 196 609 0.0 6 604 5 197 548 0.0 6 544 2 0 0 1 0 1 198 674 0.0 6 672 2 199 592 0.0 6 590 1 1 200 441 0.0 6 438 3 201 462 0.0 6 458 4 202 501 0.0 6 499 1 0 1 203 463 0.0 6 463 204 538 0.0 6 531 2 0 2 2 1 205 624 0.0 6 621 3 206 594 0.0 6 590 3 0 0 1 207 624 0.0 6 620 4 208 605 0.0 6 603 2 209 415 0.0 6 414 1 210 509 0.0 6 507 1 0 0 0 1 211 567 0.0 6 565 2 212 625 0.0 6 624 1 213 613 0.0 6 608 5 214 673 0.0 6 673 215 762 0.0 6 758 4 216 627 0.0 6 621 6 217 649 0.0 6 646 2 0 1 218 626 0.0 6 622 4 219 540 0.0 6 538 2 220 516 0.0 6 511 4 0 0 1 221 484 0.0 6 479 4 0 0 0 0 1 222 492 0.0 6 490 1 0 0 0 1 223 545 0.0 6 541 3 1 224 516 0.0 6 515 0 0 1 225 615 0.0 6 613 2 226 598 0.0 6 595 3 227 476 0.0 6 474 1 1 228 447 0.0 6 443 4 229 418 0.0 6 417 0 1 230 443 0.0 6 443 231 474 0.0 6 472 2 232 441 0.0 6 441 233 435 0.0 6 429 4 0 0 2 234 461 0.0 6 457 4 235 362 0.0 6 360 2 236 364 0.0 6 361 3 237 395 0.0 6 394 1 238 390 0.0 6 390 239 376 0.0 6 371 4 0 0 1 240 347 0.0 6 346 1 241 369 0.0 6 367 2 242 337 0.0 6 336 1 243 301 0.0 6 300 0 1 244 319 0.0 6 319 245 283 0.0 6 283 246 223 0.0 6 220 2 0 1 247 226 0.0 6 223 2 0 0 0 1 248 226 0.0 6 225 1 249 192 0.0 6 192 250 202 0.0 6 201 1 251 202 0.0 6 201 0 0 1 252 196 0.0 6 195 1 253 201 0.0 6 200 0 1 254 192 0.0 6 190 2 255 169 0.0 6 168 1 256 186 0.0 6 186 257 143 0.0 6 143 258 167 0.0 6 167 259 189 0.0 6 189 260 160 0.0 6 159 1 261 113 0.0 6 113 262 135 0.0 6 135 263 123 0.0 6 123 264 110 0.0 6 110 265 105 0.0 6 104 1 266 102 0.0 6 102 267 89 0.0 6 88 1 268 103 0.0 6 103 269 114 0.0 6 114 270 80 0.0 6 80 271 72 0.0 6 72 272 86 0.0 6 86 273 101 0.0 6 101 274 106 0.0 6 106 275 129 0.0 6 129 276 131 0.0 6 130 1 277 214 0.0 6 214 278 650 0.0 6 647 3 279 1042 0.0 6 1037 4 0 0 0 0 1 280 5 0.0 6 5 281 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1166 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 52 3 0.0 6 3 62 6 0.0 6 0 6 63 33 0.0 6 30 3 68 1 0.0 6 1 70 2 0.0 6 2 72 3 0.0 6 3 73 9 0.0 6 9 74 14 0.0 6 14 75 12 0.0 6 12 76 18 0.0 6 18 77 16 0.0 6 16 78 5 0.0 6 5 79 12 0.0 6 12 80 11 0.0 6 11 81 12 0.0 6 12 82 9 0.0 6 9 83 4 0.0 6 4 84 7 0.0 6 7 85 6 0.0 6 6 86 11 0.0 6 11 87 6 0.0 6 5 1 88 9 0.0 6 9 89 5 0.0 6 5 90 6 0.0 6 6 91 10 0.0 6 10 92 9 0.0 6 9 93 4 0.0 6 4 94 5 0.0 6 5 95 7 0.0 6 6 1 96 6 0.0 6 6 97 7 0.0 6 7 98 7 0.0 6 7 99 4 0.0 6 4 100 11 0.0 6 11 101 11 0.0 6 11 102 5 0.0 6 5 103 4 0.0 6 4 104 1 0.0 6 1 105 2 0.0 6 2 106 6 0.0 6 6 107 2 0.0 6 2 108 3 0.0 6 3 109 8 0.0 6 8 110 25 0.0 6 25 111 6 0.0 6 6 112 5 0.0 6 5 113 5 0.0 6 5 114 4 0.0 6 4 115 6 0.0 6 6 116 4 0.0 6 4 117 4 0.0 6 4 118 8 0.0 6 8 119 3 0.0 6 3 120 8 0.0 6 8 121 4 0.0 6 4 122 4 0.0 6 4 123 4 0.0 6 4 124 4 0.0 6 4 125 9 0.0 6 9 126 2 0.0 6 2 127 9 0.0 6 9 128 5 0.0 6 5 129 1 0.0 6 1 130 5 0.0 6 5 131 7 0.0 6 7 132 4 0.0 6 4 133 8 0.0 6 8 134 10 0.0 6 10 135 6 0.0 6 6 136 2 0.0 6 2 137 5 0.0 6 5 138 4 0.0 6 4 139 2 0.0 6 2 140 2 0.0 6 2 141 1 0.0 6 1 142 7 0.0 6 7 143 4 0.0 6 4 144 5 0.0 6 5 145 7 0.0 6 7 146 10 0.0 6 10 147 4 0.0 6 4 148 8 0.0 6 8 149 5 0.0 6 5 150 9 0.0 6 9 151 3 0.0 6 3 152 1 0.0 6 1 153 5 0.0 6 5 154 7 0.0 6 7 155 4 0.0 6 4 156 5 0.0 6 5 157 8 0.0 6 8 158 6 0.0 6 6 159 9 0.0 6 9 160 3 0.0 6 3 161 3 0.0 6 3 162 11 0.0 6 11 163 9 0.0 6 9 164 10 0.0 6 10 165 9 0.0 6 9 166 10 0.0 6 10 167 5 0.0 6 5 168 4 0.0 6 4 169 7 0.0 6 7 170 7 0.0 6 7 171 2 0.0 6 2 172 10 0.0 6 10 173 13 0.0 6 13 174 8 0.0 6 8 175 6 0.0 6 6 176 10 0.0 6 9 1 177 4 0.0 6 4 178 4 0.0 6 4 179 12 0.0 6 12 180 8 0.0 6 8 181 9 0.0 6 9 182 7 0.0 6 7 183 8 0.0 6 8 184 7 0.0 6 7 185 10 0.0 6 10 186 8 0.0 6 8 187 7 0.0 6 7 188 7 0.0 6 7 189 7 0.0 6 7 190 6 0.0 6 6 191 4 0.0 6 4 192 6 0.0 6 6 193 8 0.0 6 8 194 8 0.0 6 8 195 5 0.0 6 5 196 5 0.0 6 5 197 4 0.0 6 4 198 12 0.0 6 12 199 5 0.0 6 5 200 10 0.0 6 10 201 2 0.0 6 2 202 11 0.0 6 11 203 7 0.0 6 7 204 4 0.0 6 4 205 5 0.0 6 4 1 206 6 0.0 6 6 207 5 0.0 6 5 208 5 0.0 6 5 209 3 0.0 6 3 210 2 0.0 6 2 211 4 0.0 6 4 212 10 0.0 6 10 213 2 0.0 6 2 214 5 0.0 6 5 215 6 0.0 6 6 216 5 0.0 6 5 217 5 0.0 6 5 218 5 0.0 6 5 219 3 0.0 6 3 220 6 0.0 6 6 221 7 0.0 6 7 222 2 0.0 6 2 223 7 0.0 6 6 1 224 3 0.0 6 3 225 7 0.0 6 7 226 2 0.0 6 2 227 7 0.0 6 7 228 8 0.0 6 8 229 5 0.0 6 5 230 8 0.0 6 8 231 8 0.0 6 8 232 2 0.0 6 2 233 4 0.0 6 4 234 1 0.0 6 1 235 5 0.0 6 4 0 0 0 0 0 1 236 5 0.0 6 5 237 4 0.0 6 4 238 3 0.0 6 3 239 2 0.0 6 2 241 1 0.0 6 1 242 3 0.0 6 3 243 4 0.0 6 4 244 2 0.0 6 2 245 2 0.0 6 2 246 2 0.0 6 2 247 2 0.0 6 2 248 3 0.0 6 3 249 1 0.0 6 1 250 2 0.0 6 2 251 2 0.0 6 2 252 4 0.0 6 4 253 2 0.0 6 2 254 1 0.0 6 1 255 1 0.0 6 1 257 1 0.0 6 1 258 1 0.0 6 1 259 1 0.0 6 1 260 4 0.0 6 4 261 1 0.0 6 1 262 2 0.0 6 2 263 2 0.0 6 2 264 2 0.0 6 2 266 1 0.0 6 1 268 3 0.0 6 3 269 1 0.0 6 1 271 1 0.0 6 1 272 2 0.0 6 2 273 1 0.0 6 1 274 1 0.0 6 1 275 1 0.0 6 1 276 1 0.0 6 1 277 1 0.0 6 1 279 1 0.0 6 1 286 1 0.0 6 1 297 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 50746 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 808.7 0 0 1 6 2 12.6 1 2 9 1 0.2 2 1 10 4 0.0 3 4 11 3 0.0 3 3 12 3 0.0 3 3 13 13 0.0 3 13 14 6 0.0 4 5 1 15 2 0.0 4 1 1 16 13 0.0 4 10 3 17 28 0.0 5 25 2 1 18 22 0.0 5 20 2 19 19 0.0 5 17 2 20 71 0.0 6 69 2 21 44 0.0 6 40 4 22 21 0.0 6 19 2 23 23 0.0 6 23 24 16 0.0 6 16 25 26 0.0 6 26 26 16 0.0 6 15 1 27 16 0.0 6 15 0 1 28 14 0.0 6 12 2 29 10 0.0 6 10 30 15 0.0 6 15 31 23 0.0 6 18 4 1 32 15 0.0 6 14 1 33 7 0.0 6 7 34 16 0.0 6 15 1 35 7 0.0 6 5 1 1 36 13 0.0 6 12 1 37 11 0.0 6 11 38 8 0.0 6 6 2 39 9 0.0 6 9 40 13 0.0 6 11 2 41 13 0.0 6 11 1 1 42 12 0.0 6 10 2 43 8 0.0 6 8 44 12 0.0 6 12 45 13 0.0 6 13 46 15 0.0 6 13 1 1 47 19 0.0 6 18 1 48 28 0.0 6 24 3 1 49 18 0.0 6 16 2 50 16 0.0 6 14 2 51 22 0.0 6 21 1 52 13 0.0 6 13 53 10 0.0 6 10 54 21 0.0 6 20 1 55 8 0.0 6 8 56 17 0.0 6 13 4 57 20 0.0 6 18 2 58 28 0.0 6 28 59 17 0.0 6 17 60 14 0.0 6 13 1 61 21 0.0 6 20 1 62 22 0.0 6 19 2 0 1 63 29 0.0 6 26 2 1 64 18 0.0 6 17 1 65 27 0.0 6 25 2 66 35 0.0 6 32 1 2 67 101 0.0 6 96 5 68 52 0.0 6 44 6 1 0 1 69 56 0.0 6 51 3 0 1 0 1 70 35 0.0 6 34 1 71 34 0.0 6 31 2 0 0 0 0 1 72 25 0.0 6 24 1 73 29 0.0 6 26 3 74 43 0.0 6 40 3 75 32 0.0 6 30 1 0 0 1 76 32 0.0 6 31 1 77 30 0.0 6 29 1 78 41 0.0 6 39 2 79 47 0.0 6 43 3 0 0 0 0 1 80 46 0.0 6 42 4 81 41 0.0 6 39 2 82 63 0.0 6 58 4 0 0 0 0 1 83 60 0.0 6 56 4 84 39 0.0 6 38 1 85 52 0.0 6 49 2 0 0 1 86 39 0.0 6 39 87 32 0.0 6 30 1 0 0 1 88 46 0.0 6 45 0 0 1 89 46 0.0 6 45 1 90 61 0.0 6 60 1 91 44 0.0 6 42 2 92 53 0.0 6 52 1 93 66 0.0 6 64 2 94 58 0.0 6 56 1 0 0 1 95 61 0.0 6 59 1 0 0 0 0 1 96 71 0.0 6 69 1 0 0 0 1 97 64 0.0 6 61 3 98 85 0.0 6 80 4 0 0 0 1 99 61 0.0 6 57 3 0 0 1 100 71 0.0 6 66 3 1 0 1 101 60 0.0 6 59 1 102 68 0.0 6 65 3 103 80 0.0 6 77 3 104 180 0.0 6 174 5 0 1 105 86 0.0 6 84 2 106 89 0.0 6 88 1 107 93 0.0 6 90 2 0 0 0 1 108 96 0.0 6 93 2 0 0 1 109 93 0.0 6 89 2 0 1 0 1 110 109 0.0 6 106 3 111 112 0.0 6 108 3 0 1 112 134 0.0 6 133 0 0 1 113 100 0.0 6 95 4 0 0 0 0 1 114 125 0.0 6 123 2 115 111 0.0 6 109 2 116 122 0.0 6 121 1 117 146 0.0 6 140 4 0 1 0 1 118 149 0.0 6 147 2 119 136 0.0 6 135 0 0 0 0 0 1 120 136 0.0 6 131 3 1 0 1 121 138 0.0 6 135 3 122 158 0.0 6 157 1 123 181 0.0 6 176 5 124 223 0.0 6 213 9 0 1 125 217 0.0 6 214 3 126 193 0.0 6 192 1 127 254 0.0 6 249 4 0 1 128 257 0.0 6 249 8 129 248 0.0 6 244 3 0 1 130 281 0.0 6 272 9 131 379 0.0 6 366 11 1 0 0 0 1 132 315 0.0 6 311 2 1 0 0 0 1 133 331 0.0 6 324 4 1 1 0 0 1 134 395 0.0 6 388 7 135 359 0.0 6 354 5 136 366 0.0 6 356 9 0 0 0 0 1 137 383 0.0 6 378 4 0 0 0 0 1 138 404 0.0 6 397 6 0 0 1 139 400 0.0 6 396 4 140 520 0.0 6 508 10 2 141 717 0.0 6 708 9 142 876 0.0 6 865 7 1 0 1 0 2 143 702 0.0 6 694 7 0 0 1 144 831 0.0 6 821 9 0 0 1 145 852 0.0 6 841 11 146 875 0.0 6 861 10 0 2 0 0 2 147 880 0.0 6 870 8 0 0 0 2 148 1092 0.0 6 1086 6 149 1030 0.0 6 1011 17 0 0 1 0 1 150 1298 0.0 6 1279 17 0 1 0 1 151 982 0.0 6 967 14 1 152 1166 0.0 6 1145 18 0 2 0 1 153 1114 0.0 6 1093 19 0 0 0 2 154 897 0.0 6 887 10 155 967 0.0 6 950 13 1 0 1 1 1 156 906 0.0 6 899 4 0 0 1 2 157 915 0.0 6 905 7 1 1 0 0 1 158 998 0.0 6 985 10 1 1 0 1 159 799 0.0 6 793 6 160 869 0.0 6 850 12 4 1 0 1 1 161 762 0.0 6 753 7 2 162 707 0.0 6 700 5 2 163 739 0.0 6 730 7 0 0 1 1 164 661 0.0 6 650 10 0 0 0 0 1 165 600 0.0 6 592 7 0 0 0 0 1 166 820 0.0 6 813 5 0 0 1 0 1 167 654 0.0 6 645 8 1 168 819 0.0 6 812 6 0 0 0 0 1 169 667 0.0 6 660 4 0 0 3 170 719 0.0 6 716 2 0 1 171 721 0.0 6 715 4 0 1 0 0 1 172 787 0.0 6 776 9 1 0 1 173 692 0.0 6 687 5 174 671 0.0 6 662 4 2 1 1 1 175 629 0.0 6 628 1 176 709 0.0 6 700 8 0 0 0 0 1 177 547 0.0 6 543 4 178 601 0.0 6 598 3 179 386 0.0 6 382 3 0 0 1 180 411 0.0 6 410 1 181 357 0.0 6 354 3 182 367 0.0 6 364 2 0 0 0 0 1 183 550 0.0 6 544 6 184 680 0.0 6 677 2 0 1 185 546 0.0 6 537 7 0 0 1 1 186 388 0.0 6 384 3 0 0 0 1 187 369 0.0 6 365 4 188 344 0.0 6 342 1 0 0 0 1 189 329 0.0 6 326 1 0 0 0 1 1 190 297 0.0 6 294 3 191 304 0.0 6 301 3 192 235 0.0 6 233 2 193 207 0.0 6 205 2 194 174 0.0 6 172 2 195 162 0.0 6 161 1 196 241 0.0 6 237 2 0 0 0 0 2 197 183 0.0 6 179 4 198 145 0.0 6 145 199 142 0.0 6 142 200 111 0.0 6 111 201 115 0.0 6 115 202 125 0.0 6 125 203 105 0.0 6 105 204 107 0.0 6 107 205 97 0.0 6 96 1 206 85 0.0 6 84 1 207 77 0.0 6 77 208 76 0.0 6 76 209 72 0.0 6 72 210 51 0.0 6 50 1 211 62 0.0 6 62 212 47 0.0 6 47 213 66 0.0 6 66 214 66 0.0 6 66 215 42 0.0 6 40 1 0 1 216 55 0.0 6 53 2 217 34 0.0 6 34 218 31 0.0 6 31 219 36 0.0 6 35 0 0 0 0 0 1 220 21 0.0 6 21 221 26 0.0 6 25 1 222 15 0.0 6 15 223 22 0.0 6 22 224 15 0.0 6 15 225 14 0.0 6 14 226 8 0.0 6 7 1 227 10 0.0 6 10 228 11 0.0 6 11 229 7 0.0 6 7 230 5 0.0 6 5 231 4 0.0 6 4 232 9 0.0 6 9 233 7 0.0 6 6 0 1 234 2 0.0 6 2 235 3 0.0 6 3 236 5 0.0 6 5 237 2 0.0 6 2 238 2 0.0 6 2 239 1 0.0 6 1 240 1 0.0 6 1 241 1 0.0 6 1 242 1 0.0 6 1 243 2 0.0 6 2 245 1 0.0 6 1 246 2 0.0 6 2 247 1 0.0 6 1 251 1 0.0 6 1 277 1 0.0 6 1 278 12 0.0 6 11 0 0 0 0 0 1 279 54 0.0 6 54 280 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 977 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 49 1 0.0 6 0 1 52 3 0.0 6 3 54 2 0.0 6 2 56 1 0.0 6 1 59 1 0.0 6 0 1 62 2 0.0 6 2 65 3 0.0 6 3 68 1 0.0 6 1 71 7 0.0 6 7 72 3 0.0 6 3 73 15 0.0 6 15 74 12 0.0 6 12 75 11 0.0 6 11 76 12 0.0 6 12 77 11 0.0 6 11 78 19 0.0 6 19 79 14 0.0 6 14 80 10 0.0 6 10 81 13 0.0 6 12 0 0 1 82 14 0.0 6 14 83 10 0.0 6 10 84 6 0.0 6 6 85 5 0.0 6 5 86 8 0.0 6 8 87 4 0.0 6 4 88 8 0.0 6 8 89 12 0.0 6 12 90 14 0.0 6 14 91 7 0.0 6 7 92 6 0.0 6 6 93 7 0.0 6 7 94 9 0.0 6 9 95 7 0.0 6 7 96 10 0.0 6 10 97 6 0.0 6 6 98 4 0.0 6 4 99 6 0.0 6 6 100 4 0.0 6 4 101 4 0.0 6 4 102 5 0.0 6 5 103 9 0.0 6 9 104 11 0.0 6 11 105 4 0.0 6 4 106 4 0.0 6 4 107 2 0.0 6 2 108 4 0.0 6 4 109 18 0.0 6 18 110 33 0.0 6 33 111 8 0.0 6 8 112 5 0.0 6 5 113 7 0.0 6 7 114 7 0.0 6 7 115 6 0.0 6 6 116 7 0.0 6 7 117 3 0.0 6 3 118 10 0.0 6 10 119 5 0.0 6 5 120 4 0.0 6 4 121 2 0.0 6 2 122 4 0.0 6 4 123 4 0.0 6 4 124 6 0.0 6 6 125 10 0.0 6 10 126 5 0.0 6 5 127 10 0.0 6 10 128 7 0.0 6 7 129 8 0.0 6 8 130 6 0.0 6 6 131 5 0.0 6 5 132 3 0.0 6 3 133 7 0.0 6 7 134 13 0.0 6 13 135 10 0.0 6 10 136 11 0.0 6 11 137 14 0.0 6 14 138 5 0.0 6 5 139 12 0.0 6 12 140 5 0.0 6 5 141 8 0.0 6 8 142 5 0.0 6 5 143 4 0.0 6 4 144 6 0.0 6 6 145 11 0.0 6 11 146 7 0.0 6 7 147 7 0.0 6 7 148 4 0.0 6 4 149 9 0.0 6 8 0 0 0 1 150 11 0.0 6 11 151 6 0.0 6 6 152 5 0.0 6 5 153 1 0.0 6 1 154 7 0.0 6 7 155 2 0.0 6 2 156 10 0.0 6 10 157 7 0.0 6 7 158 9 0.0 6 9 159 9 0.0 6 9 160 1 0.0 6 1 161 6 0.0 6 6 162 5 0.0 6 5 163 8 0.0 6 8 164 13 0.0 6 13 165 6 0.0 6 6 166 3 0.0 6 3 167 9 0.0 6 9 168 6 0.0 6 6 169 5 0.0 6 5 170 11 0.0 6 11 171 9 0.0 6 9 172 8 0.0 6 8 173 7 0.0 6 7 174 6 0.0 6 6 175 6 0.0 6 6 176 4 0.0 6 4 178 8 0.0 6 8 179 10 0.0 6 10 180 6 0.0 6 6 181 6 0.0 6 6 182 4 0.0 6 4 183 1 0.0 6 1 184 3 0.0 6 3 185 7 0.0 6 7 186 4 0.0 6 4 187 5 0.0 6 5 188 2 0.0 6 2 189 11 0.0 6 11 190 2 0.0 6 2 191 2 0.0 6 2 192 5 0.0 6 5 193 2 0.0 6 2 194 5 0.0 6 5 195 2 0.0 6 2 196 1 0.0 6 1 197 4 0.0 6 4 198 4 0.0 6 4 199 4 0.0 6 4 200 4 0.0 6 3 1 201 4 0.0 6 4 202 3 0.0 6 3 203 5 0.0 6 5 204 1 0.0 6 1 205 1 0.0 6 1 206 5 0.0 6 5 208 4 0.0 6 4 209 1 0.0 6 1 210 1 0.0 6 1 211 1 0.0 6 1 213 1 0.0 6 1 214 2 0.0 6 1 1 215 4 0.0 6 4 216 1 0.0 6 1 220 2 0.0 6 2 225 3 0.0 6 3 226 1 0.0 6 1 227 1 0.0 6 1 247 1 0.0 6 1 253 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_08_152_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_08_379_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_08_152_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_08_379_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.57 s (71 µs/read; 0.85 M reads/minute). === Summary === Total read pairs processed: 78,645 Read 1 with adapter: 78,632 (100.0%) Read 2 with adapter: 78,645 (100.0%) == Read fate breakdown == Pairs that were too short: 22 (0.0%) Pairs discarded as untrimmed: 13 (0.0%) Pairs written (passing filters): 78,610 (100.0%) Total basepairs processed: 36,010,972 bp Read 1: 20,125,293 bp Read 2: 15,885,679 bp Total written (filtered): 5,051,341 bp (14.0%) Read 1: 2,579,808 bp Read 2: 2,471,533 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 77330 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1228.8 0 2 4 3 307.2 1 2 1 6 2 19.2 1 0 0 2 7 3 4.8 2 2 0 1 9 2 0.3 2 2 13 1 0.0 3 0 1 14 2 0.0 4 2 15 1 0.0 4 1 17 2 0.0 5 1 1 19 3 0.0 5 2 1 20 71 0.0 6 70 1 21 35 0.0 6 34 1 22 34 0.0 6 34 23 24 0.0 6 23 1 24 15 0.0 6 14 1 25 11 0.0 6 11 26 13 0.0 6 13 27 13 0.0 6 13 28 4 0.0 6 4 29 8 0.0 6 7 1 30 5 0.0 6 5 31 4 0.0 6 4 32 3 0.0 6 3 33 4 0.0 6 3 1 34 4 0.0 6 4 35 1 0.0 6 1 36 1 0.0 6 1 37 4 0.0 6 4 38 9 0.0 6 7 2 39 6 0.0 6 6 40 8 0.0 6 8 41 8 0.0 6 7 1 42 9 0.0 6 9 43 5 0.0 6 5 44 14 0.0 6 13 1 45 7 0.0 6 6 1 46 14 0.0 6 14 47 11 0.0 6 11 48 5 0.0 6 5 49 7 0.0 6 7 50 5 0.0 6 4 1 51 6 0.0 6 6 52 1 0.0 6 1 53 8 0.0 6 7 0 1 54 9 0.0 6 8 1 55 5 0.0 6 5 56 1 0.0 6 1 57 5 0.0 6 5 58 5 0.0 6 5 59 1 0.0 6 1 60 6 0.0 6 6 61 6 0.0 6 6 62 3 0.0 6 3 63 5 0.0 6 5 64 7 0.0 6 6 0 0 0 0 0 1 65 8 0.0 6 6 2 66 8 0.0 6 8 67 2 0.0 6 2 68 5 0.0 6 5 69 6 0.0 6 6 70 7 0.0 6 7 71 10 0.0 6 10 72 6 0.0 6 4 2 73 2 0.0 6 2 74 13 0.0 6 13 75 10 0.0 6 10 76 4 0.0 6 4 77 4 0.0 6 3 1 78 7 0.0 6 7 79 3 0.0 6 3 80 4 0.0 6 4 81 5 0.0 6 5 82 5 0.0 6 5 83 9 0.0 6 9 84 8 0.0 6 7 1 85 2 0.0 6 2 86 13 0.0 6 12 1 87 8 0.0 6 8 88 4 0.0 6 4 89 13 0.0 6 12 1 90 12 0.0 6 10 2 91 2 0.0 6 2 92 11 0.0 6 11 93 4 0.0 6 2 2 94 4 0.0 6 4 95 6 0.0 6 6 96 5 0.0 6 5 97 12 0.0 6 12 98 10 0.0 6 9 1 99 6 0.0 6 6 100 13 0.0 6 13 101 21 0.0 6 19 1 1 102 11 0.0 6 10 1 103 17 0.0 6 15 2 104 10 0.0 6 10 105 10 0.0 6 10 106 4 0.0 6 4 107 11 0.0 6 8 2 0 0 0 0 1 108 14 0.0 6 14 109 16 0.0 6 14 1 1 110 20 0.0 6 20 111 8 0.0 6 7 1 112 19 0.0 6 16 3 113 11 0.0 6 11 114 14 0.0 6 13 1 115 9 0.0 6 9 116 13 0.0 6 13 117 15 0.0 6 13 2 118 19 0.0 6 19 119 25 0.0 6 25 120 17 0.0 6 15 1 0 0 0 1 121 23 0.0 6 21 2 122 19 0.0 6 19 123 19 0.0 6 19 124 15 0.0 6 15 125 27 0.0 6 27 126 36 0.0 6 34 2 127 15 0.0 6 15 128 44 0.0 6 44 129 26 0.0 6 26 130 31 0.0 6 30 1 131 29 0.0 6 29 132 20 0.0 6 20 133 36 0.0 6 36 134 55 0.0 6 52 3 135 41 0.0 6 41 136 35 0.0 6 33 1 0 1 137 33 0.0 6 33 138 52 0.0 6 52 139 47 0.0 6 47 140 58 0.0 6 58 141 56 0.0 6 56 142 152 0.0 6 152 143 310 0.0 6 309 1 144 375 0.0 6 375 145 295 0.0 6 295 146 263 0.0 6 262 1 147 389 0.0 6 388 1 148 304 0.0 6 301 2 0 0 0 1 149 378 0.0 6 378 150 324 0.0 6 323 1 151 301 0.0 6 300 1 152 380 0.0 6 376 4 153 287 0.0 6 285 2 154 233 0.0 6 233 155 225 0.0 6 223 2 156 237 0.0 6 236 0 0 0 0 1 157 254 0.0 6 253 0 0 1 158 203 0.0 6 200 2 0 0 0 1 159 246 0.0 6 245 1 160 266 0.0 6 264 2 161 257 0.0 6 255 1 0 0 0 0 1 162 316 0.0 6 316 163 290 0.0 6 286 4 164 250 0.0 6 249 1 165 270 0.0 6 269 1 166 211 0.0 6 209 2 167 233 0.0 6 232 1 168 271 0.0 6 267 3 0 0 0 1 169 255 0.0 6 255 170 235 0.0 6 234 1 171 319 0.0 6 319 172 298 0.0 6 298 173 286 0.0 6 285 0 0 0 0 1 174 291 0.0 6 290 1 175 296 0.0 6 296 176 258 0.0 6 255 3 177 237 0.0 6 235 2 178 240 0.0 6 239 1 179 280 0.0 6 280 180 571 0.0 6 569 2 181 337 0.0 6 333 3 1 182 365 0.0 6 362 2 0 0 0 1 183 308 0.0 6 306 2 184 288 0.0 6 284 2 2 185 327 0.0 6 324 2 1 186 303 0.0 6 301 2 187 327 0.0 6 326 1 188 299 0.0 6 295 4 189 352 0.0 6 351 1 190 294 0.0 6 291 3 191 333 0.0 6 331 2 192 380 0.0 6 377 2 1 193 364 0.0 6 364 194 450 0.0 6 444 5 0 0 0 1 195 526 0.0 6 520 6 196 509 0.0 6 505 4 197 542 0.0 6 541 1 198 695 0.0 6 692 3 199 437 0.0 6 436 1 200 448 0.0 6 435 11 1 0 1 201 510 0.0 6 507 3 202 453 0.0 6 450 3 203 538 0.0 6 535 2 0 0 1 204 607 0.0 6 604 3 205 624 0.0 6 620 3 1 206 774 0.0 6 768 6 207 622 0.0 6 616 5 0 1 208 779 0.0 6 770 9 209 564 0.0 6 557 7 210 614 0.0 6 604 9 1 211 793 0.0 6 780 11 1 1 212 597 0.0 6 592 5 213 869 0.0 6 860 8 1 214 660 0.0 6 655 4 1 215 815 0.0 6 811 4 216 644 0.0 6 640 3 0 1 217 636 0.0 6 630 6 218 632 0.0 6 628 4 219 647 0.0 6 644 2 1 220 506 0.0 6 501 5 221 612 0.0 6 606 6 222 559 0.0 6 555 3 1 223 773 0.0 6 759 14 224 624 0.0 6 615 9 225 807 0.0 6 801 6 226 814 0.0 6 806 8 227 609 0.0 6 606 3 228 614 0.0 6 607 6 1 229 483 0.0 6 478 5 230 520 0.0 6 516 4 231 545 0.0 6 539 6 232 573 0.0 6 566 6 0 0 0 1 233 567 0.0 6 558 9 234 566 0.0 6 562 4 235 382 0.0 6 379 2 0 0 0 0 1 236 461 0.0 6 456 5 237 903 0.0 6 894 9 238 423 0.0 6 421 2 239 454 0.0 6 449 5 240 403 0.0 6 400 2 0 1 241 365 0.0 6 361 3 0 0 0 1 242 401 0.0 6 396 5 243 547 0.0 6 537 9 1 244 681 0.0 6 664 17 245 369 0.0 6 366 3 246 307 0.0 6 304 3 247 466 0.0 6 456 9 1 248 376 0.0 6 367 9 249 1110 0.0 6 1086 23 1 250 479 0.0 6 461 15 2 1 251 384 0.0 6 370 14 252 585 0.0 6 570 14 1 253 750 0.0 6 728 20 0 2 254 829 0.0 6 807 20 1 0 0 1 255 649 0.0 6 631 17 1 256 1682 0.0 6 1655 27 257 555 0.0 6 534 21 258 1587 0.0 6 1550 36 0 0 1 259 696 0.0 6 679 17 260 1659 0.0 6 1634 25 261 260 0.0 6 257 3 262 220 0.0 6 209 11 263 416 0.0 6 401 14 1 264 547 0.0 6 543 4 265 258 0.0 6 251 7 266 571 0.0 6 561 10 267 266 0.0 6 257 8 1 268 534 0.0 6 528 6 269 239 0.0 6 236 3 270 308 0.0 6 304 3 1 271 229 0.0 6 227 2 272 274 0.0 6 265 8 1 273 731 0.0 6 717 14 274 570 0.0 6 554 15 1 275 1572 0.0 6 1532 40 276 2305 0.0 6 2243 62 277 1349 0.0 6 1303 42 4 278 5705 0.0 6 5589 114 1 0 0 1 279 1222 0.0 6 1199 22 1 280 11 0.0 6 10 1 281 5 0.0 6 5 282 6 0.0 6 4 0 1 1 283 2 0.0 6 2 284 1 0.0 6 1 285 1 0.0 6 1 290 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1302 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 2 0.0 6 2 62 1 0.0 6 1 63 57 0.0 6 55 2 64 1 0.0 6 1 65 1 0.0 6 1 72 9 0.0 6 9 73 12 0.0 6 12 74 21 0.0 6 21 75 16 0.0 6 16 76 8 0.0 6 8 77 9 0.0 6 9 78 6 0.0 6 6 79 8 0.0 6 8 80 11 0.0 6 11 81 13 0.0 6 12 1 82 17 0.0 6 17 83 6 0.0 6 5 1 84 8 0.0 6 8 85 3 0.0 6 3 86 5 0.0 6 5 87 5 0.0 6 5 88 6 0.0 6 6 89 7 0.0 6 7 90 6 0.0 6 6 91 9 0.0 6 9 92 9 0.0 6 9 93 10 0.0 6 10 94 11 0.0 6 11 95 9 0.0 6 9 96 5 0.0 6 5 97 4 0.0 6 4 98 12 0.0 6 12 99 4 0.0 6 4 100 9 0.0 6 9 101 10 0.0 6 10 102 3 0.0 6 3 103 6 0.0 6 6 104 6 0.0 6 6 105 6 0.0 6 6 106 7 0.0 6 7 107 7 0.0 6 7 108 6 0.0 6 6 109 14 0.0 6 14 110 10 0.0 6 10 111 8 0.0 6 8 112 4 0.0 6 4 113 7 0.0 6 7 114 14 0.0 6 14 115 6 0.0 6 6 116 3 0.0 6 3 117 4 0.0 6 4 118 5 0.0 6 5 120 7 0.0 6 7 121 10 0.0 6 10 122 2 0.0 6 2 123 8 0.0 6 8 124 9 0.0 6 9 125 6 0.0 6 6 126 5 0.0 6 5 127 6 0.0 6 6 128 9 0.0 6 9 129 5 0.0 6 5 130 5 0.0 6 5 131 5 0.0 6 5 132 3 0.0 6 3 133 8 0.0 6 8 134 10 0.0 6 10 135 3 0.0 6 3 136 8 0.0 6 8 137 7 0.0 6 7 138 3 0.0 6 3 139 10 0.0 6 10 140 11 0.0 6 11 141 4 0.0 6 4 142 4 0.0 6 4 143 5 0.0 6 5 144 3 0.0 6 3 145 10 0.0 6 10 146 6 0.0 6 6 147 5 0.0 6 5 148 4 0.0 6 4 149 12 0.0 6 12 150 8 0.0 6 8 151 14 0.0 6 14 152 8 0.0 6 8 153 16 0.0 6 16 154 7 0.0 6 7 155 11 0.0 6 11 156 11 0.0 6 11 157 10 0.0 6 10 158 14 0.0 6 14 159 4 0.0 6 4 160 6 0.0 6 6 161 5 0.0 6 5 162 11 0.0 6 11 163 9 0.0 6 9 164 9 0.0 6 9 165 8 0.0 6 8 166 10 0.0 6 10 167 5 0.0 6 5 168 8 0.0 6 8 169 3 0.0 6 3 170 10 0.0 6 10 171 8 0.0 6 8 172 6 0.0 6 6 173 11 0.0 6 11 174 3 0.0 6 3 175 8 0.0 6 8 176 3 0.0 6 3 177 7 0.0 6 7 178 9 0.0 6 9 179 8 0.0 6 8 180 8 0.0 6 8 181 10 0.0 6 10 182 6 0.0 6 6 183 8 0.0 6 8 184 9 0.0 6 9 185 12 0.0 6 12 186 6 0.0 6 6 187 7 0.0 6 7 188 6 0.0 6 6 189 12 0.0 6 12 190 1 0.0 6 1 191 9 0.0 6 9 192 4 0.0 6 4 193 5 0.0 6 5 194 7 0.0 6 7 195 10 0.0 6 10 196 5 0.0 6 5 197 8 0.0 6 8 198 5 0.0 6 5 199 4 0.0 6 4 200 7 0.0 6 7 201 7 0.0 6 7 202 10 0.0 6 10 203 10 0.0 6 10 204 5 0.0 6 5 205 6 0.0 6 6 206 7 0.0 6 7 207 7 0.0 6 7 208 6 0.0 6 6 209 4 0.0 6 4 210 4 0.0 6 4 211 8 0.0 6 8 212 6 0.0 6 6 213 10 0.0 6 10 214 3 0.0 6 3 215 9 0.0 6 9 216 6 0.0 6 6 217 3 0.0 6 3 218 6 0.0 6 6 219 3 0.0 6 3 220 8 0.0 6 8 221 5 0.0 6 5 222 6 0.0 6 6 223 1 0.0 6 1 224 5 0.0 6 5 225 1 0.0 6 1 226 4 0.0 6 4 227 3 0.0 6 3 228 6 0.0 6 6 229 8 0.0 6 8 230 4 0.0 6 4 231 5 0.0 6 5 232 3 0.0 6 3 233 5 0.0 6 5 235 1 0.0 6 1 236 5 0.0 6 5 237 2 0.0 6 2 238 3 0.0 6 3 239 4 0.0 6 4 240 3 0.0 6 3 241 4 0.0 6 4 242 2 0.0 6 2 243 2 0.0 6 2 244 2 0.0 6 2 245 4 0.0 6 4 246 2 0.0 6 2 247 4 0.0 6 4 249 1 0.0 6 1 250 3 0.0 6 3 252 1 0.0 6 1 253 3 0.0 6 3 254 3 0.0 6 3 255 2 0.0 6 2 256 1 0.0 6 1 257 1 0.0 6 1 258 2 0.0 6 2 259 1 0.0 6 1 261 2 0.0 6 2 262 2 0.0 6 2 263 2 0.0 6 2 265 1 0.0 6 1 266 1 0.0 6 1 268 2 0.0 6 2 269 1 0.0 6 1 271 2 0.0 6 2 272 1 0.0 6 1 273 2 0.0 6 2 276 1 0.0 6 1 278 1 0.0 6 1 280 1 0.0 6 1 281 1 0.0 6 1 282 1 0.0 6 1 298 2 0.0 6 2 300 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 77475 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 76.8 1 1 11 4 0.0 3 4 12 10 0.0 3 9 0 1 13 4 0.0 3 2 2 14 14 0.0 4 14 15 2 0.0 4 1 1 16 5 0.0 4 4 1 17 21 0.0 5 21 18 18 0.0 5 17 1 19 18 0.0 5 16 2 20 69 0.0 6 67 2 21 37 0.0 6 34 3 22 31 0.0 6 29 2 23 20 0.0 6 19 1 24 24 0.0 6 22 2 25 18 0.0 6 17 1 26 23 0.0 6 22 1 27 16 0.0 6 16 28 7 0.0 6 7 29 12 0.0 6 12 30 18 0.0 6 17 1 31 10 0.0 6 9 1 32 10 0.0 6 9 1 33 8 0.0 6 8 34 15 0.0 6 14 1 35 9 0.0 6 9 36 11 0.0 6 11 37 13 0.0 6 13 38 14 0.0 6 13 1 39 10 0.0 6 10 40 7 0.0 6 7 41 11 0.0 6 11 42 10 0.0 6 8 2 43 11 0.0 6 11 44 18 0.0 6 16 2 45 12 0.0 6 11 1 46 15 0.0 6 12 2 1 47 19 0.0 6 18 1 48 17 0.0 6 16 1 49 13 0.0 6 12 1 50 21 0.0 6 20 1 51 13 0.0 6 12 1 52 22 0.0 6 21 1 53 16 0.0 6 15 1 54 21 0.0 6 18 3 55 28 0.0 6 26 2 56 17 0.0 6 17 57 24 0.0 6 24 58 17 0.0 6 16 1 59 24 0.0 6 24 60 16 0.0 6 12 3 0 1 61 17 0.0 6 17 62 16 0.0 6 13 2 1 63 20 0.0 6 20 64 25 0.0 6 24 1 65 35 0.0 6 32 3 66 21 0.0 6 20 1 67 40 0.0 6 38 2 68 141 0.0 6 130 11 69 45 0.0 6 43 2 70 72 0.0 6 68 3 0 0 0 0 1 71 37 0.0 6 34 2 0 0 0 0 1 72 72 0.0 6 67 4 0 1 73 31 0.0 6 30 1 74 23 0.0 6 20 2 0 1 75 32 0.0 6 31 0 0 1 76 42 0.0 6 37 5 77 30 0.0 6 29 0 1 78 49 0.0 6 49 79 38 0.0 6 34 4 80 53 0.0 6 49 3 1 81 35 0.0 6 34 1 82 39 0.0 6 38 0 0 0 0 1 83 65 0.0 6 61 3 0 1 84 79 0.0 6 74 3 1 0 0 0 1 85 49 0.0 6 47 2 86 50 0.0 6 46 3 1 87 54 0.0 6 54 88 38 0.0 6 37 1 89 39 0.0 6 32 7 90 62 0.0 6 60 2 91 43 0.0 6 38 4 0 1 92 61 0.0 6 57 4 93 52 0.0 6 51 1 94 108 0.0 6 102 4 2 95 56 0.0 6 56 96 57 0.0 6 53 3 1 97 73 0.0 6 72 1 98 59 0.0 6 56 2 0 1 99 79 0.0 6 78 1 100 66 0.0 6 66 101 77 0.0 6 76 1 102 77 0.0 6 74 3 103 87 0.0 6 81 6 104 100 0.0 6 96 3 0 1 105 173 0.0 6 166 7 106 80 0.0 6 76 2 1 0 0 1 107 86 0.0 6 81 4 1 108 108 0.0 6 107 0 1 109 89 0.0 6 86 3 110 80 0.0 6 77 2 0 0 0 0 1 111 90 0.0 6 86 4 112 101 0.0 6 97 2 0 2 113 113 0.0 6 107 6 114 114 0.0 6 110 3 1 115 102 0.0 6 97 5 116 120 0.0 6 116 3 0 0 0 1 117 102 0.0 6 99 3 118 157 0.0 6 154 3 119 173 0.0 6 170 2 0 0 0 1 120 139 0.0 6 135 4 121 144 0.0 6 136 7 1 122 155 0.0 6 148 6 1 123 169 0.0 6 166 3 124 206 0.0 6 203 3 125 201 0.0 6 195 4 0 1 1 126 220 0.0 6 214 2 1 0 1 0 2 127 224 0.0 6 218 4 0 0 0 1 1 128 219 0.0 6 211 8 129 243 0.0 6 236 4 0 1 1 1 130 258 0.0 6 250 7 0 0 0 0 1 131 323 0.0 6 308 14 1 132 276 0.0 6 270 6 133 331 0.0 6 322 8 0 0 0 1 134 369 0.0 6 363 5 0 0 1 135 427 0.0 6 415 11 0 0 1 136 399 0.0 6 387 11 1 137 380 0.0 6 372 8 138 394 0.0 6 392 2 139 364 0.0 6 358 6 140 562 0.0 6 558 4 141 674 0.0 6 664 9 0 1 142 830 0.0 6 809 18 3 143 779 0.0 6 769 9 0 1 144 774 0.0 6 755 16 1 0 0 1 1 145 831 0.0 6 819 11 1 146 790 0.0 6 780 8 1 0 1 147 830 0.0 6 817 10 0 2 1 148 877 0.0 6 855 18 1 0 1 0 2 149 1001 0.0 6 990 8 1 0 1 0 1 150 1123 0.0 6 1105 14 2 0 0 1 1 151 1317 0.0 6 1293 21 1 0 0 1 1 152 972 0.0 6 956 16 153 1138 0.0 6 1130 8 154 1188 0.0 6 1175 12 1 155 816 0.0 6 805 10 1 156 994 0.0 6 976 15 1 1 0 0 1 157 935 0.0 6 921 14 158 1086 0.0 6 1067 17 0 1 0 0 1 159 900 0.0 6 887 11 0 0 1 1 160 932 0.0 6 919 12 0 0 0 1 161 835 0.0 6 825 8 1 0 1 162 748 0.0 6 737 8 0 1 2 163 815 0.0 6 802 11 1 1 164 793 0.0 6 778 11 1 0 1 1 1 165 798 0.0 6 782 16 166 833 0.0 6 820 12 1 167 896 0.0 6 885 10 0 0 0 0 1 168 885 0.0 6 866 17 0 2 169 906 0.0 6 894 12 170 799 0.0 6 788 10 0 0 0 1 171 819 0.0 6 804 14 1 172 797 0.0 6 789 7 1 173 833 0.0 6 820 10 0 0 2 0 1 174 869 0.0 6 861 7 1 175 977 0.0 6 965 10 0 0 0 0 2 176 804 0.0 6 790 14 177 1078 0.0 6 1058 15 0 1 1 2 1 178 811 0.0 6 784 26 1 179 894 0.0 6 878 15 1 180 721 0.0 6 703 16 2 181 827 0.0 6 808 17 1 0 1 182 519 0.0 6 509 9 0 0 0 0 1 183 760 0.0 6 744 15 0 0 1 184 853 0.0 6 836 15 0 1 0 0 1 185 920 0.0 6 904 15 0 0 0 0 1 186 1370 0.0 6 1335 33 2 187 1011 0.0 6 986 24 0 1 188 1099 0.0 6 1078 20 0 0 1 189 686 0.0 6 664 18 3 1 190 796 0.0 6 774 20 0 1 1 191 716 0.0 6 700 14 1 0 0 0 1 192 654 0.0 6 635 18 0 0 1 193 861 0.0 6 844 17 194 409 0.0 6 400 8 1 195 696 0.0 6 679 16 1 196 338 0.0 6 333 5 197 751 0.0 6 732 19 198 1140 0.0 6 1103 34 1 0 1 0 1 199 840 0.0 6 817 21 1 0 0 1 200 593 0.0 6 576 17 201 603 0.0 6 587 16 202 595 0.0 6 579 15 0 0 0 0 1 203 522 0.0 6 509 11 0 0 1 0 1 204 399 0.0 6 384 13 1 1 205 642 0.0 6 617 24 0 0 1 206 193 0.0 6 186 5 1 1 207 432 0.0 6 413 19 208 702 0.0 6 688 13 0 0 1 209 534 0.0 6 508 24 2 210 186 0.0 6 179 6 1 211 296 0.0 6 282 13 0 0 1 212 416 0.0 6 394 20 1 1 213 516 0.0 6 506 9 0 0 1 214 265 0.0 6 257 7 1 215 315 0.0 6 311 4 216 265 0.0 6 257 8 217 210 0.0 6 206 4 218 225 0.0 6 217 8 219 153 0.0 6 146 6 0 0 1 220 109 0.0 6 106 3 221 72 0.0 6 70 2 222 144 0.0 6 137 4 2 1 223 377 0.0 6 360 17 224 701 0.0 6 678 22 0 0 1 225 451 0.0 6 440 7 4 226 378 0.0 6 359 18 0 0 1 227 1364 0.0 6 1333 30 0 0 1 228 406 0.0 6 392 14 229 787 0.0 6 763 24 230 46 0.0 6 41 5 231 155 0.0 6 151 4 232 185 0.0 6 176 8 0 1 233 287 0.0 6 275 12 234 233 0.0 6 228 5 235 144 0.0 6 140 3 0 0 1 236 71 0.0 6 68 3 237 124 0.0 6 122 2 238 108 0.0 6 107 1 239 66 0.0 6 63 3 240 18 0.0 6 17 1 241 21 0.0 6 20 1 242 231 0.0 6 221 10 243 159 0.0 6 154 5 244 97 0.0 6 94 3 245 75 0.0 6 73 2 246 90 0.0 6 89 1 247 46 0.0 6 43 3 248 98 0.0 6 94 3 1 249 161 0.0 6 157 4 250 38 0.0 6 37 1 251 89 0.0 6 87 2 252 160 0.0 6 156 4 253 15 0.0 6 14 1 254 9 0.0 6 7 2 255 2 0.0 6 2 256 1 0.0 6 1 262 1 0.0 6 1 276 1 0.0 6 1 277 2 0.0 6 2 278 2 0.0 6 2 279 17 0.0 6 17 280 67 0.0 6 63 4 281 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1170 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 6 40 19.2 1 0 0 40 48 1 0.0 6 0 1 53 2 0.0 6 2 61 1 0.0 6 1 67 1 0.0 6 1 70 2 0.0 6 2 71 2 0.0 6 2 72 13 0.0 6 13 73 22 0.0 6 22 74 18 0.0 6 18 75 6 0.0 6 6 76 17 0.0 6 17 77 15 0.0 6 15 78 13 0.0 6 13 79 13 0.0 6 13 80 11 0.0 6 11 81 18 0.0 6 18 82 6 0.0 6 6 83 9 0.0 6 9 84 6 0.0 6 6 85 10 0.0 6 8 1 0 0 1 86 9 0.0 6 9 87 8 0.0 6 8 88 8 0.0 6 8 89 5 0.0 6 5 90 12 0.0 6 11 0 0 0 1 91 9 0.0 6 9 92 8 0.0 6 8 93 7 0.0 6 7 94 9 0.0 6 9 95 4 0.0 6 4 96 3 0.0 6 3 97 10 0.0 6 10 98 6 0.0 6 5 0 0 1 99 5 0.0 6 5 100 14 0.0 6 14 101 9 0.0 6 9 102 11 0.0 6 11 103 11 0.0 6 11 104 40 0.0 6 40 105 8 0.0 6 8 106 8 0.0 6 8 107 10 0.0 6 10 108 6 0.0 6 6 109 18 0.0 6 17 0 0 0 1 110 11 0.0 6 11 111 6 0.0 6 6 112 4 0.0 6 4 113 1 0.0 6 1 114 7 0.0 6 7 115 9 0.0 6 9 116 7 0.0 6 7 117 11 0.0 6 11 118 10 0.0 6 10 119 4 0.0 6 4 120 7 0.0 6 7 121 5 0.0 6 5 122 5 0.0 6 5 123 5 0.0 6 5 124 8 0.0 6 8 125 9 0.0 6 9 126 5 0.0 6 5 127 5 0.0 6 5 128 6 0.0 6 6 129 5 0.0 6 5 130 6 0.0 6 6 131 10 0.0 6 10 132 12 0.0 6 12 133 18 0.0 6 18 134 10 0.0 6 10 135 10 0.0 6 10 136 15 0.0 6 15 137 8 0.0 6 8 138 11 0.0 6 11 139 5 0.0 6 5 140 7 0.0 6 7 141 7 0.0 6 7 142 12 0.0 6 12 143 5 0.0 6 5 144 13 0.0 6 13 145 13 0.0 6 13 146 8 0.0 6 8 147 12 0.0 6 12 148 13 0.0 6 13 149 8 0.0 6 8 150 9 0.0 6 9 151 10 0.0 6 10 152 4 0.0 6 3 0 0 0 1 153 10 0.0 6 10 154 12 0.0 6 11 0 0 1 155 9 0.0 6 9 156 7 0.0 6 7 157 12 0.0 6 12 158 3 0.0 6 3 159 8 0.0 6 8 160 7 0.0 6 7 161 9 0.0 6 9 162 11 0.0 6 11 163 15 0.0 6 15 164 8 0.0 6 8 165 11 0.0 6 11 166 11 0.0 6 11 167 9 0.0 6 9 168 9 0.0 6 9 169 5 0.0 6 5 170 7 0.0 6 7 171 8 0.0 6 8 172 4 0.0 6 4 173 10 0.0 6 9 1 174 5 0.0 6 5 175 10 0.0 6 10 176 7 0.0 6 7 177 3 0.0 6 3 178 11 0.0 6 11 179 4 0.0 6 4 180 7 0.0 6 7 181 4 0.0 6 4 182 3 0.0 6 3 183 3 0.0 6 3 184 5 0.0 6 5 185 4 0.0 6 4 186 7 0.0 6 7 187 9 0.0 6 9 188 3 0.0 6 3 189 4 0.0 6 4 190 4 0.0 6 4 191 4 0.0 6 4 192 4 0.0 6 4 193 4 0.0 6 4 194 7 0.0 6 7 195 5 0.0 6 5 196 4 0.0 6 4 197 3 0.0 6 3 198 3 0.0 6 3 199 1 0.0 6 1 200 1 0.0 6 1 201 3 0.0 6 3 202 2 0.0 6 2 203 2 0.0 6 2 204 3 0.0 6 3 205 3 0.0 6 3 208 2 0.0 6 2 209 3 0.0 6 3 210 2 0.0 6 2 211 2 0.0 6 2 212 3 0.0 6 3 213 1 0.0 6 1 215 2 0.0 6 2 217 1 0.0 6 1 225 1 0.0 6 1 226 1 0.0 6 1 231 2 0.0 6 2 232 1 0.0 6 1 238 1 0.0 6 1 253 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_09_153_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_09_380_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_09_153_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_09_380_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.59 s (78 µs/read; 0.76 M reads/minute). === Summary === Total read pairs processed: 58,547 Read 1 with adapter: 58,533 (100.0%) Read 2 with adapter: 58,528 (100.0%) == Read fate breakdown == Pairs that were too short: 33 (0.1%) Pairs discarded as untrimmed: 33 (0.1%) Pairs written (passing filters): 58,481 (99.9%) Total basepairs processed: 25,405,864 bp Read 1: 14,180,773 bp Read 2: 11,225,091 bp Total written (filtered): 3,673,065 bp (14.5%) Read 1: 1,941,712 bp Read 2: 1,731,353 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 56907 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 4 228.7 1 3 1 5 1 57.2 1 1 8 1 0.9 2 1 9 2 0.2 2 2 11 1 0.0 3 1 15 4 0.0 4 4 16 2 0.0 4 1 1 17 3 0.0 5 3 18 1 0.0 5 1 19 1 0.0 5 1 20 54 0.0 6 51 2 1 21 36 0.0 6 34 2 22 34 0.0 6 33 1 23 22 0.0 6 20 2 24 23 0.0 6 21 1 1 25 11 0.0 6 11 26 10 0.0 6 10 27 6 0.0 6 5 1 28 8 0.0 6 8 29 7 0.0 6 7 30 3 0.0 6 3 31 1 0.0 6 0 0 0 0 0 0 1 32 5 0.0 6 5 33 4 0.0 6 4 34 5 0.0 6 5 35 4 0.0 6 3 1 36 9 0.0 6 9 37 4 0.0 6 4 38 4 0.0 6 4 39 15 0.0 6 14 1 40 8 0.0 6 8 41 5 0.0 6 4 0 1 42 2 0.0 6 2 43 8 0.0 6 6 1 1 44 3 0.0 6 2 1 46 4 0.0 6 4 47 5 0.0 6 5 48 4 0.0 6 3 0 0 0 0 0 1 49 8 0.0 6 8 50 6 0.0 6 6 51 8 0.0 6 8 52 7 0.0 6 6 1 53 3 0.0 6 3 54 1 0.0 6 1 55 6 0.0 6 6 56 2 0.0 6 2 57 1 0.0 6 1 58 2 0.0 6 1 0 1 59 3 0.0 6 3 60 2 0.0 6 2 61 6 0.0 6 6 62 3 0.0 6 3 63 4 0.0 6 4 64 5 0.0 6 4 1 65 8 0.0 6 8 66 3 0.0 6 3 67 4 0.0 6 3 1 68 9 0.0 6 8 1 69 8 0.0 6 8 70 10 0.0 6 10 71 9 0.0 6 8 1 72 4 0.0 6 4 73 7 0.0 6 7 74 8 0.0 6 7 0 0 1 75 10 0.0 6 8 1 1 76 8 0.0 6 8 77 9 0.0 6 9 78 7 0.0 6 7 79 5 0.0 6 5 80 6 0.0 6 6 81 6 0.0 6 5 0 0 0 0 1 82 6 0.0 6 5 1 83 7 0.0 6 6 1 84 8 0.0 6 7 1 85 3 0.0 6 3 86 7 0.0 6 7 87 7 0.0 6 7 88 6 0.0 6 6 89 16 0.0 6 16 90 8 0.0 6 8 91 19 0.0 6 18 1 92 10 0.0 6 10 93 11 0.0 6 8 3 94 8 0.0 6 8 95 4 0.0 6 4 96 9 0.0 6 9 97 7 0.0 6 7 98 10 0.0 6 9 1 99 10 0.0 6 10 100 11 0.0 6 11 101 11 0.0 6 9 1 0 0 1 102 16 0.0 6 15 1 103 15 0.0 6 15 104 10 0.0 6 10 105 19 0.0 6 19 106 11 0.0 6 10 0 0 1 107 10 0.0 6 10 108 11 0.0 6 10 1 109 20 0.0 6 20 110 11 0.0 6 11 111 14 0.0 6 14 112 12 0.0 6 11 1 113 14 0.0 6 14 114 18 0.0 6 16 2 115 17 0.0 6 17 116 10 0.0 6 10 117 19 0.0 6 17 2 118 9 0.0 6 9 119 18 0.0 6 18 120 5 0.0 6 5 121 14 0.0 6 14 122 14 0.0 6 12 2 123 18 0.0 6 17 1 124 22 0.0 6 22 125 31 0.0 6 30 0 1 126 24 0.0 6 24 127 18 0.0 6 17 1 128 19 0.0 6 19 129 33 0.0 6 30 2 1 130 24 0.0 6 22 2 131 19 0.0 6 19 132 25 0.0 6 24 1 133 24 0.0 6 24 134 21 0.0 6 21 135 37 0.0 6 37 136 25 0.0 6 23 2 137 22 0.0 6 19 2 0 0 0 0 1 138 26 0.0 6 26 139 46 0.0 6 46 140 143 0.0 6 141 2 141 132 0.0 6 131 1 142 247 0.0 6 245 2 143 148 0.0 6 148 144 94 0.0 6 93 1 145 435 0.0 6 432 2 0 0 0 0 1 146 290 0.0 6 287 2 0 1 147 194 0.0 6 193 1 148 167 0.0 6 165 2 149 303 0.0 6 303 150 288 0.0 6 287 0 0 0 0 1 151 225 0.0 6 224 0 0 1 152 205 0.0 6 205 153 131 0.0 6 130 0 0 0 0 1 154 172 0.0 6 172 155 163 0.0 6 162 0 0 0 1 156 87 0.0 6 85 2 157 318 0.0 6 317 1 158 110 0.0 6 110 159 276 0.0 6 274 2 160 330 0.0 6 326 4 161 151 0.0 6 151 162 165 0.0 6 164 1 163 199 0.0 6 199 164 174 0.0 6 172 2 165 172 0.0 6 168 4 166 153 0.0 6 149 4 167 232 0.0 6 229 3 168 369 0.0 6 368 1 169 266 0.0 6 266 170 164 0.0 6 162 0 0 0 0 0 2 171 151 0.0 6 149 1 0 1 172 184 0.0 6 181 3 173 192 0.0 6 190 2 174 209 0.0 6 206 3 175 222 0.0 6 220 2 176 187 0.0 6 184 2 0 1 177 166 0.0 6 164 2 178 267 0.0 6 265 1 1 179 206 0.0 6 203 3 180 233 0.0 6 233 181 220 0.0 6 220 182 483 0.0 6 480 3 183 314 0.0 6 312 2 184 314 0.0 6 311 2 0 0 0 1 185 249 0.0 6 246 3 186 284 0.0 6 282 2 187 601 0.0 6 597 4 188 263 0.0 6 261 1 0 0 1 189 253 0.0 6 251 2 190 638 0.0 6 637 1 191 309 0.0 6 304 4 0 1 192 354 0.0 6 353 1 193 342 0.0 6 341 1 194 771 0.0 6 764 6 0 0 0 1 195 656 0.0 6 651 4 0 0 0 0 1 196 1120 0.0 6 1114 5 1 197 710 0.0 6 701 9 198 779 0.0 6 776 3 199 891 0.0 6 885 5 1 200 657 0.0 6 650 7 201 628 0.0 6 623 4 0 0 0 0 1 202 990 0.0 6 982 8 203 619 0.0 6 615 4 204 548 0.0 6 541 4 1 2 205 601 0.0 6 592 9 206 694 0.0 6 694 207 685 0.0 6 680 5 208 705 0.0 6 698 7 209 559 0.0 6 554 3 0 2 210 592 0.0 6 587 3 0 0 1 1 211 1049 0.0 6 1044 5 212 824 0.0 6 818 4 1 0 0 0 1 213 730 0.0 6 720 10 214 886 0.0 6 882 4 215 1073 0.0 6 1067 5 0 0 1 216 879 0.0 6 874 5 217 803 0.0 6 801 2 218 855 0.0 6 849 6 219 671 0.0 6 663 8 220 576 0.0 6 573 2 1 221 511 0.0 6 504 7 222 624 0.0 6 616 8 223 839 0.0 6 833 6 224 652 0.0 6 651 1 225 912 0.0 6 907 4 1 226 875 0.0 6 869 3 0 1 1 1 227 483 0.0 6 481 2 228 460 0.0 6 459 1 229 485 0.0 6 480 4 0 0 0 0 1 230 880 0.0 6 875 3 1 1 231 462 0.0 6 458 3 1 232 475 0.0 6 472 3 233 400 0.0 6 398 2 234 410 0.0 6 406 4 235 314 0.0 6 313 1 236 412 0.0 6 410 2 237 438 0.0 6 429 9 238 488 0.0 6 481 6 0 1 239 430 0.0 6 427 3 240 411 0.0 6 407 4 241 410 0.0 6 408 2 242 372 0.0 6 368 4 243 349 0.0 6 346 3 244 347 0.0 6 341 5 0 0 0 1 245 275 0.0 6 272 3 246 266 0.0 6 264 2 247 323 0.0 6 322 1 248 343 0.0 6 341 2 249 244 0.0 6 243 1 250 239 0.0 6 234 5 251 227 0.0 6 224 3 252 205 0.0 6 203 2 253 208 0.0 6 204 4 254 210 0.0 6 208 1 1 255 200 0.0 6 196 2 1 0 0 1 256 218 0.0 6 217 1 257 189 0.0 6 187 2 258 172 0.0 6 171 1 259 230 0.0 6 229 1 260 204 0.0 6 203 1 261 125 0.0 6 125 262 139 0.0 6 137 2 263 180 0.0 6 178 1 0 0 1 264 138 0.0 6 136 2 265 132 0.0 6 130 2 266 91 0.0 6 90 0 0 0 0 1 267 130 0.0 6 128 2 268 97 0.0 6 96 1 269 137 0.0 6 134 3 270 125 0.0 6 124 1 271 102 0.0 6 99 3 272 115 0.0 6 112 2 1 273 102 0.0 6 101 1 274 157 0.0 6 156 1 275 139 0.0 6 138 0 0 0 0 1 276 225 0.0 6 222 3 277 316 0.0 6 308 8 278 974 0.0 6 961 12 0 1 279 2169 0.0 6 2134 33 1 0 0 0 1 280 13 0.0 6 10 3 281 2 0.0 6 2 283 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1626 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 1 0.0 6 1 72 6 0.0 6 6 73 6 0.0 6 6 74 9 0.0 6 8 1 75 3 0.0 6 3 76 14 0.0 6 14 77 17 0.0 6 17 78 6 0.0 6 6 79 6 0.0 6 6 80 6 0.0 6 6 81 10 0.0 6 10 82 11 0.0 6 11 83 13 0.0 6 13 84 7 0.0 6 7 85 5 0.0 6 5 86 6 0.0 6 6 87 1 0.0 6 1 88 5 0.0 6 5 89 9 0.0 6 9 90 3 0.0 6 3 91 11 0.0 6 11 92 2 0.0 6 2 93 1 0.0 6 1 95 4 0.0 6 4 96 4 0.0 6 4 97 3 0.0 6 2 1 98 3 0.0 6 3 99 10 0.0 6 10 100 14 0.0 6 14 101 3 0.0 6 3 102 2 0.0 6 2 103 2 0.0 6 2 104 4 0.0 6 4 105 6 0.0 6 5 1 106 3 0.0 6 3 107 4 0.0 6 4 109 2 0.0 6 2 110 5 0.0 6 5 111 3 0.0 6 3 112 8 0.0 6 8 113 8 0.0 6 8 114 16 0.0 6 16 115 7 0.0 6 7 117 4 0.0 6 4 118 10 0.0 6 10 119 9 0.0 6 9 120 4 0.0 6 4 121 8 0.0 6 8 122 14 0.0 6 14 123 2 0.0 6 2 124 3 0.0 6 3 125 9 0.0 6 9 126 28 0.0 6 28 127 14 0.0 6 14 128 24 0.0 6 24 129 3 0.0 6 3 130 6 0.0 6 6 131 24 0.0 6 24 132 14 0.0 6 14 133 22 0.0 6 22 134 33 0.0 6 33 135 13 0.0 6 13 136 2 0.0 6 2 137 1 0.0 6 1 138 7 0.0 6 7 139 7 0.0 6 7 140 8 0.0 6 8 141 16 0.0 6 16 142 19 0.0 6 18 1 143 27 0.0 6 27 144 16 0.0 6 16 145 5 0.0 6 5 146 8 0.0 6 8 147 12 0.0 6 12 148 31 0.0 6 31 149 13 0.0 6 12 1 150 5 0.0 6 5 151 7 0.0 6 7 152 4 0.0 6 4 153 4 0.0 6 4 154 12 0.0 6 12 155 5 0.0 6 5 156 9 0.0 6 9 157 15 0.0 6 15 158 5 0.0 6 5 159 5 0.0 6 5 160 5 0.0 6 5 161 3 0.0 6 3 162 3 0.0 6 3 163 7 0.0 6 7 164 10 0.0 6 10 165 7 0.0 6 7 166 8 0.0 6 7 1 167 5 0.0 6 5 168 5 0.0 6 5 169 9 0.0 6 9 170 18 0.0 6 18 171 6 0.0 6 6 172 8 0.0 6 8 173 15 0.0 6 15 174 5 0.0 6 5 175 7 0.0 6 7 176 19 0.0 6 19 177 12 0.0 6 12 178 15 0.0 6 15 179 19 0.0 6 19 180 22 0.0 6 22 182 1 0.0 6 1 183 11 0.0 6 11 184 10 0.0 6 10 185 13 0.0 6 13 186 4 0.0 6 4 187 8 0.0 6 8 188 37 0.0 6 37 189 15 0.0 6 15 190 27 0.0 6 27 191 49 0.0 6 49 192 15 0.0 6 15 193 2 0.0 6 2 194 19 0.0 6 19 195 43 0.0 6 43 196 10 0.0 6 10 197 4 0.0 6 4 198 11 0.0 6 11 199 7 0.0 6 7 200 7 0.0 6 7 201 8 0.0 6 8 202 12 0.0 6 12 203 19 0.0 6 19 204 12 0.0 6 12 205 14 0.0 6 14 206 6 0.0 6 6 207 2 0.0 6 2 208 3 0.0 6 3 209 6 0.0 6 6 210 11 0.0 6 11 211 10 0.0 6 10 212 12 0.0 6 12 213 9 0.0 6 9 214 16 0.0 6 16 215 17 0.0 6 17 216 11 0.0 6 11 217 12 0.0 6 12 218 12 0.0 6 12 219 4 0.0 6 4 220 4 0.0 6 4 221 4 0.0 6 4 222 1 0.0 6 1 224 4 0.0 6 4 225 3 0.0 6 3 226 7 0.0 6 7 227 8 0.0 6 8 228 5 0.0 6 5 229 3 0.0 6 3 230 6 0.0 6 6 231 2 0.0 6 2 232 4 0.0 6 4 233 2 0.0 6 2 234 5 0.0 6 5 235 6 0.0 6 6 236 1 0.0 6 1 237 3 0.0 6 3 238 2 0.0 6 2 239 1 0.0 6 1 240 2 0.0 6 2 241 5 0.0 6 5 242 13 0.0 6 13 243 5 0.0 6 5 246 4 0.0 6 4 247 4 0.0 6 4 248 2 0.0 6 2 249 6 0.0 6 6 250 5 0.0 6 5 251 5 0.0 6 5 253 3 0.0 6 3 254 10 0.0 6 10 255 4 0.0 6 4 256 3 0.0 6 3 257 7 0.0 6 7 260 2 0.0 6 2 264 4 0.0 6 4 266 1 0.0 6 1 268 1 0.0 6 1 270 4 0.0 6 4 271 1 0.0 6 1 274 1 0.0 6 1 276 1 0.0 6 1 279 2 0.0 6 2 283 1 0.0 6 1 284 1 0.0 6 1 286 1 0.0 6 1 289 2 0.0 6 2 290 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 57142 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 14.3 1 1 9 1 0.2 2 1 11 2 0.0 3 2 12 4 0.0 3 4 13 4 0.0 3 4 14 9 0.0 4 9 15 1 0.0 4 1 16 7 0.0 4 4 3 17 16 0.0 5 15 1 18 1 0.0 5 1 19 46 0.0 5 40 5 0 1 20 68 0.0 6 65 3 21 47 0.0 6 43 4 22 25 0.0 6 24 1 23 23 0.0 6 22 1 24 26 0.0 6 24 2 25 12 0.0 6 12 26 15 0.0 6 13 2 27 13 0.0 6 11 2 28 10 0.0 6 8 1 0 1 29 7 0.0 6 7 30 6 0.0 6 6 31 14 0.0 6 12 2 32 9 0.0 6 9 33 7 0.0 6 4 3 34 12 0.0 6 10 0 0 1 0 1 35 6 0.0 6 4 2 36 9 0.0 6 9 37 10 0.0 6 8 1 0 1 38 7 0.0 6 7 39 8 0.0 6 7 0 0 0 1 40 6 0.0 6 5 1 41 14 0.0 6 14 42 16 0.0 6 12 4 43 14 0.0 6 12 1 0 1 44 10 0.0 6 10 45 4 0.0 6 3 1 46 14 0.0 6 12 2 47 10 0.0 6 9 1 48 17 0.0 6 15 1 0 0 0 1 49 12 0.0 6 9 2 0 0 1 50 13 0.0 6 13 51 17 0.0 6 13 2 1 1 52 21 0.0 6 20 1 53 29 0.0 6 24 4 0 1 54 16 0.0 6 13 2 0 0 1 55 21 0.0 6 21 56 19 0.0 6 17 1 0 0 0 1 57 13 0.0 6 11 1 1 58 24 0.0 6 22 2 59 13 0.0 6 13 60 8 0.0 6 7 0 0 1 61 29 0.0 6 25 4 62 21 0.0 6 20 0 0 0 1 63 16 0.0 6 14 2 64 8 0.0 6 8 65 28 0.0 6 27 1 66 31 0.0 6 26 2 2 1 67 30 0.0 6 26 3 0 0 1 68 20 0.0 6 16 3 0 0 0 1 69 28 0.0 6 26 1 1 70 111 0.0 6 105 5 0 0 1 71 49 0.0 6 42 6 0 0 0 0 1 72 43 0.0 6 41 2 73 34 0.0 6 31 1 0 1 0 1 74 36 0.0 6 34 1 0 1 75 33 0.0 6 33 76 27 0.0 6 26 1 77 29 0.0 6 26 3 78 36 0.0 6 31 2 1 0 1 1 79 24 0.0 6 22 2 80 45 0.0 6 41 3 1 81 43 0.0 6 40 2 0 0 1 82 47 0.0 6 43 3 1 83 55 0.0 6 52 3 84 47 0.0 6 44 3 85 60 0.0 6 56 4 86 62 0.0 6 60 2 87 36 0.0 6 34 2 88 33 0.0 6 29 2 1 1 89 41 0.0 6 39 2 90 36 0.0 6 35 0 0 0 0 0 1 91 37 0.0 6 35 2 92 37 0.0 6 34 2 0 0 1 93 54 0.0 6 52 1 0 0 0 0 1 94 50 0.0 6 47 3 95 49 0.0 6 42 5 1 1 96 58 0.0 6 55 3 97 47 0.0 6 41 4 1 1 98 72 0.0 6 71 1 99 82 0.0 6 76 5 1 100 69 0.0 6 64 4 1 101 51 0.0 6 51 102 55 0.0 6 52 3 103 73 0.0 6 72 1 104 82 0.0 6 80 1 1 105 67 0.0 6 64 1 1 0 1 106 88 0.0 6 85 3 107 155 0.0 6 144 10 0 0 0 1 108 77 0.0 6 76 1 109 99 0.0 6 93 5 0 1 110 91 0.0 6 85 6 111 83 0.0 6 79 4 112 87 0.0 6 85 1 1 113 109 0.0 6 104 5 114 131 0.0 6 122 8 0 0 1 115 134 0.0 6 127 7 116 113 0.0 6 108 4 0 0 0 0 1 117 95 0.0 6 92 1 0 0 0 2 118 129 0.0 6 126 2 0 0 0 1 119 139 0.0 6 135 3 0 0 1 120 144 0.0 6 140 4 121 149 0.0 6 141 5 0 2 1 122 146 0.0 6 140 5 0 1 123 141 0.0 6 135 6 124 125 0.0 6 121 3 1 125 136 0.0 6 130 4 1 0 0 1 126 170 0.0 6 167 3 127 192 0.0 6 182 6 1 1 0 0 2 128 291 0.0 6 281 10 129 198 0.0 6 190 6 0 0 0 1 1 130 215 0.0 6 210 5 131 240 0.0 6 237 3 132 238 0.0 6 233 5 133 265 0.0 6 253 9 0 1 1 0 1 134 331 0.0 6 324 7 135 315 0.0 6 304 8 1 2 136 372 0.0 6 364 8 137 391 0.0 6 385 5 0 1 138 369 0.0 6 358 9 0 0 0 1 1 139 374 0.0 6 364 7 3 140 441 0.0 6 427 13 0 0 1 141 487 0.0 6 472 14 0 0 1 142 563 0.0 6 558 5 143 499 0.0 6 490 8 1 144 513 0.0 6 501 10 0 1 0 0 1 145 836 0.0 6 820 14 0 1 0 0 1 146 661 0.0 6 648 9 0 1 1 0 2 147 641 0.0 6 624 14 0 1 0 2 148 669 0.0 6 655 13 0 1 149 792 0.0 6 776 15 0 0 1 150 718 0.0 6 701 13 2 0 1 1 151 1006 0.0 6 990 13 2 0 0 1 152 929 0.0 6 909 18 1 0 0 1 153 1125 0.0 6 1099 24 0 0 0 1 1 154 956 0.0 6 935 16 1 1 2 0 1 155 1037 0.0 6 1024 12 0 1 156 1037 0.0 6 1019 17 1 157 943 0.0 6 925 16 2 158 891 0.0 6 880 8 1 0 0 1 1 159 870 0.0 6 851 18 0 0 1 160 976 0.0 6 967 8 0 0 1 161 848 0.0 6 828 17 0 1 1 1 162 815 0.0 6 802 10 2 1 163 861 0.0 6 850 9 1 0 1 164 754 0.0 6 744 10 165 669 0.0 6 657 11 1 166 793 0.0 6 778 15 167 738 0.0 6 728 9 0 0 1 168 731 0.0 6 721 7 0 1 0 0 2 169 853 0.0 6 842 7 1 0 1 0 2 170 699 0.0 6 691 8 171 845 0.0 6 833 10 0 1 1 172 764 0.0 6 752 9 1 2 173 813 0.0 6 804 9 174 812 0.0 6 799 10 1 1 0 0 1 175 786 0.0 6 772 11 1 1 0 1 176 762 0.0 6 756 5 1 177 778 0.0 6 771 5 1 1 178 740 0.0 6 732 7 1 179 902 0.0 6 894 7 0 1 180 600 0.0 6 593 5 0 1 1 181 712 0.0 6 700 11 0 0 1 182 628 0.0 6 624 2 2 183 593 0.0 6 587 3 1 0 1 1 184 509 0.0 6 501 8 185 500 0.0 6 499 1 186 696 0.0 6 688 6 0 2 187 983 0.0 6 977 5 0 0 1 188 835 0.0 6 826 8 0 0 1 189 598 0.0 6 594 3 0 0 1 190 697 0.0 6 690 6 0 0 1 191 573 0.0 6 568 4 0 0 1 192 497 0.0 6 489 7 1 193 493 0.0 6 485 8 194 631 0.0 6 625 6 195 363 0.0 6 356 6 0 0 0 0 1 196 395 0.0 6 389 5 0 1 197 382 0.0 6 381 1 198 338 0.0 6 334 4 199 522 0.0 6 516 5 0 0 1 200 349 0.0 6 343 6 201 356 0.0 6 356 202 303 0.0 6 297 6 203 252 0.0 6 250 1 1 204 269 0.0 6 268 1 205 307 0.0 6 302 5 206 211 0.0 6 211 207 175 0.0 6 173 2 208 232 0.0 6 229 3 209 203 0.0 6 202 1 210 174 0.0 6 171 3 211 179 0.0 6 178 1 212 165 0.0 6 163 2 213 74 0.0 6 73 1 214 107 0.0 6 106 1 215 102 0.0 6 101 0 1 216 172 0.0 6 172 217 110 0.0 6 108 2 218 96 0.0 6 95 1 219 100 0.0 6 99 1 220 69 0.0 6 68 1 221 100 0.0 6 99 1 222 60 0.0 6 60 223 46 0.0 6 46 224 83 0.0 6 82 1 225 93 0.0 6 91 0 0 0 2 226 48 0.0 6 46 2 227 38 0.0 6 36 2 228 40 0.0 6 40 229 36 0.0 6 34 2 230 30 0.0 6 28 1 1 231 23 0.0 6 23 232 40 0.0 6 39 1 233 20 0.0 6 20 234 22 0.0 6 21 1 235 11 0.0 6 10 1 236 2 0.0 6 2 237 9 0.0 6 9 238 9 0.0 6 8 1 239 10 0.0 6 10 240 6 0.0 6 6 241 11 0.0 6 11 242 8 0.0 6 8 243 7 0.0 6 7 244 12 0.0 6 12 245 12 0.0 6 12 246 1 0.0 6 1 247 4 0.0 6 4 248 6 0.0 6 6 249 6 0.0 6 5 1 251 5 0.0 6 5 252 1 0.0 6 1 253 2 0.0 6 2 254 2 0.0 6 2 255 2 0.0 6 2 256 1 0.0 6 1 280 5 0.0 6 4 1 281 14 0.0 6 14 282 124 0.0 6 123 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1386 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 49 1 0.0 6 1 50 1 0.0 6 1 66 1 0.0 6 1 69 5 0.0 6 5 70 1 0.0 6 1 71 9 0.0 6 9 72 7 0.0 6 7 73 4 0.0 6 4 74 21 0.0 6 21 75 12 0.0 6 12 76 11 0.0 6 11 77 5 0.0 6 5 78 4 0.0 6 4 79 8 0.0 6 7 0 0 0 1 80 11 0.0 6 11 81 8 0.0 6 8 82 5 0.0 6 5 83 5 0.0 6 5 84 5 0.0 6 5 85 7 0.0 6 7 86 16 0.0 6 16 87 1 0.0 6 1 88 10 0.0 6 10 89 16 0.0 6 15 0 0 1 90 3 0.0 6 3 91 5 0.0 6 5 92 3 0.0 6 3 93 6 0.0 6 6 94 6 0.0 6 6 95 3 0.0 6 3 96 2 0.0 6 2 97 18 0.0 6 18 98 9 0.0 6 9 100 4 0.0 6 3 0 0 1 101 2 0.0 6 2 102 2 0.0 6 2 103 8 0.0 6 8 104 4 0.0 6 4 105 2 0.0 6 2 106 4 0.0 6 3 0 0 1 107 9 0.0 6 9 108 3 0.0 6 3 109 5 0.0 6 5 110 4 0.0 6 4 111 16 0.0 6 16 112 6 0.0 6 6 113 5 0.0 6 5 114 5 0.0 6 5 115 7 0.0 6 7 116 12 0.0 6 12 117 3 0.0 6 3 118 4 0.0 6 4 119 23 0.0 6 23 120 1 0.0 6 1 121 5 0.0 6 5 122 7 0.0 6 7 123 24 0.0 6 24 124 14 0.0 6 14 125 40 0.0 6 40 126 3 0.0 6 3 127 8 0.0 6 8 128 30 0.0 6 30 129 13 0.0 6 13 130 14 0.0 6 14 131 42 0.0 6 42 132 16 0.0 6 16 133 10 0.0 6 10 134 5 0.0 6 5 135 4 0.0 6 4 136 13 0.0 6 12 0 0 0 1 137 6 0.0 6 6 138 10 0.0 6 10 139 9 0.0 6 9 140 42 0.0 6 42 141 18 0.0 6 18 142 4 0.0 6 4 143 10 0.0 6 10 144 12 0.0 6 12 145 33 0.0 6 33 146 20 0.0 6 20 147 11 0.0 6 11 148 4 0.0 6 4 149 9 0.0 6 9 150 7 0.0 6 7 151 9 0.0 6 9 152 10 0.0 6 10 153 7 0.0 6 7 154 12 0.0 6 12 155 1 0.0 6 1 156 5 0.0 6 5 157 6 0.0 6 6 158 9 0.0 6 9 159 1 0.0 6 1 160 9 0.0 6 9 161 8 0.0 6 8 162 13 0.0 6 13 163 4 0.0 6 4 164 5 0.0 6 5 165 11 0.0 6 11 166 9 0.0 6 9 167 12 0.0 6 12 168 7 0.0 6 7 169 3 0.0 6 3 170 7 0.0 6 7 171 5 0.0 6 5 172 4 0.0 6 4 173 8 0.0 6 8 174 8 0.0 6 8 175 21 0.0 6 21 176 24 0.0 6 24 177 33 0.0 6 33 179 2 0.0 6 2 180 7 0.0 6 7 181 15 0.0 6 14 0 0 0 1 182 17 0.0 6 17 183 4 0.0 6 4 184 5 0.0 6 5 185 29 0.0 6 29 186 3 0.0 6 3 187 11 0.0 6 11 188 45 0.0 6 44 0 0 0 1 189 14 0.0 6 14 190 1 0.0 6 1 191 17 0.0 6 17 192 51 0.0 6 50 1 193 7 0.0 6 7 194 9 0.0 6 9 195 2 0.0 6 2 196 2 0.0 6 2 197 1 0.0 6 1 198 6 0.0 6 6 199 3 0.0 6 3 200 1 0.0 6 1 201 4 0.0 6 4 202 4 0.0 6 4 203 5 0.0 6 5 204 1 0.0 6 1 205 1 0.0 6 1 206 1 0.0 6 1 207 4 0.0 6 4 208 4 0.0 6 4 209 3 0.0 6 3 210 6 0.0 6 6 211 7 0.0 6 7 212 17 0.0 6 17 213 4 0.0 6 4 214 4 0.0 6 3 0 0 0 1 215 5 0.0 6 5 216 3 0.0 6 3 218 3 0.0 6 3 222 1 0.0 6 1 224 2 0.0 6 2 238 1 0.0 6 1 242 1 0.0 6 1 243 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_10_154_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_10_381_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_10_154_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_10_381_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.17 s (80 µs/read; 0.75 M reads/minute). === Summary === Total read pairs processed: 52,209 Read 1 with adapter: 52,200 (100.0%) Read 2 with adapter: 52,183 (100.0%) == Read fate breakdown == Pairs that were too short: 2 (0.0%) Pairs discarded as untrimmed: 35 (0.1%) Pairs written (passing filters): 52,172 (99.9%) Total basepairs processed: 24,316,760 bp Read 1: 13,623,633 bp Read 2: 10,693,127 bp Total written (filtered): 3,391,909 bp (13.9%) Read 1: 1,701,876 bp Read 2: 1,690,033 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 51658 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 815.8 0 1 4 1 203.9 1 1 6 2 12.7 1 0 0 2 7 4 3.2 2 0 0 4 8 1 0.8 2 1 9 1 0.2 2 1 11 1 0.0 3 1 15 2 0.0 4 2 16 1 0.0 4 1 17 3 0.0 5 2 1 18 1 0.0 5 1 19 1 0.0 5 1 20 28 0.0 6 28 21 9 0.0 6 8 1 22 16 0.0 6 15 1 23 9 0.0 6 9 24 8 0.0 6 7 1 25 6 0.0 6 6 26 5 0.0 6 5 27 5 0.0 6 4 1 28 5 0.0 6 5 29 7 0.0 6 6 1 30 3 0.0 6 3 31 1 0.0 6 1 32 5 0.0 6 4 1 33 6 0.0 6 4 2 34 5 0.0 6 5 35 1 0.0 6 1 36 2 0.0 6 2 37 3 0.0 6 3 38 4 0.0 6 3 1 39 3 0.0 6 3 40 6 0.0 6 6 41 6 0.0 6 6 42 4 0.0 6 4 43 1 0.0 6 1 44 5 0.0 6 5 45 2 0.0 6 1 1 46 3 0.0 6 3 47 3 0.0 6 3 48 3 0.0 6 3 49 2 0.0 6 1 1 50 4 0.0 6 4 51 3 0.0 6 3 52 5 0.0 6 4 1 53 7 0.0 6 7 54 4 0.0 6 3 1 55 2 0.0 6 2 56 3 0.0 6 1 2 57 4 0.0 6 4 58 3 0.0 6 3 59 4 0.0 6 4 60 2 0.0 6 1 1 61 3 0.0 6 2 1 62 4 0.0 6 4 63 1 0.0 6 1 64 7 0.0 6 7 65 5 0.0 6 5 66 3 0.0 6 3 67 2 0.0 6 2 68 2 0.0 6 2 69 3 0.0 6 2 0 0 0 1 70 3 0.0 6 3 71 3 0.0 6 3 72 5 0.0 6 5 73 3 0.0 6 3 74 3 0.0 6 3 75 5 0.0 6 5 76 3 0.0 6 3 77 4 0.0 6 4 78 5 0.0 6 5 79 5 0.0 6 5 80 3 0.0 6 3 81 5 0.0 6 5 82 4 0.0 6 4 83 3 0.0 6 3 84 5 0.0 6 5 85 5 0.0 6 5 86 8 0.0 6 7 0 0 1 87 6 0.0 6 5 1 88 8 0.0 6 8 89 8 0.0 6 8 90 8 0.0 6 8 91 4 0.0 6 4 92 4 0.0 6 4 93 9 0.0 6 9 94 4 0.0 6 3 0 0 0 0 0 1 95 6 0.0 6 6 96 3 0.0 6 3 97 1 0.0 6 1 98 6 0.0 6 6 99 2 0.0 6 2 100 12 0.0 6 12 101 6 0.0 6 6 102 10 0.0 6 6 2 1 1 103 11 0.0 6 11 104 8 0.0 6 7 1 105 10 0.0 6 9 1 106 8 0.0 6 8 107 9 0.0 6 9 108 9 0.0 6 9 109 14 0.0 6 13 0 0 0 0 0 1 110 12 0.0 6 12 111 8 0.0 6 8 112 20 0.0 6 19 1 113 11 0.0 6 10 1 114 12 0.0 6 11 1 115 19 0.0 6 18 1 116 12 0.0 6 11 0 0 1 117 11 0.0 6 11 118 11 0.0 6 11 119 16 0.0 6 16 120 11 0.0 6 11 121 18 0.0 6 18 122 19 0.0 6 18 1 123 23 0.0 6 22 0 1 124 18 0.0 6 18 125 16 0.0 6 16 126 20 0.0 6 20 127 17 0.0 6 17 128 28 0.0 6 26 1 1 129 27 0.0 6 25 2 130 33 0.0 6 33 131 28 0.0 6 27 0 0 0 0 1 132 22 0.0 6 22 133 29 0.0 6 29 134 34 0.0 6 34 135 21 0.0 6 21 136 29 0.0 6 29 137 25 0.0 6 25 138 26 0.0 6 26 139 21 0.0 6 20 1 140 32 0.0 6 32 141 34 0.0 6 34 142 53 0.0 6 50 3 143 96 0.0 6 95 1 144 214 0.0 6 212 1 1 145 250 0.0 6 250 146 313 0.0 6 313 147 251 0.0 6 251 148 334 0.0 6 331 2 1 149 401 0.0 6 400 0 1 150 424 0.0 6 423 1 151 322 0.0 6 321 1 152 366 0.0 6 366 153 436 0.0 6 435 1 154 347 0.0 6 347 155 267 0.0 6 267 156 276 0.0 6 274 0 1 0 0 1 157 263 0.0 6 262 1 158 235 0.0 6 235 159 188 0.0 6 187 1 160 241 0.0 6 240 1 161 310 0.0 6 310 162 369 0.0 6 367 2 163 329 0.0 6 329 164 345 0.0 6 345 165 310 0.0 6 309 1 166 247 0.0 6 247 167 288 0.0 6 288 168 238 0.0 6 238 169 185 0.0 6 184 1 170 234 0.0 6 233 1 171 213 0.0 6 212 1 172 241 0.0 6 240 1 173 181 0.0 6 179 2 174 203 0.0 6 203 175 238 0.0 6 238 176 182 0.0 6 180 2 177 166 0.0 6 166 178 144 0.0 6 141 2 1 179 143 0.0 6 142 1 180 182 0.0 6 182 181 289 0.0 6 287 2 182 175 0.0 6 175 183 160 0.0 6 159 1 184 172 0.0 6 170 2 185 185 0.0 6 183 2 186 190 0.0 6 188 2 187 190 0.0 6 190 188 226 0.0 6 226 189 224 0.0 6 221 3 190 153 0.0 6 153 191 211 0.0 6 211 192 183 0.0 6 181 2 193 212 0.0 6 208 4 194 270 0.0 6 269 1 195 289 0.0 6 286 3 196 344 0.0 6 340 3 1 197 237 0.0 6 235 2 198 340 0.0 6 336 4 199 200 0.0 6 198 2 200 177 0.0 6 175 2 201 227 0.0 6 223 4 202 212 0.0 6 207 5 203 224 0.0 6 219 5 204 218 0.0 6 215 3 205 317 0.0 6 313 4 206 284 0.0 6 283 1 207 281 0.0 6 275 6 208 307 0.0 6 304 2 1 209 266 0.0 6 259 6 0 0 0 0 1 210 211 0.0 6 209 2 211 188 0.0 6 185 3 212 219 0.0 6 217 2 213 196 0.0 6 194 2 214 232 0.0 6 227 5 215 280 0.0 6 279 1 216 210 0.0 6 207 3 217 296 0.0 6 294 2 218 272 0.0 6 268 3 0 0 0 1 219 172 0.0 6 170 1 1 220 175 0.0 6 174 1 221 182 0.0 6 179 3 222 244 0.0 6 242 2 223 143 0.0 6 140 2 1 224 152 0.0 6 150 2 225 227 0.0 6 220 7 226 387 0.0 6 378 9 227 288 0.0 6 278 10 228 230 0.0 6 228 2 229 226 0.0 6 221 5 230 186 0.0 6 183 2 1 231 216 0.0 6 214 1 1 232 180 0.0 6 176 4 233 204 0.0 6 201 3 234 166 0.0 6 163 3 235 149 0.0 6 147 2 236 134 0.0 6 133 1 237 197 0.0 6 195 2 238 160 0.0 6 157 3 239 240 0.0 6 237 3 240 224 0.0 6 218 6 241 484 0.0 6 473 10 1 242 380 0.0 6 373 7 243 380 0.0 6 375 5 244 230 0.0 6 229 1 245 494 0.0 6 489 5 246 114 0.0 6 111 2 0 1 247 190 0.0 6 186 4 248 117 0.0 6 111 5 1 249 327 0.0 6 322 5 250 274 0.0 6 271 3 251 268 0.0 6 260 8 252 208 0.0 6 205 3 253 167 0.0 6 162 5 254 409 0.0 6 403 6 255 226 0.0 6 223 3 256 168 0.0 6 165 2 1 257 235 0.0 6 227 7 1 258 349 0.0 6 344 5 259 366 0.0 6 360 5 1 260 600 0.0 6 591 8 1 261 211 0.0 6 204 7 262 228 0.0 6 225 3 263 551 0.0 6 542 9 264 234 0.0 6 231 3 265 332 0.0 6 328 4 266 106 0.0 6 104 1 1 267 306 0.0 6 301 5 268 257 0.0 6 252 5 269 519 0.0 6 513 6 270 225 0.0 6 222 2 1 271 244 0.0 6 239 5 272 770 0.0 6 760 10 273 467 0.0 6 457 9 1 274 755 0.0 6 744 10 1 275 407 0.0 6 400 6 1 276 871 0.0 6 842 29 277 2055 0.0 6 2018 37 278 916 0.0 6 890 23 1 1 1 279 11524 0.0 6 11182 337 5 280 137 0.0 6 118 19 281 20 0.0 6 17 2 1 282 2 0.0 6 2 283 3 0.0 6 3 291 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 542 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 1 0.0 6 1 61 1 0.0 6 1 62 1 0.0 6 1 63 58 0.0 6 56 2 64 1 0.0 6 1 72 4 0.0 6 4 73 8 0.0 6 8 74 8 0.0 6 8 75 10 0.0 6 10 76 3 0.0 6 2 1 77 10 0.0 6 10 78 13 0.0 6 13 79 9 0.0 6 9 80 7 0.0 6 7 81 15 0.0 6 15 82 14 0.0 6 14 83 5 0.0 6 5 84 7 0.0 6 7 85 6 0.0 6 6 86 8 0.0 6 8 87 4 0.0 6 4 88 2 0.0 6 2 89 4 0.0 6 4 90 9 0.0 6 9 91 8 0.0 6 8 92 11 0.0 6 11 93 11 0.0 6 11 94 6 0.0 6 6 95 5 0.0 6 5 96 13 0.0 6 13 97 6 0.0 6 6 98 3 0.0 6 3 99 5 0.0 6 5 100 9 0.0 6 9 101 6 0.0 6 6 102 4 0.0 6 4 103 5 0.0 6 5 104 2 0.0 6 2 105 4 0.0 6 4 106 4 0.0 6 4 107 3 0.0 6 3 108 3 0.0 6 3 109 3 0.0 6 3 110 2 0.0 6 2 111 4 0.0 6 4 112 10 0.0 6 10 113 7 0.0 6 7 114 4 0.0 6 4 115 4 0.0 6 4 116 7 0.0 6 7 117 3 0.0 6 3 118 4 0.0 6 4 119 5 0.0 6 5 120 1 0.0 6 1 122 1 0.0 6 1 123 3 0.0 6 3 124 3 0.0 6 3 125 2 0.0 6 2 126 3 0.0 6 3 127 2 0.0 6 2 128 2 0.0 6 2 129 3 0.0 6 3 130 1 0.0 6 1 131 3 0.0 6 3 132 1 0.0 6 1 133 1 0.0 6 1 134 3 0.0 6 3 135 2 0.0 6 2 136 1 0.0 6 1 137 4 0.0 6 4 138 1 0.0 6 1 139 2 0.0 6 2 140 3 0.0 6 3 141 1 0.0 6 1 142 1 0.0 6 1 143 4 0.0 6 4 144 2 0.0 6 2 146 2 0.0 6 2 147 3 0.0 6 3 150 2 0.0 6 2 151 2 0.0 6 2 152 2 0.0 6 2 153 2 0.0 6 2 155 2 0.0 6 2 157 3 0.0 6 3 159 2 0.0 6 2 160 1 0.0 6 1 161 4 0.0 6 4 162 2 0.0 6 2 163 2 0.0 6 2 164 2 0.0 6 2 165 1 0.0 6 1 167 2 0.0 6 2 168 1 0.0 6 1 170 2 0.0 6 2 171 2 0.0 6 2 172 2 0.0 6 2 173 1 0.0 6 1 174 2 0.0 6 2 175 1 0.0 6 1 177 2 0.0 6 2 178 1 0.0 6 1 179 2 0.0 6 2 181 3 0.0 6 3 183 2 0.0 6 2 184 2 0.0 6 2 185 4 0.0 6 4 186 2 0.0 6 2 187 4 0.0 6 4 188 3 0.0 6 3 191 3 0.0 6 3 192 3 0.0 6 3 193 1 0.0 6 1 194 1 0.0 6 1 195 1 0.0 6 1 197 1 0.0 6 1 198 3 0.0 6 3 200 1 0.0 6 1 202 1 0.0 6 1 204 1 0.0 6 1 205 1 0.0 6 1 206 2 0.0 6 2 207 1 0.0 6 1 208 2 0.0 6 2 209 1 0.0 6 1 210 1 0.0 6 1 212 1 0.0 6 1 213 1 0.0 6 1 215 2 0.0 6 2 216 1 0.0 6 1 217 1 0.0 6 1 218 1 0.0 6 1 219 1 0.0 6 1 222 1 0.0 6 1 225 1 0.0 6 1 226 1 0.0 6 1 227 2 0.0 6 2 234 2 0.0 6 2 236 1 0.0 6 1 239 1 0.0 6 1 240 1 0.0 6 1 241 1 0.0 6 1 243 2 0.0 6 2 247 1 0.0 6 1 249 2 0.0 6 2 261 2 0.0 6 2 265 1 0.0 6 1 278 1 0.0 6 1 286 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 51696 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 203.9 1 1 5 1 51.0 1 1 10 2 0.0 3 2 11 1 0.0 3 0 1 12 4 0.0 3 3 1 13 7 0.0 3 4 3 14 4 0.0 4 3 1 16 9 0.0 4 8 1 17 15 0.0 5 11 4 18 5 0.0 5 5 19 9 0.0 5 8 1 20 46 0.0 6 46 21 47 0.0 6 42 5 22 20 0.0 6 20 23 19 0.0 6 18 1 24 24 0.0 6 21 3 25 20 0.0 6 17 3 26 8 0.0 6 8 27 17 0.0 6 16 1 28 15 0.0 6 13 2 29 8 0.0 6 7 1 30 12 0.0 6 11 0 0 0 1 31 8 0.0 6 7 1 32 7 0.0 6 6 0 0 0 1 33 10 0.0 6 8 2 34 12 0.0 6 10 2 35 9 0.0 6 9 36 4 0.0 6 3 1 37 9 0.0 6 9 38 10 0.0 6 9 1 39 6 0.0 6 5 1 40 6 0.0 6 5 1 41 7 0.0 6 7 42 11 0.0 6 9 1 1 43 3 0.0 6 2 1 44 11 0.0 6 10 1 45 12 0.0 6 10 2 46 11 0.0 6 9 2 47 12 0.0 6 10 2 48 12 0.0 6 12 49 16 0.0 6 15 1 50 16 0.0 6 14 2 51 8 0.0 6 8 52 9 0.0 6 9 53 20 0.0 6 18 2 54 17 0.0 6 15 2 55 19 0.0 6 16 3 56 13 0.0 6 11 2 57 11 0.0 6 10 1 58 13 0.0 6 12 1 59 12 0.0 6 7 4 0 0 1 60 21 0.0 6 19 1 0 0 0 0 1 61 11 0.0 6 11 62 14 0.0 6 12 2 63 16 0.0 6 14 2 64 10 0.0 6 9 1 65 18 0.0 6 16 1 0 0 1 66 16 0.0 6 16 67 105 0.0 6 98 7 68 31 0.0 6 30 1 69 49 0.0 6 48 1 70 29 0.0 6 26 3 71 25 0.0 6 25 72 22 0.0 6 19 3 73 8 0.0 6 6 2 74 12 0.0 6 11 1 75 21 0.0 6 18 2 0 0 1 76 30 0.0 6 27 2 0 0 1 77 16 0.0 6 14 1 0 1 78 20 0.0 6 19 0 1 79 15 0.0 6 13 2 80 30 0.0 6 27 3 81 30 0.0 6 27 3 82 32 0.0 6 28 4 83 40 0.0 6 39 1 84 35 0.0 6 31 3 0 1 85 20 0.0 6 18 1 0 0 0 1 86 23 0.0 6 22 1 87 38 0.0 6 36 2 88 21 0.0 6 20 0 0 1 89 31 0.0 6 28 2 1 90 33 0.0 6 32 1 91 45 0.0 6 43 2 92 21 0.0 6 20 1 93 43 0.0 6 40 3 94 51 0.0 6 49 2 95 33 0.0 6 32 1 96 42 0.0 6 41 0 1 97 39 0.0 6 36 3 98 50 0.0 6 49 1 99 45 0.0 6 44 0 0 1 100 46 0.0 6 42 2 1 0 0 0 1 101 39 0.0 6 38 1 102 56 0.0 6 55 1 103 66 0.0 6 63 3 104 73 0.0 6 70 3 105 52 0.0 6 48 4 106 40 0.0 6 40 107 51 0.0 6 48 3 108 39 0.0 6 39 109 59 0.0 6 56 3 110 75 0.0 6 72 2 0 0 1 111 82 0.0 6 79 2 0 0 1 112 57 0.0 6 56 1 113 58 0.0 6 55 3 114 88 0.0 6 83 4 0 0 1 115 70 0.0 6 69 1 116 74 0.0 6 71 2 1 117 63 0.0 6 57 6 118 65 0.0 6 64 1 119 87 0.0 6 87 120 86 0.0 6 85 1 121 60 0.0 6 58 2 122 85 0.0 6 83 2 123 88 0.0 6 86 1 0 0 0 1 124 133 0.0 6 132 1 125 109 0.0 6 106 3 126 117 0.0 6 112 5 127 117 0.0 6 113 4 128 127 0.0 6 126 1 129 107 0.0 6 104 3 130 119 0.0 6 117 2 131 179 0.0 6 175 3 0 1 132 209 0.0 6 202 6 0 0 0 1 133 202 0.0 6 197 3 2 134 267 0.0 6 266 0 0 1 135 280 0.0 6 278 2 136 186 0.0 6 185 1 137 222 0.0 6 219 2 1 138 163 0.0 6 158 5 139 217 0.0 6 211 5 0 0 1 140 346 0.0 6 342 3 0 1 141 430 0.0 6 426 4 142 544 0.0 6 536 6 1 1 143 515 0.0 6 506 8 1 144 548 0.0 6 543 5 145 487 0.0 6 479 7 0 0 0 1 146 575 0.0 6 568 6 1 147 556 0.0 6 553 3 148 548 0.0 6 544 3 1 149 731 0.0 6 719 11 0 1 150 1038 0.0 6 1016 21 1 151 577 0.0 6 568 7 1 0 1 152 795 0.0 6 777 16 1 0 1 153 633 0.0 6 622 10 1 154 580 0.0 6 571 9 155 576 0.0 6 572 4 156 418 0.0 6 412 5 0 0 1 157 639 0.0 6 630 9 158 648 0.0 6 640 8 159 465 0.0 6 460 5 160 541 0.0 6 533 6 0 0 2 161 481 0.0 6 478 2 0 0 0 0 1 162 331 0.0 6 328 3 163 448 0.0 6 438 9 1 164 413 0.0 6 408 5 165 471 0.0 6 461 10 166 475 0.0 6 468 6 1 167 414 0.0 6 405 8 0 1 168 587 0.0 6 580 6 1 169 378 0.0 6 373 5 170 309 0.0 6 304 5 171 470 0.0 6 459 10 1 172 401 0.0 6 394 6 1 173 423 0.0 6 410 12 0 0 1 174 577 0.0 6 569 8 175 669 0.0 6 658 11 176 594 0.0 6 575 19 177 514 0.0 6 499 14 0 0 1 178 447 0.0 6 435 12 179 437 0.0 6 421 14 2 180 379 0.0 6 367 11 1 181 438 0.0 6 429 9 182 185 0.0 6 181 4 183 524 0.0 6 507 17 184 450 0.0 6 442 7 0 0 1 185 541 0.0 6 530 9 0 0 2 186 914 0.0 6 895 16 2 1 187 712 0.0 6 691 20 0 1 188 701 0.0 6 686 14 0 0 1 189 564 0.0 6 542 21 0 1 190 894 0.0 6 858 33 2 1 191 1009 0.0 6 981 27 1 192 536 0.0 6 523 11 1 0 1 193 371 0.0 6 359 9 1 1 1 194 626 0.0 6 609 16 1 195 815 0.0 6 787 26 2 196 1447 0.0 6 1408 38 0 0 1 197 551 0.0 6 521 28 2 198 999 0.0 6 965 32 2 199 1441 0.0 6 1399 41 1 200 578 0.0 6 560 18 201 846 0.0 6 820 26 202 375 0.0 6 369 6 203 350 0.0 6 337 13 204 686 0.0 6 676 9 0 1 205 194 0.0 6 190 4 206 212 0.0 6 209 3 207 117 0.0 6 114 3 208 249 0.0 6 243 6 209 229 0.0 6 222 7 210 226 0.0 6 218 8 211 168 0.0 6 163 5 212 360 0.0 6 349 11 213 209 0.0 6 201 8 214 852 0.0 6 834 15 0 0 1 1 1 215 373 0.0 6 364 8 1 216 289 0.0 6 280 9 217 322 0.0 6 313 8 0 0 1 218 185 0.0 6 178 7 219 305 0.0 6 292 13 220 48 0.0 6 48 221 219 0.0 6 213 6 222 276 0.0 6 269 5 0 0 1 0 1 223 349 0.0 6 342 6 1 224 146 0.0 6 139 7 225 209 0.0 6 206 3 226 140 0.0 6 137 3 227 152 0.0 6 151 1 228 89 0.0 6 85 3 0 0 1 229 184 0.0 6 182 2 230 104 0.0 6 101 3 231 155 0.0 6 151 4 232 200 0.0 6 194 6 233 122 0.0 6 119 3 234 90 0.0 6 86 4 235 66 0.0 6 63 2 1 236 8 0.0 6 8 237 13 0.0 6 12 1 238 27 0.0 6 25 2 239 25 0.0 6 24 1 240 71 0.0 6 69 2 241 97 0.0 6 92 5 242 124 0.0 6 118 5 1 243 97 0.0 6 94 3 244 61 0.0 6 58 3 245 36 0.0 6 36 246 62 0.0 6 60 2 247 15 0.0 6 15 248 42 0.0 6 41 1 249 10 0.0 6 10 250 14 0.0 6 14 251 29 0.0 6 29 252 7 0.0 6 7 253 2 0.0 6 2 255 2 0.0 6 2 277 1 0.0 6 1 278 13 0.0 6 13 279 28 0.0 6 27 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 487 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 66 1 0.0 6 1 68 1 0.0 6 1 71 2 0.0 6 2 72 3 0.0 6 3 73 2 0.0 6 2 74 12 0.0 6 12 75 8 0.0 6 8 76 8 0.0 6 8 77 11 0.0 6 11 78 7 0.0 6 7 79 10 0.0 6 9 1 80 13 0.0 6 13 81 10 0.0 6 10 82 10 0.0 6 10 83 13 0.0 6 13 84 8 0.0 6 8 85 9 0.0 6 9 86 8 0.0 6 8 87 7 0.0 6 6 0 0 0 1 88 6 0.0 6 6 89 11 0.0 6 11 90 3 0.0 6 3 91 11 0.0 6 11 92 13 0.0 6 13 93 6 0.0 6 6 94 12 0.0 6 12 95 4 0.0 6 4 96 20 0.0 6 20 97 8 0.0 6 8 98 5 0.0 6 5 99 9 0.0 6 9 100 8 0.0 6 8 101 5 0.0 6 5 102 1 0.0 6 1 103 4 0.0 6 4 104 7 0.0 6 7 105 4 0.0 6 4 106 4 0.0 6 4 107 3 0.0 6 3 108 4 0.0 6 4 109 4 0.0 6 4 110 7 0.0 6 7 111 3 0.0 6 3 112 5 0.0 6 5 113 5 0.0 6 5 114 3 0.0 6 3 115 5 0.0 6 5 116 5 0.0 6 4 1 117 4 0.0 6 4 118 4 0.0 6 4 119 2 0.0 6 2 120 5 0.0 6 5 121 3 0.0 6 3 122 6 0.0 6 6 123 4 0.0 6 4 124 2 0.0 6 2 125 3 0.0 6 3 126 3 0.0 6 3 127 3 0.0 6 3 128 1 0.0 6 1 129 3 0.0 6 3 130 2 0.0 6 2 131 1 0.0 6 1 133 2 0.0 6 2 134 5 0.0 6 5 135 5 0.0 6 5 136 2 0.0 6 2 137 3 0.0 6 3 138 3 0.0 6 3 139 2 0.0 6 2 141 3 0.0 6 3 142 2 0.0 6 2 143 3 0.0 6 3 144 5 0.0 6 5 145 1 0.0 6 1 146 3 0.0 6 3 147 4 0.0 6 4 148 2 0.0 6 2 149 3 0.0 6 2 0 0 0 1 150 4 0.0 6 4 151 2 0.0 6 2 152 2 0.0 6 2 153 4 0.0 6 4 154 1 0.0 6 1 155 2 0.0 6 2 156 3 0.0 6 3 157 1 0.0 6 1 160 1 0.0 6 1 161 1 0.0 6 1 162 2 0.0 6 2 163 1 0.0 6 1 164 2 0.0 6 2 165 4 0.0 6 4 166 2 0.0 6 2 167 1 0.0 6 1 168 1 0.0 6 1 169 2 0.0 6 2 170 3 0.0 6 3 171 1 0.0 6 1 172 1 0.0 6 1 176 2 0.0 6 2 177 1 0.0 6 1 179 1 0.0 6 1 180 1 0.0 6 1 181 2 0.0 6 2 183 2 0.0 6 2 185 1 0.0 6 1 189 1 0.0 6 1 192 1 0.0 6 1 194 1 0.0 6 1 197 1 0.0 6 1 200 1 0.0 6 1 201 2 0.0 6 2 208 1 0.0 6 1 213 2 0.0 6 2 219 1 0.0 6 1 221 1 0.0 6 1 225 1 0.0 6 1 226 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_11_155_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_11_382_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_11_155_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_11_382_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.04 s (109 µs/read; 0.55 M reads/minute). === Summary === Total read pairs processed: 36,932 Read 1 with adapter: 36,924 (100.0%) Read 2 with adapter: 36,926 (100.0%) == Read fate breakdown == Pairs that were too short: 11 (0.0%) Pairs discarded as untrimmed: 14 (0.0%) Pairs written (passing filters): 36,907 (99.9%) Total basepairs processed: 15,604,028 bp Read 1: 8,353,373 bp Read 2: 7,250,655 bp Total written (filtered): 2,380,637 bp (15.3%) Read 1: 1,254,674 bp Read 2: 1,125,963 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 35639 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 2 144.3 1 0 2 6 2 9.0 1 0 0 2 7 3 2.3 2 1 0 2 14 2 0.0 4 2 17 2 0.0 5 2 19 3 0.0 5 3 20 31 0.0 6 29 2 21 24 0.0 6 21 2 1 22 14 0.0 6 14 23 9 0.0 6 9 24 10 0.0 6 8 1 0 1 25 11 0.0 6 11 26 4 0.0 6 2 2 27 6 0.0 6 5 1 28 5 0.0 6 4 1 29 5 0.0 6 5 30 5 0.0 6 5 31 5 0.0 6 5 32 3 0.0 6 3 33 1 0.0 6 0 0 1 34 1 0.0 6 1 35 3 0.0 6 3 36 6 0.0 6 6 37 4 0.0 6 4 38 2 0.0 6 2 40 7 0.0 6 6 1 41 2 0.0 6 2 42 3 0.0 6 3 43 3 0.0 6 3 44 6 0.0 6 6 45 4 0.0 6 4 46 1 0.0 6 1 47 7 0.0 6 7 48 2 0.0 6 2 49 3 0.0 6 3 50 5 0.0 6 4 0 0 1 51 9 0.0 6 8 1 52 3 0.0 6 3 53 3 0.0 6 2 1 54 3 0.0 6 2 0 0 0 0 0 1 55 2 0.0 6 2 56 5 0.0 6 5 57 1 0.0 6 1 59 4 0.0 6 4 61 4 0.0 6 3 0 0 0 0 0 1 62 1 0.0 6 1 63 2 0.0 6 2 64 4 0.0 6 4 65 4 0.0 6 4 66 1 0.0 6 1 67 5 0.0 6 4 1 68 3 0.0 6 3 69 3 0.0 6 3 70 4 0.0 6 4 71 10 0.0 6 9 0 0 1 72 4 0.0 6 3 0 0 0 1 73 6 0.0 6 5 1 74 7 0.0 6 7 75 7 0.0 6 6 1 76 2 0.0 6 2 77 3 0.0 6 3 78 2 0.0 6 2 79 4 0.0 6 4 80 4 0.0 6 4 81 4 0.0 6 4 82 9 0.0 6 9 83 5 0.0 6 5 84 2 0.0 6 2 85 3 0.0 6 3 86 4 0.0 6 3 0 1 87 4 0.0 6 4 88 2 0.0 6 2 89 7 0.0 6 7 90 2 0.0 6 2 91 5 0.0 6 4 0 1 92 2 0.0 6 2 93 1 0.0 6 1 94 5 0.0 6 5 95 8 0.0 6 8 96 6 0.0 6 6 97 7 0.0 6 7 98 2 0.0 6 2 99 5 0.0 6 5 100 6 0.0 6 5 1 101 10 0.0 6 10 102 5 0.0 6 5 103 10 0.0 6 10 104 13 0.0 6 13 105 9 0.0 6 8 0 0 0 0 1 106 7 0.0 6 7 107 8 0.0 6 8 108 7 0.0 6 7 109 10 0.0 6 10 110 12 0.0 6 12 111 3 0.0 6 3 112 7 0.0 6 7 113 16 0.0 6 16 114 9 0.0 6 8 1 115 12 0.0 6 11 1 116 6 0.0 6 6 117 9 0.0 6 9 118 8 0.0 6 8 119 17 0.0 6 17 120 14 0.0 6 12 2 121 13 0.0 6 13 122 13 0.0 6 13 123 21 0.0 6 21 124 12 0.0 6 12 125 15 0.0 6 15 126 26 0.0 6 26 127 17 0.0 6 17 128 29 0.0 6 29 129 24 0.0 6 24 130 24 0.0 6 24 131 14 0.0 6 13 1 132 25 0.0 6 25 133 32 0.0 6 32 134 16 0.0 6 16 135 18 0.0 6 17 1 136 23 0.0 6 23 137 24 0.0 6 24 138 40 0.0 6 40 139 58 0.0 6 58 140 282 0.0 6 281 1 141 702 0.0 6 701 1 142 928 0.0 6 924 3 0 0 1 143 561 0.0 6 560 1 144 305 0.0 6 305 145 94 0.0 6 93 1 146 655 0.0 6 654 1 147 422 0.0 6 422 148 73 0.0 6 73 149 32 0.0 6 32 150 46 0.0 6 45 1 151 42 0.0 6 42 152 47 0.0 6 46 1 153 52 0.0 6 52 154 106 0.0 6 106 155 109 0.0 6 109 156 591 0.0 6 588 3 157 362 0.0 6 362 158 145 0.0 6 145 159 590 0.0 6 590 160 205 0.0 6 205 161 633 0.0 6 633 162 683 0.0 6 683 163 161 0.0 6 160 0 0 1 164 186 0.0 6 185 1 165 777 0.0 6 774 2 0 1 166 810 0.0 6 809 1 167 154 0.0 6 154 168 121 0.0 6 121 169 797 0.0 6 795 2 170 124 0.0 6 123 0 1 171 48 0.0 6 47 1 172 61 0.0 6 61 173 52 0.0 6 52 174 118 0.0 6 117 1 175 729 0.0 6 727 2 176 129 0.0 6 129 177 64 0.0 6 64 178 59 0.0 6 58 0 0 0 1 179 101 0.0 6 100 1 180 551 0.0 6 549 2 181 113 0.0 6 113 182 79 0.0 6 77 0 1 1 183 72 0.0 6 72 184 130 0.0 6 130 185 251 0.0 6 249 1 0 0 0 1 186 1380 0.0 6 1377 2 0 1 187 210 0.0 6 210 188 66 0.0 6 65 1 189 71 0.0 6 69 1 1 190 81 0.0 6 79 2 191 75 0.0 6 74 0 1 192 143 0.0 6 143 193 184 0.0 6 182 2 194 656 0.0 6 653 3 195 536 0.0 6 536 196 415 0.0 6 415 197 1602 0.0 6 1598 2 2 198 1310 0.0 6 1309 1 199 179 0.0 6 177 2 200 86 0.0 6 85 0 1 201 155 0.0 6 154 1 202 105 0.0 6 104 1 203 115 0.0 6 115 204 149 0.0 6 146 2 1 205 177 0.0 6 176 1 206 273 0.0 6 271 2 207 341 0.0 6 341 208 579 0.0 6 578 1 209 344 0.0 6 341 3 210 478 0.0 6 476 1 0 0 0 1 211 253 0.0 6 253 212 398 0.0 6 397 1 213 970 0.0 6 968 2 214 91 0.0 6 91 215 76 0.0 6 75 1 216 70 0.0 6 70 217 48 0.0 6 48 218 103 0.0 6 102 1 219 74 0.0 6 73 0 0 0 1 220 105 0.0 6 104 1 221 125 0.0 6 125 222 129 0.0 6 128 1 223 117 0.0 6 116 1 224 124 0.0 6 123 1 225 250 0.0 6 250 226 141 0.0 6 141 227 146 0.0 6 146 228 178 0.0 6 177 1 229 125 0.0 6 124 1 230 230 0.0 6 228 2 231 219 0.0 6 218 1 232 161 0.0 6 161 233 247 0.0 6 244 3 234 341 0.0 6 341 235 141 0.0 6 141 236 194 0.0 6 194 237 218 0.0 6 215 2 0 0 1 238 191 0.0 6 187 3 0 1 239 247 0.0 6 247 240 274 0.0 6 274 241 341 0.0 6 341 242 431 0.0 6 431 243 592 0.0 6 591 1 244 229 0.0 6 228 0 0 1 245 339 0.0 6 338 0 1 246 771 0.0 6 769 2 247 125 0.0 6 124 1 248 42 0.0 6 42 249 50 0.0 6 50 250 46 0.0 6 45 1 251 92 0.0 6 92 252 83 0.0 6 83 253 185 0.0 6 184 1 254 23 0.0 6 23 255 25 0.0 6 25 256 25 0.0 6 25 257 6 0.0 6 5 1 258 14 0.0 6 14 259 19 0.0 6 19 260 15 0.0 6 13 2 261 19 0.0 6 18 1 262 14 0.0 6 14 263 21 0.0 6 20 1 264 25 0.0 6 23 1 0 1 265 21 0.0 6 20 1 266 11 0.0 6 11 267 14 0.0 6 13 1 268 20 0.0 6 19 1 269 29 0.0 6 29 270 19 0.0 6 18 1 271 9 0.0 6 9 272 17 0.0 6 17 273 27 0.0 6 27 274 34 0.0 6 34 275 25 0.0 6 24 1 276 37 0.0 6 36 1 277 42 0.0 6 41 0 0 0 0 0 1 278 202 0.0 6 199 2 0 1 279 1497 0.0 6 1490 5 0 1 1 280 12 0.0 6 11 1 282 1 0.0 6 1 283 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1285 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 68 1 0.0 6 0 0 0 0 0 1 71 1 0.0 6 1 72 14 0.0 6 14 73 24 0.0 6 24 74 39 0.0 6 39 75 16 0.0 6 16 76 13 0.0 6 13 77 4 0.0 6 4 78 23 0.0 6 23 79 12 0.0 6 12 80 2 0.0 6 1 0 0 0 0 0 1 85 2 0.0 6 2 86 5 0.0 6 5 87 8 0.0 6 8 88 19 0.0 6 19 89 11 0.0 6 11 90 7 0.0 6 7 91 19 0.0 6 19 92 6 0.0 6 6 93 26 0.0 6 26 94 30 0.0 6 30 96 4 0.0 6 4 97 32 0.0 6 32 98 34 0.0 6 34 99 3 0.0 6 3 100 8 0.0 6 8 101 31 0.0 6 31 105 2 0.0 6 2 106 11 0.0 6 11 107 32 0.0 6 32 111 10 0.0 6 10 112 19 0.0 6 19 113 1 0.0 6 1 114 1 0.0 6 1 116 1 0.0 6 1 117 18 0.0 6 18 118 63 0.0 6 63 119 4 0.0 6 4 121 1 0.0 6 1 123 1 0.0 6 1 125 9 0.0 6 9 126 31 0.0 6 30 1 127 15 0.0 6 15 128 26 0.0 6 26 129 83 0.0 6 83 130 64 0.0 6 64 131 3 0.0 6 3 139 10 0.0 6 10 140 28 0.0 6 28 141 1 0.0 6 1 154 1 0.0 6 1 155 1 0.0 6 1 164 1 0.0 6 1 165 7 0.0 6 7 166 16 0.0 6 16 167 1 0.0 6 1 170 2 0.0 6 2 172 3 0.0 6 3 173 4 0.0 6 4 174 52 0.0 6 52 175 78 0.0 6 78 176 7 0.0 6 6 1 177 42 0.0 6 42 178 140 0.0 6 139 1 179 7 0.0 6 7 184 1 0.0 6 0 0 0 0 0 0 1 208 1 0.0 6 1 215 1 0.0 6 1 216 2 0.0 6 2 217 25 0.0 6 25 218 57 0.0 6 57 219 6 0.0 6 6 275 1 0.0 6 1 276 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 35514 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 11 1 0.0 3 1 12 1 0.0 3 1 13 3 0.0 3 3 14 2 0.0 4 2 15 2 0.0 4 1 1 16 1 0.0 4 1 17 4 0.0 5 4 18 2 0.0 5 2 19 7 0.0 5 7 20 18 0.0 6 17 1 21 12 0.0 6 11 1 22 8 0.0 6 8 23 5 0.0 6 5 24 10 0.0 6 8 2 25 5 0.0 6 5 26 8 0.0 6 8 27 5 0.0 6 4 1 28 13 0.0 6 12 1 29 4 0.0 6 4 30 2 0.0 6 2 31 2 0.0 6 1 1 32 1 0.0 6 1 33 2 0.0 6 2 34 4 0.0 6 4 35 2 0.0 6 2 36 5 0.0 6 5 37 2 0.0 6 2 38 2 0.0 6 2 39 5 0.0 6 5 40 4 0.0 6 4 41 5 0.0 6 5 43 5 0.0 6 5 44 4 0.0 6 4 45 4 0.0 6 3 0 0 1 46 6 0.0 6 5 0 0 1 47 7 0.0 6 5 2 48 7 0.0 6 7 49 3 0.0 6 3 50 6 0.0 6 5 1 51 9 0.0 6 9 52 5 0.0 6 5 53 5 0.0 6 4 0 0 0 0 1 54 1 0.0 6 1 55 6 0.0 6 6 56 4 0.0 6 3 1 57 6 0.0 6 5 1 58 4 0.0 6 4 59 2 0.0 6 2 60 2 0.0 6 2 61 6 0.0 6 5 1 62 6 0.0 6 6 63 3 0.0 6 3 64 6 0.0 6 4 1 0 0 0 0 1 65 7 0.0 6 6 1 66 11 0.0 6 10 1 67 7 0.0 6 7 68 9 0.0 6 9 69 22 0.0 6 22 70 57 0.0 6 54 1 1 1 71 18 0.0 6 16 2 72 24 0.0 6 22 2 73 11 0.0 6 10 1 74 26 0.0 6 23 3 75 10 0.0 6 10 76 9 0.0 6 8 0 0 1 77 20 0.0 6 20 78 10 0.0 6 9 1 79 16 0.0 6 15 1 80 6 0.0 6 6 81 14 0.0 6 14 82 13 0.0 6 12 1 83 13 0.0 6 13 84 17 0.0 6 17 85 18 0.0 6 16 0 2 86 30 0.0 6 29 0 0 0 0 1 87 15 0.0 6 14 1 88 9 0.0 6 8 0 1 89 8 0.0 6 7 0 1 90 14 0.0 6 14 91 14 0.0 6 13 1 92 10 0.0 6 10 93 6 0.0 6 6 94 26 0.0 6 21 4 0 1 95 14 0.0 6 14 96 24 0.0 6 23 1 97 16 0.0 6 16 98 17 0.0 6 16 1 99 21 0.0 6 21 100 16 0.0 6 15 1 101 20 0.0 6 19 1 102 13 0.0 6 12 1 103 9 0.0 6 9 104 15 0.0 6 15 105 22 0.0 6 21 1 106 28 0.0 6 27 1 107 38 0.0 6 37 0 1 108 31 0.0 6 28 3 109 29 0.0 6 29 110 14 0.0 6 14 111 27 0.0 6 27 112 29 0.0 6 28 1 113 32 0.0 6 32 114 43 0.0 6 43 115 43 0.0 6 43 116 39 0.0 6 39 117 44 0.0 6 43 1 118 54 0.0 6 52 2 119 34 0.0 6 34 120 56 0.0 6 54 1 1 121 55 0.0 6 55 122 51 0.0 6 51 123 47 0.0 6 47 124 62 0.0 6 61 1 125 56 0.0 6 56 126 66 0.0 6 66 127 69 0.0 6 69 128 66 0.0 6 64 2 129 87 0.0 6 86 0 1 130 70 0.0 6 70 131 78 0.0 6 77 1 132 90 0.0 6 86 3 0 1 133 106 0.0 6 105 1 134 85 0.0 6 84 1 135 119 0.0 6 115 3 0 1 136 180 0.0 6 176 4 137 212 0.0 6 210 1 0 1 138 239 0.0 6 238 1 139 275 0.0 6 273 2 140 451 0.0 6 447 4 141 705 0.0 6 700 4 0 0 1 142 890 0.0 6 885 5 143 561 0.0 6 560 1 144 472 0.0 6 470 2 145 395 0.0 6 393 2 146 720 0.0 6 718 2 147 472 0.0 6 471 1 148 272 0.0 6 268 3 1 149 263 0.0 6 263 150 200 0.0 6 198 1 1 151 205 0.0 6 204 1 152 342 0.0 6 339 2 0 0 1 153 404 0.0 6 404 154 445 0.0 6 443 2 155 491 0.0 6 489 2 156 786 0.0 6 780 5 0 0 1 157 507 0.0 6 507 158 481 0.0 6 478 1 1 0 1 159 613 0.0 6 610 3 160 637 0.0 6 636 0 0 0 1 161 681 0.0 6 681 162 596 0.0 6 591 2 0 1 1 1 163 492 0.0 6 488 4 164 504 0.0 6 502 2 165 637 0.0 6 636 0 0 0 1 166 663 0.0 6 661 1 0 0 1 167 456 0.0 6 453 2 0 0 1 168 437 0.0 6 434 0 1 0 1 1 169 726 0.0 6 725 1 170 356 0.0 6 355 0 0 0 1 171 516 0.0 6 514 1 0 1 172 353 0.0 6 352 1 173 333 0.0 6 330 3 174 580 0.0 6 578 2 175 666 0.0 6 662 3 0 0 1 176 340 0.0 6 337 3 177 477 0.0 6 474 3 178 494 0.0 6 493 1 179 511 0.0 6 506 3 0 0 2 180 571 0.0 6 568 3 181 517 0.0 6 513 4 182 303 0.0 6 303 183 306 0.0 6 302 3 0 1 184 391 0.0 6 386 1 0 0 4 185 415 0.0 6 413 1 0 0 1 186 845 0.0 6 842 2 0 1 187 615 0.0 6 614 1 188 553 0.0 6 551 2 189 470 0.0 6 469 1 190 420 0.0 6 419 1 191 534 0.0 6 533 1 192 636 0.0 6 636 193 329 0.0 6 327 2 194 335 0.0 6 334 1 195 415 0.0 6 414 1 196 182 0.0 6 179 3 197 251 0.0 6 250 1 198 198 0.0 6 197 1 199 185 0.0 6 184 1 200 115 0.0 6 115 201 184 0.0 6 183 1 202 179 0.0 6 177 2 203 184 0.0 6 184 204 160 0.0 6 160 205 148 0.0 6 148 206 162 0.0 6 162 207 98 0.0 6 95 2 1 208 148 0.0 6 148 209 140 0.0 6 140 210 148 0.0 6 147 0 1 211 160 0.0 6 160 212 108 0.0 6 108 213 93 0.0 6 93 214 111 0.0 6 111 215 71 0.0 6 71 216 100 0.0 6 100 217 64 0.0 6 64 218 61 0.0 6 60 0 0 0 1 219 82 0.0 6 82 220 101 0.0 6 100 0 0 0 1 221 47 0.0 6 47 222 39 0.0 6 39 223 32 0.0 6 32 224 34 0.0 6 34 225 37 0.0 6 37 226 62 0.0 6 62 227 89 0.0 6 89 228 57 0.0 6 57 229 33 0.0 6 33 230 46 0.0 6 46 231 35 0.0 6 35 232 36 0.0 6 36 233 29 0.0 6 28 0 0 0 0 1 234 27 0.0 6 27 235 23 0.0 6 23 236 12 0.0 6 12 237 9 0.0 6 9 238 3 0.0 6 3 241 8 0.0 6 8 242 8 0.0 6 8 243 1 0.0 6 1 244 27 0.0 6 27 245 6 0.0 6 6 246 8 0.0 6 8 247 1 0.0 6 1 248 1 0.0 6 1 251 1 0.0 6 1 252 4 0.0 6 4 253 2 0.0 6 2 254 1 0.0 6 1 257 1 0.0 6 1 278 1 0.0 6 1 281 8 0.0 6 8 282 103 0.0 6 103 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1412 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 68 2 0.0 6 2 69 23 0.0 6 23 70 29 0.0 6 28 1 71 44 0.0 6 44 72 32 0.0 6 32 73 9 0.0 6 9 74 3 0.0 6 3 75 28 0.0 6 28 76 20 0.0 6 20 77 1 0.0 6 1 82 1 0.0 6 1 83 3 0.0 6 3 84 6 0.0 6 6 85 30 0.0 6 30 86 14 0.0 6 14 87 5 0.0 6 5 88 25 0.0 6 25 89 3 0.0 6 3 90 42 0.0 6 42 91 27 0.0 6 27 92 1 0.0 6 1 93 11 0.0 6 11 94 35 0.0 6 35 95 35 0.0 6 35 96 3 0.0 6 3 97 7 0.0 6 7 98 48 0.0 6 47 0 0 0 1 99 1 0.0 6 1 103 4 0.0 6 4 104 31 0.0 6 31 108 8 0.0 6 8 109 34 0.0 6 34 113 7 0.0 6 6 0 0 0 1 114 7 0.0 6 7 115 81 0.0 6 80 1 116 1 0.0 6 1 121 1 0.0 6 1 122 5 0.0 6 5 123 37 0.0 6 37 124 13 0.0 6 13 125 19 0.0 6 19 126 82 0.0 6 80 1 0 1 127 70 0.0 6 70 128 7 0.0 6 7 130 1 0.0 6 0 0 0 1 131 1 0.0 6 0 0 0 0 1 133 1 0.0 6 1 136 7 0.0 6 7 137 20 0.0 6 20 138 1 0.0 6 1 162 1 0.0 6 1 163 21 0.0 6 20 1 168 1 0.0 6 1 170 8 0.0 6 7 1 171 49 0.0 6 49 172 99 0.0 6 99 173 11 0.0 6 11 174 38 0.0 6 38 175 157 0.0 6 157 176 3 0.0 6 2 1 210 1 0.0 6 1 213 1 0.0 6 0 1 214 11 0.0 6 11 215 84 0.0 6 84 216 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_07_144_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_07_371_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_07_144_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_07_371_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_08_145_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_08_372_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_08_145_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_08_372_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_09_146_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_09_373_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_09_146_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_09_373_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_10_147_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_10_374_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_10_147_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_10_374_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_11_148_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_11_375_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_11_148_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_11_375_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_12_149_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_12_376_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_12_149_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_12_376_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_13_150_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_13-Rev_13_377_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_13_150_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_13-Rev_13_377_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_07_151_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_07_378_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_07_151_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_07_378_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_08_152_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_08_379_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_08_152_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_08_379_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_09_153_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_09_380_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_09_153_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_09_380_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_10_154_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_10_381_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_10_154_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_10_381_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_11_155_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_11_382_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_11_155_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_11_382_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_12_156_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_12_383_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_12_156_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_12_383_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.52 s (160 µs/read; 0.38 M reads/minute). === Summary === Total read pairs processed: 15,777 Read 1 with adapter: 15,777 (100.0%) Read 2 with adapter: 15,755 (99.9%) == Read fate breakdown == Pairs that were too short: 2 (0.0%) Pairs discarded as untrimmed: 22 (0.1%) Pairs written (passing filters): 15,753 (99.8%) Total basepairs processed: 7,856,647 bp Read 1: 4,466,003 bp Read 2: 3,390,644 bp Total written (filtered): 993,913 bp (12.7%) Read 1: 504,834 bp Read 2: 489,079 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 15762 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 3.9 1 0 0 1 9 1 0.1 2 0 1 14 1 0.0 4 1 19 1 0.0 5 1 20 3 0.0 6 3 21 2 0.0 6 1 1 22 1 0.0 6 1 24 3 0.0 6 3 25 2 0.0 6 2 26 1 0.0 6 1 29 1 0.0 6 1 31 1 0.0 6 1 33 1 0.0 6 0 1 34 1 0.0 6 1 35 1 0.0 6 1 38 2 0.0 6 1 0 1 44 1 0.0 6 1 47 1 0.0 6 1 50 1 0.0 6 1 52 1 0.0 6 1 53 1 0.0 6 1 55 1 0.0 6 1 63 1 0.0 6 1 65 1 0.0 6 1 66 1 0.0 6 1 67 1 0.0 6 1 72 2 0.0 6 2 74 1 0.0 6 1 76 1 0.0 6 1 78 1 0.0 6 1 80 1 0.0 6 0 1 82 1 0.0 6 1 84 1 0.0 6 1 85 1 0.0 6 1 86 2 0.0 6 2 89 3 0.0 6 2 1 92 1 0.0 6 1 97 2 0.0 6 2 98 1 0.0 6 1 100 2 0.0 6 2 101 4 0.0 6 4 102 1 0.0 6 1 103 3 0.0 6 3 104 1 0.0 6 0 1 105 1 0.0 6 1 106 1 0.0 6 0 1 108 4 0.0 6 4 109 3 0.0 6 3 111 1 0.0 6 1 112 1 0.0 6 1 113 1 0.0 6 0 0 1 114 1 0.0 6 1 115 1 0.0 6 0 1 117 1 0.0 6 1 118 1 0.0 6 1 119 2 0.0 6 1 1 123 6 0.0 6 6 124 1 0.0 6 1 126 3 0.0 6 3 128 5 0.0 6 5 129 2 0.0 6 2 130 4 0.0 6 4 131 1 0.0 6 1 132 1 0.0 6 1 133 3 0.0 6 3 134 6 0.0 6 6 135 5 0.0 6 5 136 6 0.0 6 4 2 137 9 0.0 6 9 138 3 0.0 6 3 139 3 0.0 6 3 140 5 0.0 6 5 141 13 0.0 6 13 142 26 0.0 6 26 143 45 0.0 6 45 144 21 0.0 6 21 145 13 0.0 6 13 146 25 0.0 6 24 1 147 20 0.0 6 20 148 35 0.0 6 35 149 12 0.0 6 12 150 10 0.0 6 10 151 9 0.0 6 9 152 13 0.0 6 13 153 12 0.0 6 12 154 17 0.0 6 16 1 155 11 0.0 6 10 1 156 6 0.0 6 6 157 12 0.0 6 12 158 21 0.0 6 20 1 159 8 0.0 6 8 160 9 0.0 6 9 161 9 0.0 6 9 162 22 0.0 6 22 163 9 0.0 6 9 164 9 0.0 6 9 165 8 0.0 6 8 166 11 0.0 6 11 167 3 0.0 6 3 168 4 0.0 6 3 1 169 6 0.0 6 5 1 170 26 0.0 6 26 171 12 0.0 6 12 172 23 0.0 6 23 173 11 0.0 6 11 174 18 0.0 6 18 175 12 0.0 6 12 176 15 0.0 6 15 177 7 0.0 6 7 178 10 0.0 6 10 179 19 0.0 6 19 180 22 0.0 6 20 2 181 23 0.0 6 23 182 31 0.0 6 31 183 12 0.0 6 12 184 8 0.0 6 7 1 185 26 0.0 6 26 186 37 0.0 6 37 187 24 0.0 6 23 1 188 48 0.0 6 47 1 189 19 0.0 6 18 1 190 42 0.0 6 40 2 191 40 0.0 6 38 2 192 31 0.0 6 30 1 193 60 0.0 6 59 1 194 54 0.0 6 51 2 1 195 81 0.0 6 79 2 196 68 0.0 6 63 5 197 85 0.0 6 85 198 87 0.0 6 85 2 199 48 0.0 6 46 2 200 111 0.0 6 109 2 201 77 0.0 6 75 2 202 77 0.0 6 76 1 203 54 0.0 6 54 204 89 0.0 6 86 3 205 77 0.0 6 74 3 206 128 0.0 6 126 1 1 207 79 0.0 6 76 3 208 75 0.0 6 74 1 209 24 0.0 6 24 210 42 0.0 6 40 2 211 47 0.0 6 46 1 212 38 0.0 6 36 2 213 66 0.0 6 64 2 214 65 0.0 6 65 215 72 0.0 6 68 4 216 45 0.0 6 45 217 52 0.0 6 50 2 218 77 0.0 6 76 1 219 49 0.0 6 49 220 34 0.0 6 34 221 43 0.0 6 43 222 139 0.0 6 135 4 223 130 0.0 6 127 3 224 76 0.0 6 74 2 225 68 0.0 6 67 1 226 93 0.0 6 91 2 227 55 0.0 6 53 2 228 47 0.0 6 46 1 229 48 0.0 6 48 230 44 0.0 6 43 1 231 65 0.0 6 65 232 44 0.0 6 41 3 233 62 0.0 6 62 234 39 0.0 6 38 0 1 235 38 0.0 6 36 2 236 88 0.0 6 86 2 237 157 0.0 6 154 3 238 157 0.0 6 148 9 239 146 0.0 6 145 1 240 88 0.0 6 85 2 1 241 213 0.0 6 211 2 242 89 0.0 6 87 2 243 98 0.0 6 95 2 1 244 75 0.0 6 72 3 245 131 0.0 6 129 2 246 70 0.0 6 69 1 247 100 0.0 6 99 1 248 108 0.0 6 104 3 1 249 89 0.0 6 88 1 250 128 0.0 6 128 251 96 0.0 6 93 3 252 64 0.0 6 63 1 253 50 0.0 6 49 1 254 72 0.0 6 72 255 138 0.0 6 136 2 256 172 0.0 6 169 3 257 95 0.0 6 93 1 1 258 135 0.0 6 130 5 259 254 0.0 6 249 5 260 177 0.0 6 176 1 261 101 0.0 6 100 1 262 50 0.0 6 49 1 263 83 0.0 6 81 2 264 80 0.0 6 77 2 1 265 138 0.0 6 136 2 266 91 0.0 6 88 2 1 267 66 0.0 6 65 1 268 347 0.0 6 345 2 269 210 0.0 6 204 6 270 213 0.0 6 210 3 271 85 0.0 6 84 1 272 160 0.0 6 154 5 1 273 437 0.0 6 423 14 274 242 0.0 6 239 3 275 250 0.0 6 246 4 276 352 0.0 6 342 9 1 277 309 0.0 6 295 13 1 278 5166 0.0 6 5049 117 279 449 0.0 6 423 26 280 11 0.0 6 9 2 281 1 0.0 6 1 283 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 15 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 1 0.0 6 1 75 1 0.0 6 1 78 1 0.0 6 1 88 2 0.0 6 2 91 1 0.0 6 1 100 1 0.0 6 1 102 1 0.0 6 1 110 1 0.0 6 1 111 1 0.0 6 1 126 1 0.0 6 1 127 1 0.0 6 1 203 1 0.0 6 1 231 1 0.0 6 1 257 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 15732 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 11 1 0.0 3 1 12 2 0.0 3 2 13 2 0.0 3 2 14 4 0.0 4 4 17 4 0.0 5 3 1 18 4 0.0 5 4 19 4 0.0 5 4 20 9 0.0 6 8 1 21 12 0.0 6 12 22 10 0.0 6 10 23 12 0.0 6 12 24 6 0.0 6 6 25 4 0.0 6 4 26 1 0.0 6 1 27 2 0.0 6 2 28 3 0.0 6 3 30 5 0.0 6 4 1 31 5 0.0 6 5 32 2 0.0 6 2 33 1 0.0 6 1 34 1 0.0 6 1 36 1 0.0 6 1 37 3 0.0 6 3 38 4 0.0 6 4 39 1 0.0 6 1 44 7 0.0 6 6 1 46 8 0.0 6 8 47 5 0.0 6 5 48 4 0.0 6 4 49 2 0.0 6 2 50 11 0.0 6 11 52 9 0.0 6 8 1 53 7 0.0 6 6 1 54 5 0.0 6 4 0 0 1 55 5 0.0 6 5 56 3 0.0 6 3 57 4 0.0 6 2 2 58 4 0.0 6 4 59 2 0.0 6 1 1 60 3 0.0 6 3 61 2 0.0 6 2 62 3 0.0 6 1 2 63 2 0.0 6 1 1 64 4 0.0 6 3 1 65 1 0.0 6 1 66 2 0.0 6 2 67 8 0.0 6 8 68 28 0.0 6 25 2 0 0 0 0 1 69 5 0.0 6 4 1 70 12 0.0 6 11 0 1 71 7 0.0 6 7 72 11 0.0 6 10 1 73 3 0.0 6 2 1 74 5 0.0 6 4 0 0 1 75 4 0.0 6 2 2 76 6 0.0 6 6 77 5 0.0 6 5 78 3 0.0 6 2 0 1 79 4 0.0 6 3 1 80 9 0.0 6 7 2 81 4 0.0 6 4 82 7 0.0 6 7 83 11 0.0 6 10 1 84 19 0.0 6 17 2 85 13 0.0 6 12 1 86 4 0.0 6 4 87 13 0.0 6 12 1 88 2 0.0 6 1 1 89 5 0.0 6 3 2 90 9 0.0 6 6 2 0 0 1 91 7 0.0 6 6 1 92 6 0.0 6 5 1 93 12 0.0 6 10 2 94 21 0.0 6 19 1 1 95 8 0.0 6 6 1 1 96 8 0.0 6 7 1 97 5 0.0 6 4 1 98 6 0.0 6 6 99 7 0.0 6 5 2 100 14 0.0 6 14 101 9 0.0 6 9 102 15 0.0 6 15 103 12 0.0 6 10 2 104 16 0.0 6 16 105 27 0.0 6 24 3 106 10 0.0 6 9 1 107 5 0.0 6 5 108 7 0.0 6 7 109 21 0.0 6 19 2 110 9 0.0 6 8 1 111 15 0.0 6 15 112 8 0.0 6 8 113 14 0.0 6 12 2 114 15 0.0 6 14 1 115 14 0.0 6 12 2 116 17 0.0 6 16 1 117 11 0.0 6 10 1 118 18 0.0 6 16 1 1 119 18 0.0 6 17 1 120 17 0.0 6 16 1 121 6 0.0 6 6 122 17 0.0 6 14 3 123 18 0.0 6 17 1 124 36 0.0 6 35 1 125 20 0.0 6 19 1 126 15 0.0 6 14 1 127 23 0.0 6 23 128 10 0.0 6 10 129 12 0.0 6 12 130 9 0.0 6 9 131 28 0.0 6 28 132 36 0.0 6 36 133 25 0.0 6 24 0 1 134 42 0.0 6 42 135 74 0.0 6 72 2 136 41 0.0 6 40 1 137 44 0.0 6 44 138 22 0.0 6 21 1 139 40 0.0 6 39 1 140 82 0.0 6 80 2 141 81 0.0 6 80 1 142 78 0.0 6 76 2 143 55 0.0 6 50 4 1 144 114 0.0 6 110 3 1 145 58 0.0 6 54 4 146 67 0.0 6 67 147 38 0.0 6 37 1 148 67 0.0 6 64 3 149 93 0.0 6 88 5 150 123 0.0 6 117 6 151 99 0.0 6 94 5 152 172 0.0 6 169 3 153 91 0.0 6 89 2 154 116 0.0 6 109 7 155 66 0.0 6 64 2 156 82 0.0 6 80 2 157 95 0.0 6 93 2 158 93 0.0 6 90 3 159 55 0.0 6 53 1 1 160 81 0.0 6 79 1 0 1 161 48 0.0 6 47 0 1 162 63 0.0 6 60 3 163 114 0.0 6 111 2 1 164 124 0.0 6 122 2 165 125 0.0 6 123 2 166 121 0.0 6 118 3 167 103 0.0 6 103 168 143 0.0 6 138 5 169 47 0.0 6 46 0 0 0 1 170 72 0.0 6 70 2 171 119 0.0 6 114 5 172 67 0.0 6 67 173 112 0.0 6 109 3 174 152 0.0 6 144 8 175 257 0.0 6 251 5 1 176 143 0.0 6 138 4 0 0 0 0 1 177 224 0.0 6 222 2 178 118 0.0 6 115 3 179 141 0.0 6 138 2 1 180 130 0.0 6 125 5 181 200 0.0 6 196 4 182 67 0.0 6 65 2 183 261 0.0 6 255 6 184 82 0.0 6 79 3 185 122 0.0 6 121 1 186 398 0.0 6 389 8 1 187 257 0.0 6 247 8 2 188 273 0.0 6 265 8 189 218 0.0 6 210 8 190 418 0.0 6 410 8 191 371 0.0 6 365 6 192 311 0.0 6 304 7 193 171 0.0 6 168 2 0 0 0 0 1 194 289 0.0 6 280 9 195 272 0.0 6 261 11 196 578 0.0 6 569 9 197 347 0.0 6 337 9 1 198 596 0.0 6 584 12 199 335 0.0 6 328 5 2 200 330 0.0 6 316 13 0 0 1 201 242 0.0 6 236 5 1 202 189 0.0 6 186 3 203 216 0.0 6 214 2 204 106 0.0 6 104 2 205 71 0.0 6 70 1 206 43 0.0 6 41 1 0 1 207 90 0.0 6 86 3 0 0 1 208 117 0.0 6 114 3 209 57 0.0 6 56 1 210 87 0.0 6 85 1 1 211 75 0.0 6 74 1 212 49 0.0 6 49 213 69 0.0 6 69 214 139 0.0 6 136 3 215 143 0.0 6 136 7 216 168 0.0 6 162 6 217 176 0.0 6 174 2 218 162 0.0 6 159 3 219 98 0.0 6 96 2 220 149 0.0 6 145 4 221 75 0.0 6 74 1 222 131 0.0 6 127 4 223 130 0.0 6 127 3 224 196 0.0 6 195 1 225 166 0.0 6 160 6 226 98 0.0 6 95 3 227 71 0.0 6 69 2 228 97 0.0 6 95 2 229 89 0.0 6 87 2 230 76 0.0 6 74 2 231 78 0.0 6 74 3 0 0 1 232 136 0.0 6 135 1 233 87 0.0 6 86 1 234 35 0.0 6 34 1 235 28 0.0 6 28 236 13 0.0 6 13 237 7 0.0 6 7 238 9 0.0 6 8 1 239 30 0.0 6 29 1 240 38 0.0 6 36 2 241 49 0.0 6 49 242 51 0.0 6 50 1 243 43 0.0 6 43 244 22 0.0 6 21 1 245 24 0.0 6 24 246 11 0.0 6 11 247 15 0.0 6 15 248 22 0.0 6 22 249 20 0.0 6 20 250 13 0.0 6 13 251 2 0.0 6 2 252 5 0.0 6 5 253 2 0.0 6 2 254 1 0.0 6 1 255 1 0.0 6 1 278 1 0.0 6 1 279 1 0.0 6 1 280 5 0.0 6 4 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 23 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 1 0.0 6 1 75 4 0.0 6 4 83 1 0.0 6 1 91 1 0.0 6 1 94 2 0.0 6 2 98 1 0.0 6 1 99 2 0.0 6 2 101 1 0.0 6 1 108 1 0.0 6 1 110 1 0.0 6 1 121 1 0.0 6 1 122 1 0.0 6 1 161 1 0.0 6 1 175 1 0.0 6 1 178 1 0.0 6 1 184 1 0.0 6 1 185 1 0.0 6 1 202 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_13_157_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_13_384_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_13_157_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_13_384_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.53 s (117 µs/read; 0.51 M reads/minute). === Summary === Total read pairs processed: 30,256 Read 1 with adapter: 30,250 (100.0%) Read 2 with adapter: 30,237 (99.9%) == Read fate breakdown == Pairs that were too short: 16 (0.1%) Pairs discarded as untrimmed: 25 (0.1%) Pairs written (passing filters): 30,215 (99.9%) Total basepairs processed: 12,861,197 bp Read 1: 7,194,560 bp Read 2: 5,666,637 bp Total written (filtered): 1,931,049 bp (15.0%) Read 1: 1,005,178 bp Read 2: 925,871 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 29442 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 472.8 0 0 1 4 2 118.2 1 1 1 5 2 29.5 1 2 7 2 1.8 2 1 0 1 8 1 0.5 2 1 9 1 0.1 2 0 1 13 1 0.0 3 1 16 2 0.0 4 1 1 17 1 0.0 5 0 1 19 2 0.0 5 2 20 23 0.0 6 22 1 21 18 0.0 6 17 1 22 15 0.0 6 14 1 23 8 0.0 6 8 24 5 0.0 6 5 25 7 0.0 6 7 26 8 0.0 6 7 1 27 8 0.0 6 8 28 2 0.0 6 2 29 2 0.0 6 2 31 2 0.0 6 2 32 4 0.0 6 4 33 6 0.0 6 6 34 2 0.0 6 1 1 36 3 0.0 6 2 1 37 1 0.0 6 1 39 4 0.0 6 4 40 4 0.0 6 4 41 4 0.0 6 4 42 3 0.0 6 3 43 1 0.0 6 1 44 6 0.0 6 4 2 45 2 0.0 6 2 46 1 0.0 6 1 48 3 0.0 6 3 49 2 0.0 6 2 50 2 0.0 6 2 51 6 0.0 6 6 52 3 0.0 6 3 53 3 0.0 6 3 54 1 0.0 6 1 55 1 0.0 6 1 56 3 0.0 6 2 0 1 57 1 0.0 6 1 58 4 0.0 6 4 59 3 0.0 6 3 60 2 0.0 6 2 61 5 0.0 6 4 1 62 2 0.0 6 2 63 5 0.0 6 4 1 64 4 0.0 6 4 65 2 0.0 6 2 66 2 0.0 6 2 67 5 0.0 6 5 68 1 0.0 6 1 69 3 0.0 6 3 70 4 0.0 6 3 0 0 0 0 0 1 71 3 0.0 6 3 72 4 0.0 6 3 1 73 2 0.0 6 2 74 4 0.0 6 4 75 4 0.0 6 3 1 76 2 0.0 6 2 77 2 0.0 6 1 1 78 5 0.0 6 5 79 10 0.0 6 9 1 80 7 0.0 6 7 81 4 0.0 6 4 82 3 0.0 6 3 83 2 0.0 6 2 84 8 0.0 6 8 85 6 0.0 6 6 86 4 0.0 6 4 87 4 0.0 6 4 88 2 0.0 6 1 0 0 0 1 89 1 0.0 6 1 90 2 0.0 6 1 1 92 5 0.0 6 4 1 93 3 0.0 6 2 1 94 5 0.0 6 5 95 5 0.0 6 5 96 5 0.0 6 5 97 3 0.0 6 3 98 4 0.0 6 4 99 8 0.0 6 8 100 4 0.0 6 4 101 8 0.0 6 7 1 102 11 0.0 6 10 1 103 10 0.0 6 10 104 15 0.0 6 15 105 19 0.0 6 18 0 0 0 1 106 10 0.0 6 10 107 10 0.0 6 10 108 8 0.0 6 8 109 11 0.0 6 11 110 11 0.0 6 11 111 3 0.0 6 2 1 112 8 0.0 6 7 1 113 16 0.0 6 16 114 9 0.0 6 9 115 11 0.0 6 11 116 7 0.0 6 7 117 8 0.0 6 7 1 118 10 0.0 6 10 119 8 0.0 6 7 1 120 5 0.0 6 5 121 8 0.0 6 8 122 14 0.0 6 13 1 123 10 0.0 6 10 124 10 0.0 6 9 1 125 13 0.0 6 13 126 8 0.0 6 7 1 127 16 0.0 6 16 128 20 0.0 6 19 0 1 129 19 0.0 6 18 1 130 19 0.0 6 19 131 15 0.0 6 14 1 132 15 0.0 6 15 133 19 0.0 6 19 134 24 0.0 6 23 1 135 18 0.0 6 18 136 14 0.0 6 14 137 24 0.0 6 23 1 138 21 0.0 6 21 139 38 0.0 6 38 140 79 0.0 6 79 141 372 0.0 6 372 142 185 0.0 6 183 2 143 565 0.0 6 564 1 144 262 0.0 6 262 145 96 0.0 6 95 1 146 409 0.0 6 408 1 147 300 0.0 6 299 1 148 173 0.0 6 172 1 149 81 0.0 6 80 1 150 129 0.0 6 128 1 151 151 0.0 6 151 152 77 0.0 6 76 1 153 99 0.0 6 99 154 86 0.0 6 85 1 155 28 0.0 6 28 156 37 0.0 6 37 157 50 0.0 6 49 1 158 60 0.0 6 59 0 0 0 0 0 1 159 56 0.0 6 56 160 126 0.0 6 125 1 161 310 0.0 6 309 0 1 162 398 0.0 6 397 1 163 103 0.0 6 103 164 128 0.0 6 126 1 0 1 165 71 0.0 6 69 2 166 73 0.0 6 71 2 167 202 0.0 6 201 1 168 137 0.0 6 136 0 0 0 0 1 169 90 0.0 6 90 170 321 0.0 6 319 0 0 1 0 1 171 78 0.0 6 78 172 77 0.0 6 77 173 57 0.0 6 56 1 174 166 0.0 6 166 175 101 0.0 6 99 1 0 0 0 1 176 112 0.0 6 112 177 168 0.0 6 168 178 769 0.0 6 769 179 315 0.0 6 313 2 180 133 0.0 6 133 181 108 0.0 6 106 1 1 182 101 0.0 6 98 2 1 183 162 0.0 6 161 1 184 146 0.0 6 142 3 0 0 0 0 1 185 534 0.0 6 530 3 0 0 1 186 166 0.0 6 163 3 187 187 0.0 6 184 3 188 106 0.0 6 106 189 184 0.0 6 182 0 0 0 0 2 190 128 0.0 6 126 2 191 274 0.0 6 273 1 192 135 0.0 6 134 1 193 97 0.0 6 94 3 194 170 0.0 6 168 1 0 0 0 1 195 231 0.0 6 230 1 196 219 0.0 6 218 1 197 309 0.0 6 305 4 198 314 0.0 6 312 2 199 194 0.0 6 192 2 200 174 0.0 6 171 2 0 0 0 1 201 229 0.0 6 228 1 202 201 0.0 6 199 2 203 238 0.0 6 238 204 233 0.0 6 231 2 205 441 0.0 6 437 3 0 1 206 387 0.0 6 383 3 0 0 1 207 369 0.0 6 368 1 208 394 0.0 6 390 4 209 228 0.0 6 226 2 210 301 0.0 6 297 4 211 360 0.0 6 357 3 212 387 0.0 6 381 5 0 0 0 0 1 213 470 0.0 6 465 5 214 318 0.0 6 312 6 215 453 0.0 6 449 4 216 268 0.0 6 263 3 2 217 335 0.0 6 329 5 0 0 1 218 340 0.0 6 337 3 219 370 0.0 6 366 2 0 0 0 1 1 220 295 0.0 6 294 1 221 288 0.0 6 284 2 1 0 1 222 261 0.0 6 259 2 223 224 0.0 6 223 1 224 271 0.0 6 269 2 225 277 0.0 6 276 1 226 302 0.0 6 299 3 227 343 0.0 6 340 3 228 205 0.0 6 203 2 229 155 0.0 6 154 1 230 205 0.0 6 205 231 245 0.0 6 245 232 244 0.0 6 243 1 233 274 0.0 6 273 1 234 205 0.0 6 201 3 0 0 0 0 1 235 167 0.0 6 166 1 236 226 0.0 6 224 2 237 314 0.0 6 314 238 264 0.0 6 263 1 239 366 0.0 6 365 1 240 208 0.0 6 207 1 241 153 0.0 6 151 1 0 0 1 242 190 0.0 6 190 243 193 0.0 6 190 2 0 0 0 0 1 244 168 0.0 6 166 1 1 245 153 0.0 6 152 1 246 224 0.0 6 223 0 0 1 247 124 0.0 6 124 248 146 0.0 6 146 249 141 0.0 6 139 1 0 1 250 137 0.0 6 137 251 115 0.0 6 115 252 100 0.0 6 99 1 253 98 0.0 6 98 254 76 0.0 6 76 255 70 0.0 6 70 256 103 0.0 6 101 2 257 106 0.0 6 103 2 0 0 0 1 258 96 0.0 6 96 259 129 0.0 6 129 260 146 0.0 6 145 1 261 68 0.0 6 68 262 79 0.0 6 79 263 45 0.0 6 45 264 58 0.0 6 57 1 265 83 0.0 6 83 266 75 0.0 6 73 2 267 84 0.0 6 84 268 62 0.0 6 61 1 269 74 0.0 6 73 0 0 0 0 1 270 66 0.0 6 66 271 67 0.0 6 67 272 79 0.0 6 78 0 0 0 0 1 273 51 0.0 6 50 1 274 53 0.0 6 53 275 112 0.0 6 112 276 114 0.0 6 113 0 0 1 277 200 0.0 6 195 2 0 1 0 2 278 509 0.0 6 505 3 0 0 0 1 279 1192 0.0 6 1187 5 280 5 0.0 6 5 282 2 0.0 6 2 289 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 808 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 3 0.0 6 3 72 13 0.0 6 13 73 6 0.0 6 6 74 16 0.0 6 16 75 3 0.0 6 3 76 3 0.0 6 3 77 16 0.0 6 16 78 9 0.0 6 9 79 6 0.0 6 6 80 5 0.0 6 5 81 5 0.0 6 5 82 3 0.0 6 3 84 3 0.0 6 3 88 2 0.0 6 2 89 1 0.0 6 1 90 1 0.0 6 1 91 8 0.0 6 8 92 12 0.0 6 12 93 9 0.0 6 9 94 1 0.0 6 1 95 3 0.0 6 3 96 2 0.0 6 2 98 6 0.0 6 6 100 3 0.0 6 3 101 11 0.0 6 11 102 1 0.0 6 1 104 1 0.0 6 1 107 2 0.0 6 2 108 10 0.0 6 10 109 26 0.0 6 26 110 9 0.0 6 9 111 4 0.0 6 4 112 2 0.0 6 2 113 3 0.0 6 3 114 3 0.0 6 3 115 5 0.0 6 5 116 12 0.0 6 12 117 2 0.0 6 2 118 5 0.0 6 5 119 3 0.0 6 3 120 4 0.0 6 4 121 1 0.0 6 1 122 6 0.0 6 6 123 1 0.0 6 1 124 2 0.0 6 2 125 4 0.0 6 4 126 4 0.0 6 4 127 1 0.0 6 1 128 3 0.0 6 3 129 3 0.0 6 3 131 5 0.0 6 5 132 3 0.0 6 3 133 3 0.0 6 3 134 1 0.0 6 1 135 3 0.0 6 3 136 8 0.0 6 8 137 1 0.0 6 1 138 6 0.0 6 6 139 9 0.0 6 9 140 2 0.0 6 2 141 7 0.0 6 7 142 8 0.0 6 8 143 7 0.0 6 7 144 2 0.0 6 2 145 1 0.0 6 1 147 1 0.0 6 1 148 5 0.0 6 5 149 8 0.0 6 8 150 5 0.0 6 5 151 1 0.0 6 1 152 7 0.0 6 7 153 7 0.0 6 7 154 1 0.0 6 1 155 5 0.0 6 5 156 1 0.0 6 1 157 7 0.0 6 7 158 14 0.0 6 14 159 4 0.0 6 4 162 4 0.0 6 4 163 7 0.0 6 7 164 11 0.0 6 11 166 1 0.0 6 1 167 4 0.0 6 4 168 3 0.0 6 3 169 14 0.0 6 14 170 5 0.0 6 5 171 2 0.0 6 2 173 3 0.0 6 3 174 7 0.0 6 7 175 3 0.0 6 3 176 12 0.0 6 12 177 19 0.0 6 19 178 5 0.0 6 5 179 5 0.0 6 5 181 1 0.0 6 1 182 1 0.0 6 1 183 9 0.0 6 9 184 21 0.0 6 20 1 185 7 0.0 6 7 186 2 0.0 6 2 187 4 0.0 6 4 188 11 0.0 6 11 189 3 0.0 6 3 190 4 0.0 6 4 191 2 0.0 6 2 192 1 0.0 6 1 193 4 0.0 6 4 194 2 0.0 6 2 195 1 0.0 6 1 196 1 0.0 6 1 197 6 0.0 6 6 198 2 0.0 6 2 200 5 0.0 6 5 201 10 0.0 6 10 202 10 0.0 6 10 203 14 0.0 6 14 204 4 0.0 6 4 205 3 0.0 6 3 206 1 0.0 6 1 207 3 0.0 6 3 208 1 0.0 6 1 209 3 0.0 6 3 210 4 0.0 6 4 211 6 0.0 6 6 212 13 0.0 6 13 213 26 0.0 6 26 214 11 0.0 6 11 215 3 0.0 6 3 220 3 0.0 6 3 221 7 0.0 6 7 222 1 0.0 6 1 223 5 0.0 6 5 224 2 0.0 6 1 1 226 4 0.0 6 4 228 3 0.0 6 3 229 1 0.0 6 1 230 9 0.0 6 8 0 0 0 0 0 1 231 4 0.0 6 4 232 5 0.0 6 5 233 4 0.0 6 4 234 3 0.0 6 3 237 1 0.0 6 1 239 2 0.0 6 2 240 5 0.0 6 5 242 3 0.0 6 3 243 3 0.0 6 3 246 2 0.0 6 2 247 1 0.0 6 1 248 1 0.0 6 1 249 2 0.0 6 2 250 1 0.0 6 1 253 5 0.0 6 5 254 3 0.0 6 3 256 3 0.0 6 3 257 5 0.0 6 5 259 1 0.0 6 1 260 4 0.0 6 4 261 4 0.0 6 4 267 1 0.0 6 1 272 1 0.0 6 1 275 1 0.0 6 1 276 1 0.0 6 1 278 1 0.0 6 1 279 1 0.0 6 1 292 1 0.0 6 1 295 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 29581 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 9 1 0.1 2 1 11 1 0.0 3 1 12 2 0.0 3 2 13 10 0.0 3 9 1 14 4 0.0 4 3 0 0 1 16 2 0.0 4 2 17 5 0.0 5 5 18 3 0.0 5 1 2 19 9 0.0 5 8 1 20 22 0.0 6 20 2 21 15 0.0 6 14 1 22 8 0.0 6 8 23 10 0.0 6 10 24 5 0.0 6 5 25 9 0.0 6 9 26 10 0.0 6 9 1 27 12 0.0 6 12 28 7 0.0 6 7 29 5 0.0 6 5 30 10 0.0 6 10 31 2 0.0 6 1 1 32 7 0.0 6 6 1 33 7 0.0 6 7 34 5 0.0 6 4 1 35 3 0.0 6 3 36 7 0.0 6 7 37 6 0.0 6 6 38 5 0.0 6 4 1 39 9 0.0 6 8 1 40 4 0.0 6 4 41 2 0.0 6 2 42 8 0.0 6 7 1 43 6 0.0 6 6 44 6 0.0 6 5 1 45 4 0.0 6 4 46 5 0.0 6 5 47 5 0.0 6 4 1 48 5 0.0 6 5 49 8 0.0 6 8 50 7 0.0 6 6 1 51 9 0.0 6 8 1 52 5 0.0 6 4 1 53 11 0.0 6 11 54 6 0.0 6 6 55 7 0.0 6 7 56 10 0.0 6 8 1 0 0 0 0 1 57 10 0.0 6 8 1 1 58 4 0.0 6 4 59 12 0.0 6 11 1 60 9 0.0 6 9 61 11 0.0 6 10 1 62 7 0.0 6 7 63 9 0.0 6 9 64 12 0.0 6 11 1 65 14 0.0 6 12 2 66 12 0.0 6 9 2 0 1 67 11 0.0 6 11 68 25 0.0 6 25 69 54 0.0 6 52 2 70 25 0.0 6 23 2 71 38 0.0 6 36 2 72 16 0.0 6 15 1 73 23 0.0 6 19 3 0 0 1 74 13 0.0 6 13 75 14 0.0 6 13 1 76 16 0.0 6 15 1 77 6 0.0 6 6 78 12 0.0 6 12 79 22 0.0 6 22 80 27 0.0 6 27 81 18 0.0 6 15 0 1 0 0 0 2 82 36 0.0 6 34 2 83 39 0.0 6 39 84 21 0.0 6 21 85 33 0.0 6 33 86 21 0.0 6 21 87 20 0.0 6 18 1 0 0 0 0 1 88 21 0.0 6 21 89 21 0.0 6 20 1 90 16 0.0 6 16 91 31 0.0 6 29 2 92 38 0.0 6 38 93 34 0.0 6 33 1 94 39 0.0 6 36 3 95 44 0.0 6 43 1 96 36 0.0 6 34 2 97 34 0.0 6 33 0 0 0 0 0 1 98 43 0.0 6 43 99 37 0.0 6 36 1 100 30 0.0 6 30 101 31 0.0 6 29 2 102 36 0.0 6 35 1 103 52 0.0 6 52 104 48 0.0 6 48 105 36 0.0 6 35 1 106 88 0.0 6 87 1 107 59 0.0 6 58 1 108 44 0.0 6 44 109 58 0.0 6 57 1 110 62 0.0 6 62 111 54 0.0 6 54 112 68 0.0 6 65 2 1 113 63 0.0 6 61 2 114 74 0.0 6 72 2 115 76 0.0 6 73 3 116 78 0.0 6 74 4 117 84 0.0 6 80 2 0 1 1 118 91 0.0 6 85 5 0 0 0 1 119 77 0.0 6 75 1 1 120 97 0.0 6 95 2 121 87 0.0 6 85 2 122 93 0.0 6 89 3 0 0 1 123 106 0.0 6 101 4 1 124 95 0.0 6 92 2 1 125 123 0.0 6 122 1 126 103 0.0 6 103 127 131 0.0 6 130 1 128 121 0.0 6 115 6 129 124 0.0 6 118 6 130 161 0.0 6 160 1 131 142 0.0 6 138 3 0 0 1 132 126 0.0 6 123 3 133 159 0.0 6 154 5 134 205 0.0 6 200 4 0 1 135 179 0.0 6 172 7 136 208 0.0 6 203 5 137 239 0.0 6 235 3 0 0 1 138 204 0.0 6 200 2 1 0 0 0 1 139 288 0.0 6 278 10 140 536 0.0 6 532 4 141 380 0.0 6 371 9 142 624 0.0 6 617 4 0 2 1 143 479 0.0 6 476 3 144 419 0.0 6 408 9 1 1 145 583 0.0 6 577 5 1 146 508 0.0 6 502 6 147 407 0.0 6 400 4 0 1 2 148 399 0.0 6 392 7 149 376 0.0 6 371 3 0 0 1 0 1 150 503 0.0 6 499 4 151 487 0.0 6 474 12 1 152 640 0.0 6 625 13 0 0 2 153 445 0.0 6 435 8 0 1 0 1 154 569 0.0 6 557 10 0 1 0 0 1 155 486 0.0 6 481 4 0 0 1 156 458 0.0 6 449 5 1 0 2 0 1 157 498 0.0 6 488 8 1 0 0 0 1 158 487 0.0 6 482 5 159 555 0.0 6 547 6 1 1 160 644 0.0 6 634 8 1 0 1 161 560 0.0 6 551 8 0 1 162 419 0.0 6 413 6 163 353 0.0 6 346 7 164 361 0.0 6 352 8 0 0 1 165 372 0.0 6 366 4 0 0 2 166 407 0.0 6 400 5 1 0 0 1 167 401 0.0 6 397 3 1 168 418 0.0 6 411 3 1 1 2 169 396 0.0 6 392 1 2 0 1 170 417 0.0 6 412 4 0 0 1 171 343 0.0 6 337 4 1 0 1 172 320 0.0 6 316 4 173 441 0.0 6 438 2 0 0 0 1 174 386 0.0 6 381 5 175 433 0.0 6 427 5 0 0 0 0 1 176 498 0.0 6 494 4 177 531 0.0 6 522 7 0 0 1 0 1 178 389 0.0 6 388 1 179 282 0.0 6 279 2 0 0 1 180 311 0.0 6 305 6 181 243 0.0 6 241 1 0 0 0 0 1 182 178 0.0 6 177 1 183 247 0.0 6 246 1 184 348 0.0 6 345 3 185 274 0.0 6 269 4 1 186 409 0.0 6 407 2 187 326 0.0 6 320 5 1 188 226 0.0 6 225 1 189 198 0.0 6 197 0 0 0 0 1 190 221 0.0 6 218 3 191 175 0.0 6 173 2 192 218 0.0 6 217 1 193 165 0.0 6 164 1 194 164 0.0 6 162 1 1 195 123 0.0 6 123 196 124 0.0 6 121 2 1 197 88 0.0 6 86 1 0 0 0 1 198 105 0.0 6 105 199 118 0.0 6 116 1 0 0 1 200 117 0.0 6 117 201 98 0.0 6 98 202 88 0.0 6 87 1 203 87 0.0 6 86 0 0 0 0 1 204 86 0.0 6 85 0 0 0 1 205 72 0.0 6 72 206 79 0.0 6 77 1 0 0 0 0 1 207 74 0.0 6 74 208 61 0.0 6 61 209 71 0.0 6 71 210 43 0.0 6 43 211 53 0.0 6 53 212 44 0.0 6 44 213 50 0.0 6 50 214 52 0.0 6 52 215 54 0.0 6 51 3 216 63 0.0 6 61 1 0 0 0 1 217 64 0.0 6 64 218 37 0.0 6 37 219 29 0.0 6 28 1 220 34 0.0 6 34 221 9 0.0 6 9 222 20 0.0 6 20 223 17 0.0 6 17 224 23 0.0 6 23 225 18 0.0 6 18 226 15 0.0 6 15 227 15 0.0 6 15 228 11 0.0 6 11 229 6 0.0 6 6 230 6 0.0 6 6 231 8 0.0 6 8 232 4 0.0 6 4 233 12 0.0 6 12 234 3 0.0 6 3 235 1 0.0 6 1 237 1 0.0 6 1 239 2 0.0 6 2 240 1 0.0 6 1 241 2 0.0 6 2 242 2 0.0 6 2 243 2 0.0 6 2 244 1 0.0 6 1 247 2 0.0 6 2 249 1 0.0 6 1 251 1 0.0 6 1 252 1 0.0 6 1 255 1 0.0 6 1 256 1 0.0 6 1 257 1 0.0 6 1 279 4 0.0 6 4 280 5 0.0 6 5 281 64 0.0 6 62 1 0 1 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 656 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 68 4 0.0 6 4 69 1 0.0 6 1 70 13 0.0 6 13 71 6 0.0 6 6 72 24 0.0 6 24 73 6 0.0 6 6 74 4 0.0 6 4 75 19 0.0 6 19 76 9 0.0 6 9 77 4 0.0 6 4 78 1 0.0 6 1 79 3 0.0 6 3 80 5 0.0 6 5 81 3 0.0 6 3 82 4 0.0 6 4 83 5 0.0 6 5 85 1 0.0 6 1 89 2 0.0 6 2 90 15 0.0 6 15 91 11 0.0 6 11 92 4 0.0 6 4 93 4 0.0 6 4 94 3 0.0 6 3 95 3 0.0 6 3 96 10 0.0 6 10 97 2 0.0 6 2 98 2 0.0 6 2 99 5 0.0 6 5 101 1 0.0 6 1 102 1 0.0 6 1 103 1 0.0 6 1 104 1 0.0 6 1 105 3 0.0 6 3 106 11 0.0 6 11 107 29 0.0 6 29 108 14 0.0 6 14 109 3 0.0 6 3 110 1 0.0 6 1 111 1 0.0 6 1 112 2 0.0 6 2 113 1 0.0 6 1 114 23 0.0 6 23 115 2 0.0 6 2 116 2 0.0 6 2 117 1 0.0 6 1 118 1 0.0 6 1 119 2 0.0 6 2 120 12 0.0 6 12 121 1 0.0 6 1 122 2 0.0 6 2 123 2 0.0 6 2 124 1 0.0 6 1 125 2 0.0 6 2 126 10 0.0 6 10 127 3 0.0 6 3 129 1 0.0 6 1 130 4 0.0 6 4 131 5 0.0 6 5 133 1 0.0 6 1 134 13 0.0 6 13 135 4 0.0 6 4 136 7 0.0 6 7 137 11 0.0 6 11 138 2 0.0 6 2 139 6 0.0 6 6 140 9 0.0 6 9 141 7 0.0 6 7 142 3 0.0 6 2 0 0 0 1 144 2 0.0 6 2 145 1 0.0 6 1 146 6 0.0 6 6 147 4 0.0 6 4 148 6 0.0 6 6 149 2 0.0 6 2 150 11 0.0 6 11 151 11 0.0 6 11 153 2 0.0 6 2 154 4 0.0 6 4 155 2 0.0 6 1 0 0 0 1 156 12 0.0 6 12 157 4 0.0 6 4 159 2 0.0 6 2 160 3 0.0 6 3 161 10 0.0 6 10 162 5 0.0 6 5 164 1 0.0 6 1 165 3 0.0 6 3 166 4 0.0 6 4 167 14 0.0 6 14 168 6 0.0 6 6 169 2 0.0 6 2 170 4 0.0 6 4 171 1 0.0 6 1 172 12 0.0 6 11 0 0 0 1 173 2 0.0 6 2 174 6 0.0 6 6 175 18 0.0 6 18 177 7 0.0 6 7 179 2 0.0 6 2 180 5 0.0 6 5 181 5 0.0 6 5 182 8 0.0 6 8 183 5 0.0 6 5 185 3 0.0 6 3 186 8 0.0 6 8 188 2 0.0 6 1 0 0 1 189 5 0.0 6 5 190 1 0.0 6 1 192 3 0.0 6 3 194 1 0.0 6 1 195 2 0.0 6 2 196 1 0.0 6 1 197 1 0.0 6 1 198 2 0.0 6 2 199 9 0.0 6 9 200 5 0.0 6 5 201 13 0.0 6 13 202 1 0.0 6 1 205 1 0.0 6 1 206 1 0.0 6 1 208 1 0.0 6 1 210 3 0.0 6 3 211 10 0.0 6 10 212 3 0.0 6 3 217 1 0.0 6 1 221 2 0.0 6 2 222 3 0.0 6 3 224 1 0.0 6 1 228 1 0.0 6 1 251 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_08_158_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_08_385_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_08_158_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_08_385_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.16 s (58 µs/read; 1.04 M reads/minute). === Summary === Total read pairs processed: 89,633 Read 1 with adapter: 89,617 (100.0%) Read 2 with adapter: 89,633 (100.0%) == Read fate breakdown == Pairs that were too short: 41 (0.0%) Pairs discarded as untrimmed: 16 (0.0%) Pairs written (passing filters): 89,576 (99.9%) Total basepairs processed: 37,682,426 bp Read 1: 21,050,295 bp Read 2: 16,632,131 bp Total written (filtered): 5,721,405 bp (15.2%) Read 1: 2,894,928 bp Read 2: 2,826,477 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 87312 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1400.5 0 2 4 3 350.1 1 1 2 5 1 87.5 1 1 6 1 21.9 1 0 0 1 7 4 5.5 2 2 0 2 8 1 1.4 2 1 9 1 0.3 2 1 10 1 0.1 3 1 14 2 0.0 4 2 15 3 0.0 4 0 1 2 16 6 0.0 4 4 1 1 19 3 0.0 5 1 2 20 146 0.0 6 143 3 21 51 0.0 6 50 0 1 22 51 0.0 6 50 1 23 46 0.0 6 46 24 30 0.0 6 30 25 25 0.0 6 25 26 17 0.0 6 17 27 11 0.0 6 10 1 28 10 0.0 6 9 1 29 13 0.0 6 12 1 30 8 0.0 6 7 1 31 6 0.0 6 6 32 4 0.0 6 4 33 6 0.0 6 6 34 10 0.0 6 10 35 9 0.0 6 8 0 0 0 0 0 1 36 14 0.0 6 13 1 37 5 0.0 6 5 38 7 0.0 6 7 39 12 0.0 6 11 1 40 16 0.0 6 15 1 41 10 0.0 6 9 1 42 8 0.0 6 7 0 0 0 0 1 43 6 0.0 6 5 1 44 12 0.0 6 12 45 5 0.0 6 4 1 46 4 0.0 6 4 47 3 0.0 6 3 48 3 0.0 6 3 49 6 0.0 6 6 50 5 0.0 6 4 1 51 17 0.0 6 17 52 7 0.0 6 7 53 10 0.0 6 9 1 54 2 0.0 6 1 1 55 6 0.0 6 5 0 1 56 5 0.0 6 5 57 9 0.0 6 8 1 58 4 0.0 6 4 59 1 0.0 6 1 60 5 0.0 6 4 1 61 8 0.0 6 8 62 4 0.0 6 2 2 63 8 0.0 6 8 64 10 0.0 6 9 0 0 0 0 0 1 65 8 0.0 6 8 66 11 0.0 6 10 1 67 10 0.0 6 10 68 10 0.0 6 10 69 8 0.0 6 8 70 12 0.0 6 12 71 12 0.0 6 12 72 7 0.0 6 7 73 14 0.0 6 14 74 14 0.0 6 14 75 9 0.0 6 9 76 18 0.0 6 17 1 77 10 0.0 6 9 1 78 10 0.0 6 10 79 12 0.0 6 11 1 80 7 0.0 6 7 81 11 0.0 6 11 82 11 0.0 6 11 83 13 0.0 6 13 84 23 0.0 6 22 1 85 17 0.0 6 17 86 21 0.0 6 20 1 87 8 0.0 6 6 2 88 15 0.0 6 14 1 89 18 0.0 6 17 1 90 13 0.0 6 13 91 10 0.0 6 10 92 9 0.0 6 8 1 93 18 0.0 6 17 1 94 14 0.0 6 13 1 95 11 0.0 6 11 96 10 0.0 6 8 1 0 0 0 1 97 6 0.0 6 6 98 16 0.0 6 16 99 15 0.0 6 15 100 7 0.0 6 7 101 16 0.0 6 16 102 18 0.0 6 15 2 1 103 26 0.0 6 25 1 104 22 0.0 6 22 105 30 0.0 6 28 2 106 26 0.0 6 22 3 0 1 107 20 0.0 6 19 1 108 21 0.0 6 20 1 109 24 0.0 6 23 1 110 20 0.0 6 19 0 0 0 0 0 1 111 28 0.0 6 27 0 0 0 0 1 112 14 0.0 6 14 113 23 0.0 6 20 3 114 27 0.0 6 27 115 27 0.0 6 27 116 18 0.0 6 17 1 117 37 0.0 6 37 118 15 0.0 6 15 119 27 0.0 6 26 1 120 28 0.0 6 28 121 27 0.0 6 27 122 28 0.0 6 28 123 31 0.0 6 29 2 124 33 0.0 6 32 0 0 0 0 1 125 39 0.0 6 37 1 0 0 0 1 126 42 0.0 6 42 127 24 0.0 6 23 1 128 38 0.0 6 35 3 129 55 0.0 6 54 1 130 48 0.0 6 48 131 46 0.0 6 46 132 48 0.0 6 48 133 51 0.0 6 49 2 134 47 0.0 6 46 1 135 65 0.0 6 65 136 54 0.0 6 53 1 137 65 0.0 6 65 138 58 0.0 6 58 139 51 0.0 6 50 1 140 68 0.0 6 68 141 100 0.0 6 98 2 142 245 0.0 6 243 2 143 462 0.0 6 460 1 0 0 1 144 592 0.0 6 591 1 145 365 0.0 6 365 146 376 0.0 6 375 1 147 584 0.0 6 583 1 148 366 0.0 6 365 0 1 149 513 0.0 6 511 1 0 1 150 518 0.0 6 518 151 437 0.0 6 436 1 152 441 0.0 6 440 1 153 368 0.0 6 368 154 354 0.0 6 350 2 0 1 1 155 372 0.0 6 371 1 156 461 0.0 6 459 2 157 345 0.0 6 345 158 325 0.0 6 324 1 159 418 0.0 6 418 160 444 0.0 6 441 3 161 404 0.0 6 402 2 162 494 0.0 6 493 1 163 486 0.0 6 485 1 164 395 0.0 6 395 165 468 0.0 6 468 166 347 0.0 6 346 1 167 384 0.0 6 381 2 1 168 529 0.0 6 529 169 385 0.0 6 383 2 170 445 0.0 6 445 171 507 0.0 6 506 0 0 1 172 422 0.0 6 417 2 0 3 173 562 0.0 6 559 2 0 1 174 567 0.0 6 566 1 175 448 0.0 6 448 176 507 0.0 6 507 177 542 0.0 6 541 1 178 431 0.0 6 431 179 595 0.0 6 595 180 1606 0.0 6 1603 3 181 620 0.0 6 619 1 182 511 0.0 6 509 1 1 183 557 0.0 6 554 3 184 512 0.0 6 511 1 185 484 0.0 6 482 2 186 609 0.0 6 607 2 187 661 0.0 6 657 3 0 0 1 188 621 0.0 6 620 1 189 606 0.0 6 605 0 0 0 0 1 190 555 0.0 6 552 3 191 628 0.0 6 622 6 192 679 0.0 6 678 0 0 0 0 0 1 193 661 0.0 6 657 3 0 0 1 194 863 0.0 6 860 3 195 1051 0.0 6 1048 3 196 1070 0.0 6 1064 5 1 197 1066 0.0 6 1064 2 198 1399 0.0 6 1392 6 0 0 0 0 1 199 1345 0.0 6 1343 2 200 1073 0.0 6 1067 4 0 0 1 0 1 201 998 0.0 6 996 2 202 959 0.0 6 955 4 203 962 0.0 6 956 5 0 0 0 1 204 1359 0.0 6 1355 2 0 0 1 1 205 907 0.0 6 906 1 206 1068 0.0 6 1063 3 0 0 1 0 1 207 1397 0.0 6 1392 5 208 1075 0.0 6 1072 2 0 0 0 1 209 1171 0.0 6 1166 4 1 210 932 0.0 6 929 2 0 0 0 1 211 940 0.0 6 935 5 212 1068 0.0 6 1062 6 213 1170 0.0 6 1165 5 214 1072 0.0 6 1066 5 1 215 1286 0.0 6 1281 4 0 0 0 1 216 1520 0.0 6 1512 8 217 1099 0.0 6 1096 2 1 218 1152 0.0 6 1145 6 0 0 0 0 1 219 1190 0.0 6 1184 6 220 1073 0.0 6 1067 4 0 0 0 1 1 221 980 0.0 6 976 4 222 944 0.0 6 939 5 223 839 0.0 6 837 1 1 224 923 0.0 6 920 3 225 980 0.0 6 978 2 226 1065 0.0 6 1065 227 1041 0.0 6 1038 3 228 1009 0.0 6 1006 2 1 229 770 0.0 6 765 4 1 230 736 0.0 6 732 4 231 902 0.0 6 899 3 232 772 0.0 6 767 3 1 0 1 233 833 0.0 6 826 6 0 0 1 234 812 0.0 6 810 2 235 656 0.0 6 652 3 0 0 0 1 236 561 0.0 6 559 2 237 644 0.0 6 643 1 238 534 0.0 6 531 3 239 566 0.0 6 565 1 240 588 0.0 6 584 3 0 0 0 1 241 548 0.0 6 546 1 0 0 1 242 574 0.0 6 573 0 0 0 0 1 243 613 0.0 6 611 2 244 467 0.0 6 465 2 245 448 0.0 6 447 1 246 366 0.0 6 363 3 247 384 0.0 6 384 248 344 0.0 6 344 249 343 0.0 6 342 1 250 341 0.0 6 339 1 0 1 251 298 0.0 6 298 252 312 0.0 6 311 0 0 1 253 270 0.0 6 269 1 254 252 0.0 6 251 1 255 254 0.0 6 254 256 275 0.0 6 275 257 263 0.0 6 263 258 209 0.0 6 209 259 224 0.0 6 222 2 260 232 0.0 6 231 1 261 203 0.0 6 203 262 133 0.0 6 133 263 171 0.0 6 170 1 264 155 0.0 6 154 1 265 136 0.0 6 134 2 266 124 0.0 6 123 1 267 117 0.0 6 116 1 268 136 0.0 6 135 1 269 137 0.0 6 137 270 142 0.0 6 142 271 124 0.0 6 124 272 115 0.0 6 115 273 100 0.0 6 100 274 117 0.0 6 115 2 275 132 0.0 6 131 1 276 180 0.0 6 180 277 205 0.0 6 203 2 278 261 0.0 6 259 2 279 986 0.0 6 979 6 0 1 280 1373 0.0 6 1365 7 0 0 0 1 281 6 0.0 6 6 282 1 0.0 6 1 286 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2305 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 2 0.0 6 1 1 62 93 0.0 6 86 7 70 2 0.0 6 2 71 7 0.0 6 7 72 14 0.0 6 14 73 15 0.0 6 15 74 14 0.0 6 14 75 10 0.0 6 10 76 14 0.0 6 14 77 12 0.0 6 12 78 22 0.0 6 22 79 14 0.0 6 14 80 16 0.0 6 15 1 81 15 0.0 6 14 1 82 4 0.0 6 4 83 11 0.0 6 11 84 17 0.0 6 17 85 12 0.0 6 12 86 14 0.0 6 14 87 10 0.0 6 10 88 11 0.0 6 11 89 7 0.0 6 7 90 4 0.0 6 4 91 15 0.0 6 15 92 13 0.0 6 13 93 17 0.0 6 17 94 8 0.0 6 8 95 7 0.0 6 7 96 10 0.0 6 10 97 13 0.0 6 13 98 5 0.0 6 5 99 10 0.0 6 10 100 10 0.0 6 10 101 7 0.0 6 7 102 13 0.0 6 12 1 103 7 0.0 6 7 104 9 0.0 6 9 105 8 0.0 6 8 106 9 0.0 6 9 107 9 0.0 6 9 108 32 0.0 6 32 109 47 0.0 6 47 110 15 0.0 6 15 111 13 0.0 6 13 112 13 0.0 6 13 113 5 0.0 6 5 114 11 0.0 6 11 115 7 0.0 6 7 116 11 0.0 6 10 0 0 0 0 0 1 117 12 0.0 6 12 118 7 0.0 6 7 119 15 0.0 6 15 120 8 0.0 6 8 121 16 0.0 6 16 122 7 0.0 6 7 123 13 0.0 6 13 124 19 0.0 6 19 125 10 0.0 6 10 126 20 0.0 6 20 127 22 0.0 6 22 128 8 0.0 6 8 129 16 0.0 6 16 130 9 0.0 6 9 131 8 0.0 6 8 132 11 0.0 6 11 133 40 0.0 6 39 0 0 0 0 0 1 134 13 0.0 6 13 135 16 0.0 6 16 136 12 0.0 6 12 137 16 0.0 6 16 138 21 0.0 6 21 139 17 0.0 6 17 140 21 0.0 6 21 141 12 0.0 6 12 142 18 0.0 6 18 143 17 0.0 6 17 144 22 0.0 6 22 145 15 0.0 6 14 0 0 0 0 0 1 146 18 0.0 6 18 147 14 0.0 6 14 148 19 0.0 6 19 149 12 0.0 6 12 150 21 0.0 6 21 151 24 0.0 6 24 152 15 0.0 6 15 153 16 0.0 6 16 154 11 0.0 6 11 155 14 0.0 6 14 156 16 0.0 6 16 157 22 0.0 6 22 158 10 0.0 6 10 159 16 0.0 6 16 160 18 0.0 6 18 161 22 0.0 6 22 162 28 0.0 6 28 163 26 0.0 6 26 164 14 0.0 6 14 165 18 0.0 6 18 166 15 0.0 6 15 167 19 0.0 6 19 168 21 0.0 6 21 169 26 0.0 6 26 170 10 0.0 6 10 171 25 0.0 6 25 172 15 0.0 6 15 173 13 0.0 6 13 174 9 0.0 6 9 175 11 0.0 6 11 176 9 0.0 6 9 177 14 0.0 6 14 178 14 0.0 6 14 179 16 0.0 6 16 180 10 0.0 6 10 181 16 0.0 6 16 182 12 0.0 6 12 183 14 0.0 6 14 184 17 0.0 6 17 185 11 0.0 6 11 186 11 0.0 6 11 187 16 0.0 6 16 188 13 0.0 6 13 189 11 0.0 6 11 190 13 0.0 6 13 191 3 0.0 6 3 192 19 0.0 6 19 193 19 0.0 6 19 194 4 0.0 6 4 195 10 0.0 6 9 0 0 0 0 0 1 196 13 0.0 6 13 197 7 0.0 6 7 198 7 0.0 6 7 199 11 0.0 6 11 200 9 0.0 6 9 201 15 0.0 6 15 202 19 0.0 6 19 203 7 0.0 6 7 204 7 0.0 6 7 205 14 0.0 6 14 206 7 0.0 6 7 207 10 0.0 6 10 208 10 0.0 6 10 210 7 0.0 6 7 211 12 0.0 6 12 212 12 0.0 6 12 213 5 0.0 6 5 214 13 0.0 6 13 215 6 0.0 6 6 216 6 0.0 6 6 217 8 0.0 6 8 218 11 0.0 6 11 219 25 0.0 6 25 220 13 0.0 6 13 221 8 0.0 6 8 222 5 0.0 6 5 223 12 0.0 6 12 224 6 0.0 6 6 225 9 0.0 6 9 226 9 0.0 6 9 227 6 0.0 6 6 228 4 0.0 6 4 229 4 0.0 6 4 230 2 0.0 6 2 231 5 0.0 6 5 232 2 0.0 6 2 233 7 0.0 6 7 234 5 0.0 6 5 235 7 0.0 6 7 236 4 0.0 6 4 237 7 0.0 6 7 238 4 0.0 6 4 239 4 0.0 6 4 240 2 0.0 6 2 241 1 0.0 6 1 242 1 0.0 6 1 243 2 0.0 6 2 244 2 0.0 6 2 245 5 0.0 6 5 246 1 0.0 6 1 247 6 0.0 6 6 248 4 0.0 6 4 249 3 0.0 6 3 250 3 0.0 6 3 251 3 0.0 6 3 252 2 0.0 6 2 253 3 0.0 6 3 254 1 0.0 6 1 255 1 0.0 6 1 256 3 0.0 6 3 257 1 0.0 6 1 258 1 0.0 6 1 260 1 0.0 6 1 261 2 0.0 6 2 262 1 0.0 6 1 263 2 0.0 6 2 264 1 0.0 6 1 265 1 0.0 6 1 267 1 0.0 6 1 271 1 0.0 6 1 272 1 0.0 6 1 273 1 0.0 6 1 276 1 0.0 6 1 277 1 0.0 6 1 281 1 0.0 6 1 282 2 0.0 6 2 286 1 0.0 6 1 289 1 0.0 6 1 292 1 0.0 6 1 296 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 87627 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 21.9 1 1 10 2 0.1 3 2 11 5 0.0 3 4 1 12 6 0.0 3 6 13 10 0.0 3 10 14 10 0.0 4 10 15 3 0.0 4 2 0 1 16 7 0.0 4 7 17 31 0.0 5 31 18 24 0.0 5 18 4 2 19 16 0.0 5 15 0 1 20 80 0.0 6 75 5 21 55 0.0 6 51 4 22 29 0.0 6 27 2 23 29 0.0 6 29 24 32 0.0 6 30 2 25 15 0.0 6 12 3 26 19 0.0 6 18 1 27 17 0.0 6 17 28 9 0.0 6 9 29 17 0.0 6 15 1 0 0 0 0 1 30 12 0.0 6 12 31 9 0.0 6 8 1 32 20 0.0 6 19 1 33 11 0.0 6 10 1 34 14 0.0 6 13 1 35 16 0.0 6 15 1 36 17 0.0 6 16 1 37 17 0.0 6 14 3 38 14 0.0 6 13 0 1 39 12 0.0 6 10 2 40 10 0.0 6 9 1 41 22 0.0 6 21 1 42 19 0.0 6 15 3 0 1 43 21 0.0 6 20 0 1 44 14 0.0 6 12 2 45 15 0.0 6 14 1 46 21 0.0 6 17 3 0 1 47 24 0.0 6 22 1 1 48 17 0.0 6 16 0 0 0 0 0 1 49 32 0.0 6 30 2 50 34 0.0 6 31 3 51 18 0.0 6 15 3 52 34 0.0 6 32 2 53 21 0.0 6 20 0 1 54 30 0.0 6 27 3 55 35 0.0 6 33 2 56 32 0.0 6 29 3 57 25 0.0 6 23 2 58 34 0.0 6 29 5 59 40 0.0 6 35 5 60 30 0.0 6 27 3 61 28 0.0 6 27 1 62 28 0.0 6 27 1 63 35 0.0 6 31 4 64 37 0.0 6 35 2 65 39 0.0 6 37 1 0 1 66 35 0.0 6 31 3 1 67 61 0.0 6 52 9 68 189 0.0 6 181 7 0 0 0 1 69 64 0.0 6 60 2 0 1 1 70 82 0.0 6 77 2 1 0 0 0 2 71 43 0.0 6 40 3 72 67 0.0 6 64 2 1 73 38 0.0 6 37 1 74 47 0.0 6 47 75 59 0.0 6 58 0 0 1 76 68 0.0 6 63 5 77 65 0.0 6 61 3 0 1 78 59 0.0 6 57 2 79 44 0.0 6 44 80 74 0.0 6 72 2 81 66 0.0 6 61 3 1 1 82 76 0.0 6 73 3 83 90 0.0 6 87 3 84 102 0.0 6 99 2 0 0 0 1 85 56 0.0 6 54 2 86 72 0.0 6 67 5 87 59 0.0 6 53 6 88 81 0.0 6 76 5 89 62 0.0 6 61 1 90 77 0.0 6 73 2 2 91 82 0.0 6 78 3 1 92 78 0.0 6 76 2 93 84 0.0 6 82 2 94 117 0.0 6 110 5 1 1 95 120 0.0 6 116 3 0 0 0 0 1 96 95 0.0 6 89 5 0 0 0 1 97 135 0.0 6 131 3 0 1 98 106 0.0 6 102 3 1 99 104 0.0 6 103 1 100 101 0.0 6 97 4 101 110 0.0 6 103 7 102 101 0.0 6 97 4 103 137 0.0 6 130 7 104 170 0.0 6 162 7 1 105 262 0.0 6 254 5 2 0 1 106 164 0.0 6 161 3 107 129 0.0 6 127 1 1 108 153 0.0 6 146 5 0 1 1 109 164 0.0 6 153 11 110 159 0.0 6 155 4 111 179 0.0 6 174 5 112 194 0.0 6 192 2 113 227 0.0 6 217 9 0 0 0 1 114 204 0.0 6 196 6 0 0 1 0 1 115 201 0.0 6 196 5 116 250 0.0 6 246 3 1 117 214 0.0 6 206 7 1 118 283 0.0 6 274 8 0 0 0 0 1 119 281 0.0 6 277 4 120 228 0.0 6 223 5 121 264 0.0 6 260 4 122 261 0.0 6 255 4 1 1 123 278 0.0 6 268 8 0 1 0 0 1 124 350 0.0 6 338 7 0 1 1 2 1 125 385 0.0 6 380 5 126 421 0.0 6 415 6 127 414 0.0 6 404 6 1 1 0 1 1 128 496 0.0 6 488 7 0 0 1 129 467 0.0 6 459 5 0 1 1 0 1 130 568 0.0 6 550 14 0 0 1 0 3 131 550 0.0 6 536 12 1 0 0 1 132 557 0.0 6 544 9 2 0 0 0 2 133 614 0.0 6 606 7 1 134 643 0.0 6 633 7 0 1 0 1 1 135 710 0.0 6 700 7 1 0 0 1 1 136 637 0.0 6 624 11 0 0 0 1 1 137 641 0.0 6 636 5 138 702 0.0 6 692 9 1 139 875 0.0 6 860 11 0 1 3 140 1061 0.0 6 1050 11 141 1230 0.0 6 1218 8 2 0 1 1 142 1138 0.0 6 1122 11 0 0 2 1 2 143 1261 0.0 6 1247 10 1 0 1 2 144 1241 0.0 6 1230 9 1 0 0 0 1 145 1279 0.0 6 1261 16 0 2 146 1441 0.0 6 1424 14 1 1 0 1 147 1477 0.0 6 1465 9 1 1 0 1 148 1412 0.0 6 1397 11 1 0 1 1 1 149 1501 0.0 6 1487 12 1 0 1 150 1757 0.0 6 1738 18 1 151 2285 0.0 6 2251 24 1 3 3 1 2 152 1715 0.0 6 1694 15 1 2 2 1 153 2094 0.0 6 2075 14 1 1 0 0 3 154 1990 0.0 6 1967 21 1 0 0 0 1 155 1587 0.0 6 1571 16 156 1787 0.0 6 1775 7 0 2 1 1 1 157 1575 0.0 6 1561 13 0 1 158 1604 0.0 6 1588 12 0 0 1 1 2 159 1710 0.0 6 1697 12 0 0 0 1 160 1666 0.0 6 1660 5 0 0 0 0 1 161 1448 0.0 6 1438 7 2 0 0 0 1 162 1425 0.0 6 1412 12 0 0 0 1 163 1186 0.0 6 1175 9 0 0 1 1 164 1254 0.0 6 1248 4 0 2 165 1254 0.0 6 1248 6 166 1191 0.0 6 1180 9 0 0 1 1 167 1512 0.0 6 1503 8 0 0 0 0 1 168 1183 0.0 6 1179 3 0 1 169 1395 0.0 6 1384 7 1 0 0 2 1 170 1195 0.0 6 1185 9 0 0 0 1 171 1264 0.0 6 1255 6 0 1 1 0 1 172 1303 0.0 6 1297 3 0 1 0 1 1 173 1245 0.0 6 1239 4 1 0 1 174 1337 0.0 6 1333 4 175 1188 0.0 6 1178 6 2 1 0 1 176 1222 0.0 6 1211 9 1 0 0 1 177 1314 0.0 6 1306 5 0 0 1 2 178 890 0.0 6 886 3 0 0 0 1 179 918 0.0 6 913 4 1 180 728 0.0 6 726 2 181 679 0.0 6 678 0 0 0 0 1 182 655 0.0 6 649 5 1 183 645 0.0 6 644 1 184 963 0.0 6 955 8 185 1222 0.0 6 1216 6 186 981 0.0 6 977 3 0 0 1 187 659 0.0 6 656 3 188 622 0.0 6 620 2 189 554 0.0 6 550 3 0 0 1 190 546 0.0 6 542 4 191 455 0.0 6 454 1 192 471 0.0 6 468 2 1 193 343 0.0 6 341 2 194 311 0.0 6 310 0 1 195 304 0.0 6 302 2 196 253 0.0 6 251 2 197 327 0.0 6 326 1 198 257 0.0 6 257 199 223 0.0 6 221 0 1 1 200 198 0.0 6 197 0 0 0 0 1 201 182 0.0 6 180 1 1 202 165 0.0 6 164 1 203 152 0.0 6 150 1 1 204 124 0.0 6 123 1 205 128 0.0 6 128 206 111 0.0 6 110 0 1 207 103 0.0 6 103 208 105 0.0 6 105 209 83 0.0 6 83 210 71 0.0 6 70 0 0 1 211 64 0.0 6 64 212 54 0.0 6 54 213 67 0.0 6 66 1 214 60 0.0 6 59 0 0 1 215 93 0.0 6 93 216 66 0.0 6 63 1 0 1 0 1 217 46 0.0 6 46 218 39 0.0 6 39 219 42 0.0 6 42 220 26 0.0 6 26 221 16 0.0 6 16 222 32 0.0 6 32 223 20 0.0 6 20 224 15 0.0 6 15 225 14 0.0 6 14 226 15 0.0 6 15 227 9 0.0 6 8 1 228 10 0.0 6 10 229 8 0.0 6 8 230 10 0.0 6 10 231 3 0.0 6 3 232 12 0.0 6 11 1 233 3 0.0 6 3 234 4 0.0 6 4 235 3 0.0 6 3 236 1 0.0 6 1 237 2 0.0 6 2 238 1 0.0 6 1 239 1 0.0 6 1 240 2 0.0 6 2 242 6 0.0 6 6 243 2 0.0 6 2 244 2 0.0 6 2 248 1 0.0 6 1 249 2 0.0 6 2 250 1 0.0 6 1 251 1 0.0 6 1 277 1 0.0 6 1 278 2 0.0 6 2 279 6 0.0 6 6 280 55 0.0 6 55 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2006 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 6 85 21.9 1 0 0 85 51 1 0.0 6 1 52 1 0.0 6 1 58 2 0.0 6 2 60 1 0.0 6 1 63 1 0.0 6 1 68 2 0.0 6 2 69 1 0.0 6 1 70 5 0.0 6 5 71 12 0.0 6 12 72 17 0.0 6 17 73 28 0.0 6 28 74 5 0.0 6 5 75 15 0.0 6 15 76 19 0.0 6 19 77 7 0.0 6 7 78 16 0.0 6 16 79 19 0.0 6 17 0 0 2 80 14 0.0 6 14 81 21 0.0 6 21 82 6 0.0 6 6 83 10 0.0 6 10 84 12 0.0 6 12 85 14 0.0 6 14 86 17 0.0 6 17 87 8 0.0 6 8 88 11 0.0 6 11 89 10 0.0 6 10 90 9 0.0 6 9 91 27 0.0 6 27 92 12 0.0 6 11 0 0 0 1 93 12 0.0 6 12 94 12 0.0 6 12 95 8 0.0 6 8 96 9 0.0 6 9 97 13 0.0 6 13 98 15 0.0 6 15 99 8 0.0 6 8 100 10 0.0 6 10 101 10 0.0 6 9 0 0 1 102 13 0.0 6 13 103 14 0.0 6 14 104 11 0.0 6 11 105 11 0.0 6 11 106 15 0.0 6 15 107 8 0.0 6 8 108 21 0.0 6 21 109 58 0.0 6 57 1 110 12 0.0 6 12 111 10 0.0 6 10 112 14 0.0 6 13 1 113 6 0.0 6 6 114 7 0.0 6 7 115 12 0.0 6 12 116 13 0.0 6 12 0 0 1 117 11 0.0 6 11 118 14 0.0 6 14 119 10 0.0 6 10 120 10 0.0 6 10 121 14 0.0 6 14 122 16 0.0 6 14 0 1 0 1 123 13 0.0 6 13 124 19 0.0 6 18 0 0 0 1 125 21 0.0 6 21 126 19 0.0 6 19 127 24 0.0 6 24 128 15 0.0 6 15 129 13 0.0 6 13 130 15 0.0 6 15 131 21 0.0 6 21 132 14 0.0 6 14 133 43 0.0 6 43 134 15 0.0 6 15 135 19 0.0 6 19 136 13 0.0 6 13 137 17 0.0 6 17 138 27 0.0 6 27 139 22 0.0 6 22 140 16 0.0 6 16 141 22 0.0 6 22 142 21 0.0 6 20 0 0 0 1 143 20 0.0 6 20 144 24 0.0 6 24 145 18 0.0 6 17 1 146 19 0.0 6 19 147 21 0.0 6 20 0 0 1 148 13 0.0 6 13 149 18 0.0 6 18 150 16 0.0 6 15 0 0 0 1 151 27 0.0 6 27 152 14 0.0 6 14 153 17 0.0 6 17 154 26 0.0 6 26 155 7 0.0 6 7 156 15 0.0 6 15 157 31 0.0 6 31 158 16 0.0 6 16 159 16 0.0 6 16 160 14 0.0 6 14 161 17 0.0 6 17 162 23 0.0 6 23 163 27 0.0 6 27 164 10 0.0 6 10 165 16 0.0 6 16 166 21 0.0 6 21 167 15 0.0 6 15 168 20 0.0 6 19 0 0 0 1 169 16 0.0 6 16 170 15 0.0 6 15 171 19 0.0 6 19 172 17 0.0 6 17 173 17 0.0 6 17 174 12 0.0 6 12 175 12 0.0 6 12 176 4 0.0 6 4 177 7 0.0 6 7 178 10 0.0 6 10 179 7 0.0 6 6 0 1 180 5 0.0 6 5 181 14 0.0 6 14 182 14 0.0 6 14 183 11 0.0 6 11 184 14 0.0 6 14 185 4 0.0 6 4 186 8 0.0 6 8 187 8 0.0 6 8 188 13 0.0 6 13 189 8 0.0 6 8 190 11 0.0 6 11 191 5 0.0 6 5 192 3 0.0 6 3 193 6 0.0 6 6 194 2 0.0 6 2 195 5 0.0 6 5 196 5 0.0 6 5 197 4 0.0 6 4 198 1 0.0 6 1 199 10 0.0 6 9 0 0 0 1 200 3 0.0 6 3 201 1 0.0 6 1 202 6 0.0 6 6 203 5 0.0 6 4 0 0 0 1 204 3 0.0 6 3 205 3 0.0 6 3 206 2 0.0 6 2 207 2 0.0 6 2 208 2 0.0 6 2 209 1 0.0 6 1 211 2 0.0 6 2 212 1 0.0 6 1 213 1 0.0 6 1 215 1 0.0 6 1 217 1 0.0 6 1 218 1 0.0 6 1 219 1 0.0 6 1 222 1 0.0 6 1 225 1 0.0 6 1 227 1 0.0 6 1 228 1 0.0 6 1 234 2 0.0 6 2 238 1 0.0 6 1 239 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_09_159_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_09_386_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_09_159_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_09_386_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.83 s (38 µs/read; 1.59 M reads/minute). === Summary === Total read pairs processed: 101,586 Read 1 with adapter: 101,575 (100.0%) Read 2 with adapter: 101,560 (100.0%) == Read fate breakdown == Pairs that were too short: 52 (0.1%) Pairs discarded as untrimmed: 37 (0.0%) Pairs written (passing filters): 101,497 (99.9%) Total basepairs processed: 43,892,497 bp Read 1: 23,725,267 bp Read 2: 20,167,230 bp Total written (filtered): 6,479,490 bp (14.8%) Read 1: 3,375,348 bp Read 2: 3,104,142 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 97459 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1587.3 0 1 4 1 396.8 1 1 6 1 24.8 1 0 0 1 7 1 6.2 2 0 0 1 9 3 0.4 2 3 14 1 0.0 4 1 15 2 0.0 4 2 16 6 0.0 4 5 1 17 2 0.0 5 2 18 1 0.0 5 1 19 5 0.0 5 3 2 20 128 0.0 6 122 6 21 54 0.0 6 53 1 22 56 0.0 6 55 1 23 36 0.0 6 35 1 24 25 0.0 6 25 25 29 0.0 6 28 1 26 22 0.0 6 21 0 1 27 14 0.0 6 14 28 15 0.0 6 15 29 8 0.0 6 8 30 6 0.0 6 6 31 10 0.0 6 10 32 8 0.0 6 8 33 7 0.0 6 7 34 9 0.0 6 8 1 35 3 0.0 6 3 36 8 0.0 6 7 1 37 6 0.0 6 6 38 9 0.0 6 7 1 1 39 8 0.0 6 8 40 14 0.0 6 13 0 0 0 1 41 8 0.0 6 8 42 8 0.0 6 8 43 10 0.0 6 8 2 44 3 0.0 6 2 1 45 2 0.0 6 2 46 5 0.0 6 4 1 47 4 0.0 6 4 48 8 0.0 6 8 49 8 0.0 6 7 1 50 9 0.0 6 8 1 51 12 0.0 6 12 52 5 0.0 6 5 53 4 0.0 6 4 54 8 0.0 6 6 2 55 5 0.0 6 5 56 4 0.0 6 4 57 3 0.0 6 3 58 2 0.0 6 2 59 4 0.0 6 3 1 60 2 0.0 6 2 61 7 0.0 6 6 0 1 62 5 0.0 6 5 63 12 0.0 6 11 1 64 8 0.0 6 8 65 6 0.0 6 6 66 8 0.0 6 8 67 7 0.0 6 7 68 6 0.0 6 5 1 69 10 0.0 6 10 70 12 0.0 6 11 1 71 7 0.0 6 6 0 0 0 0 0 1 72 5 0.0 6 5 73 14 0.0 6 12 2 74 9 0.0 6 9 75 14 0.0 6 14 76 9 0.0 6 9 77 10 0.0 6 9 0 0 0 1 78 12 0.0 6 12 79 6 0.0 6 5 1 80 8 0.0 6 8 81 11 0.0 6 10 0 1 82 6 0.0 6 6 83 8 0.0 6 7 1 84 11 0.0 6 11 85 11 0.0 6 11 86 17 0.0 6 16 1 87 12 0.0 6 12 88 11 0.0 6 9 2 89 11 0.0 6 10 1 90 7 0.0 6 6 0 0 0 0 0 1 91 8 0.0 6 8 92 11 0.0 6 10 1 93 11 0.0 6 9 2 94 8 0.0 6 6 1 1 95 8 0.0 6 8 96 11 0.0 6 11 97 12 0.0 6 12 98 15 0.0 6 15 99 16 0.0 6 16 100 8 0.0 6 8 101 16 0.0 6 15 1 102 13 0.0 6 13 103 17 0.0 6 17 104 23 0.0 6 23 105 12 0.0 6 11 1 106 17 0.0 6 17 107 21 0.0 6 21 108 12 0.0 6 12 109 10 0.0 6 10 110 21 0.0 6 21 111 23 0.0 6 23 112 11 0.0 6 10 1 113 19 0.0 6 17 2 114 15 0.0 6 14 1 115 22 0.0 6 21 1 116 25 0.0 6 25 117 25 0.0 6 25 118 28 0.0 6 27 1 119 18 0.0 6 17 0 0 0 0 0 1 120 19 0.0 6 19 121 19 0.0 6 19 122 16 0.0 6 16 123 24 0.0 6 24 124 25 0.0 6 23 1 0 0 0 0 1 125 31 0.0 6 31 126 42 0.0 6 42 127 42 0.0 6 42 128 43 0.0 6 43 129 49 0.0 6 47 2 130 37 0.0 6 36 1 131 39 0.0 6 36 1 0 0 1 0 1 132 52 0.0 6 52 133 70 0.0 6 70 134 58 0.0 6 57 0 0 0 0 1 135 56 0.0 6 56 136 60 0.0 6 60 137 53 0.0 6 53 138 49 0.0 6 47 2 139 137 0.0 6 133 4 140 247 0.0 6 246 1 141 241 0.0 6 240 1 142 407 0.0 6 406 1 143 616 0.0 6 614 1 0 0 0 1 144 412 0.0 6 410 2 145 194 0.0 6 192 2 146 638 0.0 6 637 1 147 561 0.0 6 557 4 148 181 0.0 6 179 2 149 274 0.0 6 273 0 0 0 0 0 1 150 814 0.0 6 810 0 0 0 0 4 151 224 0.0 6 223 0 0 0 0 1 152 640 0.0 6 639 1 153 216 0.0 6 216 154 128 0.0 6 128 155 225 0.0 6 223 2 156 191 0.0 6 190 0 0 0 0 0 1 157 247 0.0 6 245 2 158 180 0.0 6 177 2 1 159 287 0.0 6 284 2 0 0 0 1 160 693 0.0 6 688 4 0 0 0 0 1 161 350 0.0 6 349 0 1 162 651 0.0 6 649 2 163 318 0.0 6 315 3 164 348 0.0 6 348 165 624 0.0 6 624 166 799 0.0 6 797 1 0 0 0 0 1 167 912 0.0 6 907 2 0 1 1 1 168 543 0.0 6 540 3 169 2152 0.0 6 2147 5 170 983 0.0 6 980 2 0 0 0 1 171 1028 0.0 6 1024 4 172 405 0.0 6 404 1 173 1484 0.0 6 1483 1 174 1195 0.0 6 1192 2 0 0 1 175 1180 0.0 6 1175 4 1 176 2274 0.0 6 2266 7 0 0 0 0 1 177 482 0.0 6 478 3 0 0 1 178 249 0.0 6 248 0 1 179 224 0.0 6 222 2 180 271 0.0 6 271 181 374 0.0 6 371 2 0 0 0 0 1 182 1839 0.0 6 1829 10 183 665 0.0 6 660 5 184 1710 0.0 6 1705 4 0 0 0 0 1 185 1400 0.0 6 1396 4 186 1546 0.0 6 1543 3 187 1307 0.0 6 1302 4 0 0 0 0 1 188 572 0.0 6 568 4 189 1466 0.0 6 1460 6 190 1372 0.0 6 1367 5 191 670 0.0 6 667 2 0 0 0 0 1 192 1657 0.0 6 1657 193 630 0.0 6 628 2 194 2292 0.0 6 2287 4 1 195 775 0.0 6 770 5 196 3059 0.0 6 3051 8 197 839 0.0 6 836 2 0 0 0 1 198 619 0.0 6 613 6 199 787 0.0 6 785 1 0 0 0 0 1 200 1481 0.0 6 1476 5 201 629 0.0 6 627 2 202 400 0.0 6 396 4 203 360 0.0 6 357 2 0 0 0 0 1 204 517 0.0 6 513 3 0 0 1 205 465 0.0 6 463 2 206 642 0.0 6 640 1 0 0 1 207 889 0.0 6 886 2 0 0 1 208 1017 0.0 6 1015 2 209 1578 0.0 6 1572 6 210 471 0.0 6 468 2 0 0 0 0 1 211 315 0.0 6 314 1 212 533 0.0 6 530 3 213 712 0.0 6 710 2 214 682 0.0 6 679 1 1 0 0 1 215 1164 0.0 6 1160 3 1 216 1114 0.0 6 1109 4 1 217 1122 0.0 6 1118 2 0 0 0 1 1 218 911 0.0 6 905 4 1 0 0 1 219 1142 0.0 6 1137 5 220 1676 0.0 6 1668 8 221 906 0.0 6 901 5 222 1468 0.0 6 1461 4 1 0 2 223 692 0.0 6 689 3 224 729 0.0 6 727 2 225 1335 0.0 6 1327 6 0 1 1 226 740 0.0 6 735 5 227 1017 0.0 6 1011 5 1 228 1236 0.0 6 1234 2 229 1564 0.0 6 1559 5 230 648 0.0 6 645 3 231 698 0.0 6 695 2 0 0 1 232 420 0.0 6 416 4 233 536 0.0 6 535 1 234 736 0.0 6 735 1 235 237 0.0 6 235 2 236 301 0.0 6 299 2 237 377 0.0 6 376 1 238 731 0.0 6 729 1 0 1 239 226 0.0 6 224 1 1 240 235 0.0 6 232 3 241 316 0.0 6 313 2 0 0 0 1 242 268 0.0 6 265 3 243 343 0.0 6 342 1 244 342 0.0 6 336 5 0 0 0 1 245 263 0.0 6 262 1 246 268 0.0 6 267 1 247 430 0.0 6 429 1 248 770 0.0 6 768 2 249 461 0.0 6 458 3 250 396 0.0 6 391 4 1 251 206 0.0 6 206 252 172 0.0 6 171 1 253 167 0.0 6 166 1 254 121 0.0 6 120 1 255 151 0.0 6 151 256 171 0.0 6 168 3 257 208 0.0 6 207 1 258 127 0.0 6 127 259 141 0.0 6 140 1 260 205 0.0 6 203 1 0 1 261 137 0.0 6 137 262 211 0.0 6 209 2 263 132 0.0 6 132 264 152 0.0 6 148 3 1 265 128 0.0 6 127 1 266 99 0.0 6 98 1 267 74 0.0 6 72 1 1 268 84 0.0 6 83 1 269 107 0.0 6 105 2 270 165 0.0 6 162 3 271 158 0.0 6 157 1 272 124 0.0 6 119 5 273 115 0.0 6 111 1 2 1 274 240 0.0 6 233 4 0 2 0 0 1 275 352 0.0 6 351 0 0 0 0 1 276 217 0.0 6 213 4 277 482 0.0 6 476 6 278 626 0.0 6 616 9 0 0 0 0 1 279 1635 0.0 6 1620 10 1 0 0 2 2 280 4070 0.0 6 4019 50 1 281 32 0.0 6 31 1 282 2 0.0 6 2 283 1 0.0 6 1 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 4116 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 6 0.0 6 6 72 9 0.0 6 9 73 27 0.0 6 27 74 27 0.0 6 27 75 13 0.0 6 13 76 12 0.0 6 12 77 34 0.0 6 34 78 24 0.0 6 24 79 5 0.0 6 5 80 22 0.0 6 22 81 134 0.0 6 133 0 0 0 0 0 1 82 8 0.0 6 8 83 30 0.0 6 30 84 4 0.0 6 4 85 5 0.0 6 5 86 5 0.0 6 5 87 5 0.0 6 5 88 3 0.0 6 3 89 4 0.0 6 4 90 10 0.0 6 10 91 32 0.0 6 32 92 15 0.0 6 15 93 22 0.0 6 22 94 6 0.0 6 6 95 15 0.0 6 15 96 39 0.0 6 39 97 40 0.0 6 39 1 98 42 0.0 6 42 99 37 0.0 6 37 100 109 0.0 6 109 101 45 0.0 6 45 102 35 0.0 6 35 103 11 0.0 6 11 104 76 0.0 6 74 1 0 0 0 0 1 105 64 0.0 6 64 106 55 0.0 6 55 107 90 0.0 6 90 108 12 0.0 6 12 109 3 0.0 6 3 110 1 0.0 6 1 111 3 0.0 6 3 112 31 0.0 6 31 113 70 0.0 6 69 1 114 30 0.0 6 30 115 70 0.0 6 70 116 60 0.0 6 60 117 64 0.0 6 64 118 37 0.0 6 37 119 24 0.0 6 24 120 79 0.0 6 79 121 39 0.0 6 39 122 17 0.0 6 17 123 55 0.0 6 54 1 124 41 0.0 6 41 125 89 0.0 6 88 1 126 46 0.0 6 46 127 142 0.0 6 142 128 9 0.0 6 9 129 2 0.0 6 2 130 19 0.0 6 19 131 67 0.0 6 67 132 6 0.0 6 6 133 11 0.0 6 11 134 4 0.0 6 4 135 1 0.0 6 1 136 1 0.0 6 1 137 2 0.0 6 2 138 15 0.0 6 15 139 41 0.0 6 40 0 0 0 0 0 1 140 85 0.0 6 85 141 5 0.0 6 5 142 1 0.0 6 1 144 3 0.0 6 3 145 2 0.0 6 2 146 5 0.0 6 5 147 1 0.0 6 1 148 3 0.0 6 3 149 2 0.0 6 2 150 35 0.0 6 35 151 91 0.0 6 91 152 37 0.0 6 36 1 153 107 0.0 6 107 154 8 0.0 6 8 155 40 0.0 6 40 156 122 0.0 6 122 157 9 0.0 6 9 159 1 0.0 6 1 160 14 0.0 6 14 161 61 0.0 6 61 162 53 0.0 6 53 163 3 0.0 6 3 164 40 0.0 6 40 165 90 0.0 6 90 166 4 0.0 6 4 167 2 0.0 6 2 168 18 0.0 6 18 169 50 0.0 6 50 170 5 0.0 6 5 171 6 0.0 6 6 172 2 0.0 6 2 173 1 0.0 6 1 174 1 0.0 6 1 176 2 0.0 6 2 177 3 0.0 6 3 178 25 0.0 6 25 179 74 0.0 6 74 180 41 0.0 6 41 181 50 0.0 6 50 182 5 0.0 6 5 187 1 0.0 6 1 189 1 0.0 6 1 190 3 0.0 6 3 191 2 0.0 6 2 192 76 0.0 6 76 193 170 0.0 6 170 194 11 0.0 6 9 2 195 1 0.0 6 1 196 2 0.0 6 2 197 1 0.0 6 1 198 1 0.0 6 0 0 0 0 0 0 1 200 2 0.0 6 2 201 6 0.0 6 6 202 26 0.0 6 26 203 1 0.0 6 1 211 3 0.0 6 3 212 6 0.0 6 6 213 67 0.0 6 67 214 118 0.0 6 118 215 6 0.0 6 5 1 216 11 0.0 6 11 217 41 0.0 6 40 1 218 4 0.0 6 4 219 1 0.0 6 0 0 0 0 0 0 1 220 9 0.0 6 9 221 47 0.0 6 47 222 3 0.0 6 3 223 3 0.0 6 3 224 10 0.0 6 10 228 1 0.0 6 1 229 1 0.0 6 1 235 1 0.0 6 1 237 4 0.0 6 4 238 8 0.0 6 8 239 1 0.0 6 1 241 1 0.0 6 1 244 1 0.0 6 1 245 1 0.0 6 1 246 1 0.0 6 1 247 1 0.0 6 1 248 2 0.0 6 2 249 3 0.0 6 3 250 21 0.0 6 21 251 86 0.0 6 86 252 3 0.0 6 3 267 1 0.0 6 1 269 2 0.0 6 2 277 2 0.0 6 2 278 3 0.0 6 3 279 1 0.0 6 1 280 1 0.0 6 1 281 1 0.0 6 1 300 1 0.0 6 1 302 1 0.0 6 1 307 3 0.0 6 3 308 5 0.0 6 5 309 4 0.0 6 4 310 4 0.0 6 4 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 97278 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 396.8 1 1 5 1 99.2 1 1 6 2 24.8 1 2 10 1 0.1 3 1 11 2 0.0 3 2 12 4 0.0 3 3 0 0 1 13 6 0.0 3 5 1 14 9 0.0 4 7 2 15 3 0.0 4 3 16 3 0.0 4 2 1 17 16 0.0 5 14 1 1 18 3 0.0 5 2 1 19 33 0.0 5 29 4 20 93 0.0 6 87 5 0 1 21 50 0.0 6 48 1 1 22 32 0.0 6 30 2 23 34 0.0 6 33 0 1 24 26 0.0 6 24 2 25 18 0.0 6 16 2 26 14 0.0 6 13 1 27 13 0.0 6 11 2 28 15 0.0 6 14 1 29 10 0.0 6 9 1 30 15 0.0 6 13 1 0 0 1 31 4 0.0 6 4 32 10 0.0 6 9 0 1 33 10 0.0 6 7 2 0 1 34 11 0.0 6 9 2 35 10 0.0 6 10 36 12 0.0 6 11 0 0 0 1 37 11 0.0 6 10 1 38 9 0.0 6 7 1 0 0 0 1 39 7 0.0 6 6 1 40 12 0.0 6 11 1 41 15 0.0 6 12 2 0 1 42 11 0.0 6 8 2 1 43 8 0.0 6 5 2 1 44 13 0.0 6 12 1 45 10 0.0 6 8 1 0 1 46 16 0.0 6 16 47 8 0.0 6 7 1 48 11 0.0 6 11 49 4 0.0 6 3 1 50 13 0.0 6 12 1 51 17 0.0 6 15 1 0 0 0 1 52 7 0.0 6 7 53 19 0.0 6 17 2 54 24 0.0 6 23 1 55 18 0.0 6 16 1 1 56 8 0.0 6 6 1 0 1 57 13 0.0 6 10 3 58 25 0.0 6 24 1 59 20 0.0 6 16 1 1 0 1 1 60 15 0.0 6 13 2 61 21 0.0 6 16 5 62 13 0.0 6 13 63 21 0.0 6 20 0 0 1 64 13 0.0 6 11 2 65 27 0.0 6 23 2 1 0 0 0 1 66 25 0.0 6 22 1 2 67 19 0.0 6 18 0 0 0 0 1 68 21 0.0 6 19 1 1 69 43 0.0 6 41 2 70 178 0.0 6 170 7 0 0 0 1 71 42 0.0 6 41 1 72 93 0.0 6 88 3 1 0 0 0 1 73 38 0.0 6 35 3 74 56 0.0 6 52 4 75 33 0.0 6 33 76 32 0.0 6 28 2 0 0 2 77 38 0.0 6 35 2 1 78 26 0.0 6 23 2 1 79 27 0.0 6 22 5 80 28 0.0 6 26 0 1 0 0 1 81 28 0.0 6 25 2 0 0 0 1 82 59 0.0 6 56 2 0 1 83 39 0.0 6 36 1 2 84 40 0.0 6 38 1 0 0 0 1 85 38 0.0 6 37 1 86 71 0.0 6 66 4 1 87 54 0.0 6 47 5 1 0 1 88 40 0.0 6 39 0 1 89 34 0.0 6 32 1 0 1 90 51 0.0 6 50 1 91 48 0.0 6 48 92 43 0.0 6 39 3 0 0 0 1 93 44 0.0 6 43 1 94 71 0.0 6 67 3 1 95 49 0.0 6 47 1 0 0 1 96 55 0.0 6 51 4 97 52 0.0 6 51 1 98 58 0.0 6 56 2 99 65 0.0 6 61 3 1 100 51 0.0 6 50 1 101 50 0.0 6 48 2 102 54 0.0 6 54 103 46 0.0 6 45 1 104 65 0.0 6 61 4 105 57 0.0 6 54 2 0 0 1 106 63 0.0 6 62 0 1 107 160 0.0 6 154 5 1 108 80 0.0 6 78 2 109 81 0.0 6 74 5 1 0 1 110 84 0.0 6 83 1 111 89 0.0 6 82 5 1 0 1 112 93 0.0 6 92 1 113 115 0.0 6 112 3 114 137 0.0 6 133 4 115 166 0.0 6 158 6 2 116 114 0.0 6 107 4 0 0 3 117 128 0.0 6 127 1 118 140 0.0 6 134 6 119 152 0.0 6 146 5 0 0 0 1 120 155 0.0 6 151 3 1 121 162 0.0 6 157 5 122 149 0.0 6 145 4 123 151 0.0 6 147 4 124 148 0.0 6 144 4 125 143 0.0 6 140 1 1 0 0 0 1 126 187 0.0 6 185 2 127 182 0.0 6 180 2 128 218 0.0 6 215 2 1 129 235 0.0 6 228 5 1 0 1 130 223 0.0 6 222 1 131 245 0.0 6 243 1 0 0 1 132 308 0.0 6 299 6 0 2 1 133 273 0.0 6 268 5 134 375 0.0 6 369 5 1 135 334 0.0 6 324 8 0 1 1 136 389 0.0 6 384 5 137 471 0.0 6 466 3 0 0 1 0 1 138 434 0.0 6 428 6 139 546 0.0 6 543 3 140 513 0.0 6 505 6 0 0 1 0 1 141 689 0.0 6 682 7 142 911 0.0 6 902 8 0 0 0 1 143 789 0.0 6 783 6 144 771 0.0 6 760 9 1 0 1 145 1029 0.0 6 1020 9 146 910 0.0 6 901 7 0 0 2 147 622 0.0 6 615 7 148 887 0.0 6 879 4 2 0 2 149 1428 0.0 6 1417 9 1 0 1 150 884 0.0 6 881 3 151 1356 0.0 6 1350 4 0 0 2 152 951 0.0 6 941 9 0 0 1 153 1301 0.0 6 1292 8 0 1 154 1122 0.0 6 1114 6 0 0 2 155 1468 0.0 6 1457 9 1 0 1 156 1254 0.0 6 1243 11 157 1390 0.0 6 1364 17 2 1 6 158 1312 0.0 6 1288 20 1 1 2 159 1409 0.0 6 1400 8 0 0 1 160 1323 0.0 6 1309 12 0 0 2 161 1639 0.0 6 1619 17 0 0 2 0 1 162 1496 0.0 6 1477 9 1 0 8 1 163 1401 0.0 6 1389 11 1 164 1657 0.0 6 1645 10 1 0 1 165 1633 0.0 6 1621 9 1 0 0 1 1 166 1552 0.0 6 1548 4 167 1486 0.0 6 1478 8 168 1998 0.0 6 1983 13 0 0 1 1 169 1833 0.0 6 1829 4 170 1825 0.0 6 1822 3 171 1518 0.0 6 1506 8 1 2 1 172 1735 0.0 6 1723 8 0 1 3 173 1595 0.0 6 1587 8 174 1597 0.0 6 1587 7 0 0 1 1 1 175 1802 0.0 6 1788 10 2 2 176 1598 0.0 6 1586 8 0 0 2 1 1 177 1195 0.0 6 1183 7 2 1 2 178 1526 0.0 6 1505 17 1 0 3 179 1730 0.0 6 1707 17 4 0 1 1 180 1032 0.0 6 1022 9 0 0 0 0 1 181 1672 0.0 6 1651 14 3 0 3 1 182 1129 0.0 6 1125 3 0 0 1 183 1121 0.0 6 1111 9 1 184 1094 0.0 6 1088 4 1 0 0 1 185 1085 0.0 6 1080 4 0 1 186 1911 0.0 6 1903 6 0 1 0 1 187 1418 0.0 6 1398 19 1 188 1692 0.0 6 1681 11 189 1098 0.0 6 1089 6 2 1 190 1333 0.0 6 1317 11 2 0 2 0 1 191 1178 0.0 6 1172 5 1 192 987 0.0 6 982 4 1 193 884 0.0 6 879 3 1 0 0 1 194 834 0.0 6 824 8 0 0 2 195 841 0.0 6 835 6 196 735 0.0 6 727 7 0 1 197 681 0.0 6 673 6 1 0 1 198 719 0.0 6 709 10 199 822 0.0 6 814 7 0 1 200 632 0.0 6 629 3 201 589 0.0 6 583 6 202 613 0.0 6 609 2 2 203 600 0.0 6 596 3 0 0 1 204 444 0.0 6 441 3 205 491 0.0 6 488 3 206 470 0.0 6 464 4 2 207 675 0.0 6 667 7 1 208 328 0.0 6 322 5 0 0 0 1 209 509 0.0 6 499 8 2 210 414 0.0 6 412 1 1 211 359 0.0 6 353 5 1 212 237 0.0 6 233 3 0 1 213 383 0.0 6 379 3 1 214 292 0.0 6 284 8 215 592 0.0 6 579 10 2 0 0 0 1 216 349 0.0 6 344 2 3 217 373 0.0 6 362 10 1 218 323 0.0 6 317 4 2 219 340 0.0 6 337 3 220 411 0.0 6 406 4 0 0 1 221 270 0.0 6 266 4 222 116 0.0 6 112 3 0 0 1 223 196 0.0 6 195 1 224 145 0.0 6 144 1 225 136 0.0 6 134 2 226 112 0.0 6 109 3 227 104 0.0 6 104 228 130 0.0 6 129 1 229 102 0.0 6 102 230 137 0.0 6 137 231 88 0.0 6 87 0 0 1 232 66 0.0 6 66 233 51 0.0 6 50 1 234 46 0.0 6 45 1 235 115 0.0 6 113 2 236 75 0.0 6 72 3 237 88 0.0 6 86 2 238 32 0.0 6 32 239 14 0.0 6 13 1 240 45 0.0 6 43 1 0 1 241 10 0.0 6 9 1 242 15 0.0 6 15 243 32 0.0 6 32 244 86 0.0 6 85 1 245 17 0.0 6 16 1 246 21 0.0 6 18 1 1 0 0 1 247 25 0.0 6 25 248 33 0.0 6 33 249 10 0.0 6 10 250 14 0.0 6 13 1 251 15 0.0 6 14 1 252 4 0.0 6 4 253 25 0.0 6 24 1 254 7 0.0 6 7 255 10 0.0 6 9 1 256 25 0.0 6 25 257 8 0.0 6 8 258 9 0.0 6 9 259 1 0.0 6 1 261 3 0.0 6 3 264 1 0.0 6 1 278 2 0.0 6 2 280 2 0.0 6 2 281 29 0.0 6 29 282 216 0.0 6 214 2 283 3 0.0 6 3 284 1 0.0 6 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 4282 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 68 84 0.0 6 83 1 69 42 0.0 6 41 1 70 11 0.0 6 11 71 23 0.0 6 23 72 22 0.0 6 22 73 15 0.0 6 15 74 6 0.0 6 6 75 53 0.0 6 53 76 16 0.0 6 16 77 3 0.0 6 3 78 15 0.0 6 15 79 63 0.0 6 63 80 6 0.0 6 6 81 28 0.0 6 28 82 1 0.0 6 1 83 4 0.0 6 4 84 10 0.0 6 10 85 4 0.0 6 4 86 12 0.0 6 12 87 4 0.0 6 4 88 16 0.0 6 16 89 34 0.0 6 34 90 6 0.0 6 6 91 42 0.0 6 42 92 1 0.0 6 1 93 16 0.0 6 14 0 0 0 2 94 34 0.0 6 34 95 40 0.0 6 39 0 0 0 1 96 38 0.0 6 38 97 31 0.0 6 31 98 145 0.0 6 145 99 38 0.0 6 38 100 60 0.0 6 60 101 14 0.0 6 13 0 0 1 102 71 0.0 6 70 0 0 0 1 103 67 0.0 6 67 104 58 0.0 6 58 105 103 0.0 6 103 106 11 0.0 6 10 0 0 0 1 107 4 0.0 6 4 108 3 0.0 6 2 0 0 0 0 1 109 5 0.0 6 5 110 38 0.0 6 38 111 101 0.0 6 98 3 112 101 0.0 6 99 2 113 86 0.0 6 86 114 57 0.0 6 57 115 71 0.0 6 70 0 0 1 116 42 0.0 6 42 117 14 0.0 6 11 0 0 0 2 0 1 118 73 0.0 6 73 119 56 0.0 6 56 120 8 0.0 6 8 121 55 0.0 6 55 122 29 0.0 6 28 0 0 0 1 123 135 0.0 6 135 124 36 0.0 6 36 125 194 0.0 6 194 126 4 0.0 6 3 0 0 0 1 127 4 0.0 6 2 0 0 0 2 128 16 0.0 6 15 0 0 1 129 74 0.0 6 73 0 0 0 1 130 5 0.0 6 5 131 6 0.0 6 6 132 7 0.0 6 6 1 133 51 0.0 6 48 3 135 4 0.0 6 4 136 18 0.0 6 18 137 43 0.0 6 43 138 118 0.0 6 117 1 139 3 0.0 6 3 141 1 0.0 6 0 0 0 1 143 1 0.0 6 1 144 11 0.0 6 11 145 2 0.0 6 2 146 3 0.0 6 3 147 2 0.0 6 2 148 18 0.0 6 18 149 110 0.0 6 110 150 21 0.0 6 21 151 166 0.0 6 166 152 5 0.0 6 5 153 39 0.0 6 39 154 164 0.0 6 164 155 2 0.0 6 1 0 0 0 1 156 1 0.0 6 1 158 4 0.0 6 4 159 72 0.0 6 72 160 66 0.0 6 66 161 3 0.0 6 2 0 0 1 162 37 0.0 6 37 163 113 0.0 6 112 1 164 2 0.0 6 2 166 11 0.0 6 11 167 59 0.0 6 57 0 0 0 2 168 4 0.0 6 4 169 5 0.0 6 5 170 1 0.0 6 1 171 4 0.0 6 4 173 1 0.0 6 0 0 0 1 174 2 0.0 6 2 176 22 0.0 6 22 177 103 0.0 6 103 178 27 0.0 6 26 1 179 55 0.0 6 55 180 5 0.0 6 5 184 2 0.0 6 2 185 1 0.0 6 1 187 1 0.0 6 1 188 1 0.0 6 1 189 8 0.0 6 7 1 190 44 0.0 6 44 191 182 0.0 6 182 192 7 0.0 6 7 194 5 0.0 6 5 199 4 0.0 6 4 200 24 0.0 6 23 1 210 1 0.0 6 1 211 13 0.0 6 13 212 55 0.0 6 55 213 3 0.0 6 3 214 1 0.0 6 1 215 24 0.0 6 24 216 2 0.0 6 2 218 3 0.0 6 3 219 17 0.0 6 17 221 1 0.0 6 1 222 2 0.0 6 2 246 1 0.0 6 1 247 1 0.0 6 1 248 5 0.0 6 4 1 249 22 0.0 6 22 250 1 0.0 6 0 1 258 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_10_160_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_10_387_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_10_160_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_10_387_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.49 s (75 µs/read; 0.81 M reads/minute). === Summary === Total read pairs processed: 33,379 Read 1 with adapter: 33,375 (100.0%) Read 2 with adapter: 33,357 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 26 (0.1%) Pairs written (passing filters): 33,353 (99.9%) Total basepairs processed: 