Which feature table to use after applying the fragment insertion tree method?

Hi Everyone,

I’m trying to get familiar with the q2-fragment-insertion plugin.
In the tutorial, it says that, after using it, we have to filter the input feature-table down to fragments included in the phylogeny.
So, from this point forward, I should only use the filtered_table.qza and insertion-tree.qza for all downstream analyses, including alpha and beta diversity analysis and to make the taxa bar plots? Would this be correct?

Thanks very much,
FS

Hi @fstudart,

Yes, you need the features in your tree to match the features in your table and your taxonomy file. Specifically, each row in your feature table (ASV or OTU) must have a corresponding tip in the tree and taxonomic name. However, not every tip must have a feature. …Not sure if that makes sense.
But, yes, you do need the paired set for all downstream analyses.

Do you have a reason to believe that the features from your table weren’t included in the tree building?

Best,
Justine

Hi Justine,

Thanks very much for your feedback. No specific reason. Just curious.

Thanks,
FS

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