# Too many unassigned or only at kingdom level features **URL:** https://forum.qiime2.org/t/too-many-unassigned-or-only-at-kingdom-level-features/2934 **Category:** User Support **Tags:** taxonomy **Created:** [February 7, 2018, 8:08pm UTC](https://forum.qiime2.org/t/too-many-unassigned-or-only-at-kingdom-level-features/2934 "2018-02-07T20:08:31Z") **Posts on this page:** 1 **Showing post:** 8
### Author: ![colinbrislawn](https://forum.qiime2.org/user_avatar/forum.qiime2.org/colinbrislawn/32/6221_2.png) [@colinbrislawn](https://forum.qiime2.org/u/colinbrislawn) #### Post date: [February 8, 2018, 11:44pm UTC](https://forum.qiime2.org/t/too-many-unassigned-or-only-at-kingdom-level-features/2934/8 "2018-02-08T23:44:38Z")
Hello again Mehrbod, Thanks for keeping us posted on your findings. 95% is huge! What a disappointment... If **4) off-target effects** is to blame, you can verify that by BLASTing some of your most abundant unidentified reads against the mouse genome on the [NCBI website](https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch). Or maybe they are from the human genome, and you can search for that too. At one point, a collaborator was having trouble with their MiSeq and lot's of Phix was ending up in their samples. Illumina spikes in the Phix bacteriophage as a positive control, so maybe these are the source of your reads. You could search your reads against [the Phix genome](https://www.ncbi.nlm.nih.gov/nucleotide/NC_001422) to see if this is happening. It's a science mystery! 🔬🔍 Colin --- _[View the full topic](https://forum.qiime2.org/t/too-many-unassigned-or-only-at-kingdom-level-features/2934)._