16,252,297 bp Read 1: 9,252,579 bp Read 2: 6,999,718 bp Total written (filtered): 2,106,697 bp (13.0%) Read 1: 1,037,131 bp Read 2: 1,069,566 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 33318 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 4 8.1 1 0 0 4 14 1 0.0 4 1 15 2 0.0 4 2 16 1 0.0 4 1 20 9 0.0 6 8 1 21 6 0.0 6 6 23 1 0.0 6 1 25 5 0.0 6 5 26 2 0.0 6 2 28 1 0.0 6 1 29 1 0.0 6 1 32 1 0.0 6 1 33 4 0.0 6 4 34 2 0.0 6 2 35 2 0.0 6 2 37 1 0.0 6 1 39 1 0.0 6 1 40 2 0.0 6 2 41 1 0.0 6 1 42 1 0.0 6 1 43 1 0.0 6 1 44 2 0.0 6 2 45 3 0.0 6 3 46 1 0.0 6 1 47 1 0.0 6 1 48 1 0.0 6 1 49 2 0.0 6 1 1 50 4 0.0 6 3 1 53 1 0.0 6 1 54 4 0.0 6 4 56 4 0.0 6 4 57 3 0.0 6 3 58 1 0.0 6 1 60 3 0.0 6 2 0 1 63 1 0.0 6 1 64 1 0.0 6 1 67 1 0.0 6 0 1 68 2 0.0 6 2 69 1 0.0 6 1 72 1 0.0 6 1 73 1 0.0 6 1 74 1 0.0 6 1 75 1 0.0 6 1 76 2 0.0 6 2 77 2 0.0 6 2 80 1 0.0 6 1 82 2 0.0 6 2 84 1 0.0 6 1 86 5 0.0 6 5 87 3 0.0 6 3 89 6 0.0 6 6 90 2 0.0 6 2 91 3 0.0 6 3 92 3 0.0 6 3 93 1 0.0 6 1 97 2 0.0 6 2 98 6 0.0 6 6 99 3 0.0 6 3 100 3 0.0 6 3 101 6 0.0 6 6 104 3 0.0 6 3 105 1 0.0 6 1 106 2 0.0 6 2 107 8 0.0 6 8 108 9 0.0 6 8 1 109 3 0.0 6 3 110 2 0.0 6 2 111 6 0.0 6 5 1 112 3 0.0 6 3 113 4 0.0 6 4 114 2 0.0 6 2 115 1 0.0 6 0 1 116 4 0.0 6 4 117 2 0.0 6 2 119 6 0.0 6 5 1 120 7 0.0 6 7 121 4 0.0 6 4 122 6 0.0 6 5 1 123 4 0.0 6 4 124 5 0.0 6 5 125 7 0.0 6 6 1 126 19 0.0 6 18 1 127 13 0.0 6 12 1 128 12 0.0 6 10 2 129 3 0.0 6 3 130 9 0.0 6 9 131 11 0.0 6 11 132 11 0.0 6 11 133 12 0.0 6 12 134 11 0.0 6 11 135 18 0.0 6 17 1 136 12 0.0 6 10 2 137 14 0.0 6 14 138 12 0.0 6 12 139 8 0.0 6 8 140 18 0.0 6 17 1 141 23 0.0 6 21 2 142 17 0.0 6 17 143 26 0.0 6 26 144 36 0.0 6 35 0 1 145 44 0.0 6 44 146 62 0.0 6 61 1 147 76 0.0 6 76 148 71 0.0 6 69 1 1 149 78 0.0 6 78 150 81 0.0 6 81 151 50 0.0 6 49 1 152 66 0.0 6 66 153 57 0.0 6 57 154 50 0.0 6 50 155 53 0.0 6 52 1 156 51 0.0 6 51 157 39 0.0 6 39 158 37 0.0 6 37 159 54 0.0 6 54 160 37 0.0 6 37 161 55 0.0 6 54 1 162 70 0.0 6 69 1 163 33 0.0 6 33 164 55 0.0 6 54 1 165 37 0.0 6 35 2 166 21 0.0 6 21 167 48 0.0 6 48 168 38 0.0 6 38 169 45 0.0 6 45 170 73 0.0 6 72 1 171 52 0.0 6 48 3 1 172 28 0.0 6 28 173 76 0.0 6 75 1 174 48 0.0 6 47 1 175 78 0.0 6 78 176 37 0.0 6 37 177 56 0.0 6 54 2 178 50 0.0 6 50 179 34 0.0 6 34 180 49 0.0 6 48 1 181 70 0.0 6 69 1 182 69 0.0 6 68 1 183 79 0.0 6 72 7 184 80 0.0 6 77 3 185 82 0.0 6 81 1 186 78 0.0 6 78 187 58 0.0 6 56 2 188 79 0.0 6 78 1 189 75 0.0 6 73 2 190 66 0.0 6 63 3 191 81 0.0 6 79 2 192 75 0.0 6 75 193 92 0.0 6 90 2 194 92 0.0 6 90 2 195 90 0.0 6 88 2 196 110 0.0 6 109 1 197 127 0.0 6 121 6 198 146 0.0 6 145 1 199 168 0.0 6 164 4 200 135 0.0 6 132 2 1 201 160 0.0 6 154 6 202 125 0.0 6 123 2 203 129 0.0 6 126 2 0 1 204 117 0.0 6 113 4 205 147 0.0 6 145 2 206 159 0.0 6 156 3 207 179 0.0 6 174 5 208 132 0.0 6 129 3 209 126 0.0 6 124 2 210 94 0.0 6 93 1 211 93 0.0 6 90 3 212 106 0.0 6 104 2 213 109 0.0 6 108 0 0 1 214 154 0.0 6 150 4 215 201 0.0 6 196 5 216 172 0.0 6 166 6 217 193 0.0 6 191 2 218 133 0.0 6 129 4 219 136 0.0 6 129 7 220 188 0.0 6 187 1 221 112 0.0 6 111 1 222 100 0.0 6 98 2 223 174 0.0 6 167 7 224 274 0.0 6 273 1 225 189 0.0 6 188 1 226 252 0.0 6 246 6 227 213 0.0 6 206 7 228 211 0.0 6 209 2 229 136 0.0 6 131 5 230 198 0.0 6 193 5 231 248 0.0 6 242 6 232 190 0.0 6 184 6 233 196 0.0 6 191 5 234 208 0.0 6 203 5 235 178 0.0 6 177 1 236 197 0.0 6 195 2 237 170 0.0 6 166 4 238 285 0.0 6 278 7 239 359 0.0 6 353 6 240 304 0.0 6 299 5 241 348 0.0 6 341 7 242 248 0.0 6 242 6 243 379 0.0 6 376 3 244 156 0.0 6 154 2 245 259 0.0 6 254 5 246 191 0.0 6 186 4 1 247 265 0.0 6 257 8 248 178 0.0 6 173 5 249 276 0.0 6 268 7 1 250 210 0.0 6 203 7 251 219 0.0 6 215 4 252 328 0.0 6 323 5 253 211 0.0 6 207 4 254 287 0.0 6 277 10 255 206 0.0 6 202 3 0 1 256 256 0.0 6 250 4 1 0 1 257 422 0.0 6 411 11 258 436 0.0 6 429 7 259 281 0.0 6 274 7 260 460 0.0 6 446 14 261 552 0.0 6 546 6 262 264 0.0 6 259 5 263 348 0.0 6 342 6 264 136 0.0 6 135 1 265 248 0.0 6 238 10 266 328 0.0 6 316 12 267 409 0.0 6 406 3 268 211 0.0 6 201 10 269 549 0.0 6 537 11 1 270 1076 0.0 6 1061 15 271 333 0.0 6 323 10 272 528 0.0 6 521 6 1 273 536 0.0 6 522 14 274 695 0.0 6 683 12 275 755 0.0 6 738 17 276 503 0.0 6 492 11 277 619 0.0 6 606 13 278 757 0.0 6 728 28 0 1 279 4584 0.0 6 4481 97 3 1 1 0 1 280 2943 0.0 6 2869 70 4 281 22 0.0 6 20 2 282 2 0.0 6 2 283 1 0.0 6 0 0 0 0 1 284 1 0.0 6 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 57 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 73 1 0.0 6 1 75 4 0.0 6 4 76 4 0.0 6 4 77 4 0.0 6 4 78 2 0.0 6 2 79 2 0.0 6 2 80 1 0.0 6 1 84 3 0.0 6 3 86 1 0.0 6 1 87 1 0.0 6 1 88 2 0.0 6 2 90 1 0.0 6 1 92 1 0.0 6 1 93 1 0.0 6 1 99 1 0.0 6 1 102 1 0.0 6 1 104 1 0.0 6 1 111 1 0.0 6 1 115 1 0.0 6 1 116 1 0.0 6 1 125 1 0.0 6 1 132 1 0.0 6 1 134 1 0.0 6 1 135 1 0.0 6 1 137 1 0.0 6 1 142 1 0.0 6 1 150 1 0.0 6 1 151 1 0.0 6 1 152 2 0.0 6 2 156 1 0.0 6 1 169 1 0.0 6 1 175 1 0.0 6 1 178 1 0.0 6 1 191 1 0.0 6 0 0 0 0 0 0 1 202 1 0.0 6 1 209 2 0.0 6 2 210 1 0.0 6 1 213 2 0.0 6 2 269 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 33291 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 521.5 0 0 1 10 3 0.0 3 3 11 3 0.0 3 3 13 4 0.0 3 3 1 14 6 0.0 4 4 2 16 7 0.0 4 6 1 17 5 0.0 5 4 1 18 7 0.0 5 5 2 19 4 0.0 5 2 2 20 27 0.0 6 26 1 21 24 0.0 6 24 22 15 0.0 6 13 2 23 16 0.0 6 16 24 14 0.0 6 14 25 11 0.0 6 11 26 16 0.0 6 13 2 1 27 11 0.0 6 11 28 5 0.0 6 5 29 10 0.0 6 8 2 30 16 0.0 6 13 3 31 12 0.0 6 10 0 1 0 1 32 7 0.0 6 5 2 33 6 0.0 6 5 1 34 4 0.0 6 4 35 6 0.0 6 5 1 36 4 0.0 6 4 37 2 0.0 6 2 38 5 0.0 6 5 39 4 0.0 6 2 2 40 5 0.0 6 5 41 5 0.0 6 4 1 42 3 0.0 6 3 43 5 0.0 6 4 1 44 16 0.0 6 14 2 45 2 0.0 6 2 46 10 0.0 6 10 47 6 0.0 6 5 0 1 48 8 0.0 6 7 1 49 7 0.0 6 6 1 50 9 0.0 6 8 0 1 51 4 0.0 6 4 52 15 0.0 6 15 53 15 0.0 6 13 2 54 18 0.0 6 17 1 55 16 0.0 6 15 1 56 7 0.0 6 6 1 57 17 0.0 6 15 1 0 1 58 1 0.0 6 1 59 4 0.0 6 4 60 10 0.0 6 7 3 61 5 0.0 6 5 62 4 0.0 6 4 63 7 0.0 6 5 2 64 8 0.0 6 8 65 2 0.0 6 1 1 66 14 0.0 6 12 2 67 69 0.0 6 68 1 68 16 0.0 6 15 1 69 25 0.0 6 24 1 70 11 0.0 6 11 71 19 0.0 6 16 3 72 13 0.0 6 13 73 4 0.0 6 4 75 8 0.0 6 7 1 76 12 0.0 6 10 2 77 12 0.0 6 11 1 78 10 0.0 6 9 1 79 18 0.0 6 16 2 80 15 0.0 6 14 1 81 9 0.0 6 8 1 82 27 0.0 6 25 2 83 30 0.0 6 25 5 84 16 0.0 6 15 1 85 15 0.0 6 13 2 86 17 0.0 6 14 3 87 31 0.0 6 27 3 1 88 15 0.0 6 11 3 0 1 89 16 0.0 6 14 2 90 18 0.0 6 15 3 91 23 0.0 6 20 2 1 92 21 0.0 6 18 3 93 36 0.0 6 31 5 94 29 0.0 6 26 3 95 25 0.0 6 22 1 1 1 96 27 0.0 6 25 2 97 27 0.0 6 26 1 98 29 0.0 6 27 2 99 35 0.0 6 32 3 100 34 0.0 6 32 2 101 34 0.0 6 30 4 102 33 0.0 6 29 1 3 103 47 0.0 6 44 3 104 62 0.0 6 58 3 1 105 39 0.0 6 36 3 106 31 0.0 6 31 107 28 0.0 6 26 1 1 108 23 0.0 6 23 109 30 0.0 6 26 4 110 25 0.0 6 24 0 1 111 32 0.0 6 26 5 1 112 33 0.0 6 32 1 113 22 0.0 6 20 2 114 43 0.0 6 42 1 115 35 0.0 6 34 1 116 47 0.0 6 44 3 117 21 0.0 6 21 118 37 0.0 6 36 1 119 47 0.0 6 43 4 120 60 0.0 6 56 3 1 121 56 0.0 6 49 7 122 50 0.0 6 47 2 1 123 58 0.0 6 56 0 1 0 1 124 58 0.0 6 58 125 62 0.0 6 60 2 126 58 0.0 6 57 1 127 56 0.0 6 53 3 128 55 0.0 6 54 1 129 48 0.0 6 45 3 130 56 0.0 6 54 2 131 100 0.0 6 96 4 132 134 0.0 6 126 7 1 133 125 0.0 6 120 5 134 162 0.0 6 151 8 2 0 1 135 224 0.0 6 216 8 136 85 0.0 6 81 4 137 143 0.0 6 137 6 138 86 0.0 6 84 2 139 139 0.0 6 132 5 1 1 140 194 0.0 6 187 6 1 141 212 0.0 6 204 7 1 142 252 0.0 6 247 4 0 0 1 143 223 0.0 6 215 6 0 0 2 144 330 0.0 6 321 9 145 168 0.0 6 163 5 146 189 0.0 6 183 6 147 142 0.0 6 138 2 2 148 205 0.0 6 201 4 149 301 0.0 6 291 10 150 506 0.0 6 493 13 151 165 0.0 6 157 7 0 1 152 477 0.0 6 467 10 153 240 0.0 6 234 6 154 320 0.0 6 308 10 1 1 155 236 0.0 6 229 6 0 0 1 156 226 0.0 6 219 6 0 0 0 1 157 351 0.0 6 343 7 1 158 318 0.0 6 313 5 159 152 0.0 6 148 3 0 1 160 245 0.0 6 239 5 1 161 160 0.0 6 155 5 162 141 0.0 6 135 6 163 193 0.0 6 184 9 164 203 0.0 6 197 6 165 233 0.0 6 228 5 166 264 0.0 6 258 5 0 0 1 167 158 0.0 6 155 3 168 416 0.0 6 402 14 169 149 0.0 6 146 2 0 0 1 170 156 0.0 6 150 6 171 397 0.0 6 385 11 0 0 1 172 161 0.0 6 157 4 173 226 0.0 6 218 7 0 0 1 174 369 0.0 6 359 10 175 502 0.0 6 480 20 1 0 1 176 329 0.0 6 315 12 1 1 177 327 0.0 6 320 7 178 400 0.0 6 387 12 0 0 1 179 340 0.0 6 323 13 1 1 1 1 180 273 0.0 6 264 9 181 359 0.0 6 350 8 0 0 1 182 84 0.0 6 78 6 183 314 0.0 6 307 6 0 0 1 184 275 0.0 6 266 9 185 364 0.0 6 345 18 0 0 1 186 656 0.0 6 634 22 187 614 0.0 6 603 11 188 621 0.0 6 604 16 0 1 189 605 0.0 6 578 24 2 1 190 860 0.0 6 843 17 191 786 0.0 6 763 23 192 332 0.0 6 322 10 193 294 0.0 6 285 9 194 546 0.0 6 533 11 1 0 1 195 771 0.0 6 742 29 196 1210 0.0 6 1185 23 0 0 1 0 1 197 363 0.0 6 347 13 3 198 700 0.0 6 681 19 199 1139 0.0 6 1111 28 200 548 0.0 6 535 13 201 634 0.0 6 619 14 1 202 416 0.0 6 404 11 1 203 349 0.0 6 343 6 204 369 0.0 6 364 5 205 144 0.0 6 142 2 206 140 0.0 6 134 6 207 77 0.0 6 76 1 208 171 0.0 6 169 2 209 136 0.0 6 130 6 210 130 0.0 6 127 2 1 211 114 0.0 6 110 4 212 174 0.0 6 173 0 0 0 1 213 108 0.0 6 107 1 214 386 0.0 6 376 9 0 0 1 215 306 0.0 6 291 15 216 513 0.0 6 507 6 217 155 0.0 6 147 7 0 0 1 218 226 0.0 6 219 7 219 173 0.0 6 169 4 220 74 0.0 6 72 2 221 342 0.0 6 338 4 222 215 0.0 6 213 2 223 279 0.0 6 273 6 224 108 0.0 6 103 3 0 1 1 225 143 0.0 6 141 1 0 0 0 1 226 91 0.0 6 87 4 227 129 0.0 6 126 3 228 64 0.0 6 60 3 0 1 229 131 0.0 6 130 1 230 65 0.0 6 65 231 101 0.0 6 99 1 0 0 1 232 142 0.0 6 139 3 233 99 0.0 6 99 234 57 0.0 6 55 2 235 28 0.0 6 28 236 7 0.0 6 7 237 11 0.0 6 11 238 16 0.0 6 16 239 11 0.0 6 11 240 41 0.0 6 39 2 241 83 0.0 6 82 1 242 65 0.0 6 62 3 243 63 0.0 6 60 2 0 0 1 244 49 0.0 6 47 2 245 27 0.0 6 25 2 246 41 0.0 6 41 247 15 0.0 6 14 1 248 33 0.0 6 33 249 11 0.0 6 11 250 8 0.0 6 7 1 251 14 0.0 6 14 253 1 0.0 6 1 255 1 0.0 6 1 277 2 0.0 6 2 278 19 0.0 6 19 279 20 0.0 6 20 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 66 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 4 0.0 6 4 69 1 0.0 6 1 72 1 0.0 6 1 74 1 0.0 6 1 75 1 0.0 6 1 76 3 0.0 6 3 77 3 0.0 6 3 79 3 0.0 6 3 81 1 0.0 6 1 82 1 0.0 6 1 85 1 0.0 6 1 86 1 0.0 6 1 88 1 0.0 6 1 90 1 0.0 6 1 91 2 0.0 6 2 92 1 0.0 6 1 93 1 0.0 6 1 96 1 0.0 6 1 99 1 0.0 6 1 104 3 0.0 6 3 105 1 0.0 6 1 106 1 0.0 6 1 107 1 0.0 6 1 109 1 0.0 6 1 110 3 0.0 6 3 111 1 0.0 6 1 120 2 0.0 6 2 121 1 0.0 6 1 125 1 0.0 6 1 126 1 0.0 6 1 127 3 0.0 6 3 128 2 0.0 6 2 130 1 0.0 6 1 132 1 0.0 6 1 133 1 0.0 6 1 135 1 0.0 6 1 137 2 0.0 6 2 141 1 0.0 6 1 152 1 0.0 6 1 162 1 0.0 6 1 170 2 0.0 6 2 175 1 0.0 6 1 184 1 0.0 6 1 190 1 0.0 6 1 192 1 0.0 6 1 205 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_11_161_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_11_388_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_11_161_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_11_388_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.51 s (64 µs/read; 0.94 M reads/minute). === Summary === Total read pairs processed: 39,441 Read 1 with adapter: 39,437 (100.0%) Read 2 with adapter: 39,429 (100.0%) == Read fate breakdown == Pairs that were too short: 13 (0.0%) Pairs discarded as untrimmed: 16 (0.0%) Pairs written (passing filters): 39,412 (99.9%) Total basepairs processed: 17,063,612 bp Read 1: 9,114,221 bp Read 2: 7,949,391 bp Total written (filtered): 2,486,470 bp (14.6%) Read 1: 1,291,959 bp Read 2: 1,194,511 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 38155 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 616.3 0 1 6 1 9.6 1 0 0 1 7 1 2.4 2 1 9 1 0.2 2 0 1 10 2 0.0 3 2 11 1 0.0 3 1 17 1 0.0 5 1 19 1 0.0 5 1 20 40 0.0 6 39 1 21 19 0.0 6 18 1 22 16 0.0 6 16 23 14 0.0 6 14 24 16 0.0 6 14 2 25 8 0.0 6 8 26 9 0.0 6 8 1 27 10 0.0 6 9 1 28 7 0.0 6 7 29 2 0.0 6 1 1 30 5 0.0 6 5 31 3 0.0 6 2 1 32 7 0.0 6 7 33 4 0.0 6 3 1 34 5 0.0 6 5 36 2 0.0 6 2 37 3 0.0 6 3 38 2 0.0 6 2 39 5 0.0 6 5 40 1 0.0 6 1 41 4 0.0 6 4 42 3 0.0 6 3 43 5 0.0 6 5 44 2 0.0 6 2 45 2 0.0 6 2 46 5 0.0 6 4 1 47 1 0.0 6 1 48 1 0.0 6 1 49 4 0.0 6 3 1 50 1 0.0 6 1 51 5 0.0 6 5 52 3 0.0 6 3 54 3 0.0 6 3 56 2 0.0 6 1 0 1 57 2 0.0 6 1 1 58 1 0.0 6 1 59 3 0.0 6 3 60 1 0.0 6 1 61 1 0.0 6 1 62 3 0.0 6 3 63 2 0.0 6 2 64 4 0.0 6 4 65 3 0.0 6 3 66 2 0.0 6 2 67 4 0.0 6 4 68 4 0.0 6 4 70 1 0.0 6 1 71 1 0.0 6 1 72 7 0.0 6 7 73 2 0.0 6 2 74 6 0.0 6 5 0 0 1 75 1 0.0 6 1 76 5 0.0 6 5 77 2 0.0 6 2 78 8 0.0 6 8 80 4 0.0 6 2 2 81 8 0.0 6 8 82 1 0.0 6 1 83 5 0.0 6 5 84 9 0.0 6 9 85 4 0.0 6 4 86 1 0.0 6 1 87 3 0.0 6 3 88 2 0.0 6 2 89 6 0.0 6 5 1 90 4 0.0 6 4 91 3 0.0 6 3 92 1 0.0 6 1 93 4 0.0 6 4 94 1 0.0 6 1 95 1 0.0 6 1 96 1 0.0 6 1 97 5 0.0 6 5 98 1 0.0 6 1 99 6 0.0 6 6 100 7 0.0 6 7 101 2 0.0 6 2 102 7 0.0 6 7 103 6 0.0 6 6 104 6 0.0 6 6 105 11 0.0 6 11 106 9 0.0 6 9 107 5 0.0 6 5 108 7 0.0 6 7 109 10 0.0 6 10 110 2 0.0 6 2 111 14 0.0 6 14 112 18 0.0 6 18 113 13 0.0 6 13 114 5 0.0 6 5 115 5 0.0 6 4 1 116 6 0.0 6 6 117 10 0.0 6 10 118 9 0.0 6 9 119 12 0.0 6 12 120 12 0.0 6 12 121 11 0.0 6 11 122 16 0.0 6 16 123 13 0.0 6 13 124 21 0.0 6 21 125 12 0.0 6 12 126 15 0.0 6 15 127 14 0.0 6 14 128 18 0.0 6 18 129 20 0.0 6 20 130 20 0.0 6 20 131 22 0.0 6 22 132 22 0.0 6 22 133 13 0.0 6 13 134 17 0.0 6 16 1 135 40 0.0 6 40 136 25 0.0 6 25 137 38 0.0 6 38 138 28 0.0 6 28 139 30 0.0 6 30 140 41 0.0 6 41 141 71 0.0 6 71 142 488 0.0 6 488 143 234 0.0 6 234 144 132 0.0 6 132 145 324 0.0 6 324 146 186 0.0 6 186 147 254 0.0 6 254 148 66 0.0 6 66 149 74 0.0 6 74 150 187 0.0 6 187 151 127 0.0 6 127 152 665 0.0 6 664 0 0 1 153 235 0.0 6 235 154 129 0.0 6 129 155 74 0.0 6 73 0 0 0 0 0 1 156 332 0.0 6 332 157 270 0.0 6 270 158 118 0.0 6 118 159 473 0.0 6 473 160 309 0.0 6 309 161 505 0.0 6 505 162 121 0.0 6 120 1 163 308 0.0 6 308 164 323 0.0 6 323 165 355 0.0 6 355 166 117 0.0 6 117 167 141 0.0 6 141 168 633 0.0 6 632 1 169 368 0.0 6 365 2 0 0 1 170 380 0.0 6 380 171 775 0.0 6 773 1 0 0 0 1 172 194 0.0 6 193 1 173 448 0.0 6 448 174 113 0.0 6 112 1 175 148 0.0 6 147 0 0 0 0 1 176 884 0.0 6 884 177 440 0.0 6 440 178 86 0.0 6 86 179 121 0.0 6 121 180 280 0.0 6 279 1 181 133 0.0 6 131 2 182 226 0.0 6 226 183 152 0.0 6 150 2 184 691 0.0 6 690 1 185 448 0.0 6 447 1 186 179 0.0 6 179 187 726 0.0 6 724 1 1 188 286 0.0 6 286 189 650 0.0 6 649 0 0 0 0 1 190 279 0.0 6 279 191 80 0.0 6 80 192 84 0.0 6 83 0 0 0 0 0 1 193 129 0.0 6 129 194 70 0.0 6 70 195 155 0.0 6 154 0 0 0 1 196 206 0.0 6 206 197 202 0.0 6 202 198 615 0.0 6 614 1 199 296 0.0 6 295 1 200 421 0.0 6 420 1 201 175 0.0 6 174 1 202 257 0.0 6 256 1 203 515 0.0 6 515 204 201 0.0 6 201 205 169 0.0 6 169 206 220 0.0 6 219 1 207 356 0.0 6 355 1 208 450 0.0 6 448 2 209 680 0.0 6 680 210 397 0.0 6 396 1 211 336 0.0 6 336 212 845 0.0 6 844 0 1 213 403 0.0 6 403 214 582 0.0 6 582 215 367 0.0 6 365 1 0 0 0 0 1 216 768 0.0 6 767 1 217 333 0.0 6 333 218 571 0.0 6 570 1 219 413 0.0 6 413 220 473 0.0 6 473 221 442 0.0 6 442 222 793 0.0 6 793 223 533 0.0 6 532 1 224 204 0.0 6 204 225 307 0.0 6 307 226 406 0.0 6 404 2 227 165 0.0 6 164 1 228 215 0.0 6 214 1 229 129 0.0 6 129 230 143 0.0 6 142 0 0 0 0 0 1 231 144 0.0 6 143 1 232 155 0.0 6 155 233 225 0.0 6 224 1 234 214 0.0 6 213 1 235 197 0.0 6 197 236 187 0.0 6 185 2 237 150 0.0 6 149 1 238 199 0.0 6 199 239 255 0.0 6 255 240 286 0.0 6 286 241 539 0.0 6 539 242 170 0.0 6 170 243 291 0.0 6 291 244 119 0.0 6 118 1 245 114 0.0 6 114 246 124 0.0 6 124 247 129 0.0 6 129 248 135 0.0 6 135 249 157 0.0 6 157 250 244 0.0 6 244 251 206 0.0 6 205 1 252 154 0.0 6 154 253 121 0.0 6 121 254 149 0.0 6 149 255 154 0.0 6 154 256 70 0.0 6 70 257 101 0.0 6 101 258 160 0.0 6 160 259 68 0.0 6 67 1 260 86 0.0 6 86 261 121 0.0 6 121 262 46 0.0 6 46 263 76 0.0 6 75 0 1 264 113 0.0 6 112 1 265 101 0.0 6 101 266 109 0.0 6 109 267 110 0.0 6 110 268 66 0.0 6 66 269 80 0.0 6 80 270 66 0.0 6 66 271 102 0.0 6 102 272 154 0.0 6 154 273 146 0.0 6 146 274 46 0.0 6 46 275 82 0.0 6 82 276 86 0.0 6 86 277 73 0.0 6 73 278 72 0.0 6 72 279 332 0.0 6 332 280 532 0.0 6 527 3 2 281 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1282 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 1 0.0 6 1 72 9 0.0 6 9 73 16 0.0 6 16 74 6 0.0 6 6 75 3 0.0 6 3 76 14 0.0 6 14 77 9 0.0 6 9 78 10 0.0 6 10 79 1 0.0 6 1 80 1 0.0 6 1 81 5 0.0 6 5 82 4 0.0 6 4 83 19 0.0 6 19 84 9 0.0 6 9 85 2 0.0 6 2 86 4 0.0 6 4 87 10 0.0 6 10 88 7 0.0 6 7 89 4 0.0 6 4 90 12 0.0 6 12 91 16 0.0 6 16 92 13 0.0 6 13 93 5 0.0 6 5 94 5 0.0 6 5 95 14 0.0 6 14 96 10 0.0 6 10 98 7 0.0 6 7 99 15 0.0 6 15 100 11 0.0 6 11 101 17 0.0 6 17 102 25 0.0 6 25 103 3 0.0 6 3 104 17 0.0 6 17 105 1 0.0 6 1 106 7 0.0 6 7 107 33 0.0 6 33 108 17 0.0 6 17 110 2 0.0 6 2 111 8 0.0 6 8 112 3 0.0 6 3 113 11 0.0 6 11 114 7 0.0 6 7 115 30 0.0 6 30 116 12 0.0 6 12 117 10 0.0 6 10 118 26 0.0 6 25 1 119 15 0.0 6 14 0 0 0 0 0 1 120 15 0.0 6 15 125 1 0.0 6 1 126 3 0.0 6 3 127 8 0.0 6 8 128 8 0.0 6 8 129 12 0.0 6 12 130 6 0.0 6 6 131 12 0.0 6 12 132 3 0.0 6 3 133 9 0.0 6 9 134 23 0.0 6 23 135 1 0.0 6 1 137 1 0.0 6 1 139 4 0.0 6 4 140 17 0.0 6 17 141 1 0.0 6 1 142 11 0.0 6 11 143 41 0.0 6 41 144 7 0.0 6 7 145 26 0.0 6 26 146 12 0.0 6 12 147 22 0.0 6 22 148 1 0.0 6 1 149 1 0.0 6 1 151 1 0.0 6 1 152 16 0.0 6 16 153 50 0.0 6 50 154 47 0.0 6 47 155 1 0.0 6 1 156 5 0.0 6 5 157 39 0.0 6 39 158 10 0.0 6 10 159 10 0.0 6 10 160 2 0.0 6 2 166 2 0.0 6 2 167 5 0.0 6 5 168 1 0.0 6 1 169 2 0.0 6 2 170 4 0.0 6 4 171 11 0.0 6 11 172 33 0.0 6 33 173 9 0.0 6 9 174 16 0.0 6 16 175 2 0.0 6 2 179 3 0.0 6 3 181 9 0.0 6 9 182 26 0.0 6 26 183 3 0.0 6 3 184 7 0.0 6 7 185 17 0.0 6 17 186 32 0.0 6 32 187 1 0.0 6 1 188 3 0.0 6 3 202 4 0.0 6 4 203 5 0.0 6 5 204 8 0.0 6 8 205 17 0.0 6 17 213 7 0.0 6 7 214 16 0.0 6 16 215 2 0.0 6 2 221 11 0.0 6 11 222 11 0.0 6 11 231 2 0.0 6 2 235 3 0.0 6 3 236 26 0.0 6 26 237 22 0.0 6 22 238 3 0.0 6 3 250 1 0.0 6 1 251 5 0.0 6 5 252 8 0.0 6 8 261 8 0.0 6 8 262 11 0.0 6 11 263 2 0.0 6 2 270 1 0.0 6 1 281 1 0.0 6 1 282 3 0.0 6 3 283 4 0.0 6 4 291 1 0.0 6 1 297 1 0.0 6 1 306 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 38112 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 2.4 2 0 1 9 1 0.2 2 1 12 2 0.0 3 2 13 1 0.0 3 1 14 2 0.0 4 2 15 1 0.0 4 1 17 3 0.0 5 3 18 2 0.0 5 2 19 8 0.0 5 8 20 22 0.0 6 22 21 11 0.0 6 11 22 5 0.0 6 5 23 10 0.0 6 10 24 5 0.0 6 4 0 0 1 25 7 0.0 6 7 26 7 0.0 6 6 0 1 27 4 0.0 6 4 28 4 0.0 6 4 29 4 0.0 6 4 31 7 0.0 6 5 1 1 33 4 0.0 6 4 34 1 0.0 6 1 35 4 0.0 6 4 36 2 0.0 6 1 1 37 2 0.0 6 2 38 1 0.0 6 1 39 1 0.0 6 1 41 7 0.0 6 7 42 3 0.0 6 3 43 1 0.0 6 1 45 5 0.0 6 5 46 2 0.0 6 2 47 4 0.0 6 4 48 1 0.0 6 1 49 2 0.0 6 2 50 5 0.0 6 5 51 3 0.0 6 3 52 4 0.0 6 2 0 0 1 0 0 1 53 2 0.0 6 2 54 5 0.0 6 4 0 0 0 0 0 1 55 7 0.0 6 6 1 56 5 0.0 6 5 57 2 0.0 6 2 58 4 0.0 6 4 59 11 0.0 6 11 60 2 0.0 6 2 61 9 0.0 6 8 0 1 62 10 0.0 6 10 63 11 0.0 6 11 64 9 0.0 6 7 2 65 8 0.0 6 8 66 11 0.0 6 11 67 8 0.0 6 8 68 4 0.0 6 4 69 23 0.0 6 23 70 63 0.0 6 59 4 71 14 0.0 6 12 2 72 28 0.0 6 26 2 73 9 0.0 6 9 74 19 0.0 6 19 75 12 0.0 6 11 1 76 11 0.0 6 11 77 9 0.0 6 9 78 9 0.0 6 9 79 13 0.0 6 13 80 12 0.0 6 11 0 1 81 14 0.0 6 14 82 17 0.0 6 16 1 83 12 0.0 6 11 0 0 0 0 1 84 14 0.0 6 13 1 85 18 0.0 6 17 1 86 28 0.0 6 27 1 87 12 0.0 6 12 88 13 0.0 6 13 89 19 0.0 6 19 90 12 0.0 6 12 91 15 0.0 6 15 92 15 0.0 6 15 93 13 0.0 6 12 1 94 16 0.0 6 15 1 95 19 0.0 6 19 96 23 0.0 6 23 97 14 0.0 6 13 1 98 12 0.0 6 12 99 23 0.0 6 23 100 17 0.0 6 17 101 23 0.0 6 22 1 102 16 0.0 6 15 1 103 20 0.0 6 20 104 22 0.0 6 22 105 15 0.0 6 15 106 24 0.0 6 22 1 0 0 0 1 107 39 0.0 6 37 1 0 1 108 25 0.0 6 22 1 0 1 1 109 33 0.0 6 31 1 0 1 110 16 0.0 6 16 111 28 0.0 6 27 1 112 44 0.0 6 44 113 33 0.0 6 31 2 114 24 0.0 6 24 115 36 0.0 6 36 116 28 0.0 6 28 117 23 0.0 6 22 0 1 118 47 0.0 6 45 2 119 36 0.0 6 36 120 53 0.0 6 49 3 0 0 1 121 40 0.0 6 40 122 39 0.0 6 39 123 43 0.0 6 42 0 0 0 1 124 43 0.0 6 43 125 52 0.0 6 51 0 1 126 49 0.0 6 49 127 60 0.0 6 59 1 128 86 0.0 6 86 129 78 0.0 6 78 130 83 0.0 6 82 1 131 80 0.0 6 80 132 103 0.0 6 102 0 0 0 1 133 102 0.0 6 102 134 105 0.0 6 105 135 112 0.0 6 111 1 136 133 0.0 6 131 2 137 179 0.0 6 177 2 138 157 0.0 6 157 139 167 0.0 6 164 2 1 140 199 0.0 6 199 141 530 0.0 6 528 1 1 142 280 0.0 6 279 0 0 1 143 307 0.0 6 306 1 144 394 0.0 6 393 1 145 307 0.0 6 307 146 389 0.0 6 388 1 147 259 0.0 6 258 1 148 294 0.0 6 293 1 149 350 0.0 6 349 1 150 364 0.0 6 362 1 1 151 789 0.0 6 786 3 152 444 0.0 6 442 1 1 153 545 0.0 6 541 3 0 0 0 1 154 408 0.0 6 407 1 155 637 0.0 6 631 4 1 0 1 156 592 0.0 6 591 1 157 474 0.0 6 473 0 0 1 158 670 0.0 6 668 2 159 551 0.0 6 547 4 160 601 0.0 6 597 4 161 386 0.0 6 383 2 1 162 543 0.0 6 542 0 0 0 0 1 163 519 0.0 6 518 1 164 541 0.0 6 537 4 165 423 0.0 6 420 2 0 0 1 166 487 0.0 6 486 0 0 0 0 0 1 167 638 0.0 6 633 4 0 1 168 524 0.0 6 521 1 0 0 1 1 169 629 0.0 6 627 2 170 578 0.0 6 578 171 607 0.0 6 604 3 172 468 0.0 6 465 3 173 433 0.0 6 433 174 513 0.0 6 510 3 175 631 0.0 6 630 1 176 535 0.0 6 532 3 177 417 0.0 6 414 2 0 0 0 0 1 178 450 0.0 6 448 2 179 592 0.0 6 588 4 180 448 0.0 6 447 0 0 0 0 1 181 587 0.0 6 581 5 1 182 451 0.0 6 447 4 183 412 0.0 6 411 1 184 428 0.0 6 427 1 185 353 0.0 6 352 1 186 657 0.0 6 655 2 187 654 0.0 6 648 5 1 188 668 0.0 6 666 2 189 411 0.0 6 406 5 190 390 0.0 6 388 1 0 0 1 191 527 0.0 6 526 1 192 410 0.0 6 407 3 193 429 0.0 6 424 5 194 492 0.0 6 492 195 397 0.0 6 396 1 196 448 0.0 6 448 197 290 0.0 6 290 198 362 0.0 6 361 1 199 474 0.0 6 472 2 200 399 0.0 6 398 1 201 332 0.0 6 326 5 1 202 346 0.0 6 343 3 203 316 0.0 6 314 2 204 248 0.0 6 246 2 205 308 0.0 6 307 1 206 289 0.0 6 288 0 0 0 1 207 256 0.0 6 255 1 208 286 0.0 6 285 1 209 215 0.0 6 214 1 210 253 0.0 6 250 2 0 0 0 0 1 211 226 0.0 6 226 212 170 0.0 6 170 213 156 0.0 6 156 214 185 0.0 6 185 215 180 0.0 6 179 1 216 163 0.0 6 160 2 0 0 0 0 1 217 168 0.0 6 168 218 119 0.0 6 119 219 165 0.0 6 164 1 220 217 0.0 6 217 221 164 0.0 6 164 222 113 0.0 6 112 1 223 105 0.0 6 105 224 148 0.0 6 147 1 225 143 0.0 6 143 226 126 0.0 6 124 2 227 48 0.0 6 48 228 60 0.0 6 60 229 67 0.0 6 66 1 230 51 0.0 6 51 231 65 0.0 6 65 232 44 0.0 6 44 233 24 0.0 6 24 234 23 0.0 6 23 235 22 0.0 6 22 236 14 0.0 6 13 1 237 19 0.0 6 19 238 17 0.0 6 17 239 11 0.0 6 11 240 13 0.0 6 13 241 11 0.0 6 11 242 10 0.0 6 10 243 6 0.0 6 6 244 14 0.0 6 14 245 12 0.0 6 12 246 3 0.0 6 3 247 7 0.0 6 7 248 8 0.0 6 8 249 7 0.0 6 7 250 3 0.0 6 3 251 6 0.0 6 6 252 1 0.0 6 1 253 3 0.0 6 3 254 8 0.0 6 8 255 1 0.0 6 1 256 1 0.0 6 1 278 2 0.0 6 2 281 7 0.0 6 7 282 31 0.0 6 31 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1317 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 6 0.0 6 6 71 25 0.0 6 25 72 8 0.0 6 8 73 6 0.0 6 6 74 8 0.0 6 7 1 75 11 0.0 6 11 76 6 0.0 6 6 77 2 0.0 6 2 78 2 0.0 6 2 79 9 0.0 6 9 80 7 0.0 6 7 81 31 0.0 6 31 82 9 0.0 6 9 83 4 0.0 6 4 84 3 0.0 6 3 85 10 0.0 6 10 86 7 0.0 6 7 87 2 0.0 6 2 88 16 0.0 6 16 89 16 0.0 6 16 90 22 0.0 6 22 91 2 0.0 6 2 92 8 0.0 6 8 93 9 0.0 6 9 94 15 0.0 6 15 95 2 0.0 6 2 96 9 0.0 6 9 97 22 0.0 6 22 98 17 0.0 6 16 1 99 14 0.0 6 14 100 38 0.0 6 38 101 4 0.0 6 4 102 18 0.0 6 18 104 12 0.0 6 12 105 42 0.0 6 42 106 17 0.0 6 17 107 2 0.0 6 2 108 1 0.0 6 1 109 10 0.0 6 9 1 111 12 0.0 6 12 112 7 0.0 6 7 113 22 0.0 6 22 114 13 0.0 6 11 0 0 0 2 115 6 0.0 6 6 116 27 0.0 6 27 117 4 0.0 6 4 118 37 0.0 6 37 119 1 0.0 6 1 120 1 0.0 6 1 125 6 0.0 6 6 126 9 0.0 6 9 127 20 0.0 6 20 128 6 0.0 6 6 129 17 0.0 6 17 130 1 0.0 6 1 131 3 0.0 6 3 132 25 0.0 6 25 133 1 0.0 6 1 134 1 0.0 6 1 137 6 0.0 6 6 138 13 0.0 6 13 139 2 0.0 6 2 140 7 0.0 6 7 141 42 0.0 6 42 142 10 0.0 6 10 143 22 0.0 6 22 144 4 0.0 6 4 145 24 0.0 6 24 146 1 0.0 6 1 147 1 0.0 6 1 149 1 0.0 6 1 150 11 0.0 6 11 151 61 0.0 6 61 152 59 0.0 6 59 153 4 0.0 6 4 154 21 0.0 6 21 155 50 0.0 6 50 156 5 0.0 6 5 157 12 0.0 6 12 158 2 0.0 6 1 0 0 1 164 1 0.0 6 1 165 4 0.0 6 4 166 1 0.0 6 1 167 1 0.0 6 1 168 6 0.0 6 6 169 15 0.0 6 15 170 35 0.0 6 35 171 6 0.0 6 6 172 28 0.0 6 28 178 2 0.0 6 2 179 3 0.0 6 3 180 27 0.0 6 27 182 6 0.0 6 6 183 18 0.0 6 18 184 43 0.0 6 43 185 2 0.0 6 2 186 2 0.0 6 2 188 1 0.0 6 0 0 0 0 1 201 3 0.0 6 3 202 5 0.0 6 5 203 12 0.0 6 12 212 17 0.0 6 17 219 2 0.0 6 2 220 12 0.0 6 12 221 1 0.0 6 1 233 4 0.0 6 4 234 7 0.0 6 7 235 17 0.0 6 17 248 1 0.0 6 1 249 1 0.0 6 1 250 2 0.0 6 2 259 1 0.0 6 1 260 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_12_162_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_12_389_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_12_162_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_12_389_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.77 s (48 µs/read; 1.26 M reads/minute). === Summary === Total read pairs processed: 37,024 Read 1 with adapter: 37,021 (100.0%) Read 2 with adapter: 37,000 (99.9%) == Read fate breakdown == Pairs that were too short: 10 (0.0%) Pairs discarded as untrimmed: 27 (0.1%) Pairs written (passing filters): 36,987 (99.9%) Total basepairs processed: 15,826,718 bp Read 1: 8,857,782 bp Read 2: 6,968,936 bp Total written (filtered): 2,356,039 bp (14.9%) Read 1: 1,190,014 bp Read 2: 1,166,025 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 36154 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 578.5 0 1 4 2 144.6 1 0 2 6 1 9.0 1 1 7 1 2.3 2 1 9 1 0.1 2 1 13 1 0.0 3 1 17 2 0.0 5 2 19 1 0.0 5 1 20 35 0.0 6 35 21 18 0.0 6 17 1 22 20 0.0 6 20 23 16 0.0 6 15 1 24 12 0.0 6 11 1 25 8 0.0 6 7 1 26 6 0.0 6 6 27 7 0.0 6 5 2 28 8 0.0 6 7 1 29 4 0.0 6 4 30 2 0.0 6 1 1 31 4 0.0 6 4 32 2 0.0 6 2 33 3 0.0 6 3 34 2 0.0 6 2 35 7 0.0 6 7 36 1 0.0 6 1 37 3 0.0 6 3 39 4 0.0 6 3 1 40 6 0.0 6 6 41 3 0.0 6 3 42 2 0.0 6 1 1 43 3 0.0 6 3 44 4 0.0 6 4 45 2 0.0 6 2 47 5 0.0 6 5 48 1 0.0 6 1 49 2 0.0 6 1 1 50 2 0.0 6 2 51 6 0.0 6 6 52 1 0.0 6 1 54 1 0.0 6 1 55 1 0.0 6 1 56 2 0.0 6 2 57 2 0.0 6 2 58 2 0.0 6 2 59 3 0.0 6 3 60 3 0.0 6 3 61 4 0.0 6 4 62 3 0.0 6 3 63 3 0.0 6 3 64 3 0.0 6 3 65 1 0.0 6 1 66 4 0.0 6 4 67 1 0.0 6 1 68 1 0.0 6 1 69 4 0.0 6 4 70 2 0.0 6 2 71 5 0.0 6 4 1 72 4 0.0 6 4 73 4 0.0 6 4 74 5 0.0 6 5 75 4 0.0 6 4 76 6 0.0 6 6 77 3 0.0 6 3 78 2 0.0 6 2 79 3 0.0 6 3 80 3 0.0 6 3 81 4 0.0 6 4 82 6 0.0 6 6 83 5 0.0 6 5 84 5 0.0 6 5 85 5 0.0 6 5 86 5 0.0 6 5 87 3 0.0 6 3 88 5 0.0 6 5 89 6 0.0 6 6 90 6 0.0 6 5 1 91 8 0.0 6 7 1 92 2 0.0 6 2 93 3 0.0 6 3 94 5 0.0 6 4 1 95 4 0.0 6 4 96 1 0.0 6 0 1 97 10 0.0 6 10 98 8 0.0 6 8 99 6 0.0 6 6 100 6 0.0 6 6 101 5 0.0 6 5 102 8 0.0 6 8 103 7 0.0 6 7 104 13 0.0 6 12 1 105 9 0.0 6 9 106 11 0.0 6 11 107 5 0.0 6 5 108 8 0.0 6 8 109 6 0.0 6 6 110 9 0.0 6 9 111 11 0.0 6 11 112 6 0.0 6 6 113 12 0.0 6 12 114 11 0.0 6 11 115 11 0.0 6 11 116 14 0.0 6 13 1 117 19 0.0 6 19 118 16 0.0 6 16 119 12 0.0 6 12 120 10 0.0 6 9 1 121 8 0.0 6 8 122 8 0.0 6 8 123 15 0.0 6 15 124 18 0.0 6 17 1 125 31 0.0 6 30 1 126 17 0.0 6 14 3 127 20 0.0 6 19 1 128 9 0.0 6 9 129 22 0.0 6 20 2 130 21 0.0 6 21 131 23 0.0 6 22 1 132 14 0.0 6 13 1 133 19 0.0 6 19 134 17 0.0 6 17 135 24 0.0 6 23 1 136 23 0.0 6 23 137 18 0.0 6 17 0 0 0 0 1 138 20 0.0 6 20 139 30 0.0 6 30 140 31 0.0 6 31 141 45 0.0 6 45 142 133 0.0 6 131 1 1 143 224 0.0 6 224 144 375 0.0 6 373 2 145 187 0.0 6 187 146 181 0.0 6 180 1 147 262 0.0 6 262 148 230 0.0 6 230 149 218 0.0 6 218 150 237 0.0 6 237 151 206 0.0 6 205 1 152 266 0.0 6 265 1 153 217 0.0 6 217 154 165 0.0 6 163 2 155 160 0.0 6 159 1 156 161 0.0 6 161 157 189 0.0 6 188 1 158 157 0.0 6 157 159 210 0.0 6 209 1 160 173 0.0 6 173 161 171 0.0 6 171 162 209 0.0 6 209 163 164 0.0 6 164 164 152 0.0 6 152 165 180 0.0 6 180 166 150 0.0 6 150 167 143 0.0 6 143 168 189 0.0 6 188 1 169 195 0.0 6 194 1 170 150 0.0 6 150 171 155 0.0 6 154 1 172 161 0.0 6 159 2 173 150 0.0 6 149 1 174 202 0.0 6 201 1 175 233 0.0 6 232 1 176 149 0.0 6 149 177 176 0.0 6 173 2 0 0 0 1 178 191 0.0 6 190 1 179 216 0.0 6 215 1 180 577 0.0 6 576 1 181 208 0.0 6 208 182 149 0.0 6 148 1 183 209 0.0 6 207 2 184 179 0.0 6 179 185 183 0.0 6 180 3 186 200 0.0 6 199 1 187 220 0.0 6 220 188 185 0.0 6 185 189 264 0.0 6 262 1 1 190 199 0.0 6 197 2 191 203 0.0 6 203 192 268 0.0 6 268 193 267 0.0 6 266 1 194 253 0.0 6 250 3 195 297 0.0 6 293 3 1 196 340 0.0 6 336 3 0 0 1 197 364 0.0 6 364 198 463 0.0 6 460 2 0 0 0 1 199 406 0.0 6 405 1 200 351 0.0 6 350 1 201 341 0.0 6 340 1 202 365 0.0 6 365 203 319 0.0 6 317 2 204 471 0.0 6 468 3 205 372 0.0 6 370 2 206 349 0.0 6 346 3 207 488 0.0 6 486 1 1 208 364 0.0 6 360 3 0 1 209 395 0.0 6 391 4 210 324 0.0 6 323 1 211 339 0.0 6 336 3 212 368 0.0 6 365 3 213 441 0.0 6 437 4 214 400 0.0 6 398 2 215 394 0.0 6 392 2 216 560 0.0 6 555 5 217 404 0.0 6 399 4 0 0 1 218 437 0.0 6 435 2 219 426 0.0 6 424 2 220 385 0.0 6 382 3 221 332 0.0 6 329 3 222 396 0.0 6 394 1 0 0 0 0 1 223 300 0.0 6 299 1 224 333 0.0 6 329 4 225 376 0.0 6 374 1 0 1 226 407 0.0 6 407 227 425 0.0 6 425 228 369 0.0 6 366 3 229 301 0.0 6 299 2 230 308 0.0 6 307 1 231 322 0.0 6 321 1 232 316 0.0 6 315 0 0 0 0 1 233 329 0.0 6 327 2 234 371 0.0 6 369 1 0 0 1 235 259 0.0 6 257 1 0 0 0 1 236 266 0.0 6 264 2 237 301 0.0 6 299 2 238 275 0.0 6 274 0 1 239 257 0.0 6 257 240 275 0.0 6 272 2 1 241 234 0.0 6 234 242 249 0.0 6 247 2 243 260 0.0 6 259 0 0 0 1 244 214 0.0 6 213 1 245 172 0.0 6 170 1 0 0 0 1 246 181 0.0 6 181 247 153 0.0 6 153 248 169 0.0 6 168 1 249 211 0.0 6 210 1 250 173 0.0 6 173 251 158 0.0 6 155 3 252 159 0.0 6 159 253 160 0.0 6 160 254 137 0.0 6 137 255 135 0.0 6 134 1 256 141 0.0 6 141 257 125 0.0 6 124 1 258 142 0.0 6 140 2 259 159 0.0 6 158 1 260 151 0.0 6 151 261 136 0.0 6 133 2 1 262 100 0.0 6 99 0 0 0 1 263 101 0.0 6 101 264 91 0.0 6 90 1 265 97 0.0 6 97 266 98 0.0 6 97 1 267 95 0.0 6 93 2 268 89 0.0 6 87 2 269 111 0.0 6 111 270 92 0.0 6 92 271 76 0.0 6 75 1 272 100 0.0 6 100 273 97 0.0 6 97 274 98 0.0 6 98 275 102 0.0 6 102 276 133 0.0 6 131 1 1 277 183 0.0 6 180 3 278 295 0.0 6 293 2 279 1126 0.0 6 1106 19 1 280 887 0.0 6 879 6 2 281 4 0.0 6 3 1 282 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 867 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 5 0.0 6 1 3 1 62 92 0.0 6 90 2 70 1 0.0 6 1 71 5 0.0 6 5 72 8 0.0 6 8 73 9 0.0 6 9 74 3 0.0 6 3 75 6 0.0 6 6 76 4 0.0 6 4 77 9 0.0 6 9 78 11 0.0 6 11 79 6 0.0 6 6 80 7 0.0 6 7 81 6 0.0 6 6 82 4 0.0 6 4 83 1 0.0 6 1 84 8 0.0 6 8 86 3 0.0 6 3 87 6 0.0 6 5 0 0 0 0 0 1 88 4 0.0 6 4 89 4 0.0 6 4 90 5 0.0 6 5 91 2 0.0 6 2 92 4 0.0 6 4 93 3 0.0 6 3 94 8 0.0 6 8 95 7 0.0 6 7 96 4 0.0 6 4 97 4 0.0 6 4 98 3 0.0 6 3 99 3 0.0 6 3 100 2 0.0 6 2 101 3 0.0 6 3 102 3 0.0 6 3 103 4 0.0 6 4 104 2 0.0 6 2 105 1 0.0 6 1 106 5 0.0 6 5 107 3 0.0 6 3 108 7 0.0 6 7 109 10 0.0 6 10 110 4 0.0 6 4 111 3 0.0 6 3 112 7 0.0 6 7 113 1 0.0 6 1 114 6 0.0 6 6 115 3 0.0 6 3 116 4 0.0 6 4 117 5 0.0 6 5 118 3 0.0 6 3 120 5 0.0 6 5 121 4 0.0 6 4 122 3 0.0 6 3 123 2 0.0 6 2 124 1 0.0 6 1 125 3 0.0 6 3 126 5 0.0 6 5 127 8 0.0 6 8 128 5 0.0 6 5 129 1 0.0 6 1 130 2 0.0 6 2 131 3 0.0 6 3 132 7 0.0 6 7 133 7 0.0 6 7 134 5 0.0 6 5 135 2 0.0 6 2 136 6 0.0 6 6 137 3 0.0 6 3 138 8 0.0 6 8 139 6 0.0 6 6 140 2 0.0 6 2 141 8 0.0 6 8 142 8 0.0 6 8 143 2 0.0 6 2 144 3 0.0 6 3 145 2 0.0 6 2 146 5 0.0 6 5 147 6 0.0 6 6 148 8 0.0 6 8 149 3 0.0 6 3 150 4 0.0 6 4 151 2 0.0 6 2 152 2 0.0 6 2 153 5 0.0 6 5 154 4 0.0 6 4 156 6 0.0 6 6 157 6 0.0 6 6 158 2 0.0 6 2 159 4 0.0 6 4 160 6 0.0 6 6 161 9 0.0 6 9 162 6 0.0 6 6 163 11 0.0 6 11 164 8 0.0 6 8 165 6 0.0 6 6 166 5 0.0 6 5 167 5 0.0 6 5 168 7 0.0 6 7 169 6 0.0 6 6 170 3 0.0 6 3 171 5 0.0 6 5 172 3 0.0 6 3 173 5 0.0 6 5 174 2 0.0 6 2 175 7 0.0 6 7 176 6 0.0 6 6 177 6 0.0 6 6 178 2 0.0 6 2 179 1 0.0 6 1 180 2 0.0 6 2 181 1 0.0 6 1 182 3 0.0 6 3 183 3 0.0 6 3 184 8 0.0 6 8 185 3 0.0 6 3 186 4 0.0 6 4 187 4 0.0 6 4 188 6 0.0 6 6 189 7 0.0 6 7 190 6 0.0 6 6 191 1 0.0 6 1 192 4 0.0 6 4 193 4 0.0 6 4 194 3 0.0 6 3 195 5 0.0 6 5 196 2 0.0 6 2 197 5 0.0 6 5 198 3 0.0 6 3 199 4 0.0 6 4 200 3 0.0 6 3 201 7 0.0 6 7 202 2 0.0 6 2 203 4 0.0 6 3 1 204 5 0.0 6 5 205 7 0.0 6 7 206 7 0.0 6 7 207 3 0.0 6 3 208 3 0.0 6 3 209 3 0.0 6 3 210 6 0.0 6 6 211 4 0.0 6 4 212 1 0.0 6 1 213 2 0.0 6 2 214 3 0.0 6 3 216 4 0.0 6 4 217 9 0.0 6 9 218 3 0.0 6 3 219 2 0.0 6 2 220 6 0.0 6 6 221 4 0.0 6 4 222 2 0.0 6 2 223 2 0.0 6 2 224 1 0.0 6 1 225 1 0.0 6 1 226 6 0.0 6 6 227 5 0.0 6 5 228 5 0.0 6 5 229 5 0.0 6 5 230 4 0.0 6 4 231 3 0.0 6 3 232 2 0.0 6 2 233 3 0.0 6 3 234 6 0.0 6 6 236 1 0.0 6 1 237 1 0.0 6 1 238 1 0.0 6 1 239 3 0.0 6 3 240 1 0.0 6 1 241 4 0.0 6 4 242 1 0.0 6 1 243 2 0.0 6 2 244 4 0.0 6 4 245 2 0.0 6 2 246 3 0.0 6 3 247 2 0.0 6 2 248 1 0.0 6 1 250 2 0.0 6 2 251 1 0.0 6 1 252 5 0.0 6 5 253 1 0.0 6 1 256 1 0.0 6 1 258 4 0.0 6 3 1 260 2 0.0 6 2 261 1 0.0 6 1 263 1 0.0 6 1 264 1 0.0 6 1 267 1 0.0 6 1 268 1 0.0 6 1 270 1 0.0 6 1 272 1 0.0 6 1 275 2 0.0 6 2 276 1 0.0 6 1 277 1 0.0 6 1 278 1 0.0 6 1 285 1 0.0 6 1 286 1 0.0 6 1 293 1 0.0 6 1 294 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 36350 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 578.5 0 0 3 11 2 0.0 3 2 12 4 0.0 3 4 13 6 0.0 3 5 1 14 5 0.0 4 5 15 1 0.0 4 1 16 1 0.0 4 1 17 17 0.0 5 17 18 12 0.0 5 9 2 1 19 12 0.0 5 11 1 20 24 0.0 6 23 1 21 16 0.0 6 12 3 0 1 22 12 0.0 6 10 2 23 8 0.0 6 8 24 8 0.0 6 8 25 6 0.0 6 6 26 11 0.0 6 10 1 27 7 0.0 6 5 2 28 6 0.0 6 6 29 4 0.0 6 4 30 7 0.0 6 6 1 31 4 0.0 6 4 32 6 0.0 6 6 33 5 0.0 6 5 34 3 0.0 6 2 1 35 7 0.0 6 7 36 5 0.0 6 5 37 3 0.0 6 3 38 11 0.0 6 9 1 1 39 11 0.0 6 11 40 7 0.0 6 7 41 9 0.0 6 7 1 0 1 42 8 0.0 6 7 0 0 0 0 1 43 10 0.0 6 9 1 44 6 0.0 6 5 1 45 2 0.0 6 2 46 7 0.0 6 7 47 10 0.0 6 9 1 48 9 0.0 6 9 49 9 0.0 6 8 0 1 50 4 0.0 6 3 1 51 10 0.0 6 8 2 52 22 0.0 6 21 1 53 10 0.0 6 10 54 9 0.0 6 7 2 55 13 0.0 6 12 1 56 11 0.0 6 8 3 57 10 0.0 6 9 0 1 58 19 0.0 6 19 59 14 0.0 6 13 1 60 10 0.0 6 8 1 1 61 14 0.0 6 12 2 62 10 0.0 6 9 1 63 10 0.0 6 9 1 64 12 0.0 6 11 1 65 25 0.0 6 25 66 14 0.0 6 14 67 26 0.0 6 26 68 69 0.0 6 65 4 69 30 0.0 6 29 1 70 32 0.0 6 29 3 71 16 0.0 6 15 0 1 72 19 0.0 6 19 73 25 0.0 6 21 4 74 14 0.0 6 13 1 75 18 0.0 6 17 1 76 23 0.0 6 21 2 77 9 0.0 6 9 78 17 0.0 6 16 1 79 18 0.0 6 15 2 0 0 1 80 28 0.0 6 26 2 81 33 0.0 6 33 82 28 0.0 6 26 2 83 28 0.0 6 25 3 84 42 0.0 6 39 3 85 21 0.0 6 21 86 24 0.0 6 23 0 1 87 25 0.0 6 24 1 88 23 0.0 6 21 2 89 29 0.0 6 28 1 90 26 0.0 6 25 1 91 51 0.0 6 50 1 92 40 0.0 6 38 2 93 41 0.0 6 37 3 0 1 94 49 0.0 6 48 1 95 43 0.0 6 43 96 46 0.0 6 44 1 0 1 97 37 0.0 6 36 1 98 42 0.0 6 40 2 99 45 0.0 6 44 1 100 35 0.0 6 35 101 45 0.0 6 43 2 102 58 0.0 6 52 5 1 103 45 0.0 6 44 0 0 0 1 104 68 0.0 6 67 1 105 86 0.0 6 81 4 0 1 106 61 0.0 6 57 4 107 58 0.0 6 58 108 59 0.0 6 57 1 0 0 0 1 109 57 0.0 6 55 1 0 1 110 73 0.0 6 69 2 1 0 0 1 111 56 0.0 6 53 3 112 81 0.0 6 79 2 113 103 0.0 6 101 2 114 76 0.0 6 73 3 115 85 0.0 6 83 1 0 0 1 116 97 0.0 6 96 1 117 75 0.0 6 73 0 1 0 1 118 96 0.0 6 95 1 119 99 0.0 6 98 0 1 120 101 0.0 6 95 5 0 0 0 0 1 121 80 0.0 6 80 122 90 0.0 6 86 3 0 0 1 123 97 0.0 6 97 124 127 0.0 6 126 1 125 128 0.0 6 127 0 0 1 126 153 0.0 6 151 1 1 127 172 0.0 6 170 1 0 0 1 128 139 0.0 6 137 2 129 172 0.0 6 170 1 1 130 230 0.0 6 222 7 0 1 131 236 0.0 6 233 3 132 225 0.0 6 223 2 133 244 0.0 6 236 5 0 1 1 1 134 246 0.0 6 236 7 1 0 1 0 1 135 292 0.0 6 288 4 136 307 0.0 6 301 5 0 0 1 137 267 0.0 6 266 0 0 0 0 0 1 138 261 0.0 6 259 2 139 383 0.0 6 375 7 0 0 1 140 456 0.0 6 450 6 141 533 0.0 6 531 2 142 472 0.0 6 463 9 143 475 0.0 6 471 3 0 0 1 144 541 0.0 6 532 8 0 0 0 1 145 543 0.0 6 538 3 0 1 0 0 1 146 552 0.0 6 546 6 147 623 0.0 6 616 7 148 546 0.0 6 539 6 0 0 0 0 1 149 661 0.0 6 643 17 0 1 150 763 0.0 6 751 10 0 1 1 151 841 0.0 6 826 13 1 1 152 689 0.0 6 678 8 1 0 0 2 153 797 0.0 6 785 8 3 1 154 738 0.0 6 730 6 1 0 0 0 1 155 654 0.0 6 648 4 0 0 1 1 156 652 0.0 6 644 7 0 0 0 0 1 157 647 0.0 6 643 4 158 618 0.0 6 607 10 0 0 0 0 1 159 647 0.0 6 638 6 1 1 0 1 160 621 0.0 6 618 3 161 534 0.0 6 529 4 0 0 0 0 1 162 516 0.0 6 510 4 0 0 1 1 163 438 0.0 6 435 3 164 513 0.0 6 509 3 1 165 435 0.0 6 433 2 166 430 0.0 6 421 7 1 0 1 167 536 0.0 6 528 7 0 0 0 1 168 471 0.0 6 467 4 169 552 0.0 6 547 5 170 459 0.0 6 454 3 1 0 1 171 445 0.0 6 442 3 172 547 0.0 6 540 6 0 0 0 1 173 498 0.0 6 489 8 0 0 0 1 174 512 0.0 6 506 5 1 175 535 0.0 6 532 3 176 475 0.0 6 470 5 177 537 0.0 6 531 3 0 1 0 0 2 178 373 0.0 6 368 3 1 0 1 179 351 0.0 6 348 3 180 269 0.0 6 269 181 313 0.0 6 311 2 182 254 0.0 6 254 183 235 0.0 6 232 2 0 1 184 385 0.0 6 379 4 0 2 185 478 0.0 6 477 1 186 417 0.0 6 412 5 187 317 0.0 6 311 6 188 269 0.0 6 266 3 189 246 0.0 6 245 1 190 300 0.0 6 299 1 191 261 0.0 6 255 5 0 0 1 192 223 0.0 6 221 2 193 178 0.0 6 177 1 194 172 0.0 6 169 2 0 0 1 195 184 0.0 6 183 1 196 205 0.0 6 204 1 197 199 0.0 6 198 1 198 241 0.0 6 239 2 199 210 0.0 6 207 2 0 0 1 200 139 0.0 6 136 3 201 131 0.0 6 130 1 202 122 0.0 6 121 0 0 0 1 203 114 0.0 6 113 1 204 94 0.0 6 92 2 205 78 0.0 6 78 206 76 0.0 6 76 207 67 0.0 6 64 2 0 0 0 0 1 208 64 0.0 6 64 209 66 0.0 6 65 1 210 48 0.0 6 48 211 40 0.0 6 40 212 37 0.0 6 37 213 29 0.0 6 29 214 54 0.0 6 53 1 215 67 0.0 6 64 3 216 50 0.0 6 49 1 217 41 0.0 6 39 2 218 31 0.0 6 31 219 48 0.0 6 46 2 220 29 0.0 6 28 1 221 33 0.0 6 33 222 23 0.0 6 23 223 48 0.0 6 46 2 224 40 0.0 6 40 225 30 0.0 6 30 226 13 0.0 6 13 227 11 0.0 6 10 1 228 27 0.0 6 26 1 229 17 0.0 6 17 230 20 0.0 6 19 1 231 12 0.0 6 12 232 22 0.0 6 20 2 233 17 0.0 6 16 1 234 11 0.0 6 11 235 4 0.0 6 4 236 3 0.0 6 3 238 1 0.0 6 1 239 6 0.0 6 5 1 240 7 0.0 6 7 241 12 0.0 6 11 1 242 8 0.0 6 8 243 7 0.0 6 7 244 3 0.0 6 3 245 4 0.0 6 4 246 5 0.0 6 5 247 3 0.0 6 3 248 1 0.0 6 1 249 2 0.0 6 2 250 1 0.0 6 1 252 1 0.0 6 1 253 1 0.0 6 1 254 1 0.0 6 1 257 1 0.0 6 1 279 3 0.0 6 3 280 43 0.0 6 43 281 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 650 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 54 20 0.0 6 20 59 2 0.0 6 2 71 8 0.0 6 8 72 8 0.0 6 8 73 12 0.0 6 12 74 5 0.0 6 5 75 7 0.0 6 7 76 5 0.0 6 5 77 4 0.0 6 4 78 6 0.0 6 6 79 6 0.0 6 6 80 13 0.0 6 13 81 5 0.0 6 5 82 7 0.0 6 7 83 8 0.0 6 8 84 4 0.0 6 4 85 4 0.0 6 4 86 4 0.0 6 4 87 1 0.0 6 1 88 9 0.0 6 9 89 4 0.0 6 4 90 4 0.0 6 4 91 3 0.0 6 3 92 5 0.0 6 5 93 3 0.0 6 3 94 3 0.0 6 3 95 2 0.0 6 1 1 97 8 0.0 6 8 98 3 0.0 6 3 100 3 0.0 6 3 101 4 0.0 6 4 102 4 0.0 6 4 103 6 0.0 6 6 104 1 0.0 6 1 105 7 0.0 6 7 106 2 0.0 6 2 107 9 0.0 6 9 108 6 0.0 6 6 109 15 0.0 6 15 110 4 0.0 6 4 111 2 0.0 6 2 112 3 0.0 6 3 113 4 0.0 6 4 114 2 0.0 6 2 115 4 0.0 6 4 116 2 0.0 6 1 0 0 0 1 117 3 0.0 6 2 1 118 9 0.0 6 9 119 3 0.0 6 3 120 5 0.0 6 5 121 2 0.0 6 2 122 2 0.0 6 2 123 4 0.0 6 4 124 6 0.0 6 6 125 4 0.0 6 4 126 3 0.0 6 3 127 8 0.0 6 8 128 1 0.0 6 1 129 1 0.0 6 1 130 9 0.0 6 9 131 4 0.0 6 3 1 132 5 0.0 6 5 133 19 0.0 6 19 134 4 0.0 6 4 135 3 0.0 6 3 136 3 0.0 6 3 137 6 0.0 6 6 138 7 0.0 6 7 139 1 0.0 6 1 140 7 0.0 6 7 141 10 0.0 6 10 142 4 0.0 6 4 143 10 0.0 6 10 144 5 0.0 6 5 145 4 0.0 6 4 147 8 0.0 6 8 148 7 0.0 6 7 149 6 0.0 6 5 1 150 2 0.0 6 2 151 7 0.0 6 7 152 4 0.0 6 4 153 5 0.0 6 5 154 7 0.0 6 7 155 4 0.0 6 4 156 4 0.0 6 4 157 8 0.0 6 8 158 1 0.0 6 1 159 2 0.0 6 2 160 11 0.0 6 11 161 6 0.0 6 6 162 6 0.0 6 6 163 6 0.0 6 6 164 2 0.0 6 2 165 5 0.0 6 5 166 8 0.0 6 8 167 8 0.0 6 7 1 168 7 0.0 6 7 169 2 0.0 6 2 170 1 0.0 6 1 171 4 0.0 6 4 172 8 0.0 6 8 173 6 0.0 6 6 174 2 0.0 6 2 175 3 0.0 6 3 176 3 0.0 6 3 177 5 0.0 6 5 178 5 0.0 6 5 179 2 0.0 6 2 180 3 0.0 6 3 181 4 0.0 6 4 184 3 0.0 6 3 185 2 0.0 6 2 186 3 0.0 6 3 187 4 0.0 6 4 188 8 0.0 6 8 189 3 0.0 6 3 190 5 0.0 6 5 191 4 0.0 6 4 192 1 0.0 6 1 193 2 0.0 6 2 195 1 0.0 6 1 196 4 0.0 6 4 197 2 0.0 6 2 198 3 0.0 6 3 199 4 0.0 6 4 201 1 0.0 6 1 203 3 0.0 6 3 206 2 0.0 6 2 207 2 0.0 6 2 210 2 0.0 6 2 212 2 0.0 6 2 213 1 0.0 6 1 214 1 0.0 6 1 216 1 0.0 6 1 217 2 0.0 6 2 219 1 0.0 6 1 220 1 0.0 6 1 221 2 0.0 6 2 227 1 0.0 6 1 228 1 0.0 6 1 229 1 0.0 6 1 237 1 0.0 6 1 242 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_13_163_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_13_390_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_13_163_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_13_390_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.91 s (40 µs/read; 1.48 M reads/minute). === Summary === Total read pairs processed: 47,225 Read 1 with adapter: 47,221 (100.0%) Read 2 with adapter: 47,195 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 34 (0.1%) Pairs written (passing filters): 47,191 (99.9%) Total basepairs processed: 24,298,067 bp Read 1: 14,029,206 bp Read 2: 10,268,861 bp Total written (filtered): 2,879,398 bp (11.9%) Read 1: 1,463,706 bp Read 2: 1,415,692 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 47206 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 2.9 2 1 9 1 0.2 2 1 17 1 0.0 5 1 20 6 0.0 6 6 21 3 0.0 6 3 22 1 0.0 6 1 23 1 0.0 6 1 25 7 0.0 6 7 26 4 0.0 6 3 1 27 1 0.0 6 1 28 2 0.0 6 2 29 2 0.0 6 2 30 2 0.0 6 2 34 3 0.0 6 1 2 35 1 0.0 6 1 36 1 0.0 6 1 38 2 0.0 6 1 1 39 1 0.0 6 1 40 2 0.0 6 2 42 1 0.0 6 1 43 1 0.0 6 1 44 8 0.0 6 8 45 2 0.0 6 2 46 1 0.0 6 1 48 1 0.0 6 1 50 1 0.0 6 1 53 2 0.0 6 2 54 2 0.0 6 2 56 3 0.0 6 3 57 1 0.0 6 1 60 1 0.0 6 1 62 3 0.0 6 2 1 63 2 0.0 6 2 64 2 0.0 6 2 65 1 0.0 6 1 66 1 0.0 6 1 68 2 0.0 6 2 70 1 0.0 6 1 71 1 0.0 6 1 73 1 0.0 6 1 74 1 0.0 6 1 76 1 0.0 6 1 77 1 0.0 6 1 78 2 0.0 6 2 79 1 0.0 6 1 81 1 0.0 6 1 83 1 0.0 6 1 86 11 0.0 6 11 87 2 0.0 6 2 89 2 0.0 6 2 90 3 0.0 6 3 91 2 0.0 6 1 1 92 1 0.0 6 1 96 1 0.0 6 1 97 4 0.0 6 4 98 4 0.0 6 4 100 5 0.0 6 5 101 8 0.0 6 8 102 1 0.0 6 1 103 3 0.0 6 3 104 2 0.0 6 1 1 105 3 0.0 6 3 106 1 0.0 6 1 107 9 0.0 6 9 108 5 0.0 6 5 109 5 0.0 6 5 112 4 0.0 6 4 113 4 0.0 6 4 114 4 0.0 6 4 115 10 0.0 6 9 1 116 1 0.0 6 1 117 3 0.0 6 3 118 7 0.0 6 6 1 119 2 0.0 6 2 121 4 0.0 6 3 1 122 8 0.0 6 7 1 123 7 0.0 6 7 124 5 0.0 6 5 125 2 0.0 6 2 126 6 0.0 6 6 127 2 0.0 6 2 128 6 0.0 6 5 0 0 0 0 1 129 7 0.0 6 7 130 6 0.0 6 6 131 6 0.0 6 6 132 15 0.0 6 15 133 4 0.0 6 4 134 9 0.0 6 9 135 12 0.0 6 12 136 11 0.0 6 11 137 4 0.0 6 4 138 5 0.0 6 5 139 4 0.0 6 4 140 10 0.0 6 10 141 67 0.0 6 66 1 142 39 0.0 6 39 143 11 0.0 6 10 1 144 8 0.0 6 6 1 0 0 1 145 22 0.0 6 20 2 146 13 0.0 6 12 1 147 34 0.0 6 34 148 55 0.0 6 53 2 149 19 0.0 6 18 1 150 16 0.0 6 15 1 151 20 0.0 6 17 3 152 14 0.0 6 13 1 153 13 0.0 6 12 1 154 13 0.0 6 13 155 4 0.0 6 4 156 6 0.0 6 6 157 6 0.0 6 6 158 29 0.0 6 28 1 159 15 0.0 6 15 160 16 0.0 6 16 161 14 0.0 6 14 162 16 0.0 6 16 163 17 0.0 6 16 1 164 8 0.0 6 7 1 165 18 0.0 6 18 166 12 0.0 6 11 1 167 10 0.0 6 10 168 26 0.0 6 24 2 169 17 0.0 6 17 170 11 0.0 6 11 171 12 0.0 6 12 172 12 0.0 6 12 173 22 0.0 6 21 1 174 26 0.0 6 24 2 175 31 0.0 6 28 3 176 29 0.0 6 29 177 36 0.0 6 36 178 15 0.0 6 14 1 179 22 0.0 6 22 180 18 0.0 6 17 1 181 19 0.0 6 18 1 182 26 0.0 6 23 3 183 42 0.0 6 40 1 1 184 39 0.0 6 38 1 185 65 0.0 6 64 1 186 49 0.0 6 48 1 187 43 0.0 6 43 188 61 0.0 6 60 1 189 68 0.0 6 65 3 190 61 0.0 6 59 2 191 86 0.0 6 82 3 1 192 79 0.0 6 78 1 193 106 0.0 6 104 2 194 63 0.0 6 63 195 119 0.0 6 115 4 196 126 0.0 6 121 5 197 86 0.0 6 81 5 198 124 0.0 6 121 2 1 199 103 0.0 6 94 9 200 79 0.0 6 76 3 201 108 0.0 6 103 5 202 135 0.0 6 133 2 203 96 0.0 6 94 2 204 116 0.0 6 112 4 205 107 0.0 6 104 2 0 0 0 1 206 121 0.0 6 116 5 207 157 0.0 6 153 4 208 150 0.0 6 146 4 209 141 0.0 6 137 4 210 76 0.0 6 74 2 211 52 0.0 6 51 1 212 64 0.0 6 62 2 213 99 0.0 6 97 2 214 69 0.0 6 68 1 215 66 0.0 6 62 4 216 140 0.0 6 132 7 1 217 127 0.0 6 125 2 218 82 0.0 6 80 2 219 108 0.0 6 104 4 220 72 0.0 6 68 4 221 93 0.0 6 92 1 222 91 0.0 6 90 1 223 57 0.0 6 56 1 224 47 0.0 6 45 1 1 225 119 0.0 6 116 3 226 153 0.0 6 149 4 227 132 0.0 6 131 1 228 129 0.0 6 126 3 229 121 0.0 6 118 2 1 230 92 0.0 6 90 2 231 84 0.0 6 78 6 232 63 0.0 6 62 0 1 233 80 0.0 6 79 1 234 84 0.0 6 83 1 235 66 0.0 6 62 4 236 58 0.0 6 57 1 237 106 0.0 6 105 1 238 90 0.0 6 89 0 1 239 145 0.0 6 139 6 240 205 0.0 6 198 7 241 242 0.0 6 234 8 242 229 0.0 6 219 10 243 192 0.0 6 184 6 0 2 244 166 0.0 6 163 3 245 205 0.0 6 195 10 246 68 0.0 6 67 1 247 83 0.0 6 78 5 248 103 0.0 6 101 2 249 183 0.0 6 177 4 2 250 147 0.0 6 142 4 1 251 148 0.0 6 143 5 252 102 0.0 6 95 6 1 253 131 0.0 6 126 5 254 172 0.0 6 165 7 255 124 0.0 6 120 4 256 64 0.0 6 64 257 90 0.0 6 87 2 1 258 154 0.0 6 150 4 259 199 0.0 6 191 8 260 176 0.0 6 172 4 261 104 0.0 6 101 3 262 187 0.0 6 183 4 263 285 0.0 6 276 7 2 264 122 0.0 6 118 4 265 113 0.0 6 108 5 266 92 0.0 6 88 4 267 150 0.0 6 142 7 0 0 1 268 137 0.0 6 133 4 269 162 0.0 6 157 4 1 270 124 0.0 6 112 11 1 271 315 0.0 6 307 7 1 272 386 0.0 6 373 13 273 270 0.0 6 259 7 2 1 1 274 266 0.0 6 257 9 275 235 0.0 6 221 14 276 835 0.0 6 806 28 0 1 277 782 0.0 6 766 15 0 1 278 525 0.0 6 498 27 279 276 0.0 6 229 42 5 280 32092 0.0 6 31454 629 6 3 281 102 0.0 6 95 6 1 285 2 0.0 6 1 1 286 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 15 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 1 0.0 6 1 71 1 0.0 6 1 72 1 0.0 6 1 76 3 0.0 6 2 1 78 1 0.0 6 1 88 1 0.0 6 1 97 1 0.0 6 1 98 2 0.0 6 2 113 1 0.0 6 1 172 1 0.0 6 1 176 1 0.0 6 1 180 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 47186 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 184.5 1 1 11 2 0.0 3 2 12 5 0.0 3 4 1 13 7 0.0 3 6 1 14 5 0.0 4 4 1 16 4 0.0 4 3 1 17 2 0.0 5 2 18 4 0.0 5 4 19 7 0.0 5 7 20 27 0.0 6 26 1 21 27 0.0 6 26 0 0 0 1 22 18 0.0 6 17 1 23 14 0.0 6 13 1 24 9 0.0 6 8 1 25 14 0.0 6 13 1 26 4 0.0 6 2 1 0 0 0 1 27 9 0.0 6 9 28 4 0.0 6 4 29 9 0.0 6 8 1 30 21 0.0 6 18 3 31 7 0.0 6 7 32 6 0.0 6 6 33 12 0.0 6 12 34 6 0.0 6 6 35 5 0.0 6 5 36 5 0.0 6 4 1 37 9 0.0 6 7 2 38 12 0.0 6 11 1 39 9 0.0 6 9 40 8 0.0 6 8 41 3 0.0 6 3 42 4 0.0 6 4 43 4 0.0 6 4 44 11 0.0 6 11 45 6 0.0 6 6 46 11 0.0 6 10 0 1 47 9 0.0 6 9 48 8 0.0 6 8 49 7 0.0 6 6 1 50 12 0.0 6 10 2 51 5 0.0 6 5 52 9 0.0 6 8 1 53 7 0.0 6 6 1 54 13 0.0 6 11 2 55 17 0.0 6 13 4 56 10 0.0 6 9 1 57 10 0.0 6 7 3 58 6 0.0 6 6 59 14 0.0 6 14 60 5 0.0 6 4 1 61 3 0.0 6 2 1 62 7 0.0 6 7 63 2 0.0 6 2 64 9 0.0 6 8 1 65 4 0.0 6 4 66 15 0.0 6 15 67 13 0.0 6 11 2 68 22 0.0 6 18 3 1 69 78 0.0 6 74 4 70 20 0.0 6 18 2 71 43 0.0 6 41 2 72 24 0.0 6 24 73 14 0.0 6 14 74 13 0.0 6 12 1 75 9 0.0 6 8 0 0 0 0 1 76 17 0.0 6 13 4 77 16 0.0 6 15 1 78 17 0.0 6 15 2 79 2 0.0 6 2 80 21 0.0 6 19 2 81 12 0.0 6 12 82 10 0.0 6 8 2 83 23 0.0 6 22 1 84 31 0.0 6 29 2 85 21 0.0 6 21 86 18 0.0 6 15 2 0 0 1 87 28 0.0 6 23 5 88 7 0.0 6 7 89 31 0.0 6 27 4 90 18 0.0 6 17 1 91 15 0.0 6 13 1 1 92 11 0.0 6 9 2 93 33 0.0 6 30 1 2 94 61 0.0 6 56 5 95 17 0.0 6 16 1 96 26 0.0 6 23 3 97 33 0.0 6 29 4 98 20 0.0 6 17 2 1 99 27 0.0 6 26 1 100 28 0.0 6 23 5 101 29 0.0 6 28 1 102 53 0.0 6 50 2 1 103 54 0.0 6 51 3 104 53 0.0 6 48 3 1 0 1 105 49 0.0 6 46 3 106 36 0.0 6 33 3 107 48 0.0 6 44 4 108 35 0.0 6 35 109 38 0.0 6 37 1 110 37 0.0 6 35 2 111 30 0.0 6 29 1 112 29 0.0 6 26 2 1 113 21 0.0 6 21 114 47 0.0 6 43 4 115 57 0.0 6 55 1 0 0 1 116 38 0.0 6 37 1 117 36 0.0 6 33 3 118 58 0.0 6 56 2 119 61 0.0 6 58 3 120 75 0.0 6 68 6 0 0 1 121 39 0.0 6 36 3 122 45 0.0 6 44 1 123 43 0.0 6 40 0 2 0 1 124 46 0.0 6 44 1 0 0 1 125 29 0.0 6 27 1 1 126 29 0.0 6 27 2 127 66 0.0 6 66 128 36 0.0 6 34 2 129 31 0.0 6 30 0 0 1 130 36 0.0 6 35 1 131 85 0.0 6 83 2 132 59 0.0 6 58 1 133 52 0.0 6 52 134 92 0.0 6 88 4 135 180 0.0 6 172 8 136 89 0.0 6 86 3 137 109 0.0 6 105 2 1 0 1 138 60 0.0 6 58 2 139 127 0.0 6 122 5 140 167 0.0 6 162 5 141 162 0.0 6 155 5 1 0 1 142 183 0.0 6 176 7 143 142 0.0 6 133 9 144 235 0.0 6 222 13 145 158 0.0 6 151 5 1 0 1 146 146 0.0 6 141 5 147 80 0.0 6 77 3 148 112 0.0 6 105 7 149 185 0.0 6 182 3 150 278 0.0 6 272 4 2 151 143 0.0 6 136 7 152 396 0.0 6 383 12 0 0 1 153 187 0.0 6 180 7 154 246 0.0 6 238 7 0 0 1 155 254 0.0 6 250 4 156 158 0.0 6 153 5 157 229 0.0 6 220 9 158 279 0.0 6 271 8 159 141 0.0 6 136 5 160 258 0.0 6 248 10 161 140 0.0 6 136 3 1 162 122 0.0 6 117 3 1 1 163 274 0.0 6 263 8 2 0 1 164 225 0.0 6 217 7 1 165 302 0.0 6 294 7 0 0 1 166 334 0.0 6 325 8 1 167 186 0.0 6 178 7 1 168 339 0.0 6 334 4 1 169 112 0.0 6 108 4 170 174 0.0 6 167 6 0 1 171 298 0.0 6 287 11 172 165 0.0 6 160 5 173 282 0.0 6 275 6 1 174 384 0.0 6 370 12 2 175 673 0.0 6 652 20 1 176 443 0.0 6 431 10 2 177 469 0.0 6 456 12 0 0 0 0 1 178 394 0.0 6 381 10 2 0 1 179 410 0.0 6 395 15 180 435 0.0 6 424 11 181 464 0.0 6 451 13 182 170 0.0 6 169 1 183 534 0.0 6 523 10 0 1 184 73 0.0 6 70 2 0 1 185 180 0.0 6 172 7 0 1 186 1066 0.0 6 1037 27 1 1 187 928 0.0 6 891 37 188 990 0.0 6 964 25 1 189 789 0.0 6 773 12 1 1 1 1 190 1409 0.0 6 1371 34 3 1 191 1090 0.0 6 1061 28 1 192 714 0.0 6 690 24 193 553 0.0 6 526 25 1 0 0 1 194 842 0.0 6 816 24 1 0 1 195 1148 0.0 6 1106 42 196 1971 0.0 6 1920 49 1 0 1 197 510 0.0 6 497 12 0 1 198 1712 0.0 6 1663 46 2 1 199 2426 0.0 6 2349 72 3 0 2 200 850 0.0 6 817 32 0 0 1 201 1115 0.0 6 1093 21 0 1 202 627 0.0 6 616 11 203 539 0.0 6 526 12 1 204 1059 0.0 6 1035 23 0 0 1 205 205 0.0 6 199 6 206 280 0.0 6 272 7 0 0 0 1 207 154 0.0 6 145 9 208 357 0.0 6 348 7 2 209 357 0.0 6 349 8 210 363 0.0 6 347 14 1 0 0 1 211 530 0.0 6 506 22 0 0 2 212 596 0.0 6 581 15 213 284 0.0 6 270 14 214 935 0.0 6 918 16 1 215 460 0.0 6 445 15 216 667 0.0 6 645 21 0 0 1 217 489 0.0 6 472 15 1 0 1 218 343 0.0 6 326 16 1 219 327 0.0 6 320 7 220 151 0.0 6 140 11 221 310 0.0 6 303 7 222 88 0.0 6 83 5 223 315 0.0 6 309 6 224 374 0.0 6 366 8 225 542 0.0 6 525 16 1 226 496 0.0 6 486 8 2 227 309 0.0 6 300 8 1 228 128 0.0 6 125 3 229 343 0.0 6 336 6 0 0 1 230 176 0.0 6 171 5 231 260 0.0 6 251 9 232 246 0.0 6 237 9 233 372 0.0 6 363 8 1 234 162 0.0 6 157 5 235 66 0.0 6 66 236 70 0.0 6 68 2 237 45 0.0 6 44 0 0 0 1 238 20 0.0 6 20 239 17 0.0 6 16 1 240 106 0.0 6 106 241 49 0.0 6 47 2 242 95 0.0 6 93 2 243 236 0.0 6 228 7 0 0 1 244 76 0.0 6 74 2 245 49 0.0 6 47 1 0 0 1 246 65 0.0 6 63 2 247 16 0.0 6 16 248 41 0.0 6 40 1 249 35 0.0 6 35 250 32 0.0 6 30 2 251 19 0.0 6 18 1 252 5 0.0 6 5 253 28 0.0 6 27 1 254 6 0.0 6 6 255 4 0.0 6 4 256 6 0.0 6 6 257 3 0.0 6 3 258 1 0.0 6 1 260 1 0.0 6 1 279 2 0.0 6 2 280 3 0.0 6 3 281 13 0.0 6 12 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 9 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 1 0.0 6 1 70 1 0.0 6 1 75 1 0.0 6 1 76 1 0.0 6 1 114 1 0.0 6 1 120 1 0.0 6 1 122 1 0.0 6 1 174 1 0.0 6 1 178 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_07_164_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_07_391_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_07_164_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_07_391_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.30 s (37896 µs/read; 0.00 M reads/minute). === Summary === Total read pairs processed: 8 Read 1 with adapter: 8 (100.0%) Read 2 with adapter: 8 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 0 (0.0%) Pairs written (passing filters): 8 (100.0%) Total basepairs processed: 3,490 bp Read 1: 1,905 bp Read 2: 1,585 bp Total written (filtered): 495 bp (14.2%) Read 1: 240 bp Read 2: 255 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 8 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 92 1 0.0 6 1 154 1 0.0 6 1 186 1 0.0 6 1 198 1 0.0 6 1 245 1 0.0 6 1 257 1 0.0 6 1 259 1 0.0 6 0 1 274 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 0 times === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 8 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 24 1 0.0 6 1 149 1 0.0 6 1 172 1 0.0 6 1 181 1 0.0 6 1 191 1 0.0 6 1 193 1 0.0 6 1 205 1 0.0 6 1 215 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 0 times This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_08_165_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_08_392_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_08_165_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_08_392_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.59 s (40 µs/read; 1.52 M reads/minute). === Summary === Total read pairs processed: 40,225 Read 1 with adapter: 40,219 (100.0%) Read 2 with adapter: 40,225 (100.0%) == Read fate breakdown == Pairs that were too short: 9 (0.0%) Pairs discarded as untrimmed: 6 (0.0%) Pairs written (passing filters): 40,210 (100.0%) Total basepairs processed: 18,203,659 bp Read 1: 10,225,717 bp Read 2: 7,977,942 bp Total written (filtered): 2,497,615 bp (13.7%) Read 1: 1,233,640 bp Read 2: 1,263,975 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 39763 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 628.5 0 0 2 4 1 157.1 1 0 1 6 3 9.8 1 0 0 3 9 1 0.2 2 1 14 1 0.0 4 1 15 1 0.0 4 1 16 1 0.0 4 1 17 1 0.0 5 0 1 19 2 0.0 5 2 20 25 0.0 6 23 2 21 14 0.0 6 14 22 9 0.0 6 9 23 8 0.0 6 8 24 8 0.0 6 8 25 5 0.0 6 5 26 3 0.0 6 3 27 3 0.0 6 3 28 6 0.0 6 6 29 4 0.0 6 4 30 3 0.0 6 3 31 5 0.0 6 5 32 3 0.0 6 3 33 2 0.0 6 2 34 4 0.0 6 4 35 3 0.0 6 3 36 1 0.0 6 1 37 3 0.0 6 3 38 2 0.0 6 2 39 4 0.0 6 3 0 1 40 2 0.0 6 2 41 3 0.0 6 3 43 2 0.0 6 2 44 1 0.0 6 1 45 4 0.0 6 4 46 3 0.0 6 2 1 47 1 0.0 6 1 48 1 0.0 6 1 49 3 0.0 6 3 50 3 0.0 6 3 51 1 0.0 6 1 52 4 0.0 6 4 53 4 0.0 6 4 54 1 0.0 6 1 55 3 0.0 6 3 56 2 0.0 6 2 57 2 0.0 6 2 58 3 0.0 6 3 60 3 0.0 6 2 1 61 2 0.0 6 2 62 5 0.0 6 5 63 4 0.0 6 3 1 65 1 0.0 6 1 66 1 0.0 6 1 67 5 0.0 6 5 68 5 0.0 6 4 1 69 4 0.0 6 3 1 70 3 0.0 6 3 72 3 0.0 6 3 73 3 0.0 6 2 1 74 1 0.0 6 0 1 75 1 0.0 6 1 76 1 0.0 6 1 77 3 0.0 6 3 78 3 0.0 6 3 79 4 0.0 6 4 80 5 0.0 6 5 81 4 0.0 6 4 82 2 0.0 6 2 83 3 0.0 6 3 84 3 0.0 6 3 85 5 0.0 6 5 86 4 0.0 6 4 87 6 0.0 6 6 88 3 0.0 6 3 89 1 0.0 6 1 90 4 0.0 6 4 91 2 0.0 6 2 92 6 0.0 6 6 93 1 0.0 6 1 94 3 0.0 6 3 95 1 0.0 6 1 96 6 0.0 6 6 97 1 0.0 6 1 98 3 0.0 6 3 99 5 0.0 6 4 1 101 13 0.0 6 13 102 4 0.0 6 4 103 2 0.0 6 2 104 8 0.0 6 8 105 6 0.0 6 6 106 10 0.0 6 10 107 12 0.0 6 11 1 108 13 0.0 6 13 109 9 0.0 6 9 110 11 0.0 6 11 111 8 0.0 6 8 112 10 0.0 6 10 113 10 0.0 6 10 114 20 0.0 6 20 115 9 0.0 6 8 1 116 12 0.0 6 12 117 11 0.0 6 11 118 11 0.0 6 11 119 15 0.0 6 15 120 11 0.0 6 11 121 8 0.0 6 8 122 11 0.0 6 10 1 123 14 0.0 6 14 124 17 0.0 6 17 125 17 0.0 6 17 126 13 0.0 6 13 127 13 0.0 6 12 0 0 0 0 0 1 128 18 0.0 6 18 129 17 0.0 6 17 130 19 0.0 6 18 1 131 11 0.0 6 11 132 19 0.0 6 19 133 18 0.0 6 18 134 22 0.0 6 22 135 13 0.0 6 13 136 22 0.0 6 20 2 137 22 0.0 6 22 138 30 0.0 6 29 1 139 23 0.0 6 23 140 44 0.0 6 43 1 141 42 0.0 6 41 1 142 115 0.0 6 115 143 246 0.0 6 246 144 264 0.0 6 263 1 145 274 0.0 6 274 146 295 0.0 6 294 1 147 343 0.0 6 342 1 148 320 0.0 6 319 1 149 424 0.0 6 422 2 150 364 0.0 6 363 0 0 1 151 399 0.0 6 399 152 426 0.0 6 426 153 252 0.0 6 251 1 154 236 0.0 6 236 155 215 0.0 6 214 1 156 193 0.0 6 193 157 151 0.0 6 151 158 165 0.0 6 165 159 174 0.0 6 173 1 160 191 0.0 6 191 161 235 0.0 6 235 162 243 0.0 6 242 1 163 221 0.0 6 220 1 164 203 0.0 6 203 165 167 0.0 6 167 166 159 0.0 6 157 2 167 139 0.0 6 139 168 124 0.0 6 124 169 154 0.0 6 154 170 132 0.0 6 132 171 146 0.0 6 145 1 172 189 0.0 6 189 173 177 0.0 6 176 1 174 158 0.0 6 158 175 212 0.0 6 211 1 176 146 0.0 6 146 177 161 0.0 6 159 2 178 129 0.0 6 129 179 108 0.0 6 107 1 180 200 0.0 6 198 2 181 122 0.0 6 121 1 182 128 0.0 6 128 183 140 0.0 6 139 1 184 139 0.0 6 137 2 185 168 0.0 6 168 186 178 0.0 6 175 3 187 146 0.0 6 144 2 188 137 0.0 6 136 1 189 145 0.0 6 142 2 1 190 157 0.0 6 154 3 191 154 0.0 6 152 2 192 153 0.0 6 153 193 246 0.0 6 246 194 198 0.0 6 197 1 195 159 0.0 6 156 3 196 171 0.0 6 168 3 197 218 0.0 6 211 7 198 253 0.0 6 248 4 1 199 223 0.0 6 218 5 200 280 0.0 6 279 1 201 231 0.0 6 227 3 1 202 233 0.0 6 230 3 203 244 0.0 6 240 4 204 230 0.0 6 224 6 205 247 0.0 6 245 2 206 231 0.0 6 229 2 207 259 0.0 6 256 3 208 292 0.0 6 290 2 209 240 0.0 6 236 4 210 242 0.0 6 240 2 211 210 0.0 6 207 3 212 165 0.0 6 164 1 213 229 0.0 6 227 2 214 238 0.0 6 235 3 215 213 0.0 6 211 2 216 234 0.0 6 234 217 328 0.0 6 326 2 218 240 0.0 6 236 4 219 224 0.0 6 221 3 220 243 0.0 6 242 1 221 201 0.0 6 199 2 222 198 0.0 6 194 4 223 203 0.0 6 202 1 224 200 0.0 6 197 3 225 238 0.0 6 233 4 1 226 279 0.0 6 277 2 227 306 0.0 6 299 7 228 263 0.0 6 256 6 1 229 162 0.0 6 156 6 230 222 0.0 6 219 3 231 195 0.0 6 193 2 232 207 0.0 6 205 2 233 262 0.0 6 262 234 225 0.0 6 225 235 184 0.0 6 181 3 236 223 0.0 6 220 3 237 203 0.0 6 200 3 238 246 0.0 6 240 6 239 178 0.0 6 172 6 240 260 0.0 6 257 3 241 257 0.0 6 251 6 242 259 0.0 6 255 4 243 184 0.0 6 181 3 244 203 0.0 6 199 4 245 239 0.0 6 235 4 246 141 0.0 6 138 3 247 166 0.0 6 163 3 248 145 0.0 6 143 2 249 192 0.0 6 190 2 250 153 0.0 6 149 4 251 189 0.0 6 186 3 252 179 0.0 6 174 5 253 121 0.0 6 114 7 254 226 0.0 6 223 3 255 143 0.0 6 141 2 256 194 0.0 6 192 2 257 153 0.0 6 150 3 258 138 0.0 6 135 3 259 325 0.0 6 313 12 260 297 0.0 6 292 5 261 182 0.0 6 173 9 262 170 0.0 6 162 8 263 237 0.0 6 232 5 264 167 0.0 6 160 7 265 214 0.0 6 210 3 1 266 132 0.0 6 130 2 267 145 0.0 6 142 3 268 175 0.0 6 166 8 0 0 1 269 218 0.0 6 210 8 270 117 0.0 6 112 5 271 206 0.0 6 200 6 272 269 0.0 6 262 7 273 193 0.0 6 188 4 1 274 154 0.0 6 151 2 1 275 255 0.0 6 248 6 1 276 314 0.0 6 309 5 277 400 0.0 6 392 8 278 369 0.0 6 364 5 279 674 0.0 6 668 6 280 1028 0.0 6 998 30 281 8131 0.0 6 8026 98 6 1 282 5 0.0 6 4 1 283 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 456 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 1 0.0 6 0 1 61 49 0.0 6 47 2 62 2 0.0 6 1 1 69 13 0.0 6 13 70 2 0.0 6 2 71 2 0.0 6 2 72 4 0.0 6 4 73 4 0.0 6 4 74 11 0.0 6 11 75 5 0.0 6 5 76 10 0.0 6 10 77 9 0.0 6 9 78 5 0.0 6 5 79 7 0.0 6 7 80 19 0.0 6 19 81 6 0.0 6 6 83 2 0.0 6 2 84 8 0.0 6 8 85 1 0.0 6 1 86 1 0.0 6 1 87 5 0.0 6 5 88 6 0.0 6 6 89 6 0.0 6 6 90 5 0.0 6 5 91 10 0.0 6 10 92 6 0.0 6 6 93 4 0.0 6 4 94 4 0.0 6 4 96 3 0.0 6 3 97 1 0.0 6 1 98 1 0.0 6 1 99 1 0.0 6 1 100 2 0.0 6 2 101 2 0.0 6 2 102 4 0.0 6 4 103 6 0.0 6 6 104 1 0.0 6 1 105 6 0.0 6 6 107 3 0.0 6 3 108 1 0.0 6 1 109 5 0.0 6 5 110 2 0.0 6 2 111 5 0.0 6 5 112 4 0.0 6 4 113 2 0.0 6 2 114 2 0.0 6 2 115 2 0.0 6 2 118 3 0.0 6 3 119 5 0.0 6 5 120 3 0.0 6 3 121 2 0.0 6 2 124 3 0.0 6 3 125 2 0.0 6 2 126 5 0.0 6 5 127 5 0.0 6 5 129 1 0.0 6 1 130 2 0.0 6 2 131 1 0.0 6 1 132 3 0.0 6 3 133 4 0.0 6 4 134 3 0.0 6 3 135 1 0.0 6 1 136 2 0.0 6 1 0 0 0 0 0 1 137 1 0.0 6 1 138 1 0.0 6 1 139 2 0.0 6 2 141 2 0.0 6 2 143 1 0.0 6 1 145 3 0.0 6 3 146 3 0.0 6 3 147 3 0.0 6 3 148 1 0.0 6 1 150 1 0.0 6 1 152 3 0.0 6 3 153 1 0.0 6 1 154 2 0.0 6 2 156 5 0.0 6 5 157 1 0.0 6 1 158 2 0.0 6 2 159 3 0.0 6 3 160 1 0.0 6 1 162 2 0.0 6 2 163 2 0.0 6 2 164 3 0.0 6 3 165 1 0.0 6 1 166 5 0.0 6 5 167 3 0.0 6 3 168 1 0.0 6 1 169 1 0.0 6 1 170 1 0.0 6 1 171 2 0.0 6 2 172 3 0.0 6 3 173 3 0.0 6 3 175 3 0.0 6 3 176 1 0.0 6 1 177 2 0.0 6 2 178 1 0.0 6 1 180 2 0.0 6 2 182 2 0.0 6 2 184 3 0.0 6 3 185 3 0.0 6 3 186 5 0.0 6 5 187 4 0.0 6 4 189 2 0.0 6 2 190 2 0.0 6 2 192 1 0.0 6 1 193 1 0.0 6 1 194 2 0.0 6 2 196 2 0.0 6 2 199 1 0.0 6 1 200 1 0.0 6 1 201 3 0.0 6 3 202 2 0.0 6 2 204 3 0.0 6 3 206 1 0.0 6 1 207 2 0.0 6 2 208 2 0.0 6 2 211 1 0.0 6 1 212 1 0.0 6 1 213 2 0.0 6 2 215 2 0.0 6 2 216 1 0.0 6 1 217 3 0.0 6 3 218 2 0.0 6 2 219 2 0.0 6 2 220 3 0.0 6 3 221 3 0.0 6 3 223 1 0.0 6 1 226 1 0.0 6 1 227 1 0.0 6 1 228 1 0.0 6 1 229 2 0.0 6 2 230 1 0.0 6 1 236 1 0.0 6 1 237 1 0.0 6 1 238 1 0.0 6 1 240 2 0.0 6 2 266 1 0.0 6 1 274 1 0.0 6 1 278 1 0.0 6 1 290 1 0.0 6 1 293 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 39749 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 2.5 2 0 1 10 3 0.0 3 3 11 3 0.0 3 3 12 5 0.0 3 4 1 13 8 0.0 3 7 1 14 3 0.0 4 3 16 5 0.0 4 5 17 9 0.0 5 8 1 18 10 0.0 5 9 1 19 7 0.0 5 6 1 20 27 0.0 6 25 1 1 21 26 0.0 6 25 1 22 16 0.0 6 15 1 23 15 0.0 6 15 24 9 0.0 6 7 2 25 8 0.0 6 6 1 1 26 7 0.0 6 7 27 9 0.0 6 8 1 28 3 0.0 6 2 0 1 29 2 0.0 6 2 30 7 0.0 6 6 1 31 6 0.0 6 5 1 32 2 0.0 6 2 33 7 0.0 6 7 34 5 0.0 6 4 1 35 5 0.0 6 4 1 36 6 0.0 6 5 1 37 4 0.0 6 4 38 5 0.0 6 5 39 9 0.0 6 8 1 40 6 0.0 6 6 41 3 0.0 6 3 42 6 0.0 6 5 1 43 6 0.0 6 6 44 11 0.0 6 11 45 8 0.0 6 8 46 14 0.0 6 14 47 11 0.0 6 10 1 48 8 0.0 6 8 49 9 0.0 6 8 1 50 9 0.0 6 9 51 5 0.0 6 3 2 52 18 0.0 6 15 2 1 53 9 0.0 6 7 2 54 9 0.0 6 8 1 55 4 0.0 6 3 1 56 13 0.0 6 12 1 57 10 0.0 6 9 1 58 12 0.0 6 11 1 59 16 0.0 6 12 2 2 60 13 0.0 6 13 61 14 0.0 6 14 62 7 0.0 6 7 63 12 0.0 6 9 2 1 64 14 0.0 6 12 2 65 8 0.0 6 8 66 11 0.0 6 11 67 31 0.0 6 31 68 75 0.0 6 73 2 69 20 0.0 6 20 70 34 0.0 6 33 1 71 15 0.0 6 14 1 72 29 0.0 6 29 73 10 0.0 6 10 74 11 0.0 6 10 1 75 7 0.0 6 6 1 76 20 0.0 6 18 1 1 77 20 0.0 6 15 5 78 15 0.0 6 14 1 79 15 0.0 6 14 1 80 26 0.0 6 23 3 81 17 0.0 6 16 0 1 82 21 0.0 6 20 1 83 32 0.0 6 30 2 84 47 0.0 6 45 2 85 29 0.0 6 26 3 86 18 0.0 6 15 2 1 87 27 0.0 6 24 3 88 23 0.0 6 21 2 89 16 0.0 6 14 2 90 31 0.0 6 28 2 0 0 0 1 91 27 0.0 6 27 92 27 0.0 6 26 0 0 1 93 27 0.0 6 24 2 1 94 43 0.0 6 38 2 1 0 1 1 95 34 0.0 6 33 1 96 26 0.0 6 24 2 97 38 0.0 6 36 0 2 98 25 0.0 6 24 0 0 1 99 28 0.0 6 26 1 0 0 0 1 100 23 0.0 6 21 2 101 35 0.0 6 32 1 2 102 34 0.0 6 31 3 103 38 0.0 6 34 4 104 49 0.0 6 48 0 0 1 105 68 0.0 6 66 2 106 52 0.0 6 52 107 46 0.0 6 42 3 0 0 0 0 1 108 37 0.0 6 36 1 109 40 0.0 6 38 2 110 52 0.0 6 51 1 111 42 0.0 6 41 1 112 53 0.0 6 51 1 0 0 1 113 52 0.0 6 48 3 0 1 114 46 0.0 6 44 1 1 115 53 0.0 6 51 2 116 58 0.0 6 57 1 117 58 0.0 6 57 1 118 78 0.0 6 78 119 86 0.0 6 86 120 86 0.0 6 84 2 121 86 0.0 6 82 3 0 0 0 0 1 122 99 0.0 6 97 2 123 71 0.0 6 70 1 124 100 0.0 6 93 6 1 125 91 0.0 6 86 5 126 89 0.0 6 87 2 127 123 0.0 6 121 2 128 99 0.0 6 96 3 129 127 0.0 6 122 4 1 130 113 0.0 6 110 3 131 146 0.0 6 142 4 132 164 0.0 6 163 0 1 133 151 0.0 6 150 1 134 210 0.0 6 204 6 135 288 0.0 6 280 7 1 136 209 0.0 6 205 3 0 0 0 0 1 137 232 0.0 6 232 138 263 0.0 6 261 2 139 335 0.0 6 330 5 140 425 0.0 6 419 6 141 437 0.0 6 430 7 142 479 0.0 6 473 6 143 566 0.0 6 559 6 0 0 0 1 144 559 0.0 6 547 11 0 0 0 1 145 577 0.0 6 574 1 0 1 1 146 519 0.0 6 514 4 0 0 1 147 508 0.0 6 503 5 148 558 0.0 6 554 4 149 545 0.0 6 542 3 150 594 0.0 6 581 12 0 0 0 1 151 599 0.0 6 592 7 152 552 0.0 6 543 8 0 1 153 516 0.0 6 507 8 1 154 572 0.0 6 562 10 155 430 0.0 6 424 6 156 511 0.0 6 508 3 157 555 0.0 6 545 8 0 0 1 1 158 610 0.0 6 601 8 0 0 1 159 418 0.0 6 414 4 160 463 0.0 6 453 10 161 393 0.0 6 386 6 0 0 0 1 162 357 0.0 6 351 5 0 0 0 0 1 163 354 0.0 6 347 7 164 372 0.0 6 367 5 165 396 0.0 6 391 5 166 440 0.0 6 434 6 167 401 0.0 6 395 6 168 515 0.0 6 502 13 169 374 0.0 6 369 4 0 0 1 170 317 0.0 6 311 6 171 507 0.0 6 503 3 1 172 332 0.0 6 332 173 325 0.0 6 320 5 174 384 0.0 6 379 5 175 531 0.0 6 524 7 176 427 0.0 6 421 5 1 177 435 0.0 6 429 6 178 439 0.0 6 434 4 0 1 179 448 0.0 6 442 6 180 346 0.0 6 337 6 2 0 1 181 506 0.0 6 490 14 1 0 1 182 208 0.0 6 202 5 0 0 1 183 416 0.0 6 409 7 184 377 0.0 6 368 8 1 185 363 0.0 6 351 11 1 186 690 0.0 6 663 26 0 1 187 487 0.0 6 479 5 0 2 1 188 487 0.0 6 476 11 189 541 0.0 6 523 18 190 752 0.0 6 734 18 191 630 0.0 6 614 15 1 192 554 0.0 6 541 12 0 0 0 1 193 299 0.0 6 295 3 0 0 1 194 437 0.0 6 426 10 0 1 195 540 0.0 6 529 11 196 1131 0.0 6 1110 20 0 1 197 462 0.0 6 447 14 0 1 198 675 0.0 6 654 18 1 0 2 199 885 0.0 6 863 20 1 1 200 347 0.0 6 343 4 201 475 0.0 6 464 10 1 202 233 0.0 6 227 5 1 203 193 0.0 6 190 3 204 232 0.0 6 222 10 205 94 0.0 6 94 206 94 0.0 6 92 2 207 80 0.0 6 77 3 208 144 0.0 6 143 1 209 114 0.0 6 110 3 0 0 0 0 1 210 112 0.0 6 109 3 211 115 0.0 6 109 6 212 137 0.0 6 137 213 76 0.0 6 75 1 214 91 0.0 6 88 3 215 157 0.0 6 152 5 216 172 0.0 6 166 6 217 121 0.0 6 121 218 99 0.0 6 99 219 102 0.0 6 98 4 220 49 0.0 6 45 4 221 51 0.0 6 51 222 139 0.0 6 125 14 223 199 0.0 6 195 4 224 74 0.0 6 72 2 225 125 0.0 6 121 4 226 80 0.0 6 79 1 227 88 0.0 6 85 3 228 71 0.0 6 71 229 88 0.0 6 85 3 230 81 0.0 6 80 1 231 40 0.0 6 39 1 232 123 0.0 6 122 1 233 71 0.0 6 68 3 234 16 0.0 6 16 235 17 0.0 6 16 1 236 7 0.0 6 7 237 8 0.0 6 8 238 7 0.0 6 7 239 5 0.0 6 5 240 33 0.0 6 33 241 23 0.0 6 23 242 49 0.0 6 48 1 243 28 0.0 6 28 244 19 0.0 6 17 2 245 10 0.0 6 10 246 10 0.0 6 10 247 2 0.0 6 2 248 7 0.0 6 6 1 249 9 0.0 6 9 250 5 0.0 6 4 1 251 8 0.0 6 8 252 3 0.0 6 3 253 3 0.0 6 3 254 2 0.0 6 2 255 2 0.0 6 2 278 1 0.0 6 1 279 4 0.0 6 3 1 280 34 0.0 6 29 4 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 476 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 6 35 9.8 1 0 0 35 55 1 0.0 6 1 56 1 0.0 6 1 57 2 0.0 6 2 62 2 0.0 6 2 63 10 0.0 6 10 69 10 0.0 6 10 70 1 0.0 6 1 71 7 0.0 6 7 72 6 0.0 6 6 73 7 0.0 6 7 74 8 0.0 6 8 75 7 0.0 6 7 76 4 0.0 6 4 77 8 0.0 6 8 78 9 0.0 6 8 0 0 0 0 0 1 79 11 0.0 6 11 80 15 0.0 6 15 81 17 0.0 6 17 82 6 0.0 6 6 83 7 0.0 6 7 85 6 0.0 6 6 86 3 0.0 6 3 87 5 0.0 6 5 88 2 0.0 6 2 89 3 0.0 6 3 90 9 0.0 6 9 91 6 0.0 6 6 92 7 0.0 6 7 93 5 0.0 6 5 94 3 0.0 6 3 95 3 0.0 6 3 96 3 0.0 6 3 97 4 0.0 6 4 98 3 0.0 6 3 99 2 0.0 6 2 100 2 0.0 6 2 101 4 0.0 6 4 102 9 0.0 6 9 103 2 0.0 6 2 104 3 0.0 6 3 105 2 0.0 6 2 106 6 0.0 6 6 108 3 0.0 6 3 109 8 0.0 6 8 110 2 0.0 6 2 111 3 0.0 6 3 112 6 0.0 6 6 113 2 0.0 6 2 114 3 0.0 6 3 115 3 0.0 6 3 116 5 0.0 6 5 117 3 0.0 6 3 118 3 0.0 6 3 119 3 0.0 6 3 120 1 0.0 6 1 121 1 0.0 6 1 122 1 0.0 6 1 123 3 0.0 6 3 124 1 0.0 6 1 125 1 0.0 6 1 126 1 0.0 6 1 127 5 0.0 6 5 128 5 0.0 6 5 130 3 0.0 6 3 131 3 0.0 6 3 132 3 0.0 6 3 133 4 0.0 6 4 134 1 0.0 6 1 135 2 0.0 6 2 136 2 0.0 6 2 137 1 0.0 6 1 138 1 0.0 6 1 139 7 0.0 6 7 141 3 0.0 6 3 142 3 0.0 6 3 144 1 0.0 6 1 145 1 0.0 6 1 146 6 0.0 6 6 148 4 0.0 6 4 149 2 0.0 6 2 150 3 0.0 6 3 152 1 0.0 6 1 153 1 0.0 6 1 154 3 0.0 6 3 155 3 0.0 6 3 156 2 0.0 6 2 157 2 0.0 6 2 158 1 0.0 6 1 160 2 0.0 6 2 161 6 0.0 6 6 163 3 0.0 6 3 164 4 0.0 6 4 165 1 0.0 6 1 166 5 0.0 6 5 167 1 0.0 6 1 168 3 0.0 6 3 169 2 0.0 6 2 170 1 0.0 6 1 172 3 0.0 6 3 173 2 0.0 6 2 175 3 0.0 6 3 176 2 0.0 6 2 177 2 0.0 6 2 178 1 0.0 6 1 179 2 0.0 6 2 180 3 0.0 6 3 181 3 0.0 6 3 182 3 0.0 6 3 183 3 0.0 6 3 187 3 0.0 6 3 189 3 0.0 6 3 190 3 0.0 6 3 191 2 0.0 6 2 192 2 0.0 6 2 193 1 0.0 6 1 194 2 0.0 6 2 197 1 0.0 6 1 198 1 0.0 6 0 1 201 1 0.0 6 1 202 1 0.0 6 1 203 2 0.0 6 2 208 1 0.0 6 1 216 1 0.0 6 1 228 1 0.0 6 1 233 1 0.0 6 1 235 2 0.0 6 2 242 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_09_166_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_09_393_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_09_166_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_09_393_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.10 s (38 µs/read; 1.59 M reads/minute). === Summary === Total read pairs processed: 55,711 Read 1 with adapter: 55,710 (100.0%) Read 2 with adapter: 55,695 (100.0%) == Read fate breakdown == Pairs that were too short: 4 (0.0%) Pairs discarded as untrimmed: 17 (0.0%) Pairs written (passing filters): 55,690 (100.0%) Total basepairs processed: 26,495,655 bp Read 1: 14,961,015 bp Read 2: 11,534,640 bp Total written (filtered): 3,343,467 bp (12.6%) Read 1: 1,704,129 bp Read 2: 1,639,338 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 55174 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 870.5 0 1 4 1 217.6 1 1 5 1 54.4 1 1 6 1 13.6 1 0 0 1 10 2 0.1 3 2 14 1 0.0 4 1 15 1 0.0 4 1 16 2 0.0 4 1 1 17 1 0.0 5 1 19 1 0.0 5 1 20 26 0.0 6 26 21 7 0.0 6 7 22 12 0.0 6 10 2 23 9 0.0 6 8 1 24 8 0.0 6 8 25 11 0.0 6 10 1 26 6 0.0 6 6 27 3 0.0 6 2 1 28 1 0.0 6 1 29 3 0.0 6 3 30 3 0.0 6 3 31 3 0.0 6 3 32 2 0.0 6 2 33 3 0.0 6 3 34 2 0.0 6 2 35 4 0.0 6 4 36 5 0.0 6 5 37 3 0.0 6 3 38 3 0.0 6 1 2 40 2 0.0 6 2 41 3 0.0 6 3 43 2 0.0 6 2 44 2 0.0 6 2 45 1 0.0 6 0 1 46 2 0.0 6 2 47 2 0.0 6 2 48 2 0.0 6 1 0 0 0 0 1 49 1 0.0 6 1 50 1 0.0 6 0 0 0 0 0 0 1 51 2 0.0 6 2 52 3 0.0 6 3 53 2 0.0 6 2 54 3 0.0 6 3 56 3 0.0 6 3 57 1 0.0 6 1 58 1 0.0 6 1 59 2 0.0 6 2 60 2 0.0 6 2 61 4 0.0 6 4 62 2 0.0 6 2 63 2 0.0 6 2 64 2 0.0 6 2 65 3 0.0 6 3 66 3 0.0 6 3 67 8 0.0 6 8 68 1 0.0 6 1 69 1 0.0 6 1 70 1 0.0 6 1 72 5 0.0 6 3 1 0 0 0 1 73 1 0.0 6 1 74 1 0.0 6 1 75 3 0.0 6 3 76 2 0.0 6 2 77 3 0.0 6 1 2 78 7 0.0 6 7 79 4 0.0 6 4 80 3 0.0 6 3 82 1 0.0 6 1 83 4 0.0 6 4 84 2 0.0 6 2 85 4 0.0 6 4 86 5 0.0 6 5 87 5 0.0 6 5 88 2 0.0 6 2 89 3 0.0 6 3 91 6 0.0 6 6 92 4 0.0 6 4 95 1 0.0 6 1 96 4 0.0 6 4 97 7 0.0 6 7 98 7 0.0 6 6 1 99 8 0.0 6 6 2 100 7 0.0 6 7 101 12 0.0 6 12 102 8 0.0 6 8 103 6 0.0 6 6 104 8 0.0 6 6 1 1 105 5 0.0 6 5 106 9 0.0 6 9 107 8 0.0 6 7 1 108 9 0.0 6 8 1 109 11 0.0 6 11 110 9 0.0 6 9 111 5 0.0 6 5 112 8 0.0 6 8 113 6 0.0 6 6 114 6 0.0 6 6 115 12 0.0 6 12 116 10 0.0 6 10 117 15 0.0 6 14 1 118 5 0.0 6 5 119 6 0.0 6 6 120 4 0.0 6 4 121 6 0.0 6 5 1 122 16 0.0 6 15 1 123 11 0.0 6 10 0 0 0 0 0 1 124 11 0.0 6 11 125 16 0.0 6 15 1 126 8 0.0 6 7 1 127 13 0.0 6 13 128 19 0.0 6 18 1 129 13 0.0 6 11 1 0 0 0 0 1 130 18 0.0 6 16 1 0 0 0 1 131 10 0.0 6 10 132 19 0.0 6 19 133 34 0.0 6 34 134 19 0.0 6 19 135 26 0.0 6 26 136 32 0.0 6 31 1 137 33 0.0 6 32 0 1 138 30 0.0 6 29 1 139 34 0.0 6 34 140 352 0.0 6 352 141 126 0.0 6 126 142 100 0.0 6 100 143 189 0.0 6 188 1 144 80 0.0 6 80 145 204 0.0 6 202 2 146 97 0.0 6 96 1 147 42 0.0 6 42 148 133 0.0 6 132 1 149 78 0.0 6 76 2 150 255 0.0 6 254 1 151 235 0.0 6 235 152 69 0.0 6 69 153 215 0.0 6 215 154 72 0.0 6 72 155 208 0.0 6 208 156 72 0.0 6 72 157 36 0.0 6 36 158 59 0.0 6 58 1 159 70 0.0 6 70 160 516 0.0 6 512 3 0 0 0 1 161 128 0.0 6 127 1 162 308 0.0 6 307 1 163 339 0.0 6 336 2 1 164 128 0.0 6 125 3 165 124 0.0 6 124 166 574 0.0 6 573 1 167 499 0.0 6 498 1 168 746 0.0 6 745 1 169 757 0.0 6 757 170 97 0.0 6 97 171 31 0.0 6 31 172 35 0.0 6 34 1 173 43 0.0 6 42 1 174 65 0.0 6 65 175 68 0.0 6 66 2 176 93 0.0 6 89 4 177 721 0.0 6 719 2 178 137 0.0 6 134 3 179 73 0.0 6 72 1 180 61 0.0 6 61 181 68 0.0 6 68 182 289 0.0 6 287 2 183 107 0.0 6 105 2 184 203 0.0 6 198 5 185 122 0.0 6 119 3 186 112 0.0 6 108 4 187 102 0.0 6 100 1 1 188 199 0.0 6 197 2 189 108 0.0 6 106 2 190 125 0.0 6 124 1 191 269 0.0 6 266 3 192 120 0.0 6 119 1 193 171 0.0 6 167 4 194 543 0.0 6 539 4 195 138 0.0 6 137 1 196 115 0.0 6 115 197 140 0.0 6 135 5 198 166 0.0 6 164 2 199 262 0.0 6 258 3 1 200 458 0.0 6 451 5 2 201 1011 0.0 6 1006 5 202 231 0.0 6 223 8 203 158 0.0 6 153 5 204 144 0.0 6 138 5 1 205 202 0.0 6 197 5 206 162 0.0 6 156 6 207 227 0.0 6 221 5 1 208 290 0.0 6 285 5 209 260 0.0 6 254 6 210 272 0.0 6 270 2 211 251 0.0 6 247 3 0 0 0 0 1 212 689 0.0 6 687 2 213 162 0.0 6 157 5 214 163 0.0 6 157 6 215 138 0.0 6 136 2 216 199 0.0 6 190 9 217 315 0.0 6 307 8 218 128 0.0 6 120 7 0 0 0 1 219 150 0.0 6 143 6 1 220 120 0.0 6 115 4 1 221 145 0.0 6 140 5 222 171 0.0 6 163 7 1 223 140 0.0 6 134 6 224 137 0.0 6 134 3 225 170 0.0 6 166 4 226 297 0.0 6 282 13 1 0 1 227 305 0.0 6 292 12 0 1 228 337 0.0 6 330 6 1 229 234 0.0 6 226 8 230 257 0.0 6 251 6 231 208 0.0 6 205 3 232 248 0.0 6 245 3 233 338 0.0 6 332 5 0 1 234 248 0.0 6 238 10 235 226 0.0 6 219 7 236 239 0.0 6 230 7 1 0 1 237 332 0.0 6 324 8 238 215 0.0 6 207 8 239 192 0.0 6 182 10 240 246 0.0 6 234 11 1 241 319 0.0 6 305 14 242 281 0.0 6 275 6 243 205 0.0 6 199 6 244 198 0.0 6 187 11 245 264 0.0 6 250 14 246 140 0.0 6 134 6 247 154 0.0 6 150 4 248 166 0.0 6 156 10 249 296 0.0 6 286 10 250 196 0.0 6 190 6 251 255 0.0 6 247 6 2 252 241 0.0 6 230 11 253 204 0.0 6 193 11 254 292 0.0 6 282 9 1 255 185 0.0 6 179 6 256 244 0.0 6 231 13 257 219 0.0 6 214 5 258 270 0.0 6 257 12 1 259 424 0.0 6 408 15 1 260 442 0.0 6 431 10 1 261 219 0.0 6 206 12 1 262 326 0.0 6 303 23 263 374 0.0 6 366 8 264 252 0.0 6 242 9 1 265 345 0.0 6 334 10 0 1 266 237 0.0 6 225 12 267 250 0.0 6 237 13 268 301 0.0 6 286 14 1 269 341 0.0 6 336 4 1 270 229 0.0 6 219 10 271 457 0.0 6 433 23 1 272 529 0.0 6 511 16 2 273 343 0.0 6 336 7 274 392 0.0 6 381 11 275 419 0.0 6 407 11 1 276 814 0.0 6 789 24 1 277 840 0.0 6 813 27 278 667 0.0 6 637 29 1 279 1489 0.0 6 1449 38 2 280 2726 0.0 6 2598 119 6 3 281 15786 0.0 6 15449 331 5 0 1 282 10 0.0 6 7 2 0 1 283 2 0.0 6 1 0 0 0 0 1 284 2 0.0 6 0 0 1 0 1 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 536 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 2 0.0 6 2 69 6 0.0 6 6 70 14 0.0 6 14 71 1 0.0 6 1 72 3 0.0 6 3 73 4 0.0 6 4 74 3 0.0 6 3 75 5 0.0 6 5 76 2 0.0 6 2 77 1 0.0 6 1 78 4 0.0 6 4 79 5 0.0 6 5 80 7 0.0 6 7 81 9 0.0 6 9 82 6 0.0 6 6 83 5 0.0 6 5 84 3 0.0 6 3 85 3 0.0 6 3 88 1 0.0 6 1 89 8 0.0 6 8 90 23 0.0 6 23 91 5 0.0 6 5 92 11 0.0 6 11 93 7 0.0 6 7 95 8 0.0 6 8 96 23 0.0 6 23 97 19 0.0 6 18 0 0 0 0 0 1 98 27 0.0 6 27 99 24 0.0 6 24 100 1 0.0 6 1 106 15 0.0 6 15 107 23 0.0 6 23 108 1 0.0 6 1 110 1 0.0 6 1 111 6 0.0 6 6 112 10 0.0 6 10 113 1 0.0 6 1 114 9 0.0 6 9 117 1 0.0 6 1 118 2 0.0 6 2 119 1 0.0 6 1 120 5 0.0 6 5 121 5 0.0 6 5 123 7 0.0 6 7 124 21 0.0 6 21 125 1 0.0 6 1 129 1 0.0 6 1 130 16 0.0 6 16 131 39 0.0 6 39 137 3 0.0 6 3 141 15 0.0 6 15 142 34 0.0 6 34 143 2 0.0 6 2 146 3 0.0 6 3 147 3 0.0 6 3 156 3 0.0 6 3 157 6 0.0 6 5 1 161 1 0.0 6 1 162 6 0.0 6 6 163 3 0.0 6 3 187 1 0.0 6 0 1 197 1 0.0 6 1 198 2 0.0 6 2 199 14 0.0 6 14 200 24 0.0 6 24 201 1 0.0 6 1 211 2 0.0 6 1 1 212 4 0.0 6 4 222 1 0.0 6 1 250 1 0.0 6 1 273 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 55168 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 13.6 1 0 1 7 3 3.4 2 1 2 9 1 0.2 2 1 10 3 0.1 3 3 11 3 0.0 3 3 12 2 0.0 3 2 13 4 0.0 3 3 1 14 5 0.0 4 5 15 1 0.0 4 1 16 4 0.0 4 3 1 17 11 0.0 5 9 2 18 2 0.0 5 2 19 36 0.0 5 36 20 79 0.0 6 75 4 21 43 0.0 6 42 1 22 35 0.0 6 35 23 22 0.0 6 21 0 1 24 12 0.0 6 10 2 25 12 0.0 6 12 26 6 0.0 6 6 27 11 0.0 6 11 28 12 0.0 6 12 29 7 0.0 6 7 30 10 0.0 6 9 1 31 3 0.0 6 3 32 3 0.0 6 3 33 7 0.0 6 6 1 34 5 0.0 6 5 35 6 0.0 6 5 1 36 3 0.0 6 3 37 4 0.0 6 4 38 5 0.0 6 3 2 39 9 0.0 6 9 40 9 0.0 6 9 41 7 0.0 6 7 42 7 0.0 6 6 0 1 43 9 0.0 6 7 2 44 8 0.0 6 8 45 4 0.0 6 3 0 0 1 46 11 0.0 6 11 47 11 0.0 6 9 2 48 12 0.0 6 11 1 49 17 0.0 6 15 2 50 4 0.0 6 4 51 10 0.0 6 9 1 52 10 0.0 6 9 1 53 6 0.0 6 2 4 54 6 0.0 6 6 55 11 0.0 6 11 56 25 0.0 6 22 3 57 24 0.0 6 22 2 58 8 0.0 6 7 0 1 59 11 0.0 6 8 3 60 15 0.0 6 15 61 9 0.0 6 7 2 62 12 0.0 6 11 1 63 11 0.0 6 8 2 0 0 1 64 11 0.0 6 9 2 65 7 0.0 6 7 66 17 0.0 6 16 1 67 10 0.0 6 8 1 1 68 24 0.0 6 21 3 69 27 0.0 6 24 2 1 70 114 0.0 6 109 4 1 71 22 0.0 6 19 2 0 0 1 72 69 0.0 6 66 3 73 18 0.0 6 17 0 0 1 74 26 0.0 6 24 1 1 75 18 0.0 6 16 1 1 76 15 0.0 6 15 77 14 0.0 6 14 78 19 0.0 6 18 1 79 16 0.0 6 16 80 12 0.0 6 11 1 81 9 0.0 6 8 0 1 82 28 0.0 6 27 0 0 0 1 83 36 0.0 6 31 3 2 84 31 0.0 6 29 2 85 40 0.0 6 38 2 86 37 0.0 6 34 3 87 34 0.0 6 33 1 88 18 0.0 6 18 89 16 0.0 6 14 2 90 41 0.0 6 39 1 1 91 21 0.0 6 20 1 92 33 0.0 6 32 1 93 30 0.0 6 29 0 0 1 94 53 0.0 6 51 2 95 28 0.0 6 25 2 0 0 1 96 42 0.0 6 41 1 97 29 0.0 6 29 98 26 0.0 6 25 0 1 99 66 0.0 6 61 4 1 100 25 0.0 6 23 2 101 33 0.0 6 29 4 102 44 0.0 6 43 1 103 66 0.0 6 62 3 0 0 1 104 52 0.0 6 50 0 1 0 1 105 48 0.0 6 43 4 1 106 39 0.0 6 38 1 107 79 0.0 6 78 1 108 36 0.0 6 35 1 109 43 0.0 6 42 0 1 110 38 0.0 6 38 111 29 0.0 6 27 2 112 50 0.0 6 49 0 0 0 1 113 39 0.0 6 37 2 114 38 0.0 6 35 3 115 55 0.0 6 52 3 116 35 0.0 6 33 2 117 34 0.0 6 32 2 118 56 0.0 6 56 119 49 0.0 6 48 1 120 83 0.0 6 81 2 121 54 0.0 6 53 1 122 79 0.0 6 75 3 1 123 81 0.0 6 80 1 124 102 0.0 6 92 9 0 0 1 125 72 0.0 6 68 4 126 60 0.0 6 57 3 127 60 0.0 6 59 1 128 75 0.0 6 72 3 129 69 0.0 6 67 2 130 94 0.0 6 93 1 131 113 0.0 6 109 4 132 160 0.0 6 154 5 0 0 1 133 124 0.0 6 122 1 0 0 1 134 181 0.0 6 173 8 135 268 0.0 6 256 12 136 206 0.0 6 204 1 1 137 282 0.0 6 275 6 0 0 0 1 138 462 0.0 6 455 7 139 244 0.0 6 241 3 140 251 0.0 6 246 5 141 362 0.0 6 355 7 142 290 0.0 6 285 5 143 385 0.0 6 372 12 0 0 1 144 439 0.0 6 432 7 145 278 0.0 6 271 5 1 0 1 146 266 0.0 6 262 2 1 0 1 147 255 0.0 6 253 2 148 467 0.0 6 458 9 149 425 0.0 6 413 11 1 150 417 0.0 6 408 9 151 424 0.0 6 417 6 1 152 469 0.0 6 454 13 1 0 1 153 563 0.0 6 552 10 1 154 423 0.0 6 412 11 155 537 0.0 6 524 13 156 549 0.0 6 539 9 1 157 553 0.0 6 536 14 0 2 1 158 823 0.0 6 810 12 1 159 425 0.0 6 409 14 2 160 664 0.0 6 653 11 161 604 0.0 6 590 13 0 0 0 0 1 162 444 0.0 6 438 5 1 163 553 0.0 6 544 8 0 0 0 0 1 164 650 0.0 6 634 15 1 165 670 0.0 6 654 14 1 1 166 798 0.0 6 785 13 167 440 0.0 6 430 10 168 518 0.0 6 508 10 169 252 0.0 6 244 7 1 170 278 0.0 6 271 7 171 479 0.0 6 459 19 0 0 1 172 276 0.0 6 272 4 173 361 0.0 6 350 10 1 174 442 0.0 6 429 13 175 994 0.0 6 967 25 1 0 1 176 493 0.0 6 467 26 177 599 0.0 6 581 17 0 1 178 409 0.0 6 402 6 1 179 530 0.0 6 511 16 0 2 1 180 539 0.0 6 516 21 1 0 1 181 691 0.0 6 667 23 1 182 379 0.0 6 365 14 183 595 0.0 6 570 22 0 2 0 0 1 184 216 0.0 6 210 5 0 0 1 185 215 0.0 6 206 9 186 774 0.0 6 744 28 2 187 985 0.0 6 954 31 188 1085 0.0 6 1048 35 1 1 189 948 0.0 6 916 31 0 0 1 190 1215 0.0 6 1170 43 1 0 1 191 1029 0.0 6 995 34 192 811 0.0 6 789 20 0 0 2 193 653 0.0 6 631 22 194 915 0.0 6 866 42 3 1 3 195 835 0.0 6 798 37 196 2146 0.0 6 2077 65 2 0 2 197 582 0.0 6 563 18 1 198 1225 0.0 6 1174 51 199 2509 0.0 6 2430 73 1 0 5 200 642 0.0 6 620 22 201 967 0.0 6 938 26 3 202 558 0.0 6 538 20 203 423 0.0 6 410 13 204 550 0.0 6 534 15 0 0 1 205 268 0.0 6 259 9 206 266 0.0 6 261 5 207 222 0.0 6 208 12 1 0 1 208 287 0.0 6 278 8 0 0 1 209 334 0.0 6 327 6 1 210 310 0.0 6 300 9 1 211 396 0.0 6 382 14 212 385 0.0 6 377 8 213 85 0.0 6 81 4 214 270 0.0 6 264 5 1 215 164 0.0 6 161 2 0 0 1 216 384 0.0 6 370 12 1 1 217 679 0.0 6 655 23 1 218 301 0.0 6 295 5 0 0 1 219 298 0.0 6 283 15 220 228 0.0 6 218 9 1 221 384 0.0 6 371 13 222 395 0.0 6 380 15 223 537 0.0 6 517 19 0 0 1 224 174 0.0 6 168 6 225 317 0.0 6 306 10 1 226 222 0.0 6 218 3 1 227 201 0.0 6 195 6 228 149 0.0 6 145 4 229 197 0.0 6 191 6 230 119 0.0 6 117 2 231 175 0.0 6 170 4 0 0 1 232 192 0.0 6 184 8 233 176 0.0 6 169 7 234 94 0.0 6 92 2 235 63 0.0 6 61 2 236 30 0.0 6 29 1 237 45 0.0 6 43 2 238 28 0.0 6 28 239 32 0.0 6 31 1 240 39 0.0 6 38 1 241 78 0.0 6 75 3 242 133 0.0 6 127 5 1 243 93 0.0 6 88 5 244 144 0.0 6 141 3 245 51 0.0 6 48 3 246 36 0.0 6 36 247 13 0.0 6 12 1 248 31 0.0 6 31 249 18 0.0 6 17 1 250 16 0.0 6 16 251 35 0.0 6 34 1 252 5 0.0 6 5 253 10 0.0 6 9 1 254 18 0.0 6 18 255 5 0.0 6 5 256 7 0.0 6 7 257 3 0.0 6 3 261 1 0.0 6 1 262 1 0.0 6 1 266 1 0.0 6 1 280 3 0.0 6 3 281 16 0.0 6 16 282 21 0.0 6 20 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 527 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 68 2 0.0 6 2 69 17 0.0 6 17 70 4 0.0 6 4 71 4 0.0 6 4 72 6 0.0 6 6 74 10 0.0 6 10 75 1 0.0 6 1 76 1 0.0 6 1 77 3 0.0 6 3 78 4 0.0 6 4 79 14 0.0 6 14 80 5 0.0 6 5 82 9 0.0 6 9 83 1 0.0 6 1 84 7 0.0 6 7 85 1 0.0 6 1 88 4 0.0 6 4 89 17 0.0 6 17 90 3 0.0 6 3 91 9 0.0 6 9 92 9 0.0 6 9 93 3 0.0 6 1 1 0 0 1 94 9 0.0 6 9 95 20 0.0 6 20 96 24 0.0 6 23 1 97 28 0.0 6 28 98 28 0.0 6 28 105 2 0.0 6 2 106 33 0.0 6 33 110 1 0.0 6 1 111 10 0.0 6 10 112 1 0.0 6 1 113 1 0.0 6 1 114 1 0.0 6 1 117 9 0.0 6 9 118 1 0.0 6 1 119 2 0.0 6 2 120 6 0.0 6 6 122 3 0.0 6 3 123 15 0.0 6 15 127 1 0.0 6 0 0 0 0 1 128 1 0.0 6 1 129 10 0.0 6 10 130 59 0.0 6 58 1 131 2 0.0 6 2 132 1 0.0 6 0 0 1 136 1 0.0 6 1 140 12 0.0 6 12 141 37 0.0 6 37 142 4 0.0 6 4 145 1 0.0 6 1 146 11 0.0 6 11 156 3 0.0 6 3 157 2 0.0 6 2 161 4 0.0 6 4 162 12 0.0 6 12 190 1 0.0 6 1 196 1 0.0 6 1 198 7 0.0 6 7 199 29 0.0 6 29 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_10_167_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_10_394_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_10_167_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_10_394_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.78 s (42 µs/read; 1.42 M reads/minute). === Summary === Total read pairs processed: 42,184 Read 1 with adapter: 42,183 (100.0%) Read 2 with adapter: 42,158 (99.9%) == Read fate breakdown == Pairs that were too short: 1 (0.0%) Pairs discarded as untrimmed: 27 (0.1%) Pairs written (passing filters): 42,156 (99.9%) Total basepairs processed: 20,783,981 bp Read 1: 11,878,380 bp Read 2: 8,905,601 bp Total written (filtered): 2,619,896 bp (12.6%) Read 1: 1,268,233 bp Read 2: 1,351,663 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 42113 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 659.1 0 1 1 6 1 10.3 1 0 0 1 15 1 0.0 4 1 19 1 0.0 5 1 20 5 0.0 6 5 21 3 0.0 6 2 1 22 4 0.0 6 4 23 1 0.0 6 1 24 1 0.0 6 1 25 6 0.0 6 5 1 26 6 0.0 6 5 1 27 2 0.0 6 2 28 4 0.0 6 4 29 2 0.0 6 2 30 1 0.0 6 1 32 1 0.0 6 0 1 34 2 0.0 6 2 35 1 0.0 6 1 36 1 0.0 6 1 37 3 0.0 6 2 1 39 1 0.0 6 1 40 4 0.0 6 3 0 1 41 2 0.0 6 1 1 42 1 0.0 6 1 44 5 0.0 6 5 45 3 0.0 6 3 50 4 0.0 6 3 0 0 0 1 52 1 0.0 6 0 1 53 1 0.0 6 1 54 2 0.0 6 2 56 2 0.0 6 2 57 2 0.0 6 1 0 1 58 1 0.0 6 1 59 1 0.0 6 0 1 60 4 0.0 6 3 1 61 4 0.0 6 3 1 62 1 0.0 6 1 63 1 0.0 6 0 0 1 64 3 0.0 6 3 67 2 0.0 6 2 68 1 0.0 6 1 69 1 0.0 6 1 70 1 0.0 6 1 72 1 0.0 6 1 73 2 0.0 6 2 74 1 0.0 6 1 75 1 0.0 6 1 77 2 0.0 6 2 78 2 0.0 6 2 79 1 0.0 6 1 80 2 0.0 6 2 81 3 0.0 6 3 82 2 0.0 6 2 83 1 0.0 6 1 84 6 0.0 6 6 86 7 0.0 6 7 87 1 0.0 6 1 88 1 0.0 6 1 89 1 0.0 6 1 90 1 0.0 6 1 91 4 0.0 6 4 92 3 0.0 6 3 93 2 0.0 6 2 94 3 0.0 6 3 95 2 0.0 6 2 96 1 0.0 6 1 97 2 0.0 6 2 98 3 0.0 6 3 99 1 0.0 6 1 100 1 0.0 6 1 101 6 0.0 6 6 102 8 0.0 6 7 1 103 1 0.0 6 1 104 1 0.0 6 1 105 7 0.0 6 6 1 106 2 0.0 6 2 107 7 0.0 6 7 108 7 0.0 6 7 109 2 0.0 6 2 110 3 0.0 6 3 111 6 0.0 6 6 112 5 0.0 6 4 1 113 3 0.0 6 3 114 6 0.0 6 6 115 5 0.0 6 5 116 5 0.0 6 4 1 117 8 0.0 6 8 118 9 0.0 6 9 119 3 0.0 6 3 120 3 0.0 6 2 1 121 4 0.0 6 3 0 0 0 0 1 122 10 0.0 6 10 123 9 0.0 6 8 1 124 6 0.0 6 5 1 125 8 0.0 6 6 2 126 4 0.0 6 3 0 0 1 127 5 0.0 6 5 128 14 0.0 6 12 2 129 8 0.0 6 8 130 7 0.0 6 7 131 5 0.0 6 5 132 13 0.0 6 13 133 6 0.0 6 6 134 5 0.0 6 5 135 8 0.0 6 8 136 10 0.0 6 10 137 23 0.0 6 22 1 138 16 0.0 6 16 139 8 0.0 6 8 140 11 0.0 6 10 1 141 19 0.0 6 19 142 27 0.0 6 26 1 143 39 0.0 6 39 144 43 0.0 6 42 1 145 47 0.0 6 47 146 39 0.0 6 38 1 147 50 0.0 6 49 1 148 81 0.0 6 80 1 149 87 0.0 6 86 1 150 74 0.0 6 73 1 151 61 0.0 6 61 152 84 0.0 6 84 153 103 0.0 6 103 154 65 0.0 6 64 1 155 55 0.0 6 55 156 67 0.0 6 65 2 157 47 0.0 6 46 1 158 66 0.0 6 65 1 159 46 0.0 6 46 160 51 0.0 6 51 161 39 0.0 6 39 162 58 0.0 6 58 163 70 0.0 6 70 164 63 0.0 6 60 3 165 44 0.0 6 44 166 54 0.0 6 53 1 167 57 0.0 6 57 168 49 0.0 6 49 169 68 0.0 6 67 1 170 55 0.0 6 53 2 171 46 0.0 6 45 1 172 59 0.0 6 59 173 69 0.0 6 68 1 174 55 0.0 6 54 1 175 59 0.0 6 58 1 176 48 0.0 6 47 1 177 57 0.0 6 55 2 178 62 0.0 6 61 1 179 59 0.0 6 56 3 180 63 0.0 6 60 3 181 113 0.0 6 111 2 182 69 0.0 6 65 4 183 58 0.0 6 56 2 184 86 0.0 6 85 1 185 87 0.0 6 85 2 186 98 0.0 6 97 1 187 67 0.0 6 64 3 188 106 0.0 6 104 2 189 64 0.0 6 63 1 190 95 0.0 6 91 3 1 191 140 0.0 6 137 3 192 103 0.0 6 101 1 1 193 121 0.0 6 121 194 119 0.0 6 113 5 1 195 133 0.0 6 131 2 196 144 0.0 6 140 4 197 152 0.0 6 145 7 198 187 0.0 6 184 3 199 171 0.0 6 169 2 200 222 0.0 6 215 7 201 165 0.0 6 160 5 202 205 0.0 6 195 10 203 185 0.0 6 181 2 2 204 191 0.0 6 187 4 205 222 0.0 6 217 5 206 164 0.0 6 157 7 207 203 0.0 6 197 5 1 208 215 0.0 6 206 9 209 166 0.0 6 162 4 210 140 0.0 6 136 4 211 113 0.0 6 108 5 212 111 0.0 6 108 3 213 147 0.0 6 144 3 214 223 0.0 6 213 10 215 142 0.0 6 139 3 216 194 0.0 6 189 5 217 238 0.0 6 235 3 218 178 0.0 6 171 7 219 176 0.0 6 174 2 220 153 0.0 6 152 1 221 121 0.0 6 120 0 1 222 168 0.0 6 166 2 223 232 0.0 6 224 8 224 197 0.0 6 190 6 1 225 183 0.0 6 177 6 226 270 0.0 6 265 5 227 274 0.0 6 270 4 228 225 0.0 6 217 7 1 229 203 0.0 6 201 2 230 211 0.0 6 208 3 231 130 0.0 6 124 6 232 186 0.0 6 184 1 0 0 1 233 153 0.0 6 150 3 234 196 0.0 6 191 5 235 166 0.0 6 163 3 236 185 0.0 6 174 10 1 237 178 0.0 6 172 6 238 256 0.0 6 248 7 0 1 239 232 0.0 6 230 2 240 282 0.0 6 277 5 241 324 0.0 6 312 11 1 242 331 0.0 6 322 8 1 243 194 0.0 6 189 5 244 193 0.0 6 183 9 1 245 213 0.0 6 208 5 246 207 0.0 6 199 7 0 0 1 247 183 0.0 6 176 7 248 197 0.0 6 189 7 1 249 229 0.0 6 221 7 1 250 226 0.0 6 221 5 251 264 0.0 6 258 6 252 225 0.0 6 219 6 253 192 0.0 6 185 6 1 254 247 0.0 6 238 9 255 230 0.0 6 220 10 256 300 0.0 6 295 5 257 302 0.0 6 294 7 0 0 1 258 193 0.0 6 185 7 1 259 442 0.0 6 426 16 260 477 0.0 6 466 11 261 246 0.0 6 236 9 1 262 251 0.0 6 240 11 263 265 0.0 6 261 4 264 211 0.0 6 203 8 265 245 0.0 6 239 6 266 184 0.0 6 180 4 267 184 0.0 6 178 5 1 268 239 0.0 6 228 11 269 359 0.0 6 351 7 1 270 184 0.0 6 176 8 271 372 0.0 6 352 19 0 0 1 272 349 0.0 6 336 11 2 273 325 0.0 6 315 9 0 0 1 274 407 0.0 6 393 12 2 275 348 0.0 6 335 12 1 276 519 0.0 6 504 14 1 277 822 0.0 6 803 18 0 1 278 738 0.0 6 712 26 279 2117 0.0 6 2080 35 0 2 280 3801 0.0 6 3716 78 6 1 281 12138 0.0 6 11943 188 5 1 1 282 7 0.0 6 5 1 1 283 2 0.0 6 2 284 1 0.0 6 1 286 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 70 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 7 0.0 6 7 70 1 0.0 6 1 74 1 0.0 6 1 75 1 0.0 6 1 77 5 0.0 6 5 78 1 0.0 6 1 79 1 0.0 6 1 80 1 0.0 6 1 81 1 0.0 6 1 84 1 0.0 6 1 85 3 0.0 6 3 86 2 0.0 6 2 87 1 0.0 6 1 88 1 0.0 6 1 89 1 0.0 6 1 90 1 0.0 6 1 91 1 0.0 6 1 92 1 0.0 6 1 98 1 0.0 6 1 102 2 0.0 6 2 104 1 0.0 6 1 107 1 0.0 6 1 109 1 0.0 6 1 110 1 0.0 6 1 111 1 0.0 6 1 118 1 0.0 6 1 125 1 0.0 6 1 128 1 0.0 6 1 130 1 0.0 6 1 131 1 0.0 6 1 137 1 0.0 6 1 138 1 0.0 6 1 141 2 0.0 6 2 142 1 0.0 6 1 143 1 0.0 6 1 148 1 0.0 6 1 149 1 0.0 6 1 150 1 0.0 6 1 155 1 0.0 6 1 157 1 0.0 6 0 1 160 1 0.0 6 1 161 1 0.0 6 1 168 1 0.0 6 1 179 1 0.0 6 1 188 1 0.0 6 1 192 1 0.0 6 1 200 1 0.0 6 1 202 1 0.0 6 1 205 1 0.0 6 1 226 1 0.0 6 1 234 1 0.0 6 1 235 1 0.0 6 1 242 1 0.0 6 1 259 1 0.0 6 1 261 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 42080 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 10.3 1 1 7 1 2.6 2 1 8 1 0.6 2 0 1 9 1 0.2 2 1 10 7 0.0 3 7 11 2 0.0 3 2 12 1 0.0 3 1 13 6 0.0 3 5 1 14 7 0.0 4 6 0 0 0 1 15 1 0.0 4 1 16 7 0.0 4 6 1 17 10 0.0 5 9 0 1 18 4 0.0 5 2 2 19 7 0.0 5 3 4 20 45 0.0 6 42 3 21 29 0.0 6 29 22 17 0.0 6 17 23 22 0.0 6 22 24 16 0.0 6 15 1 25 17 0.0 6 16 1 26 3 0.0 6 3 27 6 0.0 6 6 28 8 0.0 6 7 1 29 4 0.0 6 4 30 17 0.0 6 16 1 31 8 0.0 6 7 1 32 5 0.0 6 5 33 7 0.0 6 6 0 0 1 34 8 0.0 6 7 1 35 2 0.0 6 2 36 4 0.0 6 4 37 7 0.0 6 5 2 38 8 0.0 6 7 1 39 4 0.0 6 4 40 2 0.0 6 2 41 10 0.0 6 10 42 3 0.0 6 3 43 8 0.0 6 6 2 44 16 0.0 6 15 1 45 8 0.0 6 8 46 18 0.0 6 17 0 1 47 16 0.0 6 13 2 1 48 5 0.0 6 4 0 1 49 9 0.0 6 9 50 14 0.0 6 12 1 0 1 51 8 0.0 6 7 1 52 18 0.0 6 15 3 53 11 0.0 6 8 3 54 20 0.0 6 15 3 1 0 0 1 55 17 0.0 6 16 1 56 13 0.0 6 13 57 16 0.0 6 14 2 58 14 0.0 6 12 2 59 4 0.0 6 4 60 8 0.0 6 7 1 61 8 0.0 6 7 0 1 62 9 0.0 6 8 0 0 0 1 63 10 0.0 6 10 64 13 0.0 6 12 1 65 5 0.0 6 3 1 0 1 66 23 0.0 6 19 4 67 82 0.0 6 81 1 68 25 0.0 6 21 3 0 0 1 69 20 0.0 6 19 1 70 13 0.0 6 11 1 0 0 0 1 71 22 0.0 6 22 72 16 0.0 6 16 73 10 0.0 6 8 2 74 10 0.0 6 10 75 15 0.0 6 13 2 76 14 0.0 6 13 0 1 77 14 0.0 6 13 1 78 10 0.0 6 9 1 79 14 0.0 6 13 0 1 80 18 0.0 6 16 1 1 81 11 0.0 6 8 2 0 0 1 82 21 0.0 6 18 3 83 38 0.0 6 36 2 84 34 0.0 6 29 4 1 85 19 0.0 6 18 1 86 14 0.0 6 13 0 0 0 0 0 1 87 28 0.0 6 27 1 88 12 0.0 6 11 1 89 25 0.0 6 22 3 90 30 0.0 6 27 3 91 22 0.0 6 21 1 92 26 0.0 6 21 4 1 93 39 0.0 6 37 2 94 54 0.0 6 47 6 1 95 30 0.0 6 29 0 0 0 1 96 36 0.0 6 34 2 97 20 0.0 6 20 98 27 0.0 6 25 1 1 99 33 0.0 6 31 2 100 29 0.0 6 27 2 101 39 0.0 6 34 5 102 46 0.0 6 43 3 103 66 0.0 6 64 2 104 54 0.0 6 52 2 105 53 0.0 6 52 1 106 34 0.0 6 30 4 107 36 0.0 6 34 2 108 41 0.0 6 38 3 109 54 0.0 6 51 3 110 45 0.0 6 42 3 111 46 0.0 6 45 1 112 37 0.0 6 35 2 113 39 0.0 6 37 1 1 114 63 0.0 6 56 7 115 39 0.0 6 39 116 40 0.0 6 37 3 117 48 0.0 6 47 1 118 45 0.0 6 43 2 119 59 0.0 6 54 5 120 67 0.0 6 60 7 121 55 0.0 6 52 3 122 81 0.0 6 80 1 123 43 0.0 6 42 1 124 83 0.0 6 79 3 1 125 82 0.0 6 79 3 126 64 0.0 6 59 4 0 0 1 127 82 0.0 6 81 1 128 59 0.0 6 58 1 129 63 0.0 6 62 0 1 130 69 0.0 6 68 1 131 121 0.0 6 120 1 132 130 0.0 6 124 6 133 116 0.0 6 112 4 134 157 0.0 6 150 7 135 234 0.0 6 225 9 136 138 0.0 6 134 4 137 143 0.0 6 135 8 138 93 0.0 6 89 3 1 139 182 0.0 6 175 6 0 0 1 140 202 0.0 6 193 7 1 0 1 141 194 0.0 6 186 8 142 273 0.0 6 266 7 143 265 0.0 6 256 8 1 144 343 0.0 6 334 8 0 0 1 145 213 0.0 6 204 9 146 223 0.0 6 217 6 147 171 0.0 6 169 1 0 1 148 260 0.0 6 251 8 1 149 292 0.0 6 283 7 1 0 1 150 515 0.0 6 496 18 0 0 1 151 213 0.0 6 201 12 152 520 0.0 6 508 10 0 2 153 307 0.0 6 296 9 0 0 2 154 319 0.0 6 309 8 1 0 1 155 292 0.0 6 284 8 156 239 0.0 6 235 3 0 1 157 360 0.0 6 349 10 1 158 384 0.0 6 376 8 159 206 0.0 6 201 5 160 316 0.0 6 311 5 161 198 0.0 6 193 5 162 141 0.0 6 137 3 0 1 163 275 0.0 6 274 1 164 265 0.0 6 259 6 165 327 0.0 6 319 8 166 390 0.0 6 381 8 1 167 231 0.0 6 224 7 168 458 0.0 6 452 5 1 169 177 0.0 6 170 7 170 218 0.0 6 210 8 171 474 0.0 6 466 7 1 172 237 0.0 6 232 5 173 330 0.0 6 315 13 0 1 1 174 487 0.0 6 473 13 1 175 717 0.0 6 703 12 1 0 1 176 471 0.0 6 457 12 2 177 415 0.0 6 401 13 0 0 1 178 354 0.0 6 349 5 179 417 0.0 6 407 7 1 0 2 180 343 0.0 6 330 13 181 493 0.0 6 479 13 0 0 0 0 1 182 126 0.0 6 119 7 183 460 0.0 6 453 6 0 0 1 184 401 0.0 6 386 13 1 1 185 549 0.0 6 534 13 0 1 1 186 849 0.0 6 828 20 1 187 786 0.0 6 768 16 1 1 188 782 0.0 6 761 21 189 737 0.0 6 710 23 3 1 190 1052 0.0 6 1024 26 1 0 1 191 976 0.0 6 946 29 0 0 1 192 561 0.0 6 547 13 1 193 407 0.0 6 393 13 1 194 681 0.0 6 666 13 2 195 974 0.0 6 941 30 2 0 1 196 1716 0.0 6 1665 46 3 0 2 197 570 0.0 6 546 20 2 1 0 0 1 198 1096 0.0 6 1071 25 199 1500 0.0 6 1463 32 4 0 0 1 200 560 0.0 6 533 26 0 1 201 774 0.0 6 752 20 0 1 1 202 436 0.0 6 420 15 1 203 367 0.0 6 363 4 204 585 0.0 6 568 16 1 205 198 0.0 6 191 7 206 202 0.0 6 199 3 207 125 0.0 6 121 4 208 242 0.0 6 236 6 209 197 0.0 6 192 5 210 180 0.0 6 175 4 0 1 211 171 0.0 6 167 4 212 258 0.0 6 254 4 213 113 0.0 6 110 3 214 386 0.0 6 378 8 215 349 0.0 6 337 12 216 345 0.0 6 337 6 1 0 1 217 299 0.0 6 288 10 1 218 229 0.0 6 225 4 219 272 0.0 6 265 6 0 1 220 93 0.0 6 93 221 268 0.0 6 262 6 222 262 0.0 6 253 9 223 417 0.0 6 410 7 224 182 0.0 6 177 5 225 253 0.0 6 246 6 1 226 133 0.0 6 128 5 227 190 0.0 6 181 9 228 108 0.0 6 104 4 229 214 0.0 6 202 11 1 230 173 0.0 6 168 4 1 231 180 0.0 6 175 5 232 256 0.0 6 249 7 233 107 0.0 6 102 5 234 71 0.0 6 68 3 235 32 0.0 6 31 1 236 24 0.0 6 23 1 237 16 0.0 6 15 1 238 41 0.0 6 40 1 239 20 0.0 6 19 1 240 46 0.0 6 46 241 96 0.0 6 94 2 242 119 0.0 6 116 3 243 81 0.0 6 79 2 244 56 0.0 6 54 2 245 42 0.0 6 40 2 246 51 0.0 6 51 247 20 0.0 6 18 2 248 51 0.0 6 48 3 249 3 0.0 6 3 250 6 0.0 6 6 251 23 0.0 6 22 1 252 1 0.0 6 1 253 2 0.0 6 2 254 1 0.0 6 1 260 1 0.0 6 1 278 11 0.0 6 11 279 18 0.0 6 18 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 78 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 75 2 0.0 6 2 76 2 0.0 6 2 77 1 0.0 6 1 78 1 0.0 6 1 79 2 0.0 6 2 80 3 0.0 6 3 82 2 0.0 6 2 83 2 0.0 6 2 84 1 0.0 6 1 85 2 0.0 6 2 86 1 0.0 6 1 87 3 0.0 6 3 89 1 0.0 6 1 90 1 0.0 6 1 91 2 0.0 6 2 92 3 0.0 6 3 94 1 0.0 6 1 95 2 0.0 6 2 96 1 0.0 6 1 98 2 0.0 6 2 100 1 0.0 6 1 101 3 0.0 6 3 102 2 0.0 6 2 110 1 0.0 6 1 111 1 0.0 6 1 113 2 0.0 6 2 114 1 0.0 6 1 117 1 0.0 6 1 120 1 0.0 6 1 124 1 0.0 6 1 128 1 0.0 6 1 129 2 0.0 6 2 133 1 0.0 6 1 136 1 0.0 6 1 137 1 0.0 6 1 138 1 0.0 6 1 140 1 0.0 6 1 143 1 0.0 6 1 144 1 0.0 6 1 145 2 0.0 6 2 149 1 0.0 6 1 152 2 0.0 6 2 156 1 0.0 6 1 158 1 0.0 6 1 160 1 0.0 6 1 161 2 0.0 6 2 165 1 0.0 6 1 167 1 0.0 6 1 169 1 0.0 6 1 175 1 0.0 6 1 180 1 0.0 6 1 182 1 0.0 6 1 183 1 0.0 6 1 201 1 0.0 6 1 204 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_12_156_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_12_383_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_12_156_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_12_383_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_13_157_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_14-Rev_13_384_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_13_157_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_14-Rev_13_384_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_08_158_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_08_385_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_08_158_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_08_385_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_09_159_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_09_386_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_09_159_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_09_386_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_10_160_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_10_387_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_10_160_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_10_387_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_11_161_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_11_388_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_11_161_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_11_388_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_12_162_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_12_389_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_12_162_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_12_389_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_13_163_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_15-Rev_13_390_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_13_163_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_15-Rev_13_390_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_07_164_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_07_391_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_07_164_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_07_391_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_08_165_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_08_392_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_08_165_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_08_392_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_09_166_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_09_393_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_09_166_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_09_393_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_10_167_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_10_394_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_10_167_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_10_394_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_11_168_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_11_395_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_11_168_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_11_395_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.65 s (42 µs/read; 1.44 M reads/minute). === Summary === Total read pairs processed: 63,323 Read 1 with adapter: 63,313 (100.0%) Read 2 with adapter: 63,301 (100.0%) == Read fate breakdown == Pairs that were too short: 43 (0.1%) Pairs discarded as untrimmed: 32 (0.1%) Pairs written (passing filters): 63,248 (99.9%) Total basepairs processed: 26,836,710 bp Read 1: 15,012,541 bp Read 2: 11,824,169 bp Total written (filtered): 3,848,259 bp (14.3%) Read 1: 1,975,042 bp Read 2: 1,873,217 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 61695 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 989.4 0 0 1 4 1 247.4 1 0 1 6 1 15.5 1 0 0 1 9 1 0.2 2 1 10 1 0.1 3 1 12 1 0.0 3 1 15 1 0.0 4 1 16 2 0.0 4 2 17 1 0.0 5 0 1 19 3 0.0 5 3 20 103 0.0 6 101 2 21 56 0.0 6 55 1 22 49 0.0 6 47 1 1 23 20 0.0 6 16 3 1 24 21 0.0 6 21 25 9 0.0 6 8 1 26 22 0.0 6 21 1 27 14 0.0 6 14 28 7 0.0 6 7 29 9 0.0 6 8 1 30 3 0.0 6 2 1 31 8 0.0 6 8 32 7 0.0 6 6 1 33 10 0.0 6 10 34 9 0.0 6 9 35 3 0.0 6 3 36 5 0.0 6 5 37 5 0.0 6 5 38 6 0.0 6 6 39 8 0.0 6 8 40 4 0.0 6 4 41 6 0.0 6 6 42 2 0.0 6 2 43 6 0.0 6 6 44 9 0.0 6 7 2 45 4 0.0 6 4 46 5 0.0 6 4 1 47 3 0.0 6 3 48 9 0.0 6 9 49 7 0.0 6 6 1 50 6 0.0 6 6 51 8 0.0 6 8 52 5 0.0 6 5 53 5 0.0 6 5 54 6 0.0 6 6 55 2 0.0 6 2 56 4 0.0 6 4 57 4 0.0 6 4 58 2 0.0 6 2 59 5 0.0 6 5 60 5 0.0 6 4 1 61 4 0.0 6 4 62 2 0.0 6 2 63 5 0.0 6 5 64 6 0.0 6 6 65 7 0.0 6 7 66 6 0.0 6 6 67 5 0.0 6 5 68 5 0.0 6 5 69 4 0.0 6 3 1 70 6 0.0 6 5 1 71 4 0.0 6 4 72 6 0.0 6 6 73 8 0.0 6 8 74 6 0.0 6 6 75 7 0.0 6 7 76 7 0.0 6 7 77 17 0.0 6 17 78 5 0.0 6 5 79 9 0.0 6 9 80 7 0.0 6 7 81 7 0.0 6 6 1 82 7 0.0 6 6 0 1 83 6 0.0 6 5 1 84 7 0.0 6 7 85 13 0.0 6 13 86 15 0.0 6 15 87 14 0.0 6 14 88 11 0.0 6 10 1 89 9 0.0 6 9 90 7 0.0 6 7 91 12 0.0 6 12 92 9 0.0 6 8 1 93 8 0.0 6 8 94 9 0.0 6 8 1 95 6 0.0 6 6 96 12 0.0 6 9 3 97 8 0.0 6 8 98 4 0.0 6 4 99 7 0.0 6 6 1 100 16 0.0 6 15 0 0 0 1 101 14 0.0 6 13 1 102 18 0.0 6 18 103 6 0.0 6 6 104 15 0.0 6 14 1 105 13 0.0 6 13 106 25 0.0 6 25 107 20 0.0 6 20 108 24 0.0 6 23 1 109 19 0.0 6 19 110 11 0.0 6 11 111 12 0.0 6 12 112 25 0.0 6 25 113 22 0.0 6 22 114 29 0.0 6 29 115 13 0.0 6 12 0 0 0 0 0 1 116 17 0.0 6 17 117 23 0.0 6 23 118 20 0.0 6 20 119 21 0.0 6 20 1 120 22 0.0 6 22 121 15 0.0 6 14 1 122 18 0.0 6 17 1 123 22 0.0 6 21 1 124 25 0.0 6 24 1 125 18 0.0 6 18 126 27 0.0 6 27 127 17 0.0 6 17 128 23 0.0 6 23 129 28 0.0 6 28 130 37 0.0 6 37 131 27 0.0 6 27 132 25 0.0 6 25 133 40 0.0 6 39 1 134 34 0.0 6 33 0 1 135 27 0.0 6 27 136 35 0.0 6 35 137 36 0.0 6 36 138 51 0.0 6 51 139 41 0.0 6 41 140 144 0.0 6 143 1 141 336 0.0 6 331 5 142 468 0.0 6 465 3 143 314 0.0 6 314 144 316 0.0 6 315 1 145 466 0.0 6 464 2 146 429 0.0 6 428 1 147 446 0.0 6 444 2 148 376 0.0 6 376 149 426 0.0 6 425 1 150 373 0.0 6 372 1 151 369 0.0 6 369 152 315 0.0 6 313 2 153 238 0.0 6 238 154 246 0.0 6 246 155 236 0.0 6 234 1 0 1 156 267 0.0 6 265 2 157 296 0.0 6 296 158 350 0.0 6 350 159 302 0.0 6 301 0 0 0 0 1 160 292 0.0 6 292 161 307 0.0 6 305 2 162 365 0.0 6 365 163 315 0.0 6 312 2 0 0 0 0 1 164 272 0.0 6 272 165 267 0.0 6 266 1 166 261 0.0 6 260 1 167 247 0.0 6 245 2 168 266 0.0 6 266 169 337 0.0 6 335 1 0 0 1 170 247 0.0 6 247 171 247 0.0 6 245 2 172 406 0.0 6 405 0 0 1 173 310 0.0 6 309 1 174 373 0.0 6 372 1 175 298 0.0 6 297 1 176 340 0.0 6 337 2 1 177 415 0.0 6 412 3 178 883 0.0 6 881 1 1 179 385 0.0 6 384 1 180 348 0.0 6 347 1 181 320 0.0 6 318 2 182 264 0.0 6 258 4 0 0 1 1 183 326 0.0 6 325 1 184 310 0.0 6 309 1 185 325 0.0 6 324 1 186 309 0.0 6 308 1 187 297 0.0 6 297 188 300 0.0 6 296 4 189 318 0.0 6 316 2 190 325 0.0 6 324 1 191 329 0.0 6 327 2 192 382 0.0 6 381 1 193 495 0.0 6 490 4 1 194 408 0.0 6 402 6 195 443 0.0 6 442 0 0 0 1 196 518 0.0 6 511 7 197 532 0.0 6 530 2 198 722 0.0 6 717 4 1 199 734 0.0 6 729 5 200 716 0.0 6 714 1 0 0 0 1 201 652 0.0 6 647 5 202 732 0.0 6 731 1 203 548 0.0 6 546 1 0 0 1 204 514 0.0 6 509 4 0 1 205 662 0.0 6 655 6 0 1 206 519 0.0 6 515 3 1 207 702 0.0 6 698 2 1 0 0 0 1 208 846 0.0 6 838 6 0 0 1 1 209 768 0.0 6 761 6 0 0 1 210 738 0.0 6 734 4 211 491 0.0 6 483 7 0 0 1 212 547 0.0 6 541 2 3 0 0 1 213 752 0.0 6 745 6 0 1 214 713 0.0 6 709 3 0 1 215 711 0.0 6 705 6 216 786 0.0 6 780 5 0 1 217 984 0.0 6 982 2 218 743 0.0 6 736 5 1 1 219 772 0.0 6 769 2 1 220 792 0.0 6 786 6 221 697 0.0 6 694 3 222 546 0.0 6 544 2 223 601 0.0 6 593 8 224 622 0.0 6 620 1 1 225 648 0.0 6 641 5 0 0 1 1 226 680 0.0 6 675 5 227 733 0.0 6 730 2 0 0 0 1 228 741 0.0 6 738 3 229 612 0.0 6 611 1 230 716 0.0 6 714 2 231 575 0.0 6 573 2 232 595 0.0 6 593 1 0 0 0 0 1 233 588 0.0 6 586 1 0 0 0 1 234 574 0.0 6 572 2 235 513 0.0 6 511 2 236 487 0.0 6 484 2 0 0 0 1 237 495 0.0 6 494 0 0 0 0 1 238 549 0.0 6 546 3 239 411 0.0 6 409 1 0 1 240 483 0.0 6 480 3 241 445 0.0 6 442 3 242 397 0.0 6 397 243 402 0.0 6 400 2 244 427 0.0 6 425 2 245 348 0.0 6 341 5 0 1 1 246 345 0.0 6 343 2 247 306 0.0 6 306 248 260 0.0 6 259 1 249 262 0.0 6 261 1 250 303 0.0 6 303 251 332 0.0 6 330 2 252 273 0.0 6 272 0 0 0 0 1 253 252 0.0 6 250 2 254 229 0.0 6 228 1 255 268 0.0 6 267 1 256 247 0.0 6 246 1 257 221 0.0 6 220 1 258 163 0.0 6 163 259 226 0.0 6 225 1 260 192 0.0 6 187 4 0 0 0 0 1 261 204 0.0 6 203 1 262 208 0.0 6 207 1 263 172 0.0 6 172 264 133 0.0 6 133 265 129 0.0 6 129 266 126 0.0 6 126 267 134 0.0 6 134 268 130 0.0 6 130 269 107 0.0 6 107 270 105 0.0 6 105 271 175 0.0 6 173 2 272 106 0.0 6 104 2 273 109 0.0 6 109 274 107 0.0 6 107 275 107 0.0 6 105 2 276 175 0.0 6 172 3 277 209 0.0 6 207 2 278 232 0.0 6 231 1 279 317 0.0 6 316 0 0 1 280 879 0.0 6 872 7 281 2099 0.0 6 2074 23 2 282 6 0.0 6 6 283 1 0.0 6 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1618 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 50 1 0.0 6 1 53 1 0.0 6 1 61 37 0.0 6 37 63 11 0.0 6 11 70 8 0.0 6 8 71 12 0.0 6 12 72 11 0.0 6 11 73 7 0.0 6 7 74 9 0.0 6 9 75 15 0.0 6 15 76 9 0.0 6 9 77 12 0.0 6 12 78 13 0.0 6 13 79 16 0.0 6 16 80 12 0.0 6 12 81 9 0.0 6 8 1 82 4 0.0 6 4 83 4 0.0 6 4 84 6 0.0 6 6 85 7 0.0 6 7 86 7 0.0 6 7 87 14 0.0 6 14 88 11 0.0 6 11 89 9 0.0 6 9 90 5 0.0 6 5 91 10 0.0 6 10 92 8 0.0 6 8 93 13 0.0 6 13 94 5 0.0 6 5 95 7 0.0 6 7 96 4 0.0 6 4 97 2 0.0 6 2 98 4 0.0 6 4 99 6 0.0 6 6 100 11 0.0 6 11 101 6 0.0 6 6 102 9 0.0 6 9 103 3 0.0 6 3 104 6 0.0 6 6 105 5 0.0 6 5 106 8 0.0 6 8 107 17 0.0 6 17 108 29 0.0 6 29 109 15 0.0 6 15 110 5 0.0 6 5 111 8 0.0 6 8 112 5 0.0 6 5 113 3 0.0 6 3 114 3 0.0 6 3 115 8 0.0 6 8 116 3 0.0 6 3 117 2 0.0 6 2 118 4 0.0 6 4 119 4 0.0 6 4 120 5 0.0 6 5 121 5 0.0 6 5 122 13 0.0 6 13 123 5 0.0 6 5 124 7 0.0 6 7 125 6 0.0 6 6 126 6 0.0 6 6 127 5 0.0 6 5 128 11 0.0 6 11 129 6 0.0 6 6 130 8 0.0 6 8 131 6 0.0 6 6 132 16 0.0 6 16 133 7 0.0 6 7 134 12 0.0 6 12 135 11 0.0 6 11 136 11 0.0 6 11 137 15 0.0 6 15 138 16 0.0 6 16 139 8 0.0 6 8 140 10 0.0 6 10 141 10 0.0 6 10 142 9 0.0 6 9 143 4 0.0 6 4 144 11 0.0 6 11 145 9 0.0 6 9 146 9 0.0 6 9 147 7 0.0 6 7 148 9 0.0 6 9 149 10 0.0 6 10 150 7 0.0 6 7 151 7 0.0 6 7 152 9 0.0 6 9 153 7 0.0 6 7 154 11 0.0 6 11 155 9 0.0 6 9 156 12 0.0 6 12 157 13 0.0 6 13 158 3 0.0 6 3 159 16 0.0 6 16 160 16 0.0 6 15 1 161 15 0.0 6 15 162 10 0.0 6 10 163 8 0.0 6 8 164 13 0.0 6 13 165 16 0.0 6 16 166 13 0.0 6 13 167 12 0.0 6 12 168 16 0.0 6 16 169 13 0.0 6 13 170 10 0.0 6 10 171 12 0.0 6 12 172 10 0.0 6 10 173 14 0.0 6 14 174 5 0.0 6 5 175 7 0.0 6 6 1 176 8 0.0 6 8 177 7 0.0 6 7 178 11 0.0 6 11 179 12 0.0 6 12 180 12 0.0 6 11 1 181 10 0.0 6 10 182 8 0.0 6 8 183 11 0.0 6 11 184 14 0.0 6 14 185 11 0.0 6 11 186 9 0.0 6 9 187 11 0.0 6 11 188 8 0.0 6 8 189 13 0.0 6 13 190 7 0.0 6 7 191 6 0.0 6 6 192 15 0.0 6 15 193 16 0.0 6 16 194 19 0.0 6 19 195 9 0.0 6 8 1 196 5 0.0 6 5 197 8 0.0 6 8 198 12 0.0 6 12 199 13 0.0 6 12 1 200 8 0.0 6 8 201 20 0.0 6 20 202 6 0.0 6 6 203 7 0.0 6 7 204 7 0.0 6 7 205 3 0.0 6 3 206 4 0.0 6 4 207 4 0.0 6 4 208 6 0.0 6 6 209 9 0.0 6 9 210 10 0.0 6 9 0 0 0 0 0 1 211 3 0.0 6 3 212 5 0.0 6 5 213 6 0.0 6 6 214 10 0.0 6 10 215 3 0.0 6 3 216 5 0.0 6 5 217 7 0.0 6 7 218 2 0.0 6 2 219 8 0.0 6 8 220 6 0.0 6 6 221 4 0.0 6 4 222 6 0.0 6 6 223 5 0.0 6 5 224 6 0.0 6 6 225 7 0.0 6 7 226 12 0.0 6 12 227 3 0.0 6 3 228 4 0.0 6 4 229 5 0.0 6 5 230 4 0.0 6 4 231 2 0.0 6 2 232 2 0.0 6 2 233 6 0.0 6 6 234 2 0.0 6 2 235 5 0.0 6 5 236 5 0.0 6 5 237 5 0.0 6 5 238 5 0.0 6 5 239 5 0.0 6 5 240 4 0.0 6 4 241 2 0.0 6 2 242 4 0.0 6 4 243 5 0.0 6 5 244 3 0.0 6 3 245 5 0.0 6 5 246 7 0.0 6 7 247 7 0.0 6 7 248 5 0.0 6 5 249 3 0.0 6 3 250 2 0.0 6 2 251 6 0.0 6 5 1 252 3 0.0 6 3 253 4 0.0 6 4 254 1 0.0 6 1 255 2 0.0 6 2 256 4 0.0 6 4 257 3 0.0 6 3 258 4 0.0 6 4 259 3 0.0 6 3 261 1 0.0 6 1 262 2 0.0 6 2 263 2 0.0 6 2 265 1 0.0 6 1 266 3 0.0 6 3 267 1 0.0 6 1 268 1 0.0 6 1 270 1 0.0 6 1 271 4 0.0 6 4 272 4 0.0 6 4 273 1 0.0 6 1 274 1 0.0 6 1 276 1 0.0 6 1 277 1 0.0 6 1 278 1 0.0 6 1 279 1 0.0 6 1 280 1 0.0 6 1 282 2 0.0 6 2 285 2 0.0 6 2 286 1 0.0 6 1 290 2 0.0 6 2 291 2 0.0 6 2 294 1 0.0 6 1 295 2 0.0 6 2 302 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 62056 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 2 15.5 1 2 7 1 3.9 2 1 8 2 1.0 2 2 9 1 0.2 2 1 10 1 0.1 3 1 11 2 0.0 3 2 12 8 0.0 3 8 13 6 0.0 3 6 14 7 0.0 4 6 1 15 1 0.0 4 1 16 7 0.0 4 4 3 17 21 0.0 5 17 2 2 18 1 0.0 5 1 19 40 0.0 5 36 4 20 70 0.0 6 68 2 21 40 0.0 6 40 22 33 0.0 6 33 23 14 0.0 6 14 24 19 0.0 6 18 1 25 15 0.0 6 15 26 17 0.0 6 17 27 19 0.0 6 17 2 28 12 0.0 6 10 2 29 13 0.0 6 12 1 30 11 0.0 6 9 2 31 16 0.0 6 15 1 32 15 0.0 6 13 2 33 10 0.0 6 10 34 12 0.0 6 10 2 35 14 0.0 6 13 1 36 12 0.0 6 9 2 0 0 0 0 1 37 9 0.0 6 8 1 38 10 0.0 6 10 39 14 0.0 6 12 1 0 0 0 0 1 40 7 0.0 6 7 41 11 0.0 6 10 1 42 3 0.0 6 2 1 43 14 0.0 6 12 1 0 1 44 9 0.0 6 7 2 45 19 0.0 6 18 1 46 12 0.0 6 12 47 13 0.0 6 11 0 0 0 0 0 2 48 20 0.0 6 19 1 49 16 0.0 6 14 2 50 18 0.0 6 16 2 51 14 0.0 6 11 2 1 52 21 0.0 6 19 1 1 53 16 0.0 6 15 1 54 19 0.0 6 15 3 0 0 1 55 22 0.0 6 22 56 14 0.0 6 14 57 18 0.0 6 15 1 0 0 0 0 2 58 16 0.0 6 16 59 15 0.0 6 13 2 60 22 0.0 6 21 1 61 26 0.0 6 24 1 0 0 0 0 1 62 27 0.0 6 23 4 63 28 0.0 6 26 2 64 20 0.0 6 18 0 1 0 0 0 1 65 15 0.0 6 15 66 37 0.0 6 37 67 19 0.0 6 17 1 0 1 68 27 0.0 6 25 2 69 38 0.0 6 35 2 1 70 122 0.0 6 117 4 1 71 48 0.0 6 45 2 0 0 1 72 72 0.0 6 67 5 73 27 0.0 6 26 1 74 49 0.0 6 46 2 0 1 75 36 0.0 6 35 1 76 35 0.0 6 32 3 77 33 0.0 6 31 2 78 35 0.0 6 33 1 0 0 0 0 1 79 40 0.0 6 38 2 80 41 0.0 6 41 81 47 0.0 6 46 1 82 51 0.0 6 46 3 1 0 0 0 1 83 61 0.0 6 54 6 0 1 84 53 0.0 6 46 6 0 0 1 85 46 0.0 6 44 2 86 77 0.0 6 74 3 87 49 0.0 6 46 2 0 0 0 0 1 88 41 0.0 6 37 3 1 89 51 0.0 6 47 4 90 52 0.0 6 49 2 0 0 0 0 1 91 54 0.0 6 53 1 92 49 0.0 6 47 2 93 62 0.0 6 60 2 94 65 0.0 6 61 3 1 95 62 0.0 6 55 6 0 1 96 94 0.0 6 88 5 0 1 97 65 0.0 6 63 2 98 78 0.0 6 77 1 99 71 0.0 6 66 4 0 1 100 76 0.0 6 73 2 0 0 0 1 101 80 0.0 6 78 2 102 76 0.0 6 74 2 103 69 0.0 6 69 104 99 0.0 6 92 4 0 2 1 105 68 0.0 6 68 106 87 0.0 6 83 2 0 0 1 0 1 107 160 0.0 6 156 2 1 0 1 108 113 0.0 6 110 3 109 77 0.0 6 73 4 110 110 0.0 6 107 3 111 114 0.0 6 111 2 0 0 0 0 1 112 116 0.0 6 111 3 1 0 0 1 113 145 0.0 6 141 3 0 0 0 1 114 129 0.0 6 123 6 115 155 0.0 6 149 4 1 0 0 1 116 136 0.0 6 132 4 117 146 0.0 6 141 5 118 178 0.0 6 170 7 1 119 143 0.0 6 139 4 120 176 0.0 6 168 5 0 0 1 1 1 121 197 0.0 6 192 4 0 0 0 1 122 173 0.0 6 169 4 123 171 0.0 6 170 1 124 177 0.0 6 171 4 1 0 0 1 125 193 0.0 6 189 4 126 189 0.0 6 186 3 127 248 0.0 6 236 10 2 128 268 0.0 6 262 3 2 0 0 1 129 302 0.0 6 296 5 0 0 0 1 130 265 0.0 6 262 2 0 0 0 0 1 131 301 0.0 6 294 6 0 0 0 1 132 317 0.0 6 312 4 0 0 0 0 1 133 338 0.0 6 325 12 0 0 0 0 1 134 420 0.0 6 407 12 0 0 0 0 1 135 382 0.0 6 374 8 136 457 0.0 6 448 8 0 0 0 0 1 137 522 0.0 6 514 7 0 1 138 626 0.0 6 617 8 1 139 716 0.0 6 703 12 0 0 0 0 1 140 763 0.0 6 753 8 0 1 0 0 1 141 653 0.0 6 647 6 142 729 0.0 6 716 11 0 2 143 837 0.0 6 828 9 144 854 0.0 6 845 8 0 0 0 1 145 955 0.0 6 936 13 0 3 1 2 146 811 0.0 6 799 11 0 1 147 926 0.0 6 907 14 1 0 1 2 1 148 937 0.0 6 929 5 1 1 0 0 1 149 965 0.0 6 955 9 0 0 1 150 946 0.0 6 932 13 0 0 0 0 1 151 1104 0.0 6 1090 12 0 0 1 0 1 152 1172 0.0 6 1145 22 0 1 2 1 1 153 1560 0.0 6 1536 22 1 0 0 1 154 1186 0.0 6 1166 14 3 0 1 2 155 1353 0.0 6 1325 25 1 0 0 1 1 156 1475 0.0 6 1457 18 157 1083 0.0 6 1077 6 158 1174 0.0 6 1155 16 2 1 159 1051 0.0 6 1038 11 0 0 1 0 1 160 1195 0.0 6 1176 17 0 0 1 1 161 1082 0.0 6 1070 10 0 1 0 1 162 976 0.0 6 958 15 0 0 3 163 916 0.0 6 901 10 2 0 2 0 1 164 850 0.0 6 837 9 2 0 0 2 165 771 0.0 6 764 5 0 0 0 1 1 166 830 0.0 6 822 7 1 167 833 0.0 6 823 9 0 0 0 0 1 168 728 0.0 6 717 6 1 0 2 0 2 169 928 0.0 6 918 7 2 0 1 170 861 0.0 6 853 7 0 0 0 1 171 937 0.0 6 930 4 0 2 0 0 1 172 849 0.0 6 835 9 1 3 1 173 836 0.0 6 826 9 0 1 174 970 0.0 6 959 8 1 0 1 1 175 913 0.0 6 904 6 1 2 176 962 0.0 6 954 7 0 0 1 177 743 0.0 6 729 12 0 0 1 0 1 178 708 0.0 6 696 10 0 1 0 0 1 179 773 0.0 6 763 8 1 0 0 0 1 180 634 0.0 6 632 2 181 632 0.0 6 624 7 1 182 507 0.0 6 500 6 0 0 0 1 183 528 0.0 6 523 5 184 445 0.0 6 442 3 185 435 0.0 6 430 5 186 618 0.0 6 612 5 0 0 0 1 187 800 0.0 6 795 5 188 667 0.0 6 660 5 0 1 0 1 189 487 0.0 6 484 1 0 0 2 190 488 0.0 6 482 6 191 452 0.0 6 446 5 0 0 0 0 1 192 405 0.0 6 403 1 1 193 392 0.0 6 387 4 1 194 395 0.0 6 394 0 0 0 1 195 309 0.0 6 307 2 196 303 0.0 6 297 6 197 237 0.0 6 236 0 0 0 0 0 1 198 217 0.0 6 217 199 344 0.0 6 341 3 200 228 0.0 6 225 3 201 222 0.0 6 220 2 202 212 0.0 6 209 2 0 1 203 185 0.0 6 183 1 0 0 0 1 204 143 0.0 6 140 3 205 156 0.0 6 156 206 136 0.0 6 134 2 207 123 0.0 6 123 208 118 0.0 6 116 1 0 0 1 209 109 0.0 6 107 1 0 0 1 210 100 0.0 6 95 3 0 0 0 0 2 211 121 0.0 6 121 212 100 0.0 6 98 2 213 81 0.0 6 80 1 214 70 0.0 6 69 1 215 67 0.0 6 67 216 81 0.0 6 81 217 95 0.0 6 94 0 1 218 57 0.0 6 57 219 79 0.0 6 79 220 75 0.0 6 72 3 221 37 0.0 6 34 3 222 37 0.0 6 36 1 223 38 0.0 6 38 224 46 0.0 6 46 225 61 0.0 6 58 3 226 42 0.0 6 42 227 36 0.0 6 35 0 1 228 21 0.0 6 21 229 31 0.0 6 31 230 17 0.0 6 17 231 23 0.0 6 23 232 18 0.0 6 16 2 233 18 0.0 6 17 1 234 8 0.0 6 8 235 18 0.0 6 17 1 236 1 0.0 6 1 237 3 0.0 6 3 238 2 0.0 6 2 239 6 0.0 6 6 240 4 0.0 6 4 241 6 0.0 6 6 242 3 0.0 6 3 243 8 0.0 6 8 244 9 0.0 6 9 245 3 0.0 6 3 246 1 0.0 6 1 247 3 0.0 6 2 0 0 0 0 1 248 5 0.0 6 5 249 2 0.0 6 2 250 1 0.0 6 1 251 2 0.0 6 2 252 1 0.0 6 1 253 1 0.0 6 1 254 1 0.0 6 1 279 1 0.0 6 1 280 1 0.0 6 1 281 7 0.0 6 7 282 74 0.0 6 73 0 0 0 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1245 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 54 1 0.0 6 1 55 1 0.0 6 1 60 1 0.0 6 1 66 5 0.0 6 5 68 1 0.0 6 1 69 6 0.0 6 6 70 13 0.0 6 13 71 12 0.0 6 12 72 11 0.0 6 11 73 9 0.0 6 9 74 11 0.0 6 10 0 0 1 75 20 0.0 6 20 76 11 0.0 6 11 77 16 0.0 6 16 78 18 0.0 6 18 79 10 0.0 6 10 80 9 0.0 6 9 81 7 0.0 6 7 82 9 0.0 6 9 83 9 0.0 6 9 84 5 0.0 6 5 85 6 0.0 6 6 86 9 0.0 6 9 87 8 0.0 6 8 88 8 0.0 6 7 0 0 0 1 89 11 0.0 6 11 90 13 0.0 6 12 0 0 0 1 91 7 0.0 6 7 92 10 0.0 6 10 93 5 0.0 6 5 94 7 0.0 6 7 95 10 0.0 6 10 96 8 0.0 6 8 97 7 0.0 6 7 98 7 0.0 6 7 99 5 0.0 6 5 100 8 0.0 6 8 101 7 0.0 6 7 102 9 0.0 6 9 103 17 0.0 6 17 104 10 0.0 6 10 105 12 0.0 6 12 106 17 0.0 6 17 107 28 0.0 6 28 108 14 0.0 6 14 109 15 0.0 6 15 110 11 0.0 6 11 111 6 0.0 6 6 112 16 0.0 6 16 113 5 0.0 6 5 114 12 0.0 6 12 115 4 0.0 6 4 116 4 0.0 6 4 117 3 0.0 6 3 118 7 0.0 6 7 119 6 0.0 6 6 120 3 0.0 6 3 121 8 0.0 6 8 122 13 0.0 6 13 123 7 0.0 6 7 124 6 0.0 6 6 125 10 0.0 6 10 126 8 0.0 6 8 127 10 0.0 6 10 128 11 0.0 6 11 129 6 0.0 6 6 130 12 0.0 6 12 131 12 0.0 6 12 132 9 0.0 6 9 133 6 0.0 6 6 134 15 0.0 6 15 135 5 0.0 6 5 136 19 0.0 6 19 137 15 0.0 6 15 138 16 0.0 6 16 139 7 0.0 6 7 140 9 0.0 6 9 141 11 0.0 6 11 142 13 0.0 6 13 143 9 0.0 6 9 144 8 0.0 6 8 145 1 0.0 6 1 146 4 0.0 6 4 147 5 0.0 6 5 148 9 0.0 6 9 149 4 0.0 6 4 150 4 0.0 6 4 151 8 0.0 6 8 152 4 0.0 6 4 153 13 0.0 6 13 154 7 0.0 6 7 155 16 0.0 6 16 156 9 0.0 6 8 1 157 6 0.0 6 6 158 18 0.0 6 18 159 18 0.0 6 18 160 14 0.0 6 14 161 14 0.0 6 14 162 2 0.0 6 2 163 9 0.0 6 9 164 15 0.0 6 15 165 10 0.0 6 10 166 23 0.0 6 23 167 14 0.0 6 14 168 10 0.0 6 10 169 6 0.0 6 6 170 12 0.0 6 12 171 11 0.0 6 11 172 11 0.0 6 11 173 5 0.0 6 5 174 6 0.0 6 6 175 8 0.0 6 8 176 7 0.0 6 5 0 0 0 2 177 14 0.0 6 14 178 3 0.0 6 3 179 12 0.0 6 12 180 4 0.0 6 4 181 6 0.0 6 6 182 13 0.0 6 13 183 7 0.0 6 7 184 10 0.0 6 9 0 0 0 1 185 9 0.0 6 8 1 186 8 0.0 6 8 187 6 0.0 6 6 188 6 0.0 6 6 189 3 0.0 6 3 190 2 0.0 6 2 191 2 0.0 6 2 192 5 0.0 6 5 193 4 0.0 6 4 194 7 0.0 6 7 195 4 0.0 6 4 197 1 0.0 6 1 198 3 0.0 6 3 199 2 0.0 6 2 200 6 0.0 6 6 201 1 0.0 6 1 202 1 0.0 6 1 203 2 0.0 6 2 204 1 0.0 6 1 207 3 0.0 6 3 208 1 0.0 6 1 209 3 0.0 6 3 210 4 0.0 6 4 211 2 0.0 6 2 212 1 0.0 6 1 213 3 0.0 6 3 215 4 0.0 6 4 216 3 0.0 6 3 219 1 0.0 6 1 224 1 0.0 6 1 225 2 0.0 6 2 227 1 0.0 6 1 229 1 0.0 6 1 230 2 0.0 6 2 238 2 0.0 6 2 246 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_12_169_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_12_396_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_12_169_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_12_396_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.32 s (68 µs/read; 0.89 M reads/minute). === Summary === Total read pairs processed: 34,250 Read 1 with adapter: 34,249 (100.0%) Read 2 with adapter: 34,214 (99.9%) == Read fate breakdown == Pairs that were too short: 2 (0.0%) Pairs discarded as untrimmed: 37 (0.1%) Pairs written (passing filters): 34,211 (99.9%) Total basepairs processed: 15,860,168 bp Read 1: 8,906,372 bp Read 2: 6,953,796 bp Total written (filtered): 2,105,471 bp (13.3%) Read 1: 1,035,408 bp Read 2: 1,070,063 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 34104 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 8 1 0.5 2 1 9 3 0.1 2 3 15 2 0.0 4 2 19 1 0.0 5 1 20 16 0.0 6 16 21 11 0.0 6 10 1 22 11 0.0 6 11 23 14 0.0 6 14 24 4 0.0 6 4 25 11 0.0 6 11 26 3 0.0 6 3 27 2 0.0 6 2 28 4 0.0 6 4 29 4 0.0 6 4 30 3 0.0 6 3 31 2 0.0 6 2 32 2 0.0 6 2 33 4 0.0 6 4 34 2 0.0 6 2 37 1 0.0 6 1 38 1 0.0 6 1 39 1 0.0 6 0 1 40 3 0.0 6 3 41 1 0.0 6 1 43 1 0.0 6 1 44 5 0.0 6 5 46 2 0.0 6 1 1 47 1 0.0 6 1 48 2 0.0 6 2 49 1 0.0 6 1 50 1 0.0 6 1 51 1 0.0 6 1 55 1 0.0 6 1 56 2 0.0 6 1 0 1 57 1 0.0 6 1 58 3 0.0 6 3 59 2 0.0 6 2 61 1 0.0 6 1 62 2 0.0 6 2 63 1 0.0 6 1 65 1 0.0 6 1 66 1 0.0 6 1 67 1 0.0 6 1 68 2 0.0 6 2 69 1 0.0 6 1 70 1 0.0 6 1 71 1 0.0 6 1 72 1 0.0 6 1 74 3 0.0 6 2 1 75 1 0.0 6 1 76 1 0.0 6 1 77 4 0.0 6 4 78 5 0.0 6 5 79 4 0.0 6 4 81 2 0.0 6 2 82 3 0.0 6 3 83 4 0.0 6 4 84 2 0.0 6 2 85 8 0.0 6 8 86 7 0.0 6 7 87 9 0.0 6 8 1 88 1 0.0 6 1 89 2 0.0 6 2 90 3 0.0 6 3 91 5 0.0 6 5 92 3 0.0 6 3 93 2 0.0 6 1 1 94 1 0.0 6 0 1 95 1 0.0 6 1 96 4 0.0 6 4 97 5 0.0 6 5 98 2 0.0 6 2 99 2 0.0 6 2 100 2 0.0 6 2 101 4 0.0 6 4 102 6 0.0 6 6 103 3 0.0 6 3 104 8 0.0 6 7 1 105 5 0.0 6 5 106 6 0.0 6 6 107 4 0.0 6 4 108 7 0.0 6 7 109 3 0.0 6 3 110 5 0.0 6 5 111 9 0.0 6 9 112 13 0.0 6 13 113 5 0.0 6 3 2 114 10 0.0 6 10 115 3 0.0 6 3 116 8 0.0 6 8 117 6 0.0 6 6 118 6 0.0 6 5 0 1 119 12 0.0 6 11 1 120 4 0.0 6 4 121 13 0.0 6 12 1 122 11 0.0 6 11 123 16 0.0 6 15 1 124 11 0.0 6 11 125 11 0.0 6 11 126 15 0.0 6 15 127 11 0.0 6 11 128 11 0.0 6 11 129 12 0.0 6 12 130 11 0.0 6 11 131 10 0.0 6 10 132 20 0.0 6 20 133 15 0.0 6 15 134 12 0.0 6 12 135 14 0.0 6 14 136 9 0.0 6 9 137 21 0.0 6 21 138 14 0.0 6 14 139 27 0.0 6 27 140 16 0.0 6 16 141 34 0.0 6 34 142 95 0.0 6 95 143 259 0.0 6 259 144 234 0.0 6 234 145 251 0.0 6 251 146 288 0.0 6 288 147 259 0.0 6 259 148 306 0.0 6 305 1 149 317 0.0 6 317 150 286 0.0 6 285 1 151 249 0.0 6 249 152 312 0.0 6 311 1 153 202 0.0 6 202 154 180 0.0 6 180 155 170 0.0 6 170 156 139 0.0 6 139 157 154 0.0 6 154 158 139 0.0 6 139 159 170 0.0 6 170 160 179 0.0 6 179 161 152 0.0 6 152 162 150 0.0 6 150 163 123 0.0 6 123 164 110 0.0 6 110 165 103 0.0 6 102 1 166 111 0.0 6 111 167 104 0.0 6 104 168 110 0.0 6 110 169 84 0.0 6 83 1 170 157 0.0 6 155 2 171 124 0.0 6 124 172 142 0.0 6 137 5 173 142 0.0 6 141 1 174 132 0.0 6 132 175 91 0.0 6 90 1 176 86 0.0 6 86 177 89 0.0 6 88 1 178 79 0.0 6 77 2 179 65 0.0 6 64 1 180 130 0.0 6 130 181 81 0.0 6 80 1 182 56 0.0 6 56 183 56 0.0 6 56 184 106 0.0 6 105 0 1 185 89 0.0 6 88 1 186 117 0.0 6 114 2 0 0 1 187 80 0.0 6 79 1 188 112 0.0 6 111 1 189 119 0.0 6 115 4 190 98 0.0 6 96 2 191 69 0.0 6 69 192 84 0.0 6 79 5 193 91 0.0 6 90 1 194 89 0.0 6 89 195 70 0.0 6 70 196 97 0.0 6 96 1 197 91 0.0 6 85 6 198 142 0.0 6 141 1 199 120 0.0 6 120 200 129 0.0 6 129 201 101 0.0 6 98 3 202 98 0.0 6 96 2 203 143 0.0 6 141 2 204 122 0.0 6 119 3 205 132 0.0 6 127 4 1 206 171 0.0 6 167 4 207 146 0.0 6 140 6 208 249 0.0 6 245 4 209 145 0.0 6 144 1 210 170 0.0 6 168 2 211 183 0.0 6 181 2 212 108 0.0 6 104 4 213 199 0.0 6 190 7 2 214 226 0.0 6 224 2 215 150 0.0 6 145 5 216 259 0.0 6 251 8 217 196 0.0 6 196 218 182 0.0 6 177 5 219 188 0.0 6 185 3 220 246 0.0 6 235 11 221 200 0.0 6 197 3 222 199 0.0 6 195 4 223 200 0.0 6 195 5 224 227 0.0 6 223 4 225 214 0.0 6 213 1 226 281 0.0 6 276 5 227 262 0.0 6 257 5 228 201 0.0 6 196 5 229 200 0.0 6 196 4 230 169 0.0 6 166 2 1 231 202 0.0 6 199 3 232 210 0.0 6 208 2 233 202 0.0 6 196 6 234 216 0.0 6 210 6 235 210 0.0 6 202 8 236 246 0.0 6 237 9 237 152 0.0 6 150 2 238 142 0.0 6 140 2 239 387 0.0 6 378 9 240 298 0.0 6 293 5 241 126 0.0 6 122 4 242 143 0.0 6 140 3 243 145 0.0 6 144 1 244 106 0.0 6 104 2 245 100 0.0 6 96 3 1 246 149 0.0 6 143 6 247 234 0.0 6 231 3 248 129 0.0 6 127 2 249 189 0.0 6 182 7 250 170 0.0 6 165 5 251 181 0.0 6 170 11 252 334 0.0 6 331 3 253 186 0.0 6 179 7 254 170 0.0 6 167 3 255 163 0.0 6 155 8 256 291 0.0 6 285 6 257 352 0.0 6 340 12 258 265 0.0 6 260 4 0 1 259 404 0.0 6 394 10 260 270 0.0 6 259 11 261 439 0.0 6 432 7 262 192 0.0 6 184 8 263 296 0.0 6 293 3 264 143 0.0 6 140 3 265 129 0.0 6 127 2 266 227 0.0 6 222 5 267 261 0.0 6 254 5 2 268 153 0.0 6 150 3 269 245 0.0 6 236 9 270 330 0.0 6 324 6 271 274 0.0 6 267 7 272 260 0.0 6 254 6 273 172 0.0 6 166 5 1 274 201 0.0 6 197 4 275 272 0.0 6 263 9 276 543 0.0 6 535 8 277 614 0.0 6 600 14 278 882 0.0 6 864 18 279 1293 0.0 6 1274 17 2 280 3203 0.0 6 3124 78 1 281 2988 0.0 6 2945 43 282 7 0.0 6 7 283 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 145 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 2 0.0 6 2 70 1 0.0 6 1 71 6 0.0 6 6 72 1 0.0 6 1 73 4 0.0 6 4 74 6 0.0 6 6 75 6 0.0 6 6 76 2 0.0 6 2 77 7 0.0 6 7 78 1 0.0 6 1 79 9 0.0 6 9 80 4 0.0 6 4 81 5 0.0 6 5 82 5 0.0 6 5 83 1 0.0 6 1 85 5 0.0 6 5 86 2 0.0 6 2 87 4 0.0 6 4 89 3 0.0 6 3 90 2 0.0 6 2 93 1 0.0 6 1 94 2 0.0 6 2 95 1 0.0 6 1 96 1 0.0 6 1 98 1 0.0 6 1 99 2 0.0 6 2 100 1 0.0 6 1 105 2 0.0 6 2 106 3 0.0 6 3 107 1 0.0 6 1 110 1 0.0 6 1 111 1 0.0 6 1 112 1 0.0 6 1 113 1 0.0 6 1 116 1 0.0 6 1 121 3 0.0 6 3 123 1 0.0 6 1 125 1 0.0 6 1 126 1 0.0 6 1 127 1 0.0 6 1 128 1 0.0 6 1 131 1 0.0 6 1 132 2 0.0 6 2 136 2 0.0 6 2 139 1 0.0 6 1 140 1 0.0 6 1 143 1 0.0 6 1 145 1 0.0 6 1 149 2 0.0 6 2 153 2 0.0 6 2 154 1 0.0 6 1 157 1 0.0 6 1 161 1 0.0 6 1 162 1 0.0 6 1 164 1 0.0 6 1 165 1 0.0 6 1 166 1 0.0 6 1 171 1 0.0 6 1 172 1 0.0 6 1 173 1 0.0 6 1 175 1 0.0 6 1 179 1 0.0 6 1 183 2 0.0 6 2 185 1 0.0 6 1 186 1 0.0 6 1 192 1 0.0 6 1 201 1 0.0 6 1 203 1 0.0 6 1 204 1 0.0 6 1 207 1 0.0 6 1 209 1 0.0 6 1 213 1 0.0 6 1 218 1 0.0 6 1 226 1 0.0 6 1 246 1 0.0 6 1 256 1 0.0 6 1 259 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 34003 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 535.2 0 0 1 6 1 8.4 1 1 11 2 0.0 3 2 12 4 0.0 3 4 13 7 0.0 3 6 1 14 7 0.0 4 7 15 1 0.0 4 1 16 2 0.0 4 2 17 19 0.0 5 17 2 18 7 0.0 5 7 19 6 0.0 5 6 20 17 0.0 6 17 21 19 0.0 6 18 1 22 14 0.0 6 13 1 23 18 0.0 6 15 3 24 7 0.0 6 5 2 25 8 0.0 6 7 1 26 15 0.0 6 14 1 27 3 0.0 6 3 28 6 0.0 6 5 1 29 4 0.0 6 4 30 6 0.0 6 5 1 31 5 0.0 6 5 32 2 0.0 6 2 34 3 0.0 6 3 35 3 0.0 6 3 36 2 0.0 6 2 37 4 0.0 6 4 38 7 0.0 6 7 39 1 0.0 6 1 40 4 0.0 6 4 41 7 0.0 6 7 42 1 0.0 6 1 43 5 0.0 6 5 44 13 0.0 6 13 45 7 0.0 6 7 46 14 0.0 6 13 1 47 11 0.0 6 11 48 12 0.0 6 11 1 49 18 0.0 6 17 1 50 13 0.0 6 11 1 0 0 1 51 8 0.0 6 8 52 19 0.0 6 17 2 53 18 0.0 6 17 1 54 7 0.0 6 7 55 17 0.0 6 17 56 9 0.0 6 8 1 57 8 0.0 6 7 1 58 11 0.0 6 10 1 59 9 0.0 6 8 1 60 7 0.0 6 6 0 1 61 11 0.0 6 10 1 62 8 0.0 6 7 1 63 8 0.0 6 7 1 64 7 0.0 6 7 65 13 0.0 6 13 66 16 0.0 6 14 2 67 35 0.0 6 33 1 0 0 1 68 58 0.0 6 57 0 0 1 69 24 0.0 6 21 3 70 41 0.0 6 40 1 71 12 0.0 6 11 1 72 18 0.0 6 17 1 73 16 0.0 6 14 2 74 16 0.0 6 16 75 17 0.0 6 14 3 76 17 0.0 6 17 77 11 0.0 6 9 2 78 22 0.0 6 22 79 13 0.0 6 13 80 23 0.0 6 23 81 19 0.0 6 18 1 82 25 0.0 6 24 1 83 47 0.0 6 46 1 84 46 0.0 6 45 1 85 28 0.0 6 24 4 86 18 0.0 6 16 2 87 30 0.0 6 26 3 0 0 0 1 88 12 0.0 6 12 89 14 0.0 6 14 90 17 0.0 6 13 4 91 32 0.0 6 31 0 1 92 22 0.0 6 19 2 1 93 23 0.0 6 21 2 94 38 0.0 6 36 2 95 17 0.0 6 17 96 23 0.0 6 22 0 1 97 23 0.0 6 23 98 28 0.0 6 27 0 0 0 1 99 20 0.0 6 18 2 100 18 0.0 6 17 1 101 30 0.0 6 27 3 102 31 0.0 6 29 2 103 40 0.0 6 40 104 33 0.0 6 32 1 105 48 0.0 6 48 106 28 0.0 6 26 2 107 25 0.0 6 23 1 0 0 1 108 35 0.0 6 35 109 28 0.0 6 27 1 110 45 0.0 6 42 3 111 26 0.0 6 26 112 46 0.0 6 46 113 42 0.0 6 42 114 36 0.0 6 35 1 115 38 0.0 6 38 116 49 0.0 6 46 3 117 34 0.0 6 34 118 49 0.0 6 46 1 1 1 119 62 0.0 6 59 2 0 0 1 120 49 0.0 6 48 1 121 48 0.0 6 45 3 122 42 0.0 6 42 123 63 0.0 6 61 2 124 62 0.0 6 61 1 125 54 0.0 6 52 0 1 0 0 1 126 82 0.0 6 79 3 127 83 0.0 6 81 2 128 94 0.0 6 94 129 88 0.0 6 86 1 1 130 101 0.0 6 97 4 131 144 0.0 6 140 4 132 128 0.0 6 126 2 133 110 0.0 6 108 1 0 0 1 134 146 0.0 6 142 4 135 200 0.0 6 193 7 136 228 0.0 6 220 8 137 290 0.0 6 289 1 138 286 0.0 6 282 3 0 0 1 139 353 0.0 6 349 2 2 140 450 0.0 6 439 9 1 1 141 388 0.0 6 378 9 0 0 0 1 142 580 0.0 6 569 10 0 0 0 1 143 492 0.0 6 485 7 144 565 0.0 6 554 11 145 542 0.0 6 532 9 0 1 146 498 0.0 6 489 9 147 345 0.0 6 339 6 148 360 0.0 6 357 3 149 398 0.0 6 393 5 150 394 0.0 6 387 7 151 554 0.0 6 550 4 152 383 0.0 6 375 8 153 367 0.0 6 365 2 154 419 0.0 6 409 9 0 0 0 0 1 155 275 0.0 6 272 2 1 156 309 0.0 6 308 1 157 325 0.0 6 317 8 158 338 0.0 6 333 4 0 0 1 159 231 0.0 6 225 6 160 286 0.0 6 283 3 161 241 0.0 6 238 3 162 276 0.0 6 270 6 163 228 0.0 6 225 3 164 275 0.0 6 271 4 165 270 0.0 6 264 6 166 296 0.0 6 289 5 2 167 307 0.0 6 299 8 168 407 0.0 6 400 5 1 0 1 169 231 0.0 6 228 3 170 288 0.0 6 283 5 171 372 0.0 6 366 6 172 262 0.0 6 258 2 1 1 173 333 0.0 6 325 8 174 315 0.0 6 310 5 175 455 0.0 6 442 11 0 0 2 176 245 0.0 6 240 4 0 0 0 1 177 347 0.0 6 339 7 1 178 241 0.0 6 240 1 179 265 0.0 6 256 7 1 0 0 0 1 180 302 0.0 6 293 8 0 0 1 181 315 0.0 6 310 4 1 182 159 0.0 6 156 3 183 303 0.0 6 296 7 184 222 0.0 6 214 8 185 204 0.0 6 198 4 2 186 471 0.0 6 463 8 187 292 0.0 6 281 10 1 188 364 0.0 6 359 5 189 362 0.0 6 348 14 190 539 0.0 6 527 11 1 191 453 0.0 6 446 7 192 337 0.0 6 329 8 193 345 0.0 6 332 13 194 340 0.0 6 329 11 195 397 0.0 6 388 9 196 741 0.0 6 717 24 197 293 0.0 6 281 12 198 461 0.0 6 449 11 1 199 440 0.0 6 430 10 200 281 0.0 6 271 10 201 379 0.0 6 371 8 202 283 0.0 6 275 7 1 203 186 0.0 6 179 7 204 274 0.0 6 271 3 205 209 0.0 6 203 5 1 206 178 0.0 6 171 7 207 222 0.0 6 213 9 208 359 0.0 6 354 5 209 88 0.0 6 86 2 210 177 0.0 6 171 6 211 262 0.0 6 254 8 212 251 0.0 6 249 2 213 116 0.0 6 113 3 214 172 0.0 6 168 4 215 187 0.0 6 177 9 1 216 273 0.0 6 266 7 217 138 0.0 6 135 3 218 135 0.0 6 133 2 219 142 0.0 6 140 2 220 72 0.0 6 69 3 221 171 0.0 6 167 4 222 156 0.0 6 147 8 1 223 175 0.0 6 168 7 224 104 0.0 6 103 1 225 107 0.0 6 105 2 226 164 0.0 6 158 6 227 353 0.0 6 344 7 1 0 1 228 188 0.0 6 184 4 229 199 0.0 6 193 5 0 0 0 0 1 230 515 0.0 6 507 8 231 86 0.0 6 85 1 232 259 0.0 6 254 5 233 93 0.0 6 91 2 234 117 0.0 6 114 3 235 64 0.0 6 63 1 236 39 0.0 6 36 3 237 63 0.0 6 63 238 47 0.0 6 44 3 239 18 0.0 6 18 240 23 0.0 6 23 241 31 0.0 6 30 1 242 50 0.0 6 49 1 243 34 0.0 6 34 244 20 0.0 6 17 3 245 44 0.0 6 43 1 246 32 0.0 6 32 247 20 0.0 6 19 1 248 29 0.0 6 28 1 249 44 0.0 6 43 1 250 17 0.0 6 16 1 251 24 0.0 6 23 1 252 24 0.0 6 24 253 10 0.0 6 10 254 47 0.0 6 46 1 255 6 0.0 6 6 256 9 0.0 6 9 258 1 0.0 6 1 261 3 0.0 6 2 1 279 16 0.0 6 16 280 43 0.0 6 42 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 211 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 2 0.0 6 2 68 1 0.0 6 1 70 2 0.0 6 2 71 2 0.0 6 2 72 5 0.0 6 5 73 9 0.0 6 9 74 8 0.0 6 8 75 5 0.0 6 5 76 6 0.0 6 6 77 6 0.0 6 6 78 9 0.0 6 9 79 5 0.0 6 5 80 4 0.0 6 4 81 5 0.0 6 5 82 3 0.0 6 3 83 2 0.0 6 2 84 4 0.0 6 4 85 3 0.0 6 3 86 5 0.0 6 5 87 2 0.0 6 2 88 3 0.0 6 3 89 3 0.0 6 3 90 5 0.0 6 5 91 2 0.0 6 2 92 6 0.0 6 6 94 1 0.0 6 1 95 7 0.0 6 7 96 3 0.0 6 2 1 97 2 0.0 6 2 98 1 0.0 6 1 99 1 0.0 6 1 100 3 0.0 6 3 101 4 0.0 6 4 102 1 0.0 6 1 103 1 0.0 6 1 104 1 0.0 6 1 105 1 0.0 6 1 106 2 0.0 6 2 107 3 0.0 6 3 108 4 0.0 6 4 109 5 0.0 6 5 110 1 0.0 6 1 111 1 0.0 6 1 113 1 0.0 6 1 114 1 0.0 6 1 117 1 0.0 6 1 118 2 0.0 6 2 119 2 0.0 6 2 120 2 0.0 6 2 121 1 0.0 6 1 122 3 0.0 6 3 124 2 0.0 6 2 125 3 0.0 6 3 126 1 0.0 6 1 129 1 0.0 6 1 130 2 0.0 6 2 131 1 0.0 6 1 132 1 0.0 6 1 133 1 0.0 6 1 134 2 0.0 6 2 135 1 0.0 6 1 136 2 0.0 6 2 137 4 0.0 6 4 138 3 0.0 6 3 140 1 0.0 6 1 141 2 0.0 6 2 142 1 0.0 6 1 143 1 0.0 6 1 147 1 0.0 6 1 150 1 0.0 6 1 152 1 0.0 6 1 153 1 0.0 6 1 154 1 0.0 6 1 155 1 0.0 6 1 161 2 0.0 6 2 163 1 0.0 6 1 164 1 0.0 6 1 165 1 0.0 6 1 171 1 0.0 6 1 172 2 0.0 6 2 175 2 0.0 6 2 180 1 0.0 6 1 184 1 0.0 6 1 186 1 0.0 6 1 194 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_13_170_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_13_397_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_13_170_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_13_397_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.55 s (78 µs/read; 0.77 M reads/minute). === Summary === Total read pairs processed: 32,523 Read 1 with adapter: 32,520 (100.0%) Read 2 with adapter: 32,509 (100.0%) == Read fate breakdown == Pairs that were too short: 2 (0.0%) Pairs discarded as untrimmed: 17 (0.1%) Pairs written (passing filters): 32,504 (99.9%) Total basepairs processed: 15,166,655 bp Read 1: 8,408,502 bp Read 2: 6,758,153 bp Total written (filtered): 1,971,878 bp (13.0%) Read 1: 987,692 bp Read 2: 984,186 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 32304 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 508.2 0 0 1 6 1 7.9 1 0 0 1 12 1 0.0 3 1 20 18 0.0 6 18 21 10 0.0 6 10 22 11 0.0 6 11 23 9 0.0 6 9 24 5 0.0 6 4 1 25 7 0.0 6 7 26 1 0.0 6 1 27 3 0.0 6 3 28 4 0.0 6 3 1 29 3 0.0 6 3 30 1 0.0 6 1 31 2 0.0 6 2 32 3 0.0 6 3 33 1 0.0 6 1 34 3 0.0 6 3 35 1 0.0 6 1 36 2 0.0 6 2 37 4 0.0 6 4 38 1 0.0 6 1 39 2 0.0 6 1 1 40 1 0.0 6 1 41 1 0.0 6 1 42 1 0.0 6 1 44 2 0.0 6 2 45 5 0.0 6 5 46 3 0.0 6 3 47 4 0.0 6 4 48 1 0.0 6 1 49 3 0.0 6 3 50 1 0.0 6 1 51 2 0.0 6 2 52 6 0.0 6 5 0 0 0 0 1 53 3 0.0 6 3 54 3 0.0 6 3 55 1 0.0 6 1 56 2 0.0 6 2 57 1 0.0 6 1 58 1 0.0 6 1 60 1 0.0 6 1 62 2 0.0 6 2 63 2 0.0 6 2 64 2 0.0 6 2 65 1 0.0 6 1 68 2 0.0 6 1 1 69 1 0.0 6 1 70 1 0.0 6 1 73 4 0.0 6 4 74 1 0.0 6 1 76 2 0.0 6 2 77 2 0.0 6 2 78 2 0.0 6 1 1 79 1 0.0 6 1 80 2 0.0 6 2 81 1 0.0 6 1 83 1 0.0 6 1 84 1 0.0 6 1 86 2 0.0 6 2 87 4 0.0 6 4 88 1 0.0 6 1 90 3 0.0 6 3 91 3 0.0 6 3 92 2 0.0 6 2 93 1 0.0 6 1 94 1 0.0 6 1 95 1 0.0 6 1 97 5 0.0 6 5 98 3 0.0 6 3 99 2 0.0 6 2 100 4 0.0 6 4 101 5 0.0 6 5 102 3 0.0 6 2 1 103 5 0.0 6 5 104 3 0.0 6 3 105 6 0.0 6 6 106 4 0.0 6 4 107 12 0.0 6 12 108 10 0.0 6 10 109 6 0.0 6 6 110 8 0.0 6 8 111 6 0.0 6 6 112 7 0.0 6 7 113 2 0.0 6 2 114 10 0.0 6 9 1 115 4 0.0 6 4 116 9 0.0 6 9 117 5 0.0 6 5 118 10 0.0 6 10 119 6 0.0 6 6 120 9 0.0 6 9 121 6 0.0 6 6 122 7 0.0 6 7 123 5 0.0 6 5 124 13 0.0 6 13 125 11 0.0 6 11 126 15 0.0 6 15 127 8 0.0 6 8 128 14 0.0 6 14 129 8 0.0 6 7 1 130 10 0.0 6 10 131 18 0.0 6 18 132 18 0.0 6 18 133 13 0.0 6 13 134 13 0.0 6 13 135 11 0.0 6 11 136 21 0.0 6 21 137 16 0.0 6 16 138 28 0.0 6 28 139 22 0.0 6 22 140 32 0.0 6 32 141 142 0.0 6 142 142 264 0.0 6 264 143 364 0.0 6 364 144 282 0.0 6 282 145 232 0.0 6 232 146 303 0.0 6 303 147 289 0.0 6 289 148 278 0.0 6 278 149 223 0.0 6 223 150 204 0.0 6 203 1 151 277 0.0 6 277 152 218 0.0 6 216 1 1 153 185 0.0 6 185 154 134 0.0 6 134 155 137 0.0 6 136 1 156 134 0.0 6 134 157 124 0.0 6 123 1 158 147 0.0 6 147 159 154 0.0 6 154 160 149 0.0 6 148 1 161 189 0.0 6 188 1 162 139 0.0 6 139 163 105 0.0 6 105 164 134 0.0 6 134 165 118 0.0 6 118 166 90 0.0 6 90 167 136 0.0 6 136 168 98 0.0 6 98 169 114 0.0 6 113 1 170 119 0.0 6 119 171 106 0.0 6 105 1 172 122 0.0 6 122 173 115 0.0 6 115 174 116 0.0 6 115 1 175 104 0.0 6 104 176 89 0.0 6 89 177 96 0.0 6 96 178 90 0.0 6 90 179 154 0.0 6 154 180 110 0.0 6 110 181 86 0.0 6 86 182 95 0.0 6 94 1 183 92 0.0 6 92 184 103 0.0 6 101 2 185 102 0.0 6 101 1 186 79 0.0 6 78 1 187 82 0.0 6 81 1 188 105 0.0 6 105 189 99 0.0 6 99 190 79 0.0 6 79 191 123 0.0 6 123 192 115 0.0 6 114 0 0 0 0 0 1 193 104 0.0 6 103 1 194 121 0.0 6 119 2 195 112 0.0 6 111 1 196 140 0.0 6 140 197 149 0.0 6 147 2 198 162 0.0 6 161 1 199 159 0.0 6 159 200 156 0.0 6 156 201 124 0.0 6 123 1 202 117 0.0 6 117 203 200 0.0 6 199 1 204 120 0.0 6 119 1 205 125 0.0 6 122 3 206 120 0.0 6 120 207 130 0.0 6 128 2 208 159 0.0 6 158 1 209 172 0.0 6 171 1 210 168 0.0 6 166 2 211 153 0.0 6 152 1 212 157 0.0 6 156 1 213 132 0.0 6 129 2 1 214 205 0.0 6 203 2 215 126 0.0 6 125 1 216 223 0.0 6 221 1 1 217 152 0.0 6 148 4 218 142 0.0 6 139 3 219 139 0.0 6 138 1 220 162 0.0 6 161 1 221 131 0.0 6 128 3 222 137 0.0 6 136 1 223 109 0.0 6 106 3 224 146 0.0 6 141 4 0 1 225 152 0.0 6 150 2 226 218 0.0 6 215 3 227 225 0.0 6 223 2 228 174 0.0 6 173 1 229 163 0.0 6 162 1 230 114 0.0 6 112 1 1 231 144 0.0 6 143 1 232 151 0.0 6 151 233 120 0.0 6 119 1 234 123 0.0 6 123 235 115 0.0 6 113 2 236 115 0.0 6 113 2 237 207 0.0 6 204 2 1 238 141 0.0 6 140 1 239 156 0.0 6 155 1 240 271 0.0 6 268 3 241 149 0.0 6 145 4 242 181 0.0 6 175 6 243 168 0.0 6 165 3 244 223 0.0 6 217 6 245 142 0.0 6 141 1 246 220 0.0 6 213 7 247 199 0.0 6 194 5 248 234 0.0 6 234 249 120 0.0 6 120 250 172 0.0 6 167 4 1 251 171 0.0 6 164 7 252 347 0.0 6 341 6 253 151 0.0 6 149 2 254 166 0.0 6 164 2 255 183 0.0 6 176 7 256 214 0.0 6 212 2 257 296 0.0 6 290 6 258 204 0.0 6 198 6 259 416 0.0 6 410 6 260 202 0.0 6 195 7 261 396 0.0 6 393 2 1 262 158 0.0 6 148 10 263 310 0.0 6 306 4 264 173 0.0 6 170 3 265 175 0.0 6 169 6 266 195 0.0 6 190 5 267 285 0.0 6 284 1 268 176 0.0 6 174 2 269 265 0.0 6 260 5 270 220 0.0 6 219 1 271 234 0.0 6 228 6 272 197 0.0 6 194 3 273 170 0.0 6 166 4 274 183 0.0 6 181 1 1 275 313 0.0 6 307 5 1 276 462 0.0 6 449 12 1 277 605 0.0 6 591 14 278 1002 0.0 6 979 23 279 1226 0.0 6 1199 26 1 280 3233 0.0 6 3152 81 281 2567 0.0 6 2529 38 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 216 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 24 1 0.0 6 0 0 0 0 0 0 1 61 11 0.0 6 11 69 3 0.0 6 3 70 6 0.0 6 5 1 71 7 0.0 6 7 72 2 0.0 6 2 73 2 0.0 6 2 74 2 0.0 6 2 75 6 0.0 6 6 76 7 0.0 6 7 77 2 0.0 6 2 78 1 0.0 6 1 79 3 0.0 6 2 1 81 4 0.0 6 4 82 3 0.0 6 3 83 3 0.0 6 3 84 4 0.0 6 4 85 3 0.0 6 3 86 2 0.0 6 2 87 4 0.0 6 4 88 3 0.0 6 3 89 1 0.0 6 1 90 1 0.0 6 1 91 2 0.0 6 2 92 2 0.0 6 2 93 3 0.0 6 2 1 94 3 0.0 6 3 95 3 0.0 6 3 96 1 0.0 6 1 97 1 0.0 6 1 98 2 0.0 6 2 99 2 0.0 6 2 100 2 0.0 6 2 101 4 0.0 6 4 102 1 0.0 6 1 103 1 0.0 6 1 104 3 0.0 6 3 105 2 0.0 6 2 107 1 0.0 6 1 108 2 0.0 6 2 110 4 0.0 6 4 112 1 0.0 6 1 113 2 0.0 6 2 114 1 0.0 6 1 115 2 0.0 6 2 116 4 0.0 6 4 118 1 0.0 6 1 121 1 0.0 6 1 122 2 0.0 6 2 125 3 0.0 6 3 126 1 0.0 6 1 127 1 0.0 6 1 128 2 0.0 6 2 130 2 0.0 6 2 132 2 0.0 6 2 133 2 0.0 6 2 134 1 0.0 6 1 135 2 0.0 6 2 136 1 0.0 6 1 137 1 0.0 6 1 139 1 0.0 6 1 143 2 0.0 6 2 144 2 0.0 6 2 145 1 0.0 6 1 147 1 0.0 6 1 149 1 0.0 6 1 151 1 0.0 6 1 153 2 0.0 6 2 154 2 0.0 6 2 155 1 0.0 6 1 156 1 0.0 6 1 158 2 0.0 6 2 160 2 0.0 6 2 161 1 0.0 6 1 162 1 0.0 6 1 163 2 0.0 6 2 164 2 0.0 6 2 165 1 0.0 6 1 166 1 0.0 6 1 167 1 0.0 6 1 168 1 0.0 6 1 173 1 0.0 6 1 174 1 0.0 6 1 177 1 0.0 6 1 178 1 0.0 6 1 179 1 0.0 6 1 183 1 0.0 6 1 185 2 0.0 6 2 186 1 0.0 6 1 187 2 0.0 6 2 188 1 0.0 6 1 189 2 0.0 6 2 192 1 0.0 6 1 195 3 0.0 6 3 196 2 0.0 6 2 198 1 0.0 6 1 200 1 0.0 6 1 204 1 0.0 6 1 210 2 0.0 6 2 211 1 0.0 6 1 213 1 0.0 6 1 216 1 0.0 6 1 217 1 0.0 6 1 218 1 0.0 6 1 226 1 0.0 6 1 227 1 0.0 6 1 229 1 0.0 6 1 238 1 0.0 6 1 274 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 32290 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 31.8 1 1 9 2 0.1 2 1 1 12 2 0.0 3 2 13 5 0.0 3 3 1 0 1 14 3 0.0 4 3 15 1 0.0 4 0 1 16 3 0.0 4 3 17 6 0.0 5 6 18 3 0.0 5 3 19 2 0.0 5 0 2 20 23 0.0 6 22 1 21 20 0.0 6 20 22 10 0.0 6 10 23 6 0.0 6 6 24 2 0.0 6 2 25 2 0.0 6 2 26 4 0.0 6 4 27 5 0.0 6 5 28 3 0.0 6 3 29 2 0.0 6 2 30 8 0.0 6 8 31 4 0.0 6 4 32 2 0.0 6 2 33 2 0.0 6 2 34 3 0.0 6 3 35 5 0.0 6 5 36 2 0.0 6 2 37 7 0.0 6 7 38 4 0.0 6 4 39 5 0.0 6 5 40 2 0.0 6 2 41 3 0.0 6 3 42 3 0.0 6 1 2 43 2 0.0 6 0 2 44 6 0.0 6 6 45 7 0.0 6 7 46 4 0.0 6 4 47 2 0.0 6 2 48 8 0.0 6 8 49 8 0.0 6 8 50 4 0.0 6 4 51 6 0.0 6 5 1 52 12 0.0 6 12 53 3 0.0 6 3 54 7 0.0 6 7 55 7 0.0 6 6 1 56 4 0.0 6 3 0 0 0 1 57 4 0.0 6 4 58 3 0.0 6 3 59 8 0.0 6 7 1 60 10 0.0 6 10 61 3 0.0 6 3 62 4 0.0 6 2 1 0 1 63 5 0.0 6 5 64 8 0.0 6 8 65 5 0.0 6 5 66 9 0.0 6 8 0 0 0 1 67 7 0.0 6 6 1 68 17 0.0 6 17 69 67 0.0 6 65 2 70 16 0.0 6 15 1 71 29 0.0 6 29 72 18 0.0 6 17 1 73 18 0.0 6 18 74 6 0.0 6 6 75 9 0.0 6 9 76 10 0.0 6 10 77 13 0.0 6 13 78 14 0.0 6 14 79 9 0.0 6 9 80 11 0.0 6 11 81 9 0.0 6 9 82 8 0.0 6 8 83 22 0.0 6 20 2 84 20 0.0 6 19 1 85 24 0.0 6 24 86 14 0.0 6 14 87 13 0.0 6 13 88 18 0.0 6 18 89 15 0.0 6 15 90 9 0.0 6 8 0 1 91 17 0.0 6 17 92 16 0.0 6 15 1 93 26 0.0 6 25 1 94 13 0.0 6 13 95 27 0.0 6 26 1 96 28 0.0 6 25 2 1 97 25 0.0 6 24 0 0 1 98 19 0.0 6 19 99 20 0.0 6 19 1 100 22 0.0 6 22 101 14 0.0 6 14 102 26 0.0 6 25 1 103 24 0.0 6 21 3 104 25 0.0 6 25 105 30 0.0 6 28 1 0 0 1 106 31 0.0 6 30 0 1 107 24 0.0 6 24 108 29 0.0 6 28 1 109 24 0.0 6 24 110 19 0.0 6 18 1 111 25 0.0 6 24 1 112 31 0.0 6 27 4 113 25 0.0 6 25 114 35 0.0 6 34 1 115 34 0.0 6 34 116 40 0.0 6 40 117 32 0.0 6 32 118 45 0.0 6 43 2 119 52 0.0 6 52 120 54 0.0 6 52 2 121 48 0.0 6 46 1 1 122 42 0.0 6 42 123 36 0.0 6 34 1 1 124 47 0.0 6 47 125 66 0.0 6 65 0 0 0 0 1 126 58 0.0 6 57 1 127 64 0.0 6 61 3 128 59 0.0 6 57 2 129 58 0.0 6 56 2 130 57 0.0 6 55 2 131 92 0.0 6 90 2 132 89 0.0 6 88 1 133 106 0.0 6 102 3 0 0 1 134 115 0.0 6 110 5 135 163 0.0 6 160 3 136 176 0.0 6 175 1 137 191 0.0 6 189 2 138 258 0.0 6 258 139 340 0.0 6 339 1 140 344 0.0 6 342 2 141 330 0.0 6 326 4 142 326 0.0 6 326 143 407 0.0 6 404 3 144 409 0.0 6 408 1 145 322 0.0 6 322 146 294 0.0 6 292 2 147 245 0.0 6 244 1 148 313 0.0 6 311 2 149 355 0.0 6 352 2 0 0 1 150 342 0.0 6 339 3 151 284 0.0 6 282 2 152 464 0.0 6 458 6 153 298 0.0 6 297 1 154 375 0.0 6 370 5 155 381 0.0 6 376 5 156 305 0.0 6 302 3 157 357 0.0 6 354 3 158 336 0.0 6 335 1 159 250 0.0 6 246 4 160 301 0.0 6 300 1 161 271 0.0 6 269 2 162 236 0.0 6 233 3 163 245 0.0 6 243 2 164 233 0.0 6 232 1 165 258 0.0 6 256 1 1 166 284 0.0 6 283 1 167 246 0.0 6 241 5 168 302 0.0 6 295 6 0 1 169 203 0.0 6 202 1 170 271 0.0 6 270 1 171 324 0.0 6 316 8 172 195 0.0 6 189 5 1 173 240 0.0 6 237 3 174 337 0.0 6 329 8 175 412 0.0 6 403 7 0 1 1 176 307 0.0 6 304 2 1 177 349 0.0 6 344 4 0 0 0 0 1 178 302 0.0 6 299 2 1 179 344 0.0 6 341 3 180 283 0.0 6 278 5 181 267 0.0 6 261 5 1 182 121 0.0 6 119 2 183 234 0.0 6 232 2 184 121 0.0 6 119 2 185 188 0.0 6 183 4 0 1 186 538 0.0 6 526 12 187 411 0.0 6 403 7 0 1 188 387 0.0 6 379 7 1 189 393 0.0 6 380 13 190 496 0.0 6 491 5 191 458 0.0 6 454 3 1 192 343 0.0 6 332 10 0 0 1 193 390 0.0 6 387 3 194 278 0.0 6 269 9 195 339 0.0 6 331 7 1 196 586 0.0 6 576 10 197 198 0.0 6 191 7 198 552 0.0 6 544 8 199 527 0.0 6 513 14 200 311 0.0 6 306 5 201 388 0.0 6 380 8 202 313 0.0 6 308 5 203 255 0.0 6 252 3 204 328 0.0 6 320 8 205 201 0.0 6 199 2 206 226 0.0 6 224 2 207 203 0.0 6 195 6 0 1 1 208 313 0.0 6 305 8 209 96 0.0 6 93 3 210 187 0.0 6 181 6 211 297 0.0 6 289 8 212 228 0.0 6 222 6 213 65 0.0 6 62 2 0 1 214 254 0.0 6 250 4 215 383 0.0 6 381 1 1 216 357 0.0 6 341 16 217 238 0.0 6 236 2 218 172 0.0 6 164 8 219 147 0.0 6 145 2 220 132 0.0 6 131 1 221 189 0.0 6 185 4 222 204 0.0 6 198 6 223 251 0.0 6 247 3 0 0 1 224 149 0.0 6 146 3 225 164 0.0 6 163 1 226 153 0.0 6 151 2 227 228 0.0 6 222 6 228 143 0.0 6 139 3 1 229 217 0.0 6 212 5 230 571 0.0 6 559 12 231 173 0.0 6 162 10 1 232 271 0.0 6 269 2 233 120 0.0 6 116 4 234 187 0.0 6 181 6 235 86 0.0 6 84 2 236 96 0.0 6 94 2 237 90 0.0 6 87 3 238 54 0.0 6 54 239 27 0.0 6 25 2 240 76 0.0 6 74 2 241 49 0.0 6 49 242 60 0.0 6 58 2 243 46 0.0 6 43 2 0 0 1 244 46 0.0 6 43 3 245 68 0.0 6 67 1 246 48 0.0 6 44 4 247 35 0.0 6 34 1 248 32 0.0 6 31 1 249 48 0.0 6 45 3 250 22 0.0 6 22 251 56 0.0 6 56 252 32 0.0 6 30 2 253 46 0.0 6 45 1 254 9 0.0 6 9 255 19 0.0 6 19 256 6 0.0 6 6 279 3 0.0 6 3 280 10 0.0 6 10 281 17 0.0 6 17 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 219 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 