# Workflow for/way to use qiime2 with nanopore data

**URL:** https://forum.qiime2.org/t/workflow-for-way-to-use-qiime2-with-nanopore-data/31363
**Category:** Technical Support
**Tags:** taxonomy, nanopore, import
**Created:** [September 9, 2024, 4:48pm UTC](https://forum.qiime2.org/t/workflow-for-way-to-use-qiime2-with-nanopore-data/31363 "2024-09-09T16:48:10Z")
**Posts on this page:** 1
**Showing post:** 4

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### Author: ![colinbrislawn](https://forum.qiime2.org/user_avatar/forum.qiime2.org/colinbrislawn/32/6221_2.png) [@colinbrislawn](https://forum.qiime2.org/u/colinbrislawn)
#### Post date: [September 11, 2024, 5:27pm UTC](https://forum.qiime2.org/t/workflow-for-way-to-use-qiime2-with-nanopore-data/31363/4 "2024-09-11T17:27:06Z")

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> [@lmcfarland](#):
>
> 5 different primer sets to target specific diet contents (

Okay, wow! That sounds a lot like this Ion Torrent kit, which is also multi-region.

> [@Possible Analysis Pipeline for Ion Torrent 16S Metagenomics Kit Data in QIIME2?](https://forum.qiime2.org/t/possible-analysis-pipeline-for-ion-torrent-16s-metagenomics-kit-data-in-qiime2/13476/):
>
> Hi QIIME2 community, My colleague @Lauren I have been using the Ion torrent 16S Metagenomics Kit to sequence some clinical samples that we have. Our sample sets are both longitudinal. The kit consists of two primer sets, one for V2-4-8 and one for V3-6,7-9. The products of the reactions are pooled per sample, and then the rest of library prep is completed. Each sample gets its own barcode. Ion Torrent produces single end reads. The issue here is that the reads from each of the 6 variable regio…

Multi-region kits have proven to be extraordinarily difficult.

Take a look at that thread and let us know what you want to try next.

There's another Lauren in that thread too, so maybe it's a curse! 👻

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