Understanding results from closed-reference OTU clustering

Hello!

I’m currently running qiime2-2025.10 and have just successfully aligned sequences from 9 samples to a reference database. I am now presented with the following, once I visualize it:

My question is - is there a way to know which of the 9 samples the “qseqid” comes from?

TIA!

@ThatGuySam, Not directly, but can you describe what your goal is for accessing that information? There may be another recommendation that we could make.

You may know this already (apologies if so), but the FeatureTable that is generated as output from that step tabulates the reference feature ids on a per-sample basis. That's generally the information people are more interested in at this stage.

Oh - I infact did not know that. That is exactly what I was looking for. Thanks Greg!

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You're welcome! If you want to visualize the table itself, you could do that by running the following command (which assumes your table is named table.qza):

qiime metadata tabulate --m-input-file table.qza --o-visualization table.qzv

You can then load the result with QIIME 2 View. This generally isn't the most useful visualization because the tables are big, but just wanted to mention this in case it's helpful.

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That is a common point of confusion when looking at alignment outputs. Usually, you would need to cross-reference your feature table to see how those IDs map across your samples, as the visualization primarily focuses on the sequence identifiers themselves.

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