68 1 0.0 6 1 69 1 0.0 6 1 70 2 0.0 6 2 71 8 0.0 6 8 72 10 0.0 6 10 73 3 0.0 6 3 74 3 0.0 6 3 75 3 0.0 6 3 76 8 0.0 6 8 77 3 0.0 6 3 78 6 0.0 6 6 79 5 0.0 6 5 80 6 0.0 6 6 81 5 0.0 6 5 82 3 0.0 6 3 83 4 0.0 6 4 84 2 0.0 6 2 85 3 0.0 6 3 86 4 0.0 6 4 87 9 0.0 6 9 88 1 0.0 6 1 89 2 0.0 6 2 90 3 0.0 6 3 91 1 0.0 6 1 92 3 0.0 6 3 93 3 0.0 6 3 94 3 0.0 6 3 95 3 0.0 6 3 96 2 0.0 6 2 97 2 0.0 6 2 98 1 0.0 6 1 99 3 0.0 6 3 101 3 0.0 6 3 103 6 0.0 6 6 105 1 0.0 6 1 107 1 0.0 6 1 108 2 0.0 6 2 109 3 0.0 6 3 111 1 0.0 6 1 112 2 0.0 6 2 113 1 0.0 6 1 114 1 0.0 6 1 115 3 0.0 6 3 118 1 0.0 6 1 119 3 0.0 6 3 120 1 0.0 6 1 121 1 0.0 6 1 122 2 0.0 6 2 123 2 0.0 6 2 124 3 0.0 6 3 126 3 0.0 6 3 127 1 0.0 6 1 129 1 0.0 6 1 130 3 0.0 6 3 131 1 0.0 6 1 132 3 0.0 6 3 134 1 0.0 6 1 135 1 0.0 6 1 136 2 0.0 6 2 137 2 0.0 6 2 140 2 0.0 6 2 141 1 0.0 6 1 142 2 0.0 6 2 145 1 0.0 6 1 146 1 0.0 6 1 148 1 0.0 6 1 149 2 0.0 6 2 150 3 0.0 6 3 151 2 0.0 6 2 152 3 0.0 6 3 153 2 0.0 6 2 156 1 0.0 6 1 157 1 0.0 6 1 159 1 0.0 6 1 160 2 0.0 6 2 163 1 0.0 6 1 167 1 0.0 6 1 168 1 0.0 6 1 169 2 0.0 6 2 172 1 0.0 6 1 174 1 0.0 6 1 175 4 0.0 6 3 0 0 0 1 177 1 0.0 6 1 180 1 0.0 6 1 181 1 0.0 6 1 183 2 0.0 6 2 184 1 0.0 6 1 187 1 0.0 6 1 190 1 0.0 6 1 194 1 0.0 6 1 196 1 0.0 6 1 201 1 0.0 6 1 210 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_07_171_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_07_398_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_07_171_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_07_398_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 7.78 s (78 µs/read; 0.77 M reads/minute). === Summary === Total read pairs processed: 99,504 Read 1 with adapter: 99,495 (100.0%) Read 2 with adapter: 99,436 (99.9%) == Read fate breakdown == Pairs that were too short: 62 (0.1%) Pairs discarded as untrimmed: 77 (0.1%) Pairs written (passing filters): 99,365 (99.9%) Total basepairs processed: 42,018,363 bp Read 1: 23,371,392 bp Read 2: 18,646,971 bp Total written (filtered): 6,486,001 bp (15.4%) Read 1: 3,242,312 bp Read 2: 3,243,689 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 96277 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 1554.8 0 2 2 4 4 388.7 1 1 3 5 1 97.2 1 0 1 6 6 24.3 1 1 0 5 7 3 6.1 2 3 9 1 0.4 2 1 12 1 0.0 3 1 14 1 0.0 4 1 15 1 0.0 4 1 16 3 0.0 4 3 17 9 0.0 5 9 19 5 0.0 5 2 3 20 153 0.0 6 149 3 1 21 66 0.0 6 64 2 22 62 0.0 6 59 3 23 38 0.0 6 37 1 24 35 0.0 6 34 1 25 26 0.0 6 24 0 1 1 26 32 0.0 6 30 2 27 20 0.0 6 19 1 28 13 0.0 6 13 29 8 0.0 6 6 1 0 1 30 10 0.0 6 10 31 6 0.0 6 6 32 12 0.0 6 12 33 7 0.0 6 7 34 10 0.0 6 9 0 0 0 0 1 35 9 0.0 6 8 1 36 13 0.0 6 12 1 37 15 0.0 6 14 0 1 38 8 0.0 6 6 2 39 14 0.0 6 13 1 40 22 0.0 6 22 41 9 0.0 6 9 42 10 0.0 6 10 43 17 0.0 6 14 1 0 0 1 1 44 11 0.0 6 10 1 45 9 0.0 6 8 1 46 9 0.0 6 8 1 47 9 0.0 6 9 48 6 0.0 6 6 49 5 0.0 6 4 1 50 8 0.0 6 8 51 13 0.0 6 12 1 52 12 0.0 6 12 53 6 0.0 6 6 54 8 0.0 6 7 1 55 9 0.0 6 9 56 3 0.0 6 3 57 6 0.0 6 6 58 5 0.0 6 5 59 6 0.0 6 5 1 60 9 0.0 6 9 61 5 0.0 6 5 62 12 0.0 6 12 63 7 0.0 6 7 64 10 0.0 6 10 65 9 0.0 6 9 66 11 0.0 6 9 2 67 8 0.0 6 8 68 10 0.0 6 9 1 69 7 0.0 6 7 70 9 0.0 6 8 1 71 12 0.0 6 12 72 10 0.0 6 8 1 1 73 11 0.0 6 11 74 17 0.0 6 17 75 12 0.0 6 12 76 13 0.0 6 13 77 13 0.0 6 13 78 11 0.0 6 9 1 0 0 0 0 1 79 19 0.0 6 19 80 13 0.0 6 13 81 9 0.0 6 8 0 0 0 0 1 82 15 0.0 6 13 1 1 83 19 0.0 6 19 84 15 0.0 6 15 85 24 0.0 6 24 86 23 0.0 6 23 87 13 0.0 6 12 1 88 15 0.0 6 15 89 12 0.0 6 11 0 0 1 90 14 0.0 6 14 91 15 0.0 6 14 1 92 7 0.0 6 7 93 14 0.0 6 14 94 14 0.0 6 13 1 95 20 0.0 6 20 96 9 0.0 6 9 97 14 0.0 6 14 98 12 0.0 6 12 99 16 0.0 6 15 0 0 0 0 0 1 100 20 0.0 6 19 1 101 20 0.0 6 19 1 102 13 0.0 6 13 103 24 0.0 6 20 3 0 0 0 0 1 104 22 0.0 6 20 2 105 20 0.0 6 20 106 24 0.0 6 21 3 107 12 0.0 6 12 108 20 0.0 6 20 109 20 0.0 6 19 0 0 0 0 1 110 21 0.0 6 20 1 111 34 0.0 6 33 1 112 31 0.0 6 31 113 38 0.0 6 35 2 0 0 0 0 1 114 29 0.0 6 28 1 115 38 0.0 6 38 116 31 0.0 6 29 2 117 32 0.0 6 29 3 118 43 0.0 6 43 119 40 0.0 6 39 1 120 48 0.0 6 45 2 0 1 121 42 0.0 6 41 0 0 0 0 1 122 27 0.0 6 27 123 44 0.0 6 43 1 124 38 0.0 6 36 2 125 44 0.0 6 44 126 44 0.0 6 43 0 0 0 0 0 1 127 30 0.0 6 30 128 34 0.0 6 34 129 55 0.0 6 54 1 130 37 0.0 6 37 131 60 0.0 6 59 1 132 41 0.0 6 40 1 133 45 0.0 6 45 134 56 0.0 6 52 3 0 0 0 0 1 135 41 0.0 6 39 2 136 58 0.0 6 58 137 56 0.0 6 55 1 138 70 0.0 6 70 139 66 0.0 6 66 140 72 0.0 6 72 141 102 0.0 6 101 0 1 142 100 0.0 6 99 1 143 268 0.0 6 268 144 313 0.0 6 311 2 145 591 0.0 6 588 3 146 392 0.0 6 391 1 147 377 0.0 6 377 148 595 0.0 6 595 149 505 0.0 6 505 150 467 0.0 6 464 2 1 151 574 0.0 6 574 152 462 0.0 6 457 5 153 582 0.0 6 580 2 154 529 0.0 6 528 1 155 543 0.0 6 542 1 156 493 0.0 6 493 157 397 0.0 6 397 158 374 0.0 6 372 1 0 1 159 413 0.0 6 408 5 160 392 0.0 6 389 3 161 455 0.0 6 450 4 0 0 0 0 1 162 623 0.0 6 620 3 163 555 0.0 6 553 1 0 0 0 0 1 164 515 0.0 6 511 3 0 0 1 165 635 0.0 6 632 3 166 485 0.0 6 477 8 167 613 0.0 6 612 0 1 168 512 0.0 6 509 3 169 450 0.0 6 449 1 170 551 0.0 6 551 171 533 0.0 6 527 5 0 0 0 1 172 637 0.0 6 635 1 0 0 0 0 1 173 528 0.0 6 522 6 174 469 0.0 6 463 5 0 1 175 571 0.0 6 568 3 176 469 0.0 6 468 1 177 530 0.0 6 527 3 178 498 0.0 6 494 3 0 0 1 179 445 0.0 6 440 5 180 551 0.0 6 549 1 0 0 1 181 1336 0.0 6 1328 6 0 0 1 1 182 630 0.0 6 628 1 0 1 183 534 0.0 6 529 4 0 0 0 0 1 184 583 0.0 6 582 0 1 185 644 0.0 6 642 2 186 786 0.0 6 779 5 0 1 0 0 1 187 875 0.0 6 869 6 188 678 0.0 6 675 2 0 1 189 617 0.0 6 617 190 626 0.0 6 623 3 191 703 0.0 6 698 5 192 1078 0.0 6 1069 8 1 193 936 0.0 6 929 7 194 742 0.0 6 736 5 0 0 0 1 195 920 0.0 6 916 2 1 1 196 1217 0.0 6 1210 5 0 2 197 1143 0.0 6 1135 8 198 1303 0.0 6 1295 4 2 1 0 1 199 1735 0.0 6 1728 7 200 1019 0.0 6 1010 8 0 0 0 1 201 967 0.0 6 961 4 0 1 1 202 1194 0.0 6 1187 5 1 0 0 0 1 203 957 0.0 6 954 3 204 1121 0.0 6 1112 8 0 0 0 1 205 1582 0.0 6 1576 6 206 1145 0.0 6 1138 4 2 0 0 0 1 207 1502 0.0 6 1495 6 0 0 1 208 1313 0.0 6 1304 5 0 3 0 0 1 209 1139 0.0 6 1129 7 1 0 0 2 210 1083 0.0 6 1077 5 1 211 1135 0.0 6 1126 7 1 0 0 0 1 212 1234 0.0 6 1228 6 213 1240 0.0 6 1228 10 1 0 0 0 1 214 1281 0.0 6 1274 6 0 0 1 215 1352 0.0 6 1346 5 0 0 0 0 1 216 1482 0.0 6 1476 4 2 217 1272 0.0 6 1267 4 0 1 218 1276 0.0 6 1272 4 219 1332 0.0 6 1327 4 1 220 1195 0.0 6 1185 9 0 1 221 985 0.0 6 980 3 2 222 979 0.0 6 974 5 223 1123 0.0 6 1118 4 0 0 1 224 1063 0.0 6 1057 6 225 1218 0.0 6 1212 6 226 1474 0.0 6 1470 3 1 227 1182 0.0 6 1178 4 228 994 0.0 6 986 6 1 0 0 1 229 972 0.0 6 969 3 230 793 0.0 6 788 4 1 231 942 0.0 6 939 2 0 0 0 0 1 232 1170 0.0 6 1167 3 233 893 0.0 6 889 4 234 761 0.0 6 753 6 0 0 1 1 235 717 0.0 6 709 6 2 236 651 0.0 6 642 8 0 1 237 745 0.0 6 739 4 0 0 1 0 1 238 643 0.0 6 641 2 239 625 0.0 6 624 0 0 1 240 599 0.0 6 595 4 241 750 0.0 6 747 2 1 242 531 0.0 6 531 243 542 0.0 6 537 3 0 1 0 1 244 578 0.0 6 576 2 245 502 0.0 6 498 4 246 396 0.0 6 392 4 247 389 0.0 6 389 248 316 0.0 6 315 0 0 1 249 338 0.0 6 334 1 0 1 0 0 2 250 342 0.0 6 341 1 251 307 0.0 6 304 2 0 0 0 0 1 252 270 0.0 6 269 0 1 253 285 0.0 6 282 3 254 294 0.0 6 292 1 1 255 256 0.0 6 256 256 216 0.0 6 215 1 257 217 0.0 6 216 1 258 251 0.0 6 250 1 259 219 0.0 6 219 260 231 0.0 6 229 1 0 0 1 261 204 0.0 6 203 0 0 0 0 0 1 262 170 0.0 6 169 1 263 162 0.0 6 162 264 157 0.0 6 155 1 0 1 265 193 0.0 6 191 1 1 266 138 0.0 6 138 267 140 0.0 6 140 268 161 0.0 6 160 1 269 143 0.0 6 141 2 270 127 0.0 6 126 1 271 101 0.0 6 99 2 272 104 0.0 6 104 273 84 0.0 6 84 274 102 0.0 6 102 275 115 0.0 6 115 276 176 0.0 6 173 2 0 1 277 202 0.0 6 198 4 278 280 0.0 6 276 4 279 930 0.0 6 920 9 0 0 0 0 1 280 1995 0.0 6 1984 9 0 0 0 2 281 6 0.0 6 6 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 3218 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 1 0.0 6 0 0 1 61 6 0.0 6 3 3 62 286 0.0 6 278 8 63 1 0.0 6 1 68 1 0.0 6 0 0 0 0 0 0 1 70 1 0.0 6 1 71 8 0.0 6 8 72 19 0.0 6 19 73 15 0.0 6 15 74 9 0.0 6 9 75 11 0.0 6 11 76 19 0.0 6 19 77 15 0.0 6 15 78 16 0.0 6 16 79 25 0.0 6 25 80 22 0.0 6 22 81 12 0.0 6 12 82 24 0.0 6 24 83 19 0.0 6 19 84 16 0.0 6 16 85 13 0.0 6 13 86 14 0.0 6 14 87 14 0.0 6 14 88 8 0.0 6 8 89 18 0.0 6 18 90 27 0.0 6 27 91 19 0.0 6 19 92 15 0.0 6 15 93 27 0.0 6 27 94 17 0.0 6 17 95 16 0.0 6 16 96 17 0.0 6 17 97 20 0.0 6 20 98 18 0.0 6 18 99 19 0.0 6 19 100 19 0.0 6 19 101 11 0.0 6 11 102 14 0.0 6 14 103 16 0.0 6 16 104 7 0.0 6 7 105 10 0.0 6 10 106 18 0.0 6 17 0 0 0 0 0 1 107 11 0.0 6 11 108 30 0.0 6 30 109 45 0.0 6 44 1 110 13 0.0 6 13 111 12 0.0 6 12 112 13 0.0 6 13 113 8 0.0 6 8 114 24 0.0 6 24 115 17 0.0 6 17 116 17 0.0 6 17 117 12 0.0 6 12 118 11 0.0 6 11 119 12 0.0 6 12 120 19 0.0 6 18 0 0 0 0 0 1 121 16 0.0 6 16 122 6 0.0 6 6 123 12 0.0 6 12 124 16 0.0 6 16 125 16 0.0 6 16 126 10 0.0 6 10 127 22 0.0 6 22 128 9 0.0 6 9 129 14 0.0 6 14 130 22 0.0 6 22 131 19 0.0 6 19 132 11 0.0 6 11 133 57 0.0 6 57 134 13 0.0 6 13 135 13 0.0 6 13 136 23 0.0 6 23 137 19 0.0 6 19 138 32 0.0 6 32 139 20 0.0 6 20 140 11 0.0 6 11 141 14 0.0 6 14 142 20 0.0 6 19 1 143 22 0.0 6 22 144 10 0.0 6 10 145 16 0.0 6 16 146 15 0.0 6 15 147 14 0.0 6 14 148 17 0.0 6 17 149 18 0.0 6 18 150 16 0.0 6 16 151 14 0.0 6 14 152 21 0.0 6 21 153 27 0.0 6 27 154 44 0.0 6 44 155 20 0.0 6 20 156 22 0.0 6 22 157 21 0.0 6 21 158 20 0.0 6 19 1 159 18 0.0 6 18 160 37 0.0 6 37 161 21 0.0 6 21 162 22 0.0 6 22 163 36 0.0 6 36 164 19 0.0 6 19 165 28 0.0 6 28 166 30 0.0 6 30 167 16 0.0 6 16 168 19 0.0 6 19 169 32 0.0 6 32 170 15 0.0 6 15 171 20 0.0 6 20 172 25 0.0 6 25 173 29 0.0 6 29 174 15 0.0 6 15 175 23 0.0 6 22 1 176 12 0.0 6 12 177 8 0.0 6 8 178 11 0.0 6 11 179 9 0.0 6 9 180 7 0.0 6 7 181 16 0.0 6 16 182 16 0.0 6 16 183 13 0.0 6 13 184 15 0.0 6 15 185 11 0.0 6 10 1 186 18 0.0 6 17 1 187 21 0.0 6 21 188 15 0.0 6 15 189 21 0.0 6 21 190 36 0.0 6 36 191 13 0.0 6 13 192 17 0.0 6 17 193 35 0.0 6 35 194 28 0.0 6 27 1 195 7 0.0 6 7 196 5 0.0 6 5 197 6 0.0 6 6 198 18 0.0 6 18 199 16 0.0 6 16 200 20 0.0 6 20 201 18 0.0 6 18 202 29 0.0 6 29 203 16 0.0 6 16 204 9 0.0 6 9 205 12 0.0 6 12 206 8 0.0 6 8 207 7 0.0 6 7 208 11 0.0 6 11 209 4 0.0 6 4 210 15 0.0 6 15 211 31 0.0 6 31 212 23 0.0 6 23 213 12 0.0 6 12 214 2 0.0 6 2 215 12 0.0 6 11 1 216 20 0.0 6 20 217 11 0.0 6 11 218 15 0.0 6 15 219 10 0.0 6 10 220 14 0.0 6 14 221 7 0.0 6 7 222 10 0.0 6 10 223 5 0.0 6 5 224 5 0.0 6 5 225 12 0.0 6 12 226 6 0.0 6 6 227 14 0.0 6 14 228 17 0.0 6 16 0 0 0 0 0 1 229 13 0.0 6 13 230 13 0.0 6 13 231 12 0.0 6 12 232 8 0.0 6 8 233 5 0.0 6 5 234 15 0.0 6 15 235 17 0.0 6 17 236 5 0.0 6 5 237 3 0.0 6 3 238 4 0.0 6 4 239 5 0.0 6 5 240 9 0.0 6 9 241 6 0.0 6 6 242 1 0.0 6 1 243 2 0.0 6 2 244 2 0.0 6 2 245 7 0.0 6 7 246 5 0.0 6 5 247 7 0.0 6 7 248 6 0.0 6 6 249 4 0.0 6 4 250 3 0.0 6 3 251 4 0.0 6 4 252 5 0.0 6 5 253 13 0.0 6 13 254 3 0.0 6 3 255 4 0.0 6 4 256 1 0.0 6 1 257 3 0.0 6 3 258 2 0.0 6 2 259 1 0.0 6 1 260 1 0.0 6 1 261 2 0.0 6 2 262 1 0.0 6 1 263 2 0.0 6 2 264 2 0.0 6 2 265 2 0.0 6 2 266 1 0.0 6 1 267 3 0.0 6 3 268 1 0.0 6 1 269 1 0.0 6 1 270 1 0.0 6 1 271 2 0.0 6 2 273 2 0.0 6 2 274 3 0.0 6 3 277 1 0.0 6 1 278 2 0.0 6 2 281 1 0.0 6 1 282 2 0.0 6 2 284 1 0.0 6 1 286 1 0.0 6 1 287 3 0.0 6 3 288 1 0.0 6 1 292 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 97005 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1554.8 0 0 1 7 1 6.1 2 0 1 8 1 1.5 2 0 0 1 10 4 0.1 3 3 1 11 8 0.0 3 8 12 2 0.0 3 1 1 13 12 0.0 3 11 1 14 9 0.0 4 9 15 2 0.0 4 1 1 16 14 0.0 4 10 4 17 36 0.0 5 29 6 1 18 21 0.0 5 19 2 19 25 0.0 5 20 5 20 117 0.0 6 117 21 75 0.0 6 72 3 22 43 0.0 6 42 1 23 25 0.0 6 24 1 24 41 0.0 6 37 3 1 25 40 0.0 6 38 2 26 25 0.0 6 25 27 29 0.0 6 26 3 28 34 0.0 6 33 0 0 1 29 22 0.0 6 20 1 0 0 1 30 21 0.0 6 19 2 31 13 0.0 6 12 1 32 19 0.0 6 17 1 0 1 33 24 0.0 6 22 1 0 0 1 34 24 0.0 6 23 1 35 17 0.0 6 17 36 18 0.0 6 18 37 18 0.0 6 17 1 38 18 0.0 6 16 2 39 18 0.0 6 16 2 40 13 0.0 6 12 1 41 15 0.0 6 13 2 42 15 0.0 6 12 2 1 43 20 0.0 6 18 2 44 20 0.0 6 17 3 45 15 0.0 6 14 1 46 25 0.0 6 24 1 47 17 0.0 6 16 1 48 31 0.0 6 28 2 1 49 24 0.0 6 22 1 1 50 31 0.0 6 27 2 1 0 0 0 1 51 32 0.0 6 27 5 52 26 0.0 6 23 2 1 53 42 0.0 6 35 6 1 54 34 0.0 6 29 4 0 0 1 55 26 0.0 6 25 0 0 0 0 0 1 56 32 0.0 6 28 4 57 31 0.0 6 29 2 58 40 0.0 6 37 1 2 59 38 0.0 6 32 6 60 29 0.0 6 25 3 1 61 36 0.0 6 32 4 62 33 0.0 6 29 2 0 1 0 0 1 63 43 0.0 6 39 3 1 64 49 0.0 6 42 6 0 1 65 51 0.0 6 48 3 66 74 0.0 6 69 4 1 67 221 0.0 6 209 8 2 1 0 0 1 68 66 0.0 6 58 6 1 0 1 69 97 0.0 6 90 7 70 46 0.0 6 44 2 71 66 0.0 6 65 1 72 38 0.0 6 35 2 0 1 73 57 0.0 6 52 5 74 52 0.0 6 47 5 75 66 0.0 6 61 5 76 51 0.0 6 47 2 1 0 0 1 77 82 0.0 6 75 7 78 68 0.0 6 60 5 3 79 94 0.0 6 86 8 80 70 0.0 6 66 3 1 81 85 0.0 6 79 2 2 0 1 1 82 66 0.0 6 62 4 83 100 0.0 6 96 2 0 0 0 1 1 84 74 0.0 6 60 13 0 1 85 68 0.0 6 60 5 1 0 0 2 86 49 0.0 6 47 1 1 87 89 0.0 6 85 4 88 83 0.0 6 79 3 1 89 81 0.0 6 77 3 0 0 0 1 90 75 0.0 6 70 3 0 0 1 0 1 91 86 0.0 6 80 5 0 0 1 92 95 0.0 6 89 4 2 93 126 0.0 6 117 7 1 1 94 100 0.0 6 94 5 0 0 0 1 95 128 0.0 6 117 8 2 0 1 96 115 0.0 6 111 4 97 115 0.0 6 111 3 0 0 0 1 98 124 0.0 6 123 1 99 88 0.0 6 84 2 0 1 1 100 103 0.0 6 98 2 0 0 2 1 101 143 0.0 6 137 6 102 127 0.0 6 120 7 103 174 0.0 6 167 6 0 0 1 104 309 0.0 6 296 10 2 1 105 153 0.0 6 148 4 1 106 154 0.0 6 144 9 0 0 1 107 169 0.0 6 166 2 0 0 0 1 108 144 0.0 6 135 7 0 0 1 0 1 109 165 0.0 6 158 2 3 2 110 199 0.0 6 190 9 111 199 0.0 6 197 2 112 241 0.0 6 232 8 0 1 113 184 0.0 6 178 6 114 206 0.0 6 202 4 115 261 0.0 6 251 9 1 116 237 0.0 6 233 4 117 237 0.0 6 230 6 0 0 1 118 267 0.0 6 261 5 0 0 0 1 119 232 0.0 6 221 11 120 220 0.0 6 213 7 121 242 0.0 6 234 6 0 1 0 0 1 122 303 0.0 6 294 9 123 310 0.0 6 304 4 1 0 0 0 1 124 364 0.0 6 356 4 0 0 2 0 2 125 380 0.0 6 373 5 2 126 400 0.0 6 388 10 1 0 1 127 454 0.0 6 440 10 2 2 128 456 0.0 6 441 12 1 1 1 129 509 0.0 6 496 13 130 690 0.0 6 666 20 2 1 1 131 605 0.0 6 591 11 0 0 0 1 2 132 611 0.0 6 595 13 0 2 1 133 646 0.0 6 626 16 0 1 2 0 1 134 788 0.0 6 760 24 0 2 1 0 1 135 716 0.0 6 699 14 2 0 1 136 700 0.0 6 688 12 137 658 0.0 6 644 13 0 1 138 720 0.0 6 703 13 1 2 0 1 139 938 0.0 6 921 15 0 0 0 1 1 140 1001 0.0 6 990 8 1 0 0 0 2 141 1220 0.0 6 1205 13 0 0 2 142 1277 0.0 6 1260 17 143 1109 0.0 6 1094 12 1 1 0 0 1 144 1422 0.0 6 1400 19 0 0 1 1 1 145 1445 0.0 6 1430 13 1 0 0 1 146 1567 0.0 6 1535 29 1 1 1 147 1617 0.0 6 1590 19 0 3 1 2 2 148 1850 0.0 6 1815 28 1 0 4 1 1 149 1944 0.0 6 1912 24 2 0 2 1 3 150 2388 0.0 6 2346 38 1 0 3 151 1954 0.0 6 1917 35 1 1 152 2183 0.0 6 2145 29 4 1 2 0 2 153 2092 0.0 6 2057 30 1 3 0 1 154 1719 0.0 6 1678 31 2 3 2 0 3 155 1785 0.0 6 1763 18 2 0 1 1 156 1615 0.0 6 1593 20 1 0 0 1 157 1949 0.0 6 1915 29 0 1 1 1 2 158 1898 0.0 6 1873 20 1 0 2 0 2 159 1669 0.0 6 1641 22 0 4 2 160 1698 0.0 6 1678 18 0 2 161 1639 0.0 6 1613 21 1 0 0 2 2 162 1427 0.0 6 1410 16 0 0 1 163 1471 0.0 6 1453 16 1 0 0 1 164 1300 0.0 6 1287 9 1 2 0 1 165 1343 0.0 6 1330 13 166 1672 0.0 6 1644 25 1 0 1 0 1 167 1367 0.0 6 1349 14 1 0 1 2 168 1615 0.0 6 1594 21 169 1338 0.0 6 1326 8 0 2 1 0 1 170 1341 0.0 6 1324 15 0 2 171 1450 0.0 6 1432 14 1 1 1 0 1 172 1414 0.0 6 1396 14 0 0 0 1 3 173 1323 0.0 6 1311 8 1 1 1 1 174 1308 0.0 6 1299 8 0 1 175 1354 0.0 6 1352 0 2 176 1405 0.0 6 1393 9 2 0 1 177 1190 0.0 6 1177 13 178 1111 0.0 6 1098 12 0 1 179 807 0.0 6 803 4 180 787 0.0 6 776 8 1 1 1 181 827 0.0 6 821 5 0 1 182 792 0.0 6 789 3 183 1144 0.0 6 1135 8 0 1 184 1354 0.0 6 1345 9 185 1145 0.0 6 1135 7 1 0 1 1 186 852 0.0 6 840 11 1 187 721 0.0 6 717 3 0 0 1 188 750 0.0 6 747 2 1 189 647 0.0 6 641 3 2 0 0 0 1 190 594 0.0 6 591 2 0 0 0 0 1 191 610 0.0 6 604 6 192 462 0.0 6 458 2 0 1 0 0 1 193 485 0.0 6 484 1 194 372 0.0 6 370 1 0 1 195 354 0.0 6 352 2 196 491 0.0 6 487 4 197 344 0.0 6 342 1 0 0 0 1 198 341 0.0 6 336 5 199 312 0.0 6 309 3 200 262 0.0 6 262 201 241 0.0 6 239 1 0 1 202 244 0.0 6 243 0 1 203 225 0.0 6 224 0 0 0 1 204 204 0.0 6 203 1 205 196 0.0 6 194 1 0 0 0 0 1 206 162 0.0 6 162 207 166 0.0 6 164 2 208 133 0.0 6 133 209 116 0.0 6 115 1 210 107 0.0 6 106 1 211 103 0.0 6 102 1 212 95 0.0 6 94 1 213 96 0.0 6 94 1 0 0 0 0 1 214 118 0.0 6 118 215 67 0.0 6 66 1 216 80 0.0 6 79 1 217 60 0.0 6 60 218 61 0.0 6 61 219 44 0.0 6 44 220 34 0.0 6 34 221 60 0.0 6 60 222 49 0.0 6 49 223 29 0.0 6 29 224 15 0.0 6 15 225 26 0.0 6 25 1 226 26 0.0 6 26 227 23 0.0 6 23 228 17 0.0 6 17 229 14 0.0 6 14 230 9 0.0 6 9 231 16 0.0 6 16 232 20 0.0 6 20 233 7 0.0 6 7 234 7 0.0 6 7 235 3 0.0 6 3 236 3 0.0 6 3 237 3 0.0 6 3 238 3 0.0 6 3 239 11 0.0 6 11 240 2 0.0 6 2 241 5 0.0 6 5 242 1 0.0 6 1 243 1 0.0 6 1 245 1 0.0 6 1 247 2 0.0 6 2 248 2 0.0 6 2 249 1 0.0 6 1 252 1 0.0 6 1 277 3 0.0 6 3 278 12 0.0 6 12 279 103 0.0 6 99 3 0 1 280 1 0.0 6 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2431 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 49 1 0.0 6 0 1 56 1 0.0 6 1 71 3 0.0 6 3 72 16 0.0 6 16 73 18 0.0 6 18 74 24 0.0 6 24 75 23 0.0 6 23 76 12 0.0 6 12 77 36 0.0 6 36 78 20 0.0 6 20 79 16 0.0 6 16 80 25 0.0 6 25 81 25 0.0 6 25 82 25 0.0 6 25 83 24 0.0 6 23 0 0 0 1 84 20 0.0 6 20 85 20 0.0 6 20 86 19 0.0 6 19 87 12 0.0 6 12 88 16 0.0 6 16 89 17 0.0 6 17 90 19 0.0 6 19 91 25 0.0 6 25 92 15 0.0 6 15 93 6 0.0 6 6 94 21 0.0 6 21 95 12 0.0 6 12 96 29 0.0 6 29 97 10 0.0 6 10 98 9 0.0 6 9 99 15 0.0 6 15 100 18 0.0 6 18 101 20 0.0 6 20 102 10 0.0 6 10 103 16 0.0 6 16 104 31 0.0 6 30 1 105 8 0.0 6 8 106 11 0.0 6 10 1 107 14 0.0 6 14 108 6 0.0 6 6 109 20 0.0 6 20 110 62 0.0 6 62 111 18 0.0 6 18 112 15 0.0 6 15 113 14 0.0 6 14 114 18 0.0 6 18 115 13 0.0 6 13 116 28 0.0 6 28 117 12 0.0 6 12 118 10 0.0 6 10 119 15 0.0 6 15 120 19 0.0 6 19 121 31 0.0 6 31 122 10 0.0 6 10 123 16 0.0 6 16 124 13 0.0 6 13 125 18 0.0 6 18 126 15 0.0 6 15 127 12 0.0 6 12 128 22 0.0 6 22 129 12 0.0 6 12 130 16 0.0 6 16 131 16 0.0 6 15 1 132 21 0.0 6 21 133 18 0.0 6 18 134 47 0.0 6 46 1 135 24 0.0 6 24 136 21 0.0 6 21 137 25 0.0 6 25 138 18 0.0 6 17 0 0 0 1 139 30 0.0 6 29 0 0 0 1 140 29 0.0 6 29 141 14 0.0 6 13 0 0 1 142 15 0.0 6 15 143 30 0.0 6 30 144 14 0.0 6 14 145 12 0.0 6 12 146 10 0.0 6 9 0 0 1 147 23 0.0 6 23 148 27 0.0 6 27 149 22 0.0 6 22 150 9 0.0 6 9 151 19 0.0 6 19 152 14 0.0 6 14 153 18 0.0 6 18 154 18 0.0 6 18 155 65 0.0 6 65 156 12 0.0 6 12 157 20 0.0 6 20 158 17 0.0 6 17 159 21 0.0 6 21 160 20 0.0 6 20 161 38 0.0 6 38 162 21 0.0 6 21 163 21 0.0 6 21 164 26 0.0 6 26 165 16 0.0 6 16 166 29 0.0 6 29 167 18 0.0 6 18 168 17 0.0 6 17 169 17 0.0 6 17 170 40 0.0 6 39 1 171 12 0.0 6 12 172 14 0.0 6 14 173 18 0.0 6 18 174 28 0.0 6 28 175 15 0.0 6 14 1 176 17 0.0 6 17 177 9 0.0 6 9 178 18 0.0 6 18 179 25 0.0 6 24 0 0 0 1 180 6 0.0 6 6 181 13 0.0 6 13 182 13 0.0 6 13 183 9 0.0 6 9 184 9 0.0 6 9 185 16 0.0 6 16 186 3 0.0 6 3 187 10 0.0 6 10 188 19 0.0 6 19 189 15 0.0 6 15 190 15 0.0 6 15 191 17 0.0 6 17 192 6 0.0 6 6 193 3 0.0 6 3 194 23 0.0 6 23 195 17 0.0 6 16 0 0 0 0 1 196 3 0.0 6 3 197 3 0.0 6 3 198 4 0.0 6 4 199 5 0.0 6 5 200 4 0.0 6 4 201 10 0.0 6 10 203 6 0.0 6 6 204 9 0.0 6 9 205 7 0.0 6 6 0 0 0 1 206 6 0.0 6 6 207 2 0.0 6 2 208 2 0.0 6 2 209 4 0.0 6 4 211 2 0.0 6 2 212 5 0.0 6 5 213 5 0.0 6 5 214 5 0.0 6 5 215 1 0.0 6 1 216 2 0.0 6 2 217 5 0.0 6 5 218 2 0.0 6 2 220 4 0.0 6 4 221 1 0.0 6 1 222 1 0.0 6 1 223 1 0.0 6 1 226 2 0.0 6 2 228 2 0.0 6 2 229 1 0.0 6 1 230 1 0.0 6 1 233 2 0.0 6 2 236 1 0.0 6 1 243 1 0.0 6 1 248 1 0.0 6 1 252 1 0.0 6 1 261 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_08_172_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_08_399_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_08_172_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_08_399_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 6.77 s (109 µs/read; 0.55 M reads/minute). === Summary === Total read pairs processed: 62,068 Read 1 with adapter: 62,060 (100.0%) Read 2 with adapter: 62,068 (100.0%) == Read fate breakdown == Pairs that were too short: 22 (0.0%) Pairs discarded as untrimmed: 8 (0.0%) Pairs written (passing filters): 62,038 (100.0%) Total basepairs processed: 27,255,148 bp Read 1: 15,281,637 bp Read 2: 11,973,511 bp Total written (filtered): 3,940,314 bp (14.5%) Read 1: 1,989,008 bp Read 2: 1,951,306 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 60720 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 969.8 0 3 1 6 2 15.2 1 2 7 2 3.8 2 1 0 1 9 1 0.2 2 0 1 10 1 0.1 3 0 1 16 2 0.0 4 2 17 4 0.0 5 4 19 3 0.0 5 3 20 75 0.0 6 72 3 21 21 0.0 6 21 22 28 0.0 6 28 23 16 0.0 6 16 24 10 0.0 6 10 25 20 0.0 6 20 26 12 0.0 6 12 27 5 0.0 6 5 28 13 0.0 6 13 29 8 0.0 6 8 30 6 0.0 6 6 31 7 0.0 6 7 32 1 0.0 6 1 33 6 0.0 6 6 34 4 0.0 6 2 2 35 4 0.0 6 4 36 3 0.0 6 3 37 5 0.0 6 4 1 38 7 0.0 6 7 39 3 0.0 6 3 40 8 0.0 6 8 41 3 0.0 6 3 42 6 0.0 6 6 43 2 0.0 6 2 44 5 0.0 6 5 45 3 0.0 6 3 46 1 0.0 6 1 47 4 0.0 6 3 1 48 4 0.0 6 3 0 1 49 7 0.0 6 7 50 5 0.0 6 5 51 5 0.0 6 5 52 8 0.0 6 8 53 6 0.0 6 6 55 3 0.0 6 2 1 56 3 0.0 6 3 57 1 0.0 6 1 58 4 0.0 6 3 1 59 4 0.0 6 4 60 2 0.0 6 2 61 3 0.0 6 3 62 6 0.0 6 6 63 2 0.0 6 2 64 3 0.0 6 2 1 65 3 0.0 6 3 66 9 0.0 6 8 0 1 67 6 0.0 6 5 1 68 3 0.0 6 3 69 5 0.0 6 4 1 70 6 0.0 6 5 1 71 7 0.0 6 6 1 72 5 0.0 6 5 73 4 0.0 6 4 74 8 0.0 6 8 75 10 0.0 6 9 1 76 7 0.0 6 6 1 77 8 0.0 6 8 78 3 0.0 6 3 79 4 0.0 6 4 80 5 0.0 6 4 1 81 7 0.0 6 6 0 1 82 6 0.0 6 6 83 11 0.0 6 11 84 6 0.0 6 6 85 10 0.0 6 10 86 6 0.0 6 6 87 14 0.0 6 14 88 5 0.0 6 5 89 7 0.0 6 7 90 8 0.0 6 6 2 91 3 0.0 6 3 92 5 0.0 6 5 93 6 0.0 6 6 94 7 0.0 6 7 95 9 0.0 6 9 96 10 0.0 6 10 97 11 0.0 6 11 98 9 0.0 6 9 99 11 0.0 6 11 100 11 0.0 6 10 1 101 14 0.0 6 14 102 10 0.0 6 10 103 7 0.0 6 6 1 104 13 0.0 6 13 105 12 0.0 6 11 1 106 11 0.0 6 11 107 16 0.0 6 15 1 108 22 0.0 6 21 1 109 9 0.0 6 9 110 17 0.0 6 17 111 20 0.0 6 20 112 10 0.0 6 10 113 14 0.0 6 13 1 114 20 0.0 6 20 115 18 0.0 6 18 116 23 0.0 6 21 2 117 18 0.0 6 17 1 118 19 0.0 6 18 0 0 0 0 1 119 17 0.0 6 15 1 0 0 0 1 120 19 0.0 6 19 121 18 0.0 6 15 3 122 18 0.0 6 18 123 22 0.0 6 22 124 16 0.0 6 15 1 125 20 0.0 6 19 0 1 126 24 0.0 6 23 1 127 17 0.0 6 17 128 28 0.0 6 27 1 129 35 0.0 6 34 1 130 32 0.0 6 32 131 20 0.0 6 19 1 132 33 0.0 6 32 1 133 22 0.0 6 22 134 21 0.0 6 21 135 44 0.0 6 42 2 136 37 0.0 6 37 137 42 0.0 6 42 138 42 0.0 6 41 1 139 50 0.0 6 50 140 44 0.0 6 42 2 141 61 0.0 6 61 142 141 0.0 6 139 2 143 272 0.0 6 270 2 144 296 0.0 6 295 1 145 195 0.0 6 192 3 146 233 0.0 6 233 147 346 0.0 6 345 1 148 244 0.0 6 244 149 287 0.0 6 285 2 150 334 0.0 6 333 1 151 222 0.0 6 219 3 152 274 0.0 6 272 2 153 296 0.0 6 295 0 1 154 213 0.0 6 213 155 176 0.0 6 176 156 234 0.0 6 234 157 186 0.0 6 185 0 0 0 0 1 158 175 0.0 6 175 159 193 0.0 6 190 2 1 160 206 0.0 6 205 1 161 202 0.0 6 202 162 217 0.0 6 215 2 163 289 0.0 6 287 2 164 268 0.0 6 267 0 1 165 376 0.0 6 373 3 166 224 0.0 6 224 167 250 0.0 6 249 1 168 243 0.0 6 241 2 169 246 0.0 6 244 2 170 253 0.0 6 251 2 171 276 0.0 6 275 0 0 0 0 0 1 172 301 0.0 6 296 4 1 173 361 0.0 6 360 1 174 282 0.0 6 282 175 207 0.0 6 205 2 176 249 0.0 6 246 3 177 250 0.0 6 246 4 178 225 0.0 6 222 3 179 261 0.0 6 261 180 640 0.0 6 638 2 181 334 0.0 6 334 182 244 0.0 6 244 183 297 0.0 6 294 2 0 1 184 263 0.0 6 263 185 361 0.0 6 358 3 186 385 0.0 6 380 4 1 187 364 0.0 6 363 1 188 346 0.0 6 345 1 189 352 0.0 6 350 1 1 190 394 0.0 6 392 2 191 426 0.0 6 422 3 0 0 0 0 1 192 442 0.0 6 440 1 0 1 193 440 0.0 6 437 2 1 194 401 0.0 6 396 4 1 195 553 0.0 6 550 2 1 196 547 0.0 6 542 5 197 609 0.0 6 606 3 198 811 0.0 6 805 3 0 2 0 0 1 199 623 0.0 6 617 5 0 0 0 1 200 559 0.0 6 550 8 1 201 529 0.0 6 526 3 202 523 0.0 6 522 1 203 539 0.0 6 533 5 1 204 741 0.0 6 737 4 205 555 0.0 6 549 6 206 627 0.0 6 621 4 1 0 0 0 1 207 817 0.0 6 811 6 208 547 0.0 6 543 3 0 0 0 0 1 209 600 0.0 6 599 1 210 609 0.0 6 605 4 211 607 0.0 6 603 4 212 638 0.0 6 635 3 213 674 0.0 6 668 6 214 697 0.0 6 690 6 0 1 215 722 0.0 6 716 5 0 0 0 1 216 911 0.0 6 904 7 217 650 0.0 6 647 3 218 725 0.0 6 725 219 693 0.0 6 690 2 1 220 633 0.0 6 629 2 0 1 0 1 221 572 0.0 6 569 3 222 645 0.0 6 642 3 223 564 0.0 6 559 4 1 224 577 0.0 6 569 8 225 688 0.0 6 683 5 226 675 0.0 6 669 6 227 687 0.0 6 685 1 1 228 567 0.0 6 566 1 229 479 0.0 6 478 1 230 488 0.0 6 483 3 2 231 526 0.0 6 521 4 0 0 0 0 1 232 518 0.0 6 514 3 0 0 0 1 233 494 0.0 6 487 6 1 234 553 0.0 6 546 7 235 468 0.0 6 462 5 0 1 236 407 0.0 6 406 1 237 518 0.0 6 510 8 238 425 0.0 6 424 1 239 434 0.0 6 428 6 240 461 0.0 6 459 2 241 412 0.0 6 406 6 242 458 0.0 6 455 3 243 427 0.0 6 421 4 2 244 415 0.0 6 405 10 245 299 0.0 6 297 2 246 317 0.0 6 314 3 247 270 0.0 6 265 5 248 324 0.0 6 317 6 0 0 0 1 249 301 0.0 6 297 3 1 250 280 0.0 6 278 2 251 274 0.0 6 268 6 252 237 0.0 6 234 3 253 186 0.0 6 185 1 254 232 0.0 6 231 0 1 255 183 0.0 6 181 2 256 256 0.0 6 252 4 257 214 0.0 6 209 5 258 204 0.0 6 202 1 1 259 191 0.0 6 189 1 0 0 0 1 260 302 0.0 6 296 6 261 195 0.0 6 192 3 262 152 0.0 6 150 2 263 169 0.0 6 166 3 264 162 0.0 6 161 1 265 145 0.0 6 143 2 266 208 0.0 6 203 5 267 125 0.0 6 123 2 268 170 0.0 6 166 4 269 222 0.0 6 221 1 270 188 0.0 6 188 271 175 0.0 6 171 4 272 122 0.0 6 118 4 273 200 0.0 6 195 5 274 326 0.0 6 320 6 275 263 0.0 6 259 4 276 339 0.0 6 330 9 277 437 0.0 6 423 14 278 766 0.0 6 747 18 0 1 279 3846 0.0 6 3766 78 1 0 0 1 280 2446 0.0 6 2411 34 1 281 18 0.0 6 15 2 0 1 282 3 0.0 6 3 284 1 0.0 6 1 286 1 0.0 6 1 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1340 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 57 4 0.0 6 0 0 0 0 4 62 12 0.0 6 12 66 1 0.0 6 1 71 2 0.0 6 2 72 12 0.0 6 12 73 9 0.0 6 9 74 7 0.0 6 7 75 3 0.0 6 3 76 9 0.0 6 9 77 8 0.0 6 8 78 10 0.0 6 10 79 6 0.0 6 6 80 10 0.0 6 10 81 7 0.0 6 7 82 7 0.0 6 7 83 11 0.0 6 11 84 10 0.0 6 10 85 5 0.0 6 5 86 11 0.0 6 11 87 3 0.0 6 3 88 5 0.0 6 5 89 7 0.0 6 7 90 6 0.0 6 6 91 13 0.0 6 13 92 15 0.0 6 15 93 15 0.0 6 15 94 18 0.0 6 18 95 5 0.0 6 5 96 9 0.0 6 9 97 8 0.0 6 8 98 4 0.0 6 4 99 10 0.0 6 10 100 8 0.0 6 8 101 11 0.0 6 11 102 3 0.0 6 3 103 8 0.0 6 8 104 1 0.0 6 1 105 5 0.0 6 5 106 7 0.0 6 7 107 4 0.0 6 4 108 9 0.0 6 9 109 21 0.0 6 21 110 4 0.0 6 4 111 2 0.0 6 2 112 8 0.0 6 8 113 4 0.0 6 4 114 5 0.0 6 5 115 3 0.0 6 3 116 6 0.0 6 6 117 4 0.0 6 4 118 5 0.0 6 5 119 10 0.0 6 10 120 12 0.0 6 11 1 121 5 0.0 6 5 122 7 0.0 6 7 123 5 0.0 6 5 124 6 0.0 6 6 125 11 0.0 6 11 126 5 0.0 6 5 127 6 0.0 6 6 128 5 0.0 6 5 130 8 0.0 6 8 131 5 0.0 6 5 132 10 0.0 6 10 133 20 0.0 6 20 134 8 0.0 6 8 135 10 0.0 6 10 136 12 0.0 6 12 137 6 0.0 6 6 138 9 0.0 6 9 139 13 0.0 6 13 140 6 0.0 6 6 141 9 0.0 6 9 142 10 0.0 6 10 143 7 0.0 6 7 144 13 0.0 6 13 145 10 0.0 6 10 146 10 0.0 6 10 147 11 0.0 6 11 148 11 0.0 6 11 149 7 0.0 6 7 150 7 0.0 6 7 151 13 0.0 6 13 152 8 0.0 6 8 153 6 0.0 6 6 154 9 0.0 6 9 155 6 0.0 6 6 156 12 0.0 6 12 157 9 0.0 6 9 158 8 0.0 6 7 1 159 11 0.0 6 11 160 9 0.0 6 9 161 10 0.0 6 10 162 15 0.0 6 15 163 6 0.0 6 6 164 6 0.0 6 6 165 14 0.0 6 14 166 12 0.0 6 12 167 7 0.0 6 7 168 7 0.0 6 7 169 18 0.0 6 18 170 9 0.0 6 9 171 9 0.0 6 9 172 9 0.0 6 9 173 18 0.0 6 17 1 174 10 0.0 6 10 175 6 0.0 6 6 176 7 0.0 6 7 177 5 0.0 6 5 178 16 0.0 6 16 179 10 0.0 6 10 180 4 0.0 6 4 181 6 0.0 6 6 182 10 0.0 6 10 183 5 0.0 6 5 184 14 0.0 6 14 185 5 0.0 6 5 186 7 0.0 6 7 187 9 0.0 6 9 188 8 0.0 6 8 189 7 0.0 6 6 0 0 0 0 0 1 190 9 0.0 6 9 191 7 0.0 6 7 192 8 0.0 6 8 193 4 0.0 6 4 194 4 0.0 6 4 195 4 0.0 6 4 196 12 0.0 6 12 197 9 0.0 6 9 198 7 0.0 6 7 199 10 0.0 6 10 200 9 0.0 6 9 201 5 0.0 6 5 202 10 0.0 6 10 203 4 0.0 6 4 204 7 0.0 6 7 205 8 0.0 6 8 206 9 0.0 6 9 207 6 0.0 6 6 208 4 0.0 6 4 209 2 0.0 6 2 210 1 0.0 6 1 211 9 0.0 6 9 212 3 0.0 6 3 213 9 0.0 6 9 214 2 0.0 6 2 215 4 0.0 6 4 216 6 0.0 6 6 217 4 0.0 6 4 218 4 0.0 6 4 219 3 0.0 6 3 220 3 0.0 6 3 221 5 0.0 6 5 222 2 0.0 6 2 223 6 0.0 6 6 224 6 0.0 6 6 225 6 0.0 6 6 226 6 0.0 6 6 227 8 0.0 6 8 228 1 0.0 6 1 229 2 0.0 6 2 230 1 0.0 6 1 231 4 0.0 6 4 232 11 0.0 6 11 233 9 0.0 6 9 234 4 0.0 6 4 235 8 0.0 6 8 236 4 0.0 6 4 237 3 0.0 6 3 238 3 0.0 6 3 239 5 0.0 6 5 240 3 0.0 6 3 241 2 0.0 6 2 243 1 0.0 6 1 244 1 0.0 6 1 245 1 0.0 6 1 246 1 0.0 6 1 247 2 0.0 6 2 248 1 0.0 6 1 250 4 0.0 6 4 251 3 0.0 6 3 252 2 0.0 6 2 253 1 0.0 6 1 254 2 0.0 6 2 255 2 0.0 6 2 257 2 0.0 6 2 258 1 0.0 6 1 259 1 0.0 6 1 260 1 0.0 6 1 261 1 0.0 6 1 262 1 0.0 6 1 265 2 0.0 6 2 266 2 0.0 6 2 267 1 0.0 6 1 272 1 0.0 6 1 273 1 0.0 6 1 282 1 0.0 6 1 283 1 0.0 6 1 287 1 0.0 6 1 303 1 0.0 6 1 305 1 0.0 6 1 306 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 60934 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 15.2 1 1 7 2 3.8 2 1 1 8 2 0.9 2 0 2 10 1 0.1 3 1 11 2 0.0 3 1 1 12 4 0.0 3 4 13 7 0.0 3 5 2 14 11 0.0 4 11 15 3 0.0 4 2 1 16 3 0.0 4 3 17 18 0.0 5 17 1 18 13 0.0 5 10 3 19 11 0.0 5 9 2 20 48 0.0 6 46 2 21 32 0.0 6 32 22 16 0.0 6 14 2 23 16 0.0 6 14 2 24 11 0.0 6 11 25 12 0.0 6 10 2 26 15 0.0 6 13 2 27 15 0.0 6 14 1 28 14 0.0 6 12 2 29 12 0.0 6 12 30 11 0.0 6 11 31 10 0.0 6 9 1 32 10 0.0 6 8 1 1 33 13 0.0 6 10 3 34 11 0.0 6 10 1 35 12 0.0 6 11 1 36 6 0.0 6 6 37 8 0.0 6 8 38 9 0.0 6 8 1 39 9 0.0 6 9 40 12 0.0 6 12 41 10 0.0 6 10 42 7 0.0 6 6 1 43 12 0.0 6 12 44 12 0.0 6 11 1 45 10 0.0 6 9 1 46 11 0.0 6 10 1 47 16 0.0 6 14 1 0 0 0 0 1 48 15 0.0 6 13 1 1 49 17 0.0 6 14 2 1 50 20 0.0 6 20 51 13 0.0 6 12 1 52 21 0.0 6 19 2 53 19 0.0 6 17 2 54 20 0.0 6 20 55 14 0.0 6 12 1 1 56 19 0.0 6 18 1 57 19 0.0 6 18 1 58 15 0.0 6 14 1 59 24 0.0 6 22 2 60 17 0.0 6 15 2 61 18 0.0 6 17 1 62 12 0.0 6 10 1 0 0 0 1 63 16 0.0 6 15 1 64 47 0.0 6 45 2 65 26 0.0 6 23 2 1 66 21 0.0 6 17 2 1 1 67 40 0.0 6 36 3 1 68 122 0.0 6 115 4 2 0 1 69 32 0.0 6 31 0 0 0 0 0 1 70 69 0.0 6 64 5 71 35 0.0 6 33 2 72 50 0.0 6 47 2 0 0 1 73 40 0.0 6 36 3 1 74 33 0.0 6 28 4 1 75 20 0.0 6 19 1 76 32 0.0 6 30 0 0 0 0 2 77 36 0.0 6 36 78 39 0.0 6 35 1 1 1 0 1 79 31 0.0 6 30 1 80 46 0.0 6 45 1 81 50 0.0 6 45 4 0 0 0 0 1 82 41 0.0 6 39 1 0 1 83 49 0.0 6 46 3 84 60 0.0 6 57 3 85 43 0.0 6 39 3 1 86 43 0.0 6 40 3 87 51 0.0 6 49 2 88 35 0.0 6 33 2 89 44 0.0 6 43 1 90 39 0.0 6 37 2 91 51 0.0 6 49 0 0 0 1 1 92 49 0.0 6 46 1 0 0 0 1 1 93 61 0.0 6 59 1 0 0 1 94 95 0.0 6 90 5 95 49 0.0 6 48 1 96 53 0.0 6 49 3 1 97 62 0.0 6 60 2 98 59 0.0 6 54 5 99 62 0.0 6 58 2 0 1 1 100 60 0.0 6 57 2 1 101 74 0.0 6 71 3 102 90 0.0 6 84 5 0 0 0 1 103 76 0.0 6 74 2 104 122 0.0 6 116 5 1 105 148 0.0 6 140 5 2 0 1 106 88 0.0 6 87 1 107 79 0.0 6 77 1 0 1 108 81 0.0 6 80 1 109 96 0.0 6 95 1 110 102 0.0 6 97 5 111 113 0.0 6 109 4 112 97 0.0 6 93 4 113 126 0.0 6 125 1 114 105 0.0 6 97 5 1 0 2 115 126 0.0 6 121 4 1 116 135 0.0 6 134 0 0 0 1 117 138 0.0 6 134 4 118 152 0.0 6 150 2 119 164 0.0 6 156 6 1 0 0 0 1 120 131 0.0 6 125 5 1 121 145 0.0 6 143 2 122 167 0.0 6 164 3 123 175 0.0 6 168 6 0 0 0 0 1 124 171 0.0 6 168 1 0 1 1 125 220 0.0 6 211 7 0 0 0 0 2 126 237 0.0 6 228 7 0 0 0 0 2 127 215 0.0 6 210 5 128 230 0.0 6 222 6 1 0 0 1 129 255 0.0 6 248 6 0 0 0 0 1 130 258 0.0 6 253 4 0 0 0 1 131 298 0.0 6 289 9 132 303 0.0 6 296 4 2 1 133 349 0.0 6 344 4 0 0 0 1 134 379 0.0 6 372 4 1 0 2 135 366 0.0 6 353 11 0 2 136 398 0.0 6 392 6 137 350 0.0 6 341 8 1 138 395 0.0 6 382 12 1 139 461 0.0 6 447 13 0 0 1 140 621 0.0 6 607 11 1 1 1 141 618 0.0 6 612 6 142 682 0.0 6 670 12 143 722 0.0 6 714 7 1 144 717 0.0 6 711 6 145 798 0.0 6 785 13 146 827 0.0 6 818 6 0 0 0 1 2 147 829 0.0 6 816 13 148 802 0.0 6 782 16 0 1 1 1 1 149 948 0.0 6 930 14 2 1 0 0 1 150 1079 0.0 6 1061 11 0 1 0 4 2 151 1243 0.0 6 1224 18 0 0 0 1 152 1028 0.0 6 1005 18 1 1 2 0 1 153 1139 0.0 6 1119 14 1 1 0 2 2 154 1120 0.0 6 1099 20 0 1 155 842 0.0 6 832 10 156 976 0.0 6 960 16 157 964 0.0 6 953 10 1 158 1001 0.0 6 991 8 0 0 2 159 950 0.0 6 938 12 160 900 0.0 6 889 11 161 916 0.0 6 910 6 162 812 0.0 6 800 10 0 0 0 1 1 163 754 0.0 6 743 10 0 1 164 795 0.0 6 783 10 0 2 165 790 0.0 6 783 6 0 0 0 0 1 166 730 0.0 6 718 10 0 0 0 2 167 830 0.0 6 821 9 168 820 0.0 6 805 13 2 169 836 0.0 6 826 9 0 1 170 709 0.0 6 705 3 0 1 171 794 0.0 6 786 7 0 1 172 754 0.0 6 750 3 0 0 0 0 1 173 801 0.0 6 787 11 1 0 1 0 1 174 857 0.0 6 846 9 1 0 0 1 175 861 0.0 6 849 11 1 176 764 0.0 6 756 7 0 0 1 177 888 0.0 6 877 8 2 0 0 1 178 633 0.0 6 623 10 179 703 0.0 6 695 8 180 538 0.0 6 529 8 0 0 1 181 575 0.0 6 558 17 182 453 0.0 6 450 3 183 551 0.0 6 541 9 1 184 578 0.0 6 575 3 185 755 0.0 6 748 7 186 862 0.0 6 850 11 0 0 1 187 604 0.0 6 595 8 0 1 188 569 0.0 6 563 6 189 579 0.0 6 577 1 1 190 701 0.0 6 692 8 1 191 602 0.0 6 587 15 192 517 0.0 6 505 11 1 193 356 0.0 6 352 4 194 407 0.0 6 400 6 0 0 1 195 454 0.0 6 445 9 196 710 0.0 6 692 18 197 486 0.0 6 471 13 1 0 1 198 591 0.0 6 575 15 0 0 1 199 615 0.0 6 598 16 0 1 200 369 0.0 6 362 7 201 420 0.0 6 413 6 0 0 0 1 202 246 0.0 6 237 8 1 203 236 0.0 6 234 2 204 248 0.0 6 245 3 205 184 0.0 6 183 1 206 129 0.0 6 128 1 207 121 0.0 6 120 1 208 165 0.0 6 162 3 209 134 0.0 6 131 3 210 157 0.0 6 155 2 211 138 0.0 6 137 1 212 138 0.0 6 136 2 213 104 0.0 6 101 2 0 1 214 132 0.0 6 126 6 215 281 0.0 6 272 8 1 216 163 0.0 6 160 2 1 217 171 0.0 6 164 7 218 119 0.0 6 116 3 219 141 0.0 6 134 7 220 83 0.0 6 81 2 221 80 0.0 6 75 3 1 0 1 222 129 0.0 6 124 4 1 223 157 0.0 6 155 2 224 117 0.0 6 116 1 225 117 0.0 6 114 3 226 95 0.0 6 93 2 227 53 0.0 6 53 228 71 0.0 6 71 229 72 0.0 6 70 2 230 61 0.0 6 59 2 231 56 0.0 6 53 3 232 93 0.0 6 90 3 233 82 0.0 6 82 234 15 0.0 6 15 235 24 0.0 6 22 2 236 14 0.0 6 13 1 237 12 0.0 6 12 238 13 0.0 6 13 239 19 0.0 6 19 240 27 0.0 6 27 241 32 0.0 6 31 1 242 54 0.0 6 52 2 243 29 0.0 6 28 1 244 15 0.0 6 15 245 8 0.0 6 8 246 15 0.0 6 15 247 9 0.0 6 9 248 17 0.0 6 17 249 11 0.0 6 11 250 1 0.0 6 1 251 3 0.0 6 3 252 2 0.0 6 2 253 9 0.0 6 9 254 2 0.0 6 2 257 2 0.0 6 2 279 12 0.0 6 12 280 42 0.0 6 42 281 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1134 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 6 49 15.2 1 0 0 49 53 1 0.0 6 1 55 1 0.0 6 1 67 5 0.0 6 5 70 1 0.0 6 1 71 5 0.0 6 5 72 10 0.0 6 10 73 11 0.0 6 11 74 4 0.0 6 4 75 10 0.0 6 10 76 14 0.0 6 14 77 8 0.0 6 8 78 7 0.0 6 7 79 6 0.0 6 6 80 10 0.0 6 10 81 9 0.0 6 9 82 11 0.0 6 11 83 5 0.0 6 5 84 3 0.0 6 3 85 13 0.0 6 13 86 11 0.0 6 11 87 9 0.0 6 9 88 7 0.0 6 7 89 2 0.0 6 2 90 5 0.0 6 5 91 7 0.0 6 7 92 7 0.0 6 7 93 3 0.0 6 3 94 14 0.0 6 14 95 4 0.0 6 4 96 11 0.0 6 11 97 8 0.0 6 8 98 4 0.0 6 4 99 14 0.0 6 14 100 10 0.0 6 10 101 16 0.0 6 16 102 6 0.0 6 5 0 0 0 1 103 5 0.0 6 4 1 104 6 0.0 6 6 105 8 0.0 6 8 106 8 0.0 6 8 107 2 0.0 6 2 108 6 0.0 6 6 109 28 0.0 6 28 110 9 0.0 6 9 111 2 0.0 6 2 112 6 0.0 6 5 1 113 8 0.0 6 8 114 5 0.0 6 5 115 11 0.0 6 11 116 9 0.0 6 9 117 8 0.0 6 8 118 5 0.0 6 5 119 10 0.0 6 10 120 8 0.0 6 8 121 8 0.0 6 8 122 4 0.0 6 4 123 7 0.0 6 7 124 11 0.0 6 11 125 5 0.0 6 5 126 11 0.0 6 11 127 14 0.0 6 14 128 2 0.0 6 2 129 2 0.0 6 2 130 8 0.0 6 8 131 2 0.0 6 2 132 14 0.0 6 14 133 16 0.0 6 16 134 14 0.0 6 14 135 12 0.0 6 12 136 13 0.0 6 13 137 4 0.0 6 4 138 9 0.0 6 9 139 6 0.0 6 6 140 7 0.0 6 7 141 8 0.0 6 8 142 9 0.0 6 9 143 8 0.0 6 8 144 5 0.0 6 5 145 9 0.0 6 9 146 5 0.0 6 5 147 10 0.0 6 10 148 5 0.0 6 5 149 5 0.0 6 5 150 8 0.0 6 8 151 5 0.0 6 5 152 10 0.0 6 10 153 9 0.0 6 8 1 154 11 0.0 6 11 155 7 0.0 6 6 0 0 0 1 156 15 0.0 6 15 157 12 0.0 6 12 158 11 0.0 6 10 0 0 0 1 159 7 0.0 6 7 160 6 0.0 6 6 161 12 0.0 6 12 162 11 0.0 6 11 163 12 0.0 6 12 164 8 0.0 6 8 165 13 0.0 6 13 166 11 0.0 6 11 167 5 0.0 6 5 168 11 0.0 6 10 1 169 11 0.0 6 11 170 2 0.0 6 2 171 5 0.0 6 5 172 9 0.0 6 9 173 28 0.0 6 28 174 5 0.0 6 5 175 6 0.0 6 6 176 10 0.0 6 10 177 10 0.0 6 10 178 12 0.0 6 12 179 3 0.0 6 3 180 5 0.0 6 4 0 0 0 1 181 5 0.0 6 5 182 6 0.0 6 6 183 9 0.0 6 9 184 9 0.0 6 9 185 11 0.0 6 11 186 10 0.0 6 10 187 11 0.0 6 11 188 8 0.0 6 8 189 5 0.0 6 5 191 4 0.0 6 4 192 4 0.0 6 4 193 7 0.0 6 7 194 1 0.0 6 1 196 9 0.0 6 9 197 8 0.0 6 8 198 4 0.0 6 4 200 4 0.0 6 4 201 2 0.0 6 2 203 2 0.0 6 2 204 2 0.0 6 2 205 3 0.0 6 3 206 2 0.0 6 2 207 1 0.0 6 1 209 2 0.0 6 2 210 1 0.0 6 1 212 1 0.0 6 1 213 1 0.0 6 1 214 2 0.0 6 2 215 2 0.0 6 2 217 2 0.0 6 2 219 2 0.0 6 2 222 1 0.0 6 1 224 1 0.0 6 1 227 1 0.0 6 1 235 1 0.0 6 1 238 1 0.0 6 1 239 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_09_173_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_09_400_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_09_173_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_09_400_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.88 s (69 µs/read; 0.87 M reads/minute). === Summary === Total read pairs processed: 71,094 Read 1 with adapter: 71,086 (100.0%) Read 2 with adapter: 71,069 (100.0%) == Read fate breakdown == Pairs that were too short: 19 (0.0%) Pairs discarded as untrimmed: 33 (0.0%) Pairs written (passing filters): 71,042 (99.9%) Total basepairs processed: 32,091,619 bp Read 1: 18,018,435 bp Read 2: 14,073,184 bp Total written (filtered): 4,337,508 bp (13.5%) Read 1: 2,251,501 bp Read 2: 2,086,007 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 70006 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1110.8 0 0 1 4 2 277.7 1 1 1 6 3 17.4 1 0 0 3 7 1 4.3 2 1 9 3 0.3 2 2 1 10 1 0.1 3 1 14 1 0.0 4 1 15 3 0.0 4 1 2 16 5 0.0 4 4 1 17 2 0.0 5 2 19 2 0.0 5 2 20 63 0.0 6 63 21 25 0.0 6 24 1 22 33 0.0 6 32 1 23 14 0.0 6 13 1 24 8 0.0 6 6 2 25 19 0.0 6 19 26 8 0.0 6 8 27 4 0.0 6 4 28 7 0.0 6 5 2 29 7 0.0 6 7 30 6 0.0 6 6 31 2 0.0 6 2 32 2 0.0 6 2 33 4 0.0 6 4 34 4 0.0 6 3 1 35 5 0.0 6 5 36 5 0.0 6 5 37 2 0.0 6 2 38 4 0.0 6 4 39 2 0.0 6 2 40 4 0.0 6 4 41 4 0.0 6 4 42 4 0.0 6 4 43 9 0.0 6 8 1 44 6 0.0 6 6 45 3 0.0 6 2 1 46 5 0.0 6 5 47 4 0.0 6 4 48 4 0.0 6 4 49 2 0.0 6 2 50 9 0.0 6 9 51 4 0.0 6 4 52 7 0.0 6 6 1 53 2 0.0 6 2 54 9 0.0 6 8 1 55 4 0.0 6 4 56 3 0.0 6 2 0 0 0 1 57 3 0.0 6 2 0 1 58 3 0.0 6 3 59 3 0.0 6 3 60 4 0.0 6 4 61 6 0.0 6 6 62 6 0.0 6 6 63 3 0.0 6 3 64 2 0.0 6 2 65 5 0.0 6 4 1 66 7 0.0 6 7 67 7 0.0 6 7 68 8 0.0 6 8 69 9 0.0 6 8 1 70 3 0.0 6 3 71 4 0.0 6 4 72 8 0.0 6 8 73 2 0.0 6 2 74 6 0.0 6 6 75 3 0.0 6 3 76 5 0.0 6 5 77 7 0.0 6 7 78 1 0.0 6 1 79 7 0.0 6 7 80 7 0.0 6 6 1 81 8 0.0 6 7 1 82 4 0.0 6 4 83 3 0.0 6 3 84 12 0.0 6 12 85 13 0.0 6 13 86 13 0.0 6 12 1 87 8 0.0 6 7 1 88 5 0.0 6 5 89 5 0.0 6 5 90 8 0.0 6 7 1 91 9 0.0 6 9 92 3 0.0 6 3 93 7 0.0 6 7 94 10 0.0 6 7 3 95 6 0.0 6 6 96 10 0.0 6 10 97 1 0.0 6 1 98 5 0.0 6 5 99 4 0.0 6 4 100 10 0.0 6 10 101 14 0.0 6 12 2 102 9 0.0 6 8 1 103 17 0.0 6 15 2 104 19 0.0 6 19 105 19 0.0 6 19 106 16 0.0 6 15 1 107 13 0.0 6 13 108 15 0.0 6 13 2 109 16 0.0 6 16 110 7 0.0 6 6 0 0 0 1 111 12 0.0 6 12 112 18 0.0 6 17 1 113 20 0.0 6 20 114 19 0.0 6 18 1 115 14 0.0 6 14 116 14 0.0 6 12 1 0 0 1 117 13 0.0 6 13 118 10 0.0 6 10 119 19 0.0 6 18 0 0 0 0 1 120 21 0.0 6 20 0 0 1 121 13 0.0 6 13 122 17 0.0 6 16 1 123 17 0.0 6 17 124 30 0.0 6 29 1 125 14 0.0 6 14 126 25 0.0 6 25 127 16 0.0 6 16 128 19 0.0 6 19 129 32 0.0 6 31 1 130 27 0.0 6 26 1 131 31 0.0 6 31 132 23 0.0 6 23 133 25 0.0 6 25 134 35 0.0 6 35 135 38 0.0 6 38 136 25 0.0 6 24 0 1 137 53 0.0 6 51 2 138 34 0.0 6 33 1 139 50 0.0 6 50 140 99 0.0 6 99 141 276 0.0 6 275 0 1 142 324 0.0 6 322 2 143 236 0.0 6 234 1 1 144 229 0.0 6 228 1 145 269 0.0 6 267 2 146 218 0.0 6 218 147 243 0.0 6 242 1 148 227 0.0 6 226 1 149 260 0.0 6 259 1 150 269 0.0 6 269 151 262 0.0 6 261 1 152 134 0.0 6 134 153 202 0.0 6 200 2 154 187 0.0 6 186 1 155 174 0.0 6 174 156 173 0.0 6 172 1 157 227 0.0 6 226 1 158 241 0.0 6 241 159 243 0.0 6 241 1 1 160 220 0.0 6 220 161 210 0.0 6 206 4 162 256 0.0 6 254 2 163 214 0.0 6 213 1 164 186 0.0 6 184 1 0 0 0 1 165 178 0.0 6 178 166 239 0.0 6 238 0 1 167 260 0.0 6 259 1 168 275 0.0 6 275 169 251 0.0 6 249 2 170 305 0.0 6 298 6 1 171 247 0.0 6 244 2 0 0 1 172 288 0.0 6 287 1 173 238 0.0 6 237 1 174 227 0.0 6 226 1 175 260 0.0 6 259 1 176 272 0.0 6 270 2 177 303 0.0 6 302 1 178 787 0.0 6 786 1 179 344 0.0 6 344 180 254 0.0 6 250 4 181 344 0.0 6 343 1 182 254 0.0 6 252 2 183 303 0.0 6 302 1 184 399 0.0 6 397 2 185 425 0.0 6 421 4 186 376 0.0 6 371 5 187 336 0.0 6 333 3 188 365 0.0 6 363 2 189 345 0.0 6 343 0 0 0 0 1 1 190 339 0.0 6 338 0 1 191 375 0.0 6 371 4 192 392 0.0 6 388 4 193 462 0.0 6 460 2 194 458 0.0 6 457 1 195 490 0.0 6 483 7 196 611 0.0 6 606 5 197 610 0.0 6 599 11 198 737 0.0 6 728 9 199 703 0.0 6 695 8 200 694 0.0 6 689 5 201 603 0.0 6 597 6 202 788 0.0 6 786 2 203 500 0.0 6 492 6 2 204 627 0.0 6 620 6 1 205 616 0.0 6 608 8 206 555 0.0 6 547 7 1 207 826 0.0 6 821 5 208 595 0.0 6 589 6 209 642 0.0 6 641 1 210 486 0.0 6 484 2 211 540 0.0 6 537 3 212 618 0.0 6 614 4 213 726 0.0 6 716 9 1 214 796 0.0 6 790 6 215 745 0.0 6 739 5 1 216 887 0.0 6 881 6 217 703 0.0 6 692 10 1 218 594 0.0 6 593 1 219 729 0.0 6 721 7 0 0 0 1 220 708 0.0 6 702 5 1 221 543 0.0 6 534 7 0 1 0 1 222 563 0.0 6 557 6 223 602 0.0 6 593 8 0 0 1 224 593 0.0 6 586 5 0 1 0 0 1 225 606 0.0 6 603 3 226 707 0.0 6 700 5 1 0 0 0 1 227 615 0.0 6 609 4 2 228 558 0.0 6 552 6 229 487 0.0 6 486 0 1 230 448 0.0 6 443 4 1 231 482 0.0 6 480 2 232 466 0.0 6 465 1 233 444 0.0 6 436 8 234 527 0.0 6 522 5 235 448 0.0 6 443 3 1 1 236 410 0.0 6 404 6 237 545 0.0 6 534 11 238 564 0.0 6 556 8 239 468 0.0 6 462 6 240 509 0.0 6 495 14 241 476 0.0 6 469 7 242 463 0.0 6 457 6 243 374 0.0 6 369 4 1 244 411 0.0 6 410 1 245 361 0.0 6 356 5 246 380 0.0 6 377 3 247 330 0.0 6 325 5 248 321 0.0 6 311 9 1 249 388 0.0 6 382 6 250 289 0.0 6 283 6 251 348 0.0 6 346 2 252 294 0.0 6 288 6 253 241 0.0 6 236 4 0 0 0 1 254 285 0.0 6 280 5 255 310 0.0 6 306 4 256 321 0.0 6 310 11 257 284 0.0 6 276 7 1 258 361 0.0 6 355 6 259 430 0.0 6 422 8 260 417 0.0 6 410 7 261 267 0.0 6 264 3 262 201 0.0 6 200 1 263 305 0.0 6 295 8 2 264 237 0.0 6 232 5 265 217 0.0 6 212 5 266 250 0.0 6 244 6 267 344 0.0 6 335 9 268 269 0.0 6 266 2 1 269 431 0.0 6 423 8 270 238 0.0 6 227 10 1 271 292 0.0 6 287 5 272 298 0.0 6 289 7 1 1 273 347 0.0 6 343 4 274 468 0.0 6 454 14 275 324 0.0 6 312 11 0 1 276 607 0.0 6 589 17 1 277 1039 0.0 6 1012 24 3 278 1638 0.0 6 1587 51 279 6152 0.0 6 6009 137 5 1 280 4344 0.0 6 4229 112 3 281 30 0.0 6 26 4 282 1 0.0 6 1 284 1 0.0 6 1 285 1 0.0 6 1 286 4 0.0 6 4 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1080 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 1 0.0 6 1 62 18 0.0 6 18 71 7 0.0 6 7 72 11 0.0 6 10 1 73 9 0.0 6 9 74 4 0.0 6 4 75 10 0.0 6 10 76 7 0.0 6 7 77 4 0.0 6 4 78 8 0.0 6 8 79 3 0.0 6 3 80 5 0.0 6 5 81 4 0.0 6 4 82 4 0.0 6 4 83 1 0.0 6 1 84 9 0.0 6 9 85 3 0.0 6 3 86 3 0.0 6 3 87 4 0.0 6 4 88 3 0.0 6 3 89 4 0.0 6 4 90 8 0.0 6 8 91 5 0.0 6 5 92 6 0.0 6 6 93 8 0.0 6 8 94 4 0.0 6 4 95 5 0.0 6 5 96 5 0.0 6 5 97 3 0.0 6 3 98 6 0.0 6 6 99 4 0.0 6 4 100 5 0.0 6 5 101 7 0.0 6 7 102 5 0.0 6 5 103 4 0.0 6 4 104 7 0.0 6 7 105 2 0.0 6 2 106 1 0.0 6 1 107 7 0.0 6 7 108 12 0.0 6 12 109 22 0.0 6 22 110 5 0.0 6 5 111 9 0.0 6 9 112 5 0.0 6 4 1 113 4 0.0 6 4 114 8 0.0 6 8 115 7 0.0 6 7 116 5 0.0 6 5 117 4 0.0 6 4 118 8 0.0 6 8 119 8 0.0 6 8 120 6 0.0 6 6 121 7 0.0 6 7 122 10 0.0 6 10 123 1 0.0 6 1 124 7 0.0 6 7 125 3 0.0 6 3 126 4 0.0 6 4 127 4 0.0 6 4 128 5 0.0 6 5 129 3 0.0 6 3 130 6 0.0 6 6 131 6 0.0 6 6 132 8 0.0 6 8 133 5 0.0 6 5 134 7 0.0 6 7 135 9 0.0 6 9 136 3 0.0 6 3 137 6 0.0 6 6 138 11 0.0 6 11 139 6 0.0 6 6 140 3 0.0 6 3 141 7 0.0 6 7 142 8 0.0 6 8 143 8 0.0 6 8 144 8 0.0 6 8 145 3 0.0 6 3 146 5 0.0 6 5 147 6 0.0 6 6 148 10 0.0 6 10 149 4 0.0 6 4 150 5 0.0 6 5 151 6 0.0 6 6 152 6 0.0 6 6 153 4 0.0 6 4 154 6 0.0 6 6 155 6 0.0 6 6 156 14 0.0 6 14 157 7 0.0 6 7 158 12 0.0 6 12 159 9 0.0 6 9 160 11 0.0 6 11 161 8 0.0 6 8 162 12 0.0 6 12 163 9 0.0 6 9 164 5 0.0 6 5 165 6 0.0 6 6 166 8 0.0 6 8 167 10 0.0 6 10 168 7 0.0 6 7 169 13 0.0 6 13 170 13 0.0 6 13 171 14 0.0 6 14 172 8 0.0 6 8 173 9 0.0 6 9 174 7 0.0 6 7 175 9 0.0 6 9 176 4 0.0 6 4 177 8 0.0 6 8 178 5 0.0 6 5 179 8 0.0 6 8 180 9 0.0 6 9 181 4 0.0 6 4 182 6 0.0 6 6 183 4 0.0 6 4 184 7 0.0 6 7 185 5 0.0 6 5 186 3 0.0 6 3 187 14 0.0 6 14 188 8 0.0 6 8 189 11 0.0 6 11 190 6 0.0 6 6 191 5 0.0 6 5 192 11 0.0 6 11 193 8 0.0 6 8 194 8 0.0 6 8 195 5 0.0 6 5 196 2 0.0 6 2 197 5 0.0 6 5 198 6 0.0 6 6 199 7 0.0 6 7 200 7 0.0 6 7 201 8 0.0 6 8 202 7 0.0 6 7 203 7 0.0 6 7 204 1 0.0 6 1 205 1 0.0 6 1 206 6 0.0 6 6 207 1 0.0 6 1 208 6 0.0 6 6 209 3 0.0 6 3 210 5 0.0 6 5 211 5 0.0 6 5 212 1 0.0 6 1 213 5 0.0 6 5 214 3 0.0 6 3 215 3 0.0 6 3 216 8 0.0 6 8 217 5 0.0 6 5 218 3 0.0 6 3 219 5 0.0 6 5 220 9 0.0 6 9 221 9 0.0 6 9 222 2 0.0 6 2 223 1 0.0 6 1 224 6 0.0 6 6 225 6 0.0 6 6 226 3 0.0 6 3 227 4 0.0 6 4 228 2 0.0 6 2 230 2 0.0 6 2 231 8 0.0 6 8 232 4 0.0 6 4 233 3 0.0 6 3 234 3 0.0 6 3 235 1 0.0 6 1 236 2 0.0 6 2 237 2 0.0 6 2 238 4 0.0 6 4 239 2 0.0 6 2 241 1 0.0 6 1 242 2 0.0 6 2 243 1 0.0 6 1 244 1 0.0 6 1 245 5 0.0 6 5 246 1 0.0 6 1 247 4 0.0 6 4 248 1 0.0 6 1 249 1 0.0 6 1 250 1 0.0 6 1 252 3 0.0 6 3 253 1 0.0 6 1 255 1 0.0 6 1 256 2 0.0 6 2 257 1 0.0 6 1 258 2 0.0 6 2 259 2 0.0 6 2 260 2 0.0 6 2 263 1 0.0 6 1 267 1 0.0 6 1 268 3 0.0 6 3 270 2 0.0 6 2 271 2 0.0 6 2 281 1 0.0 6 1 293 1 0.0 6 1 298 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 70163 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 4.3 2 1 11 2 0.0 3 1 1 12 2 0.0 3 2 13 7 0.0 3 6 0 1 14 9 0.0 4 9 15 4 0.0 4 4 16 2 0.0 4 2 17 13 0.0 5 10 2 0 1 18 1 0.0 5 1 19 41 0.0 5 36 5 20 67 0.0 6 66 1 21 43 0.0 6 41 1 1 22 24 0.0 6 24 23 14 0.0 6 14 24 18 0.0 6 16 2 25 13 0.0 6 13 26 10 0.0 6 10 27 10 0.0 6 10 28 11 0.0 6 11 29 14 0.0 6 13 1 30 13 0.0 6 11 2 31 12 0.0 6 10 2 32 10 0.0 6 10 33 13 0.0 6 10 2 0 1 34 9 0.0 6 9 35 6 0.0 6 6 36 5 0.0 6 4 1 37 12 0.0 6 9 2 1 38 7 0.0 6 7 39 8 0.0 6 7 1 40 9 0.0 6 9 41 11 0.0 6 10 1 42 9 0.0 6 8 1 43 8 0.0 6 8 44 16 0.0 6 15 0 0 0 0 1 45 13 0.0 6 12 1 46 14 0.0 6 14 47 9 0.0 6 8 1 48 11 0.0 6 10 0 0 1 49 23 0.0 6 23 50 26 0.0 6 25 0 0 0 1 51 18 0.0 6 16 1 1 52 20 0.0 6 19 1 53 15 0.0 6 14 1 54 12 0.0 6 11 1 55 28 0.0 6 24 4 56 19 0.0 6 16 1 1 0 0 1 57 22 0.0 6 21 1 58 23 0.0 6 20 2 1 59 29 0.0 6 26 3 60 14 0.0 6 12 2 61 14 0.0 6 12 0 0 0 0 2 62 19 0.0 6 18 0 0 0 1 63 27 0.0 6 25 1 1 64 18 0.0 6 15 1 1 0 0 1 65 25 0.0 6 22 3 66 27 0.0 6 25 2 67 25 0.0 6 24 1 68 25 0.0 6 23 2 69 34 0.0 6 30 2 2 70 144 0.0 6 134 6 1 2 0 1 71 38 0.0 6 36 0 0 0 2 72 72 0.0 6 66 4 1 0 0 1 73 26 0.0 6 24 1 0 0 0 0 1 74 33 0.0 6 30 1 0 0 0 0 2 75 34 0.0 6 33 0 1 76 33 0.0 6 31 2 77 30 0.0 6 28 0 0 1 1 78 35 0.0 6 32 3 79 28 0.0 6 28 80 43 0.0 6 40 1 1 0 0 0 1 81 35 0.0 6 33 2 82 38 0.0 6 35 2 0 1 83 43 0.0 6 39 4 84 51 0.0 6 44 3 2 0 0 0 2 85 57 0.0 6 56 1 86 55 0.0 6 54 1 87 45 0.0 6 42 2 1 88 30 0.0 6 30 89 36 0.0 6 33 3 90 44 0.0 6 42 2 91 37 0.0 6 36 1 92 45 0.0 6 42 3 93 67 0.0 6 65 2 94 59 0.0 6 56 2 0 0 1 95 51 0.0 6 48 2 1 96 79 0.0 6 74 4 0 0 0 1 97 53 0.0 6 50 2 0 0 1 98 44 0.0 6 42 2 99 92 0.0 6 87 4 0 0 0 0 1 100 79 0.0 6 73 5 1 101 61 0.0 6 58 3 102 77 0.0 6 71 5 0 0 1 103 87 0.0 6 83 3 0 0 1 104 98 0.0 6 93 4 0 0 0 0 1 105 98 0.0 6 95 3 106 88 0.0 6 85 2 0 0 0 0 1 107 145 0.0 6 142 2 0 0 0 0 1 108 107 0.0 6 104 3 109 86 0.0 6 81 4 0 0 0 1 110 86 0.0 6 82 3 0 0 0 1 111 94 0.0 6 91 3 112 89 0.0 6 85 4 113 119 0.0 6 115 3 1 114 116 0.0 6 113 3 115 120 0.0 6 115 3 1 0 0 1 116 110 0.0 6 108 2 117 92 0.0 6 91 0 0 1 118 148 0.0 6 145 3 119 142 0.0 6 138 4 120 157 0.0 6 150 5 1 0 1 121 162 0.0 6 155 7 122 132 0.0 6 130 1 1 123 147 0.0 6 144 3 124 137 0.0 6 132 5 125 163 0.0 6 157 5 0 1 126 199 0.0 6 190 7 1 0 0 1 127 205 0.0 6 200 3 0 1 0 0 1 128 225 0.0 6 218 6 0 0 1 129 203 0.0 6 198 4 0 0 0 0 1 130 214 0.0 6 210 4 131 284 0.0 6 280 4 132 278 0.0 6 273 4 0 1 133 303 0.0 6 297 4 0 0 2 134 354 0.0 6 346 8 135 439 0.0 6 427 11 0 0 0 0 1 136 397 0.0 6 391 6 137 422 0.0 6 413 6 2 0 0 1 138 417 0.0 6 406 9 0 1 0 0 1 139 442 0.0 6 432 9 1 140 535 0.0 6 525 6 0 2 1 0 1 141 614 0.0 6 608 6 142 603 0.0 6 589 8 1 0 2 1 2 143 593 0.0 6 585 7 0 0 0 1 144 726 0.0 6 705 18 2 0 0 1 145 647 0.0 6 634 12 1 146 650 0.0 6 644 4 1 0 0 1 147 697 0.0 6 690 6 1 148 721 0.0 6 708 11 0 0 2 149 737 0.0 6 725 11 0 1 150 850 0.0 6 841 8 0 0 0 0 1 151 816 0.0 6 802 10 2 0 2 152 1050 0.0 6 1027 20 2 1 153 1205 0.0 6 1181 20 1 2 0 1 154 916 0.0 6 903 9 0 1 1 2 155 1057 0.0 6 1043 13 0 0 1 156 1120 0.0 6 1105 12 0 0 0 2 1 157 979 0.0 6 963 14 0 0 2 158 989 0.0 6 969 16 1 1 1 0 1 159 846 0.0 6 834 10 0 0 1 0 1 160 1064 0.0 6 1048 15 1 161 959 0.0 6 949 9 1 162 800 0.0 6 788 12 163 852 0.0 6 839 12 1 164 782 0.0 6 773 7 0 0 1 0 1 165 804 0.0 6 792 10 1 1 166 852 0.0 6 842 9 1 167 775 0.0 6 767 7 0 0 1 168 789 0.0 6 778 10 0 0 0 1 169 807 0.0 6 796 11 170 648 0.0 6 644 3 0 1 171 934 0.0 6 921 13 172 650 0.0 6 646 4 173 769 0.0 6 759 9 0 1 174 871 0.0 6 857 14 175 1090 0.0 6 1075 12 3 176 929 0.0 6 913 16 177 951 0.0 6 937 10 0 2 1 1 178 710 0.0 6 700 9 0 1 179 761 0.0 6 750 10 0 0 0 0 1 180 693 0.0 6 680 12 0 0 0 0 1 181 750 0.0 6 739 10 1 182 548 0.0 6 533 14 0 0 1 183 719 0.0 6 711 7 0 0 1 184 415 0.0 6 406 8 1 185 427 0.0 6 423 4 186 882 0.0 6 869 10 1 1 0 1 187 1005 0.0 6 974 28 0 2 1 188 1065 0.0 6 1042 22 1 189 767 0.0 6 748 18 0 1 190 1017 0.0 6 996 18 1 1 0 1 191 880 0.0 6 859 20 0 0 1 192 775 0.0 6 763 10 0 0 1 0 1 193 570 0.0 6 558 11 1 194 715 0.0 6 694 20 1 195 707 0.0 6 685 20 1 0 1 196 1172 0.0 6 1151 21 197 588 0.0 6 571 17 198 1212 0.0 6 1185 27 199 1515 0.0 6 1481 30 2 0 2 200 710 0.0 6 687 22 0 1 201 791 0.0 6 776 14 0 0 0 1 202 517 0.0 6 500 17 203 420 0.0 6 411 9 204 424 0.0 6 409 11 0 0 3 1 205 260 0.0 6 252 8 206 252 0.0 6 247 4 1 207 227 0.0 6 223 3 0 0 0 0 1 208 292 0.0 6 285 7 209 249 0.0 6 243 5 0 0 0 0 1 210 236 0.0 6 230 6 211 352 0.0 6 343 9 212 275 0.0 6 270 3 1 0 1 213 132 0.0 6 128 3 1 214 205 0.0 6 200 4 1 215 221 0.0 6 217 4 216 348 0.0 6 336 12 217 464 0.0 6 447 16 0 0 1 218 279 0.0 6 270 9 219 290 0.0 6 282 7 1 220 248 0.0 6 238 9 0 1 221 300 0.0 6 290 9 0 0 1 222 249 0.0 6 240 8 1 223 217 0.0 6 211 4 0 1 1 224 244 0.0 6 228 15 0 0 1 225 303 0.0 6 289 14 226 260 0.0 6 253 7 227 199 0.0 6 191 8 228 132 0.0 6 127 5 229 184 0.0 6 180 4 230 122 0.0 6 122 231 138 0.0 6 137 1 232 198 0.0 6 192 5 0 0 1 233 134 0.0 6 129 5 234 69 0.0 6 68 1 235 54 0.0 6 53 1 236 26 0.0 6 26 237 49 0.0 6 48 1 238 20 0.0 6 19 1 239 27 0.0 6 27 240 42 0.0 6 39 3 241 86 0.0 6 83 3 242 75 0.0 6 74 1 243 40 0.0 6 39 1 244 114 0.0 6 111 3 245 58 0.0 6 58 246 52 0.0 6 52 247 44 0.0 6 42 2 248 22 0.0 6 22 249 25 0.0 6 24 1 250 18 0.0 6 18 251 34 0.0 6 33 1 252 9 0.0 6 9 253 4 0.0 6 4 254 19 0.0 6 19 255 3 0.0 6 3 256 4 0.0 6 4 257 1 0.0 6 1 258 1 0.0 6 1 265 1 0.0 6 1 277 1 0.0 6 1 278 2 0.0 6 2 279 1 0.0 6 1 280 1 0.0 6 1 281 7 0.0 6 7 282 49 0.0 6 48 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 906 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 45 1 0.0 6 0 1 66 1 0.0 6 1 68 1 0.0 6 1 70 10 0.0 6 9 1 71 8 0.0 6 8 72 6 0.0 6 6 73 5 0.0 6 5 74 9 0.0 6 9 75 9 0.0 6 9 76 4 0.0 6 4 77 10 0.0 6 10 78 8 0.0 6 8 79 5 0.0 6 5 80 6 0.0 6 6 81 6 0.0 6 6 82 4 0.0 6 4 83 10 0.0 6 10 84 3 0.0 6 3 85 2 0.0 6 2 86 6 0.0 6 6 87 15 0.0 6 15 88 8 0.0 6 8 89 8 0.0 6 8 90 4 0.0 6 4 91 5 0.0 6 5 92 4 0.0 6 4 93 6 0.0 6 6 94 3 0.0 6 3 95 5 0.0 6 5 96 4 0.0 6 4 97 3 0.0 6 3 98 3 0.0 6 3 99 5 0.0 6 5 100 8 0.0 6 8 101 6 0.0 6 6 102 5 0.0 6 5 103 7 0.0 6 7 104 8 0.0 6 8 105 10 0.0 6 10 106 15 0.0 6 15 107 26 0.0 6 26 108 7 0.0 6 7 109 3 0.0 6 3 110 6 0.0 6 6 111 3 0.0 6 2 1 112 6 0.0 6 6 113 10 0.0 6 10 114 7 0.0 6 7 115 7 0.0 6 7 116 2 0.0 6 2 117 9 0.0 6 9 118 11 0.0 6 11 119 2 0.0 6 2 120 2 0.0 6 2 121 7 0.0 6 7 122 4 0.0 6 4 123 5 0.0 6 5 124 12 0.0 6 12 125 6 0.0 6 6 126 2 0.0 6 2 128 3 0.0 6 3 129 10 0.0 6 10 130 9 0.0 6 9 131 17 0.0 6 17 132 3 0.0 6 3 133 7 0.0 6 7 134 2 0.0 6 2 135 4 0.0 6 4 136 5 0.0 6 5 137 3 0.0 6 3 138 8 0.0 6 8 139 6 0.0 6 6 140 11 0.0 6 11 141 4 0.0 6 4 142 9 0.0 6 8 1 143 7 0.0 6 7 144 8 0.0 6 8 145 10 0.0 6 10 146 25 0.0 6 25 147 11 0.0 6 11 148 9 0.0 6 9 149 9 0.0 6 9 150 2 0.0 6 2 151 10 0.0 6 9 1 152 8 0.0 6 8 153 5 0.0 6 5 154 12 0.0 6 12 155 13 0.0 6 13 156 1 0.0 6 1 157 10 0.0 6 10 158 6 0.0 6 6 159 11 0.0 6 11 160 8 0.0 6 8 161 9 0.0 6 9 162 6 0.0 6 5 1 163 4 0.0 6 4 164 16 0.0 6 16 165 3 0.0 6 3 166 5 0.0 6 5 167 12 0.0 6 12 168 6 0.0 6 6 169 5 0.0 6 5 170 7 0.0 6 7 171 11 0.0 6 11 172 8 0.0 6 8 173 15 0.0 6 15 174 4 0.0 6 4 175 5 0.0 6 5 176 11 0.0 6 11 177 2 0.0 6 2 178 7 0.0 6 7 179 8 0.0 6 8 180 9 0.0 6 9 181 3 0.0 6 3 182 8 0.0 6 8 183 6 0.0 6 6 184 2 0.0 6 2 185 5 0.0 6 5 186 6 0.0 6 6 187 3 0.0 6 3 188 7 0.0 6 7 189 4 0.0 6 4 190 4 0.0 6 4 191 5 0.0 6 5 192 1 0.0 6 1 193 1 0.0 6 1 194 2 0.0 6 2 195 3 0.0 6 3 196 4 0.0 6 4 197 7 0.0 6 7 198 2 0.0 6 2 199 4 0.0 6 4 200 2 0.0 6 2 201 2 0.0 6 2 203 1 0.0 6 1 204 1 0.0 6 1 205 1 0.0 6 1 206 1 0.0 6 1 207 2 0.0 6 2 209 3 0.0 6 3 212 2 0.0 6 2 213 1 0.0 6 1 216 2 0.0 6 2 220 2 0.0 6 2 223 1 0.0 6 1 224 1 0.0 6 1 227 2 0.0 6 2 229 2 0.0 6 2 234 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_10_174_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_10_401_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_10_174_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_10_401_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 6.51 s (109 µs/read; 0.55 M reads/minute). === Summary === Total read pairs processed: 59,685 Read 1 with adapter: 59,683 (100.0%) Read 2 with adapter: 59,659 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 28 (0.0%) Pairs written (passing filters): 59,657 (100.0%) Total basepairs processed: 29,733,400 bp Read 1: 17,050,707 bp Read 2: 12,682,693 bp Total written (filtered): 3,761,912 bp (12.7%) Read 1: 1,851,539 bp Read 2: 1,910,373 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 59642 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 932.6 0 0 2 4 1 233.1 1 1 5 2 58.3 1 2 6 2 14.6 1 0 0 2 7 2 3.6 2 1 0 1 15 1 0.0 4 1 16 3 0.0 4 3 18 1 0.0 5 1 20 5 0.0 6 5 21 3 0.0 6 3 22 5 0.0 6 5 23 4 0.0 6 4 24 3 0.0 6 2 1 25 5 0.0 6 5 26 2 0.0 6 2 27 2 0.0 6 2 28 5 0.0 6 4 1 29 1 0.0 6 1 30 1 0.0 6 1 33 1 0.0 6 1 34 3 0.0 6 3 35 1 0.0 6 1 37 1 0.0 6 1 38 4 0.0 6 4 40 2 0.0 6 2 41 3 0.0 6 3 43 2 0.0 6 2 44 10 0.0 6 9 1 45 3 0.0 6 3 46 1 0.0 6 1 47 1 0.0 6 1 48 1 0.0 6 0 1 49 1 0.0 6 1 50 4 0.0 6 3 0 0 0 1 51 2 0.0 6 2 52 2 0.0 6 1 1 54 2 0.0 6 2 55 1 0.0 6 1 56 1 0.0 6 1 57 3 0.0 6 2 1 58 2 0.0 6 1 1 59 2 0.0 6 1 1 60 2 0.0 6 2 61 1 0.0 6 1 62 2 0.0 6 2 63 1 0.0 6 1 64 5 0.0 6 3 1 1 65 3 0.0 6 3 66 3 0.0 6 3 67 6 0.0 6 6 68 1 0.0 6 1 69 1 0.0 6 1 73 3 0.0 6 2 1 76 1 0.0 6 1 77 4 0.0 6 4 78 6 0.0 6 6 81 1 0.0 6 1 82 1 0.0 6 1 84 2 0.0 6 2 85 1 0.0 6 0 1 86 10 0.0 6 10 87 4 0.0 6 3 1 88 2 0.0 6 2 89 2 0.0 6 2 90 3 0.0 6 1 1 1 91 1 0.0 6 0 1 92 2 0.0 6 2 93 1 0.0 6 1 96 3 0.0 6 2 0 1 97 3 0.0 6 3 98 6 0.0 6 6 100 2 0.0 6 2 101 9 0.0 6 9 102 5 0.0 6 5 103 6 0.0 6 6 105 2 0.0 6 1 0 1 106 3 0.0 6 2 1 107 6 0.0 6 6 108 6 0.0 6 6 109 3 0.0 6 3 110 2 0.0 6 2 111 6 0.0 6 6 112 5 0.0 6 4 1 113 7 0.0 6 7 114 8 0.0 6 8 115 6 0.0 6 6 116 1 0.0 6 1 117 7 0.0 6 7 118 6 0.0 6 6 119 2 0.0 6 2 120 1 0.0 6 1 121 6 0.0 6 4 0 2 122 12 0.0 6 12 123 15 0.0 6 13 0 2 124 10 0.0 6 8 2 125 10 0.0 6 10 126 5 0.0 6 5 127 8 0.0 6 7 1 128 12 0.0 6 10 2 129 8 0.0 6 8 130 8 0.0 6 7 1 131 1 0.0 6 1 132 2 0.0 6 2 133 12 0.0 6 11 1 134 11 0.0 6 10 1 135 18 0.0 6 18 136 12 0.0 6 11 1 137 16 0.0 6 15 1 138 32 0.0 6 30 2 139 11 0.0 6 11 140 22 0.0 6 21 1 141 17 0.0 6 17 142 23 0.0 6 22 1 143 94 0.0 6 94 144 46 0.0 6 43 3 145 45 0.0 6 44 1 146 46 0.0 6 46 147 61 0.0 6 59 2 148 30 0.0 6 29 0 1 149 43 0.0 6 40 3 150 60 0.0 6 60 151 37 0.0 6 37 152 26 0.0 6 26 153 54 0.0 6 53 1 154 22 0.0 6 22 155 33 0.0 6 32 1 156 54 0.0 6 54 157 70 0.0 6 69 1 158 31 0.0 6 30 1 159 43 0.0 6 43 160 64 0.0 6 64 161 70 0.0 6 69 1 162 25 0.0 6 24 1 163 56 0.0 6 54 2 164 27 0.0 6 27 165 30 0.0 6 30 166 21 0.0 6 21 167 53 0.0 6 53 168 49 0.0 6 48 1 169 34 0.0 6 33 1 170 38 0.0 6 38 171 47 0.0 6 46 1 172 129 0.0 6 127 2 173 59 0.0 6 57 2 174 33 0.0 6 31 1 1 175 82 0.0 6 80 2 176 65 0.0 6 64 1 177 78 0.0 6 77 1 178 73 0.0 6 71 2 179 72 0.0 6 70 2 180 68 0.0 6 67 1 181 57 0.0 6 55 2 182 62 0.0 6 60 2 183 65 0.0 6 65 184 88 0.0 6 86 2 185 136 0.0 6 133 3 186 154 0.0 6 147 7 187 112 0.0 6 106 5 1 188 75 0.0 6 75 189 115 0.0 6 108 6 0 0 0 1 190 134 0.0 6 128 5 1 191 197 0.0 6 194 3 192 152 0.0 6 149 2 0 1 193 151 0.0 6 145 6 194 162 0.0 6 159 3 195 199 0.0 6 195 4 196 201 0.0 6 194 7 197 184 0.0 6 180 4 198 274 0.0 6 267 7 199 236 0.0 6 233 3 200 191 0.0 6 184 7 201 190 0.0 6 184 4 2 202 256 0.0 6 251 5 203 286 0.0 6 280 6 204 241 0.0 6 233 8 205 270 0.0 6 264 6 206 183 0.0 6 176 7 207 406 0.0 6 389 17 208 318 0.0 6 309 8 1 209 350 0.0 6 343 7 210 229 0.0 6 228 1 211 146 0.0 6 143 3 212 131 0.0 6 123 5 2 0 0 1 213 240 0.0 6 235 4 0 0 0 0 1 214 191 0.0 6 187 4 215 167 0.0 6 166 1 216 243 0.0 6 241 2 217 379 0.0 6 373 6 218 232 0.0 6 222 10 219 203 0.0 6 199 3 1 220 160 0.0 6 158 2 221 176 0.0 6 172 4 222 218 0.0 6 216 2 223 137 0.0 6 132 4 0 1 224 109 0.0 6 107 2 225 222 0.0 6 214 8 226 595 0.0 6 586 9 227 458 0.0 6 446 11 0 1 228 426 0.0 6 418 8 229 287 0.0 6 277 10 230 321 0.0 6 313 8 231 245 0.0 6 242 3 232 337 0.0 6 325 12 233 352 0.0 6 348 4 234 354 0.0 6 345 9 235 277 0.0 6 272 5 236 140 0.0 6 137 3 237 180 0.0 6 179 1 238 226 0.0 6 217 9 239 268 0.0 6 264 4 240 331 0.0 6 326 4 1 241 579 0.0 6 566 13 242 409 0.0 6 397 12 243 443 0.0 6 433 10 244 336 0.0 6 329 7 245 573 0.0 6 568 5 246 138 0.0 6 137 1 247 211 0.0 6 207 3 1 248 139 0.0 6 136 3 249 465 0.0 6 462 3 250 252 0.0 6 248 4 251 348 0.0 6 340 8 252 310 0.0 6 301 8 0 1 253 222 0.0 6 215 7 254 591 0.0 6 579 12 255 274 0.0 6 269 4 0 0 1 256 238 0.0 6 233 5 257 254 0.0 6 244 9 1 258 365 0.0 6 358 7 259 496 0.0 6 473 23 260 635 0.0 6 624 11 261 181 0.0 6 179 2 262 364 0.0 6 351 13 263 997 0.0 6 972 25 264 355 0.0 6 346 8 0 0 0 0 1 265 395 0.0 6 392 3 266 213 0.0 6 206 7 267 338 0.0 6 332 6 268 293 0.0 6 286 7 269 509 0.0 6 498 11 270 204 0.0 6 196 7 1 271 455 0.0 6 444 11 272 984 0.0 6 965 19 273 398 0.0 6 387 9 2 274 658 0.0 6 642 13 3 275 727 0.0 6 709 17 1 276 1102 0.0 6 1070 30 2 277 1542 0.0 6 1514 27 0 1 278 697 0.0 6 676 19 2 279 395 0.0 6 378 13 4 280 25464 0.0 6 24843 610 11 281 145 0.0 6 124 18 1 2 282 4 0.0 6 4 283 2 0.0 6 1 1 284 1 0.0 6 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 41 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 3 0.0 6 3 73 2 0.0 6 2 74 2 0.0 6 2 77 2 0.0 6 2 83 1 0.0 6 1 85 2 0.0 6 2 88 1 0.0 6 1 95 1 0.0 6 1 103 1 0.0 6 1 104 1 0.0 6 1 105 2 0.0 6 2 106 1 0.0 6 1 108 2 0.0 6 2 111 1 0.0 6 1 118 1 0.0 6 1 119 1 0.0 6 1 121 1 0.0 6 1 122 1 0.0 6 1 123 1 0.0 6 1 138 3 0.0 6 3 147 1 0.0 6 1 152 1 0.0 6 1 156 1 0.0 6 1 163 2 0.0 6 2 164 2 0.0 6 2 186 3 0.0 6 3 222 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 59605 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 932.6 0 0 1 5 1 58.3 1 1 7 2 3.6 2 2 9 2 0.2 2 2 10 4 0.1 3 2 2 11 3 0.0 3 3 12 1 0.0 3 0 1 13 7 0.0 3 6 0 0 1 14 3 0.0 4 2 1 16 8 0.0 4 6 2 17 13 0.0 5 9 4 18 9 0.0 5 8 1 19 9 0.0 5 9 20 39 0.0 6 38 1 21 42 0.0 6 40 2 22 31 0.0 6 28 3 23 14 0.0 6 13 1 24 22 0.0 6 20 2 25 25 0.0 6 22 3 26 12 0.0 6 12 27 16 0.0 6 16 28 9 0.0 6 9 29 16 0.0 6 14 2 30 8 0.0 6 6 2 31 7 0.0 6 7 32 12 0.0 6 10 1 0 0 1 33 8 0.0 6 8 34 9 0.0 6 9 35 4 0.0 6 3 1 36 4 0.0 6 4 37 13 0.0 6 11 1 0 0 0 1 38 9 0.0 6 8 1 39 11 0.0 6 7 4 40 2 0.0 6 0 2 41 10 0.0 6 10 42 5 0.0 6 5 43 12 0.0 6 10 2 44 12 0.0 6 10 1 0 0 1 45 5 0.0 6 4 1 46 28 0.0 6 25 3 47 26 0.0 6 23 3 48 17 0.0 6 15 2 49 16 0.0 6 14 2 50 12 0.0 6 11 1 51 10 0.0 6 9 0 0 0 1 52 26 0.0 6 23 2 1 53 26 0.0 6 24 2 54 19 0.0 6 13 4 1 0 1 55 21 0.0 6 21 56 23 0.0 6 21 2 57 18 0.0 6 17 0 1 58 9 0.0 6 8 1 59 5 0.0 6 3 2 60 12 0.0 6 12 61 9 0.0 6 9 62 13 0.0 6 12 1 63 13 0.0 6 12 1 64 11 0.0 6 9 2 65 10 0.0 6 7 2 1 66 23 0.0 6 20 2 0 0 1 67 96 0.0 6 93 3 68 24 0.0 6 21 2 0 0 1 69 42 0.0 6 41 1 70 20 0.0 6 19 1 71 35 0.0 6 33 2 72 24 0.0 6 21 3 73 12 0.0 6 10 2 74 7 0.0 6 7 75 13 0.0 6 12 1 76 17 0.0 6 16 1 77 19 0.0 6 18 1 78 25 0.0 6 23 1 1 79 19 0.0 6 17 2 80 16 0.0 6 16 81 20 0.0 6 20 82 31 0.0 6 25 6 83 48 0.0 6 46 1 1 84 35 0.0 6 33 2 85 27 0.0 6 26 1 86 16 0.0 6 12 3 1 87 53 0.0 6 47 6 88 24 0.0 6 24 89 29 0.0 6 27 2 90 24 0.0 6 19 4 0 0 1 91 35 0.0 6 30 4 0 0 1 92 18 0.0 6 15 2 1 93 53 0.0 6 47 4 2 94 66 0.0 6 63 2 1 95 30 0.0 6 28 2 96 45 0.0 6 39 4 1 0 1 97 33 0.0 6 31 2 98 39 0.0 6 34 5 99 55 0.0 6 50 1 3 1 100 38 0.0 6 37 1 101 37 0.0 6 36 1 102 71 0.0 6 67 4 103 75 0.0 6 70 5 104 82 0.0 6 76 5 1 105 73 0.0 6 68 5 106 46 0.0 6 44 2 107 34 0.0 6 30 4 108 36 0.0 6 31 4 1 109 51 0.0 6 47 3 1 110 53 0.0 6 48 5 111 56 0.0 6 54 2 112 45 0.0 6 41 3 1 113 42 0.0 6 41 1 114 59 0.0 6 56 2 0 0 1 115 49 0.0 6 46 3 116 61 0.0 6 57 4 117 50 0.0 6 48 2 118 66 0.0 6 61 5 119 76 0.0 6 72 4 120 107 0.0 6 100 5 2 121 79 0.0 6 74 5 122 100 0.0 6 93 6 1 123 70 0.0 6 68 2 124 102 0.0 6 98 3 1 125 123 0.0 6 115 7 0 0 1 126 87 0.0 6 83 2 1 1 127 106 0.0 6 105 1 128 90 0.0 6 89 1 129 48 0.0 6 46 2 130 71 0.0 6 68 3 131 147 0.0 6 138 7 1 1 132 236 0.0 6 227 7 1 0 1 133 158 0.0 6 152 6 134 257 0.0 6 248 9 135 317 0.0 6 308 9 136 134 0.0 6 129 5 137 214 0.0 6 208 6 138 123 0.0 6 114 9 139 239 0.0 6 228 11 140 251 0.0 6 246 5 141 281 0.0 6 275 6 142 328 0.0 6 318 8 1 0 1 143 287 0.0 6 275 12 144 441 0.0 6 430 10 0 0 1 145 223 0.0 6 214 8 0 1 146 206 0.0 6 198 6 1 0 1 147 185 0.0 6 179 5 1 148 246 0.0 6 241 4 1 149 465 0.0 6 451 10 1 0 3 150 708 0.0 6 685 23 151 286 0.0 6 275 9 0 0 2 152 744 0.0 6 730 12 0 1 1 153 431 0.0 6 404 23 2 1 1 154 455 0.0 6 443 10 1 1 155 374 0.0 6 364 9 0 0 1 156 333 0.0 6 315 16 1 1 157 482 0.0 6 459 23 158 487 0.0 6 470 15 0 0 1 0 1 159 199 0.0 6 191 7 0 1 160 420 0.0 6 407 13 161 238 0.0 6 231 7 162 200 0.0 6 191 9 163 386 0.0 6 371 13 1 0 1 164 381 0.0 6 362 16 2 0 0 1 165 398 0.0 6 383 14 1 166 505 0.0 6 486 16 2 0 1 167 238 0.0 6 224 14 168 791 0.0 6 773 16 0 0 0 2 169 203 0.0 6 193 9 1 170 296 0.0 6 284 10 0 0 2 171 676 0.0 6 653 23 172 298 0.0 6 289 9 173 405 0.0 6 394 10 1 174 637 0.0 6 614 18 2 1 1 1 175 947 0.0 6 926 19 0 0 2 176 560 0.0 6 536 22 2 177 612 0.0 6 596 14 0 0 1 0 1 178 515 0.0 6 498 15 1 0 0 1 179 609 0.0 6 588 20 0 1 180 552 0.0 6 530 21 0 0 1 181 638 0.0 6 619 18 1 182 187 0.0 6 178 8 1 183 693 0.0 6 681 12 184 559 0.0 6 537 21 1 185 775 0.0 6 747 22 3 2 0 0 1 186 1428 0.0 6 1385 39 4 187 1242 0.0 6 1213 28 1 188 1218 0.0 6 1186 30 0 0 2 189 1085 0.0 6 1054 30 1 190 1700 0.0 6 1654 43 1 0 2 191 1699 0.0 6 1644 54 1 192 781 0.0 6 755 25 1 193 502 0.0 6 479 21 2 194 1048 0.0 6 1012 33 1 0 2 195 1479 0.0 6 1426 51 2 196 2573 0.0 6 2498 71 3 0 1 197 816 0.0 6 778 34 3 1 198 1597 0.0 6 1539 54 2 1 1 199 2480 0.0 6 2415 60 2 0 3 200 796 0.0 6 765 31 201 1251 0.0 6 1213 38 202 645 0.0 6 620 24 1 203 487 0.0 6 473 13 0 0 1 204 887 0.0 6 858 27 0 0 1 0 1 205 248 0.0 6 237 11 206 283 0.0 6 280 3 207 123 0.0 6 121 2 208 295 0.0 6 288 7 209 322 0.0 6 317 5 210 296 0.0 6 289 6 0 0 1 211 258 0.0 6 248 10 212 494 0.0 6 484 9 0 1 213 229 0.0 6 220 8 1 214 936 0.0 6 913 21 2 215 488 0.0 6 477 11 216 630 0.0 6 613 16 0 1 217 307 0.0 6 299 8 218 240 0.0 6 232 7 0 0 1 219 291 0.0 6 287 4 220 61 0.0 6 55 5 0 1 221 277 0.0 6 266 8 2 0 1 222 442 0.0 6 429 12 1 223 628 0.0 6 612 16 224 182 0.0 6 175 7 225 293 0.0 6 283 10 226 170 0.0 6 164 6 227 216 0.0 6 212 4 228 93 0.0 6 88 4 1 229 275 0.0 6 264 10 0 0 1 230 141 0.0 6 138 2 0 0 1 231 209 0.0 6 203 6 232 277 0.0 6 269 8 233 185 0.0 6 179 6 234 112 0.0 6 110 2 235 61 0.0 6 61 236 9 0.0 6 9 237 19 0.0 6 18 1 238 16 0.0 6 16 239 28 0.0 6 28 240 88 0.0 6 87 1 241 116 0.0 6 112 4 242 129 0.0 6 125 4 243 155 0.0 6 151 4 244 92 0.0 6 89 3 245 37 0.0 6 37 246 64 0.0 6 63 1 247 27 0.0 6 26 1 248 46 0.0 6 45 1 249 4 0.0 6 3 1 250 9 0.0 6 8 1 251 34 0.0 6 31 3 252 9 0.0 6 9 253 1 0.0 6 1 254 3 0.0 6 3 258 1 0.0 6 1 273 1 0.0 6 1 275 1 0.0 6 0 0 0 1 277 4 0.0 6 4 278 34 0.0 6 34 279 45 0.0 6 42 3 281 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 54 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 2 0.0 6 2 73 1 0.0 6 1 77 1 0.0 6 1 78 2 0.0 6 2 79 1 0.0 6 1 82 1 0.0 6 1 83 1 0.0 6 1 84 1 0.0 6 1 85 2 0.0 6 2 86 1 0.0 6 1 88 2 0.0 6 2 89 3 0.0 6 3 90 1 0.0 6 1 92 2 0.0 6 2 96 1 0.0 6 1 100 1 0.0 6 1 101 2 0.0 6 2 104 1 0.0 6 1 108 1 0.0 6 1 111 2 0.0 6 2 112 1 0.0 6 1 119 3 0.0 6 3 120 1 0.0 6 1 124 2 0.0 6 2 138 1 0.0 6 1 139 7 0.0 6 7 140 1 0.0 6 1 147 1 0.0 6 1 153 1 0.0 6 1 155 1 0.0 6 1 161 1 0.0 6 1 163 2 0.0 6 2 164 1 0.0 6 1 178 1 0.0 6 1 198 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_11_175_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_11_402_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_11_175_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_11_402_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 6.04 s (121 µs/read; 0.50 M reads/minute). === Summary === Total read pairs processed: 50,050 Read 1 with adapter: 50,048 (100.0%) Read 2 with adapter: 50,035 (100.0%) == Read fate breakdown == Pairs that were too short: 18 (0.0%) Pairs discarded as untrimmed: 16 (0.0%) Pairs written (passing filters): 50,016 (99.9%) Total basepairs processed: 22,451,468 bp Read 1: 12,358,671 bp Read 2: 10,092,797 bp Total written (filtered): 3,091,869 bp (13.8%) Read 1: 1,606,024 bp Read 2: 1,485,845 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 49005 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 2 195.5 1 0 2 7 2 3.1 2 2 9 2 0.2 2 1 1 15 2 0.0 4 2 16 3 0.0 4 2 1 19 3 0.0 5 1 1 0 0 1 20 42 0.0 6 39 3 21 24 0.0 6 23 1 22 19 0.0 6 19 23 11 0.0 6 10 1 24 11 0.0 6 11 25 9 0.0 6 9 26 10 0.0 6 9 1 27 4 0.0 6 4 28 6 0.0 6 4 1 1 29 3 0.0 6 3 30 1 0.0 6 1 31 4 0.0 6 3 1 32 3 0.0 6 3 33 2 0.0 6 1 1 34 2 0.0 6 2 35 3 0.0 6 3 36 5 0.0 6 5 37 2 0.0 6 2 38 3 0.0 6 3 39 4 0.0 6 4 40 3 0.0 6 3 41 2 0.0 6 2 42 3 0.0 6 3 43 3 0.0 6 2 1 44 2 0.0 6 2 45 3 0.0 6 3 46 1 0.0 6 1 47 3 0.0 6 3 48 2 0.0 6 2 49 3 0.0 6 3 50 3 0.0 6 3 51 3 0.0 6 2 1 52 4 0.0 6 3 0 0 1 53 2 0.0 6 2 54 2 0.0 6 2 55 4 0.0 6 4 56 2 0.0 6 1 1 57 6 0.0 6 6 58 5 0.0 6 5 59 4 0.0 6 4 60 6 0.0 6 6 61 2 0.0 6 1 1 62 3 0.0 6 3 63 4 0.0 6 4 64 1 0.0 6 0 1 65 3 0.0 6 3 66 4 0.0 6 4 67 3 0.0 6 3 68 6 0.0 6 6 70 5 0.0 6 5 71 5 0.0 6 5 72 6 0.0 6 6 73 4 0.0 6 4 74 5 0.0 6 5 75 2 0.0 6 2 76 3 0.0 6 3 77 3 0.0 6 3 78 6 0.0 6 6 79 5 0.0 6 5 80 5 0.0 6 5 81 4 0.0 6 4 82 3 0.0 6 2 1 83 9 0.0 6 9 84 6 0.0 6 6 85 10 0.0 6 10 86 7 0.0 6 7 87 5 0.0 6 5 88 4 0.0 6 4 89 2 0.0 6 2 90 8 0.0 6 7 1 91 2 0.0 6 2 92 4 0.0 6 4 93 6 0.0 6 6 94 2 0.0 6 2 95 4 0.0 6 4 96 4 0.0 6 4 97 12 0.0 6 11 1 98 5 0.0 6 5 99 4 0.0 6 4 100 11 0.0 6 11 101 5 0.0 6 5 102 7 0.0 6 6 1 103 6 0.0 6 6 104 6 0.0 6 6 105 16 0.0 6 16 106 11 0.0 6 10 1 107 10 0.0 6 9 1 108 13 0.0 6 12 0 1 109 12 0.0 6 12 110 11 0.0 6 11 111 16 0.0 6 16 112 11 0.0 6 10 1 113 18 0.0 6 18 114 10 0.0 6 10 115 7 0.0 6 7 116 15 0.0 6 15 117 17 0.0 6 17 118 16 0.0 6 16 119 13 0.0 6 13 120 12 0.0 6 12 121 15 0.0 6 15 122 18 0.0 6 17 1 123 22 0.0 6 21 1 124 11 0.0 6 11 125 18 0.0 6 18 126 18 0.0 6 18 127 19 0.0 6 19 128 19 0.0 6 19 129 21 0.0 6 20 1 130 24 0.0 6 23 1 131 26 0.0 6 26 132 21 0.0 6 21 133 14 0.0 6 13 1 134 27 0.0 6 27 135 24 0.0 6 24 136 32 0.0 6 32 137 25 0.0 6 25 138 26 0.0 6 26 139 48 0.0 6 48 140 160 0.0 6 160 141 198 0.0 6 197 1 142 193 0.0 6 191 2 143 146 0.0 6 145 1 144 462 0.0 6 462 145 254 0.0 6 251 3 146 701 0.0 6 698 3 147 203 0.0 6 202 1 148 64 0.0 6 64 149 69 0.0 6 68 0 0 1 150 239 0.0 6 238 1 151 262 0.0 6 262 152 271 0.0 6 271 153 94 0.0 6 92 1 1 154 267 0.0 6 267 155 368 0.0 6 366 2 156 417 0.0 6 416 1 157 169 0.0 6 168 1 158 189 0.0 6 188 0 0 1 159 502 0.0 6 499 3 160 174 0.0 6 174 161 147 0.0 6 146 1 162 363 0.0 6 362 1 163 226 0.0 6 224 2 164 110 0.0 6 109 1 165 130 0.0 6 130 166 118 0.0 6 117 1 167 121 0.0 6 120 1 168 161 0.0 6 160 0 0 1 169 437 0.0 6 435 0 2 170 203 0.0 6 202 1 171 121 0.0 6 121 172 289 0.0 6 287 2 173 886 0.0 6 884 2 174 340 0.0 6 338 2 175 213 0.0 6 211 2 176 392 0.0 6 390 2 177 268 0.0 6 267 1 178 332 0.0 6 330 2 179 276 0.0 6 274 1 1 180 408 0.0 6 408 181 286 0.0 6 286 182 503 0.0 6 501 2 183 188 0.0 6 187 1 184 181 0.0 6 181 185 680 0.0 6 679 0 0 0 1 186 227 0.0 6 225 2 187 501 0.0 6 501 188 135 0.0 6 135 189 97 0.0 6 96 1 190 141 0.0 6 139 2 191 262 0.0 6 262 192 392 0.0 6 388 2 1 0 1 193 241 0.0 6 235 5 0 1 194 170 0.0 6 166 4 195 316 0.0 6 316 196 215 0.0 6 213 2 197 243 0.0 6 241 2 198 545 0.0 6 542 3 199 413 0.0 6 407 5 0 0 1 200 565 0.0 6 564 1 201 234 0.0 6 232 2 202 236 0.0 6 235 1 203 189 0.0 6 185 4 204 333 0.0 6 327 6 205 192 0.0 6 189 2 0 0 0 1 206 249 0.0 6 246 2 0 1 207 338 0.0 6 335 3 208 269 0.0 6 266 3 209 447 0.0 6 441 6 210 309 0.0 6 305 4 211 354 0.0 6 347 5 0 0 0 1 1 212 346 0.0 6 339 7 213 392 0.0 6 382 9 0 1 214 446 0.0 6 438 7 0 0 1 215 469 0.0 6 465 4 216 820 0.0 6 812 6 1 0 0 0 1 217 455 0.0 6 451 4 218 471 0.0 6 468 3 219 822 0.0 6 821 1 220 376 0.0 6 375 1 221 382 0.0 6 381 1 222 268 0.0 6 264 4 223 374 0.0 6 371 2 0 1 224 329 0.0 6 319 9 0 0 0 0 1 225 274 0.0 6 269 5 226 452 0.0 6 445 6 0 0 0 1 227 400 0.0 6 393 7 228 290 0.0 6 287 3 229 314 0.0 6 310 4 230 298 0.0 6 296 1 0 1 231 284 0.0 6 279 5 232 314 0.0 6 313 1 233 317 0.0 6 315 2 234 276 0.0 6 275 1 235 331 0.0 6 327 3 0 0 0 0 1 236 274 0.0 6 271 3 237 327 0.0 6 326 1 238 190 0.0 6 190 239 295 0.0 6 293 2 240 358 0.0 6 352 6 241 215 0.0 6 212 3 242 251 0.0 6 249 2 243 166 0.0 6 164 2 244 238 0.0 6 238 245 148 0.0 6 143 5 246 237 0.0 6 237 247 308 0.0 6 304 4 248 205 0.0 6 203 2 249 276 0.0 6 271 5 250 284 0.0 6 279 5 251 214 0.0 6 205 8 1 252 536 0.0 6 531 5 253 293 0.0 6 290 3 254 228 0.0 6 225 2 0 0 0 0 1 255 211 0.0 6 206 5 256 290 0.0 6 284 6 257 344 0.0 6 338 5 0 1 258 308 0.0 6 297 10 1 259 545 0.0 6 534 11 260 295 0.0 6 283 11 1 261 544 0.0 6 535 9 262 209 0.0 6 202 7 263 424 0.0 6 420 4 264 200 0.0 6 197 2 1 265 151 0.0 6 148 3 266 284 0.0 6 270 13 1 267 298 0.0 6 293 5 268 183 0.0 6 180 3 269 266 0.0 6 262 4 270 258 0.0 6 247 10 1 271 141 0.0 6 135 6 272 129 0.0 6 126 3 273 99 0.0 6 98 1 274 110 0.0 6 109 1 275 215 0.0 6 209 6 276 466 0.0 6 444 21 1 277 355 0.0 6 336 15 4 278 559 0.0 6 536 23 279 4445 0.0 6 4339 103 3 280 1624 0.0 6 1585 37 1 0 0 0 1 281 15 0.0 6 14 1 282 1 0.0 6 1 284 1 0.0 6 1 285 2 0.0 6 2 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1043 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 9 0.0 6 9 63 1 0.0 6 1 70 1 0.0 6 1 71 9 0.0 6 9 72 5 0.0 6 5 73 4 0.0 6 4 74 5 0.0 6 5 75 13 0.0 6 13 76 16 0.0 6 16 77 27 0.0 6 27 78 4 0.0 6 4 79 3 0.0 6 3 80 2 0.0 6 2 81 11 0.0 6 11 82 8 0.0 6 8 83 9 0.0 6 9 84 4 0.0 6 4 85 12 0.0 6 12 86 21 0.0 6 21 87 11 0.0 6 11 88 7 0.0 6 7 89 5 0.0 6 5 90 17 0.0 6 17 91 2 0.0 6 2 92 4 0.0 6 4 93 13 0.0 6 13 94 2 0.0 6 2 95 2 0.0 6 2 97 2 0.0 6 2 98 2 0.0 6 2 99 3 0.0 6 3 100 13 0.0 6 13 101 6 0.0 6 6 102 5 0.0 6 5 103 18 0.0 6 18 104 33 0.0 6 33 105 6 0.0 6 6 106 10 0.0 6 10 107 9 0.0 6 9 108 11 0.0 6 11 109 8 0.0 6 8 110 13 0.0 6 13 111 12 0.0 6 12 112 13 0.0 6 13 113 15 0.0 6 15 114 4 0.0 6 4 115 4 0.0 6 4 116 24 0.0 6 24 117 3 0.0 6 3 118 12 0.0 6 12 119 4 0.0 6 3 0 0 0 0 0 1 121 3 0.0 6 3 122 9 0.0 6 9 123 14 0.0 6 13 1 124 2 0.0 6 2 125 4 0.0 6 4 126 12 0.0 6 12 127 5 0.0 6 5 128 5 0.0 6 5 129 10 0.0 6 10 130 5 0.0 6 5 131 8 0.0 6 8 132 1 0.0 6 1 133 1 0.0 6 1 134 1 0.0 6 1 135 3 0.0 6 3 137 2 0.0 6 2 138 2 0.0 6 2 139 6 0.0 6 6 140 3 0.0 6 3 141 4 0.0 6 4 143 1 0.0 6 1 145 1 0.0 6 1 146 7 0.0 6 7 147 10 0.0 6 10 148 5 0.0 6 5 149 15 0.0 6 15 150 42 0.0 6 42 151 2 0.0 6 2 152 3 0.0 6 3 153 5 0.0 6 5 154 5 0.0 6 5 155 2 0.0 6 2 156 1 0.0 6 1 157 5 0.0 6 5 158 3 0.0 6 3 160 2 0.0 6 2 161 1 0.0 6 1 162 4 0.0 6 4 163 13 0.0 6 13 164 5 0.0 6 5 165 2 0.0 6 2 166 8 0.0 6 8 167 8 0.0 6 8 168 13 0.0 6 13 169 5 0.0 6 5 170 7 0.0 6 7 172 3 0.0 6 3 174 5 0.0 6 5 175 8 0.0 6 8 177 1 0.0 6 1 178 1 0.0 6 1 179 10 0.0 6 10 180 25 0.0 6 25 181 1 0.0 6 1 182 1 0.0 6 1 184 21 0.0 6 21 185 24 0.0 6 24 186 9 0.0 6 9 187 10 0.0 6 10 188 2 0.0 6 2 189 5 0.0 6 5 190 4 0.0 6 4 191 6 0.0 6 6 194 1 0.0 6 1 196 1 0.0 6 1 197 3 0.0 6 3 199 14 0.0 6 14 200 5 0.0 6 5 202 2 0.0 6 2 203 7 0.0 6 7 204 2 0.0 6 2 205 4 0.0 6 4 206 11 0.0 6 11 209 1 0.0 6 1 210 4 0.0 6 4 211 9 0.0 6 9 213 2 0.0 6 2 214 3 0.0 6 3 216 2 0.0 6 2 217 19 0.0 6 19 218 3 0.0 6 3 219 3 0.0 6 3 220 2 0.0 6 2 221 6 0.0 6 6 222 5 0.0 6 5 223 1 0.0 6 1 224 1 0.0 6 1 225 1 0.0 6 1 226 1 0.0 6 1 227 5 0.0 6 5 228 4 0.0 6 4 229 1 0.0 6 1 230 2 0.0 6 2 231 5 0.0 6 5 232 4 0.0 6 4 234 1 0.0 6 1 235 3 0.0 6 3 236 1 0.0 6 1 244 2 0.0 6 2 247 2 0.0 6 2 248 6 0.0 6 6 251 2 0.0 6 2 252 2 0.0 6 2 253 1 0.0 6 1 254 1 0.0 6 1 262 2 0.0 6 2 264 1 0.0 6 1 265 2 0.0 6 2 266 2 0.0 6 2 267 4 0.0 6 3 1 268 3 0.0 6 3 269 1 0.0 6 1 270 1 0.0 6 1 292 1 0.0 6 1 293 3 0.0 6 3 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 49103 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 12.2 1 1 11 3 0.0 3 3 12 1 0.0 3 1 13 6 0.0 3 2 3 0 1 14 4 0.0 4 4 15 2 0.0 4 2 16 3 0.0 4 3 17 7 0.0 5 7 19 18 0.0 5 17 1 20 39 0.0 6 38 1 21 21 0.0 6 19 2 22 21 0.0 6 21 23 2 0.0 6 2 24 5 0.0 6 5 25 7 0.0 6 7 26 10 0.0 6 9 1 27 9 0.0 6 9 28 7 0.0 6 7 29 2 0.0 6 2 30 5 0.0 6 4 0 0 0 0 1 31 5 0.0 6 5 32 4 0.0 6 2 2 33 6 0.0 6 6 34 9 0.0 6 8 1 35 6 0.0 6 5 1 36 5 0.0 6 4 0 0 0 1 37 5 0.0 6 5 38 9 0.0 6 9 39 4 0.0 6 4 40 4 0.0 6 3 1 41 4 0.0 6 4 42 6 0.0 6 6 43 6 0.0 6 6 44 6 0.0 6 6 45 11 0.0 6 10 1 46 5 0.0 6 4 1 47 7 0.0 6 7 48 9 0.0 6 7 2 49 6 0.0 6 5 1 50 10 0.0 6 10 51 10 0.0 6 8 1 0 0 1 52 6 0.0 6 6 53 6 0.0 6 6 54 7 0.0 6 7 55 8 0.0 6 7 1 56 11 0.0 6 10 1 57 12 0.0 6 11 1 58 9 0.0 6 6 3 59 5 0.0 6 4 0 1 60 11 0.0 6 10 0 1 61 6 0.0 6 6 62 12 0.0 6 9 0 0 2 1 63 10 0.0 6 9 1 64 7 0.0 6 7 65 9 0.0 6 8 1 66 12 0.0 6 12 67 12 0.0 6 11 0 0 1 68 24 0.0 6 22 1 0 1 69 25 0.0 6 25 70 103 0.0 6 100 3 71 21 0.0 6 18 2 1 72 40 0.0 6 39 1 73 23 0.0 6 20 2 0 0 1 74 26 0.0 6 26 75 14 0.0 6 13 1 76 17 0.0 6 17 77 22 0.0 6 20 1 1 78 16 0.0 6 14 1 1 79 17 0.0 6 17 80 24 0.0 6 23 1 81 27 0.0 6 25 1 0 1 82 18 0.0 6 16 2 83 24 0.0 6 22 2 84 21 0.0 6 18 2 1 85 25 0.0 6 22 1 1 1 86 56 0.0 6 55 1 87 28 0.0 6 28 88 19 0.0 6 16 2 0 0 1 89 18 0.0 6 16 1 0 0 0 0 1 90 26 0.0 6 24 1 0 1 91 12 0.0 6 10 1 1 92 27 0.0 6 26 1 93 23 0.0 6 22 1 94 35 0.0 6 34 1 95 37 0.0 6 35 1 1 96 34 0.0 6 34 97 33 0.0 6 31 1 0 0 1 98 33 0.0 6 33 99 43 0.0 6 37 5 0 0 1 100 36 0.0 6 35 1 101 39 0.0 6 36 3 102 44 0.0 6 42 2 103 34 0.0 6 32 1 0 0 1 104 39 0.0 6 39 105 40 0.0 6 38 1 1 106 56 0.0 6 52 4 107 76 0.0 6 75 1 108 49 0.0 6 48 1 109 42 0.0 6 42 110 50 0.0 6 49 1 111 56 0.0 6 52 3 0 0 0 1 112 42 0.0 6 38 4 113 65 0.0 6 63 2 114 65 0.0 6 63 2 115 61 0.0 6 59 2 116 60 0.0 6 58 1 1 117 65 0.0 6 63 2 118 79 0.0 6 78 0 0 0 1 119 71 0.0 6 68 3 120 92 0.0 6 89 2 1 121 88 0.0 6 86 2 122 87 0.0 6 83 3 0 0 1 123 84 0.0 6 82 2 124 100 0.0 6 97 2 0 0 0 1 125 70 0.0 6 68 1 0 0 1 126 78 0.0 6 75 2 0 1 127 90 0.0 6 89 1 128 133 0.0 6 127 4 0 1 1 129 111 0.0 6 108 3 130 125 0.0 6 123 1 1 131 123 0.0 6 121 1 1 132 162 0.0 6 161 0 0 0 0 1 133 162 0.0 6 160 0 1 0 0 0 1 134 176 0.0 6 173 3 135 227 0.0 6 219 7 1 136 195 0.0 6 188 6 1 137 217 0.0 6 213 4 138 264 0.0 6 261 3 139 300 0.0 6 295 3 1 0 1 140 370 0.0 6 362 6 0 0 2 141 377 0.0 6 371 5 0 0 1 142 341 0.0 6 333 6 0 2 143 577 0.0 6 568 8 1 144 528 0.0 6 524 4 145 824 0.0 6 815 8 0 0 1 146 413 0.0 6 410 3 147 347 0.0 6 341 6 148 382 0.0 6 372 8 1 0 1 149 547 0.0 6 540 6 1 150 544 0.0 6 534 9 0 0 1 151 661 0.0 6 652 6 2 1 152 636 0.0 6 620 15 0 0 1 153 900 0.0 6 885 14 0 0 0 1 154 766 0.0 6 744 18 2 0 1 1 155 900 0.0 6 884 13 0 0 2 0 1 156 728 0.0 6 715 10 1 0 1 0 1 157 620 0.0 6 609 10 1 158 799 0.0 6 795 4 159 524 0.0 6 512 11 1 160 607 0.0 6 597 9 1 161 641 0.0 6 634 7 162 541 0.0 6 530 10 0 0 1 163 559 0.0 6 550 7 2 164 577 0.0 6 571 4 1 0 1 165 473 0.0 6 463 9 1 166 579 0.0 6 570 7 0 1 0 1 167 604 0.0 6 598 5 0 0 0 1 168 623 0.0 6 615 7 0 1 169 616 0.0 6 604 10 0 0 0 2 170 442 0.0 6 437 5 171 753 0.0 6 742 8 0 0 3 172 631 0.0 6 629 1 0 0 1 173 542 0.0 6 533 8 1 174 547 0.0 6 537 9 1 175 671 0.0 6 662 9 176 583 0.0 6 580 2 0 1 177 676 0.0 6 666 9 0 0 1 178 581 0.0 6 571 8 0 0 2 179 642 0.0 6 633 9 180 549 0.0 6 543 5 0 1 181 628 0.0 6 618 10 182 424 0.0 6 419 4 0 0 1 183 453 0.0 6 449 4 184 486 0.0 6 483 3 185 323 0.0 6 311 11 0 0 1 186 677 0.0 6 668 7 1 1 187 600 0.0 6 585 14 0 0 1 188 688 0.0 6 669 19 189 548 0.0 6 538 10 190 612 0.0 6 599 13 191 605 0.0 6 589 15 0 0 1 192 456 0.0 6 449 6 0 0 0 1 193 498 0.0 6 485 13 194 571 0.0 6 557 11 2 1 195 409 0.0 6 399 9 0 0 1 196 631 0.0 6 618 13 197 283 0.0 6 276 7 198 520 0.0 6 507 13 199 394 0.0 6 390 4 200 474 0.0 6 459 15 201 435 0.0 6 426 8 0 1 202 346 0.0 6 339 5 0 0 2 203 313 0.0 6 300 12 0 1 204 307 0.0 6 304 2 0 0 1 205 311 0.0 6 301 9 1 206 244 0.0 6 240 4 207 275 0.0 6 267 8 208 444 0.0 6 433 10 1 209 134 0.0 6 128 6 210 221 0.0 6 216 5 211 306 0.0 6 293 12 0 0 1 212 263 0.0 6 254 7 1 0 1 213 107 0.0 6 103 4 214 242 0.0 6 234 8 215 293 0.0 6 287 5 0 1 216 312 0.0 6 302 10 217 209 0.0 6 205 3 1 218 192 0.0 6 185 6 1 219 126 0.0 6 125 1 220 113 0.0 6 109 3 0 0 1 221 107 0.0 6 102 5 222 110 0.0 6 106 4 223 59 0.0 6 57 1 0 1 224 65 0.0 6 63 2 225 113 0.0 6 104 8 1 226 179 0.0 6 169 10 227 317 0.0 6 300 14 1 2 228 202 0.0 6 192 10 229 210 0.0 6 198 12 230 396 0.0 6 382 13 1 231 209 0.0 6 204 5 232 477 0.0 6 468 8 0 0 0 1 233 59 0.0 6 54 5 234 142 0.0 6 138 4 235 112 0.0 6 111 1 236 87 0.0 6 85 1 0 1 237 111 0.0 6 107 3 1 238 45 0.0 6 44 1 239 10 0.0 6 10 240 26 0.0 6 25 1 241 67 0.0 6 66 1 242 43 0.0 6 39 3 0 1 243 24 0.0 6 23 1 244 43 0.0 6 41 2 245 123 0.0 6 121 2 246 70 0.0 6 68 2 247 57 0.0 6 55 2 248 32 0.0 6 30 2 249 69 0.0 6 66 3 250 45 0.0 6 44 1 251 97 0.0 6 96 1 252 68 0.0 6 66 2 253 11 0.0 6 11 254 120 0.0 6 117 3 255 28 0.0 6 27 0 0 1 256 26 0.0 6 26 257 3 0.0 6 3 258 1 0.0 6 1 260 1 0.0 6 1 263 1 0.0 6 1 280 4 0.0 6 4 281 19 0.0 6 19 282 103 0.0 6 102 0 0 0 1 284 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 932 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 64 1 0.0 6 1 68 1 0.0 6 1 69 7 0.0 6 7 70 7 0.0 6 7 71 6 0.0 6 5 1 72 4 0.0 6 4 73 14 0.0 6 14 74 14 0.0 6 14 75 22 0.0 6 21 1 76 3 0.0 6 3 78 3 0.0 6 3 79 3 0.0 6 2 0 0 0 1 80 7 0.0 6 7 81 11 0.0 6 11 82 5 0.0 6 5 83 8 0.0 6 8 84 21 0.0 6 21 85 10 0.0 6 10 86 5 0.0 6 4 0 0 1 87 7 0.0 6 7 88 20 0.0 6 19 0 0 0 1 89 2 0.0 6 2 90 5 0.0 6 5 91 17 0.0 6 17 92 11 0.0 6 10 1 93 2 0.0 6 2 94 3 0.0 6 3 95 4 0.0 6 4 96 1 0.0 6 0 1 97 8 0.0 6 8 98 18 0.0 6 18 99 2 0.0 6 2 100 1 0.0 6 1 101 13 0.0 6 13 102 41 0.0 6 41 103 12 0.0 6 12 104 6 0.0 6 6 105 11 0.0 6 11 106 6 0.0 6 6 107 18 0.0 6 18 108 10 0.0 6 9 1 109 10 0.0 6 10 110 15 0.0 6 15 111 16 0.0 6 16 112 2 0.0 6 2 113 6 0.0 6 6 114 39 0.0 6 39 115 6 0.0 6 6 116 15 0.0 6 15 119 3 0.0 6 3 120 8 0.0 6 8 121 17 0.0 6 17 122 2 0.0 6 2 123 8 0.0 6 8 124 13 0.0 6 13 126 2 0.0 6 2 127 4 0.0 6 4 128 3 0.0 6 2 1 129 20 0.0 6 20 130 8 0.0 6 8 131 1 0.0 6 1 132 2 0.0 6 2 133 4 0.0 6 4 135 1 0.0 6 1 136 5 0.0 6 5 137 5 0.0 6 5 138 10 0.0 6 10 140 1 0.0 6 1 141 1 0.0 6 1 142 1 0.0 6 0 0 0 0 1 143 2 0.0 6 2 144 3 0.0 6 3 145 6 0.0 6 6 146 4 0.0 6 4 147 13 0.0 6 13 148 34 0.0 6 34 149 1 0.0 6 1 150 1 0.0 6 1 151 3 0.0 6 3 152 11 0.0 6 11 153 8 0.0 6 8 154 1 0.0 6 1 155 3 0.0 6 3 156 2 0.0 6 2 160 11 0.0 6 11 161 15 0.0 6 15 162 8 0.0 6 8 164 7 0.0 6 7 165 10 0.0 6 10 166 18 0.0 6 18 167 2 0.0 6 2 168 9 0.0 6 9 169 1 0.0 6 1 170 3 0.0 6 3 171 2 0.0 6 2 172 5 0.0 6 5 173 8 0.0 6 8 175 1 0.0 6 1 177 10 0.0 6 10 178 26 0.0 6 26 179 1 0.0 6 1 180 1 0.0 6 1 182 6 0.0 6 6 183 22 0.0 6 22 184 3 0.0 6 3 185 11 0.0 6 11 186 3 0.0 6 2 1 187 6 0.0 6 6 188 1 0.0 6 1 189 10 0.0 6 10 192 1 0.0 6 1 194 2 0.0 6 2 196 1 0.0 6 1 197 2 0.0 6 2 198 3 0.0 6 3 201 2 0.0 6 2 204 2 0.0 6 2 209 2 0.0 6 2 211 1 0.0 6 1 213 1 0.0 6 1 214 6 0.0 6 6 215 14 0.0 6 14 216 1 0.0 6 1 219 1 0.0 6 1 220 1 0.0 6 1 229 1 0.0 6 1 230 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_12_176_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_12_403_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_12_176_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_12_403_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.83 s (168 µs/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 34,612 Read 1 with adapter: 34,612 (100.0%) Read 2 with adapter: 34,587 (99.9%) == Read fate breakdown == Pairs that were too short: 9 (0.0%) Pairs discarded as untrimmed: 25 (0.1%) Pairs written (passing filters): 34,578 (99.9%) Total basepairs processed: 15,141,439 bp Read 1: 8,465,816 bp Read 2: 6,675,623 bp Total written (filtered): 2,195,173 bp (14.5%) Read 1: 1,103,905 bp Read 2: 1,091,268 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 33952 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 540.8 0 1 1 4 1 135.2 1 0 1 6 1 8.5 1 0 0 1 7 1 2.1 2 0 0 1 10 1 0.0 3 1 16 1 0.0 4 1 19 1 0.0 5 0 1 20 40 0.0 6 39 1 21 12 0.0 6 12 22 18 0.0 6 17 1 23 8 0.0 6 8 24 10 0.0 6 10 25 8 0.0 6 8 26 8 0.0 6 8 27 6 0.0 6 6 28 3 0.0 6 3 29 2 0.0 6 2 30 1 0.0 6 1 31 2 0.0 6 2 32 4 0.0 6 4 33 5 0.0 6 5 35 3 0.0 6 3 36 3 0.0 6 3 37 1 0.0 6 0 1 39 6 0.0 6 6 40 2 0.0 6 2 41 1 0.0 6 1 42 3 0.0 6 3 43 4 0.0 6 4 44 4 0.0 6 4 45 5 0.0 6 4 0 0 0 1 46 1 0.0 6 1 48 4 0.0 6 4 49 2 0.0 6 2 50 2 0.0 6 2 51 2 0.0 6 2 52 2 0.0 6 2 53 3 0.0 6 3 54 3 0.0 6 3 55 1 0.0 6 1 56 2 0.0 6 1 1 57 2 0.0 6 2 58 1 0.0 6 1 60 1 0.0 6 1 61 3 0.0 6 3 62 2 0.0 6 1 0 1 63 2 0.0 6 2 64 3 0.0 6 2 1 66 4 0.0 6 4 68 1 0.0 6 1 69 4 0.0 6 4 70 6 0.0 6 6 71 1 0.0 6 1 72 2 0.0 6 2 73 1 0.0 6 1 74 4 0.0 6 3 1 75 7 0.0 6 7 76 1 0.0 6 1 77 5 0.0 6 5 78 1 0.0 6 1 79 6 0.0 6 6 80 2 0.0 6 2 81 3 0.0 6 3 82 5 0.0 6 5 83 8 0.0 6 8 84 5 0.0 6 5 85 4 0.0 6 4 86 2 0.0 6 2 87 4 0.0 6 4 88 2 0.0 6 2 89 4 0.0 6 3 1 90 2 0.0 6 2 91 4 0.0 6 4 92 4 0.0 6 4 93 3 0.0 6 3 94 3 0.0 6 3 95 4 0.0 6 4 96 4 0.0 6 4 97 3 0.0 6 3 98 2 0.0 6 2 99 2 0.0 6 2 100 3 0.0 6 3 101 8 0.0 6 8 102 4 0.0 6 4 103 3 0.0 6 3 104 4 0.0 6 3 1 105 9 0.0 6 8 0 1 106 9 0.0 6 9 107 10 0.0 6 10 108 9 0.0 6 9 109 6 0.0 6 6 110 12 0.0 6 12 111 9 0.0 6 9 112 11 0.0 6 10 0 1 113 17 0.0 6 17 114 15 0.0 6 14 0 0 0 0 1 115 13 0.0 6 13 116 9 0.0 6 9 117 9 0.0 6 9 118 11 0.0 6 11 119 13 0.0 6 12 1 120 7 0.0 6 7 121 9 0.0 6 8 1 122 10 0.0 6 10 123 16 0.0 6 15 1 124 13 0.0 6 13 125 12 0.0 6 12 126 5 0.0 6 5 127 10 0.0 6 10 128 14 0.0 6 14 129 15 0.0 6 15 130 9 0.0 6 9 131 13 0.0 6 13 132 18 0.0 6 16 2 133 13 0.0 6 13 134 22 0.0 6 22 135 22 0.0 6 21 1 136 24 0.0 6 23 1 137 21 0.0 6 21 138 26 0.0 6 26 139 22 0.0 6 21 1 140 28 0.0 6 28 141 47 0.0 6 46 1 142 120 0.0 6 118 2 143 162 0.0 6 160 2 144 142 0.0 6 141 0 0 1 145 198 0.0 6 198 146 227 0.0 6 227 147 207 0.0 6 206 1 148 197 0.0 6 197 149 244 0.0 6 244 150 286 0.0 6 285 1 151 248 0.0 6 248 152 312 0.0 6 312 153 199 0.0 6 198 1 154 109 0.0 6 109 155 173 0.0 6 173 156 172 0.0 6 171 1 157 161 0.0 6 161 158 166 0.0 6 165 1 159 136 0.0 6 136 160 166 0.0 6 166 161 170 0.0 6 170 162 150 0.0 6 150 163 174 0.0 6 173 1 164 154 0.0 6 151 3 165 116 0.0 6 115 0 0 1 166 149 0.0 6 148 1 167 185 0.0 6 184 1 168 207 0.0 6 206 1 169 156 0.0 6 154 2 170 271 0.0 6 270 1 171 220 0.0 6 219 1 172 124 0.0 6 123 1 173 170 0.0 6 170 174 202 0.0 6 202 175 153 0.0 6 152 1 176 144 0.0 6 142 2 177 204 0.0 6 202 2 178 121 0.0 6 121 179 129 0.0 6 129 180 299 0.0 6 299 181 291 0.0 6 288 2 1 182 185 0.0 6 185 183 173 0.0 6 173 184 220 0.0 6 216 3 0 0 0 0 1 185 200 0.0 6 200 186 155 0.0 6 155 187 160 0.0 6 159 1 188 141 0.0 6 140 1 189 158 0.0 6 158 190 165 0.0 6 164 1 191 148 0.0 6 147 1 192 151 0.0 6 149 2 193 174 0.0 6 173 1 194 252 0.0 6 249 3 195 270 0.0 6 266 4 196 257 0.0 6 255 1 0 0 0 1 197 341 0.0 6 335 6 198 391 0.0 6 391 199 407 0.0 6 407 200 311 0.0 6 306 5 201 299 0.0 6 299 202 401 0.0 6 401 203 323 0.0 6 322 1 204 364 0.0 6 363 1 205 323 0.0 6 319 3 0 0 0 0 1 206 406 0.0 6 404 1 1 207 422 0.0 6 417 5 208 331 0.0 6 330 1 209 347 0.0 6 344 2 0 1 210 262 0.0 6 261 1 211 279 0.0 6 278 1 212 313 0.0 6 311 2 213 389 0.0 6 387 2 214 294 0.0 6 291 1 0 2 215 332 0.0 6 330 0 0 0 2 216 391 0.0 6 388 3 217 345 0.0 6 344 0 0 1 218 405 0.0 6 402 2 1 219 354 0.0 6 353 1 220 292 0.0 6 289 3 221 248 0.0 6 247 1 222 234 0.0 6 233 1 223 253 0.0 6 252 1 224 256 0.0 6 255 1 225 280 0.0 6 280 226 322 0.0 6 320 2 227 333 0.0 6 327 5 0 1 228 339 0.0 6 339 229 237 0.0 6 235 2 230 237 0.0 6 236 1 231 263 0.0 6 260 2 1 232 228 0.0 6 227 1 233 259 0.0 6 256 3 234 307 0.0 6 305 2 235 229 0.0 6 229 236 215 0.0 6 213 2 237 213 0.0 6 213 238 245 0.0 6 242 3 239 244 0.0 6 243 1 240 236 0.0 6 234 2 241 215 0.0 6 213 2 242 200 0.0 6 197 2 0 0 1 243 199 0.0 6 198 1 244 187 0.0 6 185 2 245 179 0.0 6 178 1 246 156 0.0 6 156 247 111 0.0 6 110 0 1 248 104 0.0 6 104 249 141 0.0 6 141 250 107 0.0 6 106 1 251 113 0.0 6 111 2 252 131 0.0 6 131 253 98 0.0 6 98 254 137 0.0 6 137 255 84 0.0 6 83 1 256 87 0.0 6 86 1 257 106 0.0 6 105 1 258 123 0.0 6 121 2 259 113 0.0 6 113 260 104 0.0 6 104 261 73 0.0 6 71 2 262 74 0.0 6 74 263 108 0.0 6 107 1 264 65 0.0 6 64 1 265 69 0.0 6 69 266 62 0.0 6 62 267 57 0.0 6 57 268 64 0.0 6 64 269 80 0.0 6 77 3 270 63 0.0 6 60 2 0 0 0 1 271 59 0.0 6 58 1 272 111 0.0 6 110 1 273 71 0.0 6 68 2 0 1 274 104 0.0 6 103 1 275 63 0.0 6 63 276 123 0.0 6 119 3 0 1 277 280 0.0 6 277 3 278 156 0.0 6 155 1 279 399 0.0 6 394 5 280 4534 0.0 6 4444 90 281 42 0.0 6 40 1 0 1 282 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 660 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 5 0.0 6 4 1 70 2 0.0 6 2 71 3 0.0 6 3 72 10 0.0 6 10 73 6 0.0 6 6 74 5 0.0 6 5 75 4 0.0 6 4 76 4 0.0 6 4 77 7 0.0 6 7 78 8 0.0 6 8 79 8 0.0 6 8 80 6 0.0 6 6 81 12 0.0 6 12 82 2 0.0 6 2 83 4 0.0 6 4 84 5 0.0 6 5 85 4 0.0 6 4 86 3 0.0 6 3 87 2 0.0 6 2 88 2 0.0 6 2 89 6 0.0 6 6 90 1 0.0 6 1 91 2 0.0 6 2 92 2 0.0 6 2 93 2 0.0 6 2 94 1 0.0 6 1 95 6 0.0 6 6 96 2 0.0 6 2 97 4 0.0 6 4 98 2 0.0 6 2 99 13 0.0 6 13 100 4 0.0 6 4 101 2 0.0 6 2 102 3 0.0 6 3 103 4 0.0 6 3 1 104 7 0.0 6 7 106 5 0.0 6 5 107 1 0.0 6 1 108 3 0.0 6 3 109 9 0.0 6 9 110 6 0.0 6 6 111 3 0.0 6 3 112 3 0.0 6 3 113 4 0.0 6 4 114 3 0.0 6 3 115 1 0.0 6 1 116 3 0.0 6 3 117 5 0.0 6 5 120 2 0.0 6 2 121 3 0.0 6 3 122 5 0.0 6 5 123 4 0.0 6 4 124 8 0.0 6 8 125 1 0.0 6 1 126 3 0.0 6 3 127 2 0.0 6 2 128 4 0.0 6 4 129 5 0.0 6 5 130 5 0.0 6 5 131 9 0.0 6 9 132 6 0.0 6 6 133 4 0.0 6 4 134 7 0.0 6 7 135 8 0.0 6 8 136 2 0.0 6 2 137 3 0.0 6 3 138 6 0.0 6 6 139 3 0.0 6 3 140 5 0.0 6 5 141 2 0.0 6 2 142 6 0.0 6 6 143 2 0.0 6 2 144 5 0.0 6 5 145 2 0.0 6 2 146 5 0.0 6 5 147 6 0.0 6 6 148 4 0.0 6 4 149 6 0.0 6 6 150 4 0.0 6 4 151 2 0.0 6 2 152 2 0.0 6 2 153 2 0.0 6 2 154 5 0.0 6 5 155 1 0.0 6 1 156 2 0.0 6 2 157 4 0.0 6 4 158 7 0.0 6 7 159 1 0.0 6 1 162 3 0.0 6 3 163 5 0.0 6 5 164 5 0.0 6 5 165 5 0.0 6 5 166 7 0.0 6 7 167 7 0.0 6 7 168 11 0.0 6 11 169 7 0.0 6 7 170 9 0.0 6 9 171 1 0.0 6 1 172 4 0.0 6 4 173 1 0.0 6 1 174 1 0.0 6 1 175 1 0.0 6 1 176 4 0.0 6 4 177 1 0.0 6 1 178 3 0.0 6 3 179 6 0.0 6 6 180 1 0.0 6 1 181 8 0.0 6 8 182 1 0.0 6 1 183 1 0.0 6 1 184 1 0.0 6 1 185 4 0.0 6 4 186 7 0.0 6 7 187 7 0.0 6 7 188 3 0.0 6 3 189 3 0.0 6 3 190 5 0.0 6 5 191 1 0.0 6 1 192 2 0.0 6 2 193 7 0.0 6 7 194 5 0.0 6 5 195 2 0.0 6 2 196 5 0.0 6 5 197 7 0.0 6 7 198 8 0.0 6 8 199 1 0.0 6 1 200 2 0.0 6 2 201 2 0.0 6 2 202 9 0.0 6 9 203 5 0.0 6 5 204 3 0.0 6 3 205 3 0.0 6 3 206 1 0.0 6 1 207 4 0.0 6 4 208 6 0.0 6 6 210 8 0.0 6 8 211 1 0.0 6 1 212 1 0.0 6 1 213 1 0.0 6 1 214 3 0.0 6 3 215 3 0.0 6 3 216 1 0.0 6 1 217 2 0.0 6 2 218 2 0.0 6 2 219 1 0.0 6 1 220 5 0.0 6 5 221 1 0.0 6 1 222 1 0.0 6 1 223 3 0.0 6 3 224 4 0.0 6 4 226 2 0.0 6 2 227 2 0.0 6 2 228 1 0.0 6 1 229 1 0.0 6 1 230 1 0.0 6 1 231 1 0.0 6 1 232 1 0.0 6 1 234 1 0.0 6 1 236 4 0.0 6 4 238 1 0.0 6 1 239 1 0.0 6 1 240 2 0.0 6 2 241 1 0.0 6 1 242 2 0.0 6 2 243 1 0.0 6 1 244 1 0.0 6 1 245 1 0.0 6 1 247 8 0.0 6 8 249 2 0.0 6 2 250 2 0.0 6 2 251 2 0.0 6 2 252 3 0.0 6 3 256 1 0.0 6 1 257 1 0.0 6 1 258 1 0.0 6 1 259 2 0.0 6 2 260 2 0.0 6 2 261 2 0.0 6 2 262 1 0.0 6 1 263 1 0.0 6 1 270 2 0.0 6 2 273 1 0.0 6 1 278 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 33963 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 540.8 0 0 1 11 2 0.0 3 1 1 12 3 0.0 3 3 13 1 0.0 3 1 14 6 0.0 4 6 15 3 0.0 4 3 16 3 0.0 4 2 0 1 17 18 0.0 5 15 3 18 8 0.0 5 8 19 12 0.0 5 10 2 20 21 0.0 6 20 1 21 23 0.0 6 23 22 16 0.0 6 15 1 23 11 0.0 6 11 24 10 0.0 6 10 25 7 0.0 6 7 26 8 0.0 6 8 27 6 0.0 6 5 1 28 11 0.0 6 10 1 29 10 0.0 6 9 1 30 6 0.0 6 6 31 2 0.0 6 2 32 3 0.0 6 3 33 5 0.0 6 5 34 5 0.0 6 4 1 35 7 0.0 6 7 36 5 0.0 6 5 37 4 0.0 6 3 1 38 7 0.0 6 6 1 39 6 0.0 6 6 40 6 0.0 6 5 1 41 7 0.0 6 7 42 5 0.0 6 5 43 3 0.0 6 2 0 0 1 44 5 0.0 6 5 45 3 0.0 6 3 46 11 0.0 6 11 47 4 0.0 6 4 48 4 0.0 6 4 49 8 0.0 6 8 50 9 0.0 6 7 2 51 6 0.0 6 6 52 9 0.0 6 9 53 8 0.0 6 7 1 54 12 0.0 6 11 0 1 55 22 0.0 6 20 2 56 7 0.0 6 6 1 57 16 0.0 6 16 58 15 0.0 6 15 59 17 0.0 6 15 1 0 1 60 11 0.0 6 10 0 1 61 11 0.0 6 8 3 62 11 0.0 6 9 1 1 63 7 0.0 6 7 64 11 0.0 6 10 1 65 12 0.0 6 8 4 66 7 0.0 6 6 1 67 20 0.0 6 19 1 68 51 0.0 6 49 1 0 1 69 23 0.0 6 21 2 70 25 0.0 6 24 1 71 20 0.0 6 18 2 72 22 0.0 6 21 1 73 22 0.0 6 20 2 74 10 0.0 6 10 75 13 0.0 6 13 76 14 0.0 6 14 77 18 0.0 6 17 1 78 17 0.0 6 16 1 79 12 0.0 6 11 1 80 26 0.0 6 26 81 24 0.0 6 23 1 82 15 0.0 6 14 1 83 25 0.0 6 23 1 1 84 40 0.0 6 39 1 85 22 0.0 6 19 2 0 0 0 0 1 86 14 0.0 6 14 87 25 0.0 6 25 88 21 0.0 6 21 89 17 0.0 6 16 1 90 26 0.0 6 26 91 28 0.0 6 25 2 1 92 24 0.0 6 21 3 93 27 0.0 6 26 1 94 47 0.0 6 47 95 33 0.0 6 33 96 37 0.0 6 36 1 97 39 0.0 6 39 98 28 0.0 6 27 1 99 51 0.0 6 49 2 100 28 0.0 6 28 101 52 0.0 6 51 1 102 36 0.0 6 34 2 103 39 0.0 6 37 2 104 54 0.0 6 54 105 77 0.0 6 74 2 0 0 0 0 1 106 41 0.0 6 39 0 1 0 1 107 46 0.0 6 46 108 41 0.0 6 40 1 109 46 0.0 6 44 2 110 45 0.0 6 43 2 111 60 0.0 6 58 2 112 65 0.0 6 61 4 113 62 0.0 6 58 1 1 0 0 0 2 114 54 0.0 6 52 2 115 54 0.0 6 52 1 1 116 75 0.0 6 72 2 0 0 1 117 71 0.0 6 69 1 0 0 0 0 1 118 66 0.0 6 65 1 119 74 0.0 6 73 0 0 0 0 0 1 120 78 0.0 6 75 3 121 79 0.0 6 77 2 122 70 0.0 6 67 2 1 123 88 0.0 6 87 1 124 99 0.0 6 97 2 125 89 0.0 6 88 0 1 126 124 0.0 6 121 3 127 140 0.0 6 135 4 0 0 0 0 1 128 126 0.0 6 119 6 1 129 148 0.0 6 145 1 1 0 1 130 140 0.0 6 135 5 131 172 0.0 6 167 5 132 182 0.0 6 179 3 133 171 0.0 6 169 2 134 176 0.0 6 171 5 135 229 0.0 6 221 7 0 1 136 207 0.0 6 205 2 137 223 0.0 6 222 1 138 216 0.0 6 213 3 139 311 0.0 6 308 3 140 341 0.0 6 335 5 1 141 351 0.0 6 344 6 0 1 142 440 0.0 6 435 5 143 449 0.0 6 448 1 144 402 0.0 6 400 2 145 463 0.0 6 461 2 146 471 0.0 6 467 4 147 539 0.0 6 531 7 0 1 148 574 0.0 6 570 2 1 0 1 149 644 0.0 6 638 4 0 1 0 0 1 150 560 0.0 6 551 7 2 151 677 0.0 6 663 9 1 0 2 1 1 152 604 0.0 6 594 8 0 1 0 1 153 585 0.0 6 574 10 0 0 0 1 154 641 0.0 6 631 8 0 1 0 1 155 489 0.0 6 480 9 156 516 0.0 6 506 8 0 0 1 0 1 157 464 0.0 6 457 7 158 538 0.0 6 532 5 0 1 159 512 0.0 6 502 8 0 0 0 1 1 160 469 0.0 6 465 4 161 447 0.0 6 443 3 0 0 0 0 1 162 431 0.0 6 426 3 0 0 1 0 1 163 436 0.0 6 430 5 0 1 164 442 0.0 6 433 8 0 0 0 0 1 165 433 0.0 6 419 13 1 166 424 0.0 6 413 8 2 0 0 0 1 167 483 0.0 6 477 2 1 2 0 1 168 406 0.0 6 402 2 1 0 0 1 169 475 0.0 6 470 5 170 372 0.0 6 371 1 171 438 0.0 6 434 4 172 437 0.0 6 434 3 173 406 0.0 6 400 6 174 490 0.0 6 486 4 175 480 0.0 6 473 5 0 2 176 453 0.0 6 444 7 0 0 0 0 2 177 526 0.0 6 516 8 0 1 1 178 386 0.0 6 383 3 179 376 0.0 6 374 2 180 325 0.0 6 318 7 181 297 0.0 6 292 5 182 266 0.0 6 264 2 183 322 0.0 6 320 2 184 340 0.0 6 336 4 185 428 0.0 6 425 3 186 523 0.0 6 514 8 0 0 1 187 328 0.0 6 324 3 1 188 309 0.0 6 306 3 189 287 0.0 6 284 3 190 382 0.0 6 374 8 191 361 0.0 6 348 12 0 1 192 326 0.0 6 321 5 193 210 0.0 6 208 2 194 254 0.0 6 246 8 195 250 0.0 6 247 2 0 0 0 1 196 466 0.0 6 463 3 197 254 0.0 6 249 4 0 0 1 198 296 0.0 6 287 9 199 398 0.0 6 391 7 200 216 0.0 6 211 4 0 0 0 1 201 218 0.0 6 216 2 202 184 0.0 6 177 7 203 128 0.0 6 123 4 1 204 136 0.0 6 133 3 205 84 0.0 6 82 2 206 70 0.0 6 70 207 81 0.0 6 81 208 107 0.0 6 105 2 209 78 0.0 6 77 0 0 0 1 210 73 0.0 6 72 1 211 76 0.0 6 75 1 212 96 0.0 6 90 5 0 0 0 1 213 50 0.0 6 50 214 75 0.0 6 73 2 215 98 0.0 6 97 1 216 64 0.0 6 63 1 217 79 0.0 6 76 2 0 0 1 218 71 0.0 6 68 3 219 77 0.0 6 74 3 220 29 0.0 6 29 221 46 0.0 6 45 1 222 60 0.0 6 60 223 52 0.0 6 52 224 33 0.0 6 31 2 225 47 0.0 6 47 226 33 0.0 6 32 1 227 29 0.0 6 29 228 33 0.0 6 31 2 229 45 0.0 6 44 1 230 30 0.0 6 30 231 20 0.0 6 20 232 34 0.0 6 34 233 52 0.0 6 52 234 14 0.0 6 13 1 235 13 0.0 6 13 236 9 0.0 6 9 237 4 0.0 6 4 238 4 0.0 6 4 239 4 0.0 6 4 240 10 0.0 6 10 241 9 0.0 6 9 242 31 0.0 6 30 1 243 18 0.0 6 17 1 244 6 0.0 6 6 245 3 0.0 6 3 246 9 0.0 6 9 248 2 0.0 6 2 249 1 0.0 6 1 250 2 0.0 6 2 251 1 0.0 6 1 252 3 0.0 6 3 253 4 0.0 6 4 254 1 0.0 6 1 255 1 0.0 6 1 277 1 0.0 6 1 279 5 0.0 6 5 280 45 0.0 6 43 1 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 624 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 58 1 0.0 6 1 70 1 0.0 6 1 71 5 0.0 6 5 72 7 0.0 6 7 73 4 0.0 6 4 74 4 0.0 6 4 75 7 0.0 6 7 76 7 0.0 6 7 77 8 0.0 6 8 78 8 0.0 6 8 79 11 0.0 6 11 80 8 0.0 6 8 81 7 0.0 6 7 82 3 0.0 6 3 83 4 0.0 6 4 84 2 0.0 6 2 85 6 0.0 6 6 86 6 0.0 6 6 87 5 0.0 6 5 88 5 0.0 6 5 89 5 0.0 6 5 90 5 0.0 6 5 91 8 0.0 6 8 92 5 0.0 6 5 93 6 0.0 6 6 94 3 0.0 6 3 95 3 0.0 6 3 96 5 0.0 6 5 97 7 0.0 6 7 98 5 0.0 6 5 99 8 0.0 6 8 100 9 0.0 6 9 101 3 0.0 6 3 102 1 0.0 6 1 103 9 0.0 6 9 104 2 0.0 6 2 105 2 0.0 6 2 106 8 0.0 6 8 107 2 0.0 6 1 1 108 8 0.0 6 8 109 8 0.0 6 8 110 5 0.0 6 5 111 2 0.0 6 2 112 8 0.0 6 8 113 6 0.0 6 6 114 2 0.0 6 2 116 5 0.0 6 5 117 1 0.0 6 1 118 2 0.0 6 2 119 4 0.0 6 4 120 4 0.0 6 4 121 1 0.0 6 1 122 4 0.0 6 4 123 3 0.0 6 3 124 3 0.0 6 3 125 1 0.0 6 1 126 2 0.0 6 2 127 7 0.0 6 6 0 0 0 1 128 8 0.0 6 8 129 2 0.0 6 2 130 1 0.0 6 1 131 35 0.0 6 35 132 7 0.0 6 7 133 6 0.0 6 6 134 1 0.0 6 1 135 8 0.0 6 8 136 3 0.0 6 3 137 3 0.0 6 3 138 4 0.0 6 4 139 2 0.0 6 2 140 5 0.0 6 5 141 5 0.0 6 5 142 5 0.0 6 5 143 4 0.0 6 4 144 2 0.0 6 2 145 4 0.0 6 4 146 2 0.0 6 2 147 10 0.0 6 10 148 3 0.0 6 3 149 4 0.0 6 4 150 2 0.0 6 2 151 4 0.0 6 4 152 16 0.0 6 16 154 4 0.0 6 4 156 4 0.0 6 4 157 8 0.0 6 8 158 5 0.0 6 5 159 2 0.0 6 2 160 4 0.0 6 4 161 1 0.0 6 1 162 2 0.0 6 2 163 5 0.0 6 5 164 1 0.0 6 1 165 2 0.0 6 2 166 22 0.0 6 22 167 7 0.0 6 7 168 11 0.0 6 11 169 8 0.0 6 7 1 170 8 0.0 6 8 171 3 0.0 6 3 172 4 0.0 6 4 173 5 0.0 6 5 174 1 0.0 6 1 175 6 0.0 6 6 176 3 0.0 6 3 177 4 0.0 6 4 178 2 0.0 6 2 179 5 0.0 6 5 180 2 0.0 6 2 181 4 0.0 6 4 182 2 0.0 6 2 183 3 0.0 6 3 184 4 0.0 6 4 185 1 0.0 6 1 186 3 0.0 6 3 187 1 0.0 6 1 188 1 0.0 6 1 189 2 0.0 6 2 190 5 0.0 6 5 192 2 0.0 6 2 193 3 0.0 6 3 194 3 0.0 6 3 195 2 0.0 6 2 196 1 0.0 6 1 197 1 0.0 6 1 198 1 0.0 6 1 199 1 0.0 6 1 201 2 0.0 6 2 202 1 0.0 6 1 204 3 0.0 6 3 205 1 0.0 6 1 206 2 0.0 6 2 207 1 0.0 6 1 209 2 0.0 6 1 0 1 210 1 0.0 6 1 211 1 0.0 6 1 212 2 0.0 6 2 213 1 0.0 6 1 215 2 0.0 6 2 217 1 0.0 6 1 218 1 0.0 6 1 219 1 0.0 6 1 221 1 0.0 6 1 238 1 0.0 6 1 239 1 0.0 6 1 241 1 0.0 6 1 244 1 0.0 6 1 246 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_13_177_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_13_404_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_13_177_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_13_404_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.40 s (166 µs/read; 0.36 M reads/minute). === Summary === Total read pairs processed: 26,400 Read 1 with adapter: 26,399 (100.0%) Read 2 with adapter: 26,393 (100.0%) == Read fate breakdown == Pairs that were too short: 19 (0.1%) Pairs discarded as untrimmed: 8 (0.0%) Pairs written (passing filters): 26,373 (99.9%) Total basepairs processed: 11,187,073 bp Read 1: 5,964,857 bp Read 2: 5,222,216 bp Total written (filtered): 1,689,938 bp (15.1%) Read 1: 861,329 bp Read 2: 828,609 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 25563 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 412.5 0 0 1 6 2 6.4 1 0 0 2 16 1 0.0 4 0 1 17 1 0.0 5 1 19 2 0.0 5 1 1 20 21 0.0 6 20 1 21 12 0.0 6 12 22 12 0.0 6 11 1 23 13 0.0 6 13 24 3 0.0 6 3 25 5 0.0 6 4 1 26 2 0.0 6 1 1 27 6 0.0 6 5 1 28 9 0.0 6 9 29 3 0.0 6 1 2 31 6 0.0 6 6 32 2 0.0 6 2 33 4 0.0 6 4 34 3 0.0 6 2 1 35 5 0.0 6 5 36 5 0.0 6 3 2 37 1 0.0 6 1 38 2 0.0 6 2 39 2 0.0 6 2 40 4 0.0 6 4 41 1 0.0 6 1 42 1 0.0 6 0 1 43 1 0.0 6 1 44 3 0.0 6 3 46 2 0.0 6 2 47 3 0.0 6 3 48 2 0.0 6 2 50 1 0.0 6 0 1 51 5 0.0 6 5 52 4 0.0 6 4 53 3 0.0 6 3 54 7 0.0 6 7 55 2 0.0 6 1 1 56 4 0.0 6 4 57 2 0.0 6 2 58 2 0.0 6 2 59 1 0.0 6 1 61 3 0.0 6 3 62 3 0.0 6 3 63 4 0.0 6 3 1 64 4 0.0 6 4 65 1 0.0 6 0 0 0 0 0 0 1 66 2 0.0 6 2 67 2 0.0 6 2 68 1 0.0 6 1 69 2 0.0 6 2 70 6 0.0 6 6 71 4 0.0 6 4 72 6 0.0 6 5 1 73 3 0.0 6 3 74 1 0.0 6 1 75 3 0.0 6 3 76 2 0.0 6 2 77 3 0.0 6 2 1 78 2 0.0 6 1 0 0 0 1 79 3 0.0 6 3 80 7 0.0 6 7 81 7 0.0 6 7 82 3 0.0 6 3 83 6 0.0 6 6 84 12 0.0 6 11 1 85 7 0.0 6 7 86 9 0.0 6 8 1 87 5 0.0 6 5 88 6 0.0 6 5 1 89 2 0.0 6 2 90 7 0.0 6 7 91 1 0.0 6 1 93 5 0.0 6 4 1 94 4 0.0 6 4 95 3 0.0 6 3 96 6 0.0 6 6 97 6 0.0 6 6 98 6 0.0 6 6 99 9 0.0 6 8 0 0 0 1 100 7 0.0 6 7 101 9 0.0 6 9 102 5 0.0 6 5 103 10 0.0 6 9 1 104 5 0.0 6 5 105 18 0.0 6 17 1 106 16 0.0 6 16 107 11 0.0 6 11 108 9 0.0 6 9 109 8 0.0 6 7 1 110 8 0.0 6 7 1 111 20 0.0 6 20 112 10 0.0 6 9 1 113 7 0.0 6 7 114 14 0.0 6 14 115 19 0.0 6 19 116 7 0.0 6 7 117 10 0.0 6 10 118 6 0.0 6 6 119 11 0.0 6 11 120 9 0.0 6 9 121 11 0.0 6 9 1 0 0 0 1 122 16 0.0 6 15 0 1 123 14 0.0 6 14 124 13 0.0 6 13 125 10 0.0 6 10 126 18 0.0 6 18 127 14 0.0 6 14 128 17 0.0 6 16 1 129 16 0.0 6 16 130 20 0.0 6 19 1 131 28 0.0 6 27 1 132 21 0.0 6 21 133 28 0.0 6 28 134 26 0.0 6 26 135 21 0.0 6 21 136 25 0.0 6 25 137 45 0.0 6 42 3 138 21 0.0 6 20 1 139 41 0.0 6 40 1 140 72 0.0 6 71 1 141 624 0.0 6 623 1 142 189 0.0 6 189 143 516 0.0 6 515 1 144 474 0.0 6 473 1 145 128 0.0 6 126 2 146 103 0.0 6 103 147 247 0.0 6 247 148 532 0.0 6 532 149 301 0.0 6 299 1 1 150 93 0.0 6 93 151 224 0.0 6 222 1 0 0 1 152 85 0.0 6 85 153 214 0.0 6 212 2 154 241 0.0 6 240 1 155 97 0.0 6 97 156 78 0.0 6 77 1 157 118 0.0 6 116 2 158 99 0.0 6 97 2 159 385 0.0 6 382 2 1 160 126 0.0 6 126 161 78 0.0 6 77 1 162 103 0.0 6 103 163 276 0.0 6 272 3 0 0 0 0 1 164 548 0.0 6 547 1 165 225 0.0 6 224 1 166 70 0.0 6 70 167 124 0.0 6 124 168 141 0.0 6 141 169 650 0.0 6 649 0 0 0 0 1 170 1215 0.0 6 1214 0 0 0 0 1 171 200 0.0 6 199 1 172 122 0.0 6 120 1 1 173 463 0.0 6 462 1 174 167 0.0 6 166 1 175 351 0.0 6 349 2 176 129 0.0 6 127 2 177 175 0.0 6 174 0 0 0 1 178 219 0.0 6 217 1 1 179 1323 0.0 6 1320 3 180 247 0.0 6 246 1 181 94 0.0 6 94 182 148 0.0 6 146 2 183 1314 0.0 6 1309 5 184 213 0.0 6 210 3 185 26 0.0 6 25 1 186 16 0.0 6 16 187 52 0.0 6 50 1 0 0 1 188 23 0.0 6 23 189 36 0.0 6 34 1 0 0 1 190 25 0.0 6 25 191 71 0.0 6 71 192 63 0.0 6 61 1 0 0 1 193 52 0.0 6 52 194 116 0.0 6 115 1 195 100 0.0 6 99 1 196 64 0.0 6 63 1 197 94 0.0 6 94 198 112 0.0 6 110 2 199 115 0.0 6 114 1 200 80 0.0 6 76 4 201 108 0.0 6 107 1 202 126 0.0 6 124 1 0 0 0 0 1 203 54 0.0 6 51 3 204 43 0.0 6 43 205 86 0.0 6 84 2 206 101 0.0 6 99 1 1 207 110 0.0 6 109 1 208 116 0.0 6 113 3 209 112 0.0 6 109 2 1 210 96 0.0 6 94 1 1 211 102 0.0 6 99 3 212 74 0.0 6 74 213 92 0.0 6 88 4 214 152 0.0 6 149 3 215 121 0.0 6 118 3 216 162 0.0 6 160 1 0 0 1 217 90 0.0 6 89 1 218 148 0.0 6 147 1 219 175 0.0 6 173 2 220 157 0.0 6 156 1 221 119 0.0 6 119 222 135 0.0 6 135 223 90 0.0 6 89 1 224 117 0.0 6 117 225 79 0.0 6 79 226 103 0.0 6 102 1 227 123 0.0 6 122 1 228 83 0.0 6 83 229 79 0.0 6 78 1 230 119 0.0 6 119 231 121 0.0 6 120 1 232 95 0.0 6 94 1 233 140 0.0 6 140 234 187 0.0 6 187 235 90 0.0 6 89 1 236 70 0.0 6 70 237 67 0.0 6 67 238 48 0.0 6 47 1 239 79 0.0 6 77 2 240 85 0.0 6 84 1 241 75 0.0 6 74 1 242 69 0.0 6 67 2 243 92 0.0 6 91 1 244 135 0.0 6 131 3 0 0 1 245 94 0.0 6 94 246 83 0.0 6 82 1 247 129 0.0 6 129 248 105 0.0 6 105 249 124 0.0 6 124 250 116 0.0 6 115 1 251 220 0.0 6 218 2 252 218 0.0 6 216 2 253 137 0.0 6 135 2 254 132 0.0 6 130 2 255 111 0.0 6 109 2 256 98 0.0 6 95 3 257 123 0.0 6 122 1 258 113 0.0 6 110 3 259 149 0.0 6 149 260 95 0.0 6 92 2 1 261 122 0.0 6 120 2 262 81 0.0 6 79 1 1 263 179 0.0 6 177 1 1 264 71 0.0 6 69 1 1 265 58 0.0 6 54 4 266 58 0.0 6 56 2 267 78 0.0 6 76 2 268 87 0.0 6 84 3 269 63 0.0 6 62 1 270 111 0.0 6 108 3 271 83 0.0 6 81 2 272 58 0.0 6 57 1 273 77 0.0 6 75 2 274 196 0.0 6 196 275 128 0.0 6 127 1 276 238 0.0 6 236 2 277 278 0.0 6 274 4 278 197 0.0 6 194 3 279 878 0.0 6 854 23 1 280 296 0.0 6 291 5 281 3 0.0 6 3 282 2 0.0 6 2 284 1 0.0 6 1 286 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 836 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 23 0.0 6 23 70 10 0.0 6 10 71 27 0.0 6 27 72 7 0.0 6 7 73 28 0.0 6 28 74 13 0.0 6 13 75 1 0.0 6 1 76 5 0.0 6 5 77 14 0.0 6 14 78 23 0.0 6 23 79 10 0.0 6 10 80 1 0.0 6 1 81 8 0.0 6 8 82 5 0.0 6 5 83 6 0.0 6 6 84 15 0.0 6 15 85 2 0.0 6 2 87 3 0.0 6 3 88 6 0.0 6 6 89 14 0.0 6 14 92 4 0.0 6 4 93 7 0.0 6 7 94 30 0.0 6 30 95 5 0.0 6 5 97 5 0.0 6 5 98 5 0.0 6 5 99 34 0.0 6 34 100 52 0.0 6 52 102 7 0.0 6 7 103 12 0.0 6 12 104 6 0.0 6 6 105 7 0.0 6 7 106 2 0.0 6 2 107 5 0.0 6 5 108 18 0.0 6 18 109 59 0.0 6 59 110 2 0.0 6 2 111 3 0.0 6 3 112 18 0.0 6 18 113 66 0.0 6 65 1 114 2 0.0 6 2 118 1 0.0 6 1 119 1 0.0 6 1 123 2 0.0 6 2 124 4 0.0 6 4 125 1 0.0 6 1 131 2 0.0 6 2 132 5 0.0 6 5 135 1 0.0 6 1 138 1 0.0 6 1 153 1 0.0 6 1 157 3 0.0 6 3 158 1 0.0 6 1 160 1 0.0 6 1 161 3 0.0 6 3 162 2 0.0 6 2 163 6 0.0 6 6 164 15 0.0 6 15 170 1 0.0 6 1 171 5 0.0 6 5 172 1 0.0 6 1 173 1 0.0 6 1 174 2 0.0 6 2 182 1 0.0 6 1 183 6 0.0 6 6 184 4 0.0 6 4 185 4 0.0 6 4 206 1 0.0 6 1 210 1 0.0 6 1 213 1 0.0 6 1 214 2 0.0 6 2 215 10 0.0 6 10 216 2 0.0 6 2 218 2 0.0 6 2 220 1 0.0 6 1 221 11 0.0 6 11 222 28 0.0 6 28 223 1 0.0 6 0 1 237 1 0.0 6 1 239 1 0.0 6 1 240 3 0.0 6 3 244 2 0.0 6 2 245 3 0.0 6 3 246 2 0.0 6 1 1 247 1 0.0 6 1 248 25 0.0 6 25 249 68 0.0 6 68 250 4 0.0 6 4 262 1 0.0 6 1 263 6 0.0 6 6 265 1 0.0 6 1 266 2 0.0 6 2 277 3 0.0 6 3 300 5 0.0 6 5 304 1 0.0 6 1 305 1 0.0 6 1 306 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 25616 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 11 1 0.0 3 1 12 3 0.0 3 3 13 4 0.0 3 3 1 14 1 0.0 4 1 15 1 0.0 4 1 17 2 0.0 5 2 18 1 0.0 5 1 19 3 0.0 5 1 2 20 12 0.0 6 12 21 4 0.0 6 4 22 4 0.0 6 4 23 4 0.0 6 3 0 0 1 24 1 0.0 6 1 25 1 0.0 6 1 26 3 0.0 6 2 1 27 4 0.0 6 4 28 9 0.0 6 8 0 0 0 1 29 1 0.0 6 1 30 3 0.0 6 3 31 2 0.0 6 2 32 2 0.0 6 2 33 1 0.0 6 1 34 3 0.0 6 2 1 36 1 0.0 6 1 37 4 0.0 6 4 38 5 0.0 6 4 1 39 2 0.0 6 1 1 40 2 0.0 6 1 1 41 2 0.0 6 2 42 3 0.0 6 3 43 2 0.0 6 2 44 1 0.0 6 1 45 2 0.0 6 2 47 1 0.0 6 1 48 4 0.0 6 4 49 1 0.0 6 1 50 2 0.0 6 2 51 2 0.0 6 2 52 4 0.0 6 4 53 3 0.0 6 3 54 5 0.0 6 4 1 55 5 0.0 6 5 56 8 0.0 6 8 57 5 0.0 6 4 0 1 58 2 0.0 6 2 59 6 0.0 6 6 60 6 0.0 6 6 61 2 0.0 6 1 1 62 3 0.0 6 3 63 3 0.0 6 3 64 4 0.0 6 4 65 6 0.0 6 6 66 13 0.0 6 12 1 67 7 0.0 6 6 1 68 10 0.0 6 10 69 64 0.0 6 62 1 0 1 70 14 0.0 6 13 1 71 20 0.0 6 18 2 72 12 0.0 6 11 0 1 73 13 0.0 6 12 1 74 11 0.0 6 11 75 8 0.0 6 7 1 76 12 0.0 6 12 77 4 0.0 6 4 78 8 0.0 6 8 79 6 0.0 6 6 80 12 0.0 6 12 81 14 0.0 6 14 82 11 0.0 6 11 83 11 0.0 6 11 84 12 0.0 6 12 85 24 0.0 6 24 86 10 0.0 6 10 87 15 0.0 6 14 1 88 9 0.0 6 8 1 89 9 0.0 6 9 90 9 0.0 6 8 1 91 11 0.0 6 10 1 92 11 0.0 6 11 93 13 0.0 6 13 94 20 0.0 6 20 95 20 0.0 6 20 96 20 0.0 6 19 1 97 27 0.0 6 27 98 29 0.0 6 27 2 99 17 0.0 6 16 1 100 18 0.0 6 18 101 23 0.0 6 23 102 15 0.0 6 15 103 14 0.0 6 14 104 23 0.0 6 21 2 105 15 0.0 6 15 106 49 0.0 6 48 1 107 22 0.0 6 21 1 108 24 0.0 6 24 109 31 0.0 6 30 1 110 25 0.0 6 24 1 111 20 0.0 6 19 0 0 0 0 0 1 112 42 0.0 6 40 2 113 29 0.0 6 28 1 114 48 0.0 6 48 115 38 0.0 6 37 1 116 38 0.0 6 37 1 117 47 0.0 6 47 118 38 0.0 6 36 1 1 119 58 0.0 6 58 120 54 0.0 6 53 1 121 49 0.0 6 49 122 37 0.0 6 37 123 43 0.0 6 43 124 51 0.0 6 50 1 125 57 0.0 6 56 1 126 48 0.0 6 48 127 76 0.0 6 76 128 58 0.0 6 58 129 87 0.0 6 86 0 1 130 87 0.0 6 85 1 0 0 0 1 131 62 0.0 6 62 132 64 0.0 6 63 1 133 104 0.0 6 102 2 134 99 0.0 6 99 135 133 0.0 6 132 1 136 162 0.0 6 161 1 137 260 0.0 6 257 2 0 1 138 229 0.0 6 225 4 139 596 0.0 6 596 140 202 0.0 6 202 141 500 0.0 6 499 1 142 458 0.0 6 456 2 143 269 0.0 6 266 3 144 249 0.0 6 248 1 145 412 0.0 6 408 4 146 547 0.0 6 543 3 0 0 0 1 147 372 0.0 6 370 1 0 0 0 1 148 220 0.0 6 220 149 296 0.0 6 293 1 0 0 1 1 150 295 0.0 6 292 3 151 459 0.0 6 457 2 152 450 0.0 6 443 5 1 0 0 1 153 266 0.0 6 263 1 0 0 0 2 154 328 0.0 6 326 2 155 307 0.0 6 304 2 1 156 294 0.0 6 290 4 157 434 0.0 6 431 2 1 158 242 0.0 6 241 1 159 259 0.0 6 254 5 160 369 0.0 6 364 5 161 340 0.0 6 334 6 162 452 0.0 6 447 4 1 163 260 0.0 6 256 4 164 329 0.0 6 327 2 165 273 0.0 6 270 2 0 0 0 1 166 315 0.0 6 314 1 167 427 0.0 6 426 1 168 608 0.0 6 605 3 169 197 0.0 6 192 2 0 1 0 2 170 265 0.0 6 262 3 171 301 0.0 6 297 3 0 1 172 235 0.0 6 229 5 0 0 0 1 173 309 0.0 6 304 4 1 174 261 0.0 6 259 2 175 336 0.0 6 334 2 176 415 0.0 6 410 5 177 583 0.0 6 580 2 0 0 0 1 178 231 0.0 6 229 1 1 179 240 0.0 6 233 5 0 0 0 1 1 180 328 0.0 6 324 3 0 0 0 1 181 343 0.0 6 337 6 182 100 0.0 6 96 4 183 123 0.0 6 120 3 184 93 0.0 6 90 3 185 323 0.0 6 311 8 2 0 0 2 186 311 0.0 6 298 12 0 0 0 1 187 159 0.0 6 152 5 1 0 0 1 188 115 0.0 6 110 4 1 189 148 0.0 6 138 10 190 137 0.0 6 131 5 1 191 152 0.0 6 148 3 0 0 0 1 192 114 0.0 6 113 1 193 195 0.0 6 187 6 1 0 0 0 1 194 284 0.0 6 278 6 195 143 0.0 6 137 5 0 0 1 196 209 0.0 6 203 6 197 78 0.0 6 78 198 194 0.0 6 191 3 199 220 0.0 6 214 6 200 146 0.0 6 139 7 201 147 0.0 6 136 8 2 0 0 0 1 202 178 0.0 6 173 5 203 133 0.0 6 126 7 204 203 0.0 6 197 6 205 248 0.0 6 237 10 0 1 206 86 0.0 6 82 4 207 244 0.0 6 233 10 0 0 1 208 93 0.0 6 91 2 209 104 0.0 6 98 4 0 0 0 0 2 210 56 0.0 6 54 1 0 0 0 0 1 211 144 0.0 6 143 0 1 212 111 0.0 6 103 7 0 0 0 0 1 213 260 0.0 6 249 8 2 0 1 214 115 0.0 6 110 5 215 124 0.0 6 121 1 0 0 0 0 2 216 296 0.0 6 288 6 0 0 0 1 1 217 134 0.0 6 131 3 218 221 0.0 6 219 2 219 103 0.0 6 98 4 0 0 0 0 1 220 58 0.0 6 58 221 87 0.0 6 87 222 31 0.0 6 29 2 223 40 0.0 6 39 1 224 90 0.0 6 88 2 225 63 0.0 6 62 1 226 47 0.0 6 47 227 76 0.0 6 76 228 49 0.0 6 46 3 229 73 0.0 6 73 230 144 0.0 6 143 0 0 0 1 231 85 0.0 6 83 2 232 94 0.0 6 93 1 233 44 0.0 6 43 1 234 59 0.0 6 57 2 235 41 0.0 6 41 236 26 0.0 6 25 1 237 33 0.0 6 31 2 238 42 0.0 6 42 239 9 0.0 6 8 1 240 16 0.0 6 16 241 29 0.0 6 29 242 23 0.0 6 23 243 21 0.0 6 21 244 10 0.0 6 10 245 16 0.0 6 16 246 11 0.0 6 11 247 18 0.0 6 18 248 9 0.0 6 8 1 249 16 0.0 6 16 250 10 0.0 6 10 251 16 0.0 6 15 1 252 9 0.0 6 9 253 8 0.0 6 8 254 6 0.0 6 6 255 9 0.0 6 9 256 4 0.0 6 3 1 280 2 0.0 6 2 281 26 0.0 6 26 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 777 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 51 1 0.0 6 1 68 1 0.0 6 1 69 1 0.0 6 1 70 31 0.0 6 31 71 9 0.0 6 9 72 35 0.0 6 35 73 16 0.0 6 16 74 3 0.0 6 2 0 0 0 1 75 3 0.0 6 3 76 6 0.0 6 6 77 31 0.0 6 31 78 10 0.0 6 10 80 16 0.0 6 16 81 4 0.0 6 4 82 9 0.0 6 9 83 15 0.0 6 15 84 1 0.0 6 1 85 1 0.0 6 1 86 6 0.0 6 6 87 1 0.0 6 1 88 18 0.0 6 18 89 1 0.0 6 1 91 4 0.0 6 4 92 18 0.0 6 18 93 32 0.0 6 32 94 6 0.0 6 6 95 2 0.0 6 2 96 6 0.0 6 6 97 10 0.0 6 10 98 44 0.0 6 44 99 65 0.0 6 65 100 1 0.0 6 1 101 7 0.0 6 7 102 22 0.0 6 22 103 6 0.0 6 5 1 104 19 0.0 6 18 0 0 1 106 5 0.0 6 5 107 25 0.0 6 24 1 108 65 0.0 6 65 109 1 0.0 6 1 110 2 0.0 6 2 111 11 0.0 6 11 112 78 0.0 6 78 113 2 0.0 6 2 115 1 0.0 6 0 0 0 0 1 121 2 0.0 6 2 130 2 0.0 6 2 131 4 0.0 6 4 133 1 0.0 6 1 134 1 0.0 6 1 136 1 0.0 6 1 137 1 0.0 6 1 156 2 0.0 6 2 161 2 0.0 6 1 1 162 4 0.0 6 4 163 15 0.0 6 15 165 1 0.0 6 1 168 1 0.0 6 1 170 1 0.0 6 1 172 2 0.0 6 2 173 5 0.0 6 5 178 1 0.0 6 1 181 1 0.0 6 1 182 4 0.0 6 4 183 6 0.0 6 6 184 3 0.0 6 3 194 1 0.0 6 1 213 4 0.0 6 4 214 7 0.0 6 7 220 1 0.0 6 1 221 10 0.0 6 10 222 1 0.0 6 1 239 1 0.0 6 1 243 1 0.0 6 0 0 1 247 9 0.0 6 8 1 248 28 0.0 6 28 249 1 0.0 6 1 262 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_07_178_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_07_405_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_07_178_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_07_405_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 9.19 s (65 µs/read; 0.92 M reads/minute). === Summary === Total read pairs processed: 140,759 Read 1 with adapter: 140,727 (100.0%) Read 2 with adapter: 140,631 (99.9%) == Read fate breakdown == Pairs that were too short: 57 (0.0%) Pairs discarded as untrimmed: 160 (0.1%) Pairs written (passing filters): 140,542 (99.8%) Total basepairs processed: 59,175,460 bp Read 1: 32,922,180 bp Read 2: 26,253,280 bp Total written (filtered): 9,311,922 bp (15.7%) Read 1: 4,719,325 bp Read 2: 4,592,597 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 136494 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 2199.4 0 0 2 4 9 549.8 1 4 5 5 3 137.5 1 3 6 6 34.4 1 2 0 4 7 5 8.6 2 4 0 1 9 3 0.5 2 3 11 2 0.0 3 2 12 2 0.0 3 2 13 1 0.0 3 1 15 2 0.0 4 2 16 2 0.0 4 1 1 17 14 0.0 5 14 18 1 0.0 5 1 19 8 0.0 5 7 1 20 255 0.0 6 253 2 21 139 0.0 6 134 5 22 107 0.0 6 101 6 23 74 0.0 6 72 2 24 50 0.0 6 49 1 25 40 0.0 6 39 1 26 55 0.0 6 51 3 0 1 27 23 0.0 6 22 1 28 25 0.0 6 21 3 0 0 0 1 29 27 0.0 6 24 3 30 12 0.0 6 12 31 22 0.0 6 21 0 0 0 0 0 1 32 14 0.0 6 13 1 33 7 0.0 6 7 34 19 0.0 6 19 35 16 0.0 6 14 2 36 9 0.0 6 8 0 0 1 37 5 0.0 6 5 38 16 0.0 6 13 3 39 21 0.0 6 21 40 19 0.0 6 19 41 30 0.0 6 28 2 42 20 0.0 6 19 1 43 15 0.0 6 15 44 16 0.0 6 14 2 45 15 0.0 6 15 46 11 0.0 6 10 1 47 11 0.0 6 10 1 48 21 0.0 6 21 49 15 0.0 6 15 50 21 0.0 6 18 3 51 15 0.0 6 13 2 52 17 0.0 6 16 1 53 6 0.0 6 5 1 54 9 0.0 6 9 55 9 0.0 6 9 56 8 0.0 6 8 57 8 0.0 6 7 1 58 11 0.0 6 10 0 1 59 17 0.0 6 16 1 60 10 0.0 6 10 61 5 0.0 6 4 1 62 9 0.0 6 8 1 63 12 0.0 6 12 64 14 0.0 6 14 65 19 0.0 6 18 1 66 16 0.0 6 15 0 0 0 0 1 67 14 0.0 6 13 0 0 1 68 9 0.0 6 8 1 69 12 0.0 6 12 70 24 0.0 6 23 1 71 21 0.0 6 20 0 1 72 14 0.0 6 14 73 27 0.0 6 25 2 74 22 0.0 6 20 2 75 22 0.0 6 22 76 10 0.0 6 10 77 21 0.0 6 19 2 78 14 0.0 6 13 1 79 12 0.0 6 12 80 17 0.0 6 16 1 81 18 0.0 6 17 1 82 13 0.0 6 13 83 38 0.0 6 36 2 84 17 0.0 6 16 1 85 27 0.0 6 26 1 86 28 0.0 6 28 87 23 0.0 6 22 1 88 18 0.0 6 18 89 15 0.0 6 15 90 19 0.0 6 17 1 1 91 15 0.0 6 12 2 0 0 0 0 1 92 17 0.0 6 16 0 0 0 0 0 1 93 17 0.0 6 16 0 0 0 0 1 94 20 0.0 6 18 1 0 0 0 0 1 95 27 0.0 6 25 2 96 11 0.0 6 11 97 15 0.0 6 14 1 98 25 0.0 6 24 0 1 99 20 0.0 6 19 1 100 25 0.0 6 25 101 22 0.0 6 21 1 102 34 0.0 6 33 1 103 30 0.0 6 29 1 104 37 0.0 6 37 105 34 0.0 6 32 0 1 0 0 0 1 106 25 0.0 6 25 107 30 0.0 6 28 1 1 108 23 0.0 6 22 1 109 29 0.0 6 26 2 0 0 0 0 1 110 34 0.0 6 32 1 0 0 0 1 111 48 0.0 6 45 1 2 112 47 0.0 6 47 113 24 0.0 6 22 1 1 114 48 0.0 6 48 115 46 0.0 6 45 0 0 0 0 1 116 41 0.0 6 39 1 1 117 47 0.0 6 46 0 0 0 0 1 118 41 0.0 6 40 1 119 53 0.0 6 53 120 44 0.0 6 42 2 121 37 0.0 6 33 1 0 3 122 48 0.0 6 47 1 123 49 0.0 6 47 2 124 64 0.0 6 62 1 0 0 0 0 1 125 59 0.0 6 58 1 126 59 0.0 6 59 127 55 0.0 6 54 1 128 64 0.0 6 64 129 69 0.0 6 68 1 130 89 0.0 6 88 1 131 70 0.0 6 69 0 1 132 58 0.0 6 57 0 0 1 133 67 0.0 6 67 134 82 0.0 6 79 2 1 135 91 0.0 6 88 3 136 71 0.0 6 70 1 137 84 0.0 6 84 138 94 0.0 6 94 139 110 0.0 6 109 1 140 108 0.0 6 105 3 141 135 0.0 6 133 2 142 164 0.0 6 161 2 0 0 0 1 143 409 0.0 6 408 1 144 794 0.0 6 793 1 145 884 0.0 6 882 2 146 874 0.0 6 870 4 147 616 0.0 6 615 1 148 960 0.0 6 957 3 149 906 0.0 6 903 3 150 1097 0.0 6 1094 3 151 875 0.0 6 874 1 152 692 0.0 6 691 0 0 0 0 1 153 699 0.0 6 696 3 154 708 0.0 6 706 1 0 0 0 1 155 575 0.0 6 571 4 156 555 0.0 6 554 1 157 626 0.0 6 626 158 607 0.0 6 606 1 159 549 0.0 6 545 3 0 0 0 0 1 160 792 0.0 6 787 5 161 635 0.0 6 631 3 0 0 1 162 824 0.0 6 821 3 163 734 0.0 6 730 4 164 777 0.0 6 774 3 165 774 0.0 6 770 3 0 0 0 1 166 846 0.0 6 842 3 0 0 0 0 1 167 714 0.0 6 711 2 0 0 0 0 1 168 728 0.0 6 725 2 0 1 169 802 0.0 6 800 1 0 0 0 1 170 906 0.0 6 902 4 171 870 0.0 6 869 1 172 867 0.0 6 866 0 0 0 1 173 872 0.0 6 868 3 0 0 1 174 852 0.0 6 850 2 175 938 0.0 6 934 4 176 905 0.0 6 894 10 1 177 768 0.0 6 766 2 178 846 0.0 6 846 179 778 0.0 6 774 4 180 956 0.0 6 950 6 181 2110 0.0 6 2108 1 0 0 0 1 182 1177 0.0 6 1172 5 183 944 0.0 6 941 3 184 875 0.0 6 872 3 185 898 0.0 6 897 1 186 927 0.0 6 921 5 0 0 0 0 1 187 960 0.0 6 958 2 188 1011 0.0 6 1008 3 189 942 0.0 6 936 4 0 0 1 0 1 190 911 0.0 6 905 5 1 191 982 0.0 6 977 4 0 0 1 192 1084 0.0 6 1077 5 0 0 0 0 2 193 1295 0.0 6 1289 6 194 1289 0.0 6 1280 9 195 1512 0.0 6 1502 9 0 0 0 1 196 1744 0.0 6 1738 5 1 197 1864 0.0 6 1858 5 0 0 0 1 198 1940 0.0 6 1930 10 199 1770 0.0 6 1762 8 200 1295 0.0 6 1287 7 0 0 1 201 1468 0.0 6 1457 9 0 0 1 0 1 202 1468 0.0 6 1462 6 203 1474 0.0 6 1464 8 1 1 204 1585 0.0 6 1574 10 0 0 0 1 205 2375 0.0 6 2368 6 0 1 206 2125 0.0 6 2118 4 0 0 1 2 207 1674 0.0 6 1661 11 1 0 0 0 1 208 1702 0.0 6 1695 6 0 1 209 1366 0.0 6 1359 7 210 1466 0.0 6 1459 7 211 1662 0.0 6 1656 6 212 1756 0.0 6 1748 8 213 1750 0.0 6 1741 9 214 2122 0.0 6 2111 9 1 1 215 2190 0.0 6 2180 10 216 1779 0.0 6 1770 6 0 1 0 1 1 217 1824 0.0 6 1819 5 218 1889 0.0 6 1881 5 0 0 0 1 2 219 1762 0.0 6 1756 6 220 1492 0.0 6 1482 8 0 0 0 2 221 1443 0.0 6 1438 5 222 1423 0.0 6 1421 2 223 1593 0.0 6 1584 8 0 1 224 1591 0.0 6 1586 4 0 0 1 225 1627 0.0 6 1622 5 226 1576 0.0 6 1571 4 1 227 1273 0.0 6 1269 4 228 1225 0.0 6 1221 3 1 229 1218 0.0 6 1217 1 230 1274 0.0 6 1265 8 0 0 0 0 1 231 1272 0.0 6 1265 5 1 0 0 0 1 232 1225 0.0 6 1219 5 1 233 1138 0.0 6 1132 4 1 0 0 0 1 234 1107 0.0 6 1104 3 235 930 0.0 6 925 5 236 952 0.0 6 948 3 1 237 894 0.0 6 887 7 238 1038 0.0 6 1033 5 239 914 0.0 6 909 4 1 240 842 0.0 6 839 3 241 843 0.0 6 839 4 242 772 0.0 6 768 4 243 743 0.0 6 739 2 2 244 667 0.0 6 665 1 0 0 0 0 1 245 600 0.0 6 599 1 246 547 0.0 6 542 5 247 528 0.0 6 523 4 0 0 0 1 248 487 0.0 6 485 0 0 1 1 249 506 0.0 6 504 1 0 0 0 1 250 494 0.0 6 489 5 251 442 0.0 6 440 1 0 1 252 411 0.0 6 408 3 253 404 0.0 6 401 3 254 354 0.0 6 352 1 0 0 0 1 255 344 0.0 6 344 256 360 0.0 6 359 1 257 374 0.0 6 373 1 258 329 0.0 6 327 2 259 377 0.0 6 376 1 260 311 0.0 6 310 0 0 0 1 261 224 0.0 6 223 1 262 260 0.0 6 260 263 214 0.0 6 214 264 226 0.0 6 224 1 0 0 0 1 265 214 0.0 6 214 266 203 0.0 6 202 1 267 176 0.0 6 175 1 268 198 0.0 6 198 269 216 0.0 6 213 2 0 1 270 177 0.0 6 177 271 165 0.0 6 165 272 151 0.0 6 149 2 273 168 0.0 6 166 2 274 215 0.0 6 214 1 275 241 0.0 6 240 0 0 1 276 312 0.0 6 309 2 1 277 444 0.0 6 443 1 278 1303 0.0 6 1301 2 279 2368 0.0 6 2349 14 4 0 0 1 280 15 0.0 6 15 281 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 4233 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 59 1 0.0 6 0 0 0 0 1 60 1 0.0 6 1 61 5 0.0 6 2 0 3 62 5 0.0 6 0 5 63 496 0.0 6 478 17 1 64 2 0.0 6 1 1 65 1 0.0 6 1 71 7 0.0 6 7 72 19 0.0 6 19 73 27 0.0 6 27 74 31 0.0 6 31 75 21 0.0 6 21 76 23 0.0 6 23 77 25 0.0 6 25 78 25 0.0 6 24 1 79 38 0.0 6 38 80 28 0.0 6 28 81 24 0.0 6 24 82 21 0.0 6 20 1 83 12 0.0 6 12 84 16 0.0 6 16 85 26 0.0 6 26 86 20 0.0 6 19 1 87 11 0.0 6 11 88 10 0.0 6 10 89 17 0.0 6 17 90 23 0.0 6 23 91 28 0.0 6 28 92 18 0.0 6 18 93 22 0.0 6 22 94 16 0.0 6 16 95 19 0.0 6 19 96 21 0.0 6 21 97 27 0.0 6 27 98 19 0.0 6 19 99 25 0.0 6 25 100 22 0.0 6 22 101 23 0.0 6 22 1 102 23 0.0 6 22 0 0 0 0 0 1 103 23 0.0 6 23 104 29 0.0 6 29 105 22 0.0 6 22 106 15 0.0 6 15 107 14 0.0 6 14 108 16 0.0 6 15 0 1 109 51 0.0 6 51 110 53 0.0 6 53 111 24 0.0 6 24 112 19 0.0 6 19 113 18 0.0 6 18 114 13 0.0 6 13 115 14 0.0 6 14 116 13 0.0 6 13 117 23 0.0 6 23 118 15 0.0 6 15 119 20 0.0 6 19 1 120 17 0.0 6 17 121 14 0.0 6 14 122 22 0.0 6 22 123 21 0.0 6 21 124 16 0.0 6 16 125 28 0.0 6 28 126 27 0.0 6 26 1 127 26 0.0 6 26 128 30 0.0 6 30 129 24 0.0 6 24 130 29 0.0 6 29 131 23 0.0 6 23 132 22 0.0 6 22 133 38 0.0 6 37 0 0 0 0 0 1 134 65 0.0 6 64 1 135 24 0.0 6 24 136 19 0.0 6 19 137 25 0.0 6 25 138 17 0.0 6 17 139 32 0.0 6 32 140 27 0.0 6 27 141 21 0.0 6 21 142 33 0.0 6 33 143 32 0.0 6 32 144 24 0.0 6 24 145 17 0.0 6 17 146 19 0.0 6 19 147 41 0.0 6 41 148 24 0.0 6 24 149 27 0.0 6 27 150 26 0.0 6 26 151 26 0.0 6 26 152 28 0.0 6 28 153 22 0.0 6 22 154 31 0.0 6 31 155 28 0.0 6 28 156 36 0.0 6 36 157 33 0.0 6 33 158 34 0.0 6 33 1 159 20 0.0 6 20 160 15 0.0 6 15 161 17 0.0 6 17 162 31 0.0 6 31 163 31 0.0 6 31 164 45 0.0 6 45 165 31 0.0 6 31 166 29 0.0 6 29 167 33 0.0 6 33 168 18 0.0 6 18 169 27 0.0 6 27 170 31 0.0 6 31 171 26 0.0 6 26 172 20 0.0 6 20 173 25 0.0 6 24 1 174 29 0.0 6 29 175 29 0.0 6 29 176 19 0.0 6 19 177 20 0.0 6 20 178 23 0.0 6 23 179 36 0.0 6 36 180 24 0.0 6 24 181 23 0.0 6 23 182 28 0.0 6 28 183 20 0.0 6 20 184 23 0.0 6 23 185 30 0.0 6 30 186 31 0.0 6 31 187 14 0.0 6 14 188 28 0.0 6 27 1 189 21 0.0 6 21 190 19 0.0 6 19 191 38 0.0 6 38 192 24 0.0 6 24 193 27 0.0 6 27 194 16 0.0 6 16 195 21 0.0 6 21 196 7 0.0 6 7 197 10 0.0 6 10 198 12 0.0 6 12 199 9 0.0 6 9 200 29 0.0 6 29 201 17 0.0 6 17 202 12 0.0 6 12 203 22 0.0 6 22 204 15 0.0 6 15 205 21 0.0 6 21 206 11 0.0 6 10 0 0 0 0 0 1 207 11 0.0 6 11 208 19 0.0 6 19 209 16 0.0 6 16 210 14 0.0 6 14 211 11 0.0 6 11 212 12 0.0 6 12 213 12 0.0 6 12 214 7 0.0 6 7 215 12 0.0 6 12 216 6 0.0 6 6 217 10 0.0 6 10 218 12 0.0 6 12 219 9 0.0 6 9 220 8 0.0 6 8 221 11 0.0 6 11 222 11 0.0 6 11 223 8 0.0 6 8 224 10 0.0 6 10 225 6 0.0 6 6 226 8 0.0 6 8 227 10 0.0 6 10 228 15 0.0 6 15 229 6 0.0 6 6 230 8 0.0 6 8 231 9 0.0 6 9 232 12 0.0 6 12 233 11 0.0 6 11 234 7 0.0 6 7 235 3 0.0 6 3 236 8 0.0 6 8 237 8 0.0 6 7 1 238 6 0.0 6 6 239 8 0.0 6 8 240 1 0.0 6 1 241 5 0.0 6 5 242 8 0.0 6 8 243 5 0.0 6 5 244 6 0.0 6 6 245 9 0.0 6 9 246 7 0.0 6 7 247 3 0.0 6 3 248 11 0.0 6 11 249 7 0.0 6 7 250 5 0.0 6 5 251 9 0.0 6 9 252 7 0.0 6 7 253 4 0.0 6 4 254 8 0.0 6 8 255 9 0.0 6 9 256 4 0.0 6 4 257 5 0.0 6 5 258 3 0.0 6 3 259 4 0.0 6 4 260 2 0.0 6 2 261 4 0.0 6 4 262 2 0.0 6 2 263 2 0.0 6 2 264 4 0.0 6 4 265 2 0.0 6 2 266 1 0.0 6 1 267 2 0.0 6 2 268 2 0.0 6 2 269 1 0.0 6 1 270 2 0.0 6 2 271 2 0.0 6 2 272 4 0.0 6 4 274 2 0.0 6 2 276 3 0.0 6 3 277 2 0.0 6 2 279 6 0.0 6 6 280 1 0.0 6 1 281 1 0.0 6 1 282 2 0.0 6 2 283 2 0.0 6 2 290 1 0.0 6 0 0 0 0 0 0 1 292 1 0.0 6 1 296 2 0.0 6 2 307 1 0.0 6 0 0 0 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 137182 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 2199.4 0 1 3 5 1 137.5 1 1 6 2 34.4 1 2 7 2 8.6 2 1 1 9 2 0.5 2 1 1 10 6 0.1 3 5 1 11 10 0.0 3 10 12 5 0.0 3 4 0 1 13 10 0.0 3 9 1 14 27 0.0 4 23 3 1 15 4 0.0 4 3 1 16 34 0.0 4 30 4 17 46 0.0 5 42 4 18 36 0.0 5 32 4 19 39 0.0 5 35 3 1 20 267 0.0 6 259 8 21 129 0.0 6 125 4 22 66 0.0 6 61 5 23 78 0.0 6 75 2 0 1 24 61 0.0 6 59 2 25 63 0.0 6 60 3 26 49 0.0 6 42 7 27 38 0.0 6 34 4 28 48 0.0 6 45 3 29 36 0.0 6 35 1 30 32 0.0 6 29 3 31 43 0.0 6 41 0 1 0 1 32 25 0.0 6 22 2 1 33 30 0.0 6 27 3 34 29 0.0 6 28 0 1 35 28 0.0 6 23 4 1 36 28 0.0 6 26 2 37 27 0.0 6 25 2 38 25 0.0 6 24 1 39 37 0.0 6 35 2 40 31 0.0 6 25 3 0 0 3 41 43 0.0 6 39 4 42 33 0.0 6 31 2 43 37 0.0 6 32 5 44 24 0.0 6 22 2 45 50 0.0 6 43 7 46 30 0.0 6 27 3 47 21 0.0 6 20 1 48 43 0.0 6 40 3 49 43 0.0 6 40 2 0 0 0 0 1 50 39 0.0 6 34 4 1 51 44 0.0 6 40 4 52 50 0.0 6 47 3 53 53 0.0 6 46 5 1 0 0 1 54 56 0.0 6 55 1 55 59 0.0 6 54 4 0 0 1 56 66 0.0 6 61 2 0 1 2 57 47 0.0 6 40 7 58 49 0.0 6 47 2 59 55 0.0 6 51 3 1 60 54 0.0 6 50 3 0 1 61 56 0.0 6 55 0 1 62 47 0.0 6 46 1 63 72 0.0 6 67 5 64 101 0.0 6 93 7 0 0 1 65 72 0.0 6 62 9 1 66 99 0.0 6 88 7 0 3 0 0 1 67 266 0.0 6 253 9 1 1 0 0 2 68 125 0.0 6 118 5 0 1 0 1 69 169 0.0 6 157 8 2 1 0 0 1 70 86 0.0 6 81 4 0 0 0 0 1 71 112 0.0 6 100 12 72 71 0.0 6 65 3 2 1 73 67 0.0 6 61 6 74 96 0.0 6 87 7 0 0 0 1 1 75 90 0.0 6 83 5 0 1 0 1 76 103 0.0 6 95 7 0 0 1 77 91 0.0 6 84 7 78 81 0.0 6 81 79 110 0.0 6 106 3 1 80 147 0.0 6 141 4 1 1 81 112 0.0 6 101 9 2 82 144 0.0 6 136 7 0 0 0 0 1 83 163 0.0 6 154 8 1 84 116 0.0 6 106 9 0 1 85 90 0.0 6 84 3 1 1 0 0 1 86 106 0.0 6 99 5 2 87 99 0.0 6 87 11 0 0 0 0 1 88 109 0.0 6 100 6 0 2 1 89 95 0.0 6 88 6 0 0 1 90 137 0.0 6 134 2 0 1 91 129 0.0 6 122 6 1 92 159 0.0 6 154 5 93 191 0.0 6 181 8 0 1 0 1 94 145 0.0 6 138 5 1 1 95 171 0.0 6 160 9 1 0 0 1 96 186 0.0 6 182 3 1 97 177 0.0 6 170 6 0 1 98 160 0.0 6 154 3 0 2 1 99 148 0.0 6 140 5 2 0 0 0 1 100 171 0.0 6 166 5 101 174 0.0 6 165 8 0 0 0 1 102 200 0.0 6 190 8 1 0 0 0 1 103 252 0.0 6 248 2 0 0 1 1 104 407 0.0 6 389 16 0 1 1 105 200 0.0 6 196 3 0 0 0 1 106 228 0.0 6 218 8 1 0 0 1 107 242 0.0 6 233 4 2 1 0 1 1 108 222 0.0 6 213 8 0 0 0 1 109 236 0.0 6 229 6 1 110 263 0.0 6 258 5 111 274 0.0 6 263 7 0 2 0 1 1 112 325 0.0 6 319 4 1 0 1 113 273 0.0 6 263 10 114 273 0.0 6 269 4 115 327 0.0 6 317 7 3 116 345 0.0 6 334 9 0 1 1 117 381 0.0 6 366 14 1 118 385 0.0 6 375 7 1 0 1 1 119 367 0.0 6 352 13 1 0 1 120 345 0.0 6 335 8 1 0 0 0 1 121 405 0.0 6 390 11 1 2 1 122 362 0.0 6 347 12 2 0 1 123 447 0.0 6 440 6 0 0 0 0 1 124 483 0.0 6 467 14 0 0 0 1 1 125 543 0.0 6 535 7 0 0 0 0 1 126 578 0.0 6 569 9 127 661 0.0 6 643 16 0 0 0 2 128 694 0.0 6 675 17 0 0 1 1 129 705 0.0 6 687 13 4 0 1 130 743 0.0 6 723 16 0 0 1 1 2 131 1088 0.0 6 1058 26 1 2 0 1 132 897 0.0 6 876 20 0 0 1 133 880 0.0 6 866 10 2 2 134 906 0.0 6 884 18 0 1 2 0 1 135 956 0.0 6 943 10 2 0 0 0 1 136 895 0.0 6 882 9 0 2 1 1 137 895 0.0 6 876 16 1 2 138 960 0.0 6 944 14 1 0 1 139 1120 0.0 6 1102 17 0 0 0 0 1 140 1477 0.0 6 1461 15 0 0 1 141 1851 0.0 6 1832 14 1 1 1 0 2 142 2040 0.0 6 2011 25 0 0 1 1 2 143 1896 0.0 6 1876 18 1 0 0 1 144 1817 0.0 6 1794 19 0 1 2 0 1 145 2200 0.0 6 2171 21 2 1 2 2 1 146 2283 0.0 6 2249 26 2 2 1 2 1 147 2378 0.0 6 2343 29 4 0 0 2 148 2625 0.0 6 2598 25 1 0 1 149 2516 0.0 6 2479 30 2 1 1 1 2 150 3353 0.0 6 3306 37 4 1 1 0 4 151 2763 0.0 6 2726 32 1 0 2 0 2 152 2971 0.0 6 2938 25 2 4 2 153 2950 0.0 6 2908 36 1 0 2 1 2 154 2437 0.0 6 2413 20 2 0 0 1 1 155 2573 0.0 6 2542 29 0 1 0 1 156 2369 0.0 6 2330 30 2 2 0 1 4 157 2626 0.0 6 2598 24 2 0 1 1 158 2592 0.0 6 2571 16 0 0 1 1 3 159 2285 0.0 6 2266 18 0 0 0 0 1 160 2367 0.0 6 2346 19 0 0 1 1 161 2183 0.0 6 2164 18 0 0 0 0 1 162 1912 0.0 6 1895 13 0 1 0 2 1 163 2014 0.0 6 1994 12 1 1 4 2 164 1923 0.0 6 1909 12 1 0 1 165 1886 0.0 6 1869 15 0 1 0 0 1 166 2328 0.0 6 2308 17 0 1 1 1 167 2025 0.0 6 2007 11 1 2 2 1 1 168 2237 0.0 6 2212 20 0 0 2 2 1 169 2005 0.0 6 1990 14 0 0 0 0 1 170 1921 0.0 6 1910 10 0 0 0 0 1 171 2123 0.0 6 2096 21 1 2 3 172 2211 0.0 6 2191 16 2 0 0 1 1 173 2193 0.0 6 2170 17 2 2 0 1 1 174 1860 0.0 6 1836 20 1 0 1 0 2 175 1889 0.0 6 1875 11 0 0 3 176 2022 0.0 6 2006 11 1 0 2 1 1 177 1555 0.0 6 1549 6 178 1725 0.0 6 1718 7 179 1188 0.0 6 1180 6 0 1 0 1 180 1142 0.0 6 1132 8 0 1 1 181 1169 0.0 6 1164 4 0 0 0 1 182 1099 0.0 6 1089 5 2 0 0 3 183 1518 0.0 6 1507 11 184 1971 0.0 6 1955 11 1 1 2 1 185 1670 0.0 6 1660 8 1 0 0 0 1 186 1137 0.0 6 1129 6 1 0 1 187 1072 0.0 6 1067 4 1 188 979 0.0 6 976 1 1 1 189 821 0.0 6 818 2 0 0 0 1 190 815 0.0 6 806 8 0 0 1 191 880 0.0 6 878 2 192 629 0.0 6 626 3 193 615 0.0 6 609 3 0 2 1 194 565 0.0 6 562 0 2 0 1 195 457 0.0 6 456 0 0 1 196 632 0.0 6 624 6 0 0 2 197 449 0.0 6 445 2 0 1 0 1 198 452 0.0 6 447 5 199 379 0.0 6 375 3 0 1 200 434 0.0 6 433 1 201 255 0.0 6 254 1 202 311 0.0 6 309 2 203 275 0.0 6 273 1 0 0 0 0 1 204 208 0.0 6 208 205 214 0.0 6 209 2 1 1 1 206 200 0.0 6 198 2 207 187 0.0 6 184 3 208 144 0.0 6 144 209 158 0.0 6 156 2 210 120 0.0 6 120 211 139 0.0 6 136 3 212 157 0.0 6 156 1 213 131 0.0 6 131 214 182 0.0 6 181 1 215 110 0.0 6 110 216 103 0.0 6 99 4 217 91 0.0 6 91 218 52 0.0 6 52 219 61 0.0 6 60 1 220 47 0.0 6 46 1 221 54 0.0 6 54 222 65 0.0 6 65 223 40 0.0 6 40 224 29 0.0 6 27 1 1 225 23 0.0 6 22 1 226 29 0.0 6 29 227 21 0.0 6 20 1 228 11 0.0 6 11 229 19 0.0 6 19 230 15 0.0 6 15 231 8 0.0 6 7 1 232 9 0.0 6 9 233 5 0.0 6 5 234 6 0.0 6 6 235 2 0.0 6 2 236 3 0.0 6 3 237 4 0.0 6 4 238 3 0.0 6 3 239 6 0.0 6 6 240 2 0.0 6 2 241 2 0.0 6 2 242 4 0.0 6 3 1 243 1 0.0 6 1 245 2 0.0 6 2 247 1 0.0 6 1 248 1 0.0 6 1 276 1 0.0 6 1 277 4 0.0 6 4 278 18 0.0 6 18 279 92 0.0 6 88 4 280 3 0.0 6 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 3449 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 52 2 0.0 6 2 59 3 0.0 6 3 61 1 0.0 6 1 64 1 0.0 6 1 67 1 0.0 6 1 69 10 0.0 6 10 70 3 0.0 6 3 71 3 0.0 6 3 72 19 0.0 6 19 73 26 0.0 6 26 74 27 0.0 6 27 75 33 0.0 6 33 76 31 0.0 6 30 0 0 1 77 49 0.0 6 49 78 35 0.0 6 35 79 63 0.0 6 63 80 43 0.0 6 42 0 0 0 1 81 32 0.0 6 32 82 28 0.0 6 26 0 0 0 1 1 83 24 0.0 6 24 84 20 0.0 6 19 0 0 0 1 85 25 0.0 6 25 86 14 0.0 6 14 87 9 0.0 6 9 88 22 0.0 6 22 89 26 0.0 6 25 1 90 14 0.0 6 14 91 24 0.0 6 24 92 24 0.0 6 24 93 34 0.0 6 34 94 15 0.0 6 15 95 27 0.0 6 27 96 26 0.0 6 26 97 18 0.0 6 18 98 30 0.0 6 30 99 22 0.0 6 21 0 0 0 1 100 28 0.0 6 28 101 22 0.0 6 22 102 16 0.0 6 16 103 24 0.0 6 24 104 33 0.0 6 33 105 30 0.0 6 30 106 19 0.0 6 19 107 26 0.0 6 26 108 25 0.0 6 25 109 46 0.0 6 46 110 68 0.0 6 68 111 35 0.0 6 34 0 0 1 112 22 0.0 6 21 0 0 0 1 113 14 0.0 6 14 114 35 0.0 6 35 115 15 0.0 6 14 1 116 20 0.0 6 20 117 36 0.0 6 36 118 16 0.0 6 16 119 19 0.0 6 19 120 14 0.0 6 14 121 17 0.0 6 17 122 28 0.0 6 27 0 0 1 123 35 0.0 6 35 124 12 0.0 6 12 125 31 0.0 6 30 0 0 1 126 28 0.0 6 27 0 0 0 1 127 37 0.0 6 37 128 37 0.0 6 37 129 20 0.0 6 20 130 21 0.0 6 21 131 22 0.0 6 22 132 18 0.0 6 18 133 34 0.0 6 34 134 80 0.0 6 80 135 34 0.0 6 34 136 26 0.0 6 26 137 26 0.0 6 26 138 34 0.0 6 34 139 43 0.0 6 43 140 23 0.0 6 23 141 26 0.0 6 26 142 32 0.0 6 32 143 38 0.0 6 38 144 28 0.0 6 28 145 15 0.0 6 15 146 24 0.0 6 24 147 47 0.0 6 47 148 34 0.0 6 34 149 41 0.0 6 39 2 150 42 0.0 6 42 151 27 0.0 6 27 152 33 0.0 6 33 153 15 0.0 6 15 154 29 0.0 6 28 1 155 27 0.0 6 27 156 25 0.0 6 24 0 0 0 1 157 45 0.0 6 44 1 158 41 0.0 6 41 159 23 0.0 6 23 160 21 0.0 6 21 161 19 0.0 6 19 162 35 0.0 6 35 163 48 0.0 6 48 164 41 0.0 6 41 165 35 0.0 6 35 166 28 0.0 6 28 167 36 0.0 6 35 0 0 1 168 23 0.0 6 22 1 169 33 0.0 6 33 170 22 0.0 6 22 171 18 0.0 6 18 172 26 0.0 6 26 173 13 0.0 6 13 174 24 0.0 6 24 175 17 0.0 6 17 176 20 0.0 6 20 177 21 0.0 6 21 178 21 0.0 6 21 179 32 0.0 6 32 180 22 0.0 6 22 181 22 0.0 6 22 182 19 0.0 6 19 183 19 0.0 6 19 184 24 0.0 6 24 185 25 0.0 6 25 186 22 0.0 6 22 187 14 0.0 6 14 188 22 0.0 6 22 189 14 0.0 6 14 190 11 0.0 6 11 191 13 0.0 6 13 192 6 0.0 6 6 193 14 0.0 6 11 1 1 0 0 0 1 194 7 0.0 6 7 195 6 0.0 6 6 196 7 0.0 6 7 197 7 0.0 6 7 198 7 0.0 6 7 199 5 0.0 6 5 200 3 0.0 6 2 1 201 11 0.0 6 11 202 5 0.0 6 5 203 6 0.0 6 6 204 3 0.0 6 3 205 2 0.0 6 2 206 4 0.0 6 4 207 1 0.0 6 1 208 6 0.0 6 6 209 5 0.0 6 5 210 2 0.0 6 2 211 1 0.0 6 1 212 4 0.0 6 4 213 4 0.0 6 4 214 3 0.0 6 3 215 1 0.0 6 1 217 3 0.0 6 3 218 3 0.0 6 3 219 3 0.0 6 3 221 2 0.0 6 2 222 1 0.0 6 1 224 1 0.0 6 1 225 2 0.0 6 2 226 2 0.0 6 2 228 2 0.0 6 2 229 1 0.0 6 1 230 1 0.0 6 1 231 1 0.0 6 1 239 1 0.0 6 1 253 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_08_179_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_08_406_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_08_179_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_08_406_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.55 s (55 µs/read; 1.09 M reads/minute). === Summary === Total read pairs processed: 100,776 Read 1 with adapter: 100,764 (100.0%) Read 2 with adapter: 100,776 (100.0%) == Read fate breakdown == Pairs that were too short: 35 (0.0%) Pairs discarded as untrimmed: 12 (0.0%) Pairs written (passing filters): 100,729 (100.0%) Total basepairs processed: 40,031,627 bp Read 1: 21,601,264 bp Read 2: 18,430,363 bp Total written (filtered): 6,837,003 bp (17.1%) Read 1: 3,533,917 bp Read 2: 3,303,086 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 95809 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 1574.6 0 2 2 4 6 393.7 1 5 1 5 1 98.4 1 1 6 2 24.6 1 0 0 2 7 2 6.2 2 1 0 1 8 1 1.5 2 1 9 3 0.4 2 3 10 1 0.1 3 1 11 1 0.0 3 1 12 1 0.0 3 1 13 1 0.0 3 1 14 2 0.0 4 2 15 8 0.0 4 2 3 2 1 16 8 0.0 4 3 3 2 17 6 0.0 5 4 1 1 19 6 0.0 5 4 2 20 156 0.0 6 151 5 21 71 0.0 6 65 4 2 22 70 0.0 6 67 3 23 48 0.0 6 46 1 1 24 40 0.0 6 38 2 25 37 0.0 6 34 2 1 26 22 0.0 6 21 1 27 17 0.0 6 16 0 0 0 0 0 1 28 17 0.0 6 16 0 0 1 29 8 0.0 6 7 1 30 17 0.0 6 15 2 31 14 0.0 6 14 32 13 0.0 6 13 33 8 0.0 6 8 34 11 0.0 6 10 1 35 10 0.0 6 10 36 7 0.0 6 7 37 7 0.0 6 7 38 6 0.0 6 4 2 39 8 0.0 6 7 0 0 0 0 0 1 40 10 0.0 6 10 41 8 0.0 6 8 42 10 0.0 6 8 2 43 9 0.0 6 9 44 7 0.0 6 6 1 45 6 0.0 6 6 46 7 0.0 6 6 1 47 4 0.0 6 4 48 9 0.0 6 9 49 8 0.0 6 8 50 11 0.0 6 9 1 1 51 6 0.0 6 6 52 13 0.0 6 12 1 53 11 0.0 6 11 54 8 0.0 6 8 55 7 0.0 6 6 0 0 0 0 0 1 56 7 0.0 6 7 57 8 0.0 6 8 58 4 0.0 6 3 0 0 0 0 0 1 59 7 0.0 6 6 0 0 1 60 2 0.0 6 2 61 9 0.0 6 9 62 6 0.0 6 6 63 9 0.0 6 8 1 64 14 0.0 6 14 65 5 0.0 6 5 66 11 0.0 6 11 67 11 0.0 6 11 68 10 0.0 6 10 69 12 0.0 6 12 70 16 0.0 6 15 1 71 20 0.0 6 20 72 15 0.0 6 15 73 15 0.0 6 13 1 0 0 0 1 74 16 0.0 6 16 75 8 0.0 6 7 1 76 13 0.0 6 13 77 9 0.0 6 9 78 21 0.0 6 19 2 79 15 0.0 6 15 80 13 0.0 6 13 81 21 0.0 6 19 2 82 15 0.0 6 15 83 44 0.0 6 43 1 84 19 0.0 6 19 85 19 0.0 6 19 86 19 0.0 6 18 1 87 21 0.0 6 21 88 11 0.0 6 10 1 89 18 0.0 6 17 1 90 13 0.0 6 11 0 1 0 1 91 14 0.0 6 14 92 12 0.0 6 11 1 93 11 0.0 6 11 94 10 0.0 6 10 95 21 0.0 6 21 96 10 0.0 6 10 97 17 0.0 6 17 98 16 0.0 6 16 99 14 0.0 6 14 100 17 0.0 6 17 101 24 0.0 6 21 3 102 31 0.0 6 31 103 28 0.0 6 28 104 30 0.0 6 29 0 1 105 41 0.0 6 41 106 28 0.0 6 27 1 107 36 0.0 6 36 108 22 0.0 6 22 109 34 0.0 6 34 110 38 0.0 6 37 0 1 111 46 0.0 6 46 112 46 0.0 6 45 1 113 65 0.0 6 65 114 53 0.0 6 53 115 73 0.0 6 73 116 65 0.0 6 65 117 61 0.0 6 60 0 0 0 0 1 118 69 0.0 6 68 1 119 70 0.0 6 56 0 1 0 0 12 1 120 54 0.0 6 52 0 0 0 0 2 121 53 0.0 6 52 1 122 42 0.0 6 42 123 58 0.0 6 58 124 52 0.0 6 51 1 125 71 0.0 6 70 1 126 75 0.0 6 75 127 77 0.0 6 77 128 78 0.0 6 77 0 0 0 0 1 129 69 0.0 6 68 1 130 85 0.0 6 84 1 131 75 0.0 6 75 132 84 0.0 6 81 1 0 1 0 1 133 108 0.0 6 108 134 94 0.0 6 94 135 79 0.0 6 78 1 136 120 0.0 6 119 1 137 112 0.0 6 111 1 138 106 0.0 6 106 139 121 0.0 6 120 1 140 161 0.0 6 160 1 141 219 0.0 6 218 1 142 589 0.0 6 588 1 143 1105 0.0 6 1102 3 144 1352 0.0 6 1349 3 145 1315 0.0 6 1313 2 146 1584 0.0 6 1580 3 0 0 0 0 1 147 1882 0.0 6 1878 4 148 2509 0.0 6 2508 1 149 2514 0.0 6 2504 10 150 2026 0.0 6 2022 3 0 0 0 0 1 151 2332 0.0 6 2323 8 0 0 1 152 3341 0.0 6 3334 4 0 1 0 1 1 153 1849 0.0 6 1849 154 1525 0.0 6 1524 1 155 1238 0.0 6 1235 2 1 156 1109 0.0 6 1107 2 157 1184 0.0 6 1183 1 158 1187 0.0 6 1184 2 1 159 1384 0.0 6 1381 3 160 1080 0.0 6 1078 2 161 1191 0.0 6 1188 3 162 1100 0.0 6 1100 163 1186 0.0 6 1183 2 1 164 1045 0.0 6 1043 1 0 0 1 165 910 0.0 6 907 2 0 0 0 1 166 848 0.0 6 847 1 167 867 0.0 6 862 5 168 595 0.0 6 593 2 169 624 0.0 6 621 3 170 712 0.0 6 711 1 171 741 0.0 6 740 1 172 773 0.0 6 772 1 173 836 0.0 6 834 1 0 1 174 808 0.0 6 805 2 0 0 0 0 1 175 949 0.0 6 947 2 176 582 0.0 6 578 3 1 177 481 0.0 6 481 178 419 0.0 6 415 2 1 0 1 179 508 0.0 6 505 3 180 818 0.0 6 816 1 1 181 567 0.0 6 564 3 182 522 0.0 6 517 4 0 1 183 694 0.0 6 694 184 676 0.0 6 672 3 0 0 0 1 185 686 0.0 6 682 3 0 1 186 571 0.0 6 570 1 187 476 0.0 6 475 0 0 0 1 188 539 0.0 6 535 4 189 561 0.0 6 559 2 190 499 0.0 6 498 1 191 576 0.0 6 574 2 192 575 0.0 6 574 1 193 610 0.0 6 607 2 1 194 676 0.0 6 676 195 811 0.0 6 809 1 0 0 0 1 196 664 0.0 6 659 4 0 1 197 808 0.0 6 805 3 198 789 0.0 6 786 3 199 620 0.0 6 619 1 200 578 0.0 6 577 1 201 600 0.0 6 597 3 202 563 0.0 6 557 5 0 0 0 0 1 203 592 0.0 6 590 1 1 204 812 0.0 6 809 1 0 1 0 1 205 658 0.0 6 655 3 206 786 0.0 6 785 1 207 710 0.0 6 705 4 0 0 0 1 208 668 0.0 6 660 7 0 0 0 0 1 209 605 0.0 6 602 3 210 543 0.0 6 540 3 211 651 0.0 6 649 2 212 723 0.0 6 718 4 1 213 677 0.0 6 672 5 214 764 0.0 6 754 7 1 0 0 1 1 215 820 0.0 6 812 8 216 622 0.0 6 616 6 217 675 0.0 6 672 2 0 0 0 1 218 676 0.0 6 669 5 1 0 0 1 219 624 0.0 6 612 11 0 0 1 220 494 0.0 6 494 221 517 0.0 6 516 1 222 543 0.0 6 541 2 223 488 0.0 6 485 3 224 522 0.0 6 517 4 0 0 0 0 1 225 612 0.0 6 609 2 1 226 614 0.0 6 611 3 227 512 0.0 6 511 0 0 1 228 538 0.0 6 535 3 229 496 0.0 6 495 1 230 514 0.0 6 513 0 0 0 1 231 480 0.0 6 479 1 232 525 0.0 6 524 1 233 464 0.0 6 459 5 234 477 0.0 6 476 0 0 0 0 0 1 235 409 0.0 6 409 236 378 0.0 6 377 1 237 427 0.0 6 426 0 1 238 430 0.0 6 425 3 1 1 239 342 0.0 6 341 1 240 398 0.0 6 397 1 241 368 0.0 6 367 1 242 338 0.0 6 337 1 243 330 0.0 6 329 1 244 272 0.0 6 268 4 245 248 0.0 6 244 3 1 246 276 0.0 6 274 1 1 247 263 0.0 6 261 2 248 248 0.0 6 247 1 249 203 0.0 6 203 250 206 0.0 6 203 2 0 0 1 251 188 0.0 6 186 2 252 210 0.0 6 210 253 204 0.0 6 201 3 254 185 0.0 6 185 255 164 0.0 6 163 1 256 161 0.0 6 161 257 174 0.0 6 173 1 258 163 0.0 6 163 259 184 0.0 6 184 260 171 0.0 6 171 261 117 0.0 6 117 262 128 0.0 6 128 263 138 0.0 6 135 3 264 142 0.0 6 141 1 265 110 0.0 6 108 2 266 119 0.0 6 117 2 267 115 0.0 6 113 1 0 0 0 1 268 110 0.0 6 108 1 0 0 1 269 129 0.0 6 127 2 270 99 0.0 6 99 271 97 0.0 6 96 1 272 92 0.0 6 91 1 273 132 0.0 6 131 1 274 110 0.0 6 110 275 201 0.0 6 198 3 276 264 0.0 6 262 2 277 286 0.0 6 283 2 1 278 917 0.0 6 909 8 279 1147 0.0 6 1142 5 280 9 0.0 6 8 1 281 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 4955 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 48 2 0.0 6 2 55 3 0.0 6 3 58 1 0.0 6 1 59 2 0.0 6 1 0 0 0 1 60 1 0.0 6 1 62 40 0.0 6 22 18 63 2001 0.0 6 1939 62 64 3 0.0 6 2 1 65 93 0.0 6 90 3 66 1 0.0 6 1 68 3 0.0 6 1 0 0 0 0 0 2 69 2 0.0 6 1 0 0 0 0 0 1 70 2 0.0 6 2 71 52 0.0 6 52 72 25 0.0 6 25 73 44 0.0 6 44 74 41 0.0 6 39 2 75 44 0.0 6 44 76 53 0.0 6 53 77 48 0.0 6 48 78 68 0.0 6 67 1 79 88 0.0 6 88 80 77 0.0 6 77 81 96 0.0 6 96 82 133 0.0 6 133 83 53 0.0 6 53 84 53 0.0 6 53 85 37 0.0 6 37 86 34 0.0 6 34 87 67 0.0 6 65 1 1 88 41 0.0 6 41 89 42 0.0 6 42 90 50 0.0 6 50 91 50 0.0 6 50 92 40 0.0 6 40 93 36 0.0 6 36 94 25 0.0 6 25 95 34 0.0 6 34 96 32 0.0 6 32 97 21 0.0 6 20 1 98 17 0.0 6 17 99 15 0.0 6 15 100 23 0.0 6 22 1 101 22 0.0 6 21 0 0 0 0 0 1 102 15 0.0 6 15 103 17 0.0 6 17 104 69 0.0 6 69 105 32 0.0 6 32 106 12 0.0 6 12 107 8 0.0 6 8 108 12 0.0 6 12 109 17 0.0 6 17 110 24 0.0 6 24 111 19 0.0 6 19 112 10 0.0 6 10 113 20 0.0 6 20 114 18 0.0 6 18 115 11 0.0 6 11 116 16 0.0 6 16 117 7 0.0 6 7 118 9 0.0 6 9 119 16 0.0 6 16 120 10 0.0 6 10 121 12 0.0 6 11 1 122 8 0.0 6 8 123 10 0.0 6 10 124 12 0.0 6 12 125 19 0.0 6 19 126 8 0.0 6 8 127 6 0.0 6 6 128 11 0.0 6 11 129 10 0.0 6 10 130 5 0.0 6 5 131 10 0.0 6 10 132 15 0.0 6 15 133 12 0.0 6 12 134 16 0.0 6 16 135 10 0.0 6 10 136 13 0.0 6 13 137 7 0.0 6 7 138 9 0.0 6 9 139 8 0.0 6 8 140 6 0.0 6 6 141 9 0.0 6 9 142 10 0.0 6 10 143 6 0.0 6 5 1 144 10 0.0 6 10 145 13 0.0 6 13 146 10 0.0 6 9 1 147 13 0.0 6 13 148 12 0.0 6 12 149 13 0.0 6 13 150 11 0.0 6 11 151 12 0.0 6 12 152 20 0.0 6 20 153 5 0.0 6 5 154 6 0.0 6 6 155 10 0.0 6 10 156 4 0.0 6 4 157 6 0.0 6 6 158 13 0.0 6 13 159 10 0.0 6 10 160 12 0.0 6 12 161 11 0.0 6 11 162 4 0.0 6 4 163 16 0.0 6 16 164 13 0.0 6 13 165 17 0.0 6 17 166 9 0.0 6 9 167 9 0.0 6 9 168 8 0.0 6 8 169 4 0.0 6 4 170 6 0.0 6 6 171 11 0.0 6 11 172 6 0.0 6 6 173 14 0.0 6 14 174 2 0.0 6 2 175 4 0.0 6 4 176 15 0.0 6 15 177 5 0.0 6 5 178 9 0.0 6 9 179 6 0.0 6 6 180 5 0.0 6 5 181 8 0.0 6 8 182 9 0.0 6 9 183 6 0.0 6 6 184 8 0.0 6 8 