# \#ncbi
**URL:** https://forum.qiime2.org/tag/ncbi/236.md
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## [Difficulty assigning taxonomy to cyanobacterial ASVs](https://forum.qiime2.org/t/difficulty-assigning-taxonomy-to-cyanobacterial-asvs/33226)
**Author:** [@Mahima\_y](https://forum.qiime2.org/u/Mahima_y)\
**Replies:** 2\
**Last updated:** [June 5, 2025, 5:48pm UTC](https://forum.qiime2.org/t/difficulty-assigning-taxonomy-to-cyanobacterial-asvs/33226 "2025-06-05T17:48:24Z")
Hello! I am trying to find the best method and database to assign taxonomy to my 1,107 ASVs. (For context, my samples were collected from seawater that was filtered and prepared for sequencing, bioinformatics, etc). Beca…
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## [mismatch between taxonomy and sequence data that download from NCBI using qiime rescript get-ncbi-data](https://forum.qiime2.org/t/mismatch-between-taxonomy-and-sequence-data-that-download-from-ncbi-using-qiime-rescript-get-ncbi-data/33130)
**Author:** [@Si\_Vivy](https://forum.qiime2.org/u/Si_Vivy)\
**Replies:** 3\
**Last updated:** [May 19, 2025, 7:58am UTC](https://forum.qiime2.org/t/mismatch-between-taxonomy-and-sequence-data-that-download-from-ncbi-using-qiime-rescript-get-ncbi-data/33130 "2025-05-19T07:58:44Z")
Hello ! I am trying to download COI data from NCBI with this code: qiime rescript get-ncbi-data \\ --p-query '(cytochrome c oxidase subunit I\[gene\] OR cytochrome oxidase subunit 1\[gene\] OR cytochrome oxidase subunit I\[…
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## [Database With Best Accuracy at Genus Level for Human Microbiome?](https://forum.qiime2.org/t/database-with-best-accuracy-at-genus-level-for-human-microbiome/31188)
**Author:** [@pone](https://forum.qiime2.org/u/pone)\
**Replies:** 8\
**Last updated:** [August 28, 2024, 7:01pm UTC](https://forum.qiime2.org/t/database-with-best-accuracy-at-genus-level-for-human-microbiome/31188 "2024-08-28T19:01:37Z")
If you have an Illumina FASTQ corresponding to a human stool sample, which genomics databases and bioinformatics pathway(s) will give you the best accuracy at the genus level? Is there any research that ranks the datab…
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## [Reconciling 16s Databases in a Single Pipeline](https://forum.qiime2.org/t/reconciling-16s-databases-in-a-single-pipeline/31258)
**Author:** [@pone](https://forum.qiime2.org/u/pone)\
**Replies:** 5\
**Last updated:** [August 28, 2024, 3:36pm UTC](https://forum.qiime2.org/t/reconciling-16s-databases-in-a-single-pipeline/31258 "2024-08-28T15:36:18Z")
Is it possible to create a pipeline for 16s sequences that would try to reconcile sequence assignments across multiple databases? The point of this is to expose ambiguities and different assignments explicitly within …
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## [getting NCBI ID problem](https://forum.qiime2.org/t/getting-ncbi-id-problem/28068)
**Author:** [@kubra](https://forum.qiime2.org/u/kubra)\
**Replies:** 5\
**Last updated:** [October 25, 2023, 6:07pm UTC](https://forum.qiime2.org/t/getting-ncbi-id-problem/28068 "2023-10-25T18:07:43Z")
Hi, I have completed microbiome analyses of my samples. I will use the abundance of the microorganisms to train my project. However I have to use microorganisms' NCBI IDs instead of their names in my project. Is there a …
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## [how to compare SILVA 138 taxonomy and NCBI taxonomy](https://forum.qiime2.org/t/how-to-compare-silva-138-taxonomy-and-ncbi-taxonomy/27414)
**Author:** [@Wei\_Zhang](https://forum.qiime2.org/u/Wei_Zhang)\
**Replies:** 3\
**Last updated:** [August 22, 2023, 5:23pm UTC](https://forum.qiime2.org/t/how-to-compare-silva-138-taxonomy-and-ncbi-taxonomy/27414 "2023-08-22T17:23:20Z")
Hi there, I have two datasets, 16S rRNA data that was annotated with SILVA taxonomy, and shotgun data that was annotated with NCBI taxonomy. I want to compare the difference and find common taxa between the two datasets…
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## [q2-fondue: Plugin for (meta)data retrieval from SRA](https://forum.qiime2.org/t/q2-fondue-plugin-for-meta-data-retrieval-from-sra/22595)
**Author:** [@misialq](https://forum.qiime2.org/u/misialq)\
**Replies:** 0\
**Last updated:** [March 28, 2022, 7:34am UTC](https://forum.qiime2.org/t/q2-fondue-plugin-for-meta-data-retrieval-from-sra/22595 "2022-03-28T07:34:00Z")
q2-fondue: (meta)data retrieval from SRA made simple Hello QIIMErs! On behalf of the Bokulich Lab team I would like to present to you our brand new plugin: q2-fondue (Functions for reproducibly Obtaining and Normalizing…