# \#adonis

**URL:** https://forum.qiime2.org/tag/adonis/237.md

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## [adonis beta div statistics](https://forum.qiime2.org/t/adonis-beta-div-statistics/34216)

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**Author:** [@Angeliki](https://forum.qiime2.org/u/Angeliki)\
**Replies:** 2\
**Last updated:** [June 17, 2026, 12:36am UTC](https://forum.qiime2.org/t/adonis-beta-div-statistics/34216 "2026-06-17T00:36:02Z")

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Hi, i am using adonis on my beta diversity results so that i can find which of my metadata categories contributes the most to the results. Every time i run adonis i get different resuts regarding the significance if i c…

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## [How to report more than 3 digits after decimal for ADONIS p value](https://forum.qiime2.org/t/how-to-report-more-than-3-digits-after-decimal-for-adonis-p-value/33313)

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**Author:** [@Shuqi](https://forum.qiime2.org/u/Shuqi)\
**Replies:** 2\
**Last updated:** [June 18, 2025, 1:16pm UTC](https://forum.qiime2.org/t/how-to-report-more-than-3-digits-after-decimal-for-adonis-p-value/33313 "2025-06-18T13:16:39Z")

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Hi, I have revising some of my ADONIS figures and got a request from my PI to add more than 3 digits for p value. And this is because the variable on the spot got a p value as "0.050" which is on the edge. While I get t…

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## [Updating to adonis2](https://forum.qiime2.org/t/updating-to-adonis2/23147)

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**Author:** [@Mehrbod\_Estaki](https://forum.qiime2.org/u/Mehrbod_Estaki)\
**Replies:** 7\
**Last updated:** [October 2, 2023, 6:37pm UTC](https://forum.qiime2.org/t/updating-to-adonis2/23147 "2023-10-02T18:37:48Z")

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Hey folks, I was just wondering if there are any reasons why QIIME 2 couldn't upgrade its adonis() plugin to adonis2() which was introduced after vegan=2.5\_7? The most important benefit would be that adonis2 allows mar…

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## [Adonis error for a specific column](https://forum.qiime2.org/t/adonis-error-for-a-specific-column/27809)

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**Author:** [@turtle](https://forum.qiime2.org/u/turtle)\
**Replies:** 2\
**Last updated:** [September 29, 2023, 8:27pm UTC](https://forum.qiime2.org/t/adonis-error-for-a-specific-column/27809 "2023-09-29T20:27:47Z")

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I have a metadata file where there is a column named "Geological\_location". And I'm trying to performing adonis for every categorical column with the following code, qiime diversity adonis \\ --i-distance-matrix core-m…

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## [ADONIS Df wrong](https://forum.qiime2.org/t/adonis-df-wrong/27739)

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**Author:** [@chiara](https://forum.qiime2.org/u/chiara)\
**Replies:** 6\
**Last updated:** [September 25, 2023, 3:10pm UTC](https://forum.qiime2.org/t/adonis-df-wrong/27739 "2023-09-25T15:10:51Z")

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Hello, I am reaching out for an issue with Adonis. The result gives me a wrong Df per level, it assigns 1 Df instead of 4. Can you please help me to figure out the reason? Is the result reliable? Thank you in advance…

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## [Using PERMANOVA in R to compare unbalanced groups with small sample size](https://forum.qiime2.org/t/using-permanova-in-r-to-compare-unbalanced-groups-with-small-sample-size/26618)

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**Author:** [@col\_ahe](https://forum.qiime2.org/u/col_ahe)\
**Replies:** 5\
**Last updated:** [May 27, 2023, 1:35am UTC](https://forum.qiime2.org/t/using-permanova-in-r-to-compare-unbalanced-groups-with-small-sample-size/26618 "2023-05-27T01:35:13Z")

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Hello everyone! I have been trying to use the pairwiseAdonis tool in R to compare different groups in my QIIME2 output. I converted my filtered SV table file, tree file, taxonomy file, and metadata file from QIIME2 into…