185 6 0.0 6 6 186 10 0.0 6 10 187 14 0.0 6 14 188 4 0.0 6 4 189 10 0.0 6 10 190 5 0.0 6 5 191 9 0.0 6 9 192 6 0.0 6 6 193 7 0.0 6 7 194 8 0.0 6 8 195 11 0.0 6 11 196 3 0.0 6 3 197 4 0.0 6 4 198 5 0.0 6 5 199 4 0.0 6 4 200 9 0.0 6 9 201 10 0.0 6 10 202 10 0.0 6 10 203 16 0.0 6 16 204 3 0.0 6 3 205 5 0.0 6 5 206 6 0.0 6 6 207 4 0.0 6 4 208 2 0.0 6 2 209 7 0.0 6 7 210 5 0.0 6 5 211 7 0.0 6 7 212 3 0.0 6 3 213 7 0.0 6 6 0 0 1 214 5 0.0 6 5 215 7 0.0 6 7 216 4 0.0 6 3 1 217 5 0.0 6 5 218 5 0.0 6 5 219 6 0.0 6 6 220 9 0.0 6 8 1 221 5 0.0 6 5 222 5 0.0 6 5 223 3 0.0 6 3 224 2 0.0 6 2 225 3 0.0 6 3 226 1 0.0 6 1 227 1 0.0 6 1 228 3 0.0 6 3 229 4 0.0 6 4 230 2 0.0 6 2 231 3 0.0 6 3 232 1 0.0 6 1 233 5 0.0 6 5 234 3 0.0 6 3 235 7 0.0 6 7 236 8 0.0 6 8 237 4 0.0 6 4 238 1 0.0 6 1 240 3 0.0 6 3 241 1 0.0 6 1 242 5 0.0 6 5 243 1 0.0 6 1 244 4 0.0 6 4 245 3 0.0 6 3 246 2 0.0 6 2 247 5 0.0 6 5 249 5 0.0 6 5 250 2 0.0 6 2 251 1 0.0 6 1 252 1 0.0 6 1 253 3 0.0 6 3 254 4 0.0 6 4 255 1 0.0 6 1 256 3 0.0 6 2 0 0 0 0 0 1 258 3 0.0 6 3 259 1 0.0 6 1 260 1 0.0 6 1 261 3 0.0 6 3 264 2 0.0 6 2 265 2 0.0 6 2 266 1 0.0 6 1 268 1 0.0 6 1 269 2 0.0 6 2 271 1 0.0 6 1 272 5 0.0 6 5 273 3 0.0 6 3 275 1 0.0 6 1 281 1 0.0 6 1 282 1 0.0 6 1 283 1 0.0 6 1 284 1 0.0 6 1 285 1 0.0 6 1 286 2 0.0 6 2 287 1 0.0 6 1 296 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 96860 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 6.2 2 1 10 4 0.1 3 4 11 9 0.0 3 7 1 1 12 11 0.0 3 7 4 13 19 0.0 3 18 1 14 20 0.0 4 20 15 7 0.0 4 6 1 16 11 0.0 4 11 17 48 0.0 5 41 6 1 18 25 0.0 5 21 3 0 1 19 29 0.0 5 26 3 20 95 0.0 6 91 4 21 47 0.0 6 47 22 43 0.0 6 39 4 23 27 0.0 6 24 3 24 25 0.0 6 23 2 25 34 0.0 6 32 2 26 26 0.0 6 25 1 27 20 0.0 6 19 1 28 16 0.0 6 15 1 29 20 0.0 6 18 1 1 30 24 0.0 6 21 2 1 31 24 0.0 6 21 3 32 13 0.0 6 11 1 1 33 13 0.0 6 11 2 34 21 0.0 6 18 3 35 17 0.0 6 15 2 36 31 0.0 6 30 1 37 19 0.0 6 15 2 1 0 0 1 38 15 0.0 6 14 1 39 7 0.0 6 6 1 40 17 0.0 6 16 0 0 1 41 18 0.0 6 15 3 42 16 0.0 6 14 1 1 43 20 0.0 6 19 1 44 19 0.0 6 18 0 0 0 0 1 45 24 0.0 6 23 1 46 16 0.0 6 14 2 47 28 0.0 6 26 2 48 23 0.0 6 23 49 20 0.0 6 19 1 50 40 0.0 6 38 2 51 33 0.0 6 30 3 52 38 0.0 6 36 2 53 32 0.0 6 28 4 54 22 0.0 6 19 2 1 55 33 0.0 6 32 1 56 53 0.0 6 51 2 57 33 0.0 6 31 1 1 58 33 0.0 6 32 1 59 48 0.0 6 46 2 60 35 0.0 6 33 2 61 39 0.0 6 35 3 0 1 62 33 0.0 6 29 4 63 50 0.0 6 46 4 64 60 0.0 6 51 8 0 1 65 40 0.0 6 40 66 37 0.0 6 36 1 67 76 0.0 6 72 2 0 1 0 0 1 68 175 0.0 6 169 5 0 1 69 79 0.0 6 77 2 70 103 0.0 6 99 2 1 1 71 70 0.0 6 67 2 0 0 1 72 86 0.0 6 83 3 73 36 0.0 6 34 2 74 58 0.0 6 58 75 66 0.0 6 63 3 76 54 0.0 6 51 2 1 77 65 0.0 6 61 3 0 0 0 0 1 78 57 0.0 6 54 3 79 41 0.0 6 40 1 80 66 0.0 6 65 1 81 83 0.0 6 79 3 1 82 95 0.0 6 92 3 83 82 0.0 6 79 1 0 1 0 0 1 84 95 0.0 6 91 1 1 1 0 1 85 56 0.0 6 54 2 86 66 0.0 6 65 0 1 87 77 0.0 6 70 5 0 1 0 0 1 88 74 0.0 6 73 1 89 64 0.0 6 61 3 90 70 0.0 6 68 0 0 1 0 1 91 103 0.0 6 95 8 92 94 0.0 6 90 4 93 98 0.0 6 98 94 122 0.0 6 120 2 95 100 0.0 6 98 2 96 116 0.0 6 113 2 0 0 0 0 1 97 134 0.0 6 130 3 0 0 0 0 1 98 104 0.0 6 104 99 133 0.0 6 130 2 0 0 0 1 100 90 0.0 6 86 2 1 0 0 1 101 123 0.0 6 120 3 102 111 0.0 6 108 3 103 116 0.0 6 109 7 104 159 0.0 6 156 1 1 0 0 0 1 105 244 0.0 6 240 4 106 148 0.0 6 145 3 107 165 0.0 6 163 2 108 138 0.0 6 130 7 0 0 0 0 1 109 169 0.0 6 163 4 0 1 1 110 143 0.0 6 140 2 0 1 111 167 0.0 6 162 2 0 1 0 1 1 112 192 0.0 6 190 2 113 262 0.0 6 258 4 114 246 0.0 6 241 4 1 115 206 0.0 6 205 1 116 241 0.0 6 239 1 0 0 1 117 217 0.0 6 214 2 1 118 259 0.0 6 258 1 119 294 0.0 6 292 2 120 278 0.0 6 273 5 121 276 0.0 6 272 3 1 122 290 0.0 6 286 3 1 123 292 0.0 6 289 3 124 346 0.0 6 341 4 0 0 0 0 1 125 356 0.0 6 349 4 1 0 0 0 2 126 432 0.0 6 425 6 1 127 488 0.0 6 480 6 1 1 128 462 0.0 6 453 9 129 602 0.0 6 593 6 1 2 130 705 0.0 6 680 22 1 0 0 1 1 131 1643 0.0 6 1600 40 0 1 0 1 1 132 727 0.0 6 719 7 0 0 0 0 1 133 671 0.0 6 661 7 1 2 134 655 0.0 6 643 10 0 0 1 0 1 135 782 0.0 6 767 14 0 0 1 136 763 0.0 6 752 11 137 650 0.0 6 645 4 1 138 667 0.0 6 658 7 0 0 0 0 2 139 744 0.0 6 732 10 1 0 0 1 140 1116 0.0 6 1109 6 0 0 0 1 141 1502 0.0 6 1492 10 142 1902 0.0 6 1892 9 0 0 0 1 143 2035 0.0 6 2021 10 0 1 2 0 1 144 1876 0.0 6 1871 4 0 0 0 0 1 145 2426 0.0 6 2415 10 0 0 0 1 146 2671 0.0 6 2661 8 0 1 0 0 1 147 2501 0.0 6 2489 12 148 2523 0.0 6 2507 13 1 0 1 1 149 2995 0.0 6 2981 11 0 1 1 1 150 3317 0.0 6 3307 6 2 0 2 151 3137 0.0 6 3118 14 3 0 0 2 152 2162 0.0 6 2146 13 1 0 0 2 153 2123 0.0 6 2106 15 0 0 0 0 2 154 2186 0.0 6 2177 7 1 0 0 1 155 1758 0.0 6 1742 12 1 0 1 2 156 1853 0.0 6 1838 11 1 1 1 0 1 157 1632 0.0 6 1624 6 0 1 1 158 1710 0.0 6 1698 11 0 0 1 159 1701 0.0 6 1689 10 1 0 0 0 1 160 1482 0.0 6 1472 5 2 0 1 1 1 161 1610 0.0 6 1600 9 0 0 0 1 162 1257 0.0 6 1250 5 1 0 1 163 1142 0.0 6 1131 10 0 1 164 1174 0.0 6 1170 4 165 968 0.0 6 962 5 0 0 0 1 166 962 0.0 6 954 8 167 1193 0.0 6 1185 6 0 1 1 168 1005 0.0 6 995 9 0 0 0 0 1 169 1228 0.0 6 1222 4 0 1 1 170 1055 0.0 6 1045 5 1 0 1 3 171 1028 0.0 6 1025 2 0 0 0 0 1 172 1120 0.0 6 1112 4 0 1 1 1 1 173 1130 0.0 6 1118 11 1 174 975 0.0 6 965 10 175 947 0.0 6 940 6 1 176 844 0.0 6 837 6 1 177 963 0.0 6 958 4 0 0 0 1 178 753 0.0 6 747 5 0 0 0 1 179 732 0.0 6 726 5 0 0 0 1 180 592 0.0 6 588 4 181 544 0.0 6 542 2 182 597 0.0 6 593 3 0 0 0 0 1 183 503 0.0 6 498 4 0 0 0 1 184 718 0.0 6 711 5 1 0 0 1 185 923 0.0 6 917 3 2 0 1 186 753 0.0 6 744 5 0 2 0 1 1 187 569 0.0 6 563 6 188 515 0.0 6 510 4 1 189 485 0.0 6 484 1 190 468 0.0 6 464 2 0 2 191 391 0.0 6 390 1 192 399 0.0 6 397 1 0 1 193 334 0.0 6 332 1 1 194 298 0.0 6 296 2 195 267 0.0 6 265 2 196 230 0.0 6 229 1 197 321 0.0 6 319 2 198 228 0.0 6 227 0 0 0 1 199 213 0.0 6 210 3 200 218 0.0 6 214 4 201 184 0.0 6 184 202 169 0.0 6 168 1 203 167 0.0 6 167 204 152 0.0 6 151 1 205 160 0.0 6 159 1 206 143 0.0 6 143 207 125 0.0 6 123 2 208 101 0.0 6 101 209 93 0.0 6 93 210 110 0.0 6 108 1 1 211 83 0.0 6 83 212 79 0.0 6 79 213 84 0.0 6 83 1 214 109 0.0 6 107 2 215 101 0.0 6 98 3 216 79 0.0 6 76 3 217 48 0.0 6 48 218 48 0.0 6 47 1 219 48 0.0 6 48 220 45 0.0 6 45 221 39 0.0 6 39 222 58 0.0 6 57 1 223 39 0.0 6 39 224 41 0.0 6 40 1 225 15 0.0 6 15 226 30 0.0 6 30 227 20 0.0 6 20 228 16 0.0 6 14 2 229 16 0.0 6 16 230 16 0.0 6 16 231 14 0.0 6 13 1 232 17 0.0 6 17 233 19 0.0 6 19 234 17 0.0 6 17 235 25 0.0 6 24 1 236 14 0.0 6 13 1 237 14 0.0 6 14 238 13 0.0 6 13 239 10 0.0 6 9 1 240 6 0.0 6 6 241 12 0.0 6 12 242 9 0.0 6 9 243 7 0.0 6 7 244 2 0.0 6 2 245 3 0.0 6 3 246 3 0.0 6 3 247 2 0.0 6 2 248 4 0.0 6 4 249 5 0.0 6 5 250 3 0.0 6 2 1 251 3 0.0 6 3 252 4 0.0 6 4 255 2 0.0 6 2 278 2 0.0 6 2 279 10 0.0 6 8 2 280 64 0.0 6 63 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 3916 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 6 91 24.6 1 0 0 91 48 16 0.0 6 0 15 1 51 1 0.0 6 1 52 2 0.0 6 2 54 1 0.0 6 1 56 9 0.0 6 9 57 3 0.0 6 3 58 2 0.0 6 2 61 1 0.0 6 1 62 2 0.0 6 2 63 1 0.0 6 1 64 11 0.0 6 11 65 23 0.0 6 23 68 5 0.0 6 5 69 1 0.0 6 0 1 70 29 0.0 6 29 71 31 0.0 6 31 72 66 0.0 6 66 73 50 0.0 6 50 74 73 0.0 6 72 0 0 0 1 75 57 0.0 6 56 0 0 1 76 67 0.0 6 67 77 117 0.0 6 117 78 251 0.0 6 250 1 79 103 0.0 6 103 80 125 0.0 6 124 0 0 0 1 81 157 0.0 6 155 2 82 91 0.0 6 88 0 0 2 1 83 72 0.0 6 71 0 0 1 84 74 0.0 6 72 1 0 0 1 85 45 0.0 6 44 0 0 0 1 86 63 0.0 6 63 87 51 0.0 6 51 88 172 0.0 6 170 1 0 1 89 39 0.0 6 37 0 0 0 2 90 51 0.0 6 51 91 48 0.0 6 46 0 0 1 1 92 53 0.0 6 53 93 46 0.0 6 45 0 0 0 1 94 56 0.0 6 56 95 25 0.0 6 25 96 47 0.0 6 47 97 28 0.0 6 28 98 25 0.0 6 25 99 26 0.0 6 26 100 19 0.0 6 19 101 37 0.0 6 37 102 42 0.0 6 41 0 0 0 1 103 25 0.0 6 25 104 43 0.0 6 43 105 21 0.0 6 21 106 15 0.0 6 14 0 0 0 1 107 21 0.0 6 21 108 16 0.0 6 16 109 34 0.0 6 34 110 16 0.0 6 16 111 16 0.0 6 16 112 47 0.0 6 46 0 0 1 113 21 0.0 6 18 3 114 115 0.0 6 111 4 115 22 0.0 6 22 116 14 0.0 6 14 117 59 0.0 6 58 0 0 1 118 25 0.0 6 25 119 17 0.0 6 17 120 17 0.0 6 17 121 19 0.0 6 19 122 19 0.0 6 18 0 0 0 1 123 15 0.0 6 15 124 21 0.0 6 21 125 11 0.0 6 11 126 11 0.0 6 11 127 10 0.0 6 10 128 24 0.0 6 24 129 12 0.0 6 12 130 13 0.0 6 13 131 13 0.0 6 13 132 18 0.0 6 18 133 29 0.0 6 29 134 8 0.0 6 8 135 14 0.0 6 14 136 17 0.0 6 17 137 15 0.0 6 14 0 0 0 1 138 25 0.0 6 25 139 8 0.0 6 8 140 13 0.0 6 13 141 21 0.0 6 20 1 142 34 0.0 6 34 143 12 0.0 6 12 144 7 0.0 6 7 145 17 0.0 6 17 146 9 0.0 6 9 147 11 0.0 6 11 148 14 0.0 6 14 149 14 0.0 6 14 150 8 0.0 6 8 151 13 0.0 6 13 152 2 0.0 6 2 153 46 0.0 6 46 154 10 0.0 6 10 155 6 0.0 6 6 156 14 0.0 6 14 157 15 0.0 6 15 158 11 0.0 6 11 159 6 0.0 6 6 160 4 0.0 6 4 161 8 0.0 6 8 162 18 0.0 6 17 0 0 0 0 1 163 18 0.0 6 18 164 9 0.0 6 9 165 6 0.0 6 6 166 12 0.0 6 12 167 23 0.0 6 22 1 168 7 0.0 6 7 169 11 0.0 6 11 170 7 0.0 6 7 171 7 0.0 6 7 172 7 0.0 6 7 173 15 0.0 6 15 174 8 0.0 6 8 175 7 0.0 6 7 176 16 0.0 6 16 177 7 0.0 6 7 178 7 0.0 6 7 179 6 0.0 6 6 180 4 0.0 6 4 181 4 0.0 6 4 182 19 0.0 6 19 183 9 0.0 6 9 184 4 0.0 6 4 185 12 0.0 6 11 0 0 1 186 4 0.0 6 4 187 6 0.0 6 6 188 5 0.0 6 5 189 8 0.0 6 8 190 6 0.0 6 6 191 7 0.0 6 7 192 5 0.0 6 5 193 4 0.0 6 4 194 1 0.0 6 1 195 3 0.0 6 3 196 2 0.0 6 2 197 6 0.0 6 6 198 4 0.0 6 3 0 0 0 1 199 5 0.0 6 5 200 7 0.0 6 7 201 1 0.0 6 1 202 6 0.0 6 6 203 6 0.0 6 6 204 4 0.0 6 4 205 4 0.0 6 4 206 2 0.0 6 1 0 0 0 0 1 208 2 0.0 6 2 209 1 0.0 6 1 210 2 0.0 6 2 211 4 0.0 6 4 212 1 0.0 6 1 213 1 0.0 6 1 214 3 0.0 6 3 216 1 0.0 6 1 218 2 0.0 6 2 219 2 0.0 6 2 223 2 0.0 6 2 224 3 0.0 6 3 230 2 0.0 6 2 231 1 0.0 6 1 233 1 0.0 6 1 242 1 0.0 6 1 243 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_11_168_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_11_395_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_11_168_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_11_395_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_12_169_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_12_396_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_12_169_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_12_396_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_13_170_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_16-Rev_13_397_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_13_170_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_16-Rev_13_397_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_07_171_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_07_398_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_07_171_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_07_398_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_08_172_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_08_399_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_08_172_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_08_399_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_09_173_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_09_400_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_09_173_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_09_400_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_10_174_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_10_401_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_10_174_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_10_401_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_11_175_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_11_402_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_11_175_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_11_402_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_12_176_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_12_403_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_12_176_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_12_403_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_13_177_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_17-Rev_13_404_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_13_177_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_17-Rev_13_404_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_07_178_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_07_405_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_07_178_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_07_405_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_08_179_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_08_406_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_08_179_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_08_406_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_09_180_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_09_407_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_09_180_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_09_407_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.05 s (59 µs/read; 1.02 M reads/minute). === Summary === Total read pairs processed: 85,811 Read 1 with adapter: 85,804 (100.0%) Read 2 with adapter: 85,754 (99.9%) == Read fate breakdown == Pairs that were too short: 57 (0.1%) Pairs discarded as untrimmed: 63 (0.1%) Pairs written (passing filters): 85,691 (99.9%) Total basepairs processed: 36,667,433 bp Read 1: 20,313,293 bp Read 2: 16,354,140 bp Total written (filtered): 5,433,377 bp (14.8%) Read 1: 2,866,407 bp Read 2: 2,566,970 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 83258 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1340.8 0 1 4 2 335.2 1 2 5 1 83.8 1 1 6 1 20.9 1 0 0 1 7 3 5.2 2 2 0 1 9 1 0.3 2 1 14 2 0.0 4 2 15 4 0.0 4 4 17 5 0.0 5 4 1 19 4 0.0 5 3 1 20 89 0.0 6 86 3 21 68 0.0 6 63 5 22 44 0.0 6 41 3 23 23 0.0 6 23 24 22 0.0 6 19 2 1 25 24 0.0 6 24 26 23 0.0 6 23 27 18 0.0 6 16 2 28 18 0.0 6 17 1 29 8 0.0 6 6 2 30 14 0.0 6 14 31 6 0.0 6 6 32 7 0.0 6 7 33 5 0.0 6 4 1 34 7 0.0 6 7 35 9 0.0 6 9 36 11 0.0 6 11 37 4 0.0 6 4 38 3 0.0 6 3 39 3 0.0 6 3 40 13 0.0 6 12 0 0 0 1 41 14 0.0 6 13 1 42 7 0.0 6 7 43 7 0.0 6 6 1 44 16 0.0 6 16 45 10 0.0 6 8 2 46 14 0.0 6 13 0 0 1 47 7 0.0 6 7 48 6 0.0 6 6 49 11 0.0 6 11 50 4 0.0 6 3 1 51 6 0.0 6 6 52 4 0.0 6 3 1 53 8 0.0 6 7 1 54 4 0.0 6 4 55 3 0.0 6 3 56 4 0.0 6 4 57 2 0.0 6 2 58 4 0.0 6 3 0 0 0 1 59 3 0.0 6 3 60 2 0.0 6 2 61 5 0.0 6 5 62 4 0.0 6 4 63 5 0.0 6 4 1 64 11 0.0 6 10 1 65 7 0.0 6 4 1 1 0 1 66 8 0.0 6 7 1 67 5 0.0 6 5 68 5 0.0 6 5 69 7 0.0 6 7 70 7 0.0 6 6 1 71 8 0.0 6 8 72 11 0.0 6 11 73 13 0.0 6 13 74 4 0.0 6 4 75 12 0.0 6 12 76 10 0.0 6 10 77 8 0.0 6 7 1 78 7 0.0 6 7 79 6 0.0 6 5 1 80 15 0.0 6 15 81 11 0.0 6 11 82 8 0.0 6 7 1 83 9 0.0 6 9 84 15 0.0 6 15 85 7 0.0 6 7 86 10 0.0 6 9 1 87 16 0.0 6 15 1 88 9 0.0 6 9 89 13 0.0 6 13 90 1 0.0 6 1 91 7 0.0 6 6 1 92 12 0.0 6 12 93 5 0.0 6 5 94 5 0.0 6 4 1 95 10 0.0 6 10 96 9 0.0 6 9 97 10 0.0 6 10 98 7 0.0 6 6 1 99 12 0.0 6 12 100 15 0.0 6 14 1 101 14 0.0 6 14 102 13 0.0 6 12 1 103 17 0.0 6 16 1 104 24 0.0 6 24 105 16 0.0 6 16 106 21 0.0 6 20 1 107 13 0.0 6 13 108 20 0.0 6 19 1 109 19 0.0 6 17 2 110 20 0.0 6 19 1 111 26 0.0 6 26 112 26 0.0 6 26 113 27 0.0 6 25 2 114 25 0.0 6 24 1 115 33 0.0 6 31 1 0 0 0 1 116 18 0.0 6 18 117 18 0.0 6 18 118 27 0.0 6 27 119 22 0.0 6 22 120 29 0.0 6 29 121 22 0.0 6 22 122 26 0.0 6 26 123 30 0.0 6 30 124 29 0.0 6 29 125 32 0.0 6 31 0 0 1 126 34 0.0 6 34 127 34 0.0 6 33 1 128 36 0.0 6 36 129 36 0.0 6 33 2 0 0 0 1 130 48 0.0 6 47 1 131 37 0.0 6 36 1 132 41 0.0 6 41 133 36 0.0 6 36 134 45 0.0 6 44 1 135 34 0.0 6 34 136 40 0.0 6 39 1 137 58 0.0 6 58 138 68 0.0 6 67 0 1 139 57 0.0 6 55 2 140 195 0.0 6 195 141 209 0.0 6 208 1 142 475 0.0 6 475 143 223 0.0 6 221 2 144 268 0.0 6 266 2 145 360 0.0 6 358 1 0 0 0 0 1 146 619 0.0 6 618 1 147 430 0.0 6 427 3 148 672 0.0 6 672 149 388 0.0 6 384 4 150 387 0.0 6 386 1 151 299 0.0 6 298 1 152 424 0.0 6 421 3 153 442 0.0 6 441 1 154 241 0.0 6 239 2 155 290 0.0 6 290 156 304 0.0 6 304 157 710 0.0 6 710 158 606 0.0 6 602 3 1 159 673 0.0 6 672 1 160 516 0.0 6 514 2 161 502 0.0 6 500 2 162 550 0.0 6 550 163 594 0.0 6 592 2 164 431 0.0 6 430 1 165 575 0.0 6 569 4 1 0 0 1 166 572 0.0 6 569 2 0 0 0 1 167 757 0.0 6 754 3 168 596 0.0 6 595 1 169 284 0.0 6 280 4 170 614 0.0 6 614 171 600 0.0 6 598 2 172 624 0.0 6 621 3 173 564 0.0 6 560 4 174 877 0.0 6 874 3 175 809 0.0 6 808 0 1 176 630 0.0 6 626 3 0 0 0 1 177 266 0.0 6 263 3 178 776 0.0 6 772 4 179 470 0.0 6 468 2 180 718 0.0 6 716 2 181 279 0.0 6 278 1 182 288 0.0 6 285 3 183 597 0.0 6 597 184 478 0.0 6 475 3 185 316 0.0 6 313 2 0 0 0 1 186 630 0.0 6 628 2 187 622 0.0 6 619 3 188 796 0.0 6 794 1 1 189 1002 0.0 6 997 5 190 468 0.0 6 467 0 1 191 400 0.0 6 398 1 0 0 0 1 192 452 0.0 6 446 6 193 626 0.0 6 623 2 1 194 631 0.0 6 626 5 195 838 0.0 6 835 3 196 1351 0.0 6 1346 5 197 1451 0.0 6 1442 9 198 816 0.0 6 801 14 1 199 681 0.0 6 678 2 0 0 0 1 200 563 0.0 6 559 3 0 0 0 0 1 201 676 0.0 6 670 6 202 666 0.0 6 659 5 0 1 0 1 203 823 0.0 6 819 4 204 933 0.0 6 926 6 1 205 1021 0.0 6 1012 8 0 0 0 0 1 206 830 0.0 6 821 9 207 877 0.0 6 867 8 0 0 0 2 208 940 0.0 6 932 7 0 0 0 0 1 209 936 0.0 6 929 6 0 1 210 703 0.0 6 699 4 211 945 0.0 6 941 4 212 1033 0.0 6 1026 6 0 0 1 213 1176 0.0 6 1172 2 1 0 0 0 1 214 1218 0.0 6 1208 10 215 1587 0.0 6 1578 7 1 0 0 1 216 960 0.0 6 950 9 0 0 0 1 217 1390 0.0 6 1384 5 1 218 1030 0.0 6 1027 2 0 1 219 785 0.0 6 781 4 220 716 0.0 6 714 2 221 732 0.0 6 728 4 222 788 0.0 6 784 3 0 1 223 703 0.0 6 700 2 0 0 0 1 224 735 0.0 6 732 2 1 225 964 0.0 6 959 5 226 890 0.0 6 878 10 0 0 0 2 227 684 0.0 6 678 4 0 1 0 1 228 570 0.0 6 569 1 229 607 0.0 6 605 2 230 743 0.0 6 738 5 231 669 0.0 6 666 2 0 0 1 232 838 0.0 6 833 5 233 642 0.0 6 641 1 234 530 0.0 6 528 2 235 564 0.0 6 560 4 236 565 0.0 6 555 6 3 1 237 447 0.0 6 445 2 238 521 0.0 6 520 1 239 527 0.0 6 523 3 0 0 1 240 604 0.0 6 600 4 241 517 0.0 6 516 1 242 605 0.0 6 597 8 243 512 0.0 6 507 5 244 466 0.0 6 463 2 0 0 0 0 1 245 536 0.0 6 531 5 246 537 0.0 6 532 5 247 442 0.0 6 441 0 1 248 427 0.0 6 426 1 249 317 0.0 6 314 3 250 381 0.0 6 376 5 251 412 0.0 6 408 2 1 1 252 273 0.0 6 271 2 253 233 0.0 6 230 3 254 260 0.0 6 257 2 0 0 0 0 1 255 220 0.0 6 216 3 0 0 0 1 256 251 0.0 6 250 0 1 257 273 0.0 6 271 2 258 213 0.0 6 210 3 259 254 0.0 6 252 1 1 260 216 0.0 6 215 1 261 185 0.0 6 183 1 0 0 0 0 1 262 212 0.0 6 208 3 0 1 263 239 0.0 6 236 3 264 186 0.0 6 183 3 265 208 0.0 6 203 5 266 173 0.0 6 173 267 206 0.0 6 206 268 207 0.0 6 204 1 2 269 215 0.0 6 210 5 270 139 0.0 6 138 1 271 143 0.0 6 142 1 272 159 0.0 6 158 1 273 205 0.0 6 202 2 0 0 1 274 301 0.0 6 296 5 275 243 0.0 6 240 2 1 276 335 0.0 6 332 3 277 561 0.0 6 549 12 278 978 0.0 6 960 15 2 0 0 1 279 2512 0.0 6 2469 42 1 280 13 0.0 6 12 1 281 1 0.0 6 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2546 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 99 0.0 6 97 2 71 2 0.0 6 2 72 6 0.0 6 6 73 11 0.0 6 11 74 13 0.0 6 13 75 13 0.0 6 13 76 6 0.0 6 6 77 13 0.0 6 13 78 19 0.0 6 19 79 14 0.0 6 14 80 15 0.0 6 15 81 9 0.0 6 9 82 12 0.0 6 12 83 8 0.0 6 8 84 12 0.0 6 12 85 15 0.0 6 15 86 6 0.0 6 6 87 7 0.0 6 7 88 15 0.0 6 15 89 34 0.0 6 34 90 11 0.0 6 10 0 0 0 0 0 1 91 18 0.0 6 18 92 15 0.0 6 15 93 18 0.0 6 18 94 13 0.0 6 12 1 95 16 0.0 6 16 96 11 0.0 6 11 97 28 0.0 6 28 98 25 0.0 6 25 99 26 0.0 6 25 1 100 18 0.0 6 18 101 5 0.0 6 5 102 20 0.0 6 20 103 16 0.0 6 16 104 20 0.0 6 20 105 24 0.0 6 24 106 27 0.0 6 27 107 21 0.0 6 21 108 24 0.0 6 24 109 7 0.0 6 7 110 24 0.0 6 24 111 20 0.0 6 20 112 23 0.0 6 23 113 4 0.0 6 4 114 11 0.0 6 11 115 18 0.0 6 18 116 7 0.0 6 7 117 11 0.0 6 11 118 25 0.0 6 25 119 14 0.0 6 14 120 30 0.0 6 30 121 31 0.0 6 31 122 5 0.0 6 5 123 5 0.0 6 5 124 8 0.0 6 8 125 15 0.0 6 15 126 9 0.0 6 9 127 20 0.0 6 20 128 29 0.0 6 29 129 41 0.0 6 41 130 4 0.0 6 4 131 5 0.0 6 5 132 7 0.0 6 7 133 11 0.0 6 11 134 13 0.0 6 13 135 18 0.0 6 18 136 24 0.0 6 23 1 137 19 0.0 6 19 138 11 0.0 6 10 1 139 11 0.0 6 11 140 17 0.0 6 17 141 20 0.0 6 20 142 13 0.0 6 13 143 16 0.0 6 16 144 9 0.0 6 9 145 15 0.0 6 15 146 14 0.0 6 14 147 17 0.0 6 17 148 29 0.0 6 29 149 49 0.0 6 49 150 9 0.0 6 9 151 14 0.0 6 14 152 8 0.0 6 8 153 11 0.0 6 11 154 30 0.0 6 30 155 12 0.0 6 12 156 6 0.0 6 6 157 16 0.0 6 16 158 17 0.0 6 17 159 7 0.0 6 7 160 3 0.0 6 3 161 4 0.0 6 4 162 8 0.0 6 8 163 17 0.0 6 17 164 28 0.0 6 28 165 11 0.0 6 11 166 17 0.0 6 17 167 18 0.0 6 18 168 10 0.0 6 10 169 9 0.0 6 9 170 7 0.0 6 7 171 17 0.0 6 17 172 24 0.0 6 24 173 5 0.0 6 5 174 11 0.0 6 11 175 16 0.0 6 16 176 17 0.0 6 17 177 29 0.0 6 29 178 43 0.0 6 43 179 20 0.0 6 20 180 18 0.0 6 18 181 8 0.0 6 8 182 22 0.0 6 22 183 33 0.0 6 33 184 11 0.0 6 10 1 185 12 0.0 6 12 186 24 0.0 6 24 187 6 0.0 6 6 188 23 0.0 6 23 189 8 0.0 6 8 190 22 0.0 6 22 191 15 0.0 6 15 192 7 0.0 6 7 193 11 0.0 6 11 194 11 0.0 6 11 195 13 0.0 6 13 196 21 0.0 6 21 197 8 0.0 6 8 198 11 0.0 6 11 199 3 0.0 6 3 200 12 0.0 6 12 201 7 0.0 6 7 202 8 0.0 6 8 203 3 0.0 6 3 204 8 0.0 6 8 205 9 0.0 6 9 206 7 0.0 6 7 207 11 0.0 6 11 208 2 0.0 6 2 209 6 0.0 6 6 210 5 0.0 6 5 211 8 0.0 6 8 212 18 0.0 6 18 213 4 0.0 6 4 214 8 0.0 6 8 215 4 0.0 6 3 1 216 11 0.0 6 11 217 14 0.0 6 14 218 2 0.0 6 2 219 2 0.0 6 2 220 3 0.0 6 3 221 11 0.0 6 11 222 1 0.0 6 1 224 5 0.0 6 4 1 225 5 0.0 6 5 226 9 0.0 6 9 227 16 0.0 6 16 228 13 0.0 6 13 229 11 0.0 6 11 230 23 0.0 6 23 231 3 0.0 6 3 232 6 0.0 6 6 233 9 0.0 6 9 234 2 0.0 6 2 235 8 0.0 6 8 236 2 0.0 6 2 237 5 0.0 6 5 238 1 0.0 6 1 239 5 0.0 6 5 240 5 0.0 6 5 241 8 0.0 6 8 242 2 0.0 6 2 243 4 0.0 6 4 244 17 0.0 6 17 245 27 0.0 6 27 246 2 0.0 6 2 247 4 0.0 6 4 248 3 0.0 6 3 249 6 0.0 6 6 250 4 0.0 6 4 252 2 0.0 6 2 253 1 0.0 6 1 255 1 0.0 6 0 1 256 9 0.0 6 9 257 7 0.0 6 7 258 5 0.0 6 5 259 2 0.0 6 2 260 3 0.0 6 3 261 6 0.0 6 6 262 1 0.0 6 1 263 2 0.0 6 2 264 2 0.0 6 2 265 1 0.0 6 1 266 2 0.0 6 2 267 5 0.0 6 5 268 3 0.0 6 3 269 1 0.0 6 1 271 1 0.0 6 1 273 3 0.0 6 3 274 2 0.0 6 2 277 2 0.0 6 2 278 1 0.0 6 1 279 2 0.0 6 2 281 1 0.0 6 1 288 1 0.0 6 1 292 1 0.0 6 1 295 1 0.0 6 1 296 1 0.0 6 1 297 2 0.0 6 2 298 6 0.0 6 6 302 1 0.0 6 1 303 1 0.0 6 1 306 1 0.0 6 1 310 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 83402 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 335.2 1 1 5 1 83.8 1 1 7 4 5.2 2 4 10 1 0.1 3 1 11 2 0.0 3 2 12 9 0.0 3 7 1 0 1 13 5 0.0 3 3 2 14 13 0.0 4 13 15 2 0.0 4 1 1 16 5 0.0 4 4 0 1 17 26 0.0 5 25 1 18 7 0.0 5 6 0 1 19 47 0.0 5 46 1 20 124 0.0 6 120 4 21 51 0.0 6 47 4 22 46 0.0 6 44 2 23 28 0.0 6 28 24 19 0.0 6 19 25 17 0.0 6 17 26 21 0.0 6 19 2 27 15 0.0 6 12 2 0 0 1 28 20 0.0 6 16 4 29 16 0.0 6 16 30 13 0.0 6 12 0 0 1 31 13 0.0 6 12 0 0 0 1 32 20 0.0 6 16 4 33 18 0.0 6 17 1 34 12 0.0 6 10 1 0 0 1 35 10 0.0 6 10 36 19 0.0 6 15 2 0 0 1 0 1 37 11 0.0 6 10 1 38 18 0.0 6 15 2 0 0 1 39 12 0.0 6 11 1 40 13 0.0 6 12 0 1 41 13 0.0 6 11 2 42 7 0.0 6 7 43 10 0.0 6 8 0 1 1 44 13 0.0 6 11 0 2 45 13 0.0 6 10 2 0 1 46 21 0.0 6 19 1 0 1 47 20 0.0 6 18 0 1 1 48 28 0.0 6 25 2 1 49 21 0.0 6 20 0 1 50 20 0.0 6 18 2 51 29 0.0 6 26 3 52 23 0.0 6 21 1 0 0 0 1 53 22 0.0 6 20 1 1 54 19 0.0 6 17 1 1 55 26 0.0 6 25 0 0 0 1 56 23 0.0 6 21 2 57 22 0.0 6 19 1 1 0 1 58 27 0.0 6 24 1 1 0 1 59 21 0.0 6 21 60 26 0.0 6 26 61 33 0.0 6 30 3 62 28 0.0 6 26 2 63 21 0.0 6 17 4 64 38 0.0 6 35 2 0 0 0 0 1 65 45 0.0 6 43 2 66 41 0.0 6 37 2 0 1 1 67 39 0.0 6 37 2 68 39 0.0 6 34 4 1 69 46 0.0 6 43 2 1 70 188 0.0 6 177 9 1 0 1 71 75 0.0 6 68 6 0 0 1 72 82 0.0 6 77 5 73 41 0.0 6 37 2 0 0 1 1 74 57 0.0 6 55 1 0 0 0 1 75 39 0.0 6 36 2 0 1 76 50 0.0 6 47 2 0 0 1 77 44 0.0 6 39 1 1 1 1 0 1 78 38 0.0 6 35 2 0 0 0 0 1 79 45 0.0 6 42 1 0 2 80 53 0.0 6 49 3 1 81 55 0.0 6 53 2 82 66 0.0 6 60 6 83 68 0.0 6 64 3 0 0 0 0 1 84 62 0.0 6 56 5 1 85 83 0.0 6 81 2 86 101 0.0 6 94 4 2 0 1 87 45 0.0 6 39 4 1 1 88 54 0.0 6 52 2 89 54 0.0 6 50 3 0 0 1 90 68 0.0 6 63 4 0 0 0 1 91 46 0.0 6 46 92 73 0.0 6 66 6 0 0 0 1 93 95 0.0 6 91 3 1 94 100 0.0 6 97 3 95 72 0.0 6 71 0 1 96 99 0.0 6 94 5 97 79 0.0 6 70 7 1 0 0 0 1 98 92 0.0 6 86 5 0 1 99 111 0.0 6 107 4 100 90 0.0 6 86 0 1 0 2 1 101 92 0.0 6 90 1 1 102 71 0.0 6 67 3 1 103 100 0.0 6 95 2 1 1 1 104 118 0.0 6 111 2 2 1 2 105 106 0.0 6 100 5 1 106 107 0.0 6 101 5 1 107 181 0.0 6 172 7 1 0 0 1 108 126 0.0 6 121 3 2 109 130 0.0 6 127 2 1 110 135 0.0 6 128 7 111 147 0.0 6 138 7 0 0 0 0 2 112 126 0.0 6 119 5 1 0 0 1 113 175 0.0 6 167 6 1 0 0 0 1 114 170 0.0 6 161 8 0 0 0 0 1 115 232 0.0 6 228 3 1 116 158 0.0 6 154 3 1 117 159 0.0 6 155 3 0 0 1 118 192 0.0 6 188 3 0 1 119 172 0.0 6 168 4 120 207 0.0 6 204 2 1 121 250 0.0 6 241 8 1 122 200 0.0 6 197 2 1 123 206 0.0 6 199 5 2 124 222 0.0 6 215 4 1 1 0 0 1 125 202 0.0 6 198 4 126 277 0.0 6 268 4 2 2 0 0 1 127 301 0.0 6 293 7 0 0 1 128 342 0.0 6 332 9 1 129 297 0.0 6 288 8 0 0 0 1 130 335 0.0 6 328 6 0 0 0 1 131 392 0.0 6 381 10 1 132 362 0.0 6 352 7 2 0 0 0 1 133 454 0.0 6 435 15 1 0 3 134 461 0.0 6 452 8 0 0 0 0 1 135 500 0.0 6 491 7 1 0 1 136 543 0.0 6 528 12 1 0 1 0 1 137 581 0.0 6 570 9 1 0 1 138 548 0.0 6 538 8 1 0 0 1 139 560 0.0 6 548 10 1 0 1 140 612 0.0 6 597 11 1 0 0 3 141 698 0.0 6 687 9 1 0 0 0 1 142 928 0.0 6 916 9 1 0 0 1 1 143 750 0.0 6 739 10 0 1 144 868 0.0 6 848 17 1 0 1 0 1 145 1084 0.0 6 1068 10 1 1 2 0 2 146 1166 0.0 6 1151 10 3 0 1 1 147 1059 0.0 6 1043 12 3 0 0 0 1 148 1297 0.0 6 1273 17 3 0 1 2 1 149 1107 0.0 6 1093 12 1 1 150 1094 0.0 6 1078 12 2 0 2 151 1287 0.0 6 1263 17 4 0 2 0 1 152 1423 0.0 6 1401 18 1 1 0 2 153 1811 0.0 6 1765 35 4 0 3 0 4 154 1393 0.0 6 1366 22 0 2 2 0 1 155 1712 0.0 6 1686 23 1 1 1 156 1594 0.0 6 1579 11 2 1 1 157 1581 0.0 6 1563 15 1 0 1 0 1 158 1598 0.0 6 1580 16 0 0 1 0 1 159 1402 0.0 6 1385 15 0 0 1 1 160 1437 0.0 6 1419 13 4 0 0 0 1 161 1400 0.0 6 1380 18 1 0 1 162 1295 0.0 6 1283 11 1 163 1374 0.0 6 1359 10 2 2 1 164 1123 0.0 6 1102 14 6 0 1 165 1047 0.0 6 1033 9 3 2 166 1072 0.0 6 1053 15 1 0 0 1 2 167 1054 0.0 6 1042 10 1 0 1 168 973 0.0 6 962 8 1 1 0 0 1 169 1148 0.0 6 1133 13 1 1 170 1118 0.0 6 1105 12 0 0 0 1 171 1415 0.0 6 1404 8 2 0 1 172 1106 0.0 6 1094 11 0 1 173 1177 0.0 6 1166 10 1 174 1255 0.0 6 1236 15 2 0 2 175 1296 0.0 6 1284 9 1 0 1 1 176 1148 0.0 6 1140 5 0 0 0 3 177 1071 0.0 6 1059 9 1 0 2 178 1101 0.0 6 1091 10 179 1132 0.0 6 1122 9 0 1 180 846 0.0 6 840 6 181 873 0.0 6 864 5 1 2 0 0 1 182 683 0.0 6 674 8 0 1 183 736 0.0 6 732 4 184 665 0.0 6 656 9 185 593 0.0 6 584 7 1 0 1 186 879 0.0 6 873 5 0 1 187 1297 0.0 6 1283 10 0 3 1 188 1229 0.0 6 1217 12 189 908 0.0 6 901 4 3 190 795 0.0 6 788 4 1 0 1 1 191 724 0.0 6 721 3 192 730 0.0 6 725 5 193 682 0.0 6 676 6 194 653 0.0 6 643 9 0 0 0 0 1 195 493 0.0 6 490 3 196 556 0.0 6 551 5 197 511 0.0 6 511 198 457 0.0 6 456 0 1 199 485 0.0 6 477 6 0 1 0 0 1 200 543 0.0 6 538 5 201 408 0.0 6 401 6 1 202 359 0.0 6 352 5 2 203 284 0.0 6 274 8 0 0 1 0 1 204 283 0.0 6 275 8 205 274 0.0 6 270 4 206 286 0.0 6 281 5 207 244 0.0 6 241 3 208 332 0.0 6 327 3 2 209 229 0.0 6 224 5 210 221 0.0 6 218 3 211 246 0.0 6 243 3 212 187 0.0 6 184 2 0 1 213 166 0.0 6 164 2 214 217 0.0 6 213 3 0 0 0 1 215 228 0.0 6 220 8 216 209 0.0 6 207 2 217 262 0.0 6 258 3 1 218 129 0.0 6 129 219 128 0.0 6 126 2 220 119 0.0 6 114 4 1 221 93 0.0 6 91 2 222 66 0.0 6 65 1 223 51 0.0 6 50 1 224 100 0.0 6 96 4 225 103 0.0 6 102 1 226 95 0.0 6 94 1 227 87 0.0 6 87 228 62 0.0 6 60 2 229 63 0.0 6 61 2 230 73 0.0 6 72 1 231 83 0.0 6 75 8 232 85 0.0 6 80 5 233 39 0.0 6 39 234 48 0.0 6 44 3 0 1 235 48 0.0 6 46 2 236 22 0.0 6 22 237 21 0.0 6 21 238 16 0.0 6 15 1 239 11 0.0 6 11 240 30 0.0 6 29 1 241 30 0.0 6 30 242 21 0.0 6 21 243 9 0.0 6 9 244 16 0.0 6 16 245 12 0.0 6 12 246 10 0.0 6 10 247 16 0.0 6 16 248 8 0.0 6 7 1 249 12 0.0 6 12 250 13 0.0 6 13 251 15 0.0 6 15 252 8 0.0 6 8 253 1 0.0 6 1 254 20 0.0 6 20 255 6 0.0 6 6 256 9 0.0 6 9 257 2 0.0 6 2 259 1 0.0 6 1 260 1 0.0 6 1 264 1 0.0 6 1 279 1 0.0 6 1 280 2 0.0 6 2 281 12 0.0 6 11 1 282 90 0.0 6 89 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2352 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 50 1 0.0 6 1 69 10 0.0 6 10 70 8 0.0 6 8 71 21 0.0 6 21 72 10 0.0 6 10 73 9 0.0 6 9 74 15 0.0 6 15 75 23 0.0 6 22 1 76 28 0.0 6 27 1 77 20 0.0 6 20 78 11 0.0 6 10 1 79 17 0.0 6 16 0 0 0 1 80 12 0.0 6 12 81 22 0.0 6 21 0 0 0 1 82 17 0.0 6 17 83 9 0.0 6 9 84 8 0.0 6 8 85 8 0.0 6 8 86 25 0.0 6 25 87 29 0.0 6 28 1 88 27 0.0 6 27 89 19 0.0 6 19 90 28 0.0 6 28 91 20 0.0 6 20 92 19 0.0 6 19 93 18 0.0 6 18 94 21 0.0 6 21 95 31 0.0 6 31 96 36 0.0 6 36 97 23 0.0 6 23 98 13 0.0 6 13 99 29 0.0 6 27 1 0 1 100 23 0.0 6 23 101 32 0.0 6 32 102 27 0.0 6 27 103 41 0.0 6 40 0 1 104 37 0.0 6 37 105 29 0.0 6 29 106 9 0.0 6 9 107 32 0.0 6 32 108 19 0.0 6 19 109 21 0.0 6 21 110 1 0.0 6 1 111 6 0.0 6 5 0 0 0 1 112 24 0.0 6 24 113 14 0.0 6 14 114 5 0.0 6 5 115 20 0.0 6 20 116 18 0.0 6 17 1 117 22 0.0 6 22 118 44 0.0 6 44 119 9 0.0 6 9 120 5 0.0 6 5 121 5 0.0 6 5 122 16 0.0 6 16 123 13 0.0 6 13 124 18 0.0 6 18 125 46 0.0 6 46 126 50 0.0 6 50 127 3 0.0 6 3 128 6 0.0 6 6 129 9 0.0 6 9 130 10 0.0 6 9 0 0 0 1 131 8 0.0 6 8 132 19 0.0 6 19 133 22 0.0 6 22 134 33 0.0 6 33 135 9 0.0 6 9 136 18 0.0 6 18 137 19 0.0 6 19 138 25 0.0 6 25 139 5 0.0 6 5 140 6 0.0 6 6 141 12 0.0 6 11 0 0 0 1 142 19 0.0 6 18 1 143 18 0.0 6 17 1 144 36 0.0 6 36 145 11 0.0 6 11 146 50 0.0 6 50 147 5 0.0 6 5 148 21 0.0 6 20 0 0 0 1 149 10 0.0 6 9 0 0 1 150 8 0.0 6 8 151 25 0.0 6 25 152 7 0.0 6 4 2 0 0 1 153 11 0.0 6 11 154 20 0.0 6 20 155 13 0.0 6 12 1 156 25 0.0 6 25 157 1 0.0 6 1 158 5 0.0 6 4 1 159 12 0.0 6 12 160 9 0.0 6 9 161 54 0.0 6 54 162 7 0.0 6 7 163 15 0.0 6 15 164 16 0.0 6 16 165 7 0.0 6 7 166 7 0.0 6 7 167 16 0.0 6 16 168 9 0.0 6 9 169 19 0.0 6 19 170 4 0.0 6 4 171 10 0.0 6 9 0 0 1 172 18 0.0 6 18 173 7 0.0 6 7 174 24 0.0 6 24 175 42 0.0 6 42 176 21 0.0 6 21 177 32 0.0 6 32 178 7 0.0 6 7 179 37 0.0 6 37 180 41 0.0 6 41 181 13 0.0 6 13 182 9 0.0 6 9 183 12 0.0 6 12 184 8 0.0 6 8 185 22 0.0 6 21 1 186 10 0.0 6 10 187 10 0.0 6 9 1 188 13 0.0 6 13 189 5 0.0 6 5 190 10 0.0 6 10 191 9 0.0 6 9 192 5 0.0 6 5 193 4 0.0 6 4 194 10 0.0 6 10 195 11 0.0 6 11 196 4 0.0 6 2 0 2 197 7 0.0 6 6 1 198 52 0.0 6 52 199 1 0.0 6 1 200 2 0.0 6 2 201 2 0.0 6 1 0 1 203 4 0.0 6 4 204 8 0.0 6 8 205 1 0.0 6 1 206 1 0.0 6 1 207 1 0.0 6 1 209 4 0.0 6 4 210 1 0.0 6 1 211 2 0.0 6 2 212 3 0.0 6 3 213 1 0.0 6 1 214 4 0.0 6 4 215 1 0.0 6 1 221 1 0.0 6 1 222 5 0.0 6 5 224 3 0.0 6 3 225 4 0.0 6 4 227 1 0.0 6 1 229 1 0.0 6 1 230 2 0.0 6 2 241 1 0.0 6 1 242 7 0.0 6 7 271 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_10_181_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_10_408_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_10_181_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_10_408_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.81 s (76 µs/read; 0.79 M reads/minute). === Summary === Total read pairs processed: 76,553 Read 1 with adapter: 76,542 (100.0%) Read 2 with adapter: 76,524 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 40 (0.1%) Pairs written (passing filters): 76,513 (99.9%) Total basepairs processed: 39,551,590 bp Read 1: 22,411,714 bp Read 2: 17,139,876 bp Total written (filtered): 4,899,951 bp (12.4%) Read 1: 2,450,602 bp Read 2: 2,449,349 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 76496 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 2 299.0 1 0 2 5 1 74.8 1 1 6 1 18.7 1 0 0 1 9 1 0.3 2 1 15 2 0.0 4 2 16 1 0.0 4 1 20 9 0.0 6 8 1 21 9 0.0 6 7 2 22 2 0.0 6 2 23 1 0.0 6 1 24 5 0.0 6 4 0 1 25 5 0.0 6 5 26 6 0.0 6 6 27 5 0.0 6 5 28 2 0.0 6 2 29 2 0.0 6 2 30 1 0.0 6 1 31 1 0.0 6 1 32 2 0.0 6 1 1 33 2 0.0 6 2 34 4 0.0 6 3 1 35 2 0.0 6 2 38 5 0.0 6 5 39 2 0.0 6 0 2 41 2 0.0 6 1 1 42 6 0.0 6 6 43 1 0.0 6 1 44 5 0.0 6 5 45 3 0.0 6 3 46 3 0.0 6 3 47 2 0.0 6 2 48 2 0.0 6 2 49 2 0.0 6 0 2 50 2 0.0 6 2 53 4 0.0 6 4 55 1 0.0 6 1 56 5 0.0 6 4 1 57 3 0.0 6 3 58 1 0.0 6 1 59 1 0.0 6 1 60 2 0.0 6 2 61 1 0.0 6 1 62 3 0.0 6 3 63 1 0.0 6 1 64 9 0.0 6 8 1 65 5 0.0 6 4 1 66 1 0.0 6 0 1 67 6 0.0 6 6 68 1 0.0 6 1 72 1 0.0 6 1 73 2 0.0 6 2 74 1 0.0 6 1 75 1 0.0 6 1 77 2 0.0 6 2 78 2 0.0 6 2 80 1 0.0 6 1 81 1 0.0 6 1 82 1 0.0 6 1 84 1 0.0 6 1 85 2 0.0 6 2 86 12 0.0 6 11 1 87 5 0.0 6 5 89 6 0.0 6 6 90 2 0.0 6 2 91 3 0.0 6 2 1 92 4 0.0 6 4 93 5 0.0 6 4 0 1 94 1 0.0 6 1 95 1 0.0 6 1 96 1 0.0 6 1 97 3 0.0 6 3 98 10 0.0 6 9 1 99 2 0.0 6 2 100 4 0.0 6 4 101 5 0.0 6 3 2 102 6 0.0 6 6 103 5 0.0 6 5 104 2 0.0 6 2 105 6 0.0 6 6 106 11 0.0 6 8 2 0 1 107 5 0.0 6 5 108 17 0.0 6 13 4 109 4 0.0 6 4 110 3 0.0 6 3 111 8 0.0 6 7 1 112 10 0.0 6 9 1 113 3 0.0 6 3 114 9 0.0 6 8 1 115 11 0.0 6 11 116 6 0.0 6 5 1 117 8 0.0 6 7 1 118 3 0.0 6 3 119 14 0.0 6 14 120 1 0.0 6 1 121 7 0.0 6 6 1 122 17 0.0 6 15 1 1 123 13 0.0 6 13 124 14 0.0 6 13 1 125 14 0.0 6 13 1 126 8 0.0 6 8 127 10 0.0 6 10 128 31 0.0 6 28 2 1 129 9 0.0 6 8 1 130 8 0.0 6 8 131 12 0.0 6 12 132 31 0.0 6 30 1 133 12 0.0 6 11 1 134 17 0.0 6 16 0 0 1 135 16 0.0 6 16 136 19 0.0 6 18 1 137 17 0.0 6 17 138 20 0.0 6 19 1 139 14 0.0 6 14 140 11 0.0 6 11 141 4 0.0 6 4 142 10 0.0 6 10 143 42 0.0 6 42 144 51 0.0 6 51 145 51 0.0 6 51 146 59 0.0 6 59 147 33 0.0 6 33 148 64 0.0 6 63 1 149 92 0.0 6 92 150 65 0.0 6 65 151 63 0.0 6 62 1 152 84 0.0 6 83 1 153 109 0.0 6 108 1 154 45 0.0 6 44 1 155 31 0.0 6 31 156 43 0.0 6 42 1 157 47 0.0 6 47 158 40 0.0 6 40 159 36 0.0 6 35 1 160 47 0.0 6 47 161 64 0.0 6 64 162 48 0.0 6 48 163 55 0.0 6 55 164 60 0.0 6 60 165 45 0.0 6 45 166 27 0.0 6 26 1 167 54 0.0 6 54 168 60 0.0 6 59 1 169 69 0.0 6 67 2 170 87 0.0 6 84 3 171 51 0.0 6 50 1 172 24 0.0 6 23 1 173 53 0.0 6 53 174 62 0.0 6 61 1 175 38 0.0 6 36 1 1 176 38 0.0 6 38 177 24 0.0 6 24 178 38 0.0 6 35 2 1 179 25 0.0 6 24 1 180 41 0.0 6 41 181 18 0.0 6 18 182 38 0.0 6 37 1 183 61 0.0 6 60 1 184 40 0.0 6 39 1 185 44 0.0 6 41 3 186 32 0.0 6 30 2 187 41 0.0 6 38 3 188 84 0.0 6 82 2 189 35 0.0 6 32 3 190 36 0.0 6 36 191 76 0.0 6 71 5 192 79 0.0 6 78 1 193 75 0.0 6 71 4 194 132 0.0 6 131 1 195 115 0.0 6 112 3 196 102 0.0 6 100 1 1 197 122 0.0 6 119 3 198 74 0.0 6 74 199 78 0.0 6 77 1 200 72 0.0 6 71 1 201 62 0.0 6 61 1 202 35 0.0 6 32 3 203 112 0.0 6 109 3 204 101 0.0 6 98 3 205 143 0.0 6 140 3 206 81 0.0 6 78 3 207 112 0.0 6 112 208 161 0.0 6 155 6 209 328 0.0 6 317 11 210 264 0.0 6 248 15 1 211 591 0.0 6 574 17 212 364 0.0 6 355 9 213 267 0.0 6 261 6 214 641 0.0 6 631 9 0 0 1 215 136 0.0 6 124 12 216 623 0.0 6 613 10 217 124 0.0 6 120 4 218 170 0.0 6 166 4 219 107 0.0 6 101 6 220 154 0.0 6 151 3 221 91 0.0 6 86 5 222 253 0.0 6 251 2 223 118 0.0 6 113 5 224 257 0.0 6 251 6 225 175 0.0 6 163 12 226 715 0.0 6 693 21 1 227 390 0.0 6 381 9 228 253 0.0 6 244 9 229 381 0.0 6 370 11 230 277 0.0 6 271 6 231 305 0.0 6 303 2 232 214 0.0 6 210 4 233 163 0.0 6 162 1 234 164 0.0 6 161 3 235 105 0.0 6 103 1 0 1 236 164 0.0 6 160 4 237 199 0.0 6 197 2 238 128 0.0 6 124 3 1 239 273 0.0 6 260 13 240 720 0.0 6 715 5 241 158 0.0 6 152 6 242 157 0.0 6 156 1 243 137 0.0 6 132 5 244 94 0.0 6 91 3 245 92 0.0 6 90 2 246 206 0.0 6 196 10 247 397 0.0 6 386 11 248 240 0.0 6 235 5 249 217 0.0 6 210 7 250 562 0.0 6 548 14 251 325 0.0 6 296 29 252 1337 0.0 6 1317 19 0 0 1 253 417 0.0 6 401 15 1 254 274 0.0 6 265 9 255 426 0.0 6 412 13 1 256 619 0.0 6 602 17 257 852 0.0 6 814 36 1 1 258 741 0.0 6 701 38 1 1 259 1273 0.0 6 1242 27 2 0 2 260 505 0.0 6 481 23 1 261 946 0.0 6 929 17 262 496 0.0 6 467 29 263 1064 0.0 6 1046 18 264 502 0.0 6 492 10 265 320 0.0 6 302 13 5 266 762 0.0 6 735 25 1 1 267 994 0.0 6 966 28 268 388 0.0 6 374 13 1 269 895 0.0 6 865 30 270 550 0.0 6 542 7 1 271 456 0.0 6 442 14 272 344 0.0 6 338 6 273 392 0.0 6 386 6 274 421 0.0 6 408 13 275 878 0.0 6 855 21 2 276 3635 0.0 6 3562 67 4 0 1 1 277 2063 0.0 6 1985 72 6 278 2255 0.0 6 2036 212 6 0 1 279 36170 0.0 6 35637 519 12 1 0 0 1 280 14 0.0 6 11 0 3 281 5 0.0 6 4 0 1 282 1 0.0 6 0 0 0 0 1 283 2 0.0 6 1 0 0 0 1 285 2 0.0 6 2 286 1 0.0 6 1 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 46 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 1 0.0 6 1 71 1 0.0 6 1 72 1 0.0 6 1 74 2 0.0 6 2 75 1 0.0 6 1 77 1 0.0 6 1 79 3 0.0 6 3 80 1 0.0 6 1 81 1 0.0 6 1 82 1 0.0 6 1 83 1 0.0 6 1 85 1 0.0 6 1 86 1 0.0 6 1 87 2 0.0 6 2 88 1 0.0 6 1 89 1 0.0 6 1 93 1 0.0 6 1 98 1 0.0 6 1 102 1 0.0 6 1 103 2 0.0 6 2 106 1 0.0 6 1 108 2 0.0 6 2 109 2 0.0 6 2 112 1 0.0 6 1 121 1 0.0 6 1 122 1 0.0 6 1 139 1 0.0 6 1 149 1 0.0 6 1 150 1 0.0 6 1 152 1 0.0 6 1 157 1 0.0 6 1 170 2 0.0 6 2 181 1 0.0 6 1 183 2 0.0 6 2 191 1 0.0 6 1 200 1 0.0 6 1 207 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 76477 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1196.1 0 0 2 5 1 74.8 1 1 6 1 18.7 1 1 10 1 0.1 3 0 1 11 2 0.0 3 2 12 1 0.0 3 0 1 13 10 0.0 3 10 14 3 0.0 4 2 1 15 1 0.0 4 1 16 15 0.0 4 12 3 17 7 0.0 5 5 1 1 18 12 0.0 5 12 19 11 0.0 5 10 1 20 28 0.0 6 28 21 34 0.0 6 34 22 32 0.0 6 31 1 23 19 0.0 6 19 24 15 0.0 6 15 25 18 0.0 6 18 26 14 0.0 6 13 1 27 13 0.0 6 12 1 28 9 0.0 6 8 0 1 29 16 0.0 6 15 1 30 12 0.0 6 11 1 31 17 0.0 6 15 2 32 10 0.0 6 9 0 1 33 8 0.0 6 8 34 12 0.0 6 11 1 35 12 0.0 6 8 3 1 36 4 0.0 6 4 37 8 0.0 6 8 38 2 0.0 6 2 39 3 0.0 6 3 40 6 0.0 6 6 41 13 0.0 6 13 42 3 0.0 6 2 1 43 6 0.0 6 5 0 1 44 10 0.0 6 9 0 0 0 1 45 11 0.0 6 8 3 46 11 0.0 6 10 0 0 0 1 47 22 0.0 6 21 1 48 9 0.0 6 9 49 5 0.0 6 5 50 14 0.0 6 13 1 51 5 0.0 6 5 52 24 0.0 6 20 4 53 17 0.0 6 12 5 54 24 0.0 6 18 4 0 0 1 0 1 55 16 0.0 6 14 2 56 23 0.0 6 22 1 57 21 0.0 6 18 3 58 11 0.0 6 9 1 0 0 1 59 10 0.0 6 8 2 60 18 0.0 6 15 3 61 14 0.0 6 12 2 62 10 0.0 6 8 2 63 9 0.0 6 9 64 14 0.0 6 14 65 10 0.0 6 10 66 29 0.0 6 28 1 67 158 0.0 6 150 7 0 1 68 22 0.0 6 19 3 69 49 0.0 6 48 1 70 14 0.0 6 12 2 71 28 0.0 6 26 0 1 0 0 1 72 21 0.0 6 19 2 73 5 0.0 6 5 74 8 0.0 6 7 1 75 14 0.0 6 13 1 76 23 0.0 6 22 1 77 12 0.0 6 11 0 0 0 1 78 18 0.0 6 17 1 79 18 0.0 6 15 3 80 13 0.0 6 12 1 81 18 0.0 6 18 82 26 0.0 6 26 83 73 0.0 6 66 4 1 1 1 84 39 0.0 6 36 3 85 39 0.0 6 33 3 1 0 2 86 22 0.0 6 21 1 87 36 0.0 6 34 2 88 21 0.0 6 19 2 89 32 0.0 6 32 90 39 0.0 6 37 2 91 35 0.0 6 30 5 92 13 0.0 6 13 93 48 0.0 6 46 2 94 65 0.0 6 59 5 1 95 34 0.0 6 28 5 0 0 1 96 34 0.0 6 30 4 97 34 0.0 6 32 2 98 27 0.0 6 22 5 99 47 0.0 6 45 2 100 52 0.0 6 45 6 0 1 101 37 0.0 6 33 4 102 58 0.0 6 52 6 103 71 0.0 6 69 2 104 70 0.0 6 62 6 2 105 61 0.0 6 54 7 106 43 0.0 6 43 107 43 0.0 6 39 3 1 108 34 0.0 6 34 109 54 0.0 6 51 3 110 45 0.0 6 39 4 0 0 2 111 37 0.0 6 34 3 112 34 0.0 6 32 1 0 0 0 0 1 113 35 0.0 6 34 1 114 65 0.0 6 58 7 115 41 0.0 6 38 2 0 1 116 60 0.0 6 54 5 0 1 117 37 0.0 6 35 2 118 46 0.0 6 44 2 119 54 0.0 6 49 5 120 61 0.0 6 59 2 121 42 0.0 6 40 2 122 56 0.0 6 53 3 123 59 0.0 6 57 2 124 82 0.0 6 78 3 1 125 96 0.0 6 93 3 126 72 0.0 6 69 2 1 127 92 0.0 6 85 7 128 55 0.0 6 55 129 44 0.0 6 42 2 130 55 0.0 6 52 2 1 131 120 0.0 6 114 6 132 121 0.0 6 117 4 133 98 0.0 6 94 3 0 1 134 185 0.0 6 174 7 1 1 2 135 233 0.0 6 230 3 136 108 0.0 6 106 2 137 180 0.0 6 175 5 138 70 0.0 6 66 4 139 158 0.0 6 145 11 2 140 256 0.0 6 248 6 1 0 1 141 216 0.0 6 208 7 0 0 0 1 142 313 0.0 6 301 10 1 0 1 143 274 0.0 6 266 8 144 409 0.0 6 398 10 1 145 196 0.0 6 187 9 146 326 0.0 6 316 10 147 194 0.0 6 186 7 1 148 258 0.0 6 254 4 149 422 0.0 6 411 7 2 0 2 150 739 0.0 6 709 28 0 0 2 151 353 0.0 6 344 9 152 462 0.0 6 447 14 0 1 153 394 0.0 6 381 12 1 154 276 0.0 6 271 5 155 199 0.0 6 190 7 2 156 228 0.0 6 221 5 1 0 1 157 361 0.0 6 350 11 158 421 0.0 6 407 13 0 1 159 206 0.0 6 198 6 1 0 1 160 340 0.0 6 332 7 0 0 1 161 214 0.0 6 209 3 0 1 1 162 137 0.0 6 137 163 358 0.0 6 345 12 0 0 1 164 371 0.0 6 361 10 165 410 0.0 6 398 11 1 166 629 0.0 6 615 11 3 167 450 0.0 6 434 13 0 0 3 168 688 0.0 6 671 16 0 1 169 369 0.0 6 350 16 2 1 170 507 0.0 6 491 16 171 745 0.0 6 721 24 172 316 0.0 6 309 5 0 0 2 173 382 0.0 6 371 11 174 581 0.0 6 563 17 1 175 725 0.0 6 698 24 1 0 2 176 631 0.0 6 608 21 2 177 733 0.0 6 712 21 178 585 0.0 6 563 20 2 179 556 0.0 6 531 24 0 0 1 180 681 0.0 6 648 31 1 0 1 181 666 0.0 6 648 17 1 182 149 0.0 6 142 7 183 641 0.0 6 624 17 184 728 0.0 6 707 18 1 0 1 0 1 185 813 0.0 6 786 22 3 1 1 186 1279 0.0 6 1255 21 3 187 1038 0.0 6 1011 25 0 1 1 188 1102 0.0 6 1074 27 0 1 189 1318 0.0 6 1280 35 2 0 1 190 1447 0.0 6 1399 43 0 3 1 1 191 2050 0.0 6 1997 49 2 1 1 192 787 0.0 6 749 37 1 193 1513 0.0 6 1461 51 1 194 1164 0.0 6 1137 22 4 0 1 195 955 0.0 6 917 36 2 196 2010 0.0 6 1978 30 1 0 1 197 668 0.0 6 640 25 3 198 1955 0.0 6 1913 40 0 0 1 1 199 668 0.0 6 653 14 1 200 1188 0.0 6 1155 32 1 201 1305 0.0 6 1270 33 2 202 1391 0.0 6 1359 32 203 825 0.0 6 800 21 2 1 1 204 1078 0.0 6 1052 25 1 205 990 0.0 6 975 12 3 206 840 0.0 6 818 20 2 207 1093 0.0 6 1057 35 1 208 1604 0.0 6 1564 39 0 1 209 114 0.0 6 110 4 210 466 0.0 6 444 22 211 1102 0.0 6 1074 27 1 212 763 0.0 6 752 8 1 2 213 415 0.0 6 400 13 1 0 1 214 918 0.0 6 896 18 3 0 0 1 215 986 0.0 6 964 21 1 216 1244 0.0 6 1212 31 0 0 1 217 645 0.0 6 621 23 1 218 463 0.0 6 451 12 219 354 0.0 6 350 4 220 230 0.0 6 221 7 2 221 414 0.0 6 405 9 222 503 0.0 6 493 9 0 0 0 0 1 223 275 0.0 6 268 7 224 262 0.0 6 257 5 225 277 0.0 6 270 6 0 0 0 1 226 498 0.0 6 478 18 2 227 1131 0.0 6 1100 29 1 1 228 1090 0.0 6 1054 35 0 1 229 1277 0.0 6 1216 59 0 1 1 230 3139 0.0 6 3074 64 1 231 645 0.0 6 624 21 232 1585 0.0 6 1551 32 0 1 1 233 164 0.0 6 159 5 234 460 0.0 6 447 11 1 0 1 235 263 0.0 6 258 3 1 0 1 236 262 0.0 6 258 4 237 257 0.0 6 248 8 0 1 238 1058 0.0 6 1031 26 1 239 99 0.0 6 99 240 94 0.0 6 92 2 241 226 0.0 6 222 4 242 145 0.0 6 143 2 243 41 0.0 6 38 2 1 244 74 0.0 6 70 4 245 346 0.0 6 335 10 0 1 246 213 0.0 6 211 2 247 196 0.0 6 193 2 1 248 141 0.0 6 135 6 249 79 0.0 6 77 2 250 70 0.0 6 66 3 1 251 277 0.0 6 273 4 252 42 0.0 6 39 2 1 253 30 0.0 6 25 4 0 0 1 254 31 0.0 6 30 1 255 13 0.0 6 13 256 4 0.0 6 3 0 0 1 257 2 0.0 6 2 258 1 0.0 6 1 259 4 0.0 6 4 260 1 0.0 6 1 263 2 0.0 6 2 265 1 0.0 6 1 270 1 0.0 6 1 277 1 0.0 6 1 278 35 0.0 6 34 1 279 103 0.0 6 98 4 1 280 2 0.0 6 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 47 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 73 2 0.0 6 2 75 2 0.0 6 2 77 2 0.0 6 2 78 2 0.0 6 2 79 1 0.0 6 1 80 2 0.0 6 2 81 3 0.0 6 3 82 2 0.0 6 2 83 1 0.0 6 1 85 2 0.0 6 2 87 1 0.0 6 1 90 3 0.0 6 3 91 1 0.0 6 1 92 1 0.0 6 1 96 2 0.0 6 2 97 1 0.0 6 1 98 3 0.0 6 3 99 1 0.0 6 1 104 1 0.0 6 1 107 1 0.0 6 1 109 1 0.0 6 1 114 1 0.0 6 1 120 1 0.0 6 1 121 1 0.0 6 1 134 1 0.0 6 1 145 1 0.0 6 1 149 1 0.0 6 1 151 1 0.0 6 1 155 1 0.0 6 1 160 1 0.0 6 1 162 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_11_182_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_11_409_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_11_182_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_11_409_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.42 s (63 µs/read; 0.95 M reads/minute). === Summary === Total read pairs processed: 54,212 Read 1 with adapter: 54,207 (100.0%) Read 2 with adapter: 54,195 (100.0%) == Read fate breakdown == Pairs that were too short: 9 (0.0%) Pairs discarded as untrimmed: 22 (0.0%) Pairs written (passing filters): 54,181 (99.9%) Total basepairs processed: 24,239,995 bp Read 1: 13,490,074 bp Read 2: 10,749,921 bp Total written (filtered): 3,401,254 bp (14.0%) Read 1: 1,789,884 bp Read 2: 1,611,370 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 53141 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 847.1 0 2 1 4 2 211.8 1 0 2 6 2 13.2 1 0 0 2 7 2 3.3 2 2 9 1 0.2 2 1 15 1 0.0 4 1 16 2 0.0 4 2 17 3 0.0 5 1 2 18 1 0.0 5 1 19 1 0.0 5 1 20 50 0.0 6 48 2 21 27 0.0 6 26 1 22 27 0.0 6 26 1 23 7 0.0 6 7 24 6 0.0 6 6 25 10 0.0 6 10 26 8 0.0 6 8 27 9 0.0 6 9 28 7 0.0 6 7 29 7 0.0 6 7 30 4 0.0 6 4 31 3 0.0 6 3 33 6 0.0 6 6 34 6 0.0 6 6 35 1 0.0 6 1 36 6 0.0 6 6 37 5 0.0 6 5 38 4 0.0 6 4 39 7 0.0 6 6 0 0 0 1 40 4 0.0 6 4 41 7 0.0 6 7 42 5 0.0 6 5 43 3 0.0 6 2 1 44 5 0.0 6 4 1 45 1 0.0 6 1 46 3 0.0 6 2 1 47 2 0.0 6 2 48 1 0.0 6 0 0 0 0 0 0 1 49 4 0.0 6 4 50 2 0.0 6 2 51 2 0.0 6 2 52 1 0.0 6 1 53 7 0.0 6 7 54 1 0.0 6 1 55 3 0.0 6 3 56 2 0.0 6 2 57 2 0.0 6 2 58 1 0.0 6 1 59 2 0.0 6 2 60 2 0.0 6 2 61 2 0.0 6 2 62 2 0.0 6 2 63 9 0.0 6 9 64 4 0.0 6 4 65 2 0.0 6 2 67 7 0.0 6 5 1 1 68 2 0.0 6 2 69 6 0.0 6 6 70 1 0.0 6 1 71 4 0.0 6 4 72 7 0.0 6 7 73 6 0.0 6 5 1 74 4 0.0 6 4 75 4 0.0 6 4 76 6 0.0 6 6 77 5 0.0 6 4 1 78 3 0.0 6 3 79 5 0.0 6 5 80 5 0.0 6 5 81 6 0.0 6 6 82 1 0.0 6 1 83 3 0.0 6 3 84 8 0.0 6 6 2 85 3 0.0 6 3 86 9 0.0 6 9 87 6 0.0 6 5 0 0 1 88 2 0.0 6 2 89 7 0.0 6 7 90 3 0.0 6 3 91 4 0.0 6 4 92 6 0.0 6 5 1 93 8 0.0 6 7 0 0 0 0 0 1 94 4 0.0 6 4 95 5 0.0 6 5 96 9 0.0 6 7 2 97 9 0.0 6 8 1 98 10 0.0 6 10 99 9 0.0 6 8 1 100 4 0.0 6 3 1 101 3 0.0 6 3 102 9 0.0 6 9 103 11 0.0 6 10 1 104 8 0.0 6 8 105 7 0.0 6 7 106 11 0.0 6 11 107 17 0.0 6 17 108 14 0.0 6 13 1 109 4 0.0 6 4 110 6 0.0 6 6 111 8 0.0 6 8 112 19 0.0 6 18 1 113 18 0.0 6 18 114 16 0.0 6 15 1 115 15 0.0 6 13 1 1 116 19 0.0 6 19 117 14 0.0 6 14 118 9 0.0 6 9 119 26 0.0 6 24 2 120 9 0.0 6 9 121 14 0.0 6 13 0 1 122 24 0.0 6 24 123 12 0.0 6 12 124 15 0.0 6 15 125 16 0.0 6 16 126 16 0.0 6 16 127 20 0.0 6 20 128 16 0.0 6 15 1 129 28 0.0 6 27 1 130 19 0.0 6 18 1 131 21 0.0 6 21 132 29 0.0 6 27 2 133 21 0.0 6 20 1 134 28 0.0 6 28 135 23 0.0 6 23 136 34 0.0 6 33 1 137 26 0.0 6 26 138 31 0.0 6 31 139 48 0.0 6 48 140 61 0.0 6 59 2 141 128 0.0 6 128 142 221 0.0 6 218 3 143 315 0.0 6 313 2 144 346 0.0 6 345 1 145 288 0.0 6 287 0 0 1 146 357 0.0 6 357 147 376 0.0 6 376 148 243 0.0 6 240 3 149 547 0.0 6 545 2 150 356 0.0 6 355 0 1 151 296 0.0 6 295 1 152 330 0.0 6 329 1 153 282 0.0 6 281 0 0 0 0 1 154 364 0.0 6 364 155 426 0.0 6 425 1 156 464 0.0 6 463 1 157 202 0.0 6 202 158 212 0.0 6 212 159 290 0.0 6 290 160 418 0.0 6 418 161 105 0.0 6 104 1 162 267 0.0 6 266 1 163 352 0.0 6 352 164 240 0.0 6 238 2 165 419 0.0 6 418 1 166 157 0.0 6 155 1 0 0 1 167 181 0.0 6 180 1 168 282 0.0 6 281 1 169 426 0.0 6 426 170 502 0.0 6 501 1 171 221 0.0 6 221 172 249 0.0 6 249 173 268 0.0 6 267 1 174 257 0.0 6 256 1 175 188 0.0 6 186 1 0 0 0 1 176 106 0.0 6 105 1 177 94 0.0 6 94 178 378 0.0 6 378 179 209 0.0 6 208 1 180 128 0.0 6 127 0 1 181 122 0.0 6 121 1 182 115 0.0 6 112 3 183 193 0.0 6 193 184 228 0.0 6 228 185 249 0.0 6 247 1 0 0 1 186 554 0.0 6 552 1 1 187 270 0.0 6 270 188 369 0.0 6 369 189 284 0.0 6 283 1 190 770 0.0 6 768 2 191 252 0.0 6 252 192 528 0.0 6 525 3 193 315 0.0 6 314 1 194 485 0.0 6 481 4 195 426 0.0 6 422 4 196 386 0.0 6 384 2 197 338 0.0 6 333 5 198 286 0.0 6 283 2 0 0 0 1 199 330 0.0 6 322 7 0 0 0 1 200 263 0.0 6 260 3 201 397 0.0 6 395 1 0 0 0 1 202 734 0.0 6 728 5 1 203 302 0.0 6 299 3 204 261 0.0 6 256 4 0 0 0 0 1 205 446 0.0 6 442 4 206 475 0.0 6 468 7 207 390 0.0 6 387 3 208 425 0.0 6 422 3 209 384 0.0 6 384 210 334 0.0 6 333 0 0 0 0 0 1 211 735 0.0 6 730 4 1 212 273 0.0 6 273 213 368 0.0 6 366 2 214 352 0.0 6 351 1 215 383 0.0 6 382 1 216 398 0.0 6 394 3 0 0 0 1 217 392 0.0 6 391 1 218 396 0.0 6 393 3 219 521 0.0 6 519 2 220 373 0.0 6 370 3 221 327 0.0 6 325 2 222 418 0.0 6 411 6 1 223 309 0.0 6 307 2 224 381 0.0 6 381 225 531 0.0 6 529 1 0 0 0 1 226 789 0.0 6 787 2 227 364 0.0 6 358 6 228 476 0.0 6 473 2 1 229 345 0.0 6 343 2 230 300 0.0 6 298 2 231 290 0.0 6 289 1 232 253 0.0 6 250 3 233 263 0.0 6 260 3 234 336 0.0 6 335 1 235 326 0.0 6 326 236 346 0.0 6 346 237 233 0.0 6 232 1 238 239 0.0 6 238 1 239 306 0.0 6 304 2 240 329 0.0 6 327 2 241 430 0.0 6 425 5 242 302 0.0 6 296 6 243 287 0.0 6 284 3 244 209 0.0 6 204 5 245 269 0.0 6 265 4 246 151 0.0 6 148 3 247 165 0.0 6 161 4 248 142 0.0 6 140 2 249 271 0.0 6 268 3 250 153 0.0 6 151 2 251 178 0.0 6 175 3 252 178 0.0 6 175 2 1 253 159 0.0 6 158 0 0 0 0 1 254 241 0.0 6 238 3 255 147 0.0 6 145 2 256 140 0.0 6 136 3 0 1 257 182 0.0 6 180 2 258 177 0.0 6 175 1 1 259 259 0.0 6 255 4 260 273 0.0 6 269 4 261 114 0.0 6 113 1 262 132 0.0 6 129 3 263 255 0.0 6 250 4 1 264 120 0.0 6 117 3 265 149 0.0 6 146 3 266 82 0.0 6 82 267 140 0.0 6 140 268 149 0.0 6 145 4 269 222 0.0 6 218 4 270 91 0.0 6 91 271 151 0.0 6 146 5 272 326 0.0 6 319 7 273 204 0.0 6 200 4 274 471 0.0 6 466 5 275 220 0.0 6 217 2 0 1 276 483 0.0 6 471 12 277 1553 0.0 6 1533 19 0 1 278 889 0.0 6 877 11 1 279 8001 0.0 6 7762 226 12 1 280 87 0.0 6 74 12 1 281 10 0.0 6 10 283 1 0.0 6 1 285 1 0.0 6 1 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1066 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 1 0.0 6 1 63 4 0.0 6 4 64 21 0.0 6 21 72 1 0.0 6 1 73 4 0.0 6 4 74 6 0.0 6 6 75 12 0.0 6 12 76 12 0.0 6 12 77 9 0.0 6 9 78 14 0.0 6 14 79 5 0.0 6 5 80 10 0.0 6 10 81 20 0.0 6 20 82 7 0.0 6 7 83 10 0.0 6 10 84 11 0.0 6 11 85 15 0.0 6 15 86 15 0.0 6 15 87 10 0.0 6 10 88 17 0.0 6 17 89 4 0.0 6 4 90 9 0.0 6 9 91 10 0.0 6 10 92 13 0.0 6 13 93 4 0.0 6 4 94 8 0.0 6 8 95 14 0.0 6 14 96 10 0.0 6 10 97 12 0.0 6 12 98 1 0.0 6 1 99 9 0.0 6 9 100 11 0.0 6 11 101 14 0.0 6 14 102 16 0.0 6 15 1 103 6 0.0 6 6 104 6 0.0 6 6 105 5 0.0 6 5 106 5 0.0 6 5 107 3 0.0 6 2 1 108 1 0.0 6 1 109 3 0.0 6 3 110 10 0.0 6 10 112 2 0.0 6 2 113 1 0.0 6 1 114 2 0.0 6 2 115 3 0.0 6 3 116 6 0.0 6 6 117 5 0.0 6 5 118 13 0.0 6 13 119 10 0.0 6 10 120 7 0.0 6 7 121 7 0.0 6 7 122 15 0.0 6 15 123 7 0.0 6 7 124 19 0.0 6 19 125 7 0.0 6 7 126 11 0.0 6 11 127 6 0.0 6 6 128 7 0.0 6 7 129 2 0.0 6 2 132 3 0.0 6 3 133 15 0.0 6 15 134 17 0.0 6 17 135 6 0.0 6 6 136 3 0.0 6 3 137 5 0.0 6 5 138 6 0.0 6 6 139 2 0.0 6 2 140 2 0.0 6 2 141 2 0.0 6 2 142 7 0.0 6 7 143 33 0.0 6 33 144 2 0.0 6 2 145 1 0.0 6 1 146 1 0.0 6 1 149 2 0.0 6 2 150 5 0.0 6 5 151 7 0.0 6 7 152 7 0.0 6 7 153 4 0.0 6 4 154 8 0.0 6 8 155 4 0.0 6 4 156 1 0.0 6 1 157 17 0.0 6 17 158 32 0.0 6 32 159 5 0.0 6 5 160 22 0.0 6 22 161 12 0.0 6 12 164 1 0.0 6 1 165 4 0.0 6 4 166 5 0.0 6 5 167 5 0.0 6 5 168 2 0.0 6 2 170 7 0.0 6 7 171 15 0.0 6 15 172 6 0.0 6 6 173 17 0.0 6 17 174 7 0.0 6 7 175 2 0.0 6 2 176 1 0.0 6 1 177 3 0.0 6 3 178 6 0.0 6 6 180 3 0.0 6 3 181 12 0.0 6 12 182 1 0.0 6 1 184 9 0.0 6 9 185 6 0.0 6 6 186 2 0.0 6 2 188 1 0.0 6 1 189 4 0.0 6 4 190 7 0.0 6 7 191 5 0.0 6 5 192 3 0.0 6 3 193 4 0.0 6 4 194 1 0.0 6 1 195 5 0.0 6 5 196 2 0.0 6 2 197 1 0.0 6 0 1 198 5 0.0 6 5 199 11 0.0 6 11 200 9 0.0 6 9 201 24 0.0 6 24 202 6 0.0 6 6 203 2 0.0 6 2 204 3 0.0 6 3 205 2 0.0 6 2 206 5 0.0 6 5 207 2 0.0 6 2 208 1 0.0 6 1 209 2 0.0 6 2 210 5 0.0 6 5 211 1 0.0 6 1 212 1 0.0 6 1 213 3 0.0 6 3 215 2 0.0 6 2 216 4 0.0 6 4 217 1 0.0 6 1 218 1 0.0 6 1 219 1 0.0 6 1 220 2 0.0 6 2 221 1 0.0 6 1 223 3 0.0 6 3 224 2 0.0 6 2 225 1 0.0 6 1 227 1 0.0 6 1 230 2 0.0 6 2 232 3 0.0 6 3 233 1 0.0 6 1 238 2 0.0 6 2 242 1 0.0 6 1 244 2 0.0 6 2 248 2 0.0 6 2 249 2 0.0 6 2 250 12 0.0 6 12 251 23 0.0 6 22 1 252 3 0.0 6 3 253 3 0.0 6 3 254 1 0.0 6 1 255 1 0.0 6 1 256 2 0.0 6 2 257 3 0.0 6 3 258 3 0.0 6 3 260 1 0.0 6 1 261 2 0.0 6 2 262 1 0.0 6 1 263 1 0.0 6 1 264 1 0.0 6 1 266 1 0.0 6 1 267 3 0.0 6 3 271 2 0.0 6 2 288 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 53226 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 13.2 1 1 10 1 0.1 3 1 11 3 0.0 3 1 2 12 5 0.0 3 4 0 0 1 13 5 0.0 3 2 2 0 1 14 2 0.0 4 2 16 6 0.0 4 6 17 12 0.0 5 10 2 18 1 0.0 5 0 0 0 1 19 34 0.0 5 32 2 20 71 0.0 6 68 3 21 44 0.0 6 44 22 28 0.0 6 25 3 23 23 0.0 6 22 1 24 11 0.0 6 11 25 5 0.0 6 5 26 13 0.0 6 12 1 27 10 0.0 6 8 2 28 5 0.0 6 5 29 10 0.0 6 8 2 30 6 0.0 6 6 31 6 0.0 6 5 1 32 6 0.0 6 6 33 12 0.0 6 10 2 34 5 0.0 6 5 35 5 0.0 6 5 36 16 0.0 6 16 37 11 0.0 6 10 1 38 7 0.0 6 7 39 8 0.0 6 8 40 11 0.0 6 9 1 0 0 1 41 2 0.0 6 2 42 3 0.0 6 2 0 1 43 5 0.0 6 5 44 8 0.0 6 7 1 45 6 0.0 6 5 1 46 4 0.0 6 4 47 13 0.0 6 13 48 11 0.0 6 10 1 49 19 0.0 6 17 2 50 14 0.0 6 14 51 17 0.0 6 15 2 52 21 0.0 6 19 2 53 12 0.0 6 11 0 0 1 54 8 0.0 6 7 1 55 15 0.0 6 14 1 56 21 0.0 6 20 1 57 20 0.0 6 19 1 58 12 0.0 6 12 59 10 0.0 6 10 60 14 0.0 6 13 1 61 10 0.0 6 10 62 10 0.0 6 10 63 13 0.0 6 11 2 64 13 0.0 6 13 65 20 0.0 6 17 3 66 15 0.0 6 15 67 21 0.0 6 19 1 1 68 10 0.0 6 10 69 30 0.0 6 28 2 70 116 0.0 6 111 3 0 1 1 71 29 0.0 6 26 3 72 52 0.0 6 49 3 73 19 0.0 6 19 74 36 0.0 6 35 1 75 15 0.0 6 14 1 76 23 0.0 6 21 1 0 0 0 0 1 77 24 0.0 6 24 78 28 0.0 6 28 79 15 0.0 6 14 1 80 22 0.0 6 21 1 81 31 0.0 6 31 82 30 0.0 6 30 83 29 0.0 6 28 1 84 41 0.0 6 37 3 1 85 40 0.0 6 38 2 86 46 0.0 6 44 0 0 0 1 1 87 28 0.0 6 27 0 1 88 28 0.0 6 26 2 89 26 0.0 6 24 2 90 38 0.0 6 36 2 91 40 0.0 6 40 92 30 0.0 6 29 1 93 29 0.0 6 27 2 94 61 0.0 6 58 1 1 0 0 0 1 95 24 0.0 6 24 96 35 0.0 6 35 97 35 0.0 6 35 98 42 0.0 6 42 99 51 0.0 6 47 4 100 38 0.0 6 36 2 101 58 0.0 6 53 5 102 45 0.0 6 42 3 103 45 0.0 6 44 1 104 58 0.0 6 56 2 105 63 0.0 6 60 2 1 106 78 0.0 6 77 1 107 105 0.0 6 101 4 108 53 0.0 6 49 2 0 0 2 109 52 0.0 6 49 2 0 1 110 59 0.0 6 55 4 111 66 0.0 6 66 112 58 0.0 6 58 113 58 0.0 6 58 114 80 0.0 6 79 0 1 115 86 0.0 6 83 3 116 83 0.0 6 80 1 1 0 0 1 117 80 0.0 6 78 2 118 98 0.0 6 95 3 119 87 0.0 6 84 1 0 0 1 0 1 120 112 0.0 6 109 3 121 85 0.0 6 83 1 0 0 0 0 1 122 85 0.0 6 83 2 123 119 0.0 6 116 3 124 94 0.0 6 90 4 125 95 0.0 6 94 1 126 109 0.0 6 106 3 127 127 0.0 6 124 2 1 128 162 0.0 6 158 2 0 0 0 1 1 129 129 0.0 6 127 2 130 144 0.0 6 140 3 1 131 159 0.0 6 156 3 132 181 0.0 6 179 1 0 0 0 0 1 133 209 0.0 6 204 4 1 134 179 0.0 6 175 4 135 228 0.0 6 223 5 136 252 0.0 6 246 5 1 137 273 0.0 6 267 6 138 263 0.0 6 258 3 1 0 0 1 139 248 0.0 6 245 3 140 296 0.0 6 291 4 0 0 1 141 324 0.0 6 316 8 142 430 0.0 6 424 5 1 143 603 0.0 6 595 7 1 144 581 0.0 6 577 2 0 0 1 1 145 561 0.0 6 554 6 0 0 0 0 1 146 606 0.0 6 598 7 1 147 604 0.0 6 602 2 148 557 0.0 6 545 12 149 754 0.0 6 745 9 150 720 0.0 6 709 9 1 0 0 1 151 636 0.0 6 628 7 0 0 1 152 835 0.0 6 819 14 0 0 1 0 1 153 971 0.0 6 963 6 0 1 0 0 1 154 784 0.0 6 777 7 155 952 0.0 6 937 13 0 0 1 1 156 893 0.0 6 884 7 0 0 1 1 157 667 0.0 6 655 12 158 758 0.0 6 751 7 159 635 0.0 6 626 9 160 808 0.0 6 799 8 1 161 603 0.0 6 599 3 0 0 1 162 657 0.0 6 649 6 0 0 1 1 163 779 0.0 6 768 10 0 1 164 632 0.0 6 624 5 1 0 1 0 1 165 639 0.0 6 632 6 1 166 596 0.0 6 584 11 0 0 0 1 167 554 0.0 6 548 5 1 168 658 0.0 6 653 4 0 0 1 169 641 0.0 6 636 5 170 628 0.0 6 624 4 171 718 0.0 6 709 9 172 543 0.0 6 540 2 0 0 1 173 647 0.0 6 643 3 1 174 605 0.0 6 594 10 0 0 1 175 816 0.0 6 810 5 0 0 0 0 1 176 602 0.0 6 592 8 0 0 1 1 177 580 0.0 6 570 9 0 1 178 539 0.0 6 538 1 179 620 0.0 6 612 7 1 180 419 0.0 6 414 4 1 181 570 0.0 6 559 9 1 0 1 182 383 0.0 6 374 9 183 495 0.0 6 485 9 0 0 1 184 401 0.0 6 396 4 1 185 403 0.0 6 395 8 186 747 0.0 6 735 10 1 0 1 187 830 0.0 6 812 17 0 0 1 188 1025 0.0 6 1017 6 0 1 0 1 189 597 0.0 6 584 13 190 900 0.0 6 881 17 0 1 0 0 1 191 660 0.0 6 642 17 0 0 1 192 635 0.0 6 624 10 1 193 401 0.0 6 391 10 194 622 0.0 6 611 9 1 1 195 491 0.0 6 475 14 1 0 1 196 1031 0.0 6 1011 20 197 356 0.0 6 345 10 1 198 545 0.0 6 533 12 199 1170 0.0 6 1143 27 200 430 0.0 6 422 7 1 201 607 0.0 6 593 12 1 0 0 1 202 410 0.0 6 395 15 203 281 0.0 6 274 7 204 333 0.0 6 326 6 1 205 213 0.0 6 211 2 206 220 0.0 6 218 2 207 152 0.0 6 150 0 1 0 0 1 208 202 0.0 6 200 2 209 234 0.0 6 228 5 0 1 210 201 0.0 6 196 5 211 275 0.0 6 267 8 212 257 0.0 6 256 1 213 70 0.0 6 68 2 214 120 0.0 6 119 1 215 132 0.0 6 130 2 216 190 0.0 6 184 5 0 1 217 393 0.0 6 386 6 1 218 190 0.0 6 183 5 1 0 1 219 252 0.0 6 240 12 220 148 0.0 6 146 2 221 267 0.0 6 259 7 0 0 0 0 1 222 123 0.0 6 121 2 223 152 0.0 6 147 5 224 165 0.0 6 163 2 225 265 0.0 6 257 8 226 230 0.0 6 226 4 227 147 0.0 6 145 2 228 90 0.0 6 88 2 229 182 0.0 6 180 2 230 68 0.0 6 66 2 231 112 0.0 6 107 5 232 110 0.0 6 108 2 233 121 0.0 6 121 234 70 0.0 6 69 1 235 71 0.0 6 69 2 236 15 0.0 6 14 1 237 35 0.0 6 33 2 238 14 0.0 6 14 239 16 0.0 6 16 240 35 0.0 6 33 1 1 241 42 0.0 6 41 1 242 73 0.0 6 70 3 243 80 0.0 6 78 2 244 93 0.0 6 93 245 17 0.0 6 17 246 25 0.0 6 24 1 247 13 0.0 6 13 248 16 0.0 6 16 249 19 0.0 6 19 250 12 0.0 6 12 251 22 0.0 6 20 2 252 13 0.0 6 12 1 253 6 0.0 6 6 254 20 0.0 6 20 256 6 0.0 6 6 259 1 0.0 6 1 261 1 0.0 6 1 263 1 0.0 6 1 280 2 0.0 6 2 281 6 0.0 6 6 282 21 0.0 6 21 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 969 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 68 21 0.0 6 21 69 2 0.0 6 1 1 70 5 0.0 6 5 71 7 0.0 6 7 72 13 0.0 6 13 73 10 0.0 6 10 74 12 0.0 6 12 75 8 0.0 6 8 76 7 0.0 6 7 77 8 0.0 6 7 0 0 1 78 17 0.0 6 17 79 9 0.0 6 9 80 6 0.0 6 6 81 14 0.0 6 14 82 10 0.0 6 10 83 31 0.0 6 31 84 13 0.0 6 12 0 0 0 1 85 18 0.0 6 18 86 8 0.0 6 8 87 6 0.0 6 5 0 0 0 1 88 10 0.0 6 10 89 13 0.0 6 13 90 3 0.0 6 3 91 3 0.0 6 3 92 13 0.0 6 13 93 9 0.0 6 9 94 12 0.0 6 12 95 2 0.0 6 2 96 4 0.0 6 4 97 15 0.0 6 15 98 15 0.0 6 15 99 21 0.0 6 21 100 5 0.0 6 5 101 7 0.0 6 7 102 9 0.0 6 9 103 7 0.0 6 7 104 9 0.0 6 9 105 1 0.0 6 1 106 2 0.0 6 2 107 9 0.0 6 9 108 4 0.0 6 4 109 4 0.0 6 4 110 6 0.0 6 6 111 5 0.0 6 4 0 0 0 1 112 8 0.0 6 8 113 7 0.0 6 7 114 9 0.0 6 9 115 15 0.0 6 15 116 7 0.0 6 7 117 11 0.0 6 11 118 13 0.0 6 12 0 0 0 1 119 34 0.0 6 34 120 4 0.0 6 4 121 15 0.0 6 15 122 6 0.0 6 6 123 8 0.0 6 7 0 0 0 1 124 2 0.0 6 2 125 6 0.0 6 6 126 3 0.0 6 3 129 3 0.0 6 3 130 9 0.0 6 9 131 34 0.0 6 34 132 3 0.0 6 2 0 0 0 1 133 3 0.0 6 3 134 2 0.0 6 2 135 2 0.0 6 2 136 6 0.0 6 6 137 4 0.0 6 4 138 5 0.0 6 4 0 0 0 1 139 6 0.0 6 6 140 30 0.0 6 30 141 1 0.0 6 1 142 2 0.0 6 2 143 2 0.0 6 2 144 1 0.0 6 0 0 0 0 1 147 7 0.0 6 7 148 17 0.0 6 17 149 5 0.0 6 5 150 5 0.0 6 5 151 8 0.0 6 8 152 4 0.0 6 3 0 0 0 1 153 6 0.0 6 6 154 10 0.0 6 10 155 44 0.0 6 44 156 9 0.0 6 9 157 23 0.0 6 23 158 10 0.0 6 10 159 1 0.0 6 1 161 3 0.0 6 3 162 1 0.0 6 1 163 1 0.0 6 1 164 5 0.0 6 5 168 14 0.0 6 14 169 8 0.0 6 8 170 22 0.0 6 22 171 8 0.0 6 8 172 4 0.0 6 4 173 1 0.0 6 1 174 1 0.0 6 1 175 5 0.0 6 5 176 1 0.0 6 1 177 3 0.0 6 3 178 9 0.0 6 9 179 2 0.0 6 2 180 1 0.0 6 1 181 1 0.0 6 1 182 7 0.0 6 7 183 5 0.0 6 5 185 1 0.0 6 1 186 3 0.0 6 3 187 5 0.0 6 5 188 1 0.0 6 1 189 2 0.0 6 2 192 1 0.0 6 1 194 1 0.0 6 1 196 5 0.0 6 5 197 3 0.0 6 3 198 4 0.0 6 4 201 2 0.0 6 2 202 2 0.0 6 2 204 1 0.0 6 1 205 4 0.0 6 4 207 1 0.0 6 1 212 1 0.0 6 1 215 1 0.0 6 0 1 219 1 0.0 6 1 220 1 0.0 6 1 221 3 0.0 6 3 235 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_12_183_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_12_410_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_12_183_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_12_410_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.15 s (75 µs/read; 0.80 M reads/minute). === Summary === Total read pairs processed: 41,988 Read 1 with adapter: 41,986 (100.0%) Read 2 with adapter: 41,962 (99.9%) == Read fate breakdown == Pairs that were too short: 20 (0.0%) Pairs discarded as untrimmed: 28 (0.1%) Pairs written (passing filters): 41,940 (99.9%) Total basepairs processed: 18,543,277 bp Read 1: 9,992,384 bp Read 2: 8,550,893 bp Total written (filtered): 2,771,423 bp (14.9%) Read 1: 1,417,906 bp Read 2: 1,353,517 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 40536 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 656.1 0 0 1 4 1 164.0 1 0 1 6 2 10.3 1 1 0 1 9 2 0.2 2 2 11 1 0.0 3 1 13 1 0.0 3 1 17 2 0.0 5 1 0 1 18 1 0.0 5 0 1 19 2 0.0 5 1 1 20 43 0.0 6 42 0 0 1 21 25 0.0 6 24 1 22 14 0.0 6 13 1 23 19 0.0 6 18 1 24 8 0.0 6 7 0 1 25 15 0.0 6 14 1 26 10 0.0 6 10 27 6 0.0 6 6 28 4 0.0 6 4 29 6 0.0 6 6 30 2 0.0 6 2 31 2 0.0 6 2 32 4 0.0 6 3 1 33 3 0.0 6 3 34 4 0.0 6 4 35 6 0.0 6 6 36 3 0.0 6 3 37 3 0.0 6 3 38 3 0.0 6 2 0 0 1 39 4 0.0 6 3 1 40 1 0.0 6 1 41 2 0.0 6 2 42 3 0.0 6 2 1 44 3 0.0 6 3 46 3 0.0 6 3 49 2 0.0 6 2 50 4 0.0 6 3 1 51 2 0.0 6 2 52 2 0.0 6 2 53 1 0.0 6 1 54 5 0.0 6 5 55 2 0.0 6 2 57 4 0.0 6 3 1 58 3 0.0 6 2 0 0 0 0 1 60 2 0.0 6 2 61 1 0.0 6 1 62 5 0.0 6 5 63 2 0.0 6 2 64 2 0.0 6 2 65 3 0.0 6 2 1 66 3 0.0 6 3 67 2 0.0 6 2 68 2 0.0 6 2 69 4 0.0 6 2 2 70 4 0.0 6 4 71 4 0.0 6 4 72 3 0.0 6 3 73 4 0.0 6 4 74 2 0.0 6 1 1 75 4 0.0 6 4 76 3 0.0 6 3 77 3 0.0 6 3 78 2 0.0 6 2 79 11 0.0 6 11 80 4 0.0 6 4 81 1 0.0 6 1 82 3 0.0 6 3 83 6 0.0 6 5 0 0 0 0 0 1 84 2 0.0 6 2 85 1 0.0 6 1 86 7 0.0 6 7 87 10 0.0 6 10 88 6 0.0 6 6 89 1 0.0 6 1 90 1 0.0 6 1 91 9 0.0 6 9 92 5 0.0 6 5 93 1 0.0 6 1 94 7 0.0 6 7 95 8 0.0 6 7 1 96 7 0.0 6 7 97 1 0.0 6 1 98 5 0.0 6 5 99 3 0.0 6 3 100 7 0.0 6 7 101 6 0.0 6 5 1 102 10 0.0 6 10 103 12 0.0 6 12 104 12 0.0 6 11 0 1 105 11 0.0 6 11 106 15 0.0 6 15 107 10 0.0 6 9 1 108 14 0.0 6 14 109 22 0.0 6 22 110 19 0.0 6 18 1 111 16 0.0 6 16 112 9 0.0 6 8 1 113 5 0.0 6 5 114 14 0.0 6 14 115 9 0.0 6 9 116 10 0.0 6 10 117 10 0.0 6 10 118 11 0.0 6 11 119 7 0.0 6 7 120 10 0.0 6 10 121 7 0.0 6 7 122 9 0.0 6 9 123 4 0.0 6 3 1 124 3 0.0 6 3 125 13 0.0 6 13 126 19 0.0 6 17 1 0 0 1 127 21 0.0 6 21 128 11 0.0 6 11 129 19 0.0 6 19 130 7 0.0 6 6 1 131 13 0.0 6 13 132 6 0.0 6 6 133 21 0.0 6 20 1 134 21 0.0 6 20 1 135 23 0.0 6 23 136 13 0.0 6 13 137 31 0.0 6 31 138 28 0.0 6 28 139 38 0.0 6 38 140 48 0.0 6 48 141 81 0.0 6 80 0 0 0 0 1 142 501 0.0 6 499 2 143 1330 0.0 6 1326 3 0 0 1 144 953 0.0 6 952 1 145 250 0.0 6 249 1 146 127 0.0 6 124 3 147 728 0.0 6 727 1 148 897 0.0 6 896 1 149 111 0.0 6 111 150 41 0.0 6 41 151 22 0.0 6 22 152 24 0.0 6 24 153 22 0.0 6 22 154 25 0.0 6 24 1 155 33 0.0 6 33 156 32 0.0 6 31 1 157 51 0.0 6 50 1 158 58 0.0 6 56 2 159 107 0.0 6 107 160 846 0.0 6 845 1 161 107 0.0 6 107 162 31 0.0 6 31 163 43 0.0 6 43 164 35 0.0 6 35 165 54 0.0 6 53 1 166 35 0.0 6 35 167 43 0.0 6 43 168 37 0.0 6 37 169 57 0.0 6 55 1 0 0 0 0 1 170 38 0.0 6 38 171 93 0.0 6 92 1 172 531 0.0 6 531 173 79 0.0 6 79 174 36 0.0 6 36 175 31 0.0 6 31 176 27 0.0 6 26 1 177 50 0.0 6 48 2 178 53 0.0 6 53 179 45 0.0 6 45 180 39 0.0 6 39 181 43 0.0 6 43 182 60 0.0 6 60 183 147 0.0 6 144 3 184 85 0.0 6 85 185 63 0.0 6 63 186 137 0.0 6 137 187 136 0.0 6 136 188 128 0.0 6 128 189 335 0.0 6 333 1 0 1 190 1693 0.0 6 1692 1 191 226 0.0 6 224 2 192 139 0.0 6 139 193 128 0.0 6 127 1 194 288 0.0 6 287 1 195 619 0.0 6 617 2 196 259 0.0 6 257 1 1 197 427 0.0 6 425 2 198 1667 0.0 6 1667 199 214 0.0 6 213 1 200 150 0.0 6 149 1 201 152 0.0 6 151 1 202 149 0.0 6 148 1 203 311 0.0 6 311 204 220 0.0 6 220 205 277 0.0 6 277 206 394 0.0 6 391 2 0 0 0 0 1 207 312 0.0 6 312 208 283 0.0 6 281 2 209 258 0.0 6 256 2 210 376 0.0 6 375 1 211 1048 0.0 6 1046 2 212 391 0.0 6 390 1 213 354 0.0 6 354 214 430 0.0 6 428 2 215 709 0.0 6 708 1 216 1754 0.0 6 1751 3 217 367 0.0 6 367 218 418 0.0 6 417 1 219 520 0.0 6 520 220 333 0.0 6 331 2 221 455 0.0 6 452 3 222 498 0.0 6 496 2 223 741 0.0 6 739 2 224 670 0.0 6 669 1 225 753 0.0 6 752 1 226 917 0.0 6 912 5 227 1068 0.0 6 1066 2 228 1965 0.0 6 1960 5 229 192 0.0 6 191 1 230 138 0.0 6 137 1 231 95 0.0 6 93 2 232 111 0.0 6 111 233 127 0.0 6 126 1 234 84 0.0 6 83 1 235 80 0.0 6 80 236 72 0.0 6 69 2 0 0 1 237 69 0.0 6 69 238 68 0.0 6 68 239 124 0.0 6 124 240 82 0.0 6 81 1 241 158 0.0 6 158 242 134 0.0 6 132 2 243 159 0.0 6 159 244 348 0.0 6 348 245 130 0.0 6 129 1 246 157 0.0 6 156 1 247 223 0.0 6 222 1 248 346 0.0 6 345 1 249 368 0.0 6 366 2 250 474 0.0 6 473 1 251 264 0.0 6 263 1 252 208 0.0 6 206 2 253 279 0.0 6 278 1 254 311 0.0 6 309 2 255 476 0.0 6 476 256 966 0.0 6 966 257 119 0.0 6 117 2 258 135 0.0 6 133 2 259 285 0.0 6 282 3 260 50 0.0 6 50 261 27 0.0 6 26 1 262 31 0.0 6 30 1 263 63 0.0 6 62 1 264 34 0.0 6 33 1 265 37 0.0 6 36 1 266 29 0.0 6 29 267 54 0.0 6 52 2 268 80 0.0 6 79 1 269 75 0.0 6 75 270 26 0.0 6 25 1 271 52 0.0 6 52 272 66 0.0 6 64 2 273 72 0.0 6 71 1 274 53 0.0 6 52 1 275 74 0.0 6 71 3 276 155 0.0 6 153 2 277 221 0.0 6 219 2 278 517 0.0 6 512 5 279 268 0.0 6 263 5 280 9 0.0 6 7 2 281 5 0.0 6 5 282 2 0.0 6 2 283 1 0.0 6 1 289 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1450 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 1 0.0 6 1 70 1 0.0 6 1 71 2 0.0 6 2 72 42 0.0 6 42 73 53 0.0 6 53 74 28 0.0 6 27 1 75 8 0.0 6 8 76 8 0.0 6 8 77 41 0.0 6 41 78 30 0.0 6 30 79 1 0.0 6 1 82 1 0.0 6 1 89 15 0.0 6 15 90 40 0.0 6 40 91 1 0.0 6 1 101 7 0.0 6 7 102 24 0.0 6 24 103 1 0.0 6 1 112 1 0.0 6 1 114 1 0.0 6 1 115 1 0.0 6 0 1 117 2 0.0 6 2 118 2 0.0 6 2 119 19 0.0 6 19 120 62 0.0 6 62 121 4 0.0 6 4 124 2 0.0 6 2 125 2 0.0 6 2 127 16 0.0 6 16 128 51 0.0 6 51 129 1 0.0 6 1 130 2 0.0 6 2 131 5 0.0 6 5 132 2 0.0 6 2 133 8 0.0 6 8 134 1 0.0 6 1 135 2 0.0 6 2 139 1 0.0 6 1 140 14 0.0 6 14 141 52 0.0 6 52 142 2 0.0 6 2 143 3 0.0 6 3 144 3 0.0 6 3 145 28 0.0 6 28 146 95 0.0 6 95 147 6 0.0 6 6 150 1 0.0 6 1 152 3 0.0 6 3 153 15 0.0 6 15 154 2 0.0 6 2 156 4 0.0 6 4 157 84 0.0 6 84 158 214 0.0 6 214 159 10 0.0 6 10 162 1 0.0 6 1 168 8 0.0 6 8 169 23 0.0 6 23 170 2 0.0 6 2 174 1 0.0 6 1 177 1 0.0 6 1 180 1 0.0 6 1 181 1 0.0 6 1 182 12 0.0 6 12 183 2 0.0 6 2 217 6 0.0 6 5 1 218 65 0.0 6 65 219 184 0.0 6 184 220 2 0.0 6 2 221 26 0.0 6 26 222 68 0.0 6 68 223 5 0.0 6 5 229 1 0.0 6 1 231 2 0.0 6 2 232 8 0.0 6 8 233 1 0.0 6 1 248 1 0.0 6 1 249 4 0.0 6 4 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 40440 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 656.1 0 0 1 4 1 164.0 1 1 11 1 0.0 3 1 12 1 0.0 3 0 1 13 7 0.0 3 3 4 14 5 0.0 4 5 15 2 0.0 4 1 1 16 2 0.0 4 2 17 9 0.0 5 9 18 8 0.0 5 8 19 6 0.0 5 6 20 22 0.0 6 21 1 21 13 0.0 6 13 22 9 0.0 6 8 1 23 17 0.0 6 14 2 0 1 24 10 0.0 6 9 1 25 8 0.0 6 7 1 26 9 0.0 6 7 2 27 7 0.0 6 6 1 28 5 0.0 6 5 29 4 0.0 6 3 1 30 4 0.0 6 4 31 6 0.0 6 5 1 32 1 0.0 6 1 33 6 0.0 6 6 34 3 0.0 6 2 1 35 6 0.0 6 5 0 0 0 0 0 1 36 8 0.0 6 8 37 3 0.0 6 3 38 4 0.0 6 3 1 39 5 0.0 6 5 40 9 0.0 6 7 2 41 5 0.0 6 4 1 42 7 0.0 6 5 2 43 7 0.0 6 6 1 44 7 0.0 6 6 1 45 4 0.0 6 4 46 10 0.0 6 10 47 2 0.0 6 2 48 5 0.0 6 5 49 7 0.0 6 5 2 50 6 0.0 6 6 51 5 0.0 6 5 52 8 0.0 6 8 53 3 0.0 6 3 54 6 0.0 6 6 55 5 0.0 6 3 1 1 56 4 0.0 6 4 57 4 0.0 6 4 58 6 0.0 6 6 59 9 0.0 6 9 60 2 0.0 6 1 1 61 5 0.0 6 5 62 4 0.0 6 3 1 63 8 0.0 6 7 1 64 9 0.0 6 7 1 0 0 0 0 1 65 8 0.0 6 8 66 13 0.0 6 13 67 23 0.0 6 21 2 68 68 0.0 6 62 6 69 22 0.0 6 20 2 70 34 0.0 6 33 1 71 9 0.0 6 9 72 16 0.0 6 15 1 73 17 0.0 6 15 0 1 0 0 1 74 13 0.0 6 12 1 75 22 0.0 6 20 2 76 6 0.0 6 6 77 7 0.0 6 7 78 3 0.0 6 3 79 12 0.0 6 12 80 11 0.0 6 11 81 12 0.0 6 11 1 82 21 0.0 6 21 83 19 0.0 6 18 1 84 25 0.0 6 24 1 85 13 0.0 6 13 86 14 0.0 6 11 2 1 87 21 0.0 6 19 2 88 14 0.0 6 12 2 89 13 0.0 6 13 90 19 0.0 6 17 2 91 29 0.0 6 27 1 1 92 18 0.0 6 17 1 93 18 0.0 6 17 1 94 25 0.0 6 24 1 95 24 0.0 6 23 1 96 22 0.0 6 21 1 97 21 0.0 6 20 1 98 9 0.0 6 9 99 16 0.0 6 15 1 100 17 0.0 6 16 1 101 28 0.0 6 26 2 102 22 0.0 6 19 2 0 0 0 0 1 103 33 0.0 6 31 1 0 0 0 0 1 104 31 0.0 6 30 0 1 105 46 0.0 6 43 3 106 20 0.0 6 19 1 107 32 0.0 6 32 108 33 0.0 6 30 3 109 28 0.0 6 28 110 31 0.0 6 27 4 111 39 0.0 6 38 1 112 49 0.0 6 48 1 113 51 0.0 6 51 114 49 0.0 6 45 4 115 36 0.0 6 36 116 68 0.0 6 67 1 117 36 0.0 6 35 1 118 54 0.0 6 51 2 1 119 73 0.0 6 70 3 120 47 0.0 6 45 2 121 69 0.0 6 67 1 0 1 122 51 0.0 6 46 5 123 55 0.0 6 52 3 124 58 0.0 6 55 3 125 73 0.0 6 71 2 126 95 0.0 6 93 2 127 60 0.0 6 60 128 79 0.0 6 76 3 129 93 0.0 6 91 1 0 1 130 101 0.0 6 101 131 91 0.0 6 89 1 0 0 1 132 131 0.0 6 128 3 133 103 0.0 6 102 1 134 119 0.0 6 119 135 199 0.0 6 194 4 0 1 136 182 0.0 6 179 3 137 160 0.0 6 158 2 138 179 0.0 6 177 2 139 257 0.0 6 254 3 140 549 0.0 6 547 2 141 1151 0.0 6 1146 5 142 829 0.0 6 824 4 1 143 434 0.0 6 430 4 144 267 0.0 6 265 2 145 758 0.0 6 753 4 1 146 733 0.0 6 728 5 147 221 0.0 6 219 1 0 1 148 139 0.0 6 134 5 149 182 0.0 6 180 2 150 317 0.0 6 309 8 151 369 0.0 6 365 4 152 286 0.0 6 276 8 1 0 0 0 1 153 414 0.0 6 401 11 2 154 382 0.0 6 378 4 155 345 0.0 6 341 3 1 156 378 0.0 6 373 5 157 368 0.0 6 359 5 3 0 0 1 158 655 0.0 6 650 5 159 262 0.0 6 260 2 160 300 0.0 6 298 2 161 313 0.0 6 313 162 258 0.0 6 258 163 269 0.0 6 265 4 164 349 0.0 6 345 4 165 324 0.0 6 322 2 166 370 0.0 6 367 3 167 417 0.0 6 414 3 168 377 0.0 6 369 8 169 486 0.0 6 479 6 1 170 584 0.0 6 580 4 171 521 0.0 6 516 5 172 714 0.0 6 704 9 1 173 404 0.0 6 396 7 0 0 0 0 1 174 432 0.0 6 425 7 175 509 0.0 6 507 2 176 549 0.0 6 539 9 1 177 481 0.0 6 477 4 178 323 0.0 6 313 10 179 468 0.0 6 466 2 180 403 0.0 6 390 12 1 181 424 0.0 6 415 8 0 0 1 182 679 0.0 6 669 9 1 183 509 0.0 6 499 10 184 669 0.0 6 657 11 0 0 1 185 890 0.0 6 871 14 4 1 186 1067 0.0 6 1049 16 2 187 800 0.0 6 795 5 188 953 0.0 6 951 2 189 461 0.0 6 454 7 190 480 0.0 6 477 3 191 449 0.0 6 449 192 619 0.0 6 616 3 193 513 0.0 6 513 194 513 0.0 6 511 2 195 587 0.0 6 586 1 196 624 0.0 6 623 1 197 583 0.0 6 581 2 198 356 0.0 6 354 2 199 416 0.0 6 413 3 200 325 0.0 6 324 1 201 327 0.0 6 325 2 202 277 0.0 6 274 3 203 295 0.0 6 291 3 0 0 1 204 404 0.0 6 403 1 205 229 0.0 6 228 0 1 206 326 0.0 6 325 1 207 359 0.0 6 359 208 298 0.0 6 297 1 209 334 0.0 6 332 2 210 134 0.0 6 134 211 182 0.0 6 182 212 261 0.0 6 259 2 213 156 0.0 6 156 214 566 0.0 6 563 1 1 1 215 183 0.0 6 183 216 173 0.0 6 173 217 182 0.0 6 182 218 307 0.0 6 307 219 139 0.0 6 138 1 220 171 0.0 6 169 1 0 0 1 221 55 0.0 6 54 1 222 231 0.0 6 229 2 223 92 0.0 6 91 1 224 89 0.0 6 89 225 150 0.0 6 149 0 1 226 147 0.0 6 146 1 227 71 0.0 6 70 1 228 81 0.0 6 81 229 67 0.0 6 67 230 22 0.0 6 22 231 36 0.0 6 36 232 41 0.0 6 41 233 30 0.0 6 30 234 27 0.0 6 27 235 22 0.0 6 22 236 50 0.0 6 50 237 14 0.0 6 14 238 4 0.0 6 4 239 4 0.0 6 3 0 0 0 0 0 1 240 5 0.0 6 5 241 2 0.0 6 2 242 8 0.0 6 6 2 243 4 0.0 6 4 244 1 0.0 6 1 245 3 0.0 6 3 247 2 0.0 6 2 248 1 0.0 6 1 250 1 0.0 6 1 251 1 0.0 6 1 252 2 0.0 6 2 278 1 0.0 6 1 279 4 0.0 6 4 280 104 0.0 6 103 1 281 3 0.0 6 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1522 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 4 0.0 6 4 71 28 0.0 6 28 72 81 0.0 6 81 73 47 0.0 6 47 74 10 0.0 6 10 75 13 0.0 6 12 0 0 0 1 76 42 0.0 6 42 77 29 0.0 6 29 78 2 0.0 6 2 81 1 0.0 6 1 84 1 0.0 6 1 87 4 0.0 6 4 88 6 0.0 6 6 89 48 0.0 6 48 90 1 0.0 6 1 93 1 0.0 6 0 0 0 0 1 99 1 0.0 6 1 100 1 0.0 6 1 101 31 0.0 6 31 112 2 0.0 6 2 113 1 0.0 6 1 116 1 0.0 6 1 117 1 0.0 6 1 118 9 0.0 6 9 119 83 0.0 6 83 124 1 0.0 6 1 125 3 0.0 6 3 126 23 0.0 6 23 127 90 0.0 6 90 128 3 0.0 6 3 129 1 0.0 6 1 130 3 0.0 6 3 131 2 0.0 6 2 132 15 0.0 6 15 134 2 0.0 6 2 139 13 0.0 6 12 1 140 55 0.0 6 55 141 4 0.0 6 4 142 4 0.0 6 4 143 2 0.0 6 2 144 15 0.0 6 15 145 124 0.0 6 124 146 4 0.0 6 4 148 2 0.0 6 0 0 0 2 149 1 0.0 6 0 0 0 0 1 150 2 0.0 6 2 151 8 0.0 6 8 152 21 0.0 6 21 153 5 0.0 6 4 0 0 1 154 1 0.0 6 1 155 5 0.0 6 4 1 156 71 0.0 6 70 0 0 0 1 157 294 0.0 6 292 2 158 9 0.0 6 8 1 160 1 0.0 6 1 161 2 0.0 6 2 165 1 0.0 6 1 166 2 0.0 6 2 167 13 0.0 6 13 168 28 0.0 6 28 169 1 0.0 6 1 172 1 0.0 6 1 173 1 0.0 6 1 176 1 0.0 6 1 180 2 0.0 6 2 181 13 0.0 6 13 212 2 0.0 6 2 215 1 0.0 6 1 216 3 0.0 6 2 1 217 42 0.0 6 42 218 158 0.0 6 157 1 219 5 0.0 6 5 220 1 0.0 6 1 221 9 0.0 6 9 222 2 0.0 6 0 1 0 0 1 231 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_13_184_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_13_411_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_13_184_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_13_411_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.12 s (59 µs/read; 1.02 M reads/minute). === Summary === Total read pairs processed: 70,283 Read 1 with adapter: 70,280 (100.0%) Read 2 with adapter: 70,228 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 58 (0.1%) Pairs written (passing filters): 70,225 (99.9%) Total basepairs processed: 34,213,422 bp Read 1: 19,467,960 bp Read 2: 14,745,462 bp Total written (filtered): 4,363,555 bp (12.8%) Read 1: 2,252,110 bp Read 2: 2,111,445 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 70190 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1098.2 0 0 1 8 1 1.1 2 0 1 15 3 0.0 4 3 17 1 0.0 5 1 19 1 0.0 5 1 20 13 0.0 6 13 21 12 0.0 6 12 22 8 0.0 6 8 23 3 0.0 6 3 24 2 0.0 6 1 0 1 25 6 0.0 6 6 26 8 0.0 6 6 2 27 5 0.0 6 5 28 6 0.0 6 4 2 29 2 0.0 6 2 30 4 0.0 6 4 31 2 0.0 6 2 33 2 0.0 6 2 34 3 0.0 6 3 36 3 0.0 6 2 1 37 5 0.0 6 5 38 4 0.0 6 4 39 1 0.0 6 1 40 2 0.0 6 2 41 1 0.0 6 0 0 1 42 9 0.0 6 7 1 0 0 0 0 1 43 3 0.0 6 2 0 0 1 44 6 0.0 6 5 1 45 4 0.0 6 3 1 46 4 0.0 6 3 0 1 47 1 0.0 6 1 49 3 0.0 6 1 1 1 50 2 0.0 6 2 53 4 0.0 6 4 54 5 0.0 6 5 55 2 0.0 6 2 56 10 0.0 6 10 57 6 0.0 6 4 0 1 0 0 1 58 4 0.0 6 3 1 59 2 0.0 6 2 61 1 0.0 6 1 62 3 0.0 6 3 63 5 0.0 6 5 64 7 0.0 6 7 65 3 0.0 6 3 67 2 0.0 6 2 68 3 0.0 6 3 69 4 0.0 6 4 70 1 0.0 6 0 1 72 1 0.0 6 1 73 2 0.0 6 1 1 74 2 0.0 6 2 75 1 0.0 6 1 76 1 0.0 6 1 77 6 0.0 6 5 1 78 11 0.0 6 9 1 1 79 2 0.0 6 2 80 1 0.0 6 1 81 2 0.0 6 2 82 2 0.0 6 2 85 1 0.0 6 1 86 9 0.0 6 7 1 0 1 87 5 0.0 6 4 0 1 88 1 0.0 6 1 89 7 0.0 6 7 90 3 0.0 6 3 91 3 0.0 6 3 92 5 0.0 6 5 93 7 0.0 6 6 1 94 2 0.0 6 2 96 1 0.0 6 1 97 9 0.0 6 9 98 7 0.0 6 6 1 99 3 0.0 6 3 100 11 0.0 6 10 1 101 18 0.0 6 16 1 0 0 1 102 5 0.0 6 4 1 103 6 0.0 6 6 104 6 0.0 6 6 105 7 0.0 6 6 1 106 2 0.0 6 2 107 3 0.0 6 2 1 108 4 0.0 6 4 109 2 0.0 6 2 111 5 0.0 6 5 112 8 0.0 6 8 113 11 0.0 6 11 114 6 0.0 6 6 115 16 0.0 6 16 116 7 0.0 6 6 1 117 1 0.0 6 0 1 118 4 0.0 6 3 0 0 0 0 0 1 119 16 0.0 6 14 2 120 10 0.0 6 9 1 121 4 0.0 6 4 122 12 0.0 6 12 123 12 0.0 6 12 124 10 0.0 6 8 2 125 17 0.0 6 14 3 126 7 0.0 6 7 127 8 0.0 6 8 128 18 0.0 6 18 129 9 0.0 6 9 130 6 0.0 6 5 1 131 5 0.0 6 5 132 5 0.0 6 5 133 14 0.0 6 14 134 12 0.0 6 10 1 1 135 27 0.0 6 27 136 25 0.0 6 25 137 52 0.0 6 48 4 138 48 0.0 6 44 3 1 139 15 0.0 6 14 1 140 53 0.0 6 51 2 141 55 0.0 6 54 0 1 142 49 0.0 6 48 1 143 114 0.0 6 113 1 144 193 0.0 6 193 145 93 0.0 6 92 1 146 77 0.0 6 76 1 147 124 0.0 6 120 4 148 105 0.0 6 101 4 149 83 0.0 6 78 4 1 150 103 0.0 6 101 2 151 104 0.0 6 99 5 152 107 0.0 6 107 153 44 0.0 6 44 154 55 0.0 6 55 155 47 0.0 6 47 156 60 0.0 6 59 1 157 107 0.0 6 105 2 158 54 0.0 6 50 4 159 117 0.0 6 115 2 160 110 0.0 6 108 2 161 87 0.0 6 86 1 162 83 0.0 6 82 1 163 69 0.0 6 67 2 164 56 0.0 6 51 5 165 66 0.0 6 64 2 166 49 0.0 6 49 167 52 0.0 6 51 1 168 40 0.0 6 38 2 169 58 0.0 6 58 170 49 0.0 6 47 2 171 54 0.0 6 51 3 172 70 0.0 6 67 3 173 83 0.0 6 79 3 1 174 119 0.0 6 115 2 2 175 110 0.0 6 107 3 176 110 0.0 6 108 2 177 99 0.0 6 97 2 178 92 0.0 6 89 3 179 201 0.0 6 197 3 1 180 126 0.0 6 124 2 181 90 0.0 6 88 2 182 172 0.0 6 170 1 1 183 179 0.0 6 177 2 184 219 0.0 6 211 7 0 1 185 202 0.0 6 195 7 186 243 0.0 6 232 10 1 187 109 0.0 6 105 4 188 103 0.0 6 99 4 189 125 0.0 6 119 6 190 194 0.0 6 190 4 191 210 0.0 6 204 6 192 178 0.0 6 174 4 193 229 0.0 6 219 10 194 211 0.0 6 197 13 1 195 271 0.0 6 264 7 196 349 0.0 6 339 10 197 271 0.0 6 262 9 198 364 0.0 6 352 12 199 286 0.0 6 274 12 200 217 0.0 6 216 1 201 213 0.0 6 210 3 202 275 0.0 6 264 11 203 333 0.0 6 327 6 204 239 0.0 6 233 4 1 1 205 321 0.0 6 312 9 206 344 0.0 6 336 8 207 386 0.0 6 373 13 208 474 0.0 6 464 9 1 209 393 0.0 6 386 7 210 248 0.0 6 240 8 211 231 0.0 6 226 4 0 1 212 232 0.0 6 225 7 213 303 0.0 6 295 8 214 294 0.0 6 285 9 215 254 0.0 6 249 4 1 216 263 0.0 6 257 6 217 422 0.0 6 410 12 218 354 0.0 6 350 4 219 338 0.0 6 331 7 220 257 0.0 6 251 6 221 272 0.0 6 265 7 222 562 0.0 6 554 8 223 190 0.0 6 187 2 1 224 157 0.0 6 156 1 225 407 0.0 6 400 7 226 1024 0.0 6 1002 19 3 227 764 0.0 6 744 19 1 228 778 0.0 6 765 13 229 573 0.0 6 556 16 1 230 637 0.0 6 623 14 231 420 0.0 6 403 16 1 232 626 0.0 6 612 13 1 233 668 0.0 6 648 20 234 526 0.0 6 515 10 0 0 1 235 311 0.0 6 305 6 236 295 0.0 6 289 6 237 238 0.0 6 232 6 238 358 0.0 6 347 11 239 431 0.0 6 419 11 1 240 494 0.0 6 481 13 241 813 0.0 6 794 19 242 801 0.0 6 778 22 1 243 635 0.0 6 629 6 244 478 0.0 6 461 17 245 882 0.0 6 871 11 246 277 0.0 6 273 3 1 247 408 0.0 6 401 7 248 217 0.0 6 209 7 1 249 771 0.0 6 754 17 250 480 0.0 6 466 14 251 578 0.0 6 564 13 0 0 1 252 488 0.0 6 474 12 1 1 253 385 0.0 6 372 13 254 1259 0.0 6 1231 27 1 255 693 0.0 6 679 13 0 0 1 256 461 0.0 6 449 12 257 619 0.0 6 605 13 1 258 826 0.0 6 808 17 1 259 1154 0.0 6 1136 18 260 1625 0.0 6 1593 31 1 261 577 0.0 6 565 12 262 695 0.0 6 671 24 263 2186 0.0 6 2156 27 3 264 615 0.0 6 603 12 265 1348 0.0 6 1314 34 266 449 0.0 6 434 15 267 1015 0.0 6 999 16 268 839 0.0 6 817 20 1 1 269 1020 0.0 6 997 21 1 1 270 561 0.0 6 550 9 2 271 841 0.0 6 811 30 272 1532 0.0 6 1503 29 273 1371 0.0 6 1340 31 274 1462 0.0 6 1441 19 1 1 275 1664 0.0 6 1620 41 3 276 2790 0.0 6 2732 57 1 277 2872 0.0 6 2815 57 278 2387 0.0 6 2346 37 3 0 1 279 7529 0.0 6 7191 322 16 280 159 0.0 6 137 21 1 281 28 0.0 6 23 3 1 0 1 282 1 0.0 6 1 283 3 0.0 6 3 286 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 90 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 9 0.0 6 9 74 4 0.0 6 4 75 2 0.0 6 2 76 2 0.0 6 2 78 2 0.0 6 2 79 2 0.0 6 2 80 1 0.0 6 1 81 1 0.0 6 1 82 3 0.0 6 3 83 1 0.0 6 1 85 1 0.0 6 1 86 2 0.0 6 2 87 1 0.0 6 1 89 2 0.0 6 2 90 1 0.0 6 1 91 1 0.0 6 1 92 3 0.0 6 3 93 2 0.0 6 2 100 1 0.0 6 1 102 3 0.0 6 3 107 1 0.0 6 1 109 1 0.0 6 1 110 1 0.0 6 1 111 3 0.0 6 3 112 2 0.0 6 2 113 6 0.0 6 6 114 1 0.0 6 1 115 4 0.0 6 4 128 1 0.0 6 1 137 1 0.0 6 1 139 1 0.0 6 1 147 1 0.0 6 1 152 1 0.0 6 1 153 1 0.0 6 1 154 1 0.0 6 1 158 1 0.0 6 1 161 1 0.0 6 1 162 3 0.0 6 3 164 2 0.0 6 2 168 1 0.0 6 1 171 1 0.0 6 1 180 2 0.0 6 2 181 1 0.0 6 1 197 1 0.0 6 1 201 1 0.0 6 1 202 1 0.0 6 1 203 1 0.0 6 0 0 0 0 0 0 1 209 2 0.0 6 2 221 1 0.0 6 1 238 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 70120 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 2 17.2 1 2 7 1 4.3 2 0 1 11 1 0.0 3 1 12 2 0.0 3 2 13 14 0.0 3 12 2 14 4 0.0 4 4 15 1 0.0 4 0 0 1 16 15 0.0 4 14 1 17 17 0.0 5 16 1 18 14 0.0 5 14 19 10 0.0 5 10 20 56 0.0 6 54 2 21 56 0.0 6 55 1 22 47 0.0 6 46 1 23 37 0.0 6 35 2 24 18 0.0 6 16 2 25 24 0.0 6 23 0 1 26 15 0.0 6 15 27 12 0.0 6 12 28 9 0.0 6 9 29 17 0.0 6 15 2 30 18 0.0 6 14 4 31 10 0.0 6 9 1 32 10 0.0 6 10 33 3 0.0 6 3 34 10 0.0 6 10 35 8 0.0 6 7 1 36 4 0.0 6 4 37 12 0.0 6 10 1 1 38 21 0.0 6 20 1 39 12 0.0 6 12 40 12 0.0 6 11 1 41 8 0.0 6 7 1 42 6 0.0 6 6 43 7 0.0 6 6 1 44 20 0.0 6 19 1 45 11 0.0 6 9 1 0 1 46 14 0.0 6 13 1 47 20 0.0 6 17 3 48 15 0.0 6 14 1 49 16 0.0 6 16 50 25 0.0 6 23 2 51 14 0.0 6 14 52 17 0.0 6 15 1 1 53 8 0.0 6 8 54 19 0.0 6 16 3 55 31 0.0 6 29 1 1 56 27 0.0 6 24 3 57 16 0.0 6 16 58 15 0.0 6 14 0 1 59 12 0.0 6 11 1 60 18 0.0 6 18 61 13 0.0 6 13 62 24 0.0 6 23 1 63 14 0.0 6 14 64 19 0.0 6 19 65 13 0.0 6 12 1 66 31 0.0 6 30 0 1 67 26 0.0 6 25 0 0 0 1 68 39 0.0 6 36 2 0 1 69 125 0.0 6 118 7 70 19 0.0 6 19 71 56 0.0 6 55 1 72 52 0.0 6 47 5 73 32 0.0 6 30 2 74 19 0.0 6 17 2 75 12 0.0 6 11 1 76 28 0.0 6 28 77 30 0.0 6 30 78 32 0.0 6 30 2 79 29 0.0 6 26 2 0 1 80 28 0.0 6 28 81 13 0.0 6 12 1 82 27 0.0 6 25 2 83 53 0.0 6 50 3 84 58 0.0 6 54 3 1 85 56 0.0 6 52 4 86 33 0.0 6 33 87 50 0.0 6 47 2 1 88 21 0.0 6 20 1 89 38 0.0 6 33 4 1 90 50 0.0 6 45 4 1 91 50 0.0 6 47 2 1 92 28 0.0 6 24 4 93 66 0.0 6 63 2 0 0 1 94 90 0.0 6 86 4 95 40 0.0 6 39 0 1 96 54 0.0 6 48 6 97 60 0.0 6 53 5 2 98 37 0.0 6 35 2 99 41 0.0 6 40 1 100 41 0.0 6 36 5 101 25 0.0 6 24 1 102 116 0.0 6 110 6 103 81 0.0 6 78 3 104 80 0.0 6 76 4 105 89 0.0 6 84 4 0 1 106 83 0.0 6 79 4 107 91 0.0 6 84 6 0 0 1 108 77 0.0 6 72 5 109 59 0.0 6 54 5 110 47 0.0 6 44 2 1 111 53 0.0 6 52 1 112 31 0.0 6 31 113 47 0.0 6 45 2 114 61 0.0 6 57 4 115 87 0.0 6 81 4 0 1 1 116 73 0.0 6 71 2 117 40 0.0 6 38 2 118 68 0.0 6 63 5 119 96 0.0 6 91 3 1 0 1 120 132 0.0 6 123 8 0 1 121 83 0.0 6 76 5 1 0 1 122 126 0.0 6 121 3 1 0 1 123 136 0.0 6 130 5 0 0 1 124 149 0.0 6 144 5 125 95 0.0 6 86 8 1 126 85 0.0 6 85 127 139 0.0 6 130 7 1 0 1 128 74 0.0 6 67 7 129 89 0.0 6 88 1 130 102 0.0 6 100 2 131 210 0.0 6 201 9 132 255 0.0 6 243 12 133 228 0.0 6 222 6 134 250 0.0 6 242 7 0 0 1 135 425 0.0 6 409 16 136 183 0.0 6 179 4 137 289 0.0 6 269 17 2 0 1 138 190 0.0 6 183 6 1 139 232 0.0 6 225 7 140 266 0.0 6 263 3 141 289 0.0 6 279 8 1 0 1 142 478 0.0 6 458 17 2 0 1 143 432 0.0 6 421 11 144 543 0.0 6 531 12 145 291 0.0 6 275 14 1 1 146 285 0.0 6 277 8 147 212 0.0 6 206 4 1 0 1 148 285 0.0 6 275 10 149 439 0.0 6 424 15 150 637 0.0 6 613 21 2 1 151 322 0.0 6 316 6 152 880 0.0 6 853 26 1 153 447 0.0 6 437 9 1 154 528 0.0 6 511 16 1 155 542 0.0 6 529 12 1 156 410 0.0 6 398 11 1 157 631 0.0 6 615 13 1 0 2 158 660 0.0 6 643 17 159 281 0.0 6 272 9 160 646 0.0 6 627 19 161 431 0.0 6 420 10 1 162 376 0.0 6 366 9 1 163 480 0.0 6 463 15 2 164 429 0.0 6 414 15 165 539 0.0 6 521 17 1 166 703 0.0 6 686 14 2 0 1 167 282 0.0 6 268 13 1 168 952 0.0 6 931 20 0 0 1 169 230 0.0 6 220 9 1 170 453 0.0 6 439 12 1 0 0 1 171 910 0.0 6 882 26 1 0 1 172 204 0.0 6 196 8 173 272 0.0 6 261 10 0 0 1 174 472 0.0 6 459 13 175 1069 0.0 6 1042 27 176 575 0.0 6 555 20 177 892 0.0 6 860 27 3 1 0 1 178 696 0.0 6 679 17 179 754 0.0 6 728 25 0 0 1 180 770 0.0 6 741 27 0 0 2 181 908 0.0 6 883 24 0 0 1 182 245 0.0 6 232 12 1 183 744 0.0 6 722 22 184 279 0.0 6 268 9 2 185 440 0.0 6 420 19 1 186 1684 0.0 6 1632 49 2 0 1 187 1432 0.0 6 1386 45 1 188 1444 0.0 6 1403 38 3 189 1362 0.0 6 1309 50 2 1 190 1891 0.0 6 1827 61 3 191 1511 0.0 6 1456 52 1 0 2 192 1210 0.0 6 1173 34 2 1 193 779 0.0 6 755 21 1 2 194 1220 0.0 6 1180 36 2 1 1 195 1715 0.0 6 1652 61 0 0 2 196 3113 0.0 6 3036 73 3 0 1 197 480 0.0 6 455 20 5 198 2382 0.0 6 2313 68 0 1 199 3870 0.0 6 3766 102 1 0 1 200 851 0.0 6 834 17 201 1087 0.0 6 1049 35 0 0 3 202 635 0.0 6 616 18 1 203 772 0.0 6 749 22 0 0 1 204 823 0.0 6 810 12 0 0 1 205 288 0.0 6 277 10 0 0 1 206 261 0.0 6 254 7 207 162 0.0 6 154 8 208 360 0.0 6 350 10 209 304 0.0 6 294 9 0 1 210 282 0.0 6 274 8 211 318 0.0 6 303 15 212 353 0.0 6 340 12 1 213 87 0.0 6 85 1 0 1 214 251 0.0 6 244 7 215 234 0.0 6 223 9 1 0 1 216 919 0.0 6 896 23 217 531 0.0 6 519 12 218 356 0.0 6 339 17 219 338 0.0 6 329 9 220 242 0.0 6 235 7 221 285 0.0 6 268 16 1 222 546 0.0 6 528 18 223 1390 0.0 6 1359 28 1 0 2 224 117 0.0 6 117 225 431 0.0 6 423 8 226 214 0.0 6 205 8 0 0 1 227 166 0.0 6 160 6 228 86 0.0 6 86 229 212 0.0 6 209 2 1 230 146 0.0 6 141 5 231 156 0.0 6 150 5 0 0 1 232 207 0.0 6 198 9 233 348 0.0 6 341 7 234 127 0.0 6 123 3 1 235 48 0.0 6 46 2 236 40 0.0 6 40 237 38 0.0 6 36 2 238 10 0.0 6 10 239 9 0.0 6 8 0 0 1 240 125 0.0 6 118 6 0 0 1 241 78 0.0 6 76 2 242 168 0.0 6 163 5 243 201 0.0 6 200 1 244 104 0.0 6 100 4 245 28 0.0 6 27 1 246 20 0.0 6 20 247 21 0.0 6 21 248 16 0.0 6 16 249 13 0.0 6 12 1 250 20 0.0 6 19 1 251 24 0.0 6 24 252 7 0.0 6 7 253 30 0.0 6 29 1 254 4 0.0 6 4 255 2 0.0 6 2 260 1 0.0 6 1 275 1 0.0 6 1 276 1 0.0 6 1 278 2 0.0 6 2 279 16 0.0 6 16 280 48 0.0 6 47 1 281 324 0.0 6 313 10 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 108 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 1 0.0 6 1 72 3 0.0 6 3 73 3 0.0 6 3 74 5 0.0 6 5 75 2 0.0 6 2 76 2 0.0 6 2 77 2 0.0 6 2 79 2 0.0 6 2 81 6 0.0 6 6 85 1 0.0 6 1 86 2 0.0 6 2 87 3 0.0 6 3 88 4 0.0 6 4 90 3 0.0 6 3 91 3 0.0 6 3 93 1 0.0 6 1 94 3 0.0 6 3 96 1 0.0 6 1 97 2 0.0 6 1 1 98 1 0.0 6 1 99 2 0.0 6 2 100 1 0.0 6 1 101 1 0.0 6 1 102 1 0.0 6 1 103 2 0.0 6 2 104 1 0.0 6 0 0 0 1 105 1 0.0 6 1 108 3 0.0 6 3 109 2 0.0 6 2 110 1 0.0 6 1 111 3 0.0 6 3 112 4 0.0 6 3 0 0 0 1 113 2 0.0 6 2 114 2 0.0 6 2 120 2 0.0 6 2 121 7 0.0 6 7 122 2 0.0 6 2 125 1 0.0 6 1 128 1 0.0 6 1 133 1 0.0 6 1 135 2 0.0 6 2 136 1 0.0 6 1 141 1 0.0 6 0 0 0 0 1 145 1 0.0 6 1 147 3 0.0 6 3 151 1 0.0 6 1 160 2 0.0 6 2 171 1 0.0 6 1 179 1 0.0 6 1 182 1 0.0 6 1 183 1 0.0 6 1 186 2 0.0 6 2 222 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_07_185_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_07_412_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_07_185_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_07_412_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.86 s (51 µs/read; 1.17 M reads/minute). === Summary === Total read pairs processed: 94,633 Read 1 with adapter: 94,633 (100.0%) Read 2 with adapter: 94,559 (99.9%) == Read fate breakdown == Pairs that were too short: 46 (0.0%) Pairs discarded as untrimmed: 74 (0.1%) Pairs written (passing filters): 94,513 (99.9%) Total basepairs processed: 39,786,750 bp Read 1: 22,155,681 bp Read 2: 17,631,069 bp Total written (filtered): 6,332,985 bp (15.9%) Read 1: 3,243,365 bp Read 2: 3,089,620 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 92178 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 5 369.7 1 5 5 1 92.4 1 1 6 4 23.1 1 1 0 3 7 4 5.8 2 2 0 2 8 1 1.4 2 1 10 1 0.1 3 1 13 1 0.0 3 1 14 3 0.0 4 2 1 15 2 0.0 4 1 1 16 3 0.0 4 2 1 17 6 0.0 5 6 18 1 0.0 5 1 19 7 0.0 5 7 20 118 0.0 6 115 3 21 44 0.0 6 41 3 22 52 0.0 6 50 2 23 34 0.0 6 34 24 26 0.0 6 26 25 24 0.0 6 23 1 26 12 0.0 6 11 1 27 14 0.0 6 14 28 14 0.0 6 14 29 9 0.0 6 9 30 10 0.0 6 10 31 6 0.0 6 5 1 32 7 0.0 6 7 33 7 0.0 6 7 34 13 0.0 6 11 2 35 6 0.0 6 6 36 5 0.0 6 3 2 37 9 0.0 6 8 1 38 13 0.0 6 13 39 13 0.0 6 13 40 13 0.0 6 13 41 9 0.0 6 9 42 10 0.0 6 9 1 43 5 0.0 6 5 44 9 0.0 6 9 45 7 0.0 6 5 1 0 0 1 46 7 0.0 6 6 0 1 47 6 0.0 6 6 48 10 0.0 6 9 0 0 0 0 0 1 49 5 0.0 6 4 0 1 50 10 0.0 6 9 1 51 10 0.0 6 9 1 52 9 0.0 6 8 1 53 6 0.0 6 6 54 9 0.0 6 9 55 7 0.0 6 7 56 4 0.0 6 4 57 7 0.0 6 7 58 4 0.0 6 3 1 59 3 0.0 6 3 60 3 0.0 6 3 61 6 0.0 6 6 62 3 0.0 6 3 63 13 0.0 6 13 64 9 0.0 6 9 65 7 0.0 6 7 66 5 0.0 6 5 67 6 0.0 6 6 68 7 0.0 6 7 69 17 0.0 6 17 70 9 0.0 6 9 71 12 0.0 6 12 72 10 0.0 6 10 73 9 0.0 6 8 1 74 8 0.0 6 8 75 8 0.0 6 8 76 8 0.0 6 8 77 10 0.0 6 10 78 14 0.0 6 14 79 11 0.0 6 11 80 10 0.0 6 8 2 81 13 0.0 6 12 1 82 20 0.0 6 18 2 83 13 0.0 6 13 84 10 0.0 6 10 85 12 0.0 6 12 86 17 0.0 6 16 1 87 6 0.0 6 6 88 18 0.0 6 17 1 89 8 0.0 6 8 90 9 0.0 6 8 1 91 13 0.0 6 12 0 0 1 92 8 0.0 6 8 93 16 0.0 6 15 0 0 0 1 94 14 0.0 6 14 95 8 0.0 6 7 1 96 17 0.0 6 17 97 14 0.0 6 13 1 98 15 0.0 6 14 0 1 99 19 0.0 6 19 100 17 0.0 6 17 101 29 0.0 6 28 1 102 25 0.0 6 25 103 18 0.0 6 17 1 104 22 0.0 6 22 105 29 0.0 6 28 1 106 17 0.0 6 16 1 107 13 0.0 6 12 1 108 26 0.0 6 26 109 25 0.0 6 24 1 110 24 0.0 6 24 111 22 0.0 6 22 112 30 0.0 6 28 0 1 0 0 0 1 113 31 0.0 6 30 1 114 32 0.0 6 31 0 0 0 0 0 1 115 37 0.0 6 37 116 33 0.0 6 33 117 34 0.0 6 33 1 118 35 0.0 6 33 2 119 30 0.0 6 29 1 120 39 0.0 6 39 121 31 0.0 6 30 1 122 36 0.0 6 36 123 43 0.0 6 41 2 124 31 0.0 6 31 125 36 0.0 6 35 1 126 46 0.0 6 46 127 39 0.0 6 39 128 56 0.0 6 54 2 129 63 0.0 6 63 130 57 0.0 6 56 1 131 43 0.0 6 43 132 61 0.0 6 59 2 133 72 0.0 6 72 134 40 0.0 6 40 135 49 0.0 6 49 136 50 0.0 6 50 137 70 0.0 6 70 138 64 0.0 6 64 139 101 0.0 6 99 1 0 1 140 77 0.0 6 74 2 0 1 141 121 0.0 6 121 142 128 0.0 6 128 143 271 0.0 6 270 0 1 144 547 0.0 6 544 3 145 803 0.0 6 802 1 146 600 0.0 6 597 3 147 595 0.0 6 593 2 148 724 0.0 6 722 1 0 0 0 1 149 624 0.0 6 622 1 1 150 576 0.0 6 573 3 151 706 0.0 6 705 0 1 152 685 0.0 6 683 2 153 724 0.0 6 724 154 528 0.0 6 526 2 155 470 0.0 6 468 2 156 465 0.0 6 464 1 157 534 0.0 6 532 1 0 1 158 414 0.0 6 410 4 159 456 0.0 6 455 1 160 574 0.0 6 572 2 161 530 0.0 6 529 1 162 536 0.0 6 534 2 163 569 0.0 6 568 1 164 538 0.0 6 535 3 165 502 0.0 6 501 1 166 609 0.0 6 607 2 167 500 0.0 6 500 168 516 0.0 6 516 169 607 0.0 6 601 5 0 0 0 0 1 170 502 0.0 6 502 171 565 0.0 6 562 3 172 581 0.0 6 579 2 173 513 0.0 6 512 1 174 629 0.0 6 626 3 175 653 0.0 6 649 4 176 558 0.0 6 556 2 177 482 0.0 6 479 1 0 0 0 1 1 178 608 0.0 6 607 0 0 1 179 526 0.0 6 524 2 180 614 0.0 6 614 181 1776 0.0 6 1774 2 182 951 0.0 6 949 2 183 654 0.0 6 650 3 0 1 184 718 0.0 6 716 2 185 638 0.0 6 636 2 186 647 0.0 6 646 1 187 550 0.0 6 548 1 1 188 576 0.0 6 573 3 189 743 0.0 6 741 2 190 725 0.0 6 724 1 191 676 0.0 6 673 2 0 0 0 0 1 192 848 0.0 6 840 8 193 940 0.0 6 937 2 0 0 0 1 194 990 0.0 6 987 3 195 1083 0.0 6 1080 3 196 1267 0.0 6 1264 3 197 1310 0.0 6 1302 7 1 198 1113 0.0 6 1110 3 199 1134 0.0 6 1130 4 200 940 0.0 6 938 2 201 910 0.0 6 906 4 202 1017 0.0 6 1016 1 203 958 0.0 6 948 9 0 0 1 204 1130 0.0 6 1128 2 205 1838 0.0 6 1826 12 206 1114 0.0 6 1104 8 2 207 1248 0.0 6 1244 3 0 1 208 949 0.0 6 945 3 0 0 0 0 1 209 883 0.0 6 879 3 0 1 210 1048 0.0 6 1046 2 211 1128 0.0 6 1123 4 1 212 1111 0.0 6 1109 2 213 1205 0.0 6 1200 4 1 214 1447 0.0 6 1442 2 3 215 1104 0.0 6 1093 10 1 216 1170 0.0 6 1167 2 1 217 1100 0.0 6 1097 2 1 218 1090 0.0 6 1085 4 0 0 0 1 219 807 0.0 6 806 1 220 904 0.0 6 899 4 0 0 0 0 1 221 871 0.0 6 870 0 0 0 1 222 931 0.0 6 927 4 223 960 0.0 6 957 3 224 1027 0.0 6 1025 2 225 1097 0.0 6 1094 3 226 933 0.0 6 930 3 227 875 0.0 6 872 3 228 756 0.0 6 753 2 0 0 0 0 1 229 891 0.0 6 888 3 230 746 0.0 6 741 4 1 231 729 0.0 6 723 5 1 232 745 0.0 6 743 2 233 664 0.0 6 664 234 623 0.0 6 620 2 0 0 0 1 235 635 0.0 6 633 2 236 608 0.0 6 604 4 237 559 0.0 6 555 4 238 621 0.0 6 618 1 2 239 566 0.0 6 563 3 240 537 0.0 6 535 1 1 241 598 0.0 6 595 3 242 467 0.0 6 465 2 243 394 0.0 6 390 3 0 1 244 380 0.0 6 376 4 245 353 0.0 6 350 3 246 373 0.0 6 373 247 327 0.0 6 324 3 248 291 0.0 6 290 1 249 288 0.0 6 287 1 250 272 0.0 6 269 3 251 240 0.0 6 234 5 0 0 0 0 1 252 278 0.0 6 275 3 253 230 0.0 6 229 1 254 214 0.0 6 213 1 255 222 0.0 6 221 0 1 256 240 0.0 6 237 3 257 244 0.0 6 242 2 258 268 0.0 6 265 3 259 228 0.0 6 225 3 260 166 0.0 6 164 1 0 1 261 192 0.0 6 191 1 262 185 0.0 6 185 263 190 0.0 6 187 2 0 1 264 176 0.0 6 175 1 265 151 0.0 6 149 2 266 129 0.0 6 128 1 267 137 0.0 6 137 268 138 0.0 6 133 5 269 114 0.0 6 113 1 270 88 0.0 6 88 271 114 0.0 6 111 3 272 214 0.0 6 214 273 169 0.0 6 168 1 274 205 0.0 6 203 2 275 228 0.0 6 226 2 276 306 0.0 6 300 6 277 1217 0.0 6 1203 11 1 0 0 0 2 278 1927 0.0 6 1911 15 0 0 0 1 279 32 0.0 6 32 280 3 0.0 6 3 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2455 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 16 369.7 1 16 61 1 0.0 6 0 0 0 1 63 1 0.0 6 1 64 143 0.0 6 139 4 65 1 0.0 6 1 68 1 0.0 6 1 69 19 0.0 6 19 72 1 0.0 6 1 73 9 0.0 6 9 74 17 0.0 6 17 75 16 0.0 6 16 76 14 0.0 6 14 77 17 0.0 6 17 78 20 0.0 6 20 79 20 0.0 6 20 80 20 0.0 6 20 81 29 0.0 6 29 82 16 0.0 6 16 83 21 0.0 6 21 84 11 0.0 6 11 85 15 0.0 6 14 1 86 14 0.0 6 13 1 87 17 0.0 6 16 1 88 11 0.0 6 11 89 7 0.0 6 7 90 22 0.0 6 22 91 19 0.0 6 19 92 19 0.0 6 19 93 12 0.0 6 12 94 15 0.0 6 15 95 16 0.0 6 16 96 13 0.0 6 13 97 8 0.0 6 8 98 19 0.0 6 18 1 99 18 0.0 6 18 100 14 0.0 6 14 101 9 0.0 6 9 102 14 0.0 6 14 103 12 0.0 6 12 104 8 0.0 6 8 105 9 0.0 6 9 106 18 0.0 6 18 107 15 0.0 6 15 108 9 0.0 6 9 109 9 0.0 6 9 110 29 0.0 6 29 111 38 0.0 6 38 112 17 0.0 6 17 113 16 0.0 6 16 114 9 0.0 6 9 115 10 0.0 6 10 116 13 0.0 6 13 117 7 0.0 6 7 118 7 0.0 6 7 119 13 0.0 6 12 1 120 6 0.0 6 6 121 5 0.0 6 5 122 14 0.0 6 14 123 13 0.0 6 13 124 19 0.0 6 19 125 17 0.0 6 17 126 18 0.0 6 18 127 16 0.0 6 16 128 28 0.0 6 28 129 20 0.0 6 20 130 9 0.0 6 9 131 15 0.0 6 15 132 12 0.0 6 12 133 9 0.0 6 9 134 31 0.0 6 31 135 19 0.0 6 19 136 17 0.0 6 17 137 24 0.0 6 24 138 18 0.0 6 18 139 13 0.0 6 13 140 17 0.0 6 17 141 18 0.0 6 18 142 16 0.0 6 15 1 143 11 0.0 6 11 144 26 0.0 6 26 145 12 0.0 6 12 146 13 0.0 6 13 147 15 0.0 6 15 148 11 0.0 6 11 149 14 0.0 6 14 150 21 0.0 6 21 151 14 0.0 6 14 152 12 0.0 6 12 153 16 0.0 6 16 154 16 0.0 6 16 155 17 0.0 6 17 156 17 0.0 6 17 157 16 0.0 6 16 158 15 0.0 6 15 159 17 0.0 6 17 160 15 0.0 6 15 161 15 0.0 6 15 162 17 0.0 6 17 163 22 0.0 6 22 164 17 0.0 6 17 165 21 0.0 6 21 166 22 0.0 6 21 0 0 0 0 0 1 167 21 0.0 6 21 168 20 0.0 6 20 169 13 0.0 6 13 170 22 0.0 6 22 171 20 0.0 6 20 172 22 0.0 6 22 173 6 0.0 6 6 174 10 0.0 6 10 175 15 0.0 6 15 176 15 0.0 6 15 177 20 0.0 6 20 178 7 0.0 6 7 179 11 0.0 6 11 180 14 0.0 6 14 181 16 0.0 6 16 182 15 0.0 6 15 183 25 0.0 6 25 184 7 0.0 6 6 0 0 0 0 0 1 185 13 0.0 6 13 186 18 0.0 6 18 187 6 0.0 6 6 188 8 0.0 6 8 189 16 0.0 6 16 190 13 0.0 6 13 191 12 0.0 6 12 192 10 0.0 6 10 193 15 0.0 6 15 194 14 0.0 6 14 195 12 0.0 6 12 196 6 0.0 6 6 197 4 0.0 6 4 198 10 0.0 6 10 199 4 0.0 6 4 200 8 0.0 6 8 201 17 0.0 6 17 202 17 0.0 6 17 203 13 0.0 6 13 204 17 0.0 6 16 1 205 11 0.0 6 11 206 7 0.0 6 7 207 7 0.0 6 7 208 6 0.0 6 6 209 4 0.0 6 4 210 11 0.0 6 11 211 2 0.0 6 2 212 12 0.0 6 12 213 12 0.0 6 12 214 6 0.0 6 6 215 7 0.0 6 7 216 7 0.0 6 7 217 1 0.0 6 1 218 9 0.0 6 9 219 8 0.0 6 8 221 5 0.0 6 5 222 9 0.0 6 9 223 3 0.0 6 3 224 2 0.0 6 2 225 9 0.0 6 9 226 5 0.0 6 5 227 4 0.0 6 4 228 4 0.0 6 4 229 5 0.0 6 5 230 6 0.0 6 6 231 5 0.0 6 5 232 6 0.0 6 6 233 3 0.0 6 3 234 3 0.0 6 3 235 5 0.0 6 5 236 3 0.0 6 3 237 3 0.0 6 3 238 2 0.0 6 2 239 3 0.0 6 3 240 4 0.0 6 4 241 2 0.0 6 2 242 5 0.0 6 5 243 2 0.0 6 2 244 3 0.0 6 3 246 1 0.0 6 1 247 4 0.0 6 4 248 4 0.0 6 4 249 4 0.0 6 4 250 3 0.0 6 3 251 3 0.0 6 3 252 3 0.0 6 3 253 1 0.0 6 1 255 1 0.0 6 1 256 5 0.0 6 5 258 2 0.0 6 2 260 2 0.0 6 2 261 5 0.0 6 5 262 5 0.0 6 5 263 1 0.0 6 1 264 4 0.0 6 4 265 1 0.0 6 1 266 3 0.0 6 3 269 1 0.0 6 1 270 2 0.0 6 2 271 1 0.0 6 1 273 2 0.0 6 2 274 1 0.0 6 1 276 2 0.0 6 2 278 1 0.0 6 1 279 3 0.0 6 3 281 1 0.0 6 1 283 1 0.0 6 1 285 1 0.0 6 1 296 1 0.0 6 1 301 1 0.0 6 1 305 1 0.0 6 1 306 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 92282 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1478.6 0 1 1 5 1 92.4 1 1 10 8 0.1 3 8 11 3 0.0 3 3 13 11 0.0 3 11 14 9 0.0 4 7 2 15 3 0.0 4 1 2 16 18 0.0 4 11 7 17 37 0.0 5 35 2 18 16 0.0 5 14 1 0 1 19 25 0.0 5 24 1 20 118 0.0 6 115 3 21 60 0.0 6 57 3 22 44 0.0 6 42 1 1 23 37 0.0 6 35 2 24 31 0.0 6 31 25 29 0.0 6 26 3 26 20 0.0 6 20 27 20 0.0 6 19 0 0 0 1 28 25 0.0 6 22 3 29 17 0.0 6 12 3 1 0 0 1 30 18 0.0 6 17 1 31 21 0.0 6 20 1 32 13 0.0 6 12 1 33 19 0.0 6 17 2 34 15 0.0 6 15 35 17 0.0 6 15 2 36 16 0.0 6 14 2 37 17 0.0 6 17 38 16 0.0 6 15 1 39 14 0.0 6 13 1 40 14 0.0 6 13 1 41 19 0.0 6 17 2 42 12 0.0 6 11 1 43 22 0.0 6 19 3 44 20 0.0 6 19 1 45 26 0.0 6 24 2 46 17 0.0 6 14 3 47 24 0.0 6 24 48 29 0.0 6 26 2 0 0 0 0 1 49 30 0.0 6 29 1 50 30 0.0 6 29 1 51 23 0.0 6 22 1 52 24 0.0 6 23 1 53 22 0.0 6 20 2 54 27 0.0 6 24 3 55 25 0.0 6 24 1 56 34 0.0 6 30 3 1 57 24 0.0 6 20 3 1 58 42 0.0 6 39 2 0 0 1 59 36 0.0 6 32 3 1 60 39 0.0 6 36 3 61 26 0.0 6 24 1 1 62 27 0.0 6 24 2 0 0 0 1 63 49 0.0 6 44 3 0 1 0 0 1 64 49 0.0 6 47 1 1 65 47 0.0 6 45 2 66 58 0.0 6 57 1 67 159 0.0 6 147 9 1 2 68 82 0.0 6 77 3 0 0 2 69 93 0.0 6 91 2 70 53 0.0 6 49 4 71 76 0.0 6 71 3 0 0 1 0 1 72 49 0.0 6 45 4 73 55 0.0 6 52 2 0 0 0 0 1 74 52 0.0 6 48 4 75 56 0.0 6 51 5 76 50 0.0 6 46 3 1 77 51 0.0 6 46 4 1 78 57 0.0 6 51 6 79 76 0.0 6 73 3 80 77 0.0 6 73 3 0 0 0 1 81 56 0.0 6 53 3 82 81 0.0 6 74 5 1 1 83 92 0.0 6 85 4 0 0 1 1 1 84 60 0.0 6 57 3 85 66 0.0 6 65 1 86 59 0.0 6 56 3 87 62 0.0 6 58 3 0 0 0 1 88 70 0.0 6 69 1 89 72 0.0 6 70 2 90 94 0.0 6 91 3 91 104 0.0 6 100 4 92 81 0.0 6 77 4 93 121 0.0 6 115 4 0 0 1 1 94 104 0.0 6 102 2 95 114 0.0 6 107 4 2 0 0 1 96 117 0.0 6 111 5 1 97 123 0.0 6 120 3 98 120 0.0 6 117 2 0 1 99 116 0.0 6 111 4 1 100 107 0.0 6 104 3 101 129 0.0 6 125 3 0 0 0 1 102 122 0.0 6 118 3 1 103 164 0.0 6 155 9 104 247 0.0 6 237 8 1 0 0 1 105 144 0.0 6 138 3 0 0 2 1 106 161 0.0 6 154 6 1 107 166 0.0 6 157 8 0 0 1 108 166 0.0 6 158 5 0 0 0 1 2 109 180 0.0 6 175 5 110 172 0.0 6 169 1 0 0 1 1 111 203 0.0 6 197 5 0 0 0 1 112 243 0.0 6 241 2 113 201 0.0 6 195 6 114 213 0.0 6 206 7 115 222 0.0 6 217 4 0 0 0 1 116 231 0.0 6 227 4 117 234 0.0 6 226 8 118 290 0.0 6 287 3 119 266 0.0 6 260 3 2 0 0 1 120 254 0.0 6 248 4 0 0 1 1 121 265 0.0 6 258 6 0 0 0 1 122 290 0.0 6 286 4 123 292 0.0 6 287 3 0 0 1 1 124 311 0.0 6 302 7 0 0 1 1 125 433 0.0 6 427 4 0 0 0 2 126 411 0.0 6 399 12 127 463 0.0 6 451 10 0 0 0 1 1 128 507 0.0 6 499 6 0 0 1 0 1 129 511 0.0 6 503 4 1 0 0 2 1 130 570 0.0 6 564 5 0 0 1 131 606 0.0 6 592 11 1 0 0 0 2 132 679 0.0 6 667 11 0 0 1 133 679 0.0 6 671 6 0 1 0 1 134 656 0.0 6 646 7 0 1 0 0 2 135 713 0.0 6 703 10 136 648 0.0 6 640 6 0 0 0 0 2 137 736 0.0 6 722 12 0 0 1 0 1 138 745 0.0 6 735 9 0 0 0 0 1 139 792 0.0 6 780 8 0 3 0 1 140 889 0.0 6 876 12 1 141 1095 0.0 6 1078 12 1 1 0 1 2 142 1430 0.0 6 1416 10 1 1 1 0 1 143 1465 0.0 6 1447 16 1 0 1 144 1423 0.0 6 1412 9 1 1 145 1485 0.0 6 1466 18 0 1 146 1695 0.0 6 1675 17 2 0 1 147 1645 0.0 6 1630 13 0 1 0 0 1 148 1862 0.0 6 1843 15 2 0 1 1 149 2008 0.0 6 1991 15 0 0 1 0 1 150 2561 0.0 6 2536 23 0 1 0 0 1 151 1984 0.0 6 1957 24 0 2 0 0 1 152 2178 0.0 6 2149 25 1 1 2 153 1998 0.0 6 1975 18 0 1 2 0 2 154 1685 0.0 6 1675 9 0 1 155 1780 0.0 6 1756 22 1 0 0 1 156 1652 0.0 6 1632 18 1 0 0 0 1 157 1791 0.0 6 1772 17 0 0 1 1 158 1833 0.0 6 1814 16 1 1 0 0 1 159 1640 0.0 6 1629 10 0 1 160 1610 0.0 6 1598 9 1 1 1 161 1403 0.0 6 1387 15 1 162 1311 0.0 6 1298 10 0 0 1 0 2 163 1355 0.0 6 1351 3 1 164 1262 0.0 6 1251 7 1 0 0 2 1 165 1187 0.0 6 1180 7 166 1519 0.0 6 1506 11 0 1 0 1 167 1247 0.0 6 1239 6 1 0 1 168 1430 0.0 6 1419 9 1 0 0 0 1 169 1217 0.0 6 1207 9 1 170 1232 0.0 6 1225 6 0 0 0 1 171 1384 0.0 6 1373 10 0 1 172 1306 0.0 6 1296 9 0 0 1 173 1287 0.0 6 1283 2 1 0 1 174 1158 0.0 6 1153 4 0 1 175 1179 0.0 6 1169 9 0 0 1 176 1274 0.0 6 1264 9 0 0 0 0 1 177 987 0.0 6 984 2 1 178 1040 0.0 6 1034 5 0 0 0 1 179 893 0.0 6 883 9 0 0 1 180 740 0.0 6 736 4 181 708 0.0 6 706 2 182 650 0.0 6 639 8 1 1 0 0 1 183 890 0.0 6 885 4 0 0 0 1 184 1064 0.0 6 1059 4 0 0 0 0 1 185 985 0.0 6 979 6 186 699 0.0 6 691 5 1 1 0 0 1 187 674 0.0 6 666 7 0 1 188 634 0.0 6 631 3 189 553 0.0 6 546 6 1 190 537 0.0 6 529 7 0 0 0 1 191 608 0.0 6 603 3 1 0 1 192 378 0.0 6 375 3 193 351 0.0 6 348 3 194 324 0.0 6 318 4 1 0 0 0 1 195 323 0.0 6 320 2 0 0 1 196 459 0.0 6 451 8 197 296 0.0 6 294 2 198 319 0.0 6 312 7 199 307 0.0 6 303 4 200 220 0.0 6 217 2 1 201 211 0.0 6 210 0 0 0 0 0 1 202 194 0.0 6 193 1 203 166 0.0 6 163 3 204 162 0.0 6 161 0 0 0 0 1 205 141 0.0 6 138 3 206 124 0.0 6 124 207 113 0.0 6 111 2 208 108 0.0 6 106 2 209 83 0.0 6 81 2 210 79 0.0 6 79 211 73 0.0 6 72 1 212 116 0.0 6 114 2 213 82 0.0 6 81 1 214 109 0.0 6 108 1 215 70 0.0 6 67 2 0 1 216 63 0.0 6 62 1 217 63 0.0 6 62 1 218 43 0.0 6 43 219 36 0.0 6 34 1 0 0 1 220 31 0.0 6 30 1 221 39 0.0 6 38 0 1 222 39 0.0 6 38 1 223 50 0.0 6 49 1 224 32 0.0 6 31 1 225 35 0.0 6 34 0 0 0 1 226 32 0.0 6 31 1 227 22 0.0 6 21 1 228 19 0.0 6 19 229 14 0.0 6 14 230 15 0.0 6 15 231 18 0.0 6 18 232 23 0.0 6 22 1 233 7 0.0 6 6 1 234 9 0.0 6 9 235 5 0.0 6 4 1 236 3 0.0 6 3 239 4 0.0 6 4 240 8 0.0 6 8 241 9 0.0 6 9 242 9 0.0 6 9 243 8 0.0 6 8 244 5 0.0 6 5 245 2 0.0 6 2 246 6 0.0 6 6 248 3 0.0 6 3 249 2 0.0 6 2 251 2 0.0 6 2 276 2 0.0 6 2 277 1 0.0 6 1 278 10 0.0 6 10 279 68 0.0 6 67 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2277 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 52 1 0.0 6 1 54 1 0.0 6 1 55 2 0.0 6 2 56 1 0.0 6 1 57 1 0.0 6 1 59 2 0.0 6 2 62 1 0.0 6 1 65 1 0.0 6 1 68 10 0.0 6 10 71 2 0.0 6 2 72 7 0.0 6 7 73 22 0.0 6 22 74 25 0.0 6 25 75 18 0.0 6 17 1 76 21 0.0 6 21 77 28 0.0 6 28 78 26 0.0 6 26 79 28 0.0 6 27 1 80 24 0.0 6 24 81 31 0.0 6 31 82 28 0.0 6 28 83 16 0.0 6 16 84 44 0.0 6 44 85 15 0.0 6 14 1 86 24 0.0 6 24 87 10 0.0 6 10 88 12 0.0 6 12 89 21 0.0 6 20 1 90 12 0.0 6 12 91 14 0.0 6 13 1 92 22 0.0 6 22 93 17 0.0 6 17 94 17 0.0 6 17 95 15 0.0 6 15 96 14 0.0 6 14 97 19 0.0 6 19 98 10 0.0 6 10 99 10 0.0 6 10 100 17 0.0 6 17 101 12 0.0 6 12 102 36 0.0 6 35 1 103 16 0.0 6 15 0 0 0 1 104 17 0.0 6 16 0 0 0 1 105 15 0.0 6 15 106 19 0.0 6 19 107 17 0.0 6 17 108 8 0.0 6 8 109 29 0.0 6 29 110 58 0.0 6 58 111 21 0.0 6 21 112 16 0.0 6 16 113 23 0.0 6 23 114 12 0.0 6 12 115 16 0.0 6 15 0 0 1 116 8 0.0 6 8 117 15 0.0 6 15 118 9 0.0 6 9 119 14 0.0 6 14 120 15 0.0 6 15 121 18 0.0 6 17 0 0 0 1 122 17 0.0 6 17 123 20 0.0 6 20 124 15 0.0 6 15 125 21 0.0 6 21 126 15 0.0 6 15 127 11 0.0 6 11 128 39 0.0 6 39 129 15 0.0 6 15 130 25 0.0 6 25 131 27 0.0 6 27 132 12 0.0 6 12 133 27 0.0 6 27 134 34 0.0 6 34 135 17 0.0 6 17 136 27 0.0 6 26 1 137 27 0.0 6 27 138 19 0.0 6 18 1 139 15 0.0 6 15 140 29 0.0 6 29 141 18 0.0 6 18 142 11 0.0 6 11 143 24 0.0 6 24 144 19 0.0 6 19 145 16 0.0 6 16 146 17 0.0 6 17 147 19 0.0 6 19 148 18 0.0 6 18 149 22 0.0 6 22 150 17 0.0 6 17 151 11 0.0 6 11 152 16 0.0 6 15 1 153 18 0.0 6 18 154 16 0.0 6 16 155 21 0.0 6 21 156 35 0.0 6 35 157 13 0.0 6 13 158 21 0.0 6 21 159 18 0.0 6 18 160 18 0.0 6 18 161 13 0.0 6 13 162 26 0.0 6 26 163 19 0.0 6 19 164 27 0.0 6 26 0 0 0 1 165 22 0.0 6 22 166 20 0.0 6 20 167 27 0.0 6 27 168 18 0.0 6 18 169 19 0.0 6 19 170 18 0.0 6 18 171 12 0.0 6 12 172 21 0.0 6 21 173 8 0.0 6 8 174 10 0.0 6 10 175 13 0.0 6 13 176 13 0.0 6 13 177 12 0.0 6 12 178 10 0.0 6 10 179 11 0.0 6 11 180 15 0.0 6 15 181 3 0.0 6 3 182 5 0.0 6 5 183 12 0.0 6 12 184 9 0.0 6 9 185 15 0.0 6 15 186 7 0.0 6 7 187 8 0.0 6 8 188 8 0.0 6 8 189 15 0.0 6 15 190 9 0.0 6 9 191 6 0.0 6 6 192 5 0.0 6 5 193 5 0.0 6 4 1 194 3 0.0 6 3 195 3 0.0 6 3 196 3 0.0 6 3 197 5 0.0 6 5 198 6 0.0 6 6 199 3 0.0 6 3 200 2 0.0 6 1 1 201 2 0.0 6 2 202 4 0.0 6 4 203 5 0.0 6 5 204 9 0.0 6 9 205 1 0.0 6 1 206 3 0.0 6 2 0 0 0 1 207 4 0.0 6 4 208 2 0.0 6 2 211 4 0.0 6 4 212 1 0.0 6 1 214 2 0.0 6 2 216 3 0.0 6 3 217 1 0.0 6 1 218 1 0.0 6 1 225 1 0.0 6 1 232 1 0.0 6 1 234 2 0.0 6 2 235 1 0.0 6 1 245 1 0.0 6 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_08_186_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_08_413_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_08_186_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_08_413_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.92 s (32 µs/read; 1.85 M reads/minute). === Summary === Total read pairs processed: 121,097 Read 1 with adapter: 121,097 (100.0%) Read 2 with adapter: 121,097 (100.0%) == Read fate breakdown == Pairs that were too short: 28 (0.0%) Pairs discarded as untrimmed: 0 (0.0%) Pairs written (passing filters): 121,069 (100.0%) Total basepairs processed: 53,758,110 bp Read 1: 29,827,493 bp Read 2: 23,930,617 bp Total written (filtered): 7,952,982 bp (14.8%) Read 1: 4,117,710 bp Read 2: 3,835,272 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 118849 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 9 473.0 1 9 5 2 118.3 1 2 6 4 29.6 1 0 0 4 7 7 7.4 2 4 0 3 9 1 0.5 2 1 16 3 0.0 4 3 17 5 0.0 5 5 18 2 0.0 5 2 19 4 0.0 5 3 1 20 105 0.0 6 102 3 21 79 0.0 6 79 22 50 0.0 6 50 23 36 0.0 6 36 24 27 0.0 6 23 3 1 25 19 0.0 6 19 26 28 0.0 6 23 4 0 0 0 0 1 27 10 0.0 6 10 28 10 0.0 6 9 1 29 13 0.0 6 13 30 8 0.0 6 5 3 31 16 0.0 6 15 1 32 6 0.0 6 5 1 33 16 0.0 6 15 1 34 6 0.0 6 5 1 35 5 0.0 6 5 36 7 0.0 6 7 37 7 0.0 6 7 38 8 0.0 6 6 1 1 39 10 0.0 6 10 40 15 0.0 6 14 1 41 6 0.0 6 5 1 42 7 0.0 6 7 43 4 0.0 6 4 44 7 0.0 6 7 45 12 0.0 6 10 1 0 1 46 7 0.0 6 7 47 10 0.0 6 8 1 0 1 48 8 0.0 6 7 1 49 10 0.0 6 10 50 9 0.0 6 9 51 10 0.0 6 9 1 52 9 0.0 6 8 0 0 1 53 10 0.0 6 9 1 54 5 0.0 6 4 0 0 1 55 5 0.0 6 5 56 3 0.0 6 3 57 8 0.0 6 7 1 58 6 0.0 6 4 1 1 59 6 0.0 6 6 60 6 0.0 6 6 61 7 0.0 6 7 62 5 0.0 6 5 63 11 0.0 6 10 1 64 14 0.0 6 14 65 8 0.0 6 7 0 0 0 0 1 66 13 0.0 6 12 1 67 13 0.0 6 12 1 68 11 0.0 6 10 1 69 20 0.0 6 20 70 5 0.0 6 5 71 13 0.0 6 13 72 13 0.0 6 13 73 8 0.0 6 6 2 74 12 0.0 6 11 1 75 11 0.0 6 11 76 10 0.0 6 9 1 77 14 0.0 6 14 78 12 0.0 6 11 1 79 13 0.0 6 13 80 15 0.0 6 15 81 10 0.0 6 9 1 82 12 0.0 6 12 83 9 0.0 6 9 84 12 0.0 6 12 85 9 0.0 6 8 1 86 19 0.0 6 19 87 10 0.0 6 9 1 88 13 0.0 6 13 89 9 0.0 6 9 90 16 0.0 6 16 91 10 0.0 6 9 0 0 0 1 92 15 0.0 6 15 93 13 0.0 6 11 2 94 8 0.0 6 8 95 17 0.0 6 17 96 10 0.0 6 9 1 97 17 0.0 6 17 98 13 0.0 6 13 99 10 0.0 6 10 100 12 0.0 6 12 101 20 0.0 6 20 102 28 0.0 6 27 1 103 18 0.0 6 16 2 104 17 0.0 6 17 105 19 0.0 6 18 1 106 16 0.0 6 16 107 15 0.0 6 14 1 108 22 0.0 6 22 109 29 0.0 6 29 110 17 0.0 6 17 111 32 0.0 6 31 1 112 33 0.0 6 32 1 113 21 0.0 6 21 114 40 0.0 6 40 115 32 0.0 6 31 1 116 33 0.0 6 32 1 117 32 0.0 6 32 118 37 0.0 6 36 1 119 40 0.0 6 37 1 0 0 0 1 1 120 29 0.0 6 29 121 33 0.0 6 33 122 48 0.0 6 47 1 123 39 0.0 6 38 0 0 1 124 41 0.0 6 40 1 125 36 0.0 6 36 126 47 0.0 6 43 3 0 0 0 0 1 127 52 0.0 6 51 1 128 67 0.0 6 67 129 50 0.0 6 50 130 57 0.0 6 56 1 131 44 0.0 6 44 132 57 0.0 6 55 2 133 68 0.0 6 67 1 134 89 0.0 6 86 3 135 85 0.0 6 83 2 136 70 0.0 6 69 1 137 63 0.0 6 62 0 1 138 80 0.0 6 77 3 139 93 0.0 6 92 1 140 121 0.0 6 121 141 139 0.0 6 138 1 142 372 0.0 6 372 143 676 0.0 6 672 3 1 144 976 0.0 6 975 1 145 665 0.0 6 661 4 146 544 0.0 6 544 147 857 0.0 6 857 148 801 0.0 6 797 3 1 149 686 0.0 6 684 1 0 0 0 0 1 150 760 0.0 6 757 3 151 745 0.0 6 740 5 152 728 0.0 6 728 153 695 0.0 6 692 3 154 547 0.0 6 547 155 512 0.0 6 511 1 156 618 0.0 6 617 1 157 535 0.0 6 533 2 158 456 0.0 6 453 3 159 709 0.0 6 707 1 0 0 0 0 1 160 645 0.0 6 645 161 591 0.0 6 590 1 162 625 0.0 6 624 1 163 663 0.0 6 659 4 164 523 0.0 6 521 2 165 692 0.0 6 689 3 166 595 0.0 6 592 3 167 508 0.0 6 507 1 168 620 0.0 6 619 1 169 619 0.0 6 615 3 0 1 170 651 0.0 6 649 1 0 0 0 0 1 171 796 0.0 6 793 3 172 698 0.0 6 696 2 173 552 0.0 6 552 174 638 0.0 6 637 1 175 663 0.0 6 663 176 564 0.0 6 564 177 742 0.0 6 737 5 178 710 0.0 6 706 4 179 589 0.0 6 586 3 180 996 0.0 6 994 2 181 720 0.0 6 717 3 182 659 0.0 6 659 183 673 0.0 6 672 1 184 664 0.0 6 661 3 185 637 0.0 6 636 1 186 675 0.0 6 673 2 187 632 0.0 6 628 4 188 653 0.0 6 652 1 189 774 0.0 6 773 1 190 814 0.0 6 811 3 191 763 0.0 6 757 6 192 850 0.0 6 847 3 193 863 0.0 6 859 4 194 842 0.0 6 839 3 195 1060 0.0 6 1058 2 196 1076 0.0 6 1069 6 0 0 0 1 197 1169 0.0 6 1164 4 0 0 0 0 1 198 1110 0.0 6 1106 4 199 848 0.0 6 844 4 200 897 0.0 6 892 5 201 882 0.0 6 876 6 202 938 0.0 6 937 1 203 861 0.0 6 858 2 0 0 0 1 204 1251 0.0 6 1247 2 1 0 0 1 205 1236 0.0 6 1229 7 206 1129 0.0 6 1121 7 1 207 1164 0.0 6 1154 10 208 1095 0.0 6 1080 15 209 928 0.0 6 924 4 210 1135 0.0 6 1123 10 1 0 0 1 211 1434 0.0 6 1422 11 1 212 900 0.0 6 892 7 0 1 213 1503 0.0 6 1493 10 214 1214 0.0 6 1206 8 215 976 0.0 6 964 10 2 216 992 0.0 6 985 7 217 1088 0.0 6 1075 13 218 834 0.0 6 831 3 219 856 0.0 6 851 5 220 740 0.0 6 732 7 0 0 0 0 1 221 823 0.0 6 820 3 222 852 0.0 6 846 6 223 978 0.0 6 973 5 224 1054 0.0 6 1048 6 225 984 0.0 6 966 17 1 226 1035 0.0 6 1028 7 227 887 0.0 6 878 9 228 846 0.0 6 841 5 229 799 0.0 6 793 6 230 743 0.0 6 736 7 231 733 0.0 6 725 8 232 753 0.0 6 744 7 2 233 663 0.0 6 660 3 234 666 0.0 6 657 9 235 565 0.0 6 559 6 236 628 0.0 6 620 8 237 1157 0.0 6 1143 14 238 561 0.0 6 557 3 1 239 603 0.0 6 597 6 240 593 0.0 6 589 4 241 501 0.0 6 494 7 242 495 0.0 6 493 2 243 697 0.0 6 687 10 244 780 0.0 6 767 13 245 452 0.0 6 446 6 246 342 0.0 6 337 4 0 1 247 584 0.0 6 578 5 0 0 1 248 406 0.0 6 392 14 249 1292 0.0 6 1266 23 1 1 1 250 513 0.0 6 504 9 251 422 0.0 6 415 6 1 252 675 0.0 6 648 25 2 253 660 0.0 6 639 20 1 254 1081 0.0 6 1051 28 2 255 589 0.0 6 570 17 2 256 1372 0.0 6 1340 30 2 257 549 0.0 6 519 29 0 1 258 1344 0.0 6 1312 32 259 577 0.0 6 555 19 1 1 0 0 1 260 890 0.0 6 866 24 261 374 0.0 6 368 6 262 350 0.0 6 343 7 263 577 0.0 6 561 16 264 824 0.0 6 810 13 1 265 306 0.0 6 298 8 266 679 0.0 6 664 15 267 419 0.0 6 406 12 1 268 754 0.0 6 744 10 269 426 0.0 6 417 9 270 218 0.0 6 212 6 271 247 0.0 6 246 1 272 515 0.0 6 500 15 273 1029 0.0 6 992 35 2 274 1245 0.0 6 1216 29 275 1983 0.0 6 1920 58 5 276 3366 0.0 6 3274 89 2 1 277 5973 0.0 6 5838 131 4 278 3233 0.0 6 3171 60 2 279 37 0.0 6 35 1 1 280 5 0.0 6 3 1 0 1 281 1 0.0 6 0 0 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2248 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 11 473.0 1 11 63 3 0.0 6 3 64 128 0.0 6 121 7 65 1 0.0 6 0 1 70 4 0.0 6 4 72 3 0.0 6 3 73 12 0.0 6 12 74 29 0.0 6 29 75 31 0.0 6 31 76 27 0.0 6 27 77 19 0.0 6 19 78 29 0.0 6 29 79 26 0.0 6 26 80 23 0.0 6 23 81 33 0.0 6 33 82 17 0.0 6 17 83 19 0.0 6 19 84 22 0.0 6 22 85 13 0.0 6 13 86 10 0.0 6 10 87 19 0.0 6 19 88 7 0.0 6 7 89 21 0.0 6 21 90 34 0.0 6 34 91 17 0.0 6 17 92 14 0.0 6 14 93 20 0.0 6 20 94 13 0.0 6 13 95 12 0.0 6 12 96 19 0.0 6 19 97 17 0.0 6 16 1 98 13 0.0 6 13 99 8 0.0 6 8 100 14 0.0 6 14 101 22 0.0 6 22 102 20 0.0 6 20 103 11 0.0 6 11 104 13 0.0 6 13 105 14 0.0 6 14 106 15 0.0 6 15 107 24 0.0 6 24 108 19 0.0 6 19 109 11 0.0 6 11 110 16 0.0 6 16 111 28 0.0 6 28 112 16 0.0 6 16 113 15 0.0 6 15 114 15 0.0 6 15 115 15 0.0 6 14 1 116 10 0.0 6 10 117 18 0.0 6 18 118 15 0.0 6 15 119 22 0.0 6 22 120 17 0.0 6 17 121 16 0.0 6 16 122 9 0.0 6 9 123 14 0.0 6 13 1 124 21 0.0 6 21 125 13 0.0 6 13 126 19 0.0 6 19 127 15 0.0 6 15 128 17 0.0 6 17 129 16 0.0 6 16 130 18 0.0 6 18 131 13 0.0 6 12 1 132 13 0.0 6 12 1 133 17 0.0 6 17 134 23 0.0 6 23 135 31 0.0 6 31 136 15 0.0 6 15 137 15 0.0 6 15 138 19 0.0 6 19 139 10 0.0 6 10 140 16 0.0 6 16 141 15 0.0 6 15 142 22 0.0 6 22 143 14 0.0 6 14 144 12 0.0 6 12 145 11 0.0 6 11 146 15 0.0 6 15 147 9 0.0 6 9 148 21 0.0 6 21 149 11 0.0 6 11 150 13 0.0 6 13 151 18 0.0 6 18 152 13 0.0 6 13 153 28 0.0 6 28 154 7 0.0 6 7 155 13 0.0 6 13 156 20 0.0 6 20 157 20 0.0 6 20 158 18 0.0 6 18 159 19 0.0 6 19 160 10 0.0 6 10 161 17 0.0 6 17 162 13 0.0 6 13 163 6 0.0 6 6 164 7 0.0 6 7 165 16 0.0 6 16 166 11 0.0 6 11 167 13 0.0 6 13 168 12 0.0 6 12 169 16 0.0 6 16 170 14 0.0 6 14 171 10 0.0 6 10 172 9 0.0 6 9 173 10 0.0 6 9 1 174 15 0.0 6 15 175 17 0.0 6 17 176 12 0.0 6 12 177 4 0.0 6 3 0 0 0 0 0 1 178 7 0.0 6 7 179 6 0.0 6 6 180 8 0.0 6 8 181 12 0.0 6 12 182 7 0.0 6 7 183 5 0.0 6 5 184 5 0.0 6 5 185 9 0.0 6 9 186 10 0.0 6 10 187 6 0.0 6 6 188 11 0.0 6 11 189 9 0.0 6 9 190 10 0.0 6 10 191 13 0.0 6 13 192 8 0.0 6 8 193 13 0.0 6 13 194 4 0.0 6 4 195 4 0.0 6 4 196 5 0.0 6 5 197 4 0.0 6 4 198 8 0.0 6 7 1 199 16 0.0 6 16 200 5 0.0 6 5 201 9 0.0 6 9 202 6 0.0 6 6 203 7 0.0 6 7 204 10 0.0 6 10 205 5 0.0 6 5 206 5 0.0 6 5 207 2 0.0 6 2 208 6 0.0 6 6 209 7 0.0 6 7 210 3 0.0 6 3 211 5 0.0 6 5 212 7 0.0 6 6 1 213 3 0.0 6 3 214 6 0.0 6 6 215 3 0.0 6 3 216 4 0.0 6 4 217 2 0.0 6 2 218 4 0.0 6 4 219 5 0.0 6 5 220 2 0.0 6 2 221 3 0.0 6 3 222 5 0.0 6 5 224 4 0.0 6 4 225 3 0.0 6 3 226 5 0.0 6 5 229 3 0.0 6 3 230 5 0.0 6 5 231 4 0.0 6 4 232 3 0.0 6 3 233 1 0.0 6 1 234 3 0.0 6 3 235 2 0.0 6 2 236 4 0.0 6 4 237 5 0.0 6 5 238 4 0.0 6 4 239 1 0.0 6 1 240 2 0.0 6 2 241 1 0.0 6 1 243 2 0.0 6 2 244 3 0.0 6 3 245 1 0.0 6 1 246 3 0.0 6 3 247 4 0.0 6 4 248 2 0.0 6 2 249 2 0.0 6 2 250 4 0.0 6 4 253 1 0.0 6 1 255 2 0.0 6 2 258 1 0.0 6 1 259 3 0.0 6 3 261 2 0.0 6 2 266 2 0.0 6 2 267 1 0.0 6 1 271 2 0.0 6 2 272 1 0.0 6 1 273 1 0.0 6 1 289 1 0.0 6 1 295 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 118647 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 29.6 1 1 7 1 7.4 2 1 9 1 0.5 2 1 10 2 0.1 3 2 11 11 0.0 3 9 2 12 16 0.0 3 14 1 0 1 13 13 0.0 3 13 14 14 0.0 4 13 1 15 4 0.0 4 3 1 16 8 0.0 4 8 17 36 0.0 5 35 1 18 30 0.0 5 26 4 19 18 0.0 5 14 4 20 110 0.0 6 102 8 21 76 0.0 6 71 5 22 61 0.0 6 57 4 23 34 0.0 6 31 1 0 0 1 0 1 24 22 0.0 6 19 3 25 24 0.0 6 21 3 26 26 0.0 6 23 3 27 29 0.0 6 27 2 28 29 0.0 6 27 2 29 22 0.0 6 22 30 12 0.0 6 12 31 19 0.0 6 16 3 32 19 0.0 6 17 2 33 11 0.0 6 9 1 0 1 34 14 0.0 6 13 1 35 17 0.0 6 15 2 36 19 0.0 6 18 1 37 15 0.0 6 13 2 38 14 0.0 6 13 1 39 22 0.0 6 20 1 1 40 16 0.0 6 14 2 41 16 0.0 6 13 3 42 13 0.0 6 11 2 43 18 0.0 6 18 44 25 0.0 6 22 3 45 9 0.0 6 7 1 1 46 25 0.0 6 21 3 1 47 21 0.0 6 17 4 48 23 0.0 6 20 3 49 30 0.0 6 29 1 50 29 0.0 6 22 6 0 1 51 25 0.0 6 19 6 52 41 0.0 6 37 3 0 0 1 53 23 0.0 6 21 2 54 26 0.0 6 23 2 0 0 1 55 23 0.0 6 19 3 1 56 27 0.0 6 26 0 1 57 32 0.0 6 26 5 0 0 1 58 21 0.0 6 18 2 0 1 59 27 0.0 6 24 3 60 20 0.0 6 19 1 61 31 0.0 6 29 1 0 1 62 27 0.0 6 23 3 0 1 63 29 0.0 6 27 2 64 38 0.0 6 35 3 65 31 0.0 6 27 4 66 38 0.0 6 32 3 1 0 1 1 67 73 0.0 6 67 4 1 0 0 0 1 68 201 0.0 6 194 7 69 58 0.0 6 55 3 70 108 0.0 6 106 2 71 66 0.0 6 61 4 1 72 84 0.0 6 75 7 1 0 0 1 73 44 0.0 6 43 1 74 30 0.0 6 27 2 0 0 1 75 42 0.0 6 38 4 76 50 0.0 6 47 2 1 77 41 0.0 6 38 3 78 51 0.0 6 47 3 0 0 0 0 1 79 59 0.0 6 57 2 80 62 0.0 6 60 1 0 0 0 1 81 59 0.0 6 55 4 82 79 0.0 6 76 3 83 90 0.0 6 86 4 84 117 0.0 6 113 4 85 66 0.0 6 61 5 86 48 0.0 6 44 4 87 73 0.0 6 70 1 1 1 88 56 0.0 6 51 4 1 89 72 0.0 6 69 2 0 1 90 78 0.0 6 77 1 91 91 0.0 6 85 6 92 89 0.0 6 87 2 93 91 0.0 6 88 3 94 146 0.0 6 136 7 2 0 1 95 82 0.0 6 80 2 96 96 0.0 6 93 2 1 97 113 0.0 6 112 0 0 0 0 1 98 118 0.0 6 113 4 1 99 101 0.0 6 98 3 100 95 0.0 6 91 3 0 0 1 101 109 0.0 6 107 2 102 128 0.0 6 123 5 103 105 0.0 6 103 1 0 1 104 165 0.0 6 155 9 1 105 239 0.0 6 235 3 0 0 0 1 106 156 0.0 6 152 4 107 154 0.0 6 150 3 0 1 108 130 0.0 6 121 8 0 1 109 155 0.0 6 152 3 110 157 0.0 6 153 2 1 1 111 151 0.0 6 148 2 0 0 0 0 1 112 176 0.0 6 170 5 0 0 1 113 169 0.0 6 166 3 114 178 0.0 6 172 5 1 115 183 0.0 6 178 5 116 216 0.0 6 210 4 0 0 2 117 199 0.0 6 196 2 0 0 0 1 118 236 0.0 6 230 5 0 1 119 271 0.0 6 263 6 1 0 0 1 120 251 0.0 6 248 3 121 225 0.0 6 220 5 122 219 0.0 6 214 5 123 212 0.0 6 208 2 1 0 0 0 1 124 257 0.0 6 254 1 2 125 321 0.0 6 319 1 0 0 0 1 126 364 0.0 6 356 8 127 405 0.0 6 401 4 128 379 0.0 6 374 5 129 379 0.0 6 376 2 0 0 0 0 1 130 426 0.0 6 421 4 0 0 0 0 1 131 529 0.0 6 521 6 1 0 0 1 132 618 0.0 6 607 10 1 133 560 0.0 6 546 11 0 2 0 1 134 598 0.0 6 583 12 3 135 750 0.0 6 743 7 136 617 0.0 6 606 11 137 635 0.0 6 622 12 0 1 138 587 0.0 6 580 6 0 0 0 1 139 702 0.0 6 697 5 140 853 0.0 6 842 11 141 1039 0.0 6 1026 12 0 0 1 142 1377 0.0 6 1355 21 0 1 143 1712 0.0 6 1702 9 0 0 0 0 1 144 1469 0.0 6 1457 11 0 1 145 1432 0.0 6 1421 7 0 2 1 1 146 1543 0.0 6 1533 9 0 0 0 1 147 1562 0.0 6 1555 6 0 1 148 1463 0.0 6 1445 15 0 0 1 0 2 149 1854 0.0 6 1840 9 3 0 1 1 150 1803 0.0 6 1782 16 2 0 2 0 1 151 2339 0.0 6 2327 9 1 2 152 1874 0.0 6 1858 13 2 0 1 153 1923 0.0 6 1906 15 0 0 0 0 2 154 1992 0.0 6 1983 6 1 1 1 155 1582 0.0 6 1563 16 0 2 0 0 1 156 1665 0.0 6 1650 13 0 0 1 0 1 157 1622 0.0 6 1605 13 0 2 1 1 158 1897 0.0 6 1879 16 1 0 0 0 1 159 1708 0.0 6 1699 7 1 0 0 0 1 160 1592 0.0 6 1579 8 0 1 2 1 1 161 1540 0.0 6 1528 12 162 1481 0.0 6 1465 14 2 163 1280 0.0 6 1270 8 1 0 0 1 164 1469 0.0 6 1453 15 1 165 1396 0.0 6 1377 16 2 0 0 1 166 1461 0.0 6 1435 23 1 1 1 167 1618 0.0 6 1594 22 1 0 0 1 168 1635 0.0 6 1617 17 0 0 1 169 1485 0.0 6 1465 19 1 170 1476 0.0 6 1459 17 171 1440 0.0 6 1423 15 2 172 1321 0.0 6 1312 8 0 0 0 1 173 1386 0.0 6 1370 14 0 1 0 1 174 1500 0.0 6 1483 16 0 0 0 0 1 175 1675 0.0 6 1648 24 1 0 1 1 176 1306 0.0 6 1283 21 0 1 1 177 1666 0.0 6 1641 22 2 0 0 0 1 178 1234 0.0 6 1211 21 0 1 1 179 1438 0.0 6 1415 22 1 180 1039 0.0 6 1026 12 0 0 1 181 1324 0.0 6 1310 12 1 1 182 810 0.0 6 795 12 0 0 1 1 1 183 1138 0.0 6 1121 17 184 1319 0.0 6 1300 16 0 1 2 185 1410 0.0 6 1386 21 2 0 0 0 1 186 1878 0.0 6 1844 33 0 0 1 187 1420 0.0 6 1387 30 0 0 2 0 1 188 1613 0.0 6 1579 33 1 189 1003 0.0 6 980 22 0 0 1 190 1112 0.0 6 1086 26 191 1017 0.0 6 989 26 2 192 969 0.0 6 941 27 1 193 1255 0.0 6 1222 33 194 634 0.0 6 620 12 0 1 0 1 195 973 0.0 6 951 21 0 1 196 680 0.0 6 665 14 0 1 197 898 0.0 6 875 20 3 198 1479 0.0 6 1446 31 0 0 2 199 1103 0.0 6 1069 33 0 0 1 200 739 0.0 6 721 16 1 0 1 201 798 0.0 6 778 20 202 651 0.0 6 633 16 1 1 203 571 0.0 6 559 10 1 0 0 0 1 204 435 0.0 6 419 16 205 693 0.0 6 668 24 1 206 213 0.0 6 203 8 2 207 455 0.0 6 443 12 208 787 0.0 6 767 19 0 1 209 522 0.0 6 502 20 210 242 0.0 6 236 4 0 1 1 211 363 0.0 6 347 15 1 212 480 0.0 6 471 9 213 610 0.0 6 593 16 0 0 1 214 307 0.0 6 299 6 1 0 1 215 346 0.0 6 340 6 216 296 0.0 6 288 8 217 260 0.0 6 248 11 1 218 219 0.0 6 208 11 219 184 0.0 6 180 3 0 0 1 220 77 0.0 6 74 3 221 60 0.0 6 57 2 1 222 189 0.0 6 182 6 1 223 398 0.0 6 387 11 224 716 0.0 6 692 23 0 0 1 225 447 0.0 6 430 16 1 226 409 0.0 6 390 14 3 0 2 227 1417 0.0 6 1375 40 1 1 228 346 0.0 6 336 10 229 722 0.0 6 705 17 230 119 0.0 6 115 4 231 216 0.0 6 208 8 232 234 0.0 6 223 10 1 233 355 0.0 6 339 15 1 234 228 0.0 6 221 7 235 131 0.0 6 128 3 236 68 0.0 6 65 3 237 94 0.0 6 88 6 238 144 0.0 6 141 3 239 43 0.0 6 42 1 240 21 0.0 6 21 241 18 0.0 6 16 2 242 203 0.0 6 201 1 1 243 120 0.0 6 118 2 244 110 0.0 6 105 5 245 54 0.0 6 51 3 246 81 0.0 6 77 4 247 55 0.0 6 53 2 248 126 0.0 6 122 4 249 191 0.0 6 186 5 250 26 0.0 6 25 1 251 89 0.0 6 84 5 252 187 0.0 6 186 1 253 23 0.0 6 22 1 254 17 0.0 6 17 255 2 0.0 6 2 256 4 0.0 6 4 259 1 0.0 6 1 263 1 0.0 6 1 267 1 0.0 6 1 275 1 0.0 6 1 278 3 0.0 6 3 279 9 0.0 6 9 280 59 0.0 6 56 2 0 0 0 0 1 281 5 0.0 6 5 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2450 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 6 102 29.6 1 0 0 102 48 2 0.0 6 0 1 1 51 1 0.0 6 1 52 1 0.0 6 1 54 2 0.0 6 2 56 2 0.0 6 2 57 5 0.0 6 5 65 1 0.0 6 1 66 1 0.0 6 0 0 1 69 2 0.0 6 2 70 3 0.0 6 3 71 14 0.0 6 14 72 26 0.0 6 26 73 68 0.0 6 68 74 24 0.0 6 24 75 22 0.0 6 22 76 36 0.0 6 34 0 0 1 1 77 28 0.0 6 27 0 0 1 78 24 0.0 6 24 79 30 0.0 6 30 80 24 0.0 6 23 1 81 23 0.0 6 23 82 83 0.0 6 83 83 7 0.0 6 7 84 19 0.0 6 18 1 85 25 0.0 6 24 0 0 1 86 11 0.0 6 11 87 12 0.0 6 12 88 23 0.0 6 23 89 17 0.0 6 16 1 90 20 0.0 6 20 91 21 0.0 6 21 92 22 0.0 6 22 93 12 0.0 6 12 94 20 0.0 6 20 95 13 0.0 6 13 96 18 0.0 6 18 97 21 0.0 6 21 98 13 0.0 6 12 1 99 24 0.0 6 24 100 33 0.0 6 33 101 17 0.0 6 17 102 16 0.0 6 16 103 16 0.0 6 16 104 18 0.0 6 18 105 20 0.0 6 20 106 131 0.0 6 131 107 23 0.0 6 23 108 18 0.0 6 18 109 26 0.0 6 26 110 27 0.0 6 24 1 0 1 1 111 20 0.0 6 20 112 17 0.0 6 16 0 0 0 1 113 32 0.0 6 31 0 0 0 1 114 15 0.0 6 15 115 12 0.0 6 12 116 22 0.0 6 21 1 117 16 0.0 6 16 118 18 0.0 6 18 119 22 0.0 6 21 1 120 14 0.0 6 13 0 0 0 1 121 23 0.0 6 23 122 44 0.0 6 44 123 21 0.0 6 21 124 19 0.0 6 19 125 17 0.0 6 17 126 20 0.0 6 20 127 26 0.0 6 26 128 23 0.0 6 23 129 13 0.0 6 13 130 25 0.0 6 25 131 15 0.0 6 15 132 18 0.0 6 18 133 19 0.0 6 19 134 19 0.0 6 19 135 23 0.0 6 23 136 23 0.0 6 23 137 22 0.0 6 22 138 12 0.0 6 12 139 17 0.0 6 17 140 26 0.0 6 26 141 11 0.0 6 11 142 27 0.0 6 27 143 9 0.0 6 9 144 21 0.0 6 21 145 20 0.0 6 20 146 15 0.0 6 15 147 9 0.0 6 9 148 24 0.0 6 24 149 24 0.0 6 24 150 14 0.0 6 14 151 27 0.0 6 27 152 12 0.0 6 11 1 153 11 0.0 6 11 154 11 0.0 6 11 155 13 0.0 6 13 156 8 0.0 6 8 157 14 0.0 6 14 158 17 0.0 6 17 159 14 0.0 6 14 160 13 0.0 6 13 161 11 0.0 6 11 162 17 0.0 6 17 163 12 0.0 6 12 164 10 0.0 6 10 165 11 0.0 6 11 166 20 0.0 6 20 167 20 0.0 6 20 168 7 0.0 6 7 169 12 0.0 6 12 170 13 0.0 6 13 171 7 0.0 6 7 172 14 0.0 6 13 0 0 0 1 173 18 0.0 6 18 174 9 0.0 6 9 175 7 0.0 6 7 176 6 0.0 6 6 177 6 0.0 6 5 0 0 0 1 178 8 0.0 6 8 179 8 0.0 6 8 180 9 0.0 6 9 181 8 0.0 6 8 182 4 0.0 6 4 183 6 0.0 6 6 184 13 0.0 6 13 185 7 0.0 6 7 186 6 0.0 6 6 187 4 0.0 6 4 188 7 0.0 6 7 189 6 0.0 6 6 190 8 0.0 6 8 191 4 0.0 6 4 192 3 0.0 6 3 193 5 0.0 6 5 194 5 0.0 6 5 195 2 0.0 6 2 196 7 0.0 6 6 1 197 3 0.0 6 3 198 3 0.0 6 3 199 3 0.0 6 3 200 3 0.0 6 3 201 1 0.0 6 1 202 3 0.0 6 3 203 1 0.0 6 1 206 2 0.0 6 2 207 1 0.0 6 1 208 2 0.0 6 2 209 1 0.0 6 1 211 1 0.0 6 1 212 2 0.0 6 2 213 1 0.0 6 1 214 2 0.0 6 2 215 2 0.0 6 2 216 1 0.0 6 1 222 1 0.0 6 1 223 3 0.0 6 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_09_187_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_09_414_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_09_187_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_09_414_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.55 s (32 µs/read; 1.90 M reads/minute). === Summary === Total read pairs processed: 80,958 Read 1 with adapter: 80,958 (100.0%) Read 2 with adapter: 80,920 (100.0%) == Read fate breakdown == Pairs that were too short: 37 (0.0%) Pairs discarded as untrimmed: 38 (0.0%) Pairs written (passing filters): 80,883 (99.9%) Total basepairs processed: 33,888,293 bp Read 1: 18,952,915 bp Read 2: 14,935,378 bp Total written (filtered): 5,204,534 bp (15.4%) Read 1: 2,778,175 bp Read 2: 2,426,359 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 78687 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 4 316.2 1 4 5 1 79.1 1 1 6 1 19.8 1 1 7 4 4.9 2 3 0 1 9 2 0.3 2 2 13 1 0.0 3 1 14 1 0.0 4 1 15 2 0.0 4 1 1 16 1 0.0 4 0 1 17 1 0.0 5 0 0 1 18 2 0.0 5 2 19 3 0.0 5 2 1 20 86 0.0 6 83 3 21 33 0.0 6 31 2 22 44 0.0 6 43 1 23 28 0.0 6 27 1 24 19 0.0 6 18 1 25 23 0.0 6 22 1 26 13 0.0 6 13 27 19 0.0 6 19 28 5 0.0 6 5 29 8 0.0 6 8 30 6 0.0 6 6 31 11 0.0 6 11 32 10 0.0 6 10 33 6 0.0 6 6 34 6 0.0 6 6 35 4 0.0 6 4 36 5 0.0 6 5 37 7 0.0 6 7 38 6 0.0 6 6 39 12 0.0 6 12 40 5 0.0 6 5 41 6 0.0 6 6 42 7 0.0 6 7 43 2 0.0 6 2 44 2 0.0 6 2 45 7 0.0 6 7 46 8 0.0 6 7 1 47 6 0.0 6 6 48 7 0.0 6 6 1 49 7 0.0 6 7 50 7 0.0 6 7 51 6 0.0 6 6 52 10 0.0 6 10 53 7 0.0 6 6 1 54 5 0.0 6 4 1 55 6 0.0 6 6 56 4 0.0 6 2 2 57 3 0.0 6 3 58 8 0.0 6 8 59 5 0.0 6 5 60 5 0.0 6 5 61 4 0.0 6 4 62 4 0.0 6 4 63 11 0.0 6 11 64 7 0.0 6 7 65 15 0.0 6 15 66 10 0.0 6 9 1 67 6 0.0 6 6 68 5 0.0 6 5 69 9 0.0 6 8 1 70 8 0.0 6 7 1 71 11 0.0 6 10 1 72 13 0.0 6 13 73 13 0.0 6 13 74 20 0.0 6 19 1 75 5 0.0 6 5 76 11 0.0 6 10 1 77 2 0.0 6 2 78 6 0.0 6 6 79 7 0.0 6 7 80 9 0.0 6 9 81 7 0.0 6 7 82 10 0.0 6 10 83 17 0.0 6 17 84 19 0.0 6 17 0 0 2 85 13 0.0 6 13 86 10 0.0 6 10 87 11 0.0 6 11 88 10 0.0 6 10 89 5 0.0 6 5 90 4 0.0 6 4 91 15 0.0 6 15 92 5 0.0 6 5 93 14 0.0 6 14 94 16 0.0 6 16 95 13 0.0 6 13 96 8 0.0 6 8 97 15 0.0 6 15 98 7 0.0 6 7 99 23 0.0 6 23 100 14 0.0 6 13 1 101 20 0.0 6 20 102 22 0.0 6 21 1 103 18 0.0 6 18 104 29 0.0 6 28 1 105 25 0.0 6 24 1 106 17 0.0 6 17 107 21 0.0 6 20 1 108 26 0.0 6 26 109 33 0.0 6 33 110 25 0.0 6 25 111 30 0.0 6 30 112 26 0.0 6 26 113 20 0.0 6 19 1 114 33 0.0 6 32 0 0 0 0 1 115 24 0.0 6 24 116 36 0.0 6 35 0 0 0 0 1 117 48 0.0 6 28 0 0 0 0 20 118 43 0.0 6 39 1 0 0 0 3 119 26 0.0 6 25 0 0 0 0 1 120 24 0.0 6 24 121 31 0.0 6 31 122 38 0.0 6 38 123 24 0.0 6 24 124 28 0.0 6 28 125 39 0.0 6 38 0 0 1 126 35 0.0 6 34 0 0 0 0 0 1 127 51 0.0 6 48 2 0 0 0 1 128 51 0.0 6 51 129 41 0.0 6 39 2 130 47 0.0 6 47 131 70 0.0 6 69 1 132 47 0.0 6 47 133 47 0.0 6 47 134 42 0.0 6 42 135 58 0.0 6 56 2 136 56 0.0 6 56 137 69 0.0 6 67 2 138 72 0.0 6 70 2 139 109 0.0 6 107 2 140 212 0.0 6 211 1 141 476 0.0 6 469 4 2 0 1 142 609 0.0 6 609 143 523 0.0 6 522 1 144 431 0.0 6 428 3 145 741 0.0 6 741 146 689 0.0 6 689 147 691 0.0 6 689 1 0 0 0 1 148 736 0.0 6 736 149 684 0.0 6 681 2 0 0 0 0 1 150 1251 0.0 6 1249 2 151 621 0.0 6 618 2 0 0 0 1 152 530 0.0 6 528 1 0 1 153 441 0.0 6 440 1 154 431 0.0 6 431 155 338 0.0 6 338 156 321 0.0 6 319 1 0 0 0 0 1 157 457 0.0 6 457 158 502 0.0 6 500 2 159 586 0.0 6 585 1 160 445 0.0 6 442 3 161 399 0.0 6 396 1 0 1 0 0 1 162 445 0.0 6 445 163 431 0.0 6 430 1 164 401 0.0 6 400 0 1 165 321 0.0 6 318 3 166 356 0.0 6 356 167 447 0.0 6 446 1 168 368 0.0 6 365 2 0 1 169 399 0.0 6 398 1 170 375 0.0 6 373 2 171 436 0.0 6 434 1 0 0 0 0 1 172 457 0.0 6 456 1 173 424 0.0 6 424 174 441 0.0 6 441 175 423 0.0 6 422 1 176 327 0.0 6 326 1 177 349 0.0 6 346 2 0 0 0 1 178 982 0.0 6 980 2 179 503 0.0 6 501 2 180 418 0.0 6 413 4 1 181 584 0.0 6 581 3 182 470 0.0 6 468 2 183 426 0.0 6 426 184 463 0.0 6 460 2 0 0 0 0 1 185 522 0.0 6 522 186 498 0.0 6 498 187 449 0.0 6 443 6 188 488 0.0 6 486 2 189 471 0.0 6 470 1 190 580 0.0 6 575 4 0 1 191 645 0.0 6 642 3 192 649 0.0 6 648 0 0 0 0 1 193 654 0.0 6 648 4 0 0 2 194 666 0.0 6 661 5 195 810 0.0 6 807 3 196 934 0.0 6 929 3 1 0 0 1 197 992 0.0 6 982 9 1 198 800 0.0 6 793 6 1 199 756 0.0 6 755 1 200 786 0.0 6 780 6 201 653 0.0 6 649 4 202 1047 0.0 6 1041 5 0 0 0 1 203 671 0.0 6 665 4 1 0 0 1 204 827 0.0 6 826 1 205 1008 0.0 6 1002 6 206 893 0.0 6 890 3 207 1030 0.0 6 1024 6 208 757 0.0 6 752 4 0 0 0 1 209 843 0.0 6 840 3 210 852 0.0 6 844 7 0 0 0 1 211 853 0.0 6 848 4 1 212 905 0.0 6 900 5 213 916 0.0 6 910 5 0 0 0 1 214 1238 0.0 6 1230 8 215 868 0.0 6 861 7 216 939 0.0 6 936 2 1 217 975 0.0 6 971 2 2 218 891 0.0 6 888 1 1 0 1 219 774 0.0 6 773 1 220 765 0.0 6 759 5 0 0 0 0 1 221 777 0.0 6 774 3 222 741 0.0 6 735 5 0 1 223 861 0.0 6 859 2 224 856 0.0 6 852 4 225 926 0.0 6 918 7 0 0 0 1 226 738 0.0 6 736 2 227 643 0.0 6 641 2 228 633 0.0 6 629 3 0 1 229 717 0.0 6 712 5 230 679 0.0 6 677 2 231 688 0.0 6 684 2 1 0 0 1 232 722 0.0 6 719 2 0 0 1 233 700 0.0 6 697 3 234 528 0.0 6 527 0 1 235 609 0.0 6 606 1 1 0 0 0 1 236 559 0.0 6 555 4 237 587 0.0 6 584 1 0 0 0 2 238 611 0.0 6 607 3 1 239 545 0.0 6 544 1 240 510 0.0 6 509 1 241 561 0.0 6 556 3 1 0 1 242 518 0.0 6 518 243 453 0.0 6 453 244 386 0.0 6 383 3 245 315 0.0 6 312 2 0 0 0 0 1 246 328 0.0 6 326 2 247 349 0.0 6 347 1 1 248 333 0.0 6 333 249 356 0.0 6 354 2 250 330 0.0 6 327 3 251 287 0.0 6 284 3 252 294 0.0 6 292 2 253 281 0.0 6 277 3 0 0 0 0 1 254 237 0.0 6 235 1 0 0 1 255 242 0.0 6 239 3 256 246 0.0 6 244 2 257 238 0.0 6 236 2 258 276 0.0 6 274 1 1 259 222 0.0 6 220 2 260 153 0.0 6 152 1 261 184 0.0 6 184 262 181 0.0 6 181 263 182 0.0 6 180 2 264 120 0.0 6 120 265 176 0.0 6 175 1 266 177 0.0 6 176 1 267 141 0.0 6 139 2 268 187 0.0 6 186 1 269 143 0.0 6 143 270 125 0.0 6 125 271 108 0.0 6 108 272 155 0.0 6 154 1 273 175 0.0 6 173 2 274 192 0.0 6 191 1 275 234 0.0 6 234 276 340 0.0 6 338 2 277 982 0.0 6 966 13 1 1 0 1 278 1645 0.0 6 1636 6 1 1 0 1 279 27 0.0 6 26 1 280 2 0.0 6 2 282 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2271 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 11 316.2 1 11 61 1 0.0 6 0 0 0 1 63 62 0.0 6 9 49 4 64 189 0.0 6 183 6 65 1 0.0 6 1 66 45 0.0 6 44 1 70 1 0.0 6 1 72 1 0.0 6 1 73 11 0.0 6 11 74 15 0.0 6 15 75 18 0.0 6 18 76 17 0.0 6 17 77 14 0.0 6 14 78 24 0.0 6 24 79 22 0.0 6 22 80 19 0.0 6 19 81 23 0.0 6 23 82 31 0.0 6 31 83 59 0.0 6 59 84 17 0.0 6 17 85 19 0.0 6 19 86 11 0.0 6 11 87 10 0.0 6 10 88 7 0.0 6 7 89 9 0.0 6 9 90 15 0.0 6 15 91 10 0.0 6 10 92 16 0.0 6 16 93 10 0.0 6 10 94 10 0.0 6 10 95 7 0.0 6 7 96 11 0.0 6 11 97 10 0.0 6 10 98 7 0.0 6 7 99 10 0.0 6 10 100 12 0.0 6 12 101 5 0.0 6 5 102 14 0.0 6 14 103 10 0.0 6 10 104 21 0.0 6 21 105 9 0.0 6 9 106 8 0.0 6 8 107 6 0.0 6 6 108 8 0.0 6 8 109 5 0.0 6 5 110 15 0.0 6 15 111 35 0.0 6 35 112 12 0.0 6 12 113 11 0.0 6 11 114 14 0.0 6 14 115 8 0.0 6 7 0 0 0 0 0 1 116 10 0.0 6 10 117 5 0.0 6 5 118 12 0.0 6 12 119 8 0.0 6 8 120 12 0.0 6 12 121 5 0.0 6 5 122 4 0.0 6 4 123 12 0.0 6 12 124 11 0.0 6 11 125 14 0.0 6 14 126 5 0.0 6 5 127 5 0.0 6 5 128 11 0.0 6 11 129 7 0.0 6 7 130 7 0.0 6 7 131 10 0.0 6 10 132 9 0.0 6 9 133 13 0.0 6 13 134 8 0.0 6 8 135 20 0.0 6 20 136 13 0.0 6 13 137 9 0.0 6 9 138 5 0.0 6 5 139 9 0.0 6 9 140 12 0.0 6 12 141 14 0.0 6 14 142 15 0.0 6 15 143 11 0.0 6 11 144 8 0.0 6 8 145 10 0.0 6 10 146 6 0.0 6 6 147 8 0.0 6 8 148 8 0.0 6 8 149 9 0.0 6 9 150 16 0.0 6 15 0 0 0 0 0 1 151 11 0.0 6 11 152 24 0.0 6 24 153 10 0.0 6 10 154 6 0.0 6 6 155 13 0.0 6 13 156 7 0.0 6 7 157 8 0.0 6 8 158 15 0.0 6 15 159 11 0.0 6 11 160 8 0.0 6 8 161 9 0.0 6 9 162 9 0.0 6 9 163 16 0.0 6 16 164 17 0.0 6 17 165 19 0.0 6 19 166 19 0.0 6 19 167 12 0.0 6 12 168 8 0.0 6 8 169 9 0.0 6 9 170 13 0.0 6 13 171 12 0.0 6 12 172 11 0.0 6 11 173 18 0.0 6 18 174 15 0.0 6 15 175 10 0.0 6 10 176 11 0.0 6 11 177 5 0.0 6 5 178 10 0.0 6 10 179 12 0.0 6 12 180 7 0.0 6 7 181 9 0.0 6 9 182 10 0.0 6 10 183 16 0.0 6 16 184 7 0.0 6 7 185 5 0.0 6 5 186 11 0.0 6 11 187 15 0.0 6 15 188 15 0.0 6 15 189 9 0.0 6 9 190 12 0.0 6 12 191 12 0.0 6 12 192 21 0.0 6 21 193 10 0.0 6 10 194 14 0.0 6 14 195 13 0.0 6 12 0 0 0 0 0 1 196 8 0.0 6 8 197 4 0.0 6 4 198 11 0.0 6 11 199 12 0.0 6 12 200 6 0.0 6 6 201 9 0.0 6 9 202 13 0.0 6 13 203 6 0.0 6 6 204 12 0.0 6 12 205 10 0.0 6 10 206 14 0.0 6 14 207 11 0.0 6 11 208 15 0.0 6 15 209 11 0.0 6 11 210 9 0.0 6 9 211 7 0.0 6 7 212 5 0.0 6 5 213 13 0.0 6 13 214 4 0.0 6 4 215 14 0.0 6 14 216 10 0.0 6 10 217 6 0.0 6 6 218 2 0.0 6 2 219 9 0.0 6 9 220 6 0.0 6 6 221 7 0.0 6 7 222 1 0.0 6 1 223 4 0.0 6 4 224 9 0.0 6 9 225 4 0.0 6 4 226 10 0.0 6 10 227 5 0.0 6 5 228 8 0.0 6 8 229 5 0.0 6 5 230 12 0.0 6 12 231 2 0.0 6 2 232 11 0.0 6 10 1 233 6 0.0 6 6 234 2 0.0 6 2 235 3 0.0 6 3 236 8 0.0 6 8 237 6 0.0 6 6 238 1 0.0 6 1 239 5 0.0 6 5 240 6 0.0 6 6 241 4 0.0 6 4 242 5 0.0 6 5 244 5 0.0 6 5 245 1 0.0 6 1 246 5 0.0 6 5 247 4 0.0 6 4 248 2 0.0 6 2 249 4 0.0 6 4 250 6 0.0 6 6 251 2 0.0 6 2 252 6 0.0 6 6 253 4 0.0 6 4 254 2 0.0 6 2 255 6 0.0 6 6 256 1 0.0 6 1 257 3 0.0 6 3 258 3 0.0 6 3 259 5 0.0 6 5 260 2 0.0 6 2 261 1 0.0 6 1 262 1 0.0 6 1 263 2 0.0 6 2 264 3 0.0 6 3 265 3 0.0 6 2 1 266 1 0.0 6 1 267 2 0.0 6 2 268 2 0.0 6 2 269 2 0.0 6 2 270 2 0.0 6 2 271 2 0.0 6 2 272 2 0.0 6 2 273 1 0.0 6 1 274 4 0.0 6 4 275 3 0.0 6 3 276 1 0.0 6 1 277 1 0.0 6 1 280 1 0.0 6 1 281 2 0.0 6 2 282 1 0.0 6 1 283 2 0.0 6 2 289 1 0.0 6 1 292 1 0.0 6 1 293 1 0.0 6 1 295 1 0.0 6 1 296 1 0.0 6 1 298 2 0.0 6 2 300 1 0.0 6 1 311 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 78823 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1265.0 0 1 10 1 0.1 3 1 11 1 0.0 3 1 12 5 0.0 3 5 13 6 0.0 3 5 1 14 6 0.0 4 4 1 0 1 15 3 0.0 4 3 16 4 0.0 4 1 3 17 24 0.0 5 24 18 4 0.0 5 3 1 19 55 0.0 5 50 4 1 20 102 0.0 6 99 2 0 0 1 21 46 0.0 6 45 1 22 46 0.0 6 43 3 23 28 0.0 6 24 4 24 20 0.0 6 18 2 25 22 0.0 6 19 3 26 17 0.0 6 15 1 0 0 0 1 27 19 0.0 6 16 2 1 28 23 0.0 6 22 1 29 13 0.0 6 12 1 30 16 0.0 6 14 1 1 31 12 0.0 6 12 32 14 0.0 6 13 1 33 16 0.0 6 15 1 34 19 0.0 6 18 1 35 13 0.0 6 10 2 1 36 17 0.0 6 17 37 20 0.0 6 16 2 1 0 0 1 38 13 0.0 6 13 39 11 0.0 6 10 0 0 1 40 21 0.0 6 18 2 0 0 1 41 20 0.0 6 18 1 1 42 11 0.0 6 9 2 43 17 0.0 6 14 3 44 15 0.0 6 12 3 45 13 0.0 6 12 0 0 0 0 0 1 46 25 0.0 6 23 0 1 0 0 0 1 47 12 0.0 6 10 1 0 1 48 13 0.0 6 12 1 49 25 0.0 6 25 50 22 0.0 6 21 0 1 51 29 0.0 6 26 2 1 52 24 0.0 6 21 2 0 0 1 53 25 0.0 6 21 3 1 54 25 0.0 6 24 1 55 22 0.0 6 21 0 1 56 25 0.0 6 23 2 57 24 0.0 6 21 2 1 58 26 0.0 6 23 3 59 28 0.0 6 25 1 1 1 60 36 0.0 6 31 4 0 0 0 1 61 35 0.0 6 33 1 0 1 62 32 0.0 6 29 3 63 31 0.0 6 29 2 64 25 0.0 6 23 2 65 33 0.0 6 32 1 66 56 0.0 6 54 2 67 35 0.0 6 32 2 0 0 1 68 28 0.0 6 26 2 69 57 0.0 6 52 3 1 0 1 70 180 0.0 6 174 5 1 71 66 0.0 6 59 5 0 0 0 0 2 72 103 0.0 6 100 3 73 45 0.0 6 43 1 0 0 1 74 63 0.0 6 60 2 1 75 44 0.0 6 44 76 46 0.0 6 43 2 0 1 77 64 0.0 6 60 3 0 1 78 45 0.0 6 43 2 79 45 0.0 6 43 2 80 55 0.0 6 50 5 81 52 0.0 6 48 4 82 62 0.0 6 59 3 83 96 0.0 6 91 4 1 84 69 0.0 6 64 1 2 2 85 80 0.0 6 77 1 1 1 86 89 0.0 6 82 3 1 1 2 87 58 0.0 6 55 2 0 0 0 1 88 54 0.0 6 50 4 89 48 0.0 6 45 2 0 0 0 1 90 72 0.0 6 70 1 1 91 65 0.0 6 65 92 76 0.0 6 74 2 93 99 0.0 6 92 5 1 0 0 0 1 94 71 0.0 6 68 3 95 94 0.0 6 91 3 96 87 0.0 6 84 3 97 114 0.0 6 111 2 0 0 1 98 115 0.0 6 111 4 99 120 0.0 6 117 3 100 113 0.0 6 104 7 0 0 2 101 118 0.0 6 113 2 1 0 0 1 1 102 98 0.0 6 97 1 103 133 0.0 6 131 2 104 120 0.0 6 115 3 1 1 105 114 0.0 6 110 4 106 151 0.0 6 145 4 1 1 107 229 0.0 6 222 5 1 1 108 144 0.0 6 140 2 0 1 0 1 109 116 0.0 6 112 4 110 145 0.0 6 138 7 111 159 0.0 6 151 7 1 112 160 0.0 6 156 3 1 113 176 0.0 6 171 5 114 189 0.0 6 179 9 0 0 0 0 1 115 212 0.0 6 204 4 2 1 0 0 1 116 169 0.0 6 165 3 1 117 208 0.0 6 199 7 1 1 118 224 0.0 6 222 2 119 195 0.0 6 189 6 120 283 0.0 6 272 9 0 0 1 1 121 247 0.0 6 242 4 0 0 0 1 122 241 0.0 6 240 1 123 221 0.0 6 218 3 124 242 0.0 6 241 1 125 279 0.0 6 270 4 1 1 0 1 2 126 292 0.0 6 279 10 0 1 0 1 1 127 362 0.0 6 352 8 1 0 0 0 1 128 456 0.0 6 444 11 0 0 1 129 383 0.0 6 367 15 0 0 1 130 414 0.0 6 399 9 2 1 2 1 131 539 0.0 6 507 29 2 0 1 132 552 0.0 6 536 15 0 0 0 0 1 133 525 0.0 6 513 10 0 0 0 1 1 134 606 0.0 6 596 10 135 559 0.0 6 548 8 1 0 2 136 620 0.0 6 614 5 0 0 1 137 628 0.0 6 614 10 2 0 0 0 2 138 630 0.0 6 621 7 0 0 0 0 2 139 615 0.0 6 607 7 0 1 140 650 0.0 6 640 7 0 1 1 1 141 707 0.0 6 701 6 142 985 0.0 6 972 12 0 0 0 1 143 1081 0.0 6 1072 8 0 0 0 1 144 1100 0.0 6 1089 8 1 0 1 1 145 1164 0.0 6 1152 9 1 0 1 1 146 1271 0.0 6 1257 12 0 0 0 2 147 1319 0.0 6 1305 10 1 0 2 1 148 1421 0.0 6 1399 17 1 0 1 2 1 149 1528 0.0 6 1511 14 0 1 1 1 150 1474 0.0 6 1460 9 3 0 0 1 1 151 2010 0.0 6 1993 12 1 1 1 1 1 152 1742 0.0 6 1711 29 0 0 2 153 2014 0.0 6 1987 22 0 2 1 1 1 154 1605 0.0 6 1578 23 1 0 0 3 155 1785 0.0 6 1760 21 1 0 1 1 1 156 1684 0.0 6 1663 14 2 3 0 0 2 157 1435 0.0 6 1415 15 0 1 1 1 2 158 1527 0.0 6 1508 18 0 0 1 159 1378 0.0 6 1363 11 2 1 1 160 1523 0.0 6 1498 21 2 1 0 0 1 161 1394 0.0 6 1376 13 0 2 1 2 162 1264 0.0 6 1246 11 2 3 0 2 163 1272 0.0 6 1258 12 1 0 1 164 1151 0.0 6 1144 5 0 1 1 165 1009 0.0 6 990 18 0 1 166 1062 0.0 6 1045 17 167 1011 0.0 6 1001 9 0 0 0 1 168 930 0.0 6 918 10 1 0 0 1 169 1121 0.0 6 1108 10 0 1 0 2 170 1014 0.0 6 1006 5 1 0 0 1 1 171 1238 0.0 6 1223 12 1 1 0 1 172 992 0.0 6 984 6 1 0 1 173 993 0.0 6 981 10 0 0 1 1 174 1059 0.0 6 1050 8 0 0 0 1 175 1010 0.0 6 1000 9 0 0 0 1 176 1012 0.0 6 1003 6 0 1 1 0 1 177 914 0.0 6 905 5 1 1 1 0 1 178 934 0.0 6 923 9 0 1 0 1 179 1024 0.0 6 1016 6 0 2 180 732 0.0 6 720 10 1 0 0 0 1 181 745 0.0 6 737 8 182 659 0.0 6 654 2 1 0 1 0 1 183 550 0.0 6 546 3 0 0 0 1 184 581 0.0 6 575 5 1 185 538 0.0 6 531 7 186 732 0.0 6 728 4 187 901 0.0 6 895 5 0 0 1 188 779 0.0 6 770 7 0 1 1 189 565 0.0 6 554 11 190 492 0.0 6 488 3 1 191 497 0.0 6 493 4 192 461 0.0 6 454 5 1 0 1 193 418 0.0 6 415 3 194 395 0.0 6 392 3 195 317 0.0 6 314 3 196 304 0.0 6 301 3 197 267 0.0 6 264 2 1 198 226 0.0 6 225 1 199 334 0.0 6 332 1 1 200 235 0.0 6 235 201 236 0.0 6 235 1 202 188 0.0 6 188 203 190 0.0 6 188 2 204 133 0.0 6 133 205 152 0.0 6 152 206 139 0.0 6 138 0 1 207 114 0.0 6 111 2 0 0 0 0 1 208 123 0.0 6 122 1 209 121 0.0 6 119 2 210 89 0.0 6 88 1 211 105 0.0 6 104 0 1 212 95 0.0 6 92 2 1 213 60 0.0 6 59 1 214 59 0.0 6 59 215 75 0.0 6 75 216 84 0.0 6 83 1 217 81 0.0 6 79 2 218 61 0.0 6 61 219 54 0.0 6 53 1 220 63 0.0 6 62 1 221 54 0.0 6 54 222 47 0.0 6 47 223 42 0.0 6 42 224 56 0.0 6 55 1 225 36 0.0 6 36 226 23 0.0 6 23 227 26 0.0 6 26 228 24 0.0 6 23 1 229 16 0.0 6 16 230 13 0.0 6 13 231 10 0.0 6 10 232 18 0.0 6 18 233 11 0.0 6 11 234 8 0.0 6 8 235 9 0.0 6 9 236 7 0.0 6 7 237 2 0.0 6 2 238 3 0.0 6 3 239 2 0.0 6 2 240 4 0.0 6 4 241 5 0.0 6 5 242 8 0.0 6 7 1 243 4 0.0 6 4 244 4 0.0 6 4 245 2 0.0 6 2 246 3 0.0 6 3 249 2 0.0 6 2 256 1 0.0 6 1 280 1 0.0 6 1 281 15 0.0 6 15 282 79 0.0 6 79 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2097 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 24 0.0 6 0 24 49 10 0.0 6 10 50 2 0.0 6 1 0 0 0 0 1 56 25 0.0 6 24 1 59 11 0.0 6 11 60 24 0.0 6 24 65 1 0.0 6 0 1 66 16 0.0 6 15 1 68 7 0.0 6 7 69 7 0.0 6 7 70 17 0.0 6 17 71 17 0.0 6 17 72 26 0.0 6 25 1 73 23 0.0 6 23 74 20 0.0 6 20 75 28 0.0 6 28 76 32 0.0 6 31 0 0 0 1 77 25 0.0 6 25 78 52 0.0 6 52 79 89 0.0 6 89 80 22 0.0 6 22 81 18 0.0 6 18 82 25 0.0 6 24 0 0 1 83 13 0.0 6 13 84 13 0.0 6 13 85 17 0.0 6 17 86 20 0.0 6 20 87 25 0.0 6 25 88 128 0.0 6 127 1 89 28 0.0 6 28 90 14 0.0 6 14 91 11 0.0 6 11 92 17 0.0 6 17 93 14 0.0 6 14 94 12 0.0 6 11 1 95 10 0.0 6 10 96 13 0.0 6 13 97 9 0.0 6 9 98 11 0.0 6 11 99 13 0.0 6 13 100 12 0.0 6 12 101 34 0.0 6 34 102 16 0.0 6 16 103 8 0.0 6 8 104 10 0.0 6 10 105 4 0.0 6 4 106 7 0.0 6 7 107 28 0.0 6 28 108 24 0.0 6 24 109 13 0.0 6 13 110 20 0.0 6 20 111 4 0.0 6 4 112 13 0.0 6 12 0 0 1 113 10 0.0 6 10 114 14 0.0 6 14 115 16 0.0 6 16 116 6 0.0 6 6 117 11 0.0 6 10 0 0 0 1 118 4 0.0 6 4 119 6 0.0 6 6 120 14 0.0 6 14 121 13 0.0 6 13 122 7 0.0 6 7 123 4 0.0 6 4 124 7 0.0 6 7 125 14 0.0 6 14 126 8 0.0 6 8 127 10 0.0 6 10 128 13 0.0 6 13 129 20 0.0 6 20 130 12 0.0 6 12 131 15 0.0 6 15 132 7 0.0 6 7 133 12 0.0 6 11 0 0 0 1 134 12 0.0 6 12 135 16 0.0 6 16 136 27 0.0 6 27 137 11 0.0 6 11 138 26 0.0 6 26 139 7 0.0 6 7 140 12 0.0 6 12 141 7 0.0 6 7 142 9 0.0 6 8 0 0 0 0 0 1 143 18 0.0 6 17 0 0 0 1 144 5 0.0 6 5 145 11 0.0 6 11 146 20 0.0 6 20 147 3 0.0 6 3 148 14 0.0 6 14 149 15 0.0 6 15 150 13 0.0 6 13 151 13 0.0 6 13 152 13 0.0 6 13 153 12 0.0 6 11 0 0 1 154 18 0.0 6 18 155 16 0.0 6 15 0 0 0 1 156 10 0.0 6 10 157 10 0.0 6 10 158 12 0.0 6 11 0 0 0 1 159 9 0.0 6 9 160 17 0.0 6 17 161 17 0.0 6 17 162 17 0.0 6 17 163 12 0.0 6 12 164 8 0.0 6 8 165 12 0.0 6 12 166 19 0.0 6 19 167 13 0.0 6 13 168 14 0.0 6 14 169 14 0.0 6 14 170 17 0.0 6 17 171 26 0.0 6 26 172 10 0.0 6 10 173 12 0.0 6 12 174 9 0.0 6 9 175 9 0.0 6 9 176 14 0.0 6 13 1 177 9 0.0 6 9 178 12 0.0 6 12 179 10 0.0 6 10 180 9 0.0 6 9 181 6 0.0 6 6 182 10 0.0 6 10 183 8 0.0 6 8 184 9 0.0 6 9 185 16 