I’m a newbie in this field and I’m trying to conver a bunch of these demultiplexed .fasq files to a single .qza file.
The publishers of the dataset state that they followed the Earth Microbiome Protocil, ,which I think means that they did pair-end sequencing:
Samples were sequenced according to the Earth Microbiome Project protocols (Gilbert et al., 2014). Briefly, DNA was extracted using a MoBio Power soil kit (Carlsbad, CA), and the V4 region of the 16S rRNA gene was amplified using barcoded primers
(Walters et al., 2015). Sequencing was performed using an Illumina MiSeq.
However, when I downloaded the data from https://www.ebi.ac.uk/ena/data/view/PRJEB17694 page (Bulk Download Files button -> in submitted form), I couldn’t find any trace of pair-end sequencing.
These are the files:
This is what’s inside:
How can I distinguish between forward and backward reads and what am I missing?
https://qiita.ucsd.edu/study/description/10483# - link with the metadata, but no raw sequences
https://www.ebi.ac.uk/ena/data/view/PRJEB17694 - only raw sequences, downloaded in the Submitted file format