Hey guys, I’ve just started to get the hang of qiime2 but I am having trouble deciding were to truncate the reads in DADA2, especially. I have attached the screenshot as shown
The quality of the forward reads look good whereas the quality of the reverse end reads is lower and I don’t know where to truncate. I’d really appreciate if anyone could provide me with support. For the forward reads I was thinking of triming 62 from the left and a trunc of 280 and for the reverse reads trim left 62 and trunc 250. My worry is the parameters I selected could lead to overtrim/undertrim.