Hello!
I am trying to assess the absolute levels of microbes (non-unique) within samples. I have tried comparing the number of features in the table.qza. But I was wondering if there are any other methods to compare this!
Thank you in advance!
Hello!
I am trying to assess the absolute levels of microbes (non-unique) within samples. I have tried comparing the number of features in the table.qza. But I was wondering if there are any other methods to compare this!
Thank you in advance!
Hi @mhk,
QPCR
Spike-in controls (in your sequencing run)
flow cytometry
High-throughput sequencing data (e.g., marker-gene and metagenome data) do not provide absolute abundance measurements.
Good luck!