Very long time importing fastq files with Phred64

Technical Support
1

Hi,

I am trying to import my data with phred64 offset, however, it is taking a very long time even when I try with just two files (forward and reverse). I have attached my manifest file and shared the link for the mentioned fastq files.

I am using qiime2-2019.7, with this command :

qiime tools import
--type 'SampleData[PairedEndSequencesWithQuality]
--input-path manifest_toy.tsv
--output-path paired-end-demux_toy.qza
--input-format PairedEndFastqManifestPhred64V2

manifest_toy.tsv (104 Bytes)
files : toy - Google Drive

I was wondering if this is expected?

Thanks in advance,
Parizad

1 Like
2

Yep, I think so! This is a pretty intensive process, since the import converts from PHRED 64 to PHRED 33. Just let it cook for a while :stew:!

:t_rex:

1 Like
3

Alright! :slight_smile:
Would it be help if I convert Phred64 to 33 before importing or will it be basically the same thing?
I was also curious if working on cluster would increase the speed of this process?

4

It will be exactly the same thing.

No, I doubt it --- this process can't be parallelized (yet) in QIIME 2. Just let it cook.

1 Like