Unable to find manifest file

[elsamdea]

Hi @cab!

@cab

I am trying to do my first import of fastq files into Qiime2 and keep getting the error that the path to the manifest file does not exist.

First, you are trying to import your fastq and manifest files: in the input-path parameter, you should include the absolute directory path to the q_trimmed_seqs folder (without enter inside the folder), that would avoid problems (you can use the cd command to enter in the folder and the pwd in the terminal to find out what is your absolute directory path). And also, the fastq files should be in a compress form as fastq.gz files.

So then, the command should be:

qiime tools import --type 'SampleData[SequencesWithQuality]'  --input-path "$PWD/qtrimmedseqs/" --input-format SingleEndFastqManifiestPhred33 --output-path "thenameyouwant.qza"

(In the output-path parameter, you can use the name you prefer ).

@cab

If I make a directory in Qiime2 with the same name as on my hard-drive where the data is, will Qiime2 access my hard-drive or will the directory I made in Qiime2 only be useable within the Qiime2 environment? If the latter, how do I tell Qiime2 where the data is?

About that, I guess you are using the Linux subsystem inside Windows... And maybe this post could help you:

Permission denied when importing FASTQ files

Hi @taf1g17, This is very helpful thank you! As I mentioned earlier is this thread, you can not use Windows-style file paths, but the file paths as WSL understands them. The best way to make sure you are using the correct Linux file paths is to cd into the folder where your sequence files are located, and type pwd. This will provide you with the absolute directory path. Do this within the WSL Terminal of course. taf1g17$: cd share/seqs-to-import taf1g17:~/share/seqs-to-import$ pwd …

I hope I have been helpful!

Best,

Elsa