Hi. I follow two tutorials. one of them goes directly to building a tree working with mafft program and the other does intermediate steps as blast classifier and metadata tabulate. I prefer the simple steps as I am now practicing. Also, my reference sequences are from HOMD. Regards
You can do these two steps in either order, because the results are independant. This is a ‘branching’ path of your analysis.
/--> build tree import --> denoise-> | \--> classify taxonomy
I hope that helps!
Classify your reads using the SILVA database, the try HOMD as an alternative database.
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