# \#unite **URL:** https://forum.qiime2.org/tag/unite/155.md [Latest](https://forum.qiime2.org/latest.md) · [Categories](https://forum.qiime2.org/categories.md) · [Tags](https://forum.qiime2.org/tags.md) --- ## [pre-trained UNITE ITS classifiers for QIIME 2026.10 and scikit-learn=1.7.1](https://forum.qiime2.org/t/pre-trained-unite-its-classifiers-for-qiime-2026-10-and-scikit-learn-1-7-1/24140)
**Author:** [@colinbrislawn](https://forum.qiime2.org/u/colinbrislawn)\ **Replies:** 15\ **Last updated:** [August 31, 2026, 6:59pm UTC](https://forum.qiime2.org/t/pre-trained-unite-its-classifiers-for-qiime-2026-10-and-scikit-learn-1-7-1/24140 "2026-08-31T18:59:19Z")
UNITE version 10.0 was updated on 2025-02-19, and I've built classifiers for it compatible with the newest version of Qiime2: New in 2026 Runs the qiime2-workshop:2026.10 image or Pixi on OSX I've rewritten the pipeli… --- ## [No "Unclassified" etc taxa detected with grep in multiple unfiltered UNITE dbs?](https://forum.qiime2.org/t/no-unclassified-etc-taxa-detected-with-grep-in-multiple-unfiltered-unite-dbs/33917)
**Author:** [@sibilant](https://forum.qiime2.org/u/sibilant)\ **Replies:** 1\ **Last updated:** [January 19, 2026, 12:12am UTC](https://forum.qiime2.org/t/no-unclassified-etc-taxa-detected-with-grep-in-multiple-unfiltered-unite-dbs/33917 "2026-01-19T00:12:48Z")
Hi Qiime2 community, :qiime2: :hot\_beverage: I am new to qiime2 and ITS analysis. I am running qiime2-amplicon-2025.7 in conda. Following recommendations on the qiime2 forum, I chose the developer version of the latest… --- ## [Issue with UNITE classifier for ITS1](https://forum.qiime2.org/t/issue-with-unite-classifier-for-its1/33837)
**Author:** [@mhog](https://forum.qiime2.org/u/mhog)\ **Replies:** 1\ **Last updated:** [December 2, 2025, 4:25pm UTC](https://forum.qiime2.org/t/issue-with-unite-classifier-for-its1/33837 "2025-12-02T16:25:54Z")
Hi there, I’m trying to assign taxonomy to my fungal ITS1 data using a pre-trained UNITE classifier. I’m using QIIME 2 version 2025.10 and conda (miniconda). I was having issues running ITSxpress with my paired-end data… --- ## [Evaluate custom taxonomic classifier with RESCRIPt?](https://forum.qiime2.org/t/evaluate-custom-taxonomic-classifier-with-rescript/33813)
**Author:** [@sibilant](https://forum.qiime2.org/u/sibilant)\ **Replies:** 5\ **Last updated:** [November 27, 2025, 5:41am UTC](https://forum.qiime2.org/t/evaluate-custom-taxonomic-classifier-with-rescript/33813 "2025-11-27T05:41:23Z")
Greetings Q2 and RESCRIPt community, :globe\_showing\_europe\_africa: I am using qiime2-amplicon-2025.7, and am new to qiime2. I am analyzing ITS1 amplicon metagenomic data from MiSeq Illumina runs. I am benchmarking my p… --- ## ["Killed" Error When Running classify-sklearn in QIIME 2](https://forum.qiime2.org/t/killed-error-when-running-classify-sklearn-in-qiime-2/32760)
**Author:** [@Percy](https://forum.qiime2.org/u/Percy)\ **Replies:** 8\ **Last updated:** [March 23, 2025, 12:42pm UTC](https://forum.qiime2.org/t/killed-error-when-running-classify-sklearn-in-qiime-2/32760 "2025-03-23T12:42:27Z")
Hi everyone, I’ve been encountering an issue with QIIME 2 while running the classify-sklearn command for taxonomy classification, and I’m hoping someone can help me figure this out. Thanks for your kindness for helping … --- ## [How to train a UNITE classifier using RESCRIPt](https://forum.qiime2.org/t/how-to-train-a-unite-classifier-using-rescript/28285)
**Author:** [@colinbrislawn](https://forum.qiime2.org/u/colinbrislawn)\ **Replies:** 8\ **Last updated:** [January 3, 2025, 2:37pm UTC](https://forum.qiime2.org/t/how-to-train-a-unite-classifier-using-rescript/28285 "2025-01-03T14:37:03Z")
:construction: This tutorial is a work in progress. Please leave us questions and comments so we can better help you use this database. All feedback is welcome. If you are looking for a pre-trained ITS classifier, you c… --- ## [Taxa collapse shows too general taxonomic assignations](https://forum.qiime2.org/t/taxa-collapse-shows-too-general-taxonomic-assignations/30526)
