# Volatility Control Chart Spaghetti lines **URL:** https://forum.qiime2.org/t/volatility-control-chart-spaghetti-lines/31133 **Category:** User Support **Tags:** longitudinal **Created:** [August 14, 2024, 9:08am UTC](https://forum.qiime2.org/t/volatility-control-chart-spaghetti-lines/31133 "2024-08-14T09:08:22Z") **Posts on this page:** 6 **Page:** 1
### Author: ![elina2410](https://forum.qiime2.org/user_avatar/forum.qiime2.org/elina2410/32/16280_2.png) [@elina2410](https://forum.qiime2.org/u/elina2410) #### Post date: [August 14, 2024, 9:08am UTC](https://forum.qiime2.org/t/volatility-control-chart-spaghetti-lines/31133/1 "2024-08-14T09:08:22Z")
Hi! I use qiime2-amplicon-2023.9. I performed longitudinal volatility analysis and obtained these charts: when I want to visualize all samples without collapsing: ![obraz](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/e/1/e1513e054420fb1863a1a3711458ad6544f8e399.png) after collapsing: ![obraz](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/e/8/e8b122f7e74be8cb38b7125d0a8e59553ad7a5cd.png) Why there are no lines here in both charts? and after further collapsing: ![obraz](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/c/8/c871ce663fae9cff5d2dac4996d96bd816ee735a.png). and why there are no Spaghetti lines here? Only mean line. The same kind of analysis on this data I performed last year but probably with qiime2-2022.8 and slightly different rarefying. Here are the charts without collapsing: ![obraz](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/d/4/d4dc8bc3ac918d890c438a747ca850d28e9350a2.png) and after collapsing: ![obraz](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/1/e/1e5ef0fbe6b22b80d4dcf0d4a2c75f2fc35b3f22.png) ![obraz](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/b/d/bd052aaa1f7915045f81654b36ae1b51d917c6dd.png) What is the reason for no spagetti lines in the recent analysis? Could it be connected with this issue [Volatility Control Chart visualizing issue](https://forum.qiime2.org/t/volatility-control-chart-visualizing-issue/9569) Best, E ---
### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich) #### Post date: [August 15, 2024, 6:29am UTC](https://forum.qiime2.org/t/volatility-control-chart-spaghetti-lines/31133/2 "2024-08-15T06:29:42Z")
Hi @elina2410 , Could you please upload the QZV and share the command that you used to create this QZV? > [@elina2410](#): > > Could it be connected with this issue [Volatility Control Chart visualizing issue](https://forum.qiime2.org/t/volatility-control-chart-visualizing-issue/9569) I do not think so — that issue is specifically caused by really long feature names causing the plot to rescale. So I do not see any connection here, since your feature IDs are not long. ---
### Author: ![elina2410](https://forum.qiime2.org/user_avatar/forum.qiime2.org/elina2410/32/16280_2.png) [@elina2410](https://forum.qiime2.org/u/elina2410) #### Post date: [August 16, 2024, 7:16am UTC](https://forum.qiime2.org/t/volatility-control-chart-spaghetti-lines/31133/3 "2024-08-16T07:16:02Z")
Hi @Nicholas_Bokulich Here are the command I used and the qzv file obtained: qiime longitudinal volatility --m-metadata-file metadane.txt core-metrics-results/shannon.qza --p-default-metric shannon\_entropy --p-default-group-column antibiotic --p-state-column week --p-individual-id-column new\_id --o-visualization core-metrics-results/volatility.qzv [volatility.qzv](https://forum.qiime2.org/uploads/short-url/2jnReMBaeV9ORdqVzWeMFI0fFuR.qzv) (509.7 KB) E. ---
### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich) #### Post date: [August 16, 2024, 8:43am UTC](https://forum.qiime2.org/t/volatility-control-chart-spaghetti-lines/31133/4 "2024-08-16T08:43:13Z")
Hi @elina2410 , Thank you for sharing these. It looks like the issue is that `new_id` consists entirely of unique values. So none of these are repeated, and hence no spaghetti lines can be drawn. The `individual-id-column` should indicate the IDs of subjects that were sampled repeatedly (or if not unique subjects, then some ID that links the samples that you want to connect). I hope that helps! ---
### Author: ![elina2410](https://forum.qiime2.org/user_avatar/forum.qiime2.org/elina2410/32/16280_2.png) [@elina2410](https://forum.qiime2.org/u/elina2410) #### Post date: [August 16, 2024, 12:50pm UTC](https://forum.qiime2.org/t/volatility-control-chart-spaghetti-lines/31133/5 "2024-08-16T12:50:57Z")
Thank you @Nicholas_Bokulich, I thought that each spaghetti line visualizes single sample... I should check tutorial again, since I performed this analysyis long time ago (and it worked then, but probably I used antibiotic column instead of new\_id ) Thank you again! E. ---
### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system) #### Post date: [September 16, 2024, 6:51pm UTC](https://forum.qiime2.org/t/volatility-control-chart-spaghetti-lines/31133/6 "2024-09-16T18:51:42Z")
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