Trim and Trunc error

Hi all, i had been encountering this error. had checked previous issues but yet seem not to be resolved. here is my command and error
qiime dada2 denoise-single
--i-demultiplexed-seqs demux.qza
--p-trim-left-f 20
--p-trim-left-r 20
--p-trunc-len-f 295
--p-trunc-len-r 234
--o-representative-sequences rep-seqs-dada2.qza
--o-table table-dada2.qza
--o-denoising-stats stats-dada2.qza
But comes with these errors
(1/4?) no such option: --p-trim-left-f Did you mean --p-trim-left?
(2/4?) no such option: --p-trim-left-r Did you mean --p-trim-left?
(3/4?) no such option: --p-trunc-len-f Did you mean --p-trunc-len?
(4/4?) no such option: --p-trunc-len-r Did you mean --p-trunc-len?
Please any suggestions?

Hi @pyghost,

The issue is that you are using denoise-paired options that do not exist for denoise-single.

To see which options are available while you're working in the terminal you can simply add --help to the end of your command. For example, when you enter the command below, you'll see the help text that describes the available options:

qiime dada2 denoise-single --help

-Cheers!
-Mike

1 Like

Thanks a lot for swift response. I was totally blinded off that command because i was following the moving picture tutorial. Thanks a lot again.

2 Likes

This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.