I`m having a problem with qiime feature-classifier classify-consensus-vsearch. I'm trying to assign taxonomy on a dataset using the 99 SILVA database for primers 515F and 806bR, however for some reason all of the features are classified as unassigned. To test if there was a problem with my data, I went back and tried to re-run the classification on an old dataset I'd previously classified (using the 2018 qiime release), with the same reference sequences and taxonomy, but those features also get classified as unassigned.
I'm using default parameters on vsearch in qiime2-2019.4
Thank you for any advice
query sequences: rep-seqs.qza
Reference seq and taxonomy: Dropbox - Reference - Simplify your life
rep-seqs.qza (8.9 KB)