0.0 6 16 186 7 0.0 6 6 1 187 9 0.0 6 9 188 10 0.0 6 10 189 5 0.0 6 5 190 1 0.0 6 1 191 4 0.0 6 4 192 4 0.0 6 4 193 2 0.0 6 2 194 1 0.0 6 1 195 5 0.0 6 5 196 4 0.0 6 4 197 3 0.0 6 3 198 6 0.0 6 6 199 3 0.0 6 3 200 3 0.0 6 3 201 1 0.0 6 1 202 3 0.0 6 2 0 1 203 5 0.0 6 5 204 8 0.0 6 8 205 4 0.0 6 4 206 1 0.0 6 1 207 4 0.0 6 4 208 3 0.0 6 3 210 1 0.0 6 1 211 2 0.0 6 2 212 6 0.0 6 6 213 1 0.0 6 1 214 3 0.0 6 2 1 215 1 0.0 6 1 217 2 0.0 6 2 218 3 0.0 6 3 222 1 0.0 6 1 223 1 0.0 6 1 224 2 0.0 6 2 227 1 0.0 6 1 230 1 0.0 6 1 233 2 0.0 6 2 236 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_10_188_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_10_415_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_10_188_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_10_415_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.86 s (34 µs/read; 1.78 M reads/minute). === Summary === Total read pairs processed: 55,181 Read 1 with adapter: 55,181 (100.0%) Read 2 with adapter: 55,164 (100.0%) == Read fate breakdown == Pairs that were too short: 25 (0.0%) Pairs discarded as untrimmed: 17 (0.0%) Pairs written (passing filters): 55,139 (99.9%) Total basepairs processed: 22,479,468 bp Read 1: 12,346,855 bp Read 2: 10,132,613 bp Total written (filtered): 3,734,474 bp (16.6%) Read 1: 1,905,409 bp Read 2: 1,829,065 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 53575 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 215.6 1 1 5 3 53.9 1 3 6 3 13.5 1 2 0 1 7 2 3.4 2 1 0 1 8 2 0.8 2 2 10 1 0.1 3 1 17 2 0.0 5 1 1 18 3 0.0 5 3 19 3 0.0 5 3 20 46 0.0 6 44 2 21 33 0.0 6 32 1 22 30 0.0 6 29 1 23 22 0.0 6 21 1 24 22 0.0 6 21 1 25 12 0.0 6 11 0 1 26 8 0.0 6 6 2 27 9 0.0 6 8 1 28 8 0.0 6 8 29 2 0.0 6 2 30 12 0.0 6 12 31 2 0.0 6 2 32 2 0.0 6 2 33 3 0.0 6 3 34 6 0.0 6 6 35 5 0.0 6 4 0 0 0 0 0 1 36 6 0.0 6 5 1 37 4 0.0 6 4 38 4 0.0 6 4 39 5 0.0 6 4 1 40 3 0.0 6 3 41 4 0.0 6 4 42 5 0.0 6 5 43 7 0.0 6 7 44 5 0.0 6 4 0 0 0 0 1 45 1 0.0 6 0 1 46 3 0.0 6 3 47 3 0.0 6 2 1 48 5 0.0 6 4 1 49 5 0.0 6 4 1 50 3 0.0 6 3 51 3 0.0 6 3 52 2 0.0 6 2 53 5 0.0 6 5 54 4 0.0 6 3 1 55 3 0.0 6 3 56 3 0.0 6 3 57 3 0.0 6 3 58 6 0.0 6 5 1 59 3 0.0 6 3 60 2 0.0 6 2 62 2 0.0 6 2 63 6 0.0 6 6 64 1 0.0 6 1 65 9 0.0 6 9 66 4 0.0 6 4 67 6 0.0 6 5 0 0 1 68 6 0.0 6 6 69 9 0.0 6 9 70 11 0.0 6 11 71 9 0.0 6 8 1 72 10 0.0 6 10 73 11 0.0 6 11 74 11 0.0 6 11 75 10 0.0 6 10 76 7 0.0 6 7 77 4 0.0 6 4 78 7 0.0 6 7 79 8 0.0 6 8 80 6 0.0 6 5 1 81 11 0.0 6 11 82 12 0.0 6 12 83 12 0.0 6 12 84 11 0.0 6 11 85 5 0.0 6 4 0 0 0 1 86 14 0.0 6 12 2 87 5 0.0 6 5 88 17 0.0 6 16 1 89 5 0.0 6 5 90 4 0.0 6 4 91 14 0.0 6 14 92 7 0.0 6 6 1 93 11 0.0 6 10 1 94 12 0.0 6 12 95 9 0.0 6 8 1 96 4 0.0 6 4 97 9 0.0 6 9 98 11 0.0 6 9 2 99 15 0.0 6 15 100 13 0.0 6 13 101 8 0.0 6 7 1 102 12 0.0 6 12 103 13 0.0 6 13 104 18 0.0 6 18 105 12 0.0 6 11 1 106 11 0.0 6 11 107 11 0.0 6 11 108 13 0.0 6 13 109 19 0.0 6 19 110 21 0.0 6 21 111 24 0.0 6 23 1 112 18 0.0 6 18 113 36 0.0 6 36 114 22 0.0 6 22 115 31 0.0 6 30 1 116 28 0.0 6 26 1 0 0 0 0 1 117 20 0.0 6 20 118 22 0.0 6 22 119 22 0.0 6 22 120 28 0.0 6 28 121 42 0.0 6 42 122 37 0.0 6 37 123 37 0.0 6 37 124 29 0.0 6 29 125 28 0.0 6 28 126 31 0.0 6 30 1 127 43 0.0 6 42 1 128 49 0.0 6 49 129 57 0.0 6 57 130 35 0.0 6 35 131 48 0.0 6 48 132 54 0.0 6 54 133 51 0.0 6 50 1 134 60 0.0 6 60 135 35 0.0 6 33 1 1 136 39 0.0 6 38 0 0 1 137 39 0.0 6 39 138 38 0.0 6 38 139 77 0.0 6 76 1 140 48 0.0 6 48 141 90 0.0 6 89 1 142 102 0.0 6 102 143 226 0.0 6 225 1 144 515 0.0 6 515 145 340 0.0 6 340 146 437 0.0 6 436 1 147 728 0.0 6 724 4 148 616 0.0 6 615 1 149 887 0.0 6 886 1 150 555 0.0 6 554 1 151 486 0.0 6 486 152 538 0.0 6 538 153 898 0.0 6 896 2 154 1019 0.0 6 1013 6 155 960 0.0 6 959 1 156 659 0.0 6 658 0 0 0 1 157 204 0.0 6 203 1 158 195 0.0 6 195 159 500 0.0 6 498 1 1 160 667 0.0 6 667 161 737 0.0 6 735 1 0 0 0 0 1 162 1458 0.0 6 1456 2 163 851 0.0 6 850 1 164 717 0.0 6 713 4 165 1078 0.0 6 1075 2 0 0 0 0 1 166 561 0.0 6 560 1 167 974 0.0 6 972 1 0 0 1 168 381 0.0 6 381 169 402 0.0 6 401 1 170 272 0.0 6 270 1 0 0 0 0 1 171 595 0.0 6 593 2 172 712 0.0 6 709 3 173 282 0.0 6 281 1 174 507 0.0 6 503 2 1 0 1 175 479 0.0 6 477 2 176 180 0.0 6 179 0 1 177 170 0.0 6 169 0 0 1 178 299 0.0 6 298 0 0 0 0 1 179 466 0.0 6 462 4 180 385 0.0 6 383 2 181 362 0.0 6 361 1 182 195 0.0 6 195 183 179 0.0 6 177 1 0 1 184 230 0.0 6 229 1 185 254 0.0 6 252 2 186 760 0.0 6 759 1 187 177 0.0 6 176 1 188 155 0.0 6 154 1 189 294 0.0 6 292 1 0 1 190 883 0.0 6 882 1 191 272 0.0 6 272 192 200 0.0 6 199 1 193 220 0.0 6 217 1 0 2 194 443 0.0 6 437 5 1 195 474 0.0 6 469 5 196 503 0.0 6 503 197 357 0.0 6 353 4 198 363 0.0 6 359 2 0 0 0 0 2 199 232 0.0 6 228 4 200 332 0.0 6 332 201 422 0.0 6 419 3 202 477 0.0 6 474 3 203 934 0.0 6 928 6 204 376 0.0 6 372 3 0 0 0 0 1 205 506 0.0 6 502 3 0 0 0 0 1 206 432 0.0 6 426 5 0 0 0 1 207 387 0.0 6 384 3 208 373 0.0 6 370 3 209 302 0.0 6 299 3 210 448 0.0 6 437 8 1 0 1 1 211 520 0.0 6 514 4 1 0 1 212 714 0.0 6 711 3 213 723 0.0 6 719 3 1 214 719 0.0 6 713 6 215 503 0.0 6 497 6 216 552 0.0 6 548 2 1 0 0 1 217 611 0.0 6 607 4 218 416 0.0 6 416 219 299 0.0 6 296 2 1 220 249 0.0 6 247 1 0 1 221 272 0.0 6 271 1 222 325 0.0 6 323 2 223 404 0.0 6 401 2 0 0 0 0 1 224 555 0.0 6 550 4 0 0 1 225 416 0.0 6 412 3 1 226 382 0.0 6 380 2 227 358 0.0 6 356 2 228 358 0.0 6 354 3 0 1 229 383 0.0 6 381 1 1 230 275 0.0 6 269 5 1 231 266 0.0 6 264 2 232 311 0.0 6 308 3 233 375 0.0 6 374 1 234 329 0.0 6 326 2 0 1 235 291 0.0 6 289 2 236 235 0.0 6 233 1 0 0 0 1 237 209 0.0 6 206 2 0 0 1 238 256 0.0 6 255 1 239 259 0.0 6 258 1 240 286 0.0 6 283 3 241 334 0.0 6 332 2 242 310 0.0 6 308 2 243 240 0.0 6 238 2 244 257 0.0 6 257 245 353 0.0 6 349 2 1 0 0 0 1 246 283 0.0 6 283 247 225 0.0 6 225 248 134 0.0 6 133 1 249 157 0.0 6 155 2 250 177 0.0 6 176 1 251 147 0.0 6 146 1 252 145 0.0 6 145 253 182 0.0 6 180 1 0 0 0 1 254 131 0.0 6 131 255 141 0.0 6 140 1 256 133 0.0 6 131 1 0 0 0 0 1 257 153 0.0 6 151 1 0 0 0 1 258 153 0.0 6 152 1 259 139 0.0 6 138 1 260 69 0.0 6 68 1 261 55 0.0 6 52 3 262 89 0.0 6 87 2 263 75 0.0 6 74 1 264 85 0.0 6 84 1 265 111 0.0 6 111 266 58 0.0 6 58 267 67 0.0 6 66 1 268 65 0.0 6 65 269 46 0.0 6 44 2 270 44 0.0 6 44 271 43 0.0 6 42 1 272 48 0.0 6 48 273 71 0.0 6 69 1 1 274 66 0.0 6 63 3 275 102 0.0 6 102 276 119 0.0 6 118 1 277 436 0.0 6 431 3 2 278 648 0.0 6 638 9 0 0 0 0 1 279 20 0.0 6 20 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1606 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 7 215.6 1 7 66 1 0.0 6 1 72 3 0.0 6 3 73 14 0.0 6 14 74 14 0.0 6 14 75 16 0.0 6 16 76 12 0.0 6 12 77 22 0.0 6 21 1 78 24 0.0 6 24 79 32 0.0 6 32 80 15 0.0 6 15 81 22 0.0 6 22 82 24 0.0 6 24 83 41 0.0 6 41 84 43 0.0 6 43 85 30 0.0 6 30 86 16 0.0 6 16 87 2 0.0 6 2 88 9 0.0 6 9 89 23 0.0 6 23 90 21 0.0 6 21 91 34 0.0 6 34 92 46 0.0 6 45 1 93 17 0.0 6 17 94 31 0.0 6 31 95 43 0.0 6 43 96 24 0.0 6 24 97 25 0.0 6 25 98 13 0.0 6 13 99 16 0.0 6 16 100 13 0.0 6 13 101 25 0.0 6 25 102 27 0.0 6 27 103 9 0.0 6 9 104 17 0.0 6 17 105 12 0.0 6 12 106 3 0.0 6 3 107 3 0.0 6 3 108 12 0.0 6 12 109 12 0.0 6 12 110 13 0.0 6 13 111 13 0.0 6 13 112 1 0.0 6 1 113 1 0.0 6 1 114 3 0.0 6 3 115 11 0.0 6 11 116 28 0.0 6 28 117 3 0.0 6 3 118 1 0.0 6 1 119 10 0.0 6 10 120 42 0.0 6 42 121 2 0.0 6 2 123 3 0.0 6 3 124 8 0.0 6 8 125 8 0.0 6 8 126 3 0.0 6 3 127 1 0.0 6 1 128 2 0.0 6 2 129 3 0.0 6 3 131 5 0.0 6 5 132 15 0.0 6 15 133 45 0.0 6 45 134 6 0.0 6 6 135 6 0.0 6 6 136 2 0.0 6 2 137 5 0.0 6 5 138 4 0.0 6 4 139 2 0.0 6 2 140 6 0.0 6 6 141 2 0.0 6 2 142 2 0.0 6 2 143 10 0.0 6 10 144 4 0.0 6 4 145 9 0.0 6 9 146 8 0.0 6 8 147 14 0.0 6 14 148 7 0.0 6 7 149 3 0.0 6 3 151 1 0.0 6 0 0 0 0 0 0 1 152 3 0.0 6 3 154 8 0.0 6 8 155 3 0.0 6 3 157 2 0.0 6 2 158 10 0.0 6 10 159 12 0.0 6 12 160 1 0.0 6 1 162 5 0.0 6 5 163 8 0.0 6 8 164 24 0.0 6 24 165 10 0.0 6 10 166 3 0.0 6 3 167 2 0.0 6 2 168 1 0.0 6 1 169 12 0.0 6 12 170 1 0.0 6 1 171 2 0.0 6 2 173 1 0.0 6 0 1 174 16 0.0 6 16 175 46 0.0 6 46 176 27 0.0 6 27 177 31 0.0 6 31 178 15 0.0 6 15 179 2 0.0 6 2 180 8 0.0 6 8 181 3 0.0 6 3 182 2 0.0 6 2 184 2 0.0 6 2 185 1 0.0 6 1 186 7 0.0 6 7 187 20 0.0 6 20 188 7 0.0 6 7 189 9 0.0 6 9 190 7 0.0 6 7 191 2 0.0 6 2 192 4 0.0 6 4 193 6 0.0 6 5 0 0 0 0 0 1 194 1 0.0 6 1 195 2 0.0 6 2 196 5 0.0 6 5 198 3 0.0 6 3 199 2 0.0 6 2 200 2 0.0 6 2 202 1 0.0 6 1 203 4 0.0 6 4 204 2 0.0 6 2 205 10 0.0 6 10 206 8 0.0 6 8 207 3 0.0 6 3 208 1 0.0 6 1 210 1 0.0 6 1 211 1 0.0 6 1 212 5 0.0 6 5 213 5 0.0 6 5 214 1 0.0 6 1 215 4 0.0 6 4 216 4 0.0 6 4 217 1 0.0 6 1 218 3 0.0 6 3 219 7 0.0 6 7 220 4 0.0 6 4 221 6 0.0 6 6 222 12 0.0 6 11 1 223 2 0.0 6 2 225 6 0.0 6 6 226 4 0.0 6 4 227 7 0.0 6 7 228 7 0.0 6 7 236 1 0.0 6 1 237 5 0.0 6 5 238 5 0.0 6 5 239 11 0.0 6 11 240 7 0.0 6 7 241 1 0.0 6 1 249 1 0.0 6 1 250 3 0.0 6 3 251 2 0.0 6 2 252 3 0.0 6 3 253 1 0.0 6 1 254 1 0.0 6 1 255 1 0.0 6 1 257 1 0.0 6 1 258 5 0.0 6 5 259 1 0.0 6 1 260 2 0.0 6 2 263 1 0.0 6 1 264 1 0.0 6 1 265 1 0.0 6 1 266 1 0.0 6 1 267 3 0.0 6 3 268 1 0.0 6 1 269 1 0.0 6 1 270 2 0.0 6 2 273 1 0.0 6 1 276 1 0.0 6 1 277 2 0.0 6 2 281 1 0.0 6 1 283 3 0.0 6 3 288 1 0.0 6 1 293 1 0.0 6 1 297 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 53492 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 53.9 1 1 6 1 13.5 1 0 1 9 1 0.2 2 1 10 3 0.1 3 2 1 11 1 0.0 3 0 1 12 2 0.0 3 2 13 5 0.0 3 5 14 9 0.0 4 8 0 0 0 0 0 1 15 1 0.0 4 1 16 9 0.0 4 7 2 17 12 0.0 5 12 18 7 0.0 5 5 2 19 11 0.0 5 9 2 20 39 0.0 6 38 1 21 32 0.0 6 31 1 22 23 0.0 6 22 1 23 24 0.0 6 23 1 24 17 0.0 6 16 1 25 17 0.0 6 15 1 1 26 14 0.0 6 14 27 16 0.0 6 13 3 28 12 0.0 6 11 1 29 14 0.0 6 12 0 1 0 0 1 30 10 0.0 6 8 1 1 31 15 0.0 6 14 1 32 7 0.0 6 7 33 17 0.0 6 16 1 34 13 0.0 6 13 35 17 0.0 6 14 2 1 36 11 0.0 6 11 37 7 0.0 6 6 0 1 38 11 0.0 6 8 3 39 10 0.0 6 10 40 13 0.0 6 12 1 41 10 0.0 6 10 42 11 0.0 6 10 1 43 11 0.0 6 9 2 44 16 0.0 6 16 45 14 0.0 6 14 46 7 0.0 6 7 47 18 0.0 6 16 1 1 48 18 0.0 6 17 1 49 17 0.0 6 17 50 20 0.0 6 19 1 51 25 0.0 6 23 2 52 22 0.0 6 20 2 53 28 0.0 6 27 1 54 16 0.0 6 15 1 55 16 0.0 6 13 3 56 22 0.0 6 21 0 1 57 24 0.0 6 21 2 0 0 0 1 58 32 0.0 6 32 59 16 0.0 6 16 60 19 0.0 6 18 1 61 19 0.0 6 15 1 2 1 62 27 0.0 6 25 1 1 63 29 0.0 6 28 1 64 28 0.0 6 27 1 65 20 0.0 6 18 2 66 43 0.0 6 41 2 67 120 0.0 6 116 2 1 1 68 50 0.0 6 48 2 69 60 0.0 6 58 2 70 31 0.0 6 29 2 71 46 0.0 6 45 1 72 42 0.0 6 41 1 73 33 0.0 6 32 1 74 42 0.0 6 41 1 75 35 0.0 6 34 1 76 40 0.0 6 38 2 77 37 0.0 6 36 1 78 30 0.0 6 28 1 1 79 35 0.0 6 33 2 80 54 0.0 6 52 2 81 54 0.0 6 54 82 54 0.0 6 51 3 83 66 0.0 6 61 4 0 0 0 0 1 84 47 0.0 6 47 85 65 0.0 6 63 2 86 40 0.0 6 38 1 0 1 87 64 0.0 6 63 1 88 51 0.0 6 47 4 89 69 0.0 6 68 0 0 0 0 1 90 58 0.0 6 52 5 1 91 50 0.0 6 48 1 1 92 61 0.0 6 59 2 93 61 0.0 6 60 1 94 61 0.0 6 59 2 95 80 0.0 6 79 1 96 84 0.0 6 82 2 97 69 0.0 6 64 5 98 63 0.0 6 60 3 99 62 0.0 6 59 3 100 75 0.0 6 74 1 101 82 0.0 6 78 4 102 82 0.0 6 77 4 1 103 88 0.0 6 85 3 104 146 0.0 6 141 2 2 1 105 109 0.0 6 105 2 0 1 1 106 114 0.0 6 110 1 1 0 0 0 2 107 77 0.0 6 75 1 1 108 114 0.0 6 113 1 109 117 0.0 6 115 2 110 126 0.0 6 121 5 111 121 0.0 6 115 6 112 150 0.0 6 147 3 113 126 0.0 6 118 6 1 0 0 0 1 114 141 0.0 6 140 1 115 138 0.0 6 137 1 116 152 0.0 6 145 4 0 1 1 1 117 164 0.0 6 159 4 0 0 0 0 1 118 160 0.0 6 154 6 119 197 0.0 6 192 3 0 1 1 120 170 0.0 6 166 4 121 174 0.0 6 168 5 0 0 0 1 122 168 0.0 6 164 4 123 207 0.0 6 200 5 1 0 1 124 218 0.0 6 214 3 0 1 125 291 0.0 6 287 3 0 1 126 269 0.0 6 264 3 1 0 0 0 1 127 280 0.0 6 270 7 1 0 1 0 1 128 303 0.0 6 299 4 129 320 0.0 6 316 3 0 1 130 369 0.0 6 364 4 0 1 131 402 0.0 6 391 10 0 0 0 1 132 375 0.0 6 369 3 0 0 0 1 2 133 402 0.0 6 396 6 134 474 0.0 6 466 5 1 0 1 1 135 442 0.0 6 439 3 136 397 0.0 6 388 8 0 0 0 0 1 137 469 0.0 6 460 7 1 0 1 138 429 0.0 6 424 5 139 464 0.0 6 459 4 0 0 0 0 1 140 551 0.0 6 541 8 0 0 0 1 1 141 693 0.0 6 681 8 2 0 2 142 927 0.0 6 919 8 143 837 0.0 6 830 5 1 0 0 0 1 144 937 0.0 6 930 7 145 1092 0.0 6 1086 6 146 1001 0.0 6 990 11 147 1302 0.0 6 1293 8 0 0 0 0 1 148 1221 0.0 6 1212 6 1 0 0 1 1 149 1071 0.0 6 1054 16 0 0 0 1 150 1655 0.0 6 1643 10 0 0 0 2 151 1397 0.0 6 1390 7 152 1612 0.0 6 1595 17 153 1458 0.0 6 1447 10 0 0 0 0 1 154 1020 0.0 6 1013 7 155 946 0.0 6 932 11 2 0 1 156 898 0.0 6 882 13 1 0 0 2 157 1153 0.0 6 1141 11 0 0 1 158 1173 0.0 6 1163 9 0 0 1 159 964 0.0 6 954 9 0 0 0 0 1 160 1179 0.0 6 1174 3 0 0 1 1 161 955 0.0 6 944 7 2 0 1 1 162 832 0.0 6 822 9 1 163 880 0.0 6 873 5 1 0 0 1 164 683 0.0 6 679 3 0 0 0 1 165 768 0.0 6 758 10 166 608 0.0 6 597 9 1 0 1 167 672 0.0 6 669 3 168 667 0.0 6 661 6 169 691 0.0 6 683 8 170 574 0.0 6 569 5 171 621 0.0 6 615 6 172 578 0.0 6 575 3 173 577 0.0 6 572 4 0 1 174 450 0.0 6 441 8 1 175 489 0.0 6 484 4 0 0 0 0 1 176 496 0.0 6 489 4 1 1 1 177 400 0.0 6 395 5 178 453 0.0 6 447 5 1 179 300 0.0 6 297 2 0 1 180 218 0.0 6 217 1 181 320 0.0 6 317 3 182 296 0.0 6 292 4 183 408 0.0 6 406 2 184 790 0.0 6 781 9 185 380 0.0 6 377 2 0 0 0 1 186 291 0.0 6 288 3 187 321 0.0 6 318 3 188 344 0.0 6 344 189 265 0.0 6 262 2 0 0 1 190 309 0.0 6 307 1 0 0 1 191 250 0.0 6 248 1 0 0 1 192 207 0.0 6 204 2 1 193 181 0.0 6 179 2 194 177 0.0 6 176 1 195 137 0.0 6 134 1 0 0 1 0 1 196 153 0.0 6 150 3 197 110 0.0 6 110 198 121 0.0 6 120 1 199 112 0.0 6 112 200 117 0.0 6 116 0 0 0 1 201 90 0.0 6 87 3 202 99 0.0 6 99 203 66 0.0 6 65 1 204 66 0.0 6 66 205 78 0.0 6 78 206 75 0.0 6 74 1 207 64 0.0 6 64 208 68 0.0 6 67 0 1 209 52 0.0 6 52 210 33 0.0 6 31 2 211 31 0.0 6 31 212 49 0.0 6 49 213 52 0.0 6 52 214 80 0.0 6 80 215 65 0.0 6 65 216 36 0.0 6 36 217 31 0.0 6 31 218 26 0.0 6 26 219 36 0.0 6 34 2 220 25 0.0 6 25 221 15 0.0 6 15 222 27 0.0 6 26 1 223 14 0.0 6 14 224 6 0.0 6 6 225 12 0.0 6 12 226 12 0.0 6 12 227 14 0.0 6 14 228 13 0.0 6 13 229 6 0.0 6 6 230 8 0.0 6 8 231 11 0.0 6 11 232 8 0.0 6 8 233 3 0.0 6 3 234 4 0.0 6 4 235 4 0.0 6 4 236 1 0.0 6 1 238 3 0.0 6 3 239 1 0.0 6 1 240 2 0.0 6 2 241 3 0.0 6 3 242 2 0.0 6 2 243 1 0.0 6 1 244 1 0.0 6 1 245 1 0.0 6 1 246 2 0.0 6 2 247 1 0.0 6 1 257 1 0.0 6 1 277 3 0.0 6 2 1 278 9 0.0 6 9 279 47 0.0 6 45 2 280 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1672 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 3 0.0 6 3 72 15 0.0 6 15 73 20 0.0 6 20 74 12 0.0 6 12 75 20 0.0 6 20 76 41 0.0 6 41 77 20 0.0 6 20 78 30 0.0 6 30 79 21 0.0 6 21 80 21 0.0 6 20 0 0 0 1 81 21 0.0 6 20 0 0 0 1 82 44 0.0 6 44 83 44 0.0 6 44 84 35 0.0 6 35 85 31 0.0 6 31 86 3 0.0 6 3 87 8 0.0 6 7 0 0 0 1 88 23 0.0 6 23 89 28 0.0 6 28 90 46 0.0 6 46 91 91 0.0 6 91 92 41 0.0 6 41 93 27 0.0 6 27 94 58 0.0 6 57 0 0 0 1 95 25 0.0 6 24 0 0 0 1 96 46 0.0 6 46 97 17 0.0 6 17 98 16 0.0 6 14 0 0 0 2 99 10 0.0 6 10 100 24 0.0 6 23 0 0 0 1 101 31 0.0 6 31 102 8 0.0 6 8 103 14 0.0 6 14 104 22 0.0 6 22 105 2 0.0 6 1 0 0 0 1 106 3 0.0 6 3 107 14 0.0 6 14 108 21 0.0 6 21 109 15 0.0 6 15 110 6 0.0 6 6 111 1 0.0 6 1 112 1 0.0 6 1 114 15 0.0 6 15 115 30 0.0 6 29 1 117 2 0.0 6 2 118 15 0.0 6 15 119 43 0.0 6 43 121 1 0.0 6 1 123 16 0.0 6 16 124 12 0.0 6 12 125 4 0.0 6 4 127 3 0.0 6 3 129 1 0.0 6 1 130 1 0.0 6 1 131 16 0.0 6 16 132 49 0.0 6 49 133 8 0.0 6 7 1 134 12 0.0 6 11 0 0 0 1 135 7 0.0 6 7 136 3 0.0 6 3 137 8 0.0 6 8 138 1 0.0 6 1 139 8 0.0 6 8 140 1 0.0 6 1 141 7 0.0 6 7 142 12 0.0 6 11 1 143 8 0.0 6 8 144 9 0.0 6 9 145 11 0.0 6 11 146 24 0.0 6 24 147 7 0.0 6 7 148 1 0.0 6 1 151 4 0.0 6 4 152 6 0.0 6 6 153 7 0.0 6 7 154 7 0.0 6 7 156 3 0.0 6 3 157 16 0.0 6 16 158 16 0.0 6 16 159 2 0.0 6 2 160 1 0.0 6 0 1 162 15 0.0 6 15 163 20 0.0 6 20 164 11 0.0 6 11 165 1 0.0 6 1 167 1 0.0 6 1 168 8 0.0 6 8 169 6 0.0 6 6 170 5 0.0 6 5 171 1 0.0 6 0 1 172 5 0.0 6 5 173 11 0.0 6 11 174 51 0.0 6 51 175 22 0.0 6 22 176 34 0.0 6 34 177 6 0.0 6 6 178 3 0.0 6 3 179 3 0.0 6 3 180 5 0.0 6 5 181 1 0.0 6 1 183 3 0.0 6 2 0 0 0 1 185 3 0.0 6 3 186 7 0.0 6 7 187 5 0.0 6 5 188 22 0.0 6 22 189 6 0.0 6 6 190 1 0.0 6 1 191 2 0.0 6 2 192 3 0.0 6 3 193 1 0.0 6 1 194 2 0.0 6 2 195 3 0.0 6 3 197 1 0.0 6 1 202 1 0.0 6 1 203 2 0.0 6 2 204 4 0.0 6 4 205 1 0.0 6 1 210 2 0.0 6 2 213 1 0.0 6 1 214 1 0.0 6 1 215 7 0.0 6 7 216 1 0.0 6 1 219 1 0.0 6 1 221 1 0.0 6 1 224 1 0.0 6 1 226 1 0.0 6 1 227 3 0.0 6 3 233 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_11_189_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_11_416_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_11_189_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_11_416_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.29 s (42 µs/read; 1.44 M reads/minute). === Summary === Total read pairs processed: 55,217 Read 1 with adapter: 55,217 (100.0%) Read 2 with adapter: 55,190 (100.0%) == Read fate breakdown == Pairs that were too short: 19 (0.0%) Pairs discarded as untrimmed: 27 (0.0%) Pairs written (passing filters): 55,171 (99.9%) Total basepairs processed: 24,078,992 bp Read 1: 13,444,277 bp Read 2: 10,634,715 bp Total written (filtered): 3,513,665 bp (14.6%) Read 1: 1,878,379 bp Read 2: 1,635,286 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 54031 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 53.9 1 1 6 5 13.5 1 2 0 3 7 2 3.4 2 0 0 2 8 2 0.8 2 2 9 1 0.2 2 1 10 1 0.1 3 1 14 2 0.0 4 2 15 1 0.0 4 1 16 1 0.0 4 0 1 19 2 0.0 5 1 1 20 45 0.0 6 44 1 21 26 0.0 6 25 1 22 21 0.0 6 19 1 1 23 19 0.0 6 19 24 11 0.0 6 11 25 14 0.0 6 12 2 26 7 0.0 6 7 27 5 0.0 6 4 0 0 0 0 1 28 6 0.0 6 6 29 8 0.0 6 8 30 6 0.0 6 6 31 3 0.0 6 3 32 4 0.0 6 4 33 2 0.0 6 2 34 6 0.0 6 6 35 5 0.0 6 5 36 2 0.0 6 1 1 37 2 0.0 6 2 38 6 0.0 6 6 39 2 0.0 6 2 40 2 0.0 6 2 41 10 0.0 6 8 2 42 6 0.0 6 5 1 43 2 0.0 6 2 44 5 0.0 6 5 45 4 0.0 6 4 46 4 0.0 6 4 47 3 0.0 6 2 1 48 5 0.0 6 5 49 7 0.0 6 5 1 0 0 0 0 1 50 3 0.0 6 3 51 4 0.0 6 4 52 7 0.0 6 7 53 2 0.0 6 1 1 54 3 0.0 6 3 55 5 0.0 6 5 56 3 0.0 6 3 57 4 0.0 6 3 1 58 4 0.0 6 3 1 59 1 0.0 6 0 0 1 60 3 0.0 6 3 61 2 0.0 6 2 62 11 0.0 6 11 63 4 0.0 6 4 64 4 0.0 6 4 65 2 0.0 6 2 66 3 0.0 6 3 67 1 0.0 6 1 68 3 0.0 6 3 69 11 0.0 6 10 0 0 1 70 8 0.0 6 8 71 4 0.0 6 3 1 72 4 0.0 6 3 0 0 0 0 1 73 6 0.0 6 6 74 7 0.0 6 6 1 75 4 0.0 6 4 76 3 0.0 6 3 77 8 0.0 6 7 1 78 4 0.0 6 4 79 3 0.0 6 3 80 11 0.0 6 10 1 81 6 0.0 6 6 82 7 0.0 6 7 83 9 0.0 6 9 84 5 0.0 6 5 85 12 0.0 6 12 86 10 0.0 6 10 87 6 0.0 6 6 88 5 0.0 6 5 89 6 0.0 6 6 90 5 0.0 6 5 91 2 0.0 6 2 92 5 0.0 6 5 93 6 0.0 6 6 94 9 0.0 6 9 95 7 0.0 6 6 1 96 5 0.0 6 5 97 3 0.0 6 3 98 7 0.0 6 7 99 10 0.0 6 10 100 11 0.0 6 11 101 12 0.0 6 12 102 14 0.0 6 13 1 103 20 0.0 6 19 1 104 14 0.0 6 13 1 105 11 0.0 6 11 106 15 0.0 6 15 107 18 0.0 6 18 108 17 0.0 6 17 109 15 0.0 6 14 1 110 11 0.0 6 11 111 17 0.0 6 17 112 19 0.0 6 19 113 12 0.0 6 11 1 114 10 0.0 6 10 115 15 0.0 6 14 0 0 1 116 21 0.0 6 21 117 17 0.0 6 17 118 20 0.0 6 20 119 13 0.0 6 13 120 12 0.0 6 11 1 121 14 0.0 6 14 122 25 0.0 6 24 1 123 19 0.0 6 19 124 24 0.0 6 24 125 16 0.0 6 15 1 126 23 0.0 6 23 127 21 0.0 6 21 128 22 0.0 6 20 2 129 26 0.0 6 26 130 29 0.0 6 29 131 18 0.0 6 18 132 26 0.0 6 26 133 28 0.0 6 27 1 134 36 0.0 6 36 135 23 0.0 6 23 136 35 0.0 6 35 137 62 0.0 6 62 138 53 0.0 6 53 139 72 0.0 6 72 140 400 0.0 6 398 2 141 349 0.0 6 348 1 142 363 0.0 6 362 1 143 403 0.0 6 402 1 144 406 0.0 6 406 145 404 0.0 6 403 1 146 333 0.0 6 331 2 147 397 0.0 6 397 148 549 0.0 6 547 1 0 0 1 149 445 0.0 6 444 1 150 549 0.0 6 549 151 342 0.0 6 342 152 202 0.0 6 200 2 153 304 0.0 6 304 154 241 0.0 6 240 1 155 326 0.0 6 324 2 156 227 0.0 6 226 1 157 277 0.0 6 276 1 158 311 0.0 6 310 1 159 389 0.0 6 386 2 0 0 0 1 160 272 0.0 6 271 1 161 258 0.0 6 257 1 162 259 0.0 6 258 1 163 290 0.0 6 289 1 164 206 0.0 6 206 165 222 0.0 6 222 166 263 0.0 6 263 167 174 0.0 6 173 1 168 261 0.0 6 259 2 169 219 0.0 6 215 3 0 0 1 170 333 0.0 6 333 171 286 0.0 6 286 172 227 0.0 6 227 173 256 0.0 6 255 0 0 0 0 0 1 174 307 0.0 6 307 175 256 0.0 6 256 176 245 0.0 6 245 177 265 0.0 6 265 178 587 0.0 6 587 179 255 0.0 6 254 0 0 0 0 1 180 220 0.0 6 218 2 181 290 0.0 6 290 182 262 0.0 6 261 1 183 195 0.0 6 194 1 184 234 0.0 6 234 185 273 0.0 6 272 1 186 239 0.0 6 237 2 187 282 0.0 6 279 3 188 266 0.0 6 265 1 189 305 0.0 6 305 190 333 0.0 6 333 191 344 0.0 6 342 1 0 1 192 400 0.0 6 398 2 193 448 0.0 6 443 4 0 1 194 412 0.0 6 411 1 195 415 0.0 6 414 1 196 646 0.0 6 646 197 550 0.0 6 542 8 198 416 0.0 6 412 3 1 199 443 0.0 6 441 1 1 200 397 0.0 6 393 3 0 0 0 1 201 339 0.0 6 337 2 202 598 0.0 6 598 203 380 0.0 6 380 204 418 0.0 6 415 3 205 576 0.0 6 568 7 1 206 413 0.0 6 410 3 207 506 0.0 6 506 208 389 0.0 6 388 1 209 489 0.0 6 486 3 210 509 0.0 6 505 4 211 584 0.0 6 581 3 212 537 0.0 6 530 7 213 576 0.0 6 570 6 214 716 0.0 6 712 4 215 518 0.0 6 514 3 0 0 0 0 1 216 542 0.0 6 538 4 217 572 0.0 6 567 4 0 1 218 457 0.0 6 452 3 1 0 0 1 219 419 0.0 6 416 2 0 0 0 1 220 424 0.0 6 418 6 221 370 0.0 6 369 1 222 461 0.0 6 456 5 223 392 0.0 6 390 2 224 542 0.0 6 541 1 225 487 0.0 6 485 2 226 517 0.0 6 513 3 1 227 422 0.0 6 417 5 228 383 0.0 6 382 1 229 486 0.0 6 484 2 230 402 0.0 6 400 2 231 408 0.0 6 407 1 232 376 0.0 6 375 1 233 338 0.0 6 336 1 0 0 0 0 1 234 302 0.0 6 300 1 0 0 0 1 235 339 0.0 6 338 1 236 286 0.0 6 285 1 237 308 0.0 6 306 2 238 324 0.0 6 321 3 239 317 0.0 6 315 2 240 373 0.0 6 370 3 241 371 0.0 6 368 3 242 305 0.0 6 301 4 243 295 0.0 6 294 0 0 0 1 244 253 0.0 6 250 3 245 235 0.0 6 235 246 203 0.0 6 201 2 247 240 0.0 6 238 2 248 165 0.0 6 163 2 249 190 0.0 6 189 1 250 250 0.0 6 248 2 251 197 0.0 6 192 5 252 340 0.0 6 336 4 253 166 0.0 6 162 4 254 208 0.0 6 207 1 255 186 0.0 6 185 1 256 227 0.0 6 222 5 257 248 0.0 6 242 6 258 258 0.0 6 253 5 259 274 0.0 6 268 5 1 260 173 0.0 6 173 261 199 0.0 6 197 1 0 0 0 1 262 149 0.0 6 147 2 263 224 0.0 6 217 6 1 264 137 0.0 6 136 1 265 142 0.0 6 140 2 266 138 0.0 6 138 267 218 0.0 6 215 3 268 184 0.0 6 180 2 1 1 269 173 0.0 6 171 2 270 118 0.0 6 115 3 271 175 0.0 6 174 1 272 212 0.0 6 208 3 0 0 1 273 210 0.0 6 205 5 274 250 0.0 6 246 3 1 275 257 0.0 6 250 7 276 677 0.0 6 663 13 0 1 277 1438 0.0 6 1426 11 0 1 278 5009 0.0 6 4898 107 1 1 0 0 2 279 89 0.0 6 88 1 280 4 0.0 6 3 0 1 281 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1186 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 6 215.7 1 6 63 1 0.0 6 1 64 113 0.0 6 108 5 70 1 0.0 6 1 71 2 0.0 6 2 72 7 0.0 6 7 73 14 0.0 6 14 74 8 0.0 6 8 75 8 0.0 6 8 76 10 0.0 6 10 77 13 0.0 6 13 78 9 0.0 6 9 79 7 0.0 6 7 80 10 0.0 6 10 81 12 0.0 6 12 82 13 0.0 6 13 83 11 0.0 6 11 84 9 0.0 6 9 85 5 0.0 6 5 86 14 0.0 6 14 87 7 0.0 6 7 88 10 0.0 6 10 89 6 0.0 6 6 90 11 0.0 6 11 91 11 0.0 6 11 92 12 0.0 6 12 93 8 0.0 6 8 94 7 0.0 6 7 95 9 0.0 6 9 96 6 0.0 6 6 97 5 0.0 6 5 98 5 0.0 6 5 99 3 0.0 6 3 100 2 0.0 6 2 101 6 0.0 6 6 102 6 0.0 6 6 103 4 0.0 6 4 104 5 0.0 6 5 105 3 0.0 6 3 106 13 0.0 6 13 107 7 0.0 6 7 108 5 0.0 6 5 109 3 0.0 6 3 110 12 0.0 6 12 111 10 0.0 6 10 112 5 0.0 6 5 113 9 0.0 6 9 114 2 0.0 6 2 115 3 0.0 6 3 116 1 0.0 6 1 117 6 0.0 6 6 118 6 0.0 6 6 119 7 0.0 6 7 120 3 0.0 6 3 121 5 0.0 6 5 122 4 0.0 6 4 123 1 0.0 6 1 124 10 0.0 6 10 125 13 0.0 6 13 126 2 0.0 6 2 127 2 0.0 6 2 128 8 0.0 6 8 129 11 0.0 6 11 130 5 0.0 6 5 131 4 0.0 6 4 132 3 0.0 6 3 133 8 0.0 6 8 134 8 0.0 6 8 135 14 0.0 6 14 136 6 0.0 6 6 137 3 0.0 6 3 138 9 0.0 6 9 139 2 0.0 6 2 140 6 0.0 6 6 141 5 0.0 6 5 142 5 0.0 6 5 143 4 0.0 6 4 144 9 0.0 6 9 145 11 0.0 6 11 146 4 0.0 6 4 147 10 0.0 6 10 148 7 0.0 6 7 149 6 0.0 6 6 150 4 0.0 6 4 151 8 0.0 6 8 152 7 0.0 6 7 153 1 0.0 6 1 154 5 0.0 6 5 155 3 0.0 6 3 156 6 0.0 6 6 157 9 0.0 6 9 158 4 0.0 6 4 159 12 0.0 6 12 160 6 0.0 6 6 161 6 0.0 6 6 162 7 0.0 6 7 163 9 0.0 6 9 164 8 0.0 6 8 165 12 0.0 6 12 166 6 0.0 6 6 167 6 0.0 6 6 168 9 0.0 6 9 169 4 0.0 6 4 170 12 0.0 6 12 171 11 0.0 6 11 172 5 0.0 6 5 173 6 0.0 6 6 174 5 0.0 6 5 175 6 0.0 6 6 176 8 0.0 6 8 177 6 0.0 6 6 178 5 0.0 6 5 179 3 0.0 6 3 180 3 0.0 6 3 181 4 0.0 6 4 182 6 0.0 6 6 183 6 0.0 6 6 184 1 0.0 6 1 185 3 0.0 6 3 186 2 0.0 6 2 187 7 0.0 6 7 188 6 0.0 6 6 189 6 0.0 6 6 190 4 0.0 6 4 191 3 0.0 6 3 192 8 0.0 6 8 193 1 0.0 6 1 194 3 0.0 6 3 195 6 0.0 6 6 196 6 0.0 6 6 197 4 0.0 6 4 198 4 0.0 6 4 199 4 0.0 6 4 200 1 0.0 6 1 201 5 0.0 6 5 202 4 0.0 6 4 203 5 0.0 6 5 204 12 0.0 6 12 205 5 0.0 6 5 206 3 0.0 6 3 207 5 0.0 6 5 208 1 0.0 6 1 209 6 0.0 6 6 210 5 0.0 6 5 211 6 0.0 6 6 212 5 0.0 6 5 213 7 0.0 6 7 214 3 0.0 6 3 215 6 0.0 6 6 216 4 0.0 6 4 217 4 0.0 6 4 218 6 0.0 6 6 219 3 0.0 6 3 220 3 0.0 6 3 221 6 0.0 6 6 222 3 0.0 6 3 223 4 0.0 6 4 224 3 0.0 6 3 227 4 0.0 6 4 228 6 0.0 6 6 229 3 0.0 6 3 230 5 0.0 6 5 231 1 0.0 6 1 232 3 0.0 6 3 233 4 0.0 6 4 234 3 0.0 6 3 235 5 0.0 6 5 236 1 0.0 6 1 237 1 0.0 6 1 238 2 0.0 6 2 239 1 0.0 6 1 240 1 0.0 6 1 241 7 0.0 6 7 242 5 0.0 6 5 243 8 0.0 6 8 244 1 0.0 6 1 245 2 0.0 6 2 246 1 0.0 6 1 247 2 0.0 6 2 248 4 0.0 6 4 249 4 0.0 6 4 250 2 0.0 6 2 251 2 0.0 6 2 253 2 0.0 6 2 254 2 0.0 6 2 255 1 0.0 6 1 256 1 0.0 6 1 258 1 0.0 6 1 259 2 0.0 6 2 260 3 0.0 6 3 264 1 0.0 6 1 265 1 0.0 6 1 266 3 0.0 6 3 267 1 0.0 6 1 268 1 0.0 6 1 270 2 0.0 6 2 272 3 0.0 6 3 274 1 0.0 6 1 276 1 0.0 6 1 278 1 0.0 6 1 282 1 0.0 6 1 288 1 0.0 6 1 292 1 0.0 6 1 299 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 54194 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 3.4 2 1 12 2 0.0 3 2 13 4 0.0 3 4 14 9 0.0 4 7 1 0 1 15 3 0.0 4 1 2 16 3 0.0 4 2 1 17 13 0.0 5 11 2 18 2 0.0 5 2 19 29 0.0 5 25 3 1 20 57 0.0 6 56 1 21 37 0.0 6 37 22 21 0.0 6 19 2 23 18 0.0 6 18 24 15 0.0 6 14 1 25 8 0.0 6 8 26 11 0.0 6 11 27 6 0.0 6 5 1 28 8 0.0 6 8 29 6 0.0 6 6 30 7 0.0 6 7 31 14 0.0 6 12 2 32 4 0.0 6 3 1 33 11 0.0 6 10 1 34 13 0.0 6 12 1 35 9 0.0 6 9 36 13 0.0 6 13 37 8 0.0 6 8 38 2 0.0 6 2 39 6 0.0 6 6 40 9 0.0 6 8 1 41 9 0.0 6 8 1 42 7 0.0 6 7 43 15 0.0 6 13 2 44 17 0.0 6 16 1 45 6 0.0 6 4 1 0 1 46 6 0.0 6 5 1 47 6 0.0 6 5 0 0 0 1 48 8 0.0 6 8 49 13 0.0 6 13 50 10 0.0 6 8 2 51 13 0.0 6 13 52 14 0.0 6 11 2 1 53 9 0.0 6 8 1 54 12 0.0 6 11 0 1 55 15 0.0 6 13 2 56 17 0.0 6 16 1 57 11 0.0 6 9 1 1 58 15 0.0 6 15 59 16 0.0 6 16 60 18 0.0 6 16 2 61 18 0.0 6 18 62 18 0.0 6 17 0 0 1 63 10 0.0 6 10 64 22 0.0 6 21 1 65 14 0.0 6 13 1 66 21 0.0 6 21 67 16 0.0 6 16 68 32 0.0 6 32 69 31 0.0 6 29 2 70 109 0.0 6 106 3 71 37 0.0 6 35 1 1 72 60 0.0 6 59 1 73 23 0.0 6 22 1 74 40 0.0 6 39 1 75 31 0.0 6 31 76 24 0.0 6 24 77 24 0.0 6 20 2 1 0 1 78 26 0.0 6 24 2 79 24 0.0 6 24 80 31 0.0 6 28 2 0 1 81 24 0.0 6 23 0 0 0 0 1 82 43 0.0 6 38 4 0 0 1 83 37 0.0 6 32 5 84 33 0.0 6 30 2 0 1 85 36 0.0 6 34 2 86 62 0.0 6 59 3 87 31 0.0 6 31 88 28 0.0 6 27 1 89 35 0.0 6 31 4 90 37 0.0 6 37 91 37 0.0 6 37 92 36 0.0 6 35 1 93 40 0.0 6 39 1 94 51 0.0 6 49 2 95 52 0.0 6 52 96 72 0.0 6 70 2 97 52 0.0 6 50 1 1 98 58 0.0 6 58 99 50 0.0 6 49 1 100 73 0.0 6 71 2 101 69 0.0 6 64 4 0 0 0 1 102 64 0.0 6 60 4 103 70 0.0 6 66 4 104 75 0.0 6 74 1 105 71 0.0 6 69 2 106 73 0.0 6 68 4 0 1 107 138 0.0 6 135 3 108 76 0.0 6 74 2 109 78 0.0 6 72 4 0 2 110 91 0.0 6 89 1 0 0 1 111 77 0.0 6 74 2 0 0 0 0 1 112 94 0.0 6 91 1 2 113 99 0.0 6 94 5 114 100 0.0 6 96 2 1 0 1 115 114 0.0 6 113 1 116 94 0.0 6 91 3 117 93 0.0 6 88 3 1 0 1 118 113 0.0 6 109 3 0 1 119 111 0.0 6 107 3 0 0 0 1 120 144 0.0 6 142 2 121 136 0.0 6 135 0 0 0 0 1 122 134 0.0 6 131 2 0 1 123 109 0.0 6 108 1 124 134 0.0 6 130 4 125 151 0.0 6 149 1 1 126 157 0.0 6 155 1 0 0 1 127 206 0.0 6 201 4 0 0 1 128 216 0.0 6 215 1 129 207 0.0 6 205 2 130 228 0.0 6 224 3 1 131 242 0.0 6 236 5 0 0 1 132 306 0.0 6 299 7 133 251 0.0 6 243 8 134 284 0.0 6 280 3 0 1 135 302 0.0 6 294 8 136 337 0.0 6 332 4 0 0 0 1 137 372 0.0 6 369 2 1 138 392 0.0 6 390 2 139 359 0.0 6 350 7 2 140 388 0.0 6 382 5 0 0 0 0 1 141 611 0.0 6 602 8 1 142 654 0.0 6 645 9 143 651 0.0 6 643 5 2 0 0 0 1 144 758 0.0 6 751 5 0 1 0 1 145 757 0.0 6 750 5 1 1 146 737 0.0 6 731 5 0 0 1 147 698 0.0 6 692 5 1 148 763 0.0 6 758 4 1 149 953 0.0 6 947 4 1 1 150 851 0.0 6 841 7 1 1 0 1 151 1074 0.0 6 1062 11 0 0 0 1 152 978 0.0 6 964 9 2 1 2 153 1107 0.0 6 1098 9 154 953 0.0 6 948 3 1 0 0 1 155 1033 0.0 6 1024 7 1 0 1 156 1008 0.0 6 994 13 0 0 1 157 824 0.0 6 813 9 0 0 1 0 1 158 932 0.0 6 922 8 0 1 0 1 159 822 0.0 6 816 5 1 160 933 0.0 6 918 12 2 0 0 0 1 161 821 0.0 6 815 4 0 0 0 1 1 162 763 0.0 6 759 4 163 774 0.0 6 766 8 164 657 0.0 6 648 6 0 1 1 0 1 165 615 0.0 6 610 4 0 0 1 166 725 0.0 6 709 14 1 0 1 167 585 0.0 6 581 3 0 1 168 587 0.0 6 582 5 169 770 0.0 6 757 13 170 545 0.0 6 540 4 0 0 0 1 171 732 0.0 6 728 3 0 0 1 172 609 0.0 6 608 0 0 0 1 173 605 0.0 6 603 2 174 676 0.0 6 670 5 1 175 743 0.0 6 732 9 2 176 685 0.0 6 676 9 177 626 0.0 6 617 8 0 0 0 0 1 178 584 0.0 6 576 7 0 0 1 179 652 0.0 6 648 3 0 0 1 180 528 0.0 6 513 13 0 1 0 0 1 181 538 0.0 6 533 3 0 0 2 182 420 0.0 6 418 2 183 416 0.0 6 410 6 184 347 0.0 6 341 5 0 1 185 316 0.0 6 316 186 550 0.0 6 549 1 187 642 0.0 6 631 9 1 1 188 674 0.0 6 665 9 189 469 0.0 6 465 4 190 531 0.0 6 525 6 191 496 0.0 6 490 5 0 1 192 399 0.0 6 390 5 0 1 0 1 2 193 394 0.0 6 392 2 194 390 0.0 6 384 6 195 334 0.0 6 328 6 196 575 0.0 6 565 9 0 0 0 0 1 197 250 0.0 6 246 3 0 0 0 0 1 198 374 0.0 6 369 5 199 383 0.0 6 373 9 0 0 1 200 296 0.0 6 292 4 201 374 0.0 6 367 7 202 254 0.0 6 247 7 203 239 0.0 6 233 5 1 204 236 0.0 6 232 4 205 229 0.0 6 222 7 206 189 0.0 6 186 3 207 175 0.0 6 171 3 1 208 288 0.0 6 283 5 209 117 0.0 6 117 210 182 0.0 6 177 4 0 0 1 211 239 0.0 6 232 7 212 175 0.0 6 172 3 213 75 0.0 6 74 1 214 148 0.0 6 146 2 215 196 0.0 6 190 5 0 1 216 187 0.0 6 184 2 0 0 1 217 147 0.0 6 147 218 104 0.0 6 102 2 219 90 0.0 6 88 2 220 47 0.0 6 46 1 221 106 0.0 6 104 2 222 76 0.0 6 75 1 223 53 0.0 6 50 3 224 64 0.0 6 63 1 225 76 0.0 6 76 226 110 0.0 6 104 6 227 185 0.0 6 180 5 228 129 0.0 6 126 3 229 145 0.0 6 139 6 230 227 0.0 6 222 5 231 71 0.0 6 67 4 232 253 0.0 6 244 9 233 42 0.0 6 39 3 234 120 0.0 6 118 2 235 79 0.0 6 77 2 236 51 0.0 6 51 237 64 0.0 6 64 238 33 0.0 6 33 239 6 0.0 6 6 240 15 0.0 6 13 1 0 0 0 1 241 51 0.0 6 49 0 1 0 1 242 27 0.0 6 25 2 243 14 0.0 6 14 244 28 0.0 6 27 0 0 0 1 245 64 0.0 6 62 2 246 20 0.0 6 20 247 27 0.0 6 27 248 22 0.0 6 21 1 249 35 0.0 6 34 1 250 16 0.0 6 16 251 56 0.0 6 55 1 252 38 0.0 6 37 1 253 7 0.0 6 7 254 65 0.0 6 64 1 255 6 0.0 6 6 256 6 0.0 6 6 257 1 0.0 6 1 258 1 0.0 6 1 259 1 0.0 6 1 263 2 0.0 6 2 266 1 0.0 6 1 273 1 0.0 6 1 280 1 0.0 6 1 281 14 0.0 6 13 0 1 282 82 0.0 6 80 1 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 996 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 2 0.0 6 2 67 1 0.0 6 1 68 3 0.0 6 3 69 9 0.0 6 9 70 15 0.0 6 15 71 12 0.0 6 12 72 11 0.0 6 11 73 13 0.0 6 13 74 10 0.0 6 9 0 0 0 1 75 9 0.0 6 9 76 16 0.0 6 16 77 17 0.0 6 16 1 78 20 0.0 6 20 79 19 0.0 6 19 80 11 0.0 6 11 81 4 0.0 6 4 82 13 0.0 6 13 83 8 0.0 6 8 84 18 0.0 6 18 85 10 0.0 6 10 86 5 0.0 6 5 87 13 0.0 6 13 88 12 0.0 6 12 89 4 0.0 6 4 90 6 0.0 6 6 91 4 0.0 6 4 92 6 0.0 6 6 93 7 0.0 6 7 94 5 0.0 6 5 95 7 0.0 6 7 96 5 0.0 6 5 97 4 0.0 6 4 98 5 0.0 6 4 1 99 9 0.0 6 9 100 6 0.0 6 6 101 8 0.0 6 8 102 8 0.0 6 8 103 6 0.0 6 6 104 9 0.0 6 9 105 16 0.0 6 16 106 9 0.0 6 9 107 17 0.0 6 17 108 3 0.0 6 3 109 2 0.0 6 2 110 8 0.0 6 8 111 3 0.0 6 3 112 4 0.0 6 4 113 6 0.0 6 6 114 3 0.0 6 3 115 2 0.0 6 2 116 7 0.0 6 7 117 6 0.0 6 6 118 1 0.0 6 1 119 5 0.0 6 5 120 33 0.0 6 33 121 4 0.0 6 4 122 10 0.0 6 10 123 5 0.0 6 5 124 7 0.0 6 7 125 9 0.0 6 9 126 5 0.0 6 5 127 3 0.0 6 3 128 9 0.0 6 9 129 8 0.0 6 8 130 7 0.0 6 7 131 15 0.0 6 15 132 4 0.0 6 4 133 6 0.0 6 6 134 13 0.0 6 11 2 135 6 0.0 6 6 136 6 0.0 6 6 137 7 0.0 6 7 138 9 0.0 6 9 139 8 0.0 6 8 140 11 0.0 6 11 141 11 0.0 6 10 0 0 0 1 142 6 0.0 6 6 143 8 0.0 6 8 144 9 0.0 6 9 145 6 0.0 6 6 146 4 0.0 6 4 147 20 0.0 6 20 148 10 0.0 6 10 149 12 0.0 6 12 150 6 0.0 6 6 151 9 0.0 6 9 152 5 0.0 6 5 153 2 0.0 6 2 154 6 0.0 6 6 155 8 0.0 6 8 156 8 0.0 6 8 157 6 0.0 6 6 158 6 0.0 6 6 159 10 0.0 6 10 160 6 0.0 6 6 161 10 0.0 6 10 162 6 0.0 6 5 1 163 8 0.0 6 8 164 7 0.0 6 7 165 7 0.0 6 7 166 4 0.0 6 4 167 8 0.0 6 7 1 168 9 0.0 6 9 169 6 0.0 6 6 170 5 0.0 6 5 171 4 0.0 6 4 172 12 0.0 6 12 173 5 0.0 6 5 174 5 0.0 6 5 175 2 0.0 6 2 176 1 0.0 6 1 177 7 0.0 6 6 1 178 13 0.0 6 13 179 3 0.0 6 3 180 3 0.0 6 3 181 3 0.0 6 3 182 6 0.0 6 6 183 3 0.0 6 3 184 5 0.0 6 5 185 4 0.0 6 4 186 2 0.0 6 2 187 2 0.0 6 2 188 7 0.0 6 7 189 2 0.0 6 2 190 2 0.0 6 2 191 2 0.0 6 2 192 6 0.0 6 6 193 4 0.0 6 4 194 1 0.0 6 1 197 2 0.0 6 2 198 3 0.0 6 3 199 1 0.0 6 1 200 3 0.0 6 3 201 2 0.0 6 2 202 4 0.0 6 4 203 3 0.0 6 3 204 1 0.0 6 1 206 2 0.0 6 2 207 1 0.0 6 1 208 2 0.0 6 2 209 1 0.0 6 1 210 1 0.0 6 1 211 1 0.0 6 1 212 2 0.0 6 2 213 1 0.0 6 1 214 2 0.0 6 2 216 1 0.0 6 1 218 1 0.0 6 1 228 1 0.0 6 1 230 1 0.0 6 1 248 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_12_190_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_12_417_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_12_190_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_12_417_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.21 s (43 µs/read; 1.38 M reads/minute). === Summary === Total read pairs processed: 51,033 Read 1 with adapter: 51,033 (100.0%) Read 2 with adapter: 50,987 (99.9%) == Read fate breakdown == Pairs that were too short: 33 (0.1%) Pairs discarded as untrimmed: 46 (0.1%) Pairs written (passing filters): 50,954 (99.8%) Total basepairs processed: 21,846,606 bp Read 1: 12,315,308 bp Read 2: 9,531,298 bp Total written (filtered): 3,347,119 bp (15.3%) Read 1: 1,734,875 bp Read 2: 1,612,244 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 49769 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 2 3.1 2 0 0 2 9 1 0.2 2 1 17 1 0.0 5 1 19 3 0.0 5 3 20 46 0.0 6 45 1 21 28 0.0 6 27 1 22 21 0.0 6 20 1 23 19 0.0 6 19 24 14 0.0 6 14 25 11 0.0 6 10 1 26 8 0.0 6 8 27 3 0.0 6 3 28 3 0.0 6 3 29 6 0.0 6 5 1 30 1 0.0 6 0 1 31 3 0.0 6 3 32 4 0.0 6 3 1 33 2 0.0 6 2 35 2 0.0 6 2 36 2 0.0 6 2 37 1 0.0 6 1 38 8 0.0 6 8 39 3 0.0 6 3 40 6 0.0 6 6 41 3 0.0 6 3 42 3 0.0 6 2 0 0 0 0 0 1 43 3 0.0 6 3 44 10 0.0 6 10 45 3 0.0 6 3 46 2 0.0 6 2 47 5 0.0 6 5 48 8 0.0 6 8 49 5 0.0 6 5 50 5 0.0 6 5 51 5 0.0 6 5 52 5 0.0 6 5 53 3 0.0 6 3 54 1 0.0 6 1 55 5 0.0 6 5 56 1 0.0 6 1 57 3 0.0 6 3 58 4 0.0 6 4 59 2 0.0 6 2 60 7 0.0 6 7 61 2 0.0 6 2 62 4 0.0 6 3 0 0 0 0 1 63 3 0.0 6 3 64 8 0.0 6 6 2 65 4 0.0 6 4 66 4 0.0 6 3 1 67 2 0.0 6 2 68 3 0.0 6 3 69 4 0.0 6 4 70 5 0.0 6 5 71 7 0.0 6 7 72 7 0.0 6 7 73 3 0.0 6 3 74 9 0.0 6 8 1 75 5 0.0 6 5 76 4 0.0 6 3 1 77 7 0.0 6 7 78 6 0.0 6 5 1 79 9 0.0 6 9 80 5 0.0 6 5 81 5 0.0 6 5 82 6 0.0 6 6 83 10 0.0 6 8 2 84 4 0.0 6 4 85 8 0.0 6 7 1 86 7 0.0 6 7 87 5 0.0 6 5 88 9 0.0 6 9 89 9 0.0 6 9 90 4 0.0 6 4 91 2 0.0 6 1 1 92 1 0.0 6 1 93 10 0.0 6 10 94 11 0.0 6 11 95 7 0.0 6 7 96 2 0.0 6 2 97 6 0.0 6 5 1 98 7 0.0 6 7 99 10 0.0 6 10 100 9 0.0 6 8 1 101 10 0.0 6 10 102 16 0.0 6 16 103 19 0.0 6 18 1 104 18 0.0 6 18 105 6 0.0 6 6 106 10 0.0 6 10 107 12 0.0 6 11 1 108 11 0.0 6 11 109 10 0.0 6 10 110 15 0.0 6 14 1 111 23 0.0 6 23 112 19 0.0 6 19 113 6 0.0 6 6 114 12 0.0 6 11 1 115 9 0.0 6 9 116 13 0.0 6 11 2 117 5 0.0 6 5 118 17 0.0 6 16 1 119 6 0.0 6 6 120 19 0.0 6 17 2 121 19 0.0 6 18 0 0 0 0 1 122 15 0.0 6 15 123 20 0.0 6 18 2 124 18 0.0 6 18 125 26 0.0 6 26 126 14 0.0 6 14 127 19 0.0 6 18 0 0 0 1 128 24 0.0 6 23 1 129 24 0.0 6 22 0 0 0 0 1 1 130 23 0.0 6 22 1 131 21 0.0 6 21 132 27 0.0 6 26 1 133 26 0.0 6 26 134 29 0.0 6 28 1 135 37 0.0 6 37 136 35 0.0 6 35 137 25 0.0 6 24 1 138 35 0.0 6 34 0 0 0 1 139 39 0.0 6 37 2 140 51 0.0 6 50 1 141 59 0.0 6 59 142 279 0.0 6 277 2 143 321 0.0 6 321 144 284 0.0 6 284 145 266 0.0 6 264 2 146 326 0.0 6 323 3 147 253 0.0 6 253 148 205 0.0 6 203 0 1 1 149 410 0.0 6 410 150 255 0.0 6 252 2 1 151 218 0.0 6 216 2 152 246 0.0 6 246 153 150 0.0 6 149 1 154 110 0.0 6 110 155 167 0.0 6 167 156 184 0.0 6 184 157 330 0.0 6 328 2 158 332 0.0 6 329 3 159 432 0.0 6 430 1 0 0 0 1 160 262 0.0 6 260 1 0 0 1 161 466 0.0 6 463 3 162 255 0.0 6 252 3 163 159 0.0 6 158 1 164 137 0.0 6 137 165 156 0.0 6 156 166 467 0.0 6 464 3 167 354 0.0 6 353 1 168 527 0.0 6 527 169 212 0.0 6 211 1 170 192 0.0 6 192 171 411 0.0 6 411 172 482 0.0 6 479 3 173 284 0.0 6 283 0 0 0 1 174 225 0.0 6 221 4 175 194 0.0 6 192 2 176 407 0.0 6 407 177 373 0.0 6 372 1 178 177 0.0 6 176 1 179 190 0.0 6 187 2 0 0 0 1 180 205 0.0 6 204 1 181 161 0.0 6 156 4 0 0 0 1 182 259 0.0 6 255 3 0 0 1 183 350 0.0 6 348 1 0 0 0 1 184 182 0.0 6 179 2 0 0 1 185 213 0.0 6 211 2 186 284 0.0 6 282 2 187 584 0.0 6 583 1 188 293 0.0 6 288 5 189 235 0.0 6 234 1 190 282 0.0 6 280 2 191 206 0.0 6 204 2 192 316 0.0 6 311 5 193 612 0.0 6 608 4 194 457 0.0 6 455 2 195 326 0.0 6 322 3 0 0 0 0 1 196 384 0.0 6 379 4 1 197 509 0.0 6 502 7 198 334 0.0 6 328 4 0 1 0 1 199 286 0.0 6 280 6 200 410 0.0 6 405 5 201 353 0.0 6 353 202 362 0.0 6 358 3 0 1 203 430 0.0 6 426 3 0 1 204 640 0.0 6 637 2 1 205 610 0.0 6 602 7 0 0 1 206 433 0.0 6 428 4 0 0 0 1 207 486 0.0 6 483 2 0 1 208 387 0.0 6 383 3 0 1 209 500 0.0 6 495 5 210 576 0.0 6 571 5 211 508 0.0 6 501 6 0 0 0 1 212 530 0.0 6 529 1 213 605 0.0 6 600 4 0 0 1 214 737 0.0 6 729 7 1 215 802 0.0 6 796 6 216 607 0.0 6 602 3 1 0 0 1 217 605 0.0 6 601 4 218 600 0.0 6 592 7 0 0 0 1 219 455 0.0 6 451 3 0 0 0 0 1 220 470 0.0 6 466 4 221 481 0.0 6 478 1 0 0 1 1 222 604 0.0 6 599 4 1 223 455 0.0 6 452 2 0 1 224 552 0.0 6 548 3 0 0 1 225 574 0.0 6 568 4 1 0 0 0 1 226 479 0.0 6 473 5 1 227 502 0.0 6 501 1 228 541 0.0 6 537 2 0 1 0 0 1 229 562 0.0 6 558 3 0 0 0 0 1 230 624 0.0 6 623 1 231 485 0.0 6 480 4 0 1 232 434 0.0 6 429 3 0 0 1 1 233 476 0.0 6 472 4 234 495 0.0 6 490 5 235 633 0.0 6 631 1 1 236 400 0.0 6 398 2 237 452 0.0 6 448 3 0 0 0 0 1 238 432 0.0 6 432 239 475 0.0 6 472 2 0 0 0 0 1 240 468 0.0 6 466 2 241 367 0.0 6 364 3 242 360 0.0 6 357 3 243 309 0.0 6 307 2 244 259 0.0 6 256 2 1 245 242 0.0 6 237 5 246 248 0.0 6 245 3 247 261 0.0 6 257 3 1 248 272 0.0 6 270 2 249 278 0.0 6 276 2 250 264 0.0 6 260 2 1 0 0 1 251 284 0.0 6 281 2 0 0 1 252 238 0.0 6 237 1 253 224 0.0 6 223 1 254 180 0.0 6 179 0 1 255 208 0.0 6 207 1 256 233 0.0 6 230 3 257 211 0.0 6 210 1 258 246 0.0 6 246 259 242 0.0 6 237 5 260 162 0.0 6 160 2 261 203 0.0 6 202 1 262 194 0.0 6 192 2 263 196 0.0 6 194 2 264 150 0.0 6 148 2 265 167 0.0 6 164 3 266 136 0.0 6 133 2 1 267 166 0.0 6 166 268 198 0.0 6 196 2 269 171 0.0 6 171 270 124 0.0 6 122 2 271 139 0.0 6 137 2 272 162 0.0 6 161 0 1 273 191 0.0 6 190 0 0 0 0 1 274 217 0.0 6 215 2 275 262 0.0 6 261 1 276 443 0.0 6 442 1 277 1061 0.0 6 1052 9 278 718 0.0 6 711 6 0 0 0 0 1 279 20 0.0 6 20 281 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1264 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 1 199.3 1 1 64 2 0.0 6 2 72 3 0.0 6 3 73 8 0.0 6 8 74 9 0.0 6 9 75 11 0.0 6 11 76 9 0.0 6 9 77 14 0.0 6 13 1 78 5 0.0 6 5 79 6 0.0 6 6 80 17 0.0 6 17 81 9 0.0 6 9 82 8 0.0 6 8 83 13 0.0 6 13 84 3 0.0 6 3 85 1 0.0 6 1 87 8 0.0 6 8 88 9 0.0 6 9 89 11 0.0 6 11 90 10 0.0 6 10 91 13 0.0 6 13 92 15 0.0 6 14 1 93 9 0.0 6 9 94 2 0.0 6 2 96 9 0.0 6 9 97 13 0.0 6 13 98 14 0.0 6 14 99 14 0.0 6 14 100 2 0.0 6 2 101 2 0.0 6 2 102 7 0.0 6 7 103 12 0.0 6 12 104 4 0.0 6 4 105 1 0.0 6 1 106 5 0.0 6 5 107 22 0.0 6 21 0 0 0 0 0 1 108 4 0.0 6 4 109 8 0.0 6 8 110 10 0.0 6 10 111 5 0.0 6 5 112 2 0.0 6 2 113 6 0.0 6 6 114 7 0.0 6 7 115 1 0.0 6 1 116 3 0.0 6 3 117 7 0.0 6 7 118 18 0.0 6 18 119 3 0.0 6 3 120 6 0.0 6 6 121 2 0.0 6 2 122 2 0.0 6 2 123 12 0.0 6 12 124 11 0.0 6 11 125 2 0.0 6 2 126 1 0.0 6 1 127 4 0.0 6 4 128 1 0.0 6 1 130 5 0.0 6 5 131 2 0.0 6 2 132 2 0.0 6 2 133 1 0.0 6 1 134 2 0.0 6 2 135 10 0.0 6 10 136 3 0.0 6 3 139 8 0.0 6 8 140 21 0.0 6 21 141 10 0.0 6 10 142 3 0.0 6 3 143 2 0.0 6 2 144 6 0.0 6 6 145 10 0.0 6 10 146 16 0.0 6 16 147 6 0.0 6 6 148 2 0.0 6 2 149 5 0.0 6 5 151 12 0.0 6 12 152 3 0.0 6 3 153 14 0.0 6 14 154 2 0.0 6 2 155 2 0.0 6 2 156 1 0.0 6 1 157 5 0.0 6 5 158 5 0.0 6 5 159 1 0.0 6 1 160 4 0.0 6 4 161 3 0.0 6 3 162 5 0.0 6 5 163 4 0.0 6 4 164 10 0.0 6 10 166 2 0.0 6 2 167 11 0.0 6 11 168 20 0.0 6 20 169 12 0.0 6 12 170 14 0.0 6 14 171 7 0.0 6 7 172 9 0.0 6 9 173 3 0.0 6 3 174 3 0.0 6 3 175 4 0.0 6 4 176 5 0.0 6 5 177 2 0.0 6 2 178 8 0.0 6 8 179 5 0.0 6 5 180 12 0.0 6 12 181 5 0.0 6 5 182 6 0.0 6 6 183 7 0.0 6 7 184 10 0.0 6 10 185 7 0.0 6 7 186 9 0.0 6 9 187 11 0.0 6 11 188 13 0.0 6 13 189 7 0.0 6 7 190 3 0.0 6 3 191 11 0.0 6 11 192 14 0.0 6 14 193 16 0.0 6 16 194 25 0.0 6 25 195 15 0.0 6 15 196 3 0.0 6 3 197 6 0.0 6 6 198 10 0.0 6 10 199 37 0.0 6 37 200 5 0.0 6 5 201 1 0.0 6 1 202 8 0.0 6 8 203 9 0.0 6 9 204 20 0.0 6 20 205 10 0.0 6 10 206 7 0.0 6 7 207 2 0.0 6 2 208 3 0.0 6 3 210 2 0.0 6 2 211 1 0.0 6 1 212 1 0.0 6 1 213 5 0.0 6 5 214 2 0.0 6 2 215 1 0.0 6 1 216 2 0.0 6 2 217 8 0.0 6 8 218 3 0.0 6 3 219 5 0.0 6 5 220 4 0.0 6 4 221 11 0.0 6 11 222 1 0.0 6 1 223 2 0.0 6 2 224 3 0.0 6 3 225 2 0.0 6 2 226 3 0.0 6 3 227 7 0.0 6 7 228 1 0.0 6 1 230 5 0.0 6 5 231 6 0.0 6 6 232 7 0.0 6 7 233 5 0.0 6 5 234 3 0.0 6 3 235 2 0.0 6 2 236 2 0.0 6 2 237 3 0.0 6 3 238 5 0.0 6 5 239 3 0.0 6 3 243 1 0.0 6 1 244 3 0.0 6 3 245 7 0.0 6 7 246 9 0.0 6 9 248 7 0.0 6 7 249 6 0.0 6 6 250 4 0.0 6 4 251 10 0.0 6 10 252 23 0.0 6 23 253 4 0.0 6 4 255 1 0.0 6 1 256 1 0.0 6 1 257 2 0.0 6 2 258 3 0.0 6 3 259 5 0.0 6 5 260 3 0.0 6 3 261 2 0.0 6 2 262 1 0.0 6 1 263 1 0.0 6 1 264 1 0.0 6 1 265 4 0.0 6 4 266 4 0.0 6 4 267 1 0.0 6 1 268 11 0.0 6 11 269 2 0.0 6 2 271 1 0.0 6 1 274 1 0.0 6 1 276 2 0.0 6 2 277 10 0.0 6 10 278 1 0.0 6 1 280 8 0.0 6 8 281 2 0.0 6 2 282 1 0.0 6 1 284 1 0.0 6 1 285 2 0.0 6 2 286 2 0.0 6 2 289 2 0.0 6 2 290 2 0.0 6 2 296 1 0.0 6 1 297 2 0.0 6 2 298 1 0.0 6 1 299 1 0.0 6 1 300 3 0.0 6 2 1 306 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 49844 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 797.4 0 1 7 2 3.1 2 1 1 8 1 0.8 2 1 10 2 0.0 3 2 11 4 0.0 3 3 1 12 5 0.0 3 5 13 9 0.0 3 8 1 14 11 0.0 4 9 1 1 15 1 0.0 4 1 16 2 0.0 4 1 1 17 36 0.0 5 32 4 18 17 0.0 5 15 2 19 15 0.0 5 13 2 20 51 0.0 6 47 4 21 35 0.0 6 34 1 22 28 0.0 6 25 2 0 1 23 12 0.0 6 11 1 24 8 0.0 6 8 25 11 0.0 6 11 26 18 0.0 6 16 2 27 4 0.0 6 3 1 28 9 0.0 6 8 1 29 19 0.0 6 17 2 30 10 0.0 6 9 0 0 0 1 31 12 0.0 6 10 1 0 1 32 9 0.0 6 8 1 33 3 0.0 6 3 34 12 0.0 6 10 1 0 0 0 1 35 4 0.0 6 4 36 8 0.0 6 6 2 37 5 0.0 6 4 0 1 38 16 0.0 6 15 1 39 11 0.0 6 10 1 40 15 0.0 6 15 41 10 0.0 6 9 1 42 12 0.0 6 11 0 1 43 9 0.0 6 8 0 0 1 44 14 0.0 6 12 1 1 45 15 0.0 6 13 2 46 10 0.0 6 10 47 12 0.0 6 12 48 14 0.0 6 13 1 49 17 0.0 6 16 0 0 0 1 50 23 0.0 6 18 5 51 11 0.0 6 10 1 52 9 0.0 6 8 1 53 20 0.0 6 20 54 19 0.0 6 17 1 0 0 0 1 55 22 0.0 6 21 0 0 0 1 56 13 0.0 6 12 1 57 17 0.0 6 15 2 58 20 0.0 6 17 3 59 22 0.0 6 21 1 60 19 0.0 6 14 4 1 61 19 0.0 6 18 1 62 24 0.0 6 23 1 63 11 0.0 6 10 1 64 21 0.0 6 20 1 65 22 0.0 6 21 1 66 27 0.0 6 26 0 1 67 38 0.0 6 36 1 0 1 68 104 0.0 6 102 1 0 0 0 1 69 54 0.0 6 48 4 0 0 2 70 60 0.0 6 58 2 71 32 0.0 6 31 0 0 0 0 1 72 37 0.0 6 35 1 1 73 32 0.0 6 30 2 74 45 0.0 6 43 2 75 37 0.0 6 36 0 0 0 1 76 29 0.0 6 27 1 0 0 1 77 34 0.0 6 34 78 26 0.0 6 23 2 1 79 65 0.0 6 59 6 80 31 0.0 6 30 1 81 51 0.0 6 51 82 45 0.0 6 42 3 83 50 0.0 6 49 0 0 0 0 0 1 84 38 0.0 6 33 3 0 0 0 2 85 42 0.0 6 39 3 86 35 0.0 6 33 2 87 40 0.0 6 37 3 88 47 0.0 6 44 1 0 1 0 0 1 89 45 0.0 6 43 2 90 37 0.0 6 34 2 0 0 0 1 91 67 0.0 6 67 92 58 0.0 6 56 2 93 60 0.0 6 54 6 94 57 0.0 6 53 3 0 0 1 95 50 0.0 6 49 1 96 63 0.0 6 60 3 97 73 0.0 6 71 2 98 57 0.0 6 53 3 0 0 0 1 99 51 0.0 6 47 2 0 1 1 100 60 0.0 6 60 101 78 0.0 6 76 1 1 102 73 0.0 6 72 1 103 63 0.0 6 62 1 104 78 0.0 6 76 2 105 141 0.0 6 137 3 1 106 89 0.0 6 85 2 0 0 0 2 107 96 0.0 6 93 3 108 88 0.0 6 85 2 1 109 96 0.0 6 94 1 0 0 0 0 1 110 95 0.0 6 91 3 0 0 0 0 1 111 86 0.0 6 83 3 112 117 0.0 6 115 2 113 148 0.0 6 146 1 0 0 0 0 1 114 116 0.0 6 113 3 115 121 0.0 6 120 1 116 137 0.0 6 132 3 0 0 0 1 1 117 105 0.0 6 104 0 0 0 1 118 146 0.0 6 140 4 0 1 1 119 130 0.0 6 126 4 120 147 0.0 6 145 2 121 142 0.0 6 137 4 1 122 133 0.0 6 130 3 123 146 0.0 6 145 1 124 172 0.0 6 164 7 0 0 0 1 125 206 0.0 6 202 4 126 216 0.0 6 215 1 127 235 0.0 6 229 4 1 1 128 225 0.0 6 219 4 0 0 1 1 129 241 0.0 6 237 3 1 130 267 0.0 6 261 5 0 0 0 1 131 305 0.0 6 295 8 0 1 0 0 1 132 337 0.0 6 328 9 133 329 0.0 6 323 4 0 1 1 134 393 0.0 6 383 3 1 2 0 3 1 135 403 0.0 6 398 4 1 136 349 0.0 6 345 3 1 137 348 0.0 6 338 8 1 0 1 138 338 0.0 6 327 9 1 0 1 139 332 0.0 6 326 5 0 0 1 140 543 0.0 6 531 11 0 0 0 1 141 623 0.0 6 611 9 0 0 0 2 1 142 754 0.0 6 741 10 2 1 143 734 0.0 6 721 13 144 640 0.0 6 630 7 0 1 0 1 1 145 797 0.0 6 782 13 0 0 0 1 1 146 689 0.0 6 676 9 2 2 147 709 0.0 6 698 5 1 0 0 4 1 148 933 0.0 6 912 17 2 0 0 1 1 149 964 0.0 6 943 16 1 2 2 150 923 0.0 6 903 14 0 2 1 2 1 151 1274 0.0 6 1248 20 0 1 1 1 3 152 939 0.0 6 921 17 1 153 1022 0.0 6 998 19 2 1 1 0 1 154 1047 0.0 6 1027 15 0 2 1 1 1 155 886 0.0 6 873 12 0 0 0 0 1 156 1004 0.0 6 987 15 1 0 0 1 157 924 0.0 6 909 14 0 0 0 1 158 1010 0.0 6 989 19 0 0 1 1 159 954 0.0 6 942 7 2 1 1 0 1 160 970 0.0 6 951 18 0 0 1 161 841 0.0 6 831 8 0 0 0 2 162 714 0.0 6 700 11 1 1 0 0 1 163 623 0.0 6 606 13 1 0 1 0 2 164 693 0.0 6 683 8 1 0 0 1 165 652 0.0 6 644 5 0 0 2 1 166 748 0.0 6 731 15 0 1 1 167 831 0.0 6 823 5 0 0 1 0 2 168 626 0.0 6 611 12 0 1 0 2 169 823 0.0 6 807 13 0 2 0 1 170 675 0.0 6 665 9 0 0 0 0 1 171 712 0.0 6 701 10 0 0 1 172 630 0.0 6 619 9 0 0 1 1 173 625 0.0 6 618 4 2 0 0 0 1 174 598 0.0 6 593 5 175 622 0.0 6 616 5 0 1 176 566 0.0 6 558 6 1 0 0 1 177 531 0.0 6 528 2 0 0 1 178 481 0.0 6 475 3 1 0 0 1 1 179 517 0.0 6 509 8 180 370 0.0 6 365 5 181 396 0.0 6 389 2 1 0 2 2 182 353 0.0 6 349 3 0 0 0 1 183 371 0.0 6 368 3 184 426 0.0 6 419 7 185 653 0.0 6 642 10 0 0 1 186 610 0.0 6 603 6 1 187 383 0.0 6 380 1 1 0 0 0 1 188 401 0.0 6 400 1 189 316 0.0 6 309 5 0 1 0 1 190 366 0.0 6 358 7 1 191 383 0.0 6 380 3 192 311 0.0 6 307 4 193 258 0.0 6 257 1 194 223 0.0 6 221 2 195 170 0.0 6 170 196 175 0.0 6 173 2 197 247 0.0 6 245 1 0 0 1 198 162 0.0 6 157 2 0 1 1 0 1 199 162 0.0 6 160 1 0 1 200 178 0.0 6 176 2 201 151 0.0 6 149 2 202 132 0.0 6 129 3 203 170 0.0 6 168 2 204 125 0.0 6 123 2 205 128 0.0 6 126 2 206 124 0.0 6 122 2 207 128 0.0 6 125 3 208 193 0.0 6 189 4 209 69 0.0 6 67 2 210 91 0.0 6 87 4 211 55 0.0 6 55 212 88 0.0 6 87 1 213 109 0.0 6 107 1 0 0 1 214 145 0.0 6 138 6 0 1 215 96 0.0 6 95 1 216 42 0.0 6 42 217 53 0.0 6 52 1 218 38 0.0 6 38 219 58 0.0 6 58 220 27 0.0 6 26 1 221 25 0.0 6 25 222 42 0.0 6 42 223 19 0.0 6 19 224 15 0.0 6 15 225 19 0.0 6 19 226 18 0.0 6 17 1 227 25 0.0 6 25 228 12 0.0 6 12 229 9 0.0 6 9 230 6 0.0 6 5 1 231 8 0.0 6 8 232 8 0.0 6 8 233 5 0.0 6 5 234 8 0.0 6 8 235 2 0.0 6 2 236 5 0.0 6 5 237 2 0.0 6 2 238 5 0.0 6 5 239 1 0.0 6 1 240 3 0.0 6 3 241 5 0.0 6 5 242 6 0.0 6 6 243 1 0.0 6 1 244 4 0.0 6 4 245 2 0.0 6 2 247 2 0.0 6 2 248 1 0.0 6 1 249 1 0.0 6 1 250 1 0.0 6 1 279 9 0.0 6 8 1 280 36 0.0 6 36 281 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1143 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 3 0.0 6 3 71 13 0.0 6 13 72 12 0.0 6 12 73 15 0.0 6 15 74 9 0.0 6 9 75 16 0.0 6 15 1 76 4 0.0 6 4 77 4 0.0 6 4 78 31 0.0 6 31 79 10 0.0 6 10 80 15 0.0 6 14 0 0 0 1 81 6 0.0 6 6 82 3 0.0 6 3 83 2 0.0 6 2 84 10 0.0 6 10 85 9 0.0 6 9 86 16 0.0 6 16 87 13 0.0 6 13 88 16 0.0 6 16 89 11 0.0 6 11 90 19 0.0 6 19 91 7 0.0 6 7 92 7 0.0 6 7 93 2 0.0 6 2 94 6 0.0 6 6 95 17 0.0 6 17 96 14 0.0 6 14 97 17 0.0 6 17 98 8 0.0 6 8 99 7 0.0 6 7 100 18 0.0 6 18 101 13 0.0 6 13 102 13 0.0 6 12 0 0 0 1 103 7 0.0 6 7 104 4 0.0 6 4 105 22 0.0 6 22 106 11 0.0 6 11 107 4 0.0 6 4 108 9 0.0 6 8 0 0 1 109 8 0.0 6 8 110 3 0.0 6 3 111 6 0.0 6 6 112 10 0.0 6 10 113 1 0.0 6 1 114 7 0.0 6 7 115 12 0.0 6 12 116 32 0.0 6 32 117 6 0.0 6 6 118 8 0.0 6 8 119 4 0.0 6 3 0 0 0 1 121 6 0.0 6 6 122 31 0.0 6 31 123 5 0.0 6 5 124 1 0.0 6 1 125 1 0.0 6 1 126 4 0.0 6 4 128 1 0.0 6 1 129 2 0.0 6 2 130 5 0.0 6 5 131 4 0.0 6 4 132 7 0.0 6 7 133 9 0.0 6 9 134 1 0.0 6 1 135 9 0.0 6 9 136 1 0.0 6 1 137 8 0.0 6 8 138 19 0.0 6 19 139 8 0.0 6 8 140 8 0.0 6 8 141 5 0.0 6 5 142 9 0.0 6 8 1 143 11 0.0 6 11 144 36 0.0 6 36 145 4 0.0 6 4 146 5 0.0 6 5 147 11 0.0 6 11 148 1 0.0 6 1 149 10 0.0 6 10 150 7 0.0 6 7 151 14 0.0 6 14 153 4 0.0 6 4 154 3 0.0 6 3 155 5 0.0 6 5 156 3 0.0 6 3 157 4 0.0 6 4 158 1 0.0 6 1 159 2 0.0 6 2 160 23 0.0 6 22 1 161 1 0.0 6 1 162 15 0.0 6 15 163 10 0.0 6 10 164 3 0.0 6 3 165 5 0.0 6 5 166 19 0.0 6 19 167 13 0.0 6 13 168 15 0.0 6 14 1 169 10 0.0 6 10 170 8 0.0 6 8 172 1 0.0 6 1 173 1 0.0 6 1 174 5 0.0 6 5 175 3 0.0 6 3 176 4 0.0 6 4 177 12 0.0 6 12 178 9 0.0 6 9 179 9 0.0 6 9 180 5 0.0 6 5 181 5 0.0 6 5 182 9 0.0 6 9 183 2 0.0 6 2 184 3 0.0 6 3 185 9 0.0 6 8 1 186 5 0.0 6 5 187 12 0.0 6 12 188 6 0.0 6 6 189 5 0.0 6 5 190 5 0.0 6 5 191 6 0.0 6 6 192 22 0.0 6 22 193 5 0.0 6 5 194 2 0.0 6 2 195 1 0.0 6 0 1 196 9 0.0 6 9 197 26 0.0 6 26 198 4 0.0 6 4 199 4 0.0 6 4 200 5 0.0 6 5 201 6 0.0 6 6 202 3 0.0 6 3 203 2 0.0 6 2 204 2 0.0 6 2 205 4 0.0 6 4 206 6 0.0 6 6 207 1 0.0 6 1 208 1 0.0 6 1 210 1 0.0 6 1 212 2 0.0 6 2 215 2 0.0 6 2 219 2 0.0 6 2 221 1 0.0 6 1 222 1 0.0 6 1 223 1 0.0 6 1 225 1 0.0 6 1 229 1 0.0 6 1 249 1 0.0 6 1 250 4 0.0 6 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_13_191_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_13_418_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_13_191_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_13_418_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.50 s (51 µs/read; 1.18 M reads/minute). === Summary === Total read pairs processed: 49,122 Read 1 with adapter: 49,122 (100.0%) Read 2 with adapter: 49,073 (99.9%) == Read fate breakdown == Pairs that were too short: 30 (0.1%) Pairs discarded as untrimmed: 49 (0.1%) Pairs written (passing filters): 49,043 (99.8%) Total basepairs processed: 20,736,533 bp Read 1: 11,468,468 bp Read 2: 9,268,065 bp Total written (filtered): 3,233,873 bp (15.6%) Read 1: 1,694,903 bp Read 2: 1,538,970 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 47562 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 191.9 1 1 6 2 12.0 1 1 0 1 7 4 3.0 2 2 1 1 8 2 0.7 2 2 15 1 0.0 4 1 16 2 0.0 4 2 17 5 0.0 5 5 18 1 0.0 5 0 1 20 41 0.0 6 41 21 16 0.0 6 15 1 22 12 0.0 6 12 23 22 0.0 6 21 1 24 12 0.0 6 10 1 1 25 14 0.0 6 13 1 26 6 0.0 6 4 1 1 27 7 0.0 6 6 1 28 7 0.0 6 7 29 6 0.0 6 6 30 6 0.0 6 5 1 31 9 0.0 6 9 32 4 0.0 6 4 33 1 0.0 6 1 34 6 0.0 6 6 35 3 0.0 6 2 1 36 2 0.0 6 2 37 5 0.0 6 5 38 1 0.0 6 1 39 4 0.0 6 3 0 0 1 40 2 0.0 6 2 41 5 0.0 6 4 1 43 2 0.0 6 2 44 5 0.0 6 5 45 3 0.0 6 3 46 4 0.0 6 4 47 4 0.0 6 3 1 48 4 0.0 6 3 1 49 6 0.0 6 5 1 50 5 0.0 6 4 0 0 1 51 4 0.0 6 4 52 4 0.0 6 4 53 3 0.0 6 3 54 6 0.0 6 4 2 55 3 0.0 6 3 56 3 0.0 6 3 57 6 0.0 6 6 58 3 0.0 6 3 59 1 0.0 6 1 61 4 0.0 6 4 62 5 0.0 6 5 63 3 0.0 6 2 1 64 4 0.0 6 3 1 65 10 0.0 6 10 66 5 0.0 6 5 67 6 0.0 6 5 0 1 68 2 0.0 6 1 1 69 17 0.0 6 17 70 8 0.0 6 8 71 8 0.0 6 8 72 6 0.0 6 6 73 7 0.0 6 7 74 3 0.0 6 3 75 8 0.0 6 8 76 5 0.0 6 4 1 77 6 0.0 6 6 78 6 0.0 6 5 1 79 7 0.0 6 7 80 5 0.0 6 5 81 7 0.0 6 7 82 8 0.0 6 8 83 7 0.0 6 7 85 4 0.0 6 4 86 8 0.0 6 8 87 7 0.0 6 7 88 6 0.0 6 6 89 13 0.0 6 13 90 10 0.0 6 10 91 11 0.0 6 11 92 7 0.0 6 6 1 93 4 0.0 6 4 94 7 0.0 6 7 95 5 0.0 6 5 96 3 0.0 6 2 1 97 6 0.0 6 6 98 11 0.0 6 10 1 99 7 0.0 6 7 100 16 0.0 6 16 101 6 0.0 6 6 102 15 0.0 6 15 103 17 0.0 6 15 2 104 22 0.0 6 21 1 105 11 0.0 6 10 1 106 8 0.0 6 8 107 13 0.0 6 13 108 15 0.0 6 15 109 10 0.0 6 10 110 20 0.0 6 19 1 111 15 0.0 6 15 112 11 0.0 6 10 0 1 113 8 0.0 6 8 114 17 0.0 6 16 0 0 0 0 1 115 18 0.0 6 18 116 16 0.0 6 15 1 117 14 0.0 6 13 1 118 19 0.0 6 19 119 12 0.0 6 11 1 120 14 0.0 6 14 121 15 0.0 6 15 122 15 0.0 6 15 123 11 0.0 6 10 1 124 23 0.0 6 23 125 15 0.0 6 15 126 17 0.0 6 17 127 20 0.0 6 19 1 128 24 0.0 6 23 1 129 23 0.0 6 22 1 130 25 0.0 6 25 131 26 0.0 6 26 132 32 0.0 6 30 2 133 31 0.0 6 31 134 39 0.0 6 39 135 36 0.0 6 36 136 40 0.0 6 40 137 41 0.0 6 41 138 58 0.0 6 58 139 71 0.0 6 71 140 115 0.0 6 114 1 141 607 0.0 6 600 7 142 219 0.0 6 219 143 761 0.0 6 758 3 144 511 0.0 6 510 1 145 240 0.0 6 239 1 146 903 0.0 6 901 2 147 986 0.0 6 986 148 311 0.0 6 310 0 0 0 1 149 223 0.0 6 221 2 150 176 0.0 6 173 2 0 0 0 0 1 151 1132 0.0 6 1130 1 0 0 0 1 152 210 0.0 6 209 1 153 87 0.0 6 85 2 154 120 0.0 6 119 1 155 514 0.0 6 511 3 156 134 0.0 6 132 2 157 104 0.0 6 104 158 115 0.0 6 115 159 125 0.0 6 125 160 66 0.0 6 66 161 95 0.0 6 92 2 0 0 0 0 1 162 93 0.0 6 92 1 163 104 0.0 6 103 1 164 110 0.0 6 109 1 165 172 0.0 6 171 1 166 80 0.0 6 80 167 88 0.0 6 85 3 168 191 0.0 6 191 169 183 0.0 6 183 170 888 0.0 6 886 2 171 1207 0.0 6 1204 2 0 0 0 0 1 172 194 0.0 6 194 173 323 0.0 6 321 2 174 108 0.0 6 108 175 129 0.0 6 128 0 1 176 143 0.0 6 141 2 177 84 0.0 6 82 2 178 156 0.0 6 156 179 205 0.0 6 205 180 102 0.0 6 102 181 118 0.0 6 118 182 154 0.0 6 148 5 1 183 144 0.0 6 142 2 184 150 0.0 6 148 2 185 223 0.0 6 222 1 186 199 0.0 6 197 2 187 435 0.0 6 432 3 188 250 0.0 6 248 2 189 441 0.0 6 436 5 190 2157 0.0 6 2150 7 191 1935 0.0 6 1927 8 192 466 0.0 6 463 3 193 317 0.0 6 316 1 194 340 0.0 6 337 3 195 371 0.0 6 366 4 0 1 196 361 0.0 6 354 5 1 0 0 0 1 197 495 0.0 6 491 3 1 198 690 0.0 6 688 2 199 256 0.0 6 253 3 200 256 0.0 6 254 1 1 201 344 0.0 6 341 3 202 497 0.0 6 494 3 203 636 0.0 6 631 5 204 1184 0.0 6 1180 4 205 668 0.0 6 663 4 0 0 0 0 1 206 319 0.0 6 317 1 1 207 454 0.0 6 449 4 0 0 1 208 382 0.0 6 379 2 0 0 0 0 1 209 232 0.0 6 224 8 210 284 0.0 6 279 5 211 302 0.0 6 297 5 212 285 0.0 6 282 3 213 424 0.0 6 418 6 214 541 0.0 6 530 10 1 215 414 0.0 6 408 5 0 1 216 442 0.0 6 437 3 0 1 0 1 217 404 0.0 6 398 6 218 459 0.0 6 457 2 219 430 0.0 6 427 3 220 343 0.0 6 339 4 221 550 0.0 6 542 8 222 745 0.0 6 743 2 223 333 0.0 6 332 1 224 524 0.0 6 521 3 225 335 0.0 6 332 2 0 0 0 0 1 226 307 0.0 6 302 5 227 240 0.0 6 237 3 228 202 0.0 6 197 5 229 235 0.0 6 232 2 0 0 0 0 1 230 237 0.0 6 233 4 231 324 0.0 6 322 2 232 320 0.0 6 318 2 233 329 0.0 6 327 2 234 348 0.0 6 344 4 235 335 0.0 6 332 2 0 0 0 0 1 236 303 0.0 6 300 3 237 351 0.0 6 345 4 1 1 238 271 0.0 6 265 6 239 232 0.0 6 227 5 240 247 0.0 6 246 0 1 241 280 0.0 6 277 3 242 336 0.0 6 333 3 243 379 0.0 6 377 2 244 171 0.0 6 168 2 0 0 0 0 1 245 168 0.0 6 167 1 246 153 0.0 6 151 1 1 247 185 0.0 6 183 2 248 235 0.0 6 234 1 249 288 0.0 6 287 1 250 276 0.0 6 276 251 213 0.0 6 213 252 239 0.0 6 239 253 233 0.0 6 232 1 254 230 0.0 6 226 4 255 340 0.0 6 337 3 256 130 0.0 6 128 2 257 202 0.0 6 201 1 258 154 0.0 6 153 1 259 130 0.0 6 128 1 1 260 141 0.0 6 140 1 261 137 0.0 6 136 1 262 106 0.0 6 103 3 263 109 0.0 6 108 1 264 90 0.0 6 90 265 130 0.0 6 128 0 2 266 177 0.0 6 174 3 267 142 0.0 6 141 1 268 72 0.0 6 72 269 88 0.0 6 86 2 270 92 0.0 6 90 1 0 1 271 111 0.0 6 110 1 272 120 0.0 6 120 273 165 0.0 6 164 1 274 159 0.0 6 157 2 275 221 0.0 6 221 276 224 0.0 6 224 277 766 0.0 6 760 6 278 885 0.0 6 872 12 0 1 279 11 0.0 6 11 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1560 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 2 191.9 1 2 64 2 0.0 6 2 72 7 0.0 6 7 73 9 0.0 6 9 74 9 0.0 6 9 75 38 0.0 6 38 76 18 0.0 6 18 77 11 0.0 6 11 78 37 0.0 6 37 79 44 0.0 6 44 80 8 0.0 6 8 81 5 0.0 6 5 82 19 0.0 6 19 83 44 0.0 6 43 1 84 5 0.0 6 5 85 1 0.0 6 1 86 6 0.0 6 6 87 12 0.0 6 12 88 1 0.0 6 1 89 1 0.0 6 1 90 1 0.0 6 1 91 3 0.0 6 3 93 1 0.0 6 1 94 2 0.0 6 2 95 3 0.0 6 3 96 4 0.0 6 4 97 3 0.0 6 3 98 2 0.0 6 2 99 2 0.0 6 2 100 7 0.0 6 7 101 8 0.0 6 7 1 102 45 0.0 6 45 103 41 0.0 6 41 104 5 0.0 6 5 105 9 0.0 6 9 107 3 0.0 6 3 108 7 0.0 6 7 109 2 0.0 6 2 110 4 0.0 6 4 111 5 0.0 6 5 113 1 0.0 6 1 114 3 0.0 6 3 118 2 0.0 6 2 119 15 0.0 6 15 120 2 0.0 6 2 121 23 0.0 6 23 122 121 0.0 6 121 123 76 0.0 6 76 124 7 0.0 6 7 125 1 0.0 6 1 126 3 0.0 6 3 127 7 0.0 6 7 128 1 0.0 6 1 129 7 0.0 6 7 130 16 0.0 6 16 132 1 0.0 6 1 133 4 0.0 6 4 134 15 0.0 6 15 135 20 0.0 6 20 136 44 0.0 6 44 137 22 0.0 6 22 138 5 0.0 6 5 139 20 0.0 6 20 140 20 0.0 6 20 141 1 0.0 6 1 142 3 0.0 6 3 143 1 0.0 6 1 145 1 0.0 6 1 146 1 0.0 6 0 1 147 1 0.0 6 1 148 1 0.0 6 1 150 2 0.0 6 2 153 1 0.0 6 1 155 14 0.0 6 14 156 33 0.0 6 33 157 6 0.0 6 6 158 7 0.0 6 7 160 4 0.0 6 4 162 5 0.0 6 5 163 6 0.0 6 6 165 5 0.0 6 5 166 14 0.0 6 14 167 8 0.0 6 8 168 4 0.0 6 4 169 5 0.0 6 5 170 12 0.0 6 12 175 1 0.0 6 1 176 8 0.0 6 8 177 9 0.0 6 9 180 1 0.0 6 1 181 8 0.0 6 8 182 3 0.0 6 3 183 6 0.0 6 6 184 21 0.0 6 21 185 10 0.0 6 10 186 50 0.0 6 50 187 102 0.0 6 102 188 7 0.0 6 7 189 6 0.0 6 6 190 3 0.0 6 3 191 3 0.0 6 3 192 7 0.0 6 6 0 0 0 0 0 1 193 1 0.0 6 1 195 2 0.0 6 2 196 11 0.0 6 11 197 23 0.0 6 23 198 4 0.0 6 4 201 2 0.0 6 2 202 2 0.0 6 2 203 1 0.0 6 1 204 1 0.0 6 1 205 2 0.0 6 2 206 6 0.0 6 6 207 21 0.0 6 21 208 13 0.0 6 13 209 1 0.0 6 1 210 1 0.0 6 1 214 4 0.0 6 4 215 17 0.0 6 17 217 2 0.0 6 2 219 1 0.0 6 1 221 3 0.0 6 3 222 3 0.0 6 3 225 3 0.0 6 3 226 9 0.0 6 9 227 1 0.0 6 1 228 1 0.0 6 1 230 5 0.0 6 5 231 23 0.0 6 23 232 26 0.0 6 25 1 234 1 0.0 6 1 235 1 0.0 6 1 236 5 0.0 6 5 237 9 0.0 6 9 244 1 0.0 6 1 247 1 0.0 6 1 248 2 0.0 6 2 249 4 0.0 6 4 250 14 0.0 6 14 251 7 0.0 6 7 254 1 0.0 6 1 255 7 0.0 6 7 256 1 0.0 6 1 257 1 0.0 6 1 260 1 0.0 6 1 262 4 0.0 6 4 263 8 0.0 6 8 264 1 0.0 6 1 265 10 0.0 6 10 266 19 0.0 6 19 267 1 0.0 6 1 269 1 0.0 6 1 272 1 0.0 6 1 273 7 0.0 6 7 274 1 0.0 6 1 275 1 0.0 6 1 278 1 0.0 6 1 288 2 0.0 6 2 289 1 0.0 6 1 290 1 0.0 6 1 291 4 0.0 6 4 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 47411 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 9 3 0.2 2 3 11 2 0.0 3 2 12 2 0.0 3 2 13 7 0.0 3 6 1 14 3 0.0 4 3 15 1 0.0 4 1 16 11 0.0 4 10 1 17 8 0.0 5 8 18 8 0.0 5 8 19 6 0.0 5 5 1 20 50 0.0 6 48 2 21 23 0.0 6 22 1 22 12 0.0 6 11 1 23 11 0.0 6 11 24 10 0.0 6 10 25 8 0.0 6 6 0 0 1 1 26 11 0.0 6 11 27 14 0.0 6 12 2 28 4 0.0 6 4 29 10 0.0 6 9 1 30 17 0.0 6 17 31 5 0.0 6 5 32 13 0.0 6 13 33 11 0.0 6 11 34 7 0.0 6 6 1 35 2 0.0 6 2 36 6 0.0 6 6 37 5 0.0 6 5 38 8 0.0 6 8 39 3 0.0 6 3 40 8 0.0 6 8 41 5 0.0 6 4 0 0 0 0 0 1 42 9 0.0 6 8 0 0 0 0 0 1 43 8 0.0 6 8 44 7 0.0 6 6 1 45 11 0.0 6 10 0 0 0 0 0 1 46 7 0.0 6 7 47 9 0.0 6 9 48 17 0.0 6 16 1 49 6 0.0 6 4 1 1 50 10 0.0 6 10 51 7 0.0 6 7 52 16 0.0 6 16 53 16 0.0 6 15 0 1 54 12 0.0 6 12 55 24 0.0 6 18 3 1 1 1 56 11 0.0 6 11 57 15 0.0 6 12 2 1 58 21 0.0 6 20 1 59 18 0.0 6 14 4 60 18 0.0 6 17 1 61 23 0.0 6 21 2 62 11 0.0 6 9 2 63 8 0.0 6 7 1 64 13 0.0 6 10 2 0 0 0 1 65 21 0.0 6 20 1 66 18 0.0 6 17 1 67 21 0.0 6 20 1 68 25 0.0 6 23 2 69 108 0.0 6 103 4 0 0 0 1 70 40 0.0 6 39 1 71 56 0.0 6 54 2 72 23 0.0 6 19 3 0 1 73 35 0.0 6 34 1 74 22 0.0 6 22 75 23 0.0 6 23 76 26 0.0 6 25 0 1 77 25 0.0 6 25 78 25 0.0 6 23 2 79 25 0.0 6 23 2 80 22 0.0 6 21 1 81 33 0.0 6 33 82 33 0.0 6 32 1 83 29 0.0 6 27 1 1 84 39 0.0 6 37 2 85 49 0.0 6 47 2 86 28 0.0 6 26 2 87 38 0.0 6 36 0 1 0 0 1 88 36 0.0 6 34 2 89 32 0.0 6 29 2 0 0 0 1 90 40 0.0 6 38 2 91 35 0.0 6 35 92 64 0.0 6 61 3 93 53 0.0 6 51 2 94 46 0.0 6 45 1 95 57 0.0 6 56 0 0 0 0 1 96 46 0.0 6 44 2 97 58 0.0 6 55 3 98 59 0.0 6 58 1 99 48 0.0 6 47 0 0 0 0 1 100 55 0.0 6 55 101 44 0.0 6 43 1 102 40 0.0 6 39 0 0 0 0 0 1 103 54 0.0 6 52 2 104 47 0.0 6 47 105 62 0.0 6 59 3 106 134 0.0 6 132 2 107 70 0.0 6 65 4 1 108 76 0.0 6 73 2 0 0 0 0 1 109 75 0.0 6 74 1 110 85 0.0 6 83 2 111 73 0.0 6 72 0 0 0 1 112 107 0.0 6 107 113 88 0.0 6 87 1 114 95 0.0 6 94 1 115 76 0.0 6 71 4 1 116 91 0.0 6 86 5 117 107 0.0 6 105 2 118 87 0.0 6 85 1 1 119 120 0.0 6 116 2 1 0 0 1 120 143 0.0 6 138 4 0 0 0 0 1 121 118 0.0 6 111 5 2 122 143 0.0 6 136 7 123 119 0.0 6 114 5 124 134 0.0 6 132 1 0 0 0 0 1 125 136 0.0 6 133 2 0 0 0 0 1 126 169 0.0 6 164 3 1 0 0 1 127 172 0.0 6 169 2 0 1 128 165 0.0 6 162 3 129 227 0.0 6 223 3 0 0 0 1 130 210 0.0 6 207 3 131 208 0.0 6 205 3 132 265 0.0 6 262 3 133 260 0.0 6 248 10 0 1 0 1 134 267 0.0 6 263 4 135 293 0.0 6 284 9 136 375 0.0 6 368 7 137 327 0.0 6 317 8 1 0 0 1 138 322 0.0 6 320 2 139 408 0.0 6 406 2 140 382 0.0 6 376 4 1 0 1 141 613 0.0 6 606 7 142 530 0.0 6 526 4 143 893 0.0 6 878 12 2 1 144 846 0.0 6 836 10 145 593 0.0 6 586 7 146 1226 0.0 6 1212 13 1 147 1147 0.0 6 1130 16 0 0 0 1 148 762 0.0 6 750 11 0 0 0 1 149 690 0.0 6 679 11 150 807 0.0 6 799 7 0 0 0 1 151 1366 0.0 6 1350 14 0 0 0 1 1 152 1040 0.0 6 1025 11 1 1 1 0 1 153 854 0.0 6 841 12 0 0 1 154 891 0.0 6 880 11 155 832 0.0 6 820 11 1 156 678 0.0 6 663 14 1 157 573 0.0 6 560 11 0 1 0 0 1 158 538 0.0 6 523 14 0 0 0 0 1 159 671 0.0 6 666 5 160 694 0.0 6 687 7 161 564 0.0 6 555 7 1 0 0 1 162 558 0.0 6 549 9 163 559 0.0 6 544 14 0 0 0 1 164 601 0.0 6 591 9 0 0 0 0 1 165 605 0.0 6 594 11 166 625 0.0 6 619 5 0 0 0 1 167 614 0.0 6 611 3 168 798 0.0 6 786 6 2 2 0 1 1 169 631 0.0 6 619 10 0 0 0 0 2 170 1139 0.0 6 1130 8 0 0 0 1 171 826 0.0 6 815 11 172 597 0.0 6 585 10 0 0 0 0 2 173 985 0.0 6 974 10 0 1 174 659 0.0 6 653 5 1 175 572 0.0 6 563 9 176 523 0.0 6 511 11 0 0 0 0 1 177 441 0.0 6 434 7 178 531 0.0 6 523 7 1 179 430 0.0 6 426 4 180 418 0.0 6 412 5 0 0 0 1 181 332 0.0 6 328 4 182 317 0.0 6 311 5 0 0 0 0 1 183 283 0.0 6 278 2 1 1 0 0 1 184 363 0.0 6 355 8 185 429 0.0 6 426 2 0 0 1 186 742 0.0 6 731 10 1 187 728 0.0 6 716 11 1 188 551 0.0 6 537 12 1 0 0 0 1 189 581 0.0 6 577 4 190 586 0.0 6 577 7 1 0 0 0 1 191 396 0.0 6 393 3 192 252 0.0 6 251 1 193 316 0.0 6 313 3 194 243 0.0 6 240 3 195 248 0.0 6 243 5 196 234 0.0 6 232 2 197 234 0.0 6 233 0 1 198 254 0.0 6 252 1 1 199 229 0.0 6 224 4 0 1 200 171 0.0 6 170 1 201 243 0.0 6 242 1 202 140 0.0 6 137 2 1 203 141 0.0 6 138 3 204 256 0.0 6 254 2 205 87 0.0 6 83 4 206 110 0.0 6 109 1 207 98 0.0 6 97 1 208 85 0.0 6 83 1 1 209 68 0.0 6 68 210 94 0.0 6 94 211 67 0.0 6 63 4 212 54 0.0 6 53 1 213 125 0.0 6 125 214 100 0.0 6 99 1 215 152 0.0 6 152 216 64 0.0 6 62 2 217 68 0.0 6 68 218 58 0.0 6 58 219 52 0.0 6 49 2 1 220 40 0.0 6 39 1 221 93 0.0 6 93 222 32 0.0 6 32 223 70 0.0 6 68 2 224 36 0.0 6 36 225 48 0.0 6 47 1 226 29 0.0 6 29 227 27 0.0 6 27 228 16 0.0 6 16 229 34 0.0 6 34 230 9 0.0 6 9 231 22 0.0 6 22 232 13 0.0 6 13 233 12 0.0 6 11 1 234 16 0.0 6 16 235 6 0.0 6 6 236 5 0.0 6 5 237 2 0.0 6 2 238 8 0.0 6 8 239 3 0.0 6 3 240 1 0.0 6 1 241 3 0.0 6 3 242 1 0.0 6 1 243 1 0.0 6 1 244 1 0.0 6 1 245 1 0.0 6 1 246 1 0.0 6 1 247 2 0.0 6 2 248 2 0.0 6 2 250 1 0.0 6 1 251 1 0.0 6 1 254 1 0.0 6 1 279 6 0.0 6 6 280 8 0.0 6 7 1 281 31 0.0 6 30 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1662 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 43 1 0.0 6 0 0 0 0 0 1 68 1 0.0 6 1 69 7 0.0 6 6 1 70 225 0.0 6 221 4 71 14 0.0 6 14 72 50 0.0 6 50 73 23 0.0 6 23 74 5 0.0 6 4 0 0 0 1 75 47 0.0 6 47 76 56 0.0 6 56 77 4 0.0 6 4 78 4 0.0 6 4 79 19 0.0 6 18 0 0 0 1 80 62 0.0 6 62 81 6 0.0 6 6 82 2 0.0 6 2 83 2 0.0 6 2 84 25 0.0 6 24 0 0 0 1 85 1 0.0 6 1 86 1 0.0 6 1 87 4 0.0 6 4 88 4 0.0 6 4 89 1 0.0 6 1 90 3 0.0 6 3 91 2 0.0 6 2 92 1 0.0 6 1 93 4 0.0 6 4 94 4 0.0 6 4 97 5 0.0 6 5 98 11 0.0 6 11 99 49 0.0 6 49 100 51 0.0 6 51 101 2 0.0 6 2 102 16 0.0 6 15 1 104 5 0.0 6 5 105 7 0.0 6 7 106 1 0.0 6 1 107 6 0.0 6 6 108 7 0.0 6 7 109 1 0.0 6 1 110 2 0.0 6 2 111 2 0.0 6 2 114 3 0.0 6 3 115 2 0.0 6 2 116 10 0.0 6 10 117 2 0.0 6 2 118 25 0.0 6 25 119 139 0.0 6 139 120 88 0.0 6 88 121 10 0.0 6 10 122 9 0.0 6 8 1 123 7 0.0 6 6 0 0 0 1 124 2 0.0 6 2 126 7 0.0 6 7 127 28 0.0 6 27 1 128 1 0.0 6 1 129 1 0.0 6 1 130 4 0.0 6 4 131 15 0.0 6 15 132 33 0.0 6 33 133 64 0.0 6 63 1 134 20 0.0 6 20 135 7 0.0 6 7 136 16 0.0 6 15 0 0 0 1 137 20 0.0 6 20 139 3 0.0 6 3 144 2 0.0 6 2 145 5 0.0 6 5 147 4 0.0 6 4 150 1 0.0 6 1 151 1 0.0 6 1 152 11 0.0 6 11 153 39 0.0 6 39 154 1 0.0 6 1 155 15 0.0 6 15 157 2 0.0 6 2 158 3 0.0 6 3 159 5 0.0 6 5 160 1 0.0 6 1 161 1 0.0 6 1 162 3 0.0 6 3 163 22 0.0 6 22 164 13 0.0 6 13 165 2 0.0 6 2 166 6 0.0 6 6 167 11 0.0 6 11 168 1 0.0 6 1 172 2 0.0 6 2 174 3 0.0 6 3 177 2 0.0 6 2 178 10 0.0 6 10 179 1 0.0 6 0 1 180 10 0.0 6 10 181 16 0.0 6 16 182 10 0.0 6 9 1 183 35 0.0 6 34 1 184 114 0.0 6 114 185 4 0.0 6 4 186 6 0.0 6 6 187 2 0.0 6 0 0 0 1 1 188 1 0.0 6 1 189 1 0.0 6 1 193 1 0.0 6 1 194 4 0.0 6 4 195 3 0.0 6 3 196 1 0.0 6 1 199 1 0.0 6 1 203 2 0.0 6 2 204 7 0.0 6 7 205 2 0.0 6 2 206 1 0.0 6 1 207 1 0.0 6 1 212 6 0.0 6 6 214 2 0.0 6 2 217 1 0.0 6 1 219 1 0.0 6 1 228 1 0.0 6 1 229 1 0.0 6 1 245 1 0.0 6 1 260 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_09_180_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_09_407_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_09_180_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_09_407_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_10_181_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_10_408_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_10_181_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_10_408_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_11_182_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_11_409_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_11_182_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_11_409_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_12_183_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_12_410_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_12_183_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_12_410_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_13_184_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_18-Rev_13_411_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_13_184_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_18-Rev_13_411_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_07_185_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_07_412_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_07_185_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_07_412_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_08_186_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_08_413_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_08_186_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_08_413_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_09_187_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_09_414_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_09_187_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_09_414_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_10_188_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_10_415_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_10_188_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_10_415_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_11_189_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_11_416_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_11_189_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_11_416_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_12_190_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_12_417_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_12_190_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_12_417_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_13_191_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_19-Rev_13_418_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_13_191_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_19-Rev_13_418_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_07_192_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_07_419_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_07_192_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_07_419_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.43 s (36 µs/read; 1.66 M reads/minute). === Summary === Total read pairs processed: 94,633 Read 1 with adapter: 94,619 (100.0%) Read 2 with adapter: 94,580 (99.9%) == Read fate breakdown == Pairs that were too short: 42 (0.0%) Pairs discarded as untrimmed: 67 (0.1%) Pairs written (passing filters): 94,524 (99.9%) Total basepairs processed: 39,552,959 bp Read 1: 21,752,663 bp Read 2: 17,800,296 bp Total written (filtered): 6,223,774 bp (15.7%) Read 1: 3,136,539 bp Read 2: 3,087,235 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 92477 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 1478.6 0 4 4 7 369.7 1 4 3 5 1 92.4 1 1 6 4 23.1 1 0 0 4 7 3 5.8 2 1 0 2 9 2 0.4 2 2 15 3 0.0 4 3 16 4 0.0 4 3 1 17 6 0.0 5 6 18 1 0.0 5 1 19 1 0.0 5 1 20 151 0.0 6 149 2 21 82 0.0 6 78 4 22 81 0.0 6 81 23 33 0.0 6 33 24 30 0.0 6 29 1 25 31 0.0 6 29 2 26 26 0.0 6 25 1 27 23 0.0 6 22 1 28 13 0.0 6 12 0 1 29 9 0.0 6 9 30 15 0.0 6 15 31 9 0.0 6 9 32 10 0.0 6 10 33 11 0.0 6 11 34 9 0.0 6 9 35 9 0.0 6 9 36 4 0.0 6 4 37 7 0.0 6 7 38 11 0.0 6 10 1 39 12 0.0 6 11 1 40 12 0.0 6 11 1 41 16 0.0 6 15 1 42 15 0.0 6 14 0 0 0 0 0 1 43 5 0.0 6 5 44 7 0.0 6 7 45 8 0.0 6 8 46 5 0.0 6 4 1 47 7 0.0 6 6 1 48 14 0.0 6 12 2 49 13 0.0 6 13 50 5 0.0 6 4 0 0 0 0 0 1 51 11 0.0 6 11 52 16 0.0 6 15 1 53 8 0.0 6 7 1 54 7 0.0 6 7 55 8 0.0 6 8 56 4 0.0 6 3 1 57 12 0.0 6 11 0 0 0 0 1 58 7 0.0 6 7 59 2 0.0 6 1 1 60 8 0.0 6 8 61 12 0.0 6 12 62 9 0.0 6 9 63 11 0.0 6 11 64 9 0.0 6 9 65 12 0.0 6 11 0 1 66 7 0.0 6 7 67 10 0.0 6 10 68 12 0.0 6 11 1 69 13 0.0 6 12 1 70 10 0.0 6 10 71 19 0.0 6 19 72 10 0.0 6 10 73 7 0.0 6 7 74 17 0.0 6 17 75 15 0.0 6 15 76 13 0.0 6 13 77 7 0.0 6 6 1 78 7 0.0 6 6 1 79 21 0.0 6 20 1 80 17 0.0 6 16 1 81 17 0.0 6 16 1 82 12 0.0 6 12 83 25 0.0 6 25 84 20 0.0 6 19 1 85 16 0.0 6 15 1 86 14 0.0 6 14 87 14 0.0 6 13 1 88 5 0.0 6 4 1 89 13 0.0 6 13 90 11 0.0 6 10 1 91 6 0.0 6 6 92 13 0.0 6 13 93 15 0.0 6 15 94 11 0.0 6 11 95 14 0.0 6 13 1 96 13 0.0 6 13 97 17 0.0 6 17 98 14 0.0 6 13 0 0 0 1 99 14 0.0 6 13 1 100 19 0.0 6 17 2 101 14 0.0 6 14 102 20 0.0 6 20 103 30 0.0 6 28 2 104 32 0.0 6 31 1 105 28 0.0 6 26 2 106 25 0.0 6 23 2 107 27 0.0 6 25 2 108 25 0.0 6 23 2 109 34 0.0 6 33 0 0 0 0 0 1 110 32 0.0 6 32 111 40 0.0 6 39 1 112 43 0.0 6 42 1 113 31 0.0 6 30 0 1 114 34 0.0 6 32 1 0 0 0 1 115 34 0.0 6 34 116 37 0.0 6 36 1 117 36 0.0 6 35 0 0 0 1 118 35 0.0 6 34 1 119 46 0.0 6 45 1 120 40 0.0 6 40 121 46 0.0 6 45 0 0 0 0 1 122 45 0.0 6 45 123 53 0.0 6 51 1 0 0 0 0 1 124 39 0.0 6 39 125 55 0.0 6 55 126 31 0.0 6 30 1 127 40 0.0 6 39 1 128 54 0.0 6 54 129 55 0.0 6 55 130 54 0.0 6 52 2 131 49 0.0 6 49 132 67 0.0 6 67 133 71 0.0 6 69 1 0 0 0 0 1 134 57 0.0 6 56 1 135 56 0.0 6 56 136 65 0.0 6 64 1 137 61 0.0 6 61 138 82 0.0 6 82 139 79 0.0 6 79 140 106 0.0 6 103 3 141 93 0.0 6 93 142 146 0.0 6 143 3 143 346 0.0 6 346 144 809 0.0 6 808 1 145 1025 0.0 6 1024 0 0 1 146 798 0.0 6 797 1 147 622 0.0 6 620 2 148 963 0.0 6 961 2 149 843 0.0 6 840 3 150 782 0.0 6 782 151 849 0.0 6 849 152 801 0.0 6 797 3 1 153 744 0.0 6 742 2 154 676 0.0 6 675 1 155 606 0.0 6 605 1 156 546 0.0 6 542 3 0 0 1 157 565 0.0 6 564 1 158 542 0.0 6 541 0 0 0 0 0 1 159 485 0.0 6 485 160 613 0.0 6 611 1 0 0 0 1 161 557 0.0 6 555 2 162 635 0.0 6 634 1 163 685 0.0 6 685 164 591 0.0 6 589 2 165 555 0.0 6 553 2 166 697 0.0 6 696 1 167 516 0.0 6 512 4 168 606 0.0 6 603 2 0 0 1 169 638 0.0 6 635 3 170 561 0.0 6 560 1 171 623 0.0 6 620 3 172 596 0.0 6 594 2 173 604 0.0 6 604 174 555 0.0 6 551 3 1 175 777 0.0 6 773 4 176 711 0.0 6 710 1 177 526 0.0 6 526 178 669 0.0 6 668 1 179 613 0.0 6 609 4 180 636 0.0 6 635 1 181 1726 0.0 6 1725 1 182 881 0.0 6 880 1 183 659 0.0 6 657 2 184 689 0.0 6 689 185 633 0.0 6 631 2 186 689 0.0 6 687 2 187 710 0.0 6 706 3 1 188 669 0.0 6 669 189 664 0.0 6 662 1 0 0 1 190 786 0.0 6 785 1 191 694 0.0 6 692 2 192 783 0.0 6 777 6 193 785 0.0 6 784 0 0 0 0 1 194 797 0.0 6 794 2 0 0 0 1 195 1042 0.0 6 1039 3 196 1226 0.0 6 1221 5 197 1141 0.0 6 1138 2 1 198 1266 0.0 6 1258 7 0 1 199 1280 0.0 6 1276 4 200 953 0.0 6 952 1 201 954 0.0 6 951 2 0 1 202 954 0.0 6 951 3 203 951 0.0 6 945 5 0 1 204 1027 0.0 6 1023 3 0 0 0 0 1 205 1415 0.0 6 1409 6 206 1187 0.0 6 1183 4 207 1071 0.0 6 1067 3 0 0 1 208 1138 0.0 6 1134 3 1 209 868 0.0 6 867 1 210 951 0.0 6 949 2 211 1008 0.0 6 1008 212 1055 0.0 6 1048 6 1 213 1106 0.0 6 1102 4 214 1161 0.0 6 1160 1 215 1296 0.0 6 1291 5 216 1057 0.0 6 1052 4 1 217 1075 0.0 6 1073 1 1 218 1102 0.0 6 1097 3 1 0 1 219 1035 0.0 6 1030 5 220 812 0.0 6 807 5 221 779 0.0 6 775 4 222 786 0.0 6 785 1 223 836 0.0 6 830 6 224 857 0.0 6 855 1 1 225 970 0.0 6 965 5 226 962 0.0 6 955 7 227 739 0.0 6 736 2 0 0 0 1 228 705 0.0 6 702 3 229 720 0.0 6 717 3 230 673 0.0 6 673 231 711 0.0 6 706 5 232 667 0.0 6 664 3 233 708 0.0 6 705 2 0 0 1 234 618 0.0 6 616 2 235 505 0.0 6 502 3 236 573 0.0 6 573 237 498 0.0 6 497 1 238 483 0.0 6 483 239 486 0.0 6 483 3 240 474 0.0 6 473 1 241 508 0.0 6 506 2 242 468 0.0 6 465 3 243 378 0.0 6 376 2 244 357 0.0 6 356 1 245 358 0.0 6 358 246 296 0.0 6 295 0 0 0 1 247 264 0.0 6 262 2 248 270 0.0 6 269 1 249 284 0.0 6 282 1 0 0 1 250 288 0.0 6 285 2 0 0 0 1 251 234 0.0 6 233 1 252 218 0.0 6 218 253 200 0.0 6 200 254 205 0.0 6 202 3 255 189 0.0 6 189 256 168 0.0 6 168 257 209 0.0 6 204 5 258 199 0.0 6 197 2 259 227 0.0 6 226 1 260 206 0.0 6 205 1 261 119 0.0 6 119 262 142 0.0 6 139 3 263 174 0.0 6 171 2 1 264 144 0.0 6 142 2 265 140 0.0 6 140 266 118 0.0 6 116 1 0 1 267 159 0.0 6 158 1 268 118 0.0 6 118 269 147 0.0 6 145 2 270 87 0.0 6 87 271 94 0.0 6 93 1 272 116 0.0 6 116 273 120 0.0 6 120 274 147 0.0 6 145 2 275 133 0.0 6 131 2 276 223 0.0 6 219 4 277 395 0.0 6 389 6 278 710 0.0 6 705 5 279 2259 0.0 6 2220 38 1 280 21 0.0 6 16 5 281 2 0.0 6 2 283 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2142 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 1 0.0 6 1 63 148 0.0 6 145 3 64 1 0.0 6 1 65 3 0.0 6 3 71 2 0.0 6 2 72 19 0.0 6 19 73 12 0.0 6 12 74 31 0.0 6 31 75 14 0.0 6 14 76 20 0.0 6 20 77 16 0.0 6 16 78 15 0.0 6 15 79 15 0.0 6 15 80 14 0.0 6 14 81 25 0.0 6 25 82 17 0.0 6 17 83 19 0.0 6 19 84 19 0.0 6 19 85 16 0.0 6 16 86 6 0.0 6 6 87 10 0.0 6 10 88 15 0.0 6 15 89 5 0.0 6 5 90 13 0.0 6 13 91 17 0.0 6 17 92 13 0.0 6 13 93 8 0.0 6 8 94 8 0.0 6 8 95 34 0.0 6 34 96 11 0.0 6 11 97 11 0.0 6 11 98 10 0.0 6 10 99 15 0.0 6 15 100 10 0.0 6 10 101 11 0.0 6 11 102 10 0.0 6 10 103 17 0.0 6 17 104 16 0.0 6 16 105 15 0.0 6 15 106 7 0.0 6 7 107 14 0.0 6 14 108 11 0.0 6 10 1 109 22 0.0 6 22 110 44 0.0 6 44 111 15 0.0 6 15 112 8 0.0 6 8 113 18 0.0 6 18 114 12 0.0 6 12 115 17 0.0 6 17 116 13 0.0 6 13 117 13 0.0 6 13 118 13 0.0 6 13 119 13 0.0 6 13 120 10 0.0 6 10 121 8 0.0 6 8 122 9 0.0 6 9 123 12 0.0 6 12 124 11 0.0 6 11 125 19 0.0 6 19 126 10 0.0 6 10 127 17 0.0 6 17 128 18 0.0 6 18 129 10 0.0 6 10 130 8 0.0 6 8 131 12 0.0 6 12 132 12 0.0 6 12 133 13 0.0 6 13 134 25 0.0 6 25 135 8 0.0 6 8 136 14 0.0 6 14 137 7 0.0 6 7 138 12 0.0 6 12 139 19 0.0 6 19 140 9 0.0 6 9 141 15 0.0 6 15 142 13 0.0 6 13 143 14 0.0 6 14 144 13 0.0 6 13 145 6 0.0 6 6 146 13 0.0 6 13 147 14 0.0 6 14 148 11 0.0 6 11 149 14 0.0 6 14 150 10 0.0 6 10 151 12 0.0 6 12 152 19 0.0 6 19 153 10 0.0 6 10 154 11 0.0 6 11 155 18 0.0 6 18 156 13 0.0 6 13 157 11 0.0 6 11 158 17 0.0 6 17 159 11 0.0 6 11 160 22 0.0 6 22 161 8 0.0 6 8 162 21 0.0 6 21 163 13 0.0 6 13 164 19 0.0 6 19 165 17 0.0 6 17 166 10 0.0 6 10 167 11 0.0 6 11 168 10 0.0 6 10 169 11 0.0 6 11 170 15 0.0 6 15 171 11 0.0 6 11 172 2 0.0 6 2 173 18 0.0 6 18 174 12 0.0 6 12 175 16 0.0 6 16 176 18 0.0 6 18 177 12 0.0 6 12 178 11 0.0 6 11 179 7 0.0 6 7 180 6 0.0 6 6 181 8 0.0 6 8 182 14 0.0 6 14 183 6 0.0 6 6 184 12 0.0 6 12 185 10 0.0 6 10 186 11 0.0 6 11 187 8 0.0 6 8 188 6 0.0 6 6 189 13 0.0 6 13 190 4 0.0 6 4 191 14 0.0 6 14 192 8 0.0 6 8 193 12 0.0 6 12 194 12 0.0 6 12 195 9 0.0 6 9 196 13 0.0 6 13 197 7 0.0 6 7 198 1 0.0 6 1 199 12 0.0 6 12 200 5 0.0 6 5 201 9 0.0 6 9 202 12 0.0 6 12 203 17 0.0 6 17 204 5 0.0 6 5 205 6 0.0 6 6 206 4 0.0 6 4 207 6 0.0 6 6 208 7 0.0 6 7 209 11 0.0 6 11 210 8 0.0 6 8 211 6 0.0 6 6 212 7 0.0 6 7 213 10 0.0 6 10 214 3 0.0 6 3 215 5 0.0 6 5 216 4 0.0 6 4 217 3 0.0 6 3 218 7 0.0 6 7 219 7 0.0 6 6 0 0 0 0 0 1 220 4 0.0 6 4 221 13 0.0 6 13 222 3 0.0 6 3 223 4 0.0 6 4 224 4 0.0 6 4 225 5 0.0 6 5 226 4 0.0 6 4 227 9 0.0 6 9 228 7 0.0 6 7 229 7 0.0 6 7 230 3 0.0 6 3 231 3 0.0 6 3 233 8 0.0 6 8 234 1 0.0 6 1 235 5 0.0 6 5 236 5 0.0 6 5 237 3 0.0 6 3 238 2 0.0 6 2 239 1 0.0 6 1 240 1 0.0 6 1 241 2 0.0 6 2 242 3 0.0 6 3 243 1 0.0 6 1 244 2 0.0 6 2 245 8 0.0 6 8 246 1 0.0 6 1 247 4 0.0 6 4 248 3 0.0 6 3 249 2 0.0 6 1 1 250 2 0.0 6 2 251 1 0.0 6 1 252 2 0.0 6 2 253 1 0.0 6 1 254 3 0.0 6 3 255 1 0.0 6 1 256 1 0.0 6 1 257 2 0.0 6 2 258 1 0.0 6 1 259 3 0.0 6 3 260 2 0.0 6 2 261 3 0.0 6 3 263 1 0.0 6 1 264 1 0.0 6 1 265 1 0.0 6 1 267 1 0.0 6 1 268 1 0.0 6 1 269 2 0.0 6 2 272 1 0.0 6 1 275 2 0.0 6 2 276 1 0.0 6 1 277 1 0.0 6 1 279 1 0.0 6 1 280 1 0.0 6 1 282 1 0.0 6 1 289 1 0.0 6 1 293 1 0.0 6 1 296 1 0.0 6 1 298 1 0.0 6 1 302 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 92478 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 1478.6 0 0 3 5 1 92.4 1 1 8 1 1.4 2 0 1 10 3 0.1 3 2 1 11 4 0.0 3 4 13 10 0.0 3 8 2 14 9 0.0 4 8 1 15 1 0.0 4 1 16 18 0.0 4 15 3 17 31 0.0 5 27 4 18 28 0.0 5 27 0 1 19 17 0.0 5 17 20 115 0.0 6 112 3 21 55 0.0 6 51 4 22 28 0.0 6 27 1 23 33 0.0 6 31 2 24 22 0.0 6 19 2 1 25 28 0.0 6 26 1 1 26 18 0.0 6 17 1 27 20 0.0 6 17 2 0 0 0 0 1 28 25 0.0 6 23 2 29 18 0.0 6 18 30 19 0.0 6 18 1 31 19 0.0 6 19 32 9 0.0 6 9 33 15 0.0 6 13 2 34 12 0.0 6 10 2 35 13 0.0 6 11 2 36 12 0.0 6 11 1 37 12 0.0 6 11 1 38 13 0.0 6 12 1 39 13 0.0 6 12 1 40 15 0.0 6 14 1 41 20 0.0 6 19 1 42 12 0.0 6 12 43 10 0.0 6 10 44 18 0.0 6 16 1 0 1 45 18 0.0 6 17 1 46 20 0.0 6 18 1 0 1 47 15 0.0 6 14 0 1 48 21 0.0 6 19 2 49 19 0.0 6 18 1 50 20 0.0 6 19 0 0 0 1 51 24 0.0 6 24 52 24 0.0 6 20 3 1 53 25 0.0 6 25 54 21 0.0 6 17 4 55 24 0.0 6 24 56 20 0.0 6 20 57 20 0.0 6 17 2 0 1 58 21 0.0 6 19 2 59 30 0.0 6 26 3 1 60 30 0.0 6 27 3 61 23 0.0 6 20 3 62 29 0.0 6 28 1 63 34 0.0 6 33 1 64 49 0.0 6 48 1 65 41 0.0 6 39 2 66 63 0.0 6 59 3 1 67 178 0.0 6 171 5 1 0 1 68 75 0.0 6 71 2 1 0 0 0 1 69 95 0.0 6 91 2 1 0 0 0 1 70 51 0.0 6 50 1 71 59 0.0 6 56 2 1 72 39 0.0 6 37 1 1 73 42 0.0 6 40 2 74 46 0.0 6 45 1 75 64 0.0 6 61 3 76 36 0.0 6 34 1 0 0 0 1 77 40 0.0 6 36 3 0 0 1 78 46 0.0 6 43 3 79 76 0.0 6 76 80 49 0.0 6 46 2 1 81 59 0.0 6 57 2 82 70 0.0 6 66 4 83 86 0.0 6 83 1 0 1 0 0 1 84 63 0.0 6 62 1 85 53 0.0 6 51 1 0 1 86 57 0.0 6 54 3 87 65 0.0 6 61 4 88 49 0.0 6 46 3 89 51 0.0 6 51 90 75 0.0 6 70 5 91 86 0.0 6 83 3 92 74 0.0 6 69 5 93 87 0.0 6 80 6 1 94 80 0.0 6 77 3 95 82 0.0 6 79 3 96 83 0.0 6 82 1 97 92 0.0 6 88 2 0 0 1 0 1 98 115 0.0 6 113 2 99 99 0.0 6 94 1 0 1 0 2 1 100 105 0.0 6 103 2 101 113 0.0 6 108 5 102 97 0.0 6 95 1 1 103 132 0.0 6 127 5 104 237 0.0 6 232 3 0 0 1 0 1 105 113 0.0 6 112 1 106 126 0.0 6 125 1 107 136 0.0 6 129 6 1 108 148 0.0 6 146 2 109 134 0.0 6 131 3 110 149 0.0 6 144 3 0 1 0 0 1 111 147 0.0 6 144 2 1 112 228 0.0 6 221 6 0 1 113 158 0.0 6 153 4 0 0 0 0 1 114 156 0.0 6 153 3 115 203 0.0 6 197 6 116 179 0.0 6 174 4 0 0 0 1 117 233 0.0 6 229 2 0 1 1 118 229 0.0 6 226 3 119 213 0.0 6 210 3 120 216 0.0 6 208 5 1 0 1 1 121 245 0.0 6 241 2 0 0 0 2 122 251 0.0 6 243 6 0 0 1 0 1 123 244 0.0 6 240 3 0 1 124 317 0.0 6 310 5 1 0 0 0 1 125 363 0.0 6 356 5 1 1 126 357 0.0 6 350 6 0 0 1 127 403 0.0 6 401 1 1 128 451 0.0 6 441 9 0 0 0 0 1 129 443 0.0 6 432 8 1 0 0 2 130 517 0.0 6 509 5 1 1 0 0 1 131 623 0.0 6 610 11 0 0 0 2 132 537 0.0 6 526 7 1 1 2 133 579 0.0 6 571 7 0 0 0 0 1 134 735 0.0 6 721 13 0 0 1 135 614 0.0 6 609 3 1 0 0 0 1 136 637 0.0 6 628 8 0 1 137 614 0.0 6 602 9 0 1 0 1 1 138 669 0.0 6 659 9 0 0 0 1 139 724 0.0 6 714 7 1 1 0 1 140 993 0.0 6 978 13 1 0 0 0 1 141 1397 0.0 6 1389 7 1 142 1801 0.0 6 1791 6 0 1 1 1 1 143 1369 0.0 6 1361 7 0 0 0 1 144 1379 0.0 6 1364 11 2 1 0 1 145 1758 0.0 6 1743 13 1 1 146 1630 0.0 6 1616 13 0 0 0 0 1 147 1589 0.0 6 1573 13 1 0 1 1 148 1981 0.0 6 1961 16 2 0 0 0 2 149 1943 0.0 6 1927 13 2 0 0 1 150 2342 0.0 6 2322 14 1 1 1 1 2 151 1928 0.0 6 1909 14 1 0 1 1 2 152 2074 0.0 6 2049 21 0 1 2 1 153 2036 0.0 6 2019 14 1 0 1 1 154 1589 0.0 6 1578 6 1 0 2 1 1 155 1754 0.0 6 1736 15 1 1 0 0 1 156 1568 0.0 6 1559 6 0 0 0 1 2 157 1789 0.0 6 1782 4 1 1 0 0 1 158 1803 0.0 6 1789 12 0 0 0 0 2 159 1554 0.0 6 1537 12 2 1 1 0 1 160 1591 0.0 6 1571 13 2 0 4 1 161 1477 0.0 6 1470 4 1 1 0 0 1 162 1324 0.0 6 1319 4 0 0 0 0 1 163 1354 0.0 6 1338 13 0 0 2 1 164 1302 0.0 6 1293 6 1 0 2 165 1207 0.0 6 1199 5 1 0 1 0 1 166 1436 0.0 6 1420 12 3 0 0 1 167 1257 0.0 6 1248 8 0 0 1 168 1542 0.0 6 1537 4 0 0 0 1 169 1292 0.0 6 1289 3 170 1314 0.0 6 1303 10 0 0 1 171 1393 0.0 6 1385 8 172 1489 0.0 6 1482 4 0 1 1 1 173 1400 0.0 6 1391 9 174 1200 0.0 6 1192 6 0 1 0 1 175 1247 0.0 6 1242 4 0 0 0 1 176 1322 0.0 6 1312 9 0 0 0 0 1 177 1105 0.0 6 1093 9 0 1 0 0 2 178 1132 0.0 6 1126 6 179 800 0.0 6 792 8 180 716 0.0 6 714 2 181 694 0.0 6 689 5 182 677 0.0 6 671 5 1 183 942 0.0 6 937 5 184 1200 0.0 6 1190 9 1 185 979 0.0 6 974 5 186 741 0.0 6 737 4 187 721 0.0 6 715 4 1 0 0 0 1 188 629 0.0 6 627 2 189 602 0.0 6 597 2 1 1 0 1 190 572 0.0 6 566 5 0 1 191 601 0.0 6 599 2 192 439 0.0 6 434 4 0 0 1 193 423 0.0 6 417 6 194 390 0.0 6 387 2 1 195 345 0.0 6 342 2 1 196 501 0.0 6 495 6 197 348 0.0 6 346 2 198 354 0.0 6 349 5 199 362 0.0 6 358 4 200 259 0.0 6 257 2 201 285 0.0 6 284 1 202 219 0.0 6 218 1 203 180 0.0 6 178 2 204 198 0.0 6 194 4 205 151 0.0 6 151 206 147 0.0 6 147 207 116 0.0 6 115 1 208 116 0.0 6 116 209 109 0.0 6 105 3 0 0 1 210 86 0.0 6 83 3 211 85 0.0 6 82 3 212 116 0.0 6 114 1 0 1 213 88 0.0 6 85 3 214 167 0.0 6 163 4 215 88 0.0 6 84 4 216 91 0.0 6 89 2 217 61 0.0 6 61 218 59 0.0 6 59 219 66 0.0 6 65 1 220 23 0.0 6 23 221 67 0.0 6 66 1 222 60 0.0 6 59 1 223 82 0.0 6 82 224 42 0.0 6 41 1 225 36 0.0 6 36 226 26 0.0 6 26 227 24 0.0 6 24 228 20 0.0 6 19 1 229 34 0.0 6 34 230 15 0.0 6 14 1 231 26 0.0 6 26 232 30 0.0 6 29 1 233 24 0.0 6 23 1 234 11 0.0 6 11 235 7 0.0 6 7 236 1 0.0 6 1 237 3 0.0 6 3 238 2 0.0 6 2 239 8 0.0 6 8 240 12 0.0 6 12 241 11 0.0 6 11 242 10 0.0 6 10 243 8 0.0 6 8 244 5 0.0 6 5 245 12 0.0 6 12 246 7 0.0 6 6 1 247 2 0.0 6 2 248 8 0.0 6 7 1 249 2 0.0 6 2 250 2 0.0 6 2 251 7 0.0 6 7 252 2 0.0 6 2 275 1 0.0 6 1 277 1 0.0 6 1 278 9 0.0 6 8 1 279 65 0.0 6 65 280 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2102 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 49 1 0.0 6 0 1 52 3 0.0 6 3 53 1 0.0 6 1 55 1 0.0 6 1 56 2 0.0 6 2 58 3 0.0 6 3 69 1 0.0 6 1 70 32 0.0 6 32 71 5 0.0 6 4 1 72 19 0.0 6 19 73 38 0.0 6 38 74 32 0.0 6 32 75 20 0.0 6 20 76 19 0.0 6 19 77 30 0.0 6 30 78 24 0.0 6 24 79 25 0.0 6 25 80 29 0.0 6 28 1 81 22 0.0 6 22 82 21 0.0 6 21 83 17 0.0 6 17 84 18 0.0 6 18 85 19 0.0 6 19 86 26 0.0 6 26 87 16 0.0 6 16 88 9 0.0 6 9 89 25 0.0 6 25 90 13 0.0 6 13 91 21 0.0 6 21 92 19 0.0 6 19 93 11 0.0 6 11 94 15 0.0 6 15 95 14 0.0 6 14 96 14 0.0 6 14 97 22 0.0 6 22 98 13 0.0 6 13 99 13 0.0 6 13 100 15 0.0 6 15 101 15 0.0 6 13 2 102 17 0.0 6 17 103 12 0.0 6 12 104 17 0.0 6 16 1 105 19 0.0 6 19 106 9 0.0 6 8 0 0 0 1 107 14 0.0 6 13 0 0 1 108 14 0.0 6 14 109 28 0.0 6 28 110 52 0.0 6 52 111 12 0.0 6 12 112 12 0.0 6 12 113 9 0.0 6 9 114 5 0.0 6 5 115 20 0.0 6 20 116 14 0.0 6 14 117 17 0.0 6 17 118 16 0.0 6 16 119 18 0.0 6 18 120 11 0.0 6 11 121 9 0.0 6 9 122 14 0.0 6 14 123 13 0.0 6 13 124 14 0.0 6 14 125 19 0.0 6 19 126 9 0.0 6 9 127 17 0.0 6 17 128 24 0.0 6 24 129 15 0.0 6 15 130 12 0.0 6 12 131 16 0.0 6 16 132 20 0.0 6 19 1 133 22 0.0 6 21 0 0 0 1 134 23 0.0 6 23 135 9 0.0 6 9 136 15 0.0 6 15 137 18 0.0 6 18 138 11 0.0 6 11 139 20 0.0 6 20 140 17 0.0 6 17 141 16 0.0 6 16 142 31 0.0 6 31 143 16 0.0 6 16 144 15 0.0 6 15 145 14 0.0 6 14 146 13 0.0 6 13 147 19 0.0 6 19 148 16 0.0 6 16 149 13 0.0 6 13 150 18 0.0 6 18 151 13 0.0 6 12 0 0 0 1 152 16 0.0 6 16 153 12 0.0 6 12 154 16 0.0 6 16 155 27 0.0 6 27 156 17 0.0 6 16 1 157 15 0.0 6 15 158 17 0.0 6 17 159 14 0.0 6 14 160 18 0.0 6 18 161 10 0.0 6 10 162 24 0.0 6 24 163 23 0.0 6 23 164 30 0.0 6 29 1 165 23 0.0 6 22 0 0 0 1 166 15 0.0 6 14 1 167 13 0.0 6 13 168 10 0.0 6 10 169 10 0.0 6 10 170 16 0.0 6 16 171 13 0.0 6 12 0 0 0 1 172 9 0.0 6 9 173 13 0.0 6 13 174 14 0.0 6 14 175 15 0.0 6 15 176 9 0.0 6 9 177 4 0.0 6 4 178 13 0.0 6 13 179 15 0.0 6 14 0 0 1 180 10 0.0 6 10 181 8 0.0 6 8 182 11 0.0 6 11 183 7 0.0 6 7 184 10 0.0 6 10 185 5 0.0 6 5 186 10 0.0 6 10 187 6 0.0 6 6 188 8 0.0 6 8 189 9 0.0 6 9 190 9 0.0 6 9 191 8 0.0 6 8 192 13 0.0 6 13 193 6 0.0 6 6 194 7 0.0 6 7 195 1 0.0 6 1 196 5 0.0 6 5 197 9 0.0 6 9 198 4 0.0 6 4 199 3 0.0 6 3 200 5 0.0 6 5 201 1 0.0 6 1 202 7 0.0 6 7 203 6 0.0 6 6 204 6 0.0 6 6 205 5 0.0 6 5 206 3 0.0 6 3 207 3 0.0 6 3 209 2 0.0 6 2 210 1 0.0 6 1 211 2 0.0 6 2 212 4 0.0 6 4 213 2 0.0 6 2 215 3 0.0 6 3 219 2 0.0 6 2 220 1 0.0 6 1 221 2 0.0 6 2 222 3 0.0 6 3 228 1 0.0 6 1 231 1 0.0 6 1 234 1 0.0 6 1 235 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_08_193_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_08_420_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_08_193_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_08_420_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.33 s (30 µs/read; 1.97 M reads/minute). === Summary === Total read pairs processed: 141,933 Read 1 with adapter: 141,911 (100.0%) Read 2 with adapter: 141,933 (100.0%) == Read fate breakdown == Pairs that were too short: 18 (0.0%) Pairs discarded as untrimmed: 22 (0.0%) Pairs written (passing filters): 141,893 (100.0%) Total basepairs processed: 64,545,030 bp Read 1: 35,608,544 bp Read 2: 28,936,486 bp Total written (filtered): 9,183,858 bp (14.2%) Read 1: 4,688,291 bp Read 2: 4,495,567 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 139466 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 6 2217.7 0 4 2 4 8 554.4 1 2 6 6 3 34.7 1 0 0 3 7 7 8.7 2 4 0 3 8 4 2.2 2 4 9 2 0.5 2 2 11 1 0.0 3 1 12 1 0.0 3 1 13 2 0.0 3 1 1 14 1 0.0 4 1 15 7 0.0 4 5 2 16 6 0.0 4 4 2 17 5 0.0 5 4 1 18 1 0.0 5 0 0 1 19 5 0.0 5 4 1 20 143 0.0 6 139 3 1 21 82 0.0 6 81 1 22 83 0.0 6 81 2 23 45 0.0 6 44 1 24 42 0.0 6 40 2 25 41 0.0 6 41 26 38 0.0 6 38 27 12 0.0 6 11 1 28 13 0.0 6 12 1 29 17 0.0 6 17 30 7 0.0 6 6 1 31 11 0.0 6 10 1 32 16 0.0 6 15 0 1 33 7 0.0 6 6 1 34 12 0.0 6 11 0 1 35 6 0.0 6 5 1 36 10 0.0 6 9 1 37 5 0.0 6 5 38 14 0.0 6 14 39 9 0.0 6 8 1 40 11 0.0 6 11 41 11 0.0 6 11 42 14 0.0 6 13 1 43 7 0.0 6 7 44 9 0.0 6 8 1 45 11 0.0 6 11 46 8 0.0 6 8 47 15 0.0 6 13 2 48 7 0.0 6 7 49 8 0.0 6 6 1 0 0 1 50 7 0.0 6 7 51 7 0.0 6 6 1 52 1 0.0 6 1 53 9 0.0 6 9 54 7 0.0 6 7 55 8 0.0 6 8 56 7 0.0 6 7 57 11 0.0 6 10 1 58 13 0.0 6 12 0 1 59 7 0.0 6 7 60 5 0.0 6 5 61 9 0.0 6 9 62 7 0.0 6 7 63 11 0.0 6 11 64 15 0.0 6 14 1 65 11 0.0 6 10 1 66 11 0.0 6 11 67 10 0.0 6 10 68 12 0.0 6 12 69 13 0.0 6 12 0 1 70 8 0.0 6 8 71 16 0.0 6 15 0 0 0 0 1 72 10 0.0 6 10 73 8 0.0 6 8 74 9 0.0 6 9 75 14 0.0 6 14 76 11 0.0 6 11 77 14 0.0 6 14 78 11 0.0 6 11 79 8 0.0 6 8 80 10 0.0 6 10 81 13 0.0 6 12 0 1 82 15 0.0 6 14 1 83 17 0.0 6 17 84 19 0.0 6 18 1 85 19 0.0 6 19 86 21 0.0 6 21 87 22 0.0 6 20 2 88 7 0.0 6 7 89 15 0.0 6 14 1 90 19 0.0 6 19 91 7 0.0 6 7 92 16 0.0 6 14 2 93 9 0.0 6 8 1 94 13 0.0 6 11 2 95 16 0.0 6 16 96 11 0.0 6 10 1 97 16 0.0 6 15 1 98 16 0.0 6 16 99 15 0.0 6 15 100 19 0.0 6 17 2 101 31 0.0 6 30 1 102 26 0.0 6 23 3 103 31 0.0 6 30 1 104 25 0.0 6 24 1 105 34 0.0 6 33 1 106 22 0.0 6 21 1 107 28 0.0 6 28 108 39 0.0 6 37 2 109 32 0.0 6 32 110 38 0.0 6 37 1 111 38 0.0 6 38 112 52 0.0 6 50 2 113 44 0.0 6 43 0 0 0 1 114 45 0.0 6 43 2 115 58 0.0 6 58 116 41 0.0 6 39 1 1 117 42 0.0 6 42 118 54 0.0 6 52 2 119 52 0.0 6 50 2 120 38 0.0 6 35 3 121 51 0.0 6 49 1 1 122 57 0.0 6 54 3 123 70 0.0 6 68 2 124 56 0.0 6 53 3 125 60 0.0 6 59 0 1 126 66 0.0 6 64 0 1 1 127 56 0.0 6 55 1 128 69 0.0 6 67 2 129 67 0.0 6 65 2 130 55 0.0 6 54 1 131 80 0.0 6 80 132 72 0.0 6 71 1 133 68 0.0 6 68 134 94 0.0 6 93 1 135 77 0.0 6 76 0 1 136 106 0.0 6 106 137 101 0.0 6 99 2 138 95 0.0 6 95 139 99 0.0 6 98 0 1 140 120 0.0 6 119 1 141 167 0.0 6 166 1 142 378 0.0 6 378 143 785 0.0 6 783 1 0 1 144 869 0.0 6 866 3 145 1007 0.0 6 1006 1 146 1169 0.0 6 1165 4 147 1516 0.0 6 1516 148 1445 0.0 6 1444 1 149 1578 0.0 6 1575 3 150 1524 0.0 6 1523 1 151 1499 0.0 6 1495 3 0 0 1 152 2013 0.0 6 2010 3 153 1149 0.0 6 1147 2 154 850 0.0 6 848 2 155 929 0.0 6 928 1 156 814 0.0 6 811 3 157 887 0.0 6 885 2 158 756 0.0 6 754 2 159 764 0.0 6 760 3 0 0 0 0 1 160 928 0.0 6 927 1 161 834 0.0 6 829 5 162 999 0.0 6 998 1 163 946 0.0 6 944 1 1 164 864 0.0 6 864 165 725 0.0 6 723 2 166 544 0.0 6 544 167 721 0.0 6 720 1 168 679 0.0 6 679 169 706 0.0 6 702 4 170 640 0.0 6 636 3 1 171 681 0.0 6 675 6 172 807 0.0 6 807 173 596 0.0 6 594 2 174 660 0.0 6 658 2 175 691 0.0 6 689 2 176 592 0.0 6 590 2 177 523 0.0 6 523 178 468 0.0 6 465 1 1 0 0 0 1 179 559 0.0 6 554 5 180 996 0.0 6 994 2 181 569 0.0 6 567 2 182 492 0.0 6 490 1 1 183 546 0.0 6 542 4 184 585 0.0 6 583 2 185 633 0.0 6 633 186 593 0.0 6 590 3 187 537 0.0 6 535 2 188 508 0.0 6 503 5 189 454 0.0 6 454 190 511 0.0 6 509 2 191 540 0.0 6 536 4 192 591 0.0 6 582 8 1 193 673 0.0 6 667 6 194 710 0.0 6 700 10 195 920 0.0 6 912 7 0 1 196 805 0.0 6 804 1 197 802 0.0 6 796 5 1 198 1078 0.0 6 1069 8 0 0 0 0 1 199 722 0.0 6 719 3 200 556 0.0 6 553 2 0 0 0 1 201 633 0.0 6 628 5 202 497 0.0 6 495 1 1 203 697 0.0 6 690 7 204 888 0.0 6 885 3 205 810 0.0 6 806 3 0 0 0 1 206 800 0.0 6 796 4 207 745 0.0 6 736 8 1 208 877 0.0 6 867 9 1 209 1034 0.0 6 1020 13 0 0 1 210 867 0.0 6 845 21 0 0 0 0 1 211 1307 0.0 6 1285 21 0 1 212 906 0.0 6 892 13 1 213 938 0.0 6 926 12 214 1348 0.0 6 1329 19 215 932 0.0 6 926 6 216 1276 0.0 6 1257 17 0 1 0 0 1 217 720 0.0 6 715 4 0 0 1 218 722 0.0 6 711 8 2 1 219 653 0.0 6 649 4 220 611 0.0 6 603 8 221 511 0.0 6 506 5 222 616 0.0 6 611 5 223 513 0.0 6 502 11 224 666 0.0 6 658 8 225 674 0.0 6 664 9 1 226 1071 0.0 6 1058 13 227 716 0.0 6 703 13 228 697 0.0 6 690 7 229 727 0.0 6 716 10 1 230 686 0.0 6 674 12 231 670 0.0 6 657 13 232 534 0.0 6 529 5 233 471 0.0 6 461 10 234 453 0.0 6 448 4 0 1 235 423 0.0 6 417 5 1 236 432 0.0 6 426 6 237 494 0.0 6 487 7 238 367 0.0 6 362 5 239 511 0.0 6 504 7 240 1068 0.0 6 1057 10 1 241 358 0.0 6 351 6 1 242 469 0.0 6 462 7 243 338 0.0 6 333 5 244 276 0.0 6 272 4 245 222 0.0 6 219 3 246 390 0.0 6 381 9 247 545 0.0 6 533 11 1 248 400 0.0 6 388 11 1 249 410 0.0 6 401 9 250 667 0.0 6 648 15 3 1 251 465 0.0 6 448 16 0 1 252 1834 0.0 6 1778 55 1 253 553 0.0 6 539 12 1 1 254 438 0.0 6 427 11 255 564 0.0 6 551 13 256 785 0.0 6 761 23 0 1 257 861 0.0 6 845 16 258 777 0.0 6 733 43 1 259 1353 0.0 6 1322 27 4 260 573 0.0 6 538 33 1 0 1 261 863 0.0 6 840 21 2 262 450 0.0 6 431 18 1 263 992 0.0 6 972 18 1 0 1 264 395 0.0 6 384 9 2 265 314 0.0 6 302 12 266 679 0.0 6 656 23 267 863 0.0 6 846 17 268 317 0.0 6 303 13 1 269 854 0.0 6 838 16 270 368 0.0 6 356 12 271 589 0.0 6 572 16 1 272 364 0.0 6 350 13 1 273 518 0.0 6 508 10 274 469 0.0 6 453 14 2 275 819 0.0 6 776 43 276 2645 0.0 6 2581 63 0 1 277 2019 0.0 6 1964 52 1 2 278 1695 0.0 6 1513 177 4 0 1 279 30643 0.0 6 30095 537 9 2 280 11 0.0 6 9 1 1 281 3 0.0 6 3 282 2 0.0 6 0 1 0 0 1 283 1 0.0 6 1 284 1 0.0 6 1 285 3 0.0 6 3 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2445 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 3 0.0 6 1 2 63 431 0.0 6 420 11 64 1 0.0 6 1 65 2 0.0 6 2 66 1 0.0 6 0 0 0 0 0 0 1 69 1 0.0 6 1 71 10 0.0 6 10 72 18 0.0 6 17 1 73 21 0.0 6 21 74 28 0.0 6 28 75 35 0.0 6 35 76 39 0.0 6 39 77 47 0.0 6 47 78 52 0.0 6 51 1 79 48 0.0 6 48 80 44 0.0 6 43 1 81 53 0.0 6 53 82 92 0.0 6 91 1 83 30 0.0 6 30 84 26 0.0 6 26 85 30 0.0 6 29 0 0 0 0 0 1 86 23 0.0 6 23 87 21 0.0 6 21 88 25 0.0 6 25 89 10 0.0 6 10 90 38 0.0 6 37 1 91 55 0.0 6 55 92 32 0.0 6 32 93 29 0.0 6 29 94 22 0.0 6 22 95 18 0.0 6 18 96 13 0.0 6 13 97 23 0.0 6 23 98 9 0.0 6 9 99 23 0.0 6 23 100 21 0.0 6 21 101 18 0.0 6 18 102 25 0.0 6 25 103 16 0.0 6 16 104 11 0.0 6 11 105 14 0.0 6 14 106 8 0.0 6 8 107 22 0.0 6 22 108 8 0.0 6 8 109 22 0.0 6 22 110 28 0.0 6 28 111 21 0.0 6 21 112 12 0.0 6 12 113 7 0.0 6 7 114 20 0.0 6 20 115 17 0.0 6 17 116 15 0.0 6 15 117 11 0.0 6 11 118 8 0.0 6 8 119 8 0.0 6 8 120 5 0.0 6 5 121 10 0.0 6 10 122 8 0.0 6 8 123 11 0.0 6 11 124 6 0.0 6 6 125 14 0.0 6 14 126 10 0.0 6 10 127 15 0.0 6 15 128 14 0.0 6 14 129 8 0.0 6 8 130 10 0.0 6 10 131 13 0.0 6 13 132 4 0.0 6 4 133 5 0.0 6 5 134 16 0.0 6 16 135 13 0.0 6 13 136 6 0.0 6 6 137 11 0.0 6 11 138 11 0.0 6 11 139 9 0.0 6 9 140 17 0.0 6 17 141 8 0.0 6 8 142 11 0.0 6 11 143 7 0.0 6 7 144 7 0.0 6 7 145 9 0.0 6 9 146 8 0.0 6 8 147 8 0.0 6 8 148 9 0.0 6 9 149 14 0.0 6 14 150 7 0.0 6 7 151 5 0.0 6 5 152 6 0.0 6 6 153 7 0.0 6 7 154 10 0.0 6 10 155 8 0.0 6 8 156 7 0.0 6 7 157 11 0.0 6 11 158 6 0.0 6 6 159 6 0.0 6 6 160 10 0.0 6 10 161 7 0.0 6 7 162 8 0.0 6 8 163 13 0.0 6 13 164 14 0.0 6 14 165 6 0.0 6 6 166 6 0.0 6 6 167 9 0.0 6 9 168 1 0.0 6 1 169 6 0.0 6 6 170 6 0.0 6 6 171 22 0.0 6 22 172 18 0.0 6 18 173 11 0.0 6 11 174 9 0.0 6 9 175 6 0.0 6 6 176 3 0.0 6 3 177 5 0.0 6 5 178 5 0.0 6 5 179 6 0.0 6 6 180 6 0.0 6 6 181 10 0.0 6 10 182 6 0.0 6 6 183 5 0.0 6 5 184 6 0.0 6 6 185 5 0.0 6 5 186 3 0.0 6 3 187 7 0.0 6 7 188 6 0.0 6 6 189 2 0.0 6 2 190 9 0.0 6 9 191 7 0.0 6 7 192 5 0.0 6 5 193 3 0.0 6 3 194 9 0.0 6 9 195 2 0.0 6 2 196 4 0.0 6 4 197 5 0.0 6 5 198 5 0.0 6 5 199 3 0.0 6 3 200 3 0.0 6 3 201 3 0.0 6 3 202 5 0.0 6 5 203 7 0.0 6 7 204 2 0.0 6 2 205 1 0.0 6 1 206 7 0.0 6 7 207 2 0.0 6 2 208 3 0.0 6 3 209 3 0.0 6 3 210 6 0.0 6 6 211 3 0.0 6 3 212 1 0.0 6 1 213 3 0.0 6 3 214 5 0.0 6 5 215 1 0.0 6 1 216 2 0.0 6 2 217 1 0.0 6 1 218 2 0.0 6 2 219 1 0.0 6 1 220 2 0.0 6 2 221 1 0.0 6 1 222 2 0.0 6 2 223 2 0.0 6 2 224 1 0.0 6 1 225 2 0.0 6 2 226 4 0.0 6 4 227 1 0.0 6 1 228 3 0.0 6 3 229 5 0.0 6 5 234 4 0.0 6 4 235 3 0.0 6 3 236 4 0.0 6 4 237 4 0.0 6 4 238 1 0.0 6 1 239 1 0.0 6 1 240 1 0.0 6 1 242 3 0.0 6 3 243 2 0.0 6 2 244 3 0.0 6 3 245 1 0.0 6 1 247 2 0.0 6 2 248 1 0.0 6 1 249 2 0.0 6 2 250 2 0.0 6 2 252 1 0.0 6 1 253 3 0.0 6 3 258 1 0.0 6 1 264 1 0.0 6 1 268 2 0.0 6 2 269 1 0.0 6 1 272 1 0.0 6 1 277 2 0.0 6 2 284 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 139536 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 34.7 1 0 1 7 1 8.7 2 1 8 1 2.2 2 0 1 9 1 0.5 2 0 1 10 2 0.1 3 1 1 11 3 0.0 3 2 1 12 9 0.0 3 9 13 10 0.0 3 7 3 14 17 0.0 4 16 1 15 2 0.0 4 1 1 16 11 0.0 4 7 4 17 27 0.0 5 26 1 18 30 0.0 5 25 2 3 19 9 0.0 5 7 2 20 113 0.0 6 110 3 21 81 0.0 6 76 5 22 52 0.0 6 52 23 37 0.0 6 35 2 24 23 0.0 6 22 1 25 35 0.0 6 32 3 26 23 0.0 6 22 1 27 25 0.0 6 22 2 1 28 25 0.0 6 22 3 29 14 0.0 6 14 30 9 0.0 6 4 4 1 31 18 0.0 6 15 3 32 12 0.0 6 11 1 33 10 0.0 6 9 0 1 34 10 0.0 6 10 35 14 0.0 6 13 0 0 0 0 1 36 8 0.0 6 8 37 15 0.0 6 13 2 38 12 0.0 6 11 0 1 39 16 0.0 6 16 40 19 0.0 6 18 1 41 14 0.0 6 14 42 9 0.0 6 8 1 43 14 0.0 6 14 44 22 0.0 6 22 45 22 0.0 6 20 1 1 46 26 0.0 6 25 1 47 31 0.0 6 27 3 1 48 28 0.0 6 26 2 49 30 0.0 6 26 4 50 25 0.0 6 21 3 1 51 23 0.0 6 22 1 52 27 0.0 6 23 3 1 53 27 0.0 6 22 5 54 30 0.0 6 28 2 55 31 0.0 6 28 3 56 33 0.0 6 31 1 0 1 57 29 0.0 6 26 2 1 58 26 0.0 6 23 2 0 0 0 1 59 34 0.0 6 30 4 60 31 0.0 6 28 2 0 0 0 1 61 27 0.0 6 27 62 23 0.0 6 22 1 63 24 0.0 6 22 1 0 0 0 0 1 64 38 0.0 6 37 1 65 37 0.0 6 35 1 0 1 66 40 0.0 6 35 4 1 67 78 0.0 6 74 3 0 1 68 255 0.0 6 245 10 69 68 0.0 6 65 3 70 111 0.0 6 108 2 0 1 71 49 0.0 6 47 2 72 100 0.0 6 96 3 0 0 1 73 47 0.0 6 46 1 74 29 0.0 6 28 1 75 53 0.0 6 51 2 76 52 0.0 6 49 3 77 45 0.0 6 43 1 1 78 52 0.0 6 51 1 79 53 0.0 6 52 1 80 62 0.0 6 62 81 40 0.0 6 36 4 82 58 0.0 6 53 5 83 117 0.0 6 112 4 0 0 0 1 84 129 0.0 6 123 5 0 1 85 71 0.0 6 67 4 86 56 0.0 6 54 2 87 70 0.0 6 58 10 1 0 0 0 1 88 57 0.0 6 56 1 89 53 0.0 6 50 3 90 77 0.0 6 76 1 91 84 0.0 6 80 4 92 81 0.0 6 76 3 1 0 0 0 1 93 76 0.0 6 72 3 1 94 151 0.0 6 142 8 1 95 76 0.0 6 74 2 96 102 0.0 6 99 3 97 91 0.0 6 89 1 0 0 0 1 98 97 0.0 6 95 2 99 106 0.0 6 102 4 100 72 0.0 6 69 3 101 100 0.0 6 96 4 102 124 0.0 6 113 7 2 0 1 0 1 103 113 0.0 6 110 3 104 164 0.0 6 160 2 0 0 2 105 226 0.0 6 216 8 2 106 127 0.0 6 120 6 1 107 145 0.0 6 136 9 108 105 0.0 6 100 4 1 109 119 0.0 6 115 3 1 110 134 0.0 6 131 2 1 111 161 0.0 6 158 3 112 148 0.0 6 142 5 1 113 166 0.0 6 164 2 114 165 0.0 6 160 5 115 151 0.0 6 149 2 116 178 0.0 6 175 3 117 160 0.0 6 158 2 118 192 0.0 6 188 3 1 119 247 0.0 6 238 7 0 1 1 120 203 0.0 6 197 6 121 188 0.0 6 180 7 0 1 122 202 0.0 6 198 4 123 201 0.0 6 199 2 124 279 0.0 6 274 5 125 299 0.0 6 295 3 0 0 1 126 345 0.0 6 340 5 127 346 0.0 6 339 7 128 367 0.0 6 363 3 0 0 0 1 129 405 0.0 6 399 4 0 1 1 130 478 0.0 6 471 7 131 650 0.0 6 639 11 132 563 0.0 6 554 7 0 1 0 0 1 133 488 0.0 6 481 7 134 561 0.0 6 552 7 2 135 689 0.0 6 681 7 0 0 1 136 567 0.0 6 557 8 0 1 0 1 137 616 0.0 6 607 9 138 573 0.0 6 569 4 139 678 0.0 6 664 11 0 2 0 0 1 140 1071 0.0 6 1063 7 0 0 0 1 141 1238 0.0 6 1224 14 142 1532 0.0 6 1517 12 1 1 0 1 143 1689 0.0 6 1680 8 0 1 144 1803 0.0 6 1783 19 0 1 145 2037 0.0 6 2025 11 1 146 1904 0.0 6 1895 8 1 147 2036 0.0 6 2025 10 0 0 0 0 1 148 2109 0.0 6 2095 13 0 1 149 2278 0.0 6 2256 20 0 0 2 150 2706 0.0 6 2681 23 0 0 0 2 151 2539 0.0 6 2513 22 1 1 1 0 1 152 1756 0.0 6 1734 20 1 0 1 153 2056 0.0 6 2046 8 1 0 1 154 1930 0.0 6 1914 15 0 0 0 1 155 1499 0.0 6 1485 12 0 0 0 2 156 1743 0.0 6 1724 17 1 0 1 157 1572 0.0 6 1553 16 0 1 2 158 1798 0.0 6 1780 16 1 0 1 159 1622 0.0 6 1608 10 3 1 160 1673 0.0 6 1658 14 0 0 0 1 161 1569 0.0 6 1557 12 162 1288 0.0 6 1279 8 0 0 0 0 1 163 1307 0.0 6 1291 16 164 1249 0.0 6 1235 13 0 0 0 1 165 1357 0.0 6 1325 31 1 166 1445 0.0 6 1425 18 1 0 1 167 1394 0.0 6 1374 19 0 0 0 0 1 168 1541 0.0 6 1519 19 1 1 1 169 1489 0.0 6 1467 20 1 0 1 170 1336 0.0 6 1315 18 3 171 1268 0.0 6 1251 15 0 0 0 1 1 172 1223 0.0 6 1206 12 2 0 0 0 3 173 1408 0.0 6 1388 17 1 1 1 174 1334 0.0 6 1319 12 0 1 1 0 1 175 1477 0.0 6 1457 19 0 0 0 0 1 176 1291 0.0 6 1272 19 177 1586 0.0 6 1562 22 1 1 178 1122 0.0 6 1098 23 1 179 1237 0.0 6 1209 27 0 1 180 1164 0.0 6 1140 23 1 181 1323 0.0 6 1290 31 0 1 0 1 182 856 0.0 6 847 8 0 0 1 183 1087 0.0 6 1062 23 0 0 1 1 184 1183 0.0 6 1166 16 1 185 1179 0.0 6 1141 33 2 1 2 186 1630 0.0 6 1605 23 1 1 187 997 0.0 6 971 25 0 0 1 188 1284 0.0 6 1251 31 1 0 1 189 1415 0.0 6 1373 35 4 0 3 190 1781 0.0 6 1733 47 0 0 1 191 1732 0.0 6 1692 37 1 0 2 192 1102 0.0 6 1071 28 2 0 1 193 1485 0.0 6 1439 44 2 194 1203 0.0 6 1166 34 1 1 0 0 1 195 1118 0.0 6 1073 41 2 0 1 0 1 196 2300 0.0 6 2245 52 2 1 197 871 0.0 6 841 30 198 1893 0.0 6 1847 44 1 0 1 199 564 0.0 6 551 12 1 200 963 0.0 6 923 40 201 1295 0.0 6 1247 45 1 1 1 202 990 0.0 6 961 28 0 1 203 829 0.0 6 807 21 1 204 963 0.0 6 937 26 205 885 0.0 6 856 28 1 206 825 0.0 6 802 22 1 207 869 0.0 6 835 33 1 208 2097 0.0 6 2037 58 1 1 209 218 0.0 6 209 6 2 0 0 1 210 808 0.0 6 779 27 2 211 1570 0.0 6 1520 47 0 0 3 212 1061 0.0 6 1036 24 0 0 1 213 419 0.0 6 398 21 214 653 0.0 6 626 25 2 215 812 0.0 6 782 28 2 216 826 0.0 6 799 24 1 1 1 217 494 0.0 6 481 13 218 462 0.0 6 442 17 2 0 1 219 365 0.0 6 361 4 220 207 0.0 6 198 8 0 1 221 478 0.0 6 466 11 1 222 629 0.0 6 612 16 0 0 1 223 457 0.0 6 438 18 0 0 1 224 260 0.0 6 254 4 1 1 225 245 0.0 6 232 13 226 704 0.0 6 673 28 0 0 2 1 227 1848 0.0 6 1804 42 0 0 0 1 1 228 971 0.0 6 935 33 2 1 229 919 0.0 6 872 45 0 2 230 3450 0.0 6 3361 87 1 0 1 231 529 0.0 6 497 30 2 232 1272 0.0 6 1238 33 1 233 151 0.0 6 137 12 2 234 583 0.0 6 560 21 1 0 0 0 1 235 313 0.0 6 306 6 0 0 1 236 248 0.0 6 231 17 237 267 0.0 6 258 8 0 1 238 244 0.0 6 238 5 1 239 48 0.0 6 44 4 240 96 0.0 6 88 8 241 101 0.0 6 98 3 242 83 0.0 6 81 2 243 36 0.0 6 33 3 244 51 0.0 6 45 6 245 193 0.0 6 191 1 0 0 1 246 94 0.0 6 86 7 0 1 247 88 0.0 6 85 2 1 248 47 0.0 6 45 2 249 200 0.0 6 189 10 1 250 38 0.0 6 36 2 251 86 0.0 6 85 1 252 85 0.0 6 82 2 0 1 253 75 0.0 6 71 4 254 214 0.0 6 212 2 255 50 0.0 6 49 1 256 33 0.0 6 33 257 3 0.0 6 2 0 0 0 1 258 3 0.0 6 3 259 3 0.0 6 3 263 2 0.0 6 2 266 1 0.0 6 1 276 1 0.0 6 1 277 2 0.0 6 2 278 6 0.0 6 6 279 48 0.0 6 45 2 1 280 254 0.0 6 245 9 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2397 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 6 101 34.7 1 0 0 101 54 1 0.0 6 1 55 12 0.0 6 11 1 56 11 0.0 6 10 1 57 1 0.0 6 1 58 1 0.0 6 0 1 59 8 0.0 6 7 1 62 7 0.0 6 7 63 2 0.0 6 2 64 11 0.0 6 11 70 7 0.0 6 7 71 13 0.0 6 12 1 72 36 0.0 6 36 73 31 0.0 6 31 74 30 0.0 6 30 75 36 0.0 6 36 76 62 0.0 6 62 77 58 0.0 6 58 78 51 0.0 6 51 79 64 0.0 6 64 80 55 0.0 6 55 81 82 0.0 6 80 1 0 0 1 82 52 0.0 6 52 83 25 0.0 6 24 0 0 1 84 42 0.0 6 42 85 37 0.0 6 36 0 0 0 1 86 37 0.0 6 36 1 87 23 0.0 6 23 88 21 0.0 6 21 89 38 0.0 6 38 90 28 0.0 6 28 91 71 0.0 6 70 1 92 34 0.0 6 34 93 26 0.0 6 26 94 34 0.0 6 33 0 0 0 1 95 23 0.0 6 23 96 20 0.0 6 20 97 18 0.0 6 18 98 22 0.0 6 21 1 99 22 0.0 6 22 100 23 0.0 6 23 101 26 0.0 6 25 1 102 17 0.0 6 17 103 25 0.0 6 25 104 43 0.0 6 43 105 20 0.0 6 20 106 17 0.0 6 17 107 18 0.0 6 18 108 16 0.0 6 16 109 47 0.0 6 46 1 110 14 0.0 6 13 0 0 0 1 111 8 0.0 6 8 112 16 0.0 6 16 113 15 0.0 6 15 114 15 0.0 6 15 115 11 0.0 6 10 1 116 17 0.0 6 17 117 12 0.0 6 12 118 9 0.0 6 9 119 20 0.0 6 20 120 13 0.0 6 13 121 9 0.0 6 9 122 13 0.0 6 13 123 7 0.0 6 7 124 12 0.0 6 12 125 11 0.0 6 11 126 13 0.0 6 13 127 21 0.0 6 21 128 30 0.0 6 30 129 9 0.0 6 8 0 0 0 1 130 12 0.0 6 12 131 9 0.0 6 9 132 27 0.0 6 27 133 21 0.0 6 20 0 0 0 1 134 19 0.0 6 19 135 8 0.0 6 8 136 12 0.0 6 12 137 9 0.0 6 9 138 11 0.0 6 11 139 21 0.0 6 21 140 17 0.0 6 16 1 141 7 0.0 6 7 142 12 0.0 6 12 143 4 0.0 6 4 144 15 0.0 6 15 145 9 0.0 6 9 146 11 0.0 6 11 147 7 0.0 6 7 148 12 0.0 6 12 149 14 0.0 6 13 0 0 1 150 9 0.0 6 9 151 5 0.0 6 5 152 10 0.0 6 10 153 6 0.0 6 5 1 154 8 0.0 6 8 155 10 0.0 6 10 156 5 0.0 6 5 157 9 0.0 6 9 158 9 0.0 6 9 159 6 0.0 6 6 160 13 0.0 6 13 161 6 0.0 6 6 162 7 0.0 6 7 163 7 0.0 6 7 164 9 0.0 6 9 165 5 0.0 6 5 166 11 0.0 6 11 167 6 0.0 6 6 168 12 0.0 6 12 169 6 0.0 6 6 170 10 0.0 6 10 171 20 0.0 6 19 1 172 8 0.0 6 8 173 2 0.0 6 2 174 4 0.0 6 4 175 9 0.0 6 9 176 3 0.0 6 3 177 4 0.0 6 4 178 10 0.0 6 10 179 10 0.0 6 10 180 7 0.0 6 7 181 8 0.0 6 8 182 4 0.0 6 4 183 6 0.0 6 6 184 7 0.0 6 7 185 7 0.0 6 7 186 5 0.0 6 5 187 4 0.0 6 4 188 3 0.0 6 3 189 6 0.0 6 6 190 7 0.0 6 7 191 1 0.0 6 1 192 4 0.0 6 4 193 3 0.0 6 3 194 7 0.0 6 7 195 4 0.0 6 4 196 2 0.0 6 2 197 4 0.0 6 4 198 5 0.0 6 5 199 2 0.0 6 2 200 1 0.0 6 1 201 3 0.0 6 3 202 2 0.0 6 2 207 1 0.0 6 1 208 2 0.0 6 2 209 1 0.0 6 1 211 1 0.0 6 1 212 3 0.0 6 3 213 1 0.0 6 1 214 1 0.0 6 1 215 2 0.0 6 2 216 1 0.0 6 1 217 1 0.0 6 1 219 1 0.0 6 1 227 1 0.0 6 1 234 1 0.0 6 1 240 1 0.0 6 1 256 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_09_194_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_09_421_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_09_194_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_09_421_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.31 s (59 µs/read; 1.01 M reads/minute). === Summary === Total read pairs processed: 72,859 Read 1 with adapter: 72,855 (100.0%) Read 2 with adapter: 72,825 (100.0%) == Read fate breakdown == Pairs that were too short: 4 (0.0%) Pairs discarded as untrimmed: 38 (0.1%) Pairs written (passing filters): 72,817 (99.9%) Total basepairs processed: 35,297,340 bp Read 1: 19,913,667 bp Read 2: 15,383,673 bp Total written (filtered): 4,475,862 bp (12.7%) Read 1: 2,348,551 bp Read 2: 2,127,311 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 72527 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 4 284.6 1 0 4 5 1 71.2 1 1 6 1 17.8 1 0 0 1 7 1 4.4 2 1 8 1 1.1 2 1 9 1 0.3 2 1 11 1 0.0 3 1 15 1 0.0 4 1 16 2 0.0 4 2 17 2 0.0 5 2 20 29 0.0 6 28 1 21 17 0.0 6 16 1 22 7 0.0 6 7 23 2 0.0 6 1 1 24 6 0.0 6 5 1 25 13 0.0 6 13 26 7 0.0 6 6 1 27 7 0.0 6 7 28 3 0.0 6 3 30 7 0.0 6 7 32 5 0.0 6 5 33 4 0.0 6 4 34 5 0.0 6 4 1 35 2 0.0 6 1 1 36 3 0.0 6 3 37 1 0.0 6 0 1 38 4 0.0 6 3 0 1 39 3 0.0 6 3 40 3 0.0 6 3 41 5 0.0 6 5 42 3 0.0 6 3 43 3 0.0 6 3 44 3 0.0 6 3 45 1 0.0 6 1 46 3 0.0 6 2 1 47 1 0.0 6 1 48 3 0.0 6 3 49 3 0.0 6 1 1 1 50 6 0.0 6 6 51 3 0.0 6 3 52 2 0.0 6 2 53 7 0.0 6 6 1 54 1 0.0 6 1 56 5 0.0 6 5 57 2 0.0 6 2 58 3 0.0 6 3 59 1 0.0 6 1 60 3 0.0 6 3 61 4 0.0 6 3 0 1 62 5 0.0 6 5 63 2 0.0 6 2 64 2 0.0 6 2 65 6 0.0 6 6 66 4 0.0 6 2 2 67 2 0.0 6 2 68 5 0.0 6 5 69 2 0.0 6 2 70 2 0.0 6 2 71 4 0.0 6 3 1 72 5 0.0 6 5 73 2 0.0 6 2 74 5 0.0 6 5 75 2 0.0 6 1 1 76 2 0.0 6 2 77 3 0.0 6 3 78 4 0.0 6 4 79 6 0.0 6 5 0 1 80 5 0.0 6 4 1 81 4 0.0 6 3 0 0 0 0 0 1 82 1 0.0 6 1 83 5 0.0 6 5 84 4 0.0 6 4 85 2 0.0 6 2 86 13 0.0 6 12 1 87 3 0.0 6 3 88 5 0.0 6 5 89 7 0.0 6 7 90 6 0.0 6 6 91 2 0.0 6 1 1 92 9 0.0 6 8 1 93 14 0.0 6 13 0 1 94 1 0.0 6 1 95 3 0.0 6 2 1 96 1 0.0 6 1 97 6 0.0 6 5 1 98 8 0.0 6 8 99 4 0.0 6 3 1 100 10 0.0 6 9 1 101 11 0.0 6 10 1 102 14 0.0 6 11 3 103 7 0.0 6 7 104 12 0.0 6 11 1 105 15 0.0 6 12 2 0 0 0 1 106 7 0.0 6 7 107 10 0.0 6 9 1 108 24 0.0 6 23 1 109 5 0.0 6 5 110 14 0.0 6 14 111 15 0.0 6 14 1 112 21 0.0 6 21 113 21 0.0 6 21 114 16 0.0 6 16 115 19 0.0 6 19 116 12 0.0 6 12 117 16 0.0 6 15 0 0 0 0 1 118 7 0.0 6 7 119 18 0.0 6 17 1 120 8 0.0 6 7 1 121 15 0.0 6 14 1 122 24 0.0 6 24 123 24 0.0 6 24 124 20 0.0 6 19 1 125 15 0.0 6 14 1 126 16 0.0 6 15 1 127 12 0.0 6 12 128 26 0.0 6 26 129 25 0.0 6 24 0 1 130 23 0.0 6 23 131 18 0.0 6 18 132 23 0.0 6 21 1 0 0 0 1 133 20 0.0 6 20 134 26 0.0 6 26 135 22 0.0 6 22 136 23 0.0 6 22 1 137 28 0.0 6 28 138 30 0.0 6 30 139 35 0.0 6 35 140 126 0.0 6 124 2 141 181 0.0 6 181 142 204 0.0 6 202 2 143 204 0.0 6 201 3 144 209 0.0 6 207 2 145 239 0.0 6 239 146 203 0.0 6 201 2 147 249 0.0 6 249 148 228 0.0 6 224 4 149 298 0.0 6 297 1 150 322 0.0 6 320 1 1 151 225 0.0 6 224 1 152 120 0.0 6 120 153 136 0.0 6 136 154 208 0.0 6 207 1 155 139 0.0 6 138 1 156 115 0.0 6 115 157 211 0.0 6 209 1 1 158 142 0.0 6 140 1 0 0 1 159 117 0.0 6 117 160 191 0.0 6 190 1 161 245 0.0 6 242 3 162 147 0.0 6 146 1 163 184 0.0 6 183 1 164 172 0.0 6 172 165 149 0.0 6 147 2 166 103 0.0 6 101 2 167 82 0.0 6 80 2 168 120 0.0 6 119 1 169 193 0.0 6 193 170 135 0.0 6 131 3 1 171 201 0.0 6 200 1 172 154 0.0 6 153 1 173 165 0.0 6 164 1 174 168 0.0 6 166 2 175 195 0.0 6 191 4 176 144 0.0 6 141 2 0 0 1 177 181 0.0 6 181 178 323 0.0 6 321 2 179 239 0.0 6 236 3 180 149 0.0 6 149 181 136 0.0 6 133 3 182 203 0.0 6 202 1 183 173 0.0 6 170 2 1 184 192 0.0 6 189 2 1 185 227 0.0 6 224 3 186 212 0.0 6 209 3 187 203 0.0 6 200 3 188 230 0.0 6 225 5 189 268 0.0 6 260 8 190 187 0.0 6 184 3 191 278 0.0 6 271 6 1 192 279 0.0 6 276 3 193 327 0.0 6 322 5 194 339 0.0 6 332 6 1 195 395 0.0 6 388 6 0 0 1 196 394 0.0 6 385 9 197 326 0.0 6 317 8 1 198 450 0.0 6 447 2 1 199 353 0.0 6 347 5 1 200 282 0.0 6 278 4 201 337 0.0 6 332 5 202 408 0.0 6 403 5 203 437 0.0 6 432 5 204 363 0.0 6 354 9 205 414 0.0 6 406 8 206 391 0.0 6 383 8 207 433 0.0 6 429 4 208 555 0.0 6 545 10 209 434 0.0 6 426 8 210 318 0.0 6 309 9 211 264 0.0 6 259 5 212 262 0.0 6 259 3 213 318 0.0 6 311 5 0 2 214 369 0.0 6 362 5 1 1 215 376 0.0 6 370 6 216 333 0.0 6 330 3 217 480 0.0 6 474 6 218 353 0.0 6 349 3 0 1 219 312 0.0 6 303 8 0 0 1 220 284 0.0 6 276 8 221 242 0.0 6 239 3 222 405 0.0 6 397 7 1 223 185 0.0 6 181 4 224 175 0.0 6 171 3 1 225 301 0.0 6 296 4 1 226 655 0.0 6 647 8 227 353 0.0 6 344 8 0 0 0 1 228 336 0.0 6 329 6 1 229 311 0.0 6 307 4 230 323 0.0 6 320 3 231 258 0.0 6 252 6 232 243 0.0 6 238 5 233 234 0.0 6 228 6 234 196 0.0 6 192 4 235 222 0.0 6 216 5 1 236 160 0.0 6 160 237 226 0.0 6 224 2 238 226 0.0 6 221 5 239 338 0.0 6 332 6 240 368 0.0 6 358 9 0 0 1 241 792 0.0 6 778 12 2 242 562 0.0 6 550 12 243 649 0.0 6 634 15 244 411 0.0 6 397 14 245 804 0.0 6 793 11 246 180 0.0 6 175 4 1 247 293 0.0 6 290 3 248 157 0.0 6 150 7 249 645 0.0 6 638 7 250 344 0.0 6 338 6 251 376 0.0 6 370 5 1 252 325 0.0 6 318 7 253 207 0.0 6 203 4 254 676 0.0 6 668 8 255 332 0.0 6 327 4 0 0 1 256 249 0.0 6 242 7 257 311 0.0 6 304 7 258 406 0.0 6 397 8 0 0 0 0 1 259 541 0.0 6 526 15 260 796 0.0 6 778 18 261 271 0.0 6 266 4 1 262 300 0.0 6 294 6 263 727 0.0 6 718 9 264 266 0.0 6 262 3 0 0 1 265 466 0.0 6 460 6 266 130 0.0 6 125 5 267 404 0.0 6 391 13 268 346 0.0 6 333 12 0 1 269 721 0.0 6 714 6 0 1 270 168 0.0 6 161 7 271 289 0.0 6 278 11 272 1250 0.0 6 1224 26 273 455 0.0 6 443 12 274 1416 0.0 6 1389 26 1 275 329 0.0 6 318 11 276 1214 0.0 6 1179 32 3 277 5181 0.0 6 5126 53 1 0 0 1 278 1362 0.0 6 1319 36 2 1 2 0 2 279 20688 0.0 6 20081 587 18 1 1 280 209 0.0 6 182 23 4 281 27 0.0 6 22 2 3 282 3 0.0 6 3 283 2 0.0 6 2 284 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 328 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 8 0.0 6 8 72 4 0.0 6 4 73 2 0.0 6 2 74 2 0.0 6 2 75 7 0.0 6 7 76 3 0.0 6 3 77 5 0.0 6 5 78 8 0.0 6 7 1 79 11 0.0 6 11 80 7 0.0 6 7 81 10 0.0 6 10 82 8 0.0 6 8 83 5 0.0 6 5 84 5 0.0 6 5 85 1 0.0 6 1 86 3 0.0 6 3 87 4 0.0 6 4 88 3 0.0 6 3 89 6 0.0 6 6 90 1 0.0 6 1 91 4 0.0 6 4 92 5 0.0 6 5 93 3 0.0 6 3 94 3 0.0 6 3 95 6 0.0 6 6 96 4 0.0 6 4 98 2 0.0 6 2 99 4 0.0 6 4 100 2 0.0 6 2 101 2 0.0 6 2 102 5 0.0 6 5 103 3 0.0 6 3 104 1 0.0 6 1 105 3 0.0 6 3 106 2 0.0 6 2 107 1 0.0 6 1 108 2 0.0 6 2 109 7 0.0 6 7 110 5 0.0 6 5 111 2 0.0 6 2 112 1 0.0 6 1 113 4 0.0 6 4 114 3 0.0 6 3 115 3 0.0 6 3 117 1 0.0 6 1 118 2 0.0 6 2 119 1 0.0 6 1 120 1 0.0 6 1 121 1 0.0 6 1 122 4 0.0 6 4 123 1 0.0 6 1 125 4 0.0 6 4 126 2 0.0 6 2 127 4 0.0 6 4 129 1 0.0 6 1 131 1 0.0 6 1 132 2 0.0 6 2 133 1 0.0 6 1 134 5 0.0 6 5 135 1 0.0 6 1 136 1 0.0 6 1 137 2 0.0 6 2 138 2 0.0 6 1 1 139 1 0.0 6 1 140 5 0.0 6 5 141 2 0.0 6 2 142 2 0.0 6 2 143 1 0.0 6 1 144 3 0.0 6 3 145 1 0.0 6 1 146 3 0.0 6 3 147 1 0.0 6 1 148 2 0.0 6 2 149 3 0.0 6 3 151 3 0.0 6 3 152 1 0.0 6 1 154 2 0.0 6 2 155 1 0.0 6 1 156 2 0.0 6 2 157 2 0.0 6 2 158 4 0.0 6 4 160 1 0.0 6 1 161 1 0.0 6 1 162 3 0.0 6 3 164 3 0.0 6 3 165 2 0.0 6 2 166 1 0.0 6 1 167 2 0.0 6 2 169 1 0.0 6 1 170 2 0.0 6 2 172 2 0.0 6 2 173 1 0.0 6 1 174 1 0.0 6 1 175 2 0.0 6 2 176 1 0.0 6 1 180 1 0.0 6 0 0 0 0 0 0 1 181 2 0.0 6 2 182 2 0.0 6 2 185 2 0.0 6 2 187 2 0.0 6 2 188 2 0.0 6 2 189 1 0.0 6 1 191 1 0.0 6 1 193 3 0.0 6 3 194 1 0.0 6 1 196 1 0.0 6 1 197 1 0.0 6 1 200 2 0.0 6 2 201 1 0.0 6 1 202 1 0.0 6 1 203 1 0.0 6 1 204 1 0.0 6 1 205 1 0.0 6 1 206 2 0.0 6 2 207 1 0.0 6 1 208 1 0.0 6 1 212 1 0.0 6 1 213 1 0.0 6 1 214 4 0.0 6 4 215 1 0.0 6 1 216 1 0.0 6 1 218 1 0.0 6 1 220 1 0.0 6 1 226 1 0.0 6 1 227 2 0.0 6 2 229 2 0.0 6 2 241 2 0.0 6 2 244 1 0.0 6 1 247 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 72456 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1138.4 0 1 4 1 284.6 1 1 7 1 4.4 2 0 1 8 1 1.1 2 1 13 3 0.0 3 1 2 14 7 0.0 4 6 1 16 2 0.0 4 2 17 14 0.0 5 13 1 18 1 0.0 5 1 19 30 0.0 5 29 1 20 61 0.0 6 58 2 1 21 47 0.0 6 44 3 22 47 0.0 6 45 1 1 23 15 0.0 6 15 24 16 0.0 6 16 25 7 0.0 6 7 26 12 0.0 6 11 1 27 6 0.0 6 6 28 10 0.0 6 10 29 8 0.0 6 7 1 30 19 0.0 6 18 1 31 6 0.0 6 6 32 12 0.0 6 11 1 33 9 0.0 6 8 1 34 7 0.0 6 6 1 35 6 0.0 6 5 0 1 36 10 0.0 6 9 1 37 7 0.0 6 7 38 6 0.0 6 4 2 39 9 0.0 6 9 40 5 0.0 6 5 41 4 0.0 6 4 42 6 0.0 6 4 2 43 6 0.0 6 6 44 15 0.0 6 15 45 7 0.0 6 7 46 16 0.0 6 13 3 47 9 0.0 6 7 2 48 13 0.0 6 13 49 18 0.0 6 14 3 1 50 17 0.0 6 16 1 51 19 0.0 6 17 1 0 0 1 52 25 0.0 6 22 3 53 15 0.0 6 12 3 54 10 0.0 6 10 55 27 0.0 6 20 4 2 0 1 56 31 0.0 6 29 2 57 30 0.0 6 28 2 58 12 0.0 6 11 0 0 0 0 1 59 9 0.0 6 7 2 60 12 0.0 6 11 0 1 61 9 0.0 6 6 2 1 62 14 0.0 6 13 1 63 7 0.0 6 7 64 26 0.0 6 21 4 1 65 14 0.0 6 12 2 66 16 0.0 6 15 0 0 0 1 67 15 0.0 6 14 1 68 27 0.0 6 25 2 69 42 0.0 6 40 2 70 137 0.0 6 130 6 0 0 0 0 1 71 35 0.0 6 31 3 0 1 72 70 0.0 6 67 3 73 27 0.0 6 25 1 0 0 1 74 34 0.0 6 29 4 0 1 75 18 0.0 6 16 2 76 14 0.0 6 12 1 0 0 1 77 25 0.0 6 24 1 78 35 0.0 6 34 1 79 10 0.0 6 10 80 32 0.0 6 31 0 1 81 28 0.0 6 27 1 82 28 0.0 6 25 2 0 0 1 83 28 0.0 6 25 3 84 46 0.0 6 44 2 85 29 0.0 6 29 86 32 0.0 6 28 4 87 40 0.0 6 37 3 88 21 0.0 6 20 1 89 17 0.0 6 17 90 39 0.0 6 37 2 91 25 0.0 6 23 1 1 92 22 0.0 6 21 1 93 31 0.0 6 30 1 94 70 0.0 6 67 3 95 24 0.0 6 23 1 96 59 0.0 6 54 4 1 97 37 0.0 6 35 1 0 0 1 98 32 0.0 6 30 2 99 70 0.0 6 63 5 2 100 47 0.0 6 45 2 101 48 0.0 6 43 5 102 85 0.0 6 83 2 103 86 0.0 6 83 2 1 104 76 0.0 6 75 1 105 63 0.0 6 59 4 106 55 0.0 6 50 5 107 82 0.0 6 81 0 1 108 48 0.0 6 46 1 0 1 109 53 0.0 6 49 4 110 50 0.0 6 46 4 111 56 0.0 6 54 2 112 71 0.0 6 69 2 113 58 0.0 6 55 2 1 114 72 0.0 6 64 5 3 115 88 0.0 6 86 2 116 79 0.0 6 76 2 1 117 66 0.0 6 64 2 118 83 0.0 6 81 2 119 104 0.0 6 99 5 120 111 0.0 6 103 8 121 91 0.0 6 86 4 0 0 1 122 85 0.0 6 82 3 123 88 0.0 6 83 4 1 124 123 0.0 6 118 4 0 0 1 125 85 0.0 6 81 3 0 1 126 67 0.0 6 64 3 127 118 0.0 6 114 4 128 134 0.0 6 132 2 129 96 0.0 6 96 130 118 0.0 6 115 2 1 131 149 0.0 6 145 4 132 148 0.0 6 141 7 133 105 0.0 6 102 3 134 156 0.0 6 149 6 0 0 1 135 285 0.0 6 272 11 0 1 1 136 195 0.0 6 192 3 137 222 0.0 6 215 7 138 197 0.0 6 192 5 139 216 0.0 6 210 5 0 0 1 140 315 0.0 6 310 4 1 141 364 0.0 6 353 11 142 498 0.0 6 489 7 2 143 401 0.0 6 392 7 1 1 144 582 0.0 6 563 19 145 520 0.0 6 513 5 2 146 365 0.0 6 359 6 147 398 0.0 6 390 6 0 1 0 0 1 148 441 0.0 6 436 5 149 569 0.0 6 560 6 1 0 1 0 1 150 694 0.0 6 681 11 1 0 1 151 522 0.0 6 506 15 0 1 152 670 0.0 6 656 13 0 0 0 1 153 716 0.0 6 697 17 2 154 633 0.0 6 617 15 0 1 155 689 0.0 6 674 12 2 1 156 566 0.0 6 558 8 157 524 0.0 6 514 10 158 675 0.0 6 663 12 159 378 0.0 6 365 13 160 624 0.0 6 609 14 0 1 161 499 0.0 6 487 9 0 1 1 1 162 371 0.0 6 360 11 163 606 0.0 6 597 8 1 164 505 0.0 6 496 9 165 660 0.0 6 638 20 1 1 166 653 0.0 6 639 12 2 167 447 0.0 6 438 9 168 595 0.0 6 579 16 169 365 0.0 6 358 7 170 393 0.0 6 389 4 171 619 0.0 6 605 11 0 1 1 0 1 172 339 0.0 6 332 7 173 466 0.0 6 455 10 0 0 1 174 638 0.0 6 607 30 1 175 1024 0.0 6 992 30 1 1 176 719 0.0 6 704 15 177 658 0.0 6 640 17 0 1 178 585 0.0 6 575 10 179 654 0.0 6 634 20 180 612 0.0 6 599 12 0 0 1 181 837 0.0 6 813 23 1 182 470 0.0 6 447 21 2 183 820 0.0 6 801 17 1 1 184 185 0.0 6 178 6 0 0 1 185 221 0.0 6 211 10 186 890 0.0 6 865 24 0 0 0 1 187 1163 0.0 6 1134 27 0 0 1 0 1 188 1493 0.0 6 1445 45 2 0 1 189 1027 0.0 6 993 33 0 0 1 190 1816 0.0 6 1761 54 1 191 1447 0.0 6 1408 35 4 192 1271 0.0 6 1231 32 4 0 4 193 669 0.0 6 651 18 194 1272 0.0 6 1228 41 3 195 1142 0.0 6 1108 32 1 0 1 196 3214 0.0 6 3132 77 1 1 3 197 746 0.0 6 712 31 3 198 1476 0.0 6 1429 47 199 3325 0.0 6 3245 76 0 0 4 200 931 0.0 6 896 34 0 0 1 201 1547 0.0 6 1511 35 1 202 819 0.0 6 807 12 203 564 0.0 6 546 18 204 797 0.0 6 782 15 205 312 0.0 6 304 8 206 394 0.0 6 388 5 1 207 198 0.0 6 193 5 208 401 0.0 6 387 13 0 0 1 209 397 0.0 6 390 7 210 378 0.0 6 367 11 211 609 0.0 6 588 21 212 696 0.0 6 680 15 0 0 0 0 1 213 118 0.0 6 115 2 1 214 293 0.0 6 283 10 215 352 0.0 6 338 14 216 486 0.0 6 471 15 217 1113 0.0 6 1081 31 1 218 520 0.0 6 507 13 219 680 0.0 6 662 17 0 0 1 220 381 0.0 6 369 11 0 0 1 221 783 0.0 6 765 17 0 0 1 222 314 0.0 6 302 12 223 496 0.0 6 488 7 0 0 1 224 410 0.0 6 393 14 0 1 2 225 768 0.0 6 750 18 226 587 0.0 6 574 11 1 0 1 227 459 0.0 6 447 12 228 245 0.0 6 239 6 229 555 0.0 6 536 19 230 265 0.0 6 262 2 1 231 348 0.0 6 341 7 232 347 0.0 6 341 6 233 462 0.0 6 452 10 234 188 0.0 6 182 5 1 235 195 0.0 6 192 3 236 52 0.0 6 50 2 237 88 0.0 6 84 3 0 0 1 238 35 0.0 6 34 1 239 28 0.0 6 25 3 240 95 0.0 6 91 3 0 1 241 175 0.0 6 171 4 242 276 0.0 6 271 5 243 260 0.0 6 258 2 244 356 0.0 6 351 5 245 101 0.0 6 99 1 0 0 1 246 101 0.0 6 101 247 22 0.0 6 21 1 248 22 0.0 6 22 249 59 0.0 6 59 250 32 0.0 6 32 251 57 0.0 6 56 1 252 9 0.0 6 9 253 16 0.0 6 15 1 254 44 0.0 6 43 1 255 4 0.0 6 4 256 1 0.0 6 1 257 5 0.0 6 5 258 4 0.0 6 4 259 2 0.0 6 2 262 1 0.0 6 1 264 1 0.0 6 1 279 1 0.0 6 1 281 8 0.0 6 8 282 26 0.0 6 23 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 369 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 1 0.0 6 0 1 60 3 0.0 6 3 67 1 0.0 6 1 69 14 0.0 6 14 70 13 0.0 6 13 71 5 0.0 6 5 72 6 0.0 6 6 73 1 0.0 6 1 74 6 0.0 6 6 75 1 0.0 6 1 76 3 0.0 6 3 77 4 0.0 6 4 78 10 0.0 6 10 79 5 0.0 6 5 80 6 0.0 6 6 81 6 0.0 6 6 82 1 0.0 6 1 83 4 0.0 6 4 84 3 0.0 6 3 85 6 0.0 6 6 86 7 0.0 6 7 87 5 0.0 6 5 88 3 0.0 6 3 89 11 0.0 6 11 90 7 0.0 6 7 91 2 0.0 6 2 92 6 0.0 6 6 93 1 0.0 6 1 94 3 0.0 6 3 95 1 0.0 6 1 96 2 0.0 6 2 98 5 0.0 6 5 99 6 0.0 6 6 100 2 0.0 6 2 101 2 0.0 6 2 102 4 0.0 6 4 103 6 0.0 6 6 104 5 0.0 6 5 105 2 0.0 6 2 106 8 0.0 6 8 107 8 0.0 6 8 108 1 0.0 6 1 109 3 0.0 6 3 110 8 0.0 6 8 111 8 0.0 6 8 114 7 0.0 6 7 115 6 0.0 6 6 116 4 0.0 6 4 117 1 0.0 6 1 118 1 0.0 6 1 119 3 0.0 6 3 121 5 0.0 6 5 122 2 0.0 6 2 123 1 0.0 6 0 1 124 13 0.0 6 13 125 1 0.0 6 1 127 2 0.0 6 2 128 2 0.0 6 2 129 3 0.0 6 3 130 2 0.0 6 2 131 2 0.0 6 2 132 2 0.0 6 2 133 1 0.0 6 1 134 1 0.0 6 1 135 1 0.0 6 1 136 4 0.0 6 4 137 3 0.0 6 3 138 2 0.0 6 2 139 3 0.0 6 3 140 1 0.0 6 1 141 1 0.0 6 1 142 4 0.0 6 4 143 2 0.0 6 2 144 3 0.0 6 3 145 4 0.0 6 4 146 2 0.0 6 2 147 1 0.0 6 1 148 1 0.0 6 1 149 3 0.0 6 3 150 1 0.0 6 1 151 2 0.0 6 2 152 2 0.0 6 1 0 0 0 1 153 2 0.0 6 2 154 2 0.0 6 2 155 4 0.0 6 4 156 1 0.0 6 1 157 1 0.0 6 1 158 1 0.0 6 1 159 1 0.0 6 1 162 1 0.0 6 1 164 5 0.0 6 5 167 3 0.0 6 3 168 1 0.0 6 1 169 2 0.0 6 2 170 2 0.0 6 2 171 2 0.0 6 2 172 1 0.0 6 1 173 1 0.0 6 1 175 1 0.0 6 1 176 1 0.0 6 1 177 3 0.0 6 3 178 1 0.0 6 1 179 2 0.0 6 2 180 2 0.0 6 2 181 1 0.0 6 1 183 2 0.0 6 2 184 1 0.0 6 1 191 2 0.0 6 2 195 1 0.0 6 1 200 3 0.0 6 3 201 1 0.0 6 1 203 1 0.0 6 1 204 1 0.0 6 1 208 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_10_195_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_10_422_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_10_195_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_10_422_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.96 s (82 µs/read; 0.73 M reads/minute). === Summary === Total read pairs processed: 48,384 Read 1 with adapter: 48,375 (100.0%) Read 2 with adapter: 48,361 (100.0%) == Read fate breakdown == Pairs that were too short: 5 (0.0%) Pairs discarded as untrimmed: 32 (0.1%) Pairs written (passing filters): 48,347 (99.9%) Total basepairs processed: 22,090,931 bp Read 1: 12,300,619 bp Read 2: 9,790,312 bp Total written (filtered): 3,137,014 bp (14.2%) Read 1: 1,572,901 bp Read 2: 1,564,113 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 47929 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 756.0 0 1 1 4 1 189.0 1 1 5 1 47.2 1 1 7 1 3.0 2 0 0 1 9 3 0.2 2 3 10 1 0.0 3 1 12 1 0.0 3 1 15 1 0.0 4 1 16 1 0.0 4 1 17 3 0.0 5 3 20 30 0.0 6 29 1 21 15 0.0 6 15 22 15 0.0 6 15 23 16 0.0 6 15 1 24 7 0.0 6 7 25 4 0.0 6 4 26 12 0.0 6 12 27 5 0.0 6 5 28 4 0.0 6 4 29 1 0.0 6 1 30 4 0.0 6 4 31 3 0.0 6 3 32 3 0.0 6 3 33 3 0.0 6 3 34 2 0.0 6 2 35 4 0.0 6 4 36 1 0.0 6 1 37 2 0.0 6 2 38 3 0.0 6 3 39 3 0.0 6 3 41 4 0.0 6 3 1 42 2 0.0 6 2 43 2 0.0 6 1 1 44 2 0.0 6 1 0 0 0 0 0 1 45 2 0.0 6 2 46 3 0.0 6 3 47 4 0.0 6 4 48 1 0.0 6 1 49 2 0.0 6 2 50 2 0.0 6 2 51 2 0.0 6 2 52 2 0.0 6 2 53 2 0.0 6 2 54 5 0.0 6 5 55 3 0.0 6 3 56 4 0.0 6 4 57 4 0.0 6 4 58 4 0.0 6 2 2 59 1 0.0 6 1 60 3 0.0 6 3 61 2 0.0 6 2 62 7 0.0 6 7 63 7 0.0 6 6 1 64 3 0.0 6 3 65 2 0.0 6 2 66 1 0.0 6 1 67 5 0.0 6 4 1 68 4 0.0 6 4 69 3 0.0 6 3 70 2 0.0 6 2 71 5 0.0 6 5 72 8 0.0 6 8 73 3 0.0 6 3 74 6 0.0 6 6 75 5 0.0 6 5 76 4 0.0 6 4 77 4 0.0 6 4 78 3 0.0 6 3 79 5 0.0 6 5 80 6 0.0 6 6 81 4 0.0 6 4 82 5 0.0 6 4 1 83 9 0.0 6 9 84 9 0.0 6 9 85 6 0.0 6 6 86 8 0.0 6 7 1 87 10 0.0 6 10 88 2 0.0 6 1 1 89 5 0.0 6 5 90 5 0.0 6 5 91 3 0.0 6 3 92 7 0.0 6 6 1 93 6 0.0 6 6 94 4 0.0 6 4 95 7 0.0 6 7 96 4 0.0 6 4 97 5 0.0 6 5 98 7 0.0 6 6 1 99 6 0.0 6 6 100 6 0.0 6 6 101 14 0.0 6 14 102 7 0.0 6 7 103 9 0.0 6 7 2 104 12 0.0 6 12 105 15 0.0 6 15 106 5 0.0 6 5 107 5 0.0 6 4 1 108 14 0.0 6 13 1 109 9 0.0 6 9 110 16 0.0 6 16 111 15 0.0 6 15 112 29 0.0 6 29 113 20 0.0 6 18 2 114 17 0.0 6 17 115 13 0.0 6 12 1 116 24 0.0 6 23 1 117 16 0.0 6 15 0 1 118 11 0.0 6 11 119 24 0.0 6 24 120 13 0.0 6 13 121 20 0.0 6 20 122 21 0.0 6 21 123 18 0.0 6 17 1 124 19 0.0 6 19 125 24 0.0 6 24 126 21 0.0 6 21 127 14 0.0 6 14 128 23 0.0 6 22 1 129 20 0.0 6 20 130 30 0.0 6 30 131 31 0.0 6 30 1 132 26 0.0 6 26 133 17 0.0 6 17 134 35 0.0 6 35 135 27 0.0 6 27 136 36 0.0 6 36 137 31 0.0 6 30 1 138 44 0.0 6 44 139 35 0.0 6 34 1 140 46 0.0 6 46 141 47 0.0 6 45 1 0 1 142 66 0.0 6 66 143 203 0.0 6 203 144 354 0.0 6 354 145 395 0.0 6 395 146 326 0.0 6 324 2 147 373 0.0 6 372 1 148 492 0.0 6 491 0 1 149 594 0.0 6 592 1 0 1 150 479 0.0 6 477 2 151 515 0.0 6 511 4 152 555 0.0 6 555 153 541 0.0 6 539 2 154 343 0.0 6 343 155 343 0.0 6 343 156 329 0.0 6 328 1 157 316 0.0 6 316 158 199 0.0 6 199 159 291 0.0 6 291 160 265 0.0 6 265 161 296 0.0 6 296 162 256 0.0 6 255 1 163 312 0.0 6 309 3 164 282 0.0 6 281 1 165 296 0.0 6 295 1 166 229 0.0 6 227 2 167 204 0.0 6 203 1 168 175 0.0 6 175 169 173 0.0 6 172 1 170 171 0.0 6 171 171 150 0.0 6 149 1 172 224 0.0 6 224 173 255 0.0 6 255 174 208 0.0 6 206 2 175 181 0.0 6 179 2 176 145 0.0 6 142 3 177 143 0.0 6 141 1 1 178 155 0.0 6 154 1 179 149 0.0 6 148 1 180 127 0.0 6 127 181 314 0.0 6 314 182 167 0.0 6 166 1 183 157 0.0 6 157 184 200 0.0 6 198 1 1 185 153 0.0 6 149 4 186 162 0.0 6 160 1 0 0 1 187 164 0.0 6 158 6 188 178 0.0 6 178 189 176 0.0 6 173 3 190 179 0.0 6 176 3 191 167 0.0 6 166 1 192 185 0.0 6 182 3 193 239 0.0 6 236 2 1 194 241 0.0 6 237 4 195 288 0.0 6 284 4 196 306 0.0 6 302 4 197 324 0.0 6 324 198 337 0.0 6 333 4 199 264 0.0 6 260 3 1 200 212 0.0 6 207 5 201 259 0.0 6 255 4 202 262 0.0 6 257 5 203 295 0.0 6 292 3 204 264 0.0 6 261 3 205 306 0.0 6 303 3 206 336 0.0 6 334 2 207 308 0.0 6 306 2 208 335 0.0 6 329 5 1 209 268 0.0 6 266 2 210 254 0.0 6 253 1 211 225 0.0 6 221 3 0 0 0 1 212 243 0.0 6 242 1 213 255 0.0 6 252 3 214 274 0.0 6 269 4 1 215 300 0.0 6 298 2 216 225 0.0 6 224 1 217 301 0.0 6 299 2 218 265 0.0 6 265 219 200 0.0 6 199 1 220 206 0.0 6 201 5 221 183 0.0 6 183 222 208 0.0 6 205 2 1 223 175 0.0 6 174 1 224 156 0.0 6 155 1 225 250 0.0 6 246 4 226 391 0.0 6 387 3 0 1 227 253 0.0 6 249 4 228 233 0.0 6 231 1 1 229 194 0.0 6 190 4 230 214 0.0 6 209 5 231 195 0.0 6 193 2 232 185 0.0 6 182 3 233 163 0.0 6 161 1 1 234 142 0.0 6 140 2 235 148 0.0 6 146 2 236 138 0.0 6 135 3 237 160 0.0 6 157 2 1 238 157 0.0 6 154 2 0 0 0 0 1 239 216 0.0 6 212 3 1 240 271 0.0 6 268 3 241 403 0.0 6 398 3 2 242 328 0.0 6 325 2 1 243 285 0.0 6 281 3 1 244 249 0.0 6 243 6 245 335 0.0 6 331 3 0 1 246 105 0.0 6 103 2 247 140 0.0 6 138 2 248 140 0.0 6 139 1 249 264 0.0 6 259 5 250 192 0.0 6 191 1 251 199 0.0 6 197 2 252 147 0.0 6 145 2 253 174 0.0 6 168 6 254 295 0.0 6 291 4 255 156 0.0 6 156 256 153 0.0 6 150 3 257 162 0.0 6 158 4 258 232 0.0 6 228 4 259 301 0.0 6 299 2 260 342 0.0 6 336 4 1 1 261 147 0.0 6 140 7 262 165 0.0 6 161 4 263 309 0.0 6 304 4 1 264 101 0.0 6 99 2 265 194 0.0 6 194 266 61 0.0 6 58 3 267 183 0.0 6 178 5 268 164 0.0 6 163 1 269 251 0.0 6 247 4 270 117 0.0 6 114 3 271 165 0.0 6 160 5 272 412 0.0 6 407 5 273 225 0.0 6 223 2 274 447 0.0 6 441 6 275 253 0.0 6 246 7 276 684 0.0 6 670 14 277 1494 0.0 6 1471 23 278 496 0.0 6 480 15 0 1 279 11144 0.0 6 10853 281 9 1 280 91 0.0 6 78 11 2 281 4 0.0 6 3 1 282 1 0.0 6 1 283 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 446 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 1 0.0 6 0 1 63 51 0.0 6 50 1 67 3 0.0 6 3 72 2 0.0 6 2 73 8 0.0 6 8 74 9 0.0 6 9 75 6 0.0 6 6 76 8 0.0 6 8 77 14 0.0 6 14 78 7 0.0 6 7 79 5 0.0 6 5 80 11 0.0 6 11 81 6 0.0 6 6 82 16 0.0 6 16 83 8 0.0 6 8 84 7 0.0 6 7 85 5 0.0 6 5 86 7 0.0 6 6 1 87 8 0.0 6 8 88 9 0.0 6 9 89 7 0.0 6 7 90 3 0.0 6 3 91 4 0.0 6 4 92 11 0.0 6 11 93 3 0.0 6 3 94 4 0.0 6 4 95 5 0.0 6 5 96 3 0.0 6 3 97 1 0.0 6 1 98 4 0.0 6 4 99 2 0.0 6 2 100 1 0.0 6 1 101 5 0.0 6 5 102 7 0.0 6 7 103 4 0.0 6 4 104 3 0.0 6 3 105 2 0.0 6 2 106 2 0.0 6 2 107 3 0.0 6 3 108 4 0.0 6 4 109 7 0.0 6 7 110 7 0.0 6 7 111 2 0.0 6 2 112 3 0.0 6 3 113 5 0.0 6 5 114 3 0.0 6 3 115 1 0.0 6 1 116 3 0.0 6 3 117 3 0.0 6 3 121 1 0.0 6 1 122 2 0.0 6 2 123 1 0.0 6 1 125 1 0.0 6 1 127 3 0.0 6 3 128 2 0.0 6 2 129 3 0.0 6 3 130 2 0.0 6 2 131 3 0.0 6 3 132 2 0.0 6 2 133 3 0.0 6 3 134 2 0.0 6 2 138 3 0.0 6 2 0 0 0 0 1 139 1 0.0 6 1 140 1 0.0 6 1 141 2 0.0 6 2 142 2 0.0 6 2 143 1 0.0 6 1 145 6 0.0 6 6 146 2 0.0 6 2 147 1 0.0 6 1 148 2 0.0 6 2 149 2 0.0 6 2 151 1 0.0 6 1 152 1 0.0 6 1 153 1 0.0 6 1 154 2 0.0 6 2 155 1 0.0 6 1 158 2 0.0 6 2 159 2 0.0 6 2 160 1 0.0 6 1 161 1 0.0 6 1 162 3 0.0 6 3 163 3 0.0 6 3 164 1 0.0 6 1 165 2 0.0 6 2 166 3 0.0 6 3 167 1 0.0 6 1 168 1 0.0 6 1 172 2 0.0 6 2 173 3 0.0 6 3 174 1 0.0 6 1 176 1 0.0 6 1 177 1 0.0 6 1 180 3 0.0 6 3 181 1 0.0 6 1 183 2 0.0 6 2 184 2 0.0 6 2 185 3 0.0 6 3 186 3 0.0 6 3 188 2 0.0 6 2 189 1 0.0 6 1 191 1 0.0 6 1 193 2 0.0 6 2 194 1 0.0 6 1 195 1 0.0 6 1 196 3 0.0 6 3 197 2 0.0 6 2 198 2 0.0 6 2 199 1 0.0 6 1 202 1 0.0 6 1 205 2 0.0 6 2 206 2 0.0 6 2 207 3 0.0 6 3 208 1 0.0 6 1 209 1 0.0 6 1 210 1 0.0 6 1 212 2 0.0 6 2 218 1 0.0 6 1 219 1 0.0 6 1 220 2 0.0 6 2 224 2 0.0 6 2 225 1 0.0 6 1 228 1 0.0 6 1 229 1 0.0 6 1 233 1 0.0 6 1 238 1 0.0 6 1 243 1 0.0 6 1 254 1 0.0 6 1 259 2 0.0 6 2 290 2 0.0 6 2 293 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 47948 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 3.0 2 1 9 1 0.2 2 1 10 1 0.0 3 1 12 2 0.0 3 2 13 3 0.0 3 2 1 14 3 0.0 4 3 15 1 0.0 4 1 16 8 0.0 4 6 2 17 12 0.0 5 9 3 18 13 0.0 5 12 1 19 5 0.0 5 5 20 35 0.0 6 33 2 21 23 0.0 6 23 22 12 0.0 6 12 23 11 0.0 6 11 24 17 0.0 6 14 1 1 0 1 25 10 0.0 6 10 26 9 0.0 6 8 0 1 27 7 0.0 6 7 28 8 0.0 6 8 29 15 0.0 6 13 2 30 8 0.0 6 8 31 11 0.0 6 11 32 10 0.0 6 9 1 33 3 0.0 6 2 1 34 8 0.0 6 7 0 1 35 6 0.0 6 6 36 8 0.0 6 6 2 37 7 0.0 6 7 38 4 0.0 6 4 39 2 0.0 6 2 40 4 0.0 6 3 1 41 12 0.0 6 12 42 6 0.0 6 6 43 4 0.0 6 3 1 44 9 0.0 6 7 2 45 7 0.0 6 7 46 14 0.0 6 13 1 47 8 0.0 6 7 1 48 6 0.0 6 6 49 6 0.0 6 5 1 50 5 0.0 6 4 1 51 12 0.0 6 10 2 52 20 0.0 6 17 3 53 12 0.0 6 10 2 54 21 0.0 6 20 1 55 14 0.0 6 13 0 0 0 1 56 13 0.0 6 12 1 57 18 0.0 6 17 0 1 58 12 0.0 6 11 1 59 9 0.0 6 9 60 13 0.0 6 10 3 61 7 0.0 6 7 62 16 0.0 6 16 63 12 0.0 6 11 1 64 11 0.0 6 11 65 18 0.0 6 14 2 2 66 13 0.0 6 13 67 85 0.0 6 82 1 0 0 1 1 68 35 0.0 6 31 3 0 1 69 46 0.0 6 44 2 70 10 0.0 6 10 71 23 0.0 6 22 1 72 18 0.0 6 17 1 73 13 0.0 6 13 74 12 0.0 6 12 75 20 0.0 6 18 2 76 19 0.0 6 19 77 19 0.0 6 17 1 0 0 1 78 15 0.0 6 14 1 79 19 0.0 6 18 1 80 33 0.0 6 31 1 1 81 18 0.0 6 18 82 37 0.0 6 37 83 45 0.0 6 44 1 84 30 0.0 6 27 2 1 85 22 0.0 6 21 1 86 12 0.0 6 11 0 1 87 31 0.0 6 29 2 88 17 0.0 6 17 89 30 0.0 6 30 90 31 0.0 6 27 4 91 39 0.0 6 36 3 92 29 0.0 6 28 1 93 51 0.0 6 48 2 1 94 54 0.0 6 51 3 95 28 0.0 6 27 0 1 96 34 0.0 6 28 4 1 0 1 97 32 0.0 6 30 2 98 35 0.0 6 34 1 99 40 0.0 6 38 2 100 42 0.0 6 39 2 0 0 1 101 50 0.0 6 49 0 1 102 52 0.0 6 48 4 103 72 0.0 6 70 2 104 64 0.0 6 59 5 105 45 0.0 6 45 106 45 0.0 6 41 3 1 107 66 0.0 6 61 4 0 1 108 57 0.0 6 54 3 109 70 0.0 6 69 0 0 0 0 0 1 110 56 0.0 6 55 1 111 59 0.0 6 58 1 112 57 0.0 6 56 1 113 71 0.0 6 68 3 114 72 0.0 6 70 1 0 0 1 115 74 0.0 6 72 1 0 0 1 116 61 0.0 6 60 1 117 64 0.0 6 62 2 118 88 0.0 6 87 0 0 0 0 0 1 119 92 0.0 6 89 3 120 82 0.0 6 81 1 121 58 0.0 6 54 3 1 122 98 0.0 6 94 4 123 79 0.0 6 79 124 131 0.0 6 131 125 122 0.0 6 119 3 126 113 0.0 6 110 1 1 0 0 0 1 127 167 0.0 6 165 2 128 133 0.0 6 130 2 0 0 0 0 1 129 143 0.0 6 139 4 130 137 0.0 6 133 3 1 131 217 0.0 6 217 132 202 0.0 6 194 6 1 0 0 1 133 208 0.0 6 206 2 134 231 0.0 6 229 2 135 272 0.0 6 269 2 1 136 219 0.0 6 217 2 137 221 0.0 6 216 4 0 0 1 138 199 0.0 6 197 2 139 262 0.0 6 261 1 140 388 0.0 6 382 6 141 555 0.0 6 554 1 142 668 0.0 6 658 7 2 0 0 0 1 143 532 0.0 6 526 5 0 0 1 144 667 0.0 6 659 8 145 654 0.0 6 646 8 146 685 0.0 6 680 5 147 627 0.0 6 624 3 148 760 0.0 6 755 5 149 766 0.0 6 761 4 0 0 1 150 1113 0.0 6 1102 11 151 582 0.0 6 574 7 0 0 0 0 1 152 831 0.0 6 822 8 0 0 1 153 631 0.0 6 625 6 154 622 0.0 6 615 7 155 500 0.0 6 492 8 156 523 0.0 6 514 9 157 586 0.0 6 581 5 158 575 0.0 6 568 7 159 425 0.0 6 420 4 1 160 490 0.0 6 487 3 161 405 0.0 6 402 2 0 1 162 334 0.0 6 326 8 163 400 0.0 6 389 9 2 164 408 0.0 6 400 7 1 165 415 0.0 6 407 7 0 0 1 166 451 0.0 6 445 6 167 328 0.0 6 319 8 0 0 0 1 168 494 0.0 6 487 6 0 1 169 299 0.0 6 293 6 170 310 0.0 6 306 4 171 437 0.0 6 431 5 0 1 172 347 0.0 6 344 3 173 445 0.0 6 435 9 0 0 1 174 535 0.0 6 527 8 175 708 0.0 6 696 12 176 547 0.0 6 536 10 1 177 476 0.0 6 467 7 1 0 1 178 422 0.0 6 413 8 0 1 179 361 0.0 6 355 5 1 180 276 0.0 6 267 8 0 0 0 0 1 181 369 0.0 6 361 8 182 164 0.0 6 156 8 183 467 0.0 6 458 6 1 0 1 1 184 316 0.0 6 310 6 185 442 0.0 6 430 12 186 691 0.0 6 671 17 1 0 2 187 615 0.0 6 599 16 188 616 0.0 6 600 15 1 189 487 0.0 6 474 12 1 190 768 0.0 6 754 13 0 1 191 820 0.0 6 798 21 0 0 1 192 484 0.0 6 473 9 0 2 193 264 0.0 6 253 11 194 549 0.0 6 537 11 1 195 737 0.0 6 719 18 196 1383 0.0 6 1354 26 0 1 2 197 412 0.0 6 393 19 198 799 0.0 6 780 18 0 0 1 199 1280 0.0 6 1253 27 200 574 0.0 6 552 21 0 0 1 201 713 0.0 6 689 24 202 392 0.0 6 381 11 203 358 0.0 6 351 7 204 463 0.0 6 457 5 0 1 205 137 0.0 6 132 5 206 152 0.0 6 149 3 207 98 0.0 6 94 4 208 178 0.0 6 172 4 1 0 1 209 138 0.0 6 132 6 210 148 0.0 6 144 4 211 126 0.0 6 121 5 212 218 0.0 6 214 4 213 117 0.0 6 114 3 214 362 0.0 6 356 6 215 341 0.0 6 336 5 216 201 0.0 6 194 6 0 1 217 287 0.0 6 274 11 1 0 1 218 190 0.0 6 183 7 219 246 0.0 6 242 4 220 71 0.0 6 68 3 221 246 0.0 6 242 4 222 271 0.0 6 268 3 223 292 0.0 6 281 11 224 181 0.0 6 172 9 225 212 0.0 6 200 10 0 1 1 226 124 0.0 6 122 2 227 132 0.0 6 130 2 228 72 0.0 6 72 229 225 0.0 6 217 7 0 0 1 230 95 0.0 6 92 2 0 1 231 158 0.0 6 152 6 232 190 0.0 6 186 4 233 103 0.0 6 99 4 234 80 0.0 6 75 5 235 34 0.0 6 33 1 236 6 0.0 6 6 237 10 0.0 6 9 0 0 0 1 238 8 0.0 6 7 1 239 21 0.0 6 20 1 240 46 0.0 6 45 1 241 106 0.0 6 103 3 242 154 0.0 6 152 2 243 93 0.0 6 91 2 244 53 0.0 6 53 245 32 0.0 6 31 1 246 39 0.0 6 39 247 7 0.0 6 6 1 248 23 0.0 6 22 1 249 10 0.0 6 9 1 250 17 0.0 6 17 251 14 0.0 6 14 252 5 0.0 6 5 253 2 0.0 6 2 259 1 0.0 6 1 277 1 0.0 6 1 278 13 0.0 6 13 279 22 0.0 6 22 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 413 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 1 0.0 6 1 67 1 0.0 6 1 70 1 0.0 6 1 71 4 0.0 6 4 72 3 0.0 6 3 73 10 0.0 6 10 74 22 0.0 6 22 75 5 0.0 6 5 76 11 0.0 6 11 77 11 0.0 6 11 78 11 0.0 6 11 79 13 0.0 6 13 80 14 0.0 6 13 1 81 19 0.0 6 18 1 82 9 0.0 6 9 83 10 0.0 6 10 84 6 0.0 6 6 85 11 0.0 6 11 86 3 0.0 6 3 87 5 0.0 6 5 88 15 0.0 6 15 89 2 0.0 6 2 90 11 0.0 6 11 91 5 0.0 6 5 92 6 0.0 6 6 93 4 0.0 6 4 94 4 0.0 6 4 95 1 0.0 6 1 96 3 0.0 6 3 97 6 0.0 6 6 98 5 0.0 6 5 99 4 0.0 6 4 100 4 0.0 6 4 101 4 0.0 6 4 102 6 0.0 6 6 103 6 0.0 6 6 104 2 0.0 6 2 105 2 0.0 6 2 106 1 0.0 6 1 108 2 0.0 6 2 109 5 0.0 6 5 110 6 0.0 6 6 111 6 0.0 6 6 112 2 0.0 6 2 113 5 0.0 6 5 114 1 0.0 6 1 115 3 0.0 6 3 116 2 0.0 6 1 0 1 117 4 0.0 6 3 0 0 0 1 118 3 0.0 6 3 121 1 0.0 6 1 122 3 0.0 6 3 123 2 0.0 6 2 124 2 0.0 6 2 125 1 0.0 6 1 127 1 0.0 6 1 129 2 0.0 6 2 130 2 0.0 6 2 131 3 0.0 6 3 132 2 0.0 6 2 133 4 0.0 6 4 134 4 0.0 6 4 135 2 0.0 6 2 137 3 0.0 6 3 138 1 0.0 6 1 139 2 0.0 6 2 141 3 0.0 6 3 142 1 0.0 6 1 143 2 0.0 6 2 144 2 0.0 6 2 145 2 0.0 6 2 146 2 0.0 6 2 147 1 0.0 6 1 148 1 0.0 6 1 149 1 0.0 6 1 150 9 0.0 6 9 152 1 0.0 6 1 153 1 0.0 6 1 155 1 0.0 6 1 157 4 0.0 6 4 158 1 0.0 6 1 159 1 0.0 6 1 160 2 0.0 6 2 161 2 0.0 6 2 162 1 0.0 6 1 163 2 0.0 6 2 164 3 0.0 6 3 165 1 0.0 6 1 167 3 0.0 6 3 168 1 0.0 6 1 172 1 0.0 6 1 173 3 0.0 6 3 175 1 0.0 6 1 176 1 0.0 6 1 179 2 0.0 6 2 180 1 0.0 6 1 182 3 0.0 6 3 183 1 0.0 6 1 184 2 0.0 6 2 185 2 0.0 6 2 186 1 0.0 6 1 188 3 0.0 6 3 197 2 0.0 6 2 199 1 0.0 6 1 203 2 0.0 6 2 205 1 0.0 6 1 207 1 0.0 6 1 219 1 0.0 6 1 238 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_11_196_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_11_423_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_11_196_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_11_423_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.56 s (88 µs/read; 0.68 M reads/minute). === Summary === Total read pairs processed: 51,861 Read 1 with adapter: 51,853 (100.0%) Read 2 with adapter: 51,838 (100.0%) == Read fate breakdown == Pairs that were too short: 16 (0.0%) Pairs discarded as untrimmed: 31 (0.1%) Pairs written (passing filters): 51,814 (99.9%) Total basepairs processed: 22,088,144 bp Read 1: 12,191,642 bp Read 2: 9,896,502 bp Total written (filtered): 3,244,443 bp (14.7%) Read 1: 1,709,390 bp Read 2: 1,535,053 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 50777 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 810.3 0 1 1 4 3 202.6 1 1 2 7 4 3.2 2 2 0 2 9 2 0.2 2 1 1 10 1 0.0 3 1 14 2 0.0 4 2 16 2 0.0 4 2 17 3 0.0 5 3 18 1 0.0 5 0 1 19 1 0.0 5 1 20 71 0.0 6 70 1 21 24 0.0 6 24 22 25 0.0 6 23 1 1 23 29 0.0 6 27 2 24 19 0.0 6 18 1 25 11 0.0 6 10 0 0 0 0 1 26 13 0.0 6 11 2 27 6 0.0 6 6 28 8 0.0 6 8 29 9 0.0 6 8 1 30 4 0.0 6 4 31 8 0.0 6 8 32 5 0.0 6 4 0 0 0 0 0 1 33 9 0.0 6 8 1 34 6 0.0 6 6 35 5 0.0 6 4 1 36 3 0.0 6 3 37 2 0.0 6 2 38 2 0.0 6 2 39 7 0.0 6 7 40 2 0.0 6 2 41 8 0.0 6 5 2 1 42 5 0.0 6 4 1 43 2 0.0 6 1 1 44 6 0.0 6 6 45 5 0.0 6 5 46 6 0.0 6 6 47 3 0.0 6 3 48 2 0.0 6 2 49 4 0.0 6 4 50 6 0.0 6 6 51 1 0.0 6 1 52 4 0.0 6 4 53 2 0.0 6 2 54 3 0.0 6 3 55 3 0.0 6 3 56 2 0.0 6 2 57 2 0.0 6 1 0 0 1 59 3 0.0 6 2 0 1 60 5 0.0 6 5 61 1 0.0 6 1 62 4 0.0 6 3 1 63 2 0.0 6 2 64 3 0.0 6 3 65 6 0.0 6 5 1 66 9 0.0 6 9 67 8 0.0 6 7 1 68 6 0.0 6 6 69 4 0.0 6 4 70 6 0.0 6 5 1 71 6 0.0 6 6 72 12 0.0 6 11 1 73 2 0.0 6 1 0 0 0 0 1 74 4 0.0 6 4 75 5 0.0 6 5 76 4 0.0 6 4 78 5 0.0 6 4 0 1 79 6 0.0 6 5 0 0 0 1 80 6 0.0 6 5 1 81 6 0.0 6 6 82 4 0.0 6 4 83 10 0.0 6 10 84 9 0.0 6 9 85 10 0.0 6 10 86 8 0.0 6 8 87 6 0.0 6 6 88 6 0.0 6 6 89 6 0.0 6 6 90 6 0.0 6 6 91 8 0.0 6 8 92 4 0.0 6 4 93 7 0.0 6 7 94 9 0.0 6 8 1 95 7 0.0 6 7 96 10 0.0 6 9 1 97 4 0.0 6 4 98 9 0.0 6 9 99 11 0.0 6 11 100 8 0.0 6 7 0 0 0 0 0 1 101 10 0.0 6 10 102 20 0.0 6 20 103 11 0.0 6 11 104 10 0.0 6 9 1 105 17 0.0 6 17 106 18 0.0 6 17 0 1 107 11 0.0 6 10 1 108 14 0.0 6 14 109 19 0.0 6 19 110 14 0.0 6 13 1 111 17 0.0 6 17 112 25 0.0 6 25 113 20 0.0 6 19 1 114 16 0.0 6 15 1 115 26 0.0 6 25 1 116 24 0.0 6 24 117 17 0.0 6 11 1 0 0 0 5 118 16 0.0 6 15 1 119 27 0.0 6 27 120 18 0.0 6 18 121 19 0.0 6 19 122 20 0.0 6 20 123 20 0.0 6 20 124 22 0.0 6 22 125 22 0.0 6 21 1 126 24 0.0 6 24 127 24 0.0 6 24 128 25 0.0 6 25 129 37 0.0 6 37 130 32 0.0 6 32 131 35 0.0 6 34 1 132 33 0.0 6 33 133 32 0.0 6 30 1 0 1 134 30 0.0 6 30 135 42 0.0 6 42 136 42 0.0 6 41 1 137 34 0.0 6 34 138 42 0.0 6 42 139 56 0.0 6 56 140 126 0.0 6 125 1 141 256 0.0 6 256 142 321 0.0 6 320 1 143 354 0.0 6 352 2 144 331 0.0 6 326 5 145 426 0.0 6 424 2 146 481 0.0 6 481 147 368 0.0 6 367 1 148 435 0.0 6 434 1 149 405 0.0 6 404 1 150 488 0.0 6 487 1 151 336 0.0 6 335 1 152 250 0.0 6 250 153 197 0.0 6 197 154 284 0.0 6 283 1 155 285 0.0 6 285 156 260 0.0 6 259 1 157 265 0.0 6 264 1 158 292 0.0 6 292 159 345 0.0 6 345 160 324 0.0 6 324 161 309 0.0 6 309 162 290 0.0 6 290 163 278 0.0 6 277 1 164 301 0.0 6 299 2 165 242 0.0 6 241 1 166 218 0.0 6 217 1 167 250 0.0 6 250 168 270 0.0 6 269 1 169 323 0.0 6 323 170 291 0.0 6 289 2 171 277 0.0 6 277 172 313 0.0 6 312 1 173 256 0.0 6 255 1 174 198 0.0 6 198 175 287 0.0 6 287 176 293 0.0 6 293 177 266 0.0 6 266 178 546 0.0 6 546 179 415 0.0 6 415 180 355 0.0 6 353 2 181 378 0.0 6 376 2 182 303 0.0 6 302 1 183 340 0.0 6 340 184 298 0.0 6 298 185 358 0.0 6 358 186 325 0.0 6 324 0 0 0 0 0 1 187 346 