# Using QIIME2 to view 454 pyrosequencing data

**URL:** https://forum.qiime2.org/t/using-qiime2-to-view-454-pyrosequencing-data/17500
**Category:** General Discussion
**Tags:** import
**Created:** [November 18, 2020, 6:28am UTC](https://forum.qiime2.org/t/using-qiime2-to-view-454-pyrosequencing-data/17500 "2020-11-18T06:28:54Z")
**Posts on this page:** 1
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### Author: ![andrewsanchez](https://forum.qiime2.org/user_avatar/forum.qiime2.org/andrewsanchez/32/20160_2.png) [@andrewsanchez](https://forum.qiime2.org/u/andrewsanchez)
#### Post date: [November 18, 2020, 11:19pm UTC](https://forum.qiime2.org/t/using-qiime2-to-view-454-pyrosequencing-data/17500/2 "2020-11-18T23:19:00Z")

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Hi, @perdita!

> [@perdita](#):
>
> This is an overview of RaT processing that was done before data delivery at the time, from the document that arrived with the samples.

Please correct me if I am mistaken, but it seems to me that the only relevant portion of this diagram is the upper left corner which shows that the 454 Sequencer generated .fna/.qual files. Given that you have the .fna/.qual files, and no\* the results of the rest of that analysis process (right?), I don't think the rest of the diagram is relevant. Does that make sense or am I missing something?

> [@perdita](#):
>
> Is there an easy way to see whether this is single end or paired end?

It appears that the question is not so straightforward. Please see this [discussion on biostars](https://www.biostars.org/p/111047/).

I need to do a bit more research to answer your other questions confidently. But elsewhere on the forum, people have discussed importing this type of data using a manifest file. For example, see this post: [Importing 454 data to run dada2 denoise-pyro - #3 by Mdavrandi](https://forum.qiime2.org/t/importing-454-data-to-run-dada2-denoise-pyro/7371/3)

You might also look into using q2-cutadapt for demultiplexing if required.

Also, depending on what you need to do, you might have [other options](https://forum.qiime2.org/t/importing-454-raw-reads/2469/3).

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