To dereplicate and not to dereplicate (16s sequencing DNA data)

[angelrica_kuan]

Dear all,

Hello, I have a question arise from the vsearch dereplicate.

For an example, I have 10 groups of reads. What if I go through further analysis instead of dereplicating procedure (skipped dereplicating step).

Thank you for your reply.

Sincerely,
Angelrica

[thermokarst]

Hi @angelrica_kuan!

@angelrica_kuan

What if I go through further analysis instead of dereplicating procedure (skipped dereplicating step).

Most downstream analyses require you to have multiple samples (really any dataset with less than 2 samples is going to be very limited) - so I encourage you to run q2-vsearch's dereplicate-sequences action! As well, you'll need at least a feature table for most downstream steps, so that's another reason to not skip this step.

[angelrica_kuan]

Hi @thermokarst

Noted. Thank you very much !!

Its really helpful !!

Angelrica Lee