# Using RESCRIPt's 'extract-seq-segments' to extract reference sequences without PCR primer pairs.

**URL:** https://forum.qiime2.org/t/using-rescripts-extract-seq-segments-to-extract-reference-sequences-without-pcr-primer-pairs/23618
**Category:** Tutorials
**Tags:** taxonomy, feature-classifier, trnl, lsu, its, ssu, co1, tutorial, rescript
**Created:** [July 13, 2022, 4:18pm UTC](https://forum.qiime2.org/t/using-rescripts-extract-seq-segments-to-extract-reference-sequences-without-pcr-primer-pairs/23618 "2022-07-13T16:18:36Z")
**Posts on this page:** 1
**Showing post:** 16

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### Author: ![SoilRotifer](https://forum.qiime2.org/user_avatar/forum.qiime2.org/soilrotifer/32/21071_2.png) [@SoilRotifer](https://forum.qiime2.org/u/SoilRotifer)
#### Post date: [August 17, 2026, 3:27pm UTC](https://forum.qiime2.org/t/using-rescripts-extract-seq-segments-to-extract-reference-sequences-without-pcr-primer-pairs/23618/16 "2026-08-17T15:27:27Z")

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2 off-topic replies have been split into a new topic: [extract-seq-segments: number of reads vs accuracy of reads.](https://forum.qiime2.org/t/extract-seq-segments-number-of-reads-vs-accuracy-of-reads/34293)

Please keep replies on-topic in the future.

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_[View the full topic](https://forum.qiime2.org/t/using-rescripts-extract-seq-segments-to-extract-reference-sequences-without-pcr-primer-pairs/23618)._