**Author:** [@salias](https://forum.qiime2.org/u/salias)\ **Replies:** 11\ **Last updated:** [June 20, 2024, 2:20pm UTC](https://forum.qiime2.org/t/taxa-collapse-shows-too-general-taxonomic-assignations/30526 "2024-06-20T14:20:18Z")
I'm collapsing my feature table by family taxonomic level prior to running ANCOM-BC. I generated the QZV from the QZA collapsed table, and I found that there is a too general taxonomic assignation with a really high frec… --- ## [Issues with taxonomic classification UNITE 9.0 and 10.0](https://forum.qiime2.org/t/issues-with-taxonomic-classification-unite-9-0-and-10-0/30402)
**Author:** [@mica.tosi](https://forum.qiime2.org/u/mica.tosi)\ **Replies:** 2\ **Last updated:** [June 6, 2024, 8:19am UTC](https://forum.qiime2.org/t/issues-with-taxonomic-classification-unite-9-0-and-10-0/30402 "2024-06-06T08:19:48Z")
Hello QIIME2 team :wave: We wanted to share some issues we’ve been having with recent UNITE classifiers for fungal ITS. We found someone with a similar problem in a closed discussion, but unfortunately that information … --- ## [Which version of UNITE database should I choose?](https://forum.qiime2.org/t/which-version-of-unite-database-should-i-choose/12049)
**Author:** [@sixvable](https://forum.qiime2.org/u/sixvable)\ **Replies:** 2\ **Last updated:** [February 23, 2024, 8:03pm UTC](https://forum.qiime2.org/t/which-version-of-unite-database-should-i-choose/12049 "2024-02-23T20:03:28Z")
Recently I have been trying to analysis my ITS amplicon sequences data and I found a little bit confusing thing about the Unite database. On the Unite database they have distributed two different type of fungi unite dat… --- ## [UNITE, Include certain species of fungi](https://forum.qiime2.org/t/unite-include-certain-species-of-fungi/28800)
**Author:** [@dayana\_guaman](https://forum.qiime2.org/u/dayana_guaman)\ **Replies:** 1\ **Last updated:** [January 4, 2024, 4:53pm UTC](https://forum.qiime2.org/t/unite-include-certain-species-of-fungi/28800 "2024-01-04T16:53:49Z")
hello to all, I would like to know how would be the command to include a certain species of fungi with which I want to work, I tried but it tells me that the SampleID of the sequences does not match. --- ## [Optimizing taxonomy assignment to reduce unassigned ITS reads](https://forum.qiime2.org/t/optimizing-taxonomy-assignment-to-reduce-unassigned-its-reads/22249)
**Author:** [@Sonja](https://forum.qiime2.org/u/Sonja)\ **Replies:** 3\ **Last updated:** [February 22, 2022, 11:40pm UTC](https://forum.qiime2.org/t/optimizing-taxonomy-assignment-to-reduce-unassigned-its-reads/22249 "2022-02-22T23:40:09Z")
Hello everyone, I'm analysing microbial communities in a fjord and got stuck with the taxonomy assignment of fungi, I get 25-90% unassigned reads in my samples. The amplicons were generated using ITS3 and ITS4 as primer… --- ## [Interpreting Unassigned/k\_Fungi ITS taxonomy generated using 3 reference datasets (UNITE & NCBI)](https://forum.qiime2.org/t/interpreting-unassigned-k-fungi-its-taxonomy-generated-using-3-reference-datasets-unite-ncbi/22202)
**Author:** [@ardorbel](https://forum.qiime2.org/u/ardorbel)\ **Replies:** 1\ **Last updated:** [February 17, 2022, 8:00am UTC](https://forum.qiime2.org/t/interpreting-unassigned-k-fungi-its-taxonomy-generated-using-3-reference-datasets-unite-ncbi/22202 "2022-02-17T08:00:58Z")
Hello, I'm currently comparing taxonomy results for ITS1 sequences derived from sediment samples (amplified using ITS1F/ITS2), obtained using three different naive-bayes classifiers (trained in qiime2) using the followi… --- ## [feature-classifier error while using UNITE](https://forum.qiime2.org/t/feature-classifier-error-while-using-unite/22151)
**Author:** [@April\_Oliver](https://forum.qiime2.org/u/April_Oliver)\ **Replies:** 5\ **Last updated:** [February 11, 2022, 9:04pm UTC](https://forum.qiime2.org/t/feature-classifier-error-while-using-unite/22151 "2022-02-11T21:04:19Z")
I'm struggling to assign taxonomy to ITS amplicons. I downloaded the UNITE v8 database and imported the reference sequences and taxonomy using the following: qiime tools import \\ --type FeatureData\[Sequence\] \\ --i… --- ## [Taxonomic assignment with UNITE database: most reads classified as "s\_\_unidentified"](https://forum.qiime2.org/t/taxonomic-assignment-with-unite-database-most-reads-classified-as-s-unidentified/18696)
