# \#hpc

**URL:** https://forum.qiime2.org/tag/hpc/124.md

[Latest](https://forum.qiime2.org/latest.md) · [Categories](https://forum.qiime2.org/categories.md) · [Tags](https://forum.qiime2.org/tags.md)

---

## [DADA2 fails silently on SLURM cluster (QIIME2 2024.10) — No .qza outputs, no error messages](https://forum.qiime2.org/t/dada2-fails-silently-on-slurm-cluster-qiime2-2024-10-no-qza-outputs-no-error-messages/33186)

<div class="topic-metadata">

**Author:** [@Katherine-KMC](https://forum.qiime2.org/u/Katherine-KMC)\
**Replies:** 1\
**Last updated:** [May 22, 2025, 9:30pm UTC](https://forum.qiime2.org/t/dada2-fails-silently-on-slurm-cluster-qiime2-2024-10-no-qza-outputs-no-error-messages/33186 "2025-05-22T21:30:15Z")

</div>

Context Hi QIIME2 team, I'm running QIIME2 2024.10 via a SLURM-managed cluster (HPC from PUCV Chile), using Miniconda with a validated environment. I’m trying to run qiime dada2 denoise-paired on a validated .qza file …

---

## [Question about Docker build and installation location](https://forum.qiime2.org/t/question-about-docker-build-and-installation-location/28930)

<div class="topic-metadata">

**Author:** [@cjfields](https://forum.qiime2.org/u/cjfields)\
**Replies:** 6\
**Last updated:** [May 15, 2025, 5:18pm UTC](https://forum.qiime2.org/t/question-about-docker-build-and-installation-location/28930 "2025-05-15T17:18:25Z")

</div>

I wanted to ask a general question about the Docker image setup that is currently used for QIIME2, as we've seen issues with this on our local HPC when running this under Singularity, and may affect portability of workfl…

---

## [Conflicts found! Workaround by installing Qiime2 without .yml?](https://forum.qiime2.org/t/conflicts-found-workaround-by-installing-qiime2-without-yml/26839)

<div class="topic-metadata">

**Author:** [@cckeneally](https://forum.qiime2.org/u/cckeneally)\
**Replies:** 2\
**Last updated:** [June 21, 2023, 5:37am UTC](https://forum.qiime2.org/t/conflicts-found-workaround-by-installing-qiime2-without-yml/26839 "2023-06-21T05:37:41Z")

</div>

Hi, I'm trying to install the latest version of Qiime2 as a user on an HPC (I don't have write permissions to base conda to update it or run a clean install, which seems to fix this issue for others having similar conda…

---

## [install issue for 2023.2 on HPC](https://forum.qiime2.org/t/install-issue-for-2023-2-on-hpc/25787)

<div class="topic-metadata">

**Author:** [@Scarleth\_Bravo](https://forum.qiime2.org/u/Scarleth_Bravo)\
**Replies:** 1\
**Last updated:** [March 14, 2023, 5:12pm UTC](https://forum.qiime2.org/t/install-issue-for-2023-2-on-hpc/25787 "2023-03-14T17:12:48Z")

</div>

Hello, I have the same problem. My PC has Centos 8 as operating system. I installed the program with conda Qiime2 environment, version 2023.2. I was able to activate the environment and it threw me this error: QIIME i…

---

## [issue installing QIIME 2 2022.11 on HPC server](https://forum.qiime2.org/t/issue-installing-qiime-2-2022-11-on-hpc-server/25692)

<div class="topic-metadata">

**Author:** [@asajoh](https://forum.qiime2.org/u/asajoh)\
**Replies:** 0\
**Last updated:** [February 20, 2023, 3:38pm UTC](https://forum.qiime2.org/t/issue-installing-qiime-2-2022-11-on-hpc-server/25692 "2023-02-20T15:38:16Z")

</div>

Hi! We are experiencing the same problem, so instead of creating a new thread, I thought we might contribute to this one with the information requested for our system. We have installed qiime2-2022.11 on a virtual mach…

---

## [Estimating time and memory for masking large dataset](https://forum.qiime2.org/t/estimating-time-and-memory-for-masking-large-dataset/21166)

<div class="topic-metadata">

**Author:** [@BeckWehrle](https://forum.qiime2.org/u/BeckWehrle)\
**Replies:** 11\
**Last updated:** [November 20, 2021, 9:04am UTC](https://forum.qiime2.org/t/estimating-time-and-memory-for-masking-large-dataset/21166 "2021-11-20T09:04:00Z")

</div>

I want to mask my very large (\>1.5 million sequences) aligned\_rep\_seqsDNA.qza data. I performed the alignment with SINA 1.7.2 using SILVA as a reference database, and imported the FASTA output into QIIME2. The file is va…

---

## [Permission error in home directory. How to redirect .config to another folder on HPC?](https://forum.qiime2.org/t/permission-error-in-home-directory-how-to-redirect-config-to-another-folder-on-hpc/19802)

<div class="topic-metadata">

**Author:** [@eskavvas](https://forum.qiime2.org/u/eskavvas)\
**Replies:** 3\
**Last updated:** [June 4, 2021, 7:25pm UTC](https://forum.qiime2.org/t/permission-error-in-home-directory-how-to-redirect-config-to-another-folder-on-hpc/19802 "2021-06-04T19:25:45Z")

</div>

Hi, I'm trying to run qiime2 on a HPC cluster but I'm running into a permission error in my home directory. I contacted the administrator and he recommended that I redirect the location of .config to the /share folder i…

---

## [headless/HPC use of QIIME](https://forum.qiime2.org/t/headless-hpc-use-of-qiime/2587)

<div class="topic-metadata">

**Author:** [@Cistron](https://forum.qiime2.org/u/Cistron)\
**Replies:** 4\
**Last updated:** [September 27, 2019, 11:49am UTC](https://forum.qiime2.org/t/headless-hpc-use-of-qiime/2587 "2019-09-27T11:49:17Z")

</div>

Hello everybody, First off: Thanks for developing QIIME! I have a few questions and points regarding headless use of QIIME. For the last two years I've been using QIIME1 and I have only dabbled superficially with QIIM…

---

## [Threads vs Nodes](https://forum.qiime2.org/t/threads-vs-nodes/11323)

<div class="topic-metadata">

**Author:** [@jwdebelius](https://forum.qiime2.org/u/jwdebelius)\
**Replies:** 3\
**Last updated:** [August 30, 2019, 9:46pm UTC](https://forum.qiime2.org/t/threads-vs-nodes/11323 "2019-08-30T21:46:57Z")

</div>

Hi All, I'm trying to understand the difference between the --p-n-jobs and --p-threads (for instance, comparing qiime feature-classifier classify-sklearn and qiime fragment-insertion sepp). When I spin them on a server,…

---

## [Working in parallel on a cluster](https://forum.qiime2.org/t/working-in-parallel-on-a-cluster/1658)

<div class="topic-metadata">

**Author:** [@nir](https://forum.qiime2.org/u/nir)\
**Replies:** 3\
**Last updated:** [November 2, 2017, 7:58pm UTC](https://forum.qiime2.org/t/working-in-parallel-on-a-cluster/1658 "2017-11-02T19:58:42Z")

</div>

hi, i am working on an SGE cluster, how do i set up working in parallel? i see some scripts have an option to set the number of threads, but i am not sure how to submit the jobs to a queue.
