Hi, I used the following commonds to test differential abundance between groups at the taxnomic level 4 :
qiime gneiss balance-taxonomy
--i-table ./comp-table.qza
--i-tree hierarchy.qza
--i-taxonomy ./taxonomy.qza
--p-taxa-level 4
--p-balance-name 'y0'
--m-metadata-file ./manifest.xls
--m-metadata-column group_manner_1
--o-visualization group_manner_1-y0_taxa_summary-L4.qzv
qiime tools export --input-path group_manner_1-y0_taxa_summary-L4.qzv --output-path group_manner_1-y0_taxa_summary-L4
However, I found many unssigned sequenced at taxnomic level4 appears at the final results:
e2758f390d79b62de4c70d267735d645,Unassigned,Unassigned,Unassigned,Unassigned,Unassigned,Unassigned,Unassigned
0c4b6ae1fd659ce65a1e29a0c8dcbd19,D_0__Archaea,D_1__Nanoarchaeaeota,D_2__Woesearchaeia,D_2__Woesearchaeia,D_2__Woesearchaeia,D_2__Woesearchaeia,D_2__Woesearchaeia
It seems that qiime2 automatically supplement the unsigned taxonomic level the previous taxonomic level. It really confused me a lot.
I think it is better to remove these unsigned results since I can chose the
parameter "--p-taxa-level" to test the coresponding taxonomic levels.
Is there anything qiime can do to remove these unsigned features?
Or should I remove the sequences unannotated at level 4 before doing gneiss taxa_summary analysis ?
Thanks