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Significant 16S sequence loss while merging w/ DADA2, various truncation settings used
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3
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121
|
December 6, 2025
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DADA2 Fails on AVITI Data
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8
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209
|
November 29, 2025
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qiime dada2 denoise-paired is unresponsive
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3
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65
|
November 27, 2025
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Interpreting KEGG vs MetaCyc pathways in 16S (PICRUSt2) functional prediction
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1
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168
|
October 18, 2025
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Observed ASV richness drops after adding 3rd MiSeq library — why do I lose ~100 species when I merge libraries?
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4
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41
|
November 16, 2025
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ITS denoising truncation
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2
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63
|
November 15, 2025
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Need help choosing truncation lengths for DADA2 and interpreting the demultiplexed sequence count summary.
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2
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50
|
November 13, 2025
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Converting 16S V3–V4 ASV reads to absolute copy numbers (qPCR / spike-in calibration?)
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3
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118
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October 9, 2025
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Chimera removal dilemma in 16S V3–V4: necessary when using Kraken2/Bracken?
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8
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183
|
September 26, 2025
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Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis?
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4
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181
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September 18, 2025
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Trained classifier output not found
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7
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114
|
October 13, 2025
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16S CCS without primers: how to skip --p-front/--p-adapter in denoise-ccs?
|
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4
|
106
|
September 26, 2025
|
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Percentage of reads discarded after running DADA2 on NextSeq data
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1
|
71
|
August 25, 2025
|
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Effect of primer removal on chimera detection in DADA2
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11
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219
|
September 26, 2025
|
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Shall I need to remove ASV with prevelance in one sample?
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4
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109
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August 8, 2025
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Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)?
|
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4
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174
|
July 22, 2025
|
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Less percentage of reads merged in qiime-DADA2 even with enough overlapping length present.
|
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7
|
120
|
August 11, 2025
|
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dada2 error: rcpp/SparseArray
|
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2
|
49
|
August 10, 2025
|
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PICRUST2 Input Data setting
|
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1
|
139
|
July 8, 2025
|
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Please help interpreting demux data
|
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2
|
74
|
July 18, 2025
|
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Minimum length of read in ASV (DADA2)
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6
|
148
|
July 9, 2025
|
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Shotgun sequencing and Trimming
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2
|
53
|
June 3, 2025
|
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Understanding min sequence length of F and R/ quality scores and how it is impacting dada2 downstream
|
|
15
|
183
|
July 4, 2025
|
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Denoise pair returning DataFrame' object has no attribute 'map' error
|
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6
|
110
|
July 4, 2025
|
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dada2-paired giving return code 1
|
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7
|
95
|
June 28, 2025
|
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DADA2 fails silently on SLURM cluster (QIIME2 2024.10) — No .qza outputs, no error messages
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2
|
58
|
June 23, 2025
|
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Error with DADA2 with sequences of different runs.
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4
|
79
|
June 21, 2025
|
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Can trimming and dada 2
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|
3
|
81
|
May 19, 2025
|
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Low percentage of non-quimeric reads after dada2
|
|
4
|
110
|
June 14, 2025
|
|
Alpha diversity with filtered raw data or unfiltered raw data
|
|
1
|
122
|
April 24, 2025
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