0.0 6 345 1 188 326 0.0 6 326 189 306 0.0 6 304 2 190 333 0.0 6 332 1 191 362 0.0 6 361 1 192 384 0.0 6 383 1 193 483 0.0 6 482 0 1 194 446 0.0 6 441 5 195 445 0.0 6 441 4 196 620 0.0 6 617 3 197 578 0.0 6 577 1 198 539 0.0 6 535 3 0 0 0 0 1 199 533 0.0 6 529 4 200 432 0.0 6 426 6 201 552 0.0 6 550 2 202 561 0.0 6 558 2 1 203 567 0.0 6 564 2 0 0 0 0 1 204 494 0.0 6 493 1 205 533 0.0 6 529 4 206 646 0.0 6 644 2 207 552 0.0 6 547 5 208 642 0.0 6 642 209 501 0.0 6 500 1 210 466 0.0 6 462 4 211 577 0.0 6 574 3 212 546 0.0 6 545 1 213 536 0.0 6 534 2 214 655 0.0 6 652 3 215 731 0.0 6 727 4 216 519 0.0 6 518 1 217 528 0.0 6 525 3 218 651 0.0 6 647 3 0 1 219 556 0.0 6 553 3 220 462 0.0 6 462 221 450 0.0 6 448 2 222 463 0.0 6 461 1 0 0 0 0 1 223 525 0.0 6 522 3 224 479 0.0 6 479 225 524 0.0 6 523 0 0 1 226 512 0.0 6 504 7 0 1 227 485 0.0 6 484 1 228 423 0.0 6 422 1 229 396 0.0 6 394 2 230 442 0.0 6 440 2 231 423 0.0 6 422 1 232 399 0.0 6 398 1 233 381 0.0 6 379 2 234 345 0.0 6 344 1 235 324 0.0 6 324 236 321 0.0 6 320 1 237 318 0.0 6 318 238 350 0.0 6 350 239 308 0.0 6 306 1 0 0 0 1 240 324 0.0 6 320 2 1 0 1 241 353 0.0 6 351 2 242 332 0.0 6 328 4 243 307 0.0 6 305 2 244 266 0.0 6 264 1 0 0 0 1 245 228 0.0 6 228 246 211 0.0 6 211 247 190 0.0 6 190 248 215 0.0 6 215 249 173 0.0 6 173 250 201 0.0 6 201 251 198 0.0 6 196 2 252 196 0.0 6 194 1 1 253 169 0.0 6 169 254 128 0.0 6 128 255 170 0.0 6 169 1 256 150 0.0 6 149 1 257 159 0.0 6 158 1 258 158 0.0 6 157 1 259 164 0.0 6 164 260 158 0.0 6 156 2 261 130 0.0 6 129 1 262 113 0.0 6 112 1 263 114 0.0 6 113 1 264 134 0.0 6 134 265 97 0.0 6 97 266 115 0.0 6 114 1 267 95 0.0 6 95 268 120 0.0 6 117 3 269 120 0.0 6 118 2 270 103 0.0 6 103 271 93 0.0 6 93 272 91 0.0 6 90 1 273 204 0.0 6 203 1 274 138 0.0 6 137 1 275 173 0.0 6 172 1 276 168 0.0 6 167 1 277 251 0.0 6 248 3 278 1234 0.0 6 1223 11 279 1183 0.0 6 1167 16 280 12 0.0 6 10 2 281 4 0.0 6 4 283 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1076 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 24 0.0 6 24 71 1 0.0 6 1 72 1 0.0 6 1 73 8 0.0 6 8 74 12 0.0 6 12 75 12 0.0 6 12 76 7 0.0 6 7 77 16 0.0 6 16 78 16 0.0 6 16 79 8 0.0 6 8 80 8 0.0 6 8 81 9 0.0 6 9 82 11 0.0 6 11 83 7 0.0 6 7 84 5 0.0 6 5 85 8 0.0 6 8 86 3 0.0 6 3 87 1 0.0 6 1 88 7 0.0 6 7 89 9 0.0 6 9 90 6 0.0 6 6 91 13 0.0 6 13 92 12 0.0 6 11 1 93 9 0.0 6 9 94 6 0.0 6 6 95 4 0.0 6 4 96 6 0.0 6 6 97 8 0.0 6 8 98 4 0.0 6 4 99 4 0.0 6 4 100 3 0.0 6 3 101 6 0.0 6 6 102 9 0.0 6 9 103 6 0.0 6 6 104 5 0.0 6 5 105 1 0.0 6 1 106 3 0.0 6 3 107 9 0.0 6 9 108 7 0.0 6 7 109 8 0.0 6 8 110 20 0.0 6 20 111 3 0.0 6 3 112 10 0.0 6 10 113 9 0.0 6 9 114 4 0.0 6 4 115 7 0.0 6 7 116 6 0.0 6 6 117 6 0.0 6 6 118 4 0.0 6 4 119 4 0.0 6 4 120 5 0.0 6 5 121 2 0.0 6 2 122 2 0.0 6 2 123 9 0.0 6 9 124 6 0.0 6 6 125 10 0.0 6 10 126 5 0.0 6 5 127 6 0.0 6 6 128 6 0.0 6 6 129 5 0.0 6 5 130 8 0.0 6 8 131 6 0.0 6 6 132 2 0.0 6 2 133 10 0.0 6 10 134 14 0.0 6 14 135 10 0.0 6 10 136 6 0.0 6 6 137 6 0.0 6 6 138 4 0.0 6 4 139 6 0.0 6 6 140 14 0.0 6 14 141 7 0.0 6 7 142 6 0.0 6 6 143 2 0.0 6 2 144 6 0.0 6 6 145 5 0.0 6 5 146 10 0.0 6 10 147 8 0.0 6 8 148 10 0.0 6 10 149 8 0.0 6 8 150 4 0.0 6 4 151 3 0.0 6 3 152 7 0.0 6 7 153 5 0.0 6 5 154 5 0.0 6 5 155 6 0.0 6 6 156 3 0.0 6 3 157 8 0.0 6 8 158 8 0.0 6 8 159 3 0.0 6 3 160 5 0.0 6 5 161 3 0.0 6 3 162 9 0.0 6 9 163 5 0.0 6 5 164 6 0.0 6 6 165 8 0.0 6 8 166 6 0.0 6 6 167 6 0.0 6 6 168 7 0.0 6 7 169 9 0.0 6 9 170 10 0.0 6 10 171 12 0.0 6 12 172 6 0.0 6 6 173 9 0.0 6 9 174 11 0.0 6 11 175 7 0.0 6 7 176 6 0.0 6 6 177 2 0.0 6 2 178 6 0.0 6 6 179 7 0.0 6 7 180 3 0.0 6 3 181 7 0.0 6 7 182 12 0.0 6 12 183 6 0.0 6 6 184 8 0.0 6 8 185 6 0.0 6 6 186 4 0.0 6 4 187 6 0.0 6 6 188 9 0.0 6 9 189 6 0.0 6 6 190 6 0.0 6 6 191 3 0.0 6 3 192 4 0.0 6 4 193 7 0.0 6 7 194 2 0.0 6 2 195 5 0.0 6 5 196 3 0.0 6 3 197 3 0.0 6 3 198 3 0.0 6 3 199 6 0.0 6 6 200 4 0.0 6 4 201 3 0.0 6 3 202 6 0.0 6 6 203 5 0.0 6 5 204 6 0.0 6 6 205 6 0.0 6 6 206 2 0.0 6 2 207 1 0.0 6 1 208 8 0.0 6 8 209 4 0.0 6 3 1 210 5 0.0 6 5 211 5 0.0 6 5 212 5 0.0 6 5 213 7 0.0 6 7 214 2 0.0 6 2 215 2 0.0 6 2 216 5 0.0 6 5 217 3 0.0 6 3 218 3 0.0 6 3 219 5 0.0 6 5 220 4 0.0 6 4 221 3 0.0 6 3 222 2 0.0 6 2 223 6 0.0 6 6 224 5 0.0 6 5 225 7 0.0 6 7 226 6 0.0 6 6 227 3 0.0 6 3 228 3 0.0 6 3 229 1 0.0 6 1 230 4 0.0 6 4 231 1 0.0 6 1 232 4 0.0 6 4 233 3 0.0 6 3 235 5 0.0 6 5 236 6 0.0 6 6 238 1 0.0 6 1 239 1 0.0 6 1 240 1 0.0 6 1 242 1 0.0 6 1 243 2 0.0 6 2 244 2 0.0 6 2 245 1 0.0 6 1 246 3 0.0 6 3 247 5 0.0 6 5 248 3 0.0 6 3 250 1 0.0 6 1 251 1 0.0 6 1 253 2 0.0 6 2 254 2 0.0 6 2 255 1 0.0 6 1 256 2 0.0 6 1 1 258 3 0.0 6 3 260 2 0.0 6 2 262 1 0.0 6 1 263 1 0.0 6 1 264 1 0.0 6 1 265 1 0.0 6 1 266 1 0.0 6 1 275 1 0.0 6 1 283 1 0.0 6 1 292 1 0.0 6 1 293 1 0.0 6 1 295 1 0.0 6 1 298 1 0.0 6 1 311 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 50818 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 1 3.2 2 1 9 1 0.2 2 0 1 10 1 0.0 3 1 11 2 0.0 3 2 12 4 0.0 3 4 13 4 0.0 3 3 0 0 1 14 4 0.0 4 3 1 16 4 0.0 4 3 1 17 9 0.0 5 9 18 1 0.0 5 1 19 17 0.0 5 17 20 44 0.0 6 44 21 20 0.0 6 19 1 22 17 0.0 6 17 23 17 0.0 6 16 0 0 1 24 6 0.0 6 6 25 8 0.0 6 7 1 26 9 0.0 6 8 0 1 27 5 0.0 6 3 2 28 8 0.0 6 8 29 6 0.0 6 6 30 8 0.0 6 7 1 31 8 0.0 6 8 32 6 0.0 6 6 33 3 0.0 6 3 34 4 0.0 6 4 35 7 0.0 6 7 36 4 0.0 6 4 37 12 0.0 6 12 38 10 0.0 6 10 39 6 0.0 6 5 1 40 14 0.0 6 12 2 41 4 0.0 6 2 2 42 4 0.0 6 4 43 7 0.0 6 7 44 2 0.0 6 2 45 5 0.0 6 4 1 46 9 0.0 6 8 0 0 0 0 0 1 47 12 0.0 6 11 0 0 0 0 0 1 48 4 0.0 6 3 1 49 9 0.0 6 9 50 6 0.0 6 5 1 51 8 0.0 6 7 1 52 6 0.0 6 6 53 17 0.0 6 17 54 12 0.0 6 12 55 13 0.0 6 12 0 0 1 56 16 0.0 6 16 57 8 0.0 6 8 58 8 0.0 6 8 59 9 0.0 6 8 1 60 7 0.0 6 6 1 61 13 0.0 6 13 62 16 0.0 6 15 1 63 12 0.0 6 11 1 64 10 0.0 6 9 1 65 15 0.0 6 15 66 16 0.0 6 15 1 67 25 0.0 6 23 2 68 22 0.0 6 21 1 69 27 0.0 6 24 1 1 1 70 116 0.0 6 109 3 1 1 1 0 1 71 32 0.0 6 31 0 1 72 39 0.0 6 36 3 73 27 0.0 6 26 0 0 1 74 41 0.0 6 38 1 0 0 0 0 2 75 26 0.0 6 23 1 1 0 1 76 20 0.0 6 19 1 77 27 0.0 6 26 1 78 23 0.0 6 23 79 19 0.0 6 19 80 16 0.0 6 14 1 0 0 0 1 81 22 0.0 6 21 0 0 0 0 0 1 82 34 0.0 6 31 1 1 1 83 26 0.0 6 26 84 27 0.0 6 23 4 85 28 0.0 6 24 3 0 0 1 86 49 0.0 6 48 1 87 24 0.0 6 23 0 0 0 1 88 30 0.0 6 28 2 89 44 0.0 6 42 2 90 22 0.0 6 22 91 29 0.0 6 28 1 92 31 0.0 6 28 2 0 1 93 42 0.0 6 39 3 94 32 0.0 6 31 1 95 45 0.0 6 45 96 53 0.0 6 52 1 97 41 0.0 6 38 3 98 48 0.0 6 46 1 1 99 51 0.0 6 49 2 100 49 0.0 6 48 1 101 45 0.0 6 42 2 0 0 0 1 102 42 0.0 6 42 103 53 0.0 6 52 1 104 61 0.0 6 59 1 0 1 105 60 0.0 6 60 106 73 0.0 6 69 3 0 0 0 0 1 107 99 0.0 6 97 0 1 0 0 1 108 50 0.0 6 49 1 109 65 0.0 6 61 3 0 0 0 0 1 110 60 0.0 6 58 1 0 1 111 65 0.0 6 63 1 0 1 112 73 0.0 6 72 0 0 0 0 1 113 95 0.0 6 92 1 1 0 1 114 84 0.0 6 82 2 115 106 0.0 6 104 1 0 1 116 92 0.0 6 90 2 117 88 0.0 6 88 118 104 0.0 6 99 3 0 1 0 0 1 119 96 0.0 6 92 3 0 0 0 0 1 120 134 0.0 6 132 1 0 0 1 121 127 0.0 6 121 5 0 0 0 0 1 122 128 0.0 6 126 1 1 123 97 0.0 6 96 1 124 128 0.0 6 125 2 0 0 0 1 125 126 0.0 6 124 2 126 140 0.0 6 133 5 0 0 0 0 2 127 174 0.0 6 172 1 0 1 128 192 0.0 6 191 1 129 174 0.0 6 173 1 130 202 0.0 6 199 2 0 1 131 236 0.0 6 235 1 132 244 0.0 6 240 2 1 0 1 133 237 0.0 6 233 4 134 259 0.0 6 259 135 266 0.0 6 264 1 0 1 136 284 0.0 6 282 2 137 338 0.0 6 332 5 0 0 0 0 1 138 320 0.0 6 315 3 0 0 1 0 1 139 330 0.0 6 326 4 140 410 0.0 6 407 3 141 510 0.0 6 505 4 0 1 142 600 0.0 6 594 4 0 0 1 0 1 143 666 0.0 6 659 6 0 1 144 635 0.0 6 627 8 145 751 0.0 6 740 9 0 1 0 0 1 146 764 0.0 6 760 4 147 762 0.0 6 759 3 148 789 0.0 6 783 5 0 1 149 779 0.0 6 768 9 1 0 0 1 150 935 0.0 6 927 7 0 1 151 873 0.0 6 862 7 2 0 0 2 152 876 0.0 6 866 9 0 1 153 1113 0.0 6 1104 7 1 1 154 910 0.0 6 901 7 2 155 1076 0.0 6 1068 7 0 0 1 156 1030 0.0 6 1017 11 0 0 1 0 1 157 810 0.0 6 803 5 0 0 2 158 914 0.0 6 909 5 159 803 0.0 6 802 0 0 0 0 1 160 926 0.0 6 916 7 1 1 1 161 868 0.0 6 863 4 0 0 0 0 1 162 839 0.0 6 831 5 1 0 2 163 806 0.0 6 799 6 0 0 1 164 748 0.0 6 741 6 0 0 1 165 690 0.0 6 684 6 166 765 0.0 6 757 5 0 0 1 1 1 167 640 0.0 6 635 5 168 670 0.0 6 663 6 1 169 800 0.0 6 792 7 0 0 0 1 170 705 0.0 6 698 6 1 171 817 0.0 6 812 2 0 1 0 2 172 669 0.0 6 663 4 0 0 0 0 2 173 599 0.0 6 594 2 2 1 174 710 0.0 6 707 3 175 749 0.0 6 743 6 176 810 0.0 6 803 5 1 1 177 704 0.0 6 697 6 0 0 1 178 708 0.0 6 704 4 179 726 0.0 6 724 2 180 532 0.0 6 531 1 181 649 0.0 6 644 5 182 442 0.0 6 437 5 183 448 0.0 6 442 6 184 359 0.0 6 357 2 185 370 0.0 6 365 2 1 1 0 1 186 615 0.0 6 609 3 1 0 1 0 1 187 749 0.0 6 744 3 1 0 1 188 646 0.0 6 639 6 0 0 0 1 189 444 0.0 6 442 2 190 477 0.0 6 471 5 1 191 494 0.0 6 489 5 192 398 0.0 6 393 5 193 347 0.0 6 345 2 194 417 0.0 6 409 7 1 195 325 0.0 6 320 5 196 405 0.0 6 398 7 197 271 0.0 6 268 2 0 0 1 198 263 0.0 6 259 4 199 449 0.0 6 444 4 0 0 1 200 260 0.0 6 257 3 201 264 0.0 6 259 5 202 216 0.0 6 212 4 203 191 0.0 6 188 3 204 142 0.0 6 140 2 205 134 0.0 6 131 3 206 154 0.0 6 153 1 207 127 0.0 6 126 1 208 140 0.0 6 135 4 0 1 209 128 0.0 6 126 1 1 210 105 0.0 6 102 1 1 0 0 1 211 116 0.0 6 115 1 212 102 0.0 6 101 1 213 76 0.0 6 73 3 214 107 0.0 6 106 1 215 96 0.0 6 94 1 1 216 71 0.0 6 70 1 217 110 0.0 6 110 218 77 0.0 6 77 219 61 0.0 6 61 220 64 0.0 6 63 1 221 63 0.0 6 63 222 41 0.0 6 39 2 223 38 0.0 6 35 3 224 58 0.0 6 58 225 34 0.0 6 32 2 226 58 0.0 6 57 1 227 31 0.0 6 29 2 228 28 0.0 6 28 229 28 0.0 6 28 230 41 0.0 6 40 1 231 28 0.0 6 28 232 27 0.0 6 26 1 233 40 0.0 6 38 2 234 39 0.0 6 39 235 19 0.0 6 18 1 236 5 0.0 6 5 237 9 0.0 6 9 238 7 0.0 6 7 239 3 0.0 6 3 240 4 0.0 6 4 241 9 0.0 6 9 242 6 0.0 6 6 243 17 0.0 6 17 244 8 0.0 6 8 245 5 0.0 6 5 246 7 0.0 6 6 1 247 9 0.0 6 9 248 3 0.0 6 3 249 9 0.0 6 9 250 3 0.0 6 3 251 6 0.0 6 6 252 2 0.0 6 2 254 5 0.0 6 5 255 3 0.0 6 3 256 1 0.0 6 1 280 3 0.0 6 3 281 9 0.0 6 9 282 63 0.0 6 62 1 283 1 0.0 6 0 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1020 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 5 0.0 6 0 5 57 1 0.0 6 0 0 0 0 1 68 2 0.0 6 2 69 3 0.0 6 3 70 12 0.0 6 12 71 12 0.0 6 12 72 11 0.0 6 11 73 9 0.0 6 9 74 19 0.0 6 19 75 15 0.0 6 15 76 13 0.0 6 13 77 15 0.0 6 15 78 14 0.0 6 14 79 13 0.0 6 13 80 13 0.0 6 13 81 4 0.0 6 4 82 6 0.0 6 6 83 8 0.0 6 8 84 12 0.0 6 12 85 4 0.0 6 4 86 3 0.0 6 3 87 8 0.0 6 8 88 15 0.0 6 15 89 10 0.0 6 10 90 8 0.0 6 8 91 4 0.0 6 4 92 11 0.0 6 11 93 8 0.0 6 8 94 8 0.0 6 8 95 9 0.0 6 9 96 1 0.0 6 1 97 4 0.0 6 4 98 6 0.0 6 6 99 6 0.0 6 6 100 7 0.0 6 7 101 4 0.0 6 4 102 3 0.0 6 3 103 3 0.0 6 2 0 0 1 104 3 0.0 6 3 105 2 0.0 6 2 106 7 0.0 6 7 107 15 0.0 6 15 108 11 0.0 6 11 109 9 0.0 6 9 110 5 0.0 6 5 111 5 0.0 6 5 112 6 0.0 6 6 113 5 0.0 6 5 114 7 0.0 6 7 115 4 0.0 6 4 116 9 0.0 6 9 117 8 0.0 6 8 118 5 0.0 6 5 119 6 0.0 6 6 120 7 0.0 6 7 121 6 0.0 6 6 122 8 0.0 6 8 123 5 0.0 6 5 124 4 0.0 6 4 125 8 0.0 6 8 126 8 0.0 6 8 127 5 0.0 6 5 128 10 0.0 6 10 129 7 0.0 6 6 1 130 9 0.0 6 9 131 16 0.0 6 16 132 9 0.0 6 8 1 133 8 0.0 6 8 134 4 0.0 6 4 135 2 0.0 6 2 136 9 0.0 6 8 1 137 18 0.0 6 18 138 10 0.0 6 10 139 6 0.0 6 6 140 10 0.0 6 10 141 5 0.0 6 5 142 7 0.0 6 7 143 7 0.0 6 7 144 11 0.0 6 10 1 145 9 0.0 6 8 1 146 7 0.0 6 6 1 147 12 0.0 6 11 1 148 5 0.0 6 5 149 4 0.0 6 4 150 8 0.0 6 8 151 15 0.0 6 15 152 10 0.0 6 10 153 7 0.0 6 7 154 4 0.0 6 4 155 9 0.0 6 9 156 7 0.0 6 7 157 4 0.0 6 4 158 5 0.0 6 5 159 9 0.0 6 9 160 3 0.0 6 3 161 9 0.0 6 9 162 7 0.0 6 7 163 4 0.0 6 4 164 10 0.0 6 10 165 11 0.0 6 11 166 16 0.0 6 16 167 5 0.0 6 5 168 9 0.0 6 9 169 5 0.0 6 5 170 10 0.0 6 10 171 11 0.0 6 11 172 6 0.0 6 6 173 14 0.0 6 14 174 7 0.0 6 7 175 4 0.0 6 4 176 6 0.0 6 6 177 6 0.0 6 6 178 4 0.0 6 4 179 8 0.0 6 8 180 9 0.0 6 9 181 5 0.0 6 5 182 4 0.0 6 3 0 0 0 1 183 5 0.0 6 5 184 7 0.0 6 7 185 6 0.0 6 6 186 7 0.0 6 7 187 4 0.0 6 3 1 188 4 0.0 6 4 189 6 0.0 6 6 190 7 0.0 6 7 191 5 0.0 6 5 192 3 0.0 6 3 193 5 0.0 6 5 194 4 0.0 6 4 195 1 0.0 6 1 196 3 0.0 6 3 197 3 0.0 6 3 198 2 0.0 6 2 199 2 0.0 6 2 200 2 0.0 6 2 201 7 0.0 6 7 202 2 0.0 6 2 203 2 0.0 6 2 204 5 0.0 6 5 205 2 0.0 6 2 206 1 0.0 6 1 207 2 0.0 6 2 208 2 0.0 6 2 210 3 0.0 6 3 211 1 0.0 6 1 212 2 0.0 6 2 213 1 0.0 6 1 214 1 0.0 6 1 215 3 0.0 6 3 216 1 0.0 6 1 217 1 0.0 6 1 219 1 0.0 6 1 225 2 0.0 6 2 233 1 0.0 6 1 234 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_12_197_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_12_424_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_12_197_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_12_424_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.98 s (133 µs/read; 0.45 M reads/minute). === Summary === Total read pairs processed: 37,402 Read 1 with adapter: 37,396 (100.0%) Read 2 with adapter: 37,354 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 54 (0.1%) Pairs written (passing filters): 37,348 (99.9%) Total basepairs processed: 18,279,114 bp Read 1: 10,365,268 bp Read 2: 7,913,846 bp Total written (filtered): 2,355,133 bp (12.9%) Read 1: 1,196,733 bp Read 2: 1,158,400 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 37366 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 146.1 1 1 6 2 9.1 1 0 0 2 7 1 2.3 2 1 14 1 0.0 4 0 1 16 1 0.0 4 1 19 1 0.0 5 0 1 20 6 0.0 6 6 21 3 0.0 6 3 22 3 0.0 6 3 23 1 0.0 6 1 25 5 0.0 6 5 26 3 0.0 6 2 1 27 3 0.0 6 3 28 2 0.0 6 1 1 30 1 0.0 6 1 33 1 0.0 6 1 37 2 0.0 6 1 1 38 1 0.0 6 1 39 1 0.0 6 0 0 1 40 3 0.0 6 2 0 1 42 1 0.0 6 1 44 3 0.0 6 2 1 45 4 0.0 6 4 46 1 0.0 6 1 47 2 0.0 6 2 49 3 0.0 6 3 52 2 0.0 6 2 53 3 0.0 6 3 54 3 0.0 6 3 55 1 0.0 6 0 1 56 4 0.0 6 3 1 57 2 0.0 6 2 58 1 0.0 6 1 59 1 0.0 6 1 60 2 0.0 6 1 1 62 3 0.0 6 3 63 1 0.0 6 1 67 3 0.0 6 3 68 1 0.0 6 1 69 3 0.0 6 2 0 1 70 1 0.0 6 1 71 1 0.0 6 1 72 1 0.0 6 1 73 2 0.0 6 1 1 75 1 0.0 6 0 1 77 3 0.0 6 3 78 1 0.0 6 1 79 1 0.0 6 1 81 3 0.0 6 3 83 1 0.0 6 1 86 8 0.0 6 8 87 1 0.0 6 1 89 3 0.0 6 3 90 3 0.0 6 3 91 1 0.0 6 1 92 2 0.0 6 2 95 1 0.0 6 1 97 7 0.0 6 6 0 1 98 8 0.0 6 6 2 99 4 0.0 6 4 100 2 0.0 6 1 1 101 2 0.0 6 2 102 5 0.0 6 5 103 3 0.0 6 3 104 2 0.0 6 2 105 4 0.0 6 4 106 5 0.0 6 5 107 2 0.0 6 2 108 8 0.0 6 8 109 5 0.0 6 5 110 1 0.0 6 1 111 3 0.0 6 3 112 11 0.0 6 10 1 113 4 0.0 6 4 114 2 0.0 6 2 115 5 0.0 6 4 1 116 3 0.0 6 3 117 2 0.0 6 2 118 3 0.0 6 3 119 4 0.0 6 4 120 2 0.0 6 2 121 9 0.0 6 8 1 122 13 0.0 6 12 1 123 5 0.0 6 5 124 9 0.0 6 7 2 125 8 0.0 6 7 1 126 5 0.0 6 5 127 5 0.0 6 3 2 128 14 0.0 6 14 129 11 0.0 6 11 130 5 0.0 6 4 1 131 4 0.0 6 4 132 5 0.0 6 5 133 8 0.0 6 8 134 8 0.0 6 8 135 13 0.0 6 13 136 10 0.0 6 9 1 137 23 0.0 6 22 1 138 21 0.0 6 21 139 7 0.0 6 5 2 140 30 0.0 6 29 1 141 21 0.0 6 20 1 142 63 0.0 6 63 143 37 0.0 6 36 0 1 144 18 0.0 6 17 1 145 63 0.0 6 59 4 146 72 0.0 6 70 2 147 39 0.0 6 39 148 47 0.0 6 46 1 149 68 0.0 6 67 1 150 64 0.0 6 63 1 151 61 0.0 6 60 1 152 52 0.0 6 52 153 31 0.0 6 30 1 154 48 0.0 6 48 155 40 0.0 6 40 156 18 0.0 6 18 157 32 0.0 6 30 2 158 23 0.0 6 23 159 21 0.0 6 21 160 77 0.0 6 77 161 33 0.0 6 30 3 162 22 0.0 6 22 163 28 0.0 6 26 2 164 54 0.0 6 54 165 26 0.0 6 25 1 166 23 0.0 6 21 1 0 1 167 38 0.0 6 37 1 168 36 0.0 6 36 169 37 0.0 6 34 3 170 33 0.0 6 32 1 171 31 0.0 6 31 172 42 0.0 6 42 173 35 0.0 6 32 3 174 35 0.0 6 33 2 175 44 0.0 6 43 1 176 47 0.0 6 46 1 177 33 0.0 6 32 1 178 38 0.0 6 38 179 78 0.0 6 76 2 180 53 0.0 6 52 1 181 51 0.0 6 50 1 182 82 0.0 6 77 5 183 51 0.0 6 48 3 184 72 0.0 6 72 185 97 0.0 6 92 4 0 0 1 186 99 0.0 6 98 1 187 71 0.0 6 69 2 188 82 0.0 6 79 3 189 63 0.0 6 63 190 96 0.0 6 93 3 191 106 0.0 6 102 4 192 110 0.0 6 107 3 193 155 0.0 6 152 3 194 123 0.0 6 115 8 195 160 0.0 6 149 11 196 158 0.0 6 151 6 1 197 191 0.0 6 187 4 198 213 0.0 6 208 4 1 199 161 0.0 6 159 2 200 226 0.0 6 219 7 201 193 0.0 6 190 3 202 198 0.0 6 188 10 203 225 0.0 6 219 6 204 188 0.0 6 182 6 205 249 0.0 6 239 9 1 206 223 0.0 6 219 4 207 242 0.0 6 234 8 208 227 0.0 6 220 7 209 208 0.0 6 203 5 210 132 0.0 6 128 4 211 113 0.0 6 111 2 212 131 0.0 6 126 5 213 179 0.0 6 174 5 214 240 0.0 6 231 9 215 164 0.0 6 162 2 216 158 0.0 6 153 5 217 202 0.0 6 199 3 218 193 0.0 6 185 8 219 198 0.0 6 194 4 220 117 0.0 6 115 2 221 145 0.0 6 143 2 222 272 0.0 6 267 5 223 244 0.0 6 240 3 0 0 0 1 224 163 0.0 6 159 4 225 229 0.0 6 227 2 226 554 0.0 6 545 9 227 347 0.0 6 339 8 228 306 0.0 6 297 9 229 232 0.0 6 227 4 1 230 291 0.0 6 286 5 231 188 0.0 6 186 2 232 278 0.0 6 272 6 233 216 0.0 6 211 5 234 230 0.0 6 227 3 235 180 0.0 6 177 3 236 171 0.0 6 168 3 237 200 0.0 6 192 7 1 238 323 0.0 6 319 4 239 300 0.0 6 291 9 240 357 0.0 6 350 7 241 400 0.0 6 391 9 242 470 0.0 6 464 6 243 325 0.0 6 318 7 244 230 0.0 6 224 6 245 411 0.0 6 406 5 246 225 0.0 6 220 5 247 216 0.0 6 213 3 248 177 0.0 6 171 6 249 385 0.0 6 377 8 250 285 0.0 6 279 6 251 380 0.0 6 378 2 252 231 0.0 6 229 2 253 238 0.0 6 234 4 254 567 0.0 6 557 10 255 394 0.0 6 387 7 256 358 0.0 6 352 6 257 410 0.0 6 404 6 258 444 0.0 6 437 6 1 259 531 0.0 6 517 13 0 1 260 659 0.0 6 650 9 261 231 0.0 6 227 4 262 218 0.0 6 206 12 263 507 0.0 6 500 7 264 176 0.0 6 171 5 265 338 0.0 6 332 6 266 285 0.0 6 278 7 267 321 0.0 6 314 7 268 352 0.0 6 341 10 1 269 590 0.0 6 583 7 270 302 0.0 6 296 6 271 287 0.0 6 279 8 272 587 0.0 6 571 15 1 273 585 0.0 6 568 17 274 792 0.0 6 778 13 1 275 567 0.0 6 552 15 276 773 0.0 6 749 24 277 1393 0.0 6 1372 20 1 278 3641 0.0 6 3563 77 1 279 4560 0.0 6 4413 143 3 0 1 280 52 0.0 6 47 4 1 281 9 0.0 6 7 1 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 30 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 3 0.0 6 3 74 1 0.0 6 1 75 2 0.0 6 2 76 2 0.0 6 2 78 1 0.0 6 1 79 1 0.0 6 1 80 2 0.0 6 2 81 2 0.0 6 2 82 1 0.0 6 1 84 2 0.0 6 2 85 1 0.0 6 1 87 1 0.0 6 1 89 1 0.0 6 1 90 1 0.0 6 1 92 1 0.0 6 1 96 1 0.0 6 1 98 1 0.0 6 1 108 2 0.0 6 2 114 1 0.0 6 1 124 1 0.0 6 1 130 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 37336 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 584.4 0 0 3 9 1 0.1 2 0 1 10 1 0.0 3 1 11 1 0.0 3 1 12 3 0.0 3 3 13 6 0.0 3 4 2 14 6 0.0 4 6 16 2 0.0 4 1 1 17 12 0.0 5 12 18 8 0.0 5 7 1 19 2 0.0 5 1 1 20 9 0.0 6 9 21 27 0.0 6 26 1 22 20 0.0 6 19 0 1 23 19 0.0 6 19 24 9 0.0 6 9 25 13 0.0 6 12 1 26 7 0.0 6 6 1 27 6 0.0 6 5 1 28 4 0.0 6 4 29 7 0.0 6 7 30 6 0.0 6 5 1 31 1 0.0 6 1 32 2 0.0 6 2 33 3 0.0 6 3 34 4 0.0 6 4 35 8 0.0 6 7 1 36 1 0.0 6 1 37 5 0.0 6 4 1 38 6 0.0 6 5 1 39 7 0.0 6 7 40 7 0.0 6 7 41 2 0.0 6 2 43 6 0.0 6 5 1 44 13 0.0 6 13 45 7 0.0 6 6 0 1 46 10 0.0 6 9 1 47 9 0.0 6 8 1 48 6 0.0 6 6 49 16 0.0 6 13 2 1 50 11 0.0 6 10 1 51 8 0.0 6 6 2 52 16 0.0 6 16 53 9 0.0 6 8 1 54 16 0.0 6 15 1 55 15 0.0 6 14 1 56 6 0.0 6 4 2 57 18 0.0 6 16 2 58 8 0.0 6 8 59 3 0.0 6 3 60 10 0.0 6 10 61 3 0.0 6 3 62 3 0.0 6 2 1 63 2 0.0 6 1 1 64 11 0.0 6 9 2 65 4 0.0 6 3 1 66 5 0.0 6 4 1 67 31 0.0 6 30 0 1 68 66 0.0 6 65 1 69 14 0.0 6 14 70 20 0.0 6 19 1 71 16 0.0 6 15 1 72 35 0.0 6 33 2 73 10 0.0 6 9 1 74 3 0.0 6 2 1 75 10 0.0 6 9 0 1 76 13 0.0 6 12 0 1 77 5 0.0 6 5 78 16 0.0 6 16 79 13 0.0 6 11 2 80 15 0.0 6 13 2 81 8 0.0 6 6 2 82 10 0.0 6 8 2 83 28 0.0 6 27 1 84 36 0.0 6 34 2 85 18 0.0 6 15 2 1 86 17 0.0 6 14 3 87 37 0.0 6 36 1 88 13 0.0 6 12 0 1 89 19 0.0 6 19 90 20 0.0 6 18 2 91 8 0.0 6 7 1 92 9 0.0 6 8 1 93 19 0.0 6 15 4 94 67 0.0 6 65 2 95 18 0.0 6 17 1 96 24 0.0 6 21 2 0 0 1 97 21 0.0 6 19 2 98 17 0.0 6 17 99 29 0.0 6 27 2 100 14 0.0 6 12 2 101 24 0.0 6 23 1 102 41 0.0 6 37 3 0 1 103 44 0.0 6 39 4 1 104 40 0.0 6 39 1 105 69 0.0 6 65 3 0 0 1 106 33 0.0 6 29 3 0 1 107 33 0.0 6 32 1 108 29 0.0 6 27 2 109 21 0.0 6 21 110 33 0.0 6 30 3 111 27 0.0 6 26 1 112 18 0.0 6 17 1 113 23 0.0 6 22 1 114 33 0.0 6 29 4 115 37 0.0 6 34 2 1 116 42 0.0 6 42 117 18 0.0 6 17 1 118 32 0.0 6 28 4 119 44 0.0 6 43 1 120 59 0.0 6 57 2 121 47 0.0 6 42 4 1 122 57 0.0 6 54 3 123 42 0.0 6 39 2 1 124 66 0.0 6 59 6 1 125 44 0.0 6 42 2 126 46 0.0 6 46 127 46 0.0 6 44 2 128 35 0.0 6 31 2 1 0 1 129 27 0.0 6 27 130 42 0.0 6 41 1 131 75 0.0 6 74 1 132 108 0.0 6 107 1 133 75 0.0 6 71 3 0 0 1 134 93 0.0 6 89 4 135 195 0.0 6 190 5 136 81 0.0 6 77 4 137 124 0.0 6 120 4 138 76 0.0 6 74 2 139 91 0.0 6 90 1 140 197 0.0 6 195 2 141 159 0.0 6 151 7 1 142 182 0.0 6 178 4 143 193 0.0 6 189 4 144 287 0.0 6 277 9 1 145 151 0.0 6 147 4 146 157 0.0 6 149 7 1 147 107 0.0 6 103 3 1 148 171 0.0 6 163 6 2 149 274 0.0 6 264 10 150 350 0.0 6 338 11 1 151 215 0.0 6 211 3 0 1 152 373 0.0 6 361 11 0 0 1 153 228 0.0 6 225 3 154 297 0.0 6 292 5 155 181 0.0 6 174 7 156 203 0.0 6 194 8 1 157 282 0.0 6 273 8 0 0 1 158 333 0.0 6 330 2 1 159 164 0.0 6 160 3 0 0 1 160 302 0.0 6 286 15 1 161 168 0.0 6 162 6 162 183 0.0 6 180 2 0 1 163 268 0.0 6 260 8 164 247 0.0 6 242 5 165 296 0.0 6 287 9 166 336 0.0 6 326 9 0 0 1 167 211 0.0 6 209 2 168 447 0.0 6 436 10 0 0 1 169 129 0.0 6 126 2 1 170 151 0.0 6 145 6 171 388 0.0 6 384 3 1 172 167 0.0 6 160 7 173 239 0.0 6 231 8 174 300 0.0 6 295 4 0 1 175 638 0.0 6 626 11 1 176 359 0.0 6 348 9 1 0 1 177 470 0.0 6 456 12 2 178 297 0.0 6 283 14 179 469 0.0 6 460 9 180 387 0.0 6 380 7 181 664 0.0 6 642 21 0 1 182 218 0.0 6 210 8 183 582 0.0 6 565 15 0 0 2 184 295 0.0 6 285 8 1 1 185 306 0.0 6 288 18 186 949 0.0 6 930 19 187 657 0.0 6 643 14 188 708 0.0 6 684 23 1 189 627 0.0 6 612 15 190 1170 0.0 6 1135 34 1 191 933 0.0 6 910 22 0 0 1 192 788 0.0 6 769 18 0 0 1 193 462 0.0 6 446 14 1 1 194 676 0.0 6 653 22 1 195 710 0.0 6 677 29 1 2 0 0 1 196 1955 0.0 6 1913 40 1 1 197 686 0.0 6 664 20 1 0 1 198 1093 0.0 6 1062 30 1 199 1630 0.0 6 1590 38 1 0 1 200 581 0.0 6 566 14 0 1 201 813 0.0 6 798 14 1 202 436 0.0 6 419 16 0 0 1 203 311 0.0 6 295 15 1 204 341 0.0 6 338 3 205 140 0.0 6 136 4 206 115 0.0 6 111 2 1 0 0 1 207 84 0.0 6 80 3 0 1 208 188 0.0 6 180 7 0 0 1 209 122 0.0 6 118 4 210 176 0.0 6 166 8 2 211 140 0.0 6 135 4 1 212 235 0.0 6 232 3 213 91 0.0 6 89 2 214 154 0.0 6 149 4 0 0 1 215 218 0.0 6 212 6 216 297 0.0 6 292 5 217 230 0.0 6 227 3 218 175 0.0 6 169 6 219 212 0.0 6 205 6 1 220 127 0.0 6 126 1 221 180 0.0 6 176 3 0 0 1 222 211 0.0 6 205 6 223 370 0.0 6 361 9 224 138 0.0 6 136 2 225 255 0.0 6 251 4 226 141 0.0 6 137 3 0 1 227 109 0.0 6 105 3 1 228 152 0.0 6 149 3 229 182 0.0 6 180 2 230 88 0.0 6 86 2 231 72 0.0 6 71 1 232 185 0.0 6 181 4 233 183 0.0 6 176 6 0 0 1 234 37 0.0 6 36 1 235 32 0.0 6 31 1 236 20 0.0 6 20 237 16 0.0 6 14 2 238 6 0.0 6 6 239 27 0.0 6 26 1 240 47 0.0 6 45 2 241 48 0.0 6 45 3 242 107 0.0 6 104 3 243 77 0.0 6 74 2 0 1 244 53 0.0 6 51 2 245 24 0.0 6 22 2 246 25 0.0 6 25 247 6 0.0 6 6 248 25 0.0 6 24 1 249 9 0.0 6 9 250 6 0.0 6 6 251 2 0.0 6 2 252 3 0.0 6 2 1 253 1 0.0 6 1 254 10 0.0 6 10 255 1 0.0 6 1 277 1 0.0 6 1 279 17 0.0 6 16 1 280 15 0.0 6 14 1 281 1 0.0 6 1 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 18 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 1 0.0 6 1 73 1 0.0 6 1 74 1 0.0 6 1 75 1 0.0 6 1 78 2 0.0 6 2 79 1 0.0 6 1 80 1 0.0 6 1 83 2 0.0 6 2 85 1 0.0 6 1 87 1 0.0 6 1 88 1 0.0 6 1 89 1 0.0 6 1 97 1 0.0 6 1 102 1 0.0 6 1 110 1 0.0 6 1 129 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_13_198_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_13_425_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_13_198_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_13_425_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 7.02 s (152 µs/read; 0.40 M reads/minute). === Summary === Total read pairs processed: 46,244 Read 1 with adapter: 46,238 (100.0%) Read 2 with adapter: 46,222 (100.0%) == Read fate breakdown == Pairs that were too short: 2 (0.0%) Pairs discarded as untrimmed: 28 (0.1%) Pairs written (passing filters): 46,214 (99.9%) Total basepairs processed: 20,821,845 bp Read 1: 11,506,343 bp Read 2: 9,315,502 bp Total written (filtered): 2,940,117 bp (14.1%) Read 1: 1,521,469 bp Read 2: 1,418,648 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 45496 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 2 180.6 1 1 1 6 2 11.3 1 0 0 2 8 1 0.7 2 1 9 1 0.2 2 1 10 1 0.0 3 1 15 1 0.0 4 1 17 2 0.0 5 1 1 20 24 0.0 6 22 2 21 22 0.0 6 19 2 1 22 10 0.0 6 10 23 13 0.0 6 13 24 15 0.0 6 14 1 25 9 0.0 6 9 26 6 0.0 6 5 0 0 0 0 1 27 7 0.0 6 7 28 6 0.0 6 6 29 6 0.0 6 6 30 5 0.0 6 5 31 3 0.0 6 3 32 1 0.0 6 1 33 2 0.0 6 2 34 2 0.0 6 2 35 4 0.0 6 4 36 3 0.0 6 3 37 2 0.0 6 2 38 5 0.0 6 5 39 9 0.0 6 9 40 8 0.0 6 7 1 41 2 0.0 6 2 42 1 0.0 6 1 43 2 0.0 6 2 44 9 0.0 6 9 45 4 0.0 6 4 46 5 0.0 6 5 47 3 0.0 6 3 48 2 0.0 6 1 1 49 3 0.0 6 3 50 2 0.0 6 1 0 0 1 51 2 0.0 6 2 53 4 0.0 6 3 1 54 4 0.0 6 4 55 3 0.0 6 1 1 1 56 2 0.0 6 2 57 2 0.0 6 1 1 58 1 0.0 6 1 59 3 0.0 6 3 60 1 0.0 6 1 61 4 0.0 6 3 1 62 2 0.0 6 1 1 63 5 0.0 6 4 1 64 5 0.0 6 5 65 6 0.0 6 5 1 66 8 0.0 6 7 1 67 3 0.0 6 3 68 3 0.0 6 3 69 3 0.0 6 3 70 5 0.0 6 5 71 1 0.0 6 1 72 9 0.0 6 9 73 6 0.0 6 5 1 74 3 0.0 6 3 75 3 0.0 6 3 76 3 0.0 6 3 77 1 0.0 6 1 78 10 0.0 6 9 0 0 0 0 0 1 79 7 0.0 6 7 80 2 0.0 6 1 0 0 0 0 1 81 4 0.0 6 4 82 3 0.0 6 3 83 9 0.0 6 9 84 5 0.0 6 5 85 9 0.0 6 9 86 7 0.0 6 6 1 87 4 0.0 6 4 88 1 0.0 6 1 89 3 0.0 6 2 1 90 4 0.0 6 3 1 91 3 0.0 6 3 92 4 0.0 6 4 93 5 0.0 6 5 94 5 0.0 6 5 95 2 0.0 6 2 97 8 0.0 6 7 1 98 8 0.0 6 7 1 99 3 0.0 6 3 100 6 0.0 6 6 101 4 0.0 6 3 1 102 8 0.0 6 8 103 15 0.0 6 15 104 9 0.0 6 9 105 10 0.0 6 10 106 5 0.0 6 4 0 0 1 107 12 0.0 6 12 108 13 0.0 6 13 109 8 0.0 6 7 0 1 110 6 0.0 6 5 1 111 18 0.0 6 17 0 0 0 1 112 13 0.0 6 12 1 113 18 0.0 6 18 114 8 0.0 6 8 115 10 0.0 6 10 116 13 0.0 6 13 117 13 0.0 6 12 1 118 12 0.0 6 12 119 17 0.0 6 16 1 120 12 0.0 6 11 0 0 1 121 15 0.0 6 15 122 14 0.0 6 14 123 13 0.0 6 13 124 23 0.0 6 23 125 12 0.0 6 10 1 1 126 16 0.0 6 14 2 127 15 0.0 6 15 128 22 0.0 6 22 129 14 0.0 6 14 130 25 0.0 6 24 1 131 17 0.0 6 17 132 11 0.0 6 10 1 133 16 0.0 6 16 134 23 0.0 6 22 1 135 26 0.0 6 26 136 21 0.0 6 21 137 28 0.0 6 28 138 49 0.0 6 48 1 139 15 0.0 6 15 140 49 0.0 6 48 1 141 122 0.0 6 121 1 142 149 0.0 6 148 1 143 159 0.0 6 159 144 322 0.0 6 321 1 145 316 0.0 6 314 2 146 442 0.0 6 441 1 147 319 0.0 6 319 148 185 0.0 6 185 149 218 0.0 6 217 1 150 183 0.0 6 181 2 151 719 0.0 6 718 1 152 259 0.0 6 258 1 153 385 0.0 6 385 154 98 0.0 6 96 2 155 101 0.0 6 101 156 157 0.0 6 157 157 189 0.0 6 187 2 158 187 0.0 6 187 159 615 0.0 6 613 2 160 143 0.0 6 142 1 161 202 0.0 6 201 1 162 228 0.0 6 228 163 567 0.0 6 567 164 338 0.0 6 337 1 165 306 0.0 6 306 166 79 0.0 6 77 2 167 228 0.0 6 226 2 168 178 0.0 6 178 169 101 0.0 6 101 170 76 0.0 6 76 171 341 0.0 6 340 0 1 172 125 0.0 6 124 1 173 127 0.0 6 126 1 174 133 0.0 6 131 2 175 108 0.0 6 108 176 147 0.0 6 143 3 0 1 177 137 0.0 6 134 3 178 219 0.0 6 219 179 169 0.0 6 167 1 1 180 174 0.0 6 173 1 181 100 0.0 6 100 182 189 0.0 6 188 1 183 166 0.0 6 165 0 0 0 0 1 184 183 0.0 6 181 2 185 366 0.0 6 362 4 186 614 0.0 6 614 187 281 0.0 6 281 188 166 0.0 6 165 1 189 123 0.0 6 120 2 0 1 190 126 0.0 6 124 2 191 133 0.0 6 130 2 0 1 192 191 0.0 6 187 3 0 0 1 193 539 0.0 6 535 2 1 1 194 207 0.0 6 204 2 0 0 0 1 195 224 0.0 6 220 2 1 1 196 358 0.0 6 356 1 1 197 312 0.0 6 306 5 0 1 198 360 0.0 6 357 3 199 264 0.0 6 262 2 200 319 0.0 6 318 0 0 0 0 0 1 201 657 0.0 6 653 3 0 0 0 0 1 202 254 0.0 6 248 5 1 203 269 0.0 6 263 6 204 328 0.0 6 326 2 205 575 0.0 6 571 4 206 415 0.0 6 412 3 207 296 0.0 6 287 8 1 208 344 0.0 6 338 6 209 274 0.0 6 270 4 210 241 0.0 6 238 3 211 269 0.0 6 269 212 291 0.0 6 288 2 0 1 213 423 0.0 6 418 4 0 0 1 214 412 0.0 6 407 3 1 0 1 215 517 0.0 6 514 3 216 336 0.0 6 335 1 217 485 0.0 6 483 2 218 636 0.0 6 634 2 219 391 0.0 6 389 2 220 359 0.0 6 357 1 0 1 221 468 0.0 6 464 4 222 300 0.0 6 294 4 0 2 223 315 0.0 6 310 5 224 179 0.0 6 178 1 225 240 0.0 6 236 4 226 493 0.0 6 486 6 0 1 227 364 0.0 6 357 7 228 325 0.0 6 320 5 229 269 0.0 6 265 4 230 337 0.0 6 335 2 231 230 0.0 6 225 5 232 313 0.0 6 309 4 233 326 0.0 6 322 2 2 234 288 0.0 6 285 3 235 132 0.0 6 129 3 236 160 0.0 6 156 3 0 1 237 267 0.0 6 262 5 238 237 0.0 6 228 8 0 1 239 250 0.0 6 246 4 240 243 0.0 6 234 9 241 312 0.0 6 303 4 2 1 1 1 242 302 0.0 6 295 7 243 277 0.0 6 269 7 1 244 272 0.0 6 260 11 0 1 245 336 0.0 6 330 6 246 145 0.0 6 143 1 1 247 219 0.0 6 214 5 248 129 0.0 6 125 4 249 420 0.0 6 411 8 0 1 250 215 0.0 6 210 5 251 286 0.0 6 276 10 252 254 0.0 6 244 10 253 268 0.0 6 262 6 254 602 0.0 6 590 11 1 255 374 0.0 6 364 10 256 403 0.0 6 399 4 257 296 0.0 6 288 7 0 1 258 469 0.0 6 453 14 2 259 403 0.0 6 393 10 260 564 0.0 6 557 6 0 1 261 200 0.0 6 195 5 262 171 0.0 6 164 7 263 389 0.0 6 379 10 264 176 0.0 6 167 8 0 1 265 275 0.0 6 270 4 1 266 176 0.0 6 172 4 267 264 0.0 6 257 7 268 243 0.0 6 239 4 269 263 0.0 6 258 5 270 170 0.0 6 165 3 0 1 0 1 271 214 0.0 6 199 14 0 1 272 411 0.0 6 401 10 273 428 0.0 6 418 10 274 446 0.0 6 434 12 275 585 0.0 6 568 14 2 1 276 933 0.0 6 913 17 1 1 1 277 941 0.0 6 922 18 1 278 1547 0.0 6 1511 33 1 2 279 2028 0.0 6 1930 95 1 2 280 43 0.0 6 33 10 281 6 0.0 6 5 0 1 282 2 0.0 6 0 0 0 1 1 283 1 0.0 6 1 284 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 742 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 11 0.0 6 11 72 5 0.0 6 5 73 6 0.0 6 6 74 6 0.0 6 6 75 19 0.0 6 19 76 14 0.0 6 14 77 15 0.0 6 15 78 13 0.0 6 13 79 3 0.0 6 2 1 80 4 0.0 6 4 81 11 0.0 6 11 82 27 0.0 6 27 83 7 0.0 6 7 84 9 0.0 6 9 85 3 0.0 6 3 86 6 0.0 6 6 87 1 0.0 6 1 88 3 0.0 6 3 89 8 0.0 6 8 90 12 0.0 6 12 91 3 0.0 6 3 92 9 0.0 6 9 93 14 0.0 6 14 94 16 0.0 6 16 95 8 0.0 6 8 96 4 0.0 6 4 97 1 0.0 6 1 98 4 0.0 6 4 99 2 0.0 6 2 101 5 0.0 6 5 102 11 0.0 6 11 103 2 0.0 6 2 106 2 0.0 6 2 107 4 0.0 6 4 108 2 0.0 6 2 109 5 0.0 6 5 110 1 0.0 6 1 111 3 0.0 6 3 112 1 0.0 6 1 113 5 0.0 6 5 114 3 0.0 6 3 115 7 0.0 6 7 116 15 0.0 6 15 117 17 0.0 6 17 118 4 0.0 6 4 120 2 0.0 6 2 122 1 0.0 6 1 123 4 0.0 6 4 124 17 0.0 6 17 125 1 0.0 6 1 126 4 0.0 6 4 127 8 0.0 6 8 130 3 0.0 6 2 1 131 12 0.0 6 12 132 25 0.0 6 25 133 3 0.0 6 3 134 2 0.0 6 2 135 3 0.0 6 3 136 8 0.0 6 8 137 5 0.0 6 5 138 7 0.0 6 7 140 2 0.0 6 2 141 3 0.0 6 3 142 2 0.0 6 2 143 4 0.0 6 4 144 5 0.0 6 5 145 1 0.0 6 1 146 4 0.0 6 4 147 4 0.0 6 4 148 15 0.0 6 15 149 24 0.0 6 24 150 7 0.0 6 7 151 25 0.0 6 25 152 29 0.0 6 29 153 7 0.0 6 7 154 11 0.0 6 11 155 3 0.0 6 3 157 2 0.0 6 2 158 5 0.0 6 5 159 5 0.0 6 5 160 2 0.0 6 2 161 2 0.0 6 2 162 3 0.0 6 3 163 1 0.0 6 1 164 5 0.0 6 5 165 8 0.0 6 8 168 3 0.0 6 3 169 9 0.0 6 9 170 2 0.0 6 2 171 1 0.0 6 1 172 2 0.0 6 2 173 2 0.0 6 2 174 2 0.0 6 2 176 2 0.0 6 2 177 4 0.0 6 4 178 15 0.0 6 15 179 12 0.0 6 12 182 2 0.0 6 2 189 1 0.0 6 1 191 4 0.0 6 4 192 1 0.0 6 1 193 3 0.0 6 3 194 5 0.0 6 5 196 1 0.0 6 1 199 1 0.0 6 1 201 1 0.0 6 1 203 5 0.0 6 5 204 4 0.0 6 4 205 1 0.0 6 1 207 1 0.0 6 1 208 6 0.0 6 6 210 1 0.0 6 1 213 1 0.0 6 1 214 3 0.0 6 3 217 2 0.0 6 2 222 3 0.0 6 3 223 4 0.0 6 4 224 1 0.0 6 1 226 1 0.0 6 1 232 3 0.0 6 3 233 1 0.0 6 1 240 1 0.0 6 1 241 1 0.0 6 1 242 1 0.0 6 1 246 1 0.0 6 1 248 1 0.0 6 1 249 1 0.0 6 1 250 2 0.0 6 2 253 1 0.0 6 1 255 1 0.0 6 1 259 1 0.0 6 1 260 1 0.0 6 1 262 1 0.0 6 1 263 1 0.0 6 1 269 1 0.0 6 1 283 1 0.0 6 1 288 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 45434 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 11.3 1 1 7 1 2.8 2 0 1 9 1 0.2 2 1 13 3 0.0 3 3 14 3 0.0 4 2 1 15 2 0.0 4 2 16 6 0.0 4 6 17 5 0.0 5 4 1 18 7 0.0 5 5 2 19 14 0.0 5 12 2 20 18 0.0 6 16 2 21 20 0.0 6 20 22 21 0.0 6 21 23 14 0.0 6 14 24 7 0.0 6 6 1 25 6 0.0 6 6 26 4 0.0 6 4 27 4 0.0 6 4 28 8 0.0 6 8 29 4 0.0 6 4 30 6 0.0 6 6 31 6 0.0 6 6 32 9 0.0 6 9 33 5 0.0 6 4 1 34 3 0.0 6 2 1 35 7 0.0 6 7 36 8 0.0 6 7 1 37 5 0.0 6 4 1 38 5 0.0 6 5 39 4 0.0 6 4 40 8 0.0 6 8 41 3 0.0 6 3 42 2 0.0 6 2 43 5 0.0 6 5 44 18 0.0 6 18 45 7 0.0 6 6 1 46 10 0.0 6 9 0 1 47 6 0.0 6 4 2 48 3 0.0 6 3 49 13 0.0 6 13 50 11 0.0 6 10 1 51 5 0.0 6 4 0 0 1 52 7 0.0 6 7 53 8 0.0 6 8 54 12 0.0 6 11 1 55 14 0.0 6 13 1 56 7 0.0 6 6 1 57 7 0.0 6 7 58 13 0.0 6 13 59 12 0.0 6 10 2 60 9 0.0 6 9 61 6 0.0 6 6 62 17 0.0 6 17 63 14 0.0 6 14 64 17 0.0 6 17 65 15 0.0 6 14 1 66 13 0.0 6 11 2 67 9 0.0 6 9 68 16 0.0 6 14 2 69 90 0.0 6 85 5 70 28 0.0 6 28 71 37 0.0 6 37 72 23 0.0 6 22 1 73 26 0.0 6 25 0 0 1 74 14 0.0 6 14 75 6 0.0 6 6 76 21 0.0 6 20 0 0 0 1 77 19 0.0 6 19 78 25 0.0 6 25 79 19 0.0 6 18 1 80 18 0.0 6 18 81 15 0.0 6 13 1 0 0 0 1 82 20 0.0 6 17 3 83 25 0.0 6 24 1 84 18 0.0 6 18 85 39 0.0 6 37 2 86 15 0.0 6 14 1 87 17 0.0 6 16 1 88 21 0.0 6 21 89 17 0.0 6 17 90 23 0.0 6 22 1 91 19 0.0 6 17 2 92 31 0.0 6 29 1 0 1 93 23 0.0 6 22 1 94 46 0.0 6 41 4 0 1 95 21 0.0 6 21 96 28 0.0 6 26 1 1 97 25 0.0 6 22 2 1 98 34 0.0 6 33 1 99 31 0.0 6 29 1 0 0 1 100 32 0.0 6 32 101 29 0.0 6 29 102 39 0.0 6 36 2 0 0 0 1 103 48 0.0 6 46 2 104 54 0.0 6 53 1 105 46 0.0 6 44 2 106 63 0.0 6 63 107 52 0.0 6 51 1 108 48 0.0 6 47 1 109 52 0.0 6 51 1 110 58 0.0 6 58 111 43 0.0 6 40 3 112 53 0.0 6 49 3 1 113 50 0.0 6 49 1 114 61 0.0 6 59 2 115 59 0.0 6 56 3 116 64 0.0 6 61 3 117 60 0.0 6 58 1 0 1 118 67 0.0 6 66 1 119 90 0.0 6 88 2 120 96 0.0 6 93 0 1 0 2 121 63 0.0 6 60 2 1 122 75 0.0 6 74 1 123 80 0.0 6 78 2 124 94 0.0 6 92 2 125 72 0.0 6 71 1 126 102 0.0 6 100 1 0 0 0 1 127 128 0.0 6 128 128 101 0.0 6 97 3 1 129 90 0.0 6 90 130 124 0.0 6 123 1 131 129 0.0 6 123 5 1 132 160 0.0 6 159 1 133 180 0.0 6 175 5 134 197 0.0 6 192 5 135 239 0.0 6 233 5 1 136 186 0.0 6 183 3 137 215 0.0 6 212 3 138 193 0.0 6 186 6 1 139 192 0.0 6 190 2 140 326 0.0 6 321 4 1 141 346 0.0 6 340 6 142 356 0.0 6 353 3 143 509 0.0 6 503 6 144 575 0.0 6 570 5 145 526 0.0 6 525 1 146 502 0.0 6 498 4 147 396 0.0 6 391 4 0 0 0 0 1 148 371 0.0 6 369 2 149 486 0.0 6 479 5 1 0 0 0 1 150 934 0.0 6 927 6 0 0 1 151 497 0.0 6 484 12 0 0 1 152 821 0.0 6 813 8 153 471 0.0 6 464 7 154 552 0.0 6 538 13 1 155 608 0.0 6 600 8 156 486 0.0 6 481 5 157 645 0.0 6 642 2 1 158 798 0.0 6 783 14 0 0 1 159 487 0.0 6 485 2 160 695 0.0 6 684 10 1 161 629 0.0 6 624 4 0 0 0 0 1 162 687 0.0 6 684 2 0 0 0 1 163 526 0.0 6 519 5 2 164 414 0.0 6 408 6 165 496 0.0 6 485 10 0 0 0 0 1 166 646 0.0 6 636 9 1 167 334 0.0 6 331 3 168 648 0.0 6 639 9 169 410 0.0 6 407 3 170 466 0.0 6 459 7 171 545 0.0 6 537 7 0 0 0 1 172 321 0.0 6 317 4 173 385 0.0 6 382 3 174 429 0.0 6 423 6 175 609 0.0 6 602 4 3 176 467 0.0 6 462 5 177 554 0.0 6 547 6 1 178 503 0.0 6 497 6 179 506 0.0 6 499 6 1 180 494 0.0 6 486 8 181 505 0.0 6 493 9 1 0 1 0 1 182 296 0.0 6 291 4 0 0 1 183 502 0.0 6 493 9 184 292 0.0 6 285 7 185 420 0.0 6 413 7 186 873 0.0 6 853 19 1 187 821 0.0 6 801 19 0 0 1 188 722 0.0 6 706 16 189 577 0.0 6 561 14 2 190 766 0.0 6 747 16 2 0 1 191 623 0.0 6 610 12 1 192 529 0.0 6 515 13 0 1 193 560 0.0 6 547 12 1 194 448 0.0 6 436 12 195 688 0.0 6 666 22 196 835 0.0 6 818 16 1 197 250 0.0 6 242 7 1 198 871 0.0 6 842 29 199 1136 0.0 6 1103 32 0 1 200 394 0.0 6 384 10 201 391 0.0 6 384 7 202 294 0.0 6 288 5 1 203 244 0.0 6 233 9 1 0 1 204 329 0.0 6 325 4 205 230 0.0 6 222 8 206 141 0.0 6 137 4 207 142 0.0 6 142 208 235 0.0 6 230 4 0 1 209 189 0.0 6 185 4 210 125 0.0 6 121 3 1 211 193 0.0 6 190 3 212 156 0.0 6 150 6 213 106 0.0 6 101 4 0 0 1 214 138 0.0 6 132 6 215 103 0.0 6 100 3 216 313 0.0 6 306 7 217 191 0.0 6 183 7 0 0 1 218 149 0.0 6 145 2 0 1 1 219 97 0.0 6 92 5 220 105 0.0 6 99 6 221 95 0.0 6 92 3 222 212 0.0 6 205 6 1 223 414 0.0 6 406 8 224 137 0.0 6 132 5 225 172 0.0 6 164 7 1 226 117 0.0 6 114 3 227 231 0.0 6 230 1 228 62 0.0 6 62 229 181 0.0 6 175 6 230 53 0.0 6 49 3 0 1 231 109 0.0 6 108 1 232 88 0.0 6 87 1 233 137 0.0 6 136 1 234 109 0.0 6 107 2 235 37 0.0 6 37 236 21 0.0 6 21 237 15 0.0 6 15 238 20 0.0 6 18 2 239 6 0.0 6 6 240 33 0.0 6 32 1 241 30 0.0 6 29 1 242 53 0.0 6 52 1 243 103 0.0 6 101 2 244 46 0.0 6 44 1 1 245 26 0.0 6 25 1 246 40 0.0 6 39 1 247 17 0.0 6 17 248 36 0.0 6 33 2 0 0 1 249 45 0.0 6 45 250 19 0.0 6 18 1 251 20 0.0 6 20 252 12 0.0 6 12 253 21 0.0 6 20 1 254 26 0.0 6 26 255 19 0.0 6 19 256 2 0.0 6 2 257 1 0.0 6 1 258 2 0.0 6 2 279 5 0.0 6 4 1 280 35 0.0 6 35 281 120 0.0 6 117 1 0 0 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 788 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 18 1 0.0 5 0 0 0 0 0 1 70 2 0.0 6 2 71 8 0.0 6 8 72 5 0.0 6 5 73 10 0.0 6 10 74 13 0.0 6 13 75 22 0.0 6 22 76 11 0.0 6 11 77 3 0.0 6 3 78 9 0.0 6 8 0 0 1 79 4 0.0 6 4 80 71 0.0 6 71 81 7 0.0 6 7 82 17 0.0 6 17 84 3 0.0 6 2 0 0 0 1 85 6 0.0 6 6 86 8 0.0 6 8 87 10 0.0 6 10 88 21 0.0 6 21 89 5 0.0 6 5 90 10 0.0 6 10 91 3 0.0 6 3 92 32 0.0 6 32 93 12 0.0 6 12 94 4 0.0 6 4 95 1 0.0 6 1 96 7 0.0 6 7 97 6 0.0 6 6 98 4 0.0 6 4 99 3 0.0 6 3 100 16 0.0 6 15 0 0 0 1 101 2 0.0 6 2 102 2 0.0 6 2 103 3 0.0 6 3 104 2 0.0 6 2 105 4 0.0 6 4 106 4 0.0 6 4 107 8 0.0 6 7 0 0 0 1 108 4 0.0 6 4 109 2 0.0 6 2 110 3 0.0 6 3 111 6 0.0 6 6 112 1 0.0 6 1 113 5 0.0 6 5 114 6 0.0 6 6 115 20 0.0 6 20 116 4 0.0 6 3 0 0 0 0 0 1 118 1 0.0 6 1 121 1 0.0 6 1 122 13 0.0 6 13 123 2 0.0 6 2 125 10 0.0 6 10 128 1 0.0 6 1 129 10 0.0 6 10 130 18 0.0 6 18 131 3 0.0 6 3 132 2 0.0 6 2 133 3 0.0 6 3 134 12 0.0 6 12 135 5 0.0 6 5 136 6 0.0 6 6 138 4 0.0 6 2 2 139 31 0.0 6 31 140 3 0.0 6 3 141 3 0.0 6 3 142 13 0.0 6 13 143 3 0.0 6 3 144 2 0.0 6 2 145 3 0.0 6 3 146 12 0.0 6 12 147 31 0.0 6 30 0 0 0 1 148 4 0.0 6 4 149 20 0.0 6 20 150 35 0.0 6 35 151 2 0.0 6 2 152 6 0.0 6 6 155 1 0.0 6 1 156 6 0.0 6 6 157 5 0.0 6 5 158 3 0.0 6 3 159 2 0.0 6 2 160 6 0.0 6 6 161 1 0.0 6 1 162 4 0.0 6 4 163 17 0.0 6 17 164 1 0.0 6 1 165 1 0.0 6 1 166 4 0.0 6 4 167 4 0.0 6 4 168 6 0.0 6 5 1 169 1 0.0 6 1 170 1 0.0 6 1 175 6 0.0 6 6 176 10 0.0 6 10 177 7 0.0 6 7 186 2 0.0 6 2 187 2 0.0 6 2 188 2 0.0 6 2 190 2 0.0 6 2 191 2 0.0 6 2 192 2 0.0 6 2 194 6 0.0 6 6 197 2 0.0 6 2 200 3 0.0 6 3 201 2 0.0 6 2 202 1 0.0 6 1 206 3 0.0 6 3 211 1 0.0 6 1 251 1 0.0 6 1 252 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_07_199_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_07_426_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_07_199_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_07_426_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 8.54 s (80 µs/read; 0.75 M reads/minute). === Summary === Total read pairs processed: 106,675 Read 1 with adapter: 106,669 (100.0%) Read 2 with adapter: 106,616 (99.9%) == Read fate breakdown == Pairs that were too short: 35 (0.0%) Pairs discarded as untrimmed: 65 (0.1%) Pairs written (passing filters): 106,575 (99.9%) Total basepairs processed: 48,935,711 bp Read 1: 27,402,319 bp Read 2: 21,533,392 bp Total written (filtered): 6,738,111 bp (13.8%) Read 1: 3,289,967 bp Read 2: 3,448,144 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 104991 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 1666.8 0 2 2 4 3 416.7 1 0 3 5 2 104.2 1 2 8 3 1.6 2 2 1 9 2 0.4 2 2 10 2 0.1 3 1 1 16 2 0.0 4 2 17 1 0.0 5 1 18 2 0.0 5 2 19 2 0.0 5 1 1 20 119 0.0 6 118 1 21 51 0.0 6 49 2 22 47 0.0 6 47 23 34 0.0 6 33 1 24 24 0.0 6 22 2 25 27 0.0 6 27 26 19 0.0 6 17 2 27 8 0.0 6 8 28 6 0.0 6 6 29 8 0.0 6 8 30 5 0.0 6 5 31 10 0.0 6 10 32 7 0.0 6 6 1 33 4 0.0 6 4 34 15 0.0 6 15 35 8 0.0 6 7 0 1 36 6 0.0 6 6 37 7 0.0 6 7 38 8 0.0 6 8 39 14 0.0 6 13 0 0 0 0 0 1 40 2 0.0 6 2 41 8 0.0 6 8 42 5 0.0 6 5 43 7 0.0 6 7 44 11 0.0 6 11 45 7 0.0 6 7 46 4 0.0 6 4 47 4 0.0 6 4 48 5 0.0 6 5 49 4 0.0 6 4 50 6 0.0 6 6 51 7 0.0 6 7 52 10 0.0 6 9 1 53 6 0.0 6 6 54 5 0.0 6 4 1 55 8 0.0 6 6 2 56 8 0.0 6 8 57 5 0.0 6 5 58 5 0.0 6 5 59 5 0.0 6 5 60 5 0.0 6 5 61 4 0.0 6 4 62 5 0.0 6 5 63 10 0.0 6 9 0 0 0 0 1 64 6 0.0 6 6 65 6 0.0 6 6 66 5 0.0 6 5 67 10 0.0 6 10 68 9 0.0 6 8 1 69 10 0.0 6 7 3 70 5 0.0 6 5 71 5 0.0 6 5 72 9 0.0 6 9 73 7 0.0 6 5 2 74 5 0.0 6 5 75 9 0.0 6 9 76 5 0.0 6 4 1 77 13 0.0 6 12 1 78 12 0.0 6 12 79 7 0.0 6 7 80 11 0.0 6 11 81 7 0.0 6 7 82 6 0.0 6 6 83 8 0.0 6 7 1 84 9 0.0 6 9 85 8 0.0 6 8 86 16 0.0 6 16 87 8 0.0 6 8 88 14 0.0 6 14 89 9 0.0 6 9 90 7 0.0 6 7 91 10 0.0 6 10 92 7 0.0 6 7 93 7 0.0 6 6 1 94 9 0.0 6 9 95 5 0.0 6 4 1 96 7 0.0 6 7 97 10 0.0 6 10 98 6 0.0 6 6 99 10 0.0 6 10 100 9 0.0 6 9 101 17 0.0 6 17 102 21 0.0 6 19 2 103 19 0.0 6 19 104 15 0.0 6 14 1 105 13 0.0 6 12 1 106 17 0.0 6 17 107 17 0.0 6 17 108 22 0.0 6 22 109 20 0.0 6 20 110 10 0.0 6 10 111 14 0.0 6 14 112 16 0.0 6 15 1 113 14 0.0 6 14 114 22 0.0 6 20 2 115 25 0.0 6 24 1 116 23 0.0 6 23 117 19 0.0 6 19 118 18 0.0 6 18 119 17 0.0 6 17 120 12 0.0 6 10 1 0 0 0 1 121 19 0.0 6 16 3 122 23 0.0 6 22 1 123 15 0.0 6 14 1 124 27 0.0 6 26 1 125 31 0.0 6 31 126 29 0.0 6 28 1 127 40 0.0 6 40 128 27 0.0 6 26 1 129 33 0.0 6 31 2 130 35 0.0 6 35 131 37 0.0 6 35 1 0 0 0 0 1 132 35 0.0 6 34 1 133 47 0.0 6 45 2 134 39 0.0 6 37 2 135 35 0.0 6 33 2 136 52 0.0 6 51 1 137 63 0.0 6 62 1 138 41 0.0 6 40 1 139 39 0.0 6 37 2 140 49 0.0 6 48 1 141 66 0.0 6 64 2 142 78 0.0 6 77 1 143 118 0.0 6 118 144 242 0.0 6 240 2 145 422 0.0 6 420 2 146 268 0.0 6 267 1 147 349 0.0 6 349 148 340 0.0 6 340 149 322 0.0 6 321 1 150 325 0.0 6 323 2 151 603 0.0 6 598 5 152 323 0.0 6 322 1 153 301 0.0 6 301 154 360 0.0 6 357 3 155 248 0.0 6 245 3 156 243 0.0 6 243 157 299 0.0 6 296 3 158 267 0.0 6 267 159 232 0.0 6 232 160 276 0.0 6 273 3 161 276 0.0 6 273 3 162 309 0.0 6 306 3 163 402 0.0 6 402 164 343 0.0 6 343 165 370 0.0 6 369 1 166 458 0.0 6 454 2 2 167 258 0.0 6 256 2 168 240 0.0 6 238 2 169 293 0.0 6 290 3 170 268 0.0 6 265 3 171 299 0.0 6 295 3 0 1 172 312 0.0 6 307 4 1 173 436 0.0 6 436 174 319 0.0 6 318 1 175 392 0.0 6 389 2 0 0 0 1 176 303 0.0 6 301 2 177 390 0.0 6 389 1 178 454 0.0 6 452 2 179 462 0.0 6 462 180 495 0.0 6 491 4 181 1487 0.0 6 1485 2 182 631 0.0 6 627 3 0 0 0 0 1 183 324 0.0 6 324 184 481 0.0 6 481 185 510 0.0 6 510 186 505 0.0 6 502 2 0 0 0 1 187 445 0.0 6 439 5 0 1 188 608 0.0 6 604 4 189 436 0.0 6 432 4 190 584 0.0 6 581 3 191 532 0.0 6 529 3 192 553 0.0 6 551 1 0 0 1 193 491 0.0 6 485 5 0 1 194 560 0.0 6 559 1 195 589 0.0 6 588 1 196 838 0.0 6 829 9 197 778 0.0 6 775 3 198 794 0.0 6 786 8 199 1109 0.0 6 1105 4 200 933 0.0 6 923 10 201 763 0.0 6 757 6 202 694 0.0 6 690 4 203 690 0.0 6 683 7 204 750 0.0 6 746 4 205 1098 0.0 6 1092 6 206 635 0.0 6 624 9 0 2 207 686 0.0 6 679 5 1 0 0 1 208 870 0.0 6 864 6 209 718 0.0 6 713 4 0 0 0 0 1 210 817 0.0 6 808 8 0 0 0 0 1 211 715 0.0 6 710 4 1 212 717 0.0 6 707 10 213 856 0.0 6 852 4 214 865 0.0 6 854 11 215 872 0.0 6 865 7 216 965 0.0 6 955 10 217 1152 0.0 6 1147 4 1 218 850 0.0 6 840 9 1 219 931 0.0 6 924 6 1 220 983 0.0 6 977 6 221 894 0.0 6 886 7 1 222 831 0.0 6 820 11 223 854 0.0 6 846 8 224 856 0.0 6 847 8 1 225 881 0.0 6 873 8 226 964 0.0 6 953 10 0 1 227 945 0.0 6 936 8 1 228 961 0.0 6 952 8 0 0 1 229 780 0.0 6 775 3 0 1 0 0 1 230 763 0.0 6 755 5 3 231 765 0.0 6 759 6 232 745 0.0 6 737 8 233 829 0.0 6 825 3 1 234 813 0.0 6 807 6 235 783 0.0 6 773 8 2 236 869 0.0 6 855 12 2 237 791 0.0 6 782 8 0 0 0 1 238 774 0.0 6 764 10 239 691 0.0 6 682 8 0 1 240 622 0.0 6 616 6 241 801 0.0 6 790 11 242 634 0.0 6 621 13 243 852 0.0 6 834 18 244 699 0.0 6 691 8 245 765 0.0 6 747 18 246 534 0.0 6 522 12 247 1105 0.0 6 1085 20 248 502 0.0 6 484 16 1 0 0 0 1 249 619 0.0 6 605 11 1 1 1 250 564 0.0 6 555 9 251 526 0.0 6 516 10 252 502 0.0 6 493 9 253 654 0.0 6 635 19 254 634 0.0 6 618 16 255 622 0.0 6 611 11 256 842 0.0 6 830 12 257 456 0.0 6 443 13 258 577 0.0 6 565 11 1 259 667 0.0 6 649 18 260 885 0.0 6 859 25 0 1 261 563 0.0 6 552 11 262 435 0.0 6 421 14 263 496 0.0 6 481 14 0 0 1 264 555 0.0 6 548 7 265 530 0.0 6 518 11 0 1 266 540 0.0 6 526 13 1 267 444 0.0 6 429 15 268 873 0.0 6 853 19 1 269 382 0.0 6 369 13 270 467 0.0 6 454 11 2 271 520 0.0 6 509 10 0 1 272 682 0.0 6 658 24 273 751 0.0 6 727 24 274 710 0.0 6 689 21 275 1006 0.0 6 976 29 1 276 1187 0.0 6 1149 37 1 277 1709 0.0 6 1662 46 1 278 2340 0.0 6 2256 83 1 279 3965 0.0 6 3865 96 3 1 280 6299 0.0 6 6143 151 5 281 6095 0.0 6 5962 131 2 282 36 0.0 6 30 6 283 4 0.0 6 4 284 3 0.0 6 1 0 0 2 286 2 0.0 6 2 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1678 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 2 0.0 6 1 1 61 81 0.0 6 77 3 1 69 2 0.0 6 2 70 5 0.0 6 5 71 8 0.0 6 7 1 72 28 0.0 6 28 73 4 0.0 6 4 74 6 0.0 6 6 75 10 0.0 6 10 76 11 0.0 6 11 77 10 0.0 6 10 78 13 0.0 6 13 79 11 0.0 6 11 80 11 0.0 6 11 81 7 0.0 6 7 82 6 0.0 6 6 83 7 0.0 6 7 84 6 0.0 6 6 85 8 0.0 6 8 86 3 0.0 6 3 87 8 0.0 6 8 88 9 0.0 6 9 89 4 0.0 6 4 90 15 0.0 6 15 91 4 0.0 6 4 92 11 0.0 6 11 93 10 0.0 6 10 94 2 0.0 6 2 95 11 0.0 6 11 96 4 0.0 6 4 97 9 0.0 6 9 98 6 0.0 6 6 99 8 0.0 6 8 100 11 0.0 6 11 101 8 0.0 6 8 102 8 0.0 6 8 103 4 0.0 6 4 104 6 0.0 6 6 105 13 0.0 6 13 106 6 0.0 6 6 107 15 0.0 6 15 108 58 0.0 6 57 1 109 14 0.0 6 14 110 10 0.0 6 10 111 16 0.0 6 16 112 12 0.0 6 12 113 4 0.0 6 4 114 11 0.0 6 11 115 7 0.0 6 7 116 11 0.0 6 11 117 19 0.0 6 19 118 10 0.0 6 10 119 8 0.0 6 8 120 10 0.0 6 10 121 11 0.0 6 11 122 13 0.0 6 13 123 17 0.0 6 17 124 13 0.0 6 13 125 15 0.0 6 15 126 14 0.0 6 14 127 6 0.0 6 6 128 7 0.0 6 7 129 10 0.0 6 10 130 4 0.0 6 4 131 14 0.0 6 13 0 0 0 0 0 1 132 22 0.0 6 22 133 12 0.0 6 12 134 9 0.0 6 9 135 8 0.0 6 8 136 1 0.0 6 1 137 7 0.0 6 7 138 8 0.0 6 8 139 7 0.0 6 7 140 8 0.0 6 8 141 11 0.0 6 11 142 5 0.0 6 5 143 11 0.0 6 11 144 12 0.0 6 12 145 13 0.0 6 13 146 18 0.0 6 18 147 16 0.0 6 16 148 11 0.0 6 11 149 14 0.0 6 14 150 14 0.0 6 13 0 0 1 151 5 0.0 6 5 152 6 0.0 6 6 153 7 0.0 6 7 154 4 0.0 6 4 155 7 0.0 6 7 156 23 0.0 6 23 157 13 0.0 6 13 158 9 0.0 6 9 159 17 0.0 6 17 160 14 0.0 6 14 161 13 0.0 6 13 162 22 0.0 6 22 163 19 0.0 6 19 164 21 0.0 6 21 165 36 0.0 6 36 166 6 0.0 6 6 167 14 0.0 6 14 168 13 0.0 6 13 169 5 0.0 6 5 170 6 0.0 6 6 171 13 0.0 6 13 172 12 0.0 6 12 173 8 0.0 6 8 174 12 0.0 6 12 175 9 0.0 6 9 176 9 0.0 6 9 177 12 0.0 6 12 178 8 0.0 6 8 179 6 0.0 6 6 180 7 0.0 6 7 181 4 0.0 6 4 182 5 0.0 6 5 183 12 0.0 6 12 184 7 0.0 6 7 185 11 0.0 6 11 186 11 0.0 6 11 187 8 0.0 6 8 188 20 0.0 6 20 189 6 0.0 6 6 190 6 0.0 6 6 191 8 0.0 6 8 192 7 0.0 6 7 193 22 0.0 6 22 194 13 0.0 6 13 195 6 0.0 6 6 196 5 0.0 6 5 197 4 0.0 6 4 198 11 0.0 6 11 199 8 0.0 6 8 200 1 0.0 6 1 201 6 0.0 6 6 202 7 0.0 6 7 203 2 0.0 6 2 204 7 0.0 6 7 205 1 0.0 6 1 207 3 0.0 6 3 208 8 0.0 6 8 209 5 0.0 6 5 210 9 0.0 6 9 211 6 0.0 6 6 212 4 0.0 6 4 213 5 0.0 6 5 215 2 0.0 6 2 216 8 0.0 6 8 217 5 0.0 6 5 218 4 0.0 6 4 219 2 0.0 6 2 220 5 0.0 6 5 221 3 0.0 6 3 222 1 0.0 6 1 223 6 0.0 6 6 224 4 0.0 6 4 225 3 0.0 6 3 227 4 0.0 6 4 228 4 0.0 6 4 229 10 0.0 6 10 230 5 0.0 6 5 231 3 0.0 6 3 233 3 0.0 6 3 234 2 0.0 6 2 235 1 0.0 6 1 236 8 0.0 6 8 240 4 0.0 6 4 241 1 0.0 6 1 243 4 0.0 6 4 244 1 0.0 6 1 245 2 0.0 6 2 246 2 0.0 6 2 247 6 0.0 6 6 248 1 0.0 6 1 249 2 0.0 6 2 250 3 0.0 6 3 251 2 0.0 6 2 252 2 0.0 6 2 253 1 0.0 6 1 254 2 0.0 6 2 256 2 0.0 6 2 257 3 0.0 6 3 258 3 0.0 6 3 260 3 0.0 6 3 262 1 0.0 6 1 263 2 0.0 6 2 265 1 0.0 6 1 266 1 0.0 6 1 268 2 0.0 6 2 269 1 0.0 6 1 271 1 0.0 6 1 274 2 0.0 6 2 275 2 0.0 6 2 276 2 0.0 6 2 277 1 0.0 6 1 278 2 0.0 6 2 279 1 0.0 6 1 284 1 0.0 6 1 290 1 0.0 6 1 291 3 0.0 6 3 292 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 105087 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1666.8 0 0 1 4 1 416.7 1 1 7 1 6.5 2 0 1 10 4 0.1 3 4 11 10 0.0 3 8 1 0 0 1 12 2 0.0 3 2 13 15 0.0 3 12 3 14 8 0.0 4 6 2 15 2 0.0 4 1 1 16 17 0.0 4 15 2 17 33 0.0 5 30 3 18 9 0.0 5 9 19 14 0.0 5 14 20 120 0.0 6 118 2 21 90 0.0 6 85 5 22 37 0.0 6 33 4 23 40 0.0 6 39 1 24 36 0.0 6 35 1 25 34 0.0 6 30 3 0 1 26 23 0.0 6 22 1 27 25 0.0 6 24 1 28 28 0.0 6 26 1 0 1 29 19 0.0 6 19 30 25 0.0 6 22 2 0 0 1 31 26 0.0 6 26 32 17 0.0 6 17 33 19 0.0 6 18 1 34 22 0.0 6 20 0 1 1 35 20 0.0 6 20 36 18 0.0 6 16 2 37 19 0.0 6 19 38 21 0.0 6 17 4 39 17 0.0 6 17 40 30 0.0 6 27 3 41 13 0.0 6 11 2 42 19 0.0 6 16 2 1 43 14 0.0 6 14 44 21 0.0 6 20 1 45 17 0.0 6 13 3 1 46 30 0.0 6 27 3 47 23 0.0 6 22 1 48 20 0.0 6 17 2 1 49 23 0.0 6 21 2 50 20 0.0 6 18 1 0 0 0 1 51 18 0.0 6 16 0 1 1 52 26 0.0 6 22 4 53 23 0.0 6 21 2 54 33 0.0 6 25 7 1 55 25 0.0 6 24 1 56 21 0.0 6 18 3 57 39 0.0 6 36 2 1 58 30 0.0 6 28 0 2 59 27 0.0 6 25 2 60 35 0.0 6 29 6 61 27 0.0 6 25 2 62 23 0.0 6 21 1 0 0 1 63 38 0.0 6 30 8 64 26 0.0 6 24 2 65 40 0.0 6 37 3 66 50 0.0 6 50 67 223 0.0 6 213 7 0 0 2 1 68 61 0.0 6 55 5 0 1 69 83 0.0 6 79 4 70 42 0.0 6 40 1 1 71 60 0.0 6 54 6 72 40 0.0 6 36 3 1 73 33 0.0 6 29 4 74 34 0.0 6 33 1 75 48 0.0 6 43 4 0 1 76 50 0.0 6 47 3 77 29 0.0 6 28 1 78 34 0.0 6 30 3 0 0 0 0 1 79 62 0.0 6 57 5 80 64 0.0 6 63 1 81 65 0.0 6 59 6 82 73 0.0 6 70 2 0 1 83 107 0.0 6 102 4 1 84 55 0.0 6 50 5 85 46 0.0 6 43 3 86 55 0.0 6 53 1 0 1 87 69 0.0 6 64 5 88 53 0.0 6 50 3 89 72 0.0 6 66 5 1 90 70 0.0 6 67 2 0 0 1 91 75 0.0 6 71 4 92 75 0.0 6 75 93 111 0.0 6 104 7 94 94 0.0 6 88 6 95 86 0.0 6 84 2 96 91 0.0 6 86 5 97 87 0.0 6 83 4 98 87 0.0 6 78 9 99 71 0.0 6 68 3 100 106 0.0 6 97 8 1 101 95 0.0 6 90 5 102 116 0.0 6 110 6 103 144 0.0 6 135 6 1 0 1 0 1 104 191 0.0 6 183 5 1 1 0 0 1 105 128 0.0 6 123 4 0 0 1 106 107 0.0 6 103 3 0 0 0 0 1 107 133 0.0 6 131 1 1 108 126 0.0 6 117 9 109 147 0.0 6 143 3 1 110 122 0.0 6 112 8 1 0 0 0 1 111 148 0.0 6 139 8 0 0 1 112 152 0.0 6 146 6 113 128 0.0 6 122 6 114 168 0.0 6 160 7 1 115 156 0.0 6 153 3 116 156 0.0 6 146 7 1 0 0 0 2 117 166 0.0 6 163 3 118 188 0.0 6 179 7 1 1 119 182 0.0 6 175 5 0 0 1 1 120 206 0.0 6 198 8 121 194 0.0 6 190 3 0 0 1 122 229 0.0 6 221 8 123 234 0.0 6 230 1 2 0 0 0 1 124 247 0.0 6 237 9 0 0 1 125 283 0.0 6 273 9 1 126 315 0.0 6 307 8 127 301 0.0 6 293 7 0 1 128 325 0.0 6 320 5 129 398 0.0 6 390 7 0 0 0 1 130 366 0.0 6 355 10 1 131 381 0.0 6 369 12 132 440 0.0 6 428 10 1 0 1 133 480 0.0 6 463 15 1 0 1 134 558 0.0 6 547 10 1 135 576 0.0 6 562 13 0 1 136 541 0.0 6 528 10 1 1 0 1 137 552 0.0 6 540 10 0 0 2 138 574 0.0 6 562 11 0 0 1 139 662 0.0 6 644 17 0 0 1 140 913 0.0 6 891 19 2 0 1 141 871 0.0 6 854 13 3 0 0 1 142 969 0.0 6 952 15 2 143 921 0.0 6 904 15 0 0 1 0 1 144 1044 0.0 6 1026 16 0 0 1 0 1 145 1024 0.0 6 1004 20 146 1204 0.0 6 1185 15 1 0 1 0 2 147 960 0.0 6 946 13 0 0 0 1 148 1141 0.0 6 1120 19 0 0 0 0 2 149 1407 0.0 6 1380 23 2 1 0 0 1 150 1730 0.0 6 1703 22 2 1 1 0 1 151 1192 0.0 6 1169 23 152 1574 0.0 6 1547 25 1 0 1 153 1579 0.0 6 1554 21 2 0 1 0 1 154 1327 0.0 6 1301 23 1 0 2 155 1389 0.0 6 1371 13 2 1 1 0 1 156 1264 0.0 6 1235 24 0 3 1 1 157 1445 0.0 6 1426 16 0 2 1 158 1499 0.0 6 1475 19 0 0 1 2 2 159 1428 0.0 6 1412 13 1 0 2 160 1229 0.0 6 1218 9 1 0 0 1 161 1092 0.0 6 1079 11 1 0 1 162 984 0.0 6 968 16 163 1090 0.0 6 1076 11 2 0 0 1 164 1076 0.0 6 1057 15 1 1 2 165 1115 0.0 6 1099 16 166 1265 0.0 6 1241 21 2 0 0 0 1 167 1015 0.0 6 1002 13 168 1471 0.0 6 1446 23 1 0 1 169 997 0.0 6 982 15 170 1135 0.0 6 1113 21 0 0 0 0 1 171 1263 0.0 6 1239 22 1 0 0 1 172 1041 0.0 6 1022 16 0 2 1 173 1338 0.0 6 1316 22 174 1443 0.0 6 1416 24 2 0 0 0 1 175 1587 0.0 6 1555 28 2 0 1 1 176 1488 0.0 6 1463 23 1 0 0 1 177 1247 0.0 6 1219 26 1 0 0 1 178 1054 0.0 6 1036 17 1 179 932 0.0 6 912 17 0 1 2 180 953 0.0 6 936 11 2 1 0 1 2 181 810 0.0 6 792 18 182 685 0.0 6 676 9 183 1091 0.0 6 1075 16 184 1221 0.0 6 1197 22 2 185 1357 0.0 6 1327 27 1 1 0 1 186 1410 0.0 6 1377 29 3 0 1 187 1253 0.0 6 1222 30 1 188 1233 0.0 6 1216 17 189 1028 0.0 6 999 26 3 190 1254 0.0 6 1219 32 0 1 2 191 1292 0.0 6 1259 32 1 192 939 0.0 6 916 22 0 0 0 0 1 193 807 0.0 6 785 21 0 0 1 194 823 0.0 6 804 18 1 195 1379 0.0 6 1348 31 196 1130 0.0 6 1091 37 1 1 197 1013 0.0 6 973 39 1 198 1568 0.0 6 1524 40 2 0 1 1 199 1230 0.0 6 1196 32 0 0 2 200 1254 0.0 6 1228 24 2 201 844 0.0 6 811 26 3 3 1 202 637 0.0 6 623 12 1 0 1 203 603 0.0 6 579 23 0 1 204 535 0.0 6 515 19 0 0 0 1 205 571 0.0 6 553 18 206 318 0.0 6 304 12 0 1 0 1 207 417 0.0 6 407 10 208 434 0.0 6 423 11 209 372 0.0 6 359 11 0 0 2 210 292 0.0 6 276 15 1 211 856 0.0 6 834 21 0 0 1 212 497 0.0 6 480 15 2 213 539 0.0 6 511 27 0 0 0 0 1 214 1013 0.0 6 984 29 215 533 0.0 6 514 18 1 216 698 0.0 6 674 22 1 0 0 0 1 217 400 0.0 6 389 11 218 375 0.0 6 361 14 219 305 0.0 6 296 8 0 1 220 225 0.0 6 220 5 221 445 0.0 6 430 12 1 1 1 222 535 0.0 6 517 17 0 1 223 428 0.0 6 413 14 0 0 1 224 529 0.0 6 514 14 1 225 363 0.0 6 351 11 1 226 389 0.0 6 377 12 227 464 0.0 6 454 10 228 363 0.0 6 347 15 1 229 636 0.0 6 616 20 230 205 0.0 6 199 6 231 310 0.0 6 298 10 1 0 1 232 283 0.0 6 268 13 0 1 1 233 183 0.0 6 172 11 234 246 0.0 6 240 6 235 167 0.0 6 161 5 0 0 0 0 1 236 68 0.0 6 66 2 237 53 0.0 6 51 2 238 126 0.0 6 118 6 1 1 239 164 0.0 6 162 2 240 155 0.0 6 151 4 241 333 0.0 6 326 7 242 212 0.0 6 200 11 0 0 1 243 134 0.0 6 131 3 244 108 0.0 6 103 5 245 67 0.0 6 64 3 246 76 0.0 6 75 1 247 75 0.0 6 73 2 248 138 0.0 6 136 2 249 52 0.0 6 51 1 250 43 0.0 6 43 251 97 0.0 6 96 1 252 19 0.0 6 17 2 253 18 0.0 6 18 254 1 0.0 6 1 257 1 0.0 6 1 258 1 0.0 6 1 263 3 0.0 6 3 267 1 0.0 6 1 275 1 0.0 6 1 276 3 0.0 6 3 277 4 0.0 6 3 1 278 15 0.0 6 14 1 279 125 0.0 6 124 1 280 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1529 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 49 1 0.0 6 0 1 58 1 0.0 6 1 68 1 0.0 6 1 70 1 0.0 6 1 71 1 0.0 6 1 72 4 0.0 6 4 73 6 0.0 6 6 74 15 0.0 6 15 75 11 0.0 6 11 76 13 0.0 6 13 77 16 0.0 6 16 78 16 0.0 6 16 79 4 0.0 6 4 80 27 0.0 6 26 1 81 6 0.0 6 6 82 14 0.0 6 14 83 8 0.0 6 8 84 8 0.0 6 8 85 12 0.0 6 10 0 1 0 1 86 6 0.0 6 6 87 4 0.0 6 4 88 7 0.0 6 7 89 9 0.0 6 9 90 8 0.0 6 8 91 10 0.0 6 10 92 14 0.0 6 14 93 11 0.0 6 11 94 11 0.0 6 11 95 17 0.0 6 17 96 5 0.0 6 5 97 4 0.0 6 4 98 8 0.0 6 8 99 8 0.0 6 8 100 5 0.0 6 5 101 9 0.0 6 9 102 24 0.0 6 24 103 5 0.0 6 5 104 14 0.0 6 14 105 8 0.0 6 8 106 12 0.0 6 12 107 12 0.0 6 12 108 9 0.0 6 9 109 25 0.0 6 25 110 47 0.0 6 46 1 111 42 0.0 6 42 112 7 0.0 6 7 113 12 0.0 6 12 114 10 0.0 6 10 115 9 0.0 6 9 116 11 0.0 6 11 117 17 0.0 6 17 118 7 0.0 6 7 119 18 0.0 6 18 120 18 0.0 6 18 121 9 0.0 6 8 0 0 0 1 122 7 0.0 6 7 123 9 0.0 6 9 124 13 0.0 6 12 0 0 0 1 125 25 0.0 6 25 126 16 0.0 6 16 127 7 0.0 6 7 128 25 0.0 6 25 129 4 0.0 6 4 130 13 0.0 6 13 131 15 0.0 6 14 0 0 1 132 7 0.0 6 7 133 14 0.0 6 14 134 20 0.0 6 20 135 7 0.0 6 7 136 8 0.0 6 8 137 13 0.0 6 13 138 7 0.0 6 7 139 9 0.0 6 9 140 9 0.0 6 9 141 12 0.0 6 12 142 6 0.0 6 6 143 12 0.0 6 12 144 10 0.0 6 10 145 13 0.0 6 13 146 22 0.0 6 22 147 9 0.0 6 9 148 7 0.0 6 7 149 22 0.0 6 22 150 28 0.0 6 28 151 16 0.0 6 16 152 20 0.0 6 20 153 8 0.0 6 8 154 9 0.0 6 8 0 0 0 1 155 7 0.0 6 7 156 11 0.0 6 10 0 0 0 1 157 10 0.0 6 10 158 14 0.0 6 12 2 159 16 0.0 6 16 160 8 0.0 6 8 161 27 0.0 6 27 162 26 0.0 6 26 163 10 0.0 6 10 164 14 0.0 6 14 165 14 0.0 6 14 166 15 0.0 6 15 167 33 0.0 6 33 168 2 0.0 6 2 169 17 0.0 6 17 170 16 0.0 6 15 0 0 1 171 4 0.0 6 4 172 15 0.0 6 15 173 8 0.0 6 8 174 12 0.0 6 12 175 6 0.0 6 6 176 9 0.0 6 9 177 10 0.0 6 10 178 13 0.0 6 13 179 5 0.0 6 5 180 7 0.0 6 7 181 3 0.0 6 3 182 8 0.0 6 7 1 183 4 0.0 6 4 184 6 0.0 6 6 185 8 0.0 6 8 186 15 0.0 6 15 187 13 0.0 6 13 188 8 0.0 6 8 189 4 0.0 6 4 190 5 0.0 6 5 191 6 0.0 6 6 192 3 0.0 6 3 193 5 0.0 6 5 194 8 0.0 6 8 195 8 0.0 6 8 196 2 0.0 6 2 197 5 0.0 6 4 0 0 0 1 198 6 0.0 6 6 199 4 0.0 6 4 200 2 0.0 6 2 201 5 0.0 6 5 202 4 0.0 6 4 203 5 0.0 6 5 204 3 0.0 6 3 205 2 0.0 6 2 206 2 0.0 6 2 208 1 0.0 6 1 211 1 0.0 6 1 212 1 0.0 6 1 213 2 0.0 6 2 214 3 0.0 6 3 215 1 0.0 6 1 216 2 0.0 6 2 217 2 0.0 6 2 223 1 0.0 6 1 225 1 0.0 6 1 227 1 0.0 6 1 232 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_08_200_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_08_427_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_08_200_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_08_427_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.00 s (71 µs/read; 0.84 M reads/minute). === Summary === Total read pairs processed: 70,051 Read 1 with adapter: 70,045 (100.0%) Read 2 with adapter: 70,051 (100.0%) == Read fate breakdown == Pairs that were too short: 25 (0.0%) Pairs discarded as untrimmed: 6 (0.0%) Pairs written (passing filters): 70,020 (100.0%) Total basepairs processed: 28,153,618 bp Read 1: 15,384,749 bp Read 2: 12,768,869 bp Total written (filtered): 4,511,835 bp (16.0%) Read 1: 2,229,453 bp Read 2: 2,282,382 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 67852 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1094.5 0 0 1 4 1 273.6 1 0 1 6 2 17.1 1 1 0 1 7 2 4.3 2 1 0 1 11 1 0.0 3 1 14 3 0.0 4 3 15 7 0.0 4 5 2 16 1 0.0 4 0 1 17 1 0.0 5 1 18 1 0.0 5 1 19 1 0.0 5 1 20 119 0.0 6 118 1 21 68 0.0 6 67 1 22 52 0.0 6 52 23 35 0.0 6 35 24 27 0.0 6 27 25 43 0.0 6 42 1 26 21 0.0 6 19 2 27 17 0.0 6 16 1 28 11 0.0 6 11 29 6 0.0 6 6 30 6 0.0 6 6 31 10 0.0 6 10 32 6 0.0 6 6 33 8 0.0 6 8 34 13 0.0 6 13 35 4 0.0 6 4 36 11 0.0 6 10 1 37 9 0.0 6 9 38 10 0.0 6 9 1 39 11 0.0 6 10 1 40 11 0.0 6 10 1 41 9 0.0 6 9 42 5 0.0 6 5 43 5 0.0 6 5 44 7 0.0 6 5 2 45 3 0.0 6 3 46 4 0.0 6 4 47 7 0.0 6 7 48 4 0.0 6 4 49 8 0.0 6 8 50 2 0.0 6 2 51 9 0.0 6 8 0 1 52 14 0.0 6 14 53 11 0.0 6 9 2 54 10 0.0 6 10 55 4 0.0 6 4 56 3 0.0 6 3 57 5 0.0 6 5 58 5 0.0 6 4 0 1 59 3 0.0 6 3 60 4 0.0 6 4 61 3 0.0 6 3 62 7 0.0 6 5 2 63 4 0.0 6 4 64 8 0.0 6 8 65 8 0.0 6 8 66 5 0.0 6 5 67 6 0.0 6 6 68 6 0.0 6 6 69 11 0.0 6 10 1 70 5 0.0 6 5 71 13 0.0 6 13 72 14 0.0 6 14 73 16 0.0 6 16 74 12 0.0 6 12 75 11 0.0 6 11 76 8 0.0 6 7 1 77 18 0.0 6 18 78 12 0.0 6 12 79 12 0.0 6 12 80 3 0.0 6 3 81 7 0.0 6 7 82 11 0.0 6 11 83 12 0.0 6 12 84 11 0.0 6 11 85 11 0.0 6 10 1 86 14 0.0 6 13 1 87 17 0.0 6 16 1 88 10 0.0 6 10 89 7 0.0 6 6 1 90 9 0.0 6 9 91 9 0.0 6 9 92 10 0.0 6 10 93 8 0.0 6 8 94 15 0.0 6 15 95 12 0.0 6 12 96 10 0.0 6 10 97 10 0.0 6 9 1 98 6 0.0 6 6 99 17 0.0 6 17 100 12 0.0 6 12 101 9 0.0 6 8 1 102 15 0.0 6 15 103 13 0.0 6 12 1 104 32 0.0 6 32 105 24 0.0 6 23 1 106 25 0.0 6 25 107 28 0.0 6 28 108 25 0.0 6 25 109 32 0.0 6 32 110 29 0.0 6 29 111 28 0.0 6 28 112 47 0.0 6 47 113 44 0.0 6 42 1 0 1 114 28 0.0 6 28 115 30 0.0 6 28 1 0 0 0 1 116 44 0.0 6 44 117 31 0.0 6 31 118 39 0.0 6 37 0 0 0 0 1 1 119 39 0.0 6 34 1 0 0 0 4 120 28 0.0 6 27 1 121 36 0.0 6 34 1 0 0 0 0 1 122 32 0.0 6 32 123 31 0.0 6 31 124 32 0.0 6 32 125 39 0.0 6 39 126 47 0.0 6 47 127 54 0.0 6 53 1 128 59 0.0 6 59 129 43 0.0 6 43 130 50 0.0 6 50 131 60 0.0 6 59 1 132 53 0.0 6 52 1 133 66 0.0 6 64 2 134 53 0.0 6 53 135 63 0.0 6 62 1 136 67 0.0 6 66 1 137 86 0.0 6 85 1 138 163 0.0 6 158 5 139 121 0.0 6 121 140 113 0.0 6 111 2 141 148 0.0 6 148 142 424 0.0 6 424 143 830 0.0 6 827 3 144 1068 0.0 6 1066 2 145 1002 0.0 6 998 3 0 0 0 0 1 146 1016 0.0 6 1016 147 1109 0.0 6 1106 2 1 148 1311 0.0 6 1307 4 149 1219 0.0 6 1216 1 0 2 150 1191 0.0 6 1190 1 151 1158 0.0 6 1155 2 0 1 152 1669 0.0 6 1667 1 0 0 0 1 153 882 0.0 6 882 154 705 0.0 6 704 1 155 650 0.0 6 650 156 756 0.0 6 755 1 157 564 0.0 6 562 2 158 541 0.0 6 539 1 0 0 0 0 1 159 544 0.0 6 543 1 160 587 0.0 6 585 2 161 757 0.0 6 755 2 162 623 0.0 6 622 1 163 542 0.0 6 541 1 164 453 0.0 6 453 165 726 0.0 6 724 2 166 521 0.0 6 519 1 0 0 0 0 1 167 398 0.0 6 395 3 168 391 0.0 6 390 1 169 409 0.0 6 403 6 170 518 0.0 6 516 2 171 547 0.0 6 546 1 172 339 0.0 6 338 1 173 549 0.0 6 548 0 1 174 520 0.0 6 519 0 0 0 0 0 1 175 477 0.0 6 477 176 343 0.0 6 340 3 177 487 0.0 6 486 1 178 370 0.0 6 368 2 179 376 0.0 6 374 1 0 0 0 0 1 180 754 0.0 6 752 1 1 181 398 0.0 6 396 1 1 182 356 0.0 6 356 183 404 0.0 6 402 2 184 279 0.0 6 276 2 0 0 1 185 500 0.0 6 495 4 0 0 0 1 186 542 0.0 6 537 5 187 478 0.0 6 477 1 188 364 0.0 6 363 1 189 480 0.0 6 479 1 190 414 0.0 6 414 191 468 0.0 6 467 1 192 365 0.0 6 363 2 193 550 0.0 6 549 1 194 406 0.0 6 403 3 195 386 0.0 6 384 2 196 496 0.0 6 491 4 0 1 197 554 0.0 6 549 5 198 614 0.0 6 610 4 199 548 0.0 6 545 3 200 709 0.0 6 704 4 0 0 0 0 1 201 598 0.0 6 591 6 0 0 0 0 1 202 514 0.0 6 513 1 203 451 0.0 6 449 2 204 469 0.0 6 468 1 205 449 0.0 6 448 1 206 478 0.0 6 476 2 207 536 0.0 6 531 4 0 1 208 686 0.0 6 682 3 0 0 0 1 209 577 0.0 6 571 4 1 0 0 1 210 681 0.0 6 676 4 0 0 0 0 1 211 505 0.0 6 499 6 212 428 0.0 6 424 4 213 493 0.0 6 488 4 0 1 214 609 0.0 6 603 6 215 582 0.0 6 580 2 216 689 0.0 6 683 5 0 0 0 1 217 824 0.0 6 824 218 590 0.0 6 585 5 219 630 0.0 6 629 1 220 626 0.0 6 621 2 2 0 0 0 1 221 541 0.0 6 538 1 0 0 1 1 222 482 0.0 6 480 2 223 509 0.0 6 507 1 1 224 451 0.0 6 447 4 225 510 0.0 6 509 1 226 476 0.0 6 474 2 227 564 0.0 6 557 6 0 0 0 0 1 228 543 0.0 6 541 0 1 0 0 0 1 229 419 0.0 6 417 1 0 0 0 0 1 230 458 0.0 6 454 4 231 450 0.0 6 446 3 0 0 1 232 427 0.0 6 427 233 455 0.0 6 453 1 1 234 434 0.0 6 431 3 235 418 0.0 6 416 1 0 0 0 0 1 236 356 0.0 6 355 1 237 341 0.0 6 337 4 238 385 0.0 6 385 239 353 0.0 6 350 3 240 361 0.0 6 358 2 0 1 241 336 0.0 6 334 1 0 0 0 0 1 242 307 0.0 6 305 2 243 330 0.0 6 330 244 369 0.0 6 368 1 245 279 0.0 6 278 1 246 288 0.0 6 283 5 247 238 0.0 6 237 0 0 0 0 0 1 248 168 0.0 6 167 1 249 171 0.0 6 171 250 207 0.0 6 206 1 251 214 0.0 6 214 252 184 0.0 6 180 4 253 181 0.0 6 180 1 254 164 0.0 6 163 1 255 184 0.0 6 183 1 256 151 0.0 6 150 1 257 138 0.0 6 138 258 127 0.0 6 127 259 127 0.0 6 127 260 149 0.0 6 149 261 167 0.0 6 165 0 0 1 0 0 1 262 120 0.0 6 120 263 96 0.0 6 95 1 264 104 0.0 6 104 265 87 0.0 6 86 1 266 85 0.0 6 85 267 102 0.0 6 101 1 268 95 0.0 6 95 269 100 0.0 6 100 270 94 0.0 6 94 271 108 0.0 6 108 272 71 0.0 6 69 2 273 62 0.0 6 62 274 68 0.0 6 66 2 275 72 0.0 6 72 276 103 0.0 6 102 1 277 102 0.0 6 102 278 139 0.0 6 138 1 279 195 0.0 6 194 1 280 618 0.0 6 614 4 281 918 0.0 6 910 7 0 0 0 1 282 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2193 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 50 4 0.0 6 4 60 1 0.0 6 0 1 61 221 0.0 6 213 8 62 3 0.0 6 3 63 45 0.0 6 43 2 67 60 0.0 6 60 68 4 0.0 6 3 1 69 2 0.0 6 2 70 21 0.0 6 21 71 40 0.0 6 40 72 31 0.0 6 31 73 31 0.0 6 31 74 31 0.0 6 31 75 42 0.0 6 42 76 39 0.0 6 38 1 77 43 0.0 6 43 78 37 0.0 6 37 79 43 0.0 6 42 1 80 59 0.0 6 59 81 43 0.0 6 43 82 18 0.0 6 18 83 37 0.0 6 37 84 29 0.0 6 28 1 85 17 0.0 6 16 0 0 0 0 0 1 86 14 0.0 6 14 87 15 0.0 6 15 88 28 0.0 6 28 89 18 0.0 6 18 90 15 0.0 6 15 91 23 0.0 6 23 92 8 0.0 6 8 93 13 0.0 6 13 94 13 0.0 6 13 95 10 0.0 6 10 96 13 0.0 6 13 97 9 0.0 6 9 98 11 0.0 6 11 99 8 0.0 6 8 100 11 0.0 6 11 101 20 0.0 6 20 102 14 0.0 6 14 103 13 0.0 6 13 104 9 0.0 6 9 105 24 0.0 6 24 106 8 0.0 6 8 107 16 0.0 6 16 108 21 0.0 6 21 109 7 0.0 6 7 110 12 0.0 6 12 111 10 0.0 6 10 112 9 0.0 6 9 113 11 0.0 6 11 114 10 0.0 6 10 115 9 0.0 6 9 116 9 0.0 6 9 117 10 0.0 6 10 118 9 0.0 6 9 119 11 0.0 6 11 120 5 0.0 6 5 121 7 0.0 6 7 122 7 0.0 6 7 123 5 0.0 6 5 124 12 0.0 6 12 125 10 0.0 6 10 126 5 0.0 6 5 127 6 0.0 6 6 128 6 0.0 6 6 129 3 0.0 6 3 130 10 0.0 6 10 131 4 0.0 6 4 132 4 0.0 6 4 133 2 0.0 6 2 134 7 0.0 6 7 135 8 0.0 6 8 136 9 0.0 6 9 137 11 0.0 6 11 138 4 0.0 6 4 139 8 0.0 6 8 140 5 0.0 6 5 141 8 0.0 6 8 142 7 0.0 6 6 0 0 0 0 0 1 143 12 0.0 6 12 144 10 0.0 6 10 145 4 0.0 6 4 146 8 0.0 6 8 147 17 0.0 6 17 148 8 0.0 6 8 149 8 0.0 6 8 151 5 0.0 6 5 152 4 0.0 6 4 153 8 0.0 6 8 154 5 0.0 6 5 155 13 0.0 6 13 156 5 0.0 6 5 157 10 0.0 6 10 158 14 0.0 6 14 159 10 0.0 6 10 160 7 0.0 6 7 161 16 0.0 6 16 162 8 0.0 6 8 163 8 0.0 6 8 164 5 0.0 6 5 165 4 0.0 6 4 166 7 0.0 6 7 167 3 0.0 6 3 168 8 0.0 6 8 169 6 0.0 6 6 170 1 0.0 6 1 171 11 0.0 6 11 172 5 0.0 6 5 173 8 0.0 6 8 174 5 0.0 6 5 175 11 0.0 6 11 176 11 0.0 6 11 177 8 0.0 6 8 178 10 0.0 6 10 179 11 0.0 6 11 180 5 0.0 6 5 181 3 0.0 6 3 182 6 0.0 6 6 183 15 0.0 6 15 184 10 0.0 6 10 185 6 0.0 6 6 186 11 0.0 6 11 187 9 0.0 6 9 188 5 0.0 6 5 189 4 0.0 6 4 190 5 0.0 6 5 191 7 0.0 6 7 192 6 0.0 6 6 193 3 0.0 6 3 194 4 0.0 6 4 195 9 0.0 6 8 1 196 10 0.0 6 10 197 2 0.0 6 2 198 5 0.0 6 5 199 7 0.0 6 7 200 10 0.0 6 10 201 8 0.0 6 8 202 11 0.0 6 11 203 4 0.0 6 4 204 8 0.0 6 8 205 12 0.0 6 12 206 9 0.0 6 9 207 10 0.0 6 10 208 5 0.0 6 5 209 5 0.0 6 5 210 7 0.0 6 7 211 3 0.0 6 3 212 4 0.0 6 4 213 4 0.0 6 4 214 5 0.0 6 5 215 9 0.0 6 9 216 5 0.0 6 5 217 2 0.0 6 2 218 4 0.0 6 4 219 6 0.0 6 6 220 4 0.0 6 4 221 8 0.0 6 8 222 9 0.0 6 9 223 2 0.0 6 2 224 6 0.0 6 6 225 4 0.0 6 4 226 3 0.0 6 3 227 2 0.0 6 2 228 4 0.0 6 4 229 3 0.0 6 3 230 5 0.0 6 5 231 6 0.0 6 6 232 7 0.0 6 7 233 1 0.0 6 1 234 9 0.0 6 9 235 1 0.0 6 1 236 1 0.0 6 1 237 1 0.0 6 1 238 4 0.0 6 4 239 3 0.0 6 3 241 1 0.0 6 1 244 7 0.0 6 7 246 2 0.0 6 2 247 4 0.0 6 4 248 1 0.0 6 1 249 4 0.0 6 4 250 3 0.0 6 3 251 1 0.0 6 1 253 1 0.0 6 1 255 3 0.0 6 3 256 2 0.0 6 2 257 1 0.0 6 1 258 1 0.0 6 1 259 3 0.0 6 3 262 3 0.0 6 3 263 1 0.0 6 1 264 1 0.0 6 1 265 1 0.0 6 1 266 1 0.0 6 1 267 1 0.0 6 1 269 1 0.0 6 1 273 2 0.0 6 2 274 4 0.0 6 4 275 1 0.0 6 1 278 1 0.0 6 1 280 2 0.0 6 2 283 1 0.0 6 1 285 1 0.0 6 1 289 1 0.0 6 1 293 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 67489 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 2 4.3 2 1 1 8 1 1.1 2 0 0 0 1 9 1 0.3 2 1 10 2 0.1 3 2 11 4 0.0 3 4 12 7 0.0 3 7 13 8 0.0 3 7 1 14 8 0.0 4 8 15 5 0.0 4 1 4 16 10 0.0 4 8 2 17 28 0.0 5 27 0 1 18 17 0.0 5 15 2 19 9 0.0 5 8 1 20 69 0.0 6 62 7 21 47 0.0 6 45 1 1 22 24 0.0 6 21 2 1 23 20 0.0 6 17 2 1 24 17 0.0 6 15 2 25 12 0.0 6 11 0 1 26 8 0.0 6 6 2 27 13 0.0 6 10 1 1 1 28 17 0.0 6 16 1 29 15 0.0 6 12 1 2 30 8 0.0 6 7 1 31 17 0.0 6 16 1 32 7 0.0 6 6 0 1 33 18 0.0 6 16 2 34 10 0.0 6 10 35 7 0.0 6 7 36 16 0.0 6 12 3 1 37 15 0.0 6 14 1 38 16 0.0 6 16 39 11 0.0 6 10 1 40 25 0.0 6 23 2 41 17 0.0 6 17 42 11 0.0 6 10 1 43 17 0.0 6 15 2 44 11 0.0 6 9 2 45 10 0.0 6 9 1 46 20 0.0 6 20 47 15 0.0 6 12 3 48 16 0.0 6 15 1 49 18 0.0 6 17 1 50 25 0.0 6 24 1 51 17 0.0 6 15 2 52 27 0.0 6 26 1 53 30 0.0 6 26 3 0 0 0 1 54 19 0.0 6 16 3 55 20 0.0 6 15 4 0 1 56 20 0.0 6 19 1 57 19 0.0 6 19 58 19 0.0 6 17 2 59 39 0.0 6 37 1 0 0 1 60 25 0.0 6 22 0 0 0 1 2 61 33 0.0 6 29 4 62 34 0.0 6 33 1 63 28 0.0 6 27 1 64 49 0.0 6 43 3 2 1 65 28 0.0 6 27 1 66 36 0.0 6 35 1 67 57 0.0 6 53 4 68 139 0.0 6 136 1 1 1 69 33 0.0 6 33 70 79 0.0 6 75 3 1 71 34 0.0 6 31 3 72 54 0.0 6 51 2 0 0 0 1 73 43 0.0 6 40 3 74 46 0.0 6 45 1 75 52 0.0 6 51 0 1 76 38 0.0 6 37 1 77 42 0.0 6 41 1 78 43 0.0 6 41 0 0 1 0 0 1 79 39 0.0 6 38 1 80 51 0.0 6 48 3 81 69 0.0 6 68 0 0 0 1 82 54 0.0 6 50 4 83 61 0.0 6 60 1 84 90 0.0 6 87 2 1 85 38 0.0 6 37 1 86 48 0.0 6 46 2 87 47 0.0 6 43 3 0 0 0 1 88 55 0.0 6 54 1 89 55 0.0 6 53 2 90 52 0.0 6 48 4 91 64 0.0 6 64 92 71 0.0 6 68 2 0 1 93 71 0.0 6 67 4 94 96 0.0 6 94 2 95 77 0.0 6 74 3 96 85 0.0 6 85 97 91 0.0 6 90 1 98 88 0.0 6 86 2 99 82 0.0 6 78 2 0 2 100 72 0.0 6 71 1 101 96 0.0 6 93 3 102 79 0.0 6 77 2 103 111 0.0 6 108 3 104 124 0.0 6 121 2 0 0 0 1 105 179 0.0 6 176 3 106 108 0.0 6 104 4 107 94 0.0 6 92 1 0 0 0 1 108 99 0.0 6 95 4 109 129 0.0 6 129 110 134 0.0 6 127 6 1 111 140 0.0 6 137 2 0 0 1 112 184 0.0 6 179 4 1 113 191 0.0 6 188 3 114 149 0.0 6 148 1 115 166 0.0 6 162 4 116 176 0.0 6 175 1 117 160 0.0 6 155 5 118 236 0.0 6 235 1 119 202 0.0 6 200 2 120 195 0.0 6 191 4 121 237 0.0 6 232 5 122 212 0.0 6 212 123 216 0.0 6 214 1 0 0 0 1 124 261 0.0 6 259 2 125 308 0.0 6 304 3 1 126 327 0.0 6 321 6 127 329 0.0 6 321 8 128 376 0.0 6 370 6 129 490 0.0 6 486 4 130 405 0.0 6 396 9 131 443 0.0 6 432 10 0 0 1 132 466 0.0 6 459 5 0 0 1 1 133 455 0.0 6 448 7 134 516 0.0 6 508 7 1 135 627 0.0 6 615 12 136 645 0.0 6 640 5 137 551 0.0 6 540 7 0 2 1 1 138 775 0.0 6 770 3 0 0 1 1 139 1120 0.0 6 1115 5 140 1307 0.0 6 1300 3 4 141 1242 0.0 6 1230 9 1 0 2 142 1373 0.0 6 1363 8 1 1 143 1670 0.0 6 1660 9 0 1 144 1518 0.0 6 1505 8 2 2 1 145 1644 0.0 6 1628 12 1 0 0 0 3 146 1462 0.0 6 1451 10 0 0 0 1 147 1480 0.0 6 1468 10 0 0 1 1 148 1926 0.0 6 1915 8 0 0 1 2 149 1478 0.0 6 1464 13 0 1 150 1336 0.0 6 1317 16 1 1 0 1 151 1684 0.0 6 1673 11 152 1465 0.0 6 1452 13 153 1514 0.0 6 1494 18 0 0 0 0 2 154 1492 0.0 6 1467 21 0 0 2 0 2 155 1179 0.0 6 1170 8 1 156 1310 0.0 6 1294 13 2 0 1 157 1200 0.0 6 1190 9 0 0 0 1 158 1205 0.0 6 1192 13 159 1245 0.0 6 1236 7 1 0 0 1 160 962 0.0 6 956 5 0 1 161 1077 0.0 6 1066 10 0 0 0 0 1 162 843 0.0 6 833 8 1 0 0 0 1 163 815 0.0 6 798 13 2 0 1 1 164 815 0.0 6 806 7 2 165 807 0.0 6 793 10 0 0 0 2 2 166 779 0.0 6 776 3 167 866 0.0 6 854 10 0 1 0 1 168 696 0.0 6 686 6 1 0 1 0 2 169 962 0.0 6 957 3 0 0 0 2 170 766 0.0 6 756 7 2 0 0 1 171 777 0.0 6 776 1 172 865 0.0 6 856 8 0 0 1 173 784 0.0 6 779 5 174 689 0.0 6 684 5 175 714 0.0 6 708 5 0 0 0 0 1 176 741 0.0 6 732 8 1 177 651 0.0 6 648 3 178 481 0.0 6 474 7 179 553 0.0 6 543 9 0 0 1 180 401 0.0 6 401 181 380 0.0 6 376 4 182 378 0.0 6 371 5 1 1 183 353 0.0 6 348 4 0 0 1 184 495 0.0 6 490 4 0 0 0 1 185 668 0.0 6 664 3 0 0 0 1 186 597 0.0 6 592 4 0 0 0 0 1 187 354 0.0 6 349 2 0 1 1 0 1 188 338 0.0 6 334 3 0 0 0 0 1 189 314 0.0 6 311 3 190 287 0.0 6 284 3 191 248 0.0 6 244 3 0 1 192 280 0.0 6 278 2 193 218 0.0 6 213 4 0 0 0 1 194 171 0.0 6 169 2 195 186 0.0 6 184 2 196 175 0.0 6 171 3 0 1 197 235 0.0 6 229 5 0 0 1 198 163 0.0 6 162 1 199 165 0.0 6 161 3 1 200 144 0.0 6 143 1 201 152 0.0 6 151 1 202 94 0.0 6 93 1 203 93 0.0 6 93 204 89 0.0 6 89 205 76 0.0 6 75 1 206 81 0.0 6 79 1 0 0 0 0 1 207 87 0.0 6 86 0 0 0 0 1 208 73 0.0 6 73 209 65 0.0 6 64 1 210 72 0.0 6 72 211 47 0.0 6 46 0 1 212 78 0.0 6 78 213 53 0.0 6 52 1 214 50 0.0 6 50 215 55 0.0 6 53 2 216 43 0.0 6 43 217 40 0.0 6 39 0 1 218 34 0.0 6 34 219 36 0.0 6 36 220 35 0.0 6 35 221 23 0.0 6 23 222 33 0.0 6 33 223 31 0.0 6 30 1 224 20 0.0 6 20 225 23 0.0 6 23 226 11 0.0 6 11 227 12 0.0 6 12 228 6 0.0 6 6 229 8 0.0 6 8 230 9 0.0 6 9 231 6 0.0 6 6 232 7 0.0 6 6 1 233 4 0.0 6 4 234 7 0.0 6 7 235 4 0.0 6 4 236 5 0.0 6 5 237 3 0.0 6 3 238 3 0.0 6 3 239 2 0.0 6 2 240 5 0.0 6 5 241 3 0.0 6 3 242 1 0.0 6 1 245 1 0.0 6 1 247 1 0.0 6 1 250 1 0.0 6 1 252 1 0.0 6 1 278 1 0.0 6 1 279 8 0.0 6 8 280 50 0.0 6 48 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2562 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 6 62 17.1 1 0 0 62 47 1 0.0 6 0 0 1 48 6 0.0 6 0 6 56 8 0.0 6 7 1 57 15 0.0 6 15 58 7 0.0 6 7 62 8 0.0 6 8 65 2 0.0 6 2 66 22 0.0 6 18 4 67 152 0.0 6 149 3 68 4 0.0 6 4 69 18 0.0 6 17 1 70 29 0.0 6 29 71 38 0.0 6 38 72 69 0.0 6 69 73 46 0.0 6 45 1 74 94 0.0 6 94 75 50 0.0 6 49 0 0 0 1 76 46 0.0 6 44 1 0 0 1 77 63 0.0 6 63 78 62 0.0 6 62 79 48 0.0 6 48 80 57 0.0 6 56 0 1 81 92 0.0 6 92 82 81 0.0 6 81 83 30 0.0 6 30 84 44 0.0 6 44 85 38 0.0 6 38 86 27 0.0 6 26 1 87 19 0.0 6 19 88 33 0.0 6 33 89 30 0.0 6 29 1 90 29 0.0 6 29 91 28 0.0 6 28 92 19 0.0 6 19 93 16 0.0 6 16 94 37 0.0 6 36 0 1 95 25 0.0 6 25 96 19 0.0 6 19 97 15 0.0 6 15 98 24 0.0 6 24 99 20 0.0 6 20 100 29 0.0 6 29 101 16 0.0 6 16 102 27 0.0 6 27 103 20 0.0 6 20 104 14 0.0 6 14 105 11 0.0 6 11 106 15 0.0 6 15 107 14 0.0 6 14 108 13 0.0 6 13 109 29 0.0 6 29 110 12 0.0 6 12 111 11 0.0 6 11 112 13 0.0 6 12 0 0 0 1 113 12 0.0 6 11 1 114 19 0.0 6 19 115 18 0.0 6 18 116 15 0.0 6 15 117 8 0.0 6 8 118 12 0.0 6 12 119 15 0.0 6 15 120 17 0.0 6 17 121 11 0.0 6 11 122 11 0.0 6 11 123 8 0.0 6 8 124 6 0.0 6 6 125 14 0.0 6 14 126 10 0.0 6 10 127 11 0.0 6 11 128 10 0.0 6 10 129 5 0.0 6 5 130 13 0.0 6 13 131 15 0.0 6 15 132 4 0.0 6 4 133 12 0.0 6 12 134 11 0.0 6 10 1 135 9 0.0 6 9 136 10 0.0 6 10 137 9 0.0 6 9 138 10 0.0 6 10 139 8 0.0 6 8 140 9 0.0 6 9 141 5 0.0 6 5 142 8 0.0 6 8 143 2 0.0 6 2 144 12 0.0 6 12 145 6 0.0 6 6 146 6 0.0 6 6 147 12 0.0 6 12 148 13 0.0 6 13 149 13 0.0 6 12 0 0 1 150 6 0.0 6 6 151 3 0.0 6 3 152 7 0.0 6 7 153 4 0.0 6 4 154 10 0.0 6 10 155 14 0.0 6 14 156 11 0.0 6 11 157 12 0.0 6 12 158 17 0.0 6 17 159 10 0.0 6 10 160 15 0.0 6 15 161 8 0.0 6 8 162 17 0.0 6 17 163 11 0.0 6 11 164 10 0.0 6 10 165 14 0.0 6 14 166 7 0.0 6 7 167 5 0.0 6 5 168 3 0.0 6 3 169 5 0.0 6 5 170 5 0.0 6 5 171 7 0.0 6 6 0 0 0 1 172 10 0.0 6 10 173 19 0.0 6 19 174 4 0.0 6 4 175 2 0.0 6 2 176 10 0.0 6 10 177 5 0.0 6 5 178 6 0.0 6 6 179 8 0.0 6 8 180 8 0.0 6 8 181 12 0.0 6 12 182 2 0.0 6 2 183 3 0.0 6 3 184 10 0.0 6 10 185 17 0.0 6 17 186 2 0.0 6 2 187 5 0.0 6 5 188 3 0.0 6 3 189 4 0.0 6 4 190 4 0.0 6 4 191 5 0.0 6 4 1 192 3 0.0 6 3 193 6 0.0 6 6 195 3 0.0 6 3 196 1 0.0 6 1 197 3 0.0 6 3 198 4 0.0 6 4 199 2 0.0 6 2 200 5 0.0 6 5 201 1 0.0 6 1 202 6 0.0 6 6 203 1 0.0 6 1 204 2 0.0 6 2 205 1 0.0 6 1 207 2 0.0 6 2 208 8 0.0 6 8 209 5 0.0 6 5 210 3 0.0 6 3 211 1 0.0 6 1 213 1 0.0 6 1 214 2 0.0 6 2 215 1 0.0 6 1 217 1 0.0 6 1 220 2 0.0 6 2 223 1 0.0 6 1 224 1 0.0 6 1 225 3 0.0 6 3 226 2 0.0 6 2 227 1 0.0 6 1 230 1 0.0 6 1 242 1 0.0 6 1 252 1 0.0 6 1 256 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_09_201_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_09_428_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_09_201_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_09_428_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.28 s (38 µs/read; 1.58 M reads/minute). === Summary === Total read pairs processed: 86,474 Read 1 with adapter: 86,470 (100.0%) Read 2 with adapter: 86,441 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 37 (0.0%) Pairs written (passing filters): 86,437 (100.0%) Total basepairs processed: 44,002,942 bp Read 1: 25,106,169 bp Read 2: 18,896,773 bp Total written (filtered): 5,102,867 bp (11.6%) Read 1: 2,594,666 bp Read 2: 2,508,201 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 86442 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 337.8 1 0 1 5 1 84.4 1 1 6 3 21.1 1 0 0 3 9 2 0.3 2 2 10 1 0.1 3 1 15 1 0.0 4 1 16 1 0.0 4 1 19 1 0.0 5 1 20 10 0.0 6 10 21 5 0.0 6 5 22 3 0.0 6 3 23 1 0.0 6 1 24 4 0.0 6 4 25 5 0.0 6 4 1 26 4 0.0 6 4 27 1 0.0 6 1 28 4 0.0 6 4 29 1 0.0 6 1 30 1 0.0 6 1 33 1 0.0 6 1 34 7 0.0 6 7 35 2 0.0 6 2 36 1 0.0 6 1 38 3 0.0 6 3 39 4 0.0 6 4 40 3 0.0 6 2 0 1 41 2 0.0 6 2 42 1 0.0 6 1 43 1 0.0 6 1 44 6 0.0 6 6 45 4 0.0 6 3 0 1 46 3 0.0 6 2 1 48 1 0.0 6 1 50 1 0.0 6 1 52 1 0.0 6 1 53 2 0.0 6 2 54 3 0.0 6 3 56 4 0.0 6 4 57 5 0.0 6 4 0 1 58 5 0.0 6 5 59 2 0.0 6 2 60 2 0.0 6 2 61 2 0.0 6 1 0 1 62 2 0.0 6 2 63 1 0.0 6 1 64 2 0.0 6 2 65 5 0.0 6 5 67 2 0.0 6 2 68 3 0.0 6 3 69 2 0.0 6 2 70 2 0.0 6 2 71 1 0.0 6 1 72 3 0.0 6 2 0 0 0 1 73 1 0.0 6 0 1 75 2 0.0 6 1 1 77 2 0.0 6 2 78 5 0.0 6 5 79 2 0.0 6 2 80 2 0.0 6 2 81 1 0.0 6 1 82 2 0.0 6 1 0 0 0 1 83 1 0.0 6 0 1 84 1 0.0 6 1 85 1 0.0 6 1 86 9 0.0 6 9 87 3 0.0 6 3 88 2 0.0 6 2 89 6 0.0 6 6 92 1 0.0 6 1 93 3 0.0 6 3 94 1 0.0 6 0 1 95 1 0.0 6 1 97 7 0.0 6 7 98 4 0.0 6 4 100 2 0.0 6 2 101 7 0.0 6 6 1 102 5 0.0 6 5 103 1 0.0 6 1 104 2 0.0 6 2 105 3 0.0 6 3 106 6 0.0 6 4 2 107 8 0.0 6 7 1 108 6 0.0 6 6 109 1 0.0 6 1 110 1 0.0 6 1 111 8 0.0 6 8 112 5 0.0 6 5 113 2 0.0 6 2 114 8 0.0 6 7 1 115 4 0.0 6 4 116 2 0.0 6 2 117 7 0.0 6 7 118 5 0.0 6 5 119 4 0.0 6 4 120 4 0.0 6 4 121 6 0.0 6 6 122 28 0.0 6 26 2 123 7 0.0 6 7 124 13 0.0 6 13 125 23 0.0 6 20 2 0 0 0 0 1 126 6 0.0 6 6 127 5 0.0 6 5 128 24 0.0 6 23 1 129 15 0.0 6 14 1 130 7 0.0 6 7 131 10 0.0 6 9 1 132 22 0.0 6 22 133 11 0.0 6 11 134 16 0.0 6 16 135 18 0.0 6 18 136 17 0.0 6 15 2 137 33 0.0 6 33 138 25 0.0 6 24 1 139 17 0.0 6 17 140 44 0.0 6 44 141 9 0.0 6 8 1 142 16 0.0 6 16 143 56 0.0 6 55 1 144 52 0.0 6 52 145 34 0.0 6 33 1 146 12 0.0 6 12 147 48 0.0 6 48 148 40 0.0 6 39 1 149 34 0.0 6 34 150 29 0.0 6 27 2 151 52 0.0 6 50 2 152 63 0.0 6 63 153 74 0.0 6 74 154 65 0.0 6 64 1 155 57 0.0 6 57 156 38 0.0 6 37 1 157 22 0.0 6 19 2 1 158 39 0.0 6 39 159 41 0.0 6 39 2 160 52 0.0 6 52 161 39 0.0 6 39 162 70 0.0 6 68 1 1 163 40 0.0 6 39 0 1 164 20 0.0 6 19 1 165 23 0.0 6 23 166 52 0.0 6 51 1 167 46 0.0 6 46 168 44 0.0 6 43 1 169 55 0.0 6 53 2 170 60 0.0 6 60 171 86 0.0 6 84 1 1 172 22 0.0 6 20 2 173 50 0.0 6 49 0 0 1 174 65 0.0 6 64 1 175 91 0.0 6 89 2 176 45 0.0 6 45 177 42 0.0 6 42 178 65 0.0 6 62 2 1 179 33 0.0 6 33 180 53 0.0 6 53 181 61 0.0 6 61 182 74 0.0 6 70 4 183 74 0.0 6 70 4 184 91 0.0 6 89 2 185 73 0.0 6 72 1 186 105 0.0 6 102 3 187 79 0.0 6 79 188 74 0.0 6 73 1 189 66 0.0 6 65 1 190 80 0.0 6 79 1 191 96 0.0 6 93 3 192 105 0.0 6 105 193 135 0.0 6 128 7 194 135 0.0 6 134 1 195 104 0.0 6 100 4 196 115 0.0 6 113 2 197 137 0.0 6 133 4 198 155 0.0 6 150 5 199 184 0.0 6 181 3 200 142 0.0 6 139 2 1 201 129 0.0 6 127 1 1 202 124 0.0 6 120 4 203 199 0.0 6 194 4 0 0 1 204 182 0.0 6 181 1 205 223 0.0 6 219 4 206 140 0.0 6 128 12 207 228 0.0 6 225 3 208 299 0.0 6 289 8 2 209 280 0.0 6 273 6 1 210 228 0.0 6 223 4 0 0 0 1 211 342 0.0 6 338 4 212 184 0.0 6 180 4 213 262 0.0 6 256 5 0 0 1 214 431 0.0 6 419 10 2 215 152 0.0 6 147 5 216 412 0.0 6 405 7 217 303 0.0 6 293 10 218 217 0.0 6 214 3 219 192 0.0 6 184 8 220 220 0.0 6 215 5 221 168 0.0 6 162 6 222 264 0.0 6 259 5 223 168 0.0 6 167 1 224 252 0.0 6 246 5 1 225 231 0.0 6 217 12 2 226 715 0.0 6 705 9 1 227 593 0.0 6 570 22 0 1 228 593 0.0 6 576 16 1 229 557 0.0 6 545 12 230 403 0.0 6 390 12 1 231 339 0.0 6 330 9 232 407 0.0 6 393 14 233 351 0.0 6 346 5 234 435 0.0 6 428 7 235 259 0.0 6 251 8 236 223 0.0 6 212 11 237 225 0.0 6 222 3 238 235 0.0 6 228 7 239 298 0.0 6 286 11 0 1 240 966 0.0 6 955 10 0 1 241 391 0.0 6 375 15 1 242 429 0.0 6 416 13 243 339 0.0 6 329 10 244 292 0.0 6 282 10 245 371 0.0 6 359 11 1 246 314 0.0 6 304 10 247 519 0.0 6 508 11 248 220 0.0 6 216 4 249 401 0.0 6 388 12 0 1 250 496 0.0 6 484 12 251 433 0.0 6 415 18 252 1091 0.0 6 1068 21 1 0 0 1 253 419 0.0 6 408 10 1 254 513 0.0 6 496 17 255 634 0.0 6 612 20 1 1 256 606 0.0 6 595 11 257 1048 0.0 6 1024 22 2 258 671 0.0 6 644 27 259 1918 0.0 6 1875 40 3 260 954 0.0 6 920 32 2 261 1345 0.0 6 1314 30 1 262 718 0.0 6 682 36 263 1502 0.0 6 1470 32 264 678 0.0 6 662 16 265 775 0.0 6 754 20 1 266 634 0.0 6 619 14 1 267 1046 0.0 6 1021 25 268 517 0.0 6 487 30 269 937 0.0 6 909 26 1 1 270 575 0.0 6 551 23 1 271 1116 0.0 6 1093 22 1 272 1055 0.0 6 1025 30 273 519 0.0 6 505 13 1 274 562 0.0 6 542 19 1 275 856 0.0 6 832 24 276 2220 0.0 6 2174 44 1 0 0 0 1 277 2288 0.0 6 2241 45 2 278 2556 0.0 6 2503 52 0 0 0 1 279 3749 0.0 6 3684 62 2 0 1 280 6452 0.0 6 6226 217 8 0 0 1 281 27275 0.0 6 26847 421 6 1 282 16 0.0 6 16 283 3 0.0 6 2 0 1 284 3 0.0 6 2 0 1 285 2 0.0 6 2 289 1 0.0 6 1 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 28 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 60 1 0.0 6 1 73 1 0.0 6 1 75 1 0.0 6 1 81 1 0.0 6 1 82 2 0.0 6 2 88 1 0.0 6 1 89 1 0.0 6 1 90 1 0.0 6 1 91 2 0.0 6 2 92 3 0.0 6 3 96 1 0.0 6 1 98 1 0.0 6 1 99 1 0.0 6 1 101 1 0.0 6 1 104 1 0.0 6 1 110 1 0.0 6 1 129 1 0.0 6 1 141 1 0.0 6 1 142 1 0.0 6 1 158 1 0.0 6 1 180 2 0.0 6 2 193 1 0.0 6 1 221 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 86400 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1351.2 0 1 6 1 21.1 1 0 0 1 7 3 5.3 2 3 12 4 0.0 3 4 13 10 0.0 3 5 5 14 7 0.0 4 7 15 1 0.0 4 1 16 5 0.0 4 5 17 13 0.0 5 11 2 18 4 0.0 5 3 1 19 26 0.0 5 23 3 20 68 0.0 6 64 3 1 21 55 0.0 6 52 3 22 46 0.0 6 46 23 26 0.0 6 25 0 1 24 20 0.0 6 19 1 25 16 0.0 6 16 26 17 0.0 6 17 27 6 0.0 6 6 28 18 0.0 6 15 2 1 29 10 0.0 6 9 1 30 14 0.0 6 11 2 1 31 5 0.0 6 4 1 32 10 0.0 6 9 1 33 17 0.0 6 14 2 1 34 16 0.0 6 16 35 5 0.0 6 4 1 36 5 0.0 6 5 37 11 0.0 6 9 2 38 15 0.0 6 14 1 39 8 0.0 6 7 1 40 6 0.0 6 5 1 41 3 0.0 6 3 42 11 0.0 6 9 2 43 10 0.0 6 7 3 44 14 0.0 6 14 45 4 0.0 6 4 46 20 0.0 6 20 47 18 0.0 6 16 1 1 48 9 0.0 6 9 49 23 0.0 6 20 2 0 0 0 1 50 25 0.0 6 20 5 51 20 0.0 6 19 1 52 26 0.0 6 23 2 1 53 12 0.0 6 12 54 8 0.0 6 5 2 1 55 33 0.0 6 32 1 56 34 0.0 6 32 2 57 25 0.0 6 23 1 0 0 1 58 21 0.0 6 17 3 0 0 1 59 25 0.0 6 24 1 60 22 0.0 6 19 3 61 13 0.0 6 12 0 1 62 16 0.0 6 14 2 63 9 0.0 6 9 64 10 0.0 6 7 3 65 12 0.0 6 11 1 66 19 0.0 6 19 67 8 0.0 6 8 68 23 0.0 6 21 2 69 29 0.0 6 26 1 2 70 150 0.0 6 145 3 1 0 1 71 30 0.0 6 24 5 0 1 72 90 0.0 6 81 9 73 21 0.0 6 20 0 0 0 1 74 49 0.0 6 48 1 75 22 0.0 6 21 0 0 0 0 1 76 20 0.0 6 19 1 77 16 0.0 6 15 1 78 28 0.0 6 25 3 79 12 0.0 6 11 1 80 21 0.0 6 21 81 15 0.0 6 13 2 82 18 0.0 6 15 3 83 51 0.0 6 41 7 0 0 3 84 59 0.0 6 55 4 85 57 0.0 6 54 2 0 1 86 45 0.0 6 41 4 87 50 0.0 6 48 2 88 24 0.0 6 22 2 89 33 0.0 6 30 3 90 47 0.0 6 45 2 91 31 0.0 6 27 4 92 36 0.0 6 34 1 0 1 93 37 0.0 6 31 6 94 87 0.0 6 84 3 95 23 0.0 6 23 96 46 0.0 6 42 4 97 41 0.0 6 37 2 1 0 1 98 27 0.0 6 25 2 99 63 0.0 6 57 5 0 0 1 100 47 0.0 6 40 7 101 53 0.0 6 51 2 102 85 0.0 6 79 6 103 86 0.0 6 72 12 2 104 73 0.0 6 70 3 105 73 0.0 6 72 0 0 0 1 106 48 0.0 6 46 2 107 86 0.0 6 83 3 108 57 0.0 6 53 3 1 109 35 0.0 6 32 3 110 52 0.0 6 45 6 1 111 38 0.0 6 37 1 112 60 0.0 6 56 4 113 36 0.0 6 34 2 114 44 0.0 6 44 115 62 0.0 6 59 3 116 48 0.0 6 48 117 41 0.0 6 39 1 0 0 1 118 41 0.0 6 35 6 119 75 0.0 6 67 7 0 0 1 120 105 0.0 6 97 8 121 67 0.0 6 57 9 0 0 0 1 122 82 0.0 6 75 6 0 0 1 123 102 0.0 6 94 8 124 133 0.0 6 127 6 125 77 0.0 6 70 5 1 0 1 126 45 0.0 6 42 2 0 0 1 127 70 0.0 6 66 4 128 93 0.0 6 86 6 1 129 78 0.0 6 74 3 0 0 1 130 59 0.0 6 55 4 131 100 0.0 6 93 7 132 200 0.0 6 195 5 133 121 0.0 6 116 5 134 179 0.0 6 169 10 135 295 0.0 6 274 17 1 0 2 1 136 165 0.0 6 150 10 3 1 1 137 250 0.0 6 236 14 138 165 0.0 6 157 6 1 0 1 139 148 0.0 6 140 6 1 0 1 140 179 0.0 6 171 7 0 0 1 141 284 0.0 6 275 8 1 142 295 0.0 6 285 10 143 264 0.0 6 247 15 1 0 1 144 439 0.0 6 420 17 0 0 1 1 145 242 0.0 6 233 7 2 146 250 0.0 6 245 4 0 0 1 147 128 0.0 6 125 2 0 0 1 148 193 0.0 6 184 8 0 1 149 345 0.0 6 333 10 1 1 150 512 0.0 6 501 10 0 0 1 151 355 0.0 6 342 10 1 1 0 1 152 623 0.0 6 599 22 0 1 1 153 593 0.0 6 585 8 154 440 0.0 6 426 12 1 0 1 155 473 0.0 6 456 13 0 0 4 156 450 0.0 6 433 17 157 494 0.0 6 481 13 158 596 0.0 6 585 8 2 0 1 159 248 0.0 6 237 10 1 160 641 0.0 6 629 10 0 0 2 161 442 0.0 6 429 11 1 1 162 316 0.0 6 306 10 163 507 0.0 6 482 23 1 0 0 1 164 474 0.0 6 463 11 165 655 0.0 6 637 17 0 1 166 763 0.0 6 741 20 1 0 1 167 363 0.0 6 354 7 2 168 845 0.0 6 826 17 0 1 1 169 324 0.0 6 312 12 170 324 0.0 6 307 16 0 0 0 1 171 622 0.0 6 605 17 172 264 0.0 6 257 7 173 361 0.0 6 344 14 1 1 1 174 498 0.0 6 482 15 0 0 1 175 954 0.0 6 930 23 1 176 571 0.0 6 555 16 177 971 0.0 6 932 34 2 1 2 178 525 0.0 6 502 22 0 0 1 179 749 0.0 6 715 32 1 0 0 1 180 803 0.0 6 777 25 0 1 181 1167 0.0 6 1140 25 1 0 0 0 1 182 461 0.0 6 435 21 4 1 183 854 0.0 6 833 19 0 0 2 184 263 0.0 6 248 10 3 0 2 185 277 0.0 6 263 11 1 1 1 186 1113 0.0 6 1079 32 2 187 1336 0.0 6 1291 44 1 188 1835 0.0 6 1770 62 1 0 2 189 1583 0.0 6 1535 48 190 2191 0.0 6 2126 61 1 1 2 191 1907 0.0 6 1856 49 1 0 1 192 1432 0.0 6 1383 48 1 193 1180 0.0 6 1147 33 194 1490 0.0 6 1443 39 7 0 1 195 1503 0.0 6 1452 48 3 196 3938 0.0 6 3830 105 1 1 1 197 806 0.0 6 778 27 0 0 1 198 2115 0.0 6 2057 54 1 2 1 199 3377 0.0 6 3320 56 1 200 1374 0.0 6 1338 29 5 0 2 201 1984 0.0 6 1935 45 2 0 2 202 1229 0.0 6 1197 32 203 925 0.0 6 895 29 1 204 1101 0.0 6 1071 30 205 847 0.0 6 817 27 0 1 2 206 745 0.0 6 724 21 207 643 0.0 6 628 13 1 1 208 1250 0.0 6 1222 26 2 209 398 0.0 6 382 15 1 210 657 0.0 6 645 11 1 211 1145 0.0 6 1112 29 3 0 0 1 212 880 0.0 6 858 21 0 1 213 205 0.0 6 202 3 214 736 0.0 6 721 14 0 1 215 779 0.0 6 759 20 216 1111 0.0 6 1084 25 2 217 1013 0.0 6 991 20 0 1 1 218 468 0.0 6 456 12 219 459 0.0 6 437 22 220 386 0.0 6 369 17 221 515 0.0 6 501 13 1 222 904 0.0 6 884 20 223 741 0.0 6 726 15 224 215 0.0 6 209 5 0 0 0 1 225 389 0.0 6 375 13 1 226 555 0.0 6 544 11 227 972 0.0 6 950 20 1 1 228 636 0.0 6 613 22 0 1 229 714 0.0 6 688 24 1 1 230 1100 0.0 6 1075 25 231 618 0.0 6 596 21 1 232 1517 0.0 6 1480 36 0 0 1 233 280 0.0 6 271 9 234 443 0.0 6 425 18 235 378 0.0 6 363 15 236 243 0.0 6 235 7 0 1 237 299 0.0 6 290 9 238 188 0.0 6 185 3 239 53 0.0 6 51 2 240 110 0.0 6 106 4 241 261 0.0 6 259 2 242 231 0.0 6 226 5 243 101 0.0 6 98 3 244 257 0.0 6 250 7 245 347 0.0 6 339 8 246 158 0.0 6 152 6 247 160 0.0 6 154 5 1 248 113 0.0 6 112 1 249 183 0.0 6 177 6 250 126 0.0 6 124 2 251 332 0.0 6 322 9 1 252 134 0.0 6 130 3 1 253 54 0.0 6 52 2 254 321 0.0 6 312 9 255 50 0.0 6 47 3 256 46 0.0 6 44 1 1 257 7 0.0 6 7 258 2 0.0 6 2 260 2 0.0 6 2 265 1 0.0 6 1 266 1 0.0 6 1 269 1 0.0 6 1 270 1 0.0 6 1 277 1 0.0 6 1 278 1 0.0 6 1 279 1 0.0 6 1 280 11 0.0 6 11 281 27 0.0 6 26 1 282 168 0.0 6 161 7 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 41 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 73 1 0.0 6 1 76 1 0.0 6 1 77 1 0.0 6 1 79 1 0.0 6 1 80 3 0.0 6 3 81 1 0.0 6 1 82 1 0.0 6 1 83 1 0.0 6 1 84 2 0.0 6 2 90 1 0.0 6 1 95 1 0.0 6 1 97 1 0.0 6 1 98 1 0.0 6 1 99 1 0.0 6 1 100 3 0.0 6 3 103 1 0.0 6 1 104 1 0.0 6 1 107 1 0.0 6 1 110 1 0.0 6 1 117 1 0.0 6 1 125 1 0.0 6 1 128 2 0.0 6 2 129 1 0.0 6 1 132 1 0.0 6 1 134 1 0.0 6 1 140 1 0.0 6 1 141 3 0.0 6 3 156 1 0.0 6 1 179 2 0.0 6 2 192 1 0.0 6 1 193 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_10_202_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_10_429_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_10_202_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_10_429_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.50 s (71 µs/read; 0.84 M reads/minute). === Summary === Total read pairs processed: 49,042 Read 1 with adapter: 49,038 (100.0%) Read 2 with adapter: 49,018 (100.0%) == Read fate breakdown == Pairs that were too short: 19 (0.0%) Pairs discarded as untrimmed: 28 (0.1%) Pairs written (passing filters): 48,995 (99.9%) Total basepairs processed: 21,491,575 bp Read 1: 12,172,756 bp Read 2: 9,318,819 bp Total written (filtered): 3,109,501 bp (14.5%) Read 1: 1,523,678 bp Read 2: 1,585,823 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 47947 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 766.3 0 1 2 7 1 3.0 2 0 0 1 8 1 0.7 2 1 9 2 0.2 2 2 11 2 0.0 3 2 13 1 0.0 3 1 18 2 0.0 5 2 19 2 0.0 5 1 0 0 1 20 60 0.0 6 59 1 21 30 0.0 6 28 2 22 37 0.0 6 37 23 21 0.0 6 19 2 24 16 0.0 6 15 1 25 11 0.0 6 11 26 16 0.0 6 16 27 9 0.0 6 9 28 3 0.0 6 3 29 9 0.0 6 9 30 2 0.0 6 2 31 3 0.0 6 3 32 10 0.0 6 10 33 4 0.0 6 4 34 3 0.0 6 3 35 1 0.0 6 1 36 2 0.0 6 2 37 1 0.0 6 1 38 3 0.0 6 3 39 4 0.0 6 4 40 5 0.0 6 5 41 2 0.0 6 2 42 1 0.0 6 1 43 6 0.0 6 6 44 8 0.0 6 8 45 3 0.0 6 2 1 46 7 0.0 6 5 2 47 3 0.0 6 3 48 2 0.0 6 2 49 2 0.0 6 2 50 1 0.0 6 1 51 6 0.0 6 6 52 4 0.0 6 4 53 1 0.0 6 1 54 3 0.0 6 3 55 4 0.0 6 4 56 4 0.0 6 4 57 3 0.0 6 3 58 5 0.0 6 5 60 5 0.0 6 5 61 1 0.0 6 1 62 6 0.0 6 6 63 5 0.0 6 5 64 2 0.0 6 2 65 4 0.0 6 4 66 5 0.0 6 5 67 7 0.0 6 7 68 4 0.0 6 4 69 3 0.0 6 3 70 6 0.0 6 6 71 5 0.0 6 5 72 4 0.0 6 4 73 8 0.0 6 8 74 5 0.0 6 5 75 7 0.0 6 6 1 76 6 0.0 6 6 77 8 0.0 6 8 78 6 0.0 6 6 79 4 0.0 6 4 80 6 0.0 6 5 0 1 81 7 0.0 6 7 82 7 0.0 6 5 2 83 9 0.0 6 9 84 6 0.0 6 5 0 1 85 4 0.0 6 3 1 86 12 0.0 6 12 87 8 0.0 6 7 1 88 6 0.0 6 6 89 3 0.0 6 3 90 10 0.0 6 10 91 9 0.0 6 9 92 10 0.0 6 9 1 93 2 0.0 6 2 94 9 0.0 6 8 1 95 7 0.0 6 7 96 3 0.0 6 3 97 6 0.0 6 6 98 8 0.0 6 8 99 9 0.0 6 9 100 9 0.0 6 7 1 0 0 1 101 4 0.0 6 4 102 4 0.0 6 4 103 4 0.0 6 4 104 8 0.0 6 8 105 15 0.0 6 13 1 1 106 8 0.0 6 8 107 9 0.0 6 9 108 7 0.0 6 7 109 13 0.0 6 12 0 0 0 1 110 6 0.0 6 6 111 9 0.0 6 9 112 20 0.0 6 19 1 113 8 0.0 6 8 114 14 0.0 6 14 115 7 0.0 6 7 116 7 0.0 6 7 117 17 0.0 6 16 1 118 33 0.0 6 32 1 119 16 0.0 6 16 120 13 0.0 6 13 121 19 0.0 6 18 1 122 19 0.0 6 19 123 9 0.0 6 9 124 18 0.0 6 18 125 13 0.0 6 13 126 9 0.0 6 9 127 13 0.0 6 13 128 11 0.0 6 10 1 129 16 0.0 6 16 130 16 0.0 6 16 131 24 0.0 6 24 132 20 0.0 6 18 2 133 23 0.0 6 22 1 134 16 0.0 6 16 135 30 0.0 6 29 1 136 17 0.0 6 17 137 22 0.0 6 22 138 23 0.0 6 23 139 22 0.0 6 22 140 19 0.0 6 19 141 27 0.0 6 27 142 43 0.0 6 43 143 75 0.0 6 73 2 144 191 0.0 6 190 1 145 259 0.0 6 259 146 169 0.0 6 168 1 147 153 0.0 6 150 3 148 248 0.0 6 248 149 224 0.0 6 223 1 150 205 0.0 6 201 4 151 154 0.0 6 154 152 216 0.0 6 216 153 229 0.0 6 228 1 154 237 0.0 6 237 155 156 0.0 6 155 1 156 160 0.0 6 158 2 157 177 0.0 6 177 158 203 0.0 6 203 159 129 0.0 6 129 160 196 0.0 6 195 1 161 173 0.0 6 172 1 162 287 0.0 6 286 1 163 174 0.0 6 172 1 1 164 133 0.0 6 133 165 169 0.0 6 168 1 166 196 0.0 6 196 167 138 0.0 6 138 168 131 0.0 6 131 169 195 0.0 6 195 170 179 0.0 6 178 1 171 215 0.0 6 214 1 172 144 0.0 6 144 173 145 0.0 6 143 1 0 0 0 0 1 174 149 0.0 6 148 1 175 176 0.0 6 176 176 209 0.0 6 208 1 177 319 0.0 6 319 178 170 0.0 6 169 1 179 153 0.0 6 153 180 215 0.0 6 214 1 181 460 0.0 6 458 2 182 236 0.0 6 235 0 1 183 175 0.0 6 174 1 184 264 0.0 6 264 185 242 0.0 6 237 5 186 230 0.0 6 230 187 313 0.0 6 311 2 188 392 0.0 6 391 1 189 208 0.0 6 207 1 190 200 0.0 6 197 3 191 283 0.0 6 283 192 280 0.0 6 280 193 346 0.0 6 343 3 194 312 0.0 6 309 3 195 326 0.0 6 324 1 0 0 0 1 196 480 0.0 6 478 1 0 1 197 411 0.0 6 406 4 0 1 198 452 0.0 6 449 3 199 579 0.0 6 573 5 0 0 0 1 200 562 0.0 6 560 2 201 379 0.0 6 371 7 1 202 345 0.0 6 341 4 203 395 0.0 6 391 4 204 376 0.0 6 375 1 205 631 0.0 6 627 3 1 206 522 0.0 6 519 3 207 437 0.0 6 432 5 208 531 0.0 6 528 3 209 410 0.0 6 410 210 513 0.0 6 509 4 211 403 0.0 6 398 5 212 415 0.0 6 412 2 1 213 523 0.0 6 519 3 0 0 1 214 570 0.0 6 569 0 0 0 1 215 491 0.0 6 490 1 216 647 0.0 6 644 3 217 933 0.0 6 929 3 0 1 218 568 0.0 6 565 3 219 535 0.0 6 531 3 1 220 551 0.0 6 547 4 221 567 0.0 6 562 5 222 501 0.0 6 499 2 223 530 0.0 6 526 3 0 0 1 224 529 0.0 6 526 2 1 225 493 0.0 6 491 1 1 226 525 0.0 6 524 1 227 586 0.0 6 582 3 0 0 1 228 524 0.0 6 521 2 0 1 229 422 0.0 6 418 3 0 1 230 379 0.0 6 374 4 0 0 0 0 1 231 357 0.0 6 356 1 232 472 0.0 6 471 1 233 389 0.0 6 388 1 234 398 0.0 6 398 235 341 0.0 6 341 236 296 0.0 6 293 3 237 270 0.0 6 269 1 238 295 0.0 6 291 4 239 327 0.0 6 324 3 240 320 0.0 6 315 5 241 298 0.0 6 295 3 242 306 0.0 6 304 2 243 283 0.0 6 281 2 244 334 0.0 6 329 5 245 277 0.0 6 272 5 246 251 0.0 6 248 3 247 259 0.0 6 256 3 248 195 0.0 6 193 2 249 196 0.0 6 195 1 250 232 0.0 6 228 3 1 251 191 0.0 6 188 3 252 188 0.0 6 187 1 253 174 0.0 6 170 4 254 168 0.0 6 165 3 255 169 0.0 6 169 256 141 0.0 6 140 0 0 1 257 156 0.0 6 152 4 258 152 0.0 6 149 2 0 1 259 155 0.0 6 151 3 1 260 157 0.0 6 154 3 261 153 0.0 6 151 2 262 127 0.0 6 125 2 263 125 0.0 6 123 2 264 133 0.0 6 130 3 265 111 0.0 6 110 1 266 89 0.0 6 88 1 267 99 0.0 6 98 1 268 75 0.0 6 71 4 269 69 0.0 6 68 1 270 76 0.0 6 75 1 271 98 0.0 6 94 4 272 136 0.0 6 133 3 273 82 0.0 6 79 3 274 97 0.0 6 95 1 1 275 79 0.0 6 76 3 276 149 0.0 6 142 7 277 274 0.0 6 269 5 278 148 0.0 6 146 1 1 279 276 0.0 6 270 6 280 634 0.0 6 611 22 1 281 6895 0.0 6 6785 107 1 2 282 7 0.0 6 5 0 1 1 283 2 0.0 6 1 0 0 0 1 285 1 0.0 6 1 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1091 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 24 1 0.0 6 0 0 0 0 0 0 1 60 1 0.0 6 1 61 116 0.0 6 113 3 62 1 0.0 6 1 65 1 0.0 6 0 0 0 0 0 1 69 1 0.0 6 1 70 2 0.0 6 2 71 6 0.0 6 6 72 6 0.0 6 6 73 5 0.0 6 5 74 3 0.0 6 3 75 6 0.0 6 6 76 2 0.0 6 2 77 4 0.0 6 4 78 4 0.0 6 4 79 6 0.0 6 6 80 4 0.0 6 4 81 5 0.0 6 5 82 4 0.0 6 4 83 3 0.0 6 3 84 3 0.0 6 3 85 5 0.0 6 5 86 3 0.0 6 3 87 6 0.0 6 6 88 2 0.0 6 2 89 8 0.0 6 8 90 3 0.0 6 3 91 3 0.0 6 3 92 3 0.0 6 3 93 3 0.0 6 3 94 3 0.0 6 3 95 5 0.0 6 5 96 5 0.0 6 5 97 4 0.0 6 4 98 2 0.0 6 2 99 2 0.0 6 2 100 3 0.0 6 3 101 1 0.0 6 1 102 3 0.0 6 3 103 7 0.0 6 7 104 7 0.0 6 7 105 2 0.0 6 2 106 4 0.0 6 4 107 5 0.0 6 5 108 16 0.0 6 16 109 1 0.0 6 1 110 9 0.0 6 9 111 4 0.0 6 4 112 2 0.0 6 2 113 5 0.0 6 5 114 10 0.0 6 10 115 10 0.0 6 10 116 6 0.0 6 6 117 4 0.0 6 4 118 8 0.0 6 8 119 5 0.0 6 5 120 5 0.0 6 5 121 2 0.0 6 2 122 7 0.0 6 7 123 8 0.0 6 8 124 1 0.0 6 1 125 6 0.0 6 6 126 10 0.0 6 10 128 4 0.0 6 4 129 2 0.0 6 2 130 3 0.0 6 3 131 8 0.0 6 8 132 10 0.0 6 10 133 7 0.0 6 7 134 5 0.0 6 5 135 4 0.0 6 4 136 2 0.0 6 2 137 7 0.0 6 7 139 4 0.0 6 4 140 10 0.0 6 10 141 3 0.0 6 3 142 7 0.0 6 7 143 10 0.0 6 10 144 5 0.0 6 5 145 1 0.0 6 1 146 7 0.0 6 7 147 3 0.0 6 3 148 1 0.0 6 1 149 5 0.0 6 5 150 5 0.0 6 5 151 13 0.0 6 13 152 6 0.0 6 6 153 11 0.0 6 11 154 7 0.0 6 7 155 5 0.0 6 5 156 8 0.0 6 8 157 9 0.0 6 9 158 5 0.0 6 5 159 10 0.0 6 10 160 7 0.0 6 7 161 9 0.0 6 9 162 6 0.0 6 6 163 6 0.0 6 6 164 8 0.0 6 8 165 8 0.0 6 8 166 9 0.0 6 9 167 3 0.0 6 3 168 6 0.0 6 6 169 5 0.0 6 5 170 3 0.0 6 3 171 11 0.0 6 11 172 9 0.0 6 9 173 13 0.0 6 13 174 5 0.0 6 5 175 1 0.0 6 1 176 12 0.0 6 12 177 16 0.0 6 16 178 9 0.0 6 9 179 3 0.0 6 3 180 2 0.0 6 2 181 4 0.0 6 4 182 6 0.0 6 6 183 17 0.0 6 17 184 7 0.0 6 7 185 8 0.0 6 8 186 15 0.0 6 14 1 187 7 0.0 6 7 188 15 0.0 6 15 189 7 0.0 6 7 190 8 0.0 6 8 191 8 0.0 6 8 192 10 0.0 6 10 193 8 0.0 6 8 194 15 0.0 6 15 195 5 0.0 6 5 196 3 0.0 6 3 197 7 0.0 6 7 198 2 0.0 6 2 199 5 0.0 6 5 200 5 0.0 6 5 201 8 0.0 6 8 202 7 0.0 6 7 203 4 0.0 6 4 204 4 0.0 6 4 205 4 0.0 6 4 206 2 0.0 6 2 207 6 0.0 6 6 208 3 0.0 6 3 209 4 0.0 6 4 210 4 0.0 6 4 211 3 0.0 6 3 212 2 0.0 6 2 213 1 0.0 6 1 214 4 0.0 6 4 215 1 0.0 6 1 216 2 0.0 6 2 217 2 0.0 6 2 218 4 0.0 6 4 219 6 0.0 6 6 220 6 0.0 6 6 221 5 0.0 6 5 222 6 0.0 6 6 223 1 0.0 6 1 224 1 0.0 6 1 225 4 0.0 6 4 226 4 0.0 6 4 227 2 0.0 6 2 228 4 0.0 6 4 229 3 0.0 6 3 230 7 0.0 6 7 231 2 0.0 6 2 232 3 0.0 6 3 233 1 0.0 6 1 234 1 0.0 6 1 235 1 0.0 6 1 236 6 0.0 6 6 237 2 0.0 6 2 239 1 0.0 6 1 240 1 0.0 6 1 241 2 0.0 6 2 243 6 0.0 6 6 244 3 0.0 6 3 245 2 0.0 6 2 246 3 0.0 6 3 247 5 0.0 6 5 248 1 0.0 6 1 249 3 0.0 6 3 250 3 0.0 6 3 251 2 0.0 6 2 252 3 0.0 6 3 254 1 0.0 6 1 255 2 0.0 6 2 258 3 0.0 6 3 259 3 0.0 6 3 260 2 0.0 6 2 261 4 0.0 6 4 265 1 0.0 6 1 274 1 0.0 6 1 275 2 0.0 6 2 276 1 0.0 6 1 277 1 0.0 6 1 278 1 0.0 6 1 280 1 0.0 6 1 282 2 0.0 6 2 285 2 0.0 6 2 286 1 0.0 6 1 287 1 0.0 6 1 288 1 0.0 6 1 294 1 0.0 6 1 297 1 0.0 6 1 300 1 0.0 6 1 308 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 48183 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 766.3 0 0 3 5 1 47.9 1 1 8 1 0.7 2 1 9 2 0.2 2 2 10 1 0.0 3 1 11 2 0.0 3 2 12 1 0.0 3 1 13 3 0.0 3 3 14 3 0.0 4 3 16 12 0.0 4 11 1 17 19 0.0 5 14 5 18 18 0.0 5 18 19 13 0.0 5 10 2 1 20 63 0.0 6 60 2 1 21 38 0.0 6 36 2 22 15 0.0 6 14 1 23 15 0.0 6 14 1 24 23 0.0 6 23 25 29 0.0 6 27 2 26 23 0.0 6 21 2 27 13 0.0 6 11 2 28 9 0.0 6 9 29 13 0.0 6 10 1 0 0 0 1 1 30 13 0.0 6 13 31 15 0.0 6 15 32 13 0.0 6 12 1 33 11 0.0 6 10 1 34 27 0.0 6 27 35 12 0.0 6 12 36 11 0.0 6 10 1 37 18 0.0 6 16 2 38 13 0.0 6 12 1 39 14 0.0 6 11 2 1 40 10 0.0 6 9 1 41 8 0.0 6 7 1 42 4 0.0 6 3 1 43 18 0.0 6 15 2 1 44 11 0.0 6 11 45 13 0.0 6 13 46 9 0.0 6 9 47 13 0.0 6 10 2 0 0 0 0 1 48 19 0.0 6 15 4 49 12 0.0 6 10 2 50 17 0.0 6 14 3 51 14 0.0 6 14 52 16 0.0 6 15 1 53 19 0.0 6 19 54 16 0.0 6 16 55 16 0.0 6 15 1 56 18 0.0 6 16 2 57 22 0.0 6 21 1 58 16 0.0 6 15 1 59 16 0.0 6 16 60 13 0.0 6 11 2 61 20 0.0 6 20 62 24 0.0 6 21 3 63 29 0.0 6 29 64 16 0.0 6 15 1 65 17 0.0 6 16 0 0 0 1 66 28 0.0 6 27 1 67 100 0.0 6 94 5 1 68 33 0.0 6 31 2 69 47 0.0 6 45 2 70 29 0.0 6 27 2 71 30 0.0 6 30 72 34 0.0 6 29 5 73 28 0.0 6 27 1 74 23 0.0 6 22 1 75 36 0.0 6 34 1 1 76 32 0.0 6 31 1 77 33 0.0 6 31 2 78 29 0.0 6 27 2 79 31 0.0 6 31 80 34 0.0 6 31 2 1 81 53 0.0 6 51 2 82 55 0.0 6 53 2 83 49 0.0 6 43 5 0 0 1 84 31 0.0 6 31 85 32 0.0 6 30 0 1 0 0 1 86 39 0.0 6 36 3 87 35 0.0 6 34 1 88 32 0.0 6 31 0 0 1 89 49 0.0 6 47 2 90 49 0.0 6 44 4 1 91 63 0.0 6 62 1 92 50 0.0 6 49 1 93 74 0.0 6 71 3 94 68 0.0 6 64 4 95 66 0.0 6 64 2 96 60 0.0 6 58 2 97 58 0.0 6 56 2 98 61 0.0 6 58 2 0 0 1 99 59 0.0 6 57 2 100 70 0.0 6 67 2 1 101 75 0.0 6 68 4 0 1 1 1 102 69 0.0 6 64 3 0 0 1 0 1 103 107 0.0 6 105 2 104 135 0.0 6 129 4 0 1 0 1 105 92 0.0 6 91 1 106 72 0.0 6 70 2 107 81 0.0 6 79 2 108 80 0.0 6 77 1 0 0 1 0 1 109 100 0.0 6 95 4 0 1 110 105 0.0 6 97 8 111 108 0.0 6 102 6 112 152 0.0 6 145 6 0 0 1 113 107 0.0 6 104 2 0 1 114 119 0.0 6 111 7 1 115 121 0.0 6 118 3 116 135 0.0 6 130 4 0 0 0 0 1 117 143 0.0 6 138 5 118 162 0.0 6 154 7 0 1 119 157 0.0 6 149 5 0 0 1 1 1 120 144 0.0 6 138 6 121 143 0.0 6 141 2 122 165 0.0 6 161 3 0 0 0 0 1 123 148 0.0 6 143 5 124 168 0.0 6 162 5 0 0 1 125 220 0.0 6 209 9 0 1 0 1 126 212 0.0 6 207 3 0 1 0 0 1 127 236 0.0 6 224 9 0 0 1 1 1 128 236 0.0 6 234 2 129 289 0.0 6 284 5 130 246 0.0 6 239 7 131 286 0.0 6 279 5 0 1 0 0 1 132 290 0.0 6 283 6 1 133 343 0.0 6 330 9 2 2 134 340 0.0 6 329 10 0 0 0 0 1 135 367 0.0 6 359 8 136 328 0.0 6 322 5 0 0 0 0 1 137 352 0.0 6 347 4 0 0 0 0 1 138 398 0.0 6 389 9 139 455 0.0 6 449 5 0 1 140 584 0.0 6 574 8 1 1 141 488 0.0 6 469 16 1 1 0 0 1 142 584 0.0 6 577 6 0 0 0 0 1 143 656 0.0 6 650 5 1 144 640 0.0 6 628 11 0 0 0 0 1 145 647 0.0 6 634 10 0 1 0 2 146 622 0.0 6 610 12 147 732 0.0 6 717 12 0 3 148 785 0.0 6 774 11 149 989 0.0 6 973 13 0 1 1 1 150 1027 0.0 6 1013 12 2 151 852 0.0 6 839 12 1 152 974 0.0 6 954 18 1 1 153 1012 0.0 6 990 19 0 1 2 154 730 0.0 6 725 5 155 825 0.0 6 809 14 1 0 0 0 1 156 732 0.0 6 722 9 1 157 841 0.0 6 826 14 0 0 1 158 764 0.0 6 755 7 2 159 716 0.0 6 708 7 0 0 1 160 670 0.0 6 661 7 0 1 0 0 1 161 616 0.0 6 604 12 162 553 0.0 6 547 5 0 0 0 0 1 163 616 0.0 6 607 8 0 0 1 164 561 0.0 6 550 9 0 2 165 552 0.0 6 544 7 0 1 166 685 0.0 6 678 5 1 1 167 559 0.0 6 550 7 0 0 1 0 1 168 681 0.0 6 671 9 1 169 525 0.0 6 518 7 170 515 0.0 6 510 3 2 171 687 0.0 6 681 5 1 172 611 0.0 6 602 7 0 0 0 1 1 173 573 0.0 6 566 7 174 588 0.0 6 577 9 0 0 2 175 567 0.0 6 551 14 1 0 0 1 176 607 0.0 6 598 9 177 524 0.0 6 514 8 0 1 1 178 465 0.0 6 455 10 179 370 0.0 6 366 4 180 328 0.0 6 321 6 0 0 1 181 325 0.0 6 315 10 182 307 0.0 6 300 7 183 469 0.0 6 459 10 184 551 0.0 6 546 5 185 504 0.0 6 496 7 0 0 0 0 1 186 496 0.0 6 487 7 0 0 1 1 187 433 0.0 6 426 7 188 409 0.0 6 403 6 189 392 0.0 6 382 10 190 396 0.0 6 384 11 1 191 438 0.0 6 434 4 192 250 0.0 6 247 2 0 1 193 227 0.0 6 224 1 0 0 0 0 2 194 313 0.0 6 309 3 0 0 0 0 1 195 343 0.0 6 335 7 0 1 196 526 0.0 6 512 13 1 197 253 0.0 6 242 10 0 0 1 198 370 0.0 6 359 11 199 440 0.0 6 430 9 0 0 0 1 200 226 0.0 6 218 7 0 0 1 201 254 0.0 6 250 4 202 185 0.0 6 177 8 203 152 0.0 6 147 4 1 204 193 0.0 6 187 5 0 0 1 205 97 0.0 6 93 4 206 84 0.0 6 79 5 207 82 0.0 6 79 2 0 0 0 0 1 208 98 0.0 6 96 2 209 91 0.0 6 88 3 210 90 0.0 6 89 1 211 74 0.0 6 72 2 212 137 0.0 6 133 4 213 74 0.0 6 72 2 214 193 0.0 6 190 3 215 134 0.0 6 129 5 216 116 0.0 6 112 4 217 100 0.0 6 98 2 218 86 0.0 6 84 2 219 87 0.0 6 86 1 220 33 0.0 6 32 1 221 83 0.0 6 82 1 222 95 0.0 6 91 4 223 112 0.0 6 110 2 224 54 0.0 6 48 5 1 225 59 0.0 6 58 1 226 36 0.0 6 35 0 0 0 0 1 227 66 0.0 6 65 1 228 36 0.0 6 34 2 229 72 0.0 6 71 1 230 37 0.0 6 36 1 231 57 0.0 6 53 4 232 64 0.0 6 62 2 233 32 0.0 6 31 1 234 30 0.0 6 28 1 0 0 1 235 16 0.0 6 16 236 3 0.0 6 3 237 5 0.0 6 5 238 7 0.0 6 7 239 16 0.0 6 15 1 240 18 0.0 6 16 2 241 30 0.0 6 28 2 242 31 0.0 6 31 243 30 0.0 6 28 1 1 244 27 0.0 6 27 245 15 0.0 6 15 246 5 0.0 6 4 1 247 7 0.0 6 7 248 18 0.0 6 18 249 3 0.0 6 3 250 2 0.0 6 2 251 9 0.0 6 9 252 2 0.0 6 2 253 2 0.0 6 2 271 1 0.0 6 1 277 3 0.0 6 3 278 9 0.0 6 9 279 50 0.0 6 48 1 1 280 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 835 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 1 0.0 6 1 72 1 0.0 6 1 73 8 0.0 6 8 74 8 0.0 6 8 75 7 0.0 6 7 76 6 0.0 6 6 77 9 0.0 6 9 78 11 0.0 6 11 79 7 0.0 6 7 80 6 0.0 6 6 81 6 0.0 6 6 82 9 0.0 6 9 83 3 0.0 6 3 84 8 0.0 6 8 85 9 0.0 6 9 86 9 0.0 6 9 87 8 0.0 6 8 88 4 0.0 6 4 89 5 0.0 6 5 90 7 0.0 6 7 91 12 0.0 6 12 92 3 0.0 6 3 93 2 0.0 6 2 94 10 0.0 6 10 95 4 0.0 6 4 96 3 0.0 6 3 97 4 0.0 6 4 98 5 0.0 6 5 99 2 0.0 6 2 100 5 0.0 6 5 101 2 0.0 6 2 102 2 0.0 6 2 104 5 0.0 6 5 105 6 0.0 6 6 106 13 0.0 6 13 107 3 0.0 6 3 108 4 0.0 6 4 109 6 0.0 6 6 110 16 0.0 6 16 111 6 0.0 6 6 112 3 0.0 6 3 113 7 0.0 6 7 114 2 0.0 6 2 115 4 0.0 6 4 116 12 0.0 6 12 117 9 0.0 6 9 118 1 0.0 6 1 119 2 0.0 6 2 120 8 0.0 6 8 121 7 0.0 6 7 122 5 0.0 6 5 123 4 0.0 6 4 124 3 0.0 6 3 125 13 0.0 6 13 126 3 0.0 6 3 127 5 0.0 6 5 128 9 0.0 6 9 129 6 0.0 6 6 130 1 0.0 6 1 131 1 0.0 6 1 132 3 0.0 6 3 133 9 0.0 6 9 134 21 0.0 6 21 135 15 0.0 6 15 136 6 0.0 6 6 137 5 0.0 6 5 138 3 0.0 6 3 139 4 0.0 6 4 140 4 0.0 6 4 141 6 0.0 6 5 1 142 9 0.0 6 9 143 11 0.0 6 11 144 5 0.0 6 5 145 6 0.0 6 6 146 8 0.0 6 8 147 9 0.0 6 9 148 5 0.0 6 5 149 3 0.0 6 3 150 2 0.0 6 2 151 7 0.0 6 7 152 5 0.0 6 5 153 13 0.0 6 12 0 0 1 154 4 0.0 6 4 155 9 0.0 6 9 156 3 0.0 6 3 157 4 0.0 6 4 158 7 0.0 6 7 159 8 0.0 6 8 160 2 0.0 6 2 161 12 0.0 6 12 162 9 0.0 6 9 163 11 0.0 6 10 0 0 0 1 164 2 0.0 6 2 165 9 0.0 6 9 166 5 0.0 6 5 167 7 0.0 6 7 168 3 0.0 6 3 169 2 0.0 6 2 170 10 0.0 6 10 171 2 0.0 6 2 172 3 0.0 6 3 173 11 0.0 6 11 174 8 0.0 6 7 0 0 0 1 175 9 0.0 6 9 176 4 0.0 6 4 177 2 0.0 6 2 178 5 0.0 6 5 179 14 0.0 6 13 0 0 0 1 180 9 0.0 6 9 181 3 0.0 6 3 182 2 0.0 6 2 183 4 0.0 6 3 0 0 0 1 184 13 0.0 6 13 185 20 0.0 6 19 0 0 1 186 16 0.0 6 15 1 187 17 0.0 6 17 188 6 0.0 6 6 189 3 0.0 6 3 190 11 0.0 6 11 191 4 0.0 6 4 192 1 0.0 6 1 193 3 0.0 6 3 194 5 0.0 6 5 195 2 0.0 6 2 196 2 0.0 6 2 197 6 0.0 6 6 198 1 0.0 6 1 199 1 0.0 6 1 200 2 0.0 6 2 201 4 0.0 6 4 203 6 0.0 6 6 204 3 0.0 6 3 208 1 0.0 6 1 210 2 0.0 6 2 212 2 0.0 6 2 213 1 0.0 6 1 214 1 0.0 6 1 215 2 0.0 6 2 216 2 0.0 6 2 219 1 0.0 6 1 221 1 0.0 6 1 225 1 0.0 6 1 228 1 0.0 6 1 231 1 0.0 6 1 232 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_11_203_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_11_430_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_11_203_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_11_430_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.70 s (68 µs/read; 0.88 M reads/minute). === Summary === Total read pairs processed: 54,339 Read 1 with adapter: 54,336 (100.0%) Read 2 with adapter: 54,316 (100.0%) == Read fate breakdown == Pairs that were too short: 4 (0.0%) Pairs discarded as untrimmed: 26 (0.0%) Pairs written (passing filters): 54,309 (99.9%) Total basepairs processed: 26,481,713 bp Read 1: 15,210,145 bp Read 2: 11,271,568 bp Total written (filtered): 3,225,212 bp (12.2%) Read 1: 1,641,742 bp Read 2: 1,583,470 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 54109 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 212.3 1 0 1 8 1 0.8 2 0 0 0 1 9 1 0.2 2 1 15 1 0.0 4 1 20 24 0.0 6 24 21 12 0.0 6 11 1 22 6 0.0 6 6 23 6 0.0 6 5 1 24 3 0.0 6 3 25 5 0.0 6 5 26 4 0.0 6 4 27 2 0.0 6 1 1 28 2 0.0 6 2 29 1 0.0 6 1 30 4 0.0 6 4 31 2 0.0 6 2 32 1 0.0 6 1 33 3 0.0 6 1 2 34 1 0.0 6 1 35 1 0.0 6 1 36 1 0.0 6 1 37 1 0.0 6 1 38 2 0.0 6 2 39 3 0.0 6 2 1 40 5 0.0 6 5 41 4 0.0 6 3 1 43 2 0.0 6 1 1 44 4 0.0 6 3 1 45 6 0.0 6 6 46 1 0.0 6 1 47 2 0.0 6 2 48 1 0.0 6 1 50 1 0.0 6 1 51 1 0.0 6 0 1 52 1 0.0 6 1 53 3 0.0 6 3 54 1 0.0 6 1 55 3 0.0 6 2 1 56 4 0.0 6 3 1 57 1 0.0 6 1 58 2 0.0 6 2 59 1 0.0 6 1 60 2 0.0 6 2 62 2 0.0 6 2 63 2 0.0 6 2 64 2 0.0 6 2 65 1 0.0 6 1 66 1 0.0 6 1 67 1 0.0 6 1 68 3 0.0 6 3 69 1 0.0 6 1 72 1 0.0 6 1 73 2 0.0 6 2 74 4 0.0 6 3 1 75 1 0.0 6 1 76 2 0.0 6 2 77 3 0.0 6 3 78 4 0.0 6 4 79 1 0.0 6 1 80 4 0.0 6 3 1 81 1 0.0 6 1 82 5 0.0 6 5 83 1 0.0 6 1 84 5 0.0 6 5 85 2 0.0 6 2 86 11 0.0 6 10 1 87 7 0.0 6 7 88 2 0.0 6 2 89 1 0.0 6 1 90 2 0.0 6 2 91 5 0.0 6 5 92 2 0.0 6 2 93 1 0.0 6 1 94 2 0.0 6 2 95 4 0.0 6 4 96 4 0.0 6 4 97 6 0.0 6 6 98 6 0.0 6 6 99 6 0.0 6 6 100 3 0.0 6 2 1 101 7 0.0 6 7 102 5 0.0 6 5 103 3 0.0 6 3 104 3 0.0 6 3 105 10 0.0 6 10 106 5 0.0 6 5 107 8 0.0 6 8 108 10 0.0 6 10 109 6 0.0 6 5 1 110 2 0.0 6 2 111 4 0.0 6 4 112 9 0.0 6 7 2 113 9 0.0 6 9 114 4 0.0 6 4 115 8 0.0 6 6 2 116 6 0.0 6 6 117 7 0.0 6 7 118 2 0.0 6 2 119 7 0.0 6 7 120 5 0.0 6 5 121 8 0.0 6 8 122 15 0.0 6 12 3 123 10 0.0 6 8 2 124 9 0.0 6 9 125 13 0.0 6 12 0 1 126 6 0.0 6 6 127 6 0.0 6 6 128 17 0.0 6 17 129 7 0.0 6 6 1 130 1 0.0 6 1 131 7 0.0 6 7 132 6 0.0 6 5 1 133 7 0.0 6 7 134 20 0.0 6 18 2 135 22 0.0 6 21 1 136 13 0.0 6 13 137 25 0.0 6 23 2 138 40 0.0 6 38 2 139 20 0.0 6 20 140 58 0.0 6 57 1 141 103 0.0 6 102 1 142 164 0.0 6 164 143 88 0.0 6 88 144 121 0.0 6 118 3 145 120 0.0 6 119 1 146 133 0.0 6 132 1 147 156 0.0 6 156 148 134 0.0 6 132 1 1 149 147 0.0 6 145 2 150 213 0.0 6 212 1 151 123 0.0 6 121 2 152 87 0.0 6 87 153 104 0.0 6 102 2 154 93 0.0 6 93 155 85 0.0 6 85 156 80 0.0 6 80 157 97 0.0 6 95 2 158 90 0.0 6 90 159 117 0.0 6 115 2 160 103 0.0 6 103 161 82 0.0 6 79 3 162 94 0.0 6 92 2 163 86 0.0 6 82 4 164 101 0.0 6 100 1 165 75 0.0 6 75 166 68 0.0 6 65 3 167 37 0.0 6 36 1 168 64 0.0 6 64 169 79 0.0 6 79 170 86 0.0 6 86 171 80 0.0 6 80 172 75 0.0 6 74 1 173 74 0.0 6 74 174 58 0.0 6 58 175 90 0.0 6 90 176 67 0.0 6 67 177 88 0.0 6 88 178 145 0.0 6 145 179 69 0.0 6 69 180 68 0.0 6 66 2 181 70 0.0 6 67 3 182 82 0.0 6 78 4 183 100 0.0 6 99 1 184 104 0.0 6 104 185 107 0.0 6 106 1 186 134 0.0 6 132 2 187 115 0.0 6 114 1 188 77 0.0 6 77 189 100 0.0 6 99 0 1 190 110 0.0 6 108 2 191 134 0.0 6 133 1 192 123 0.0 6 121 2 193 162 0.0 6 161 1 194 166 0.0 6 165 1 195 145 0.0 6 142 3 196 178 0.0 6 176 2 197 200 0.0 6 197 3 198 194 0.0 6 189 5 199 203 0.0 6 201 2 200 210 0.0 6 205 4 0 0 0 0 1 201 204 0.0 6 199 5 202 202 0.0 6 197 5 203 203 0.0 6 197 5 1 204 178 0.0 6 174 3 0 0 0 1 205 226 0.0 6 225 1 206 181 0.0 6 176 5 207 271 0.0 6 268 3 208 304 0.0 6 298 6 209 226 0.0 6 220 6 210 228 0.0 6 227 1 211 152 0.0 6 148 4 212 125 0.0 6 122 2 1 213 230 0.0 6 226 4 214 199 0.0 6 198 1 215 174 0.0 6 169 5 216 186 0.0 6 181 5 217 341 0.0 6 333 7 0 0 0 0 1 218 214 0.0 6 213 1 219 219 0.0 6 216 3 220 225 0.0 6 220 5 221 206 0.0 6 203 3 222 214 0.0 6 210 4 223 146 0.0 6 142 4 224 150 0.0 6 148 2 225 206 0.0 6 202 4 226 370 0.0 6 363 7 227 395 0.0 6 391 4 228 429 0.0 6 419 10 229 317 0.0 6 309 7 1 230 337 0.0 6 328 8 1 231 241 0.0 6 235 5 0 1 232 365 0.0 6 357 8 233 333 0.0 6 329 3 0 1 234 377 0.0 6 371 6 235 264 0.0 6 262 2 236 197 0.0 6 194 3 237 156 0.0 6 153 3 238 207 0.0 6 203 4 239 200 0.0 6 194 6 240 286 0.0 6 276 10 241 338 0.0 6 333 5 242 360 0.0 6 352 8 243 270 0.0 6 266 4 244 256 0.0 6 249 7 245 345 0.0 6 342 3 246 128 0.0 6 126 1 1 247 206 0.0 6 201 5 248 137 0.0 6 132 5 249 290 0.0 6 283 6 1 250 205 0.0 6 199 6 251 276 0.0 6 270 6 252 276 0.0 6 266 10 253 201 0.0 6 191 10 254 338 0.0 6 330 8 255 214 0.0 6 205 7 0 1 1 256 203 0.0 6 191 12 257 185 0.0 6 175 10 258 200 0.0 6 192 8 259 381 0.0 6 365 16 260 516 0.0 6 502 14 261 159 0.0 6 153 4 2 262 383 0.0 6 365 17 1 263 576 0.0 6 563 12 0 0 1 264 390 0.0 6 380 9 0 0 1 265 501 0.0 6 495 5 0 1 266 277 0.0 6 268 8 1 267 320 0.0 6 309 11 268 267 0.0 6 258 9 269 348 0.0 6 336 11 0 1 270 151 0.0 6 136 13 2 271 492 0.0 6 470 22 272 755 0.0 6 735 20 273 434 0.0 6 427 7 274 498 0.0 6 476 20 1 1 275 654 0.0 6 636 16 2 276 965 0.0 6 938 27 277 758 0.0 6 746 12 278 364 0.0 6 352 11 1 279 653 0.0 6 631 20 1 1 280 387 0.0 6 338 46 3 281 22426 0.0 6 22099 323 3 0 0 0 1 282 5 0.0 6 3 1 1 283 1 0.0 6 1 285 1 0.0 6 1 286 2 0.0 6 2 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 227 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 59 1 0.0 6 0 0 1 61 16 0.0 6 16 68 1 0.0 6 1 70 1 0.0 6 1 71 2 0.0 6 2 72 3 0.0 6 3 73 4 0.0 6 4 74 4 0.0 6 4 75 4 0.0 6 4 76 1 0.0 6 1 77 3 0.0 6 3 78 2 0.0 6 2 79 4 0.0 6 4 80 2 0.0 6 2 81 3 0.0 6 3 82 2 0.0 6 2 83 4 0.0 6 4 84 4 0.0 6 4 85 1 0.0 6 1 87 2 0.0 6 2 88 2 0.0 6 2 89 2 0.0 6 2 90 4 0.0 6 4 91 2 0.0 6 2 92 1 0.0 6 1 97 1 0.0 6 1 98 2 0.0 6 2 99 2 0.0 6 2 101 2 0.0 6 2 102 1 0.0 6 1 103 1 0.0 6 1 105 1 0.0 6 1 106 2 0.0 6 2 107 5 0.0 6 5 108 2 0.0 6 2 109 1 0.0 6 1 110 3 0.0 6 3 113 1 0.0 6 1 114 1 0.0 6 1 115 1 0.0 6 1 116 3 0.0 6 3 117 1 0.0 6 1 121 2 0.0 6 2 123 1 0.0 6 1 124 1 0.0 6 1 125 1 0.0 6 1 126 2 0.0 6 2 127 2 0.0 6 2 128 1 0.0 6 1 129 2 0.0 6 2 130 1 0.0 6 1 132 2 0.0 6 2 135 2 0.0 6 2 136 1 0.0 6 1 137 1 0.0 6 1 138 2 0.0 6 2 139 1 0.0 6 1 140 1 0.0 6 1 142 2 0.0 6 2 144 1 0.0 6 1 145 2 0.0 6 2 146 2 0.0 6 2 148 1 0.0 6 1 149 2 0.0 6 2 150 4 0.0 6 4 151 1 0.0 6 1 152 2 0.0 6 2 153 2 0.0 6 2 156 3 0.0 6 3 157 1 0.0 6 1 159 1 0.0 6 1 161 1 0.0 6 1 162 1 0.0 6 1 165 2 0.0 6 2 167 2 0.0 6 2 168 1 0.0 6 1 169 1 0.0 6 1 170 2 0.0 6 2 171 1 0.0 6 1 173 1 0.0 6 1 175 1 0.0 6 1 176 3 0.0 6 3 177 3 0.0 6 3 178 2 0.0 6 2 179 1 0.0 6 1 182 1 0.0 6 1 186 2 0.0 6 2 187 3 0.0 6 3 189 2 0.0 6 2 192 1 0.0 6 1 193 1 0.0 6 1 194 1 0.0 6 1 196 1 0.0 6 1 197 1 0.0 6 1 199 2 0.0 6 2 200 1 0.0 6 1 201 1 0.0 6 1 203 2 0.0 6 2 206 1 0.0 6 1 207 2 0.0 6 2 208 1 0.0 6 1 209 1 0.0 6 1 210 2 0.0 6 2 212 1 0.0 6 1 215 3 0.0 6 3 217 1 0.0 6 1 223 1 0.0 6 1 224 1 0.0 6 1 226 1 0.0 6 1 227 2 0.0 6 2 228 1 0.0 6 1 229 1 0.0 6 1 230 1 0.0 6 1 236 1 0.0 6 1 238 1 0.0 6 1 239 1 0.0 6 1 240 1 0.0 6 1 244 1 0.0 6 1 253 1 0.0 6 1 259 1 0.0 6 1 265 2 0.0 6 1 1 269 1 0.0 6 1 270 1 0.0 6 1 283 1 0.0 6 1 288 1 0.0 6 1 311 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 54105 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 849.0 0 1 6 1 13.3 1 1 7 1 3.3 2 1 11 2 0.0 3 2 12 3 0.0 3 3 13 5 0.0 3 4 1 14 3 0.0 4 3 15 1 0.0 4 1 16 2 0.0 4 2 17 13 0.0 5 13 18 1 0.0 5 0 1 19 35 0.0 5 33 2 20 60 0.0 6 55 5 21 51 0.0 6 48 3 22 30 0.0 6 30 23 18 0.0 6 18 24 15 0.0 6 15 25 12 0.0 6 11 1 26 10 0.0 6 10 27 12 0.0 6 11 1 28 7 0.0 6 7 29 10 0.0 6 10 30 16 0.0 6 15 1 31 6 0.0 6 6 32 8 0.0 6 8 33 14 0.0 6 14 34 10 0.0 6 9 1 35 9 0.0 6 8 1 36 4 0.0 6 4 37 8 0.0 6 7 1 38 16 0.0 6 13 3 39 10 0.0 6 10 40 3 0.0 6 3 41 7 0.0 6 4 3 42 9 0.0 6 7 2 43 7 0.0 6 6 0 1 44 10 0.0 6 9 1 45 8 0.0 6 7 1 46 11 0.0 6 10 1 47 8 0.0 6 8 48 16 0.0 6 12 4 49 19 0.0 6 17 2 50 19 0.0 6 16 2 0 0 0 1 51 10 0.0 6 9 1 52 12 0.0 6 12 53 14 0.0 6 11 3 54 12 0.0 6 9 3 55 19 0.0 6 17 2 56 27 0.0 6 21 4 1 1 57 17 0.0 6 16 1 58 15 0.0 6 12 2 1 59 19 0.0 6 18 0 0 0 1 60 15 0.0 6 14 1 61 9 0.0 6 8 0 1 62 10 0.0 6 10 63 9 0.0 6 9 64 11 0.0 6 11 65 3 0.0 6 2 1 66 9 0.0 6 9 67 11 0.0 6 11 68 19 0.0 6 16 1 1 0 1 69 22 0.0 6 21 1 70 97 0.0 6 95 2 71 26 0.0 6 23 3 72 53 0.0 6 49 4 73 12 0.0 6 12 74 27 0.0 6 27 75 20 0.0 6 18 2 76 12 0.0 6 11 1 77 18 0.0 6 18 78 19 0.0 6 17 2 79 11 0.0 6 10 1 80 5 0.0 6 5 81 18 0.0 6 16 2 82 21 0.0 6 18 2 0 0 1 83 38 0.0 6 37 1 84 26 0.0 6 24 1 0 0 1 85 41 0.0 6 40 1 86 36 0.0 6 36 87 40 0.0 6 38 2 88 18 0.0 6 18 89 22 0.0 6 19 2 0 1 90 40 0.0 6 37 3 91 23 0.0 6 22 1 92 27 0.0 6 26 1 93 32 0.0 6 28 4 94 66 0.0 6 61 4 1 95 29 0.0 6 27 1 1 96 45 0.0 6 42 2 1 97 35 0.0 6 32 2 1 98 27 0.0 6 27 99 37 0.0 6 33 3 1 100 40 0.0 6 39 1 101 29 0.0 6 26 1 0 1 1 102 65 0.0 6 60 5 103 71 0.0 6 67 3 0 1 104 69 0.0 6 64 4 0 0 0 0 1 105 51 0.0 6 49 2 106 37 0.0 6 34 2 0 0 1 107 81 0.0 6 74 7 108 41 0.0 6 37 4 109 39 0.0 6 36 3 110 42 0.0 6 37 5 111 35 0.0 6 32 2 0 0 1 112 38 0.0 6 35 1 1 0 1 113 32 0.0 6 28 3 0 1 114 39 0.0 6 38 1 115 53 0.0 6 48 5 116 52 0.0 6 50 2 117 58 0.0 6 57 1 118 56 0.0 6 55 1 119 60 0.0 6 57 3 120 92 0.0 6 85 6 0 0 1 121 79 0.0 6 74 5 122 103 0.0 6 99 2 2 123 96 0.0 6 92 2 2 124 116 0.0 6 107 9 125 81 0.0 6 80 1 126 68 0.0 6 63 5 127 78 0.0 6 76 2 128 75 0.0 6 72 1 2 129 110 0.0 6 107 2 0 0 1 130 106 0.0 6 99 7 131 104 0.0 6 97 7 132 191 0.0 6 183 8 133 158 0.0 6 151 6 0 0 1 134 189 0.0 6 181 8 135 259 0.0 6 248 8 3 136 176 0.0 6 171 4 0 0 1 137 251 0.0 6 244 6 0 0 0 1 138 233 0.0 6 228 5 139 228 0.0 6 222 6 140 254 0.0 6 252 2 141 218 0.0 6 215 3 142 324 0.0 6 311 11 2 143 295 0.0 6 284 11 144 426 0.0 6 418 8 145 341 0.0 6 334 5 1 0 1 146 298 0.0 6 290 6 1 1 147 283 0.0 6 276 6 1 148 320 0.0 6 317 3 149 366 0.0 6 354 10 1 0 1 150 451 0.0 6 443 6 0 0 1 1 151 342 0.0 6 336 6 152 551 0.0 6 536 13 1 0 1 153 574 0.0 6 559 13 1 0 0 1 154 462 0.0 6 453 8 0 1 155 541 0.0 6 526 14 1 156 482 0.0 6 466 16 157 537 0.0 6 523 11 0 0 1 1 1 158 570 0.0 6 551 18 1 159 282 0.0 6 272 10 160 594 0.0 6 580 14 161 442 0.0 6 430 11 1 162 379 0.0 6 373 6 163 423 0.0 6 413 9 1 164 359 0.0 6 352 6 0 0 1 165 418 0.0 6 408 10 166 544 0.0 6 528 14 2 167 271 0.0 6 266 5 168 605 0.0 6 596 8 0 1 169 262 0.0 6 256 6 170 291 0.0 6 279 10 2 171 576 0.0 6 565 11 172 273 0.0 6 263 9 1 173 262 0.0 6 256 5 0 0 1 174 402 0.0 6 388 13 0 0 0 1 175 703 0.0 6 683 17 1 0 2 176 458 0.0 6 449 9 177 605 0.0 6 590 14 0 0 1 178 400 0.0 6 382 15 3 179 599 0.0 6 584 15 180 490 0.0 6 476 14 181 823 0.0 6 803 19 0 0 1 182 329 0.0 6 315 14 183 613 0.0 6 600 13 184 207 0.0 6 204 3 185 277 0.0 6 263 14 186 838 0.0 6 808 28 1 0 1 187 992 0.0 6 953 36 1 0 2 188 1162 0.0 6 1125 35 2 189 1036 0.0 6 1005 31 190 1299 0.0 6 1272 27 191 1158 0.0 6 1127 28 1 1 1 192 1032 0.0 6 996 34 1 0 1 193 592 0.0 6 568 23 1 194 912 0.0 6 883 22 5 1 1 195 950 0.0 6 917 29 3 1 196 2736 0.0 6 2661 72 0 0 3 197 564 0.0 6 544 17 3 198 1193 0.0 6 1153 40 199 3143 0.0 6 3056 85 0 0 2 200 559 0.0 6 544 13 0 1 1 201 1073 0.0 6 1045 26 0 0 2 202 613 0.0 6 597 14 1 0 1 203 484 0.0 6 475 9 204 523 0.0 6 515 8 205 242 0.0 6 235 7 206 290 0.0 6 284 6 207 173 0.0 6 163 10 208 241 0.0 6 232 9 209 275 0.0 6 270 5 210 236 0.0 6 232 4 211 396 0.0 6 386 9 1 212 401 0.0 6 391 9 1 213 71 0.0 6 68 3 214 185 0.0 6 180 5 215 146 0.0 6 142 4 216 521 0.0 6 511 10 217 604 0.0 6 591 13 218 221 0.0 6 212 9 219 265 0.0 6 253 11 1 220 184 0.0 6 177 7 221 326 0.0 6 319 7 222 689 0.0 6 672 16 1 223 686 0.0 6 667 18 0 0 1 224 84 0.0 6 78 5 1 225 230 0.0 6 223 7 226 118 0.0 6 116 2 227 144 0.0 6 139 5 228 85 0.0 6 84 0 0 0 1 229 129 0.0 6 127 2 230 51 0.0 6 48 3 231 89 0.0 6 88 1 232 115 0.0 6 111 4 233 154 0.0 6 150 4 234 63 0.0 6 63 235 54 0.0 6 53 1 236 19 0.0 6 18 1 237 27 0.0 6 27 238 17 0.0 6 16 1 239 14 0.0 6 14 240 45 0.0 6 45 241 81 0.0 6 77 4 242 147 0.0 6 143 4 243 86 0.0 6 84 2 244 175 0.0 6 175 245 33 0.0 6 33 246 13 0.0 6 13 247 8 0.0 6 8 248 18 0.0 6 18 249 10 0.0 6 9 1 250 17 0.0 6 17 251 31 0.0 6 30 1 252 1 0.0 6 1 253 3 0.0 6 3 254 4 0.0 6 4 255 2 0.0 6 2 280 1 0.0 6 1 281 8 0.0 6 6 2 282 20 0.0 6 19 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 211 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 68 2 0.0 6 2 69 2 0.0 6 2 70 7 0.0 6 7 71 5 0.0 6 5 73 2 0.0 6 2 74 7 0.0 6 7 75 2 0.0 6 2 76 4 0.0 6 4 77 1 0.0 6 1 78 4 0.0 6 4 79 3 0.0 6 3 80 1 0.0 6 1 81 5 0.0 6 5 82 2 0.0 6 2 83 1 0.0 6 1 84 2 0.0 6 2 85 1 0.0 6 1 87 8 0.0 6 8 88 4 0.0 6 4 89 1 0.0 6 1 91 3 0.0 6 3 92 1 0.0 6 1 94 2 0.0 6 2 95 1 0.0 6 1 97 2 0.0 6 2 98 1 0.0 6 1 99 7 0.0 6 7 100 1 0.0 6 1 103 3 0.0 6 3 104 2 0.0 6 1 1 106 2 0.0 6 2 107 1 0.0 6 1 108 2 0.0 6 2 109 2 0.0 6 2 110 1 0.0 6 1 111 2 0.0 6 2 112 3 0.0 6 3 113 2 0.0 6 2 114 1 0.0 6 1 116 2 0.0 6 2 118 2 0.0 6 2 119 1 0.0 6 1 120 1 0.0 6 1 121 1 0.0 6 1 123 2 0.0 6 2 124 1 0.0 6 1 125 2 0.0 6 2 126 11 0.0 6 11 127 3 0.0 6 3 128 3 0.0 6 3 129 1 0.0 6 1 130 2 0.0 6 2 131 1 0.0 6 1 133 1 0.0 6 1 134 3 0.0 6 2 0 0 0 1 136 2 0.0 6 2 137 2 0.0 6 2 138 2 0.0 6 2 140 2 0.0 6 2 141 2 0.0 6 2 142 1 0.0 6 1 143 6 0.0 6 6 144 2 0.0 6 2 147 2 0.0 6 2 148 2 0.0 6 2 149 1 0.0 6 1 150 1 0.0 6 1 151 1 0.0 6 1 154 3 0.0 6 3 156 1 0.0 6 1 158 3 0.0 6 3 159 1 0.0 6 1 160 2 0.0 6 2 162 2 0.0 6 2 163 4 0.0 6 4 164 4 0.0 6 4 165 1 0.0 6 1 166 2 0.0 6 2 168 2 0.0 6 2 172 3 0.0 6 3 173 2 0.0 6 2 174 1 0.0 6 1 176 1 0.0 6 1 177 1 0.0 6 1 180 1 0.0 6 1 181 1 0.0 6 1 182 1 0.0 6 1 184 1 0.0 6 1 185 1 0.0 6 1 190 1 0.0 6 1 193 1 0.0 6 1 195 2 0.0 6 2 205 3 0.0 6 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_07_192_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_07_419_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_07_192_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_07_419_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_08_193_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_08_420_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_08_193_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_08_420_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_09_194_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_09_421_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_09_194_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_09_421_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_10_195_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_10_422_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_10_195_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_10_422_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_11_196_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_11_423_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_11_196_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_11_423_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_12_197_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_12_424_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_12_197_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_12_424_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_13_198_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_20-Rev_13_425_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_13_198_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_20-Rev_13_425_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_07_199_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_07_426_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_07_199_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_07_426_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_08_200_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_08_427_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_08_200_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_08_427_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_09_201_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_09_428_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_09_201_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_09_428_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_10_202_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_10_429_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_10_202_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_10_429_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_11_203_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_11_430_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_11_203_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_11_430_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_12_204_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_12_431_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_12_204_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_12_431_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.67 s (86 µs/read; 0.70 M reads/minute). === Summary === Total read pairs processed: 42,868 Read 1 with adapter: 42,866 (100.0%) Read 2 with adapter: 42,841 (99.9%) == Read fate breakdown == Pairs that were too short: 8 (0.0%) Pairs discarded as untrimmed: 29 (0.1%) Pairs written (passing filters): 42,831 (99.9%) Total basepairs processed: 19,801,965 bp Read 1: 10,928,005 bp Read 2: 8,873,960 bp Total written (filtered): 2,691,195 bp (13.6%) Read 1: 1,335,866 bp Read 2: 1,355,329 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 42031 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 167.5 1 0 1 6 1 10.5 1 1 9 1 0.2 2 0 1 12 1 0.0 3 1 14 1 0.0 4 1 16 1 0.0 4 0 1 20 18 0.0 6 18 21 15 0.0 6 15 22 10 0.0 6 10 23 11 0.0 6 11 24 12 0.0 6 12 25 10 0.0 6 9 1 26 5 0.0 6 5 27 6 0.0 6 5 1 28 2 0.0 6 2 29 4 0.0 6 3 1 30 1 0.0 6 1 31 1 0.0 6 1 33 4 0.0 6 4 34 2 0.0 6 2 35 3 0.0 6 2 1 36 1 0.0 6 1 38 4 0.0 6 4 39 2 0.0 6 2 40 1 0.0 6 1 42 1 0.0 6 1 44 2 0.0 6 2 46 1 0.0 6 1 47 1 0.0 6 1 48 4 0.0 6 4 51 1 0.0 6 1 53 3 0.0 6 3 54 2 0.0 6 2 55 1 0.0 6 1 57 2 0.0 6 1 1 58 1 0.0 6 1 61 2 0.0 6 2 62 1 0.0 6 1 63 2 0.0 6 2 64 1 0.0 6 1 65 1 0.0 6 1 66 3 0.0 6 3 67 1 0.0 6 1 68 5 0.0 6 4 1 69 2 0.0 6 2 72 3 0.0 6 3 73 4 0.0 6 4 74 1 0.0 6 0 1 75 3 0.0 6 3 76 3 0.0 6 3 77 4 0.0 6 4 78 1 0.0 6 1 79 1 0.0 6 1 80 6 0.0 6 6 81 4 0.0 6 3 1 82 1 0.0 6 1 83 2 0.0 6 2 84 4 0.0 6 4 85 9 0.0 6 9 86 7 0.0 6 7 87 2 0.0 6 2 88 2 0.0 6 2 89 3 0.0 6 3 91 2 0.0 6 2 92 5 0.0 6 5 93 1 0.0 6 1 94 7 0.0 6 7 96 2 0.0 6 2 98 2 0.0 6 2 99 7 0.0 6 7 100 5 0.0 6 5 101 2 0.0 6 2 102 3 0.0 6 3 103 11 0.0 6 11 104 7 0.0 6 6 0 0 0 0 1 105 4 0.0 6 4 106 6 0.0 6 6 107 13 0.0 6 13 108 11 0.0 6 10 1 109 19 0.0 6 17 2 110 9 0.0 6 8 1 111 9 0.0 6 9 112 17 0.0 6 16 1 113 12 0.0 6 12 114 11 0.0 6 11 115 11 0.0 6 11 116 7 0.0 6 7 117 6 0.0 6 6 118 6 0.0 6 6 119 6 0.0 6 6 120 4 0.0 6 3 1 121 10 0.0 6 9 1 122 11 0.0 6 11 123 8 0.0 6 7 1 124 11 0.0 6 11 125 12 0.0 6 11 1 126 9 0.0 6 9 127 19 0.0 6 18 1 128 15 0.0 6 14 1 129 11 0.0 6 11 130 12 0.0 6 12 131 12 0.0 6 12 132 17 0.0 6 16 1 133 13 0.0 6 13 134 33 0.0 6 33 135 17 0.0 6 17 136 10 0.0 6 10 137 25 0.0 6 25 138 32 0.0 6 32 139 16 0.0 6 15 1 140 39 0.0 6 39 141 36 0.0 6 36 142 289 0.0 6 287 2 143 372 0.0 6 372 144 205 0.0 6 205 145 351 0.0 6 350 1 146 535 0.0 6 533 2 147 236 0.0 6 236 148 432 0.0 6 431 1 149 170 0.0 6 169 0 1 150 462 0.0 6 461 1 151 168 0.0 6 168 152 238 0.0 6 238 153 109 0.0 6 109 154 144 0.0 6 144 155 674 0.0 6 672 2 156 142 0.0 6 142 157 69 0.0 6 69 158 62 0.0 6 62 159 129 0.0 6 129 160 131 0.0 6 131 161 177 0.0 6 176 1 162 93 0.0 6 93 163 43 0.0 6 42 1 164 199 0.0 6 199 165 136 0.0 6 136 166 251 0.0 6 251 167 509 0.0 6 507 2 168 68 0.0 6 67 0 0 0 1 169 75 0.0 6 74 0 1 170 99 0.0 6 99 171 587 0.0 6 587 172 102 0.0 6 102 173 214 0.0 6 213 1 174 309 0.0 6 309 175 218 0.0 6 218 176 49 0.0 6 49 177 60 0.0 6 60 178 22 0.0 6 22 179 33 0.0 6 33 180 64 0.0 6 63 1 181 182 0.0 6 182 182 57 0.0 6 57 183 35 0.0 6 35 184 61 0.0 6 60 0 1 185 82 0.0 6 82 186 94 0.0 6 94 187 228 0.0 6 226 2 188 205 0.0 6 205 189 149 0.0 6 147 2 190 121 0.0 6 121 191 66 0.0 6 65 1 192 47 0.0 6 46 1 193 64 0.0 6 62 2 194 87 0.0 6 85 2 195 93 0.0 6 91 2 196 127 0.0 6 126 1 197 118 0.0 6 116 1 1 198 169 0.0 6 168 1 199 488 0.0 6 486 2 200 230 0.0 6 230 201 138 0.0 6 137 1 202 125 0.0 6 125 203 178 0.0 6 175 3 204 163 0.0 6 160 3 205 286 0.0 6 284 1 0 0 1 206 230 0.0 6 229 1 207 613 0.0 6 610 3 208 774 0.0 6 771 3 209 348 0.0 6 346 1 0 1 210 650 0.0 6 645 5 211 147 0.0 6 145 2 212 98 0.0 6 97 1 213 160 0.0 6 157 2 0 0 0 1 214 258 0.0 6 256 2 215 175 0.0 6 172 3 216 250 0.0 6 246 3 0 0 0 1 217 304 0.0 6 300 3 0 0 0 1 218 189 0.0 6 185 4 219 261 0.0 6 261 220 398 0.0 6 396 2 221 441 0.0 6 439 2 222 417 0.0 6 415 2 223 718 0.0 6 714 4 224 149 0.0 6 146 3 225 183 0.0 6 174 9 226 200 0.0 6 197 3 227 217 0.0 6 216 1 228 239 0.0 6 238 1 229 170 0.0 6 163 6 0 1 230 237 0.0 6 231 6 231 195 0.0 6 193 2 232 225 0.0 6 223 2 233 240 0.0 6 239 1 234 391 0.0 6 387 4 235 252 0.0 6 249 3 236 164 0.0 6 164 237 162 0.0 6 161 0 1 238 214 0.0 6 212 1 1 239 212 0.0 6 205 6 1 240 315 0.0 6 309 6 241 170 0.0 6 166 4 242 181 0.0 6 177 4 243 291 0.0 6 286 4 0 0 1 244 303 0.0 6 300 3 245 232 0.0 6 227 5 246 245 0.0 6 241 4 247 318 0.0 6 316 2 248 228 0.0 6 224 4 249 233 0.0 6 232 1 250 374 0.0 6 372 2 251 385 0.0 6 378 7 252 448 0.0 6 440 7 0 1 253 346 0.0 6 340 6 254 310 0.0 6 306 3 1 255 566 0.0 6 557 9 256 603 0.0 6 595 8 257 324 0.0 6 315 8 1 258 227 0.0 6 221 6 259 427 0.0 6 412 15 260 256 0.0 6 252 4 261 482 0.0 6 472 10 262 217 0.0 6 210 7 263 317 0.0 6 310 6 1 264 189 0.0 6 185 4 265 202 0.0 6 199 3 266 320 0.0 6 315 5 267 367 0.0 6 362 5 268 153 0.0 6 144 9 269 302 0.0 6 295 7 270 197 0.0 6 192 5 271 276 0.0 6 269 7 272 215 0.0 6 210 5 273 130 0.0 6 126 4 274 129 0.0 6 127 2 275 186 0.0 6 178 8 276 449 0.0 6 436 13 277 553 0.0 6 534 19 278 773 0.0 6 751 22 279 1274 0.0 6 1256 18 280 2455 0.0 6 2380 73 1 1 281 3560 0.0 6 3465 93 1 0 0 0 1 282 9 0.0 6 8 0 1 283 4 0.0 6 4 286 1 0.0 6 1 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 835 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 11 0.0 6 9 2 68 1 0.0 6 1 69 2 0.0 6 2 70 8 0.0 6 8 71 15 0.0 6 15 72 9 0.0 6 9 73 18 0.0 6 18 74 28 0.0 6 28 75 4 0.0 6 4 76 9 0.0 6 9 77 10 0.0 6 10 78 16 0.0 6 16 79 4 0.0 6 4 80 55 0.0 6 54 0 0 0 0 0 1 82 9 0.0 6 9 83 23 0.0 6 23 84 4 0.0 6 4 85 3 0.0 6 3 86 4 0.0 6 4 87 3 0.0 6 3 88 11 0.0 6 11 89 5 0.0 6 5 91 3 0.0 6 3 92 9 0.0 6 9 93 3 0.0 6 3 94 12 0.0 6 12 95 13 0.0 6 13 96 2 0.0 6 2 97 2 0.0 6 2 98 10 0.0 6 10 99 13 0.0 6 13 100 4 0.0 6 4 101 7 0.0 6 7 102 10 0.0 6 10 103 3 0.0 6 3 105 1 0.0 6 1 108 3 0.0 6 3 109 3 0.0 6 3 113 1 0.0 6 1 114 1 0.0 6 1 115 7 0.0 6 7 116 3 0.0 6 3 117 2 0.0 6 2 118 5 0.0 6 5 119 1 0.0 6 1 122 2 0.0 6 2 123 1 0.0 6 1 124 1 0.0 6 1 126 4 0.0 6 4 127 13 0.0 6 13 128 2 0.0 6 2 129 1 0.0 6 1 131 7 0.0 6 7 132 1 0.0 6 1 133 5 0.0 6 5 134 11 0.0 6 11 135 30 0.0 6 30 136 30 0.0 6 30 137 15 0.0 6 15 138 32 0.0 6 32 139 1 0.0 6 1 141 1 0.0 6 1 142 1 0.0 6 1 146 1 0.0 6 1 147 1 0.0 6 1 148 5 0.0 6 5 149 12 0.0 6 12 150 1 0.0 6 1 151 2 0.0 6 2 154 2 0.0 6 2 156 2 0.0 6 2 157 5 0.0 6 5 160 1 0.0 6 1 161 9 0.0 6 9 162 1 0.0 6 1 164 1 0.0 6 1 174 7 0.0 6 7 175 6 0.0 6 6 176 1 0.0 6 1 182 16 0.0 6 16 183 48 0.0 6 48 184 96 0.0 6 95 1 185 3 0.0 6 3 188 1 0.0 6 1 189 7 0.0 6 7 192 7 0.0 6 7 194 9 0.0 6 9 195 24 0.0 6 24 196 3 0.0 6 3 199 3 0.0 6 3 200 4 0.0 6 4 201 1 0.0 6 1 206 3 0.0 6 3 207 1 0.0 6 1 208 1 0.0 6 1 214 2 0.0 6 2 220 1 0.0 6 1 221 1 0.0 6 1 232 4 0.0 6 4 234 3 0.0 6 3 235 2 0.0 6 2 243 2 0.0 6 2 250 1 0.0 6 1 251 1 0.0 6 1 252 2 0.0 6 2 262 1 0.0 6 1 269 1 0.0 6 1 270 1 0.0 6 1 298 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 42060 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 669.8 0 0 2 6 1 10.5 1 1 10 1 0.0 3 1 11 2 0.0 3 2 12 5 0.0 3 5 13 7 0.0 3 7 14 3 0.0 4 3 15 1 0.0 4 1 16 3 0.0 4 3 17 7 0.0 5 7 18 10 0.0 5 8 2 19 7 0.0 5 7 20 15 0.0 6 15 21 17 0.0 6 15 2 22 21 0.0 6 20 1 23 14 0.0 6 14 24 7 0.0 6 7 25 11 0.0 6 11 26 8 0.0 6 8 27 3 0.0 6 3 28 6 0.0 6 4 2 29 5 0.0 6 4 1 30 7 0.0 6 6 1 31 4 0.0 6 4 32 7 0.0 6 7 33 1 0.0 6 1 34 5 0.0 6 4 1 35 6 0.0 6 6 36 1 0.0 6 1 37 12 0.0 6 10 2 38 8 0.0 6 8 39 2 0.0 6 1 1 40 6 0.0 6 6 41 9 0.0 6 8 1 42 6 0.0 6 6 43 2 0.0 6 2 44 5 0.0 6 5 45 7 0.0 6 6 0 0 0 1 46 17 0.0 6 15 2 47 12 0.0 6 9 3 48 8 0.0 6 7 1 49 8 0.0 6 6 2 50 8 0.0 6 7 1 51 10 0.0 6 9 1 52 13 0.0 6 13 53 3 0.0 6 3 54 9 0.0 6 9 55 10 0.0 6 8 2 56 7 0.0 6 5 2 57 14 0.0 6 13 0 1 58 8 0.0 6 7 1 59 6 0.0 6 4 2 60 10 0.0 6 8 2 61 10 0.0 6 9 1 62 5 0.0 6 5 63 8 0.0 6 8 64 7 0.0 6 7 65 8 0.0 6 7 1 66 6 0.0 6 5 1 67 29 0.0 6 28 1 68 83 0.0 6 77 5 1 69 21 0.0 6 19 2 70 36 0.0 6 34 2 71 14 0.0 6 13 1 72 30 0.0 6 29 1 73 4 0.0 6 4 74 10 0.0 6 8 2 75 13 0.0 6 11 1 0 1 76 13 0.0 6 12 1 77 8 0.0 6 8 78 16 0.0 6 14 2 79 14 0.0 6 14 80 15 0.0 6 14 1 81 14 0.0 6 14 82 22 0.0 6 19 3 83 30 0.0 6 27 3 84 31 0.0 6 29 2 85 22 0.0 6 21 1 86 9 0.0 6 8 1 87 16 0.0 6 13 3 88 15 0.0 6 14 1 89 18 0.0 6 17 1 90 20 0.0 6 19 1 91 21 0.0 6 19 1 1 92 22 0.0 6 22 93 26 0.0 6 26 94 38 0.0 6 37 1 95 25 0.0 6 23 2 96 21 0.0 6 18 3 97 15 0.0 6 15 98 23 0.0 6 21 1 1 99 23 0.0 6 22 1 100 21 0.0 6 20 1 101 40 0.0 6 37 2 0 1 102 32 0.0 6 30 1 0 0 1 103 37 0.0 6 36 0 1 104 32 0.0 6 28 4 105 57 0.0 6 57 106 38 0.0 6 37 1 107 37 0.0 6 37 108 29 0.0 6 28 1 109 40 0.0 6 36 4 110 34 0.0 6 32 2 111 28 0.0 6 28 112 57 0.0 6 57 113 35 0.0 6 34 1 114 42 0.0 6 41 1 115 41 0.0 6 38 3 116 47 0.0 6 46 1 117 39 0.0 6 39 118 53 0.0 6 52 1 119 55 0.0 6 53 2 120 46 0.0 6 45 1 121 67 0.0 6 67 122 53 0.0 6 52 1 123 48 0.0 6 47 0 1 124 65 0.0 6 64 0 1 125 66 0.0 6 62 3 1 126 66 0.0 6 65 1 127 89 0.0 6 88 1 128 74 0.0 6 73 1 129 94 0.0 6 93 1 130 63 0.0 6 62 1 131 85 0.0 6 83 2 132 109 0.0 6 108 1 133 111 0.0 6 109 2 134 182 0.0 6 175 6 1 135 189 0.0 6 185 4 136 188 0.0 6 185 3 137 199 0.0 6 196 2 1 138 361 0.0 6 356 5 139 388 0.0 6 384 4 140 329 0.0 6 322 7 141 448 0.0 6 445 3 142 591 0.0 6 586 4 1 143 403 0.0 6 395 8 144 485 0.0 6 483 2 145 335 0.0 6 330 5 146 475 0.0 6 471 4 147 291 0.0 6 285 5 1 148 367 0.0 6 360 7 149 312 0.0 6 303 8 0 0 0 0 1 150 446 0.0 6 442 4 151 849 0.0 6 843 6 152 377 0.0 6 370 7 153 340 0.0 6 333 7 154 373 0.0 6 373 155 311 0.0 6 305 6 156 378 0.0 6 372 5 0 1 157 363 0.0 6 358 5 158 330 0.0 6 323 6 1 159 290 0.0 6 285 3 1 0 0 0 1 160 403 0.0 6 400 3 161 328 0.0 6 325 3 162 399 0.0 6 394 5 163 450 0.0 6 443 7 164 309 0.0 6 305 3 1 165 377 0.0 6 366 9 2 166 388 0.0 6 385 3 167 497 0.0 6 491 4 2 168 365 0.0 6 362 3 169 379 0.0 6 370 8 0 0 1 170 389 0.0 6 382 7 171 382 0.0 6 376 6 172 306 0.0 6 301 4 0 1 173 423 0.0 6 416 6 1 174 385 0.0 6 377 8 175 399 0.0 6 386 9 0 2 2 176 384 0.0 6 376 7 0 1 177 635 0.0 6 621 14 178 529 0.0 6 524 5 179 450 0.0 6 441 8 0 0 1 180 404 0.0 6 398 6 181 469 0.0 6 459 10 182 339 0.0 6 334 3 0 0 2 183 600 0.0 6 589 9 2 184 692 0.0 6 686 6 185 638 0.0 6 624 13 0 0 0 0 1 186 680 0.0 6 666 14 187 454 0.0 6 447 6 0 1 188 531 0.0 6 524 7 189 614 0.0 6 597 15 1 0 0 0 1 190 562 0.0 6 548 14 191 605 0.0 6 590 14 1 192 551 0.0 6 542 9 193 461 0.0 6 454 7 194 519 0.0 6 510 7 1 1 195 525 0.0 6 509 16 196 821 0.0 6 798 19 2 0 2 197 474 0.0 6 466 8 198 636 0.0 6 617 18 0 0 1 199 561 0.0 6 549 12 200 518 0.0 6 500 17 0 0 1 201 731 0.0 6 717 14 202 396 0.0 6 386 10 203 377 0.0 6 369 7 1 204 353 0.0 6 345 8 205 308 0.0 6 298 10 206 280 0.0 6 270 9 1 207 282 0.0 6 276 6 208 430 0.0 6 421 9 209 218 0.0 6 215 3 210 320 0.0 6 309 10 1 211 335 0.0 6 329 5 0 0 1 212 470 0.0 6 464 5 0 0 1 213 169 0.0 6 166 3 214 244 0.0 6 240 4 215 272 0.0 6 262 10 216 245 0.0 6 239 6 217 179 0.0 6 175 4 218 206 0.0 6 202 3 0 0 1 219 120 0.0 6 115 4 0 0 1 220 154 0.0 6 150 4 221 196 0.0 6 191 5 222 247 0.0 6 243 4 223 181 0.0 6 176 4 1 224 85 0.0 6 85 225 133 0.0 6 133 226 185 0.0 6 180 5 227 290 0.0 6 285 5 228 187 0.0 6 182 5 229 161 0.0 6 153 7 1 230 506 0.0 6 487 18 0 0 1 231 116 0.0 6 109 5 1 0 1 232 242 0.0 6 237 5 233 75 0.0 6 73 2 234 92 0.0 6 89 3 235 62 0.0 6 62 236 40 0.0 6 40 237 46 0.0 6 45 1 238 51 0.0 6 49 2 239 29 0.0 6 29 240 33 0.0 6 31 2 241 36 0.0 6 36 242 53 0.0 6 51 2 243 37 0.0 6 34 2 1 244 22 0.0 6 20 2 245 37 0.0 6 36 1 246 36 0.0 6 35 1 247 24 0.0 6 24 248 19 0.0 6 17 2 249 54 0.0 6 52 2 250 11 0.0 6 11 251 16 0.0 6 14 2 252 17 0.0 6 17 253 12 0.0 6 12 254 37 0.0 6 37 255 9 0.0 6 9 256 10 0.0 6 10 258 1 0.0 6 1 261 1 0.0 6 1 267 1 0.0 6 1 277 1 0.0 6 1 278 4 0.0 6 3 1 279 31 0.0 6 29 2 280 135 0.0 6 132 3 281 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 781 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 57 1 0.0 6 1 69 2 0.0 6 2 70 1 0.0 6 1 71 9 0.0 6 9 72 9 0.0 6 9 73 9 0.0 6 9 74 16 0.0 6 16 75 14 0.0 6 14 76 12 0.0 6 12 77 15 0.0 6 15 78 8 0.0 6 7 0 0 0 1 79 29 0.0 6 29 80 7 0.0 6 7 81 9 0.0 6 9 82 5 0.0 6 5 83 11 0.0 6 11 84 37 0.0 6 37 86 1 0.0 6 1 87 3 0.0 6 3 88 4 0.0 6 4 89 5 0.0 6 5 90 8 0.0 6 8 91 4 0.0 6 4 93 7 0.0 6 7 94 7 0.0 6 7 95 10 0.0 6 10 96 13 0.0 6 13 99 10 0.0 6 10 100 23 0.0 6 23 101 5 0.0 6 5 102 11 0.0 6 11 103 20 0.0 6 20 104 7 0.0 6 7 105 1 0.0 6 1 109 1 0.0 6 1 110 4 0.0 6 4 114 2 0.0 6 2 115 2 0.0 6 2 116 13 0.0 6 13 117 4 0.0 6 4 118 3 0.0 6 3 119 6 0.0 6 6 123 2 0.0 6 2 125 2 0.0 6 2 127 6 0.0 6 6 128 11 0.0 6 11 129 3 0.0 6 3 130 1 0.0 6 0 1 131 1 0.0 6 1 133 1 0.0 6 1 134 3 0.0 6 3 135 6 0.0 6 6 136 23 0.0 6 23 137 25 0.0 6 25 138 8 0.0 6 8 139 39 0.0 6 39 140 3 0.0 6 3 142 1 0.0 6 0 0 0 0 1 146 1 0.0 6 1 149 5 0.0 6 5 150 19 0.0 6 19 152 1 0.0 6 1 154 1 0.0 6 1 155 3 0.0 6 3 158 2 0.0 6 2 159 1 0.0 6 1 161 1 0.0 6 1 162 2 0.0 6 2 163 1 0.0 6 1 164 1 0.0 6 1 172 1 0.0 6 1 173 1 0.0 6 0 1 175 2 0.0 6 2 176 8 0.0 6 8 182 2 0.0 6 1 1 183 11 0.0 6 11 184 46 0.0 6 46 185 86 0.0 6 86 186 3 0.0 6 2 0 1 189 4 0.0 6 1 0 0 0 2 1 190 7 0.0 6 7 193 7 0.0 6 7 195 11 0.0 6 11 196 37 0.0 6 37 198 1 0.0 6 1 200 2 0.0 6 2 201 3 0.0 6 3 207 1 0.0 6 1 208 2 0.0 6 2 209 3 0.0 6 3 216 1 0.0 6 1 222 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_13_205_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_13_432_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_13_205_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_13_432_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.03 s (69 µs/read; 0.87 M reads/minute). === Summary === Total read pairs processed: 73,107 Read 1 with adapter: 73,099 (100.0%) Read 2 with adapter: 73,078 (100.0%) == Read fate breakdown == Pairs that were too short: 50 (0.1%) Pairs discarded as untrimmed: 35 (0.0%) Pairs written (passing filters): 73,022 (99.9%) Total basepairs processed: 31,915,232 bp Read 1: 17,654,924 bp Read 2: 14,260,308 bp Total written (filtered): 4,572,608 bp (14.3%) Read 1: 2,322,313 bp Read 2: 2,250,295 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 70609 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 3 1142.3 0 0 3 4 2 285.6 1 1 1 5 1 71.4 1 1 6 2 17.8 1 0 1 1 7 2 4.5 2 1 0 1 9 5 0.3 2 5 10 3 0.1 3 2 1 11 2 0.0 3 2 14 1 0.0 4 1 15 1 0.0 4 1 16 1 0.0 4 1 18 3 0.0 5 3 19 1 0.0 5 0 1 20 61 0.0 6 56 5 21 31 0.0 6 29 2 22 35 0.0 6 32 3 23 22 0.0 6 21 1 24 28 0.0 6 27 1 25 27 0.0 6 26 1 26 13 0.0 6 12 1 27 9 0.0 6 9 28 10 0.0 6 9 1 29 12 0.0 6 11 1 30 8 0.0 6 8 31 5 0.0 6 5 32 6 0.0 6 6 33 4 0.0 6 4 34 6 0.0 6 5 1 36 3 0.0 6 3 37 3 0.0 6 3 38 4 0.0 6 4 39 10 0.0 6 10 40 2 0.0 6 2 41 7 0.0 6 7 42 1 0.0 6 1 43 1 0.0 6 1 44 10 0.0 6 10 45 2 0.0 6 2 46 4 0.0 6 3 1 47 1 0.0 6 1 48 4 0.0 6 4 49 15 0.0 6 14 1 50 14 0.0 6 13 1 51 17 0.0 6 17 52 6 0.0 6 6 53 5 0.0 6 4 1 54 5 0.0 6 4 1 55 9 0.0 6 7 2 56 2 0.0 6 2 57 3 0.0 6 3 58 5 0.0 6 5 59 1 0.0 6 1 60 3 0.0 6 3 61 5 0.0 6 5 62 7 0.0 6 7 63 15 0.0 6 14 1 64 21 0.0 6 21 65 26 0.0 6 24 2 66 7 0.0 6 6 1 67 12 0.0 6 10 2 68 9 0.0 6 9 69 8 0.0 6 8 70 6 0.0 6 5 1 71 9 0.0 6 8 1 72 10 0.0 6 10 73 14 0.0 6 13 1 74 10 0.0 6 9 0 0 0 0 1 75 11 0.0 6 10 0 0 1 76 11 0.0 6 11 77 12 0.0 6 10 1 0 1 78 7 0.0 6 7 79 7 0.0 6 7 80 10 0.0 6 10 81 9 0.0 6 8 1 82 9 0.0 6 9 83 8 0.0 6 7 0 0 0 1 84 12 0.0 6 12 85 11 0.0 6 11 86 6 0.0 6 6 87 7 0.0 6 7 88 6 0.0 6 6 89 5 0.0 6 5 90 4 0.0 6 4 91 6 0.0 6 6 92 4 0.0 6 4 93 5 0.0 6 5 94 6 0.0 6 6 95 3 0.0 6 3 96 9 0.0 6 9 97 5 0.0 6 5 98 6 0.0 6 6 99 6 0.0 6 5 1 100 7 0.0 6 6 1 101 8 0.0 6 8 102 11 0.0 6 10 0 1 103 9 0.0 6 9 104 10 0.0 6 10 105 15 0.0 6 15 106 12 0.0 6 11 1 107 16 0.0 6 16 108 13 0.0 6 13 109 15 0.0 6 14 1 110 11 0.0 6 10 1 111 8 0.0 6 7 1 112 14 0.0 6 14 113 17 0.0 6 17 114 23 0.0 6 23 115 16 0.0 6 16 116 17 0.0 6 17 117 18 0.0 6 18 118 19 0.0 6 19 119 14 0.0 6 14 120 21 0.0 6 21 121 26 0.0 6 25 0 0 0 1 122 12 0.0 6 11 1 123 15 0.0 6 14 1 124 20 0.0 6 20 125 18 0.0 6 18 126 9 0.0 6 9 127 23 0.0 6 22 1 128 18 0.0 6 18 129 21 0.0 6 18 3 130 25 0.0 6 25 131 15 0.0 6 15 132 21 0.0 6 20 0 1 133 23 0.0 6 23 134 22 0.0 6 22 135 24 0.0 6 23 0 0 0 1 136 23 0.0 6 23 137 26 0.0 6 26 138 26 0.0 6 26 139 21 0.0 6 21 140 56 0.0 6 56 141 312 0.0 6 311 1 142 102 0.0 6 99 3 143 200 0.0 6 200 144 75 0.0 6 75 145 58 0.0 6 58 146 134 0.0 6 132 1 1 147 614 0.0 6 610 3 0 0 1 148 239 0.0 6 239 149 71 0.0 6 71 150 106 0.0 6 106 151 136 0.0 6 135 1 152 344 0.0 6 343 1 153 614 0.0 6 612 1 1 154 666 0.0 6 665 1 155 957 0.0 6 957 156 211 0.0 6 207 4 157 187 0.0 6 185 1 0 0 1 158 150 0.0 6 149 1 159 675 0.0 6 674 1 160 726 0.0 6 725 1 161 139 0.0 6 139 162 416 0.0 6 413 2 0 0 1 163 570 0.0 6 568 0 0 0 0 2 164 148 0.0 6 147 1 165 128 0.0 6 126 1 0 0 0 1 166 686 0.0 6 680 3 0 1 1 1 167 217 0.0 6 216 1 168 95 0.0 6 94 1 169 298 0.0 6 296 1 1 170 870 0.0 6 868 1 1 171 167 0.0 6 166 0 1 172 89 0.0 6 88 0 0 0 0 1 173 87 0.0 6 85 1 0 0 0 0 1 174 134 0.0 6 132 1 0 0 0 0 1 175 97 0.0 6 96 1 176 99 0.0 6 98 1 177 141 0.0 6 141 178 291 0.0 6 291 179 111 0.0 6 111 180 179 0.0 6 178 1 181 834 0.0 6 834 182 284 0.0 6 283 1 183 93 0.0 6 93 184 183 0.0 6 183 185 102 0.0 6 102 186 129 0.0 6 128 1 187 169 0.0 6 168 1 188 266 0.0 6 266 189 1227 0.0 6 1225 2 190 230 0.0 6 228 1 0 0 1 191 162 0.0 6 161 1 192 346 0.0 6 345 1 193 1642 0.0 6 1640 1 0 0 0 1 194 443 0.0 6 442 1 195 338 0.0 6 336 1 0 1 196 714 0.0 6 711 3 197 270 0.0 6 269 1 198 325 0.0 6 323 1 0 0 0 0 1 199 481 0.0 6 478 3 200 396 0.0 6 389 7 201 390 0.0 6 389 0 0 0 0 0 1 202 353 0.0 6 351 2 203 490 0.0 6 488 1 0 0 0 1 204 400 0.0 6 396 4 205 1077 0.0 6 1075 2 206 482 0.0 6 478 3 0 0 0 1 207 566 0.0 6 564 2 208 1045 0.0 6 1041 2 0 0 1 0 1 209 972 0.0 6 967 4 1 210 1453 0.0 6 1447 6 211 2626 0.0 6 2619 4 1 0 2 212 607 0.0 6 604 3 213 869 0.0 6 865 2 1 0 1 214 748 0.0 6 746 1 0 1 215 1649 0.0 6 1644 4 0 0 0 1 216 562 0.0 6 559 2 0 0 0 0 1 217 864 0.0 6 859 5 218 878 0.0 6 875 3 219 633 0.0 6 630 2 0 1 220 624 0.0 6 622 2 221 661 