Trouble with ITSxpress into dada2

User Support
1

Hey all,

I am trying to get my ITS samples into a taxa bar plot and am running into some issues when going from the ITSxpress step into dada2. The ITSxpress step ran successfully and spit out a .qza file which I want to put into dada2. The problem I have is that I don't know what length my trimmed sequences now are to tell dada2.

Ideally, I don't want dada2 to trim anything since ITSxpress already did. I set my trunc length to 0 for my f and r reads and it came back with an error.

Is there any way I can determine what the length of my reads are post ITSxpress or is there a way to run dada2 without trimming anything?

3

Hi @Sean,

Welcome to the :qiime2: forum!

Can you please provide the following information:

  • The version of QIIME 2 you are running
  • The machine/OS you are using to run QIIME 2 on
  • All of the commands that you ran in QIIME 2, along with the error message you received (copy and paste these please!)

Thanks!

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