Hi all, I am working with samples that are in FASTA format, I imported as indicated in the tutorial "Clustering sequences into OTUs using q2-vsearch" and everything fine, I could also do a taxonomic analysis and a tree and fine, but my doubt is the following: If I have 2 FASTA reads per sample, one forward and one reverse, how can I work as paired format in qiime2 with FASTA data?
FASTQ has nucleotides and quality scores.
FASTA only has nucleotides (no quality scores).
Those quality scores are what makes fastq useful, and those have already been removed from your files.
But your sequencing service should have your raw data, including the full fastq files! Ask them to send the original fastq files to you so you can do the processing yourself in Qiime!