0.0 6 657 4 222 544 0.0 6 539 5 223 513 0.0 6 512 1 224 832 0.0 6 827 5 225 644 0.0 6 640 4 226 908 0.0 6 904 3 0 0 1 227 1307 0.0 6 1301 6 228 1556 0.0 6 1555 1 229 2208 0.0 6 2204 3 1 230 797 0.0 6 794 3 231 480 0.0 6 478 2 232 792 0.0 6 790 2 233 713 0.0 6 710 2 0 0 1 234 1103 0.0 6 1097 6 235 1857 0.0 6 1853 4 236 3262 0.0 6 3259 3 237 236 0.0 6 234 2 238 142 0.0 6 142 239 154 0.0 6 153 1 240 185 0.0 6 181 4 241 208 0.0 6 207 1 242 233 0.0 6 231 2 243 272 0.0 6 266 5 1 244 383 0.0 6 380 3 245 422 0.0 6 421 1 246 705 0.0 6 703 2 247 313 0.0 6 312 1 248 467 0.0 6 466 1 249 357 0.0 6 356 1 250 326 0.0 6 325 1 251 526 0.0 6 523 3 252 423 0.0 6 417 6 253 364 0.0 6 362 1 0 0 1 254 193 0.0 6 193 255 241 0.0 6 239 2 256 305 0.0 6 303 2 257 454 0.0 6 451 3 258 335 0.0 6 335 259 618 0.0 6 617 0 0 0 1 260 234 0.0 6 233 1 261 218 0.0 6 217 1 262 215 0.0 6 213 2 263 225 0.0 6 222 2 1 264 300 0.0 6 296 4 265 288 0.0 6 286 1 0 0 0 1 266 358 0.0 6 357 1 267 238 0.0 6 238 268 258 0.0 6 257 1 269 545 0.0 6 542 3 270 156 0.0 6 152 4 271 181 0.0 6 176 5 272 82 0.0 6 78 3 0 0 1 273 96 0.0 6 92 3 1 274 90 0.0 6 90 275 113 0.0 6 111 1 0 0 0 0 1 276 157 0.0 6 154 3 277 189 0.0 6 187 2 278 215 0.0 6 213 2 279 236 0.0 6 233 3 280 687 0.0 6 678 7 0 0 0 1 1 281 501 0.0 6 499 1 0 0 1 282 2 0.0 6 2 283 1 0.0 6 1 288 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 2490 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 69 2 0.0 6 2 70 10 0.0 6 10 71 1 0.0 6 1 72 6 0.0 6 6 73 1 0.0 6 1 75 9 0.0 6 9 76 24 0.0 6 24 77 4 0.0 6 4 78 1 0.0 6 1 79 2 0.0 6 2 80 4 0.0 6 4 81 16 0.0 6 15 1 82 26 0.0 6 26 83 27 0.0 6 27 84 45 0.0 6 45 85 7 0.0 6 7 86 3 0.0 6 3 87 7 0.0 6 7 88 30 0.0 6 30 89 17 0.0 6 17 90 5 0.0 6 5 91 25 0.0 6 25 92 16 0.0 6 16 94 11 0.0 6 11 95 22 0.0 6 22 96 7 0.0 6 6 1 97 1 0.0 6 1 98 22 0.0 6 22 99 38 0.0 6 38 100 1 0.0 6 1 101 1 0.0 6 1 102 1 0.0 6 1 103 2 0.0 6 2 105 2 0.0 6 2 106 1 0.0 6 1 107 7 0.0 6 7 109 5 0.0 6 5 110 31 0.0 6 31 111 2 0.0 6 2 112 2 0.0 6 2 113 4 0.0 6 4 116 3 0.0 6 3 117 12 0.0 6 12 118 49 0.0 6 49 120 2 0.0 6 2 121 18 0.0 6 18 122 68 0.0 6 68 123 4 0.0 6 4 124 2 0.0 6 2 125 1 0.0 6 1 133 8 0.0 6 8 134 35 0.0 6 35 135 2 0.0 6 2 136 3 0.0 6 3 137 7 0.0 6 7 138 7 0.0 6 7 139 53 0.0 6 53 140 159 0.0 6 159 141 9 0.0 6 9 142 4 0.0 6 4 143 30 0.0 6 30 144 110 0.0 6 110 145 7 0.0 6 7 146 11 0.0 6 11 147 12 0.0 6 12 150 2 0.0 6 2 151 5 0.0 6 5 154 1 0.0 6 1 156 3 0.0 6 3 157 54 0.0 6 54 158 194 0.0 6 194 159 7 0.0 6 7 160 20 0.0 6 20 161 37 0.0 6 37 162 8 0.0 6 8 163 1 0.0 6 1 164 11 0.0 6 11 165 40 0.0 6 40 166 3 0.0 6 3 170 1 0.0 6 1 173 1 0.0 6 1 174 23 0.0 6 23 175 58 0.0 6 58 176 2 0.0 6 2 179 12 0.0 6 12 180 35 0.0 6 35 181 29 0.0 6 29 182 3 0.0 6 3 183 4 0.0 6 4 186 6 0.0 6 6 187 11 0.0 6 11 188 3 0.0 6 3 189 14 0.0 6 14 192 1 0.0 6 1 195 1 0.0 6 1 196 7 0.0 6 7 197 155 0.0 6 155 198 419 0.0 6 419 199 23 0.0 6 23 203 1 0.0 6 1 205 3 0.0 6 3 206 6 0.0 6 6 207 8 0.0 6 8 208 7 0.0 6 7 218 1 0.0 6 1 219 9 0.0 6 9 220 37 0.0 6 37 221 92 0.0 6 92 222 5 0.0 6 5 223 5 0.0 6 5 224 1 0.0 6 1 240 8 0.0 6 8 241 14 0.0 6 14 249 1 0.0 6 1 250 2 0.0 6 2 259 1 0.0 6 1 261 6 0.0 6 6 262 7 0.0 6 7 278 4 0.0 6 4 279 1 0.0 6 1 280 3 0.0 6 3 282 2 0.0 6 2 283 4 0.0 6 4 299 1 0.0 6 1 305 3 0.0 6 3 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 70846 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 285.6 1 0 1 11 3 0.0 3 2 1 12 1 0.0 3 1 13 12 0.0 3 11 1 14 7 0.0 4 7 15 2 0.0 4 2 16 12 0.0 4 11 1 17 14 0.0 5 14 18 10 0.0 5 10 19 8 0.0 5 8 20 55 0.0 6 53 2 21 29 0.0 6 26 3 22 22 0.0 6 22 23 24 0.0 6 22 2 24 13 0.0 6 12 1 25 17 0.0 6 17 26 13 0.0 6 12 1 27 14 0.0 6 14 28 13 0.0 6 13 29 8 0.0 6 7 1 30 15 0.0 6 14 1 31 14 0.0 6 12 1 1 32 9 0.0 6 9 33 7 0.0 6 7 34 5 0.0 6 3 2 35 13 0.0 6 11 2 36 3 0.0 6 3 37 10 0.0 6 7 3 38 9 0.0 6 8 1 39 14 0.0 6 12 2 40 9 0.0 6 7 1 1 41 9 0.0 6 8 1 42 13 0.0 6 12 1 43 11 0.0 6 9 2 44 12 0.0 6 12 45 11 0.0 6 11 46 11 0.0 6 10 1 47 16 0.0 6 16 48 12 0.0 6 12 49 15 0.0 6 13 2 50 16 0.0 6 15 1 51 19 0.0 6 19 52 14 0.0 6 13 1 53 13 0.0 6 12 1 54 14 0.0 6 12 2 55 10 0.0 6 9 1 56 20 0.0 6 19 1 57 22 0.0 6 17 4 0 1 58 25 0.0 6 21 4 59 15 0.0 6 14 1 60 19 0.0 6 18 1 61 19 0.0 6 17 2 62 17 0.0 6 17 63 10 0.0 6 8 2 64 27 0.0 6 22 4 0 1 65 32 0.0 6 31 1 66 30 0.0 6 27 3 67 37 0.0 6 34 2 1 68 37 0.0 6 35 2 69 132 0.0 6 127 3 1 1 70 45 0.0 6 42 2 1 71 52 0.0 6 50 2 72 33 0.0 6 31 0 1 0 0 1 73 41 0.0 6 38 3 74 42 0.0 6 38 3 0 1 75 31 0.0 6 31 76 24 0.0 6 24 77 32 0.0 6 31 1 78 21 0.0 6 15 5 0 1 79 46 0.0 6 45 1 80 37 0.0 6 34 1 2 81 38 0.0 6 36 2 82 35 0.0 6 31 3 0 1 83 54 0.0 6 52 2 84 48 0.0 6 44 3 0 1 85 60 0.0 6 56 4 86 36 0.0 6 35 1 87 32 0.0 6 30 1 1 88 32 0.0 6 32 89 28 0.0 6 27 1 90 42 0.0 6 41 1 91 43 0.0 6 41 2 92 70 0.0 6 67 2 0 1 93 43 0.0 6 39 3 1 94 46 0.0 6 45 1 95 59 0.0 6 54 5 96 41 0.0 6 41 97 53 0.0 6 53 98 57 0.0 6 52 5 99 52 0.0 6 49 3 100 53 0.0 6 52 1 101 44 0.0 6 41 3 102 51 0.0 6 50 1 103 84 0.0 6 81 2 0 1 104 66 0.0 6 63 1 0 0 1 0 1 105 80 0.0 6 77 3 106 146 0.0 6 138 8 107 71 0.0 6 65 6 108 70 0.0 6 69 1 109 110 0.0 6 105 5 110 100 0.0 6 98 1 0 1 111 77 0.0 6 73 4 112 96 0.0 6 95 1 113 84 0.0 6 79 4 0 1 114 107 0.0 6 102 4 0 1 115 90 0.0 6 87 3 116 97 0.0 6 95 2 117 124 0.0 6 121 3 118 119 0.0 6 116 2 0 0 0 0 1 119 141 0.0 6 137 4 120 170 0.0 6 165 2 1 1 0 1 121 148 0.0 6 146 2 122 185 0.0 6 179 6 123 126 0.0 6 122 4 124 169 0.0 6 166 2 0 0 0 0 1 125 129 0.0 6 126 3 126 139 0.0 6 133 6 127 206 0.0 6 201 4 0 0 0 1 128 217 0.0 6 210 7 129 187 0.0 6 182 4 0 1 130 216 0.0 6 208 8 131 369 0.0 6 357 10 2 132 231 0.0 6 226 5 133 317 0.0 6 312 4 0 1 134 409 0.0 6 400 8 1 135 363 0.0 6 353 10 136 350 0.0 6 344 4 0 2 137 356 0.0 6 351 4 0 0 0 0 1 138 529 0.0 6 526 3 139 349 0.0 6 343 5 1 140 486 0.0 6 482 3 0 1 141 346 0.0 6 337 8 1 142 400 0.0 6 394 5 0 1 143 411 0.0 6 405 4 1 0 0 0 1 144 872 0.0 6 864 7 0 1 145 625 0.0 6 616 6 0 3 146 541 0.0 6 531 9 0 1 147 505 0.0 6 493 11 0 0 0 1 148 643 0.0 6 627 12 0 2 1 1 149 772 0.0 6 756 13 1 1 1 150 1443 0.0 6 1413 25 3 2 151 1255 0.0 6 1240 12 1 2 152 1902 0.0 6 1880 19 1 2 153 1099 0.0 6 1069 22 1 6 1 154 1449 0.0 6 1413 33 1 1 0 1 155 1259 0.0 6 1229 22 0 6 0 1 1 156 1303 0.0 6 1279 22 1 1 157 1703 0.0 6 1684 16 1 2 158 967 0.0 6 952 14 0 0 1 159 1084 0.0 6 1067 15 0 1 1 160 1187 0.0 6 1170 14 0 3 161 1100 0.0 6 1082 15 1 1 1 162 1122 0.0 6 1080 36 2 4 163 1921 0.0 6 1878 36 1 5 0 0 1 164 867 0.0 6 851 15 1 165 774 0.0 6 751 22 0 1 166 1173 0.0 6 1143 29 0 1 167 825 0.0 6 822 3 168 1431 0.0 6 1425 5 1 169 743 0.0 6 738 4 1 170 1043 0.0 6 1036 7 171 962 0.0 6 962 172 922 0.0 6 911 11 173 766 0.0 6 759 7 174 1048 0.0 6 1043 4 0 1 175 1334 0.0 6 1330 4 176 840 0.0 6 837 3 177 702 0.0 6 702 178 1017 0.0 6 1011 6 179 493 0.0 6 489 2 0 0 0 1 1 180 626 0.0 6 621 5 181 688 0.0 6 684 3 0 1 182 800 0.0 6 796 3 1 183 540 0.0 6 536 3 0 0 0 1 184 726 0.0 6 721 5 185 1176 0.0 6 1170 5 0 1 186 1682 0.0 6 1664 16 1 1 187 1217 0.0 6 1203 14 188 1068 0.0 6 1067 1 189 923 0.0 6 917 6 190 950 0.0 6 944 4 2 191 909 0.0 6 906 2 0 1 192 934 0.0 6 929 4 0 1 193 503 0.0 6 501 2 194 560 0.0 6 556 4 195 480 0.0 6 476 4 196 403 0.0 6 401 2 197 364 0.0 6 359 4 0 1 198 877 0.0 6 873 4 199 554 0.0 6 551 2 0 1 200 477 0.0 6 474 3 201 376 0.0 6 374 2 202 328 0.0 6 327 1 203 334 0.0 6 333 1 204 299 0.0 6 296 3 205 272 0.0 6 268 3 0 1 206 310 0.0 6 307 3 207 329 0.0 6 328 1 208 380 0.0 6 379 1 209 278 0.0 6 277 1 210 208 0.0 6 206 2 211 272 0.0 6 271 1 212 172 0.0 6 171 1 213 221 0.0 6 219 2 214 127 0.0 6 125 2 215 270 0.0 6 266 4 216 119 0.0 6 118 1 217 102 0.0 6 101 1 218 84 0.0 6 79 4 0 1 219 136 0.0 6 135 1 220 85 0.0 6 84 1 221 97 0.0 6 96 1 222 58 0.0 6 57 1 223 87 0.0 6 85 2 224 114 0.0 6 112 2 225 113 0.0 6 111 2 226 124 0.0 6 121 2 0 1 227 87 0.0 6 86 1 228 43 0.0 6 42 1 229 34 0.0 6 34 230 50 0.0 6 49 1 231 31 0.0 6 31 232 41 0.0 6 40 1 233 14 0.0 6 13 1 234 14 0.0 6 14 235 14 0.0 6 14 236 3 0.0 6 3 237 14 0.0 6 14 238 4 0.0 6 4 239 11 0.0 6 11 240 8 0.0 6 8 241 5 0.0 6 4 1 242 8 0.0 6 7 1 243 17 0.0 6 17 244 10 0.0 6 9 1 245 8 0.0 6 7 1 246 2 0.0 6 2 247 8 0.0 6 8 248 4 0.0 6 4 249 7 0.0 6 7 250 4 0.0 6 4 251 1 0.0 6 1 252 1 0.0 6 1 253 3 0.0 6 3 254 4 0.0 6 4 255 4 0.0 6 4 256 3 0.0 6 3 280 3 0.0 6 3 281 26 0.0 6 24 2 282 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2232 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 54 1 0.0 6 1 69 4 0.0 6 4 70 18 0.0 6 18 71 5 0.0 6 4 1 72 14 0.0 6 14 73 1 0.0 6 1 74 3 0.0 6 3 75 9 0.0 6 8 1 76 25 0.0 6 25 77 5 0.0 6 5 78 1 0.0 6 1 80 4 0.0 6 4 81 19 0.0 6 19 82 37 0.0 6 37 83 34 0.0 6 34 84 50 0.0 6 50 85 2 0.0 6 1 0 0 0 1 86 11 0.0 6 11 87 5 0.0 6 5 88 32 0.0 6 32 89 17 0.0 6 17 90 4 0.0 6 4 91 24 0.0 6 24 92 27 0.0 6 27 94 10 0.0 6 10 95 35 0.0 6 35 96 8 0.0 6 8 97 1 0.0 6 1 98 7 0.0 6 7 99 40 0.0 6 40 101 2 0.0 6 2 102 1 0.0 6 1 103 1 0.0 6 1 106 1 0.0 6 1 107 11 0.0 6 11 108 2 0.0 6 2 109 4 0.0 6 4 110 46 0.0 6 46 111 1 0.0 6 1 113 10 0.0 6 10 116 3 0.0 6 3 117 12 0.0 6 12 118 55 0.0 6 55 119 2 0.0 6 2 120 1 0.0 6 1 121 16 0.0 6 15 1 122 72 0.0 6 72 123 10 0.0 6 10 124 1 0.0 6 1 125 2 0.0 6 1 0 0 1 126 1 0.0 6 1 132 1 0.0 6 1 133 4 0.0 6 4 134 45 0.0 6 45 135 1 0.0 6 1 136 3 0.0 6 3 137 2 0.0 6 2 138 7 0.0 6 7 139 38 0.0 6 38 140 205 0.0 6 205 141 4 0.0 6 3 1 142 2 0.0 6 2 143 35 0.0 6 34 1 144 114 0.0 6 112 0 0 0 2 145 4 0.0 6 4 146 4 0.0 6 4 147 21 0.0 6 21 148 1 0.0 6 0 0 0 0 1 150 6 0.0 6 6 151 15 0.0 6 15 153 1 0.0 6 1 155 1 0.0 6 1 156 3 0.0 6 3 157 50 0.0 6 50 158 213 0.0 6 212 1 159 8 0.0 6 8 160 5 0.0 6 5 161 49 0.0 6 47 0 0 1 1 162 13 0.0 6 13 163 1 0.0 6 1 164 15 0.0 6 15 165 43 0.0 6 43 169 1 0.0 6 0 0 0 0 1 170 1 0.0 6 1 174 19 0.0 6 19 175 75 0.0 6 75 176 4 0.0 6 3 1 179 7 0.0 6 7 180 36 0.0 6 36 181 33 0.0 6 33 182 6 0.0 6 6 183 5 0.0 6 5 186 1 0.0 6 1 187 7 0.0 6 7 188 3 0.0 6 3 189 13 0.0 6 13 190 1 0.0 6 1 191 1 0.0 6 1 192 1 0.0 6 1 193 1 0.0 6 1 195 1 0.0 6 1 196 6 0.0 6 6 197 52 0.0 6 52 198 258 0.0 6 258 199 7 0.0 6 7 202 1 0.0 6 0 0 0 0 1 207 4 0.0 6 4 208 2 0.0 6 2 218 1 0.0 6 1 219 3 0.0 6 3 220 8 0.0 6 8 221 52 0.0 6 52 250 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_07_206_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_07_433_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_07_206_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_07_433_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.62 s (64 µs/read; 0.94 M reads/minute). === Summary === Total read pairs processed: 88,024 Read 1 with adapter: 88,016 (100.0%) Read 2 with adapter: 87,954 (99.9%) == Read fate breakdown == Pairs that were too short: 1 (0.0%) Pairs discarded as untrimmed: 77 (0.1%) Pairs written (passing filters): 87,946 (99.9%) Total basepairs processed: 43,782,553 bp Read 1: 24,887,139 bp Read 2: 18,895,414 bp Total written (filtered): 5,553,859 bp (12.7%) Read 1: 2,734,420 bp Read 2: 2,819,439 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 87879 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 3 343.8 1 1 2 5 1 86.0 1 1 6 2 21.5 1 0 0 2 9 5 0.3 2 4 1 14 1 0.0 4 1 15 1 0.0 4 0 1 17 2 0.0 5 2 20 21 0.0 6 21 21 8 0.0 6 7 1 22 10 0.0 6 9 1 23 5 0.0 6 4 1 24 2 0.0 6 2 25 9 0.0 6 9 26 7 0.0 6 6 1 27 4 0.0 6 3 0 1 28 7 0.0 6 6 1 29 2 0.0 6 2 30 1 0.0 6 1 33 2 0.0 6 2 34 3 0.0 6 2 1 35 1 0.0 6 1 36 1 0.0 6 1 37 1 0.0 6 1 38 3 0.0 6 2 1 39 2 0.0 6 2 40 3 0.0 6 2 1 41 2 0.0 6 2 42 3 0.0 6 3 43 1 0.0 6 1 44 7 0.0 6 7 45 5 0.0 6 5 46 1 0.0 6 1 47 1 0.0 6 1 49 3 0.0 6 1 1 0 1 50 2 0.0 6 2 51 1 0.0 6 1 52 4 0.0 6 4 53 5 0.0 6 5 56 2 0.0 6 2 57 2 0.0 6 2 58 2 0.0 6 2 59 2 0.0 6 2 60 1 0.0 6 1 61 2 0.0 6 2 62 7 0.0 6 7 63 2 0.0 6 2 64 2 0.0 6 2 65 2 0.0 6 2 66 2 0.0 6 1 1 67 3 0.0 6 3 68 2 0.0 6 2 69 2 0.0 6 2 71 1 0.0 6 1 72 4 0.0 6 3 0 0 0 1 73 3 0.0 6 3 74 2 0.0 6 2 75 1 0.0 6 1 76 1 0.0 6 1 77 5 0.0 6 5 78 1 0.0 6 1 79 2 0.0 6 2 81 2 0.0 6 2 82 1 0.0 6 1 83 3 0.0 6 2 1 84 4 0.0 6 4 85 5 0.0 6 5 86 12 0.0 6 12 87 3 0.0 6 2 1 89 9 0.0 6 9 90 7 0.0 6 7 91 3 0.0 6 1 2 92 2 0.0 6 2 93 6 0.0 6 6 94 1 0.0 6 1 96 2 0.0 6 1 1 97 12 0.0 6 11 1 98 12 0.0 6 11 1 100 8 0.0 6 7 1 101 16 0.0 6 16 102 6 0.0 6 6 103 1 0.0 6 1 104 9 0.0 6 8 1 105 4 0.0 6 4 106 8 0.0 6 8 107 6 0.0 6 6 108 9 0.0 6 9 109 11 0.0 6 11 110 3 0.0 6 3 111 5 0.0 6 4 1 112 10 0.0 6 10 113 9 0.0 6 9 114 12 0.0 6 11 1 115 5 0.0 6 5 116 3 0.0 6 2 1 117 7 0.0 6 7 118 6 0.0 6 6 119 10 0.0 6 10 120 12 0.0 6 11 1 121 11 0.0 6 10 1 122 19 0.0 6 17 2 123 13 0.0 6 12 1 124 7 0.0 6 6 1 125 11 0.0 6 11 126 24 0.0 6 23 1 127 14 0.0 6 13 0 1 128 20 0.0 6 18 1 0 0 0 1 129 22 0.0 6 22 130 12 0.0 6 11 0 0 1 131 15 0.0 6 12 3 132 26 0.0 6 24 1 1 133 12 0.0 6 12 134 37 0.0 6 35 1 1 135 33 0.0 6 31 2 136 22 0.0 6 21 1 137 45 0.0 6 42 3 138 29 0.0 6 27 2 139 28 0.0 6 28 140 31 0.0 6 30 1 141 52 0.0 6 51 1 142 26 0.0 6 25 1 143 60 0.0 6 58 2 144 127 0.0 6 124 2 0 1 145 151 0.0 6 149 2 146 157 0.0 6 156 1 147 149 0.0 6 146 3 148 130 0.0 6 129 1 149 144 0.0 6 143 1 150 172 0.0 6 172 151 140 0.0 6 138 2 152 118 0.0 6 118 153 158 0.0 6 156 2 154 106 0.0 6 106 155 80 0.0 6 78 2 156 70 0.0 6 69 1 157 96 0.0 6 95 1 158 65 0.0 6 65 159 70 0.0 6 70 160 74 0.0 6 71 3 161 93 0.0 6 92 1 162 108 0.0 6 108 163 93 0.0 6 93 164 106 0.0 6 104 2 165 94 0.0 6 93 1 166 107 0.0 6 106 1 167 65 0.0 6 64 1 168 80 0.0 6 80 169 120 0.0 6 116 4 170 121 0.0 6 120 1 171 93 0.0 6 93 172 161 0.0 6 159 2 173 121 0.0 6 119 2 174 142 0.0 6 141 1 175 99 0.0 6 97 2 176 98 0.0 6 97 1 177 99 0.0 6 95 4 178 65 0.0 6 64 1 179 92 0.0 6 90 2 180 138 0.0 6 134 4 181 259 0.0 6 257 2 182 187 0.0 6 181 6 183 129 0.0 6 124 5 184 167 0.0 6 165 2 185 180 0.0 6 175 4 1 186 206 0.0 6 200 6 187 189 0.0 6 181 8 188 223 0.0 6 219 4 189 177 0.0 6 173 4 190 206 0.0 6 200 6 191 186 0.0 6 184 2 192 181 0.0 6 175 5 1 193 219 0.0 6 213 6 194 203 0.0 6 198 5 195 264 0.0 6 260 4 196 356 0.0 6 351 5 197 316 0.0 6 307 7 1 1 198 379 0.0 6 371 8 199 315 0.0 6 307 7 1 200 367 0.0 6 358 9 201 319 0.0 6 313 5 1 202 338 0.0 6 328 10 203 237 0.0 6 227 10 204 340 0.0 6 335 5 205 367 0.0 6 359 8 206 365 0.0 6 359 6 207 371 0.0 6 360 11 208 246 0.0 6 241 5 209 305 0.0 6 297 8 210 222 0.0 6 218 4 211 235 0.0 6 230 5 212 236 0.0 6 231 4 1 213 320 0.0 6 316 4 214 502 0.0 6 496 6 215 391 0.0 6 383 8 216 405 0.0 6 398 7 217 344 0.0 6 340 4 218 322 0.0 6 318 4 219 391 0.0 6 384 7 220 331 0.0 6 328 3 221 250 0.0 6 246 4 222 405 0.0 6 391 13 1 223 536 0.0 6 519 17 224 612 0.0 6 597 15 225 478 0.0 6 462 16 226 537 0.0 6 526 10 1 227 393 0.0 6 389 4 228 308 0.0 6 305 3 229 326 0.0 6 321 5 230 339 0.0 6 331 8 231 338 0.0 6 329 9 232 301 0.0 6 295 6 233 300 0.0 6 289 11 234 308 0.0 6 301 6 1 235 283 0.0 6 277 4 0 2 236 325 0.0 6 316 9 237 524 0.0 6 514 9 1 238 609 0.0 6 592 16 1 239 673 0.0 6 656 16 1 240 653 0.0 6 638 15 241 613 0.0 6 601 12 242 651 0.0 6 636 14 1 243 535 0.0 6 527 7 1 244 437 0.0 6 428 9 245 438 0.0 6 430 7 0 1 246 513 0.0 6 505 8 247 479 0.0 6 474 5 248 460 0.0 6 449 9 2 249 520 0.0 6 511 9 250 545 0.0 6 530 15 251 661 0.0 6 646 15 252 688 0.0 6 667 19 2 253 389 0.0 6 382 6 0 1 254 387 0.0 6 379 8 255 506 0.0 6 496 10 256 706 0.0 6 689 17 257 736 0.0 6 718 17 0 1 258 687 0.0 6 663 23 0 1 259 860 0.0 6 842 17 0 1 260 1134 0.0 6 1115 18 1 261 1033 0.0 6 1015 16 2 262 486 0.0 6 474 12 263 594 0.0 6 580 13 1 264 429 0.0 6 425 4 265 413 0.0 6 400 12 1 266 656 0.0 6 645 11 267 672 0.0 6 656 15 1 268 567 0.0 6 550 17 269 1307 0.0 6 1275 29 3 270 1359 0.0 6 1330 28 1 271 740 0.0 6 723 16 0 1 272 734 0.0 6 714 19 1 273 1010 0.0 6 978 31 0 1 274 1615 0.0 6 1589 25 1 275 1328 0.0 6 1313 14 1 276 1632 0.0 6 1589 37 3 0 2 0 1 277 1892 0.0 6 1850 41 1 278 3505 0.0 6 3421 82 2 279 22731 0.0 6 22267 459 4 0 1 280 8273 0.0 6 8060 212 0 1 281 48 0.0 6 40 6 1 1 282 2 0.0 6 1 0 0 1 283 2 0.0 6 1 0 0 0 1 285 1 0.0 6 1 287 1 0.0 6 0 0 0 0 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 137 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 10 0.0 6 10 72 3 0.0 6 3 73 2 0.0 6 2 74 2 0.0 6 2 75 2 0.0 6 2 76 2 0.0 6 2 77 2 0.0 6 2 78 2 0.0 6 2 79 6 0.0 6 6 80 5 0.0 6 5 81 1 0.0 6 1 83 1 0.0 6 1 84 2 0.0 6 2 86 3 0.0 6 3 88 1 0.0 6 1 91 2 0.0 6 2 92 2 0.0 6 2 93 1 0.0 6 1 94 2 0.0 6 2 95 2 0.0 6 2 96 2 0.0 6 2 97 2 0.0 6 2 98 1 0.0 6 1 100 2 0.0 6 2 102 3 0.0 6 3 103 1 0.0 6 1 104 1 0.0 6 1 106 1 0.0 6 1 107 1 0.0 6 1 108 4 0.0 6 4 109 4 0.0 6 4 110 1 0.0 6 1 111 1 0.0 6 1 112 2 0.0 6 2 113 1 0.0 6 1 114 1 0.0 6 1 119 1 0.0 6 1 120 1 0.0 6 1 121 1 0.0 6 1 123 1 0.0 6 1 124 1 0.0 6 1 125 1 0.0 6 1 126 1 0.0 6 1 127 1 0.0 6 1 128 1 0.0 6 1 130 3 0.0 6 3 131 1 0.0 6 1 133 1 0.0 6 1 134 1 0.0 6 1 138 1 0.0 6 1 141 1 0.0 6 1 143 1 0.0 6 1 144 1 0.0 6 1 147 2 0.0 6 2 148 1 0.0 6 1 149 1 0.0 6 1 150 1 0.0 6 1 151 2 0.0 6 2 154 1 0.0 6 1 155 1 0.0 6 1 157 2 0.0 6 2 158 1 0.0 6 1 162 2 0.0 6 2 163 1 0.0 6 1 166 1 0.0 6 1 167 1 0.0 6 1 168 2 0.0 6 2 170 1 0.0 6 1 171 1 0.0 6 1 172 2 0.0 6 2 173 1 0.0 6 1 177 1 0.0 6 1 179 1 0.0 6 1 183 1 0.0 6 1 184 1 0.0 6 1 186 1 0.0 6 1 187 2 0.0 6 2 192 1 0.0 6 1 195 2 0.0 6 2 229 1 0.0 6 1 236 1 0.0 6 1 257 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 87795 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 21.5 1 1 7 1 5.4 2 0 0 1 10 4 0.1 3 4 11 4 0.0 3 4 12 3 0.0 3 3 13 12 0.0 3 11 1 14 12 0.0 4 12 15 1 0.0 4 1 16 14 0.0 4 14 17 24 0.0 5 22 2 18 19 0.0 5 17 2 19 14 0.0 5 12 2 20 81 0.0 6 77 4 21 80 0.0 6 75 5 22 37 0.0 6 34 3 23 29 0.0 6 29 24 18 0.0 6 17 1 25 25 0.0 6 23 2 26 25 0.0 6 24 1 27 21 0.0 6 20 1 28 20 0.0 6 19 0 1 29 24 0.0 6 21 3 30 14 0.0 6 13 1 31 11 0.0 6 10 0 0 1 32 12 0.0 6 11 0 1 33 15 0.0 6 14 1 34 18 0.0 6 16 2 35 5 0.0 6 5 36 8 0.0 6 4 3 1 37 11 0.0 6 10 1 38 6 0.0 6 5 1 39 9 0.0 6 9 40 8 0.0 6 8 41 14 0.0 6 13 0 1 42 8 0.0 6 8 43 19 0.0 6 17 2 44 18 0.0 6 18 45 12 0.0 6 10 2 46 22 0.0 6 21 1 47 28 0.0 6 24 4 48 17 0.0 6 14 3 49 23 0.0 6 21 0 2 50 17 0.0 6 14 3 51 11 0.0 6 10 1 52 40 0.0 6 35 4 1 53 25 0.0 6 21 4 54 32 0.0 6 28 3 1 55 21 0.0 6 19 2 56 14 0.0 6 13 0 1 57 21 0.0 6 19 2 58 23 0.0 6 22 1 59 18 0.0 6 18 60 24 0.0 6 22 2 61 12 0.0 6 12 62 14 0.0 6 13 0 1 63 14 0.0 6 13 1 64 22 0.0 6 20 2 65 12 0.0 6 10 2 66 34 0.0 6 26 6 1 1 67 158 0.0 6 152 6 68 49 0.0 6 46 3 69 79 0.0 6 74 5 70 22 0.0 6 19 3 71 42 0.0 6 35 6 1 72 34 0.0 6 34 73 13 0.0 6 11 2 74 16 0.0 6 14 2 75 18 0.0 6 18 76 39 0.0 6 37 2 77 28 0.0 6 24 3 0 0 0 0 1 78 42 0.0 6 38 4 79 28 0.0 6 25 3 80 25 0.0 6 20 5 81 25 0.0 6 23 1 1 82 53 0.0 6 51 2 83 71 0.0 6 67 4 84 61 0.0 6 58 2 0 1 85 27 0.0 6 25 1 1 86 27 0.0 6 25 1 0 0 0 1 87 68 0.0 6 59 6 0 1 2 88 38 0.0 6 37 1 89 44 0.0 6 40 4 90 41 0.0 6 36 5 91 49 0.0 6 46 3 92 31 0.0 6 28 2 1 93 67 0.0 6 63 3 0 0 1 94 99 0.0 6 91 8 95 54 0.0 6 47 7 96 55 0.0 6 54 0 0 0 1 97 53 0.0 6 50 3 98 49 0.0 6 44 4 0 0 1 99 60 0.0 6 54 5 1 100 59 0.0 6 56 2 0 0 1 101 62 0.0 6 59 3 102 106 0.0 6 100 5 0 0 1 103 111 0.0 6 99 12 104 113 0.0 6 109 4 105 108 0.0 6 102 5 1 106 52 0.0 6 47 5 107 73 0.0 6 68 4 1 108 74 0.0 6 70 4 109 75 0.0 6 74 0 1 110 84 0.0 6 81 3 111 86 0.0 6 83 3 112 90 0.0 6 85 5 113 86 0.0 6 84 2 114 121 0.0 6 114 7 115 97 0.0 6 92 5 116 89 0.0 6 87 1 0 0 1 117 76 0.0 6 71 5 118 97 0.0 6 92 5 119 99 0.0 6 92 7 120 119 0.0 6 114 5 121 96 0.0 6 90 5 0 1 122 112 0.0 6 104 5 2 1 123 114 0.0 6 110 3 0 0 1 124 134 0.0 6 133 1 125 138 0.0 6 133 5 126 122 0.0 6 119 3 127 148 0.0 6 139 9 128 93 0.0 6 88 5 129 115 0.0 6 112 3 130 119 0.0 6 118 0 1 131 170 0.0 6 164 5 1 132 200 0.0 6 188 11 1 133 169 0.0 6 164 5 134 280 0.0 6 272 8 135 419 0.0 6 407 12 136 215 0.0 6 210 5 137 259 0.0 6 247 9 2 0 0 1 138 179 0.0 6 171 7 0 0 0 0 1 139 292 0.0 6 279 11 1 0 0 1 140 456 0.0 6 444 10 1 1 141 406 0.0 6 389 16 1 142 555 0.0 6 536 18 0 0 1 143 445 0.0 6 425 19 0 1 144 607 0.0 6 594 13 145 388 0.0 6 375 13 146 395 0.0 6 387 8 147 321 0.0 6 313 6 1 0 1 148 403 0.0 6 389 13 0 1 149 618 0.0 6 598 20 150 958 0.0 6 925 32 1 151 365 0.0 6 348 14 1 1 1 152 834 0.0 6 802 28 2 0 2 153 530 0.0 6 511 16 0 1 2 154 521 0.0 6 507 14 155 528 0.0 6 513 15 156 502 0.0 6 489 11 1 1 157 678 0.0 6 662 15 1 158 669 0.0 6 652 16 1 159 373 0.0 6 363 10 160 611 0.0 6 598 11 0 1 1 161 343 0.0 6 332 11 162 331 0.0 6 321 10 163 576 0.0 6 550 24 2 164 489 0.0 6 472 16 1 165 601 0.0 6 585 14 0 2 166 687 0.0 6 667 20 167 426 0.0 6 417 8 1 168 896 0.0 6 866 28 0 1 0 1 169 375 0.0 6 359 13 1 0 2 170 406 0.0 6 394 12 171 761 0.0 6 742 19 172 448 0.0 6 434 13 0 1 173 714 0.0 6 692 19 3 174 901 0.0 6 871 27 0 1 2 175 1515 0.0 6 1466 46 1 1 1 176 937 0.0 6 908 28 0 1 177 834 0.0 6 809 22 0 0 2 1 178 706 0.0 6 686 19 0 0 1 179 713 0.0 6 695 13 2 1 2 180 640 0.0 6 615 23 1 1 181 875 0.0 6 847 27 0 0 1 182 229 0.0 6 214 12 3 183 884 0.0 6 860 20 2 0 2 184 729 0.0 6 704 22 3 185 974 0.0 6 940 32 1 0 1 186 1800 0.0 6 1747 49 3 0 1 187 1546 0.0 6 1504 42 188 1532 0.0 6 1498 30 2 0 2 189 1282 0.0 6 1226 55 1 190 2118 0.0 6 2059 59 191 2050 0.0 6 2000 49 0 0 1 192 1109 0.0 6 1077 32 193 745 0.0 6 719 25 0 0 1 194 1395 0.0 6 1347 45 1 0 2 195 2209 0.0 6 2131 67 2 3 6 196 3111 0.0 6 3029 76 3 1 2 197 1299 0.0 6 1249 45 3 1 0 1 198 2684 0.0 6 2613 70 0 0 0 1 199 3042 0.0 6 2962 77 1 0 2 200 2010 0.0 6 1949 58 0 0 2 0 1 201 1895 0.0 6 1844 48 2 0 1 202 1023 0.0 6 1000 23 203 1019 0.0 6 995 23 1 204 1071 0.0 6 1046 25 205 551 0.0 6 539 11 0 1 206 440 0.0 6 420 18 1 0 1 207 413 0.0 6 400 13 208 658 0.0 6 636 22 209 416 0.0 6 407 8 0 0 1 210 372 0.0 6 357 14 1 211 475 0.0 6 463 11 0 0 1 212 464 0.0 6 450 12 1 0 1 213 293 0.0 6 284 8 1 214 957 0.0 6 937 19 1 215 839 0.0 6 817 19 1 1 1 216 951 0.0 6 927 24 217 695 0.0 6 669 22 0 1 3 218 657 0.0 6 643 13 0 0 1 219 706 0.0 6 688 18 220 358 0.0 6 347 11 221 845 0.0 6 826 17 1 0 1 222 646 0.0 6 629 16 0 0 1 223 903 0.0 6 877 24 1 1 224 561 0.0 6 542 17 1 0 1 225 532 0.0 6 521 11 226 460 0.0 6 447 12 0 0 1 227 483 0.0 6 472 10 0 0 1 228 372 0.0 6 358 13 0 0 1 229 503 0.0 6 486 15 0 1 0 0 1 230 556 0.0 6 537 19 231 490 0.0 6 474 15 0 1 232 631 0.0 6 622 8 1 233 288 0.0 6 282 5 1 234 229 0.0 6 222 6 1 235 126 0.0 6 122 4 236 55 0.0 6 53 2 237 41 0.0 6 41 238 107 0.0 6 106 1 239 77 0.0 6 77 240 131 0.0 6 126 5 241 282 0.0 6 273 9 242 223 0.0 6 217 6 243 191 0.0 6 178 11 1 0 1 244 140 0.0 6 135 5 245 123 0.0 6 119 4 246 134 0.0 6 132 2 247 73 0.0 6 68 4 1 248 111 0.0 6 107 4 249 29 0.0 6 27 2 250 23 0.0 6 23 251 65 0.0 6 63 2 252 11 0.0 6 11 253 5 0.0 6 5 254 4 0.0 6 4 255 2 0.0 6 2 258 1 0.0 6 1 259 1 0.0 6 1 263 1 0.0 6 1 277 3 0.0 6 2 0 1 278 27 0.0 6 26 1 279 55 0.0 6 54 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 159 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 68 2 0.0 6 2 73 4 0.0 6 4 74 8 0.0 6 8 75 2 0.0 6 2 76 4 0.0 6 4 77 1 0.0 6 1 78 2 0.0 6 2 79 5 0.0 6 5 80 1 0.0 6 1 81 2 0.0 6 2 82 6 0.0 6 6 83 3 0.0 6 3 85 3 0.0 6 3 87 1 0.0 6 1 88 2 0.0 6 2 89 1 0.0 6 1 91 1 0.0 6 1 92 3 0.0 6 3 93 1 0.0 6 1 95 2 0.0 6 2 97 2 0.0 6 2 98 2 0.0 6 2 101 4 0.0 6 4 103 1 0.0 6 1 104 1 0.0 6 1 105 1 0.0 6 1 108 1 0.0 6 1 109 2 0.0 6 2 110 3 0.0 6 3 111 2 0.0 6 2 112 1 0.0 6 1 113 5 0.0 6 5 115 1 0.0 6 1 116 2 0.0 6 2 117 2 0.0 6 2 118 2 0.0 6 2 119 3 0.0 6 3 120 1 0.0 6 1 121 1 0.0 6 1 123 1 0.0 6 1 124 1 0.0 6 1 125 1 0.0 6 1 128 11 0.0 6 11 129 3 0.0 6 3 130 1 0.0 6 1 132 1 0.0 6 1 133 1 0.0 6 1 134 1 0.0 6 1 135 2 0.0 6 2 136 1 0.0 6 1 138 3 0.0 6 3 139 3 0.0 6 3 140 3 0.0 6 3 142 1 0.0 6 1 143 3 0.0 6 3 144 1 0.0 6 1 145 2 0.0 6 2 146 1 0.0 6 1 147 2 0.0 6 2 149 1 0.0 6 1 151 2 0.0 6 2 152 1 0.0 6 1 156 1 0.0 6 1 158 1 0.0 6 1 164 2 0.0 6 2 166 1 0.0 6 1 167 3 0.0 6 3 168 1 0.0 6 1 169 1 0.0 6 1 170 1 0.0 6 1 171 1 0.0 6 1 172 2 0.0 6 2 177 1 0.0 6 1 178 1 0.0 6 1 181 1 0.0 6 1 184 1 0.0 6 1 188 1 0.0 6 1 191 1 0.0 6 1 210 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_08_207_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_08_434_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_08_207_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_08_434_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.98 s (77 µs/read; 0.78 M reads/minute). === Summary === Total read pairs processed: 64,436 Read 1 with adapter: 64,432 (100.0%) Read 2 with adapter: 64,436 (100.0%) == Read fate breakdown == Pairs that were too short: 13 (0.0%) Pairs discarded as untrimmed: 4 (0.0%) Pairs written (passing filters): 64,419 (100.0%) Total basepairs processed: 29,161,960 bp Read 1: 16,505,205 bp Read 2: 12,656,755 bp Total written (filtered): 4,081,605 bp (14.0%) Read 1: 2,055,374 bp Read 2: 2,026,231 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 63325 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1006.8 0 1 4 1 251.7 1 1 6 4 15.7 1 0 0 4 7 1 3.9 2 0 0 1 9 1 0.2 2 1 16 1 0.0 4 1 17 1 0.0 5 1 18 2 0.0 5 2 19 2 0.0 5 2 20 56 0.0 6 56 21 32 0.0 6 28 4 22 32 0.0 6 31 1 23 16 0.0 6 14 1 0 1 24 5 0.0 6 5 25 13 0.0 6 13 26 12 0.0 6 12 27 6 0.0 6 4 1 0 0 1 28 5 0.0 6 5 29 5 0.0 6 5 30 4 0.0 6 4 31 5 0.0 6 4 1 32 4 0.0 6 4 33 1 0.0 6 1 34 6 0.0 6 6 35 1 0.0 6 1 36 5 0.0 6 5 38 2 0.0 6 2 39 2 0.0 6 2 41 7 0.0 6 7 42 5 0.0 6 5 43 3 0.0 6 3 44 3 0.0 6 3 45 1 0.0 6 1 46 1 0.0 6 1 47 2 0.0 6 2 48 7 0.0 6 5 1 1 49 3 0.0 6 3 50 3 0.0 6 3 51 4 0.0 6 3 1 52 4 0.0 6 3 1 53 4 0.0 6 4 54 4 0.0 6 4 55 2 0.0 6 2 56 3 0.0 6 3 57 6 0.0 6 5 1 58 7 0.0 6 7 59 2 0.0 6 2 60 4 0.0 6 2 2 61 3 0.0 6 3 62 4 0.0 6 4 63 2 0.0 6 1 1 64 5 0.0 6 5 65 4 0.0 6 4 66 4 0.0 6 4 67 5 0.0 6 5 68 4 0.0 6 4 69 2 0.0 6 2 70 3 0.0 6 3 71 5 0.0 6 5 72 3 0.0 6 3 73 5 0.0 6 4 0 0 0 1 74 3 0.0 6 3 75 8 0.0 6 8 76 6 0.0 6 6 77 8 0.0 6 8 78 4 0.0 6 3 1 79 2 0.0 6 2 80 2 0.0 6 2 81 7 0.0 6 7 82 3 0.0 6 3 83 4 0.0 6 4 84 10 0.0 6 9 1 85 9 0.0 6 9 86 12 0.0 6 12 87 8 0.0 6 8 88 5 0.0 6 5 89 7 0.0 6 7 90 5 0.0 6 5 91 5 0.0 6 5 92 3 0.0 6 3 93 3 0.0 6 3 94 5 0.0 6 4 0 0 0 1 95 2 0.0 6 2 96 7 0.0 6 7 97 4 0.0 6 4 98 8 0.0 6 8 99 4 0.0 6 2 1 1 100 10 0.0 6 8 2 101 14 0.0 6 13 1 102 10 0.0 6 9 1 103 7 0.0 6 6 1 104 6 0.0 6 6 105 13 0.0 6 12 1 106 9 0.0 6 9 107 18 0.0 6 17 1 108 8 0.0 6 8 109 6 0.0 6 5 1 110 12 0.0 6 12 111 15 0.0 6 14 1 112 7 0.0 6 6 0 1 113 13 0.0 6 13 114 13 0.0 6 13 115 8 0.0 6 8 116 12 0.0 6 12 117 12 0.0 6 12 118 14 0.0 6 14 119 10 0.0 6 10 120 30 0.0 6 30 121 11 0.0 6 11 122 16 0.0 6 15 0 1 123 24 0.0 6 24 124 23 0.0 6 23 125 23 0.0 6 23 126 15 0.0 6 15 127 17 0.0 6 14 3 128 18 0.0 6 17 1 129 26 0.0 6 26 130 23 0.0 6 23 131 24 0.0 6 24 132 16 0.0 6 15 1 133 18 0.0 6 18 134 22 0.0 6 21 0 1 135 23 0.0 6 22 1 136 21 0.0 6 21 137 35 0.0 6 35 138 41 0.0 6 41 139 33 0.0 6 32 1 140 32 0.0 6 32 141 40 0.0 6 40 142 92 0.0 6 90 2 143 167 0.0 6 167 144 318 0.0 6 316 2 145 137 0.0 6 137 146 194 0.0 6 194 147 228 0.0 6 227 1 148 196 0.0 6 195 1 149 174 0.0 6 170 4 150 220 0.0 6 219 1 151 324 0.0 6 324 152 277 0.0 6 276 1 153 188 0.0 6 188 154 150 0.0 6 150 155 207 0.0 6 206 1 156 257 0.0 6 257 157 111 0.0 6 111 158 227 0.0 6 227 159 234 0.0 6 231 3 160 147 0.0 6 147 161 204 0.0 6 203 1 162 241 0.0 6 236 5 163 351 0.0 6 350 1 164 233 0.0 6 232 1 165 410 0.0 6 409 1 166 364 0.0 6 363 1 167 221 0.0 6 221 168 144 0.0 6 142 2 169 145 0.0 6 145 170 322 0.0 6 321 1 171 297 0.0 6 295 1 0 1 172 338 0.0 6 337 1 173 209 0.0 6 209 174 172 0.0 6 171 1 175 212 0.0 6 210 2 176 516 0.0 6 515 1 177 336 0.0 6 332 3 1 178 331 0.0 6 331 179 154 0.0 6 154 180 370 0.0 6 369 1 181 172 0.0 6 171 1 182 201 0.0 6 200 0 0 0 0 1 183 189 0.0 6 188 1 184 194 0.0 6 192 2 185 422 0.0 6 420 2 186 635 0.0 6 633 2 187 307 0.0 6 306 1 188 402 0.0 6 399 3 189 469 0.0 6 466 3 190 311 0.0 6 308 2 1 191 611 0.0 6 609 1 0 0 0 0 1 192 317 0.0 6 317 193 587 0.0 6 583 3 1 194 316 0.0 6 309 7 195 402 0.0 6 397 3 0 0 1 1 196 344 0.0 6 338 6 197 482 0.0 6 477 5 198 626 0.0 6 621 4 1 199 393 0.0 6 389 3 1 200 340 0.0 6 337 2 1 201 325 0.0 6 324 1 202 354 0.0 6 348 6 203 434 0.0 6 422 12 204 485 0.0 6 484 1 205 588 0.0 6 583 5 206 419 0.0 6 418 1 207 703 0.0 6 698 5 208 554 0.0 6 550 2 2 209 644 0.0 6 641 3 210 610 0.0 6 606 3 0 0 0 0 1 211 495 0.0 6 492 3 212 462 0.0 6 459 3 213 623 0.0 6 620 3 214 593 0.0 6 588 4 1 215 699 0.0 6 695 3 0 0 0 1 216 780 0.0 6 773 6 0 1 217 595 0.0 6 593 1 1 218 552 0.0 6 547 3 1 0 0 1 219 547 0.0 6 541 6 220 474 0.0 6 470 4 221 385 0.0 6 383 2 222 493 0.0 6 487 6 223 405 0.0 6 401 3 1 224 452 0.0 6 448 4 225 564 0.0 6 560 4 226 722 0.0 6 715 6 1 227 592 0.0 6 586 5 0 1 228 567 0.0 6 560 6 0 0 0 1 229 357 0.0 6 351 6 230 326 0.0 6 325 1 231 391 0.0 6 386 5 232 334 0.0 6 329 4 1 233 365 0.0 6 361 3 0 0 0 0 1 234 393 0.0 6 388 5 235 314 0.0 6 311 3 236 332 0.0 6 327 5 237 358 0.0 6 352 6 238 288 0.0 6 281 7 239 352 0.0 6 348 4 240 402 0.0 6 394 8 241 472 0.0 6 462 10 242 398 0.0 6 388 10 243 428 0.0 6 422 4 2 244 374 0.0 6 369 5 245 444 0.0 6 438 6 246 290 0.0 6 288 2 247 277 0.0 6 273 4 248 262 0.0 6 259 3 249 409 0.0 6 408 1 250 250 0.0 6 243 7 251 275 0.0 6 273 2 252 288 0.0 6 281 7 253 203 0.0 6 200 3 254 337 0.0 6 330 6 1 255 252 0.0 6 251 1 256 236 0.0 6 232 4 257 253 0.0 6 244 8 0 0 0 0 1 258 258 0.0 6 250 5 1 1 0 0 1 259 334 0.0 6 326 7 1 260 429 0.0 6 425 4 261 206 0.0 6 202 2 1 1 262 201 0.0 6 198 3 263 462 0.0 6 460 2 264 200 0.0 6 193 7 265 236 0.0 6 229 7 266 159 0.0 6 150 7 1 0 0 1 267 212 0.0 6 210 2 268 188 0.0 6 184 4 269 318 0.0 6 310 6 1 0 0 1 270 219 0.0 6 218 1 271 273 0.0 6 266 7 272 462 0.0 6 445 17 273 280 0.0 6 274 6 274 378 0.0 6 363 14 0 1 275 401 0.0 6 396 5 276 587 0.0 6 571 15 0 1 277 806 0.0 6 784 21 1 278 608 0.0 6 587 21 279 1561 0.0 6 1530 27 2 2 280 11321 0.0 6 10997 318 5 0 1 281 66 0.0 6 58 8 282 1 0.0 6 1 283 1 0.0 6 1 284 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1107 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 61 1 0.0 6 0 1 62 51 0.0 6 49 2 71 5 0.0 6 5 72 3 0.0 6 3 73 8 0.0 6 8 74 8 0.0 6 8 75 7 0.0 6 7 76 10 0.0 6 10 77 3 0.0 6 3 78 4 0.0 6 4 79 10 0.0 6 10 80 13 0.0 6 13 81 4 0.0 6 4 82 3 0.0 6 3 83 3 0.0 6 3 84 5 0.0 6 5 85 5 0.0 6 5 86 4 0.0 6 4 87 3 0.0 6 3 88 6 0.0 6 6 89 3 0.0 6 3 90 6 0.0 6 6 91 9 0.0 6 9 92 16 0.0 6 16 93 7 0.0 6 7 94 8 0.0 6 8 95 8 0.0 6 7 1 96 7 0.0 6 7 98 6 0.0 6 6 99 9 0.0 6 9 100 13 0.0 6 13 101 10 0.0 6 10 102 6 0.0 6 6 103 2 0.0 6 2 104 10 0.0 6 10 105 16 0.0 6 16 106 8 0.0 6 8 107 7 0.0 6 7 108 7 0.0 6 7 109 8 0.0 6 8 110 8 0.0 6 8 111 2 0.0 6 2 112 1 0.0 6 1 113 3 0.0 6 3 114 16 0.0 6 16 115 16 0.0 6 16 116 2 0.0 6 2 117 8 0.0 6 8 118 14 0.0 6 14 119 11 0.0 6 11 120 20 0.0 6 20 121 9 0.0 6 9 122 15 0.0 6 15 123 4 0.0 6 4 124 1 0.0 6 1 125 3 0.0 6 3 126 14 0.0 6 14 127 13 0.0 6 13 128 2 0.0 6 2 129 1 0.0 6 1 130 5 0.0 6 5 131 2 0.0 6 2 132 3 0.0 6 3 133 2 0.0 6 2 134 19 0.0 6 19 135 1 0.0 6 1 136 7 0.0 6 7 137 3 0.0 6 3 138 11 0.0 6 11 139 8 0.0 6 8 140 12 0.0 6 12 141 6 0.0 6 6 142 6 0.0 6 6 143 10 0.0 6 9 1 144 6 0.0 6 6 145 8 0.0 6 8 146 5 0.0 6 5 147 7 0.0 6 7 148 9 0.0 6 9 149 5 0.0 6 5 150 10 0.0 6 10 151 7 0.0 6 7 152 2 0.0 6 2 153 4 0.0 6 4 154 6 0.0 6 6 156 6 0.0 6 6 157 13 0.0 6 13 158 5 0.0 6 5 159 3 0.0 6 3 160 5 0.0 6 5 162 1 0.0 6 1 163 2 0.0 6 2 164 5 0.0 6 5 165 7 0.0 6 7 166 10 0.0 6 10 167 4 0.0 6 4 168 3 0.0 6 3 169 9 0.0 6 9 170 3 0.0 6 3 171 6 0.0 6 6 172 10 0.0 6 10 173 5 0.0 6 5 174 8 0.0 6 8 175 6 0.0 6 6 176 3 0.0 6 3 177 10 0.0 6 10 178 26 0.0 6 26 179 5 0.0 6 5 180 1 0.0 6 1 181 2 0.0 6 2 182 6 0.0 6 6 183 3 0.0 6 3 184 20 0.0 6 20 185 5 0.0 6 5 186 9 0.0 6 9 187 14 0.0 6 14 188 18 0.0 6 18 189 5 0.0 6 5 190 12 0.0 6 12 191 2 0.0 6 2 192 3 0.0 6 3 193 5 0.0 6 5 194 4 0.0 6 4 196 6 0.0 6 6 198 1 0.0 6 1 199 1 0.0 6 1 200 2 0.0 6 2 201 3 0.0 6 3 202 4 0.0 6 4 203 3 0.0 6 3 204 2 0.0 6 2 205 4 0.0 6 4 206 6 0.0 6 6 207 4 0.0 6 4 208 5 0.0 6 5 209 5 0.0 6 4 0 0 0 0 0 1 211 13 0.0 6 13 212 4 0.0 6 4 213 1 0.0 6 1 214 5 0.0 6 5 215 5 0.0 6 5 216 2 0.0 6 2 217 3 0.0 6 3 218 4 0.0 6 4 219 1 0.0 6 1 220 3 0.0 6 3 221 6 0.0 6 6 222 9 0.0 6 9 223 3 0.0 6 3 224 1 0.0 6 1 225 5 0.0 6 5 226 2 0.0 6 2 228 3 0.0 6 3 230 1 0.0 6 1 231 4 0.0 6 4 232 3 0.0 6 3 233 4 0.0 6 4 234 1 0.0 6 1 235 2 0.0 6 2 237 4 0.0 6 4 238 4 0.0 6 4 239 2 0.0 6 2 240 2 0.0 6 2 241 4 0.0 6 4 242 1 0.0 6 1 243 3 0.0 6 3 244 1 0.0 6 1 245 3 0.0 6 3 249 4 0.0 6 4 250 1 0.0 6 1 251 4 0.0 6 4 252 6 0.0 6 6 253 1 0.0 6 1 254 2 0.0 6 2 255 1 0.0 6 1 256 1 0.0 6 1 257 1 0.0 6 1 259 1 0.0 6 1 260 1 0.0 6 1 262 1 0.0 6 1 264 1 0.0 6 1 265 1 0.0 6 1 266 2 0.0 6 2 268 1 0.0 6 1 272 1 0.0 6 1 276 1 0.0 6 1 277 2 0.0 6 2 278 1 0.0 6 1 279 2 0.0 6 2 283 1 0.0 6 1 284 1 0.0 6 1 288 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 63371 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 1 15.7 1 1 7 2 3.9 2 1 1 8 1 1.0 2 1 10 4 0.1 3 4 11 7 0.0 3 7 12 10 0.0 3 9 1 13 8 0.0 3 8 14 18 0.0 4 17 1 15 2 0.0 4 2 16 4 0.0 4 4 17 25 0.0 5 21 4 18 16 0.0 5 14 2 19 10 0.0 5 10 20 48 0.0 6 47 1 21 52 0.0 6 48 3 0 1 22 31 0.0 6 31 23 28 0.0 6 24 4 24 21 0.0 6 21 25 14 0.0 6 14 26 17 0.0 6 16 1 27 14 0.0 6 12 1 1 28 11 0.0 6 10 1 29 17 0.0 6 14 3 30 13 0.0 6 13 31 6 0.0 6 5 1 32 11 0.0 6 10 1 33 11 0.0 6 10 1 34 11 0.0 6 11 35 8 0.0 6 7 1 36 9 0.0 6 8 1 37 9 0.0 6 9 38 19 0.0 6 19 39 10 0.0 6 7 3 40 12 0.0 6 12 41 6 0.0 6 5 1 42 9 0.0 6 9 43 7 0.0 6 5 2 44 17 0.0 6 16 1 45 16 0.0 6 14 2 46 12 0.0 6 12 47 21 0.0 6 19 2 48 19 0.0 6 17 2 49 20 0.0 6 19 1 50 16 0.0 6 14 1 0 1 51 12 0.0 6 11 1 52 33 0.0 6 30 3 53 23 0.0 6 21 2 54 25 0.0 6 20 4 1 55 20 0.0 6 20 56 13 0.0 6 13 57 25 0.0 6 25 58 20 0.0 6 19 1 59 15 0.0 6 13 2 60 19 0.0 6 17 2 61 18 0.0 6 17 1 62 9 0.0 6 7 2 63 20 0.0 6 17 2 1 64 24 0.0 6 22 2 65 17 0.0 6 14 2 1 66 28 0.0 6 27 1 67 44 0.0 6 40 4 68 119 0.0 6 112 5 1 1 69 37 0.0 6 36 1 70 44 0.0 6 44 71 36 0.0 6 32 4 72 49 0.0 6 47 2 73 19 0.0 6 17 2 74 21 0.0 6 21 75 25 0.0 6 20 5 76 26 0.0 6 22 3 1 77 35 0.0 6 31 4 78 35 0.0 6 34 1 79 28 0.0 6 26 2 80 34 0.0 6 31 3 81 37 0.0 6 33 4 82 36 0.0 6 32 3 1 83 51 0.0 6 49 1 0 0 1 84 63 0.0 6 59 4 85 40 0.0 6 36 1 2 0 1 86 35 0.0 6 32 2 1 87 47 0.0 6 46 1 88 33 0.0 6 29 4 89 51 0.0 6 47 3 0 1 90 58 0.0 6 53 5 91 55 0.0 6 50 4 1 92 48 0.0 6 46 2 93 65 0.0 6 59 5 1 94 96 0.0 6 85 11 95 56 0.0 6 51 5 96 65 0.0 6 61 4 97 72 0.0 6 72 98 57 0.0 6 52 5 99 61 0.0 6 53 8 100 74 0.0 6 69 5 101 75 0.0 6 68 4 1 1 1 102 63 0.0 6 62 1 103 93 0.0 6 89 4 104 83 0.0 6 81 2 105 164 0.0 6 158 6 106 89 0.0 6 86 2 1 107 67 0.0 6 66 1 108 94 0.0 6 90 3 0 1 109 77 0.0 6 74 2 0 1 110 92 0.0 6 82 10 111 101 0.0 6 98 3 112 93 0.0 6 91 2 113 101 0.0 6 95 6 114 112 0.0 6 107 5 115 94 0.0 6 90 3 0 0 0 0 1 116 105 0.0 6 97 4 1 1 2 117 100 0.0 6 98 1 1 118 125 0.0 6 123 1 0 1 119 153 0.0 6 150 3 120 143 0.0 6 142 0 1 121 126 0.0 6 124 2 122 149 0.0 6 145 4 123 134 0.0 6 130 3 1 124 153 0.0 6 149 4 125 155 0.0 6 149 6 126 186 0.0 6 179 7 127 185 0.0 6 180 5 128 185 0.0 6 180 3 0 0 1 0 1 129 203 0.0 6 201 2 130 261 0.0 6 253 4 1 3 131 297 0.0 6 283 9 2 1 0 1 1 132 316 0.0 6 313 2 0 1 133 298 0.0 6 293 4 0 1 134 344 0.0 6 338 5 0 0 1 135 438 0.0 6 428 7 0 1 0 2 136 322 0.0 6 318 3 0 0 1 137 341 0.0 6 337 4 138 338 0.0 6 332 4 1 1 139 390 0.0 6 382 8 140 509 0.0 6 502 6 0 0 1 141 607 0.0 6 594 11 0 1 1 142 572 0.0 6 561 10 0 0 1 143 627 0.0 6 614 8 2 1 2 144 733 0.0 6 723 9 0 0 1 145 630 0.0 6 624 5 0 1 146 576 0.0 6 572 2 1 0 1 147 647 0.0 6 639 8 148 804 0.0 6 790 13 1 149 897 0.0 6 881 13 1 0 0 2 150 932 0.0 6 912 17 0 2 0 1 151 1065 0.0 6 1043 21 0 1 152 988 0.0 6 968 19 0 1 153 981 0.0 6 958 18 2 0 3 154 983 0.0 6 958 20 2 2 1 155 773 0.0 6 761 10 1 0 0 1 156 854 0.0 6 843 9 2 157 955 0.0 6 946 7 2 158 986 0.0 6 973 13 159 818 0.0 6 809 7 1 0 0 1 160 851 0.0 6 833 16 1 0 1 161 783 0.0 6 768 13 0 0 1 0 1 162 728 0.0 6 720 7 0 0 1 163 710 0.0 6 695 14 1 164 687 0.0 6 676 10 0 0 0 1 165 657 0.0 6 647 10 166 712 0.0 6 698 14 167 776 0.0 6 769 6 1 168 879 0.0 6 864 13 1 0 0 0 1 169 730 0.0 6 725 4 0 1 170 657 0.0 6 638 18 0 1 171 848 0.0 6 838 8 1 0 1 172 662 0.0 6 652 10 173 751 0.0 6 742 8 1 174 750 0.0 6 731 18 0 1 175 990 0.0 6 968 22 176 728 0.0 6 714 11 1 0 2 177 813 0.0 6 801 10 0 1 1 178 625 0.0 6 607 17 1 179 765 0.0 6 750 14 1 180 644 0.0 6 628 14 1 0 0 1 181 743 0.0 6 723 18 2 182 486 0.0 6 478 8 183 707 0.0 6 695 10 1 1 184 574 0.0 6 567 5 0 0 1 1 185 861 0.0 6 850 11 186 1203 0.0 6 1172 29 0 1 0 0 1 187 797 0.0 6 774 22 1 188 768 0.0 6 749 18 0 0 1 189 798 0.0 6 783 15 190 1135 0.0 6 1096 36 1 2 191 1015 0.0 6 996 18 1 192 843 0.0 6 818 23 2 193 495 0.0 6 480 13 1 0 1 194 665 0.0 6 644 19 2 195 747 0.0 6 730 16 1 196 1395 0.0 6 1343 47 1 0 2 1 1 197 674 0.0 6 660 12 0 0 2 198 880 0.0 6 844 34 1 0 1 199 1178 0.0 6 1152 26 200 476 0.0 6 458 17 0 1 201 615 0.0 6 594 19 0 1 1 202 345 0.0 6 341 4 203 244 0.0 6 234 10 204 320 0.0 6 317 3 205 164 0.0 6 158 6 206 147 0.0 6 144 3 207 138 0.0 6 137 1 208 202 0.0 6 195 7 209 145 0.0 6 141 3 1 210 167 0.0 6 161 6 211 154 0.0 6 151 3 212 221 0.0 6 214 6 0 1 213 99 0.0 6 92 6 1 214 139 0.0 6 135 4 215 185 0.0 6 182 3 216 213 0.0 6 206 7 217 175 0.0 6 170 4 0 0 1 218 160 0.0 6 153 6 0 1 219 188 0.0 6 181 7 220 66 0.0 6 61 5 221 92 0.0 6 88 4 222 148 0.0 6 143 5 223 218 0.0 6 212 6 224 73 0.0 6 72 1 225 155 0.0 6 151 4 226 95 0.0 6 85 9 1 227 73 0.0 6 70 3 228 80 0.0 6 77 3 229 76 0.0 6 74 2 230 49 0.0 6 47 2 231 37 0.0 6 32 4 1 232 126 0.0 6 121 5 233 130 0.0 6 125 5 234 16 0.0 6 15 1 235 21 0.0 6 20 1 236 11 0.0 6 11 237 12 0.0 6 12 238 4 0.0 6 4 239 12 0.0 6 12 240 33 0.0 6 32 1 241 27 0.0 6 25 2 242 45 0.0 6 42 2 1 243 39 0.0 6 37 2 244 26 0.0 6 24 1 0 0 1 245 15 0.0 6 14 0 0 1 246 9 0.0 6 8 1 247 6 0.0 6 6 248 10 0.0 6 10 249 2 0.0 6 2 250 2 0.0 6 2 251 4 0.0 6 4 252 2 0.0 6 2 253 5 0.0 6 4 1 254 5 0.0 6 5 255 1 0.0 6 1 278 4 0.0 6 4 279 11 0.0 6 10 1 280 45 0.0 6 43 1 0 1 281 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1065 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 6 72 15.7 1 0 0 72 71 7 0.0 6 7 72 2 0.0 6 2 73 12 0.0 6 12 74 6 0.0 6 6 75 12 0.0 6 12 76 12 0.0 6 12 77 4 0.0 6 4 78 6 0.0 6 6 79 10 0.0 6 10 80 9 0.0 6 9 81 11 0.0 6 11 82 6 0.0 6 6 83 10 0.0 6 10 84 4 0.0 6 4 85 6 0.0 6 6 86 5 0.0 6 5 87 6 0.0 6 6 88 1 0.0 6 1 89 3 0.0 6 3 90 4 0.0 6 4 91 10 0.0 6 10 92 11 0.0 6 11 93 13 0.0 6 13 94 14 0.0 6 14 95 4 0.0 6 4 96 7 0.0 6 7 97 3 0.0 6 3 98 6 0.0 6 6 99 11 0.0 6 11 100 12 0.0 6 12 101 18 0.0 6 18 102 4 0.0 6 4 103 4 0.0 6 4 104 6 0.0 6 6 105 22 0.0 6 22 106 10 0.0 6 10 107 15 0.0 6 15 108 4 0.0 6 4 109 13 0.0 6 13 110 3 0.0 6 3 111 5 0.0 6 4 0 0 0 1 112 3 0.0 6 3 113 5 0.0 6 5 114 16 0.0 6 15 1 115 11 0.0 6 11 116 8 0.0 6 8 117 11 0.0 6 11 118 13 0.0 6 12 0 0 0 1 119 5 0.0 6 5 120 26 0.0 6 24 0 0 2 121 6 0.0 6 6 122 21 0.0 6 21 123 5 0.0 6 5 124 4 0.0 6 4 125 8 0.0 6 8 126 17 0.0 6 17 127 13 0.0 6 13 128 2 0.0 6 2 129 1 0.0 6 1 130 2 0.0 6 2 132 6 0.0 6 6 133 11 0.0 6 11 134 20 0.0 6 20 135 8 0.0 6 8 136 9 0.0 6 9 137 7 0.0 6 7 138 9 0.0 6 9 139 14 0.0 6 14 140 10 0.0 6 10 141 6 0.0 6 6 142 7 0.0 6 7 143 11 0.0 6 11 144 12 0.0 6 12 145 3 0.0 6 3 146 10 0.0 6 10 147 5 0.0 6 5 148 17 0.0 6 17 149 5 0.0 6 5 150 9 0.0 6 9 151 6 0.0 6 6 152 4 0.0 6 4 153 2 0.0 6 2 154 8 0.0 6 8 155 3 0.0 6 3 156 8 0.0 6 8 157 28 0.0 6 28 158 11 0.0 6 11 160 3 0.0 6 3 161 3 0.0 6 3 162 3 0.0 6 3 163 1 0.0 6 1 164 3 0.0 6 3 165 15 0.0 6 15 166 10 0.0 6 10 167 5 0.0 6 5 168 4 0.0 6 4 169 8 0.0 6 8 170 1 0.0 6 1 171 3 0.0 6 3 172 8 0.0 6 8 173 7 0.0 6 7 174 3 0.0 6 3 175 8 0.0 6 8 176 7 0.0 6 6 1 177 15 0.0 6 15 178 16 0.0 6 16 179 5 0.0 6 5 180 4 0.0 6 4 181 6 0.0 6 6 183 5 0.0 6 5 184 9 0.0 6 9 185 5 0.0 6 5 186 12 0.0 6 12 187 3 0.0 6 3 188 17 0.0 6 17 189 3 0.0 6 3 190 6 0.0 6 6 191 2 0.0 6 2 192 1 0.0 6 1 193 4 0.0 6 4 194 3 0.0 6 3 199 2 0.0 6 2 200 2 0.0 6 2 202 1 0.0 6 1 203 2 0.0 6 2 204 1 0.0 6 1 205 1 0.0 6 0 1 207 3 0.0 6 3 208 1 0.0 6 1 210 1 0.0 6 1 211 3 0.0 6 3 212 1 0.0 6 1 215 2 0.0 6 2 218 1 0.0 6 0 1 221 1 0.0 6 1 222 4 0.0 6 4 227 1 0.0 6 1 251 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_09_208_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_09_435_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_09_208_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_09_435_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.45 s (62 µs/read; 0.96 M reads/minute). === Summary === Total read pairs processed: 39,192 Read 1 with adapter: 39,189 (100.0%) Read 2 with adapter: 39,185 (100.0%) == Read fate breakdown == Pairs that were too short: 20 (0.1%) Pairs discarded as untrimmed: 10 (0.0%) Pairs written (passing filters): 39,162 (99.9%) Total basepairs processed: 15,715,778 bp Read 1: 8,483,038 bp Read 2: 7,232,740 bp Total written (filtered): 2,470,980 bp (15.7%) Read 1: 1,279,157 bp Read 2: 1,191,823 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 37966 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 612.4 0 0 1 4 2 153.1 1 0 2 6 1 9.6 1 0 0 1 7 2 2.4 2 1 0 1 9 3 0.1 2 3 14 1 0.0 4 1 15 2 0.0 4 1 1 16 2 0.0 4 1 0 0 1 19 3 0.0 5 2 1 20 32 0.0 6 28 4 21 22 0.0 6 21 1 22 23 0.0 6 23 23 20 0.0 6 20 24 18 0.0 6 17 0 1 25 9 0.0 6 9 26 16 0.0 6 16 27 11 0.0 6 11 28 8 0.0 6 7 1 29 6 0.0 6 6 30 2 0.0 6 2 31 5 0.0 6 5 32 3 0.0 6 3 33 4 0.0 6 4 34 8 0.0 6 8 35 1 0.0 6 1 36 7 0.0 6 7 37 9 0.0 6 9 38 3 0.0 6 3 39 9 0.0 6 8 1 40 8 0.0 6 8 41 3 0.0 6 3 42 5 0.0 6 5 43 8 0.0 6 8 44 4 0.0 6 4 45 12 0.0 6 11 0 0 1 46 2 0.0 6 2 47 4 0.0 6 3 1 48 12 0.0 6 11 1 49 8 0.0 6 7 1 50 5 0.0 6 4 1 51 7 0.0 6 7 52 8 0.0 6 8 53 8 0.0 6 8 54 13 0.0 6 12 1 55 9 0.0 6 9 56 3 0.0 6 2 0 1 57 41 0.0 6 40 0 0 1 58 16 0.0 6 16 60 11 0.0 6 10 0 0 1 61 10 0.0 6 10 62 2 0.0 6 2 63 16 0.0 6 16 64 9 0.0 6 9 65 1 0.0 6 1 66 9 0.0 6 9 67 6 0.0 6 6 68 4 0.0 6 4 69 14 0.0 6 13 0 0 1 70 9 0.0 6 8 0 0 1 71 1 0.0 6 1 72 16 0.0 6 16 73 10 0.0 6 10 74 10 0.0 6 10 75 7 0.0 6 7 76 12 0.0 6 11 0 0 1 77 6 0.0 6 6 78 15 0.0 6 14 1 79 30 0.0 6 30 80 9 0.0 6 8 1 81 20 0.0 6 19 0 0 0 1 82 12 0.0 6 12 83 9 0.0 6 9 84 28 0.0 6 28 85 17 0.0 6 17 86 5 0.0 6 5 87 15 0.0 6 15 88 11 0.0 6 10 1 89 5 0.0 6 5 90 8 0.0 6 8 91 20 0.0 6 20 92 10 0.0 6 9 1 93 24 0.0 6 22 2 94 22 0.0 6 22 95 6 0.0 6 6 96 22 0.0 6 22 97 23 0.0 6 23 99 23 0.0 6 22 1 100 16 0.0 6 16 101 11 0.0 6 11 102 17 0.0 6 17 103 17 0.0 6 17 104 15 0.0 6 15 105 17 0.0 6 16 1 106 28 0.0 6 27 1 107 20 0.0 6 20 108 29 0.0 6 27 1 1 109 41 0.0 6 41 110 15 0.0 6 15 111 28 0.0 6 28 112 25 0.0 6 24 1 113 24 0.0 6 24 114 37 0.0 6 36 1 115 31 0.0 6 31 116 20 0.0 6 19 1 117 38 0.0 6 36 1 0 0 0 1 118 27 0.0 6 26 1 119 13 0.0 6 12 1 120 25 0.0 6 23 1 1 121 44 0.0 6 42 1 0 1 122 28 0.0 6 27 0 0 0 0 1 123 54 0.0 6 54 124 56 0.0 6 53 2 0 1 125 19 0.0 6 19 126 42 0.0 6 40 2 127 72 0.0 6 70 1 1 128 32 0.0 6 32 129 79 0.0 6 78 1 130 64 0.0 6 63 0 0 1 131 42 0.0 6 42 132 68 0.0 6 68 133 43 0.0 6 43 134 48 0.0 6 47 1 135 48 0.0 6 47 0 0 0 0 1 136 36 0.0 6 36 137 49 0.0 6 47 2 138 53 0.0 6 53 139 77 0.0 6 76 0 1 140 350 0.0 6 348 2 141 566 0.0 6 566 142 861 0.0 6 861 143 478 0.0 6 478 144 477 0.0 6 474 3 145 1077 0.0 6 1075 1 0 1 146 346 0.0 6 345 1 147 1818 0.0 6 1814 3 0 1 148 942 0.0 6 941 1 149 423 0.0 6 423 150 1856 0.0 6 1855 1 151 325 0.0 6 324 1 152 182 0.0 6 181 1 153 116 0.0 6 116 154 77 0.0 6 76 1 155 207 0.0 6 206 1 156 1028 0.0 6 1026 2 157 253 0.0 6 252 1 158 331 0.0 6 330 1 159 135 0.0 6 135 160 85 0.0 6 85 161 243 0.0 6 241 2 162 612 0.0 6 611 1 163 522 0.0 6 521 1 164 128 0.0 6 128 165 354 0.0 6 354 166 726 0.0 6 725 1 167 102 0.0 6 102 168 113 0.0 6 112 1 169 163 0.0 6 163 170 710 0.0 6 708 2 171 136 0.0 6 136 172 440 0.0 6 438 2 173 367 0.0 6 367 174 64 0.0 6 64 175 70 0.0 6 70 176 93 0.0 6 93 177 82 0.0 6 82 178 257 0.0 6 257 179 67 0.0 6 67 180 224 0.0 6 223 0 0 0 1 181 79 0.0 6 79 182 43 0.0 6 41 2 183 73 0.0 6 72 1 184 101 0.0 6 99 2 185 261 0.0 6 260 1 186 172 0.0 6 171 0 0 0 1 187 89 0.0 6 89 188 116 0.0 6 116 189 117 0.0 6 117 190 67 0.0 6 66 1 191 103 0.0 6 103 192 83 0.0 6 83 193 116 0.0 6 115 1 194 175 0.0 6 175 195 1060 0.0 6 1060 196 319 0.0 6 319 197 147 0.0 6 147 198 168 0.0 6 168 199 216 0.0 6 215 1 200 315 0.0 6 314 1 201 895 0.0 6 893 1 0 0 0 1 202 152 0.0 6 152 203 127 0.0 6 127 204 95 0.0 6 94 1 205 116 0.0 6 115 1 206 111 0.0 6 111 207 303 0.0 6 300 2 0 0 1 208 158 0.0 6 158 209 170 0.0 6 169 1 210 223 0.0 6 223 211 101 0.0 6 100 1 212 132 0.0 6 131 1 213 195 0.0 6 191 3 0 0 0 1 214 209 0.0 6 207 2 215 317 0.0 6 317 216 166 0.0 6 164 2 217 117 0.0 6 116 1 218 150 0.0 6 149 1 219 167 0.0 6 167 220 127 0.0 6 125 2 221 79 0.0 6 78 0 0 0 0 0 1 222 71 0.0 6 71 223 100 0.0 6 99 1 224 128 0.0 6 127 1 225 161 0.0 6 161 226 229 0.0 6 227 2 227 196 0.0 6 196 228 148 0.0 6 147 1 229 152 0.0 6 150 2 230 210 0.0 6 209 1 231 204 0.0 6 203 1 232 287 0.0 6 287 233 284 0.0 6 284 234 319 0.0 6 319 235 392 0.0 6 391 1 236 408 0.0 6 408 237 247 0.0 6 246 1 238 232 0.0 6 229 3 239 265 0.0 6 265 240 513 0.0 6 512 1 241 811 0.0 6 810 1 242 1273 0.0 6 1273 243 106 0.0 6 106 244 102 0.0 6 102 245 85 0.0 6 84 1 246 123 0.0 6 123 247 186 0.0 6 186 248 319 0.0 6 318 1 249 54 0.0 6 54 250 57 0.0 6 57 251 71 0.0 6 71 252 83 0.0 6 82 1 253 70 0.0 6 70 254 89 0.0 6 88 1 255 53 0.0 6 53 256 49 0.0 6 49 257 91 0.0 6 91 258 41 0.0 6 41 259 77 0.0 6 77 260 45 0.0 6 45 261 47 0.0 6 47 262 74 0.0 6 73 1 263 88 0.0 6 87 1 264 88 0.0 6 88 265 76 0.0 6 76 266 63 0.0 6 63 267 88 0.0 6 88 268 70 0.0 6 70 269 83 0.0 6 83 270 115 0.0 6 114 1 271 69 0.0 6 67 2 272 64 0.0 6 64 273 80 0.0 6 80 274 119 0.0 6 119 275 173 0.0 6 172 1 276 107 0.0 6 107 277 69 0.0 6 69 278 52 0.0 6 52 279 154 0.0 6 152 2 280 276 0.0 6 275 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1223 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 2 0.0 6 2 66 1 0.0 6 1 69 1 0.0 6 1 70 2 0.0 6 2 71 11 0.0 6 11 72 35 0.0 6 35 73 37 0.0 6 37 74 16 0.0 6 16 75 23 0.0 6 23 76 35 0.0 6 34 1 77 22 0.0 6 22 78 72 0.0 6 72 79 20 0.0 6 20 80 27 0.0 6 26 1 81 81 0.0 6 81 82 6 0.0 6 5 1 83 5 0.0 6 4 1 84 1 0.0 6 1 85 2 0.0 6 2 86 11 0.0 6 11 87 40 0.0 6 40 88 4 0.0 6 4 89 8 0.0 6 8 90 1 0.0 6 1 91 1 0.0 6 1 92 8 0.0 6 8 93 21 0.0 6 21 94 13 0.0 6 13 95 6 0.0 6 6 96 15 0.0 6 15 97 27 0.0 6 27 98 1 0.0 6 1 99 5 0.0 6 5 100 8 0.0 6 8 101 19 0.0 6 18 1 102 4 0.0 6 4 103 12 0.0 6 12 104 14 0.0 6 14 106 1 0.0 6 1 107 1 0.0 6 1 108 3 0.0 6 3 109 8 0.0 6 8 110 4 0.0 6 4 111 9 0.0 6 9 112 1 0.0 6 1 115 2 0.0 6 2 116 12 0.0 6 12 117 5 0.0 6 4 1 118 3 0.0 6 3 120 1 0.0 6 1 121 1 0.0 6 1 124 1 0.0 6 1 125 15 0.0 6 15 126 49 0.0 6 49 127 1 0.0 6 1 129 1 0.0 6 1 130 1 0.0 6 1 131 11 0.0 6 11 132 39 0.0 6 39 133 1 0.0 6 1 137 1 0.0 6 1 138 3 0.0 6 3 140 2 0.0 6 2 141 9 0.0 6 9 144 1 0.0 6 1 149 1 0.0 6 1 151 1 0.0 6 1 153 1 0.0 6 1 154 1 0.0 6 1 160 1 0.0 6 1 161 1 0.0 6 1 166 5 0.0 6 5 167 9 0.0 6 9 168 1 0.0 6 1 169 2 0.0 6 2 171 1 0.0 6 1 172 5 0.0 6 5 173 5 0.0 6 5 174 4 0.0 6 4 175 1 0.0 6 1 176 1 0.0 6 1 177 2 0.0 6 2 178 8 0.0 6 8 179 28 0.0 6 28 180 3 0.0 6 3 183 3 0.0 6 3 184 1 0.0 6 1 185 6 0.0 6 6 187 1 0.0 6 1 189 5 0.0 6 5 190 4 0.0 6 4 191 2 0.0 6 2 193 1 0.0 6 1 194 1 0.0 6 1 195 2 0.0 6 2 196 6 0.0 6 5 1 198 1 0.0 6 1 200 2 0.0 6 2 201 13 0.0 6 13 202 5 0.0 6 5 203 4 0.0 6 4 204 10 0.0 6 9 1 205 41 0.0 6 41 206 142 0.0 6 142 207 9 0.0 6 8 0 0 0 0 0 1 209 1 0.0 6 1 214 1 0.0 6 1 216 1 0.0 6 1 217 5 0.0 6 5 218 2 0.0 6 2 219 1 0.0 6 1 225 1 0.0 6 1 226 2 0.0 6 2 228 1 0.0 6 1 229 2 0.0 6 2 232 3 0.0 6 3 235 1 0.0 6 1 238 4 0.0 6 4 239 6 0.0 6 6 240 15 0.0 6 15 242 1 0.0 6 1 245 1 0.0 6 1 247 1 0.0 6 1 251 1 0.0 6 1 262 5 0.0 6 5 263 20 0.0 6 20 265 1 0.0 6 1 266 1 0.0 6 1 272 1 0.0 6 1 281 1 0.0 6 1 285 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 37941 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 2 2.4 2 1 1 11 1 0.0 3 1 12 1 0.0 3 1 13 2 0.0 3 1 1 14 4 0.0 4 3 0 1 15 1 0.0 4 1 16 2 0.0 4 1 0 0 1 17 11 0.0 5 11 18 2 0.0 5 2 19 13 0.0 5 11 2 20 27 0.0 6 27 21 16 0.0 6 16 22 15 0.0 6 13 2 23 8 0.0 6 7 1 24 11 0.0 6 11 25 5 0.0 6 4 1 26 3 0.0 6 3 27 10 0.0 6 10 28 8 0.0 6 8 29 10 0.0 6 10 30 7 0.0 6 6 1 31 6 0.0 6 6 32 9 0.0 6 8 0 0 1 33 2 0.0 6 1 1 34 4 0.0 6 4 35 5 0.0 6 5 36 4 0.0 6 3 1 37 3 0.0 6 3 38 4 0.0 6 4 39 10 0.0 6 10 40 3 0.0 6 3 41 4 0.0 6 4 42 9 0.0 6 9 43 14 0.0 6 11 2 0 1 44 10 0.0 6 8 2 45 21 0.0 6 20 1 46 12 0.0 6 12 47 12 0.0 6 11 1 48 14 0.0 6 13 1 49 9 0.0 6 8 1 50 7 0.0 6 7 51 20 0.0 6 20 52 14 0.0 6 14 53 9 0.0 6 7 2 54 20 0.0 6 20 55 15 0.0 6 14 1 56 15 0.0 6 13 2 57 20 0.0 6 19 0 1 58 11 0.0 6 11 59 24 0.0 6 23 0 0 0 0 0 1 60 17 0.0 6 15 2 61 29 0.0 6 25 3 0 0 0 1 62 11 0.0 6 10 1 63 20 0.0 6 20 64 26 0.0 6 21 5 65 23 0.0 6 23 66 31 0.0 6 30 1 67 17 0.0 6 15 2 68 26 0.0 6 22 3 1 69 31 0.0 6 30 0 0 0 0 0 1 70 80 0.0 6 77 3 71 40 0.0 6 39 1 72 46 0.0 6 45 1 73 40 0.0 6 40 74 24 0.0 6 24 75 27 0.0 6 26 1 76 16 0.0 6 13 2 0 1 77 36 0.0 6 35 1 78 19 0.0 6 18 0 0 0 1 79 17 0.0 6 15 2 80 37 0.0 6 37 81 32 0.0 6 30 2 82 39 0.0 6 38 1 83 66 0.0 6 62 3 0 1 84 49 0.0 6 48 1 85 51 0.0 6 47 3 0 0 1 86 75 0.0 6 75 87 49 0.0 6 46 3 88 60 0.0 6 58 2 89 89 0.0 6 87 2 90 43 0.0 6 39 3 1 91 76 0.0 6 73 3 92 71 0.0 6 69 1 0 0 1 93 72 0.0 6 72 94 114 0.0 6 107 6 0 1 95 53 0.0 6 52 1 96 36 0.0 6 35 1 97 42 0.0 6 41 0 0 0 1 98 40 0.0 6 40 99 39 0.0 6 38 1 100 40 0.0 6 40 101 51 0.0 6 50 1 102 42 0.0 6 39 3 103 52 0.0 6 50 2 104 73 0.0 6 70 3 105 33 0.0 6 33 106 69 0.0 6 67 2 107 104 0.0 6 102 2 108 51 0.0 6 50 1 109 55 0.0 6 53 2 110 69 0.0 6 66 3 111 82 0.0 6 79 3 112 77 0.0 6 74 2 0 0 0 0 1 113 103 0.0 6 98 5 114 74 0.0 6 74 115 95 0.0 6 90 4 0 0 0 0 1 116 76 0.0 6 72 4 117 78 0.0 6 77 1 118 91 0.0 6 89 2 119 85 0.0 6 83 2 120 105 0.0 6 103 1 1 121 97 0.0 6 94 3 122 104 0.0 6 102 2 123 106 0.0 6 104 2 124 98 0.0 6 97 1 125 110 0.0 6 110 126 109 0.0 6 107 2 127 137 0.0 6 136 1 128 132 0.0 6 131 1 129 156 0.0 6 152 4 130 132 0.0 6 130 2 131 152 0.0 6 150 2 132 169 0.0 6 169 133 180 0.0 6 178 2 134 176 0.0 6 173 2 1 135 170 0.0 6 167 3 136 237 0.0 6 235 2 137 299 0.0 6 297 2 138 389 0.0 6 384 5 139 488 0.0 6 487 0 0 1 140 615 0.0 6 612 3 141 776 0.0 6 774 2 142 581 0.0 6 580 1 143 626 0.0 6 622 4 144 1122 0.0 6 1118 3 0 0 0 0 1 145 768 0.0 6 765 3 146 1543 0.0 6 1535 8 147 974 0.0 6 969 5 148 551 0.0 6 547 3 0 0 1 149 1524 0.0 6 1519 4 0 1 150 446 0.0 6 442 4 151 405 0.0 6 397 7 1 152 404 0.0 6 396 6 0 1 1 153 523 0.0 6 519 1 0 1 1 1 154 573 0.0 6 564 8 0 0 1 155 1041 0.0 6 1033 7 0 0 0 0 1 156 443 0.0 6 436 7 157 471 0.0 6 466 4 1 158 447 0.0 6 442 5 159 417 0.0 6 409 7 0 1 160 588 0.0 6 578 10 161 630 0.0 6 627 3 162 544 0.0 6 538 5 0 0 0 1 163 384 0.0 6 380 4 164 623 0.0 6 616 7 165 528 0.0 6 523 3 0 2 166 306 0.0 6 298 8 167 355 0.0 6 350 3 0 1 0 0 1 168 518 0.0 6 507 11 169 617 0.0 6 606 11 170 608 0.0 6 590 18 171 624 0.0 6 611 12 0 0 0 0 1 172 354 0.0 6 343 9 2 173 340 0.0 6 332 8 174 473 0.0 6 466 7 175 414 0.0 6 413 1 176 313 0.0 6 311 2 177 374 0.0 6 370 4 178 338 0.0 6 334 3 1 179 285 0.0 6 285 180 210 0.0 6 209 0 1 181 264 0.0 6 261 3 182 249 0.0 6 248 1 183 250 0.0 6 247 2 0 0 1 184 166 0.0 6 164 2 185 139 0.0 6 138 1 186 151 0.0 6 151 187 303 0.0 6 301 2 188 291 0.0 6 291 189 310 0.0 6 309 1 190 238 0.0 6 237 1 191 231 0.0 6 231 192 340 0.0 6 337 3 193 279 0.0 6 277 2 194 338 0.0 6 336 2 195 269 0.0 6 267 2 196 208 0.0 6 206 2 197 172 0.0 6 172 198 657 0.0 6 655 2 199 195 0.0 6 194 1 200 152 0.0 6 149 3 201 78 0.0 6 78 202 94 0.0 6 94 203 146 0.0 6 145 1 204 93 0.0 6 92 1 205 90 0.0 6 90 206 95 0.0 6 95 207 102 0.0 6 102 208 66 0.0 6 66 209 97 0.0 6 97 210 38 0.0 6 38 211 23 0.0 6 23 212 44 0.0 6 44 213 69 0.0 6 69 214 107 0.0 6 104 3 215 88 0.0 6 88 216 59 0.0 6 58 1 217 68 0.0 6 68 218 78 0.0 6 76 2 219 60 0.0 6 60 220 105 0.0 6 104 1 221 50 0.0 6 49 1 222 10 0.0 6 10 223 20 0.0 6 20 224 26 0.0 6 26 225 37 0.0 6 37 226 14 0.0 6 13 1 227 59 0.0 6 58 1 228 9 0.0 6 9 229 6 0.0 6 6 230 17 0.0 6 17 231 12 0.0 6 12 232 14 0.0 6 14 233 4 0.0 6 4 234 1 0.0 6 1 235 1 0.0 6 1 236 2 0.0 6 2 237 2 0.0 6 2 238 5 0.0 6 5 239 3 0.0 6 3 240 2 0.0 6 2 241 2 0.0 6 2 242 2 0.0 6 2 243 2 0.0 6 2 244 3 0.0 6 3 245 1 0.0 6 1 246 1 0.0 6 1 247 2 0.0 6 2 248 4 0.0 6 4 250 1 0.0 6 1 251 1 0.0 6 1 255 6 0.0 6 6 256 1 0.0 6 1 257 1 0.0 6 1 281 2 0.0 6 2 282 15 0.0 6 15 283 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1244 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 46 1 0.0 6 0 1 68 7 0.0 6 7 69 18 0.0 6 18 70 32 0.0 6 32 71 47 0.0 6 47 72 13 0.0 6 13 73 25 0.0 6 25 74 46 0.0 6 46 75 24 0.0 6 24 76 91 0.0 6 90 0 0 1 77 43 0.0 6 42 0 0 0 1 78 15 0.0 6 15 79 88 0.0 6 88 80 6 0.0 6 4 0 0 0 2 81 2 0.0 6 2 82 1 0.0 6 1 83 1 0.0 6 1 84 23 0.0 6 23 85 46 0.0 6 45 0 0 0 1 86 6 0.0 6 6 87 10 0.0 6 9 0 0 0 1 90 12 0.0 6 12 91 28 0.0 6 28 92 19 0.0 6 19 93 3 0.0 6 3 94 17 0.0 6 16 0 0 1 95 36 0.0 6 36 96 2 0.0 6 2 97 3 0.0 6 3 98 5 0.0 6 5 99 36 0.0 6 36 100 7 0.0 6 7 101 22 0.0 6 22 102 16 0.0 6 15 0 0 1 104 2 0.0 6 2 105 6 0.0 6 5 0 0 0 1 106 4 0.0 6 4 107 7 0.0 6 7 108 2 0.0 6 2 109 11 0.0 6 11 111 1 0.0 6 1 112 2 0.0 6 2 113 3 0.0 6 3 114 13 0.0 6 13 115 5 0.0 6 5 116 3 0.0 6 3 117 1 0.0 6 1 118 3 0.0 6 3 119 1 0.0 6 1 120 2 0.0 6 2 122 1 0.0 6 1 123 7 0.0 6 7 124 53 0.0 6 53 125 9 0.0 6 9 126 1 0.0 6 1 128 2 0.0 6 2 129 7 0.0 6 7 130 62 0.0 6 62 131 2 0.0 6 2 133 1 0.0 6 1 134 2 0.0 6 1 0 0 0 1 136 5 0.0 6 5 137 1 0.0 6 1 138 2 0.0 6 2 139 8 0.0 6 8 148 1 0.0 6 1 149 1 0.0 6 1 151 1 0.0 6 1 156 1 0.0 6 1 157 2 0.0 6 2 161 1 0.0 6 1 163 2 0.0 6 2 164 3 0.0 6 3 165 9 0.0 6 9 166 1 0.0 6 1 167 1 0.0 6 1 170 6 0.0 6 6 171 4 0.0 6 4 175 5 0.0 6 5 176 6 0.0 6 6 177 42 0.0 6 42 182 3 0.0 6 3 183 2 0.0 6 2 187 3 0.0 6 3 188 10 0.0 6 10 189 3 0.0 6 3 191 1 0.0 6 1 194 2 0.0 6 2 198 1 0.0 6 1 199 8 0.0 6 8 200 5 0.0 6 5 201 1 0.0 6 1 202 10 0.0 6 9 0 0 0 1 203 28 0.0 6 28 204 91 0.0 6 91 205 3 0.0 6 3 208 1 0.0 6 0 0 0 0 1 211 1 0.0 6 1 215 2 0.0 6 2 238 2 0.0 6 2 261 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_10_209_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_10_436_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_10_209_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_10_436_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 1.55 s (80 µs/read; 0.75 M reads/minute). === Summary === Total read pairs processed: 19,362 Read 1 with adapter: 19,360 (100.0%) Read 2 with adapter: 19,353 (100.0%) == Read fate breakdown == Pairs that were too short: 15 (0.1%) Pairs discarded as untrimmed: 10 (0.1%) Pairs written (passing filters): 19,337 (99.9%) Total basepairs processed: 7,295,289 bp Read 1: 3,920,416 bp Read 2: 3,374,873 bp Total written (filtered): 1,324,816 bp (18.2%) Read 1: 662,106 bp Read 2: 662,710 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 18360 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 75.6 1 0 1 8 1 0.3 2 1 9 1 0.1 2 0 1 15 1 0.0 4 0 1 19 1 0.0 5 1 20 40 0.0 6 40 21 8 0.0 6 8 22 12 0.0 6 11 1 23 11 0.0 6 11 24 9 0.0 6 8 1 25 8 0.0 6 8 26 2 0.0 6 2 27 4 0.0 6 4 28 3 0.0 6 3 29 3 0.0 6 2 1 30 1 0.0 6 1 31 1 0.0 6 1 33 2 0.0 6 2 38 4 0.0 6 4 39 3 0.0 6 3 42 2 0.0 6 2 43 1 0.0 6 1 47 3 0.0 6 3 48 2 0.0 6 2 50 1 0.0 6 1 51 6 0.0 6 6 52 5 0.0 6 5 53 2 0.0 6 2 54 6 0.0 6 6 55 4 0.0 6 3 0 1 56 3 0.0 6 3 57 3 0.0 6 3 60 2 0.0 6 2 61 2 0.0 6 2 62 1 0.0 6 1 63 2 0.0 6 2 64 1 0.0 6 1 65 1 0.0 6 1 66 1 0.0 6 1 67 5 0.0 6 5 69 4 0.0 6 4 70 4 0.0 6 4 71 3 0.0 6 3 72 6 0.0 6 6 73 6 0.0 6 6 74 5 0.0 6 4 1 75 5 0.0 6 5 76 12 0.0 6 12 77 5 0.0 6 5 78 5 0.0 6 5 79 7 0.0 6 7 80 2 0.0 6 1 1 81 9 0.0 6 9 82 6 0.0 6 6 83 4 0.0 6 3 1 84 5 0.0 6 5 85 3 0.0 6 3 86 1 0.0 6 1 87 3 0.0 6 3 88 2 0.0 6 2 89 4 0.0 6 4 90 1 0.0 6 1 91 2 0.0 6 2 92 2 0.0 6 2 93 1 0.0 6 1 94 1 0.0 6 1 95 2 0.0 6 2 96 2 0.0 6 2 97 5 0.0 6 5 98 2 0.0 6 2 99 2 0.0 6 2 100 3 0.0 6 3 101 3 0.0 6 3 102 5 0.0 6 5 103 6 0.0 6 6 104 9 0.0 6 9 105 8 0.0 6 6 1 1 106 17 0.0 6 17 107 6 0.0 6 6 108 5 0.0 6 5 109 9 0.0 6 9 110 6 0.0 6 6 111 18 0.0 6 18 112 22 0.0 6 22 113 19 0.0 6 19 114 13 0.0 6 13 115 17 0.0 6 17 116 20 0.0 6 20 117 22 0.0 6 22 118 9 0.0 6 8 1 119 26 0.0 6 25 1 120 32 0.0 6 32 121 10 0.0 6 10 122 4 0.0 6 4 123 6 0.0 6 6 124 3 0.0 6 3 125 7 0.0 6 7 126 12 0.0 6 12 127 12 0.0 6 12 128 12 0.0 6 12 129 15 0.0 6 15 130 18 0.0 6 18 131 14 0.0 6 14 132 27 0.0 6 27 133 16 0.0 6 16 134 21 0.0 6 21 135 25 0.0 6 25 136 22 0.0 6 22 137 31 0.0 6 31 138 27 0.0 6 27 139 33 0.0 6 33 140 35 0.0 6 35 141 55 0.0 6 55 142 58 0.0 6 58 143 275 0.0 6 274 1 144 213 0.0 6 210 3 145 700 0.0 6 700 146 955 0.0 6 952 3 147 340 0.0 6 339 1 148 358 0.0 6 358 149 625 0.0 6 622 2 1 150 941 0.0 6 939 2 151 439 0.0 6 438 1 152 658 0.0 6 651 4 0 0 0 0 3 153 4705 0.0 6 4692 10 0 2 0 0 1 154 669 0.0 6 668 1 155 64 0.0 6 64 156 27 0.0 6 27 157 46 0.0 6 46 158 36 0.0 6 36 159 17 0.0 6 17 160 40 0.0 6 40 161 25 0.0 6 25 162 17 0.0 6 17 163 16 0.0 6 16 164 23 0.0 6 23 165 20 0.0 6 20 166 26 0.0 6 26 167 19 0.0 6 19 168 19 0.0 6 19 169 9 0.0 6 9 170 17 0.0 6 17 171 18 0.0 6 18 172 40 0.0 6 40 173 22 0.0 6 22 174 18 0.0 6 18 175 44 0.0 6 44 176 89 0.0 6 89 177 33 0.0 6 33 178 23 0.0 6 23 179 21 0.0 6 21 180 22 0.0 6 21 1 181 51 0.0 6 51 182 31 0.0 6 31 183 33 0.0 6 33 184 40 0.0 6 40 185 48 0.0 6 48 186 95 0.0 6 95 187 34 0.0 6 34 188 30 0.0 6 29 1 189 28 0.0 6 28 190 35 0.0 6 35 191 23 0.0 6 23 192 35 0.0 6 35 193 74 0.0 6 73 0 0 0 0 0 1 194 241 0.0 6 241 195 34 0.0 6 34 196 46 0.0 6 46 197 25 0.0 6 24 1 198 49 0.0 6 49 199 31 0.0 6 31 200 26 0.0 6 26 201 35 0.0 6 35 202 29 0.0 6 29 203 16 0.0 6 15 1 204 33 0.0 6 32 1 205 33 0.0 6 33 206 43 0.0 6 42 1 207 75 0.0 6 74 1 208 31 0.0 6 31 209 69 0.0 6 68 1 210 69 0.0 6 68 1 211 96 0.0 6 95 0 1 212 188 0.0 6 188 213 210 0.0 6 209 0 0 0 0 1 214 87 0.0 6 86 0 1 215 99 0.0 6 98 1 216 139 0.0 6 136 3 217 154 0.0 6 154 218 250 0.0 6 250 219 585 0.0 6 585 220 23 0.0 6 23 221 13 0.0 6 13 222 12 0.0 6 12 223 14 0.0 6 14 224 19 0.0 6 19 225 14 0.0 6 14 226 30 0.0 6 29 0 0 0 0 1 227 22 0.0 6 22 228 35 0.0 6 35 229 39 0.0 6 38 0 0 0 0 1 230 57 0.0 6 56 1 231 64 0.0 6 64 232 44 0.0 6 44 233 42 0.0 6 42 234 56 0.0 6 56 235 51 0.0 6 50 1 236 47 0.0 6 47 237 25 0.0 6 24 1 238 31 0.0 6 31 239 58 0.0 6 58 240 53 0.0 6 52 1 241 34 0.0 6 34 242 91 0.0 6 91 243 25 0.0 6 25 244 58 0.0 6 58 245 49 0.0 6 49 246 36 0.0 6 36 247 62 0.0 6 62 248 82 0.0 6 82 249 63 0.0 6 63 250 77 0.0 6 77 251 87 0.0 6 87 252 122 0.0 6 122 253 27 0.0 6 27 254 21 0.0 6 21 255 25 0.0 6 25 256 22 0.0 6 22 257 14 0.0 6 13 0 1 258 23 0.0 6 23 259 14 0.0 6 13 1 260 88 0.0 6 86 0 0 0 0 0 2 261 22 0.0 6 22 262 5 0.0 6 5 263 15 0.0 6 13 1 1 264 18 0.0 6 18 265 7 0.0 6 7 266 11 0.0 6 11 267 13 0.0 6 13 268 12 0.0 6 12 269 12 0.0 6 12 270 12 0.0 6 12 271 29 0.0 6 29 272 4 0.0 6 4 273 23 0.0 6 23 274 28 0.0 6 28 275 29 0.0 6 29 276 47 0.0 6 46 1 277 61 0.0 6 60 0 0 0 0 0 1 278 33 0.0 6 33 279 194 0.0 6 189 5 280 104 0.0 6 102 1 0 0 0 0 1 281 4 0.0 6 4 284 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1000 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 58 1 0.0 6 0 0 0 0 1 60 2 0.0 6 1 0 1 61 5 0.0 6 1 4 62 257 0.0 6 249 8 63 2 0.0 6 2 70 1 0.0 6 1 71 7 0.0 6 7 72 10 0.0 6 10 73 35 0.0 6 35 74 41 0.0 6 41 75 8 0.0 6 8 76 12 0.0 6 12 77 18 0.0 6 18 78 47 0.0 6 47 79 13 0.0 6 13 80 70 0.0 6 70 81 240 0.0 6 239 1 82 15 0.0 6 15 85 1 0.0 6 1 86 2 0.0 6 2 88 1 0.0 6 1 89 1 0.0 6 1 90 1 0.0 6 1 92 2 0.0 6 2 93 1 0.0 6 1 96 1 0.0 6 1 100 1 0.0 6 1 101 1 0.0 6 1 102 1 0.0 6 1 104 10 0.0 6 10 108 2 0.0 6 2 110 1 0.0 6 1 113 1 0.0 6 1 114 3 0.0 6 3 121 3 0.0 6 3 122 1 0.0 6 1 137 1 0.0 6 1 144 1 0.0 6 1 152 1 0.0 6 1 155 1 0.0 6 1 178 5 0.0 6 5 179 23 0.0 6 23 180 107 0.0 6 107 181 1 0.0 6 1 215 1 0.0 6 1 243 8 0.0 6 8 244 31 0.0 6 31 245 1 0.0 6 1 265 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 18520 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 9 1 0.1 2 1 13 1 0.0 3 1 14 7 0.0 4 5 1 0 1 15 1 0.0 4 1 16 1 0.0 4 1 17 4 0.0 5 3 1 18 3 0.0 5 3 20 13 0.0 6 13 21 4 0.0 6 4 22 2 0.0 6 2 23 2 0.0 6 2 24 1 0.0 6 1 25 1 0.0 6 1 26 10 0.0 6 9 1 27 8 0.0 6 7 0 1 28 4 0.0 6 4 29 7 0.0 6 6 1 30 4 0.0 6 4 31 1 0.0 6 1 32 1 0.0 6 0 1 33 3 0.0 6 3 34 5 0.0 6 5 35 3 0.0 6 3 36 6 0.0 6 5 1 37 8 0.0 6 7 1 38 4 0.0 6 4 40 7 0.0 6 5 2 41 4 0.0 6 4 42 8 0.0 6 6 2 43 7 0.0 6 6 0 0 0 0 1 44 2 0.0 6 2 45 9 0.0 6 9 46 6 0.0 6 5 1 47 5 0.0 6 5 48 10 0.0 6 10 49 10 0.0 6 9 1 50 14 0.0 6 13 1 51 15 0.0 6 15 52 8 0.0 6 8 53 7 0.0 6 7 54 4 0.0 6 3 1 55 15 0.0 6 15 56 6 0.0 6 6 57 7 0.0 6 6 0 1 58 12 0.0 6 12 59 14 0.0 6 12 2 60 4 0.0 6 4 61 9 0.0 6 7 2 62 5 0.0 6 5 63 15 0.0 6 15 64 9 0.0 6 8 1 65 16 0.0 6 15 1 66 13 0.0 6 13 67 36 0.0 6 34 2 68 21 0.0 6 21 69 26 0.0 6 25 1 70 12 0.0 6 12 71 20 0.0 6 19 1 72 18 0.0 6 18 73 20 0.0 6 20 74 9 0.0 6 9 75 19 0.0 6 19 76 15 0.0 6 13 2 77 19 0.0 6 18 1 78 28 0.0 6 27 0 0 0 1 79 13 0.0 6 12 1 80 10 0.0 6 10 81 24 0.0 6 21 2 1 82 15 0.0 6 15 83 20 0.0 6 19 1 84 19 0.0 6 19 85 3 0.0 6 3 86 12 0.0 6 11 1 87 9 0.0 6 9 88 16 0.0 6 16 89 16 0.0 6 16 90 31 0.0 6 30 1 91 17 0.0 6 17 92 18 0.0 6 17 1 93 23 0.0 6 20 3 94 24 0.0 6 24 95 28 0.0 6 28 96 43 0.0 6 42 1 97 31 0.0 6 31 98 40 0.0 6 38 2 99 25 0.0 6 23 1 0 0 0 0 1 100 32 0.0 6 31 1 101 20 0.0 6 20 102 26 0.0 6 25 1 103 18 0.0 6 18 104 58 0.0 6 58 105 40 0.0 6 39 1 106 28 0.0 6 27 1 107 37 0.0 6 36 1 108 21 0.0 6 21 109 50 0.0 6 49 1 110 37 0.0 6 35 2 111 62 0.0 6 60 2 112 53 0.0 6 52 1 113 57 0.0 6 55 2 114 51 0.0 6 51 115 85 0.0 6 84 1 116 52 0.0 6 50 2 117 69 0.0 6 69 118 63 0.0 6 63 119 81 0.0 6 81 120 76 0.0 6 75 1 121 38 0.0 6 38 122 81 0.0 6 78 3 123 100 0.0 6 98 1 1 124 107 0.0 6 104 3 125 112 0.0 6 112 126 122 0.0 6 121 1 127 136 0.0 6 135 1 128 117 0.0 6 112 4 1 129 157 0.0 6 155 2 130 235 0.0 6 228 7 131 136 0.0 6 131 5 132 285 0.0 6 282 3 133 195 0.0 6 194 1 134 160 0.0 6 159 1 135 217 0.0 6 213 4 136 146 0.0 6 145 1 137 207 0.0 6 202 5 138 182 0.0 6 178 4 139 324 0.0 6 321 1 0 2 140 305 0.0 6 302 3 141 668 0.0 6 666 2 142 1019 0.0 6 1011 7 1 143 395 0.0 6 391 4 144 542 0.0 6 533 9 145 629 0.0 6 618 10 1 146 859 0.0 6 850 7 1 0 1 147 729 0.0 6 725 4 148 1090 0.0 6 1067 21 1 0 0 0 1 149 3314 0.0 6 3306 8 150 710 0.0 6 708 2 151 126 0.0 6 126 152 244 0.0 6 236 8 153 126 0.0 6 122 4 154 64 0.0 6 63 1 155 100 0.0 6 99 1 156 108 0.0 6 107 1 157 159 0.0 6 157 2 158 101 0.0 6 99 2 159 89 0.0 6 89 160 61 0.0 6 57 4 161 55 0.0 6 55 162 42 0.0 6 42 163 50 0.0 6 50 164 52 0.0 6 51 1 165 50 0.0 6 50 166 74 0.0 6 71 3 167 79 0.0 6 78 1 168 98 0.0 6 98 169 76 0.0 6 74 2 170 47 0.0 6 47 171 43 0.0 6 43 172 247 0.0 6 246 1 173 62 0.0 6 62 174 46 0.0 6 44 1 0 0 1 175 28 0.0 6 28 176 34 0.0 6 33 1 177 39 0.0 6 39 178 39 0.0 6 39 179 26 0.0 6 26 180 39 0.0 6 39 181 16 0.0 6 16 182 17 0.0 6 17 183 25 0.0 6 25 184 35 0.0 6 35 185 31 0.0 6 30 1 186 19 0.0 6 19 187 34 0.0 6 34 188 26 0.0 6 25 1 189 36 0.0 6 35 0 0 0 1 190 32 0.0 6 30 2 191 18 0.0 6 17 1 192 7 0.0 6 7 193 13 0.0 6 13 194 20 0.0 6 18 2 195 13 0.0 6 12 1 196 23 0.0 6 23 197 10 0.0 6 10 198 14 0.0 6 13 1 199 20 0.0 6 20 200 10 0.0 6 10 201 10 0.0 6 10 202 12 0.0 6 11 1 203 4 0.0 6 4 204 14 0.0 6 14 205 14 0.0 6 12 2 206 10 0.0 6 10 207 10 0.0 6 9 1 208 26 0.0 6 25 0 0 0 1 209 6 0.0 6 4 0 0 0 1 1 210 13 0.0 6 13 211 22 0.0 6 22 212 4 0.0 6 4 213 7 0.0 6 6 1 214 15 0.0 6 15 215 4 0.0 6 4 216 4 0.0 6 4 217 1 0.0 6 1 218 4 0.0 6 4 219 3 0.0 6 2 0 0 0 0 1 220 3 0.0 6 3 221 7 0.0 6 7 222 1 0.0 6 1 223 13 0.0 6 13 224 4 0.0 6 4 225 13 0.0 6 13 226 2 0.0 6 2 227 7 0.0 6 7 228 2 0.0 6 2 230 7 0.0 6 7 231 5 0.0 6 4 1 232 10 0.0 6 9 0 0 0 1 233 7 0.0 6 7 234 4 0.0 6 4 235 11 0.0 6 11 236 1 0.0 6 1 237 2 0.0 6 2 238 1 0.0 6 1 239 1 0.0 6 1 241 2 0.0 6 2 242 10 0.0 6 9 0 0 0 1 243 4 0.0 6 4 244 4 0.0 6 4 245 8 0.0 6 7 1 246 8 0.0 6 8 247 9 0.0 6 9 248 1 0.0 6 1 249 2 0.0 6 2 251 1 0.0 6 1 252 1 0.0 6 1 278 1 0.0 6 1 279 3 0.0 6 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 833 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 71 1 0.0 6 1 72 13 0.0 6 13 73 6 0.0 6 6 74 48 0.0 6 48 75 56 0.0 6 56 76 11 0.0 6 10 1 77 20 0.0 6 20 78 34 0.0 6 33 1 79 49 0.0 6 49 80 13 0.0 6 13 81 45 0.0 6 45 82 389 0.0 6 388 1 83 12 0.0 6 12 85 6 0.0 6 3 0 0 1 2 86 6 0.0 6 5 0 0 0 1 87 2 0.0 6 2 89 1 0.0 6 1 90 1 0.0 6 1 91 1 0.0 6 1 92 2 0.0 6 2 93 1 0.0 6 1 96 3 0.0 6 3 98 1 0.0 6 1 101 1 0.0 6 1 103 1 0.0 6 1 104 6 0.0 6 6 105 5 0.0 6 5 106 2 0.0 6 2 108 1 0.0 6 1 109 1 0.0 6 1 110 1 0.0 6 1 111 1 0.0 6 1 113 1 0.0 6 1 114 1 0.0 6 1 115 5 0.0 6 5 118 1 0.0 6 1 120 1 0.0 6 1 122 3 0.0 6 3 123 2 0.0 6 2 124 1 0.0 6 1 127 1 0.0 6 1 132 1 0.0 6 1 141 1 0.0 6 1 159 1 0.0 6 1 164 1 0.0 6 1 172 1 0.0 6 1 174 1 0.0 6 1 176 1 0.0 6 1 177 1 0.0 6 1 179 2 0.0 6 2 180 11 0.0 6 11 181 54 0.0 6 54 182 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_11_210_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_11_437_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_11_210_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_11_437_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.25 s (47 µs/read; 1.28 M reads/minute). === Summary === Total read pairs processed: 47,821 Read 1 with adapter: 47,818 (100.0%) Read 2 with adapter: 47,804 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 20 (0.0%) Pairs written (passing filters): 47,801 (100.0%) Total basepairs processed: 23,856,387 bp Read 1: 13,769,146 bp Read 2: 10,087,241 bp Total written (filtered): 2,869,191 bp (12.0%) Read 1: 1,482,568 bp Read 2: 1,386,623 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 47802 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 186.8 1 0 1 6 2 11.7 1 0 0 2 10 1 0.0 3 1 16 1 0.0 4 1 17 1 0.0 5 1 20 8 0.0 6 8 21 4 0.0 6 4 22 4 0.0 6 4 24 1 0.0 6 1 25 9 0.0 6 9 26 5 0.0 6 5 27 5 0.0 6 5 28 2 0.0 6 2 29 1 0.0 6 1 30 3 0.0 6 1 2 33 5 0.0 6 4 1 35 3 0.0 6 0 2 0 0 0 0 1 38 2 0.0 6 2 40 1 0.0 6 1 41 1 0.0 6 1 42 1 0.0 6 1 44 5 0.0 6 5 45 2 0.0 6 2 46 1 0.0 6 1 47 1 0.0 6 1 49 1 0.0 6 0 0 0 0 0 0 1 50 1 0.0 6 1 51 1 0.0 6 1 52 1 0.0 6 1 53 3 0.0 6 2 1 54 2 0.0 6 2 56 1 0.0 6 0 1 57 1 0.0 6 1 59 2 0.0 6 2 60 3 0.0 6 3 62 1 0.0 6 1 63 1 0.0 6 1 66 3 0.0 6 2 1 67 2 0.0 6 2 68 1 0.0 6 1 71 1 0.0 6 1 72 2 0.0 6 2 77 1 0.0 6 1 78 6 0.0 6 6 79 1 0.0 6 0 1 80 1 0.0 6 1 81 2 0.0 6 2 86 11 0.0 6 11 87 5 0.0 6 5 90 1 0.0 6 1 92 2 0.0 6 2 93 1 0.0 6 0 1 96 2 0.0 6 2 97 2 0.0 6 2 98 3 0.0 6 3 100 2 0.0 6 2 101 3 0.0 6 3 102 6 0.0 6 3 3 103 4 0.0 6 4 104 1 0.0 6 1 105 2 0.0 6 1 0 1 106 1 0.0 6 1 107 4 0.0 6 2 2 108 3 0.0 6 2 1 109 2 0.0 6 2 111 2 0.0 6 2 113 1 0.0 6 1 114 1 0.0 6 1 115 4 0.0 6 3 1 116 2 0.0 6 2 117 1 0.0 6 1 118 2 0.0 6 2 119 3 0.0 6 2 1 120 2 0.0 6 1 1 121 7 0.0 6 7 122 11 0.0 6 11 123 11 0.0 6 10 1 124 3 0.0 6 3 125 11 0.0 6 11 126 3 0.0 6 3 127 3 0.0 6 3 128 5 0.0 6 5 129 2 0.0 6 2 130 1 0.0 6 1 131 2 0.0 6 2 132 5 0.0 6 5 133 6 0.0 6 6 134 12 0.0 6 12 135 10 0.0 6 9 1 136 8 0.0 6 8 137 26 0.0 6 23 3 138 14 0.0 6 14 139 12 0.0 6 12 140 20 0.0 6 18 2 141 7 0.0 6 6 1 142 13 0.0 6 11 1 1 143 22 0.0 6 21 1 144 11 0.0 6 11 145 14 0.0 6 13 1 146 23 0.0 6 23 147 38 0.0 6 36 2 148 17 0.0 6 16 1 149 21 0.0 6 20 1 150 41 0.0 6 40 1 151 32 0.0 6 32 152 47 0.0 6 47 153 26 0.0 6 25 1 154 31 0.0 6 30 0 1 155 11 0.0 6 10 0 1 156 13 0.0 6 12 1 157 9 0.0 6 9 158 18 0.0 6 18 159 25 0.0 6 23 2 160 13 0.0 6 13 161 34 0.0 6 34 162 27 0.0 6 25 2 163 53 0.0 6 52 1 164 34 0.0 6 34 165 31 0.0 6 28 3 166 23 0.0 6 21 2 167 17 0.0 6 16 0 1 168 19 0.0 6 18 1 169 30 0.0 6 30 170 28 0.0 6 27 1 171 19 0.0 6 19 172 33 0.0 6 33 173 65 0.0 6 61 4 174 40 0.0 6 39 1 175 43 0.0 6 42 1 176 41 0.0 6 40 1 177 46 0.0 6 44 2 178 37 0.0 6 36 1 179 35 0.0 6 32 2 1 180 32 0.0 6 31 1 181 47 0.0 6 46 1 182 45 0.0 6 43 2 183 38 0.0 6 35 3 184 57 0.0 6 55 2 185 92 0.0 6 89 3 186 117 0.0 6 116 1 187 60 0.0 6 55 5 188 64 0.0 6 64 189 54 0.0 6 50 4 190 53 0.0 6 51 2 191 83 0.0 6 82 1 192 78 0.0 6 75 3 193 104 0.0 6 103 1 194 103 0.0 6 96 6 1 195 104 0.0 6 100 4 196 151 0.0 6 149 2 197 115 0.0 6 112 3 198 152 0.0 6 148 4 199 122 0.0 6 119 3 200 162 0.0 6 156 6 201 154 0.0 6 150 4 202 152 0.0 6 150 2 203 185 0.0 6 183 2 204 154 0.0 6 151 3 205 150 0.0 6 150 206 172 0.0 6 168 4 207 276 0.0 6 267 8 1 208 193 0.0 6 188 5 209 240 0.0 6 233 6 1 210 122 0.0 6 116 6 211 90 0.0 6 87 3 212 87 0.0 6 83 4 213 172 0.0 6 170 2 214 141 0.0 6 140 1 215 133 0.0 6 126 6 1 216 188 0.0 6 181 7 217 240 0.0 6 233 7 218 168 0.0 6 166 2 219 160 0.0 6 156 3 1 220 152 0.0 6 145 7 221 146 0.0 6 142 4 222 195 0.0 6 188 7 223 90 0.0 6 88 2 224 81 0.0 6 78 2 0 1 225 172 0.0 6 168 4 226 470 0.0 6 462 8 227 374 0.0 6 368 6 228 336 0.0 6 329 6 1 229 246 0.0 6 241 4 1 230 291 0.0 6 283 6 2 231 232 0.0 6 227 5 232 297 0.0 6 291 5 1 233 353 0.0 6 342 11 234 358 0.0 6 349 9 235 198 0.0 6 194 4 236 104 0.0 6 100 4 237 125 0.0 6 122 3 238 182 0.0 6 180 2 239 195 0.0 6 187 7 0 0 1 240 299 0.0 6 296 3 241 455 0.0 6 442 12 1 242 413 0.0 6 402 11 243 323 0.0 6 319 4 244 260 0.0 6 251 9 245 437 0.0 6 426 11 246 131 0.0 6 128 3 247 211 0.0 6 210 1 248 128 0.0 6 124 4 249 358 0.0 6 350 8 250 201 0.0 6 195 6 251 280 0.0 6 273 7 252 287 0.0 6 279 8 253 237 0.0 6 226 11 254 549 0.0 6 537 12 255 251 0.0 6 247 4 256 224 0.0 6 220 4 257 244 0.0 6 235 9 258 300 0.0 6 295 5 259 451 0.0 6 438 13 260 629 0.0 6 622 7 261 202 0.0 6 192 10 262 399 0.0 6 386 12 1 263 1047 0.0 6 1033 13 1 264 409 0.0 6 399 9 1 265 392 0.0 6 382 10 266 238 0.0 6 230 8 267 400 0.0 6 386 13 1 268 353 0.0 6 349 4 269 464 0.0 6 460 4 270 190 0.0 6 180 6 4 271 550 0.0 6 528 22 272 1041 0.0 6 1018 22 1 273 558 0.0 6 549 8 0 0 0 0 1 274 585 0.0 6 567 17 1 275 979 0.0 6 961 18 276 1324 0.0 6 1302 22 277 1217 0.0 6 1187 29 1 278 743 0.0 6 720 21 2 279 272 0.0 6 249 19 3 1 280 18614 0.0 6 18178 432 4 281 104 0.0 6 88 13 3 282 2 0.0 6 1 0 0 1 283 3 0.0 6 1 1 1 284 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 16 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 76 1 0.0 6 1 101 1 0.0 6 1 104 1 0.0 6 1 105 1 0.0 6 1 118 1 0.0 6 1 119 2 0.0 6 2 121 1 0.0 6 1 123 1 0.0 6 1 150 1 0.0 6 1 151 2 0.0 6 2 173 1 0.0 6 1 177 3 0.0 6 3 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 47788 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 747.2 0 1 7 3 2.9 2 3 11 2 0.0 3 1 1 12 2 0.0 3 1 1 13 3 0.0 3 2 1 14 6 0.0 4 6 15 3 0.0 4 2 0 1 16 5 0.0 4 5 17 9 0.0 5 8 1 18 4 0.0 5 3 1 19 23 0.0 5 21 1 1 20 59 0.0 6 56 3 21 45 0.0 6 39 6 22 28 0.0 6 28 23 14 0.0 6 14 24 10 0.0 6 8 2 25 17 0.0 6 16 1 26 20 0.0 6 18 1 1 27 9 0.0 6 8 1 28 5 0.0 6 4 0 1 29 9 0.0 6 9 30 8 0.0 6 8 31 12 0.0 6 9 3 32 7 0.0 6 6 1 33 8 0.0 6 8 34 11 0.0 6 10 1 35 4 0.0 6 4 36 5 0.0 6 4 1 37 11 0.0 6 11 38 13 0.0 6 12 1 39 9 0.0 6 8 1 40 8 0.0 6 7 1 41 2 0.0 6 2 42 4 0.0 6 4 43 5 0.0 6 5 44 4 0.0 6 4 45 7 0.0 6 4 3 46 14 0.0 6 13 1 47 8 0.0 6 7 1 48 18 0.0 6 17 1 49 13 0.0 6 12 0 1 50 19 0.0 6 17 2 51 10 0.0 6 8 2 52 14 0.0 6 12 2 53 17 0.0 6 13 4 54 8 0.0 6 7 0 1 55 19 0.0 6 18 1 56 19 0.0 6 17 2 57 25 0.0 6 24 1 58 14 0.0 6 13 1 59 15 0.0 6 14 0 1 60 16 0.0 6 14 1 0 0 0 1 61 9 0.0 6 8 1 62 14 0.0 6 13 1 63 8 0.0 6 7 0 1 64 8 0.0 6 8 65 9 0.0 6 9 66 8 0.0 6 5 3 67 14 0.0 6 12 2 68 17 0.0 6 16 1 69 17 0.0 6 17 70 65 0.0 6 61 4 71 18 0.0 6 16 2 72 65 0.0 6 61 4 73 11 0.0 6 10 1 74 13 0.0 6 13 75 14 0.0 6 12 1 1 76 11 0.0 6 10 1 77 8 0.0 6 8 78 22 0.0 6 20 2 79 11 0.0 6 10 1 80 14 0.0 6 14 81 13 0.0 6 13 82 16 0.0 6 15 1 83 23 0.0 6 20 3 84 31 0.0 6 30 0 0 0 1 85 26 0.0 6 26 86 34 0.0 6 31 2 0 0 1 87 39 0.0 6 32 6 1 88 20 0.0 6 15 2 3 89 16 0.0 6 16 90 30 0.0 6 27 2 1 91 18 0.0 6 18 92 20 0.0 6 20 93 25 0.0 6 22 2 0 0 1 94 57 0.0 6 54 2 1 95 17 0.0 6 15 2 96 40 0.0 6 38 2 97 27 0.0 6 24 3 98 22 0.0 6 21 1 99 42 0.0 6 34 7 0 1 100 31 0.0 6 30 1 101 30 0.0 6 29 1 102 66 0.0 6 62 4 103 60 0.0 6 57 1 2 104 54 0.0 6 50 1 3 105 53 0.0 6 52 1 106 31 0.0 6 29 2 107 55 0.0 6 51 4 108 31 0.0 6 24 7 109 21 0.0 6 19 2 110 29 0.0 6 29 111 40 0.0 6 35 4 0 0 1 112 44 0.0 6 43 1 113 22 0.0 6 21 1 114 28 0.0 6 26 2 115 33 0.0 6 31 2 116 40 0.0 6 39 1 117 30 0.0 6 29 1 118 38 0.0 6 35 2 0 0 1 119 42 0.0 6 40 1 0 0 1 120 77 0.0 6 76 1 121 40 0.0 6 36 4 122 72 0.0 6 64 7 1 123 84 0.0 6 80 2 0 1 1 124 126 0.0 6 121 5 125 48 0.0 6 45 3 126 41 0.0 6 40 1 127 55 0.0 6 53 2 128 71 0.0 6 71 129 56 0.0 6 52 4 130 50 0.0 6 49 1 131 66 0.0 6 65 1 132 140 0.0 6 132 7 1 133 103 0.0 6 99 4 134 147 0.0 6 140 5 1 1 135 243 0.0 6 239 4 136 105 0.0 6 101 4 137 161 0.0 6 154 6 0 0 1 138 118 0.0 6 114 4 139 98 0.0 6 95 3 140 94 0.0 6 91 1 1 0 1 141 173 0.0 6 169 3 1 142 247 0.0 6 236 9 1 0 0 0 1 143 178 0.0 6 171 5 2 144 261 0.0 6 257 4 145 145 0.0 6 142 3 146 138 0.0 6 133 4 0 0 0 1 147 73 0.0 6 70 2 0 1 148 134 0.0 6 127 6 0 0 1 149 242 0.0 6 233 8 0 0 1 150 324 0.0 6 314 8 1 0 1 151 177 0.0 6 163 12 0 0 2 152 435 0.0 6 415 19 1 153 337 0.0 6 323 12 2 154 286 0.0 6 279 5 1 0 1 155 385 0.0 6 379 4 1 1 156 311 0.0 6 298 11 1 0 1 157 374 0.0 6 365 9 158 440 0.0 6 432 7 1 159 163 0.0 6 152 11 160 434 0.0 6 424 9 1 161 331 0.0 6 316 14 1 162 256 0.0 6 248 7 0 0 1 163 350 0.0 6 339 8 1 0 2 164 304 0.0 6 293 10 0 1 165 383 0.0 6 372 11 166 471 0.0 6 462 8 1 167 184 0.0 6 170 12 1 0 0 0 1 168 603 0.0 6 589 14 169 138 0.0 6 134 4 170 190 0.0 6 184 6 171 492 0.0 6 479 12 1 172 166 0.0 6 164 2 173 223 0.0 6 216 6 1 174 305 0.0 6 291 14 175 658 0.0 6 642 16 176 378 0.0 6 371 7 177 653 0.0 6 626 26 1 178 374 0.0 6 356 17 1 179 556 0.0 6 542 12 2 180 497 0.0 6 485 10 1 0 1 181 844 0.0 6 817 25 0 0 2 182 296 0.0 6 283 13 183 612 0.0 6 589 22 0 0 1 184 189 0.0 6 180 8 1 185 203 0.0 6 190 12 1 186 797 0.0 6 766 29 0 1 1 187 991 0.0 6 965 24 2 188 1137 0.0 6 1097 37 0 1 1 1 189 986 0.0 6 959 25 1 1 190 1367 0.0 6 1328 35 2 0 1 1 191 1150 0.0 6 1120 27 2 0 1 192 1013 0.0 6 979 32 0 0 2 193 607 0.0 6 591 15 1 194 922 0.0 6 899 19 4 195 935 0.0 6 903 30 2 196 2770 0.0 6 2717 50 2 0 1 197 551 0.0 6 537 14 198 1251 0.0 6 1208 43 199 2980 0.0 6 2901 76 1 0 2 200 568 0.0 6 550 18 201 1023 0.0 6 1007 13 2 0 1 202 533 0.0 6 517 15 1 203 447 0.0 6 442 4 1 204 530 0.0 6 525 5 205 276 0.0 6 272 4 206 205 0.0 6 197 8 207 131 0.0 6 127 4 208 266 0.0 6 256 10 209 239 0.0 6 235 4 210 211 0.0 6 208 3 211 379 0.0 6 367 10 1 0 0 1 212 386 0.0 6 370 14 0 1 1 213 60 0.0 6 59 1 214 132 0.0 6 129 3 215 139 0.0 6 137 2 216 547 0.0 6 538 9 217 522 0.0 6 506 15 1 218 219 0.0 6 213 6 219 292 0.0 6 285 7 220 184 0.0 6 181 3 221 358 0.0 6 346 12 222 732 0.0 6 715 17 223 659 0.0 6 648 11 224 80 0.0 6 76 4 225 239 0.0 6 237 2 226 105 0.0 6 105 227 164 0.0 6 159 5 228 108 0.0 6 106 1 1 229 134 0.0 6 128 6 230 64 0.0 6 61 3 231 87 0.0 6 83 4 232 168 0.0 6 164 4 233 156 0.0 6 153 3 234 56 0.0 6 54 2 235 60 0.0 6 55 5 236 30 0.0 6 30 237 41 0.0 6 40 1 238 15 0.0 6 14 0 1 239 13 0.0 6 13 240 36 0.0 6 35 1 241 47 0.0 6 46 1 242 107 0.0 6 105 2 243 75 0.0 6 73 2 244 145 0.0 6 144 1 245 32 0.0 6 31 1 246 20 0.0 6 20 247 6 0.0 6 6 248 12 0.0 6 12 249 18 0.0 6 18 250 21 0.0 6 21 251 20 0.0 6 20 252 4 0.0 6 4 253 6 0.0 6 6 254 13 0.0 6 13 256 3 0.0 6 3 280 1 0.0 6 1 281 11 0.0 6 10 0 1 282 14 0.0 6 13 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 16 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 75 1 0.0 6 1 78 1 0.0 6 1 79 1 0.0 6 1 82 1 0.0 6 1 92 1 0.0 6 1 98 1 0.0 6 1 100 1 0.0 6 1 120 1 0.0 6 1 134 1 0.0 6 1 149 1 0.0 6 1 153 1 0.0 6 1 172 1 0.0 6 1 174 2 0.0 6 2 175 1 0.0 6 1 176 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_12_211_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_12_438_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_12_211_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_12_438_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.88 s (48 µs/read; 1.24 M reads/minute). === Summary === Total read pairs processed: 59,553 Read 1 with adapter: 59,544 (100.0%) Read 2 with adapter: 59,483 (99.9%) == Read fate breakdown == Pairs that were too short: 16 (0.0%) Pairs discarded as untrimmed: 79 (0.1%) Pairs written (passing filters): 59,458 (99.8%) Total basepairs processed: 25,598,650 bp Read 1: 14,529,852 bp Read 2: 11,068,798 bp Total written (filtered): 3,771,157 bp (14.7%) Read 1: 1,902,658 bp Read 2: 1,868,499 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 58240 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 930.5 0 0 1 5 1 58.2 1 1 6 1 14.5 1 0 0 1 7 2 3.6 2 2 9 1 0.2 2 1 10 1 0.1 3 1 15 1 0.0 4 1 16 2 0.0 4 2 17 3 0.0 5 3 20 71 0.0 6 69 2 21 28 0.0 6 26 2 22 34 0.0 6 34 23 27 0.0 6 25 2 24 17 0.0 6 16 1 25 12 0.0 6 9 2 0 0 0 1 26 7 0.0 6 6 1 27 4 0.0 6 4 28 7 0.0 6 7 29 6 0.0 6 6 30 6 0.0 6 6 31 5 0.0 6 5 32 3 0.0 6 3 33 3 0.0 6 3 34 3 0.0 6 3 35 2 0.0 6 2 36 5 0.0 6 5 37 2 0.0 6 2 38 5 0.0 6 5 39 8 0.0 6 8 40 6 0.0 6 6 41 4 0.0 6 4 42 6 0.0 6 6 43 4 0.0 6 4 44 1 0.0 6 1 45 3 0.0 6 3 46 3 0.0 6 3 47 4 0.0 6 4 48 3 0.0 6 3 49 4 0.0 6 4 50 9 0.0 6 9 51 6 0.0 6 6 52 5 0.0 6 5 53 7 0.0 6 7 54 5 0.0 6 5 55 3 0.0 6 3 56 3 0.0 6 3 57 3 0.0 6 3 58 2 0.0 6 1 1 59 4 0.0 6 3 0 0 0 0 1 60 7 0.0 6 7 61 4 0.0 6 4 63 6 0.0 6 5 1 64 4 0.0 6 4 65 6 0.0 6 6 66 9 0.0 6 9 67 7 0.0 6 7 68 4 0.0 6 4 69 3 0.0 6 3 70 7 0.0 6 7 71 8 0.0 6 8 72 8 0.0 6 8 73 5 0.0 6 5 74 4 0.0 6 4 75 8 0.0 6 8 76 3 0.0 6 3 77 6 0.0 6 6 78 6 0.0 6 6 79 8 0.0 6 6 2 80 2 0.0 6 2 81 11 0.0 6 9 1 0 0 0 0 1 82 7 0.0 6 6 1 83 9 0.0 6 9 84 12 0.0 6 12 85 10 0.0 6 10 86 6 0.0 6 5 1 87 7 0.0 6 7 88 6 0.0 6 4 1 0 0 0 0 1 89 11 0.0 6 11 90 8 0.0 6 8 91 2 0.0 6 2 92 5 0.0 6 5 93 7 0.0 6 7 94 8 0.0 6 8 95 14 0.0 6 14 96 5 0.0 6 5 97 6 0.0 6 4 2 98 12 0.0 6 11 0 0 0 0 0 1 99 6 0.0 6 6 100 5 0.0 6 4 1 101 11 0.0 6 9 2 102 13 0.0 6 12 1 103 12 0.0 6 12 104 13 0.0 6 12 1 105 14 0.0 6 14 106 17 0.0 6 16 0 0 0 0 0 1 107 20 0.0 6 19 1 108 12 0.0 6 11 1 109 13 0.0 6 13 110 11 0.0 6 10 1 111 12 0.0 6 12 112 12 0.0 6 12 113 14 0.0 6 13 1 114 14 0.0 6 14 115 12 0.0 6 11 1 116 25 0.0 6 24 1 117 11 0.0 6 11 118 14 0.0 6 14 119 12 0.0 6 12 120 16 0.0 6 15 1 121 11 0.0 6 11 122 19 0.0 6 18 1 123 16 0.0 6 16 124 22 0.0 6 20 2 125 15 0.0 6 15 126 16 0.0 6 16 127 17 0.0 6 16 1 128 22 0.0 6 22 129 18 0.0 6 17 1 130 27 0.0 6 27 131 31 0.0 6 31 132 30 0.0 6 30 133 16 0.0 6 16 134 15 0.0 6 15 135 32 0.0 6 32 136 37 0.0 6 37 137 26 0.0 6 25 1 138 28 0.0 6 27 1 139 30 0.0 6 30 140 38 0.0 6 37 0 1 141 46 0.0 6 46 142 135 0.0 6 135 143 223 0.0 6 223 144 343 0.0 6 342 0 0 0 1 145 198 0.0 6 198 146 222 0.0 6 222 147 315 0.0 6 313 2 148 224 0.0 6 221 3 149 282 0.0 6 282 150 294 0.0 6 291 2 1 151 303 0.0 6 302 1 152 206 0.0 6 204 2 153 229 0.0 6 228 1 154 144 0.0 6 143 1 155 197 0.0 6 196 0 1 156 263 0.0 6 261 2 157 224 0.0 6 223 1 158 177 0.0 6 177 159 178 0.0 6 177 1 160 228 0.0 6 228 161 255 0.0 6 255 162 250 0.0 6 246 4 163 226 0.0 6 226 164 313 0.0 6 312 1 165 265 0.0 6 264 1 166 201 0.0 6 200 1 167 224 0.0 6 222 1 1 168 246 0.0 6 243 2 0 0 0 0 1 169 279 0.0 6 278 1 170 244 0.0 6 242 2 171 234 0.0 6 233 1 172 250 0.0 6 249 0 0 0 0 0 1 173 263 0.0 6 261 2 174 329 0.0 6 327 2 175 333 0.0 6 329 4 176 228 0.0 6 228 177 292 0.0 6 292 178 230 0.0 6 229 1 179 306 0.0 6 304 2 180 742 0.0 6 740 0 0 0 0 1 1 181 312 0.0 6 311 1 182 251 0.0 6 250 1 183 284 0.0 6 284 184 265 0.0 6 261 3 1 185 291 0.0 6 291 186 344 0.0 6 338 5 0 1 187 335 0.0 6 332 2 1 188 236 0.0 6 233 3 189 281 0.0 6 279 2 190 277 0.0 6 274 3 191 286 0.0 6 285 1 192 356 0.0 6 355 1 193 378 0.0 6 375 3 194 407 0.0 6 406 1 195 525 0.0 6 523 2 196 456 0.0 6 450 4 1 1 197 588 0.0 6 586 2 198 740 0.0 6 733 7 199 609 0.0 6 606 3 200 501 0.0 6 495 5 0 0 1 201 492 0.0 6 488 4 202 486 0.0 6 478 7 0 1 203 489 0.0 6 487 0 1 1 204 739 0.0 6 736 2 0 0 1 205 506 0.0 6 501 4 0 0 0 0 1 206 595 0.0 6 589 6 207 835 0.0 6 829 5 0 0 1 208 615 0.0 6 610 4 0 1 209 707 0.0 6 705 2 210 636 0.0 6 631 5 211 541 0.0 6 538 3 212 632 0.0 6 628 4 213 718 0.0 6 713 4 1 214 608 0.0 6 607 1 215 787 0.0 6 781 4 0 0 1 1 216 961 0.0 6 955 6 217 736 0.0 6 735 1 218 759 0.0 6 754 5 219 819 0.0 6 816 2 0 1 220 710 0.0 6 706 4 221 569 0.0 6 565 1 0 1 0 1 1 222 644 0.0 6 639 4 0 0 0 0 1 223 608 0.0 6 601 6 0 1 224 634 0.0 6 628 6 225 728 0.0 6 724 4 226 811 0.0 6 805 6 227 718 0.0 6 713 4 1 228 670 0.0 6 665 4 0 1 229 615 0.0 6 613 1 1 230 647 0.0 6 642 4 1 231 650 0.0 6 647 3 232 579 0.0 6 578 1 233 631 0.0 6 629 2 234 571 0.0 6 569 2 235 505 0.0 6 503 2 236 442 0.0 6 441 1 237 571 0.0 6 568 2 1 238 471 0.0 6 469 1 1 239 499 0.0 6 495 4 240 534 0.0 6 532 1 0 0 1 241 470 0.0 6 469 1 242 439 0.0 6 437 2 243 447 0.0 6 443 4 244 420 0.0 6 418 1 0 0 0 1 245 393 0.0 6 392 1 246 392 0.0 6 390 2 247 330 0.0 6 329 1 248 299 0.0 6 293 6 249 348 0.0 6 342 5 0 0 1 250 301 0.0 6 298 3 251 311 0.0 6 308 2 1 252 313 0.0 6 313 253 276 0.0 6 273 3 254 302 0.0 6 300 2 255 303 0.0 6 300 2 0 1 256 254 0.0 6 251 3 257 263 0.0 6 261 2 258 267 0.0 6 265 2 259 252 0.0 6 251 1 260 302 0.0 6 300 2 261 269 0.0 6 269 262 202 0.0 6 200 2 263 224 0.0 6 222 2 264 208 0.0 6 207 1 265 193 0.0 6 191 2 266 204 0.0 6 202 2 267 213 0.0 6 213 268 191 0.0 6 190 1 269 193 0.0 6 192 1 270 199 0.0 6 197 2 271 163 0.0 6 162 1 272 133 0.0 6 133 273 139 0.0 6 139 274 189 0.0 6 188 1 275 179 0.0 6 178 1 276 207 0.0 6 205 2 277 299 0.0 6 295 4 278 322 0.0 6 320 2 279 1152 0.0 6 1146 4 1 0 0 0 1 280 1976 0.0 6 1965 10 0 1 281 3 0.0 6 3 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1304 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 62 23 0.0 6 21 1 1 70 1 0.0 6 1 71 2 0.0 6 2 72 9 0.0 6 9 73 11 0.0 6 11 74 6 0.0 6 6 75 8 0.0 6 8 76 8 0.0 6 8 77 11 0.0 6 11 78 7 0.0 6 7 79 10 0.0 6 10 80 5 0.0 6 5 81 5 0.0 6 5 82 1 0.0 6 1 83 4 0.0 6 4 84 5 0.0 6 5 85 9 0.0 6 9 86 3 0.0 6 3 87 2 0.0 6 2 88 4 0.0 6 4 89 9 0.0 6 9 90 9 0.0 6 9 91 11 0.0 6 11 92 11 0.0 6 11 93 5 0.0 6 5 94 3 0.0 6 3 95 5 0.0 6 5 96 7 0.0 6 7 97 6 0.0 6 6 98 7 0.0 6 7 99 3 0.0 6 3 100 2 0.0 6 2 101 7 0.0 6 7 102 6 0.0 6 6 103 3 0.0 6 3 104 10 0.0 6 10 105 4 0.0 6 4 107 6 0.0 6 6 108 11 0.0 6 11 109 21 0.0 6 21 110 4 0.0 6 4 111 5 0.0 6 5 112 6 0.0 6 6 113 3 0.0 6 2 0 0 0 0 0 1 114 4 0.0 6 4 115 5 0.0 6 5 116 7 0.0 6 7 117 5 0.0 6 5 118 3 0.0 6 3 119 3 0.0 6 3 120 2 0.0 6 2 121 9 0.0 6 9 122 5 0.0 6 5 123 6 0.0 6 6 124 7 0.0 6 7 125 1 0.0 6 1 126 7 0.0 6 7 127 3 0.0 6 3 128 1 0.0 6 1 129 5 0.0 6 5 130 8 0.0 6 8 131 3 0.0 6 3 132 9 0.0 6 9 133 7 0.0 6 7 134 2 0.0 6 2 135 6 0.0 6 6 136 6 0.0 6 6 137 8 0.0 6 8 138 8 0.0 6 8 139 9 0.0 6 9 140 3 0.0 6 3 141 5 0.0 6 5 142 2 0.0 6 2 143 7 0.0 6 7 144 5 0.0 6 5 145 6 0.0 6 6 146 3 0.0 6 3 147 10 0.0 6 10 148 10 0.0 6 10 149 9 0.0 6 9 150 9 0.0 6 9 151 14 0.0 6 14 152 8 0.0 6 8 153 8 0.0 6 8 154 10 0.0 6 10 155 10 0.0 6 10 156 8 0.0 6 8 157 12 0.0 6 12 158 10 0.0 6 10 159 7 0.0 6 7 160 10 0.0 6 10 161 6 0.0 6 6 162 12 0.0 6 12 163 5 0.0 6 5 164 8 0.0 6 8 165 7 0.0 6 7 166 13 0.0 6 13 167 8 0.0 6 8 168 6 0.0 6 6 169 7 0.0 6 7 170 5 0.0 6 5 171 8 0.0 6 8 172 7 0.0 6 7 173 8 0.0 6 8 174 4 0.0 6 4 175 7 0.0 6 7 176 6 0.0 6 6 177 3 0.0 6 3 178 11 0.0 6 11 179 5 0.0 6 5 180 8 0.0 6 8 181 5 0.0 6 5 182 6 0.0 6 6 183 12 0.0 6 11 1 184 14 0.0 6 14 185 7 0.0 6 7 186 10 0.0 6 10 187 8 0.0 6 8 188 17 0.0 6 17 189 17 0.0 6 17 190 11 0.0 6 11 191 6 0.0 6 6 192 15 0.0 6 15 193 10 0.0 6 10 194 7 0.0 6 7 195 6 0.0 6 6 196 7 0.0 6 7 197 7 0.0 6 7 198 6 0.0 6 6 199 4 0.0 6 4 200 6 0.0 6 6 201 4 0.0 6 4 202 12 0.0 6 12 203 12 0.0 6 12 204 5 0.0 6 5 205 5 0.0 6 5 206 4 0.0 6 4 207 4 0.0 6 4 208 4 0.0 6 4 209 4 0.0 6 4 210 14 0.0 6 14 211 14 0.0 6 14 212 4 0.0 6 4 213 10 0.0 6 10 214 9 0.0 6 9 215 7 0.0 6 7 216 10 0.0 6 10 217 7 0.0 6 7 218 9 0.0 6 9 219 4 0.0 6 4 220 5 0.0 6 5 221 4 0.0 6 4 222 6 0.0 6 6 223 6 0.0 6 6 224 8 0.0 6 8 225 8 0.0 6 8 226 5 0.0 6 5 227 5 0.0 6 5 228 6 0.0 6 6 229 7 0.0 6 7 230 7 0.0 6 7 231 1 0.0 6 1 232 2 0.0 6 2 233 2 0.0 6 2 234 10 0.0 6 10 235 10 0.0 6 10 236 8 0.0 6 8 237 3 0.0 6 3 238 4 0.0 6 4 239 2 0.0 6 2 240 4 0.0 6 4 241 5 0.0 6 5 242 7 0.0 6 7 243 3 0.0 6 3 244 3 0.0 6 3 245 4 0.0 6 4 246 2 0.0 6 2 247 3 0.0 6 3 248 4 0.0 6 4 249 2 0.0 6 2 250 2 0.0 6 2 251 3 0.0 6 3 252 3 0.0 6 3 253 7 0.0 6 7 254 2 0.0 6 2 255 5 0.0 6 5 256 4 0.0 6 4 257 6 0.0 6 6 258 3 0.0 6 3 259 2 0.0 6 2 260 2 0.0 6 2 261 2 0.0 6 2 262 2 0.0 6 2 263 1 0.0 6 1 265 1 0.0 6 1 266 3 0.0 6 3 267 3 0.0 6 3 268 2 0.0 6 2 269 1 0.0 6 1 271 2 0.0 6 2 272 2 0.0 6 2 273 1 0.0 6 1 274 1 0.0 6 1 276 6 0.0 6 6 277 4 0.0 6 4 279 1 0.0 6 1 280 2 0.0 6 2 281 2 0.0 6 2 283 2 0.0 6 2 284 1 0.0 6 1 285 1 0.0 6 1 286 1 0.0 6 1 288 1 0.0 6 1 289 1 0.0 6 1 291 1 0.0 6 1 297 2 0.0 6 2 298 1 0.0 6 1 299 1 0.0 6 1 301 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 58523 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 930.5 0 1 1 7 1 3.6 2 1 10 1 0.1 3 1 11 2 0.0 3 2 12 10 0.0 3 10 13 6 0.0 3 6 14 14 0.0 4 13 1 15 2 0.0 4 2 16 9 0.0 4 9 17 33 0.0 5 28 4 1 18 19 0.0 5 18 1 19 20 0.0 5 20 20 69 0.0 6 67 2 21 41 0.0 6 40 1 22 30 0.0 6 29 0 1 23 24 0.0 6 20 4 24 16 0.0 6 14 2 25 17 0.0 6 15 1 0 1 26 15 0.0 6 12 3 27 10 0.0 6 10 28 6 0.0 6 5 1 29 17 0.0 6 16 1 30 14 0.0 6 12 1 0 1 31 10 0.0 6 10 32 20 0.0 6 18 2 33 7 0.0 6 7 34 10 0.0 6 10 35 14 0.0 6 13 0 0 1 36 17 0.0 6 15 1 1 37 10 0.0 6 9 1 38 8 0.0 6 7 0 1 39 8 0.0 6 8 40 13 0.0 6 12 1 41 14 0.0 6 13 0 1 42 10 0.0 6 8 2 43 17 0.0 6 15 2 44 19 0.0 6 16 2 1 45 13 0.0 6 11 2 46 16 0.0 6 14 2 47 13 0.0 6 12 1 48 11 0.0 6 11 49 17 0.0 6 17 50 14 0.0 6 11 3 51 21 0.0 6 21 52 24 0.0 6 23 0 1 53 21 0.0 6 21 54 27 0.0 6 24 1 2 55 22 0.0 6 18 3 0 0 0 0 1 56 26 0.0 6 23 1 2 57 19 0.0 6 16 3 58 18 0.0 6 16 2 59 22 0.0 6 21 1 60 29 0.0 6 28 0 1 61 31 0.0 6 30 0 1 62 20 0.0 6 18 2 63 24 0.0 6 23 1 64 41 0.0 6 39 1 0 0 1 65 42 0.0 6 41 1 66 31 0.0 6 29 2 67 52 0.0 6 48 3 0 0 0 1 68 109 0.0 6 101 4 1 2 1 69 45 0.0 6 41 4 70 69 0.0 6 68 1 71 44 0.0 6 44 72 46 0.0 6 45 1 73 31 0.0 6 30 1 74 43 0.0 6 40 3 75 32 0.0 6 30 2 76 48 0.0 6 44 3 0 1 77 34 0.0 6 33 1 78 41 0.0 6 39 2 79 23 0.0 6 22 1 80 48 0.0 6 46 1 1 81 39 0.0 6 38 1 82 59 0.0 6 56 3 83 58 0.0 6 54 3 0 1 84 67 0.0 6 61 5 0 1 85 39 0.0 6 39 86 34 0.0 6 33 1 87 45 0.0 6 43 2 88 43 0.0 6 42 1 89 62 0.0 6 58 4 90 41 0.0 6 40 1 91 63 0.0 6 61 1 0 0 0 1 92 71 0.0 6 66 4 1 93 70 0.0 6 64 4 0 0 1 1 94 95 0.0 6 94 1 95 82 0.0 6 80 2 96 84 0.0 6 82 1 0 0 0 0 1 97 72 0.0 6 67 5 98 82 0.0 6 81 1 99 84 0.0 6 81 3 100 78 0.0 6 74 4 101 94 0.0 6 91 3 102 82 0.0 6 80 2 103 95 0.0 6 93 2 104 124 0.0 6 114 9 0 0 1 105 151 0.0 6 147 4 106 114 0.0 6 109 3 0 1 0 0 1 107 110 0.0 6 107 3 108 116 0.0 6 114 2 109 121 0.0 6 118 3 110 116 0.0 6 111 4 1 111 130 0.0 6 128 2 112 135 0.0 6 135 113 172 0.0 6 170 2 114 121 0.0 6 120 1 115 141 0.0 6 137 3 1 116 154 0.0 6 148 6 117 156 0.0 6 150 5 0 0 0 0 1 118 196 0.0 6 191 4 0 1 119 203 0.0 6 199 2 0 0 2 120 178 0.0 6 173 4 0 1 121 184 0.0 6 180 3 0 1 122 191 0.0 6 187 4 123 204 0.0 6 198 6 124 206 0.0 6 197 7 0 0 0 0 2 125 262 0.0 6 254 7 0 0 1 126 248 0.0 6 246 2 127 297 0.0 6 289 7 0 1 128 311 0.0 6 302 8 0 0 0 0 1 129 297 0.0 6 283 7 3 1 0 0 3 130 391 0.0 6 382 8 1 131 383 0.0 6 375 7 0 0 0 0 1 132 388 0.0 6 376 9 1 1 1 133 420 0.0 6 410 9 1 134 442 0.0 6 429 11 0 2 135 487 0.0 6 475 9 1 0 0 1 1 136 470 0.0 6 464 5 0 0 1 137 459 0.0 6 447 7 1 1 1 2 138 420 0.0 6 419 1 139 572 0.0 6 560 9 1 1 0 1 140 640 0.0 6 632 7 1 141 737 0.0 6 728 7 0 0 0 2 142 752 0.0 6 737 12 1 0 2 143 767 0.0 6 756 11 144 801 0.0 6 791 8 1 1 145 836 0.0 6 823 11 0 0 1 0 1 146 851 0.0 6 835 14 0 0 1 1 147 888 0.0 6 873 14 1 148 948 0.0 6 935 9 0 2 1 0 1 149 1010 0.0 6 985 21 1 2 0 1 150 1138 0.0 6 1117 12 0 2 1 3 3 151 1369 0.0 6 1344 20 3 2 152 1057 0.0 6 1038 14 4 0 0 0 1 153 1304 0.0 6 1276 22 2 2 0 1 1 154 1277 0.0 6 1254 18 0 1 1 1 2 155 1078 0.0 6 1055 20 0 1 1 1 156 1188 0.0 6 1173 11 1 1 1 0 1 157 1017 0.0 6 996 19 0 2 158 1113 0.0 6 1097 12 3 0 0 0 1 159 1109 0.0 6 1093 13 3 160 1058 0.0 6 1041 14 1 1 1 161 936 0.0 6 931 2 0 1 1 1 162 861 0.0 6 849 6 0 4 2 163 786 0.0 6 776 9 0 0 1 164 827 0.0 6 817 10 165 770 0.0 6 762 5 0 1 1 1 166 760 0.0 6 752 5 2 0 0 1 167 904 0.0 6 901 3 168 739 0.0 6 733 3 1 1 0 0 1 169 925 0.0 6 914 10 1 170 756 0.0 6 747 7 0 2 171 798 0.0 6 788 7 1 1 1 172 883 0.0 6 874 7 0 0 0 1 1 173 815 0.0 6 804 11 174 796 0.0 6 788 7 0 1 175 800 0.0 6 795 5 176 779 0.0 6 769 9 0 0 1 177 820 0.0 6 810 9 0 0 0 0 1 178 583 0.0 6 571 9 0 0 0 2 1 179 613 0.0 6 608 4 0 0 0 0 1 180 493 0.0 6 487 5 1 181 464 0.0 6 460 2 0 0 1 1 182 400 0.0 6 396 4 183 487 0.0 6 477 9 1 184 674 0.0 6 667 7 185 708 0.0 6 702 6 186 618 0.0 6 612 6 187 425 0.0 6 422 3 188 385 0.0 6 381 4 189 428 0.0 6 425 2 0 1 190 407 0.0 6 402 4 1 191 312 0.0 6 311 1 192 334 0.0 6 328 5 1 193 267 0.0 6 264 2 1 194 268 0.0 6 266 1 1 195 258 0.0 6 257 1 196 216 0.0 6 214 2 197 291 0.0 6 289 1 0 1 198 239 0.0 6 235 3 1 199 195 0.0 6 192 3 200 173 0.0 6 172 0 0 0 1 201 164 0.0 6 161 2 0 1 202 139 0.0 6 139 203 163 0.0 6 162 1 204 131 0.0 6 130 1 205 107 0.0 6 106 0 0 1 206 101 0.0 6 101 207 112 0.0 6 111 1 208 105 0.0 6 105 209 101 0.0 6 101 210 82 0.0 6 81 1 211 54 0.0 6 54 212 65 0.0 6 65 213 65 0.0 6 64 1 214 66 0.0 6 65 1 215 66 0.0 6 64 1 0 1 216 49 0.0 6 48 1 217 48 0.0 6 48 218 29 0.0 6 28 0 1 219 33 0.0 6 33 220 24 0.0 6 24 221 31 0.0 6 31 222 43 0.0 6 42 1 223 24 0.0 6 24 224 14 0.0 6 14 225 19 0.0 6 18 1 226 18 0.0 6 18 227 18 0.0 6 18 228 15 0.0 6 15 229 8 0.0 6 8 230 7 0.0 6 7 231 2 0.0 6 2 232 7 0.0 6 7 233 6 0.0 6 6 234 2 0.0 6 2 235 2 0.0 6 2 236 4 0.0 6 4 237 4 0.0 6 4 238 5 0.0 6 5 239 3 0.0 6 3 240 3 0.0 6 3 242 7 0.0 6 7 243 4 0.0 6 4 244 1 0.0 6 1 246 1 0.0 6 1 249 2 0.0 6 2 252 1 0.0 6 1 253 1 0.0 6 1 279 11 0.0 6 11 280 94 0.0 6 90 3 0 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 960 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 2 0.0 6 2 71 7 0.0 6 7 72 8 0.0 6 8 73 11 0.0 6 11 74 3 0.0 6 3 75 4 0.0 6 4 76 11 0.0 6 11 77 12 0.0 6 12 78 8 0.0 6 8 79 14 0.0 6 14 80 10 0.0 6 10 81 5 0.0 6 5 82 4 0.0 6 4 83 4 0.0 6 4 84 6 0.0 6 6 85 4 0.0 6 4 86 8 0.0 6 8 87 4 0.0 6 4 88 8 0.0 6 8 89 6 0.0 6 6 90 5 0.0 6 5 91 9 0.0 6 9 92 6 0.0 6 6 93 7 0.0 6 7 94 9 0.0 6 9 95 6 0.0 6 6 96 3 0.0 6 3 97 6 0.0 6 6 98 9 0.0 6 9 99 6 0.0 6 6 100 9 0.0 6 9 101 10 0.0 6 10 102 5 0.0 6 5 103 13 0.0 6 13 104 11 0.0 6 11 105 8 0.0 6 8 106 10 0.0 6 9 0 0 1 107 4 0.0 6 4 108 11 0.0 6 11 109 27 0.0 6 27 110 5 0.0 6 5 111 8 0.0 6 8 112 6 0.0 6 5 0 0 0 0 0 1 113 4 0.0 6 4 114 6 0.0 6 6 115 8 0.0 6 8 116 4 0.0 6 4 117 6 0.0 6 6 118 1 0.0 6 1 119 6 0.0 6 6 120 5 0.0 6 5 121 12 0.0 6 11 0 0 0 1 122 6 0.0 6 6 123 8 0.0 6 6 1 0 0 1 124 16 0.0 6 16 126 4 0.0 6 4 127 8 0.0 6 8 128 5 0.0 6 5 129 2 0.0 6 2 130 8 0.0 6 8 131 5 0.0 6 5 132 10 0.0 6 10 133 13 0.0 6 13 134 2 0.0 6 2 135 5 0.0 6 5 136 16 0.0 6 16 137 9 0.0 6 9 138 9 0.0 6 9 139 12 0.0 6 11 1 140 4 0.0 6 4 141 7 0.0 6 7 142 9 0.0 6 9 143 8 0.0 6 8 144 6 0.0 6 6 145 6 0.0 6 6 146 5 0.0 6 5 147 6 0.0 6 6 148 9 0.0 6 9 149 8 0.0 6 8 150 5 0.0 6 5 151 8 0.0 6 8 152 2 0.0 6 2 153 7 0.0 6 7 154 9 0.0 6 9 155 8 0.0 6 8 156 3 0.0 6 3 157 13 0.0 6 13 158 8 0.0 6 8 159 6 0.0 6 6 160 6 0.0 6 6 161 10 0.0 6 10 162 10 0.0 6 10 163 5 0.0 6 5 164 6 0.0 6 6 165 6 0.0 6 5 0 0 0 1 166 13 0.0 6 13 167 12 0.0 6 12 168 7 0.0 6 7 169 14 0.0 6 14 170 10 0.0 6 10 171 6 0.0 6 6 172 9 0.0 6 8 0 0 0 1 173 4 0.0 6 4 174 7 0.0 6 7 175 4 0.0 6 4 176 8 0.0 6 8 177 5 0.0 6 5 178 7 0.0 6 7 179 7 0.0 6 7 180 8 0.0 6 8 181 6 0.0 6 6 182 2 0.0 6 2 183 2 0.0 6 2 184 11 0.0 6 11 185 4 0.0 6 3 0 0 0 0 0 1 186 2 0.0 6 2 187 4 0.0 6 4 188 8 0.0 6 7 0 0 1 189 8 0.0 6 8 190 6 0.0 6 6 191 4 0.0 6 4 192 7 0.0 6 7 193 3 0.0 6 3 194 5 0.0 6 5 195 4 0.0 6 4 196 3 0.0 6 3 197 5 0.0 6 5 199 2 0.0 6 2 200 4 0.0 6 4 201 2 0.0 6 2 202 4 0.0 6 4 204 2 0.0 6 2 205 1 0.0 6 1 206 2 0.0 6 2 207 5 0.0 6 5 208 4 0.0 6 4 209 3 0.0 6 3 210 1 0.0 6 1 211 2 0.0 6 2 212 4 0.0 6 4 213 2 0.0 6 2 215 1 0.0 6 1 218 2 0.0 6 2 219 1 0.0 6 1 220 1 0.0 6 1 222 2 0.0 6 2 223 1 0.0 6 1 227 1 0.0 6 1 230 3 0.0 6 3 246 1 0.0 6 1 250 1 0.0 6 1 255 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_13_212_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_13_439_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_13_212_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_13_439_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.25 s (127257 µs/read; 0.00 M reads/minute). === Summary === Total read pairs processed: 2 Read 1 with adapter: 1 (50.0%) Read 2 with adapter: 1 (50.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 1 (50.0%) Pairs written (passing filters): 1 (50.0%) Total basepairs processed: 682 bp Read 1: 418 bp Read 2: 264 bp Total written (filtered): 63 bp (9.2%) Read 1: 31 bp Read 2: 32 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 1 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 137 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 0 times === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 1 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 23 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 0 times This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_07_213_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_07_440_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_07_213_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_07_440_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.38 s (37 µs/read; 1.63 M reads/minute). === Summary === Total read pairs processed: 91,994 Read 1 with adapter: 91,994 (100.0%) Read 2 with adapter: 91,911 (99.9%) == Read fate breakdown == Pairs that were too short: 40 (0.0%) Pairs discarded as untrimmed: 83 (0.1%) Pairs written (passing filters): 91,871 (99.9%) Total basepairs processed: 40,972,537 bp Read 1: 23,016,371 bp Read 2: 17,956,166 bp Total written (filtered): 5,979,553 bp (14.6%) Read 1: 3,006,784 bp Read 2: 2,972,769 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 90583 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 5 1437.4 0 1 4 4 4 359.4 1 3 1 6 4 22.5 1 0 0 4 7 1 5.6 2 0 0 1 9 1 0.4 2 1 12 1 0.0 3 0 1 15 2 0.0 4 2 16 2 0.0 4 1 1 17 3 0.0 5 3 19 5 0.0 5 5 20 100 0.0 6 97 3 21 44 0.0 6 43 1 22 54 0.0 6 54 23 37 0.0 6 36 1 24 31 0.0 6 31 25 30 0.0 6 30 26 22 0.0 6 20 2 27 11 0.0 6 11 28 14 0.0 6 12 1 0 1 29 7 0.0 6 7 30 7 0.0 6 6 1 31 9 0.0 6 8 1 32 8 0.0 6 8 33 6 0.0 6 6 34 4 0.0 6 3 1 35 2 0.0 6 2 36 5 0.0 6 5 37 4 0.0 6 3 1 38 5 0.0 6 5 39 14 0.0 6 14 40 11 0.0 6 10 1 41 9 0.0 6 9 42 13 0.0 6 12 1 43 9 0.0 6 9 44 12 0.0 6 11 1 45 6 0.0 6 6 46 7 0.0 6 7 47 10 0.0 6 8 0 2 48 8 0.0 6 8 49 7 0.0 6 7 50 8 0.0 6 6 2 51 7 0.0 6 6 1 52 3 0.0 6 3 53 4 0.0 6 4 54 13 0.0 6 12 1 55 5 0.0 6 5 56 7 0.0 6 5 0 0 0 0 2 57 4 0.0 6 4 58 9 0.0 6 8 1 59 5 0.0 6 5 60 4 0.0 6 4 61 5 0.0 6 5 62 4 0.0 6 4 63 10 0.0 6 10 64 8 0.0 6 8 65 8 0.0 6 8 66 7 0.0 6 7 67 11 0.0 6 11 68 9 0.0 6 9 69 5 0.0 6 5 70 6 0.0 6 5 0 0 0 1 71 18 0.0 6 16 2 72 14 0.0 6 14 73 12 0.0 6 12 74 11 0.0 6 11 75 12 0.0 6 12 76 9 0.0 6 9 77 10 0.0 6 10 78 14 0.0 6 14 79 7 0.0 6 6 0 0 0 0 0 1 80 6 0.0 6 6 81 6 0.0 6 6 82 13 0.0 6 13 83 21 0.0 6 20 0 0 1 84 16 0.0 6 16 85 6 0.0 6 6 86 9 0.0 6 9 87 17 0.0 6 15 1 0 0 0 0 1 88 11 0.0 6 11 89 14 0.0 6 13 1 90 12 0.0 6 11 1 91 8 0.0 6 8 92 12 0.0 6 12 93 7 0.0 6 7 94 10 0.0 6 10 95 7 0.0 6 7 96 11 0.0 6 10 1 97 16 0.0 6 14 1 1 98 17 0.0 6 14 1 1 0 0 1 99 9 0.0 6 9 100 17 0.0 6 15 2 101 28 0.0 6 27 1 102 16 0.0 6 14 2 103 25 0.0 6 25 104 20 0.0 6 19 0 0 0 0 0 1 105 20 0.0 6 17 2 0 0 0 0 1 106 18 0.0 6 17 1 107 21 0.0 6 20 1 108 21 0.0 6 19 1 1 109 14 0.0 6 13 1 110 18 0.0 6 18 111 15 0.0 6 15 112 25 0.0 6 24 1 113 24 0.0 6 22 1 0 0 0 0 1 114 29 0.0 6 29 115 31 0.0 6 31 116 21 0.0 6 18 2 0 1 117 32 0.0 6 31 0 1 118 26 0.0 6 25 1 119 28 0.0 6 27 1 120 28 0.0 6 27 1 121 15 0.0 6 14 1 122 35 0.0 6 34 1 123 37 0.0 6 36 1 124 27 0.0 6 27 125 36 0.0 6 34 2 126 23 0.0 6 23 127 26 0.0 6 24 1 1 128 33 0.0 6 32 1 129 44 0.0 6 42 2 130 45 0.0 6 45 131 38 0.0 6 38 132 34 0.0 6 34 133 49 0.0 6 48 1 134 48 0.0 6 47 1 135 62 0.0 6 60 2 136 45 0.0 6 43 1 1 137 57 0.0 6 55 2 138 61 0.0 6 60 1 139 65 0.0 6 64 1 140 71 0.0 6 71 141 58 0.0 6 57 1 142 107 0.0 6 107 143 161 0.0 6 160 1 144 361 0.0 6 360 1 145 464 0.0 6 463 1 146 483 0.0 6 482 1 147 344 0.0 6 344 148 541 0.0 6 541 149 427 0.0 6 426 1 150 492 0.0 6 490 2 151 453 0.0 6 453 152 451 0.0 6 450 1 153 584 0.0 6 584 154 430 0.0 6 428 2 155 390 0.0 6 390 156 352 0.0 6 351 0 0 1 157 343 0.0 6 341 2 158 309 0.0 6 309 159 312 0.0 6 310 2 160 341 0.0 6 338 3 161 309 0.0 6 308 1 162 355 0.0 6 354 1 163 397 0.0 6 396 1 164 437 0.0 6 436 1 165 400 0.0 6 400 166 428 0.0 6 425 2 0 0 1 167 397 0.0 6 396 1 168 277 0.0 6 273 4 169 371 0.0 6 366 4 0 0 1 170 349 0.0 6 349 171 347 0.0 6 346 1 172 447 0.0 6 444 3 173 333 0.0 6 332 1 174 371 0.0 6 364 7 175 428 0.0 6 427 1 176 358 0.0 6 357 1 177 324 0.0 6 322 2 178 322 0.0 6 321 1 179 382 0.0 6 379 2 0 1 180 357 0.0 6 354 3 181 910 0.0 6 904 4 0 0 0 0 2 182 491 0.0 6 489 2 183 469 0.0 6 466 3 184 412 0.0 6 411 1 185 447 0.0 6 442 5 186 608 0.0 6 605 3 187 528 0.0 6 526 1 0 0 0 1 188 428 0.0 6 423 4 1 189 426 0.0 6 424 1 0 1 190 448 0.0 6 444 3 0 0 1 191 445 0.0 6 443 2 192 477 0.0 6 473 4 193 570 0.0 6 564 5 0 0 0 1 194 557 0.0 6 553 3 0 0 0 0 1 195 833 0.0 6 822 10 0 0 0 0 1 196 896 0.0 6 888 8 197 830 0.0 6 825 5 198 982 0.0 6 972 9 0 0 1 199 860 0.0 6 850 10 200 693 0.0 6 689 4 201 717 0.0 6 714 2 1 202 701 0.0 6 695 6 203 718 0.0 6 709 7 1 0 1 204 755 0.0 6 749 6 205 1029 0.0 6 1018 9 0 0 0 1 1 206 982 0.0 6 978 4 207 787 0.0 6 781 5 1 208 838 0.0 6 830 8 209 645 0.0 6 641 4 210 690 0.0 6 687 2 0 0 0 0 1 211 812 0.0 6 808 4 212 877 0.0 6 866 9 0 0 0 2 213 908 0.0 6 903 5 214 1065 0.0 6 1058 7 215 1091 0.0 6 1075 14 1 1 216 900 0.0 6 891 9 217 865 0.0 6 858 6 0 0 1 218 941 0.0 6 933 7 1 219 911 0.0 6 904 7 220 652 0.0 6 649 3 221 628 0.0 6 625 3 222 801 0.0 6 789 12 223 933 0.0 6 927 6 224 830 0.0 6 824 6 225 926 0.0 6 915 10 0 0 1 226 872 0.0 6 867 5 227 707 0.0 6 702 5 228 638 0.0 6 635 3 229 689 0.0 6 684 4 0 1 230 647 0.0 6 642 5 231 667 0.0 6 664 3 232 617 0.0 6 613 4 233 611 0.0 6 607 3 1 234 579 0.0 6 572 7 235 485 0.0 6 481 4 236 548 0.0 6 540 8 237 685 0.0 6 673 10 1 0 0 0 1 238 813 0.0 6 804 8 1 239 714 0.0 6 709 5 240 693 0.0 6 682 11 241 693 0.0 6 683 9 0 1 242 685 0.0 6 673 12 243 554 0.0 6 552 2 244 551 0.0 6 544 6 1 245 518 0.0 6 507 10 0 1 246 487 0.0 6 478 8 1 247 393 0.0 6 387 6 248 469 0.0 6 464 5 249 492 0.0 6 482 10 250 479 0.0 6 469 10 251 573 0.0 6 565 7 1 252 363 0.0 6 361 2 253 354 0.0 6 351 3 254 356 0.0 6 351 5 255 419 0.0 6 412 6 1 256 500 0.0 6 494 6 257 557 0.0 6 552 4 0 0 0 1 258 379 0.0 6 376 3 259 618 0.0 6 603 15 260 719 0.0 6 712 7 261 287 0.0 6 279 8 262 272 0.0 6 271 1 263 249 0.0 6 246 3 264 276 0.0 6 272 2 1 0 0 1 265 291 0.0 6 286 5 266 443 0.0 6 433 9 1 267 298 0.0 6 296 2 268 477 0.0 6 464 12 1 269 771 0.0 6 758 12 1 270 395 0.0 6 386 9 271 374 0.0 6 369 4 1 272 318 0.0 6 309 8 1 273 716 0.0 6 700 16 274 1154 0.0 6 1125 28 1 275 561 0.0 6 547 14 276 694 0.0 6 667 25 2 277 1042 0.0 6 1010 28 4 278 9568 0.0 6 9313 249 5 0 1 279 1787 0.0 6 1723 62 2 280 35 0.0 6 30 2 1 2 281 4 0.0 6 1 2 1 282 1 0.0 6 1 283 1 0.0 6 1 284 1 0.0 6 1 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1411 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 14 359.4 1 0 14 57 1 0.0 6 1 59 1 0.0 6 1 61 1 0.0 6 1 63 55 0.0 6 54 1 64 1 0.0 6 1 67 1 0.0 6 1 71 2 0.0 6 2 72 3 0.0 6 3 73 12 0.0 6 12 74 7 0.0 6 7 75 13 0.0 6 12 1 76 15 0.0 6 15 77 22 0.0 6 22 78 8 0.0 6 8 79 15 0.0 6 15 80 15 0.0 6 15 81 14 0.0 6 14 82 13 0.0 6 13 83 10 0.0 6 10 84 6 0.0 6 6 85 8 0.0 6 8 86 12 0.0 6 12 87 6 0.0 6 6 88 10 0.0 6 10 89 10 0.0 6 10 90 3 0.0 6 3 91 6 0.0 6 6 92 6 0.0 6 6 93 15 0.0 6 15 94 10 0.0 6 10 95 8 0.0 6 8 96 5 0.0 6 5 97 9 0.0 6 9 98 8 0.0 6 8 99 10 0.0 6 10 100 11 0.0 6 11 101 8 0.0 6 8 102 4 0.0 6 4 103 4 0.0 6 4 104 6 0.0 6 6 105 6 0.0 6 6 106 3 0.0 6 3 107 8 0.0 6 8 108 9 0.0 6 9 109 14 0.0 6 14 110 13 0.0 6 13 111 9 0.0 6 9 112 7 0.0 6 7 113 9 0.0 6 9 114 7 0.0 6 7 115 10 0.0 6 10 116 7 0.0 6 7 117 6 0.0 6 6 118 2 0.0 6 2 119 7 0.0 6 7 120 4 0.0 6 4 121 6 0.0 6 6 122 6 0.0 6 6 123 6 0.0 6 6 124 7 0.0 6 7 125 13 0.0 6 13 126 2 0.0 6 2 127 8 0.0 6 8 128 12 0.0 6 12 129 4 0.0 6 4 130 6 0.0 6 6 131 4 0.0 6 4 132 10 0.0 6 10 133 10 0.0 6 10 134 13 0.0 6 13 135 1 0.0 6 1 136 6 0.0 6 6 137 6 0.0 6 6 138 4 0.0 6 4 139 16 0.0 6 16 140 5 0.0 6 5 141 4 0.0 6 4 142 7 0.0 6 7 143 7 0.0 6 7 144 7 0.0 6 7 145 6 0.0 6 6 146 11 0.0 6 11 147 5 0.0 6 5 148 8 0.0 6 7 1 149 4 0.0 6 4 150 7 0.0 6 7 151 13 0.0 6 13 152 6 0.0 6 6 153 5 0.0 6 5 154 8 0.0 6 8 155 7 0.0 6 7 156 10 0.0 6 10 157 12 0.0 6 12 158 7 0.0 6 7 159 5 0.0 6 4 1 160 6 0.0 6 6 161 5 0.0 6 5 162 12 0.0 6 12 163 13 0.0 6 13 164 16 0.0 6 16 165 13 0.0 6 13 166 14 0.0 6 14 167 6 0.0 6 6 168 7 0.0 6 7 169 6 0.0 6 6 170 14 0.0 6 14 171 5 0.0 6 5 172 13 0.0 6 13 173 3 0.0 6 3 174 5 0.0 6 5 175 13 0.0 6 13 176 5 0.0 6 5 177 7 0.0 6 7 178 6 0.0 6 6 179 8 0.0 6 8 180 5 0.0 6 5 181 6 0.0 6 6 182 6 0.0 6 6 183 4 0.0 6 4 184 12 0.0 6 12 185 3 0.0 6 3 186 11 0.0 6 11 187 13 0.0 6 13 188 9 0.0 6 9 189 7 0.0 6 7 190 14 0.0 6 14 191 11 0.0 6 11 192 9 0.0 6 9 193 10 0.0 6 10 194 5 0.0 6 5 195 4 0.0 6 4 196 9 0.0 6 9 197 4 0.0 6 4 198 5 0.0 6 5 199 4 0.0 6 4 200 10 0.0 6 10 201 4 0.0 6 4 202 11 0.0 6 11 203 15 0.0 6 15 204 5 0.0 6 5 205 7 0.0 6 7 206 3 0.0 6 3 207 5 0.0 6 5 208 5 0.0 6 5 209 5 0.0 6 5 210 9 0.0 6 9 211 8 0.0 6 8 212 12 0.0 6 12 213 5 0.0 6 5 214 4 0.0 6 4 215 6 0.0 6 6 216 2 0.0 6 2 217 3 0.0 6 3 218 11 0.0 6 10 0 0 0 0 0 1 219 13 0.0 6 13 220 3 0.0 6 3 221 6 0.0 6 6 222 2 0.0 6 2 223 8 0.0 6 7 1 224 5 0.0 6 5 225 7 0.0 6 7 226 5 0.0 6 5 227 4 0.0 6 4 228 5 0.0 6 5 229 7 0.0 6 7 230 6 0.0 6 6 231 3 0.0 6 3 232 2 0.0 6 2 233 5 0.0 6 5 234 3 0.0 6 3 235 2 0.0 6 2 236 3 0.0 6 3 237 4 0.0 6 4 238 4 0.0 6 4 239 5 0.0 6 5 240 2 0.0 6 2 241 2 0.0 6 2 242 5 0.0 6 5 243 1 0.0 6 1 244 1 0.0 6 1 245 1 0.0 6 1 246 1 0.0 6 1 247 4 0.0 6 4 248 3 0.0 6 3 249 2 0.0 6 2 250 2 0.0 6 2 251 6 0.0 6 6 253 3 0.0 6 3 254 1 0.0 6 1 255 1 0.0 6 1 256 2 0.0 6 2 257 1 0.0 6 1 258 3 0.0 6 3 260 1 0.0 6 1 261 1 0.0 6 1 262 1 0.0 6 1 263 5 0.0 6 5 264 3 0.0 6 3 265 1 0.0 6 1 267 1 0.0 6 1 274 1 0.0 6 1 279 1 0.0 6 1 281 1 0.0 6 1 307 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 90740 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 359.4 1 1 6 2 22.5 1 2 10 8 0.1 3 8 11 10 0.0 3 7 2 1 12 2 0.0 3 1 1 13 19 0.0 3 18 1 14 17 0.0 4 14 3 16 26 0.0 4 23 3 17 38 0.0 5 36 2 18 22 0.0 5 20 2 19 26 0.0 5 24 2 20 99 0.0 6 95 3 0 1 21 100 0.0 6 95 5 22 50 0.0 6 49 1 23 36 0.0 6 29 7 24 30 0.0 6 28 2 25 43 0.0 6 41 2 26 22 0.0 6 21 1 27 23 0.0 6 18 4 0 0 0 1 28 25 0.0 6 23 2 29 31 0.0 6 29 2 30 18 0.0 6 17 1 31 16 0.0 6 16 32 21 0.0 6 18 3 33 17 0.0 6 15 1 1 34 12 0.0 6 11 0 0 0 1 35 21 0.0 6 17 3 1 36 18 0.0 6 17 1 37 21 0.0 6 20 1 38 12 0.0 6 12 39 16 0.0 6 14 2 40 19 0.0 6 17 2 41 16 0.0 6 15 0 0 0 1 42 21 0.0 6 19 1 1 43 29 0.0 6 27 2 44 26 0.0 6 23 3 45 18 0.0 6 16 2 46 35 0.0 6 33 2 47 33 0.0 6 30 2 1 48 23 0.0 6 21 2 49 38 0.0 6 36 2 50 15 0.0 6 15 51 32 0.0 6 31 0 1 52 33 0.0 6 29 4 53 33 0.0 6 31 1 1 54 24 0.0 6 19 5 55 34 0.0 6 31 2 1 56 28 0.0 6 26 2 57 38 0.0 6 33 5 58 35 0.0 6 32 2 1 59 34 0.0 6 33 1 60 33 0.0 6 32 0 1 61 25 0.0 6 24 0 0 1 62 38 0.0 6 35 3 63 41 0.0 6 40 1 64 31 0.0 6 29 1 0 0 1 65 29 0.0 6 25 4 66 50 0.0 6 46 3 1 67 181 0.0 6 177 4 68 58 0.0 6 53 4 0 0 1 69 83 0.0 6 82 1 70 52 0.0 6 50 1 0 0 0 1 71 60 0.0 6 56 3 0 0 1 72 42 0.0 6 41 0 1 73 35 0.0 6 35 74 60 0.0 6 53 7 75 44 0.0 6 39 4 1 76 62 0.0 6 57 4 0 0 0 1 77 63 0.0 6 61 2 78 55 0.0 6 50 3 1 0 1 79 80 0.0 6 74 4 0 2 80 64 0.0 6 58 5 1 81 60 0.0 6 58 2 82 67 0.0 6 62 4 1 83 108 0.0 6 103 4 0 0 0 1 84 70 0.0 6 68 1 1 85 53 0.0 6 49 4 86 61 0.0 6 58 3 87 78 0.0 6 72 5 1 88 83 0.0 6 77 6 89 65 0.0 6 60 5 90 76 0.0 6 71 4 1 91 79 0.0 6 72 7 92 78 0.0 6 74 4 93 102 0.0 6 99 3 94 120 0.0 6 110 9 1 95 97 0.0 6 94 3 96 128 0.0 6 120 6 0 1 1 97 105 0.0 6 101 4 98 100 0.0 6 94 6 99 97 0.0 6 95 2 100 109 0.0 6 107 1 1 101 124 0.0 6 118 6 102 125 0.0 6 122 3 103 152 0.0 6 146 6 104 204 0.0 6 192 9 1 0 2 105 123 0.0 6 118 5 106 107 0.0 6 102 5 107 141 0.0 6 135 6 108 130 0.0 6 126 4 109 128 0.0 6 124 4 110 162 0.0 6 159 3 111 161 0.0 6 152 5 2 0 2 112 182 0.0 6 179 1 1 0 1 113 181 0.0 6 171 8 0 0 0 0 2 114 169 0.0 6 161 6 1 0 1 115 198 0.0 6 191 7 116 188 0.0 6 182 4 1 1 117 210 0.0 6 208 2 118 231 0.0 6 228 3 119 207 0.0 6 198 8 0 0 0 0 1 120 223 0.0 6 216 6 1 121 231 0.0 6 224 6 1 122 222 0.0 6 215 6 1 123 256 0.0 6 248 8 124 307 0.0 6 300 7 125 311 0.0 6 297 12 1 0 0 0 1 126 313 0.0 6 301 10 1 0 0 0 1 127 375 0.0 6 359 14 0 1 1 128 353 0.0 6 348 5 129 341 0.0 6 331 10 130 401 0.0 6 394 5 0 1 0 0 1 131 479 0.0 6 464 15 132 477 0.0 6 469 8 133 539 0.0 6 528 8 1 0 1 1 134 580 0.0 6 565 11 1 0 1 1 1 135 595 0.0 6 582 12 0 0 1 136 497 0.0 6 487 9 0 0 1 137 487 0.0 6 478 6 2 0 0 0 1 138 503 0.0 6 492 9 2 139 605 0.0 6 599 3 3 140 737 0.0 6 717 20 141 947 0.0 6 929 14 0 1 1 0 2 142 1124 0.0 6 1113 9 0 0 1 1 143 1048 0.0 6 1029 15 2 1 0 1 144 1082 0.0 6 1062 19 0 0 1 145 1152 0.0 6 1140 9 0 0 2 0 1 146 1149 0.0 6 1134 12 0 1 1 0 1 147 1148 0.0 6 1140 8 148 1383 0.0 6 1363 16 2 0 0 0 2 149 1479 0.0 6 1450 23 4 1 0 0 1 150 1962 0.0 6 1942 18 1 0 0 1 151 1363 0.0 6 1338 24 0 1 152 1682 0.0 6 1648 26 3 0 2 0 3 153 1531 0.0 6 1513 18 154 1205 0.0 6 1185 16 1 1 1 1 155 1255 0.0 6 1239 14 1 0 0 0 1 156 1153 0.0 6 1130 21 2 157 1313 0.0 6 1295 12 1 3 1 0 1 158 1351 0.0 6 1329 17 0 2 1 2 159 1131 0.0 6 1116 12 1 1 0 1 160 1208 0.0 6 1193 13 0 0 1 1 161 1092 0.0 6 1077 15 162 953 0.0 6 935 15 2 0 0 1 163 1021 0.0 6 1011 10 164 1051 0.0 6 1039 12 165 977 0.0 6 965 12 166 1201 0.0 6 1189 11 0 1 167 961 0.0 6 944 14 2 1 168 1168 0.0 6 1157 9 0 1 0 1 169 879 0.0 6 863 12 1 2 0 1 170 889 0.0 6 882 6 1 171 1065 0.0 6 1059 6 172 1034 0.0 6 1022 9 1 1 0 0 1 173 1116 0.0 6 1102 14 174 1083 0.0 6 1064 19 175 1275 0.0 6 1250 21 3 0 1 176 1202 0.0 6 1188 13 0 1 177 920 0.0 6 905 13 0 1 0 1 178 959 0.0 6 952 6 0 0 1 179 773 0.0 6 761 9 0 1 0 0 2 180 708 0.0 6 692 12 2 1 0 1 181 738 0.0 6 726 11 1 182 506 0.0 6 498 8 183 863 0.0 6 850 11 0 0 1 0 1 184 957 0.0 6 946 11 185 1048 0.0 6 1023 24 0 0 1 186 1138 0.0 6 1118 18 1 0 0 0 1 187 988 0.0 6 967 21 188 971 0.0 6 950 17 1 2 1 189 827 0.0 6 812 14 1 190 1113 0.0 6 1083 28 1 0 1 191 1114 0.0 6 1094 18 0 1 1 192 694 0.0 6 682 11 1 193 532 0.0 6 518 14 194 730 0.0 6 712 17 0 0 1 195 966 0.0 6 941 25 196 1387 0.0 6 1353 32 0 0 2 197 715 0.0 6 692 21 1 0 1 198 1246 0.0 6 1202 42 2 199 1302 0.0 6 1273 25 3 0 1 200 947 0.0 6 925 22 201 829 0.0 6 809 19 1 202 489 0.0 6 476 13 203 484 0.0 6 472 11 0 0 1 204 466 0.0 6 457 9 205 253 0.0 6 251 2 206 241 0.0 6 237 4 207 224 0.0 6 217 7 208 258 0.0 6 252 6 209 202 0.0 6 196 6 210 192 0.0 6 186 6 211 179 0.0 6 173 6 212 194 0.0 6 190 4 213 154 0.0 6 150 3 1 214 381 0.0 6 360 20 1 215 320 0.0 6 307 12 1 216 306 0.0 6 296 8 1 0 1 217 272 0.0 6 264 7 1 218 233 0.0 6 229 4 219 253 0.0 6 240 11 1 0 1 220 144 0.0 6 140 4 221 303 0.0 6 290 11 1 0 1 222 227 0.0 6 222 5 223 438 0.0 6 425 12 1 224 245 0.0 6 237 7 1 225 269 0.0 6 265 4 226 201 0.0 6 197 4 227 181 0.0 6 176 5 228 155 0.0 6 151 3 1 229 181 0.0 6 177 3 0 1 230 117 0.0 6 112 5 231 193 0.0 6 187 6 232 209 0.0 6 204 5 233 116 0.0 6 114 2 234 95 0.0 6 91 4 235 42 0.0 6 42 236 8 0.0 6 8 237 18 0.0 6 16 2 238 18 0.0 6 18 239 31 0.0 6 29 2 240 50 0.0 6 47 3 241 106 0.0 6 99 7 242 77 0.0 6 74 3 243 51 0.0 6 50 1 244 58 0.0 6 57 1 245 39 0.0 6 39 246 36 0.0 6 36 247 17 0.0 6 17 248 52 0.0 6 50 1 1 249 10 0.0 6 9 1 250 10 0.0 6 10 251 12 0.0 6 11 1 252 2 0.0 6 2 254 2 0.0 6 2 273 1 0.0 6 1 277 1 0.0 6 1 278 12 0.0 6 12 279 65 0.0 6 63 2 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1171 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 49 1 0.0 6 0 1 54 1 0.0 6 1 60 1 0.0 6 1 71 1 0.0 6 1 72 3 0.0 6 3 73 10 0.0 6 10 74 16 0.0 6 16 75 12 0.0 6 12 76 9 0.0 6 9 77 14 0.0 6 14 78 12 0.0 6 12 79 16 0.0 6 16 80 14 0.0 6 14 81 20 0.0 6 20 82 19 0.0 6 19 83 6 0.0 6 6 84 9 0.0 6 9 85 9 0.0 6 9 86 9 0.0 6 9 87 12 0.0 6 12 88 7 0.0 6 7 89 6 0.0 6 6 90 11 0.0 6 11 91 14 0.0 6 14 92 9 0.0 6 9 93 15 0.0 6 15 94 7 0.0 6 7 95 7 0.0 6 7 96 9 0.0 6 9 97 7 0.0 6 7 98 9 0.0 6 9 99 10 0.0 6 10 100 9 0.0 6 9 101 15 0.0 6 15 102 10 0.0 6 9 0 0 0 1 103 7 0.0 6 7 104 15 0.0 6 15 105 7 0.0 6 7 106 9 0.0 6 9 107 13 0.0 6 13 108 6 0.0 6 6 109 8 0.0 6 7 0 0 1 110 26 0.0 6 26 111 18 0.0 6 18 112 8 0.0 6 8 113 12 0.0 6 12 114 10 0.0 6 10 115 12 0.0 6 11 1 116 8 0.0 6 8 117 7 0.0 6 7 118 9 0.0 6 9 119 3 0.0 6 3 120 10 0.0 6 10 121 7 0.0 6 6 0 0 0 1 122 3 0.0 6 3 123 4 0.0 6 4 124 11 0.0 6 11 125 9 0.0 6 9 126 8 0.0 6 8 127 14 0.0 6 14 128 19 0.0 6 19 129 10 0.0 6 10 130 5 0.0 6 5 131 7 0.0 6 7 132 4 0.0 6 4 133 7 0.0 6 7 134 22 0.0 6 22 135 5 0.0 6 5 136 6 0.0 6 6 137 10 0.0 6 10 138 6 0.0 6 5 0 0 0 1 139 4 0.0 6 4 140 6 0.0 6 6 141 12 0.0 6 12 142 12 0.0 6 12 143 5 0.0 6 5 144 4 0.0 6 4 145 11 0.0 6 11 146 6 0.0 6 6 147 9 0.0 6 9 148 6 0.0 6 6 149 11 0.0 6 11 150 13 0.0 6 13 151 8 0.0 6 8 152 8 0.0 6 8 153 5 0.0 6 4 0 0 0 1 154 2 0.0 6 2 155 5 0.0 6 5 156 4 0.0 6 4 157 10 0.0 6 10 158 17 0.0 6 17 159 7 0.0 6 7 160 7 0.0 6 7 161 10 0.0 6 10 162 11 0.0 6 11 163 17 0.0 6 17 164 21 0.0 6 21 165 10 0.0 6 10 166 6 0.0 6 6 167 8 0.0 6 8 168 7 0.0 6 7 169 8 0.0 6 8 170 10 0.0 6 9 0 0 0 0 0 1 171 9 0.0 6 9 172 6 0.0 6 6 173 9 0.0 6 9 174 8 0.0 6 8 175 3 0.0 6 3 176 8 0.0 6 8 177 11 0.0 6 11 178 5 0.0 6 5 179 7 0.0 6 7 180 14 0.0 6 14 181 10 0.0 6 10 182 9 0.0 6 9 183 1 0.0 6 1 184 11 0.0 6 10 0 1 185 3 0.0 6 3 186 6 0.0 6 6 187 9 0.0 6 9 188 7 0.0 6 7 189 3 0.0 6 3 190 2 0.0 6 2 191 9 0.0 6 9 192 2 0.0 6 2 193 3 0.0 6 3 194 3 0.0 6 3 195 2 0.0 6 2 196 2 0.0 6 2 197 3 0.0 6 3 198 3 0.0 6 3 199 4 0.0 6 4 200 4 0.0 6 4 201 3 0.0 6 3 202 1 0.0 6 1 203 5 0.0 6 5 204 1 0.0 6 1 205 2 0.0 6 2 206 4 0.0 6 4 208 2 0.0 6 2 209 1 0.0 6 1 210 1 0.0 6 1 211 1 0.0 6 1 212 3 0.0 6 3 213 1 0.0 6 1 215 1 0.0 6 1 217 1 0.0 6 1 219 1 0.0 6 1 233 2 0.0 6 2 238 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_08_214_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_08_441_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_08_214_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_08_441_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.54 s (35 µs/read; 1.69 M reads/minute). === Summary === Total read pairs processed: 71,697 Read 1 with adapter: 71,697 (100.0%) Read 2 with adapter: 71,697 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 0 (0.0%) Pairs written (passing filters): 71,697 (100.0%) Total basepairs processed: 36,255,288 bp Read 1: 20,459,259 bp Read 2: 15,796,029 bp Total written (filtered): 4,518,833 bp (12.5%) Read 1: 2,295,342 bp Read 2: 2,223,491 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 71663 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 2 280.1 1 0 2 7 1 4.4 2 1 9 1 0.3 2 0 1 11 1 0.0 3 1 15 1 0.0 4 1 16 1 0.0 4 1 17 1 0.0 5 1 20 15 0.0 6 13 2 21 3 0.0 6 3 22 3 0.0 6 2 1 23 4 0.0 6 4 24 4 0.0 6 4 25 9 0.0 6 9 26 6 0.0 6 6 27 3 0.0 6 3 29 2 0.0 6 2 30 5 0.0 6 5 31 2 0.0 6 2 33 1 0.0 6 1 36 1 0.0 6 1 37 1 0.0 6 1 38 1 0.0 6 1 39 1 0.0 6 0 1 40 1 0.0 6 1 41 2 0.0 6 2 42 3 0.0 6 3 43 2 0.0 6 2 44 6 0.0 6 5 1 45 8 0.0 6 7 1 46 2 0.0 6 2 47 1 0.0 6 1 48 3 0.0 6 2 0 0 0 1 49 2 0.0 6 1 0 0 0 0 1 50 1 0.0 6 1 52 1 0.0 6 1 53 4 0.0 6 4 54 2 0.0 6 2 55 2 0.0 6 2 56 7 0.0 6 7 57 1 0.0 6 1 58 1 0.0 6 1 59 4 0.0 6 3 0 0 0 0 1 60 1 0.0 6 1 62 2 0.0 6 2 63 4 0.0 6 3 1 64 6 0.0 6 6 65 1 0.0 6 0 1 67 1 0.0 6 1 68 1 0.0 6 1 70 1 0.0 6 1 72 2 0.0 6 2 73 3 0.0 6 3 75 2 0.0 6 2 76 1 0.0 6 1 77 2 0.0 6 2 78 1 0.0 6 1 80 1 0.0 6 1 82 1 0.0 6 1 84 2 0.0 6 2 86 9 0.0 6 9 87 6 0.0 6 6 89 4 0.0 6 4 90 6 0.0 6 6 92 6 0.0 6 6 93 1 0.0 6 1 94 1 0.0 6 1 95 2 0.0 6 2 97 9 0.0 6 8 1 98 10 0.0 6 9 1 99 1 0.0 6 0 1 100 7 0.0 6 6 1 101 18 0.0 6 13 4 1 102 6 0.0 6 5 1 103 5 0.0 6 5 104 5 0.0 6 5 105 2 0.0 6 2 106 2 0.0 6 2 107 7 0.0 6 7 108 8 0.0 6 8 109 5 0.0 6 4 0 1 111 5 0.0 6 5 112 5 0.0 6 5 113 6 0.0 6 6 114 1 0.0 6 1 115 13 0.0 6 13 116 2 0.0 6 1 1 117 3 0.0 6 3 118 7 0.0 6 6 1 119 6 0.0 6 5 1 120 5 0.0 6 5 121 12 0.0 6 11 1 122 14 0.0 6 13 1 123 16 0.0 6 15 1 124 10 0.0 6 10 125 5 0.0 6 4 1 126 16 0.0 6 14 2 127 12 0.0 6 10 2 128 20 0.0 6 20 129 7 0.0 6 7 130 12 0.0 6 11 1 131 9 0.0 6 9 132 8 0.0 6 8 133 37 0.0 6 36 1 134 46 0.0 6 46 135 46 0.0 6 46 136 17 0.0 6 17 137 15 0.0 6 15 138 7 0.0 6 7 139 7 0.0 6 7 140 19 0.0 6 19 141 31 0.0 6 29 2 142 24 0.0 6 21 3 143 12 0.0 6 11 1 144 23 0.0 6 22 1 145 44 0.0 6 43 1 146 42 0.0 6 42 147 52 0.0 6 50 2 148 67 0.0 6 67 149 66 0.0 6 61 5 150 54 0.0 6 50 3 1 151 51 0.0 6 51 152 89 0.0 6 88 1 153 47 0.0 6 47 154 29 0.0 6 29 155 17 0.0 6 17 156 13 0.0 6 13 157 31 0.0 6 31 158 19 0.0 6 19 159 13 0.0 6 13 160 33 0.0 6 30 2 0 1 161 24 0.0 6 24 162 35 0.0 6 34 1 163 25 0.0 6 25 164 15 0.0 6 14 1 165 34 0.0 6 33 1 166 28 0.0 6 27 1 167 60 0.0 6 57 3 168 51 0.0 6 51 169 21 0.0 6 20 1 170 44 0.0 6 43 1 171 60 0.0 6 58 2 172 27 0.0 6 27 173 28 0.0 6 28 174 16 0.0 6 14 2 175 17 0.0 6 17 176 16 0.0 6 16 177 18 0.0 6 17 1 178 10 0.0 6 8 1 1 179 27 0.0 6 27 180 73 0.0 6 71 2 181 33 0.0 6 32 1 182 36 0.0 6 33 3 183 28 0.0 6 25 2 1 184 44 0.0 6 41 3 185 69 0.0 6 66 3 186 20 0.0 6 20 187 13 0.0 6 11 2 188 47 0.0 6 45 1 1 189 72 0.0 6 69 2 1 190 113 0.0 6 110 3 191 142 0.0 6 136 4 1 1 192 67 0.0 6 66 1 193 68 0.0 6 67 1 194 87 0.0 6 83 4 195 101 0.0 6 96 5 196 97 0.0 6 97 197 51 0.0 6 49 1 0 0 1 198 77 0.0 6 76 1 199 65 0.0 6 64 1 200 118 0.0 6 115 3 201 127 0.0 6 124 3 202 103 0.0 6 100 3 203 98 0.0 6 93 5 204 97 0.0 6 96 1 205 209 0.0 6 199 10 206 247 0.0 6 243 3 1 207 211 0.0 6 206 5 208 532 0.0 6 518 14 209 232 0.0 6 226 6 210 287 0.0 6 277 10 211 605 0.0 6 587 18 212 139 0.0 6 126 12 1 213 671 0.0 6 660 9 2 214 131 0.0 6 127 4 215 216 0.0 6 210 6 216 136 0.0 6 126 9 1 217 294 0.0 6 285 7 2 218 121 0.0 6 115 5 1 219 321 0.0 6 314 7 220 134 0.0 6 133 1 221 396 0.0 6 392 4 222 183 0.0 6 173 10 223 684 0.0 6 667 16 1 224 384 0.0 6 370 13 1 225 519 0.0 6 495 22 2 226 586 0.0 6 578 8 227 324 0.0 6 321 3 228 380 0.0 6 373 7 229 243 0.0 6 237 6 230 227 0.0 6 218 8 0 1 231 212 0.0 6 207 5 232 171 0.0 6 168 3 233 268 0.0 6 265 3 234 362 0.0 6 355 6 1 235 147 0.0 6 143 3 1 236 323 0.0 6 308 15 237 1227 0.0 6 1214 13 238 184 0.0 6 179 5 239 312 0.0 6 306 4 2 240 214 0.0 6 210 4 241 170 0.0 6 169 1 242 183 0.0 6 177 6 243 593 0.0 6 572 21 244 948 0.0 6 931 17 245 370 0.0 6 362 8 246 263 0.0 6 252 10 1 247 591 0.0 6 581 10 248 491 0.0 6 468 23 249 2041 0.0 6 2003 36 2 250 582 0.0 6 563 19 251 531 0.0 6 516 15 252 905 0.0 6 883 19 3 253 1166 0.0 6 1141 24 1 254 1547 0.0 6 1525 22 255 1158 0.0 6 1113 44 1 256 3181 0.0 6 3130 51 257 945 0.0 6 906 38 1 258 3150 0.0 6 3094 54 2 259 1264 0.0 6 1215 49 260 3734 0.0 6 3677 57 261 418 0.0 6 407 11 262 324 0.0 6 307 17 263 746 0.0 6 725 21 264 1115 0.0 6 1087 28 265 335 0.0 6 324 11 266 1071 0.0 6 1046 24 1 267 401 0.0 6 391 10 268 1010 0.0 6 985 25 269 310 0.0 6 301 9 270 477 0.0 6 462 14 1 271 395 0.0 6 384 11 272 590 0.0 6 573 17 273 1451 0.0 6 1420 31 274 1187 0.0 6 1153 33 0 1 275 3147 0.0 6 3060 86 1 276 4778 0.0 6 4680 94 4 277 2673 0.0 6 2592 81 278 10608 0.0 6 10383 221 4 279 1083 0.0 6 1026 53 0 4 280 38 0.0 6 34 4 281 13 0.0 6 9 1 2 1 282 14 0.0 6 12 1 0 0 0 1 283 2 0.0 6 2 284 2 0.0 6 2 285 3 0.0 6 3 286 1 0.0 6 1 287 4 0.0 6 4 290 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 34 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 7 280.1 1 0 7 63 8 0.0 6 7 1 76 1 0.0 6 1 77 1 0.0 6 1 78 1 0.0 6 1 82 1 0.0 6 1 83 1 0.0 6 1 86 1 0.0 6 1 87 1 0.0 6 1 93 1 0.0 6 1 98 1 0.0 6 1 99 1 0.0 6 1 106 1 0.0 6 1 134 1 0.0 6 1 156 1 0.0 6 1 162 1 0.0 6 1 182 1 0.0 6 1 193 1 0.0 6 1 209 1 0.0 6 1 212 1 0.0 6 1 227 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 71601 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 70.0 1 1 7 1 4.4 2 1 11 5 0.0 3 5 12 7 0.0 3 6 1 13 14 0.0 3 14 14 11 0.0 4 11 15 3 0.0 4 2 1 16 6 0.0 4 5 1 17 11 0.0 5 8 3 18 16 0.0 5 15 1 19 4 0.0 5 3 1 20 22 0.0 6 21 1 21 36 0.0 6 32 4 22 38 0.0 6 37 1 23 32 0.0 6 31 1 24 20 0.0 6 17 2 1 25 17 0.0 6 17 26 18 0.0 6 15 3 27 11 0.0 6 9 2 28 9 0.0 6 8 1 29 12 0.0 6 9 3 30 14 0.0 6 14 31 5 0.0 6 5 32 6 0.0 6 5 1 33 7 0.0 6 6 1 34 8 0.0 6 8 35 8 0.0 6 7 1 36 7 0.0 6 6 1 37 9 0.0 6 8 0 1 38 8 0.0 6 5 3 39 15 0.0 6 14 1 40 12 0.0 6 11 1 41 11 0.0 6 10 1 42 2 0.0 6 2 43 8 0.0 6 7 1 44 17 0.0 6 15 2 45 16 0.0 6 14 2 46 31 0.0 6 26 5 47 28 0.0 6 25 3 48 18 0.0 6 16 2 49 16 0.0 6 16 50 19 0.0 6 13 6 51 11 0.0 6 10 1 52 24 0.0 6 22 2 53 22 0.0 6 20 2 54 14 0.0 6 12 1 1 55 21 0.0 6 19 2 56 16 0.0 6 14 1 1 57 23 0.0 6 21 2 58 12 0.0 6 11 1 59 14 0.0 6 12 2 60 13 0.0 6 11 1 1 61 10 0.0 6 9 1 62 7 0.0 6 7 63 3 0.0 6 3 64 9 0.0 6 8 1 65 6 0.0 6 3 3 66 10 0.0 6 9 1 67 37 0.0 6 36 1 68 119 0.0 6 113 6 69 25 0.0 6 23 2 70 42 0.0 6 40 2 71 24 0.0 6 19 5 72 56 0.0 6 56 73 7 0.0 6 7 74 11 0.0 6 10 1 75 13 0.0 6 11 2 76 21 0.0 6 20 1 77 16 0.0 6 13 2 1 78 27 0.0 6 24 2 1 79 20 0.0 6 20 80 20 0.0 6 17 3 81 13 0.0 6 10 3 82 18 0.0 6 15 3 83 79 0.0 6 75 3 1 84 47 0.0 6 46 0 0 0 1 85 27 0.0 6 25 2 86 24 0.0 6 20 4 87 46 0.0 6 43 3 88 19 0.0 6 16 3 89 14 0.0 6 12 2 90 34 0.0 6 29 3 2 91 31 0.0 6 26 5 92 20 0.0 6 20 93 31 0.0 6 27 4 94 83 0.0 6 76 6 0 1 95 37 0.0 6 36 1 96 40 0.0 6 38 2 97 25 0.0 6 23 2 98 36 0.0 6 34 1 1 99 63 0.0 6 61 1 1 100 36 0.0 6 31 3 1 0 1 101 39 0.0 6 36 3 102 62 0.0 6 58 4 103 71 0.0 6 68 2 1 104 53 0.0 6 50 3 105 106 0.0 6 96 8 1 1 106 35 0.0 6 33 2 107 40 0.0 6 35 4 1 108 36 0.0 6 33 2 0 0 0 1 109 43 0.0 6 40 3 110 43 0.0 6 42 1 111 41 0.0 6 37 3 1 112 25 0.0 6 21 3 1 113 39 0.0 6 37 1 1 114 51 0.0 6 47 4 115 53 0.0 6 51 2 116 66 0.0 6 64 2 117 31 0.0 6 30 0 1 118 50 0.0 6 47 3 119 64 0.0 6 61 3 120 76 0.0 6 73 3 121 29 0.0 6 28 1 122 52 0.0 6 48 3 1 123 53 0.0 6 50 3 124 79 0.0 6 76 3 125 62 0.0 6 56 6 126 49 0.0 6 46 3 127 71 0.0 6 66 5 128 47 0.0 6 43 4 129 45 0.0 6 44 1 130 47 0.0 6 43 4 131 110 0.0 6 108 2 132 148 0.0 6 143 3 1 0 1 133 91 0.0 6 85 5 1 134 167 0.0 6 163 3 1 135 326 0.0 6 312 13 1 136 155 0.0 6 152 3 137 188 0.0 6 180 8 138 63 0.0 6 63 139 129 0.0 6 121 8 140 311 0.0 6 303 8 141 250 0.0 6 241 8 1 142 300 0.0 6 290 10 143 275 0.0 6 266 8 1 144 457 0.0 6 435 20 2 145 256 0.0 6 245 8 2 1 146 267 0.0 6 258 9 147 275 0.0 6 259 16 148 284 0.0 6 272 11 0 1 149 350 0.0 6 332 18 150 422 0.0 6 409 11 0 0 1 0 1 151 433 0.0 6 422 10 1 152 280 0.0 6 271 9 153 301 0.0 6 293 7 1 154 367 0.0 6 355 12 155 180 0.0 6 169 10 1 156 281 0.0 6 269 11 1 157 397 0.0 6 382 14 1 158 462 0.0 6 443 18 0 0 1 159 218 0.0 6 209 9 160 355 0.0 6 348 6 1 161 225 0.0 6 219 6 162 175 0.0 6 170 5 163 397 0.0 6 379 18 164 406 0.0 6 394 11 1 165 450 0.0 6 431 19 166 648 0.0 6 633 14 1 167 447 0.0 6 429 18 168 878 0.0 6 850 25 1 0 2 169 337 0.0 6 323 14 170 416 0.0 6 397 17 1 1 171 586 0.0 6 572 14 172 237 0.0 6 223 12 1 0 1 173 330 0.0 6 321 8 1 174 487 0.0 6 469 15 1 1 1 175 901 0.0 6 872 27 1 1 176 625 0.0 6 588 34 1 0 2 177 956 0.0 6 919 34 2 0 1 178 679 0.0 6 662 15 1 1 179 887 0.0 6 862 24 1 180 792 0.0 6 765 26 0 1 181 1085 0.0 6 1052 32 0 0 1 182 255 0.0 6 249 6 183 1008 0.0 6 980 28 184 894 0.0 6 864 26 1 1 2 185 685 0.0 6 653 29 3 186 1995 0.0 6 1930 63 1 0 1 187 1469 0.0 6 1426 41 2 188 1717 0.0 6 1661 53 2 0 1 189 818 0.0 6 786 32 190 1090 0.0 6 1057 32 1 191 899 0.0 6 888 10 1 192 910 0.0 6 862 45 0 2 1 193 1603 0.0 6 1561 40 2 194 523 0.0 6 501 22 195 1153 0.0 6 1126 25 0 1 1 196 409 0.0 6 399 10 197 1326 0.0 6 1290 34 1 0 1 198 1921 0.0 6 1873 44 4 199 1405 0.0 6 1370 32 2 0 1 200 923 0.0 6 899 24 201 989 0.0 6 955 30 3 0 1 202 930 0.0 6 909 19 1 1 203 742 0.0 6 718 23 1 204 586 0.0 6 562 23 0 0 1 205 1115 0.0 6 1095 19 0 0 1 206 220 0.0 6 209 9 1 1 207 702 0.0 6 678 23 1 208 1123 0.0 6 1094 28 0 0 1 209 818 0.0 6 795 22 1 210 208 0.0 6 199 6 2 0 1 211 430 0.0 6 419 9 1 1 212 592 0.0 6 572 19 0 0 1 213 1157 0.0 6 1132 23 2 214 401 0.0 6 393 6 0 1 1 215 609 0.0 6 593 16 216 399 0.0 6 388 11 217 365 0.0 6 360 5 218 384 0.0 6 377 6 0 0 1 219 320 0.0 6 315 5 220 155 0.0 6 149 6 221 112 0.0 6 108 3 1 222 308 0.0 6 294 13 0 1 223 800 0.0 6 775 22 0 1 1 0 1 224 1480 0.0 6 1453 27 225 885 0.0 6 857 27 1 226 729 0.0 6 697 31 0 0 1 227 2920 0.0 6 2855 60 1 1 3 228 764 0.0 6 743 21 229 1482 0.0 6 1457 24 1 230 110 0.0 6 106 3 1 231 351 0.0 6 342 8 0 0 1 232 441 0.0 6 430 11 233 668 0.0 6 652 16 234 451 0.0 6 444 7 235 260 0.0 6 253 7 236 127 0.0 6 124 3 237 179 0.0 6 175 4 238 216 0.0 6 210 6 239 104 0.0 6 100 4 240 31 0.0 6 29 1 0 0 1 241 36 0.0 6 34 2 242 425 0.0 6 405 18 0 0 1 0 1 243 280 0.0 6 273 6 0 0 1 244 168 0.0 6 164 4 245 114 0.0 6 109 4 0 0 1 246 169 0.0 6 163 5 1 247 119 0.0 6 115 4 248 189 0.0 6 180 9 249 273 0.0 6 267 6 250 56 0.0 6 55 1 251 168 0.0 6 164 4 252 297 0.0 6 290 7 253 30 0.0 6 25 5 254 22 0.0 6 19 3 255 6 0.0 6 6 256 3 0.0 6 3 257 1 0.0 6 1 260 1 0.0 6 1 263 1 0.0 6 1 264 1 0.0 6 1 277 1 0.0 6 1 278 3 0.0 6 3 279 14 0.0 6 12 2 280 21 0.0 6 15 5 0 1 281 2 0.0 6 1 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 96 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 6 71 17.5 1 0 0 71 79 1 0.0 6 1 80 1 0.0 6 1 81 3 0.0 6 3 84 1 0.0 6 1 85 1 0.0 6 1 86 1 0.0 6 1 87 1 0.0 6 1 90 2 0.0 6 2 92 1 0.0 6 1 93 1 0.0 6 1 96 1 0.0 6 1 99 1 0.0 6 1 105 1 0.0 6 1 106 1 0.0 6 1 116 1 0.0 6 1 125 1 0.0 6 1 138 1 0.0 6 1 141 1 0.0 6 1 171 1 0.0 6 1 176 1 0.0 6 1 181 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_09_215_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_09_442_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_09_215_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_09_442_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.78 s (40 µs/read; 1.51 M reads/minute). === Summary === Total read pairs processed: 69,877 Read 1 with adapter: 69,877 (100.0%) Read 2 with adapter: 69,854 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 23 (0.0%) Pairs written (passing filters): 69,854 (100.0%) Total basepairs processed: 35,877,121 bp Read 1: 20,421,795 bp Read 2: 15,455,326 bp Total written (filtered): 4,261,857 bp (11.9%) Read 1: 2,235,351 bp Read 2: 2,026,506 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 69856 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 273.0 1 0 1 6 3 17.1 1 0 0 3 7 4 4.3 2 3 0 1 8 1 1.1 2 1 15 2 0.0 4 2 16 1 0.0 4 1 19 1 0.0 5 1 20 9 0.0 6 9 21 4 0.0 6 4 22 1 0.0 6 1 23 2 0.0 6 2 24 3 0.0 6 3 25 4 0.0 6 4 26 1 0.0 6 1 27 28 0.0 6 27 1 28 10 0.0 6 9 1 29 1 0.0 6 1 30 1 0.0 6 0 1 32 4 0.0 6 4 33 2 0.0 6 2 34 1 0.0 6 1 35 1 0.0 6 1 36 1 0.0 6 1 37 4 0.0 6 4 38 6 0.0 6 5 1 39 1 0.0 6 1 40 3 0.0 6 2 0 0 0 0 1 41 5 0.0 6 3 2 42 1 0.0 6 1 43 1 0.0 6 1 44 6 0.0 6 6 45 3 0.0 6 3 47 2 0.0 6 2 48 2 0.0 6 2 49 10 0.0 6 10 50 9 0.0 6 8 1 51 1 0.0 6 1 52 2 0.0 6 2 54 3 0.0 6 3 55 1 0.0 6 0 1 56 7 0.0 6 7 57 8 0.0 6 7 1 58 4 0.0 6 3 0 1 60 1 0.0 6 1 62 3 0.0 6 3 63 6 0.0 6 5 1 64 2 0.0 6 2 65 5 0.0 6 5 66 3 0.0 6 3 67 1 0.0 6 1 68 6 0.0 6 5 1 69 1 0.0 6 0 1 70 1 0.0 6 1 72 8 0.0 6 8 73 1 0.0 6 1 74 2 0.0 6 2 75 3 0.0 6 3 76 1 0.0 6 1 77 4 0.0 6 4 78 3 0.0 6 3 80 1 0.0 6 1 81 1 0.0 6 1 85 2 0.0 6 2 86 7 0.0 6 7 87 4 0.0 6 4 89 4 0.0 6 4 90 4 0.0 6 3 1 91 1 0.0 6 1 92 4 0.0 6 3 1 93 6 0.0 6 6 94 3 0.0 6 3 95 1 0.0 6 1 96 1 0.0 6 1 97 1 0.0 6 1 98 7 0.0 6 7 100 2 0.0 6 1 1 101 7 0.0 6 5 2 103 3 0.0 6 3 104 2 0.0 6 2 105 4 0.0 6 4 106 3 0.0 6 3 107 2 0.0 6 2 108 9 0.0 6 7 0 2 109 4 0.0 6 3 1 110 1 0.0 6 0 1 111 4 0.0 6 4 112 5 0.0 6 5 113 8 0.0 6 8 114 4 0.0 6 3 0 1 115 7 0.0 6 7 116 6 0.0 6 6 117 3 0.0 6 3 118 4 0.0 6 4 119 7 0.0 6 7 120 6 0.0 6 6 121 5 0.0 6 3 2 122 9 0.0 6 7 2 123 10 0.0 6 9 1 124 8 0.0 6 8 125 7 0.0 6 6 1 126 6 0.0 6 6 127 3 0.0 6 3 128 9 0.0 6 9 129 7 0.0 6 6 1 130 8 0.0 6 8 131 7 0.0 6 6 1 132 3 0.0 6 3 133 5 0.0 6 5 134 16 0.0 6 14 2 135 16 0.0 6 16 136 8 0.0 6 7 1 137 15 0.0 6 15 138 8 0.0 6 8 139 10 0.0 6 10 140 21 0.0 6 19 2 141 15 0.0 6 15 142 15 0.0 6 15 143 29 0.0 6 28 1 144 11 0.0 6 11 145 35 0.0 6 34 1 146 23 0.0 6 22 1 147 9 0.0 6 9 148 14 0.0 6 14 149 23 0.0 6 22 1 150 30 0.0 6 30 151 13 0.0 6 11 1 1 152 26 0.0 6 24 2 153 28 0.0 6 28 154 20 0.0 6 20 155 7 0.0 6 7 156 18 0.0 6 16 1 0 1 157 11 0.0 6 11 158 27 0.0 6 27 159 23 0.0 6 22 1 160 19 0.0 6 19 161 19 0.0 6 19 162 11 0.0 6 10 1 163 45 0.0 6 43 2 164 16 0.0 6 15 1 165 62 0.0 6 59 3 166 23 0.0 6 21 2 167 36 0.0 6 35 1 168 40 0.0 6 40 169 29 0.0 6 28 1 170 14 0.0 6 14 171 46 0.0 6 42 4 172 21 0.0 6 21 173 22 0.0 6 21 1 174 42 0.0 6 38 4 175 53 0.0 6 51 2 176 45 0.0 6 43 2 177 42 0.0 6 41 1 178 38 0.0 6 35 2 0 0 0 0 1 179 57 0.0 6 54 2 1 180 39 0.0 6 38 1 181 28 0.0 6 27 1 182 36 0.0 6 36 183 95 0.0 6 90 5 184 68 0.0 6 66 2 185 93 0.0 6 90 3 186 135 0.0 6 133 2 187 49 0.0 6 45 3 1 188 67 0.0 6 63 4 189 126 0.0 6 126 190 90 0.0 6 87 3 191 121 0.0 6 120 1 192 114 0.0 6 111 3 193 138 0.0 6 131 7 194 138 0.0 6 137 1 195 154 0.0 6 150 4 196 173 0.0 6 166 7 197 143 0.0 6 139 3 0 1 198 182 0.0 6 179 3 199 100 0.0 6 97 3 200 83 0.0 6 81 2 201 134 0.0 6 124 9 0 0 1 202 148 0.0 6 146 1 0 1 203 171 0.0 6 168 3 204 114 0.0 6 107 5 0 1 1 205 179 0.0 6 178 1 206 152 0.0 6 146 6 207 146 0.0 6 143 3 208 244 0.0 6 238 6 209 164 0.0 6 159 5 210 138 0.0 6 133 5 211 96 0.0 6 95 1 212 102 0.0 6 101 1 213 163 0.0 6 162 1 214 192 0.0 6 192 215 161 0.0 6 159 2 216 183 0.0 6 180 2 1 217 270 0.0 6 264 6 218 190 0.0 6 189 1 219 235 0.0 6 231 4 220 178 0.0 6 175 3 221 191 0.0 6 186 5 222 210 0.0 6 208 2 223 94 0.0 6 93 1 224 79 0.0 6 76 3 225 174 0.0 6 169 5 226 395 0.0 6 388 7 227 272 0.0 6 270 2 228 285 0.0 6 278 7 229 220 0.0 6 215 4 0 1 230 214 0.0 6 212 2 231 167 0.0 6 162 5 232 197 0.0 6 194 3 233 228 0.0 6 223 5 234 229 0.0 6 227 2 235 134 0.0 6 129 5 236 112 0.0 6 109 3 237 166 0.0 6 161 5 238 204 0.0 6 198 5 1 239 212 0.0 6 207 5 240 248 0.0 6 240 7 1 241 443 0.0 6 436 7 242 394 0.0 6 382 11 0 1 243 355 0.0 6 350 4 0 0 1 244 395 0.0 6 385 10 245 532 0.0 6 522 9 0 0 1 246 288 0.0 6 285 3 247 224 0.0 6 220 4 248 193 0.0 6 188 3 0 2 249 428 0.0 6 421 7 250 294 0.0 6 286 7 1 251 404 0.0 6 394 10 252 297 0.0 6 287 9 1 253 387 0.0 6 381 6 254 655 0.0 6 638 16 1 255 440 0.0 6 432 7 1 256 354 0.0 6 346 8 257 348 0.0 6 338 10 258 752 0.0 6 735 16 0 1 259 1238 0.0 6 1209 27 1 1 260 1143 0.0 6 1124 18 0 0 1 261 490 0.0 6 470 20 262 654 0.0 6 632 22 263 1496 0.0 6 1462 31 2 1 264 1415 0.0 6 1383 31 0 1 265 931 0.0 6 903 28 266 1776 0.0 6 1740 34 0 2 267 653 0.0 6 638 15 268 1307 0.0 6 1276 27 2 1 1 269 1037 0.0 6 1006 31 270 1099 0.0 6 1075 23 1 271 885 0.0 6 868 16 0 1 272 1132 0.0 6 1109 20 3 273 982 0.0 6 950 31 0 0 1 274 2696 0.0 6 2642 52 0 2 275 1704 0.0 6 1659 42 1 2 276 3052 0.0 6 2980 67 2 3 277 5963 0.0 6 5843 116 2 2 278 9330 0.0 6 9090 221 4 14 1 279 12304 0.0 6 11965 320 15 4 280 166 0.0 6 143 19 4 281 28 0.0 6 25 1 1 1 282 18 0.0 6 14 1 3 283 9 0.0 6 7 1 0 0 1 284 6 0.0 6 6 285 1 0.0 6 1 286 2 0.0 6 2 287 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 21 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 6 273.0 1 0 6 81 1 0.0 6 1 102 1 0.0 6 1 114 1 0.0 6 1 115 1 0.0 6 1 117 1 0.0 6 1 118 1 0.0 6 1 126 1 0.0 6 1 130 1 0.0 6 1 132 1 0.0 6 1 133 2 0.0 6 2 137 1 0.0 6 1 140 1 0.0 6 1 141 1 0.0 6 1 212 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 69830 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 5 17.1 1 3 2 7 2 4.3 2 2 9 2 0.3 2 2 11 1 0.0 3 1 12 4 0.0 3 3 1 13 3 0.0 3 2 1 14 6 0.0 4 6 15 1 0.0 4 1 16 3 0.0 4 3 17 14 0.0 5 12 2 18 1 0.0 5 1 19 23 0.0 5 23 20 63 0.0 6 63 21 44 0.0 6 41 3 22 30 0.0 6 29 1 23 17 0.0 6 15 2 24 23 0.0 6 21 2 25 12 0.0 6 12 26 12 0.0 6 12 27 14 0.0 6 13 1 28 14 0.0 6 13 1 29 13 0.0 6 11 1 0 0 1 30 23 0.0 6 20 3 31 5 0.0 6 5 32 7 0.0 6 7 33 15 0.0 6 13 2 34 15 0.0 6 15 35 14 0.0 6 13 0 1 36 6 0.0 6 6 37 9 0.0 6 9 38 10 0.0 6 10 39 10 0.0 6 9 1 40 9 0.0 6 9 41 6 0.0 6 6 42 3 0.0 6 3 43 10 0.0 6 7 3 44 9 0.0 6 9 45 8 0.0 6 7 1 46 13 0.0 6 12 1 47 13 0.0 6 12 1 48 13 0.0 6 13 49 26 0.0 6 21 5 50 11 0.0 6 10 1 51 19 0.0 6 16 3 52 18 0.0 6 15 2 0 0 1 53 12 0.0 6 10 2 54 14 0.0 6 13 1 55 21 0.0 6 18 2 1 56 28 0.0 6 25 1 2 57 21 0.0 6 17 4 58 19 0.0 6 18 1 59 17 0.0 6 17 60 16 0.0 6 15 1 61 9 0.0 6 8 1 62 14 0.0 6 13 1 63 22 0.0 6 20 2 64 15 0.0 6 14 1 65 6 0.0 6 6 66 18 0.0 6 17 1 67 11 0.0 6 10 1 68 11 0.0 6 11 69 18 0.0 6 18 70 125 0.0 6 117 8 71 35 0.0 6 31 4 72 70 0.0 6 66 4 73 16 0.0 6 16 74 22 0.0 6 21 1 75 17 0.0 6 16 1 76 19 0.0 6 18 1 77 12 0.0 6 11 1 78 17 0.0 6 15 2 79 8 0.0 6 7 1 80 28 0.0 6 26 1 0 1 81 22 0.0 6 20 1 0 1 82 18 0.0 6 16 2 83 28 0.0 6 27 1 84 65 0.0 6 60 4 0 0 1 85 54 0.0 6 50 4 86 34 0.0 6 33 0 0 0 1 87 37 0.0 6 36 1 88 16 0.0 6 13 3 89 17 0.0 6 17 90 26 0.0 6 24 1 0 0 1 91 26 0.0 6 25 1 92 16 0.0 6 15 1 93 28 0.0 6 26 1 0 0 1 94 64 0.0 6 60 3 0 0 1 95 31 0.0 6 26 4 1 96 36 0.0 6 29 5 2 97 30 0.0 6 30 98 22 0.0 6 18 2 1 1 99 51 0.0 6 45 3 1 0 2 100 37 0.0 6 34 2 0 0 0 1 101 35 0.0 6 32 2 1 102 56 0.0 6 54 2 103 65 0.0 6 58 7 104 71 0.0 6 65 6 105 59 0.0 6 55 3 1 106 52 0.0 6 48 3 1 107 74 0.0 6 70 4 108 62 0.0 6 58 4 109 41 0.0 6 37 2 1 0 1 110 24 0.0 6 23 1 111 42 0.0 6 40 2 112 42 0.0 6 39 3 113 39 0.0 6 37 2 114 25 0.0 6 23 2 115 53 0.0 6 53 116 42 0.0 6 38 4 117 27 0.0 6 24 2 0 0 1 118 46 0.0 6 45 1 119 48 0.0 6 47 1 120 84 0.0 6 80 3 1 121 32 0.0 6 29 3 122 94 0.0 6 90 4 123 69 0.0 6 66 1 2 124 74 0.0 6 73 1 125 45 0.0 6 39 3 1 1 0 1 126 31 0.0 6 30 1 127 62 0.0 6 58 3 1 128 66 0.0 6 63 2 1 129 63 0.0 6 57 5 1 130 81 0.0 6 78 3 131 75 0.0 6 73 2 132 101 0.0 6 93 8 133 88 0.0 6 84 3 0 0 1 134 166 0.0 6 159 5 2 135 189 0.0 6 183 5 0 0 1 136 122 0.0 6 113 8 0 1 137 116 0.0 6 112 3 1 138 91 0.0 6 86 5 139 90 0.0 6 84 4 2 140 101 0.0 6 94 7 141 114 0.0 6 108 5 1 142 209 0.0 6 205 3 0 0 1 143 179 0.0 6 170 9 144 245 0.0 6 230 14 1 145 177 0.0 6 165 12 146 233 0.0 6 224 9 147 120 0.0 6 117 3 148 125 0.0 6 119 6 149 338 0.0 6 328 10 150 293 0.0 6 280 10 3 151 328 0.0 6 314 14 152 413 0.0 6 394 18 0 0 0 1 153 349 0.0 6 337 11 1 154 270 0.0 6 258 11 1 155 352 0.0 6 341 11 156 392 0.0 6 380 11 0 0 0 1 157 361 0.0 6 348 13 158 442 0.0 6 425 16 0 0 0 1 159 239 0.0 6 227 12 160 322 0.0 6 312 10 161 264 0.0 6 254 9 1 162 338 0.0 6 323 13 1 0 1 163 393 0.0 6 382 10 0 1 164 332 0.0 6 321 11 165 437 0.0 6 424 12 0 0 1 166 569 0.0 6 548 15 5 1 167 382 0.0 6 366 14 1 1 168 621 0.0 6 609 12 169 312 0.0 6 303 9 170 436 0.0 6 421 15 171 595 0.0 6 575 18 1 0 1 172 315 0.0 6 300 15 173 569 0.0 6 544 21 2 0 1 1 174 711 0.0 6 677 32 1 0 1 175 912 0.0 6 880 28 3 0 1 176 432 0.0 6 415 17 177 471 0.0 6 457 13 0 1 178 361 0.0 6 348 12 0 0 1 179 343 0.0 6 332 9 2 180 404 0.0 6 390 13 0 0 1 181 654 0.0 6 629 23 2 182 437 0.0 6 423 12 1 0 1 183 612 0.0 6 596 16 184 709 0.0 6 691 16 1 0 1 185 211 0.0 6 195 15 1 186 825 0.0 6 796 28 1 187 1232 0.0 6 1202 27 2 0 1 188 1027 0.0 6 999 27 1 189 759 0.0 6 735 22 1 0 1 190 1268 0.0 6 1220 48 191 915 0.0 6 891 24 192 786 0.0 6 750 34 2 193 615 0.0 6 599 16 194 969 0.0 6 934 31 2 1 1 195 971 0.0 6 942 26 2 1 196 1800 0.0 6 1763 35 0 1 1 197 622 0.0 6 599 22 1 198 1032 0.0 6 985 45 1 0 1 199 2112 0.0 6 2055 56 0 0 1 200 1176 0.0 6 1142 32 1 0 0 0 1 201 1564 0.0 6 1522 41 0 1 202 966 0.0 6 936 28 2 203 1043 0.0 6 1005 35 2 0 0 0 1 204 1050 0.0 6 1035 15 205 555 0.0 6 527 27 0 0 1 206 1682 0.0 6 1638 42 2 207 520 0.0 6 509 11 208 780 0.0 6 760 18 1 0 0 1 209 1581 0.0 6 1545 35 0 0 1 210 884 0.0 6 856 25 2 0 1 211 925 0.0 6 901 23 1 212 902 0.0 6 867 32 2 0 1 213 582 0.0 6 559 22 0 0 1 214 394 0.0 6 380 13 1 215 510 0.0 6 495 12 3 216 435 0.0 6 423 12 217 843 0.0 6 813 29 1 218 718 0.0 6 698 18 1 0 1 219 629 0.0 6 613 16 220 682 0.0 6 660 21 0 1 221 942 0.0 6 920 20 2 222 482 0.0 6 464 16 2 223 1328 0.0 6 1280 45 0 0 2 1 224 2431 0.0 6 2369 61 1 225 519 0.0 6 497 21 1 226 765 0.0 6 732 29 3 1 227 783 0.0 6 765 16 2 228 303 0.0 6 294 9 229 1389 0.0 6 1353 35 1 230 246 0.0 6 237 9 231 289 0.0 6 276 12 0 1 232 769 0.0 6 754 14 0 1 233 313 0.0 6 302 10 1 234 439 0.0 6 424 14 0 0 1 235 373 0.0 6 367 6 236 282 0.0 6 274 7 1 237 127 0.0 6 121 6 238 140 0.0 6 137 1 1 0 1 239 35 0.0 6 35 240 120 0.0 6 116 4 241 419 0.0 6 408 11 242 237 0.0 6 231 6 243 265 0.0 6 261 4 244 346 0.0 6 341 4 1 245 76 0.0 6 76 246 49 0.0 6 48 1 247 119 0.0 6 111 6 1 0 1 248 77 0.0 6 73 3 1 249 95 0.0 6 89 6 250 25 0.0 6 25 251 149 0.0 6 147 2 252 111 0.0 6 107 4 253 327 0.0 6 319 8 254 65 0.0 6 64 1 255 7 0.0 6 5 2 256 11 0.0 6 10 1 257 12 0.0 6 12 258 3 0.0 6 3 259 1 0.0 6 1 263 1 0.0 6 1 269 1 0.0 6 1 276 2 0.0 6 2 280 4 0.0 6 4 281 34 0.0 6 34 282 23 0.0 6 22 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 24 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 88 1 0.0 6 1 92 1 0.0 6 1 98 1 0.0 6 1 100 1 0.0 6 1 108 2 0.0 6 2 112 3 0.0 6 3 115 1 0.0 6 1 122 2 0.0 6 2 124 1 0.0 6 1 130 1 0.0 6 1 134 1 0.0 6 1 137 1 0.0 6 1 148 1 0.0 6 1 151 2 0.0 6 2 153 1 0.0 6 1 169 1 0.0 6 1 179 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_12_204_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_12_431_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_12_204_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_12_431_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_13_205_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_21-Rev_13_432_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_13_205_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_21-Rev_13_432_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_07_206_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_07_433_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_07_206_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_07_433_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_08_207_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_08_434_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_08_207_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_08_434_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_09_208_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_09_435_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_09_208_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_09_435_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_10_209_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_10_436_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_10_209_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_10_436_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_11_210_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_11_437_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_11_210_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_11_437_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_12_211_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_12_438_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_12_211_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_12_438_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_13_212_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_22-Rev_13_439_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_13_212_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_22-Rev_13_439_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_07_213_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_07_440_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_07_213_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_07_440_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_08_214_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_08_441_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_08_214_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_08_441_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_09_215_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_09_442_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_09_215_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_09_442_L001_R2_001.fastq.gz