# \#qiime2-20197

**URL:** https://forum.qiime2.org/tag/qiime2-20197/196.md

[Latest](https://forum.qiime2.org/latest.md) · [Categories](https://forum.qiime2.org/categories.md) · [Tags](https://forum.qiime2.org/tags.md)

---

## [demux is killed in version 2019.7, but not in 2019.1](https://forum.qiime2.org/t/demux-is-killed-in-version-2019-7-but-not-in-2019-1/11317)

<div class="topic-metadata">

**Author:** [@Anne\_Ballou](https://forum.qiime2.org/u/Anne_Ballou)\
**Replies:** 9\
**Last updated:** [August 11, 2020, 6:33pm UTC](https://forum.qiime2.org/t/demux-is-killed-in-version-2019-7-but-not-in-2019-1/11317 "2020-08-11T18:33:06Z")

</div>

Hi, I've been trying to demux a dataset with 2019.7, but it seems to demand more memory than demux in 2019.1, and either takes a ridiculously long time, or the kernal kills the process. I've tried on both a linux laptop…

---

## [SILVA 132 Classifiers 341f-806r region](https://forum.qiime2.org/t/silva-132-classifiers-341f-806r-region/11270)

<div class="topic-metadata">

**Author:** [@Micro\_Biologist](https://forum.qiime2.org/u/Micro_Biologist)\
**Replies:** 7\
**Last updated:** [December 17, 2019, 5:19am UTC](https://forum.qiime2.org/t/silva-132-classifiers-341f-806r-region/11270 "2019-12-17T05:19:28Z")

</div>

I couldn't find classifiers for this region, so I used code available here to train new ones, and thought I might as well share them for all to use. Here is the new version for using primers: f\_primer = 'CCTACGGGNGGCWG…

---

## [Qiime2 trimming after join-pairs](https://forum.qiime2.org/t/qiime2-trimming-after-join-pairs/12262)

<div class="topic-metadata">

**Author:** [@Morena\_Cau](https://forum.qiime2.org/u/Morena_Cau)\
**Replies:** 1\
**Last updated:** [October 31, 2019, 12:10pm UTC](https://forum.qiime2.org/t/qiime2-trimming-after-join-pairs/12262 "2019-10-31T12:10:12Z")

</div>

Goodmorning all! My question is: I done the qiime vsearch join-pairs \\ command to join my forward and reverse reads (produced by Miseq) and this is the result. Which criteria should I use to trim it? Do I have to u…
