Workflow for illumina demultiplexed paired end data

Hi @Dharitri,
Import the sequence files together into a single artifact of demultiplexed sequences, and go from there.

Import as described here or here. Either will produce a single artifact of demultiplexed sequences that you can use downstream.

Then you can proceed with qiime dada2 denoise-paired or with OTU picking (if you use dada2 you can trim the primers out with the trim parameters. If you use OTU picking, first use cutadapt trim-paired as described here to trim primers before proceeding).

Good luck!