The problem about the fastq data uploading QIIME2


it is the first time we uploading our data to QIIME2 , but failed to upload and creat .qza so many times and can not find reason.
the error code is
An unexpected error has occurred:

’utf-8’ codec can’t decode byte 0xff in position 0: invalid start byte

and we already created our “manifest” as picture

if you know how to fix it please help me! and thanks you very much
PS:my data is already demultiplexed and is pair-end data

Hey @Doc.chen!

Welcome to the forum!

Would you be able to upload your manifest file to the forum as an attachment?

I strongly suspect that your file is UTF-16-le (instead of UTF-8) as 0xff is the start of a byte-order mark for UTF-16-le. When you saved the file, what editor did you use, and what option to save (screenshot would be great)?

thanks for your help!i used excel to saved this file and saved as txt.
se-33-manifest.txt (836 Bytes)

Thanks @Doc.chen!

Just as I suspected, you are saving as UTF-16 Unicode (Little-endian). In that dropdown, is there an equivalent txt format for UTF-8 Unicode (.txt)?

(I’m guessing a little bit as to what the specific name is)

If there is a UTF-8 option, try saving it using that and see if that fixes the issue.