I am trying to get my ITS samples into a taxa bar plot and am running into some issues when going from the ITSxpress step into dada2. The ITSxpress step ran successfully and spit out a .qza file which I want to put into dada2. The problem I have is that I don't know what length my trimmed sequences now are to tell dada2.
Ideally, I don't want dada2 to trim anything since ITSxpress already did. I set my trunc length to 0 for my f and r reads and it came back with an error.
Is there any way I can determine what the length of my reads are post ITSxpress or is there a way to run dada2 without trimming anything?