**Author:** [@Danilo\_Reis](https://forum.qiime2.org/u/Danilo_Reis)\ **Replies:** 6\ **Last updated:** [March 10, 2021, 10:19am UTC](https://forum.qiime2.org/t/taxonomic-assignment-with-unite-database-most-reads-classified-as-s-unidentified/18696 "2021-03-10T10:19:09Z")
Hi everyone, I am for the first time using Qiime2 to analyze ITS data (Illumina Miseq V3 - 300 bp paired-end reads) for fungal endophytes diversity in plant roots. I received different folders from the sequencing facil… --- ## [Importing Unite 8.2 version (2020) from a single file](https://forum.qiime2.org/t/importing-unite-8-2-version-2020-from-a-single-file/16907)
**Author:** [@Marisa\_Tello\_Martin](https://forum.qiime2.org/u/Marisa_Tello_Martin)\ **Replies:** 2\ **Last updated:** [October 6, 2020, 8:19pm UTC](https://forum.qiime2.org/t/importing-unite-8-2-version-2020-from-a-single-file/16907 "2020-10-06T20:19:53Z")
Hi Colin, I have download the Unite 8.2 version (2020) from these 4 different releases and I get the same problem that Nicholas had. It is just one single file instead the fasta and txt file that is provided in the 8.0 … --- ## [ITS classification - nothing beyond phylum level](https://forum.qiime2.org/t/its-classification-nothing-beyond-phylum-level/15022)
**Author:** [@spongebob](https://forum.qiime2.org/u/spongebob)\ **Replies:** 6\ **Last updated:** [June 6, 2020, 2:20am UTC](https://forum.qiime2.org/t/its-classification-nothing-beyond-phylum-level/15022 "2020-06-06T02:20:16Z")
Hi there, I recently migrated over to qiime2 from mothur. I am processing the same ITS sequence data, that i did with mothur and i am using the same UNITE database for ITS classification. When i used the mothur pipeli… --- ## [Issue running commands on cluster via srun](https://forum.qiime2.org/t/issue-running-commands-on-cluster-via-srun/13833)
**Author:** [@Mr\_Burns](https://forum.qiime2.org/u/Mr_Burns)\ **Replies:** 3\ **Last updated:** [March 2, 2020, 10:56pm UTC](https://forum.qiime2.org/t/issue-running-commands-on-cluster-via-srun/13833 "2020-03-02T22:56:04Z")
Hello, i am running qiime2 on conda (HPC), analyzing ITS fungi. everything is fine until the Assign taxonomy command: qiime feature-classifier classify-sklearn --i-classifier unite-ver8-99-classifier.qza --i-reads … --- ## [Full UNITE+INSD dataset for Fungi](https://forum.qiime2.org/t/full-unite-insd-dataset-for-fungi/13409)
**Author:** [@shashankgpt](https://forum.qiime2.org/u/shashankgpt)\ **Replies:** 1\ **Last updated:** [February 3, 2020, 3:33pm UTC](https://forum.qiime2.org/t/full-unite-insd-dataset-for-fungi/13409 "2020-02-03T15:33:29Z")
Hi, I was wondering about which database should we use for ITS sequence analysis, UNITE or UNITE+INSD dataset? Is the UNITE+INSD dataset stable? Secondly, I got an error while training the UNITE database qiime tools … --- ## [The space in line header of UNITE database](https://forum.qiime2.org/t/the-space-in-line-header-of-unite-database/12807)
**Author:** [@ZhengQu](https://forum.qiime2.org/u/ZhengQu)\ **Replies:** 1\ **Last updated:** [December 10, 2019, 4:53pm UTC](https://forum.qiime2.org/t/the-space-in-line-header-of-unite-database/12807 "2019-12-10T16:53:24Z")
Dear QIIME2 users When I used the UNITE database （QIIME release for Fungi. Version 18.11.2018.) to analyze and after I modified by the code（See below\*），I found a space in line 67868 header. Although there is only one s… --- ## [Training a new UNITE fungal classifier](https://forum.qiime2.org/t/training-a-new-unite-fungal-classifier/12070)
**Author:** [@hesongbing](https://forum.qiime2.org/u/hesongbing)\ **Replies:** 5\ **Last updated:** [November 4, 2019, 8:51am UTC](https://forum.qiime2.org/t/training-a-new-unite-fungal-classifier/12070 "2019-11-04T08:51:57Z")
Hi @Sydney\_Morgan, thanks for sharing your own classifier! But I met several problems when using this classifier. My command: qiime feature-classifier classify-sklearn –i-classifier unite-ver8-99-classifier.qza –i-r… --- ## [Failed to download data from UNITE](https://forum.qiime2.org/t/failed-to-download-data-from-unite/12278)
