# where to cut this sequence from my demux.qzv

**URL:** https://forum.qiime2.org/t/where-to-cut-this-sequence-from-my-demux-qzv/29261
**Category:** User Support
**Created:** [February 14, 2024, 2:11pm UTC](https://forum.qiime2.org/t/where-to-cut-this-sequence-from-my-demux-qzv/29261 "2024-02-14T14:11:12Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![Giulianafini](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/g/f04885/32.png) [@Giulianafini](https://forum.qiime2.org/u/Giulianafini)
#### Post date: [February 14, 2024, 2:11pm UTC](https://forum.qiime2.org/t/where-to-cut-this-sequence-from-my-demux-qzv/29261/1 "2024-02-14T14:11:12Z")

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where should I cut my data?  
I don't want to lose too much data and overlap

 ![Screenshot 2024-02-14 at 8.55.38 AM](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/d/e/dece1f3797bad54886300cd8bf598b04c5684348.png)

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### Author: ![colinbrislawn](https://forum.qiime2.org/user_avatar/forum.qiime2.org/colinbrislawn/32/6221_2.png) [@colinbrislawn](https://forum.qiime2.org/u/colinbrislawn)
#### Post date: [February 14, 2024, 2:14pm UTC](https://forum.qiime2.org/t/where-to-cut-this-sequence-from-my-demux-qzv/29261/2 "2024-02-14T14:14:50Z")

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Hi Giulianafini,

Welcome to the forums! :qiime2:

This is an important question because keeping more data is good!

In case you have not found this already, here is how I approach this question:

> [@Merging, Quality control and overlapping](https://forum.qiime2.org/t/merging-quality-control-and-overlapping/12618):
>
> Hello to everyone. I have some questions related to qiime2 and I would appreciate it if you can help me out please. First of all, I have to say that one part of my question is similar to an old post in qiime forum ([problem with merging step in qiime2 with dada2](https://forum.qiime2.org/t/problem-with-merging-step-in-qiime2-with-dada2/11251)) but since those explanations weren’t clear for me I will ask again. I try to give details as much as I can. I am analyzing the 16S data and I have 48 paired-end samples. The primers are: 16S-341F and 16S-805R. The questions are : 1-…

What settings have you tried already and how well do they work?

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [March 16, 2024, 8:15pm UTC](https://forum.qiime2.org/t/where-to-cut-this-sequence-from-my-demux-qzv/29261/3 "2024-03-16T20:15:06Z")

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