# \#454 **URL:** https://forum.qiime2.org/tag/60-tag/60.md [Latest](https://forum.qiime2.org/latest.md) · [Categories](https://forum.qiime2.org/categories.md) · [Tags](https://forum.qiime2.org/tags.md) --- ## [454 data - Error rates could not be estimated](https://forum.qiime2.org/t/454-data-error-rates-could-not-be-estimated/31868)
**Author:** [@saras224](https://forum.qiime2.org/u/saras224)\ **Replies:** 1\ **Last updated:** [November 12, 2024, 2:26pm UTC](https://forum.qiime2.org/t/454-data-error-rates-could-not-be-estimated/31868 "2024-11-12T14:26:23Z")
Hi @colinbrislawn I have 454 data and I am also getting the same error while trying to denoise the data. I tried using denoise-pyro but I still get the same error that the reads are few. Did you resolve the error? if y… --- ## [Training v4v5 classifier for use with legacy 454 Data](https://forum.qiime2.org/t/training-v4v5-classifier-for-use-with-legacy-454-data/31429)
**Author:** [@454Data](https://forum.qiime2.org/u/454Data)\ **Replies:** 1\ **Last updated:** [September 17, 2024, 6:43pm UTC](https://forum.qiime2.org/t/training-v4v5-classifier-for-use-with-legacy-454-data/31429 "2024-09-17T18:43:44Z")
Hiya Q2 Team! :wave: :qiime2: :sunglasses: I've been working with some legacy 454 data we have, following along with the suggestions made in this helpful post, and it has run beautifully! I removed adapters and primers… --- ## [Pyrosequence work in Qiime2 (Barcode)](https://forum.qiime2.org/t/pyrosequence-work-in-qiime2-barcode/29543)
**Author:** [@You\_y\_Choi](https://forum.qiime2.org/u/You_y_Choi)\ **Replies:** 10\ **Last updated:** [March 15, 2024, 4:37am UTC](https://forum.qiime2.org/t/pyrosequence-work-in-qiime2-barcode/29543 "2024-03-15T04:37:27Z")
Hello, experts. I apologize for missing the reply to my last question. Sorry about that. Here is the link for reference: Pyrosequence work in Qiime2. As mentioned in my earlier question, "I followed this amazing tutori… --- ## [Pyrosequence work in Qiime2](https://forum.qiime2.org/t/pyrosequence-work-in-qiime2/29067)
**Author:** [@You\_y\_Choi](https://forum.qiime2.org/u/You_y_Choi)\ **Replies:** 1\ **Last updated:** [January 28, 2024, 3:10pm UTC](https://forum.qiime2.org/t/pyrosequence-work-in-qiime2/29067 "2024-01-28T15:10:45Z")
Hello, experts. I am working with the pyrosequence dataset in Qiime2. I followed this amazing tutorial (analyzing 454 data in QIIME 2), and while applying it to my data, I encountered a problem with cutadapt. qiime too… --- ## [does anyone know how to work with pyrosequencing reads in qiime2?](https://forum.qiime2.org/t/does-anyone-know-how-to-work-with-pyrosequencing-reads-in-qiime2/24046)
**Author:** [@kevin\_SalOrt](https://forum.qiime2.org/u/kevin_SalOrt)\ **Replies:** 4\ **Last updated:** [October 7, 2022, 11:50pm UTC](https://forum.qiime2.org/t/does-anyone-know-how-to-work-with-pyrosequencing-reads-in-qiime2/24046 "2022-10-07T23:50:31Z")
hello friends! I'm doing an exercise with sequences obtained by pyrosequencing and my first problem was to import them, so, in order to achieve it, I imported them with the help of a manifest file with SingleEndFastqMan… --- ## [Cutadapt not trimming 454 reads](https://forum.qiime2.org/t/cutadapt-not-trimming-454-reads/20247)
**Author:** [@Natali\_Hernandez](https://forum.qiime2.org/u/Natali_Hernandez)\ **Replies:** 7\ **Last updated:** [August 4, 2021, 10:55am UTC](https://forum.qiime2.org/t/cutadapt-not-trimming-454-reads/20247 "2021-08-04T10:55:20Z")
Hi, I'm trying to trim the primers (515F 5′-GTG CCA GCM GCC GCG GTA A-3′ and 806R 5′-GGA CTA CVS GGG TAT CTA AT-3′) of single end 454 reads (downloaded from NCBI) with Cutadapt (qiime2-2020.11). I've used the following: … --- ## [Importing two directions 454 seq data for denoise-pyro](https://forum.qiime2.org/t/importing-two-directions-454-seq-data-for-denoise-pyro/17348)
