Training for 515F/806R sequences

I have 16S Illumina sequencing data from mouse stool samples. I used the original Caporaso et al. 515F/806R primers. Would you recommend generating my own classifier or can I use gg-13-8-99-515-806-nb-classifier.qza provided?

Hi @pishchg,

Welcome to the forum!

Personally, I’d use the pre-trained classifier for your hypervariable region. I might be careful with single end vs paired end, but I don’t think that should matter.

Once you have a classifier that works with your version of QIIME and primer set, you definitely don’t have to re-train it; my group has one classifier for our primer pair and that’s what we run.



Thanks, Justine! That’s super helpful.

1 Like

This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.