Training feature classifiers with q2-feature-classifier using degenerate primer against V4 region

[Yogesh_Gupta (Yogesh)]

Hi,

we used degenerate primer for 16s V4 region (F515/R806) amplification, can I use same primer set for feature classifier:

qiime feature-classifier extract-reads --i-sequences 99_otus.qza --p-f-primer GTGCCAGCMGCCGCGGTAA --p-r-primer GGACTACHVGGGTWTCTAAT --p-min-length 200 --p-max-length 500 --o-reads ref-seqs.qza

Kind Regards
Yogesh

[Mehrbod_Estaki]

Hi @Yogesh_Gupta,
You sure can!