**Author:** [@Payton](https://forum.qiime2.org/u/Payton)\ **Replies:** 5\ **Last updated:** [November 10, 2020, 5:28pm UTC](https://forum.qiime2.org/t/importing-two-directions-454-seq-data-for-denoise-pyro/17348 "2020-11-10T17:28:34Z")
Hi all, I retrieved 2 directions (forward and reverse) 454 sequencing data (already in fastq format), and would like to use dada2 denoise-pyto function. However, it only supports single file. Is there any ways to combin… --- ## [How to prepare 454 data for dada2 denoise-pyro?](https://forum.qiime2.org/t/how-to-prepare-454-data-for-dada2-denoise-pyro/13314)
**Author:** [@vca007](https://forum.qiime2.org/u/vca007)\ **Replies:** 5\ **Last updated:** [January 23, 2020, 6:49pm UTC](https://forum.qiime2.org/t/how-to-prepare-454-data-for-dada2-denoise-pyro/13314 "2020-01-23T18:49:30Z")
Hi, it is my first time that I play with 454 data (yes I am that young researcher). I am interested into comparing results from other studies so therefore I extracted sff files from SRA. All samples are already demultip… --- ## [Importing 454 data to run dada2 denoise-pyro](https://forum.qiime2.org/t/importing-454-data-to-run-dada2-denoise-pyro/7371)
**Author:** [@Mdavrandi](https://forum.qiime2.org/u/Mdavrandi)\ **Replies:** 3\ **Last updated:** [December 11, 2018, 4:57pm UTC](https://forum.qiime2.org/t/importing-454-data-to-run-dada2-denoise-pyro/7371 "2018-12-11T16:57:40Z")
Hi, I would like to analyse 454 data using QIIME 2, particularly would like to use the denoise-pyro method of DADA2. I have experience working with MiSeq data but I am still struggling to get going with 454 data that I… --- ## [Truncate reads before VSEARCH with pyrosequecing/ion torrent data](https://forum.qiime2.org/t/truncate-reads-before-vsearch-with-pyrosequecing-ion-torrent-data/7122)
**Author:** [@ja.morillo](https://forum.qiime2.org/u/ja.morillo)\ **Replies:** 4\ **Last updated:** [December 11, 2018, 1:51pm UTC](https://forum.qiime2.org/t/truncate-reads-before-vsearch-with-pyrosequecing-ion-torrent-data/7122 "2018-12-11T13:51:32Z")
First, thanks a lot for all the development in qiime2 and the help provided here, you are amazing people! I need to analyze several 454 and ion-torrent datasets. I would like to use clustering-vsearch-otus first, and it… --- ## [Using datasets created with different technologies](https://forum.qiime2.org/t/using-datasets-created-with-different-technologies/4508)
**Author:** [@tenguzame](https://forum.qiime2.org/u/tenguzame)\ **Replies:** 7\ **Last updated:** [June 12, 2018, 10:07pm UTC](https://forum.qiime2.org/t/using-datasets-created-with-different-technologies/4508 "2018-06-12T22:07:58Z")
Hi everybody, I have some different prokaryotic 16S amplicon sequencing datasets I'd like to analyze. However, they have all been created at different times and by means of either 454 pyrosequencing or Illumina MiSeq. A… --- ## [Importing 454 raw reads](https://forum.qiime2.org/t/importing-454-raw-reads/2469)
**Author:** [@LauraMason](https://forum.qiime2.org/u/LauraMason)\ **Replies:** 6\ **Last updated:** [January 19, 2018, 4:21pm UTC](https://forum.qiime2.org/t/importing-454-raw-reads/2469 "2018-01-19T16:21:18Z")
Hello, I would like to use Qiime 2 to analyze 454 data without using Qiime1 first to generate the .biom table. Can anyone think of a way to do this? Is there a way to convert the .fna file to .qza? Thanks! --- ## [Can we analyze 454 generated data?](https://forum.qiime2.org/t/can-we-analyze-454-generated-data/1020)
**Author:** [@drmusk](https://forum.qiime2.org/u/drmusk)\ **Replies:** 1\ **Last updated:** [August 16, 2017, 6:01pm UTC](https://forum.qiime2.org/t/can-we-analyze-454-generated-data/1020 "2017-08-16T18:01:55Z")
Hi I have with me .FNA .QUAL and .TXT with me to analyze but I couldn't find a tutorial for 454 in QIIME2. --- ## [Importing/converting (.SFF) to (FASTA, QUAL ,flowgram) using QIIME2](https://forum.qiime2.org/t/importing-converting-sff-to-fasta-qual-flowgram-using-qiime2/984)
**Author:** [@tlaw](https://forum.qiime2.org/u/tlaw)\ **Replies:** 2\ **Last updated:** [August 14, 2017, 2:50pm UTC](https://forum.qiime2.org/t/importing-converting-sff-to-fasta-qual-flowgram-using-qiime2/984 "2017-08-14T14:50:05Z")
I am looking to import and convert sequencing data (.SFF file format) generated by the Roche 454 GS-FLX+ Genome Sequencer using QIIME2. Previously this was done in QIIME using the 'process\_sff.py' script (process\_sff.py …