Trimming help with DADA2 - what would be the best

What would be the best trim positions for this data?
demux.qzv (313.3 KB)

Hi @rpaudel,
We have a your work is your own policy on the forum, so I can not tell you where to trim your data.

I can tell you there rule thumb for the truncating is to trim around where the median quality score of your data is falling below 30.

I would try trimming at a couple of locations if you are unsure!

Feel free to come back to the QIIME 2 forum for help interpreting the outputs, But make sure that you share your interpretation and what is confusing before asking for help!

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