I have a question about the statistics used in the Qiime 2. I have Two different soils, One is peat and other is clay and silty type and 4-time points, 2 months, 4 months, 6 months, 8 months. After two months I extract DNA from each soil, I send to the company, then after 4 months then 6 months and 8 months. I want to compare time microbiome within the time points of each soil i.e. 2 months with 4 months, 4 months with 6 months and 6 months with 8 months and want to compare timepoints between the two types of soil like 2 months of one soil with the two months of other soil. Which statistical analysis can I use in Qime 2 for this type of experiment? Can I use permanova or Ancom as in the moving material tutorial?
Waiting for assistance!