Ways to assess absolute levels of microbes within samples


I am trying to assess the absolute levels of microbes (non-unique) within samples. I have tried comparing the number of features in the table.qza. But I was wondering if there are any other methods to compare this!

Thank you in advance!

Hi @mhk,
Spike-in controls (in your sequencing run)
flow cytometry

High-throughput sequencing data (e.g., marker-gene and metagenome data) do not provide absolute abundance measurements.

Good luck!

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