Command: This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_10_216_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_10_443_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_10_216_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_10_443_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.08 s (45 µs/read; 1.34 M reads/minute). === Summary === Total read pairs processed: 46,414 Read 1 with adapter: 46,414 (100.0%) Read 2 with adapter: 46,380 (99.9%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 34 (0.1%) Pairs written (passing filters): 46,380 (99.9%) Total basepairs processed: 22,926,067 bp Read 1: 13,104,584 bp Read 2: 9,821,483 bp Total written (filtered): 2,971,351 bp (13.0%) Read 1: 1,486,303 bp Read 2: 1,485,048 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 46366 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 725.2 0 0 1 4 1 181.3 1 0 1 6 2 11.3 1 0 0 2 7 2 2.8 2 0 0 2 8 1 0.7 2 0 1 16 1 0.0 4 0 1 17 1 0.0 5 1 20 7 0.0 6 7 21 5 0.0 6 5 22 3 0.0 6 3 23 3 0.0 6 3 25 10 0.0 6 10 26 3 0.0 6 3 27 2 0.0 6 1 1 29 4 0.0 6 2 1 1 30 3 0.0 6 3 32 2 0.0 6 2 33 1 0.0 6 1 34 1 0.0 6 1 35 1 0.0 6 0 1 37 1 0.0 6 1 38 3 0.0 6 3 39 2 0.0 6 1 0 1 40 4 0.0 6 4 42 1 0.0 6 1 45 6 0.0 6 6 46 3 0.0 6 3 47 2 0.0 6 1 1 48 2 0.0 6 2 51 1 0.0 6 0 1 52 1 0.0 6 1 54 1 0.0 6 1 56 2 0.0 6 2 57 2 0.0 6 0 2 62 4 0.0 6 3 1 64 1 0.0 6 1 65 4 0.0 6 4 66 1 0.0 6 1 67 3 0.0 6 3 68 1 0.0 6 1 69 1 0.0 6 1 70 1 0.0 6 0 0 1 71 3 0.0 6 2 1 72 1 0.0 6 1 74 2 0.0 6 2 76 1 0.0 6 1 77 3 0.0 6 3 78 7 0.0 6 6 1 79 1 0.0 6 1 81 3 0.0 6 3 83 2 0.0 6 2 84 4 0.0 6 4 86 8 0.0 6 7 1 87 6 0.0 6 6 88 1 0.0 6 1 89 2 0.0 6 2 90 7 0.0 6 7 91 6 0.0 6 5 1 92 3 0.0 6 3 93 5 0.0 6 5 94 3 0.0 6 3 97 6 0.0 6 5 0 0 1 98 8 0.0 6 7 1 99 5 0.0 6 5 100 8 0.0 6 8 101 10 0.0 6 8 2 102 3 0.0 6 2 1 103 8 0.0 6 8 104 3 0.0 6 3 105 4 0.0 6 3 1 106 1 0.0 6 0 1 107 6 0.0 6 5 1 108 7 0.0 6 7 109 4 0.0 6 4 110 2 0.0 6 2 111 3 0.0 6 3 112 12 0.0 6 11 1 113 9 0.0 6 8 1 114 10 0.0 6 10 115 12 0.0 6 11 1 116 1 0.0 6 1 117 6 0.0 6 6 118 7 0.0 6 7 119 8 0.0 6 8 120 3 0.0 6 3 121 7 0.0 6 7 122 7 0.0 6 5 2 123 12 0.0 6 11 1 124 7 0.0 6 7 125 9 0.0 6 8 1 126 12 0.0 6 12 127 8 0.0 6 7 1 128 9 0.0 6 8 1 129 11 0.0 6 11 130 6 0.0 6 6 131 11 0.0 6 11 132 17 0.0 6 16 1 133 12 0.0 6 10 2 134 26 0.0 6 25 0 0 1 135 22 0.0 6 22 136 6 0.0 6 6 137 17 0.0 6 17 138 14 0.0 6 14 139 15 0.0 6 14 1 140 15 0.0 6 15 141 13 0.0 6 12 1 142 25 0.0 6 24 1 143 38 0.0 6 38 144 45 0.0 6 44 1 145 70 0.0 6 70 146 41 0.0 6 40 1 147 53 0.0 6 52 1 148 39 0.0 6 39 149 58 0.0 6 58 150 74 0.0 6 74 151 50 0.0 6 49 1 152 55 0.0 6 54 1 153 52 0.0 6 52 154 56 0.0 6 52 4 155 21 0.0 6 21 156 45 0.0 6 45 157 39 0.0 6 39 158 45 0.0 6 45 159 30 0.0 6 30 160 43 0.0 6 43 161 57 0.0 6 57 162 64 0.0 6 61 3 163 62 0.0 6 61 1 164 45 0.0 6 45 165 43 0.0 6 41 2 166 30 0.0 6 28 1 0 1 167 37 0.0 6 36 1 168 49 0.0 6 45 2 1 1 169 71 0.0 6 68 2 1 170 40 0.0 6 39 1 171 43 0.0 6 42 1 172 39 0.0 6 39 173 43 0.0 6 42 1 174 47 0.0 6 46 1 175 59 0.0 6 56 2 1 176 59 0.0 6 56 3 177 86 0.0 6 86 178 63 0.0 6 62 1 179 49 0.0 6 48 1 180 37 0.0 6 37 181 60 0.0 6 59 1 182 79 0.0 6 78 0 1 183 71 0.0 6 69 2 184 70 0.0 6 70 185 89 0.0 6 87 2 186 89 0.0 6 87 2 187 74 0.0 6 70 4 188 136 0.0 6 136 189 160 0.0 6 157 3 190 99 0.0 6 96 3 191 109 0.0 6 107 2 192 166 0.0 6 161 5 193 173 0.0 6 165 7 0 0 1 194 140 0.0 6 133 7 195 206 0.0 6 199 7 196 246 0.0 6 238 7 1 197 248 0.0 6 241 6 1 198 237 0.0 6 227 10 199 196 0.0 6 190 6 200 161 0.0 6 157 4 201 169 0.0 6 164 5 202 230 0.0 6 222 7 1 203 257 0.0 6 251 6 204 205 0.0 6 196 9 205 238 0.0 6 232 6 206 209 0.0 6 208 1 207 214 0.0 6 209 5 208 274 0.0 6 267 7 209 208 0.0 6 201 6 1 210 188 0.0 6 180 8 211 231 0.0 6 226 5 212 146 0.0 6 143 3 213 156 0.0 6 153 3 214 199 0.0 6 197 2 215 209 0.0 6 206 3 216 190 0.0 6 188 2 217 214 0.0 6 212 2 218 195 0.0 6 192 2 1 219 164 0.0 6 160 4 220 217 0.0 6 213 4 221 163 0.0 6 158 5 222 249 0.0 6 244 5 223 126 0.0 6 122 4 224 112 0.0 6 109 3 225 199 0.0 6 193 6 226 402 0.0 6 394 8 227 244 0.0 6 242 2 228 226 0.0 6 218 8 229 208 0.0 6 206 2 230 198 0.0 6 195 3 231 147 0.0 6 142 5 232 137 0.0 6 136 1 233 146 0.0 6 143 3 234 177 0.0 6 174 3 235 213 0.0 6 206 7 236 173 0.0 6 166 7 237 234 0.0 6 232 2 238 204 0.0 6 201 3 239 336 0.0 6 331 5 240 292 0.0 6 283 8 0 0 0 0 1 241 478 0.0 6 472 6 242 365 0.0 6 358 7 243 445 0.0 6 437 8 244 261 0.0 6 245 16 245 527 0.0 6 518 8 1 246 206 0.0 6 201 5 247 263 0.0 6 256 5 2 248 228 0.0 6 223 5 249 410 0.0 6 405 4 1 250 293 0.0 6 289 3 1 251 376 0.0 6 370 5 1 252 247 0.0 6 243 4 253 245 0.0 6 243 2 254 520 0.0 6 514 6 255 252 0.0 6 247 5 256 277 0.0 6 268 8 0 1 257 441 0.0 6 430 10 1 258 359 0.0 6 352 7 259 408 0.0 6 400 8 260 649 0.0 6 644 5 261 210 0.0 6 203 7 262 187 0.0 6 185 2 263 469 0.0 6 459 10 264 206 0.0 6 199 7 265 309 0.0 6 301 7 0 0 1 266 410 0.0 6 405 5 267 240 0.0 6 235 5 268 243 0.0 6 238 4 0 1 269 543 0.0 6 536 7 270 204 0.0 6 198 6 271 314 0.0 6 304 9 1 272 712 0.0 6 698 14 273 375 0.0 6 368 7 274 1019 0.0 6 1004 15 275 383 0.0 6 367 16 276 1323 0.0 6 1285 37 1 277 3431 0.0 6 3373 57 1 278 2492 0.0 6 2434 57 1 279 12289 0.0 6 11936 345 6 1 1 280 154 0.0 6 135 17 1 1 281 15 0.0 6 14 0 1 282 3 0.0 6 2 0 1 283 3 0.0 6 3 284 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 48 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 3 181.3 1 0 3 72 2 0.0 6 2 75 2 0.0 6 2 76 4 0.0 6 4 77 1 0.0 6 1 80 1 0.0 6 1 81 2 0.0 6 2 82 2 0.0 6 2 84 1 0.0 6 1 92 1 0.0 6 1 93 1 0.0 6 1 94 2 0.0 6 2 98 1 0.0 6 1 100 1 0.0 6 1 105 1 0.0 6 1 106 1 0.0 6 1 112 1 0.0 6 1 118 1 0.0 6 1 119 2 0.0 6 2 120 1 0.0 6 1 122 1 0.0 6 1 128 1 0.0 6 1 130 1 0.0 6 1 131 1 0.0 6 1 137 1 0.0 6 1 139 1 0.0 6 0 1 148 1 0.0 6 1 157 1 0.0 6 1 177 1 0.0 6 1 181 1 0.0 6 1 185 1 0.0 6 1 189 1 0.0 6 1 191 1 0.0 6 1 212 1 0.0 6 1 219 1 0.0 6 1 229 1 0.0 6 1 247 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 46336 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 725.2 0 0 2 6 1 11.3 1 1 9 1 0.2 2 0 1 10 1 0.0 3 1 11 3 0.0 3 3 12 2 0.0 3 2 13 5 0.0 3 5 14 10 0.0 4 8 2 16 7 0.0 4 7 17 14 0.0 5 12 2 18 12 0.0 5 10 2 19 10 0.0 5 10 20 34 0.0 6 33 1 21 39 0.0 6 36 3 22 27 0.0 6 23 4 23 14 0.0 6 12 2 24 16 0.0 6 16 25 22 0.0 6 22 26 13 0.0 6 9 4 27 15 0.0 6 13 2 28 8 0.0 6 8 29 16 0.0 6 15 1 30 17 0.0 6 15 2 31 16 0.0 6 13 3 32 4 0.0 6 4 33 7 0.0 6 5 1 1 34 12 0.0 6 12 35 19 0.0 6 17 1 0 0 1 36 5 0.0 6 4 1 37 9 0.0 6 6 3 38 3 0.0 6 3 39 8 0.0 6 8 40 5 0.0 6 5 41 12 0.0 6 10 2 42 7 0.0 6 4 3 43 15 0.0 6 13 1 1 44 13 0.0 6 10 3 45 2 0.0 6 2 46 21 0.0 6 19 1 1 47 14 0.0 6 14 48 15 0.0 6 13 2 49 17 0.0 6 16 1 50 12 0.0 6 10 2 51 6 0.0 6 5 1 52 22 0.0 6 18 3 0 0 1 53 21 0.0 6 18 3 54 15 0.0 6 13 2 55 17 0.0 6 15 2 56 11 0.0 6 11 57 24 0.0 6 22 1 1 58 14 0.0 6 12 2 59 12 0.0 6 10 2 60 19 0.0 6 16 3 61 15 0.0 6 15 62 7 0.0 6 7 63 9 0.0 6 9 64 10 0.0 6 9 1 65 7 0.0 6 6 1 66 15 0.0 6 11 4 67 92 0.0 6 85 5 2 68 24 0.0 6 22 2 69 42 0.0 6 40 2 70 20 0.0 6 16 3 0 0 1 71 30 0.0 6 28 2 72 16 0.0 6 15 0 1 73 7 0.0 6 7 74 5 0.0 6 4 0 1 75 20 0.0 6 19 1 76 26 0.0 6 24 2 77 13 0.0 6 12 1 78 15 0.0 6 14 0 1 79 13 0.0 6 12 1 80 24 0.0 6 24 81 23 0.0 6 20 3 82 31 0.0 6 29 2 83 43 0.0 6 38 5 84 20 0.0 6 20 85 26 0.0 6 22 3 0 0 1 86 19 0.0 6 18 1 87 33 0.0 6 30 2 0 0 1 88 22 0.0 6 20 2 89 27 0.0 6 23 4 90 28 0.0 6 27 1 91 34 0.0 6 30 4 92 21 0.0 6 19 2 93 41 0.0 6 39 2 94 60 0.0 6 54 5 1 95 36 0.0 6 34 2 96 40 0.0 6 40 97 25 0.0 6 25 98 35 0.0 6 33 2 99 66 0.0 6 62 3 1 100 57 0.0 6 52 4 1 101 39 0.0 6 38 1 102 72 0.0 6 68 2 1 0 1 103 78 0.0 6 74 4 104 48 0.0 6 44 4 105 63 0.0 6 59 3 0 0 1 106 39 0.0 6 36 2 0 0 1 107 30 0.0 6 25 5 108 36 0.0 6 33 2 1 109 45 0.0 6 41 3 1 110 44 0.0 6 39 3 2 111 52 0.0 6 48 4 112 41 0.0 6 41 113 39 0.0 6 36 3 114 61 0.0 6 56 5 115 48 0.0 6 46 2 116 44 0.0 6 42 2 117 50 0.0 6 45 5 118 39 0.0 6 34 4 1 119 60 0.0 6 56 4 120 81 0.0 6 75 5 1 121 52 0.0 6 49 3 122 80 0.0 6 77 3 123 62 0.0 6 60 2 124 98 0.0 6 94 4 125 96 0.0 6 88 8 126 72 0.0 6 70 2 127 105 0.0 6 103 2 128 53 0.0 6 48 5 129 54 0.0 6 52 2 130 61 0.0 6 58 3 131 104 0.0 6 100 4 132 130 0.0 6 125 4 0 0 1 133 98 0.0 6 93 5 134 159 0.0 6 151 5 1 0 0 1 1 135 216 0.0 6 211 4 1 136 97 0.0 6 91 5 0 1 137 136 0.0 6 133 3 138 66 0.0 6 65 1 139 127 0.0 6 121 6 140 259 0.0 6 250 9 141 225 0.0 6 219 6 142 313 0.0 6 305 8 143 245 0.0 6 237 7 0 0 1 144 383 0.0 6 369 12 1 0 1 145 215 0.0 6 210 4 1 146 201 0.0 6 194 7 147 163 0.0 6 157 4 1 0 1 148 206 0.0 6 191 15 149 392 0.0 6 379 12 0 0 1 150 679 0.0 6 657 22 151 237 0.0 6 233 4 152 596 0.0 6 581 13 1 0 1 153 338 0.0 6 323 15 154 318 0.0 6 309 9 155 314 0.0 6 303 9 2 156 235 0.0 6 231 4 157 383 0.0 6 373 10 158 355 0.0 6 348 7 159 163 0.0 6 155 8 160 257 0.0 6 250 6 1 161 165 0.0 6 159 4 1 0 1 162 134 0.0 6 126 8 163 281 0.0 6 275 6 164 296 0.0 6 286 8 1 1 165 373 0.0 6 363 10 166 375 0.0 6 363 11 1 167 279 0.0 6 267 12 168 434 0.0 6 426 8 169 180 0.0 6 168 12 170 213 0.0 6 206 7 171 406 0.0 6 398 8 172 253 0.0 6 240 12 1 173 381 0.0 6 370 10 1 174 625 0.0 6 611 14 175 835 0.0 6 811 21 2 0 0 0 1 176 627 0.0 6 604 22 1 177 580 0.0 6 566 14 178 461 0.0 6 445 15 0 0 1 179 463 0.0 6 448 15 180 331 0.0 6 321 9 1 181 418 0.0 6 403 14 0 0 1 182 141 0.0 6 135 5 1 183 521 0.0 6 505 15 0 1 184 289 0.0 6 279 9 0 1 185 532 0.0 6 515 16 1 186 883 0.0 6 860 21 2 187 884 0.0 6 846 36 0 1 1 188 897 0.0 6 858 35 4 189 692 0.0 6 675 16 1 190 1102 0.0 6 1070 28 1 0 3 191 1281 0.0 6 1248 31 0 1 1 192 653 0.0 6 633 20 193 340 0.0 6 331 8 1 194 806 0.0 6 784 22 195 1022 0.0 6 998 22 1 0 1 196 1844 0.0 6 1793 45 3 1 2 197 576 0.0 6 556 20 198 1114 0.0 6 1077 37 199 1865 0.0 6 1814 48 2 0 1 200 707 0.0 6 688 19 201 1020 0.0 6 993 25 0 0 2 202 511 0.0 6 496 12 1 0 1 0 1 203 425 0.0 6 418 7 204 728 0.0 6 709 18 1 205 217 0.0 6 212 4 1 206 213 0.0 6 209 3 0 1 207 130 0.0 6 127 3 208 226 0.0 6 222 4 209 234 0.0 6 230 4 210 215 0.0 6 212 3 211 179 0.0 6 177 2 212 315 0.0 6 312 3 213 162 0.0 6 158 4 214 480 0.0 6 472 8 215 420 0.0 6 409 11 216 355 0.0 6 345 8 2 217 365 0.0 6 356 8 1 218 271 0.0 6 267 4 219 408 0.0 6 397 11 220 95 0.0 6 94 1 221 352 0.0 6 337 14 1 222 342 0.0 6 336 5 1 223 434 0.0 6 428 6 224 249 0.0 6 235 14 225 287 0.0 6 276 11 226 215 0.0 6 206 9 227 196 0.0 6 192 4 228 134 0.0 6 133 1 229 280 0.0 6 272 7 0 0 1 230 110 0.0 6 107 3 231 159 0.0 6 156 3 232 196 0.0 6 192 4 233 135 0.0 6 132 2 1 234 99 0.0 6 99 235 47 0.0 6 47 236 14 0.0 6 14 237 19 0.0 6 17 2 238 20 0.0 6 20 239 20 0.0 6 19 1 240 40 0.0 6 38 2 241 89 0.0 6 88 1 242 117 0.0 6 116 1 243 72 0.0 6 71 0 1 244 62 0.0 6 61 1 245 64 0.0 6 61 3 246 69 0.0 6 69 247 24 0.0 6 24 248 47 0.0 6 45 2 249 13 0.0 6 13 250 14 0.0 6 14 251 21 0.0 6 21 252 5 0.0 6 5 254 2 0.0 6 2 258 1 0.0 6 1 276 1 0.0 6 1 277 1 0.0 6 1 278 16 0.0 6 16 279 27 0.0 6 26 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 44 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 75 2 0.0 6 2 76 1 0.0 6 1 77 1 0.0 6 1 78 1 0.0 6 1 79 3 0.0 6 3 80 1 0.0 6 1 81 1 0.0 6 1 83 1 0.0 6 1 85 1 0.0 6 1 87 1 0.0 6 1 88 2 0.0 6 2 89 2 0.0 6 2 90 1 0.0 6 1 97 1 0.0 6 1 99 1 0.0 6 1 101 2 0.0 6 2 106 2 0.0 6 2 110 2 0.0 6 2 113 1 0.0 6 1 116 1 0.0 6 1 121 1 0.0 6 1 123 1 0.0 6 1 131 2 0.0 6 2 132 1 0.0 6 1 134 2 0.0 6 2 136 1 0.0 6 1 138 1 0.0 6 1 148 1 0.0 6 1 149 1 0.0 6 1 151 1 0.0 6 1 179 1 0.0 6 1 180 1 0.0 6 1 194 1 0.0 6 1 203 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_11_217_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_11_444_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_11_217_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_11_444_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.48 s (53 µs/read; 1.13 M reads/minute). === Summary === Total read pairs processed: 46,889 Read 1 with adapter: 46,889 (100.0%) Read 2 with adapter: 46,867 (100.0%) == Read fate breakdown == Pairs that were too short: 3 (0.0%) Pairs discarded as untrimmed: 22 (0.0%) Pairs written (passing filters): 46,864 (99.9%) Total basepairs processed: 23,861,268 bp Read 1: 13,491,080 bp Read 2: 10,370,188 bp Total written (filtered): 2,865,816 bp (12.0%) Read 1: 1,504,186 bp Read 2: 1,361,630 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 46800 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 732.6 0 0 1 4 2 183.2 1 1 1 6 1 11.4 1 0 0 1 14 2 0.0 4 2 16 1 0.0 4 1 17 1 0.0 5 1 19 1 0.0 5 1 20 11 0.0 6 11 21 3 0.0 6 3 22 4 0.0 6 4 23 3 0.0 6 3 24 1 0.0 6 1 25 4 0.0 6 4 26 1 0.0 6 1 27 4 0.0 6 4 28 7 0.0 6 7 30 1 0.0 6 1 31 3 0.0 6 2 0 0 1 33 1 0.0 6 1 35 1 0.0 6 1 37 1 0.0 6 0 1 38 1 0.0 6 1 39 1 0.0 6 1 40 3 0.0 6 3 41 1 0.0 6 1 42 6 0.0 6 5 1 43 3 0.0 6 3 44 2 0.0 6 2 45 3 0.0 6 3 46 1 0.0 6 1 47 2 0.0 6 2 48 2 0.0 6 2 49 1 0.0 6 0 1 50 3 0.0 6 3 53 5 0.0 6 5 54 2 0.0 6 2 55 1 0.0 6 1 56 4 0.0 6 4 57 5 0.0 6 4 0 0 1 59 1 0.0 6 1 60 1 0.0 6 0 1 61 2 0.0 6 2 62 3 0.0 6 3 63 2 0.0 6 2 64 3 0.0 6 2 0 1 65 4 0.0 6 3 1 66 1 0.0 6 1 67 1 0.0 6 1 69 2 0.0 6 2 74 1 0.0 6 1 76 4 0.0 6 3 1 77 2 0.0 6 2 78 4 0.0 6 4 79 2 0.0 6 2 80 2 0.0 6 2 82 1 0.0 6 1 84 1 0.0 6 1 85 2 0.0 6 2 86 6 0.0 6 6 87 2 0.0 6 2 89 8 0.0 6 8 90 1 0.0 6 1 91 3 0.0 6 2 1 92 1 0.0 6 1 93 3 0.0 6 3 94 1 0.0 6 1 96 3 0.0 6 3 97 4 0.0 6 4 98 7 0.0 6 6 1 99 3 0.0 6 3 100 7 0.0 6 6 1 101 8 0.0 6 8 102 3 0.0 6 2 1 103 3 0.0 6 3 104 8 0.0 6 8 105 6 0.0 6 6 106 4 0.0 6 3 1 107 5 0.0 6 4 1 108 11 0.0 6 11 109 4 0.0 6 4 110 7 0.0 6 7 111 4 0.0 6 4 112 5 0.0 6 4 1 113 5 0.0 6 5 114 5 0.0 6 5 115 5 0.0 6 5 116 8 0.0 6 8 117 6 0.0 6 6 118 5 0.0 6 5 119 7 0.0 6 7 120 2 0.0 6 2 121 10 0.0 6 9 1 122 13 0.0 6 10 3 123 7 0.0 6 7 124 8 0.0 6 6 2 125 8 0.0 6 7 1 126 8 0.0 6 8 127 3 0.0 6 3 128 10 0.0 6 10 129 5 0.0 6 5 130 8 0.0 6 8 131 3 0.0 6 3 132 11 0.0 6 10 1 133 7 0.0 6 7 134 14 0.0 6 13 0 1 135 15 0.0 6 15 136 15 0.0 6 14 1 137 18 0.0 6 17 1 138 19 0.0 6 19 139 15 0.0 6 15 140 31 0.0 6 31 141 43 0.0 6 42 1 142 78 0.0 6 78 143 79 0.0 6 79 144 91 0.0 6 91 145 82 0.0 6 82 146 90 0.0 6 90 147 95 0.0 6 95 148 111 0.0 6 111 149 85 0.0 6 85 150 131 0.0 6 129 2 151 75 0.0 6 72 3 152 74 0.0 6 72 2 153 65 0.0 6 65 154 54 0.0 6 54 155 54 0.0 6 53 1 156 50 0.0 6 48 2 157 54 0.0 6 53 1 158 54 0.0 6 54 159 59 0.0 6 58 1 160 52 0.0 6 52 161 50 0.0 6 50 162 36 0.0 6 35 1 163 44 0.0 6 42 1 1 164 59 0.0 6 58 1 165 46 0.0 6 45 1 166 26 0.0 6 26 167 28 0.0 6 28 168 51 0.0 6 51 169 62 0.0 6 61 1 170 49 0.0 6 49 171 54 0.0 6 54 172 26 0.0 6 26 173 55 0.0 6 54 0 1 174 55 0.0 6 53 1 1 175 29 0.0 6 29 176 33 0.0 6 32 1 177 37 0.0 6 37 178 51 0.0 6 49 2 179 35 0.0 6 34 1 180 41 0.0 6 41 181 25 0.0 6 25 182 25 0.0 6 24 1 183 58 0.0 6 57 1 184 46 0.0 6 46 185 52 0.0 6 52 186 52 0.0 6 51 1 187 40 0.0 6 40 188 57 0.0 6 56 1 189 42 0.0 6 42 190 36 0.0 6 34 2 191 76 0.0 6 76 192 61 0.0 6 61 193 80 0.0 6 76 3 1 194 109 0.0 6 108 1 195 74 0.0 6 74 196 87 0.0 6 87 197 103 0.0 6 101 2 198 89 0.0 6 88 1 199 67 0.0 6 65 2 200 66 0.0 6 66 201 64 0.0 6 63 1 202 71 0.0 6 70 1 203 94 0.0 6 91 3 204 93 0.0 6 90 3 205 108 0.0 6 104 4 206 87 0.0 6 83 4 207 103 0.0 6 100 3 208 135 0.0 6 134 1 209 190 0.0 6 187 3 210 157 0.0 6 150 7 211 290 0.0 6 279 11 212 165 0.0 6 164 1 213 161 0.0 6 155 6 214 319 0.0 6 317 2 215 95 0.0 6 93 2 216 273 0.0 6 267 5 0 0 1 217 141 0.0 6 136 4 0 1 218 141 0.0 6 137 4 219 115 0.0 6 112 3 220 131 0.0 6 128 2 1 221 84 0.0 6 81 2 1 222 210 0.0 6 209 1 223 71 0.0 6 68 2 0 0 1 224 148 0.0 6 146 2 225 133 0.0 6 124 9 226 391 0.0 6 385 6 227 271 0.0 6 260 11 228 205 0.0 6 198 7 229 228 0.0 6 223 5 230 227 0.0 6 220 7 231 191 0.0 6 187 4 232 161 0.0 6 158 3 233 143 0.0 6 140 3 234 114 0.0 6 113 1 235 83 0.0 6 81 2 236 110 0.0 6 108 2 237 131 0.0 6 129 2 238 95 0.0 6 94 0 1 239 176 0.0 6 173 3 240 405 0.0 6 396 9 241 153 0.0 6 151 2 242 172 0.0 6 170 2 243 147 0.0 6 144 2 0 1 244 106 0.0 6 96 8 1 1 245 152 0.0 6 147 5 246 140 0.0 6 134 6 247 234 0.0 6 230 4 248 148 0.0 6 147 1 249 199 0.0 6 192 7 250 312 0.0 6 303 9 251 249 0.0 6 235 14 252 610 0.0 6 601 8 1 253 240 0.0 6 235 5 254 259 0.0 6 251 8 255 250 0.0 6 240 9 1 256 361 0.0 6 353 7 1 257 460 0.0 6 449 10 1 258 424 0.0 6 409 14 1 259 661 0.0 6 648 13 260 415 0.0 6 398 17 261 495 0.0 6 486 8 1 262 321 0.0 6 298 23 263 616 0.0 6 609 7 264 274 0.0 6 269 5 265 272 0.0 6 269 3 266 373 0.0 6 364 8 1 267 503 0.0 6 487 16 268 263 0.0 6 253 9 1 269 532 0.0 6 520 11 1 270 273 0.0 6 269 4 271 300 0.0 6 296 4 272 382 0.0 6 377 5 273 337 0.0 6 329 8 274 388 0.0 6 375 13 275 670 0.0 6 651 19 276 1826 0.0 6 1790 35 1 277 1339 0.0 6 1309 28 2 278 1543 0.0 6 1418 122 2 1 279 19728 0.0 6 19400 319 7 1 1 280 30 0.0 6 27 2 0 1 281 12 0.0 6 11 0 1 282 2 0.0 6 0 1 0 1 285 4 0.0 6 4 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 89 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 4 183.2 1 0 4 63 3 0.0 6 3 65 1 0.0 6 1 73 1 0.0 6 1 74 1 0.0 6 1 76 3 0.0 6 3 77 2 0.0 6 2 78 5 0.0 6 5 79 3 0.0 6 3 80 2 0.0 6 2 81 1 0.0 6 1 83 1 0.0 6 1 84 2 0.0 6 2 85 2 0.0 6 2 86 2 0.0 6 2 87 2 0.0 6 2 89 1 0.0 6 1 91 1 0.0 6 1 93 4 0.0 6 4 94 1 0.0 6 1 95 4 0.0 6 4 96 3 0.0 6 3 100 1 0.0 6 1 102 1 0.0 6 1 109 2 0.0 6 2 110 1 0.0 6 1 112 1 0.0 6 1 115 1 0.0 6 1 116 1 0.0 6 1 120 1 0.0 6 1 121 1 0.0 6 1 123 1 0.0 6 1 124 2 0.0 6 2 131 1 0.0 6 1 134 2 0.0 6 2 135 1 0.0 6 1 138 1 0.0 6 1 143 2 0.0 6 2 145 1 0.0 6 1 146 1 0.0 6 1 159 1 0.0 6 1 161 1 0.0 6 1 163 1 0.0 6 1 177 1 0.0 6 1 180 1 0.0 6 1 182 1 0.0 6 1 185 1 0.0 6 1 189 1 0.0 6 1 190 1 0.0 6 1 198 1 0.0 6 1 199 1 0.0 6 1 200 1 0.0 6 1 201 1 0.0 6 1 225 1 0.0 6 1 226 1 0.0 6 1 244 1 0.0 6 1 248 1 0.0 6 1 250 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 46800 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 5 1 45.8 1 0 1 7 3 2.9 2 3 8 1 0.7 2 0 1 11 2 0.0 3 2 13 3 0.0 3 2 1 14 4 0.0 4 2 1 1 16 2 0.0 4 2 17 4 0.0 5 4 18 1 0.0 5 1 19 11 0.0 5 11 20 39 0.0 6 37 2 21 24 0.0 6 24 22 22 0.0 6 21 1 23 13 0.0 6 13 24 13 0.0 6 13 25 8 0.0 6 8 26 5 0.0 6 5 27 6 0.0 6 5 1 28 8 0.0 6 8 29 10 0.0 6 10 30 8 0.0 6 8 31 4 0.0 6 3 1 32 7 0.0 6 6 1 33 8 0.0 6 8 34 10 0.0 6 10 35 2 0.0 6 2 36 1 0.0 6 1 37 6 0.0 6 6 38 5 0.0 6 5 39 7 0.0 6 7 40 3 0.0 6 3 41 2 0.0 6 2 42 6 0.0 6 6 43 2 0.0 6 2 44 12 0.0 6 11 1 45 8 0.0 6 8 46 3 0.0 6 2 1 47 8 0.0 6 8 48 10 0.0 6 10 49 13 0.0 6 11 2 50 5 0.0 6 3 2 51 8 0.0 6 6 2 52 14 0.0 6 13 1 53 5 0.0 6 5 54 6 0.0 6 5 0 0 0 1 55 15 0.0 6 13 2 56 14 0.0 6 13 1 57 15 0.0 6 13 2 58 8 0.0 6 7 1 59 7 0.0 6 5 2 60 10 0.0 6 10 61 11 0.0 6 10 1 62 10 0.0 6 8 2 63 12 0.0 6 12 64 4 0.0 6 4 65 8 0.0 6 7 0 0 0 1 66 8 0.0 6 5 3 67 14 0.0 6 13 1 68 13 0.0 6 13 69 18 0.0 6 17 1 70 97 0.0 6 92 5 71 11 0.0 6 9 1 1 72 53 0.0 6 50 3 73 17 0.0 6 16 1 74 18 0.0 6 17 1 75 15 0.0 6 14 1 76 7 0.0 6 5 2 77 8 0.0 6 5 3 78 21 0.0 6 20 1 79 8 0.0 6 7 1 80 6 0.0 6 6 81 5 0.0 6 4 0 0 0 1 82 19 0.0 6 17 2 83 20 0.0 6 20 84 27 0.0 6 25 1 1 85 25 0.0 6 23 2 86 38 0.0 6 35 3 87 37 0.0 6 34 3 88 15 0.0 6 15 89 15 0.0 6 15 90 20 0.0 6 18 2 91 19 0.0 6 16 2 0 0 1 92 11 0.0 6 11 93 25 0.0 6 22 3 94 44 0.0 6 39 4 0 1 95 24 0.0 6 23 1 96 25 0.0 6 25 97 21 0.0 6 20 1 98 13 0.0 6 11 2 99 31 0.0 6 30 1 100 39 0.0 6 39 101 29 0.0 6 27 2 102 36 0.0 6 32 3 1 103 44 0.0 6 40 4 104 41 0.0 6 38 3 105 49 0.0 6 46 3 106 36 0.0 6 32 3 1 107 39 0.0 6 38 1 108 27 0.0 6 25 2 109 15 0.0 6 14 1 110 33 0.0 6 32 1 111 32 0.0 6 30 2 112 28 0.0 6 24 3 1 113 25 0.0 6 24 0 1 114 21 0.0 6 17 4 115 30 0.0 6 27 3 116 31 0.0 6 24 7 117 21 0.0 6 18 1 1 0 1 118 33 0.0 6 31 1 1 119 31 0.0 6 29 2 120 32 0.0 6 30 2 121 30 0.0 6 29 1 122 23 0.0 6 22 1 123 53 0.0 6 52 1 124 67 0.0 6 63 4 125 38 0.0 6 36 2 126 34 0.0 6 34 127 51 0.0 6 48 3 128 42 0.0 6 40 2 129 50 0.0 6 49 1 130 40 0.0 6 39 1 131 59 0.0 6 59 132 74 0.0 6 72 2 133 61 0.0 6 60 1 134 91 0.0 6 83 6 1 0 0 0 1 135 123 0.0 6 121 2 136 86 0.0 6 82 3 0 0 0 1 137 110 0.0 6 107 3 138 79 0.0 6 77 2 139 86 0.0 6 80 4 1 1 140 119 0.0 6 115 3 1 141 144 0.0 6 140 1 1 1 1 142 210 0.0 6 202 8 143 190 0.0 6 184 6 144 261 0.0 6 252 9 145 201 0.0 6 196 5 146 188 0.0 6 186 2 147 172 0.0 6 169 3 148 167 0.0 6 163 3 1 149 237 0.0 6 231 4 1 0 1 150 264 0.0 6 255 9 151 237 0.0 6 232 4 0 0 1 152 280 0.0 6 273 7 153 292 0.0 6 290 2 154 245 0.0 6 240 5 155 233 0.0 6 227 6 156 215 0.0 6 211 4 157 243 0.0 6 237 5 1 158 300 0.0 6 295 5 159 144 0.0 6 137 6 1 160 281 0.0 6 278 3 161 201 0.0 6 198 3 162 151 0.0 6 147 4 163 299 0.0 6 293 5 0 0 1 164 264 0.0 6 258 5 1 165 320 0.0 6 314 6 166 414 0.0 6 405 8 0 0 1 167 244 0.0 6 238 5 0 1 168 394 0.0 6 385 9 169 222 0.0 6 215 7 170 229 0.0 6 221 7 0 0 1 171 307 0.0 6 302 5 172 146 0.0 6 141 5 173 245 0.0 6 233 11 1 174 254 0.0 6 245 8 0 0 1 175 496 0.0 6 476 18 2 176 367 0.0 6 356 10 1 177 561 0.0 6 542 17 2 178 311 0.0 6 298 13 179 389 0.0 6 376 13 180 384 0.0 6 375 8 0 1 181 500 0.0 6 488 11 0 0 1 182 220 0.0 6 213 7 183 386 0.0 6 376 10 184 153 0.0 6 147 6 185 189 0.0 6 179 8 1 1 186 562 0.0 6 551 10 0 1 187 532 0.0 6 515 17 188 785 0.0 6 761 23 0 1 189 676 0.0 6 651 22 0 0 3 190 918 0.0 6 896 22 191 876 0.0 6 862 14 192 550 0.0 6 541 9 193 712 0.0 6 690 22 194 723 0.0 6 708 15 195 570 0.0 6 551 19 196 1426 0.0 6 1395 29 0 0 1 1 197 370 0.0 6 354 16 198 1093 0.0 6 1069 21 2 0 1 199 689 0.0 6 672 14 0 0 1 1 1 200 837 0.0 6 813 23 0 0 1 201 925 0.0 6 909 15 0 1 202 678 0.0 6 658 20 203 568 0.0 6 559 7 1 0 1 204 570 0.0 6 553 17 205 600 0.0 6 588 10 1 0 1 206 471 0.0 6 458 11 2 207 426 0.0 6 414 12 208 928 0.0 6 908 19 0 0 1 209 178 0.0 6 167 10 0 0 1 210 445 0.0 6 430 14 0 0 1 211 625 0.0 6 612 13 212 453 0.0 6 440 12 1 213 125 0.0 6 122 3 214 448 0.0 6 435 12 1 215 544 0.0 6 527 15 1 1 216 662 0.0 6 648 14 217 374 0.0 6 368 6 218 259 0.0 6 252 7 219 227 0.0 6 217 9 0 0 1 220 144 0.0 6 141 3 221 228 0.0 6 219 9 222 269 0.0 6 262 7 223 203 0.0 6 200 3 224 173 0.0 6 169 4 225 238 0.0 6 223 14 0 1 226 459 0.0 6 444 15 227 836 0.0 6 814 22 228 507 0.0 6 492 15 229 622 0.0 6 598 24 230 1136 0.0 6 1111 24 0 0 0 1 231 481 0.0 6 462 19 232 1319 0.0 6 1285 34 233 176 0.0 6 172 4 234 382 0.0 6 371 9 1 0 1 235 336 0.0 6 328 8 236 221 0.0 6 209 12 237 294 0.0 6 291 3 238 132 0.0 6 129 2 0 0 1 239 31 0.0 6 31 240 69 0.0 6 66 3 241 154 0.0 6 152 2 242 112 0.0 6 108 4 243 26 0.0 6 26 244 111 0.0 6 108 2 1 245 317 0.0 6 308 9 246 147 0.0 6 143 4 247 136 0.0 6 132 4 248 88 0.0 6 87 1 249 144 0.0 6 141 3 250 99 0.0 6 99 251 256 0.0 6 255 1 252 133 0.0 6 126 6 1 253 34 0.0 6 33 1 254 303 0.0 6 293 10 255 51 0.0 6 51 256 43 0.0 6 43 258 1 0.0 6 1 259 1 0.0 6 1 260 1 0.0 6 1 261 1 0.0 6 1 266 2 0.0 6 2 267 2 0.0 6 2 269 1 0.0 6 1 277 1 0.0 6 1 279 1 0.0 6 1 280 8 0.0 6 7 1 281 35 0.0 6 34 1 282 167 0.0 6 162 5 283 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 67 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 1 0.0 6 1 72 2 0.0 6 2 73 1 0.0 6 1 74 1 0.0 6 1 75 2 0.0 6 2 76 2 0.0 6 2 77 2 0.0 6 2 78 4 0.0 6 4 79 3 0.0 6 3 81 1 0.0 6 1 82 1 0.0 6 1 83 1 0.0 6 1 85 2 0.0 6 2 86 1 0.0 6 1 87 1 0.0 6 1 88 1 0.0 6 1 89 1 0.0 6 1 90 1 0.0 6 1 92 3 0.0 6 3 97 2 0.0 6 2 102 1 0.0 6 1 104 1 0.0 6 1 109 1 0.0 6 1 111 1 0.0 6 1 117 1 0.0 6 1 118 2 0.0 6 2 121 1 0.0 6 1 122 1 0.0 6 1 123 1 0.0 6 1 125 1 0.0 6 1 127 1 0.0 6 1 128 1 0.0 6 1 129 2 0.0 6 2 132 1 0.0 6 1 133 4 0.0 6 4 141 1 0.0 6 1 142 1 0.0 6 1 144 1 0.0 6 1 146 1 0.0 6 1 148 1 0.0 6 1 154 1 0.0 6 1 161 2 0.0 6 2 163 1 0.0 6 1 165 1 0.0 6 1 168 1 0.0 6 1 174 1 0.0 6 1 182 1 0.0 6 1 202 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_12_218_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_12_445_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_12_218_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_12_445_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.59 s (63 µs/read; 0.96 M reads/minute). === Summary === Total read pairs processed: 41,366 Read 1 with adapter: 41,366 (100.0%) Read 2 with adapter: 41,319 (99.9%) == Read fate breakdown == Pairs that were too short: 18 (0.0%) Pairs discarded as untrimmed: 47 (0.1%) Pairs written (passing filters): 41,301 (99.8%) Total basepairs processed: 17,442,744 bp Read 1: 9,748,562 bp Read 2: 7,694,182 bp Total written (filtered): 2,668,521 bp (15.3%) Read 1: 1,364,124 bp Read 2: 1,304,397 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 40509 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 2 161.6 1 1 1 7 4 2.5 2 3 0 1 9 2 0.2 2 1 1 12 1 0.0 3 1 15 3 0.0 4 3 17 3 0.0 5 3 19 3 0.0 5 2 1 20 49 0.0 6 48 1 21 21 0.0 6 21 22 23 0.0 6 22 1 23 19 0.0 6 19 24 11 0.0 6 11 25 7 0.0 6 7 26 17 0.0 6 17 27 6 0.0 6 6 28 1 0.0 6 1 29 8 0.0 6 8 30 4 0.0 6 4 31 3 0.0 6 3 32 2 0.0 6 2 33 7 0.0 6 7 34 3 0.0 6 2 1 35 1 0.0 6 1 36 1 0.0 6 1 37 4 0.0 6 4 38 5 0.0 6 5 39 7 0.0 6 7 40 6 0.0 6 6 41 3 0.0 6 3 42 9 0.0 6 9 43 2 0.0 6 2 44 1 0.0 6 1 45 3 0.0 6 3 46 3 0.0 6 3 47 4 0.0 6 4 48 4 0.0 6 4 49 5 0.0 6 5 50 2 0.0 6 2 51 7 0.0 6 6 1 52 3 0.0 6 3 53 3 0.0 6 3 54 3 0.0 6 3 55 1 0.0 6 1 56 1 0.0 6 1 57 5 0.0 6 5 58 4 0.0 6 4 59 3 0.0 6 3 61 1 0.0 6 1 62 2 0.0 6 2 63 3 0.0 6 2 1 64 4 0.0 6 4 65 3 0.0 6 2 1 66 2 0.0 6 2 67 2 0.0 6 2 68 1 0.0 6 1 69 5 0.0 6 5 70 8 0.0 6 8 71 6 0.0 6 6 72 6 0.0 6 6 73 2 0.0 6 2 74 7 0.0 6 7 75 8 0.0 6 8 76 6 0.0 6 6 77 2 0.0 6 2 78 3 0.0 6 3 79 6 0.0 6 6 80 6 0.0 6 6 81 4 0.0 6 4 82 6 0.0 6 5 0 0 1 83 6 0.0 6 5 1 84 14 0.0 6 14 85 4 0.0 6 4 86 5 0.0 6 5 87 3 0.0 6 3 88 4 0.0 6 4 89 12 0.0 6 11 1 90 7 0.0 6 6 1 91 5 0.0 6 5 92 5 0.0 6 4 1 93 4 0.0 6 4 94 6 0.0 6 5 1 95 4 0.0 6 4 96 11 0.0 6 10 1 97 5 0.0 6 5 98 7 0.0 6 7 99 4 0.0 6 4 100 9 0.0 6 9 101 9 0.0 6 9 102 13 0.0 6 13 103 4 0.0 6 3 1 104 13 0.0 6 13 105 15 0.0 6 15 106 14 0.0 6 13 0 0 1 107 13 0.0 6 13 108 12 0.0 6 11 0 1 109 15 0.0 6 15 110 12 0.0 6 12 111 17 0.0 6 17 112 11 0.0 6 11 113 17 0.0 6 17 114 12 0.0 6 12 115 11 0.0 6 11 116 19 0.0 6 19 117 10 0.0 6 10 118 18 0.0 6 18 119 17 0.0 6 17 120 8 0.0 6 8 121 11 0.0 6 11 122 16 0.0 6 16 123 16 0.0 6 16 124 19 0.0 6 19 125 16 0.0 6 16 126 19 0.0 6 19 127 18 0.0 6 18 128 25 0.0 6 21 3 0 0 0 1 129 28 0.0 6 25 2 0 0 1 130 16 0.0 6 15 0 0 0 0 0 1 131 22 0.0 6 22 132 16 0.0 6 16 133 19 0.0 6 19 134 25 0.0 6 24 0 0 1 135 30 0.0 6 30 136 25 0.0 6 25 137 25 0.0 6 24 0 1 138 31 0.0 6 31 139 32 0.0 6 32 140 33 0.0 6 32 1 141 42 0.0 6 41 1 142 136 0.0 6 136 143 290 0.0 6 290 144 363 0.0 6 363 145 296 0.0 6 296 146 335 0.0 6 333 1 0 0 0 1 147 390 0.0 6 389 1 148 343 0.0 6 342 1 149 454 0.0 6 454 150 405 0.0 6 405 151 370 0.0 6 369 1 152 416 0.0 6 416 153 271 0.0 6 271 154 261 0.0 6 259 2 155 243 0.0 6 243 156 234 0.0 6 234 157 215 0.0 6 213 2 158 229 0.0 6 229 159 258 0.0 6 258 160 257 0.0 6 257 161 331 0.0 6 329 2 162 303 0.0 6 301 2 163 302 0.0 6 302 164 258 0.0 6 256 1 0 1 165 227 0.0 6 227 166 195 0.0 6 194 1 167 259 0.0 6 259 168 197 0.0 6 197 169 192 0.0 6 191 1 170 232 0.0 6 230 1 0 0 0 0 1 171 200 0.0 6 200 172 253 0.0 6 252 1 173 203 0.0 6 202 1 174 193 0.0 6 193 175 225 0.0 6 223 1 0 0 0 0 1 176 207 0.0 6 206 1 177 179 0.0 6 178 1 178 172 0.0 6 172 179 208 0.0 6 207 1 180 553 0.0 6 552 1 181 248 0.0 6 246 2 182 218 0.0 6 216 2 183 235 0.0 6 235 184 223 0.0 6 222 0 0 0 0 1 185 225 0.0 6 224 0 1 186 236 0.0 6 235 1 187 249 0.0 6 247 2 188 224 0.0 6 223 1 189 221 0.0 6 221 190 196 0.0 6 196 191 223 0.0 6 223 192 263 0.0 6 263 193 252 0.0 6 249 3 194 284 0.0 6 283 1 195 407 0.0 6 407 196 402 0.0 6 397 5 197 426 0.0 6 421 5 198 496 0.0 6 494 2 199 354 0.0 6 349 4 1 200 290 0.0 6 286 3 0 0 0 1 201 386 0.0 6 385 1 202 315 0.0 6 313 2 203 321 0.0 6 320 1 204 469 0.0 6 466 3 205 347 0.0 6 347 206 494 0.0 6 491 3 207 401 0.0 6 397 2 1 0 0 0 1 208 375 0.0 6 373 2 209 348 0.0 6 346 2 210 373 0.0 6 371 2 211 450 0.0 6 442 7 1 212 411 0.0 6 411 213 476 0.0 6 473 3 214 484 0.0 6 481 3 215 567 0.0 6 565 0 1 0 0 0 1 216 482 0.0 6 478 4 217 461 0.0 6 459 2 218 469 0.0 6 469 219 428 0.0 6 424 4 220 340 0.0 6 339 1 221 373 0.0 6 371 1 1 222 376 0.0 6 376 223 371 0.0 6 371 224 393 0.0 6 392 1 225 469 0.0 6 467 2 226 440 0.0 6 437 2 1 227 369 0.0 6 368 1 228 334 0.0 6 334 229 350 0.0 6 350 230 361 0.0 6 358 3 231 364 0.0 6 364 232 329 0.0 6 327 2 233 383 0.0 6 379 2 1 1 234 353 0.0 6 351 2 235 281 0.0 6 279 1 0 1 236 291 0.0 6 290 0 0 0 0 0 1 237 330 0.0 6 327 3 238 257 0.0 6 257 239 307 0.0 6 307 240 329 0.0 6 326 3 241 268 0.0 6 266 2 242 282 0.0 6 281 1 243 270 0.0 6 268 2 244 249 0.0 6 249 245 206 0.0 6 206 246 198 0.0 6 197 1 247 182 0.0 6 182 248 193 0.0 6 191 2 249 200 0.0 6 199 1 250 180 0.0 6 179 1 251 172 0.0 6 171 1 252 184 0.0 6 184 253 165 0.0 6 164 1 254 160 0.0 6 160 255 162 0.0 6 161 1 256 149 0.0 6 146 2 0 1 257 152 0.0 6 152 258 149 0.0 6 147 1 0 0 0 1 259 161 0.0 6 161 260 136 0.0 6 135 1 261 114 0.0 6 114 262 123 0.0 6 123 263 95 0.0 6 95 264 121 0.0 6 120 1 265 98 0.0 6 98 266 116 0.0 6 116 267 126 0.0 6 126 268 110 0.0 6 110 269 107 0.0 6 107 270 100 0.0 6 100 271 81 0.0 6 81 272 77 0.0 6 77 273 86 0.0 6 86 274 110 0.0 6 109 1 275 138 0.0 6 136 2 276 142 0.0 6 142 277 198 0.0 6 198 278 604 0.0 6 602 1 1 279 1114 0.0 6 1110 4 280 4 0.0 6 4 281 1 0.0 6 0 1 284 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 857 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 4 7 161.6 1 0 7 63 53 0.0 6 53 70 1 0.0 6 1 72 6 0.0 6 6 73 6 0.0 6 6 74 9 0.0 6 9 75 8 0.0 6 8 76 10 0.0 6 10 77 15 0.0 6 15 78 9 0.0 6 9 79 14 0.0 6 14 80 6 0.0 6 6 81 7 0.0 6 7 82 14 0.0 6 14 83 5 0.0 6 5 84 4 0.0 6 4 85 2 0.0 6 2 86 5 0.0 6 5 87 2 0.0 6 2 88 7 0.0 6 7 89 11 0.0 6 11 90 8 0.0 6 8 91 9 0.0 6 9 92 5 0.0 6 5 93 7 0.0 6 7 94 4 0.0 6 4 95 6 0.0 6 6 96 5 0.0 6 5 97 2 0.0 6 2 98 3 0.0 6 3 99 4 0.0 6 4 100 5 0.0 6 5 101 6 0.0 6 6 102 6 0.0 6 6 103 5 0.0 6 5 104 2 0.0 6 2 105 3 0.0 6 3 106 7 0.0 6 7 107 6 0.0 6 6 108 5 0.0 6 5 109 6 0.0 6 6 110 9 0.0 6 9 111 6 0.0 6 6 112 2 0.0 6 2 113 5 0.0 6 4 0 0 0 0 0 1 114 4 0.0 6 4 115 1 0.0 6 1 116 4 0.0 6 4 117 3 0.0 6 3 118 3 0.0 6 3 119 1 0.0 6 1 120 2 0.0 6 2 121 4 0.0 6 4 122 2 0.0 6 2 123 1 0.0 6 1 124 5 0.0 6 5 125 10 0.0 6 10 126 2 0.0 6 2 127 5 0.0 6 5 128 6 0.0 6 6 129 4 0.0 6 4 130 4 0.0 6 4 131 2 0.0 6 2 132 2 0.0 6 2 133 3 0.0 6 3 134 7 0.0 6 7 135 8 0.0 6 8 136 4 0.0 6 4 137 5 0.0 6 5 138 5 0.0 6 5 139 5 0.0 6 5 140 5 0.0 6 5 141 4 0.0 6 4 142 5 0.0 6 5 143 3 0.0 6 3 144 4 0.0 6 4 145 2 0.0 6 2 146 2 0.0 6 2 147 3 0.0 6 3 148 5 0.0 6 5 149 3 0.0 6 3 150 2 0.0 6 2 151 4 0.0 6 4 152 5 0.0 6 5 153 4 0.0 6 4 154 2 0.0 6 2 155 4 0.0 6 4 156 3 0.0 6 3 157 4 0.0 6 4 158 6 0.0 6 6 159 6 0.0 6 6 160 2 0.0 6 2 161 3 0.0 6 3 162 2 0.0 6 2 163 4 0.0 6 4 164 5 0.0 6 5 165 3 0.0 6 3 166 7 0.0 6 7 167 6 0.0 6 6 168 5 0.0 6 5 169 4 0.0 6 4 170 5 0.0 6 5 171 3 0.0 6 3 172 5 0.0 6 5 173 6 0.0 6 6 174 4 0.0 6 4 175 3 0.0 6 3 176 5 0.0 6 5 177 2 0.0 6 2 178 3 0.0 6 3 179 5 0.0 6 5 180 5 0.0 6 5 181 5 0.0 6 5 182 3 0.0 6 3 183 3 0.0 6 2 1 186 4 0.0 6 4 187 3 0.0 6 3 188 8 0.0 6 8 189 6 0.0 6 6 190 4 0.0 6 4 191 6 0.0 6 6 192 6 0.0 6 6 193 4 0.0 6 4 194 3 0.0 6 3 195 2 0.0 6 2 196 5 0.0 6 5 197 3 0.0 6 3 198 3 0.0 6 3 199 5 0.0 6 5 200 3 0.0 6 3 201 4 0.0 6 4 202 6 0.0 6 6 203 8 0.0 6 8 204 3 0.0 6 3 205 3 0.0 6 3 206 2 0.0 6 2 207 2 0.0 6 2 208 4 0.0 6 4 209 2 0.0 6 2 210 3 0.0 6 3 211 6 0.0 6 6 212 7 0.0 6 7 213 5 0.0 6 5 214 6 0.0 6 6 215 3 0.0 6 3 216 3 0.0 6 3 217 1 0.0 6 1 218 3 0.0 6 3 219 4 0.0 6 4 220 3 0.0 6 3 221 1 0.0 6 1 222 3 0.0 6 3 223 2 0.0 6 2 224 1 0.0 6 1 225 4 0.0 6 4 226 7 0.0 6 7 227 2 0.0 6 2 228 8 0.0 6 8 229 6 0.0 6 6 230 3 0.0 6 3 231 3 0.0 6 3 232 3 0.0 6 3 233 2 0.0 6 2 234 1 0.0 6 1 236 3 0.0 6 3 237 3 0.0 6 3 238 2 0.0 6 2 239 2 0.0 6 2 240 2 0.0 6 2 241 3 0.0 6 3 243 1 0.0 6 1 245 3 0.0 6 3 246 5 0.0 6 5 247 2 0.0 6 2 248 2 0.0 6 2 249 1 0.0 6 1 250 3 0.0 6 3 252 1 0.0 6 1 253 2 0.0 6 2 254 4 0.0 6 4 255 1 0.0 6 1 260 2 0.0 6 2 261 3 0.0 6 3 263 2 0.0 6 2 265 3 0.0 6 3 266 2 0.0 6 2 268 1 0.0 6 1 269 1 0.0 6 1 270 1 0.0 6 1 271 1 0.0 6 1 274 1 0.0 6 1 279 2 0.0 6 2 280 1 0.0 6 1 281 1 0.0 6 1 283 1 0.0 6 1 285 1 0.0 6 1 286 1 0.0 6 1 295 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 40581 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 161.6 1 1 6 1 10.1 1 1 11 3 0.0 3 2 1 12 5 0.0 3 5 13 8 0.0 3 7 1 14 9 0.0 4 8 1 15 1 0.0 4 1 16 3 0.0 4 3 17 24 0.0 5 24 18 13 0.0 5 12 1 19 16 0.0 5 12 4 20 49 0.0 6 49 21 23 0.0 6 23 22 27 0.0 6 25 2 23 15 0.0 6 15 24 8 0.0 6 7 1 25 12 0.0 6 12 26 19 0.0 6 18 1 27 8 0.0 6 6 2 28 10 0.0 6 9 0 1 29 14 0.0 6 12 2 30 1 0.0 6 1 31 9 0.0 6 9 32 10 0.0 6 9 1 33 7 0.0 6 6 0 0 1 34 10 0.0 6 7 3 35 11 0.0 6 10 1 36 4 0.0 6 3 1 37 5 0.0 6 5 38 6 0.0 6 4 2 39 2 0.0 6 2 40 8 0.0 6 8 41 11 0.0 6 11 42 4 0.0 6 4 43 9 0.0 6 9 44 9 0.0 6 9 45 9 0.0 6 9 46 12 0.0 6 10 2 47 11 0.0 6 11 48 9 0.0 6 7 2 49 14 0.0 6 14 50 8 0.0 6 6 2 51 10 0.0 6 8 1 0 0 0 0 1 52 18 0.0 6 17 0 1 53 17 0.0 6 15 2 54 10 0.0 6 10 55 10 0.0 6 9 1 56 10 0.0 6 10 57 19 0.0 6 18 1 58 18 0.0 6 17 1 59 18 0.0 6 17 0 1 60 18 0.0 6 15 3 61 12 0.0 6 12 62 17 0.0 6 16 1 63 14 0.0 6 11 3 64 24 0.0 6 22 2 65 19 0.0 6 17 1 0 0 0 0 1 66 16 0.0 6 14 2 67 33 0.0 6 30 2 1 68 87 0.0 6 87 69 34 0.0 6 31 3 70 40 0.0 6 39 1 71 28 0.0 6 22 4 2 72 33 0.0 6 32 1 73 24 0.0 6 24 74 22 0.0 6 21 1 75 32 0.0 6 31 0 0 0 1 76 19 0.0 6 17 2 77 26 0.0 6 25 1 78 27 0.0 6 25 1 0 0 1 79 29 0.0 6 28 0 0 0 0 0 1 80 33 0.0 6 31 2 81 34 0.0 6 33 1 82 33 0.0 6 32 0 0 0 1 83 35 0.0 6 35 84 45 0.0 6 44 1 85 31 0.0 6 30 1 86 29 0.0 6 27 2 87 23 0.0 6 23 88 27 0.0 6 26 1 89 33 0.0 6 32 1 90 47 0.0 6 46 1 91 52 0.0 6 49 1 2 92 54 0.0 6 51 1 2 93 42 0.0 6 42 94 50 0.0 6 48 2 95 46 0.0 6 45 1 96 46 0.0 6 46 97 60 0.0 6 59 0 1 98 50 0.0 6 48 1 0 0 0 0 1 99 52 0.0 6 52 100 56 0.0 6 56 101 55 0.0 6 50 4 0 1 102 58 0.0 6 56 2 103 60 0.0 6 59 1 104 74 0.0 6 71 2 1 105 115 0.0 6 111 4 106 80 0.0 6 78 2 107 59 0.0 6 58 1 108 73 0.0 6 71 2 109 71 0.0 6 68 2 1 110 79 0.0 6 78 1 111 80 0.0 6 80 112 114 0.0 6 111 2 0 1 113 126 0.0 6 123 2 1 114 90 0.0 6 90 115 106 0.0 6 105 1 116 113 0.0 6 108 5 117 80 0.0 6 78 2 118 134 0.0 6 131 3 119 120 0.0 6 115 5 120 123 0.0 6 122 1 121 118 0.0 6 115 3 122 115 0.0 6 113 2 123 119 0.0 6 117 2 124 159 0.0 6 154 4 1 125 165 0.0 6 164 1 126 163 0.0 6 160 3 127 200 0.0 6 196 3 1 128 199 0.0 6 196 2 0 0 1 129 228 0.0 6 224 4 130 225 0.0 6 216 9 131 257 0.0 6 255 1 0 0 0 1 132 251 0.0 6 248 3 133 259 0.0 6 253 5 0 1 134 273 0.0 6 263 8 0 0 0 0 2 135 323 0.0 6 318 2 0 1 1 1 136 346 0.0 6 342 4 137 246 0.0 6 245 1 138 323 0.0 6 316 4 0 1 0 0 2 139 278 0.0 6 271 4 1 0 1 0 1 140 456 0.0 6 453 2 0 1 141 552 0.0 6 545 4 1 0 0 0 2 142 652 0.0 6 645 5 0 0 0 1 1 143 612 0.0 6 601 8 1 1 0 0 1 144 569 0.0 6 564 5 145 702 0.0 6 696 4 1 0 0 0 1 146 702 0.0 6 697 4 0 0 1 147 786 0.0 6 776 7 2 0 1 148 766 0.0 6 758 7 1 149 772 0.0 6 764 7 1 150 893 0.0 6 888 4 0 0 1 151 1045 0.0 6 1034 10 0 0 0 0 1 152 802 0.0 6 791 8 0 1 1 0 1 153 871 0.0 6 854 13 1 0 0 1 2 154 900 0.0 6 891 5 2 0 0 1 1 155 701 0.0 6 690 8 1 2 156 713 0.0 6 702 10 0 0 1 157 715 0.0 6 707 6 1 0 0 1 158 780 0.0 6 769 8 0 1 1 1 159 784 0.0 6 769 11 0 1 1 2 160 693 0.0 6 686 6 0 1 161 638 0.0 6 628 9 0 1 162 552 0.0 6 542 6 1 0 1 1 1 163 513 0.0 6 510 3 164 522 0.0 6 516 6 165 467 0.0 6 461 6 166 474 0.0 6 469 4 0 1 167 585 0.0 6 580 4 0 0 0 0 1 168 511 0.0 6 501 9 0 0 0 1 169 603 0.0 6 596 7 170 524 0.0 6 517 6 0 1 171 487 0.0 6 479 8 172 588 0.0 6 584 3 0 0 0 1 173 544 0.0 6 539 4 0 0 0 1 174 563 0.0 6 558 5 175 483 0.0 6 478 4 0 0 0 0 1 176 505 0.0 6 502 3 177 506 0.0 6 502 1 0 1 1 0 1 178 488 0.0 6 487 1 179 412 0.0 6 404 6 0 0 1 1 180 328 0.0 6 328 181 327 0.0 6 325 2 182 271 0.0 6 268 2 0 1 183 297 0.0 6 293 3 0 0 1 184 379 0.0 6 375 3 0 0 0 1 185 497 0.0 6 494 3 186 445 0.0 6 444 1 187 299 0.0 6 299 188 286 0.0 6 284 1 1 189 266 0.0 6 263 2 1 190 272 0.0 6 269 3 191 238 0.0 6 237 1 192 247 0.0 6 245 2 193 202 0.0 6 201 1 194 193 0.0 6 192 0 0 0 0 1 195 163 0.0 6 162 1 196 146 0.0 6 143 2 0 1 197 193 0.0 6 188 3 0 1 0 0 1 198 159 0.0 6 158 1 199 156 0.0 6 152 2 0 1 0 0 1 200 140 0.0 6 139 1 201 136 0.0 6 136 202 111 0.0 6 111 203 109 0.0 6 108 1 204 86 0.0 6 85 0 0 0 1 205 86 0.0 6 86 206 89 0.0 6 86 3 207 76 0.0 6 76 208 71 0.0 6 69 1 0 0 0 0 1 209 76 0.0 6 75 1 210 62 0.0 6 61 1 211 49 0.0 6 49 212 52 0.0 6 52 213 41 0.0 6 41 214 41 0.0 6 41 215 62 0.0 6 62 216 45 0.0 6 44 1 217 36 0.0 6 36 218 39 0.0 6 38 1 219 25 0.0 6 25 220 24 0.0 6 23 1 221 17 0.0 6 17 222 20 0.0 6 20 223 22 0.0 6 22 224 21 0.0 6 21 225 15 0.0 6 14 0 1 226 17 0.0 6 17 227 6 0.0 6 6 228 12 0.0 6 12 229 16 0.0 6 16 230 13 0.0 6 13 231 8 0.0 6 8 232 5 0.0 6 5 233 6 0.0 6 6 234 4 0.0 6 4 235 6 0.0 6 6 236 5 0.0 6 5 237 5 0.0 6 4 0 0 0 1 238 1 0.0 6 1 239 1 0.0 6 1 240 3 0.0 6 3 241 1 0.0 6 1 242 3 0.0 6 3 244 2 0.0 6 2 245 3 0.0 6 3 248 4 0.0 6 4 249 1 0.0 6 1 279 6 0.0 6 6 280 49 0.0 6 49 281 1 0.0 6 0 0 0 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 738 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 52 3 0.0 6 3 55 2 0.0 6 2 59 1 0.0 6 1 61 2 0.0 6 2 64 1 0.0 6 1 71 5 0.0 6 5 72 7 0.0 6 7 73 13 0.0 6 13 74 9 0.0 6 9 75 15 0.0 6 15 76 11 0.0 6 11 77 8 0.0 6 8 78 20 0.0 6 20 79 13 0.0 6 13 80 9 0.0 6 9 81 10 0.0 6 10 82 16 0.0 6 16 83 10 0.0 6 10 84 4 0.0 6 4 85 10 0.0 6 10 86 8 0.0 6 8 87 4 0.0 6 4 88 9 0.0 6 9 89 8 0.0 6 8 90 11 0.0 6 10 0 0 0 1 91 9 0.0 6 9 92 8 0.0 6 8 93 7 0.0 6 6 1 94 6 0.0 6 5 0 0 1 95 6 0.0 6 6 96 7 0.0 6 7 97 5 0.0 6 5 98 4 0.0 6 4 99 2 0.0 6 2 100 4 0.0 6 4 101 4 0.0 6 4 102 2 0.0 6 2 103 7 0.0 6 7 104 6 0.0 6 6 105 5 0.0 6 5 106 1 0.0 6 1 107 7 0.0 6 7 108 4 0.0 6 4 109 17 0.0 6 17 110 3 0.0 6 3 111 4 0.0 6 4 112 3 0.0 6 3 113 2 0.0 6 2 114 7 0.0 6 7 115 11 0.0 6 10 1 116 6 0.0 6 6 117 6 0.0 6 6 118 4 0.0 6 4 119 6 0.0 6 6 120 4 0.0 6 4 121 2 0.0 6 2 122 4 0.0 6 4 123 4 0.0 6 4 124 4 0.0 6 4 125 2 0.0 6 1 1 126 4 0.0 6 4 127 8 0.0 6 8 128 3 0.0 6 3 129 2 0.0 6 2 130 4 0.0 6 4 131 2 0.0 6 2 132 8 0.0 6 8 133 10 0.0 6 10 134 3 0.0 6 3 135 3 0.0 6 3 136 5 0.0 6 5 137 5 0.0 6 5 138 2 0.0 6 2 139 4 0.0 6 4 140 4 0.0 6 4 141 3 0.0 6 3 142 3 0.0 6 3 143 2 0.0 6 2 144 13 0.0 6 13 145 4 0.0 6 4 146 4 0.0 6 4 147 5 0.0 6 5 148 5 0.0 6 5 149 2 0.0 6 2 150 4 0.0 6 4 151 4 0.0 6 4 152 3 0.0 6 3 153 3 0.0 6 3 154 2 0.0 6 2 155 4 0.0 6 4 156 4 0.0 6 4 157 6 0.0 6 6 158 2 0.0 6 2 159 5 0.0 6 5 160 3 0.0 6 3 161 5 0.0 6 5 162 7 0.0 6 7 163 5 0.0 6 5 164 4 0.0 6 4 165 3 0.0 6 3 166 10 0.0 6 10 167 1 0.0 6 1 168 6 0.0 6 6 169 7 0.0 6 7 170 3 0.0 6 3 171 5 0.0 6 5 172 3 0.0 6 3 173 6 0.0 6 6 174 4 0.0 6 4 175 7 0.0 6 7 176 2 0.0 6 2 177 3 0.0 6 3 178 2 0.0 6 2 179 3 0.0 6 3 180 2 0.0 6 2 181 7 0.0 6 6 0 0 0 1 182 1 0.0 6 1 183 3 0.0 6 3 184 5 0.0 6 5 186 5 0.0 6 5 187 3 0.0 6 3 188 7 0.0 6 7 189 3 0.0 6 3 190 5 0.0 6 5 191 2 0.0 6 2 192 6 0.0 6 6 193 5 0.0 6 5 194 3 0.0 6 3 195 2 0.0 6 1 0 0 1 196 3 0.0 6 3 197 4 0.0 6 4 198 4 0.0 6 4 199 2 0.0 6 2 201 6 0.0 6 6 202 5 0.0 6 5 203 1 0.0 6 1 204 5 0.0 6 5 205 1 0.0 6 1 207 1 0.0 6 1 208 3 0.0 6 3 209 2 0.0 6 2 210 2 0.0 6 2 211 2 0.0 6 2 212 2 0.0 6 2 213 3 0.0 6 3 214 1 0.0 6 1 220 1 0.0 6 1 227 1 0.0 6 1 229 1 0.0 6 1 240 1 0.0 6 1 241 1 0.0 6 1 258 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_13_219_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_13_446_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_13_219_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_13_446_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.85 s (845092 µs/read; 0.00 M reads/minute). === Summary === Total read pairs processed: 1 Read 1 with adapter: 1 (100.0%) Read 2 with adapter: 1 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 0 (0.0%) Pairs written (passing filters): 1 (100.0%) Total basepairs processed: 477 bp Read 1: 307 bp Read 2: 170 bp Total written (filtered): 63 bp (13.2%) Read 1: 33 bp Read 2: 30 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 1 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 274 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 0 times === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 1 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 140 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 0 times This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_07_220_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_07_447_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_07_220_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_07_447_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 4.14 s (50 µs/read; 1.21 M reads/minute). === Summary === Total read pairs processed: 83,635 Read 1 with adapter: 83,631 (100.0%) Read 2 with adapter: 83,541 (99.9%) == Read fate breakdown == Pairs that were too short: 4 (0.0%) Pairs discarded as untrimmed: 98 (0.1%) Pairs written (passing filters): 83,533 (99.9%) Total basepairs processed: 40,744,411 bp Read 1: 23,420,885 bp Read 2: 17,323,526 bp Total written (filtered): 5,449,139 bp (13.4%) Read 1: 2,772,947 bp Read 2: 2,676,192 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 83203 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1306.8 0 1 1 4 2 326.7 1 1 1 7 1 5.1 2 0 0 1 8 1 1.3 2 1 9 3 0.3 2 3 10 1 0.1 3 1 14 2 0.0 4 2 17 1 0.0 5 0 1 20 22 0.0 6 20 2 21 13 0.0 6 11 2 22 5 0.0 6 5 23 7 0.0 6 7 24 3 0.0 6 2 1 25 5 0.0 6 4 1 26 6 0.0 6 6 27 3 0.0 6 3 28 3 0.0 6 3 30 2 0.0 6 2 31 4 0.0 6 4 32 3 0.0 6 1 2 33 6 0.0 6 6 34 1 0.0 6 1 35 1 0.0 6 1 36 2 0.0 6 1 1 37 1 0.0 6 1 38 2 0.0 6 2 39 2 0.0 6 1 1 40 5 0.0 6 5 41 3 0.0 6 1 1 0 0 0 1 42 3 0.0 6 2 1 43 2 0.0 6 2 44 2 0.0 6 2 45 8 0.0 6 8 46 5 0.0 6 5 48 5 0.0 6 4 0 1 49 2 0.0 6 2 50 8 0.0 6 7 1 51 1 0.0 6 0 1 52 1 0.0 6 0 1 53 3 0.0 6 3 54 3 0.0 6 3 55 5 0.0 6 5 56 8 0.0 6 8 57 8 0.0 6 7 1 58 3 0.0 6 2 1 59 1 0.0 6 0 1 60 2 0.0 6 1 0 1 61 1 0.0 6 1 62 4 0.0 6 4 63 6 0.0 6 6 64 8 0.0 6 6 2 65 5 0.0 6 5 66 2 0.0 6 2 67 5 0.0 6 5 68 4 0.0 6 4 69 5 0.0 6 5 70 2 0.0 6 2 71 4 0.0 6 3 1 72 1 0.0 6 1 73 2 0.0 6 1 1 74 3 0.0 6 3 75 4 0.0 6 4 77 2 0.0 6 2 78 3 0.0 6 3 80 2 0.0 6 2 81 3 0.0 6 3 82 1 0.0 6 1 83 1 0.0 6 0 1 84 2 0.0 6 1 1 85 4 0.0 6 4 86 12 0.0 6 12 87 7 0.0 6 7 88 1 0.0 6 1 89 5 0.0 6 3 2 90 7 0.0 6 5 2 91 5 0.0 6 4 1 92 1 0.0 6 1 93 2 0.0 6 2 94 2 0.0 6 2 95 3 0.0 6 2 1 96 1 0.0 6 1 97 6 0.0 6 6 98 3 0.0 6 2 1 99 6 0.0 6 5 1 100 14 0.0 6 13 0 0 1 101 10 0.0 6 9 1 102 37 0.0 6 35 2 103 12 0.0 6 10 1 1 104 7 0.0 6 3 3 0 1 105 12 0.0 6 11 1 106 9 0.0 6 9 107 15 0.0 6 15 108 17 0.0 6 17 109 6 0.0 6 6 110 7 0.0 6 6 1 111 22 0.0 6 20 1 1 112 15 0.0 6 15 113 12 0.0 6 10 2 114 20 0.0 6 20 115 9 0.0 6 7 1 1 116 11 0.0 6 11 117 9 0.0 6 8 1 118 6 0.0 6 6 119 6 0.0 6 5 1 120 7 0.0 6 6 0 0 1 121 17 0.0 6 16 1 122 23 0.0 6 20 3 123 15 0.0 6 13 2 124 8 0.0 6 7 0 0 1 125 11 0.0 6 11 126 7 0.0 6 6 1 127 7 0.0 6 6 1 128 21 0.0 6 20 1 129 11 0.0 6 10 1 130 13 0.0 6 11 2 131 14 0.0 6 14 132 15 0.0 6 15 133 14 0.0 6 14 134 16 0.0 6 15 1 135 15 0.0 6 12 3 136 10 0.0 6 10 137 21 0.0 6 20 1 138 31 0.0 6 30 1 139 16 0.0 6 16 140 24 0.0 6 23 1 141 38 0.0 6 33 5 142 27 0.0 6 25 2 143 143 0.0 6 142 0 0 1 144 87 0.0 6 87 145 125 0.0 6 125 146 61 0.0 6 59 2 147 49 0.0 6 47 2 148 70 0.0 6 69 1 149 133 0.0 6 133 150 66 0.0 6 66 151 69 0.0 6 68 1 152 34 0.0 6 34 153 37 0.0 6 34 3 154 39 0.0 6 38 1 155 83 0.0 6 80 2 0 1 156 38 0.0 6 36 2 157 121 0.0 6 119 2 158 70 0.0 6 69 1 159 43 0.0 6 38 5 160 66 0.0 6 66 161 52 0.0 6 51 1 162 45 0.0 6 40 4 0 1 163 122 0.0 6 116 5 0 0 0 0 1 164 65 0.0 6 63 1 1 165 48 0.0 6 44 4 166 33 0.0 6 32 1 167 79 0.0 6 73 6 168 81 0.0 6 77 4 169 56 0.0 6 53 3 170 81 0.0 6 77 4 171 68 0.0 6 63 5 172 62 0.0 6 56 6 173 72 0.0 6 69 3 174 70 0.0 6 66 4 175 130 0.0 6 119 10 0 1 176 114 0.0 6 105 9 177 108 0.0 6 101 7 178 99 0.0 6 97 2 179 70 0.0 6 68 2 180 83 0.0 6 80 3 181 118 0.0 6 117 1 182 137 0.0 6 134 3 183 132 0.0 6 129 3 184 155 0.0 6 146 7 2 185 175 0.0 6 160 15 186 235 0.0 6 222 11 1 0 0 0 1 187 102 0.0 6 96 6 188 195 0.0 6 188 6 1 189 251 0.0 6 235 16 190 164 0.0 6 152 12 191 283 0.0 6 272 11 192 296 0.0 6 283 11 2 193 335 0.0 6 322 12 1 194 350 0.0 6 331 19 195 451 0.0 6 426 24 1 196 606 0.0 6 576 28 2 197 473 0.0 6 447 26 198 353 0.0 6 329 24 199 331 0.0 6 319 12 200 279 0.0 6 268 10 1 201 231 0.0 6 212 18 1 202 323 0.0 6 307 16 203 306 0.0 6 288 18 204 332 0.0 6 313 17 2 205 435 0.0 6 415 17 3 206 323 0.0 6 300 23 207 447 0.0 6 415 32 208 341 0.0 6 329 11 1 209 303 0.0 6 286 17 210 372 0.0 6 348 24 211 290 0.0 6 283 7 212 272 0.0 6 259 13 213 336 0.0 6 318 18 214 287 0.0 6 275 12 215 188 0.0 6 180 7 1 216 291 0.0 6 279 10 2 217 416 0.0 6 396 19 1 218 265 0.0 6 246 15 4 219 423 0.0 6 402 18 2 0 1 220 566 0.0 6 526 37 2 1 221 277 0.0 6 261 15 0 1 222 395 0.0 6 368 27 223 705 0.0 6 670 34 1 224 370 0.0 6 348 22 225 1313 0.0 6 1243 69 1 226 693 0.0 6 663 30 227 479 0.0 6 444 35 228 788 0.0 6 752 33 2 0 0 1 229 577 0.0 6 538 38 1 230 1432 0.0 6 1336 94 1 0 1 231 704 0.0 6 657 45 2 232 836 0.0 6 791 44 1 233 692 0.0 6 654 37 1 234 528 0.0 6 495 30 3 235 382 0.0 6 366 15 1 236 319 0.0 6 302 17 237 274 0.0 6 256 17 1 238 313 0.0 6 290 23 239 291 0.0 6 276 14 1 240 375 0.0 6 357 18 241 632 0.0 6 588 40 4 242 547 0.0 6 513 34 243 863 0.0 6 821 39 3 244 611 0.0 6 573 37 1 245 735 0.0 6 700 35 246 499 0.0 6 470 29 247 842 0.0 6 799 43 248 675 0.0 6 639 36 249 1014 0.0 6 953 59 2 250 811 0.0 6 753 57 1 251 1200 0.0 6 1139 58 2 1 252 587 0.0 6 563 24 253 685 0.0 6 662 22 1 254 568 0.0 6 534 33 1 255 1067 0.0 6 999 65 3 256 1456 0.0 6 1382 73 1 257 919 0.0 6 867 50 1 1 258 903 0.0 6 848 54 1 259 739 0.0 6 693 46 260 651 0.0 6 606 45 261 416 0.0 6 389 27 262 507 0.0 6 478 27 2 263 641 0.0 6 608 31 2 264 707 0.0 6 668 38 1 265 806 0.0 6 751 54 1 266 572 0.0 6 540 30 2 267 538 0.0 6 504 33 1 268 959 0.0 6 902 53 4 269 982 0.0 6 923 57 1 1 270 710 0.0 6 676 33 1 271 1077 0.0 6 1016 59 1 1 272 1714 0.0 6 1623 89 2 273 1539 0.0 6 1472 66 1 274 1700 0.0 6 1601 96 3 275 1256 0.0 6 1164 89 3 276 2609 0.0 6 2463 139 7 277 11366 0.0 6 10775 576 13 1 1 278 11061 0.0 6 10530 505 22 3 1 279 251 0.0 6 229 21 1 280 40 0.0 6 35 4 1 281 7 0.0 6 6 0 0 0 1 282 2 0.0 6 1 0 0 0 1 284 2 0.0 6 2 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 428 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 64 23 0.0 6 22 1 72 4 0.0 6 4 73 9 0.0 6 9 74 8 0.0 6 8 75 4 0.0 6 4 76 5 0.0 6 5 77 1 0.0 6 1 78 2 0.0 6 2 79 4 0.0 6 4 80 4 0.0 6 4 81 1 0.0 6 1 82 1 0.0 6 1 83 2 0.0 6 2 84 1 0.0 6 1 85 4 0.0 6 4 86 1 0.0 6 1 87 13 0.0 6 13 90 3 0.0 6 3 92 1 0.0 6 1 93 12 0.0 6 12 94 1 0.0 6 1 95 1 0.0 6 1 98 2 0.0 6 2 99 1 0.0 6 1 100 1 0.0 6 1 104 2 0.0 6 2 106 2 0.0 6 2 107 3 0.0 6 3 110 2 0.0 6 2 111 5 0.0 6 5 112 3 0.0 6 3 113 5 0.0 6 5 114 3 0.0 6 3 116 3 0.0 6 3 122 1 0.0 6 1 124 1 0.0 6 1 125 3 0.0 6 3 126 7 0.0 6 7 127 1 0.0 6 1 128 2 0.0 6 2 129 4 0.0 6 4 130 4 0.0 6 4 133 2 0.0 6 2 134 1 0.0 6 1 135 3 0.0 6 3 136 1 0.0 6 1 141 5 0.0 6 5 143 2 0.0 6 2 144 3 0.0 6 3 149 1 0.0 6 1 150 1 0.0 6 1 152 7 0.0 6 7 153 7 0.0 6 7 155 4 0.0 6 4 156 4 0.0 6 4 162 1 0.0 6 1 163 5 0.0 6 5 164 2 0.0 6 2 165 4 0.0 6 4 167 1 0.0 6 1 169 1 0.0 6 1 170 4 0.0 6 4 171 3 0.0 6 3 172 2 0.0 6 2 173 2 0.0 6 2 174 3 0.0 6 3 175 4 0.0 6 4 176 2 0.0 6 2 179 14 0.0 6 13 1 181 1 0.0 6 1 182 3 0.0 6 3 183 7 0.0 6 7 186 4 0.0 6 4 187 1 0.0 6 1 189 1 0.0 6 1 192 11 0.0 6 11 195 2 0.0 6 2 196 1 0.0 6 1 197 9 0.0 6 9 198 4 0.0 6 4 199 7 0.0 6 7 200 2 0.0 6 2 201 4 0.0 6 4 202 1 0.0 6 1 204 9 0.0 6 9 205 7 0.0 6 7 206 20 0.0 6 20 210 3 0.0 6 3 214 1 0.0 6 1 216 5 0.0 6 5 217 5 0.0 6 5 219 3 0.0 6 3 220 14 0.0 6 14 221 7 0.0 6 7 222 6 0.0 6 6 223 1 0.0 6 1 231 1 0.0 6 1 232 1 0.0 6 1 233 4 0.0 6 4 234 2 0.0 6 2 235 1 0.0 6 1 236 2 0.0 6 2 237 3 0.0 6 3 238 1 0.0 6 1 239 2 0.0 6 2 241 1 0.0 6 1 243 1 0.0 6 1 244 2 0.0 6 2 250 1 0.0 6 1 252 1 0.0 6 1 254 1 0.0 6 1 257 5 0.0 6 5 258 1 0.0 6 1 266 5 0.0 6 5 269 1 0.0 6 1 272 1 0.0 6 1 285 2 0.0 6 2 308 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 83413 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1306.8 0 0 2 5 1 81.7 1 1 6 2 20.4 1 1 1 8 2 1.3 2 1 1 9 1 0.3 2 0 1 10 6 0.1 3 5 1 11 2 0.0 3 2 13 8 0.0 3 6 2 14 15 0.0 4 12 1 1 1 16 12 0.0 4 10 2 17 20 0.0 5 17 2 1 18 18 0.0 5 14 4 19 11 0.0 5 10 1 20 79 0.0 6 77 2 21 70 0.0 6 63 7 22 42 0.0 6 40 2 23 37 0.0 6 32 5 24 20 0.0 6 19 1 25 28 0.0 6 26 2 26 21 0.0 6 20 1 27 24 0.0 6 20 3 1 28 17 0.0 6 16 1 29 17 0.0 6 16 0 1 30 23 0.0 6 18 3 2 31 10 0.0 6 9 1 32 4 0.0 6 4 33 15 0.0 6 14 0 1 34 23 0.0 6 23 35 20 0.0 6 19 1 36 5 0.0 6 5 37 18 0.0 6 17 1 38 14 0.0 6 13 1 39 15 0.0 6 14 1 40 22 0.0 6 22 41 12 0.0 6 10 2 42 15 0.0 6 13 1 0 1 43 21 0.0 6 17 3 1 44 23 0.0 6 20 3 45 14 0.0 6 12 2 46 39 0.0 6 36 2 0 0 0 1 47 35 0.0 6 26 7 2 48 15 0.0 6 11 4 49 18 0.0 6 18 50 21 0.0 6 17 3 0 1 51 16 0.0 6 12 3 0 0 1 52 41 0.0 6 36 2 2 1 53 20 0.0 6 18 2 54 35 0.0 6 32 3 55 29 0.0 6 24 4 1 56 24 0.0 6 22 1 1 57 34 0.0 6 28 5 1 58 27 0.0 6 23 4 59 15 0.0 6 9 5 1 60 25 0.0 6 20 5 61 17 0.0 6 15 2 62 15 0.0 6 14 1 63 21 0.0 6 19 1 1 64 29 0.0 6 26 3 65 22 0.0 6 16 4 2 66 36 0.0 6 30 4 1 0 1 67 163 0.0 6 149 13 1 68 51 0.0 6 45 5 1 69 59 0.0 6 57 2 70 22 0.0 6 18 4 71 55 0.0 6 47 7 0 0 1 72 43 0.0 6 38 4 0 0 1 73 24 0.0 6 22 2 74 22 0.0 6 17 5 75 33 0.0 6 33 76 40 0.0 6 37 3 77 35 0.0 6 32 2 0 1 78 51 0.0 6 41 10 79 51 0.0 6 48 3 80 32 0.0 6 29 2 1 81 26 0.0 6 24 2 82 38 0.0 6 34 3 1 83 62 0.0 6 57 5 84 58 0.0 6 52 4 1 1 85 25 0.0 6 22 3 86 23 0.0 6 22 1 87 66 0.0 6 60 5 1 88 45 0.0 6 38 6 1 89 53 0.0 6 49 3 1 90 51 0.0 6 43 7 1 91 46 0.0 6 42 3 1 92 37 0.0 6 33 3 1 93 66 0.0 6 60 6 94 82 0.0 6 76 5 1 95 62 0.0 6 51 10 1 96 63 0.0 6 57 6 97 65 0.0 6 62 3 98 61 0.0 6 58 2 1 99 69 0.0 6 64 4 1 100 76 0.0 6 69 6 1 101 84 0.0 6 75 6 3 102 114 0.0 6 105 7 1 0 1 103 133 0.0 6 123 9 0 0 1 104 100 0.0 6 94 6 105 104 0.0 6 97 5 2 106 48 0.0 6 46 2 107 58 0.0 6 55 3 108 71 0.0 6 64 6 0 0 0 0 1 109 73 0.0 6 65 7 0 0 0 0 1 110 79 0.0 6 70 9 111 94 0.0 6 89 3 2 112 94 0.0 6 84 7 2 1 113 122 0.0 6 112 10 114 96 0.0 6 87 8 1 115 74 0.0 6 70 4 116 82 0.0 6 77 5 117 61 0.0 6 55 6 118 97 0.0 6 94 2 0 1 119 139 0.0 6 128 8 2 1 120 222 0.0 6 212 10 121 125 0.0 6 113 11 0 0 0 0 1 122 199 0.0 6 184 12 1 0 2 123 221 0.0 6 214 7 124 257 0.0 6 236 20 1 125 289 0.0 6 273 14 1 0 0 1 126 182 0.0 6 169 12 1 127 205 0.0 6 195 9 1 128 111 0.0 6 103 5 1 0 2 129 112 0.0 6 108 4 130 163 0.0 6 156 7 131 254 0.0 6 247 7 132 232 0.0 6 223 8 1 133 291 0.0 6 271 16 2 1 1 134 512 0.0 6 496 14 2 135 458 0.0 6 441 16 0 0 1 136 392 0.0 6 371 19 1 0 1 137 325 0.0 6 313 10 0 1 1 138 406 0.0 6 386 18 1 0 1 139 390 0.0 6 370 18 2 140 414 0.0 6 392 21 1 141 534 0.0 6 512 20 2 142 538 0.0 6 513 23 2 143 591 0.0 6 556 32 0 0 3 144 458 0.0 6 441 15 1 0 1 145 333 0.0 6 320 13 146 354 0.0 6 338 16 147 382 0.0 6 368 14 148 581 0.0 6 553 25 3 149 816 0.0 6 778 37 1 150 719 0.0 6 696 23 151 610 0.0 6 579 29 0 1 0 0 1 152 810 0.0 6 774 34 1 1 153 641 0.0 6 617 24 154 565 0.0 6 544 18 2 0 0 0 1 155 672 0.0 6 641 30 0 0 1 156 875 0.0 6 829 39 5 1 1 157 868 0.0 6 830 36 1 1 158 682 0.0 6 649 29 2 1 0 0 1 159 736 0.0 6 712 23 0 1 160 598 0.0 6 574 22 2 161 456 0.0 6 437 18 1 162 481 0.0 6 455 24 2 163 733 0.0 6 701 32 164 683 0.0 6 651 29 0 0 3 165 988 0.0 6 962 22 3 0 0 0 1 166 767 0.0 6 738 27 0 0 1 0 1 167 930 0.0 6 891 36 2 0 1 168 656 0.0 6 634 22 169 482 0.0 6 463 19 170 830 0.0 6 792 36 0 0 2 171 655 0.0 6 636 19 172 638 0.0 6 599 38 1 173 987 0.0 6 949 34 3 0 1 174 721 0.0 6 688 32 1 175 984 0.0 6 948 36 176 1010 0.0 6 966 42 1 0 1 177 851 0.0 6 819 31 1 178 1149 0.0 6 1097 52 179 1123 0.0 6 1083 38 1 0 1 180 2070 0.0 6 1985 81 2 0 2 181 1014 0.0 6 973 40 1 182 835 0.0 6 790 44 0 0 1 183 2641 0.0 6 2551 83 3 1 3 184 1573 0.0 6 1503 67 1 0 2 185 1715 0.0 6 1645 66 3 0 1 186 1329 0.0 6 1283 45 1 187 1473 0.0 6 1407 59 3 2 2 188 1378 0.0 6 1322 50 2 1 2 1 189 982 0.0 6 942 40 190 1268 0.0 6 1221 47 191 1897 0.0 6 1817 73 4 1 2 192 909 0.0 6 872 33 3 1 193 588 0.0 6 566 20 1 0 1 194 1000 0.0 6 952 43 3 1 0 1 195 964 0.0 6 916 45 2 1 196 2367 0.0 6 2299 64 3 0 1 197 952 0.0 6 905 41 5 0 1 198 1422 0.0 6 1365 53 4 199 1905 0.0 6 1839 63 2 0 1 200 1032 0.0 6 983 47 1 0 1 201 1704 0.0 6 1631 70 1 0 2 202 843 0.0 6 812 31 203 1045 0.0 6 1006 36 3 204 971 0.0 6 940 30 0 0 1 205 969 0.0 6 935 34 206 528 0.0 6 512 15 1 207 517 0.0 6 495 21 0 1 208 670 0.0 6 647 22 1 209 352 0.0 6 338 11 2 0 1 210 290 0.0 6 280 10 211 233 0.0 6 225 8 212 426 0.0 6 410 15 0 0 1 213 217 0.0 6 210 6 0 1 214 491 0.0 6 475 15 1 215 549 0.0 6 530 19 216 466 0.0 6 451 14 0 0 1 217 386 0.0 6 371 15 218 276 0.0 6 269 7 219 307 0.0 6 295 12 220 169 0.0 6 160 9 221 430 0.0 6 416 14 222 328 0.0 6 314 14 223 457 0.0 6 434 23 224 224 0.0 6 217 6 0 0 1 225 370 0.0 6 358 12 226 164 0.0 6 159 5 227 187 0.0 6 182 5 228 114 0.0 6 108 6 229 293 0.0 6 282 10 0 0 1 230 126 0.0 6 121 4 0 0 1 231 220 0.0 6 214 6 232 247 0.0 6 235 10 1 1 233 158 0.0 6 152 6 234 104 0.0 6 102 2 235 60 0.0 6 58 2 236 35 0.0 6 35 237 31 0.0 6 30 1 238 30 0.0 6 29 1 239 49 0.0 6 47 2 240 69 0.0 6 66 3 241 138 0.0 6 135 1 2 242 127 0.0 6 125 2 243 134 0.0 6 125 9 244 87 0.0 6 84 3 245 48 0.0 6 44 4 246 38 0.0 6 37 1 247 21 0.0 6 20 1 248 58 0.0 6 54 4 249 13 0.0 6 11 1 0 0 1 250 12 0.0 6 12 251 23 0.0 6 23 252 4 0.0 6 4 253 4 0.0 6 4 254 3 0.0 6 3 256 1 0.0 6 1 277 3 0.0 6 3 278 33 0.0 6 31 2 279 268 0.0 6 240 27 1 280 10 0.0 6 8 2 281 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 128 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 58 1 0.0 6 1 71 2 0.0 6 2 72 4 0.0 6 4 73 4 0.0 6 4 74 5 0.0 6 5 75 1 0.0 6 1 76 1 0.0 6 1 78 7 0.0 6 7 79 1 0.0 6 1 80 3 0.0 6 3 83 1 0.0 6 1 84 2 0.0 6 2 85 2 0.0 6 2 86 7 0.0 6 7 91 2 0.0 6 2 92 4 0.0 6 4 95 1 0.0 6 1 98 1 0.0 6 1 104 1 0.0 6 1 106 3 0.0 6 3 109 2 0.0 6 2 110 8 0.0 6 8 111 2 0.0 6 2 112 1 0.0 6 1 113 2 0.0 6 2 124 1 0.0 6 1 125 3 0.0 6 3 128 4 0.0 6 4 129 4 0.0 6 4 141 1 0.0 6 0 0 0 0 0 0 1 143 1 0.0 6 1 145 1 0.0 6 1 151 4 0.0 6 4 152 5 0.0 6 5 153 1 0.0 6 1 154 2 0.0 6 2 155 1 0.0 6 1 158 1 0.0 6 1 161 4 0.0 6 4 162 2 0.0 6 2 164 1 0.0 6 1 169 1 0.0 6 1 170 1 0.0 6 1 172 1 0.0 6 1 173 1 0.0 6 1 174 2 0.0 6 2 177 1 0.0 6 1 178 2 0.0 6 2 179 1 0.0 6 1 181 2 0.0 6 2 182 1 0.0 6 1 191 2 0.0 6 2 192 1 0.0 6 1 197 1 0.0 6 1 198 3 0.0 6 3 201 1 0.0 6 1 204 1 0.0 6 1 205 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_08_221_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_08_448_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_08_221_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_08_448_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 3.65 s (62 µs/read; 0.97 M reads/minute). === Summary === Total read pairs processed: 58,870 Read 1 with adapter: 58,862 (100.0%) Read 2 with adapter: 58,870 (100.0%) == Read fate breakdown == Pairs that were too short: 23 (0.0%) Pairs discarded as untrimmed: 8 (0.0%) Pairs written (passing filters): 58,839 (99.9%) Total basepairs processed: 24,524,508 bp Read 1: 13,595,031 bp Read 2: 10,929,477 bp Total written (filtered): 3,913,990 bp (16.0%) Read 1: 2,041,916 bp Read 2: 1,872,074 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 56955 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 919.8 0 1 4 3 230.0 1 1 2 6 1 14.4 1 0 0 1 7 1 3.6 2 1 8 1 0.9 2 1 10 1 0.1 3 1 11 1 0.0 3 1 13 1 0.0 3 1 16 1 0.0 4 1 17 3 0.0 5 2 0 1 18 1 0.0 5 1 19 2 0.0 5 1 1 20 65 0.0 6 64 1 21 31 0.0 6 28 3 22 32 0.0 6 31 1 23 26 0.0 6 25 1 24 14 0.0 6 13 1 25 22 0.0 6 21 0 1 26 13 0.0 6 11 1 0 0 0 1 27 12 0.0 6 12 28 12 0.0 6 11 1 29 7 0.0 6 7 30 6 0.0 6 5 0 0 0 0 1 31 7 0.0 6 7 32 4 0.0 6 4 33 6 0.0 6 6 34 11 0.0 6 11 35 8 0.0 6 7 0 0 0 0 0 1 36 4 0.0 6 4 37 2 0.0 6 2 38 3 0.0 6 3 39 6 0.0 6 5 1 40 2 0.0 6 2 41 5 0.0 6 4 1 42 3 0.0 6 2 1 43 1 0.0 6 1 44 7 0.0 6 7 45 7 0.0 6 7 46 3 0.0 6 2 1 47 4 0.0 6 4 48 4 0.0 6 4 49 7 0.0 6 7 50 1 0.0 6 1 51 1 0.0 6 0 1 52 4 0.0 6 4 53 3 0.0 6 3 54 7 0.0 6 6 0 1 55 3 0.0 6 3 56 1 0.0 6 1 57 2 0.0 6 2 58 6 0.0 6 6 59 5 0.0 6 5 60 2 0.0 6 2 61 1 0.0 6 1 62 2 0.0 6 2 63 4 0.0 6 3 1 64 5 0.0 6 4 1 65 4 0.0 6 4 66 2 0.0 6 1 1 67 3 0.0 6 3 68 5 0.0 6 5 69 6 0.0 6 6 70 5 0.0 6 4 1 71 13 0.0 6 12 1 72 8 0.0 6 8 73 10 0.0 6 10 74 11 0.0 6 11 75 8 0.0 6 8 76 10 0.0 6 10 77 5 0.0 6 5 78 6 0.0 6 6 79 11 0.0 6 11 80 10 0.0 6 9 1 81 16 0.0 6 15 1 82 13 0.0 6 12 1 83 11 0.0 6 11 84 8 0.0 6 8 85 8 0.0 6 8 86 6 0.0 6 6 87 6 0.0 6 6 88 15 0.0 6 14 1 89 4 0.0 6 4 90 6 0.0 6 5 1 91 8 0.0 6 8 92 8 0.0 6 8 93 8 0.0 6 8 94 11 0.0 6 11 95 8 0.0 6 8 96 10 0.0 6 8 2 97 5 0.0 6 5 98 10 0.0 6 10 99 14 0.0 6 14 100 15 0.0 6 14 1 101 11 0.0 6 11 102 13 0.0 6 12 1 103 7 0.0 6 6 1 104 16 0.0 6 16 105 16 0.0 6 16 106 15 0.0 6 14 1 107 13 0.0 6 13 108 9 0.0 6 8 1 109 19 0.0 6 19 110 25 0.0 6 24 1 111 21 0.0 6 21 112 18 0.0 6 18 113 38 0.0 6 37 1 114 31 0.0 6 30 0 0 1 115 19 0.0 6 19 116 22 0.0 6 21 1 117 26 0.0 6 25 0 0 0 1 118 21 0.0 6 21 119 29 0.0 6 28 0 1 120 22 0.0 6 20 2 121 24 0.0 6 24 122 16 0.0 6 16 123 26 0.0 6 25 1 124 35 0.0 6 35 125 27 0.0 6 27 126 36 0.0 6 36 127 41 0.0 6 41 128 45 0.0 6 44 1 129 40 0.0 6 39 0 1 130 33 0.0 6 33 131 28 0.0 6 28 132 32 0.0 6 32 133 42 0.0 6 42 134 37 0.0 6 36 0 0 0 0 1 135 47 0.0 6 46 1 136 50 0.0 6 50 137 46 0.0 6 46 138 66 0.0 6 66 139 71 0.0 6 70 1 140 67 0.0 6 67 141 128 0.0 6 127 1 142 249 0.0 6 247 2 143 376 0.0 6 375 1 144 537 0.0 6 536 1 145 567 0.0 6 564 2 0 1 146 550 0.0 6 550 147 812 0.0 6 810 2 148 693 0.0 6 693 149 656 0.0 6 655 1 150 628 0.0 6 628 151 760 0.0 6 760 152 846 0.0 6 846 153 547 0.0 6 545 2 154 466 0.0 6 465 1 155 481 0.0 6 479 2 156 402 0.0 6 402 157 354 0.0 6 353 1 158 438 0.0 6 438 159 439 0.0 6 438 1 160 440 0.0 6 437 3 161 485 0.0 6 484 1 162 546 0.0 6 543 3 163 474 0.0 6 473 1 164 422 0.0 6 420 2 165 422 0.0 6 420 1 0 0 0 1 166 364 0.0 6 362 2 167 332 0.0 6 332 168 372 0.0 6 370 1 1 169 379 0.0 6 379 170 360 0.0 6 360 171 419 0.0 6 418 1 172 486 0.0 6 484 1 0 0 0 1 173 410 0.0 6 408 2 174 426 0.0 6 424 1 0 0 0 1 175 363 0.0 6 361 2 176 283 0.0 6 281 2 177 289 0.0 6 288 1 178 288 0.0 6 287 1 179 333 0.0 6 331 2 180 807 0.0 6 805 2 181 395 0.0 6 393 2 182 333 0.0 6 331 2 183 371 0.0 6 370 1 184 301 0.0 6 296 5 185 331 0.0 6 328 3 186 363 0.0 6 361 1 0 1 187 315 0.0 6 314 1 188 363 0.0 6 359 3 1 189 319 0.0 6 318 1 190 317 0.0 6 315 2 191 320 0.0 6 319 1 192 428 0.0 6 424 4 193 449 0.0 6 447 2 194 476 0.0 6 470 5 0 0 0 0 1 195 627 0.0 6 619 8 196 607 0.0 6 604 3 197 580 0.0 6 573 7 198 532 0.0 6 529 3 199 391 0.0 6 387 4 200 461 0.0 6 460 1 201 423 0.0 6 423 202 503 0.0 6 496 6 1 203 537 0.0 6 534 2 0 0 1 204 628 0.0 6 625 3 205 680 0.0 6 672 7 1 206 504 0.0 6 498 6 207 592 0.0 6 584 6 1 1 208 452 0.0 6 449 2 0 1 209 420 0.0 6 416 4 210 545 0.0 6 543 2 211 559 0.0 6 556 2 0 0 1 212 537 0.0 6 536 1 213 642 0.0 6 637 4 0 0 0 0 1 214 756 0.0 6 748 7 0 0 1 215 544 0.0 6 541 2 1 216 596 0.0 6 592 4 217 618 0.0 6 613 4 1 218 539 0.0 6 535 4 219 435 0.0 6 430 4 0 0 0 1 220 427 0.0 6 424 3 221 484 0.0 6 483 0 0 1 222 526 0.0 6 525 0 0 0 0 1 223 487 0.0 6 483 3 0 0 1 224 491 0.0 6 486 4 1 225 505 0.0 6 502 3 226 464 0.0 6 460 4 227 409 0.0 6 409 228 478 0.0 6 475 3 229 414 0.0 6 411 3 230 399 0.0 6 398 1 231 437 0.0 6 433 4 232 408 0.0 6 406 2 233 329 0.0 6 329 234 371 0.0 6 370 1 235 341 0.0 6 339 1 0 1 236 301 0.0 6 301 237 372 0.0 6 370 1 0 0 0 0 1 238 353 0.0 6 352 1 239 343 0.0 6 341 2 240 362 0.0 6 359 3 241 279 0.0 6 277 2 242 313 0.0 6 309 4 243 274 0.0 6 272 2 244 264 0.0 6 261 3 245 229 0.0 6 226 2 0 0 0 0 1 246 190 0.0 6 189 1 247 213 0.0 6 212 0 1 248 193 0.0 6 192 1 249 185 0.0 6 184 1 250 184 0.0 6 183 1 251 174 0.0 6 172 2 252 186 0.0 6 185 1 253 156 0.0 6 154 2 254 144 0.0 6 143 1 255 186 0.0 6 185 1 256 155 0.0 6 155 257 138 0.0 6 138 258 168 0.0 6 165 3 259 154 0.0 6 153 1 260 134 0.0 6 132 2 261 104 0.0 6 103 1 262 110 0.0 6 107 2 0 0 0 1 263 131 0.0 6 131 264 101 0.0 6 100 1 265 118 0.0 6 116 2 266 114 0.0 6 114 267 124 0.0 6 123 1 268 125 0.0 6 122 3 269 80 0.0 6 76 4 270 70 0.0 6 69 1 271 85 0.0 6 82 2 1 272 142 0.0 6 139 3 273 152 0.0 6 151 1 274 169 0.0 6 167 2 275 133 0.0 6 132 1 276 287 0.0 6 286 1 277 1081 0.0 6 1060 19 0 1 0 0 1 278 1838 0.0 6 1796 40 2 279 44 0.0 6 43 1 280 3 0.0 6 3 281 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 1907 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 3 0.0 6 3 64 158 0.0 6 150 8 66 3 0.0 6 3 70 1 0.0 6 0 1 71 8 0.0 6 7 1 72 4 0.0 6 4 73 8 0.0 6 8 74 14 0.0 6 13 0 1 75 21 0.0 6 21 76 24 0.0 6 24 77 22 0.0 6 22 78 30 0.0 6 30 79 17 0.0 6 17 80 24 0.0 6 24 81 19 0.0 6 19 82 33 0.0 6 32 1 83 32 0.0 6 32 84 26 0.0 6 26 85 15 0.0 6 15 86 18 0.0 6 17 0 0 0 0 0 1 87 18 0.0 6 18 88 15 0.0 6 15 89 16 0.0 6 16 90 9 0.0 6 9 91 16 0.0 6 16 92 22 0.0 6 22 93 21 0.0 6 21 94 22 0.0 6 22 95 7 0.0 6 7 96 20 0.0 6 20 97 19 0.0 6 19 98 12 0.0 6 12 99 9 0.0 6 9 100 21 0.0 6 21 101 13 0.0 6 13 102 13 0.0 6 13 103 10 0.0 6 10 104 20 0.0 6 20 105 19 0.0 6 19 106 13 0.0 6 13 107 12 0.0 6 12 108 11 0.0 6 11 109 10 0.0 6 10 110 16 0.0 6 16 111 29 0.0 6 29 112 13 0.0 6 13 113 10 0.0 6 10 114 11 0.0 6 11 115 11 0.0 6 11 116 10 0.0 6 10 117 6 0.0 6 6 118 9 0.0 6 9 119 9 0.0 6 9 120 3 0.0 6 3 121 12 0.0 6 12 122 4 0.0 6 4 123 11 0.0 6 11 124 8 0.0 6 8 125 2 0.0 6 2 126 9 0.0 6 9 127 7 0.0 6 7 128 7 0.0 6 7 129 9 0.0 6 9 130 5 0.0 6 5 131 3 0.0 6 3 132 4 0.0 6 4 133 8 0.0 6 8 134 13 0.0 6 13 135 17 0.0 6 17 136 6 0.0 6 6 137 8 0.0 6 8 138 9 0.0 6 9 139 5 0.0 6 5 140 7 0.0 6 7 141 10 0.0 6 10 142 10 0.0 6 10 143 7 0.0 6 7 144 12 0.0 6 12 145 8 0.0 6 8 146 8 0.0 6 8 147 7 0.0 6 7 148 5 0.0 6 5 149 10 0.0 6 10 150 10 0.0 6 10 151 8 0.0 6 8 152 6 0.0 6 6 153 9 0.0 6 9 154 10 0.0 6 10 155 8 0.0 6 8 156 8 0.0 6 8 157 9 0.0 6 9 158 15 0.0 6 15 159 17 0.0 6 17 160 11 0.0 6 11 161 10 0.0 6 10 162 5 0.0 6 5 163 10 0.0 6 10 164 7 0.0 6 7 165 16 0.0 6 16 166 16 0.0 6 16 167 13 0.0 6 13 168 8 0.0 6 8 169 10 0.0 6 10 170 11 0.0 6 11 171 9 0.0 6 8 1 172 4 0.0 6 4 173 4 0.0 6 4 174 6 0.0 6 6 175 6 0.0 6 6 176 13 0.0 6 13 177 5 0.0 6 5 178 14 0.0 6 14 179 13 0.0 6 13 180 5 0.0 6 5 181 4 0.0 6 4 182 5 0.0 6 5 183 7 0.0 6 7 184 4 0.0 6 4 185 7 0.0 6 7 186 14 0.0 6 14 187 16 0.0 6 16 188 4 0.0 6 4 189 10 0.0 6 10 190 14 0.0 6 14 191 7 0.0 6 7 192 8 0.0 6 8 193 11 0.0 6 11 194 5 0.0 6 5 195 10 0.0 6 10 196 7 0.0 6 7 197 5 0.0 6 5 198 5 0.0 6 5 199 4 0.0 6 4 200 5 0.0 6 5 201 9 0.0 6 9 202 9 0.0 6 9 203 5 0.0 6 5 204 9 0.0 6 9 205 9 0.0 6 9 206 11 0.0 6 11 207 2 0.0 6 2 208 6 0.0 6 6 209 4 0.0 6 4 210 7 0.0 6 7 211 3 0.0 6 3 212 3 0.0 6 3 213 7 0.0 6 7 214 7 0.0 6 7 215 5 0.0 6 5 216 1 0.0 6 1 217 3 0.0 6 3 218 7 0.0 6 7 219 4 0.0 6 4 220 6 0.0 6 6 221 5 0.0 6 5 222 3 0.0 6 3 223 8 0.0 6 7 1 224 5 0.0 6 5 225 5 0.0 6 5 226 3 0.0 6 3 227 3 0.0 6 3 228 7 0.0 6 7 229 8 0.0 6 8 230 4 0.0 6 4 231 5 0.0 6 5 232 3 0.0 6 3 233 5 0.0 6 5 234 1 0.0 6 1 235 4 0.0 6 4 236 3 0.0 6 3 237 2 0.0 6 2 238 1 0.0 6 1 239 5 0.0 6 5 240 1 0.0 6 1 241 3 0.0 6 3 242 4 0.0 6 4 243 5 0.0 6 5 244 3 0.0 6 3 245 2 0.0 6 2 246 2 0.0 6 2 247 6 0.0 6 6 248 3 0.0 6 3 249 7 0.0 6 7 250 3 0.0 6 3 251 1 0.0 6 1 252 1 0.0 6 1 254 3 0.0 6 3 255 1 0.0 6 1 256 1 0.0 6 1 257 2 0.0 6 2 258 2 0.0 6 2 259 1 0.0 6 1 260 4 0.0 6 4 262 1 0.0 6 1 263 1 0.0 6 1 264 1 0.0 6 1 265 1 0.0 6 1 266 2 0.0 6 2 268 1 0.0 6 1 272 1 0.0 6 1 273 2 0.0 6 2 275 2 0.0 6 2 277 1 0.0 6 1 279 1 0.0 6 1 281 1 0.0 6 1 282 2 0.0 6 2 283 1 0.0 6 1 291 1 0.0 6 1 297 1 0.0 6 1 307 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 57504 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 6 2 14.4 1 2 10 3 0.1 3 3 11 4 0.0 3 4 12 7 0.0 3 6 1 13 11 0.0 3 10 1 14 11 0.0 4 11 15 1 0.0 4 0 1 16 5 0.0 4 5 17 21 0.0 5 20 1 18 18 0.0 5 16 2 19 13 0.0 5 13 20 43 0.0 6 42 1 21 38 0.0 6 36 2 22 17 0.0 6 15 2 23 17 0.0 6 15 1 1 24 15 0.0 6 15 25 18 0.0 6 16 1 1 26 12 0.0 6 11 0 1 27 18 0.0 6 16 2 28 10 0.0 6 10 29 9 0.0 6 9 30 9 0.0 6 8 1 31 16 0.0 6 16 32 7 0.0 6 7 33 14 0.0 6 12 2 34 7 0.0 6 5 2 35 7 0.0 6 5 2 36 10 0.0 6 9 1 37 9 0.0 6 9 38 4 0.0 6 4 39 10 0.0 6 9 1 40 15 0.0 6 14 1 41 10 0.0 6 10 42 10 0.0 6 9 1 43 21 0.0 6 20 0 1 44 15 0.0 6 14 1 45 12 0.0 6 11 1 46 15 0.0 6 15 47 11 0.0 6 10 1 48 17 0.0 6 16 0 1 49 17 0.0 6 16 1 50 16 0.0 6 14 2 51 9 0.0 6 7 1 0 1 52 9 0.0 6 8 0 1 53 21 0.0 6 19 2 54 18 0.0 6 16 2 55 23 0.0 6 22 1 56 16 0.0 6 14 1 0 0 1 57 23 0.0 6 20 3 58 26 0.0 6 24 1 0 1 59 15 0.0 6 14 1 60 22 0.0 6 22 61 21 0.0 6 19 2 62 23 0.0 6 22 1 63 27 0.0 6 27 64 41 0.0 6 40 1 65 33 0.0 6 33 66 26 0.0 6 26 67 40 0.0 6 39 1 68 111 0.0 6 108 2 0 0 1 69 39 0.0 6 38 1 70 64 0.0 6 60 3 1 71 27 0.0 6 27 72 40 0.0 6 38 2 73 42 0.0 6 41 1 74 27 0.0 6 27 75 31 0.0 6 30 1 76 33 0.0 6 29 3 0 0 1 77 33 0.0 6 30 3 78 37 0.0 6 34 2 0 0 0 1 79 31 0.0 6 31 80 42 0.0 6 40 2 81 52 0.0 6 52 82 61 0.0 6 59 2 83 55 0.0 6 51 4 84 65 0.0 6 63 2 85 31 0.0 6 31 86 37 0.0 6 35 2 87 59 0.0 6 59 88 45 0.0 6 43 2 89 46 0.0 6 43 2 0 0 0 1 90 48 0.0 6 46 2 91 50 0.0 6 48 0 0 0 0 1 1 92 58 0.0 6 55 3 93 63 0.0 6 58 5 94 83 0.0 6 81 2 95 66 0.0 6 63 3 96 74 0.0 6 72 2 97 75 0.0 6 74 1 98 58 0.0 6 57 1 99 63 0.0 6 60 3 100 65 0.0 6 64 0 0 0 0 0 1 101 67 0.0 6 64 3 102 84 0.0 6 80 2 1 0 0 0 1 103 79 0.0 6 77 1 1 104 138 0.0 6 136 1 1 105 133 0.0 6 126 6 0 0 1 106 97 0.0 6 95 2 107 75 0.0 6 72 2 1 108 90 0.0 6 88 2 109 90 0.0 6 88 1 0 0 0 0 1 110 112 0.0 6 108 3 0 1 111 126 0.0 6 124 1 0 0 0 1 112 136 0.0 6 132 2 0 0 1 1 113 164 0.0 6 155 8 1 114 122 0.0 6 117 5 115 140 0.0 6 137 3 116 147 0.0 6 144 3 117 118 0.0 6 117 1 118 161 0.0 6 157 4 119 175 0.0 6 175 120 169 0.0 6 167 2 121 136 0.0 6 135 1 122 167 0.0 6 163 3 0 0 0 0 1 123 159 0.0 6 156 3 124 207 0.0 6 206 1 125 230 0.0 6 225 5 126 287 0.0 6 284 2 1 127 247 0.0 6 243 2 1 0 1 128 290 0.0 6 282 7 1 129 314 0.0 6 310 4 130 327 0.0 6 320 7 131 376 0.0 6 373 2 0 0 0 0 1 132 436 0.0 6 425 11 133 413 0.0 6 404 7 0 0 1 1 134 429 0.0 6 424 5 135 462 0.0 6 449 12 0 0 0 0 1 136 426 0.0 6 414 10 0 1 0 1 137 432 0.0 6 424 5 1 0 2 138 413 0.0 6 408 5 139 466 0.0 6 460 4 0 1 0 0 1 140 504 0.0 6 501 3 141 665 0.0 6 660 4 0 0 1 142 876 0.0 6 870 2 2 1 1 143 1024 0.0 6 1014 9 1 144 936 0.0 6 928 5 0 1 0 1 1 145 1033 0.0 6 1022 9 1 0 0 0 1 146 1207 0.0 6 1190 12 1 2 2 147 1093 0.0 6 1080 9 0 1 2 1 148 1201 0.0 6 1193 6 0 0 1 1 149 1300 0.0 6 1290 8 1 0 0 0 1 150 1350 0.0 6 1336 9 2 0 1 1 1 151 1793 0.0 6 1777 15 0 0 0 0 1 152 1265 0.0 6 1252 11 1 1 153 1293 0.0 6 1277 14 0 0 0 2 154 1301 0.0 6 1285 13 1 0 0 2 155 977 0.0 6 968 7 1 0 0 1 156 1087 0.0 6 1074 10 0 0 3 157 993 0.0 6 982 8 1 0 0 1 1 158 1077 0.0 6 1067 8 1 0 1 159 1031 0.0 6 1025 4 0 1 1 160 990 0.0 6 980 8 0 0 0 1 1 161 929 0.0 6 924 5 162 828 0.0 6 818 10 163 804 0.0 6 796 7 0 0 0 0 1 164 784 0.0 6 778 6 165 708 0.0 6 700 6 2 166 749 0.0 6 741 7 0 0 0 1 167 814 0.0 6 804 10 168 718 0.0 6 708 7 0 1 1 0 1 169 866 0.0 6 858 7 0 0 0 0 1 170 705 0.0 6 700 4 0 0 0 0 1 171 744 0.0 6 736 7 1 172 728 0.0 6 724 3 0 0 1 173 742 0.0 6 732 6 0 2 1 1 174 707 0.0 6 702 5 175 627 0.0 6 624 1 0 1 0 0 1 176 618 0.0 6 614 4 177 671 0.0 6 666 4 0 0 0 0 1 178 533 0.0 6 531 1 0 0 1 179 584 0.0 6 574 8 0 1 1 180 406 0.0 6 405 1 181 394 0.0 6 390 3 1 182 373 0.0 6 369 3 0 0 0 1 183 380 0.0 6 376 3 0 0 0 1 184 477 0.0 6 475 2 185 677 0.0 6 672 5 186 571 0.0 6 565 3 1 1 0 0 1 187 371 0.0 6 365 4 2 188 388 0.0 6 384 1 2 1 189 361 0.0 6 359 2 190 388 0.0 6 384 4 191 348 0.0 6 346 2 192 348 0.0 6 345 2 0 0 1 193 224 0.0 6 223 1 194 255 0.0 6 252 3 195 210 0.0 6 203 7 196 277 0.0 6 269 8 197 250 0.0 6 245 4 0 0 1 198 265 0.0 6 260 5 199 247 0.0 6 245 2 200 184 0.0 6 182 2 201 188 0.0 6 184 3 1 202 121 0.0 6 115 6 203 109 0.0 6 106 3 204 109 0.0 6 108 0 1 205 83 0.0 6 82 1 206 82 0.0 6 82 207 70 0.0 6 69 1 208 80 0.0 6 76 3 0 0 0 1 209 74 0.0 6 74 210 69 0.0 6 68 1 211 57 0.0 6 55 2 212 55 0.0 6 55 213 58 0.0 6 57 1 214 71 0.0 6 69 2 215 61 0.0 6 61 216 61 0.0 6 59 2 217 59 0.0 6 58 1 218 44 0.0 6 43 1 219 52 0.0 6 52 220 23 0.0 6 21 2 221 35 0.0 6 34 1 222 19 0.0 6 17 2 223 27 0.0 6 26 1 224 21 0.0 6 20 1 225 28 0.0 6 28 226 25 0.0 6 24 1 227 31 0.0 6 29 2 228 16 0.0 6 15 1 229 25 0.0 6 25 230 17 0.0 6 17 231 13 0.0 6 13 232 25 0.0 6 25 233 17 0.0 6 14 3 234 4 0.0 6 4 235 6 0.0 6 6 236 3 0.0 6 3 238 5 0.0 6 5 239 5 0.0 6 3 1 0 0 0 0 1 240 6 0.0 6 6 241 8 0.0 6 8 242 8 0.0 6 8 243 6 0.0 6 6 244 8 0.0 6 8 245 4 0.0 6 4 246 5 0.0 6 5 247 4 0.0 6 4 248 3 0.0 6 3 249 2 0.0 6 2 250 1 0.0 6 1 254 1 0.0 6 1 255 1 0.0 6 1 257 1 0.0 6 1 278 1 0.0 6 1 279 8 0.0 6 8 280 51 0.0 6 51 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 1366 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 6 47 14.4 1 0 0 47 53 1 0.0 6 1 55 1 0.0 6 1 62 3 0.0 6 3 63 1 0.0 6 1 64 1 0.0 6 1 65 5 0.0 6 5 67 3 0.0 6 3 68 13 0.0 6 13 69 1 0.0 6 1 70 14 0.0 6 13 1 71 12 0.0 6 12 72 17 0.0 6 17 73 17 0.0 6 17 74 26 0.0 6 26 75 23 0.0 6 23 76 29 0.0 6 29 77 20 0.0 6 20 78 24 0.0 6 23 0 0 0 1 79 21 0.0 6 21 80 37 0.0 6 37 81 33 0.0 6 31 0 0 0 1 0 1 82 7 0.0 6 7 83 14 0.0 6 14 84 14 0.0 6 13 0 0 0 1 85 12 0.0 6 12 86 8 0.0 6 8 87 17 0.0 6 17 88 7 0.0 6 7 89 11 0.0 6 11 90 32 0.0 6 32 91 26 0.0 6 26 92 18 0.0 6 18 93 15 0.0 6 15 94 19 0.0 6 19 95 13 0.0 6 12 0 0 0 1 96 7 0.0 6 7 97 11 0.0 6 11 98 11 0.0 6 11 99 14 0.0 6 12 0 0 0 2 100 10 0.0 6 10 101 13 0.0 6 12 1 102 10 0.0 6 10 103 10 0.0 6 10 104 4 0.0 6 4 105 11 0.0 6 11 106 5 0.0 6 5 107 6 0.0 6 6 108 8 0.0 6 8 109 34 0.0 6 33 0 0 0 1 110 9 0.0 6 9 111 12 0.0 6 12 112 11 0.0 6 11 113 10 0.0 6 10 114 19 0.0 6 19 115 3 0.0 6 3 116 7 0.0 6 7 117 6 0.0 6 6 118 6 0.0 6 5 0 0 0 1 119 5 0.0 6 5 120 7 0.0 6 7 121 7 0.0 6 7 122 4 0.0 6 4 123 10 0.0 6 10 124 15 0.0 6 15 125 8 0.0 6 8 126 9 0.0 6 9 127 8 0.0 6 7 0 0 1 128 5 0.0 6 5 129 4 0.0 6 4 130 12 0.0 6 12 131 6 0.0 6 6 132 12 0.0 6 12 133 21 0.0 6 21 134 7 0.0 6 7 135 6 0.0 6 6 136 11 0.0 6 11 137 7 0.0 6 6 0 0 0 1 138 8 0.0 6 8 139 4 0.0 6 4 140 10 0.0 6 10 141 6 0.0 6 6 142 8 0.0 6 8 143 7 0.0 6 7 144 2 0.0 6 1 0 0 0 1 145 5 0.0 6 5 146 3 0.0 6 3 147 11 0.0 6 11 148 5 0.0 6 5 149 8 0.0 6 8 150 5 0.0 6 5 151 10 0.0 6 10 152 6 0.0 6 6 153 3 0.0 6 3 154 6 0.0 6 6 155 5 0.0 6 5 156 5 0.0 6 5 157 20 0.0 6 20 158 10 0.0 6 10 159 10 0.0 6 10 160 5 0.0 6 5 161 7 0.0 6 7 162 15 0.0 6 15 163 11 0.0 6 11 164 8 0.0 6 8 165 7 0.0 6 7 166 3 0.0 6 3 167 10 0.0 6 10 168 6 0.0 6 6 169 6 0.0 6 6 170 7 0.0 6 7 171 5 0.0 6 5 172 5 0.0 6 5 173 9 0.0 6 9 174 3 0.0 6 3 175 3 0.0 6 3 176 8 0.0 6 7 0 0 0 1 177 5 0.0 6 5 178 7 0.0 6 7 179 4 0.0 6 4 180 4 0.0 6 4 181 3 0.0 6 3 182 5 0.0 6 5 183 8 0.0 6 8 184 11 0.0 6 11 185 1 0.0 6 1 186 4 0.0 6 3 0 0 0 1 187 7 0.0 6 7 188 5 0.0 6 5 189 7 0.0 6 7 190 9 0.0 6 9 191 2 0.0 6 2 192 1 0.0 6 1 193 2 0.0 6 2 194 1 0.0 6 1 195 1 0.0 6 1 196 6 0.0 6 6 197 2 0.0 6 2 199 2 0.0 6 2 200 4 0.0 6 4 201 5 0.0 6 5 202 1 0.0 6 1 203 6 0.0 6 6 204 1 0.0 6 1 205 1 0.0 6 1 206 1 0.0 6 1 207 1 0.0 6 1 209 1 0.0 6 1 211 3 0.0 6 3 213 1 0.0 6 1 214 1 0.0 6 1 217 1 0.0 6 1 222 2 0.0 6 2 234 1 0.0 6 1 239 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_09_222_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_09_449_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_09_222_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_09_449_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.76 s (63 µs/read; 0.96 M reads/minute). === Summary === Total read pairs processed: 91,927 Read 1 with adapter: 91,924 (100.0%) Read 2 with adapter: 91,891 (100.0%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 39 (0.0%) Pairs written (passing filters): 91,888 (100.0%) Total basepairs processed: 47,551,007 bp Read 1: 26,720,106 bp Read 2: 20,830,901 bp Total written (filtered): 5,698,815 bp (12.0%) Read 1: 3,033,102 bp Read 2: 2,665,713 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 91882 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 359.1 1 0 1 6 1 22.4 1 0 0 1 7 2 5.6 2 0 0 2 8 1 1.4 2 1 16 4 0.0 4 3 0 1 19 1 0.0 5 1 20 14 0.0 6 13 1 21 5 0.0 6 4 0 1 22 3 0.0 6 3 23 3 0.0 6 3 24 1 0.0 6 0 1 25 7 0.0 6 7 26 3 0.0 6 3 27 3 0.0 6 3 29 2 0.0 6 2 30 2 0.0 6 2 31 4 0.0 6 3 1 32 1 0.0 6 1 34 1 0.0 6 1 36 2 0.0 6 2 37 1 0.0 6 1 38 7 0.0 6 6 1 40 5 0.0 6 4 1 41 3 0.0 6 1 2 42 2 0.0 6 2 43 1 0.0 6 1 44 4 0.0 6 4 45 50 0.0 6 46 4 46 12 0.0 6 12 47 3 0.0 6 3 48 9 0.0 6 9 50 2 0.0 6 2 51 1 0.0 6 1 52 1 0.0 6 1 53 1 0.0 6 1 54 2 0.0 6 2 55 2 0.0 6 2 56 2 0.0 6 1 1 57 4 0.0 6 2 1 1 58 3 0.0 6 2 1 60 1 0.0 6 1 61 1 0.0 6 1 62 1 0.0 6 0 1 63 6 0.0 6 6 64 1 0.0 6 1 65 5 0.0 6 4 0 1 66 1 0.0 6 1 67 1 0.0 6 1 68 2 0.0 6 2 69 3 0.0 6 2 1 70 1 0.0 6 1 72 1 0.0 6 1 73 1 0.0 6 1 74 4 0.0 6 4 75 1 0.0 6 1 76 3 0.0 6 3 78 4 0.0 6 3 1 79 1 0.0 6 1 80 2 0.0 6 1 1 81 1 0.0 6 1 83 1 0.0 6 1 84 1 0.0 6 1 85 2 0.0 6 2 87 8 0.0 6 8 88 5 0.0 6 5 90 6 0.0 6 6 91 1 0.0 6 1 92 1 0.0 6 1 93 3 0.0 6 3 94 1 0.0 6 1 95 1 0.0 6 1 96 1 0.0 6 0 1 97 2 0.0 6 2 98 3 0.0 6 3 99 2 0.0 6 2 100 2 0.0 6 1 1 101 3 0.0 6 3 102 6 0.0 6 6 103 2 0.0 6 2 104 2 0.0 6 2 105 8 0.0 6 8 106 3 0.0 6 3 107 5 0.0 6 4 0 1 108 6 0.0 6 5 1 109 5 0.0 6 4 1 110 3 0.0 6 3 111 5 0.0 6 4 1 112 8 0.0 6 8 113 3 0.0 6 3 114 4 0.0 6 4 115 6 0.0 6 5 1 116 3 0.0 6 3 117 6 0.0 6 6 118 2 0.0 6 2 119 1 0.0 6 1 120 2 0.0 6 1 0 0 1 121 14 0.0 6 14 122 17 0.0 6 17 123 10 0.0 6 9 1 124 8 0.0 6 8 125 9 0.0 6 8 1 126 12 0.0 6 12 127 5 0.0 6 5 128 16 0.0 6 15 1 129 12 0.0 6 12 130 13 0.0 6 13 131 7 0.0 6 7 132 17 0.0 6 15 2 133 33 0.0 6 31 2 134 28 0.0 6 25 2 1 135 15 0.0 6 15 136 9 0.0 6 8 1 137 13 0.0 6 12 1 138 14 0.0 6 14 139 19 0.0 6 18 1 140 19 0.0 6 18 1 141 26 0.0 6 26 142 56 0.0 6 56 143 44 0.0 6 43 1 144 71 0.0 6 71 145 97 0.0 6 96 1 146 48 0.0 6 48 147 80 0.0 6 80 148 67 0.0 6 66 1 149 62 0.0 6 60 2 150 83 0.0 6 83 151 39 0.0 6 38 1 152 40 0.0 6 39 1 153 42 0.0 6 41 1 154 24 0.0 6 23 1 155 38 0.0 6 38 156 19 0.0 6 17 2 157 36 0.0 6 35 1 158 40 0.0 6 40 159 52 0.0 6 51 1 160 35 0.0 6 34 1 161 30 0.0 6 29 1 162 52 0.0 6 52 163 40 0.0 6 39 1 164 23 0.0 6 23 165 35 0.0 6 34 1 166 40 0.0 6 39 1 167 39 0.0 6 39 168 45 0.0 6 43 2 169 33 0.0 6 33 170 16 0.0 6 14 1 1 171 32 0.0 6 31 1 172 27 0.0 6 24 3 173 21 0.0 6 21 174 36 0.0 6 36 175 46 0.0 6 43 3 176 32 0.0 6 30 2 177 33 0.0 6 32 1 178 37 0.0 6 36 1 179 58 0.0 6 57 1 180 33 0.0 6 30 3 181 51 0.0 6 46 5 182 56 0.0 6 55 1 183 34 0.0 6 33 1 184 32 0.0 6 31 1 185 84 0.0 6 79 5 186 92 0.0 6 88 4 187 89 0.0 6 89 188 83 0.0 6 79 4 189 91 0.0 6 87 4 190 78 0.0 6 75 3 191 66 0.0 6 65 1 192 60 0.0 6 59 1 193 92 0.0 6 91 1 194 102 0.0 6 97 5 195 94 0.0 6 93 1 196 124 0.0 6 116 7 0 1 197 110 0.0 6 109 1 198 123 0.0 6 120 3 199 148 0.0 6 143 5 200 124 0.0 6 122 2 201 153 0.0 6 150 3 202 177 0.0 6 176 1 203 156 0.0 6 151 5 204 157 0.0 6 152 5 205 231 0.0 6 223 7 1 206 316 0.0 6 296 19 1 207 640 0.0 6 626 14 208 390 0.0 6 375 14 1 209 361 0.0 6 347 13 1 210 585 0.0 6 571 14 211 492 0.0 6 484 8 212 349 0.0 6 343 5 1 213 238 0.0 6 224 13 1 214 247 0.0 6 232 15 215 125 0.0 6 118 7 216 186 0.0 6 181 5 217 205 0.0 6 198 7 218 217 0.0 6 210 7 219 167 0.0 6 163 3 0 1 220 178 0.0 6 173 5 221 231 0.0 6 226 5 222 405 0.0 6 393 12 223 250 0.0 6 244 5 1 224 152 0.0 6 143 9 225 163 0.0 6 157 6 226 286 0.0 6 276 9 1 227 442 0.0 6 430 12 228 335 0.0 6 330 5 229 210 0.0 6 207 3 230 339 0.0 6 329 10 231 439 0.0 6 426 13 232 280 0.0 6 269 10 0 0 1 233 288 0.0 6 282 6 234 351 0.0 6 339 12 235 206 0.0 6 195 11 236 482 0.0 6 475 7 237 183 0.0 6 174 9 238 506 0.0 6 495 11 239 311 0.0 6 304 7 240 248 0.0 6 240 8 241 332 0.0 6 326 6 242 287 0.0 6 277 10 243 290 0.0 6 269 21 244 453 0.0 6 442 11 245 596 0.0 6 581 15 246 436 0.0 6 429 7 247 310 0.0 6 301 9 248 741 0.0 6 722 18 1 249 658 0.0 6 638 18 1 1 250 1826 0.0 6 1783 43 251 791 0.0 6 767 24 252 457 0.0 6 442 12 3 253 859 0.0 6 833 24 1 1 254 1097 0.0 6 1064 33 255 1751 0.0 6 1714 36 0 1 256 1577 0.0 6 1535 41 1 257 2542 0.0 6 2481 61 258 922 0.0 6 882 35 2 2 1 259 2159 0.0 6 2103 55 1 260 1242 0.0 6 1215 27 261 2304 0.0 6 2258 44 0 0 2 262 1142 0.0 6 1121 21 263 899 0.0 6 878 20 0 1 264 1430 0.0 6 1392 37 1 265 1840 0.0 6 1805 35 266 868 0.0 6 842 26 267 1742 0.0 6 1705 36 1 268 1677 0.0 6 1637 40 269 1123 0.0 6 1092 31 270 417 0.0 6 409 7 0 1 271 690 0.0 6 674 16 272 901 0.0 6 880 19 1 0 1 273 1327 0.0 6 1281 44 2 274 3884 0.0 6 3782 101 1 275 3287 0.0 6 3167 114 4 2 276 4704 0.0 6 4561 136 7 277 27713 0.0 6 27077 613 16 6 1 278 719 0.0 6 606 112 1 279 136 0.0 6 127 5 4 280 3 0.0 6 3 281 1 0.0 6 0 0 0 0 1 282 1 0.0 6 1 288 1 0.0 6 0 0 0 0 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 42 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 73 1 0.0 6 1 74 4 0.0 6 4 77 2 0.0 6 2 78 3 0.0 6 3 80 4 0.0 6 4 81 1 0.0 6 1 82 3 0.0 6 3 83 1 0.0 6 1 86 1 0.0 6 1 90 1 0.0 6 1 95 3 0.0 6 3 100 2 0.0 6 2 101 1 0.0 6 1 118 1 0.0 6 1 128 2 0.0 6 2 138 2 0.0 6 2 139 3 0.0 6 1 0 0 0 0 0 2 140 1 0.0 6 1 149 1 0.0 6 1 162 1 0.0 6 1 193 1 0.0 6 1 196 1 0.0 6 1 217 1 0.0 6 1 218 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 91864 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 7 2 5.6 2 0 2 11 1 0.0 3 1 12 2 0.0 3 2 13 6 0.0 3 4 2 14 16 0.0 4 16 15 1 0.0 4 0 0 1 16 2 0.0 4 1 1 17 10 0.0 5 9 1 18 2 0.0 5 2 19 17 0.0 5 15 2 20 45 0.0 6 45 21 40 0.0 6 38 1 0 1 22 31 0.0 6 30 1 23 15 0.0 6 15 24 10 0.0 6 10 25 14 0.0 6 13 1 26 13 0.0 6 13 27 10 0.0 6 9 1 28 8 0.0 6 8 29 11 0.0 6 9 2 30 11 0.0 6 10 0 1 31 5 0.0 6 4 1 32 7 0.0 6 7 33 14 0.0 6 11 2 1 34 7 0.0 6 6 1 35 6 0.0 6 5 1 36 5 0.0 6 5 37 8 0.0 6 8 38 7 0.0 6 6 1 39 13 0.0 6 12 1 40 14 0.0 6 12 2 41 7 0.0 6 7 42 2 0.0 6 1 1 43 10 0.0 6 8 2 44 12 0.0 6 10 2 45 8 0.0 6 8 46 16 0.0 6 14 2 47 10 0.0 6 10 48 13 0.0 6 11 2 49 20 0.0 6 20 50 19 0.0 6 17 1 1 51 14 0.0 6 14 52 21 0.0 6 19 2 53 8 0.0 6 8 54 5 0.0 6 5 55 19 0.0 6 19 56 33 0.0 6 27 3 3 57 25 0.0 6 20 5 58 21 0.0 6 20 0 1 59 12 0.0 6 10 2 60 16 0.0 6 14 2 61 17 0.0 6 16 1 62 9 0.0 6 9 63 19 0.0 6 18 1 64 11 0.0 6 9 2 65 11 0.0 6 11 66 17 0.0 6 13 3 1 67 10 0.0 6 9 1 68 30 0.0 6 26 3 1 69 32 0.0 6 26 3 3 70 191 0.0 6 186 5 71 33 0.0 6 29 4 72 100 0.0 6 98 2 73 22 0.0 6 20 1 0 0 1 74 50 0.0 6 45 5 75 29 0.0 6 29 76 20 0.0 6 20 77 35 0.0 6 33 2 78 45 0.0 6 43 2 79 29 0.0 6 26 3 80 27 0.0 6 26 1 81 28 0.0 6 28 82 32 0.0 6 32 83 23 0.0 6 22 0 1 84 51 0.0 6 47 3 1 85 16 0.0 6 16 86 42 0.0 6 41 1 87 20 0.0 6 19 0 0 0 1 88 15 0.0 6 14 1 89 11 0.0 6 11 90 24 0.0 6 23 0 1 91 18 0.0 6 18 92 24 0.0 6 24 93 36 0.0 6 36 94 56 0.0 6 54 1 0 0 0 1 95 22 0.0 6 20 2 96 38 0.0 6 33 4 0 0 0 1 97 81 0.0 6 77 4 98 30 0.0 6 28 2 99 30 0.0 6 25 4 0 0 1 100 21 0.0 6 20 1 101 40 0.0 6 33 6 1 102 49 0.0 6 46 3 103 71 0.0 6 70 1 104 67 0.0 6 62 4 1 105 83 0.0 6 78 3 1 0 1 106 35 0.0 6 34 1 107 68 0.0 6 66 2 108 36 0.0 6 34 2 109 19 0.0 6 17 2 110 33 0.0 6 31 2 111 26 0.0 6 23 3 112 15 0.0 6 13 0 1 0 1 113 25 0.0 6 22 2 0 0 1 114 16 0.0 6 15 1 115 46 0.0 6 42 4 116 68 0.0 6 65 3 117 41 0.0 6 39 2 118 32 0.0 6 30 2 119 63 0.0 6 59 3 1 120 47 0.0 6 45 2 121 62 0.0 6 56 5 1 122 66 0.0 6 60 6 123 126 0.0 6 117 7 2 124 77 0.0 6 71 5 1 125 73 0.0 6 71 0 2 126 123 0.0 6 120 3 127 139 0.0 6 134 4 0 0 1 128 77 0.0 6 74 3 129 140 0.0 6 132 7 1 130 119 0.0 6 107 9 1 0 2 131 152 0.0 6 147 5 132 76 0.0 6 68 6 1 1 133 78 0.0 6 76 1 1 134 120 0.0 6 118 1 0 0 1 135 182 0.0 6 168 12 2 136 175 0.0 6 167 8 137 128 0.0 6 125 3 138 259 0.0 6 247 11 1 139 163 0.0 6 160 3 140 139 0.0 6 131 8 141 246 0.0 6 239 6 1 142 190 0.0 6 185 5 143 354 0.0 6 341 12 0 0 1 144 261 0.0 6 255 6 145 174 0.0 6 167 7 146 288 0.0 6 269 18 1 147 197 0.0 6 193 4 148 336 0.0 6 323 13 149 263 0.0 6 257 5 0 0 1 150 356 0.0 6 341 14 1 151 318 0.0 6 313 5 152 430 0.0 6 415 14 1 153 369 0.0 6 352 17 154 466 0.0 6 449 14 2 0 0 0 1 155 781 0.0 6 750 29 1 1 156 1001 0.0 6 966 34 1 157 601 0.0 6 586 12 2 0 1 158 337 0.0 6 321 14 1 0 1 159 420 0.0 6 410 10 160 308 0.0 6 294 13 0 1 161 380 0.0 6 366 11 1 2 162 358 0.0 6 345 9 4 163 587 0.0 6 564 22 1 164 1189 0.0 6 1157 32 165 454 0.0 6 435 18 0 0 1 166 772 0.0 6 756 16 167 408 0.0 6 395 12 0 1 168 329 0.0 6 318 11 169 569 0.0 6 547 20 1 0 1 170 582 0.0 6 573 9 171 764 0.0 6 737 24 1 1 1 172 1148 0.0 6 1105 42 0 0 1 173 367 0.0 6 349 18 174 1138 0.0 6 1101 36 0 0 1 175 1093 0.0 6 1066 27 176 398 0.0 6 383 14 0 1 177 431 0.0 6 419 12 178 396 0.0 6 387 6 2 0 1 179 696 0.0 6 673 21 2 180 295 0.0 6 288 6 0 0 1 181 510 0.0 6 498 11 1 182 312 0.0 6 301 10 0 1 183 769 0.0 6 747 21 1 184 323 0.0 6 302 19 1 1 185 779 0.0 6 764 13 2 186 442 0.0 6 426 13 0 1 2 187 1102 0.0 6 1074 26 2 188 1094 0.0 6 1062 30 1 0 1 189 1316 0.0 6 1288 25 2 1 190 745 0.0 6 726 18 1 191 881 0.0 6 852 27 2 192 1227 0.0 6 1201 21 4 1 193 846 0.0 6 817 26 2 0 1 194 1414 0.0 6 1381 29 4 195 781 0.0 6 762 16 2 1 196 1128 0.0 6 1093 27 3 1 4 197 497 0.0 6 471 20 4 1 1 198 1238 0.0 6 1193 43 1 0 1 199 1968 0.0 6 1923 45 200 1717 0.0 6 1674 40 1 0 2 201 1094 0.0 6 1047 40 2 2 2 1 202 1057 0.0 6 1027 29 1 203 1012 0.0 6 981 30 0 0 1 204 694 0.0 6 679 15 205 668 0.0 6 651 16 1 206 1742 0.0 6 1703 37 2 207 381 0.0 6 367 12 2 208 664 0.0 6 644 18 0 0 2 209 940 0.0 6 918 21 0 0 1 210 628 0.0 6 611 17 211 266 0.0 6 256 10 212 749 0.0 6 733 15 0 0 0 1 213 175 0.0 6 170 4 0 0 1 214 369 0.0 6 356 11 0 1 0 0 1 215 302 0.0 6 291 10 1 216 759 0.0 6 747 11 1 217 526 0.0 6 512 11 3 218 702 0.0 6 676 25 1 219 1002 0.0 6 960 41 1 220 375 0.0 6 369 5 0 1 221 410 0.0 6 393 16 1 222 533 0.0 6 511 20 0 1 1 223 1716 0.0 6 1652 62 1 0 1 224 4585 0.0 6 4478 104 1 2 225 1951 0.0 6 1896 50 3 0 2 226 1593 0.0 6 1502 88 2 1 227 6772 0.0 6 6620 148 2 0 2 228 843 0.0 6 814 28 0 0 1 229 2717 0.0 6 2655 60 1 0 0 1 230 410 0.0 6 388 18 2 2 231 1169 0.0 6 1135 31 0 2 1 232 1148 0.0 6 1116 31 1 233 1322 0.0 6 1275 45 1 1 234 1760 0.0 6 1727 33 235 761 0.0 6 741 18 0 2 236 89 0.0 6 86 2 1 237 172 0.0 6 170 2 238 275 0.0 6 265 10 239 75 0.0 6 72 2 1 240 35 0.0 6 33 2 241 295 0.0 6 291 4 242 105 0.0 6 100 3 0 0 1 1 243 190 0.0 6 180 10 244 975 0.0 6 954 19 1 0 1 245 77 0.0 6 71 6 246 140 0.0 6 134 5 1 247 85 0.0 6 83 2 248 181 0.0 6 174 7 249 236 0.0 6 228 8 250 85 0.0 6 83 1 1 251 604 0.0 6 589 14 1 252 344 0.0 6 339 5 253 184 0.0 6 177 7 254 388 0.0 6 380 6 0 0 2 255 45 0.0 6 44 1 256 39 0.0 6 38 1 257 10 0.0 6 10 258 3 0.0 6 2 0 1 262 1 0.0 6 1 269 1 0.0 6 1 275 1 0.0 6 1 278 1 0.0 6 1 279 13 0.0 6 13 280 17 0.0 6 17 281 162 0.0 6 157 5 282 749 0.0 6 732 17 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 27 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 73 2 0.0 6 2 74 1 0.0 6 1 75 1 0.0 6 1 76 1 0.0 6 1 77 2 0.0 6 2 81 1 0.0 6 1 82 1 0.0 6 1 84 1 0.0 6 1 85 1 0.0 6 1 86 2 0.0 6 2 89 1 0.0 6 1 90 1 0.0 6 1 92 1 0.0 6 1 93 1 0.0 6 1 99 1 0.0 6 1 101 1 0.0 6 1 102 1 0.0 6 1 104 1 0.0 6 1 106 1 0.0 6 1 107 1 0.0 6 1 108 1 0.0 6 1 134 1 0.0 6 1 160 1 0.0 6 1 189 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_10_223_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_10_450_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_10_223_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_10_450_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 6.71 s (84 µs/read; 0.72 M reads/minute). === Summary === Total read pairs processed: 80,073 Read 1 with adapter: 80,064 (100.0%) Read 2 with adapter: 80,047 (100.0%) == Read fate breakdown == Pairs that were too short: 75 (0.1%) Pairs discarded as untrimmed: 35 (0.0%) Pairs written (passing filters): 79,963 (99.9%) Total basepairs processed: 33,679,890 bp Read 1: 18,693,756 bp Read 2: 14,986,134 bp Total written (filtered): 5,613,420 bp (16.7%) Read 1: 2,986,019 bp Read 2: 2,627,401 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 72937 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 1251.1 0 0 1 4 3 312.8 1 3 6 6 19.5 1 1 0 5 7 2 4.9 2 0 1 1 8 2 1.2 2 2 9 2 0.3 2 2 10 1 0.1 3 1 14 1 0.0 4 1 15 1 0.0 4 1 16 1 0.0 4 1 17 1 0.0 5 1 18 1 0.0 5 1 19 4 0.0 5 3 1 20 101 0.0 6 90 11 21 42 0.0 6 35 7 22 61 0.0 6 54 7 23 24 0.0 6 21 3 24 19 0.0 6 17 2 25 15 0.0 6 14 1 26 17 0.0 6 13 2 1 0 0 1 27 12 0.0 6 11 1 28 9 0.0 6 9 29 9 0.0 6 9 30 6 0.0 6 5 1 31 7 0.0 6 7 32 13 0.0 6 10 3 33 8 0.0 6 6 0 1 0 0 0 1 34 10 0.0 6 7 2 0 1 35 5 0.0 6 5 36 9 0.0 6 8 1 37 6 0.0 6 5 1 38 8 0.0 6 6 2 39 11 0.0 6 10 1 40 8 0.0 6 7 1 41 6 0.0 6 6 42 4 0.0 6 4 43 4 0.0 6 4 44 5 0.0 6 3 2 45 7 0.0 6 5 1 1 46 7 0.0 6 6 1 47 9 0.0 6 7 2 48 6 0.0 6 6 49 7 0.0 6 6 0 1 50 9 0.0 6 9 51 3 0.0 6 3 52 6 0.0 6 5 1 53 4 0.0 6 3 0 0 0 0 1 54 10 0.0 6 8 1 1 55 3 0.0 6 2 1 56 2 0.0 6 2 57 5 0.0 6 5 58 4 0.0 6 3 0 1 59 5 0.0 6 4 1 60 7 0.0 6 6 0 0 0 0 0 1 61 10 0.0 6 9 1 62 4 0.0 6 4 63 3 0.0 6 3 64 13 0.0 6 11 2 65 8 0.0 6 7 1 66 9 0.0 6 8 1 67 6 0.0 6 6 68 9 0.0 6 6 3 69 14 0.0 6 14 70 13 0.0 6 12 1 71 15 0.0 6 14 1 72 7 0.0 6 7 73 8 0.0 6 8 74 14 0.0 6 10 3 0 1 75 15 0.0 6 15 76 9 0.0 6 9 77 8 0.0 6 7 0 0 1 78 10 0.0 6 9 1 79 13 0.0 6 9 3 1 80 14 0.0 6 11 2 0 0 0 0 1 81 14 0.0 6 14 82 12 0.0 6 11 1 83 18 0.0 6 16 2 84 12 0.0 6 11 0 1 85 8 0.0 6 7 1 86 9 0.0 6 8 1 87 8 0.0 6 7 1 88 8 0.0 6 7 1 89 14 0.0 6 14 90 5 0.0 6 4 1 91 12 0.0 6 11 1 92 7 0.0 6 7 93 21 0.0 6 20 1 94 8 0.0 6 7 1 95 16 0.0 6 15 1 96 8 0.0 6 8 97 12 0.0 6 11 1 98 9 0.0 6 7 2 99 7 0.0 6 5 2 100 17 0.0 6 15 2 101 17 0.0 6 13 4 102 14 0.0 6 12 2 103 27 0.0 6 23 4 104 30 0.0 6 26 4 105 17 0.0 6 15 2 106 11 0.0 6 11 107 13 0.0 6 13 108 19 0.0 6 19 109 18 0.0 6 16 1 0 0 0 0 1 110 32 0.0 6 32 111 26 0.0 6 23 3 112 23 0.0 6 21 2 113 24 0.0 6 22 2 114 32 0.0 6 32 115 29 0.0 6 29 116 31 0.0 6 27 4 117 30 0.0 6 26 4 118 44 0.0 6 40 4 119 27 0.0 6 26 1 120 35 0.0 6 33 0 0 1 0 1 121 28 0.0 6 28 122 24 0.0 6 23 1 123 30 0.0 6 30 124 41 0.0 6 37 2 0 0 1 0 1 125 36 0.0 6 35 0 1 126 29 0.0 6 28 1 127 41 0.0 6 41 128 44 0.0 6 43 1 129 59 0.0 6 52 6 1 130 61 0.0 6 58 3 131 37 0.0 6 36 1 132 37 0.0 6 36 1 133 44 0.0 6 43 1 134 39 0.0 6 38 1 135 47 0.0 6 43 4 136 47 0.0 6 47 137 41 0.0 6 40 1 138 52 0.0 6 51 1 139 76 0.0 6 75 1 140 79 0.0 6 76 3 141 91 0.0 6 90 1 142 86 0.0 6 84 2 143 223 0.0 6 219 4 144 445 0.0 6 443 2 145 383 0.0 6 378 5 146 455 0.0 6 450 5 147 295 0.0 6 291 4 148 297 0.0 6 295 2 149 419 0.0 6 417 2 150 1029 0.0 6 1018 9 1 0 0 0 1 151 654 0.0 6 650 3 1 152 825 0.0 6 814 10 0 0 0 1 153 658 0.0 6 650 7 1 154 223 0.0 6 214 9 155 235 0.0 6 231 4 156 754 0.0 6 745 9 157 832 0.0 6 827 5 158 660 0.0 6 652 8 159 334 0.0 6 332 2 160 238 0.0 6 237 1 161 566 0.0 6 557 9 162 824 0.0 6 811 11 1 1 163 895 0.0 6 887 8 164 475 0.0 6 467 8 165 581 0.0 6 573 6 0 0 0 0 2 166 313 0.0 6 305 7 1 167 267 0.0 6 260 6 0 0 0 1 168 735 0.0 6 730 5 169 808 0.0 6 801 7 170 284 0.0 6 278 6 171 163 0.0 6 161 2 172 217 0.0 6 211 6 173 224 0.0 6 217 7 174 382 0.0 6 370 12 175 411 0.0 6 408 2 0 1 176 554 0.0 6 546 8 177 358 0.0 6 347 11 178 265 0.0 6 257 8 179 279 0.0 6 272 5 2 180 302 0.0 6 293 9 181 468 0.0 6 462 6 182 375 0.0 6 372 3 183 388 0.0 6 381 7 184 414 0.0 6 404 9 1 185 274 0.0 6 264 10 186 290 0.0 6 283 7 187 411 0.0 6 405 5 0 0 1 188 399 0.0 6 390 7 2 189 465 0.0 6 457 8 190 384 0.0 6 377 7 191 472 0.0 6 459 13 192 578 0.0 6 560 17 0 0 1 193 1791 0.0 6 1777 14 194 848 0.0 6 836 11 0 0 0 0 1 195 1107 0.0 6 1085 21 0 0 0 0 1 196 1082 0.0 6 1065 16 1 197 1052 0.0 6 1030 19 1 0 0 0 2 198 668 0.0 6 651 13 0 0 0 0 4 199 527 0.0 6 512 13 0 1 1 200 648 0.0 6 634 13 0 1 201 729 0.0 6 712 14 3 202 1025 0.0 6 1006 17 0 0 0 0 2 203 603 0.0 6 591 9 1 0 1 0 1 204 845 0.0 6 825 19 0 0 0 0 1 205 1118 0.0 6 1087 29 0 0 1 0 1 206 1079 0.0 6 1059 19 0 1 207 1357 0.0 6 1331 26 208 1098 0.0 6 1083 15 209 550 0.0 6 537 13 210 829 0.0 6 807 22 211 1275 0.0 6 1254 21 212 800 0.0 6 784 13 0 1 0 0 2 213 840 0.0 6 812 24 1 1 0 1 1 214 1028 0.0 6 990 33 0 1 0 0 4 215 827 0.0 6 808 18 1 216 1176 0.0 6 1157 18 0 0 0 1 217 901 0.0 6 878 23 218 855 0.0 6 837 17 0 1 219 728 0.0 6 720 8 220 891 0.0 6 873 16 0 0 1 1 221 700 0.0 6 692 8 222 754 0.0 6 731 21 2 223 611 0.0 6 601 10 224 749 0.0 6 737 12 225 655 0.0 6 642 13 226 595 0.0 6 590 4 0 0 0 1 227 601 0.0 6 593 8 228 648 0.0 6 638 10 229 620 0.0 6 611 8 1 230 513 0.0 6 506 7 231 631 0.0 6 620 11 232 724 0.0 6 713 9 0 0 1 1 233 585 0.0 6 571 14 234 529 0.0 6 522 7 235 521 0.0 6 516 4 1 236 470 0.0 6 456 13 1 237 524 0.0 6 513 10 1 238 498 0.0 6 493 4 0 0 0 1 239 506 0.0 6 497 8 1 240 615 0.0 6 609 5 0 0 0 1 241 539 0.0 6 535 3 0 0 0 0 1 242 337 0.0 6 335 2 243 312 0.0 6 305 7 244 321 0.0 6 316 5 245 230 0.0 6 227 3 246 217 0.0 6 211 6 247 233 0.0 6 228 5 248 255 0.0 6 254 1 249 261 0.0 6 254 5 1 1 250 285 0.0 6 282 3 251 209 0.0 6 208 1 252 229 0.0 6 224 5 253 159 0.0 6 158 1 254 190 0.0 6 187 3 255 192 0.0 6 188 4 256 167 0.0 6 164 3 257 191 0.0 6 188 3 258 261 0.0 6 253 8 259 161 0.0 6 159 2 260 131 0.0 6 128 3 261 145 0.0 6 144 1 262 149 0.0 6 148 1 263 134 0.0 6 129 4 0 0 0 0 1 264 162 0.0 6 160 2 265 104 0.0 6 103 1 266 161 0.0 6 157 3 0 0 0 0 1 267 137 0.0 6 136 1 268 130 0.0 6 127 3 269 107 0.0 6 103 2 0 0 0 0 2 270 102 0.0 6 95 6 0 0 0 0 1 271 67 0.0 6 66 1 272 82 0.0 6 81 1 273 118 0.0 6 113 4 1 274 146 0.0 6 143 3 275 168 0.0 6 163 5 276 237 0.0 6 229 6 2 277 1076 0.0 6 1047 28 0 0 0 0 1 278 1167 0.0 6 1136 29 2 279 22 0.0 6 22 280 2 0.0 6 2 283 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 7127 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 63 1 0.0 6 1 64 133 0.0 6 129 4 65 1 0.0 6 1 72 3 0.0 6 3 73 21 0.0 6 21 74 58 0.0 6 58 75 37 0.0 6 37 76 48 0.0 6 48 77 19 0.0 6 19 78 27 0.0 6 27 79 51 0.0 6 51 80 136 0.0 6 136 81 73 0.0 6 73 82 145 0.0 6 144 1 83 74 0.0 6 74 84 7 0.0 6 7 85 19 0.0 6 18 1 86 79 0.0 6 78 1 87 101 0.0 6 101 88 95 0.0 6 95 89 17 0.0 6 17 90 15 0.0 6 15 91 75 0.0 6 75 92 101 0.0 6 101 93 100 0.0 6 100 94 35 0.0 6 35 95 71 0.0 6 70 0 0 0 0 0 1 96 13 0.0 6 13 97 22 0.0 6 22 98 84 0.0 6 83 1 99 98 0.0 6 97 1 100 27 0.0 6 27 101 1 0.0 6 1 102 3 0.0 6 3 103 11 0.0 6 11 104 35 0.0 6 34 0 0 0 0 0 1 105 18 0.0 6 18 106 58 0.0 6 58 107 34 0.0 6 34 108 12 0.0 6 12 109 24 0.0 6 24 110 22 0.0 6 22 111 49 0.0 6 49 112 18 0.0 6 18 113 28 0.0 6 28 114 38 0.0 6 38 115 8 0.0 6 8 116 3 0.0 6 3 117 28 0.0 6 28 118 21 0.0 6 20 1 119 23 0.0 6 23 120 8 0.0 6 8 121 10 0.0 6 10 122 51 0.0 6 51 123 289 0.0 6 289 124 25 0.0 6 25 125 84 0.0 6 84 126 78 0.0 6 78 127 95 0.0 6 95 128 21 0.0 6 21 129 19 0.0 6 19 130 17 0.0 6 17 131 25 0.0 6 25 132 111 0.0 6 111 133 16 0.0 6 16 134 28 0.0 6 28 135 32 0.0 6 32 136 46 0.0 6 46 137 55 0.0 6 55 138 178 0.0 6 178 139 34 0.0 6 34 140 22 0.0 6 22 141 146 0.0 6 146 142 1 0.0 6 0 1 143 2 0.0 6 2 144 34 0.0 6 32 0 0 0 0 0 2 145 17 0.0 6 17 146 151 0.0 6 151 147 3 0.0 6 3 148 2 0.0 6 2 149 11 0.0 6 11 150 51 0.0 6 51 151 54 0.0 6 54 152 94 0.0 6 92 2 153 10 0.0 6 10 154 62 0.0 6 62 155 7 0.0 6 7 156 16 0.0 6 16 157 50 0.0 6 50 158 129 0.0 6 128 1 159 43 0.0 6 43 160 2 0.0 6 2 161 4 0.0 6 4 162 16 0.0 6 16 163 22 0.0 6 22 164 7 0.0 6 7 165 7 0.0 6 7 166 14 0.0 6 14 167 85 0.0 6 85 168 58 0.0 6 58 169 111 0.0 6 110 1 170 190 0.0 6 190 171 132 0.0 6 132 172 2 0.0 6 2 173 22 0.0 6 21 0 0 0 0 0 1 174 73 0.0 6 73 175 62 0.0 6 62 176 8 0.0 6 8 177 27 0.0 6 27 178 4 0.0 6 4 179 5 0.0 6 5 180 29 0.0 6 29 181 72 0.0 6 72 182 36 0.0 6 36 183 117 0.0 6 117 184 43 0.0 6 43 185 16 0.0 6 16 186 27 0.0 6 26 1 187 10 0.0 6 10 188 7 0.0 6 7 189 27 0.0 6 27 190 1 0.0 6 1 191 2 0.0 6 2 192 14 0.0 6 14 193 24 0.0 6 24 194 29 0.0 6 29 195 36 0.0 6 36 196 53 0.0 6 52 1 197 3 0.0 6 3 198 22 0.0 6 22 199 14 0.0 6 14 200 7 0.0 6 7 201 32 0.0 6 32 202 2 0.0 6 2 203 19 0.0 6 19 204 52 0.0 6 52 205 20 0.0 6 19 1 206 13 0.0 6 13 207 15 0.0 6 15 208 24 0.0 6 24 209 6 0.0 6 6 210 5 0.0 6 5 211 2 0.0 6 2 212 13 0.0 6 13 213 94 0.0 6 94 214 17 0.0 6 16 1 215 16 0.0 6 16 216 12 0.0 6 12 217 9 0.0 6 9 218 79 0.0 6 79 219 9 0.0 6 9 220 3 0.0 6 3 221 8 0.0 6 8 222 1 0.0 6 1 223 3 0.0 6 3 224 15 0.0 6 15 225 8 0.0 6 8 226 10 0.0 6 10 227 57 0.0 6 57 228 12 0.0 6 12 229 17 0.0 6 17 230 25 0.0 6 25 231 18 0.0 6 18 232 20 0.0 6 20 233 120 0.0 6 120 234 8 0.0 6 8 235 2 0.0 6 2 236 6 0.0 6 6 237 1 0.0 6 1 238 6 0.0 6 6 239 12 0.0 6 12 240 2 0.0 6 2 241 3 0.0 6 3 242 9 0.0 6 9 244 22 0.0 6 22 245 1 0.0 6 1 246 20 0.0 6 20 248 2 0.0 6 2 249 15 0.0 6 15 250 42 0.0 6 42 251 28 0.0 6 28 252 196 0.0 6 196 253 12 0.0 6 12 254 4 0.0 6 4 255 10 0.0 6 10 256 2 0.0 6 2 257 28 0.0 6 28 258 35 0.0 6 35 259 13 0.0 6 13 262 3 0.0 6 3 264 3 0.0 6 3 265 1 0.0 6 1 266 2 0.0 6 2 267 9 0.0 6 9 268 9 0.0 6 9 269 1 0.0 6 1 270 10 0.0 6 10 271 5 0.0 6 5 272 6 0.0 6 6 273 5 0.0 6 4 1 274 4 0.0 6 4 277 6 0.0 6 6 278 1 0.0 6 1 279 1 0.0 6 1 280 18 0.0 6 18 281 1 0.0 6 1 282 2 0.0 6 2 291 1 0.0 6 1 292 2 0.0 6 2 294 6 0.0 6 6 295 2 0.0 6 2 296 1 0.0 6 1 297 4 0.0 6 4 298 1 0.0 6 1 302 1 0.0 6 1 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 77793 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 4 1251.1 0 0 4 5 1 78.2 1 1 7 1 4.9 2 1 9 2 0.3 2 1 1 10 2 0.1 3 2 11 3 0.0 3 2 0 1 12 2 0.0 3 2 13 11 0.0 3 8 3 14 12 0.0 4 12 15 2 0.0 4 1 1 16 23 0.0 4 19 3 1 17 25 0.0 5 20 5 18 23 0.0 5 21 2 19 22 0.0 5 20 2 20 104 0.0 6 96 8 21 60 0.0 6 57 3 22 43 0.0 6 42 1 23 39 0.0 6 39 24 25 0.0 6 24 1 25 30 0.0 6 28 1 0 0 0 1 26 24 0.0 6 22 2 27 18 0.0 6 17 1 28 25 0.0 6 25 29 18 0.0 6 18 30 23 0.0 6 19 4 31 25 0.0 6 23 2 32 14 0.0 6 13 0 0 0 0 0 1 33 20 0.0 6 15 4 0 0 0 0 1 34 20 0.0 6 18 2 35 13 0.0 6 13 36 15 0.0 6 15 37 25 0.0 6 23 2 38 11 0.0 6 9 1 1 39 17 0.0 6 15 2 40 20 0.0 6 19 1 41 15 0.0 6 15 42 18 0.0 6 18 43 13 0.0 6 10 2 0 0 0 0 1 44 18 0.0 6 17 1 45 16 0.0 6 16 46 18 0.0 6 14 4 47 16 0.0 6 14 2 48 20 0.0 6 15 3 2 49 22 0.0 6 21 1 50 22 0.0 6 19 3 51 30 0.0 6 27 2 1 52 37 0.0 6 33 3 0 0 1 53 28 0.0 6 26 1 0 0 1 54 28 0.0 6 25 2 0 1 55 35 0.0 6 31 2 1 0 0 1 56 22 0.0 6 19 3 57 26 0.0 6 24 2 58 35 0.0 6 28 7 59 35 0.0 6 34 1 60 37 0.0 6 34 3 61 38 0.0 6 34 1 1 1 1 62 31 0.0 6 30 1 63 45 0.0 6 38 5 1 0 1 64 44 0.0 6 40 3 1 65 34 0.0 6 34 66 58 0.0 6 56 2 67 160 0.0 6 155 5 68 70 0.0 6 67 2 0 0 0 1 69 78 0.0 6 76 2 70 53 0.0 6 47 4 2 71 52 0.0 6 50 2 72 42 0.0 6 40 2 73 28 0.0 6 25 3 74 50 0.0 6 43 7 75 42 0.0 6 38 4 76 44 0.0 6 42 2 77 49 0.0 6 43 3 3 78 34 0.0 6 33 1 79 70 0.0 6 66 4 80 67 0.0 6 61 5 0 0 1 81 79 0.0 6 72 6 0 0 1 82 86 0.0 6 83 3 83 81 0.0 6 77 3 0 0 0 1 84 64 0.0 6 63 1 85 69 0.0 6 66 3 86 56 0.0 6 52 4 87 56 0.0 6 52 4 88 70 0.0 6 62 7 0 0 1 89 67 0.0 6 64 2 1 90 73 0.0 6 67 3 1 0 2 91 99 0.0 6 87 11 1 92 92 0.0 6 83 7 1 0 0 0 1 93 97 0.0 6 91 5 1 94 83 0.0 6 77 5 1 95 124 0.0 6 118 3 1 1 0 0 1 96 121 0.0 6 114 5 0 0 1 0 1 97 105 0.0 6 102 3 98 113 0.0 6 105 7 1 99 76 0.0 6 70 6 100 108 0.0 6 102 6 101 110 0.0 6 107 2 1 102 126 0.0 6 120 6 103 132 0.0 6 129 2 0 1 104 201 0.0 6 194 6 1 105 128 0.0 6 121 6 1 106 138 0.0 6 134 4 107 148 0.0 6 141 4 0 1 2 108 146 0.0 6 144 2 109 137 0.0 6 134 3 110 159 0.0 6 150 6 0 0 1 0 2 111 161 0.0 6 157 3 0 1 112 197 0.0 6 192 4 0 0 0 0 1 113 183 0.0 6 178 4 0 1 114 159 0.0 6 150 9 115 215 0.0 6 206 9 116 190 0.0 6 185 4 0 0 1 117 212 0.0 6 202 9 1 118 232 0.0 6 226 4 0 0 2 119 215 0.0 6 212 3 120 208 0.0 6 205 3 121 241 0.0 6 234 6 0 0 0 1 122 237 0.0 6 230 5 0 0 1 1 123 271 0.0 6 263 7 1 124 319 0.0 6 310 8 1 125 338 0.0 6 321 15 2 126 347 0.0 6 339 6 0 1 0 0 1 127 376 0.0 6 366 7 1 0 2 128 399 0.0 6 388 9 1 0 0 0 1 129 422 0.0 6 411 7 1 1 1 1 130 469 0.0 6 457 11 0 1 131 538 0.0 6 528 9 0 0 1 132 561 0.0 6 539 16 4 0 2 133 626 0.0 6 607 16 0 1 1 0 1 134 628 0.0 6 611 13 2 0 0 2 135 570 0.0 6 549 14 4 1 0 0 2 136 524 0.0 6 508 13 2 0 0 1 137 637 0.0 6 625 9 2 0 1 138 596 0.0 6 583 13 139 648 0.0 6 633 14 0 0 0 0 1 140 772 0.0 6 754 17 0 0 0 1 141 907 0.0 6 885 19 0 0 2 1 142 1153 0.0 6 1136 16 0 0 1 143 971 0.0 6 952 17 0 0 1 0 1 144 1212 0.0 6 1196 14 2 145 1037 0.0 6 1013 19 2 3 146 1168 0.0 6 1148 20 147 1213 0.0 6 1189 18 3 1 1 0 1 148 1964 0.0 6 1936 26 0 1 1 149 1564 0.0 6 1546 15 2 0 0 0 1 150 2280 0.0 6 2240 33 6 0 1 151 1522 0.0 6 1507 14 1 152 1415 0.0 6 1395 18 0 0 1 0 1 153 1542 0.0 6 1517 24 0 0 1 154 1473 0.0 6 1449 19 3 1 0 1 155 1633 0.0 6 1617 13 1 1 1 156 1323 0.0 6 1303 16 1 0 0 2 1 157 1292 0.0 6 1278 12 0 0 0 0 2 158 1322 0.0 6 1301 18 0 0 1 1 1 159 1199 0.0 6 1182 16 0 0 1 160 1404 0.0 6 1386 16 2 161 1203 0.0 6 1185 14 2 2 162 920 0.0 6 906 12 0 0 1 1 163 1055 0.0 6 1042 10 0 0 2 0 1 164 937 0.0 6 927 10 165 923 0.0 6 909 11 3 166 1349 0.0 6 1334 11 0 1 3 167 1050 0.0 6 1037 11 0 0 1 1 168 1094 0.0 6 1082 10 0 0 0 1 1 169 1013 0.0 6 1001 10 1 0 0 1 170 944 0.0 6 932 11 0 0 0 1 171 1110 0.0 6 1100 9 0 1 172 1112 0.0 6 1100 12 173 939 0.0 6 928 9 1 1 174 1006 0.0 6 997 8 0 0 0 1 175 870 0.0 6 861 8 1 176 1023 0.0 6 1011 11 0 0 0 1 177 795 0.0 6 785 8 0 0 0 2 178 850 0.0 6 839 11 179 625 0.0 6 617 7 0 0 0 1 180 683 0.0 6 677 6 181 518 0.0 6 514 4 182 526 0.0 6 521 4 0 0 0 0 1 183 890 0.0 6 881 9 184 1073 0.0 6 1055 16 0 1 1 185 939 0.0 6 924 11 3 0 0 1 186 689 0.0 6 681 7 0 0 0 1 187 711 0.0 6 700 10 0 1 188 700 0.0 6 690 9 0 1 189 579 0.0 6 577 2 190 546 0.0 6 540 5 0 0 0 1 191 611 0.0 6 606 3 1 1 192 462 0.0 6 452 10 193 418 0.0 6 408 6 1 0 1 2 194 400 0.0 6 393 6 0 0 0 1 195 297 0.0 6 294 3 196 378 0.0 6 374 4 197 302 0.0 6 299 3 198 278 0.0 6 273 4 0 0 0 1 199 229 0.0 6 226 3 200 207 0.0 6 204 3 201 168 0.0 6 166 2 202 137 0.0 6 135 2 203 147 0.0 6 147 204 165 0.0 6 165 205 156 0.0 6 155 1 206 162 0.0 6 160 2 207 131 0.0 6 129 2 208 164 0.0 6 163 1 209 149 0.0 6 147 2 210 71 0.0 6 71 211 86 0.0 6 85 1 212 158 0.0 6 156 1 0 0 0 0 1 213 138 0.0 6 136 2 214 202 0.0 6 198 2 0 0 0 2 215 80 0.0 6 78 1 0 0 0 1 216 74 0.0 6 73 1 217 79 0.0 6 77 2 218 62 0.0 6 61 0 0 0 0 1 219 60 0.0 6 58 2 220 22 0.0 6 21 1 221 58 0.0 6 58 222 68 0.0 6 65 0 2 0 0 1 223 36 0.0 6 35 1 224 42 0.0 6 42 225 25 0.0 6 24 1 226 25 0.0 6 25 227 32 0.0 6 32 228 24 0.0 6 24 229 27 0.0 6 27 230 30 0.0 6 30 231 14 0.0 6 13 1 232 21 0.0 6 21 233 14 0.0 6 14 234 13 0.0 6 13 235 11 0.0 6 11 236 9 0.0 6 9 237 3 0.0 6 3 238 14 0.0 6 14 239 7 0.0 6 7 241 7 0.0 6 7 242 12 0.0 6 12 243 6 0.0 6 6 244 3 0.0 6 3 245 7 0.0 6 7 246 5 0.0 6 5 247 3 0.0 6 3 248 4 0.0 6 3 1 249 1 0.0 6 1 250 1 0.0 6 1 251 7 0.0 6 6 1 252 1 0.0 6 1 254 1 0.0 6 1 276 1 0.0 6 1 277 2 0.0 6 2 278 6 0.0 6 5 1 279 54 0.0 6 53 1 280 3 0.0 6 3 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 2254 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 49 1 0.0 6 0 1 69 1 0.0 6 1 70 1 0.0 6 1 71 1 0.0 6 1 72 4 0.0 6 4 73 10 0.0 6 10 74 8 0.0 6 8 75 26 0.0 6 26 76 12 0.0 6 12 77 7 0.0 6 7 78 15 0.0 6 15 79 46 0.0 6 46 80 36 0.0 6 36 81 44 0.0 6 43 1 82 29 0.0 6 28 0 0 0 1 83 9 0.0 6 3 0 0 0 6 84 5 0.0 6 4 0 0 0 1 85 46 0.0 6 42 0 0 0 4 86 47 0.0 6 41 0 0 0 6 87 21 0.0 6 21 88 7 0.0 6 6 0 0 1 89 19 0.0 6 13 0 0 0 6 90 30 0.0 6 26 0 0 0 4 91 43 0.0 6 36 0 0 0 7 92 31 0.0 6 30 0 0 0 1 93 24 0.0 6 22 1 0 1 94 26 0.0 6 22 0 0 0 4 95 12 0.0 6 9 0 0 1 2 96 11 0.0 6 10 0 0 0 1 97 39 0.0 6 38 0 0 0 1 98 38 0.0 6 34 0 0 0 4 99 8 0.0 6 5 0 0 1 2 100 1 0.0 6 0 0 0 0 1 101 3 0.0 6 3 102 12 0.0 6 11 0 0 0 1 103 12 0.0 6 11 0 0 0 1 104 15 0.0 6 15 105 27 0.0 6 27 106 16 0.0 6 16 107 5 0.0 6 4 0 0 0 1 108 12 0.0 6 9 0 0 0 3 109 10 0.0 6 7 0 0 0 3 110 17 0.0 6 16 0 0 0 1 111 6 0.0 6 6 112 9 0.0 6 9 113 14 0.0 6 13 0 0 0 1 114 2 0.0 6 1 0 0 0 1 115 1 0.0 6 1 116 9 0.0 6 9 117 9 0.0 6 7 0 0 0 2 118 11 0.0 6 10 0 0 0 1 119 1 0.0 6 1 120 4 0.0 6 3 0 0 0 1 121 13 0.0 6 11 0 0 0 2 122 117 0.0 6 117 123 13 0.0 6 12 1 124 26 0.0 6 26 125 30 0.0 6 25 0 0 0 5 126 39 0.0 6 28 0 0 0 11 127 12 0.0 6 12 128 6 0.0 6 5 0 0 0 1 129 14 0.0 6 7 0 0 0 7 130 16 0.0 6 11 0 0 0 5 131 54 0.0 6 53 0 0 0 1 132 8 0.0 6 8 133 8 0.0 6 8 134 6 0.0 6 5 0 0 0 1 135 18 0.0 6 16 0 0 0 2 136 24 0.0 6 24 137 51 0.0 6 51 138 19 0.0 6 15 0 1 0 3 139 18 0.0 6 12 0 0 0 6 140 41 0.0 6 39 0 0 0 2 141 5 0.0 6 2 0 0 0 3 142 5 0.0 6 5 143 12 0.0 6 12 144 14 0.0 6 11 0 0 0 3 145 46 0.0 6 45 0 0 0 1 146 2 0.0 6 2 147 1 0.0 6 0 0 0 0 1 148 7 0.0 6 7 149 17 0.0 6 16 0 0 0 1 150 23 0.0 6 23 151 43 0.0 6 43 152 6 0.0 6 6 153 18 0.0 6 18 154 1 0.0 6 1 155 7 0.0 6 5 0 0 0 2 156 19 0.0 6 17 1 0 0 1 157 40 0.0 6 38 1 0 0 1 158 18 0.0 6 18 159 4 0.0 6 4 160 6 0.0 6 5 0 0 0 1 161 9 0.0 6 8 0 0 0 1 162 19 0.0 6 16 0 0 0 3 163 8 0.0 6 8 164 3 0.0 6 3 165 8 0.0 6 7 1 166 28 0.0 6 27 0 0 0 1 167 25 0.0 6 25 168 85 0.0 6 85 169 57 0.0 6 56 0 0 0 1 170 52 0.0 6 45 0 0 1 6 172 11 0.0 6 8 0 0 0 3 173 25 0.0 6 20 0 0 0 5 174 13 0.0 6 12 0 0 0 1 175 3 0.0 6 3 176 3 0.0 6 3 177 3 0.0 6 1 1 0 0 1 178 3 0.0 6 3 179 13 0.0 6 12 1 180 13 0.0 6 13 181 17 0.0 6 17 182 39 0.0 6 38 1 183 10 0.0 6 8 0 0 0 2 184 11 0.0 6 11 185 4 0.0 6 3 0 0 1 186 5 0.0 6 2 0 0 0 3 188 7 0.0 6 7 191 2 0.0 6 2 192 5 0.0 6 5 193 2 0.0 6 1 1 194 3 0.0 6 3 195 11 0.0 6 11 196 2 0.0 6 1 0 0 0 1 197 2 0.0 6 2 198 1 0.0 6 1 199 2 0.0 6 1 0 0 0 1 200 3 0.0 6 3 203 1 0.0 6 1 204 4 0.0 6 4 206 1 0.0 6 1 207 1 0.0 6 1 211 8 0.0 6 8 212 33 0.0 6 33 213 2 0.0 6 2 214 1 0.0 6 1 216 1 0.0 6 0 0 0 0 1 217 2 0.0 6 2 218 1 0.0 6 1 222 1 0.0 6 1 225 1 0.0 6 1 226 2 0.0 6 2 228 1 0.0 6 1 229 1 0.0 6 1 230 1 0.0 6 1 237 2 0.0 6 2 245 1 0.0 6 1 249 1 0.0 6 1 256 1 0.0 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_11_224_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_11_451_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_11_224_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_11_451_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 5.83 s (58 µs/read; 1.04 M reads/minute). === Summary === Total read pairs processed: 101,346 Read 1 with adapter: 101,341 (100.0%) Read 2 with adapter: 101,323 (100.0%) == Read fate breakdown == Pairs that were too short: 4 (0.0%) Pairs discarded as untrimmed: 28 (0.0%) Pairs written (passing filters): 101,314 (100.0%) Total basepairs processed: 52,277,939 bp Read 1: 29,534,960 bp Read 2: 22,742,979 bp Total written (filtered): 6,308,880 bp (12.1%) Read 1: 3,364,885 bp Read 2: 2,943,995 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 100895 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 2 1583.5 0 1 1 4 2 395.9 1 1 1 6 5 24.7 1 1 0 4 7 1 6.2 2 1 9 1 0.4 2 1 15 2 0.0 4 2 16 2 0.0 4 2 18 1 0.0 5 1 19 1 0.0 5 1 20 19 0.0 6 17 2 21 8 0.0 6 7 1 22 12 0.0 6 11 1 23 10 0.0 6 10 24 6 0.0 6 6 25 12 0.0 6 12 26 7 0.0 6 7 27 5 0.0 6 5 28 3 0.0 6 3 29 1 0.0 6 1 31 6 0.0 6 6 33 4 0.0 6 3 1 34 3 0.0 6 2 1 35 1 0.0 6 0 1 36 6 0.0 6 6 37 4 0.0 6 4 38 8 0.0 6 6 2 40 7 0.0 6 7 41 2 0.0 6 0 2 42 2 0.0 6 2 43 1 0.0 6 1 44 10 0.0 6 9 1 45 12 0.0 6 12 46 12 0.0 6 11 1 47 8 0.0 6 6 0 2 48 2 0.0 6 0 1 0 1 49 3 0.0 6 3 50 5 0.0 6 5 51 3 0.0 6 3 52 2 0.0 6 2 53 3 0.0 6 3 54 5 0.0 6 4 0 0 0 1 55 3 0.0 6 3 56 9 0.0 6 8 1 57 3 0.0 6 2 1 59 2 0.0 6 2 60 3 0.0 6 3 61 1 0.0 6 1 62 9 0.0 6 9 63 5 0.0 6 5 64 5 0.0 6 4 1 65 8 0.0 6 8 66 2 0.0 6 2 67 2 0.0 6 2 69 2 0.0 6 1 1 70 2 0.0 6 1 1 71 2 0.0 6 2 73 1 0.0 6 1 74 2 0.0 6 2 75 2 0.0 6 2 77 4 0.0 6 4 78 6 0.0 6 3 2 1 79 3 0.0 6 1 2 81 3 0.0 6 3 84 1 0.0 6 1 85 2 0.0 6 1 1 86 12 0.0 6 12 87 1 0.0 6 1 88 2 0.0 6 1 1 89 7 0.0 6 7 90 5 0.0 6 4 1 91 5 0.0 6 5 92 6 0.0 6 6 93 2 0.0 6 1 1 94 3 0.0 6 3 95 1 0.0 6 1 97 3 0.0 6 3 98 9 0.0 6 7 2 99 4 0.0 6 4 100 7 0.0 6 7 101 16 0.0 6 12 3 0 1 102 14 0.0 6 13 1 103 6 0.0 6 6 104 8 0.0 6 6 1 0 0 0 0 1 105 7 0.0 6 6 1 106 5 0.0 6 4 1 107 14 0.0 6 13 1 108 8 0.0 6 8 109 7 0.0 6 6 1 110 3 0.0 6 3 111 7 0.0 6 7 112 16 0.0 6 14 1 1 113 14 0.0 6 13 1 114 7 0.0 6 7 115 16 0.0 6 13 2 1 116 9 0.0 6 8 1 117 9 0.0 6 8 1 118 8 0.0 6 8 119 8 0.0 6 8 120 5 0.0 6 4 1 121 9 0.0 6 8 1 122 26 0.0 6 25 1 123 21 0.0 6 19 2 124 16 0.0 6 13 3 125 13 0.0 6 12 1 126 16 0.0 6 14 1 0 1 127 11 0.0 6 11 128 44 0.0 6 39 4 0 0 0 0 1 129 14 0.0 6 14 130 11 0.0 6 11 131 11 0.0 6 9 1 0 1 132 19 0.0 6 18 1 133 16 0.0 6 16 134 27 0.0 6 26 1 135 16 0.0 6 16 136 20 0.0 6 20 137 42 0.0 6 37 4 1 138 34 0.0 6 32 2 139 29 0.0 6 28 1 140 42 0.0 6 39 3 141 85 0.0 6 85 142 62 0.0 6 61 1 143 43 0.0 6 43 144 50 0.0 6 48 2 145 107 0.0 6 104 3 146 162 0.0 6 160 1 1 147 66 0.0 6 65 1 148 47 0.0 6 46 1 149 54 0.0 6 54 150 22 0.0 6 21 0 1 151 32 0.0 6 29 3 152 50 0.0 6 48 2 153 99 0.0 6 97 2 154 34 0.0 6 29 5 155 17 0.0 6 16 1 156 42 0.0 6 39 2 0 0 0 1 157 34 0.0 6 32 2 158 49 0.0 6 46 3 159 83 0.0 6 81 2 160 52 0.0 6 48 4 161 92 0.0 6 92 162 60 0.0 6 60 163 48 0.0 6 47 1 164 108 0.0 6 104 3 1 165 38 0.0 6 38 166 31 0.0 6 29 1 1 167 41 0.0 6 41 168 85 0.0 6 79 6 169 73 0.0 6 68 5 170 78 0.0 6 75 2 1 171 70 0.0 6 67 3 172 98 0.0 6 97 1 173 58 0.0 6 56 1 0 0 0 1 174 101 0.0 6 97 4 175 58 0.0 6 55 3 176 46 0.0 6 44 1 1 177 70 0.0 6 70 178 193 0.0 6 191 2 179 227 0.0 6 225 2 180 136 0.0 6 134 2 181 37 0.0 6 35 1 0 0 0 0 1 182 49 0.0 6 43 6 183 85 0.0 6 80 5 184 78 0.0 6 75 3 185 125 0.0 6 123 2 186 51 0.0 6 46 4 1 187 98 0.0 6 94 4 188 175 0.0 6 173 2 189 69 0.0 6 65 4 190 80 0.0 6 76 4 191 95 0.0 6 88 7 192 164 0.0 6 150 13 1 193 162 0.0 6 154 8 194 188 0.0 6 183 4 1 195 227 0.0 6 211 15 1 196 173 0.0 6 161 11 1 197 139 0.0 6 133 5 1 198 120 0.0 6 117 3 199 82 0.0 6 77 4 0 0 0 1 200 93 0.0 6 91 2 201 92 0.0 6 89 2 0 0 1 202 106 0.0 6 102 4 203 195 0.0 6 189 5 1 204 145 0.0 6 140 4 1 205 236 0.0 6 224 10 0 1 1 206 184 0.0 6 170 12 2 207 196 0.0 6 187 9 208 277 0.0 6 264 12 1 209 516 0.0 6 487 29 210 351 0.0 6 311 39 0 0 1 211 729 0.0 6 673 55 1 212 457 0.0 6 433 23 0 0 1 213 329 0.0 6 313 15 1 214 701 0.0 6 655 45 1 215 191 0.0 6 175 15 1 216 721 0.0 6 681 39 1 217 233 0.0 6 224 9 218 262 0.0 6 247 14 1 219 195 0.0 6 190 4 1 220 278 0.0 6 261 17 221 146 0.0 6 134 12 222 382 0.0 6 356 26 223 219 0.0 6 212 7 224 445 0.0 6 406 39 225 292 0.0 6 270 22 226 927 0.0 6 868 58 1 227 560 0.0 6 525 31 4 228 389 0.0 6 362 27 229 682 0.0 6 627 52 2 0 1 230 330 0.0 6 309 21 231 360 0.0 6 346 13 1 232 309 0.0 6 293 16 233 245 0.0 6 239 6 234 258 0.0 6 246 12 235 270 0.0 6 255 14 1 236 242 0.0 6 230 11 1 237 328 0.0 6 311 16 1 238 200 0.0 6 193 7 239 357 0.0 6 334 22 1 240 955 0.0 6 899 52 3 1 241 201 0.0 6 189 11 1 242 292 0.0 6 273 19 243 187 0.0 6 176 11 244 140 0.0 6 135 4 1 245 141 0.0 6 129 12 246 228 0.0 6 213 14 0 1 247 644 0.0 6 600 44 248 329 0.0 6 304 23 2 249 305 0.0 6 288 17 250 695 0.0 6 640 51 4 251 378 0.0 6 344 34 252 1896 0.0 6 1780 114 2 253 434 0.0 6 396 38 254 394 0.0 6 356 35 3 255 522 0.0 6 480 40 2 256 1006 0.0 6 931 72 1 2 257 1429 0.0 6 1345 80 4 258 1149 0.0 6 1077 68 3 0 1 259 1862 0.0 6 1759 99 3 1 260 559 0.0 6 517 41 0 0 1 261 1187 0.0 6 1092 92 3 262 554 0.0 6 512 41 0 0 1 263 900 0.0 6 839 60 0 1 264 587 0.0 6 552 34 1 265 307 0.0 6 273 32 2 266 798 0.0 6 758 39 1 267 1032 0.0 6 988 44 268 487 0.0 6 454 30 3 269 971 0.0 6 899 69 3 270 637 0.0 6 588 47 2 271 858 0.0 6 798 59 1 272 627 0.0 6 588 35 4 273 871 0.0 6 820 50 1 274 700 0.0 6 640 59 1 275 819 0.0 6 744 67 6 1 0 0 1 276 4656 0.0 6 4376 269 8 3 277 4229 0.0 6 3969 250 7 2 0 1 278 46372 0.0 6 43618 2709 42 1 1 1 279 1034 0.0 6 933 94 7 280 7 0.0 6 6 1 281 6 0.0 6 5 1 284 1 0.0 6 1 285 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 446 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 73 3 0.0 6 3 74 7 0.0 6 7 75 8 0.0 6 8 76 3 0.0 6 3 78 7 0.0 6 7 79 11 0.0 6 11 80 3 0.0 6 3 82 4 0.0 6 4 83 2 0.0 6 2 85 1 0.0 6 1 86 6 0.0 6 6 89 1 0.0 6 1 90 3 0.0 6 3 91 5 0.0 6 5 92 7 0.0 6 7 93 2 0.0 6 2 94 11 0.0 6 11 96 3 0.0 6 3 97 10 0.0 6 10 99 1 0.0 6 1 100 1 0.0 6 1 101 1 0.0 6 1 102 5 0.0 6 5 103 1 0.0 6 1 104 1 0.0 6 1 105 6 0.0 6 6 106 3 0.0 6 3 107 3 0.0 6 3 108 1 0.0 6 1 109 1 0.0 6 1 110 8 0.0 6 8 111 11 0.0 6 11 112 21 0.0 6 21 113 4 0.0 6 4 114 1 0.0 6 1 116 1 0.0 6 1 117 4 0.0 6 4 118 3 0.0 6 3 120 3 0.0 6 3 121 5 0.0 6 5 125 1 0.0 6 1 129 3 0.0 6 3 130 1 0.0 6 1 131 5 0.0 6 5 135 1 0.0 6 1 136 9 0.0 6 9 139 12 0.0 6 12 140 3 0.0 6 3 141 16 0.0 6 16 144 4 0.0 6 4 145 5 0.0 6 5 146 4 0.0 6 4 147 3 0.0 6 3 148 1 0.0 6 1 149 1 0.0 6 1 151 3 0.0 6 3 152 6 0.0 6 6 153 2 0.0 6 2 154 4 0.0 6 4 155 4 0.0 6 4 156 25 0.0 6 25 157 1 0.0 6 1 158 3 0.0 6 3 162 2 0.0 6 2 166 2 0.0 6 2 167 4 0.0 6 3 1 168 14 0.0 6 14 169 17 0.0 6 17 170 2 0.0 6 2 171 11 0.0 6 11 172 1 0.0 6 1 173 2 0.0 6 2 174 2 0.0 6 2 175 2 0.0 6 2 176 5 0.0 6 5 182 1 0.0 6 1 183 6 0.0 6 6 184 4 0.0 6 4 185 6 0.0 6 6 188 2 0.0 6 2 189 2 0.0 6 2 190 2 0.0 6 2 192 1 0.0 6 1 194 4 0.0 6 4 195 10 0.0 6 10 199 3 0.0 6 3 200 3 0.0 6 3 201 2 0.0 6 2 203 1 0.0 6 1 204 4 0.0 6 4 205 1 0.0 6 1 208 3 0.0 6 3 211 1 0.0 6 1 212 2 0.0 6 2 220 3 0.0 6 3 223 1 0.0 6 1 225 1 0.0 6 1 226 2 0.0 6 2 227 11 0.0 6 11 229 1 0.0 6 1 243 2 0.0 6 2 248 1 0.0 6 1 255 1 0.0 6 1 262 2 0.0 6 2 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 101133 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 4 1 395.9 1 0 1 6 1 24.7 1 1 7 2 6.2 2 2 11 1 0.0 3 1 12 5 0.0 3 5 13 18 0.0 3 13 3 0 2 14 6 0.0 4 6 15 2 0.0 4 1 0 1 16 2 0.0 4 2 17 1 0.0 5 1 18 3 0.0 5 1 2 19 15 0.0 5 15 20 53 0.0 6 53 21 54 0.0 6 52 2 22 40 0.0 6 34 6 23 24 0.0 6 24 24 23 0.0 6 23 25 13 0.0 6 11 2 26 15 0.0 6 15 27 11 0.0 6 10 1 28 18 0.0 6 18 29 18 0.0 6 16 2 30 17 0.0 6 14 3 31 7 0.0 6 5 2 32 2 0.0 6 2 33 13 0.0 6 11 2 34 12 0.0 6 11 1 35 11 0.0 6 10 0 1 36 2 0.0 6 2 37 7 0.0 6 5 2 38 19 0.0 6 16 3 39 10 0.0 6 9 1 40 7 0.0 6 7 41 4 0.0 6 4 42 8 0.0 6 8 43 7 0.0 6 7 44 17 0.0 6 15 2 45 8 0.0 6 5 2 0 0 1 46 20 0.0 6 18 2 47 10 0.0 6 9 1 48 17 0.0 6 16 1 49 21 0.0 6 18 2 0 1 50 18 0.0 6 14 4 51 19 0.0 6 18 1 52 25 0.0 6 20 5 53 14 0.0 6 13 1 54 8 0.0 6 5 3 55 26 0.0 6 25 1 56 37 0.0 6 33 4 57 19 0.0 6 15 4 58 17 0.0 6 16 1 59 13 0.0 6 12 0 0 1 60 15 0.0 6 13 2 61 11 0.0 6 9 2 62 11 0.0 6 10 1 63 13 0.0 6 12 1 64 10 0.0 6 9 1 65 8 0.0 6 7 1 66 18 0.0 6 16 2 67 31 0.0 6 28 2 1 68 15 0.0 6 11 3 1 69 44 0.0 6 42 1 1 70 192 0.0 6 184 7 1 71 27 0.0 6 24 2 0 1 72 99 0.0 6 97 1 0 0 1 73 18 0.0 6 18 74 41 0.0 6 38 2 1 75 19 0.0 6 18 1 76 12 0.0 6 11 1 77 19 0.0 6 16 2 1 78 27 0.0 6 27 79 13 0.0 6 13 80 22 0.0 6 19 2 0 0 1 81 11 0.0 6 10 0 0 0 1 82 29 0.0 6 23 5 1 83 40 0.0 6 35 4 1 84 49 0.0 6 45 3 0 0 1 85 54 0.0 6 49 3 1 1 86 80 0.0 6 74 6 87 58 0.0 6 54 3 1 88 25 0.0 6 25 89 17 0.0 6 16 1 90 41 0.0 6 36 5 91 32 0.0 6 30 2 92 25 0.0 6 23 2 93 38 0.0 6 31 7 94 95 0.0 6 88 6 1 95 30 0.0 6 27 2 1 96 55 0.0 6 49 5 0 0 1 97 41 0.0 6 41 98 31 0.0 6 31 99 67 0.0 6 59 6 1 0 0 1 100 52 0.0 6 49 3 101 52 0.0 6 47 5 102 81 0.0 6 74 4 2 0 0 1 103 89 0.0 6 85 4 104 82 0.0 6 69 10 1 0 1 1 105 76 0.0 6 76 106 46 0.0 6 41 4 0 0 1 107 85 0.0 6 80 5 108 36 0.0 6 35 1 109 41 0.0 6 40 1 110 59 0.0 6 57 2 111 46 0.0 6 42 3 0 0 1 112 79 0.0 6 72 7 113 53 0.0 6 50 3 114 47 0.0 6 43 4 115 71 0.0 6 67 3 0 0 1 116 43 0.0 6 42 1 117 38 0.0 6 37 1 118 69 0.0 6 62 6 1 119 68 0.0 6 59 7 1 0 1 120 92 0.0 6 84 8 121 49 0.0 6 45 4 122 57 0.0 6 51 5 0 0 1 123 57 0.0 6 53 4 124 95 0.0 6 92 3 125 59 0.0 6 58 1 126 35 0.0 6 32 3 127 73 0.0 6 69 3 0 0 0 1 128 72 0.0 6 70 2 129 79 0.0 6 68 10 1 130 70 0.0 6 61 9 131 114 0.0 6 103 10 1 132 134 0.0 6 128 6 133 79 0.0 6 72 6 0 0 1 134 116 0.0 6 101 14 1 135 269 0.0 6 254 14 1 136 166 0.0 6 157 8 1 137 233 0.0 6 223 10 138 129 0.0 6 122 7 139 182 0.0 6 173 7 2 140 234 0.0 6 225 9 141 261 0.0 6 253 8 142 413 0.0 6 395 17 1 143 302 0.0 6 295 7 144 439 0.0 6 419 17 1 1 0 1 145 301 0.0 6 289 11 0 0 1 146 317 0.0 6 309 8 147 274 0.0 6 268 6 148 243 0.0 6 232 11 149 374 0.0 6 362 12 150 475 0.0 6 456 16 2 0 0 1 151 442 0.0 6 425 16 0 0 1 152 618 0.0 6 593 24 1 153 568 0.0 6 543 22 2 0 0 1 154 386 0.0 6 373 12 0 0 1 155 403 0.0 6 386 17 156 405 0.0 6 395 6 1 1 1 1 157 416 0.0 6 403 13 158 495 0.0 6 478 15 1 0 1 159 246 0.0 6 234 10 1 0 1 160 561 0.0 6 544 16 1 161 376 0.0 6 364 10 2 162 315 0.0 6 301 13 1 163 468 0.0 6 451 16 0 0 1 164 440 0.0 6 426 12 1 1 165 554 0.0 6 535 19 166 893 0.0 6 861 31 1 167 496 0.0 6 480 16 168 835 0.0 6 814 20 1 169 506 0.0 6 489 13 3 0 1 170 538 0.0 6 516 21 1 171 679 0.0 6 654 23 2 172 348 0.0 6 336 10 2 173 557 0.0 6 534 23 174 573 0.0 6 548 25 175 938 0.0 6 900 37 0 0 1 176 682 0.0 6 652 25 3 1 1 177 1191 0.0 6 1152 37 1 0 1 178 663 0.0 6 638 24 1 179 787 0.0 6 753 32 0 1 1 180 948 0.0 6 916 30 2 181 1108 0.0 6 1067 36 4 1 182 489 0.0 6 474 14 0 1 183 831 0.0 6 796 32 2 0 1 184 358 0.0 6 348 9 1 185 365 0.0 6 348 14 1 2 186 1139 0.0 6 1095 43 0 0 1 187 1063 0.0 6 1012 49 0 0 1 1 188 1701 0.0 6 1649 45 6 0 1 189 1590 0.0 6 1531 55 1 1 2 190 1955 0.0 6 1889 65 0 1 191 1924 0.0 6 1856 63 1 0 4 192 1137 0.0 6 1088 45 4 193 1578 0.0 6 1544 31 1 1 1 194 1499 0.0 6 1450 44 3 0 2 195 1196 0.0 6 1143 53 196 2672 0.0 6 2579 89 3 0 1 197 649 0.0 6 619 28 1 1 198 2375 0.0 6 2303 67 2 1 2 199 720 0.0 6 685 33 1 1 200 1997 0.0 6 1934 62 1 201 1954 0.0 6 1880 70 2 0 1 0 1 202 1662 0.0 6 1604 55 3 203 1185 0.0 6 1154 28 1 0 1 1 204 1310 0.0 6 1265 41 2 0 2 205 1584 0.0 6 1519 59 1 4 1 206 1127 0.0 6 1088 36 3 207 1071 0.0 6 1021 49 0 0 1 208 2617 0.0 6 2524 90 1 0 1 1 209 372 0.0 6 357 13 2 210 1072 0.0 6 1033 34 3 0 2 211 1736 0.0 6 1686 48 0 0 1 1 212 1117 0.0 6 1079 35 0 0 2 1 213 229 0.0 6 219 9 1 214 1295 0.0 6 1246 46 2 0 1 215 1427 0.0 6 1364 60 2 0 1 216 1391 0.0 6 1340 44 4 0 3 217 701 0.0 6 685 14 0 2 218 574 0.0 6 561 12 1 219 556 0.0 6 540 16 220 365 0.0 6 350 13 1 0 1 221 495 0.0 6 477 17 1 222 496 0.0 6 478 17 0 0 1 223 241 0.0 6 235 6 224 398 0.0 6 386 11 1 225 539 0.0 6 509 30 226 1125 0.0 6 1080 42 3 227 2180 0.0 6 2112 67 1 228 1293 0.0 6 1253 32 4 2 2 229 1381 0.0 6 1309 72 230 2728 0.0 6 2630 94 3 0 1 231 1239 0.0 6 1193 46 232 3200 0.0 6 3110 82 2 0 5 1 233 389 0.0 6 370 16 2 0 1 234 924 0.0 6 894 29 1 235 719 0.0 6 698 20 0 0 0 1 236 495 0.0 6 477 16 0 0 2 237 743 0.0 6 718 24 1 238 362 0.0 6 351 11 239 68 0.0 6 66 2 240 146 0.0 6 142 4 241 386 0.0 6 374 11 0 1 242 212 0.0 6 204 8 243 53 0.0 6 50 3 244 164 0.0 6 152 12 245 800 0.0 6 768 31 1 246 354 0.0 6 342 12 247 362 0.0 6 354 8 248 192 0.0 6 184 6 1 1 249 386 0.0 6 374 11 0 0 1 250 232 0.0 6 222 10 251 680 0.0 6 651 27 1 0 1 252 293 0.0 6 282 10 0 1 253 90 0.0 6 85 5 254 776 0.0 6 751 24 0 0 1 255 125 0.0 6 123 2 256 98 0.0 6 97 1 257 12 0.0 6 12 258 6 0.0 6 6 259 8 0.0 6 8 260 2 0.0 6 2 262 2 0.0 6 2 264 1 0.0 6 1 265 4 0.0 6 4 267 1 0.0 6 1 269 2 0.0 6 2 270 1 0.0 6 1 275 1 0.0 6 1 279 1 0.0 6 1 280 22 0.0 6 21 1 281 60 0.0 6 59 1 282 397 0.0 6 384 12 1 284 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 190 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 65 1 0.0 6 1 69 1 0.0 6 1 70 3 0.0 6 3 71 3 0.0 6 3 72 2 0.0 6 2 73 1 0.0 6 1 74 4 0.0 6 4 75 6 0.0 6 6 78 3 0.0 6 3 80 1 0.0 6 0 0 0 0 1 82 2 0.0 6 2 86 2 0.0 6 2 87 2 0.0 6 2 88 1 0.0 6 1 89 3 0.0 6 3 90 1 0.0 6 1 92 1 0.0 6 1 93 2 0.0 6 2 97 2 0.0 6 2 100 1 0.0 6 1 101 6 0.0 6 6 103 1 0.0 6 1 106 1 0.0 6 1 107 2 0.0 6 2 108 7 0.0 6 7 109 2 0.0 6 2 111 1 0.0 6 1 112 2 0.0 6 1 0 0 0 1 113 1 0.0 6 1 114 5 0.0 6 5 115 1 0.0 6 1 116 1 0.0 6 1 117 1 0.0 6 1 118 2 0.0 6 2 125 1 0.0 6 1 126 1 0.0 6 1 130 1 0.0 6 1 132 1 0.0 6 1 134 2 0.0 6 2 135 9 0.0 6 9 136 2 0.0 6 2 137 5 0.0 6 5 139 1 0.0 6 0 0 0 0 1 141 4 0.0 6 4 142 2 0.0 6 2 145 1 0.0 6 1 148 4 0.0 6 4 150 1 0.0 6 1 151 6 0.0 6 6 152 11 0.0 6 10 0 0 0 1 153 1 0.0 6 1 155 1 0.0 6 1 156 1 0.0 6 0 0 0 0 1 158 4 0.0 6 4 162 1 0.0 6 1 163 5 0.0 6 5 164 10 0.0 6 9 0 0 1 165 6 0.0 6 6 166 2 0.0 6 2 167 5 0.0 6 5 170 3 0.0 6 3 171 1 0.0 6 1 178 3 0.0 6 3 179 5 0.0 6 5 181 4 0.0 6 4 183 1 0.0 6 1 184 1 0.0 6 1 185 2 0.0 6 2 190 1 0.0 6 1 191 2 0.0 6 2 201 1 0.0 6 1 216 1 0.0 6 1 225 2 0.0 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_12_225_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_12_452_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_12_225_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_12_452_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 2.55 s (104 µs/read; 0.58 M reads/minute). === Summary === Total read pairs processed: 24,475 Read 1 with adapter: 24,474 (100.0%) Read 2 with adapter: 24,454 (99.9%) == Read fate breakdown == Pairs that were too short: 14 (0.1%) Pairs discarded as untrimmed: 22 (0.1%) Pairs written (passing filters): 24,439 (99.9%) Total basepairs processed: 10,521,148 bp Read 1: 5,830,332 bp Read 2: 4,690,816 bp Total written (filtered): 1,628,811 bp (15.5%) Read 1: 852,123 bp Read 2: 776,688 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 23504 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 3 1 382.4 0 1 6 3 6.0 1 0 0 3 7 1 1.5 2 1 8 1 0.4 2 1 9 2 0.1 2 1 0 1 10 1 0.0 3 1 14 2 0.0 4 2 18 1 0.0 5 1 19 1 0.0 5 1 20 28 0.0 6 27 1 21 11 0.0 6 11 22 13 0.0 6 13 23 7 0.0 6 6 1 24 3 0.0 6 3 25 3 0.0 6 3 26 5 0.0 6 4 1 27 3 0.0 6 2 1 28 2 0.0 6 2 29 1 0.0 6 1 30 2 0.0 6 2 31 1 0.0 6 1 32 1 0.0 6 1 33 2 0.0 6 1 1 34 5 0.0 6 4 0 1 35 1 0.0 6 1 36 2 0.0 6 2 37 2 0.0 6 2 38 2 0.0 6 2 39 2 0.0 6 1 0 0 0 1 40 4 0.0 6 4 41 4 0.0 6 4 43 1 0.0 6 1 44 1 0.0 6 1 45 3 0.0 6 2 1 46 2 0.0 6 2 47 2 0.0 6 2 48 1 0.0 6 1 49 3 0.0 6 3 50 1 0.0 6 1 51 1 0.0 6 1 52 2 0.0 6 2 55 4 0.0 6 2 2 57 1 0.0 6 1 58 1 0.0 6 1 59 1 0.0 6 1 62 1 0.0 6 1 63 1 0.0 6 1 64 3 0.0 6 3 65 1 0.0 6 1 66 4 0.0 6 4 67 1 0.0 6 1 68 1 0.0 6 1 69 3 0.0 6 3 70 2 0.0 6 2 71 3 0.0 6 3 72 4 0.0 6 4 73 4 0.0 6 4 74 4 0.0 6 4 75 2 0.0 6 2 76 2 0.0 6 2 78 1 0.0 6 1 79 4 0.0 6 4 80 5 0.0 6 5 81 2 0.0 6 2 82 4 0.0 6 4 83 9 0.0 6 7 2 84 2 0.0 6 2 85 3 0.0 6 3 86 3 0.0 6 3 87 3 0.0 6 3 88 2 0.0 6 2 89 3 0.0 6 3 90 2 0.0 6 2 91 1 0.0 6 1 92 3 0.0 6 2 1 93 5 0.0 6 5 94 2 0.0 6 2 95 3 0.0 6 3 96 1 0.0 6 1 97 3 0.0 6 3 98 2 0.0 6 2 99 4 0.0 6 4 100 1 0.0 6 1 101 9 0.0 6 9 102 5 0.0 6 5 103 6 0.0 6 6 104 4 0.0 6 4 105 2 0.0 6 2 106 5 0.0 6 5 108 3 0.0 6 3 109 10 0.0 6 10 110 5 0.0 6 5 111 8 0.0 6 7 1 112 12 0.0 6 12 113 9 0.0 6 9 114 6 0.0 6 6 115 8 0.0 6 8 116 11 0.0 6 9 1 1 117 6 0.0 6 6 118 4 0.0 6 4 119 6 0.0 6 5 1 120 8 0.0 6 8 121 8 0.0 6 7 1 122 6 0.0 6 6 123 8 0.0 6 8 124 9 0.0 6 9 125 12 0.0 6 11 1 126 4 0.0 6 4 127 8 0.0 6 8 128 8 0.0 6 7 1 129 11 0.0 6 10 1 130 5 0.0 6 5 131 5 0.0 6 5 132 9 0.0 6 9 133 12 0.0 6 12 134 10 0.0 6 10 135 11 0.0 6 11 136 12 0.0 6 12 137 17 0.0 6 17 138 15 0.0 6 15 139 22 0.0 6 21 1 140 19 0.0 6 19 141 38 0.0 6 38 142 220 0.0 6 220 143 146 0.0 6 146 144 81 0.0 6 81 145 341 0.0 6 341 146 175 0.0 6 174 1 147 93 0.0 6 91 2 148 94 0.0 6 94 149 238 0.0 6 234 4 150 72 0.0 6 72 151 100 0.0 6 99 1 152 74 0.0 6 73 1 153 190 0.0 6 189 0 0 0 1 154 45 0.0 6 44 1 155 182 0.0 6 182 156 72 0.0 6 71 1 157 50 0.0 6 49 1 158 24 0.0 6 23 1 159 31 0.0 6 31 160 91 0.0 6 91 161 85 0.0 6 82 3 162 120 0.0 6 118 2 163 90 0.0 6 89 0 0 0 0 1 164 55 0.0 6 54 1 165 82 0.0 6 82 166 119 0.0 6 118 1 167 71 0.0 6 70 1 168 70 0.0 6 68 2 169 67 0.0 6 67 170 86 0.0 6 85 1 171 220 0.0 6 219 1 172 618 0.0 6 616 2 173 121 0.0 6 121 174 72 0.0 6 72 175 127 0.0 6 126 1 176 359 0.0 6 358 1 177 612 0.0 6 610 1 0 0 1 178 98 0.0 6 98 179 67 0.0 6 65 2 180 66 0.0 6 64 2 181 97 0.0 6 95 2 182 129 0.0 6 128 1 183 74 0.0 6 72 0 0 2 184 78 0.0 6 76 2 185 71 0.0 6 71 186 79 0.0 6 77 2 187 72 0.0 6 72 188 53 0.0 6 52 1 189 101 0.0 6 100 1 190 124 0.0 6 122 1 0 0 0 0 1 191 99 0.0 6 97 1 1 192 91 0.0 6 91 193 107 0.0 6 106 0 0 1 194 209 0.0 6 208 1 195 166 0.0 6 158 8 196 208 0.0 6 203 5 197 261 0.0 6 257 4 198 365 0.0 6 361 4 199 188 0.0 6 185 3 200 268 0.0 6 264 4 201 242 0.0 6 240 2 202 371 0.0 6 366 5 203 721 0.0 6 718 2 1 204 910 0.0 6 906 3 0 0 0 1 205 255 0.0 6 252 2 1 206 220 0.0 6 216 4 207 222 0.0 6 216 3 1 0 1 1 208 166 0.0 6 163 3 209 228 0.0 6 225 3 210 251 0.0 6 247 3 0 0 1 211 273 0.0 6 269 4 212 239 0.0 6 234 5 213 358 0.0 6 354 4 214 402 0.0 6 396 6 215 207 0.0 6 203 2 1 1 216 208 0.0 6 205 2 1 217 261 0.0 6 259 1 0 0 0 1 218 369 0.0 6 369 219 518 0.0 6 514 2 2 220 153 0.0 6 148 3 1 1 221 168 0.0 6 168 222 178 0.0 6 173 5 223 243 0.0 6 239 4 224 199 0.0 6 192 7 225 200 0.0 6 196 4 226 228 0.0 6 225 3 227 171 0.0 6 171 228 236 0.0 6 233 1 0 0 0 0 2 229 242 0.0 6 240 2 230 327 0.0 6 325 2 231 496 0.0 6 494 2 232 247 0.0 6 244 2 0 0 0 0 1 233 145 0.0 6 143 2 234 197 0.0 6 195 2 235 149 0.0 6 147 2 236 151 0.0 6 151 237 147 0.0 6 144 3 238 133 0.0 6 129 4 239 177 0.0 6 177 240 146 0.0 6 144 2 241 130 0.0 6 128 2 242 122 0.0 6 121 1 243 183 0.0 6 182 0 1 244 99 0.0 6 97 1 0 1 245 90 0.0 6 88 2 246 91 0.0 6 90 1 247 103 0.0 6 100 3 248 93 0.0 6 91 2 249 121 0.0 6 119 2 250 106 0.0 6 105 0 0 1 251 86 0.0 6 86 252 101 0.0 6 100 1 253 59 0.0 6 58 1 254 59 0.0 6 59 255 58 0.0 6 56 2 256 59 0.0 6 59 257 86 0.0 6 86 258 101 0.0 6 101 259 66 0.0 6 66 260 87 0.0 6 84 3 261 64 0.0 6 64 262 72 0.0 6 71 1 263 67 0.0 6 67 264 64 0.0 6 62 2 265 71 0.0 6 71 266 96 0.0 6 96 267 113 0.0 6 113 268 75 0.0 6 74 1 269 66 0.0 6 65 1 270 46 0.0 6 44 1 1 271 35 0.0 6 34 1 272 38 0.0 6 37 1 273 65 0.0 6 64 1 274 72 0.0 6 72 275 83 0.0 6 80 3 276 87 0.0 6 86 1 277 288 0.0 6 283 4 0 0 0 1 278 453 0.0 6 451 2 279 9 0.0 6 8 1 280 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 970 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 72 1 0.0 6 1 73 12 0.0 6 12 74 6 0.0 6 6 75 5 0.0 6 5 76 24 0.0 6 24 77 8 0.0 6 8 78 3 0.0 6 3 79 3 0.0 6 3 80 17 0.0 6 17 81 4 0.0 6 4 82 5 0.0 6 5 83 9 0.0 6 9 84 10 0.0 6 10 85 4 0.0 6 4 86 7 0.0 6 7 87 6 0.0 6 6 88 1 0.0 6 1 90 3 0.0 6 3 91 4 0.0 6 4 92 1 0.0 6 1 93 5 0.0 6 5 94 3 0.0 6 3 96 2 0.0 6 2 97 3 0.0 6 3 98 1 0.0 6 1 101 3 0.0 6 3 102 11 0.0 6 11 103 21 0.0 6 21 104 3 0.0 6 3 106 5 0.0 6 5 107 35 0.0 6 35 108 44 0.0 6 44 109 1 0.0 6 1 112 3 0.0 6 3 113 2 0.0 6 2 118 2 0.0 6 2 119 1 0.0 6 1 120 4 0.0 6 4 125 3 0.0 6 3 127 1 0.0 6 1 128 2 0.0 6 2 129 5 0.0 6 5 131 1 0.0 6 1 132 2 0.0 6 2 133 18 0.0 6 18 134 42 0.0 6 42 135 50 0.0 6 50 136 3 0.0 6 3 138 2 0.0 6 2 139 1 0.0 6 1 140 2 0.0 6 2 142 1 0.0 6 1 144 6 0.0 6 6 145 18 0.0 6 18 148 1 0.0 6 1 149 8 0.0 6 8 150 22 0.0 6 22 153 2 0.0 6 2 154 5 0.0 6 5 159 2 0.0 6 2 161 9 0.0 6 9 162 61 0.0 6 61 163 12 0.0 6 12 164 5 0.0 6 5 165 19 0.0 6 19 167 1 0.0 6 1 169 8 0.0 6 8 170 15 0.0 6 15 171 1 0.0 6 1 173 5 0.0 6 5 174 27 0.0 6 27 175 6 0.0 6 6 176 3 0.0 6 3 177 1 0.0 6 1 178 5 0.0 6 5 179 1 0.0 6 1 181 1 0.0 6 1 182 19 0.0 6 19 183 66 0.0 6 66 184 3 0.0 6 2 1 185 4 0.0 6 3 1 187 7 0.0 6 7 188 2 0.0 6 2 189 1 0.0 6 1 195 2 0.0 6 2 196 2 0.0 6 2 197 4 0.0 6 4 198 8 0.0 6 8 200 10 0.0 6 10 202 1 0.0 6 1 206 2 0.0 6 2 207 2 0.0 6 2 212 8 0.0 6 8 213 21 0.0 6 21 215 1 0.0 6 1 216 2 0.0 6 2 221 3 0.0 6 3 222 5 0.0 6 5 223 3 0.0 6 3 230 6 0.0 6 6 231 24 0.0 6 24 232 17 0.0 6 17 233 29 0.0 6 29 234 1 0.0 6 1 235 2 0.0 6 2 238 1 0.0 6 1 239 14 0.0 6 14 240 18 0.0 6 18 241 2 0.0 6 2 249 1 0.0 6 1 253 1 0.0 6 1 259 3 0.0 6 3 260 1 0.0 6 1 272 1 0.0 6 1 273 7 0.0 6 7 274 1 0.0 6 1 285 1 0.0 6 1 286 1 0.0 6 1 296 1 0.0 6 1 297 3 0.0 6 3 === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 23868 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 99.9% G: 0.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by 'C' extremely often. The provided adapter sequence could be incomplete at its 5' end. Ignore this warning when trimming primers. Overview of removed sequences length count expect max.err error counts 10 2 0.0 3 2 11 2 0.0 3 2 12 4 0.0 3 2 2 13 2 0.0 3 2 14 5 0.0 4 5 16 3 0.0 4 2 1 17 17 0.0 5 16 1 18 9 0.0 5 9 19 6 0.0 5 4 1 0 1 20 19 0.0 6 19 21 14 0.0 6 13 1 22 9 0.0 6 9 23 7 0.0 6 7 24 7 0.0 6 6 1 25 4 0.0 6 4 26 6 0.0 6 6 27 3 0.0 6 2 1 28 8 0.0 6 7 1 29 4 0.0 6 4 30 5 0.0 6 5 31 6 0.0 6 6 32 4 0.0 6 4 33 4 0.0 6 4 34 2 0.0 6 2 35 3 0.0 6 2 0 1 36 8 0.0 6 7 1 37 5 0.0 6 5 38 3 0.0 6 2 1 39 4 0.0 6 4 40 4 0.0 6 4 41 3 0.0 6 2 1 42 8 0.0 6 8 43 7 0.0 6 6 1 44 7 0.0 6 6 1 45 3 0.0 6 3 46 3 0.0 6 3 47 6 0.0 6 5 1 48 6 0.0 6 6 49 3 0.0 6 3 50 8 0.0 6 8 51 8 0.0 6 8 52 6 0.0 6 6 53 5 0.0 6 5 54 5 0.0 6 5 55 10 0.0 6 6 3 0 0 0 1 56 10 0.0 6 9 1 57 8 0.0 6 7 0 1 58 8 0.0 6 8 59 7 0.0 6 3 4 60 5 0.0 6 5 61 12 0.0 6 11 1 62 7 0.0 6 7 63 7 0.0 6 7 64 6 0.0 6 5 1 65 11 0.0 6 11 66 12 0.0 6 12 67 12 0.0 6 11 0 0 0 0 1 68 46 0.0 6 46 69 12 0.0 6 12 70 22 0.0 6 21 1 71 15 0.0 6 11 2 0 2 72 25 0.0 6 24 1 73 12 0.0 6 10 2 74 16 0.0 6 15 1 75 21 0.0 6 19 2 76 12 0.0 6 12 77 16 0.0 6 16 78 10 0.0 6 10 79 15 0.0 6 15 80 12 0.0 6 11 0 1 81 11 0.0 6 10 0 0 0 0 1 82 15 0.0 6 14 1 83 12 0.0 6 11 1 84 21 0.0 6 20 1 85 16 0.0 6 15 0 1 86 18 0.0 6 18 87 12 0.0 6 12 88 18 0.0 6 17 1 89 13 0.0 6 13 90 20 0.0 6 19 1 91 33 0.0 6 31 1 0 0 0 0 1 92 24 0.0 6 23 1 93 16 0.0 6 14 1 0 0 0 0 1 94 21 0.0 6 19 2 95 15 0.0 6 14 1 96 24 0.0 6 24 97 30 0.0 6 25 4 0 0 0 1 98 24 0.0 6 22 2 99 19 0.0 6 18 1 100 21 0.0 6 19 2 101 30 0.0 6 29 1 102 30 0.0 6 30 103 35 0.0 6 34 0 0 0 1 104 49 0.0 6 46 3 105 59 0.0 6 58 1 106 33 0.0 6 32 1 107 30 0.0 6 29 1 108 30 0.0 6 29 1 109 21 0.0 6 21 110 34 0.0 6 31 3 111 42 0.0 6 41 0 0 0 1 112 48 0.0 6 47 1 113 37 0.0 6 37 114 38 0.0 6 36 2 115 50 0.0 6 46 4 116 54 0.0 6 53 1 117 64 0.0 6 64 118 49 0.0 6 49 119 62 0.0 6 59 3 120 59 0.0 6 56 3 121 57 0.0 6 55 2 122 51 0.0 6 51 123 42 0.0 6 40 1 0 1 124 59 0.0 6 59 125 67 0.0 6 65 1 0 0 0 0 1 126 81 0.0 6 73 7 1 127 85 0.0 6 81 4 128 79 0.0 6 75 3 1 129 103 0.0 6 99 3 0 0 1 130 92 0.0 6 90 2 131 163 0.0 6 158 4 0 0 1 132 112 0.0 6 111 1 133 103 0.0 6 102 0 0 0 1 134 120 0.0 6 117 2 1 135 148 0.0 6 142 6 136 180 0.0 6 176 4 137 136 0.0 6 136 138 113 0.0 6 111 2 139 143 0.0 6 141 2 140 182 0.0 6 175 6 0 0 0 0 1 141 303 0.0 6 302 1 142 298 0.0 6 297 0 0 0 1 143 298 0.0 6 294 4 144 449 0.0 6 445 4 145 345 0.0 6 339 5 0 1 146 319 0.0 6 317 1 0 0 1 147 258 0.0 6 249 8 0 0 0 1 148 408 0.0 6 406 2 149 359 0.0 6 357 2 150 423 0.0 6 414 9 151 502 0.0 6 492 8 0 2 152 501 0.0 6 490 9 0 0 1 0 1 153 448 0.0 6 436 10 1 0 1 154 463 0.0 6 458 4 0 0 0 1 155 282 0.0 6 276 6 156 320 0.0 6 317 2 0 0 0 1 157 287 0.0 6 276 8 0 1 0 0 2 158 399 0.0 6 393 4 1 0 1 159 383 0.0 6 382 1 160 303 0.0 6 296 5 0 0 1 0 1 161 344 0.0 6 340 4 162 290 0.0 6 283 5 0 0 1 0 1 163 262 0.0 6 255 5 0 0 0 2 164 293 0.0 6 291 2 165 313 0.0 6 307 6 166 233 0.0 6 229 3 0 0 0 0 1 167 348 0.0 6 343 4 0 1 168 267 0.0 6 263 3 0 0 0 1 169 456 0.0 6 454 2 170 429 0.0 6 429 171 507 0.0 6 505 1 0 0 0 1 172 369 0.0 6 366 3 173 424 0.0 6 423 1 174 365 0.0 6 362 3 175 479 0.0 6 476 3 176 467 0.0 6 464 3 177 334 0.0 6 333 1 178 233 0.0 6 230 2 0 0 0 0 1 179 352 0.0 6 347 5 180 230 0.0 6 226 3 0 0 1 181 187 0.0 6 186 1 182 217 0.0 6 216 1 183 218 0.0 6 212 6 184 274 0.0 6 273 1 185 336 0.0 6 333 2 1 186 378 0.0 6 373 2 1 0 0 2 187 223 0.0 6 221 2 188 200 0.0 6 199 1 189 157 0.0 6 157 190 196 0.0 6 195 1 191 196 0.0 6 192 4 192 200 0.0 6 198 1 0 1 193 117 0.0 6 114 3 194 142 0.0 6 140 1 0 1 195 132 0.0 6 131 1 196 166 0.0 6 164 1 0 0 0 0 1 197 199 0.0 6 199 198 150 0.0 6 150 199 135 0.0 6 134 1 200 195 0.0 6 194 1 201 134 0.0 6 134 202 137 0.0 6 136 1 203 121 0.0 6 120 1 204 91 0.0 6 91 205 60 0.0 6 59 0 0 0 1 206 70 0.0 6 70 207 81 0.0 6 81 208 54 0.0 6 54 209 52 0.0 6 51 1 210 63 0.0 6 63 211 33 0.0 6 32 1 212 43 0.0 6 43 213 99 0.0 6 98 0 0 0 0 1 214 42 0.0 6 42 215 39 0.0 6 39 216 15 0.0 6 15 217 22 0.0 6 22 218 23 0.0 6 22 1 219 15 0.0 6 15 220 30 0.0 6 30 221 22 0.0 6 21 1 222 22 0.0 6 22 223 14 0.0 6 14 224 20 0.0 6 20 225 26 0.0 6 26 226 16 0.0 6 16 227 23 0.0 6 23 228 26 0.0 6 26 229 25 0.0 6 24 1 230 33 0.0 6 32 1 231 1 0.0 6 1 232 15 0.0 6 15 233 3 0.0 6 3 234 7 0.0 6 7 235 8 0.0 6 8 236 10 0.0 6 10 237 1 0.0 6 1 239 8 0.0 6 8 241 1 0.0 6 1 242 2 0.0 6 2 243 3 0.0 6 3 244 1 0.0 6 1 247 1 0.0 6 1 277 1 0.0 6 1 278 4 0.0 6 4 279 6 0.0 6 6 280 25 0.0 6 25 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 586 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Overview of removed sequences length count expect max.err error counts 70 3 0.0 6 3 71 11 0.0 6 11 72 4 0.0 6 4 73 1 0.0 6 1 74 22 0.0 6 22 75 3 0.0 6 3 76 4 0.0 6 4 77 4 0.0 6 4 78 7 0.0 6 7 79 1 0.0 6 1 80 3 0.0 6 3 81 5 0.0 6 5 82 13 0.0 6 12 0 0 0 1 83 1 0.0 6 1 84 8 0.0 6 8 85 1 0.0 6 1 86 1 0.0 6 1 89 8 0.0 6 7 0 0 0 1 90 2 0.0 6 2 91 1 0.0 6 1 92 3 0.0 6 3 93 1 0.0 6 1 94 2 0.0 6 2 95 5 0.0 6 5 96 2 0.0 6 2 99 3 0.0 6 2 0 0 0 1 100 11 0.0 6 11 101 27 0.0 6 27 102 1 0.0 6 1 104 3 0.0 6 2 1 105 15 0.0 6 13 0 0 0 2 106 32 0.0 6 32 107 1 0.0 6 1 109 2 0.0 6 2 110 3 0.0 6 3 111 3 0.0 6 3 112 2 0.0 6 2 113 1 0.0 6 1 116 4 0.0 6 4 122 1 0.0 6 1 123 1 0.0 6 1 127 4 0.0 6 4 128 1 0.0 6 1 130 5 0.0 6 5 131 15 0.0 6 15 132 36 0.0 6 35 0 0 0 1 133 37 0.0 6 37 134 4 0.0 6 4 135 1 0.0 6 1 136 1 0.0 6 0 0 0 0 1 137 2 0.0 6 0 0 0 0 2 141 2 0.0 6 1 0 0 0 1 142 4 0.0 6 4 143 11 0.0 6 11 144 1 0.0 6 1 147 3 0.0 6 3 148 17 0.0 6 17 152 6 0.0 6 5 0 0 0 1 157 2 0.0 6 2 158 3 0.0 6 3 159 9 0.0 6 9 160 50 0.0 6 50 161 7 0.0 6 6 0 0 0 1 162 1 0.0 6 1 163 10 0.0 6 10 164 1 0.0 6 0 1 167 4 0.0 6 4 168 12 0.0 6 12 169 2 0.0 6 2 171 1 0.0 6 1 172 15 0.0 6 15 173 5 0.0 6 5 175 2 0.0 6 2 176 4 0.0 6 4 179 2 0.0 6 2 180 9 0.0 6 9 181 35 0.0 6 34 1 182 1 0.0 6 1 183 2 0.0 6 2 184 1 0.0 6 1 185 3 0.0 6 3 193 1 0.0 6 1 196 1 0.0 6 1 197 1 0.0 6 1 198 5 0.0 6 5 210 1 0.0 6 1 211 5 0.0 6 5 212 1 0.0 6 1 215 1 0.0 6 0 0 0 0 1 228 2 0.0 6 2 229 5 0.0 6 5 237 2 0.0 6 2 238 4 0.0 6 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above. This is cutadapt 3.5 with Python 3.8.12 Command line parameters: --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_13_226_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_13_453_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_13_226_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_13_453_L001_R2_001.fastq.gz Processing reads on 16 cores in paired-end mode ... Finished in 0.37 s (123591 µs/read; 0.00 M reads/minute). === Summary === Total read pairs processed: 3 Read 1 with adapter: 2 (66.7%) Read 2 with adapter: 2 (66.7%) == Read fate breakdown == Pairs that were too short: 0 (0.0%) Pairs discarded as untrimmed: 1 (33.3%) Pairs written (passing filters): 2 (66.7%) Total basepairs processed: 1,226 bp Read 1: 713 bp Read 2: 513 bp Total written (filtered): 126 bp (10.3%) Read 1: 66 bp Read 2: 60 bp === First read: Adapter 1 === Sequence: ACGTCTGGTTCAGGGTTGTT; Type: regular 3'; Length: 20; Trimmed: 2 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 181 1 0.0 6 1 229 1 0.0 6 1 === First read: Adapter 2 === Sequence: AGCGGAGGAAAAGAAACTAA; Type: regular 5'; Length: 20; Trimmed: 0 times === Second read: Adapter 3 === Sequence: TTAGTTTCTTTTCCTCCGCT; Type: regular 3'; Length: 20; Trimmed: 2 times Minimum overlap: 3 No. of allowed errors: 1-2 bp: 0; 3-5 bp: 1; 6-9 bp: 2; 10-12 bp: 3; 13-15 bp: 4; 16-19 bp: 5; 20 bp: 6 Bases preceding removed adapters: A: 0.0% C: 100.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 113 1 0.0 6 1 187 1 0.0 6 1 === Second read: Adapter 4 === Sequence: AACAACCCTGAACCAGACGT; Type: regular 5'; Length: 20; Trimmed: 0 times cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_10_216_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_10_443_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_10_216_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_10_443_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_11_217_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_11_444_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_11_217_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_11_444_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_12_218_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_12_445_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_12_218_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_12_445_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_13_219_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_23-Rev_13_446_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_13_219_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_23-Rev_13_446_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_07_220_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_07_447_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_07_220_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_07_447_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_08_221_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_08_448_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_08_221_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_08_448_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_09_222_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_09_449_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_09_222_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_09_449_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_10_223_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_10_450_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_10_223_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_10_450_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_11_224_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_11_451_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_11_224_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_11_451_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_12_225_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_12_452_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_12_225_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_12_452_L001_R2_001.fastq.gz Command: cutadapt --cores 16 --error-rate 0.3 --times 1 --overlap 3 --minimum-length 1 -o /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_13_226_L001_R1_001.fastq.gz -p /tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-fp8y9znj/Fwd_24-Rev_13_453_L001_R2_001.fastq.gz --adapter ACGTCTGGTTCAGGGTTGTT --front AGCGGAGGAAAAGAAACTAA -A TTAGTTTCTTTTCCTCCGCT -G AACAACCCTGAACCAGACGT --match-read-wildcards --discard-untrimmed /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_13_226_L001_R1_001.fastq.gz /tmp/qiime2-archive-vdoa2l8_/593a3aba-3810-4ea4-8d7f-3a4d9f847ca9/data/Fwd_24-Rev_13_453_L001_R2_001.fastq.gz Saved SampleData[PairedEndSequencesWithQuality] to: CS_wf1/trimmed-demuxv1.qza