# Using Cutadapt w 16s Primers

**URL:** https://forum.qiime2.org/t/using-cutadapt-w-16s-primers/10761
**Category:** User Support
**Tags:** dada2
**Created:** [July 19, 2019, 8:36pm UTC](https://forum.qiime2.org/t/using-cutadapt-w-16s-primers/10761 "2019-07-19T20:36:39Z")
**Posts on this page:** 1
**Showing post:** 2

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [July 19, 2019, 9:57pm UTC](https://forum.qiime2.org/t/using-cutadapt-w-16s-primers/10761/2 "2019-07-19T21:57:15Z")

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> [@fabipc](#):
>
> Could it be that I did something wrong, or does cutadapt not trim the primers off the 16s, in general?

cutadapt works the same on any type of DNA sequence data — it is agnostic to marker gene.

Are you sure you have the primers in your reads? E.g., if you used the EMP format the primers are not found in the reads because the PCR primers are also used as the sequencing primer. I recommend checking out the fastq to make sure.

> [@fabipc](#):
>
> \*\*not sure why the \ are not showing on the code on here, but they were included when the code was processed.

Use three backtick characters ("```") before and after all code blocks to display "preformatted text" (or click on the formatting button at the top of the text box that looks like this: `</>` and type your text inside).

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