# The number of features is normal but the number of observed\_otus is too low?

**URL:** https://forum.qiime2.org/t/the-number-of-features-is-normal-but-the-number-of-observed-otus-is-too-low/11625
**Category:** General Discussion
**Tags:** dada2
**Created:** [September 23, 2019, 1:44am UTC](https://forum.qiime2.org/t/the-number-of-features-is-normal-but-the-number-of-observed-otus-is-too-low/11625 "2019-09-23T01:44:11Z")
**Posts on this page:** 1
**Showing post:** 26

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### Author: ![Decen](https://forum.qiime2.org/user_avatar/forum.qiime2.org/decen/32/5086_2.png) [@Decen](https://forum.qiime2.org/u/Decen)
#### Post date: [September 27, 2019, 1:14am UTC](https://forum.qiime2.org/t/the-number-of-features-is-normal-but-the-number-of-observed-otus-is-too-low/11625/26 "2019-09-27T01:14:27Z")

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Hi Yue,

I found someting interesting in your primers for V3-V4 region. Could you please tell me what's the length of your amplicons or the location of the primer pair? what kind of platform did you use for NGS (Miseq or Hiseq or Nova 6000)? Thanks

Best,

Decen

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