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## [how to take into account sample replicates in beta-group-significance testing?](https://forum.qiime2.org/t/how-to-take-into-account-sample-replicates-in-beta-group-significance-testing/25208)

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**Author:** [@Dot](https://forum.qiime2.org/u/Dot)\
**Replies:** 4\
**Last updated:** [January 14, 2023, 6:30pm UTC](https://forum.qiime2.org/t/how-to-take-into-account-sample-replicates-in-beta-group-significance-testing/25208 "2023-01-14T18:30:22Z")

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Hello, I was wondering if it's necessary to take into account repeated measures or nested relationships when performing beta diversity significance testing, such as with beta-group-significance or adonis? If so, is it p…

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## [Adonis strata with sparse clustering](https://forum.qiime2.org/t/adonis-strata-with-sparse-clustering/24034)

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**Author:** [@naomig](https://forum.qiime2.org/u/naomig)\
**Replies:** 3\
**Last updated:** [August 31, 2022, 10:18pm UTC](https://forum.qiime2.org/t/adonis-strata-with-sparse-clustering/24034 "2022-08-31T22:18:36Z")

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I am running adonis2 in R on my beta diversity matrices and am having trouble deciding whether or not to use the strata argument. We have human saliva 16S sequences from about N=150 people. Because some people have sibl…

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## [ADONIS interpretation sanity check](https://forum.qiime2.org/t/adonis-interpretation-sanity-check/23692)

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**Author:** [@16sIceland](https://forum.qiime2.org/u/16sIceland)\
**Replies:** 4\
**Last updated:** [August 29, 2022, 9:49pm UTC](https://forum.qiime2.org/t/adonis-interpretation-sanity-check/23692 "2022-08-29T21:49:34Z")

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Hello QIIMEers, I'm staring at P-values and starting to question my own sanity. If someone out there can double-check the following interpretations I'd be very grateful! The Experiment: I have microbiome data from an I…

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## [Plugin error from diversity when using adonis plugin](https://forum.qiime2.org/t/plugin-error-from-diversity-when-using-adonis-plugin/23880)

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**Author:** [@pkmnsandy](https://forum.qiime2.org/u/pkmnsandy)\
**Replies:** 3\
**Last updated:** [August 19, 2022, 11:38pm UTC](https://forum.qiime2.org/t/plugin-error-from-diversity-when-using-adonis-plugin/23880 "2022-08-19T23:38:50Z")

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Hi, I am trying to run PERMANOVA using the adonis plugin. Here is my code: qiime diversity adonis \\ --i-distance-matrix output/output/pmoA\_deicode\_distance\_nobulk.qza \\ --m-metadata-file metadata/metadata.txt …

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## [Adonis Plugin Unable to Load Lattice](https://forum.qiime2.org/t/adonis-plugin-unable-to-load-lattice/23581)

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**Author:** [@naomig](https://forum.qiime2.org/u/naomig)\
**Replies:** 4\
**Last updated:** [August 16, 2022, 10:51pm UTC](https://forum.qiime2.org/t/adonis-plugin-unable-to-load-lattice/23581 "2022-08-16T22:51:57Z")

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I have the following error when running qiime diversity adonis: R version 4.2.1 (2022-06-23) Loading required package: permute Loading required package: lattice Error: package or namespace load failed for ‘lattice’ in l…

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## [Runing Adonis with strata](https://forum.qiime2.org/t/runing-adonis-with-strata/23857)

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**Author:** [@timanix](https://forum.qiime2.org/u/timanix)\
**Replies:** 0\
**Last updated:** [August 10, 2022, 7:56am UTC](https://forum.qiime2.org/t/runing-adonis-with-strata/23857 "2022-08-10T07:56:28Z")

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I know that this fucntionality is going to be implemented in one of the new releases of Qiime2, but in case if someone need it before the corresponding update, here is a short tutorial how to perform Adonis analysis with…
