# \#muscle

**URL:** https://forum.qiime2.org/tag/muscle/55.md

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## [Which alignment algorithm should I use](https://forum.qiime2.org/t/which-alignment-algorithm-should-i-use/997)

<div class="topic-metadata">

**Author:** [@lindd](https://forum.qiime2.org/u/lindd)\
**Replies:** 1\
**Last updated:** [August 14, 2017, 3:03pm UTC](https://forum.qiime2.org/t/which-alignment-algorithm-should-i-use/997 "2017-08-14T15:03:22Z")

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Hi, I am a beginner of microbiome data preprocessing and qiime2. I have done the quality control by DADA2 and want to build a phylogenetic tree to calculate diversity. According to moving picture tutorial, I need to ali…