**Author:** [@hesongbing](https://forum.qiime2.org/u/hesongbing)\ **Replies:** 5\ **Last updated:** [November 4, 2019, 12:41am UTC](https://forum.qiime2.org/t/failed-to-download-data-from-unite/12278 "2019-11-04T00:41:17Z")
Dear All, Can anyone help me get over this trouble? I failed to download data from UNITE(UNITE - Resources). Can anyone download the QIIME files released at UNITE - Resources for me so that I can train UNITE fungal cla… --- ## [UNITE (v.8.0 2018-11-18) Classifiers for QIIME2 Available Here](https://forum.qiime2.org/t/unite-v-8-0-2018-11-18-classifiers-for-qiime2-available-here/8750)
**Author:** [@Sydney\_Morgan](https://forum.qiime2.org/u/Sydney_Morgan)\ **Replies:** 3\ **Last updated:** [July 23, 2019, 10:30am UTC](https://forum.qiime2.org/t/unite-v-8-0-2018-11-18-classifiers-for-qiime2-available-here/8750 "2019-07-23T10:30:01Z")
Hello everyone, I have created two new UNITE fungal classifiers for QIIME2 that I'd like to share with you, since the classifiers that I posted previously (UNITE (ver. 7.2) Classifiers) use an old version of UNITE and a… --- ## [UNITE error in Developer DB](https://forum.qiime2.org/t/unite-error-in-developer-db/11756)
**Author:** [@Dichrome\_Viot](https://forum.qiime2.org/u/Dichrome_Viot)\ **Replies:** 1\ **Last updated:** [September 30, 2019, 1:17pm UTC](https://forum.qiime2.org/t/unite-error-in-developer-db/11756 "2019-09-30T13:17:24Z")
I have met that problem in the latest developer version(version 8, 02.02.2019) of the UNITE database rather than the formal version. I cannot figure this problem out using awk or sed so it may be a new problem. --- ## [Unite dynamic classifier](https://forum.qiime2.org/t/unite-dynamic-classifier/10157)
**Author:** [@Esteban\_Ceriani](https://forum.qiime2.org/u/Esteban_Ceriani)\ **Replies:** 2\ **Last updated:** [June 11, 2019, 12:58am UTC](https://forum.qiime2.org/t/unite-dynamic-classifier/10157 "2019-06-11T00:58:53Z")
Hi to all! I have the latest release of QIIME2+Virtualbox (2019.4+6.0) and downloaded the UNITE classifiers from Link. When I try to use the dynamic classifier I got this message: qiime feature-classifier classify-skle… --- ## [Accession number for dataset](https://forum.qiime2.org/t/accession-number-for-dataset/7747)
**Author:** [@Fabs](https://forum.qiime2.org/u/Fabs)\ **Replies:** 1\ **Last updated:** [January 9, 2019, 3:06pm UTC](https://forum.qiime2.org/t/accession-number-for-dataset/7747 "2019-01-09T15:06:10Z")
Hi, I have a quick question. I have already done all my processing and I must say, I could not have fully completed my work without this forum. So thank you for that. My question is, I have the taxonomy but I am getting… --- ## [Choosing UNITE release for classifier training](https://forum.qiime2.org/t/choosing-unite-release-for-classifier-training/6743)
**Author:** [@mdziurzynski](https://forum.qiime2.org/u/mdziurzynski)\ **Replies:** 3\ **Last updated:** [November 3, 2018, 4:46pm UTC](https://forum.qiime2.org/t/choosing-unite-release-for-classifier-training/6743 "2018-11-03T16:46:20Z")
Hello, I am trying to correctly assign taxonomy of my ITS2 sequences using UNITE database. I would like to train my own classifier, but I don't know which version of the UNITE database should I use, should I use: data… --- ## [Combine different reference sequences databases](https://forum.qiime2.org/t/combine-different-reference-sequences-databases/2853)
**Author:** [@jacorvar](https://forum.qiime2.org/u/jacorvar)\ **Replies:** 1\ **Last updated:** [February 3, 2018, 2:53pm UTC](https://forum.qiime2.org/t/combine-different-reference-sequences-databases/2853 "2018-02-03T14:53:07Z")
Hi I'm a newbie with QIIME2 and would like to analyse the diversity of a microbiome. In my particular case, I am not only interested in bacteria, but also in fungii and other microorganisms. For this reason, I merged the…