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Sequencing platform fully confounded with study period: MiSeq vs NextSeq 2000 (16S rRNA V3-V4)
|
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3
|
54
|
July 7, 2026
|
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QIIME 2 processing of MiSeq i100 data with constant quality scores
|
|
3
|
94
|
June 8, 2026
|
|
Running DADA2 on pre-processed data
|
|
7
|
95
|
June 3, 2026
|
|
Query about false read merging or no read merging
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|
1
|
37
|
May 26, 2026
|
|
Merging NGS with TGS?
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3
|
57
|
May 20, 2026
|
|
Under the hood of the "Moving Pictures" tutorial
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0
|
133
|
May 13, 2026
|
|
sequence alignment
|
|
5
|
69
|
May 11, 2026
|
|
An error was encountered while running DADA2 in R (return code 1), inspect stdout and stderr to learn more
|
|
10
|
115
|
May 8, 2026
|
|
Help - DADA2 microbiome analysis
|
|
3
|
55
|
May 4, 2026
|
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Qiime version and singletons
|
|
3
|
72
|
April 27, 2026
|
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High number of unique sequences in PacBio full-length 16S CCS data with DADA2
|
|
2
|
106
|
April 24, 2026
|
|
Low overlap due to poor reverse read quality in 2×300 bp V3–V4 amplicon sequencing (DADA2)
|
|
1
|
63
|
April 13, 2026
|
|
High but variable sequence loss (32-68%) during DADA2 filtering with V3-V4 amplicons (MiSeq 600 cycles)
|
|
2
|
105
|
April 9, 2026
|
|
Inadequate overlap for V3-V4 (paired-end) due to quality drop
|
|
5
|
112
|
March 9, 2026
|
|
Integrating V3-V4 and V4 16S datasets for large-scale meta-analysis: Is Greengenes2 the optimal choice for cross-region alignment?
|
|
1
|
140
|
March 6, 2026
|
|
Too many elements for DADA2 - denoise in batches?
|
|
6
|
139
|
March 4, 2026
|
|
Analysis with DADA2... Merge Read Length
|
|
1
|
52
|
March 4, 2026
|
|
denoise failure
|
|
5
|
72
|
February 24, 2026
|
|
Huge difference of DADA2 output with and without removing primers
|
|
4
|
107
|
January 27, 2026
|
|
Advice in best practice for handling multiple sequencing runs and subset analyses in QIIME 2 (DADA2)
|
|
2
|
94
|
January 16, 2026
|
|
pair ended demultiplexed qza file causing strange error.
|
|
1
|
60
|
December 28, 2025
|
|
High read loss due to artifacts and chimera after denoising 16S V3-V4 paired-end reads
|
|
1
|
66
|
December 17, 2025
|
|
Denoising low quality reverse reads in paired-end sequencing reads
|
|
4
|
89
|
December 3, 2025
|
|
Issue with UNITE classifier for ITS1
|
|
1
|
81
|
December 2, 2025
|
|
Error was encountered while running DADA2 in R (return code 1)
|
|
6
|
103
|
December 1, 2025
|
|
DADA2 denoising taking long time
|
|
6
|
136
|
December 1, 2025
|
|
Questions about performing quality control with dada2 and generating a feature table
|
|
2
|
53
|
November 29, 2025
|
|
DADA2 priors trimming
|
|
1
|
36
|
November 18, 2025
|
|
priors in qiime2-dada2
|
|
3
|
265
|
April 25, 2023
|
|
QIIME2 non-chimeric reads
|
|
3
|
88
|
November 10, 2025
|
