# Using QIIME2 to view 454 pyrosequencing data

**URL:** https://forum.qiime2.org/t/using-qiime2-to-view-454-pyrosequencing-data/17500
**Category:** General Discussion
**Tags:** import
**Created:** [November 18, 2020, 6:28am UTC](https://forum.qiime2.org/t/using-qiime2-to-view-454-pyrosequencing-data/17500 "2020-11-18T06:28:54Z")
**Posts on this page:** 1
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### Author: ![perdita](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/p/ebca7d/32.png) [@perdita](https://forum.qiime2.org/u/perdita)
#### Post date: [November 19, 2020, 12:01am UTC](https://forum.qiime2.org/t/using-qiime2-to-view-454-pyrosequencing-data/17500/3 "2020-11-19T00:01:39Z")

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> [@andrewsanchez](#):
>
> Please correct me if I am mistaken, but it seems to me that the only relevant portion of this diagram is the upper left corner which shows that the 454 Sequencer generated .fna/.qual files. Given that you have the .fna/.qual files, and no\* the results of the rest of that analysis process (right?), I don't think the rest of the diagram is relevant. Does that make sense or am I missing something?

Thank you for the reply! You are correct that I am picking up at the .fna/.qual node of the flowchart, however other versions of this lab's charts include "FASTA / Qual Prepared for QIIME" as an end step from the "Quality Checking / Demultiplexing" node, so I am trying to figure out what I am working with.

 ![Screenshot_20201014_181408_methodology_overview](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/7/733871c2cb53703f6f3ffab19a42e2d103d0fce0.png)

From [Importing and Demultiplexing Sequence Data Quick Reference](https://forum.qiime2.org/t/importing-and-demultiplexing-sequence-data-quick-reference/14002), and the [QIIME2 Import Tutorial](https://docs.qiime2.org/2020.8/tutorials/importing/#sequence-data-with-sequence-quality-information-i-e-fastq), it appears that I need to know if the data are multiplexed or not.

Where I am at:  
✅ Barcodes in Sequence (If they are only at the beginning, does that always imply single-end?)  
❓ Multiplexed? I think it is multiplexed, as several sequences share the same Barcode?  
❓ Need to strip out Barcodes to a metadata file for QIIME2 import?

Thanks for your time and input.

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